Complete genome sequence of a virulent Newcastle disease virus  isolated from an outbreak in chickens in Egypt.
The complete genome sequence of a virulent Newcastle disease virus (NDV) isolated from chickens in Egypt was determined and compared to the sequence of NDV strains isolated from different parts of the world. The genome is 15,186 nucleotides (nt) long and consists of 6 genes in the order of 3'-N-P-M-F-HN-L-5'. The genome contains a 55-nt leader region at the 3' end and a 114-nt trailer region at the 5' end. Interestingly, the phylogenetic analysis showed that strain Egypt is closely related with the NDV strains isolated in China. In addition, the sequence of the fusion protein cleavage site of strain Egypt was identical to that of the NDV strain recently isolated in Mali. Determination of complete genome sequences of additional NDV strains from Africa is necessary to understand the epidemiology of currently circulating viruses in Africa.
Mohamed
Mahmoud H A
MH
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA.
Kumar
Sachin
S
Paldurai
Anandan
A
Megahed
Mohamed M
MM
Ghanem
Ibrahim A
IA
Lebdah
Mohamed A
MA
Samal
Siba K
SK
D000818:Animals; D002645:Chickens / Q000821:virology; D016000:Cluster Analysis; D004196:Disease Outbreaks; D004534:Egypt / Q000453:epidemiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology* / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D012367:RNA, Viral