title: Ranavirus in wild edible frogs Pelophylax kl. esculentus in Denmark.
abstract: A survey for the amphibian pathogens ranavirus and Batrachochytrium dendrobatidis (Bd) was conducted in Denmark during August and September 2008. The public was encouraged via the media to register unusual mortalities in a web-based survey. All members of the public that registered cases were interviewed by phone and 10 cases were examined on suspicion of disease-induced mortality. All samples were negative for Bd. Ranavirus was isolated from 2 samples of recently dead frogs collected during a mass mortality event in an artificial pond near Slagelse, Denmark. The identity of the virus was confirmed by immunofluorescent antibody test. Sequencing of the major capsid protein gene showed the isolate had more than 97.3% nucleotide homology to 6 other ranaviruses.
authors: National Veterinary Institute, Technical University of Denmark, Hangøvej 2, 8200 Arhus N, Denmark. ellen.ariel@jcu.edu.au
authors: Ellen
authors: E
authors: Ariel
authors: Jos
authors: J
authors: Kielgast
authors: Hans Erik
authors: HE
authors: Svart
authors: Knud
authors: K
authors: Larsen
authors: Hannele
authors: H
authors: Tapiovaara
authors: Britt Bang
authors: BB
authors: Jensen
authors: Riikka
authors: R
authors: Holopainen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D004266:DNA Virus Infections / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D003718:Denmark / Q000453:epidemiology; D008969:Molecular Sequence Data; D017995:Ranavirus / Q000502:physiology*; D011898:Ranidae / Q000821:virology*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D036022:Capsid Proteins