Optimization of axial resolution in a confocal microscope with D-shaped apertures.
We show theoretically that the axial resolution is improved when two centrosymmetric D-shaped apertures are combined in a confocal microscope with a finite-sized pinhole. The optimum width of a divider that separates the D-shaped apertures to give the maximum axial resolution for a given pinhole size is investigated, and the magnitude of the signal level is explored.
Gong
Wei
W
Division of Bioengineering, National University of Singapore, Singapore 117576.
Si
Ke
K
Sheppard
Colin J R
CJ
D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D055096:Optical Devices