Isolation of mesenchymal stem cells from human placental decidua basalis and resistance to hypoxia and serum deprivation.
Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy and tissue engineering, which can be isolated from various sources of human adult tissues such as bone marrow and adipose tissue. However, cells from these tissues must be obtained through invasive procedures and sometimes the individual difference is hard to control. Hence, the search continues for an ethically conducive, easily accessible and controllable source of stem cells. We herein report the isolation of a population of stem cells from the human placental decidua basalis (termed as PDB-MSCs), a maternal portion of placenta. PDB-MSCs were further shown to express markers common to MSCs and positive for SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and Oct-4. In order to facilitate the further utility in ischemic diseases, we tested the apoptosis of PDB-MSCs in hypoxia and serum deprivation, two components of ischemia in vivo. Taken together, our findings indicate that PDB-MSCs are resistant to hypoxia and serum deprivation, which may relate to Bcl-2.
Huang
Yong-Can
YC
Division of Stem cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.
Yang
Zhi-Ming
ZM
Chen
Xiao-He
XH
Tan
Mei-Yun
MY
Wang
Jia
J
Li
Xiu-Qun
XQ
Xie
Hui-Qi
HQ
Deng
Li
L
D000273:Adipose Tissue / Q000166:cytology; D017209:Apoptosis; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D016895:Culture Media, Serum-Free / Q000378:metabolism; D003656:Decidua / Q000473:pathology*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D000860:Hypoxia; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010641:Phenotype; D010920:Placenta / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy
D016895:Culture Media, Serum-Free; D005819:Genetic Markers