title: The prevalence of virulence genes of E. coli strains isolated from children with urinary tract infection.
abstract: To evaluate the prevalence of virulence genes in E. coli strains isolated from urine samples of children with urinary tract infection(UTI) and their correlation with clinical data, we isolated E. coli strains from urine samples of children with UTI during the period of August 2005 - August 2006 and studied them for the presence of the virulence genes by PCR. A total of 96 E. coli strains were isolated. The prevalence of genes, pyelonephritis associated pili (pap genes), S-family adhesions (sfa gene), hemolysin (hly gene), and cytotoxic nercotizing factor type 1 (cnf-1-1 gene) among the isolated strains was 27.1%, 14.6%, 13.5% and 22.9 %, respectively. Pyelonephritis was more prevalent in the cases with positive virulence genes. The results showed significant correlation between age of the patient and the presence of the genes (P< 0.05). Cnf-1 gene was significantly more common in samples of patients with abnormal finding on the ultrasound of kidneys (P= 0.049). Our study demonstrated higher prevalence of pyelonephritis in the presence of E. coli virulence genes. Detection of the genes in urine samples may help in the management of UTI.
authors: Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: Shohreh
authors: S
authors: Farshad
authors: Fatemeh
authors: F
authors: Emamghorashi
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics / Q000302:isolation & purification; D010861:Fimbriae, Bacterial / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction; D011704:Pyelonephritis / Q000209:etiology / Q000382:microbiology; D014552:Urinary Tract Infections / Q000150:complications / Q000382:microbiology*; D037521:Virulence Factors / Q000302:isolation & purification*
chemical_list: D018830:Adhesins, Escherichia coli; D004269:DNA, Bacterial; C092426:PapG protein, E coli; D037521:Virulence Factors; D036781:Fimbriae Proteins