Iontophoretic delivery of lisinopril: Optimization of process variables by Box-Behnken statistical design.
The objective of the investigation was to optimize the iontophoresis process parameters of lisinopril (LSP) by 3 x 3 factorial design, Box-Behnken statistical design. LSP is an ideal candidate for iontophoretic delivery to avoid the incomplete absorption problem associated after its oral administration. Independent variables selected were current (X(1)), salt (sodium chloride) concentration (X(2)) and medium/pH (X(3)). The dependent variables studied were amount of LSP permeated in 4 h (Y(1): Q(4)), 24 h (Y(2): Q(24)) and lag time (Y(3)). Mathematical equations and response surface plots were used to relate the dependent and independent variables. The regression equation generated for the iontophoretic permeation was Y(1) = 1.98 + 1.23X(1) - 0.49X(2) + 0.025X(3) - 0.49X(1)X(2) + 0.040X(1)X(3) - 0.010X(2)X(3) + 0.58X(1)(2) - 0.17X(2)(2) - 0.18X(3)(2); Y(2) = 7.28 + 3.32X(1) - 1.52X(2) + 0.22X(3) - 1.30X(1)X(2) + 0.49X(1)X(3) - 0.090X(2)X(3) + 0.79X(1)(2) - 0.62X(2)(2) - 0.33X(3)(2) and Y(3) = 0.60 + 0.0038X(1) + 0.12X(2) - 0.011X(3) + 0.005X(1)X(2) - 0.018X(1)X(3) - 0.015X(2)X(3) - 0.00075X(1)(2) + 0.017X(2)(2) - 0.11X(3)(2). The statistical validity of the polynomials was established and optimized process parameters were selected by feasibility and grid search. Validation of the optimization study with 8 confirmatory runs indicated high degree of prognostic ability of response surface methodology. The use of Box-Behnken design approach helped in identifying the critical process parameters in the iontophoretic delivery of lisinopril.
Gannu
Ramesh
R
National Facilities in Engineering and Technology with Industrial Collaboration (NAFETIC), University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India.
Yamsani
Vamshi Vishnu
VV
Palem
Chinna Reddy
CR
Yamsani
Shravan Kumar
SK
Yamsani
Madhusudan Rao
MR
D000279:Administration, Cutaneous; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D006863:Hydrogen-Ion Concentration; D007478:Iontophoresis; D017706:Lisinopril / Q000008:administration & dosage / Q000493:pharmacokinetics*; D015233:Models, Statistical; D010539:Permeability; D051381:Rats; D012044:Regression Analysis; D012869:Skin Absorption; D012965:Sodium Chloride / Q000737:chemistry; D013997:Time Factors
D000806:Angiotensin-Converting Enzyme Inhibitors; D012965:Sodium Chloride; D017706:Lisinopril