title: Peak growth hormone-releasing hormone-arginine-stimulated growth hormone is inversely associated with intramyocellular and intrahepatic lipid content in premenopausal women with obesity.
abstract: BACKGROUND
  Visceral adiposity is a strong determinant of GH secretion, and low endogenous GH secretion is associated with increased insulin resistance, a key component of the metabolic syndrome. Increased fat accumulation in skeletal muscle and liver may play an etiological role in the development of insulin resistance and other complications of the metabolic syndrome. Little is known about the role of decreased endogenous GH secretion in the pathogenesis of insulin resistance in obesity.
  
  
  OBJECTIVE
  To investigate the relationship between intramyocellular lipids (IMCL), intrahepatic lipids, and peak-stimulated GH in premenopausal women with obesity.
  
  
  METHODS
  We conducted a cross-sectional study at a clinical translational research center.
  
  
  METHODS
  Patients included 21 premenopausal women with obesity (mean body mass index, 34.0 +/- 4.5 kg/m(2)) and 17 normal-weight controls (mean body mass index, 21.9 +/- 2.0 kg/m(2)) of comparable mean age.
  
  
  METHODS
  IMCL and intrahepatic lipids were measured with proton magnetic resonance spectroscopy ((1)H-MRS). Body composition was measured with magnetic resonance imaging. Peak GH was measured after stimulation with GHRH-arginine.
  
  
  RESULTS
  Obese subjects had higher IMCL, intrahepatic lipids, abdominal and thigh fat, and thigh muscle mass compared with normal-weight controls. There were strong inverse associations between peak GH and both IMCL and intrahepatic lipids independent of age and visceral adiposity. There were positive associations between IMCL and intrahepatic lipids with measures of insulin resistance and serum triglycerides.
  
  
  CONCLUSIONS
  In premenopausal women with obesity, peak GH is inversely associated with IMCL and intrahepatic lipids independent of age and visceral adiposity. This suggests that low GH may contribute to insulin resistance in obesity through effects on muscle and intrahepatic lipids.
authors: Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA. mbredella@partners.org
authors: Miriam A
authors: MA
authors: Bredella
authors: Martin
authors: M
authors: Torriani
authors: Bijoy J
authors: BJ
authors: Thomas
authors: Reza Hosseini
authors: RH
authors: Ghomi
authors: Danielle J
authors: DJ
authors: Brick
authors: Anu V
authors: AV
authors: Gerweck
authors: Karen K
authors: KK
authors: Miller
mesh_terms: D000328:Adult; D001120:Arginine / Q000378:metabolism*; D001823:Body Composition; D003430:Cross-Sectional Studies; D005260:Female; D013007:Growth Hormone-Releasing Hormone / Q000097:blood / Q000378:metabolism*; D019382:Human Growth Hormone / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007333:Insulin Resistance; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008875:Middle Aged; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism*; D017697:Premenopause; D011522:Protons; D012044:Regression Analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D011522:Protons; D014280:Triglycerides; D019382:Human Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D001120:Arginine