Polymorphisms in the promoter region of the interleukin-10 (IL-10) gene in women with cervical insufficiency.
OBJECTIVE
Our objective was to determine whether polymorphisms in the promoter region of the interleukin-10 gene are more common in women with cervical insufficiency compared with controls.


METHODS
We conducted a case-control study. Histories and blood were obtained from 121 cases and 157 controls. DNA was extracted and purified by using Puregene kits. Samples were analyzed for the interleukin-10 -1082 G/A polymorphism and interleukin-10.G microsatellite in the interleukin-10 gene promoter region.


RESULTS
The -1082 G/A polymorphism in the promoter region of the interleukin-10 gene occurred with similar frequency in cases and controls. The interleukin-10.G microsatellite contained 10 alleles (G6-G16). The G13 allele was present more frequently in cases (24.1%) compared with controls (14.6%) (P = .05).


CONCLUSIONS
Interleukin-10 is known to down-regulate inflammation. The G13 allele in the interleukin-10.G microsatellite occurred more frequently in women with cervical insufficiency compared with controls, suggesting that alterations in inflammatory processes may play a role in cervical insufficiency.
Warren
Jennifer E
JE
Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA. Jennifer.warren@hsc.utah.edu
Nelson
Lesa M
LM
Stoddard
Gregory J
GJ
Esplin
M Sean
MS
Varner
Michael W
MW
Silver
Robert M
RM
D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D011110:Polymorphism, Genetic; D047928:Premature Birth / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D002581:Uterine Cervical Incompetence / Q000235:genetics*
D016753:Interleukin-10