Ultrastructure of human fetal sympathetic ganglia after permanganate fixation - absence of granular vesicles.
Midterm human fetal sympathetic ganglia were studied using the formaldehyde-induced fluorescence technique for demonstrating catecholamines, and after glutaraldehyde-OsO(4) or permanganate fixation for electron microscopy. The fluorescence technique indicated the presence of catecholamines in most of the developing neurons. After glutaraldehyde-OsO(4) fixation large granular vesicles were found in the perikarya, but a more specific technique for catecholamines, permanganate fixation, revealed no granular vesicles at this stage of development. This indicates that most of the catecholamines are loosely bound in a non-granular store during development.
Hervonen
A
A
Department of Biomedical Sciences, University of Tampere, Tampere, Finland.
Kanerva
L
L
Hervonen
H
H