Hollow fiber-based liquid-phase microextraction (HF-LPME) of ibuprofen followed by FIA-chemiluminescence determination using the acidic permanganate-sulfite system.
Hollow fiber-based liquid-phase microextraction (HF-LPME) is a relatively new technique employed in analytical chemistry for sample pretreatment which offers more selectivity and sensitivity than any traditional extraction technique. This paper describes a three-phase HF-LPME method for ibuprofen using a polypropylene membrane supporting dihexyl ether followed by a chemiluminescence (CL) determination using the CL enhancement on the acidic permanganate-sulfite system in a FIA configuration which is the first time that both techniques have been combined for analytical purposes. The CL intensity (peak area) was proportional to the log of ibuprofen concentration in the donor phase over the range 0.1-20 microg mL(-1). The detection limit was 0.03 microg mL(-1) of ibuprofen in the donor phase. The method was satisfactory reproducible and has been applied to the ibuprofen determination in pharmaceuticals and in real human urine samples.
Payán
María Ramos
MR
Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, Seville-41012, Spain.
López
Miguel Angel Bello
MA
Fernández-Torres
Rut
R
Navarro
Mercedes Villar
MV
Mochón
Manuel Callejón
MC
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000302:isolation & purification* / Q000652:urine; D005591:Chemical Fractionation / Q000379:methods*; D017022:Flow Injection Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000032:analysis* / Q000302:isolation & purification* / Q000652:urine; D007700:Kinetics; D016014:Linear Models; D008163:Luminescent Measurements; D017895:Manganese Compounds / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D017895:Manganese Compounds; D010087:Oxides; D004364:Pharmaceutical Preparations; D013447:Sulfites; C048856:permanganic acid; D007052:Ibuprofen