Protection afforded by a herbal medicine, Sho-seiryu-to (TJ-19), against oleic acid-induced acute lung injury in guinea-pigs.
OBJECTIVE
The effect of a herbal medicine, Sho-seiryu-to (TJ-19), on oleic acid-induced lung injury, an animal model of acute respiratory distress syndrome or acute lung injury (ARDS/ALI), was examined.


METHODS
Acute lung injury was induced by an intravenous injection of 15 microl/kg oleic acid to guinea-pigs. TJ-19 was administered by a single oral dose (3 g/kg) or by multiple oral doses (0.75 g/kg).


RESULTS
The decrease in partial oxygen pressure of arterial blood (Pao(2)) and the increase in airway vascular permeability induced by the oleic acid injection were attenuated by a single dose of TJ-19. When TJ-19 was administered orally twice a day for two weeks and then oleic acid was injected, a potent prophylactic effect of the drug was observed. TJ-19 also prevented airway vascular hyperpermeability, lung cell injury, oxidative stress and thromboxane A(2) generation, associated with the oleic acid injection.


CONCLUSIONS
TJ-19 significantly attenuated the oleic acid-induced lung injury probably through the antioxidative effect and inhibitory effect of thromboxane A(2) generation, although the precise inhibitory mechanisms were not fully elucidated due to the diversity in constituents of the herbal medicine. We suggest that TJ-19 is a promising drug candidate and a medicinal resource for preventing ARDS/ALI.
Yang
Chang-Qing
CQ
Department of Pharmaceutical Sciences, College of Pharmacy, Yanbian University, Yanji, China.
Ishitsuka
Yoichi
Y
Moriuchi
Hiroshi
H
Golbidi
Saeid
S
Jin
Zhen-Ji
ZJ
Irikura
Mitsuru
M
Irie
Tetsumi
T
D055371:Acute Lung Injury / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006168:Guinea Pigs; D029001:Herbal Medicine; D008297:Male; D019301:Oleic Acid; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010946:Plants, Medicinal; D013928:Thromboxane A2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis
D004365:Drugs, Chinese Herbal; C090832:sho-seiryu-to; D019301:Oleic Acid; D013928:Thromboxane A2