Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555.
The lipopolysaccharide of Citrobacter freundii O22 (strain PCM 1555) was degraded under mild acidic conditions and the O-polysaccharide released was isolated by gel chromatography. Sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H ROESY and (1)H,(13)C HMBC experiments, showed that the repeating unit of the O-polysaccharide has the following structure: alpha-Abep 1 -->3 --> 2)-alpha-D-Manp-(1-->4)-alpha-L-Rhap-(1-->3)-alpha-D-Galp-(1--> where Abe is abequose (3,6-dideoxy-D-xylo-hexose). SDS-PAGE and immunoblotting revealed that the O-antigen of C. freundii O22 is serologically indistinguishable from those of Salmonella group B serovars (Typhimurium, Brandenburg, Sandiego, Paratyphi B) but not related to other abequose-containing O-antigens tested (Citrobacter werkmanii O38 and Salmonella Kentucky) or colitose (l enantiomer of abequose)-containing O-antigen of Escherichia coli O111.
Katzenellenbogen
Ewa
E
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland. katzenel@iitd.pan.wroc.pl
Kocharova
Nina A
NA
Toukach
Philip V
PV
Górska
Sabina
S
Korzeniowska-Kowal
Agnieszka
A
Bogulska
Maria
M
Gamian
Andrzej
A
Knirel
Yuriy A
YA
D002240:Carbohydrate Sequence; D016971:Citrobacter freundii / Q000737:chemistry* / Q000145:classification; D004591:Electrophoresis, Polyacrylamide Gel; D006601:Hexoses / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D012475:Salmonella / Q000737:chemistry
D006601:Hexoses; D019081:O Antigens; C023708:abequose