title: The effect of tissue culture on suture holding strength and degradation in canine tendon.
abstract: The purpose of this study was to assess tendon metabolism and suture pull-out strength after simple tendon suture in a tissue culture model. One hundred and twelve flexor digitorum profundus tendons from 28 dogs were cultured for 7, 14, or 21 days with or without a static tensile load. In both groups increased levels of matrix metalloproteinase (MMP) mRNA was noted. Suture pull-out strength did not decrease during tissue culture. While the presence of a static load had no effect on the pull-out strength, it did affect MMP mRNA expression. This tissue culture model could be useful in studying the effect of factors on the tendon-suture interface.
authors: Orthopedic Biomechanics Laboratory, Mayo Clinic Rochester, MN 55905, USA.
authors: H
authors: H
authors: Omae
authors: C
authors: C
authors: Zhao
authors: Y-L
authors: YL
authors: Sun
authors: M E
authors: ME
authors: Zobitz
authors: S L
authors: SL
authors: Moran
authors: P C
authors: PC
authors: Amadio
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D004285:Dogs; D005552:Forelimb; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013537:Sutures; D013710:Tendons / Q000378:metabolism* / Q000503:physiopathology* / Q000601:surgery; D013718:Tensile Strength; D046509:Tissue Culture Techniques; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism; D016474:Weight-Bearing
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases