title: [Polymorphism and forensic applications of miniSTR loci D10S1248, D2S441, D1S1677, D9S1122, and D10S1435 in Northwestern Han population].
abstract: To investigate the polymorphism, genetic stability and the value for analysis degraded samples of D10S1248/ D2S441/D1S1677/D9S1122/D10S1435 five loci in northwestern Han population. The lengths of fragments were analyzed by fluorescence PCR and ABI310 Genetic Analyzer. Samples from 154 unrelated northwestern Han individuals, 10 genealogies and 10 highly degraded specimens were genotyped. Among the 154 unrelated northwestern Han individuals, we discovered 8, 7, 7, 6, and 7 alleles in the five miniSTR loci, respectively. The frequency distributions in the loci showed no deviations from Hardy-Weinberg equilibrium expectations. The H is at 0.662-0.792, PD is at 0.869-0.915, PE is at 0.382-0.585, and PIC is at 0.650-0.750.The research of genetic stability and the value for analysis degraded sample indicated that the five miniSTR loci had high genetic stability and could analysis the degraded samples effectively. Therefore, these 5 miniSTR loci can be used as genetic markers of Northwestern Han populations in forensic practice involving individual identification and paternity testing from degraded DNA samples.
authors: Department of Forensic Biology, Hebei Medical University, Shijiazhuang 050017, China. snowhome2004@126.com
authors: Xue
authors: X
authors: Bai
authors: Bin
authors: B
authors: Cong
authors: Shu-Jin
authors: SJ
authors: Li
authors: Xia
authors: X
authors: Guo
authors: Xia
authors: X
authors: Li
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*