New FTIR methodology for the evaluation of (13)C/(12)C isotope ratio in Helicobacter pylori infection diagnosis.
OBJECTIVE
The objective of this work was to assess the reliability of a newly developed FTIR (Fourier Transform Infrared spectroscopy) technique for (13)C-urea breath test in the non-invasive diagnosis of Helicobacter pylori infection, in comparison to those currently used, isotope ratio mass spectrometry (IRMS) and non-dispersive isotope-selective infrared spectrometry (NDIRS). This new methodology is based on the use of a very less expensive and sophisticated FTIR than IRMS and NDIRS.


METHODS
Sixty patients (male and female, aged between 15-70 years) with dyspeptic symptoms, like the epigastric pain, were tested for H. pylori infection using (13)C-urea breath test. Triplicate breath samples were collected before and 30 min after drinking the test solution (75 mg (13)C-urea dissolved in 200 ml 0.1M citric acid). Analysis of delta(13)C were conducted in parallel by means of IRMS, NDIRS and FTIR in order to compare the results.


RESULTS
Delta-over-baseline values were over 5 per thousand in 18 patients, ranging from 8.81 to 60.88 per thousand for IRMS measurements, from 7.2 to 61.2 per thousand for NDIRS and from 7.7 to 61.2 per thousand for FTIR measurements. The remaining 42 subjects had values well below the 5 per thousand cut-off. The results of the baseline-corrected (13)CO(2) exhalation values between FTIR and IRMS/NDIRS were in good agreement.


CONCLUSIONS
The newly developed FTIR methodology has been demonstrated to be a reliable and accurate analytical tool, low cost and easy-to-operate, which permits a highly specific measurement of (13)C enrichment in breath samples. As IRMS and NDIRS it allows to adequately discriminate between infected and non-infected subjects.
Motta
Oriana
O
Department of Educational Science, University of Salerno, 84084 Fisciano (SA), Italy.
De Caro
Francesco
F
Quarto
Floriana
F
Proto
Antonio
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D001944:Breath Tests / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes / Q000032:analysis*; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000191:economics / Q000379:methods*; D014508:Urea / Q000378:metabolism; D055815:Young Adult
D002247:Carbon Isotopes; D002245:Carbon Dioxide; D014508:Urea