Adipose-derived stem cells are an effective cell candidate for treatment of heart failure: an MR imaging study of rat hearts.
This study assessed the potential therapeutic efficacy of adipose-derived stem cells (ASCs) on infarcted hearts. Myocardial infarction was induced in rat hearts by occlusion of the left anterior descending artery (LAD). One week after LAD occlusion, the rats were divided into three groups and subjected to transplantation of ASCs or transplantation of cell culture medium (CCM) or remained untreated. During a 1-mo recovery period, magnetic resonance imaging showed that the ASC-treated hearts had a significantly greater left ventricular (LV) ejection fraction and LV wall thickening than did the CCM-treated and untreated hearts. The capillary density in infarct border zone was significantly higher in the ASC-treated hearts than in the CCM-treated and untreated hearts. However, only 0.5% of the ASCs recovered from the ASC-treated hearts were stained positive for cardiac-specific fibril proteins. It was also found that ASCs under a normal culture condition secreted three cardiac protective growth factors: vascular endothelial growth factor, hepatocyte growth factor, and insulin-like growth factor-1. Results of this study suggest that ASCs were able to improve cardiac function of infarcted rat hearts. Paracrine effect may be the mechanism underlying the improved cardiac function and increased capillary density.
Wang
Lei
L
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Canada.
Deng
Jixian
J
Tian
Weichen
W
Xiang
Bo
B
Yang
Tonghua
T
Li
Gang
G
Wang
Jian
J
Gruwel
Marco
M
Kashour
Tarek
T
Rendell
John
J
Glogowski
Miriam
M
Tomanek
Boguslaw
B
Freed
Darren
D
Deslauriers
Roxanne
R
Arora
Rakesh C
RC
Tian
Ganghong
G
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002196:Capillaries / Q000502:physiology; D002454:Cell Differentiation; D003326:Coronary Circulation / Q000502:physiology; D004195:Disease Models, Animal; D005260:Female; D006333:Heart Failure / Q000473:pathology / Q000517:prevention & control / Q000628:therapy*; D017228:Hepatocyte Growth Factor / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008279:Magnetic Resonance Imaging; D008565:Membrane Proteins / Q000378:metabolism; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D050151:Subcutaneous Fat / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling / Q000502:physiology
D015415:Biomarkers; D008565:Membrane Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I