title: Insights into the molecular architecture of the 26S proteasome.
abstract: Cryo-electron microscopy in conjunction with advanced image analysis was used to analyze the structure of the 26S proteasome and to elucidate its variable features. We have been able to outline the boundaries of the ATPase module in the "base" part of the regulatory complex that can vary in its position and orientation relative to the 20S core particle. This variation is consistent with the "wobbling" model that was previously proposed to explain the role of the regulatory complex in opening the gate in the alpha-rings of the core particle. In addition, a variable mass near the mouth of the ATPase ring has been identified as Rpn10, a multiubiquitin receptor, by correlating the electron microscopy data with quantitative mass spectrometry.
authors: Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany.
authors: Stephan
authors: S
authors: Nickell
authors: Florian
authors: F
authors: Beck
authors: Sjors H W
authors: SH
authors: Scheres
authors: Andreas
authors: A
authors: Korinek
authors: Friedrich
authors: F
authors: Förster
authors: Keren
authors: K
authors: Lasker
authors: Oana
authors: O
authors: Mihalache
authors: Na
authors: N
authors: Sun
authors: István
authors: I
authors: Nagy
authors: Andrej
authors: A
authors: Sali
authors: Jürgen M
authors: JM
authors: Plitzko
authors: Jose-Maria
authors: JM
authors: Carazo
authors: Matthias
authors: M
authors: Mann
authors: Wolfgang
authors: W
authors: Baumeister
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D020285:Cryoelectron Microscopy; D004331:Drosophila melanogaster / Q000201:enzymology; D013058:Mass Spectrometry; D008958:Models, Molecular; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry* / Q000648:ultrastructure; D021122:Protein Subunits / Q000737:chemistry; D021381:Protein Transport
chemical_list: D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D000251:Adenosine Triphosphatases