Study on immunostimulating activity of macrophage treated with purified polysaccharides from liquid culture and fruiting body of Lentinus edodes.
Lentinus edodes is a well-known edible and medicinal mushroom used in Oriental cultures. Recently, L. edodes has attracted a lot of attention owing to its antifungal activity, antibacterial activity, antiviral activity, hepatoprotective effect, antitumor activities, and immunomodulatory and cytotoxic effects. In this study, the water-soluble crude polysaccharides, CPF and CPB, which were obtained from the fruiting body and culture cell-free broth of L. edodes by hot-water extraction and ethanol precipitation, were fractionated by DEAE cellulose and Sepharose CL-6B column chromatography, resulting in six polysaccharide fractions, CPFN-G-I, CPFN-G-II, CPFN-G-III, CPFA-G, CPBN-G, and CPBA-G. Among these fractions, CPFN-GI, CPBN-G, and CPBA-G were shown to stimulate the functional activation of macrophages including NO production cytokine expression and phagocytosis.
Lee
Hee Hwan
HH
Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200-701, Korea.
Lee
Jong Seok
JS
Cho
Jae Youl
JY
Kim
Young Eon
YE
Hong
Eock Kee
EK
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000276:immunology*; D007114:Immunization; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D011134:Polysaccharides / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D020942:Shiitake Mushrooms / Q000737:chemistry / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide