Kinetic resolution of rac-1-phenylethanol with immobilized lipases: a critical comparison of microwave and conventional heating protocols.
The lipase-catalyzed kinetic resolution of rac-1-phenylethanol with vinyl acetate as acyl donor and cyclohexane as solvent has been investigated applying both microwave dielectric heating and conventional thermal heating in order to probe the existence of nonthermal microwave effects. All transformations were conducted at 40 degrees C in a dedicated reactor setup that allowed accurate internal reaction temperature measurements with use of fiber-optic probes. Quartz reaction vessels that allow higher levels of microwave power to be administered to the reaction mixture were used for all experiments. For all five studied immobilized lipases, the observed reactivities and enantioselectivities in microwave and oil bath experiments were identical and thus not related to the presence of the microwave field. The effect of magnetic stirring proved critical as too rapid stirring in some instances destroyed the enzyme support structure and led to altered reactivities and selectivities.
de Souza
Rodrigo Octavio M A
RO
Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Bloco A 641, RJ 21941-909, Brazil.
Antunes
Octavio A C
OA
Kroutil
Wolfgang
W
Kappe
C Oliver
CO
D055162:Biocatalysis; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008872:Microwaves; D010626:Phenylethyl Alcohol / Q000737:chemistry* / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology; D013237:Stereoisomerism
D004800:Enzymes, Immobilized; D008049:Lipase; D010626:Phenylethyl Alcohol