Detectable effect size and bioassay power of mummichog (Fundulus heteroclitus) and fathead minnow (pimephales promelas) adult reproductive tests.
Although multiple reproductive tests have been developed in small-bodied fish to determine the effects of endocrine-disrupting substances, few direct comparisons have been made among the available tests. Side-by-side reproductive tests with mummichog (Fundulus heteroclitus) and fathead minnow (FHM; Pimephales promelas) were conducted with 0, 3, 10, and 30% effluent from a bleached kraft pulp mill in Saint John, New Brunswick, Canada. Egg production was significantly increased in mummichog exposed to 3% combined mill effluent, but no difference was observed in FHM. No differences were found in whole-body testosterone or estradiol levels in mummichog, and whole-body 11-ketotestosterone levels in males were increased in 3% exposed fish compared to those in 10% effluent. Male FHM exposed to 30% effluent had increased whole-body testosterone levels, and female FHM in 30% effluent had decreased testosterone. No differences in estradiol or 11-ketotestosterone were observed in FHM. Relatively limited response occurred in other endpoints. A comparison of the results of the present study to other published studies suggests that current reproductive bioassays are only sensitive for detecting magnitudes of change of greater than 50% and that differences exist in the sensitivities of fish. Future research should address methods of reducing variability within test populations and focus on understanding the comparative responses among species commonly used for endocrine-disrupting substance testing.
Melvin
Steven D
SD
Department of Biology and Canadian Rivers Institute, University of New Brunswick, Saint John, New Brunswick E2L 4L5.
Munkittrick
Kelly R
KR
Bosker
Thijs
T
MacLatchy
Deborah L
DL
D000818:Animals; D001681:Biological Assay; D003530:Cyprinidae / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000032:analysis; D005260:Female; D023781:Fundulidae / Q000378:metabolism*; D006066:Gonads / Q000187:drug effects; D008297:Male; D009509:New Brunswick; D012098:Reproduction / Q000187:drug effects*; D013739:Testosterone / Q000031:analogs & derivatives / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D052244:Endocrine Disruptors; D014874:Water Pollutants, Chemical; D013739:Testosterone; D004958:Estradiol; C003600:11-ketotestosterone