title: Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection.
abstract: This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.
authors: Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King's College Road, Toronto, Ontario, Canada. xyliu@mie.utoronto.ca
authors: Xinyu
authors: X
authors: Liu
authors: Yu
authors: Y
authors: Sun
mesh_terms: D000818:Animals; D051379:Mice; D008845:Microinjections / Q000295:instrumentation*; D055616:Microtechnology / Q000295:instrumentation*; D015053:Zygote