Metal ion CHElate-aSSisted LIGAtion (CHESS LIGA) for SNP detection on microarrays.
We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.
Le
Thi Hien
TH
Chemistry Department, M.V. Lomonossov Moscow State University, Leninskije Gory, Moscow 119991, Russia.
Oretskaya
Tatiana S
TS
Zatsepin
Timofei S
TS
D001483:Base Sequence; D002614:Chelating Agents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D046208:Microchip Analytical Procedures / Q000379:methods*; D008969:Molecular Sequence Data; D009532:Nickel / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides; D020641:Polymorphism, Single Nucleotide; D012995:Solubility; D013763:Tetrahydrofolates / Q000737:chemistry
D002614:Chelating Agents; D015342:DNA Probes; D007477:Ions; D008670:Metals; D009841:Oligonucleotides; D013763:Tetrahydrofolates; C013123:5,10-methylenetetrahydrofolic acid; D009532:Nickel; D004247:DNA