Fabrication of porous extracellular matrix scaffolds from human adipose tissue.
Adipose tissue is found over the whole body and easily obtained in large quantities with minimal risk by a common surgical operation, liposuction. Although liposuction was originally intended for the removal of undesired adipose tissue, it may provide an ideal material for tissue engineering scaffolds. Here we present novel, porous scaffolds prepared from human adipose tissues. The scaffolds were fabricated in a variety of macroscopic shapes such as round dishes, squares, hollow tubes, and beads. The microscopic inner porous structure was controlled by the freezing temperature, with a decrease in pore size as the freezing temperature decreased. The scaffold prepared from human adipose tissue contains extracellular matrix components including collagen. Preliminary in vitro studies showed that human adipose-derived stem cells attached to a human extracellular matrix scaffold and proliferated. This scaffold based on human adipose tissue holds great promise for many clinical applications in regenerative medicine, particularly in patients requiring soft-tissue regeneration.
Choi
Ji Suk
JS
Department of Chemical Engineering and Bionanotechnology, Hanyang University, Ansan, Republic of Korea .
Yang
Hyun-Jin
HJ
Kim
Beob Soo
BS
Kim
Jae Dong
JD
Lee
Sang Hoon
SH
Lee
Eun Kyu
EK
Park
Kinam
K
Cho
Yong Woo
YW
Lee
Hee Young
HY
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000328:Adult; D001483:Base Sequence; D001672:Biocompatible Materials; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D017931:DNA Primers; D005109:Extracellular Matrix; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering
D001672:Biocompatible Materials; D017931:DNA Primers; D003094:Collagen