Differential expression of NUDT9 at different phases of the menstrual cycle and in different components of normal and neoplastic human endometrium.
OBJECTIVE
The human endometrium manifests different and distinct morphologies and physiologies during the different phases of the menstrual cycle. We aimed to determine which candidate genes demonstrate differential expression patterns in the endometrium during different phases of the menstrual cycle.


METHODS
Using differential display reverse transcription polymerase chain reaction to compare day 5 and day 18 human glandular endometrium obtained by laser capture microdissection, we identified a specific gene, NUDT9 (nucleoside diphosphate-linked moiety X motif 9). NUDT9 is known to function as a highly specific adenosine diphosphate ribose pyrophosphatase and has been mapped to chromosome 4q22.1. It gives rise to two alternatively spliced messenger RNAs, NUDT9alpha and NUDT9beta, encoding a member of the Nudix hydrolase family. In this study, we purified NUDT9 protein and produced an antibody, which we then used for immunohistochemical studies.


RESULTS
Using this anti-NUDT9 antibody, we successfully demonstrated that NUDT9 protein was differentially expressed in endometrial glandular cells at different phases of the menstrual cycle. NUDT9 was also found to be expressed more prominently in the epithelial glandular component than in the stromal component of human endometrial carcinomas.


CONCLUSIONS
We suggest that NUDT9 may be involved in the regulation of the menstrual cycle and may be related to the proliferation of glandular cells in the human endometrium.
Chen
Ling-Yun
LY
Institute of Biochemistry and Biotechnology, School of Medicine, Chung-Shan Medical University, Taichung, Taiwan.
Chen
Tze-Ho
TH
Wen
Pao-Ying
PY
Chou
Chia-Hung
CH
Ying
Tsung-Ho
TH
Chang
Shun-Ping
SP
Ma
Gwo-Chin
GC
Chen
Ming
M
D000328:Adult; D000595:Amino Acid Sequence; D000906:Antibodies / Q000494:pharmacology; D002894:Chromosomes, Human, Pair 4; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D004717:Endometrium / Q000473:pathology / Q000502:physiology*; D005260:Female; D005498:Follicular Phase / Q000235:genetics; D006801:Humans; D008183:Luteal Phase / Q000235:genetics; D008597:Menstrual Cycle / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D010060:Ovulation / Q000235:genetics; D011755:Pyrophosphatases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000096964:Nudix Hydrolases
D000906:Antibodies; D011755:Pyrophosphatases; D012333:RNA, Messenger; C469383:NUDT10 protein, human; D000096964:Nudix Hydrolases