Development of HPLC fingerprints for Mallotus species extracts and evaluation of the peaks responsible for their antioxidant activity.
Some Mallotus species are used in traditional medicine in Vietnam. To use certain species in Western medicines or as food supplements, they should be identified and quality control should be more strict, for instance, to avoid the erroneous switching of species. In species with interesting activities, the compounds responsible for them should be identified. For these identifications, HPLC fingerprint methodology can be used. In this paper, HPLC fingerprints of different lengths were developed for a number of Mallotus species. Secondly, a multivariate regression model was constructed to model the antioxidant activity of the Mallotus samples from the HPLC fingerprints with the aim to indicate peaks possibly responsible for this activity. For this purpose, after data pretreatment, the calibration technique partial least squares (PLS) was applied.
Nguyen Hoai
N
N
Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium.
Dejaegher
B
B
Tistaert
C
C
Nguyen Thi Hong
V
V
Rivière
C
C
Chataigné
G
G
Phan Van
K
K
Chau Van
M
M
Quetin-Leclercq
J
J
Vander Heyden
Y
Y
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000737:chemistry; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D019587:Dietary Supplements; D005609:Free Radicals; D007202:Indicators and Reagents / Q000032:analysis / Q000302:isolation & purification; D016018:Least-Squares Analysis; D036461:Mallotus Plant / Q000378:metabolism*; D015999:Multivariate Analysis; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D025341:Principal Component Analysis; D013678:Technology, Pharmaceutical / Q000379:methods
D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D007202:Indicators and Reagents; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl