Glycosaminoglycan degradation-inhibitory lactic acid bacteria ameliorate 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice.
To evaluate the effects of lactic acid bacteria (LAB) in inflammatory bowel diseases (IBD), we measured the inhibitory effect of several LAB isolated from intestinal microflora and commercial probiotics against the glycosaminoglycan (GAG) degradation by intestinal bacteria. Bifidobacterium longum HY8004 and Lactobacillus plantarum AK8-4 exhibited the most potent inhibition. These LAB inhibited colon shortening and myeloperoxidase production in 2,4,6- trinitrobenzenesulfonic acid (TNBS)-induced experimental colitic mice. These LAB also blocked the expression of the proinflammatory cytokines, IL-1beta and TNF-alpha, as well as of COX-2, in the colon. LAB also blocked activation of the transcription factor, NF-kappaB, and expression of TLR-4 induced by TNBS. In addition, LAB reduced the TNBS-induced bacterial degradation activities of chondroitin sulfate and hyaluronic acid. These findings suggest that GAG degradation-inhibitory LAB may improve colitis by inhibiting inflammatory cytokine expression via TLR-4-linked NF-kB activation and by inhibiting intestinal bacterial GAG degradation.
Lee
Bomi
B
Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Korea.
Lee
Jung-Hee
JH
Lee
Hye-Sung
HS
Bae
Eun-Ah
EA
Huh
Chul-Sung
CS
Ahn
Young-Tae
YT
Kim
Dong-Hyun
DH
D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000382:microbiology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D019936:Probiotics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D006025:Glycosaminoglycans; D053583:Interleukin-1beta; D016328:NF-kappa B; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D014302:Trinitrobenzenesulfonic Acid; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2