Clinical determinants of ckit-positive cardiac cell yield in coronary disease.
Recent animal studies and clinical trials have reported the scope of heart-resident ckit-positive stem cells in regenerating infarcted myocardium. The determinants of successful isolation of such cells are unknown. The objective of this study was to determine the influence of risk factors for coronary artery disease and disease severity on the successful isolation of ckit-positive cells from right atrial tissue of patients with coronary artery disease. The findings suggest that coronary artery disease and cardiac remodeling in chronic ischemia may not affect the yield of ckit-positive cells from atrial tissue, but a significant negative correlation between the age of the patient and the number of migrated ckit-positive cells was observed. This suggests that in older patients, stem cell isolation from cardiac biopsies may not succeed, and such cells may not be available for cell therapy.
Aghila Rani
Koippallil G
KG
Division of Cellular & Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum-695011, India.
Jayakumar
Karunakaran
K
Sarma
P Sankara
PS
Kartha
Chandrasekharan C
CC
D000328:Adult; D000367:Age Factors; D000368:Aged; D001706:Biopsy; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003324:Coronary Artery Disease / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D006325:Heart Atria / Q000378:metabolism / Q000473:pathology; D006801:Humans; D016014:Linear Models; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D012307:Risk Factors; D012720:Severity of Illness Index; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology
D019009:Proto-Oncogene Proteins c-kit