Association study between BDNF gene polymorphisms and autism by three-dimensional gel-based microarray.
Single nucleotide polymorphisms (SNPs) are important markers which can be used in association studies searching for susceptible genes of complex diseases. High-throughput methods are needed for SNP genotyping in a large number of samples. In this study, we applied polyacrylamide gel-based microarray combined with dual-color hybridization for association study of four BDNF polymorphisms with autism. All the SNPs in both patients and controls could be analyzed quickly and correctly. Among four SNPs, only C270T polymorphism showed significant differences in the frequency of the allele (chi(2) = 7.809, p = 0.005) and genotype (chi(2) = 7.800, p = 0.020). In the haplotype association analysis, there was significant difference in global haplotype distribution between the groups (chi(2) = 28.19, p = 3.44e-005). We suggest that BDNF has a possible role in the pathogenesis of autism. The study also show that the polyacrylamide gel-based microarray combined with dual-color hybridization is a rapid, simple and high-throughput method for SNPs genotyping, and can be used for association study of susceptible gene with disorders in large samples.
Cheng
Lu
L
State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
Ge
Qinyu
Q
Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China.
Xiao
Pengfeng
P
State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
Sun
Beili
B
State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
Ke
Xiaoyan
X
Child Mental Health Research Center of Nanjing Brain Hospital affiliated of Nanjing Medical University, Nanjing, 210029, China.
Bai
Yunfei
Y
State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
Lu
Zuhong
Z
State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.|Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China.
D000483:Alleles; D001321:Autistic Disorder / Q000235:genetics*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
D019208:Brain-Derived Neurotrophic Factor; D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3