[Genetic analysis and mapping of high-tillering and dwarf mutant htd1-2 in rice].
Tillering is one of the most important agronomic traits of rice. In order to explore the molecular mechanism of rice tillering, a high-tillering dwarf 1-2 (htd1-2) mutant was isolated from the offspring of the indica rice (Oryza sativa L. ssp. indica) variety 9311 treated with 350Gy 60Co gamma-radiation. Genetic analysis showed that both high tillering and dwarf phenotypes of htd1-2 were controlled by a single recessive nuclear gene. By means of molecular marker technique, the HIGH-TILLERING DWARF1-2(HTD1-2) gene was mapped between two CAPS markers A6 and E2 on chromosome 4 within 116 kilobases. Within this region, there is a cloned gene HIGH-TILLERING DWARF1(HTD1), which controls rice tillering. By comparing sequences of HTD1 between htd1-2 and 9311, in combination with the results from dCAPS analysis, we believed that HTD1 is an orthologue of the gene HTD1-2. Because of different genetic backgrounds, htd1 and htd1-2 have different phenotypes although they are the allelic mutants. Furthermore, removal of axillary buds proved that dwarfism of htd1-2 mutant is partly attributed to its excessive tillers.
Jiang
Hai-Pai
HP
College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China. haipaijiang@hotmail.com
Zhang
Shu-Ying
SY
Bao
Jin-Song
JS
Wang
Bo-Lun
BL
Wang
Shu
S
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant; D019411:Clinical Laboratory Techniques; D018556:Crops, Agricultural / Q000235:genetics*; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000032:analysis*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D018547:Plant Stems / Q000235:genetics; D017422:Sequence Analysis, DNA
D018744:DNA, Plant