Screening and identification of a Streptomyces platensis YK-2, a new transglutaminase producer.
A bacterial strain, YK-2, was isolated as a producer of transglutaminase from a forest soil sample of Daegu, Korea. The isolate showed a G+C content of 72.7 mol%, contained meso-A(2)pm as the cell-wall amino acid, and possessed menaquinone MK-9 (H(6)) and menaquinone MK-9 (H8) at a ratio of 6:4. The chemotaxonomic analysis, as well as phylogenetic analysis based on the 16S rDNA sequence, identified the isolate as a member of Streptomyces platensis. For transglutaminase production, the optimum medium composition was determined to be 2% glucose, 1% polypeptone, 1% soytone, and 0.1% MnCl(2). The transglutaminase was stable within the pH range of 5.0- 9.0 and 30-45 degrees , and the optimum pH and temperature were pH 8.0 and 45 degrees, respectively, without any requirement for Ca(2+).
Yeo
Soo-Hwan
SH
Department of Korean Food Research for Globalization, NAAS, RDA, 160, Nokjiro, Gwonsun-gu, Suwon 441-853, Korea.
Yoon
Jung-Hoon
JH
Lee
Dong Gun
DG
Kim
Hyun Soo
HS
D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D007723:Korea; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D011503:Transglutaminases / Q000096:biosynthesis*; D024482:Vitamin K 2 / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D011503:Transglutaminases