Differential expression of p63 isoforms in normal skin and hyperproliferative conditions.
BACKGROUND
The p63 is regarded as a potential stem cell marker.


METHODS
Expression of p63 isoforms was examined in normal skin and hyperproliferative conditions including psoriasis and artificial skin equivalents (SEs). Rapidly adhering (RA) and slowly adhering (SA) cells were isolated, and Western blotting was performed.


RESULTS
Expression of p63 (4A4) and p63 (H-137) is similar in all conditions, although there is some variation in psoriasis. However, expression of p63alpha (C-12) is markedly different. In normal skin, p63alpha (C-12)-positive cells were scattered in whole epidermis. But in psoriasis, p63alpha (C-12)-positive cells were observed at the tips of rete ridges. In SEs, p63alpha (C-12)-positive cells were not well observed. Western blot results showed that the RA cells express p63 (4A4) and p63 (H-137) strongly compared with SA or nonadhering (NA) cells. In contrast, SA or NA cells strongly express p63alpha (C-12).


CONCLUSIONS
Results suggest that both p63 (4A4) and p63 (H-137) can detect epidermal stem cells. But, p63 (H-137) seemed to be a better marker because p63 (H-137)-positive cells were more localized at basal layer. In addition, it can be said that p63alpha (C-12) can detect TAp63, which is important in differentiation of epidermis. Furthermore, it is concluded that molecular control of TAp63 is especially disorganized in hyperproliferative condition including psoriasis and SEs.
Kim
So-Young
SY
Department of Biochemistry, College of Medicine, Chung-Ang University, Heukseok-Dong, Dongjak-Gu, Seoul, Korea.
Cho
Hyun-Joo
HJ
Kim
Dong-Seok
DS
Choi
Hae-Ryung
HR
Kwon
Sun-Bang
SB
Na
Jung-Im
JI
Jeon
Hye-Chan
HC
Huh
Chang-Hoon
CH
Youn
Sang-Woong
SW
Cho
Kwang-Hyun
KH
Park
Kyoung-Chan
KC
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D020033:Protein Isoforms / Q000096:biosynthesis; D011565:Psoriasis / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D015534:Trans-Activators / Q000096:biosynthesis*; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*
D015415:Biomarkers; D020033:Protein Isoforms; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins