hTERT promotes the invasion of telomerase-negative tumor cells in vitro.
Telomerase activation is a common feature of most types of human cancers. Although several studies have shown that activation of telomerase might participate in the progression of tumors, the mechanism by which telomerase activation causes the invasion and metastasis of tumors remains unclear. In this study, we transfected a vector containing the full-length cDNA of hTERT into a telomerase-negative osteosarcoma cell line U2OS (hTERT/U2OS). Vacant vector-transfected U2OS cells served as a control (EGFP/U2OS). We then compared the biological and vitodynamic changes in these transfected and untransfected U2OS cells. The hTERT protein was detected in hTERT/U2OS cells by Western blot analysis and immunochemistry assay. The telomere length in hTERT/U2OS cells was longer than that in EGFP/U2OS and untransfected U2OS cells. We also found using vacuum micropipette aspiration that hTERT transfection did not only promote the proliferation of hTERT-transfected U2OS cells but also increased the cellular adhesion capacity to the extracellular matrix. Transwell matrigel assay confirmed an increased invasion ability in hTERT/U2OS cells. These results strongly suggest that hTERT transfection promotes the invasion of telomerase-negative cells. Telomerase-mediated telomere maintenance enables these cells to achieve a fully malignant endpoint, including invasion and metastasis.
Yu
Song-Tao
ST
Institute of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
Chen
Ling
L
Wang
Hong-Jin
HJ
Tang
Xu-Dong
XD
Fang
Dian-Chun
DC
Yang
Shi-Ming
SM
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction; D019098:Telomerase / Q000502:physiology*; D016615:Telomere; D014162:Transfection
C509186:TERT protein, human; D019098:Telomerase