A novel purification method for multipotential skeletal stem cells.
At least some cells within bone marrow stromal populations are multipotential (i.e., differentiate in vitro into osteoblasts, chondrocytes, and adipocytes) and thus designated skeletal stem cells (SSCs) or mesenchymal stem cells (MSCs) amongst other names. Recently, a subpopulation of stromal cells, notably osteoblasts or their progenitors, has been identified as a definitive regulatory component of the hematopoietic stem cell (HSC) niche. Thus, the development of methods for purifying not only SSCs but cells comprising the HSC niche is of interest. Here, we report a method for purifying a novel bone marrow-derived population with a high frequency of osteoprogenitors and high expression levels of osteoblast differentiation markers (highly purified osteoprogenitors (HipOPs)) as well as markers of the bone niche for HSCs. In vivo transplantation experiments demonstrated that donor HipOPs differentiated into not only osteoblasts, osteocytes and cells around sinusoids but also hematopoietic cells. Thus, HipOPs represent a novel population for simultaneous reconstruction of bone and bone marrow microenvironments.
Itoh
Shousaku
S
Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
Aubin
Jane E
JE
D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D020219:Chondrogenesis / Q000235:genetics; D016001:Confidence Intervals; D005260:Female; D016867:Immunocompromised Host; D018189:Immunomagnetic Separation; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008822:Mice, Transgenic; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D055153:Stem Cell Niche / Q000166:cytology; D055114:X-Ray Microtomography
D000943:Antigens, Differentiation