Dry-coated microprojection array patches for targeted delivery of immunotherapeutics to the skin.
Dry-coated microprojections (MPs) deliver vaccine to abundant immunogenic cells within the skin to induce immune responses. Success in this targeted vaccine delivery relies on overcoming the challenges of dry-coating the vaccine onto the very small (<or=90 microm length) and densely packed (approximately 20,000 cm(-2)) MPs. In this paper, we show that a gas-jet drying coating method achieves the desired uniform coating. The coating approach is robustly demonstrated on compounds representative of a range of immunotherapeutics (e.g. DNA, proteins), with each uniformly coated on thousands of MPs. Furthermore, the dry-coating remains intact during skin insertion, and then releases within the wet skin cellular environment within 3 min. Finally, we applied ovalbumin protein coated MP patches to immunise mice, achieving comparable antibody levels (p=0.08) with needle and syringe intramuscular injection. In summary, this paper presents a simple, practical and versatile method to achieve uniform coating on very small and densely packed MPs for a needle-free and targeted vaccine delivery technology.
Chen
Xianfeng
X
Delivery of Drugs and Genes Group (D(2)G(2)), Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
Prow
Tarl W
TW
Crichton
Michael L
ML
Jenkins
Derek W K
DW
Roberts
Michael S
MS
Frazer
Ian H
IH
Fernando
Germain J P
GJ
Kendall
Mark A F
MA
D000279:Administration, Cutaneous; D000818:Animals; D000906:Antibodies / Q000097:blood*; D002626:Chemistry, Pharmaceutical; D004304:Dosage Forms; D004339:Drug Compounding; D016503:Drug Delivery Systems; D005260:Female; D007167:Immunotherapy / Q000379:methods*; D007273:Injections, Intramuscular; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D036103:Nanotechnology; D010047:Ovalbumin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D012869:Skin Absorption; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics
D000906:Antibodies; D004304:Dosage Forms; D014612:Vaccines; D010047:Ovalbumin