Pharmacokinetics of pefloxacin mesylate in human urine using capillary electrophoresis electrochemiluminescence detection.
A novel and simple method to determine pharmacokinetics of pefloxacin mesylate (PM) in urine of seven healthy adults was developed. The proposed methodology was based on the electrochemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium (II) at a platinum electrode. The ECL intensity was found greatly enhanced in the presence of PM, which could directly participate in the light-emitting reaction as the reductant (in former/unchanged form). Under optimised conditions, the calibration curve was linear from 0.02 to 12 mg/L with a detection limit of 0.004 mg/L (sigma=3). The RSD of the peak height was less than 2.4% (n=6). The recoveries in human urine were 96.2% to 98.3%. The highest excretion rate in urine was observed during the period 1.5-2 h after oral administration. The urinary excretion ratio of PM was 13.6% within 48 h.
Deng
Biyang
B
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. dengby16@163.com
Li
Linqiu
L
Shi
Aihong
A
Kang
Yanhui
Y
D000328:Adult; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D015366:Pefloxacin / Q000493:pharmacokinetics* / Q000652:urine*; D012680:Sensitivity and Specificity; D055815:Young Adult
D015366:Pefloxacin