IL-9 as a mediator of Th17-driven inflammatory disease.
We report that like other T cells cultured in the presence of transforming growth factor (TGF) beta, Th17 cells also produce interleukin (IL) 9. Th17 cells generated in vitro with IL-6 and TGF-beta as well as purified ex vivo Th17 cells both produced IL-9. To determine if IL-9 has functional consequences in Th17-mediated inflammatory disease, we evaluated the role of IL-9 in the development and progression of experimental autoimmune encephalomyelitis, a mouse model of multiple sclerosis. The data show that IL-9 neutralization and IL-9 receptor deficiency attenuates disease, and this correlates with decreases in Th17 cells and IL-6-producing macrophages in the central nervous system, as well as mast cell numbers in the regional lymph nodes. Collectively, these data implicate IL-9 as a Th17-derived cytokine that can contribute to inflammatory disease.
Nowak
Elizabeth C
EC
Department of Microbiology and Immunology, Dartmouth Medical School and the Norris Cotton Cancer Center, Lebanon, NH 03756, USA.
Weaver
Casey T
CT
Turner
Henrietta
H
Begum-Haque
Sakhina
S
Becher
Burkhard
B
Schreiner
Bettina
B
Coyle
Anthony J
AJ
Kasper
Lloyd H
LH
Noelle
Randolph J
RJ
D019264:Adoptive Transfer; D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology / Q000276:immunology / Q000473:pathology; D005260:Female; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000276:immunology* / Q000378:metabolism; D016906:Interleukin-9 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053651:Receptors, Interleukin-9 / Q000172:deficiency / Q000235:genetics / Q000276:immunology; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000276:immunology*
C508448:Il9r protein, mouse; D018836:Inflammation Mediators; D016906:Interleukin-9; D053651:Receptors, Interleukin-9