Diffraction imaging of spheres and melanoma cells with a microscope objective.
Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data.
Jacobs
Kenneth M
KM
Department of Physics, East Carolina University, Greenville, NC 27858, USA.
Yang
Li V
LV
Ding
Junhua
J
Ekpenyong
Andrew E
AE
Castellone
Reid
R
Lu
Jun Q
JQ
Hu
Xin-Hua
XH
D000818:Animals; D045744:Cell Line, Tumor; D021621:Imaging, Three-Dimensional; D008027:Light; D008545:Melanoma / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008853:Microscopy; D011137:Polystyrenes / Q000737:chemistry*; D012542:Scattering, Radiation
D011137:Polystyrenes