Electrospinning of synthesized triblock copolymers of epsilon-caprolactone and L-lactide for the application of vascular tissue engineering.
Biodegradable triblock copolymers of epsilon-caprolactone and L-lactide with varying compositions and molecular weights have been synthesized. They were then used to fabricate compliant small-diameter tissue engineered vascular scaffolds by using an electrospinning technique. The in vitro and in vivo degradation of the ultrafine fabrics was monitored to be faster than their counterpart cast films. A favorable interaction between the scaffolds and the mouse fibroblast L929 cells was demonstrated via MTT assay. A confluent, adherent monolayer of canine mesenchymal stem cells was observed in the tubular scaffold lumen after culture in a bioreactor for 3 days. The scaffold mechanical strength was strong enough to be transplanted into the canine carotid artery.
Qiu
Rong-Xin
RX
School of Materials Science & Engineering, Beijing Institute of Technology, Beijing 100081, People's Republic of China.
Li
Chun-Min
CM
Ye
Lin
L
Dong
Jian-De
JD
Zhang
Ai-Ying
AY
Gu
Yong-Quan
YQ
Feng
Zeng-Guo
ZG
D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D002208:Caproates; D004146:Dioxanes; D007739:L Cells; D007783:Lactones; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D002208:Caproates; D004146:Dioxanes; D007783:Lactones; D011108:Polymers; C121056:caprolactone; C091880:dilactide