Ligation or cross-linking of CD40 has different effects on AGS gastric cancer cells.
A gastric cancer (GC) cell line, AGS, has high-level expression of CD40, a tumor necrosis factor receptor (TNFR) family member. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating diverse cellular responses, such as proliferation, differentiation, growth suppression, and cell death. In this research, we studied the effects of different forms of CD40 stimulation on AGS cells by flow cytometry, Western blotting and siRNA transfection. We found that different forms of CD40 stimulation, either recombinant soluble CD40L (sCD40L, ligation) or agonist anti-CD40 antibody (cross-linking), induced different effects in AGS gastric cancer cells, proliferation or apoptosis. We also showed that VEGF provided a significant contribution to sCD40L-induced proliferation, while agonist anti-CD40 antibody induced GADD45 upregulation and promoted apoptosis.
Qi
Chun-Jian
CJ
Department of Oncology, the Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou, PR China.
Qian
Ke-Qing
KQ
Ning
Yong-Ling
YL
Ma
Hong-Bing
HB
Wang
Shi-Zhong
SZ
Zhang
Xue-Guang
XG
D017209:Apoptosis / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology* / Q000494:pharmacology; D023201:CD40 Ligand / Q000276:immunology* / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002867:Chromones / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D009025:Morpholines / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics / Q000276:immunology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000276:immunology*; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology; D000097642:GADD45 Proteins
D019013:CD40 Antigens; D023201:CD40 Ligand; D018797:Cell Cycle Proteins; D002867:Chromones; D007211:Indoles; D009025:Morpholines; D009687:Nuclear Proteins; D047428:Protein Kinase Inhibitors; D011758:Pyrroles; D012313:RNA; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; C439070:GADD45A protein, human; D000097642:GADD45 Proteins; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C116890:Semaxinib