title: Actin-like filaments in synaptosomes detected by heavy meromyosin binding.
abstract: We have prepared cat forebrain synaptosomes in order to investigate the presence of actin-like proteins. First, actin-like filaments were demonstrated using a heavy meromyosin binding technique. Decorated filaments form networks inside the synaptosomes and are frequently seen in relation to membranes and synaptic vesicles. Second, using SDS-polyacrylamide gel electrophoresis, a band corresponding to actin appears to be one of the major constituents of synaptosomal fractions; this confirms the work of Fine and his coworkers. The significance of actin-like filaments in relation to axoplasmic transport is briefly discussed.
authors: Department of Cell Biology, Catholic University of Chile, P.O. Box 114-D, Santiago Chile; Laboratory of Neurophysiology, Catholic University of Chile, P.O. Box 114-D, Santiago Chile.
authors: N C
authors: NC
authors: Inestrosa
authors: H L
authors: HL
authors: Fernandez
authors: J
authors: J
authors: Garrido