title: [Expression of the voltage-dependent anion channel gene in human ejaculated spermatozoa].
abstract: OBJECTIVE
  To identify the genes involved in sperm motility.
  
  
  METHODS
  We hybridized asthenospermia and normal motile sperm cDNA samples with the human whole genome Affymetrix chip to screen differentially expressed genes. Then we detected the mRNA expressions of the voltage-dependent anion channel genes (VDACs) in human organs and spermatozoa by RT-PCR and compared their expressions in the poor and normal motility spermatozoa.
  
  
  RESULTS
  Differentially expressed genes VDACs were identified by analysis of the hybridization signals, including the 3 subtypes VDAC1, VDAC2 and VDAC3. The expression of VDAC2 mRNA was significantly decreased in the poor motility sperm (0.568 +/- 0.036), as compared with the healthy men (0.803 +/- 0.043, P < 0.01).
  
  
  CONCLUSIONS
  The decreased expression of VDAC2 in the ejaculated spermatozoa is possibly associated with the reduction of sperm motility.
authors: Key Laboratory of Male Reproductive and Genetic Medicine, Peking University Shenzhen Hospital, Shenzhen, Guang-dong 518036, China. xuxiang_hard@163.com
authors: Xiang
authors: X
authors: Xu
authors: Yong
authors: Y
authors: Wang
authors: Zhou
authors: Z
authors: Yu
authors: Jing
authors: J
authors: Chen
authors: Meng
authors: M
authors: Guo
authors: Yao-ting
authors: YT
authors: Gui
authors: Zhi-ming
authors: ZM
authors: Cai
mesh_terms: D055028:Comparative Genomic Hybridization; D015870:Gene Expression; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D050996:Voltage-Dependent Anion Channel 2 / Q000235:genetics*
chemical_list: D050996:Voltage-Dependent Anion Channel 2