ICAM-5 modulates cytokine/chemokine production in the CNS during the course of herpes simplex virus type 1 infection.
Chemokines are important in HSE development in the CNS but underlying regulatory events are unknown. Two-hybrid binding assays identified that intercellular adhesion molecule 5 (ICAM-5), an immune modulator in the CNS, interacted with neurovirulence factor, UOL, of HSV-1. Viral load and interleukin levels were similar in UOL deletion virus (DeltaUOL), and wild type virus infected mouse brains. However, higher numbers of lymphocytes, but unaltered soluble ICAM-5 and chemokine levels were detected in DeltaUOL infected mouse brains. In contrast, lower lymphocyte numbers, reduced soluble ICAM-5, and higher chemokine levels were detected in wild type virus infected brains. Our results suggest that ICAM-5 plays a critical role in modulating chemokine production in the CNS.
Tse
Margaret C L
MC
Department of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, USA.
Lane
Crystal
C
Mott
Kevin
K
Onlamoon
Nattawat
N
Hsiao
Hui-Mien
HM
Perng
Guey Chuen
GC
D000818:Animals; D001921:Brain / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000276:immunology; D020803:Encephalitis, Herpes Simplex / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000378:metabolism; D007155:Immunologic Factors / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D015854:Up-Regulation / Q000276:immunology; D019562:Viral Load; D037521:Virulence Factors / Q000378:metabolism
D018925:Chemokines; D016207:Cytokines; C504585:Icam5 protein, mouse; D007155:Immunologic Factors; D007378:Interleukins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D037521:Virulence Factors