title: Statistical recoupling prior to significance testing in nuclear magnetic resonance based metabonomics.
abstract: Significance testing is a crucial step in metabolic biomarker recovery from the metabolome-wide latent variables computed by multivariate statistical analysis. In this study we propose an algorithm based on the landscape of the covariance/correlation ratio of consecutive variables along the chemical shift axis to restore, prior to significance testing, the spectral dependency and recouple variables in clusters which correspond to physical, chemical, and biological entities: statistical recoupling of variables (SRV). Variables are associated into a series of clusters, which are then considered as individual objects for the control of the false discovery rate. Compared to classical procedures, it is found that SRV allows efficient recovery of statistically significant metabolic variables. The proposed SRV method when associated with the Benjamini-Yekutieli correction retains a low level of significant variables in the noise areas of the nuclear magnetic resonance (NMR) spectrum, close to that observed using the conservative Bonferroni correction (false positive rate), while also allowing successful identification of statistically significant metabolic NMR signals in cases where the classical procedures of Benjamini-Yekutieli and Benjamini-Hochberg (false discovery rate) fail. This procedure improves the interpretability of latent variables for metabolic biomarker recovery.
authors: Université de Lyon, Centre de RMN à Très Hauts Champs, CNRS/ENS Lyon/UCB Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
authors: Benjamin J
authors: BJ
authors: Blaise
authors: Laetitia
authors: L
authors: Shintu
authors: Bénédicte
authors: B
authors: Elena
authors: Lyndon
authors: L
authors: Emsley
authors: Marc-Emmanuel
authors: ME
authors: Dumas
authors: Pierre
authors: P
authors: Toulhoat
mesh_terms: D000465:Algorithms; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D005189:False Positive Reactions; D008279:Magnetic Resonance Imaging; D055432:Metabolomics; D015233:Models, Statistical
chemical_list: D029742:Caenorhabditis elegans Proteins