Optimization of solid-phase extraction using developed modern sorbent for trace determination of ametryn in environmental matrices.
In recent years, there has been a significant increase in molecularly imprinted solid-phase extraction (MISPE) technique for the purification and clean-up of environmental samples. In this study, solid-phase extraction using the imprinted polymer has been optimized with the experimental design approach for a triazine herbicide, named ametryn with regard to the critical factors such as sample pH, sample concentration, sample flow-rate, sample volume, elution solvent, washing solvent and sorbent mass. These factors were evaluated statistically and also validated with spiked drinking water samples and showed a good reproducibility over six consecutive days as well as six within-day experiments. Also, in order to the evaluate efficiency of the optimized MISPE protocols, enrichment capacity, reusability and cross-reactivity of cartridges have been studied. Finally, a selective MISPE was successfully demonstrated for ametryn with a recovery of above 90% for spiked drinking water samples. It was concluded that the central composite design could prove beneficial for aiding the MIP and MISPE development.
Koohpaei
A R
AR
Department of Occupational Health, Qom University of Medical Sciences, P.O. Box 3756, Qom 37185, Iran.
Shahtaheri
S J
SJ
Ganjali
M R
MR
Forushani
A Rahimi
AR
Golbabaei
F
F
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis*; D014881:Water Supply / Q000032:analysis
D004785:Environmental Pollutants; D006540:Herbicides; D011108:Polymers; D014227:Triazines; C100057:ametryne