[Expression and regulation of megalin in gallbladder mucosa associated with cholesterol gallstone disease].
OBJECTIVE
To explore the relationship between expression and regulation of Megalin in gallbladder mucosa and cholesterol gallstone disease.


METHODS
Gallbladder mucosa, gallbladder wall, bile, gallstone were collected from 29 patients with cholesterol gallstone disease (GS) and 12 patients with gallstone free (GSF). Lipids of bile and stone were measured by kits. Expression of Megalin and Cubilin was analyzed by Real-time PCR. GBC-SD cell line were treated with T0901317, 9-cis retinoic acid, chenodeoxycholic acid (CDCA), the agonists of LXR, RXR, FXR, respectively. Gene expressions were detected.


RESULTS
Biliary cholesterol % molar and CSI increased significantly in GS group [(7.98 +/- 0.44) mol% vs (4.87 +/- 0.39) mol%, P < 0.01]. Megalin expression in GS group was significantly higher than that in GSF group (P < 0.05) and cubilin expression was similar between two groups. In vitro experiments showed that CDCA markedly increased expression of megalin.


CONCLUSIONS
This study shows that the increased expression Megalin may help to increase cholesterol uptake in gallbladder and play a compensative role in GS. FXR may participate in the transcription regulating of Megalin.
Wang
Yong
Y
Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Liu
Ying-Bin
YB
Jiang
Zhao-Yan
ZY
Cai
Qu
Q
Jiang
Zhi-Hong
ZH
Han
Tian-Quan
TQ
Zhang
Sheng-Dao
SD
D002635:Chenodeoxycholic Acid / Q000378:metabolism; D002769:Cholelithiasis / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005704:Gallbladder / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism*
D026561:Low Density Lipoprotein Receptor-Related Protein-2; D002635:Chenodeoxycholic Acid