title: IL-13 expression by blood T cells and not eosinophils is increased in asthma compared to non-asthmatic eosinophilic bronchitis.
abstract: BACKGROUND
  In asthma interleukin (IL)-13 is increased in the airway compared with non-asthmatic eosinophilic bronchitis. Whether this differential expression is specific to the airway or is more generalised is uncertain.
  
  
  METHODS
  We sought to examine IL-13 expression in peripheral blood T-cells and eosinophils in asthma and non-asthmatic eosinophilic bronchitis. Peripheral blood CD3+ cell and eosinophil intracellular IL-13 expression from subjects with asthma, non-asthmatic eosinophilic bronchitis and healthy controls was assessed. The effect of priming by asthmatic serum on the release of IL-13 by peripheral blood mononuclear cells from healthy subjects was examined and the serum from these subjects was analysed for a range of chemokines and cytokines.
  
  
  RESULTS
  The median (IQR)% intracellular IL-13 expression by CD3+ cells was increased in asthma [5.3 (2.7-9.8)%; n = 12] compared to non-asthmatic eosinophilic bronchitis [1.1 (0.5-3)%; n = 7] and healthy controls [1.7 (0.2-3%); n = 9] (p = 0.02), but was not significantly different in eosinophils across the groups. IL-13 released from healthy peripheral blood mononuclear cells (n = 10) was increased by asthmatic serum [117 (47.8-198)pg/ml] compared to control [78.5 (42.6-128)pg/ml; p = 0.02), but was not affected by non-asthmatic serum.
  
  
  CONCLUSIONS
  Our findings support the view that IL-13 expression is increased in peripheral blood-derived T cells in asthma and that asthmatic serum up-regulates IL-13 release from healthy peripheral blood mononuclear cells.
authors: Institute of Lung Health, University of Leicester, Leicester, UK. salman95@yahoo.com
authors: Salman
authors: S
authors: Siddiqui
authors: Glenn
authors: G
authors: Cruse
authors: Susan
authors: S
authors: McKenna
authors: William
authors: W
authors: Monteiro
authors: Vijay
authors: V
authors: Mistry
authors: Andrew
authors: A
authors: Wardlaw
authors: Christopher
authors: C
authors: Brightling
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood* / Q000473:pathology; D001991:Bronchitis / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D004804:Eosinophils / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D018793:Interleukin-13 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D018793:Interleukin-13