Localization and characterization of 170 BAC-derived clones and mapping of 94 microsatellites in the Hessian fly.
Ninety-four microsatellites from enriched genomic libraries of Hessian fly (Hf, Mayetiola destructor [Say]) were localized to 170 cognate clones in an Hf bacterial artificial chromosome (BAC) library. These microsatellite-positive BAC clones were physically mapped to polytene chromosomes by fluorescent in situ hybridization. The mapped microsatellite loci can be used to study the genetic diversity and population structure of Hf.
Schemerhorn
Brandon J
BJ
USDA-ARS, Department of Entomology, Purdue University, West Lafayette, Indiana 47907, USA. bschemer@purdue.edu
Crane
Yan M
YM
Morton
Philip K
PK
Aggarwal
Rajat
R
Benatti
Thiago
T
D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D019295:Computational Biology; D004175:Diptera / Q000235:genetics*; D015723:Gene Library; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D017422:Sequence Analysis, DNA