Cycloartenyl ferulate, a component of rice bran oil-derived gamma-oryzanol, attenuates mast cell degranulation.
IgE-targeting therapy could provide significant progress in the treatment of allergic inflammation. In this study, we examined the effect of cycloartenyl ferulate (cycloartenol ferulic acid ester; CAF), a natural product from rice bran oil-derived gamma-oryzanol, on allergic reaction. When CAF and gamma-oryzanol were injected intradermally with anti-DNP IgE into the dorsal skin of rats, the passive cutaneous anaphylaxis reaction induced by DNP-HSA was attenuated. CAF and gamma-oryzanol also inhibited the degranulation of DNP-IgE sensitized RBL-2H3 mast cells stimulated with anti-DNP-HSA. IgE conjugated with CAF could not be detected by anti-IgE antibody in the ELISA analysis. Although incubation of IgE with CAF did not decrease the amount of IgE, it was possible to precipitate IgE by centrifugation. These results demonstrate that CAF captures IgE, prevents it from binding to FcepsilonRI, and attenuates mast cell degranulation.
Oka
T
T
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Fujimoto
M
M
Nagasaka
R
R
Ushio
H
H
Hori
M
M
Ozaki
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D015550:Cell Degranulation / Q000187:drug effects*; D003373:Coumaric Acids / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D004140:Dinitrophenols; D004797:Enzyme-Linked Immunosorbent Assay; D006967:Hypersensitivity / Q000097:blood / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000097:blood / Q000188:drug therapy; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D012275:Oryza / Q000737:chemistry; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010666:Phenylpropionates / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D010938:Plant Oils / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017455:Receptors, IgE / Q000378:metabolism; D000073879:Rice Bran Oil; D012709:Serum Albumin; D012867:Skin / Q000187:drug effects
D000888:Antibodies, Anti-Idiotypic; D003373:Coumaric Acids; D004140:Dinitrophenols; D010666:Phenylpropionates; D010936:Plant Extracts; D010938:Plant Oils; D017455:Receptors, IgE; D012709:Serum Albumin; C026408:anti-IgE antibodies; C058064:dinitrophenyl-human serum albumin conjugate; D007073:Immunoglobulin E; C540967:cycloartenyl ferulate; D000073879:Rice Bran Oil; C013172:gamma-oryzanol