An improved method for staining kinetochores of human chromosomes.
An improved method, which exhibited simultaneously clearly kinetochores and the nucleolar organizer regions of human chromosomes by pretreating of human metaphase chromosomes with HCl and NaOH, followed by staining with silver nitrate and visualizing using ammoniacal silver, is described in the present communication. It has important role for analysis of kinetochore variation, mechanism of chromosomal non-disjunction as well as identification of fuctional active centromeres.
He
Junlin
J
Department of Genetics, No.1 Yixueyuan Road, Yuzhong Street, Chongqing 400016, People's Republic of China.
Liu
Xueqing
X
Ding
Yubin
Y
Yu
Chao
C
Weng
Yaguang
Y
Chen
Xuemei
X
Gao
Rufei
R
Wang
Yingxiong
Y
D045744:Cell Line, Tumor; D002877:Chromosomes, Human; D006801:Humans; D018386:Kinetochores; D008677:Metaphase; D013194:Staining and Labeling / Q000379:methods*