title: Human adipose-derived stem cells contribute to chondrogenesis in coculture with human articular chondrocytes.
abstract: Adipose tissue is easily available and contains high numbers of stem cells that are capable for chondrogenic differentiation. We hypothesize that a partial substitution of chondrocytes with autologous adipose-derived stem cells (ASC) might be a possible strategy to reduce the number of chondrocytes needed in matrix-associated autologous chondrocyte transplantation. To lay the ground, in vitro coculture experiments were performed using human chondrocytes and human ASC. Chondrocytes were obtained from donors undergoing matrix-associated autologous chondrocyte transplantation. ASC were isolated from liposuction material. Chondrocytes and ASC were seeded either in fibrin (Tisseel; Baxter, Vienna, Austria) or collagen matrix (Tissue Fleece; Baxter, Unterschleissheim, Germany). RNA for quantitative reverse transcriptase (RT)-polymerase chain reaction was isolated after 2 weeks of culture in chondrogenic medium, and after 4 weeks samples were processed for histology. Related to the number of chondrocytes used, coculture with ASC led to strong increase in collagen type IX mRNA expression, which is an indicator for long-term stability of cartilage. Moderate upregulation was shown for SOX9, aggrecan, melanoma inhibitory activity, cartilage link protein 1, and cartilage oligomeric matrix protein mRNA. However, expression of collagen I and collagen II indicates the synthesis of fibrous tissue, which might be due to the use of dedifferentiated chondrocytes. Tisseel provided slightly better chondrogenic conditions than Tissue Fleece. These data support the possibility to take advantage of ASC in cartilage regeneration in conjunction with autologous chondrocytes.
authors: AUVA Research Center, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.
authors: Florian
authors: F
authors: Hildner
authors: Sebastian
authors: S
authors: Concaro
authors: Anja
authors: A
authors: Peterbauer
authors: Susanne
authors: S
authors: Wolbank
authors: Martin
authors: M
authors: Danzer
authors: Anders
authors: A
authors: Lindahl
authors: Paul
authors: P
authors: Gatenholm
authors: Heinz
authors: H
authors: Redl
authors: Martijn
authors: M
authors: van Griensven
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D002358:Cartilage, Articular / Q000166:cytology*; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis; D018920:Coculture Techniques / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism