Sensitive quantification of bulleyaconitine A in human serum by liquid chromatography-tandem mass spectrometry.
A simple and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of bulleyaconitine A (BLA) in human serum. Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Chromatography separation was performed on a Waters Sunfire C18 column (2.1 x 50 mm, 5 microm) with a gradient mobile phase. Detection was performed in positive mode using multiple reaction monitoring (MRM) of the transition m/z 644.2>584.3 for BLA and m/z 426.3>175.3 for I.S. The proposed method was validated in a linear range of 0.0587-11.7 ng/ml. Intra- and inter-day precision was better than 9.88% and 7.76%. The recovery for BLA was 97.90%, and for I.S. 89.40%. This method provides a simple, rapid, specific, and sensitive tool for the quantitative determination of BLA in human serum, and can be used to monitor serum levels in patients treated with BLA.
Xiong
Xin
X
Department of Pharmacy, Peking University Third Hospital, Beijing, PR China.
Zhai
Suodi
S
Yao
Zhongqiang
Z
D000157:Aconitine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000627:therapeutic use; D000700:Analgesics / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000627:therapeutic use; D001774:Blood Chemical Analysis / Q000379:methods*; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D006801:Humans; D016014:Linear Models; D010003:Osteoarthritis / Q000097:blood / Q000188:drug therapy; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D013997:Time Factors
D000700:Analgesics; D011506:Proteins; C052509:bulleyaconitine A; D000157:Aconitine