title: Adipocyte differentiation-related protein and OXPAT in rat and human skeletal muscle: involvement in lipid accumulation and type 2 diabetes mellitus.
abstract: METHODS
  A disordered lipid metabolism is implicated in the development of skeletal muscle insulin resistance. Lipid droplet proteins of the PAT [perilipin, adipocyte differentiation-related protein (ADRP), and TIP47] family have been shown to regulate lipid accumulation and intracellular metabolism in other tissues.
  
  
  OBJECTIVE
  This study aimed to explore the role of the PAT proteins OXPAT and ADRP in skeletal muscle lipid metabolism and their putative role in modulating insulin sensitivity.
  
  
  METHODS
  Muscle OXPAT and ADRP protein content was examined during the development of insulin resistance in Zucker diabetic fatty (ZDF) rats and in type 2 diabetes patients and BMI-matched control subjects. Furthermore, we examined the effect of 8 wk of insulin sensitizing by rosiglitazone on muscle OXPAT and ADRP content.
  
  
  RESULTS
  OXPAT and ADRP protein expression is muscle fiber type specific in humans and rats, with highest protein content in fibers containing most intramyocellular lipids (IMCL). Muscle OXPAT and ADRP protein content was 2- to 3-fold higher in ZDF rats during the progression of type 2 diabetes than in lean normoglycemic control rats, which was paralleled by high IMCL levels. Muscle OXPAT and ADRP content, as well as IMCL level, was not different between type 2 diabetes patients and control subjects. ADRP content was negatively associated with insulin-stimulated glucose uptake (r = -0.50; P = 0.017). Interestingly, rosiglitazone treatment decreased muscle OXPAT (-29%) and ADRP (-28%) content in diabetes patients, without affecting IMCL.
  
  
  CONCLUSIONS
  These results indicate involvement of OXPAT and ADRP in muscular lipid accumulation and type 2 diabetes.
authors: NUTRIM School for Nutrition, Toxicology, and Metabolism, Department of Human Movement Sciences, Maastricht University Medical Center+, NL-6200 MD Maastricht, The Netherlands.
authors: Ronnie
authors: R
authors: Minnaard
authors: Patrick
authors: P
authors: Schrauwen
authors: Gert
authors: G
authors: Schaart
authors: Johanna A
authors: JA
authors: Jorgensen
authors: Ellen
authors: E
authors: Lenaers
authors: Marco
authors: M
authors: Mensink
authors: Matthijs K C
authors: MK
authors: Hesselink
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism / Q000502:physiology*; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000138:chemical synthesis / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D000070778:Perilipin-1; D000070780:Perilipin-2; D000070796:Perilipin-3; D000070797:Perilipin-5; D010750:Phosphoproteins / Q000378:metabolism*; D011257:Pregnancy Proteins / Q000378:metabolism*; D051381:Rats; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D033921:Vesicular Transport Proteins
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D007004:Hypoglycemic Agents; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; C000602982:PLIN2 protein, human; C038196:PLIN3 protein, human; C544352:PLIN5 protein, human; C544353:PLIN5 protein, rat; D000070778:Perilipin-1; D000070780:Perilipin-2; D000070796:Perilipin-3; D000070797:Perilipin-5; D010750:Phosphoproteins; C000602984:Plin2 protein, rat; D011257:Pregnancy Proteins; D045162:Thiazolidinediones; D033921:Vesicular Transport Proteins; D000077154:Rosiglitazone