Ferritin heavy chain (FHC) is up-regulated in papillomavirus-associated urothelial tumours of the urinary bladder in cattle.
The up-regulation of ferritin heavy chain (FHC) is reported in six papillary and in four invasive urothelial tumours of the urinary bladder of cattle grazing on mountain pastures rich in bracken fern. All tumours contained sequence of bovine papillomavirus type-2 (BPV-2) as determined by polymerase chain reaction (PCR) analyses and validated by direct sequencing of the amplified products. The oncoprotein E5 was also detected in these tumours by immunoprecipitation and by immunofluorescence and laser scanning confocal microscopy. Expression of FHC was evaluated by western blot analysis, reverse transcriptase (RT) PCR, real-time RT-PCR and immunohistochemistry. The oligonucleotide sequence of the bovine ferritin amplicons was identical to that of human ferritin. Nuclear overexpression of p65, an important component of nuclear factor kappaB (NF-kappaB) transcription factors, was also observed. These findings suggest that FHC up-regulation may be mediated by activation of NF-kappaB and that in turn this may be related to the resistance of bovine papillomavirus type-2 (BPV-2) infected urothelial cells to apoptosis.
Roperto
S
S
Department of Veterinary Pathology and Animal Health, Naples University Federico II, Naples, Italy. sante.roperto@unina.it
Borzacchiello
G
G
Brun
R
R
Costanzo
F
F
Faniello
M C
MC
Raso
C
C
Rosati
A
A
Russo
V
V
Leonardi
L
L
Saracino
D
D
Turco
M C
MC
Urraro
C
C
Roperto
F
F
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000821:virology; D024202:Electrophoretic Mobility Shift Assay; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000096:biosynthesis; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism* / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000662:veterinary* / Q000821:virology
D016328:NF-kappa B; D005293:Ferritins