title: Forkhead transcription factor Foxo1 is essential  for adipocyte differentiation.
abstract: We analyzed the physiological role of forkhead box class O 1 (Foxo1) in adipocyte differentiation by suppressing Foxo1 mRNAwith siRNA specific for Foxo1.Mouse 3T3-L1 preadipocytes infected with an adenovirus expressing Foxo1-siRNA showed a marked decrease in lipid droplet formation when induced to differentiate into adipocytes. Adipocyte differentiation was most severely inhibited by exposing cells to Foxo1-siRNA before induction of differentiation. The incorporation of fluorescent-labeled glucose and fatty acid was significantly inhibited in cells deficient in Foxo1. RTPCR revealed that downregulation of Foxo1 decreased the expression of the transcription factors, PPAR-γ and C/EBP-α. By comparison, Foxo1-siRNA did not affect the expression of C/EBP-β or C/EBP-δ during the early period of adipocyte differentiation. These results indicate that Foxo1 plays an essential role in adipocyte differentiation, especially at the very early stage of terminal adipocyte differentiation.
authors: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, Japan.
authors: Keisuke
authors: K
authors: Munekata
authors: Kazuichi
authors: K
authors: Sakamoto
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D019556:COS Cells; D002454:Cell Differentiation / Q000235:genetics*; D002522:Chlorocebus aethiops; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000037:antagonists & inhibitors / Q000502:physiology*; D050356:Lipid Metabolism / Q000235:genetics; D051379:Mice; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; D012333:RNA, Messenger