title: Novel separation medium spongy monolith for high throughput analyses.
abstract: Sponge-like material was utilized as novel chromatographic media for high throughput analyses. The pore size of the sponge-like material was several dozen micrometer, and was named spongy monolith because it consists of continuous structured copolymers, which was made of poly(ethylene-co-vinyl acetate), such as monolithic materials including silica monoliths and organic polymer monoliths. The spongy monolith was packed into a stainless steel column (100 mm x 4.6 mm I.D.) and evaluated in liquid chromatography (LC) with an on-line column-switching LC concentration system. The results indicate that the packed column could be used with high flow rates and low back pressure (9.0 mL/min at 0.5 MPa). Furthermore, bisphenol A was quantitatively recovered by on-line column-switching LC concentration with the spongy monolithic column. Additionally, the adsorption capacity and physical strength of the media was enhanced via chemical modification of spongy monoliths using glycerol dimethacrylate. The results compared with original spongy monolith demonstrated that a higher adsorption capacity was achieved on a shorter column, and a stable low back pressure was obtained at high throughput elution even with a longer column.
authors: Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki, Aobaku, Sendai 9808579, Japan.
authors: Fuminori
authors: F
authors: Watanabe
authors: Takuya
authors: T
authors: Kubo
authors: Kunimitsu
authors: K
authors: Kaya
authors: Ken
authors: K
authors: Hosoya
mesh_terms: D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; D011108:Polymers; D011145:Polyvinyls; D012822:Silicon Dioxide; C016438:ethylenevinylacetate copolymer; C006780:bisphenol A; D000098826:Bisphenol A Compounds