title: Prevalence and management of invalid GeneXpert enterovirus results obtained with cerebrospinal fluid samples: a 2-year study.
abstract: A total of 525 cerebrospinal fluid (CSF) samples submitted during the 2007 and 2008 enteroviral seasons were included in a study to determine the prevalence of and potential risk factors for invalid Cepheid GeneXpert enterovirus assay (GXEA) results, as well as possible solutions for the problem. The invalid GXEA results were reported for 43 (8.2%) specimens and correlated with increased visibility of red blood cells (P < 0.0001) but not with CSF xanthochromia and clotting. Invalid GXEA result rates were markedly diminished by 82.1% and 96.0% and test sensitivities were minimally decreased by 1.7% and 3.6% when these specimens were tested at a 1:5 dilution and after a freeze-thaw cycle, respectively.
authors: Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
authors: Susan E
authors: SE
authors: Sefers
authors: Anna K
authors: AK
authors: Raymer
authors: Jessica T
authors: JT
authors: Kilby
authors: David H
authors: DH
authors: Persing
authors: Yi-Wei
authors: YW
authors: Tang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002555:Cerebrospinal Fluid / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D003951:Diagnostic Errors / Q000517:prevention & control* / Q000706:statistics & numerical data*; D004769:Enterovirus Infections / Q000175:diagnosis*; D005260:Female; D005615:Freezing; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D011933:Reagent Kits, Diagnostic