A unique micromechanocalorimeter for simultaneous measurement of heat rate and force production of cardiac trabeculae carneae.
To study cardiac muscle energetics quantitatively, it is of paramount importance to measure, simultaneously, mechanical and thermal performance. Ideally, this should be achieved under conditions that minimize the risk of tissue anoxia, especially under high rates of energy expenditure. In vitro, this consideration necessitates the use of preparations of small radial dimensions. To that end, we have constructed a unique micromechanocalorimeter, consisting of an open-ended flow-through microcalorimeter, a force transducer, and a pair of muscle-length actuators. The device enables the metabolic and mechanical performance of cardiac trabeculae carneae to be investigated for prolonged periods in a continuously replenished oxygen- and nutrient-rich environment.
Han
June-Chiew
JC
Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.
Taberner
Andrew J
AJ
Kirton
Robert S
RS
Nielsen
Poul M
PM
Smith
Nicholas P
NP
Loiselle
Denis S
DS
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D002138:Calibration; D002151:Calorimetry / Q000295:instrumentation*; D004558:Electric Stimulation; D004734:Energy Metabolism / Q000502:physiology; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010210:Papillary Muscles / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014475:Uncoupling Agents / Q000494:pharmacology
D014475:Uncoupling Agents; D019297:2,4-Dinitrophenol; D002118:Calcium