[Identification of active efflux system gene qacA/B in methicillin-resistant Staphylococcus aureus and its significance].
OBJECTIVE
To detect the active efflux gene qac gene in methicillin-resistant Staphylococcus aureus (MRSA) by hem-nested polymerase chain reaction (PCR) and to learn the carrier condition of qac gene.


METHODS
The active efflux gene qacA/B and qacB of 80 strains MRSA isolated from clinical specimens from Aug 2006 to March 2008 were amplified in vitro by hem-nested PCR with the primers designed by computers based on qac information of Genbank, and the PCR fragments were sequenced and analyzed.


RESULTS
We detected qacA/B in 19 out of the 80 MRSA strains (23.75%) and qacB in 18 out of the 80 MRSA strains (22.5%). Compared with sequences of qacA (NO.X56628) and qacB(NO.AF535087) in the Genbank, 98% and 97% were identical, respectively.


CONCLUSIONS
The active efflux gene qac gene in MRSA is detected by hem-nested PCR. The proportion of qac gene positive strains is high in clinical practice, which is related to its multi-antibiotic resistance.
Zheng
Rong
R
Department of Clinical Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Wang
Min
M
He
Bin
B
Li
Xianping
X
Cao
Hong
H
Liang
Hao
H
Qing
Zhiju
Z
Tang
Aiguo
A
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D018432:Drug Resistance, Multiple / Q000235:genetics; D026901:Membrane Transport Proteins / Q000032:analysis / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C099272:QacB protein, Staphylococcus aureus; C068498:qacA protein, Staphylococcus aureus