title: Inter-laboratory evaluation of the bioluminescent Salmonella reverse mutation assay using 10 model chemicals.
abstract: We have developed the bioluminescent Salmonella reverse mutation assay as a tool for detecting mutagenicity applicable for high-throughput screening of new chemicals. In this study, we report the inter-laboratory evaluation of the assay using 10 model chemicals in five independent laboratories located in the USA (Groton, CT; Cambridge, MA and La Jolla, CA), Europe (Sandwich, Kent, UK) and Asia (Nagoya, Japan). The studies were performed in blinded fashion in all sites except for Groton and Cambridge laboratories. The chemicals were tested in at least three independent experiments using strains TA98-lux and TA100-lux in the presence and absence of metabolic activation. The results were statistically evaluated and compared to published results. Seven of the 10 compounds were positive in either TA98-lux and/or TA100-lux in the presence or absence of metabolic activation. The positive compound set included: nitrofurazone, 3-3'-dimethoxybenzidine, benzo[a]pyrene, 1,4-benzoquinone dioxime, 2-amino-5-nitrophenol, 2-bromo-4,6-dinitroaniline and busulfan. The remaining three compounds, namely, anthracene, crystal violet and benzyl chloride were negative in both Salmonella strains. Final results for individual compounds yielded 100% agreement among the laboratories and published data. Detailed comparison of all 40 individual test conditions yielded 93% (37 of 40) agreement among participating laboratories. We conclude that the bioluminescent Salmonella reverse mutation assay is a robust, accurate and economical higher throughput assay applicable for the mutagenicity screening of chemicals.
authors: Pfizer Global Research and Development, Drug Safety Research and Development, Eastern Point Road, Groton, CT 06340, USA. joel.i.ackerman@pfizer.com
authors: Joel
authors: J
authors: Ackerman
authors: Rahul
authors: R
authors: Sharma
authors: Jonathan
authors: J
authors: Hitchcock
authors: Toshiaki
authors: T
authors: Hayashi
authors: Yoshikazu
authors: Y
authors: Nagai
authors: Shuanglian
authors: S
authors: Li
authors: Shuyan
authors: S
authors: Lu
authors: Juan
authors: J
authors: Miret
authors: Kim
authors: K
authors: Tang
authors: Fiona
authors: F
authors: Spence
authors: Jiri
authors: J
authors: Aubrecht
mesh_terms: D007753:Laboratories; D008163:Luminescent Measurements; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects* / Q000235:genetics*; D012475:Salmonella / Q000187:drug effects* / Q000235:genetics*
chemical_list: D009153:Mutagens