title: Evaluation of the efficacy of platelet-rich plasma in delivering BMSCs into 3D porous scaffolds.
abstract: Platelet-rich plasma (PRP), derived from autologous whole blood, is rich in fibrinogen and platelets, which release multiple active factors in response to thrombin. The purpose of this study was to evaluate the efficacy of PRP in delivering bone marrow stromal cells (BMSCs) into 3D porous scaffolds in comparison with platelet-poor plasma (PPP) and Dulbecco's modified Eagle's medium (DMEM). The seeding efficacy, proliferation, and differentiation of the BMSCs seeded into poly(lactic-co-glycolic acid) (PLGA) porous scaffolds via PRP, PPP, and DMEM were tested. Additionally, BMSC/PLGA constructs were implanted in goat calvarial defects to radiologically and histologically detect the capability of PRP for enhancing bone regeneration. The results showed that the BMSC seeding efficacy of PRP was similar as that of PPP, and greater potential for in vitro BMSC proliferation and in vivo bone regeneration than PPP and DMEM. Therefore, PRP can potentially accomplish convenient and effective cell delivery into porous scaffolds, which is valuable in bone tissue engineering.
authors: Department Six of Plastic Surgery Hospital, Peking Union Medical College, China Academy of Medical Sciences, Shijingshan District, Beijing, China.
authors: Hua
authors: H
authors: Lei
authors: Department Six of Plastic Surgery Hospital, Peking Union Medical College, China Academy of Medical Sciences, Shijingshan District, Beijing, China.
authors: Ran
authors: R
authors: Xiao
authors: Department Six of Plastic Surgery Hospital, Peking Union Medical College, China Academy of Medical Sciences, Shijingshan District, Beijing, China.
authors: Xiao Jun
authors: XJ
authors: Tang
authors: Department Six of Plastic Surgery Hospital, Peking Union Medical College, China Academy of Medical Sciences, Shijingshan District, Beijing, China.
authors: Lai
authors: L
authors: Gui
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D001842:Bone and Bones / Q000166:cytology / Q000000981:diagnostic imaging; D002454:Cell Differentiation; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods*; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification; D015870:Gene Expression; D006041:Goats; D019344:Lactic Acid; D010012:Osteogenesis / Q000235:genetics; D053657:Platelet-Rich Plasma; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D019736:Prostheses and Implants; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells; D054457:Tissue Scaffolds; D014057:Tomography, X-Ray Computed
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA; D000469:Alkaline Phosphatase