The use of bioluminescence stimulant on the immobilized strain, P. putida mt-2 KG1206, with toluene analog inducers and environmental samples.
This study was conducted to investigate the applicability of the stimulant conditions for the bioluminescence activity of a recombinant strain of Pseudomonas putida, mt-2 KG1206, when immobilized using alginate polymer. The bioluminescence activity of the immobilized strain was generally approximately three to five times lower than the subcultured strain, and the activity was observed to slowly decrease. These facts may have been caused by several factors, such as the low biomass and the time required for diffusion into the entrapped biomass. Although different inducers produced different degrees of stimulation, immobilized bacteria modified with KNO(3) consistently produced more bioluminescence than those treated with sodium lactate, regardless of the inducer chemical tested. Cells treated with KNO(3) exhibited 2.8 times greater bioluminescence than that of the control activity. This condition also stimulated the bioluminescence activities of the immobilized bacteria exposed to contaminated groundwater samples. Based on these results, the immobilized KG1206 presented in this research can be used as a portable assay for the purpose of preliminary on-site monitoring of specific inducer contaminants, with subsequent off-site instrumental analysis, suggesting the potential of this immobilized cell for preliminary application in a field-ready bioassay.
Ko
Kyungsok
K
Geologic Environment Division, Korea Institute of Geoscience & Mineral Resources (KIGAM), Daejon, 305-350, South Korea.
Kong
In Chul
IC
D018914:Cells, Immobilized / Q000187:drug effects / Q000378:metabolism; D049449:Luminescence; D009566:Nitrates / Q000378:metabolism; D017680:Potassium Compounds / Q000378:metabolism; D016958:Pseudomonas putida / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014050:Toluene / Q000031:analogs & derivatives* / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism
D009566:Nitrates; D017680:Potassium Compounds; D014874:Water Pollutants, Chemical; D014050:Toluene; C023844:potassium nitrate