title: Analysis of glucose-dependent insulinotropic peptide receptor (GIPR) and luteinizing hormone receptor (LHCGR) expression in human adrenocortical hyperplasia.
abstract: OBJECTIVE
  To analyze the aberrant expression of the GIPR and LHCGR in different forms of adrenocortical hyperplasia: ACTH-independent macronodular adrenal hyperplasia (AIMAH), primary pigmented nodular adrenocortical disease (PPNAD) and diffuse adrenal hyperplasia secondary to Cushing's disease (DAHCD).
  
  
  METHODS
  We quantified GIPR and LHCGR expressions using real time PCR in 20 patients with adrenocortical hyperplasia (seven with AIMAH, five with PPNAD, and eight with DAHCD). Normal adrenals tissues were used as control and the relative expression was compared with beta-actin.
  
  
  RESULTS
  GIPR and LHCGR expressions were demonstrated in all tissues studied. Median GIPR and LHCGR mRNA levels were 1.6; 0.4; 0.5 and 1.3; 0.9; 1.0 in adrenocortical tissues from AIMAH, PPNAD and DAHCD respectively. There were no differences between GIPR and LHCGR expressions in all tissues studied.
  
  
  CONCLUSIONS
  GIPR and LHCGR overexpression were not identified in the studied cases, thus suggesting that this molecular mechanism is not involved in adrenocortical hyperplasia in our patients.
authors: Unidade de Endocrinologia do Desenvolvimento, Laboratório de Hormônios e Genética Molecular LIM/42, Divisão de Endocrinologia e Metabologia, São Paulo, SP, Brazil. mhsc@usp.br
authors: Marcia Helena Soares
authors: MH
authors: Costa
authors: Sorahia
authors: S
authors: Domenice
authors: Ana Claudia
authors: AC
authors: Latronico
authors: Regina Matsunaga
authors: RM
authors: Martin
authors: Mirian Yumie
authors: MY
authors: Nishi
authors: Antonio Marmo
authors: AM
authors: Lucon
authors: Berenice Bilharinho
authors: BB
authors: Mendonca
authors: Maria Candida Barisson Villares
authors: MC
authors: Fragoso
mesh_terms: D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000303:Adrenal Cortex Diseases / Q000235:genetics / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D047748:Pituitary ACTH Hypersecretion / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011964:Receptors, Gastrointestinal Hormone / Q000235:genetics / Q000378:metabolism*; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D000199:Actins; D012333:RNA, Messenger; D011964:Receptors, Gastrointestinal Hormone; D011974:Receptors, LH; C041086:gastric inhibitory polypeptide receptor