Detection of gastric carcinoma-associated MG7-Ag by serum immuno-PCR assay in a high-risk Chinese population, with implication for screening.
To evaluate gastric carcinoma-associated antigen, MG7-Ag, for detection of gastric cancer in a high-risk population, a population-based screening of gastric cancer was conducted in Linqu County, Shandong Province, China. In 2002 and 2003, a total of 2,710 participants aged 35-65 years received an endoscopic examination with 5 biopsies taken from standard sites with pathological diagnosis, and serum samples were collected to detect MG7-Ag by serum-based Immunopolymerase chain reaction (PCR) assay. The sensitivity and specificity of MG7-Ag Immuno-PCR assay in detecting of gastric cancer were assessed. Of 2,710 participants, 148 (5.46%) were determined to be MG7-Ag positive. The sensitivity of MG7-Ag Immuno-PCR assay for the detection of gastric cancer was 77.5% (31 of 40 gastric cancer cases), the specificity was 95.62% (2,553 of 2,670 nongastric cancer subjects) and the accuracy was 73.12%. A total of 24 gastric cancer cases were in Stage I or II, of which 17 (70.8%) were MG7-Ag positive. However, the proportion of MG7-Ag positivity in subjects with superficial gastritis, chronic atrophic gastritis, intestinal metaplasia, indefinite dysplasia or dysplasia was ranged from 3.00% to 5.61% in comparison with 77.5% in those with gastric cancer. Our findings suggest that MG7-Ag was a sensitive and specific serum biomarker and may have a potential for gastric cancer screening in the high-risk population.
Zhang
Lian
L
Department of Cancer Epidemiology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, China.
Ren
Jun
J
Pan
Kaifeng
K
Ma
Junling
J
Li
Jiyou
J
Shen
Lin
L
Zhang
Xiaodong
X
Li
Jie
J
Fan
Daiming
D
Gail
Mitchell
M
You
Weicheng
W
D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000097:blood; D044466:Asian People; D002681:China / Q000453:epidemiology; D005260:Female; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000208:ethnology / Q000276:immunology
D000951:Antigens, Neoplasm; C508753:MG7 antigen, human