Using polarization analysis to separate the coherent and incoherent scattering from protein samples.
Polarization analysis was used to separate experimentally the coherent and spin-incoherent nuclear static scattering functions, from a representative set of samples of interest for protein studies. This method had so far limited application in the study of amorphous materials, despite the relevance of the information that it provides. It allows, for instance, the experimental determination of the structure factor of materials containing a significant amount of hydrogen atoms, avoiding the contamination of measurements by a non-negligible incoherent background. Knowledge of the relative importance of the coherent and incoherent terms at different Q-values is also a pre-requisite for the interpretation of quasielastic neutron scattering experiments, performed at instruments in which the total dynamic scattering function is measured, such as conventional time-of-flight and backscattering spectrometers. Combining data from different instruments, it was possible to cover a wide Q-range, from the small-angle region (0.006<Q<0.04 A(-1)) to the wide-angle region (up to approximately 2.35 A(-1)). Quantitative information was obtained on the fraction of coherent to spin-incoherent scattering from different protein samples: deuterated and protonated protein powders at different hydration levels and solutions of protonated proteins in D(2)O at different concentrations. The results obtained are discussed in the context of the validity of the assumptions generally made when interpreting quasielastic neutron scattering experiments performed without polarization analysis.
Gaspar
Ana M
AM
Physik Department E13, Technische Universität München, Germany. ana.gaspar@frm2.tum.de
Busch
Sebastian
S
Appavou
Marie-Sousai
MS
Haeussler
Wolfgang
W
Georgii
Robert
R
Su
Yixi
Y
Doster
Wolfgang
W
D017666:Deuterium Oxide / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D008956:Models, Chemical; D009211:Myoglobin / Q000737:chemistry*; D033363:Neutron Diffraction / Q000379:methods; D010798:Phycocyanin / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012996:Solutions; D014867:Water / Q000737:chemistry*
D006454:Hemoglobins; D009211:Myoglobin; D011506:Proteins; D012996:Solutions; D014867:Water; D010798:Phycocyanin; D017666:Deuterium Oxide