[Effects of rosiglitazone on expression patterns of the genes involved in adipogenesis during porcine preadipocytes differentiation].
To investigate the effects of rosiglitazone on porcine preadipocytes during the induced differentiation process, we isolated subcutaneous adipose from two-days-old piglets using collagenase-digestion method and cultured preadipocyte cells in the control and experimental medium, respectively. The control group was under the conditions of 10% fetal calf serum in DMEM/F-12 (1:1), insulin (50 nmol/L), dexamethasone (100 nmol/L) and 1-methyl-3-isobutylxanthine (0.25 mmol/L), while the experimental medium was added with 100 nmol/L rosiglitazone. The expression changes of genes associated with adipogenesis between the two different conditions were measured by using qRT-PCR. The results revealed that, in the experimental group, the expression levels of PPARalpha, PPARgamma, C/EBPalpha, FABP4, FASN and GPAT were up-regulated to the maximum at 48 h, 48 h, 48 h, 108 h, 60 h and 24 h after induction, respectively, and the change folds of these genes were 1.7, 48, 3.3, 487.5, 5.8 and 3.6, respectively. While, in the control group, the expression levels of these six genes were up-regulated to the maximum at different time points (84 h, 96 h, 48 h, 96 h, 36 h and 36 h, respectively) after induction, and the change folds of these genes were also different (2.1, 11, 1.6, 216.5, 3.5 and 2.8, respectively). In addition, a good correlation among the expression changes of GPAT and PPARalpha, FASN were all observed at P&0.05 in the experimental group and at P&0.01 in control group. These results indicated that the expression of PPARgamma, C/EBPalpha, FABP4, FASN and GPAT were strongly up-regulated, and the expression of PPARalpha was down-regulated under the effects of rosiglitazon. These results suggest that rosiglitazone has a promotive effect on PPARgamma and C/EBPalpha. Here, we present three tentatively conclusions. First, PPARgamma and C/EBPalpha might be the key transcriptional factors for preadipocytes differentiation. Second, the phospholipids biosynthesis might appear more earlier during the process of adipogenesis. Third, PPARalpha might play an important role in the regulation of phospholipids biosynthesis.
Liu
Hai-Feng
HF
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China. liuhf0-1@126.com
Zhang
Xu
X
Li
Ming-Zhou
MZ
Li
Xue-Wei
XW
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics*; D000818:Animals; D064429:Fatty Acid Synthases / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077154:Rosiglitazone; D013552:Swine / Q000235:genetics* / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology*
D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D005992:Glycerol-3-Phosphate O-Acyltransferase; D064429:Fatty Acid Synthases