Febrile seizures: characterization of double-stranded RNA-induced gene expression.
An association has long been suspected between febrile seizures and interleukin-1beta, the most potent endogenous pyrogen. Interleukin-1beta production increases after double-stranded RNA stimulation in leukocytes of febrile seizure patients. To elucidate the genetics of the immune response, the gene expression pattern after double-stranded RNA stimulation was investigated using DNA microarray. Compared with the control group, expression of the genes ACCN4 (sodium channel), KCNC3 (potassium channel), GABRE (gamma-aminobutyric acid receptor epsilon subunit), RIPK2 (receptor interacting protein kinase-2), TLR4 (toll-like receptor-4), IL26 (interleukin-26), and TNF (tumor necrosis factor), and CASP1 (caspase-1) was increased in the febrile seizure group (P < 0.01). Because RIPK2 and CASP1 are associated with interleukin-1beta production, increased expression might cause increased interleukin-1beta production in the febrile seizure patients. The induced expression of several ion channel genes by double-stranded RNA may affect neuronal excitability which leads to seizure susceptibility during infection.
Sasaki
Kazuya
K
Department of Pediatrics, Faculty of Medicine, Saga University, Saga, Japan.
Matsuo
Muneaki
M
Maeda
Toshiyuki
T
Zaitsu
Masafumi
M
Hamasaki
Yuhei
Y
D062565:Acid Sensing Ion Channels; D020170:Caspase 1 / Q000235:genetics / Q000378:metabolism; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012330:RNA, Double-Stranded / Q000378:metabolism*; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000235:genetics / Q000378:metabolism; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D003294:Seizures, Febrile / Q000235:genetics*; D051670:Shaw Potassium Channels / Q000235:genetics / Q000378:metabolism; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
C568270:ASIC4 protein, human; D062565:Acid Sensing Ion Channels; C546274:GABRE protein, human; C406767:IL26 protein, human; D007378:Interleukins; C489594:KCNC3 protein, human; D009419:Nerve Tissue Proteins; D012330:RNA, Double-Stranded; D011963:Receptors, GABA-A; D051670:Shaw Potassium Channels; D015222:Sodium Channels; C495345:TLR4 protein, human; C439524:TNF protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C506253:RIPK2 protein, human; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D020170:Caspase 1