Development and validation of LC-MS/MS method for the determination of cyproheptadine in several pharmaceutical syrup formulations.
A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29 min. The analytical method was successfully validated for linearity (1-100 ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98 ng/ml, respectively. The proposed method was applied to analyse the cyproheptadine content from seven different syrup formulations.
Feás
Xesús
X
Department of Analytical Chemistry, Nutrition and Bromatology, Veterinary Faculty, University of Santiago de Compostela, 27002 Lugo, Galiza, Spain. xesusfeas@gmail.com
Ye
Lei
L
Hosseini
Seyed V
SV
Fente
Cristina A
CA
Cepeda
Alberto
A
D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003533:Cyproheptadine / Q000032:analysis*; D004355:Drug Stability; D005506:Food Contamination; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D004364:Pharmaceutical Preparations; D012997:Solvents; D003533:Cyproheptadine