title: Proliferation and osteogenic differentiation of rat bone marrow stromal cells on bioapatite with different crystalline facets.
abstract: The objective of this study is to investigate the attachment, osteogenic differentiation, and collagen matrix secretion of rat bone marrow stromal cells (MSC) and MC3T3-E1 cells on the biological apatite (Ap) with different crystalline facets. The Ap samples with a-facet and c-facet surfaces were prepared by cutting the heat-treated cortical bone of bovine femurs parallel and perpendicular to the longitudinal direction, respectively. MC3T3-E1 cells attached spreading on the a-Ap sample whereas their shape of cells was round on the c-Ap. No difference in the proliferation profile of MSC and MC3T3-E1 cells was observed between the two samples. The activity of alkaline phosphatase was significantly higher for both the cells on the a-Ap sample than that of c-Ap one. The matrix of collagen secreted from MC3T3-E1 cells on the a-Ap sample oriented along the c-axis of Ap, in contrast to those of c-Ap. The cells tended to align along the collagen orientation. It is concluded that the alignment of cells is influenced by the collagen fibril orientation of Ap samples.
authors: Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University, South 1, West 17, Chuo-ku, Sapporo 060-8556.
authors: Norihisa
authors: N
authors: Ichinohe
authors: Takayoshi
authors: T
authors: Nakano
authors: Toshihiro
authors: T
authors: Mitaka
authors: Yukichi
authors: Y
authors: Umakoshi
authors: Yasuhiko
authors: Y
authors: Tabata
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*; D013499:Surface Properties; D019096:Vitronectin / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D005353:Fibronectins; D019096:Vitronectin; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase