X-cells in pseudotumors of yellowfin goby Acanthogobius flavimanus: a protistan organism distinct from that in flathead flounder Hippoglossoides dubius.
Yellowfin goby Acanthogobius flavimanus affected with X-cell pseudotumors were sampled from a river estuary in Tokyo Bay, Japan. We amplified the gene for small subunit ribosomal RNA (18S rRNA) of X-cells of the goby with PCR using universal primers. The gene that we obtained (DDBJ Accession no. AB451874) showed 91% sequence identity to that of the X-cells of the flathead flounder Hippoglossoides dubius. With in situ hybridization, the probes specific for the gene that we obtained hybridized with the goby X-cells but not with the flounder X-cells, whereas probes for the 18S rRNA gene of flounder X-cells hybridized with the flounder X-cells but not with goby X-cells. These findings indicate that, although the X-cells found in the goby are closely related to the protist found in flounder, the two are clearly distinct organisms.
Miwa
Satoshi
S
Inland Station, National Research Institute of Aquaculture, Tamaki, Mie 519-0423, Japan. miwasat@affrc.go.jp
Kamaishi
Takashi
T
D000818:Animals; D056890:Eukaryota / Q000145:classification* / Q000235:genetics*; D005393:Fish Diseases / Q000469:parasitology; D005432:Flounder / Q000469:parasitology*; D006104:Granuloma, Plasma Cell / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D007564:Japan; D008969:Molecular Sequence Data; D010473:Perciformes / Q000469:parasitology*; D010802:Phylogeny; D011529:Protozoan Infections, Animal / Q000469:parasitology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D012337:RNA, Ribosomal, 18S