Elucidating the mechanism of lipid membrane-induced IAPP fibrillogenesis and its inhibition by the red wine compound resveratrol: a synchrotron X-ray reflectivity study.
The islet amyloid polypeptide (IAPP) or amylin is a pancreatic hormone and crucially involved in the pathogenesis of type-II diabetes mellitus (T2DM). Aggregation and amyloid formation of IAPP is considered as the primary culprit for pancreatic beta-cell loss in T2DM patients. In this study, first X-ray reflectivity (XRR) measurements on IAPP at lipid interfaces have been carried out, providing a molecular level characterization of the first steps of the lipid-induced fibrillation process of IAPP, which is initiated by lipid-induced nucleation, oligomerization, followed by detachment of larger IAPP aggregate structures from the lipid membrane, and terminated by the formation of mature fibrils in the bulk solution. The adsorption process of IAPP at lipid interfaces in the absence and presence of negatively charged lipid has also been studied by complementary ATR-FTIR spectroscopic measurements. The morphological properties were followed by atomic force microscopy (AFM). Moreover, we show that the polyphenolic red wine compound resveratrol is able to inhibit IAPP aggregation also in the presence of aggregation-fostering negatively charged lipid interfaces, revealing its potential as a drug candidate for T2DM.
Evers
Florian
F
Faculty of Chemistry, TU Dortmund, 44221 Dortmund, Germany.
Jeworrek
Christoph
C
Tiemeyer
Sebastian
S
Weise
Katrin
K
Sellin
Daniel
D
Paulus
Michael
M
Struth
Bernd
B
Tolan
Metin
M
Winter
Roland
R
D000682:Amyloid / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D058228:Islet Amyloid Polypeptide; D008563:Membrane Lipids; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D000077185:Resveratrol; D017550:Spectroscopy, Fourier Transform Infrared; D013267:Stilbenes / Q000378:metabolism*; D014965:X-Rays
D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D008563:Membrane Lipids; D013267:Stilbenes; D000077185:Resveratrol