Growth inhibition and cell cycle arrest in the G0/G1 by schizandrin, a dibenzocyclooctadiene lignan isolated from Schisandra chinensis, on T47D human breast cancer cells.
Schizandrin is one of the main dibenzocyclooctadiene lignans present in the fruit of Schisandra chinensis (Schisandraceae). Biological activities including hepatoprotective, antiviral and neuroprotective effects of schizandrin and other dibenzocyclooctadiene lignans have been reported previously. However, the antiproliferative effect of schizandrin against human cancer cells has been poorly determined to date. This study examined the antiproliferative effect of schizandrin in human breast cancer cells. Schizandrin exhibited growth inhibitory activities in cultured human breast cancer cells, and the effect was the more profound in estrogen receptor (ER)-positive T47D cells than in ER-negative MDA-MB-231 cells. When treated with the compound in T47D cells, schizandrin induced the accumulation of a cell population in the G0/G1 phase, which was further demonstrated by the induction of CDK inhibitors p21 and p27 and the inhibition of the expression of cell cycle checkpoint proteins including cyclin D1, cyclin A, CDK2 and CDK4. These results suggest that schizandrin inhibits cell proliferation through the induction of cell cycle arrest with modulating cell cycle-related proteins in human breast cancer cells.
Kim
Sun-Jack
SJ
College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul, Korea.
Min
Hye-Young
HY
Lee
Eun Jin
EJ
Kim
Yeong Shik
YS
Bae
KiHwan
K
Kang
Sam Sik
SS
Lee
Sang Kook
SK
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000378:metabolism; D034242:Cyclooctanes / Q000494:pharmacology*; D005260:Female; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D015394:Molecular Structure; D011083:Polycyclic Compounds / Q000494:pharmacology*; D032141:Schisandra / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C510994:dibenzocyclooctadiene lignan; C011105:schizandrin