Gene-specific and genome-wide ChIP approaches to study plant transcriptional networks.
Chromatin immunoprecipitation (ChIP) provides a versatile tool to investigate the in vivo location of DNA-binding proteins on genomic DNA. ChIP approaches are gaining significance in plants, in cases when entire genome sequences are available (e.g., Arabidopsis), for which several high-density oligo arrays have been or are being developed. Nevertheless, plant ChIP and ChIP-chip still present some technical challenges. Here, we describe general methods for ChIP and ChIP-chip, which have been successfully applied to maize and Arabidopsis.
Morohashi
Kengo
K
Department of Plant Cellular & Molecular Biology and Plant Biotechnology Center, The Ohio State University, Columbus, OH, USA.
Xie
Zidian
Z
Grotewold
Erich
E
D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant; D018745:Genome, Plant / Q000502:physiology; D013379:Substrate Specificity / Q000235:genetics
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins