title: Chromosomal imbalances, 11q21 rearrangement and MECT1-MAML2 fusion transcript in mucoepidermoid carcinomas of the salivary gland.
abstract: The aim of this study was to determine genetic alterations in mucoepidermoid carcinomas of the salivary gland in association with clinical and histopathological parameters. Nineteen formalin-fixed, paraffin-embedded tumors were analysed by using comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH) on interphase nuclei and reverse transcriptase-polymerase chain reaction (RT-PCR) for detection of MECT1-MAML2 fusion transcript. The CGH analysis showed an overrepresentation of chromosome X and losses of entire chromosomes or regions on chromosome 1, 2, and 15 as the most frequent copy number changes. In 37% of the analysed tumors a MAML2-rearrangement by interphase FISH was detected, whereas 58% of the samples showed expression of MECT1-MAML2 fusion transcript. We conclude that the presence of MAML2-rearrangement as well as of MECT1-MAML2 fusion transcript may reflect a more favourable prognosis and may be a useful marker for clinical prediction of the biological behavior of these tumors as previously reported.
authors: Institute of Pathology, Medical University of Innsbruck, 6020 Innsbruck, Austria. irmgard.verdorfer@i-med.ac.at
authors: Irmgard
authors: I
authors: Verdorfer
authors: Andre
authors: A
authors: Fehr
authors: Jörn
authors: J
authors: Bullerdiek
authors: Nina
authors: N
authors: Scholz
authors: Andrea
authors: A
authors: Brunner
authors: Jens
authors: J
authors: Krugmann
authors: Martina
authors: M
authors: Hager
authors: Heike
authors: H
authors: Haufe
authors: Gregor
authors: G
authors: Mikuz
authors: Arne
authors: A
authors: Scholtz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018277:Carcinoma, Mucoepidermoid / Q000235:genetics*; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D006801:Humans; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012468:Salivary Gland Neoplasms / Q000235:genetics*
chemical_list: C495418:MECT1-MAML2 fusion protein, human; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger