title: Interference of Dermatophagoides' specific immunoglobulins G in the quantification of mite's specific immunoglobulins E.
abstract: In order to investigate the interference of specific IgG in the quantification of specific IgE, using the ImmunoCAP 250 system, we studied, in parallel, the interference by total adsorption of interferent and the classical method of adding interferent increasingly. Furthermore, to evaluate if the interference is affected by different solid phases, total extract of Dermatophagoides pteronyssinus and recombinant allergens of Dermatophagoides farinae were used. The results showed a statistical significant interference by IgG in the quantification of the specific IgE, but neither analytical nor clinical significant interference were observed. Therefore, this analytical system provides an accurate method for determination of the specific IgE concentration contributing to the allergic disease diagnosis quality.
authors: Laboratório de Patologia Clinica, Hospital Sousa Martins, Guarda, Portugal.
authors: Patricia
authors: P
authors: Fonseca
authors: Paulo
authors: P
authors: Tavares-Ratado
authors: Cândida T
authors: CT
authors: Tomaz
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D040001:Dermatophagoides farinae / Q000276:immunology*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D003951:Diagnostic Errors; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D039741:Antigens, Dermatophagoides; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E