Orally administered Lactobacillus paracasei KW3110 induces in vivo IL-12 production.
Lactic acid bacteria (LAB) are popularly used as probiotics, and some strains of LAB have anti-allergic functions in vivo. Although in vitro studies show that LAB modulate the T helper type (Th) 1/Th2 balance and inhibit IgE secretion by inducing IL-12, it is not known how LAB regulates allergies in vivo. In this study, we evaluated in vivo IL-12 production after oral administration of Lactobacillus paracasei KW3110, a strain reported to improve allergies, to mice. Orally administered KW3110 interacted with CD11b positive cells and induced IL-12 mRNA expression at Peyer's patch. In addition, blood IL-12 levels increased transiently 10 h after administration of KW3110. Based on these results, we found that oral administration of KW3110 induces IL-12 in vivo. Our findings should contribute to understanding of the in vivo function of LAB.
Ichikawa
Shintaro
S
Central Laboratories for Frontier Technology, Kirin Holdings Co, Ltd, Yokohama, Japan. Shintaro_Ichikawa@kirin.co.jp
Miyake
Mika
M
Fujii
Rei
R
Konishi
Yutaka
Y
D000284:Administration, Oral; D000818:Animals; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005786:Gene Expression Regulation; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology / Q000378:metabolism; D007778:Lactobacillus / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage* / Q000378:metabolism; D013194:Staining and Labeling; D018417:Th1 Cells / Q000276:immunology; D013997:Time Factors
D018664:Interleukin-12; D016650:Fluorescein-5-isothiocyanate