Porcine liver sinusoidal endothelial cells contribute significantly to intrahepatic ammonia metabolism.
Ammonia metabolism in the liver has been largely credited to hepatocytes (HCs). We have shown that liver nonparenchymal cells that include liver sinusoidal endothelial cells (LSECs) produce ammonia. To address the limited knowledge regarding a role for LSECs in ammonia metabolism, we investigated the ammonia metabolism of isolated LSECs and HCs under three different conditions: (1) bioreactors containing LSECs (LSEC-bioreactors), (2) bioreactors containing HCs (HC-bioreactors), and (3) separate bioreactors containing LSECs and HCs connected in sequence (Seq-bioreactors). Our results showed that LSEC-bioreactors released six-fold more ammonia (22.2 nM/hour/10(6) cells) into the growth media than HC-bioreactors (3.3 nM/hour/10(6) cells) and Seq-bioreactors (3.8 nM/hour/10(6) cells). The glutamate released by LSEC-bioreactors (32.0 nM/hour/10(6) cells) was over four-fold larger than that released by HC-bioreactors and Seq-bioreactors (<7 nM/hour/10(6) cells). LSEC-bioreactors and HC-bioreactors consumed large amounts of glutamine (>25 nM/hour/10(6) cells). Glutaminase is known for catalyzing glutamine into glutamate and ammonia. To determine if this mechanism may be responsible for the large levels of glutamate and ammonia found in LSEC-bioreactors, immunolabeling of glutaminase and messenger RNA expression were tested. Our results demonstrated that glutaminase was present with colocalization of an LSEC-specific functional probe in lysosomes of LSECs. Furthermore, using a nucleotide sequence specific for kidney-type glutaminase, reverse-transcription polymerase chain reaction revealed that this isoform of glutaminase was expressed in porcine LSECs.


CONCLUSIONS
LSECs released large amounts of ammonia, perhaps due to the presence of glutaminase in lysosomes. The ammonia and glutamate released by LSECs in Seq-bioreactors were used by hepatocytes, suggesting an intrahepatic collaboration between these two cell types.
Nedredal
Geir I
GI
Department of Digestive Surgery, University Hospital Northern Norway and Institute of Clinical Medicine, Norway.
Elvevold
Kjetil
K
Ytrebø
Lars M
LM
Fuskevåg
Ole-Martin
OM
Pettersen
Ingvild
I
McCourt
Peter A G
PA
Bertheussen
Kjell
K
Smedsrød
Bård
B
Revhaug
Arthur
A
D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D019149:Bioreactors; D042783:Endothelial Cells / Q000378:metabolism*; D018698:Glutamic Acid / Q000096:biosynthesis; D005972:Glutaminase / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D034421:Sus scrofa
D005973:Glutamine; D019344:Lactic Acid; D018698:Glutamic Acid; D000641:Ammonia; D005972:Glutaminase