Development of a method for the determination of gamma-aminobutyric acid in foodstuffs.
A method for the determination of gamma-aminobutyric acid (GABA) in foodstuffs has been developed by combination of its dinitrophenylation and high-performance liquid chromatography (HPLC) using norleucine as an internal standard. GABA was converted to its stable derivative with 1-fluoro-2,4-dinitrobenzene and the derivative was extracted with ether. After evaporation of the extract, the residue was dissolved in 0.1 M NaOH and the solution was subjected to reversed-phase HPLC with an elution system of a linear gradient of methanol in 10 mM Tris-HCl buffer (pH 6.0) and a detection system monitoring the absorbance of the effluent at 400 nm. The present method was shown to be utilized as a satisfactory method for the determination of gamma-aminobutyric acid in foodstuffs.
Ishikawa
Akiko
A
Department of Nutritional Science, Okayama Prefectural University, Soja, Japan.
Oka
Hiroyuki
H
Hiemori
Miki
M
Yamashita
Hiromi
H
Kimoto
Masumi
M
Kawasaki
Hitoshi
H
Tsuji
Hideaki
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004139:Dinitrofluorobenzene / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D009646:Norleucine / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry
D005680:gamma-Aminobutyric Acid; D009646:Norleucine; D004139:Dinitrofluorobenzene