Stress responses to heterologous membrane protein expression in Escherichia coli.
The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms.
Xu
Lucy Y
LY
Department of Chemical Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544, USA.
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A James
AJ
D049069:ATP-Dependent Proteases / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D006801:Humans; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012808:Sigma Factor / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins; D043884:Receptor, Cannabinoid, CB1; D011994:Recombinant Proteins; D012808:Sigma Factor; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli