Early life DNA vaccination with the H gene of Canine distemper virus induces robust protection against distemper.
Young mink kits (n=8) were vaccinated with DNA plasmids encoding the viral haemagglutinin protein (H) of a vaccine strain of Canine distemper virus (CDV). Virus neutralising (VN) antibodies were induced after 2 immunisations and after the third immunisation all kits had high VN antibody titres. The VN antibody titres remained high for more than 4 months and the mink were protected against viraemia, lymphopenia, clinical disease and changes in the percentage of IFN-gamma producing peripheral blood leucocytes after challenge inoculation with a recent wild type strain of CDV. Essentially, these results demonstrate that early life DNA vaccination with the H gene of a CDV vaccine strain induced robust protective immunity against a recent wild type CDV.
Jensen
Trine Hammer
TH
Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark.
Nielsen
Line
L
Aasted
Bent
B
Blixenkrone-Møller
Merete
M
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002522:Chlorocebus aethiops; D004216:Distemper / Q000276:immunology / Q000517:prevention & control*; D004217:Distemper Virus, Canine / Q000276:immunology*; D006389:Hemagglutinins, Viral / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D008907:Mink / Q000276:immunology* / Q000821:virology; D009500:Neutralization Tests; D010957:Plasmids; D019444:Vaccines, DNA / Q000276:immunology; D014709:Vero Cells; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines; D007371:Interferon-gamma