title: An immune responsive complement factor D/adipsin and kallikrein-like serine protease (PoDAK) from the olive flounder Paralichthys olivaceus.
abstract: The cDNA encoding of a complement factor D/adipsin and kallikrein-like serine protease, designated PoDAK, was isolated from the olive flounder Paralichthys olivaceus. PoDAK cDNA encodes a polypeptide with 277 amino acids containing conserved catalytic triad residues of serine proteases. The amino acid sequence of PoDAK showed high similarity to the kallikrein-like protein of medaka, mammalian adipsin/complement factor D and tissue kallikrein homolog, KT-14 of trout, complement factor D of zebrafish, and shared 31.6-36.8% homology with complement factor D/adipsin known from other species, including mammals. Phylogenetic analysis revealed that PoDAK clustered with the kallikrein-like protein of medaka and mammalian adipsin/complement factor D and tissue kallikrein homolog KT-14 of trout. The expression of PoDAK mRNA was high in the gills and heart, moderate in muscle, liver, intestine, stomach, kidney, and spleen of healthy flounder, and increased in the kidney, liver, and spleen of flounder challenged by the viral hemorrhagic septicemia virus (VHSV) or Streptococcus iniae. In situ hybridization confirmed that PoDAK mRNA is localized in the kidney and heart of individuals infected with VHSV. Further investigations are needed to clarify the function of PoDAK in vivo and in vitro.
authors: Biotechnology Research Division, National Fisheries Research and Development Institute, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan 619-705, Republic of Korea. konghj@nfrdi.go.kr
authors: Hee Jeong
authors: HJ
authors: Kong
authors: Gyeong-Eun
authors: GE
authors: Hong
authors: Bo-Hye
authors: BH
authors: Nam
authors: Young-Ok
authors: YO
authors: Kim
authors: Woo-Jin
authors: WJ
authors: Kim
authors: Sang-Jun
authors: SJ
authors: Lee
authors: Nam-Sil
authors: NS
authors: Lee
authors: Jeong Wan
authors: JW
authors: Do
authors: Hyun Kook
authors: HK
authors: Cho
authors: Jaehun
authors: J
authors: Cheong
authors: Chang Hoon
authors: CH
authors: Lee
authors: Kyung-Kil
authors: KK
authors: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D011416:Complement Factor D / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D005393:Fish Diseases / Q000201:enzymology / Q000276:immunology; D005432:Flounder / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007610:Kallikreins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D029141:Novirhabdovirus; D010802:Phylogeny; D012333:RNA, Messenger / Q000276:immunology; D018353:Rhabdoviridae Infections / Q000201:enzymology / Q000276:immunology / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D012333:RNA, Messenger; D007610:Kallikreins; D011416:Complement Factor D