Determination of the antifungal agent posaconazole in human serum by HPLC with parallel column-switching technique.
Posaconazole is a new broad-spectrum antifungal agent that is currently only available as an oral suspension and shows high intra- and inter-individual differences in oral bioavailibility. Pre-existing methods for the determination of the substance involve the use of internal standards or require a quite complicated and time-consuming sample pre-treatment. Our HPLC method is fast and fully-automated and there is no need for any manual sample pre-treatment. On-line transfer of posaconazole from the extraction column was followed by chromatographic separation on a C18 column and fluorescence detection (lambda(ex): 261 nm, lambda(em): 357 nm). Retention time of posaconazole was about 11.7 min, the lower limit of quantification was found to be 0.1 mg/l. A linear calibration curve was obtained over the concentration range of 0.1-5 mg/l using a 50 microl sample (r(2)=0.999). The relative standard deviations of intra-day variations ranged from 2.3% to 9.4%, intra-day accuracy from 88.8% to 114.8%.
Neubauer
Werner
W
Department of Hematology & Oncology, University Medical Center Freiburg, Freiburg, Germany.
König
Armin
A
Bolek
Richard
R
Trittler
Rainer
R
Engelhardt
Monika
M
Jung
Manfred
M
Kümmerer
Klaus
K
D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D014230:Triazoles / Q000097:blood*
D000935:Antifungal Agents; D014230:Triazoles; C101425:posaconazole