H(2)S and HS(-) donor NaHS inhibits intracellular chloride channels.
We have characterized the effect of H(2)S on single channel properties of the chloride channels derived from the rat heart lysosomal vesicles incorporated into a bilayer lipid membrane. The single chloride channel currents were measured in 250 : 50 mmol/l KCl cis/trans solutions. H(2)S inhibited the chloride channels by decreasing the channel open probability in a concentration-dependent manner. The inhibitory effect of H(2)S was side-dependent with the IC(50) values of 42 and 75 micromol/l for the trans and the cis sides, respectively. The mixture of H2S with the NO donor S-nitroso-N-acetyl-DL-penicillamine had smaller effect (IC(50) = 180 micromol/l) than H(2)S alone. We assume that the inhibitory effect of H(2)S on chloride channels may be responsible for some of its numerous biological effects.
Malekova
Lubica
L
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlárska 5, 833 34 Bratislava, Slovakia.
Krizanova
Olga
O
Ondrias
Karol
K
D000818:Animals; D018118:Chloride Channels / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D006862:Hydrogen Sulfide / Q000737:chemistry / Q000494:pharmacology*; D042541:Intracellular Space / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008564:Membrane Potentials; D008929:Mitochondria, Heart / Q000737:chemistry; D020030:Nitric Oxide Donors / Q000737:chemistry / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011336:Probability; D051381:Rats; D026423:S-Nitroso-N-Acetylpenicillamine / Q000737:chemistry / Q000494:pharmacology; D013440:Sulfides / Q000737:chemistry / Q000494:pharmacology*
D018118:Chloride Channels; D008051:Lipid Bilayers; D020030:Nitric Oxide Donors; D013440:Sulfides; D026423:S-Nitroso-N-Acetylpenicillamine; C025451:sodium bisulfide; D006862:Hydrogen Sulfide