Isolation and characterization of rice straw degrading Streptomyces griseorubens C-5.
To reutilize rice straw generated during the agricultural production process, the actinomycete strain C-5 was isolated from soil that was under the stook for several years in the Heilongjiang province of China by using multiple selective culture media. Strain C-5 was identified as Streptomyces griseorubens by China General Microbiological Culture Collection Center (CGMCC) through morphological and physiological characterization combined with the result of 16S rRNA gene sequence and data analysis. This strain has simultaneous cellulase, laccase, peroxidase, xylanase and pectinase activity. The various chemical composition of rice straw were determined during fermentation process. Simultaneously the biodegradation process of rice straw stem was observed by scanning electron microscope (SEM). It is predicted that strain C-5 could decompose rice straw effectively and had promising prospects of being applied in improving the resource utilization of rice straw.
Xu
Jie
J
Department of Life Science and Engineering, Harbin Institute of Technology, 150001 Harbin, China. xj3455@sina.com.cn
Yang
Qian
Q
D001426:Bacterial Proteins / Q000378:metabolism; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005285:Fermentation; D012275:Oryza / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S