Vibrio hangzhouensis sp. nov., isolated from sediment of the East China Sea.
Strain CN83T, a Gram-negative, aerobic, rod-shaped bacterium, was isolated from sediment of the East China Sea. The isolate was catalase- and oxidase-positive and cells were motile by means of polar flagella. The DNA G+C content was 44.9 mol%. The major fatty acids were C16:1omega7c and/or iso-C15:0 2-OH, C16:0, C18:1omega7c, C14:0 and C12:0. 16S rRNA gene sequence analysis showed that strain CN83T belonged to the genus Vibrio and had the highest sequence similarity to Vibrio agarivorans (98.4%) and Vibrio campbellii (97.8%). Phylogenetic analysis revealed that strain CN83T formed a monophyletic clade adjacent to the type strain of V. agarivorans. The DNA-DNA hybridization values of strain CN83T with V. agarivorans DSM 13756T and V. campbellii DSM 19270T were 44.6 and 25.5%, respectively. On the basis of the phenotypic and genotypic data, strain CN83T represents a novel species of the genus Vibrio, for which the name Vibrio hangzhouensis sp. nov. is proposed. The type strain is CN83T (=CGMCC 1.7062T=JCM 15146T).
Xu
Xue-Wei
XW
Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, PR China. xuxw@sio.org.cn
Wu
Yue-Hong
YH
Wang
Chun-Sheng
CS
Oren
Aharon
A
Wu
Min
M
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008124:Locomotion; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase