title: Phosphorylation of native porcine olfactory binding proteins.
abstract: The identification of various isoforms of olfactory binding proteins is of major importance to elucidate their involvement in detection of pheromones and other odors. Here, we report the characterization of the phosphorylation of OBP (odorant binding protein) and Von Ebner's gland protein (VEG) from the pig, Sus scrofa. After labeling with specific antibodies raised against the three types of phosphorylation (Ser, Thr, Tyr), the phosphate-modified residues were mapped by using the beta-elimination followed by Michael addition of dithiothreitol (BEMAD) method. Eleven phosphorylation sites were localized in the pOBP sequence and nine sites in the VEG sequence. OBPs are secreted by Bowman's gland cells in the extracellular mucus lining the nasal cavity. After tracking the secretion pathway in the rough endoplasmic reticulum of these cells, we hypothesize that these proteins may be phosphorylated by ectokinases that remain to be characterized. The existence of such a regulatory mechanism theoretically increases the number of OBP variants, and it suggests a more specific role for OBPs in odorant coding than the one of odorant solubilizer and transporter.
authors: INRA, UMR 8576 CNRS/Université Lille1, Unité de Glycobiologie Structurale et Fonctionnelle, F-59655, Villeneuve d'Ascq, Cedex, France. patricia.le-meillour@univ-lille1.fr
authors: Patricia
authors: P
authors: Nagnan-Le Meillour
authors: Chrystelle
authors: C
authors: Le Danvic
authors: Fanny
authors: F
authors: Brimau
authors: Philippe
authors: P
authors: Chemineau
authors: Jean-Claude
authors: JC
authors: Michalski
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D054835:Lipocalin 1 / Q000737:chemistry* / Q000378:metabolism; D009831:Olfactory Mucosa / Q000737:chemistry; D010449:Peptide Mapping; D010766:Phosphorylation; D018035:Receptors, Odorant / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa
chemical_list: D054835:Lipocalin 1; D018035:Receptors, Odorant; C045648:odorant-binding protein