Towards the elucidation of molecular determinants of cooperativity in the liver bile acid binding protein.
Bile acid binding proteins (BABPs) are cytosolic lipid chaperones contributing to the maintenance of bile acid homeostasis and functional distribution within the cell. Liver BABPs act in parallel with ileal transporters to ensure vectorial transport of bile salts in hepatocytes and enterocytes, respectively. We describe the investigation of ligand binding to liver BABP, an essential step in the understanding of intracellular bile salt transport. Binding site occupancies were monitored in NMR titration experiments using (15)N-labelled ligand, while the relative populations of differently bound BABP forms were assessed by mass spectrometry. This site-specific information allowed the determination of intrinsic thermodynamic parameters and the identification of an extremely high cooperativity between two binding sites. Protein-observed NMR experiments revealed a global structural rearrangement which suggests an allosteric mechanism at the basis of the observed cooperativity. The view of a molecular tool capable of buffering against significant concentrations of free bile salts in a large range of solution conditions emerges from the observed pH-dependence of binding. We set to determine the molecular determinants of cooperativity by analysing the binding properties of a protein containing a mutated internal histidine. Both mass spectrometry and NMR experiments are consistent with an overall decreased binding affinity of the mutant, while the measured diffusion coefficients of ligand species reveal that the affinity loss concerns essentially one of the two binding sites. We therefore identified a mutation able to disrupt energetic communication functional to efficient binding and conclude that the buried histidine establishes contacts that stabilize the ternary complex.
Pedò
Massimo
M
Dipartimento di Biotecnologie, Università di Verona, Strada le Grazie 15, Verona 37134, Italy.
D'Onofrio
Mariapina
M
Ferranti
Pasquale
P
Molinari
Henriette
H
Assfalg
Michael
M
D000495:Allosteric Site; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry*; D002645:Chickens; D004058:Diffusion; D006639:Histidine / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008562:Membrane Glycoproteins / Q000737:chemistry*; D009154:Mutation; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry
D002352:Carrier Proteins; D008024:Ligands; D008562:Membrane Glycoproteins; D011506:Proteins; C037643:bile acid binding proteins; D006639:Histidine; D006859:Hydrogen