title: Confirmation of diabetes-related quantitative trait loci derived from SM/J and A/J mice by using congenic strains fed a high-carbohydrate or high-fat diet.
abstract: The interaction between causative genes and diet is known to influence the onset of obesity and diabetes in humans, although it has remained difficult to identify diabetogenic gene(s) because humans are genetically and environmentally heterogeneous. Mouse SMXA recombinant inbred (RI) strains are established from parental inbred strains (SM/J and A/J) and have been shown to be beneficial tools for analyzing polygenic traits. We previously mapped a significant quantitative trait locus (QTL, T2dm1sa) on Chromosome (Chr.) 10 and suggestive QTLs on Chr. 2, 6, and 18 for diabetes-related traits by using SMXA RI strains fed a high-carbohydrate diet. As a first step in identifying the responsible gene among QTLs for glucose tolerance mapped on Chr. 10 and 18, we established new strains of A.SM-T2dm1sa and SM.A-D18Mit19-D18Mit7 congenic mice. Each congenic strain bears the diabetogenic allele of an introgressed chromosomal region on a genetic background strain carrying the non-diabetogenic allele. The diabetogenic effect of T2dm1sa mapped on Chr. 10 was not supported by studies of A.SM-T2dm1sa congenic mice when the mice were fed a high-carbohydrate or high-fat diet. SM.A-D18Mit19-D18Mit7 congenic mice showed impaired glucose tolerance not only when they were fed a high-carbohydrate diet, but also when they were fed a high-fat diet. Thus, it appears that gene(s) affecting diabetes-related traits under either dietary condition may be present on Chr. 18.
authors: Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
authors: Misato
authors: M
authors: Kobayashi
authors: Natsuko
authors: N
authors: Hada
authors: Hiromi
authors: H
authors: Hoshino
authors: Takashi
authors: T
authors: Ozawa
authors: Kazuhiko
authors: K
authors: Umeshita
authors: Masahiko
authors: M
authors: Nishimura
authors: Atsushi
authors: A
authors: Murai
authors: Tamio
authors: T
authors: Ohno
authors: Fumihiko
authors: F
authors: Horio
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001823:Body Composition; D001835:Body Weight; D002874:Chromosome Mapping; D003920:Diabetes Mellitus / Q000235:genetics*; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D051379:Mice; D020297:Mice, Congenic; D008805:Mice, Inbred A; D018895:Microsatellite Repeats / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*
chemical_list: D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D007328:Insulin