Chemical changes in aging Drosophila melanogaster.
The “Green Theory” of aging proposes that organismal lifespan is limited by the failure to repair molecular damage generated by a broad range of metabolic processes. Two specific predictions arise from this: (1) that these processes will produce a wide variety of stable but dysfunctional compounds that increase in concentration with age, and (2) that organisms maintained under conditions that extend lifespan will display a reduced rate of accumulation of such “molecular rubbish”. To test these predictions, novel analytical techniques were developed to investigate the accumulation of damaged compounds in Drosophila melanogaster. Simple preparative techniques were developed to produce digests of whole D. melanogaster for use in three-dimensional (3D) fluorimetry and 1H NMR spectrometry. Cohorts of Drosophila maintained under normal conditions showed an age-related increase in signals consistent with damage whereas those maintained under conditions of low temperature and dietary restriction did not. 1H NMR revealed distinct age-associated spectral changes that will facilitate the identification of novel compounds that both increase and decrease during aging in this species. These findings are consistent with the predictions of the “Green Theory”.
Iqbal
Aamira
A
School of Pharmacy and Biomolecular Sciences, University of Brighton, Moulsecoomb, Brighton, UK.
Piper
Matthew
M
Faragher
Richard G A
RG
Naughton
Declan P
DP
Partridge
Linda
L
Ostler
Elizabeth L
EL
D000375:Aging / Q000502:physiology*; D000818:Animals; D031204:Caloric Restriction; D004331:Drosophila melanogaster / Q000737:chemistry*; D005260:Female; D005470:Fluorometry; D017127:Glycation End Products, Advanced / Q000032:analysis*; D021621:Imaging, Three-Dimensional; D007814:Larva / Q000737:chemistry / Q000254:growth & development; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*
D017127:Glycation End Products, Advanced