Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.
Busche
Alena E L
AE
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance and Cluster of Excellence Frankfurt Macromolecular Complexes (CEF), University of Frankfurt, 60438 Frankfurt, Germany.
Aranko
A Sesilja
AS
Talebzadeh-Farooji
Mehdi
M
Bernhard
Frank
F
Dötsch
Volker
V
Iwaï
Hideo
H
D000595:Amino Acid Sequence; D004258:DNA Polymerase III / Q000737:chemistry*; D047668:Inteins; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D017510:Protein Folding; D019154:Protein Splicing; D017434:Protein Structure, Tertiary
C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III