Effects of Madagascar yam extracts, Dioscorea antaly, on embryo-larval development of medaka fish, Oryzias latipes.
The yams edible starchy tubers, are of cultural, economic and nutritional importance in tropical and subtropical regions. The present study concerns the analysis at different levels of Dioscorea antaly toxicity to medaka embryo-larval development. The incubation of medaka fish embryos in a medium containing Dioscorea antaly extract resulted in a dose dependent reduction in survival rate. Survival rates were reduced up to 100% with extract concentrations of 4mg mL(-1). The LD(50) was estimated to be 0.86mg mL(-1)Dioscorea antaly. Anatomopathological studies did not show any caustic effects, irritation to mouth, throat or intestinal tract in surviving embryos but rather an inflammatory reaction in the liver. The data presented in this paper thus extends the use of medaka embryos as a valuable model to analyze the effects of food toxins.
Rakotobe
Lolona
L
FRE 3206 CNRS-USM 0505 Molécules de Communication et Adaptation des Micro-organismes, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris cedex 05, France.
Berkal
Miassa
M
Huet
Hélène
H
Djediat
Chakib
C
Jeannoda
Victor
V
Bodo
Bernard
B
Mambu
Lengo
L
Crespeau
François
F
Edery
Marc
M
D000818:Animals; D027742:Dioscorea / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007928:Lethal Dose 50; D008099:Liver / Q000473:pathology*; D008270:Madagascar; D023421:Models, Animal; D009928:Organ Specificity; D009990:Oryzias / Q000002:abnormalities / Q000196:embryology / Q000254:growth & development*; D010936:Plant Extracts / Q000633:toxicity*; D035281:Plant Tubers / Q000737:chemistry*; D010947:Plants, Toxic
D010936:Plant Extracts