title: Exposure to high static or pulsed magnetic fields does not affect cellular processes in the yeast Saccharomyces cerevisiae.
abstract: We report results of a study of the effects of strong static (up to 16 T for 8 h) and pulsed (up to 55 T single-shot and 4 x 20 T repeated shots) magnetic fields on Saccharomyces cerevisiae cultures in the exponential phase of growth. In contrast to previous reports restricted to only a limited number of cellular parameters, we have examined a wide variety of cellular processes: genome-scale gene expression, proteome profile, cell viability, morphology, and growth, metabolic and fermentation activity after magnetic field exposure. None of these cellular activities were impaired in response to static or pulsed magnetic field exposure. Our results confirm and extend previous reports on the absence of magnetic field effects on yeast and support the hypothesis that magnetic fields have no impact on the transcriptional machinery and on the integrity of unicellular biological systems.
authors: University of Toulouse, INSA, UPS, INP & INRA, Toulouse, France. leberre@insa-toulouse.fr
authors: Veronique
authors: V
authors: Anton-Leberre
authors: Evert
authors: E
authors: Haanappel
authors: Nathalie
authors: N
authors: Marsaud
authors: Lidwine
authors: L
authors: Trouilh
authors: Laurent
authors: L
authors: Benbadis
authors: Helian
authors: H
authors: Boucherie
authors: Sophie
authors: S
authors: Massou
authors: Jean M
authors: JM
authors: François
mesh_terms: D002468:Cell Physiological Phenomena / Q000502:physiology*; D004574:Electromagnetic Fields; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D005656:Fungal Proteins / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D020543:Proteome / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D013997:Time Factors
chemical_list: D005656:Fungal Proteins; D020543:Proteome; D012333:RNA, Messenger; D000431:Ethanol; D005947:Glucose; D005990:Glycerol