title: Label-free and amplified quantitation of proteins in complex mixtures using diffractive optics technology.
abstract: Immunoaffinity approaches remain invaluable tools for characterization and quantitation of biopolymers. Their application in separation science is often limited due to the challenges of immunoassay development. Typical end-point immunoassays require time consuming and labor-intensive approaches for optimization. Real-time label-free analysis using diffractive optics technology (dot) helps guide a very effective iterative process for rapid immunoassay development. Both label-free and amplified approaches can be used throughout feasibility testing and ultimately in the final assay, providing a robust platform for biopolymer analysis over a very broad dynamic range. We demonstrate the use of dot in rapidly developing assays for quantitating (1) human IgG in complex media, (2) a fusion protein in production media and (3) protein A contamination in purified immunoglobulin preparations.
authors: TouchDown Biomarketing BV, Bemmel, The Netherlands.
authors: Steve
authors: S
authors: Cleverley
authors: Irene
authors: I
authors: Chen
authors: Jean-François
authors: JF
authors: Houle
mesh_terms: D001704:Biopolymers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002138:Calibration; D045424:Complex Mixtures / Q000737:chemistry; D004867:Equipment Design; D017743:Equipment Reuse; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D013205:Staphylococcal Protein A / Q000032:analysis
chemical_list: D001704:Biopolymers; D045424:Complex Mixtures; D007074:Immunoglobulin G; D011506:Proteins; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A