In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering.
During the last decades, different materials of both natural and synthetic origin have been developed with the aim of inducing and controlling osteogenic differentiation of mesenchymal stem cells (MSCs). In order for that to happen, it is necessary that the material to be implanted obey a series of requirements, namely: osteoconduction, biocompatibility, and biodegradability. Additionally, they must be low-priced, easy to produce, shape, and store. Hydroxyapatite (HA) is a well known ceramic with a composition similar to the mineral component of bone and is highly biocompatible and easy to obtain and/or process. On the other hand, collagen is the main structural protein present in the human body and bone. In this study, a polymer replication method was applied and a highly porous HA scaffold was produced. Collagen was later incorporated to improve the biological properties of the scaffold while resembling the bone composition. The scaffolds were characterized by means of scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive spectroscopy. In vitro and in vivo testing was performed in all scaffolds produced. The goal of this study was to evaluate the in vivo osteogenic potential of MSCs from two different species seeded on the different HA basedporous scaffolds with collagen type I. The resultsindicate that all scaffolds exhibit relevant bone formation, being more prominent in the case of the HA scaffolds.
Teixeira
S
S
INEB-Instituto de Engenharia Biomédica, Laboratório de Biomaterials, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal. sandra_teixeira3@yahoo.com
Fernandes
H
H
Leusink
A
A
van Blitterswijk
C
C
Ferraz
M P
MP
Monteiro
F J
FJ
de Boer
J
J
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D017886:Durapatite