Using the larvae nematode Caenorhabditis elegans to evaluate neurobehavioral toxicity to metallic salts.
In this study, we investigated the locomotion behavior changes at different developmental stages in Caenorhabditis elegans exposed to metals for 4h. No obvious differences could be observed in young adults exposed to examined metals, and only exposure to 100 microM of examined metals could significantly decrease the locomotion behaviors of L4 larvae. In contrast, exposure to 50 and 100 microM of examined metals induced noticeable repression of locomotion behaviors at L1-L3 larval stages, and a significant decrease of locomotion behaviors could be observed in L1 larvae exposed to Pb and Hg, and in L2 larvae exposed to Hg at the concentration of 2.5 microM. Moreover, the L1-, L2-, and L3-larvae exposed to metals for 4h exhibited similar neurobehavioral toxicity manner to L4-larvae exposed to metals for 24h. Therefore, younger larvae showed more severe deficits in neurobehavioral phenotypes than L4 larvae and young adults in metal-exposed nematodes.
Xing
Xiaojuan
X
Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Department of Genetics and Developmental Biology, Southeast University Medical School, Nanjing 210009, China.
Guo
Yuling
Y
Wang
Dayong
D
D000375:Aging / Q000187:drug effects*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D004785:Environmental Pollutants / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D019216:Metals, Heavy / Q000633:toxicity*; D009043:Motor Activity / Q000187:drug effects*; D018675:Toxicity Tests
D004785:Environmental Pollutants; D019216:Metals, Heavy