Production of 3-hydroxypropionaldehyde in silage inoculated with Lactobacillus coryniformis plus glycerol.
We examined the production of an antimicrobial component, 3-hydroxypropionaldehyde (3-HPA), in laboratory-scale silage inoculated with Lactobacillus coryniformis strain 394, which ferments glycerol to 3-HPA. A modified colorimetric method that used an NaOH-treated blank and determined the absorption spectrum of the samples was employed to detect a 3-HPA-like component (HLC) that was assumed to be 3-HPA. Inoculation with Lb. coryniformis 394 plus glycerol in ensiling produced HLC at 10-460 ppm and contributed to inhibition of butyric fermentation and retardation of aerobic spoilage. HLC was considered to be 3-HPA from its absorption spectrum. These results suggest that the production of 3-HPA by Lb. coryniformis 394 is useful in ensiling and that the modified colorimetric method is effective to detect 3-HPA in silage.
Tanaka
Osamu
O
National Agricultural Research Center for Tohoku Region, Morioka, Iwate, Japan. tanakao@affrc.go.jp
Komatsu
Tokushi
T
Oshibe
Akinori
A
Cai
Yimin
Y
Miyazaki
Shigeru
S
Nakanishi
Kotoyoshi
K
D000042:Absorption; D000332:Aerobiosis; D020148:Butyric Acid / Q000378:metabolism; D003124:Colorimetry; D005285:Fermentation; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*; D012275:Oryza; D011407:Propane / Q000378:metabolism*; D012820:Silage / Q000382:microbiology*
D020148:Butyric Acid; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D005990:Glycerol; D011407:Propane