title: Binge drinking upregulates accumbens mGluR5-Homer2-PI3K signaling: functional implications for alcoholism.
abstract: The glutamate receptor-associated protein Homer2 regulates alcohol-induced neuroplasticity within the nucleus accumbens (NAC), but the precise intracellular signaling cascades involved are not known. This study examined the role for NAC metabotropic glutamate receptor (mGluR)-Homer2-phosphatidylinositol 3-kinase (PI3K) signaling in regulating excessive alcohol consumption within the context of the scheduled high alcohol consumption (SHAC) model of binge alcohol drinking. Repeated bouts of binge drinking ( approximately 1.5 g/kg per 30 min) elevated NAC Homer2a/b expression and increased PI3K activity in this region. Virus-mediated knockdown of NAC Homer2b expression attenuated alcohol intake, as did an intra-NAC infusion of the mGluR5 antagonist MPEP [2-methyl-6-(phenylethynyl)pyridine hydrochloride] (0.1-1 microg/side) and the PI3K antagonist wortmannin (50 ng/side), supporting necessary roles for mGluR5/Homer2/PI3K in binge alcohol drinking. Moreover, when compared with wild-type littermates, transgenic mice with an F1128R point mutation in mGluR5 that markedly reduces Homer binding exhibited a 50% reduction in binge alcohol drinking, which was related to reduced NAC basal PI3K activity. Consistent with the hypothesis that mGluR5-Homer-PI3K signaling may be a mechanism governing excessive alcohol intake, the "anti-binge" effects of MPEP and wortmannin were not additive, nor were they observed in the mGluR5(F1128R) transgenic mice. Finally, mice genetically selected for a high versus low SHAC phenotype differed in NAC mGluR, Homer2, and PI3K activity, consistent with the hypothesis that augmented NAC mGluR5-Homer2-PI3K signaling predisposes a high binge alcohol-drinking phenotype. Together, these data point to an important role for NAC mGluR5-Homer2-PI3K signaling in regulating binge-like alcohol consumption that has relevance for our understanding of the neurobiology of alcoholism and its pharmacotherapy.
authors: Department of Psychology and the Neuroscience Research Institute, University of California at Santa Barbara, Santa Barbara, California 93106-9660, USA.
authors: Debra K
authors: DK
authors: Cozzoli
authors: Scott P
authors: SP
authors: Goulding
authors: Ping Wu
authors: PW
authors: Zhang
authors: Bo
authors: B
authors: Xiao
authors: Jia-Hua
authors: JH
authors: Hu
authors: Alexis W
authors: AW
authors: Ary
authors: Ilona
authors: I
authors: Obara
authors: Alison
authors: A
authors: Rahn
authors: Hoda
authors: H
authors: Abou-Ziab
authors: Burgundy
authors: B
authors: Tyrrel
authors: Christina
authors: C
authors: Marini
authors: Naomi
authors: N
authors: Yoneyama
authors: Pamela
authors: P
authors: Metten
authors: Christopher
authors: C
authors: Snelling
authors: Marlin H
authors: MH
authors: Dehoff
authors: John C
authors: JC
authors: Crabbe
authors: Deborah A
authors: DA
authors: Finn
authors: Matthias
authors: M
authors: Klugmann
authors: Paul F
authors: PF
authors: Worley
authors: Karen K
authors: KK
authors: Szumlinski
mesh_terms: D000437:Alcoholism / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000431:Ethanol / Q000633:toxicity*; D000072233:Homer Scaffolding Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009714:Nucleus Accumbens / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010641:Phenotype; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; C578936:Grm5 protein, mouse; D000072233:Homer Scaffolding Proteins; C000606625:Homer2 protein, mouse; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; D000431:Ethanol; D019869:Phosphatidylinositol 3-Kinases