Differential expressed protein in developing stages of Nepenthes gracilis Korth. pitcher.
Nepenthes gracilis Korth. is a member of carnivorous plants in family Nepenthaceae. The plants have beautiful and economically important pitchers. It is interesting to study the protein(s) correlated with the pitcher. Crude proteins were extracted from leaf, leaf with developing pitcher and developed pitcher of the same plant and analyzed by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Two protein bands with molecular weights of 42.7 and 38 kDa were obtained from young leaf and leaf with developing pitcher, respectively. The 42.7 kDa protein was identified as phosphoglycerate kinase (PGK) by Liquid Chromatography Mass Spectrometry (LC-MS/MS), but the 38 kDa band is an unknown protein. Both proteins were differentially expressed in each developing stage of the pitcher, thus may be powerful candidates play role in development pathway of leaf and pitcher.
Pinthong
Krit
K
Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Surin 32000, Thailand.
Chaveerach
Arunrat
A
Tanee
Tawatchai
T
Sudmoon
Runglawan
R
Mokkamul
Piya
P
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D052583:Sarraceniaceae / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment
D010936:Plant Extracts; D010940:Plant Proteins; D010735:Phosphoglycerate Kinase