Long lasting immunity in chickens induced by a single shot of influenza vaccine prepared from inactivated non-pathogenic H5N1 virus particles against challenge with a highly pathogenic avian influenza virus.
An influenza vaccine was prepared from inactivated whole particles of the non-pathogenic strain A/duck/Hokkaido/Vac-1/04 (H5N1) virus using an oil adjuvant containing anhydromannitol-octadecenoate-ether (AMOE). The vaccine was injected intramuscularly into five 4-week-old chickens, and 138 weeks after vaccination, they were challenged intranasally with 100 times 50% chicken lethal dose of the highly pathogenic avian influenza (HPAI) virus A/chicken/Yamaguchi/7/04 (H5N1). All 5 chickens survived without exhibiting clinical signs of influenza, although 2 days post-challenge, 3 vaccinated chickens shed limited titres of viruses in laryngopharyngeal swabs.
Sasaki
Takashi
T
Avian Biologics Department, Kyoto Biken Laboratories, Inc., 24-16 Makishima-cho, Uji, Kyoto 611-0041, Japan. keibyou_one@kyotobiken.co.jp
Kokumai
Norihide
N
Ohgitani
Toshiaki
T
Sakamoto
Ryuichi
R
Takikawa
Noriyasu
N
Lin
Zhifeng
Z
Okamatsu
Masatoshi
M
Sakoda
Yoshihiro
Y
Kida
Hiroshi
H
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens / Q000276:immunology* / Q000821:virology; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control*; D016865:Reassortant Viruses / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007252:Influenza Vaccines