title: Rapid identification of lactic acid bacteria isolated from home-made fermented milk in Tibet.
abstract: Fermented milk is a very delicious and nutritional food distributed in the international markets. Rapid preliminary identification of lactic acid bacteria to the species level is an important issue for the fermentation industry. One hundred seventy-one strains of lactic acid bacteria (LAB) were isolated from twenty-nine kurut samples and fifteen traditional fermented mongolian cattle milk (FMCM) samples in Tibet, China. All of the strains were isolated and divided into bacilli or cocci by phenotype, and then differentiated by restriction fragment length polymorphism (RFLP) analysis using a set of restriction enzymes, AluI, HaeIII, BsmaI, TspRI and HinfI. Restriction pattern analyses indicated that the lactobacilli could be clearly identified at the species level and the LAB cocci at the genera level by the five restriction enzymes. Further studies on 16S rDNA sequences of representative and undefined strains showed that the combination of RFLP analysis of polymerase chain reaction (PCR) for amplifying 16S rDNA and 16S rDNA sequence analysis is rapid, easy to perform, and effective for large-scale preliminary identification of LAB.
authors: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, PRC.
authors: Jie
authors: J
authors: Yu
authors: Zhihong
authors: Z
authors: Sun
authors: Wenjun
authors: W
authors: Liu
authors: Jiachao
authors: J
authors: Zhang
authors: Tiansong
authors: T
authors: Sun
authors: Qiuhua
authors: Q
authors: Bao
authors: Heping
authors: H
authors: Zhang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D043302:Cultured Milk Products / Q000382:microbiology*; D005516:Food Microbiology; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D018609:Tibet
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S