Panel reactive antibody in thalassemic serum inhibits proliferation and differentiation of cord blood CD34+ cells in vitro.
Thalassemic children are at a high risk of graft rejection in cord blood transplantation. To investigate this possible mechanism, the authors evaluated the effect of panel reactive antibody on the growth of CD34(+) cells in vitro. On semisolid medium, CD34(+) cells derived from cord blood were incubated with thalassemic serum, and colony-forming units were counted after 10 days of culture. After incubation with serum-specific panel reactive antibody, profound decreases were found in the numbers of CFU-GM, CFU-GEMM and BFU-E compared with controls. The results indicated that serum-specific panel reactive antibody might have an apparent inhibition effect on proliferation and differentiation of cord blood CD34(+) cells.
Fang
Jian-Pei
JP
Department of Pediatrics, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. jpfang2005@163.com
Xu
Lu-Hong
LH
Yang
Xing-Ge
XG
Wu
Yan-Feng
YF
Weng
Wen-Jun
WJ
Xu
Hong-Gui
HG
D000906:Antibodies / Q000097:blood / Q000276:immunology*; D018952:Antigens, CD34 / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000276:immunology*; D017086:beta-Thalassemia / Q000097:blood / Q000276:immunology*
D000906:Antibodies; D018952:Antigens, CD34