Hepatocellular carcinoma cells deteriorate the biophysical properties of dendritic cells.
Dendritic cells (DCs) are potent antigen-presenting cells and induce antigen-specific immune responses in the organism. The dysfunction of DCs has been implicated in tumor-bearing host. In order to elucidate the effects of tumor microenvironment on the functions of DCs from interdisciplinary aspects, we characterized the biophysical properties of DCs co-cultured with hepatocellular carcinoma cells (HCC). The results showed that the biophysical characteristics of immature and mature DCs were severely impaired by HCC compared with those under normal conditions, including the increased osmotic fragilities, decreased cell membrane fluidities, increased membrane viscoelastic properties, dysfunction and increased expression of cytoskeleton protein F-actin, as well as the deteriorated transendothelium migration. The impaired biophysical properties of DCs may be one of many aspects of the immune escape mechanisms of tumors. These results are clinically and instructionally significant with regard to how to enhance efficiency of the anti-tumor therapy based on DCs.
Zeng
Zhu
Z
Department of Biophysics, Health Science Center, Peking University, Beijing 100191, People's Republic of China.
Yao
Weijuan
W
Xu
Xiaofeng
X
Xu
Guoqiang
G
Long
Jinhua
J
Wang
Xianwei
X
Wen
Zongyao
Z
Chien
Shu
S
D000199:Actins / Q000378:metabolism; D055592:Biophysical Phenomena; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000628:therapy; D002465:Cell Movement; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D042783:Endothelial Cells / Q000166:cytology; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology* / Q000628:therapy; D019139:Tumor Escape
D000199:Actins