title: VEGFR-1 regulates adult olfactory bulb neurogenesis and migration of neural progenitors in the rostral migratory stream in vivo.
abstract: The generation of new neurons in the olfactory bulb (OB) persists into adulthood and is a multistep process that includes proliferation, fate choice, migration, survival, and differentiation. Neural precursor cells destined to form olfactory interneurons arise in the subventricular zone (SVZ) and migrate along the rostral migratory stream (RMS) to the OB. Recently, some factors classically known from their effects on the vascular system have been found to influence different steps of adult neurogenesis. In the present study, we report a modulatory function for the vascular endothelial growth factor receptor-1 (VEGFR-1) in adult olfactory neurogenesis. We identified expression of VEGFR-1 in GFAP-positive cells within regions involved in neurogenesis of the adult mouse brain. To determine functions for VEGFR-1 in adult neurogenesis, we compared neural progenitor cell proliferation, migration, and differentiation from wild-type and VEGFR-1 signaling-deficient mice (Flt-1TK(-/-) mice). Our data show that VEGFR-1 signaling is involved in the regulation of proliferation of neuronal progenitor cells within the SVZ, migration along the RMS, and in neuronal differentiation and anatomical composition of interneuron subtypes within the OB. RMS migration in Flt-1TK(-/-) mice was altered mainly as a result of increased levels of its ligand VEGF-A, which results in an increased phosphorylation of VEGFR-2 in neuronal progenitor cells within the SVZ and the RMS. This study reveals that proper RMS migration is dependent on endogenous VEGF-A protein.
authors: Goethe University Medical School, Institute of Neurology (Edinger Institute), Neuroscience Center, 60528 Frankfurt am Main, Germany.
authors: Ina M
authors: IM
authors: Wittko
authors: Anne
authors: A
authors: Schänzer
authors: Andrey
authors: A
authors: Kuzmichev
authors: Fabian T
authors: FT
authors: Schneider
authors: Masabumi
authors: M
authors: Shibuya
authors: Sabine
authors: S
authors: Raab
authors: Karl H
authors: KH
authors: Plate
mesh_terms: D000367:Age Factors; D000818:Animals; D002464:Cell Migration Inhibition / Q000235:genetics / Q000502:physiology*; D006131:Growth Inhibitors / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology*; D019899:Paracrine Communication / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D006131:Growth Inhibitors; D040281:Vascular Endothelial Growth Factor Receptor-1