[The expression analysis of Grb10 during mouse embryonic development].
Grb10 gene expression was analyzed in fetus and four organs during gestation process using the real-time RT-PCR and in situ hybridization. The real-time RT-PCR analysis result showed that the expression of Grb10 in whole embryos is throughout E8.5 to E19.5. The Grb10 gene expression level was gradually increased from E8.5 to E13.5, and then gradually reduced. The tissues specific expression levels were decreased in brain, heart, and lung from E12.5 to E19.5, but a peak expression was observed in liver at E18.5. Grb10 gene expression was also investigated at E13.5, E15.5, E16.5 and E18.5 sections by using in situ hybridization analysis. Strong signals of Grb10 were detected in brain, spine, kidney, and muscle tissues. These results suggest that Grb10 may play an important role during the development process in mouse.
Liu
Qi
Q
Developmental Biology Lab, Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China. liuqi.ssc@2004.hit.edu.cn
Wang
Yan
Y
Chen
Yan
Y
Zhang
Feng-Wei
FW
Gu
Tian-Tian
TT
Qu
You-Peng
YP
Yue
Lei
L
Wu
Qiong
Q
D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D051383:GRB10 Adaptor Protein / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice / Q000235:genetics* / Q000254:growth & development*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051383:GRB10 Adaptor Protein