title: Host-dependent roles of the viral 5' untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3' UTR of Red clover necrotic mosaic virus RNA1.
abstract: The genome of Red clover necrotic mosaic virus (RCNMV) consists of RNA1 and RNA2, both lacking a cap structure and a poly(A)tail. RNA1 has a translational enhancer element (3'TE-DR1) in the 3' untranslated region (UTR). In this study, we analyzed the roles of 5' and 3' UTRs of RNA1 in 3'TE-DR1-mediated cap-independent translation in cowpea and tobacco BY-2 protoplasts using a dual-luciferase (Luc) reporter assay system. Most mutations introduced into RNA1 5' UTR in reporter Luc mRNA abolished or greatly reduced cap-independent translation in BY-2 protoplasts, whereas those mutations had no or much milder effects if any on translational activity in cowpea protoplasts. Our results suggest that a stem-loop structure predicted in the 5' proximal region of RNA1 plays important roles in both translation and RNA stability. We also show that 3'TE-DR1-mediated cap-independent translation relies on a ribosome-scanning mechanism in both protoplasts.
authors: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kitashirakawa, Kyoto, 606-8502, Japan.
authors: Siriruk
authors: S
authors: Sarawaneeyaruk
authors: Hiro-Oki
authors: HO
authors: Iwakawa
authors: Hiroyuki
authors: H
authors: Mizumoto
authors: Hiromi
authors: H
authors: Murakami
authors: Masanori
authors: M
authors: Kaido
authors: Kazuyuki
authors: K
authors: Mise
authors: Tetsuro
authors: T
authors: Okuno
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D001483:Base Sequence; D002478:Cells, Cultured; D007887:Fabaceae / Q000821:virology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012315:RNA Caps; D020871:RNA Stability; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000821:virology; D019183:Tombusviridae / Q000235:genetics*; D014764:Viral Proteins
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012315:RNA Caps; D012367:RNA, Viral; D014764:Viral Proteins