title: Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep.
abstract: Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep.
authors: Embrapa Gado de Corte, BR 262, km 4, CP 154, CEP 79002-970 Campo Grande - MS, Brazil. andreott@cnpgc.embrapa.br
authors: Renato
authors: R
authors: Andreotti
authors: Maria de Fátima Cepa
authors: Mde F
authors: Matos
authors: Kelly Noda
authors: KN
authors: Gonçalves
authors: Leandra Marla
authors: LM
authors: Oshiro
authors: Manoel Sebastião da Costa
authors: MS
authors: Lima-Junior
authors: Fernando
authors: F
authors: Paiva
authors: Fábio Leivas
authors: FL
authors: Leite
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D018688:Neospora / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D012756:Sheep / Q000097:blood*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; C115389:SRS2 protein, Neospora caninum