title: Expression pattern of prmt5 in adult fish and embryos of medaka, Oryzias latipes.
abstract: DNA methylation is an important biochemical epigenetic determinant of gene expression in cells and therefore actively involved in gene regulation, chromosomal conformation, and protein activity. Protein arginine methyltransferases (PRMTs) play a major role in the methylation of proteins that have an arginine residue, catalyzing both the asymmetric dimethylation of arginine (aDMA) and symmetric dimethylation of arginine (sDMA). PRMT5, a type II PRMT which catalyzes sDMA, has been shown to have a pivotal role in pole plasm assembly and germ cell development in Drosophila and also to be an associate factor of Blimp1 for germ cell development in mouse. Here, we report a homolog of prmt5 identified in medaka, Oryzias latipes, which was detected in the brain, gill, muscle, heart, liver, spleen, intestine, testis and ovary of adult fish by reverse transcriptase-PCR. The expression of prmt5 in the gonads is restricted to oocytes of the ovary, spermatogonia, and spermatocytes of testis. The prmt5 transcripts were detected as early as the one-cell stage and in all the tissues of embryos during embryogenesis. In summary, prmt5 is a maternal determinant factor of embryogenesis of medaka, possibly playing an important role in oogenesis and spermatogenesis in adult medaka.
authors: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Huazhong Normal University, Wuhan, 430079, China.
authors: W
authors: W
authors: Chen
authors: M
authors: M
authors: Cao
authors: Y
authors: Y
authors: Yang
authors: Y
authors: Y
authors: Nagahama
authors: H
authors: H
authors: Zhao
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009990:Oryzias / Q000196:embryology* / Q000378:metabolism*; D010802:Phylogeny; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D011484:Protein-Arginine N-Methyltransferases