title: Sex-reversed somatic cell cloning in the mouse.
abstract: Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species.
authors: BioResource Center, RIKEN, Ibaraki, Japan.
authors: Kimiko
authors: K
authors: Inoue
authors: Narumi
authors: N
authors: Ogonuki
authors: Kazuyuki
authors: K
authors: Mekada
authors: Atsushi
authors: A
authors: Yoshiki
authors: Takashi
authors: T
authors: Sado
authors: Atsuo
authors: A
authors: Ogura
mesh_terms: D000367:Age Factors; D000818:Animals; D019976:Cloning, Organism; D012734:Disorders of Sex Development; D004624:Embryo Transfer; D005260:Female; D005836:Genitalia, Female; D005837:Genitalia, Male; D007621:Karyotyping; D008297:Male; D051379:Mice; D053652:Nuclear Transfer Techniques; D012098:Reproduction; D012708:Sertoli Cells / Q000166:cytology* / Q000502:physiology