Magnetic-fluorescent colloidal nanobeads: preparation and exploitation in cell separation experiments.
Nanostructures displaying fluorescence and magnetic properties at the same time are potentially useful for achieving simultaneous bio-separation and bio-sensing (e.g., magnetic separation coupled with multiplexing optical detection of different tumour cell populations). Spherical nanobeads that display both fluorescent and magnetic features are reported; they are fabricated by grafting fluorescent oligothiophene molecules to an amphiphilic polymer that is then used to enwrap iron oxide nanoparticles, which acts as the magnetic domain. By tuning experimental conditions, control over the number of magnetic nanoparticles per bead and over the bead diameter (30-400 nm) was achieved. A cell separation efficiency of the level required for cell sorting applications is also reported.
Di Corato
Riccardo
R
National Nanotechnology Laboratory of CNR-INFM, Lecce, Italy.
Piacenza
Philomena
P
Musarò
Mariarosaria
M
Buonsanti
Raffaella
R
Cozzoli
Pantaleo Davide
PD
Zambianchi
Massimo
M
Barbarella
Giovanna
G
Cingolani
Roberto
R
Manna
Liberato
L
Pellegrino
Teresa
T
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D003102:Colloids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008280:Magnetics; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
D003102:Colloids; D005456:Fluorescent Dyes