Expression of two metallothionein genes in different brain regions of common carp.
The expression pattern of two metallothionein (MT) genes in response to temperature shock and exposure to Cd(2+) was investigated in the brain of common carp ( Cyprinus carpio ), in whole-animal experiments. The changes in the levels of MT-1 and MT-2 mRNA in the olfactory lobe, midbrain and cerebellum were followed by semiquantitative RT-PCR. The inducibility of the two MT genes was brain region and stressor-specific. Cd(2+) affected mostly the expression of MT-2, while the level of the MT-1 transcript did not change significantly in any of the brain regions examined. Moreover, the MT-2 expression was regulated spatially; MT-2 was induced significantly more strongly in the olfactory lobe than in the cerebellum or midbrain. A sudden temperature drop mainly affected the expression of the MT-1 gene; after 5 h of cold shock, the MT-1 mRNA level was about 25% of the basal value in the cerebellum and the midbrain region. The MT-2 expression did not change significantly during this treatment.
Ali
K Said
KS
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Szeged, P.O.Box 533, H-6701 Szeged, Hungary.
Ferencz
Ágnes
Á
Deér
Aranka K
AK
Nemcsók
J
J
Hermesz
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D000818:Animals; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D002104:Cadmium / Q000494:pharmacology; D002347:Carps; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D008668:Metallothionein / Q000096:biosynthesis*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D013997:Time Factors; D014018:Tissue Distribution
D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D002104:Cadmium; D008668:Metallothionein