Vanadium pentoxide effects on stress responses in wine Saccharomyces cerevisiae strain UE-ME3.
Vanadium pentoxide mainly used as catalyst in sulphuric acid, maleic anhydride and ceramics industry, is a pollutant watering redistributed around the environment. Research on biological influence of vanadium pentoxide has gained major importance because it exerts toxic effects on a wide variety of biological systems. In this work we intent to evaluate the effects of vanadium pentoxide ranging from 0 to 2 mM in culture media on a wine wild-type Saccharomyces cerevisiae from Alentejo region of Portugal. Our results show that 2.0 mM vanadium pentoxide in culture medium induced a significant increase of malonaldehyde level and Glutathione peroxidase activity, a slightly increase of Catalase A activity as well as a decrease of wet weight and mitochondrial NADH cit c reductase of S. cerevisiae UE-ME(3). Also our results show that cycloheximide prevent cell death when cells grows 30 min in presence of 1.5 mM of vanadium pentoxide.
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Tânia
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Departamento de Química, Universidade de Evora, R.Romão Ramalho, 59, 7002-671, Evora, Portugal.
Conim
Ana
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Alves-Pereira
Isabel
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Ferreira
Rui
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D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004785:Environmental Pollutants / Q000633:toxicity*; D005285:Fermentation; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011174:Portugal; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D017968:Vanadium Compounds / Q000633:toxicity*; D014920:Wine / Q000382:microbiology
D004785:Environmental Pollutants; D017968:Vanadium Compounds; D008315:Malondialdehyde; D003513:Cycloheximide; C066075:vanadium pentoxide; D002374:Catalase; D005979:Glutathione Peroxidase