title: Ionic dissolution products of NovaBone promote osteoblastic proliferation via influences on the cell cycle.
abstract: This study investigated the effects of the ionic dissolution products of NovaBone on osteoblastic proliferation and cell cycle regulation. MG63 osteoblast-like cells were cultured in NovaBone-conditioned Dulbecco's Modified Eagle's Medium (DMEM) or control DMEM for 10 days. The concentration of silicon ions was significantly higher in NovaBone-conditioned DMEM than control DMEM. MG63 cells cultured in NovaBone -conditioned DMEM exhibited greater proliferation on days 1 and 4 than control cells. There were increased proportions of Novabone-conditioned DMEM-cultured cells in the S and G(2)/M phases, and decreased proportions in the G(0)/G(1) phase on days 1 and 4 versus control cells, while no differences were observed on days 7 and 10 between the two groups. Bone morphogenic protein 2 production increased in both groups, but was significantly higher for the NovaBone-conditioned DMEM-cultured cells on day 10 compared with the controls. In conclusion, the NovaBone ionic dissolution products, particularly the silicon ions, promoted proliferation of MG63 osteoblast-like cells in vitro via influences on the cell cycle.
authors: Orthopaedic Department, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
authors: Z
authors: Z
authors: Qiu
authors: H
authors: H
authors: Yang
authors: J
authors: J
authors: Wu
authors: L
authors: L
authors: Wei
authors: J
authors: J
authors: Li
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry* / Q000494:pharmacology*; D017077:Culture Media, Conditioned; D007477:Ions; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D012995:Solubility / Q000187:drug effects
chemical_list: C022107:Bioglass; D055396:Bone Morphogenetic Protein 2; D017077:Culture Media, Conditioned; D007477:Ions