Quercetin protects embryonic chicken spermatogonial cells from oxidative damage intoxicated with 3-methyl-4-nitrophenol in primary culture.
Diesel exhaust particles (DEP) are considered to be one of the most important air pollutants. In this study, the protective effect of quercetin, an antioxidant flavonoid, on oxidative damage of testicular cells was studied by analysis of the intracellular antioxidant system of embryonic chickens after treatment with 3-methyl-4-nitrophenol (PNMC) derived from DEP. Testicular cells from 18-day-old embryos were cultured in serum-free McCoys'5A medium and challenged with PNMC (10(-7) to 10(-5)M) alone or in combinations with quercetin (1.0 microg/ml) for 48h. Results showed that exposure to PNMC (10(-5)M) induced condensed nuclei and vacuolated cytoplasm, a decrease in testicular cell viability and spermatogonial cell number. Exposure to PNMC induced lipid peroxidation by an elevation of thiobarbituric acid reactive substances as well as decreasing glutathione peroxidation activity and superoxide dismutase activity. However, simultaneous supplementation with quercetin restored these parameters to the similar levels as the control. PNMC is therefore concluded to have induced the oxidative stress of the spermatogonial cells, which can be attenuated by combined quercetin treatment. Our results support the therapeutic use of quercetin in the prevention or treatment of the reproductive toxicity by environmental toxicant PNMC.
Mi
Yuling
Y
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Zhang
Caiqiao
C
Li
ChunMei
C
Taneda
Shinji
S
Watanabe
Gen
G
Suzuki
Akira K
AK
Taya
Kazuyoshi
K
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002452:Cell Count; D002642:Chick Embryo; D003408:Cresols / Q000633:toxicity*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D052638:Particulate Matter / Q000633:toxicity; D011794:Quercetin / Q000494:pharmacology*; D013093:Spermatogonia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D001335:Vehicle Emissions / Q000633:toxicity
D000393:Air Pollutants; D000975:Antioxidants; D003408:Cresols; D052638:Particulate Matter; D001335:Vehicle Emissions; C008636:4-nitro-3-cresol; D011794:Quercetin