title: Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics.
abstract: The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL).
authors: Department of Electrical Measurements, Lund University, Box 118, 221 00 Lund, Sweden. andreas.lenshof@elmat.lth.se
authors: Andreas
authors: A
authors: Lenshof
authors: Asilah
authors: A
authors: Ahmad-Tajudin
authors: Kerstin
authors: K
authors: Järås
authors: Ann-Margret
authors: AM
authors: Swärd-Nilsson
authors: Lena
authors: L
authors: Aberg
authors: György
authors: G
authors: Marko-Varga
authors: Johan
authors: J
authors: Malm
authors: Hans
authors: H
authors: Lilja
authors: Thomas
authors: T
authors: Laurell
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D007118:Immunoassay; D008297:Male; D046228:Microarray Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010956:Plasmapheresis / Q000295:instrumentation* / Q000379:methods; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis; D040081:Protein Array Analysis; D012825:Silicon / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D017430:Prostate-Specific Antigen; D012825:Silicon