Immune responses induced by a BacMam virus expressing the E2 protein of classical swine fever virus in mice.
Non-replicating baculovirus-mediated gene transfer into mammalian cells has been developed as a vaccine strategy against a number of diseases in several animal models. In the present study, the BacMam vector, a baculovirus pseudotyped with the glycoprotein from vesicular stomatitis virus, was used as a recombinant vector to express classical swine fever virus (CSFV) E2 protein under the control of the immediate early 1 (ie1) promoter from shrimp white spot syndrome virus. The E2 gene was efficiently expressed in both insect and mammalian cells. Intramuscular injection of mice with the recombinant baculovirus resulted in the production of high-titers of CSFV-specific neutralizing antibodies. Specific lymphoproliferative responses to CSFV stimulation were detected in the splenocytes of the immunized mice as demonstrated by CFSE staining assay and WST-8 assay. This study demonstrates that the BacMam virus vector can efficiently express the E2 protein and effectively induce immune responses against CSFV. This is a first step in the demonstration that the pseudotyped baculovirus-delivered CSFV E2 gene can be a potential non-replicating vaccine against CSFV infections.
Li
Miao
M
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001 Harbin, Heilongjiang, China.
Wang
Yu-Fei
YF
Wang
Yu
Y
Gao
Hui
H
Li
Na
N
Sun
Yuan
Y
Liang
Bing-Bing
BB
Qiu
Hua-Ji
HJ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016367:Baculoviridae / Q000235:genetics* / Q000472:pathogenicity; D049109:Cell Proliferation; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000235:genetics* / Q000276:immunology; D004175:Diptera / Q000235:genetics / Q000821:virology; D005260:Female; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D056724:Immunity, Humoral; D007114:Immunization; D007273:Injections, Intramuscular; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology* / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D033561:Penaeidae / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D013154:Spleen / Q000276:immunology / Q000473:pathology / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines; D014774:Virulence; D046848:White spot syndrome virus 1 / Q000235:genetics
D000914:Antibodies, Viral; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus