title: [Sample preprocessing method for residual quinolones in honey using immunoaffinity resin].
abstract: A sample preparation method was developed for determination of quinolones in honey using immunoaffinity resin. For this purpose, an immunoaffinity resin for quinolones was prepared by coupling a quinolone-specific monoclonal antibody to agarose resin. Honey samples diluted with phosphate buffer were reacted with immunoaffinity resin. After the resin was washed, quinolones were eluted with glycine-HCl. Quinolones in the eluate were determined by HPLC with fluorescence detection. No interfering peak was found on the chromatograms of honey samples. The recoveries of quinolones from samples were over 70% at fortification levels of 20 ng/g (for norfloxacin, ciprofloxacin and enrofloxacin) and 10 ng/g (for danofloxacin). The quantification limits of quinolones were 2 ng/g. This sample preprocessing method using immunoaffinity resin was found to be effective and suitable for determining residual quinolones in honey.
authors: Frontier Institute Co., Ltd, Ishikari, Hokkaido 0613241, Japan.
authors: Yoshiharu
authors: Y
authors: Ihara
authors: Mihoko
authors: M
authors: Kato
authors: Tsukasa
authors: T
authors: Kodaira
authors: Shinji
authors: S
authors: Itoh
authors: Mika
authors: M
authors: Terakawa
authors: Masakazu
authors: M
authors: Horie
authors: Koichi
authors: K
authors: Saito
authors: Hiroyuki
authors: H
authors: Nakazawa
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods*; D006722:Honey / Q000032:analysis*; D015363:Quinolones / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones