Naturally occurring and synthetic peptides acting on nicotinic acetylcholine receptors.
Nicotinic acetylcholine receptors (nAChRs) are pentameric membrane-bound proteins belonging to the large family of ligand-gated ion channels. nAChRs possess various binding sites which interact with compounds of different chemical nature, including peptides. Historically first peptides found to act on nAChR were synthetic fragments of snake alpha-neurotoxins, competitive receptor antagonists. Later it was shown that fragments of glycoprotein from rabies virus, having homology to alpha-neurotoxins, and polypeptide neurotoxins waglerins from the venom of Wagler's pit viper Trimeresurus (Tropidolaemus) wagleri bind in a similar way, waglerins being efficient blockers of muscle-type nAChRs. Neuropeptide substance P appears to interact with the channel moiety of nAChR. beta-Amyloid, a peptide forming senile plaques in Alzheimer's disease, also can bind to nAChR, although the mode of binding is still unclear. However, the most well-studied peptides interacting with the ligand-binding sites of nAChRs are so-called alpha-conotoxins, peptide neurotoxins from marine snails of Conus genus. First alpha-conotoxins were discovered in the late 1970s, and now it is a rapidly growing family due to isolation of peptides from multiple Conus species, as well as to cloning, and chemical synthesis of new analogues. Because of their unique selectivity towards distinct nAChR subtypes, alpha-conotoxins became valuable tools in nAChR research. Recent X-ray structures of alpha-conotoxin complexes with acetylcholine-binding protein, a model of nAChR ligand-binding domains, revealed the details of the nAChR ligand-binding sites and provided the basis for design of novel ligands.
Kasheverov
Igor E
IE
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Utkin
Yuri N
YN
Tsetlin
Victor I
VI
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D020916:Conotoxins / Q000737:chemistry; D003435:Crotalid Venoms / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D015699:HIV Envelope Protein gp120 / Q000737:chemistry; D008024:Ligands; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry; D018733:Nicotinic Antagonists / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000737:chemistry*; D013373:Substance P / Q000737:chemistry
D016229:Amyloid beta-Peptides; D020916:Conotoxins; D003435:Crotalid Venoms; D006023:Glycoproteins; D015699:HIV Envelope Protein gp120; D008024:Ligands; D009498:Neurotoxins; D018733:Nicotinic Antagonists; D010455:Peptides; D011978:Receptors, Nicotinic; C077895:waglerin; D013373:Substance P