Integrated microfluidic electrochemical DNA sensor.
Effective systems for rapid, sequence-specific nucleic acid detection at the point of care would be valuable for a wide variety of applications, including clinical diagnostics, food safety, forensics, and environmental monitoring. Electrochemical detection offers many advantages as a basis for such platforms, including portability and ready integration with electronics. Toward this end, we report the Integrated Microfluidic Electrochemical DNA (IMED) sensor, which combines three key biochemical functionalities--symmetric PCR, enzymatic single-stranded DNA generation, and sequence-specific electrochemical detection--in a disposable, monolithic chip. Using this platform, we demonstrate detection of genomic DNA from Salmonella enterica serovar Typhimurium LT2 with a limit of detection of <10 aM, which is approximately 2 orders of magnitude lower than that from previously reported electrochemical chip-based methods.
Ferguson
Brian S
BS
Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, USA.
Buchsbaum
Steven F
SF
Swensen
James S
JS
Hsieh
Kuangwen
K
Lou
Xinhui
X
Soh
H Tom
HT
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques; D020411:Oligonucleotide Array Sequence Analysis; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism
D004269:DNA, Bacterial