High molecular weight blood group A trisaccharide-polyacrylamide glycoconjugates as synthetic blood group A antigens for anti-A antibody removal devices.
Specific immunoadsorption of blood group antibodies by synthetic antigens immobilized on support matrices in the peri-transplantation period provides a promising solution to hyperacute rejection risk following ABO-incompatible transplantation. In this study, we investigated binding interactions between anti-A antibodies and synthetic blood group A trisaccharide conjugated with polyacrylamide of different molecular weights (30 and 1000 kDa). The glycopolymers were equipped with biotin tags and deposited on streptavidin-coated sensor chips. The affinity and kinetics of anti-A antibodies binding to glycoconjugates were studied using surface plasmon resonance (SPR). The high molecular weight conjugate (Atri-PAA(1000)-biotin) enhanced antibody binding capacity by two to three fold compared with the low molecular weight conjugate (Atri-PAA(30)-biotin), whereas varying the carbohydrate content in Atri-PAA(1000)-biotin (20 mol % or 50 mol %) did not affect antibody binding capacity of the glycoconjugate. The obtained results suggest that immunoadsorption devices, especially hollow fiber-based antibody filters which are limited in available surface area for antigen immobilization, may greatly benefit from the new synthetic high molecular weight polyacrylamide glycoconjugates.
Alikhani
Azadeh
A
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15203.|Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15203.
Korchagina
Elena Y
EY
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Chinarev
Alexander A
AA
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Bovin
Nicolai V
NV
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Federspiel
William J
WJ
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15203.|Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15203.|Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15203.|Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15203.
D000180:Acrylic Resins / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D001710:Biotin; D001789:Blood Group Antigens / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D007120:Immunochemistry; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D007202:Indicators and Reagents; D007700:Kinetics; D008970:Molecular Weight; D014312:Trisaccharides / Q000737:chemistry*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D001710:Biotin; D001789:Blood Group Antigens; D007075:Immunoglobulin M; D007202:Indicators and Reagents; D014312:Trisaccharides; D000099166:Polyacrylamides