Micropreparation of single secretory glands from the carnivorous plant Nepenthes.
A rapid mechanical micropreparation technique has been developed to isolate multicellular glands, here from Nepenthes pitchers, based on a microdissection platform. The method is an alternative to laser capture dissection because fresh plant tissue can be used directly without previous fixation. Subsequent experiments, such as polymerase chain reaction (PCR)-based detection of an individual gene encoding a thaumatin-like protein and RNA extraction for gene expression analysis, have been successfully added to prove the quality of the prepared biological material. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of multicellular glands or other tissues.
Rottloff
Sandy
S
Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, D-07745 Jena, Germany.
Müller
Ute
U
Kilper
Roland
R
Mithöfer
Axel
A
D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D019684:Magnoliopsida / Q000033:anatomy & histology* / Q000235:genetics; D042282:Microdissection / Q000379:methods*; D008853:Microscopy; D016133:Polymerase Chain Reaction; D013997:Time Factors