title: Characterization of salivaricin CRL 1328, a two-peptide bacteriocin produced by Lactobacillus salivarius CRL 1328 isolated from the human vagina.
abstract: Salivaricin CRL 1328 is a heat-stable bacteriocin produced by Lactobacillus salivarius CRL 1328, a strain isolated from healthy human vagina, with potential applications for preventing urogenital infections. The objective of this study was to characterize the locus responsible for salivaricin CRL 1328 production and its mechanism of action against Enterococcus faecalis MP97 as the sensitive strain. Oligonucleotides were designed based on sequences of antimicrobial peptides previously described in the literature. The salivaricin CRL 1328 cluster was identified, sequenced and analyzed. This cluster was similar to the previously described ABP118 which codified for a two-peptide bacteriocin. The putative mature peptides of salivaricin CRL 1328, Salalpha and Salbeta were chemically synthesized. These peptides did not show bacteriocin activity when assayed individually. Both peptides exhibited optimal antimicrobial activity at an equimolar ratio. Spectroscopic fluorescence assays were carried out using the synthetic peptides to study the effect of salivaricin on proton motive force. This bacteriocin was shown to dissipate membrane potential and the transmembrane proton gradient, both components of proton motive force. E. faecalis MP97 cells treated with salivaricin CRL 1328 peptides were observed in transmission electron microscopy which revealed ultrastructural modifications of the cell wall.
authors: Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145 (T4000ILC), San Miguel de Tucumán, Tucumán, Argentina. estebanvera@cerela.org.ar
authors: Esteban
authors: E
authors: Vera Pingitore
authors: Elvira María
authors: EM
authors: Hébert
authors: María Elena
authors: ME
authors: Nader-Macías
authors: Fernando
authors: F
authors: Sesma
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D014621:Vagina / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C545224:salivaricin CRL 1328