title: A combined cell column superfusion technique to study hypothalamus-pituitary-adrenal interactions in vitro.
abstract: Rat anterior pituitaries and rat adrenals were dispersed into cell suspensions. In a cell column superfusion system the pituitary cells were used to study ACTH release and the adrenal cells served as a means to detect that hormone. Linear log-dose-response relationships were exhibited by the adrenal cells for ACTH and by the pituitary cell-adrenal cell system for corticotropin-releasing factor (CRF). A combined system, constructed of a superfused hypothalamus, leading into a column of pituitary cells which was connected to a column of adrenal cells, was used to study the characteristics of CRF release from the hypothalamic block. Basal and stimulated CRF release were both Ca(2+)-dependent.
authors: Free University, Medical Faculty, Department of Pharmacology, Amsterdam, The Netherlands.
authors: G H
authors: GH
authors: Mulder
authors: I
authors: I
authors: Vermes
authors: P G
authors: PG
authors: Smelik