The effect of caffeic acid phenethyl ester on the functions of human monocyte-derived dendritic cells.
BACKGROUND
Propolis, an ancient herbal medicine, has been reported the beneficial effect both in asthma patients and murine model of asthma, but the mechanism was not clearly understood. In this study, the effect of caffeic acid phenethyl ester (CAPE), the most extensively studied components in propolis, on the functions of human monocyte-derived dendritic cells (MoDCs) was investigated.


RESULTS
CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract. CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract. In contrast, the upregulation of costimulatory molecules in mature MoDCs was not suppressed by CAPE. Further, the antigen presenting ability of DCs was not inhibited by CAPE. CAPE inhibited IkappaBalpha phosphorylation and NF-kappaB activation but not mitogen-activated protein kinase (MAPK) family phosphorylation in human MoDCs.


CONCLUSIONS
These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway. This study provided a new insight into the mechanism of CAPE in immune response and the rationale for propolis in the treatment of asthma and other allergic disorders.
Wang
Li-Chieh
LC
Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan, Republic of China.lcwang5@ntu.edu.tw
Lin
Yu-Li
YL
Liang
Yu-Chih
YC
Yang
Yao-Hsu
YH
Lee
Jyh-Hong
JH
Yu
Hsin-Hui
HH
Wu
Wen-Mein
WM
Chiang
Bor-Luen
BL
D000485:Allergens / Q000276:immunology; D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D054357:Chemokine CXCL10 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D051550:I-kappa B Kinase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D053762:Interleukin-12 Subunit p40 / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000276:immunology / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects / Q000276:immunology; D011429:Propolis / Q000494:pharmacology*; D039981:Pyroglyphidae / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
D000485:Allergens; C114753:CXCL10 protein, human; D002109:Caffeic Acids; D054357:Chemokine CXCL10; D053762:Interleukin-12 Subunit p40; D008070:Lipopolysaccharides; D016328:NF-kappa B; D016753:Interleukin-10; D018664:Interleukin-12; D011429:Propolis; D051550:I-kappa B Kinase; D020929:Mitogen-Activated Protein Kinase Kinases; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol