[Study on single nucleotide polymorphisms of H-FABP gene in sheep].
PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan x SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this study. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G ) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan x SH F1 populations (PIC amp; 0.25) but an intermediate PIC in the remaining three populations (0.25 amp; PIC amp;0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HHHhhh. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC amp; 0.25). However, there was no polymorphisms in SH, Tan x SH F1 and Suffolk populations.
Qiao
Hai-Yun
HY
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China. qiaohaiyun711@163.com
Zhao
Qian-Jun
QJ
Yao
Na
N
Lu
Ya-Zhou
YZ
Meng
Xiang-Ren
XR
Han
Jian-Lin
JL
Guan
Wei-Jun
WJ
Ma
Yue-Hui
YH
D000818:Animals; D001483:Base Sequence; D001947:Breeding; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency; D007438:Introns / Q000235:genetics; D008460:Meat; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D012756:Sheep / Q000235:genetics*
D050556:Fatty Acid-Binding Proteins