Overexpression of Annexin II affects the proliferation, apoptosis, invasion and production of proangiogenic factors in multiple myeloma.
The abnormal expression of Annexin II (AnxA2, A2) has been associated with the development of tumors; however, its expression and function in multiple myeloma (MM) is less known. We compared the expression of AnxA2 in primary myeloma cells from MM patients with that in normal plasma cells from normal subjects and found that myeloma cells from patients had higher expression of AnxA2. Expression of AnxA2 was also significantly higher in MM cell lines U266 and RPMI8226, compared with other hematologic tumor cell lines. Transfecting U266 and RPMI8226 cells with the small interfering RNA (siRNA) that targets human AnxA2 led to significant downregulation of AnxA2 expression, which resulted in the decreased proliferation, invasive potential and increased apoptosis of U266 and RPMI8226 cell lines. Silencing AnxA2 gene by siRNA also inhibited the expression of pro-angiogenic molecules including VEGF-C, VEGF-R2, MMP-2, MMP-9, MT1-MMP and TIMP-2 in the two cell lines. Our data suggested that the AnxA2 is overexpressed in MM patients and myeloma cell lines U266 and RPMI8226, and that AnxA2 overexpression appeared to affect the proliferation, apoptosis, invasive potential and production of pro-angiogenic factors in MM cell lines U266 and RPMI8226.
Bao
Hongyu
H
Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 708 Renmin Road, Suzhou, 215007, China.|Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou, China.
Jiang
Miao
M
Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 708 Renmin Road, Suzhou, 215007, China.|Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou, China.
Zhu
Mingqing
M
Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 708 Renmin Road, Suzhou, 215007, China.|Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou, China.
Sheng
Fei
F
Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 708 Renmin Road, Suzhou, 215007, China.|Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou, China.
Ruan
Jia
J
Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medical College and New York-Presbyterian Hospital, New York, USA.
Ruan
Changgeng
C
Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 708 Renmin Road, Suzhou, 215007, China. changgengruan@hotmail.com.
D017306:Annexin A2 / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D010950:Plasma Cells / Q000473:pathology* / Q000502:physiology; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017306:Annexin A2; D034741:RNA, Small Interfering