title: Rapid and cost-effective SNP detection method: application of SmartAmp2 to pharmacogenomics research.
abstract: Pharmacogenomics data can facilitate our understanding of the sources of variability in drug response, which can potentially lead to improved safety and efficacy of drug therapy for individual patients. A key requirement for the development of individualized medicine or personalized therapy is the ability to rapidly and conveniently test patients for genetic polymorphisms and/or mutations. However, in today's world, genotyping technology remains a bottleneck in clinical applications because of its slow speed and high cost. Therefore, we have recently developed a rapid and cost-effective method for SNP detection, named Smart Amplification Process 2 (SmartAmp2), which enables us to detect genetic polymorphisms or mutations in 30-45 min under isothermal conditions without DNA isolation and PCR amplification. This article presents the SNP detection method and its underlying molecular mechanism as well as clinical research applications.
authors: KK DNAFORM, Kanagawa, Japan.
authors: Yasumasa
authors: Y
authors: Mitani
authors: Alexander
authors: A
authors: Lezhava
authors: Aki
authors: A
authors: Sakurai
authors: Akina
authors: A
authors: Horikawa
authors: Makoto
authors: M
authors: Nagakura
authors: Yoshihide
authors: Y
authors: Hayashizaki
authors: Toshihisa
authors: T
authors: Ishikawa
mesh_terms: D003362:Cost-Benefit Analysis / Q000191:economics / Q000379:methods; D006801:Humans; D010597:Pharmacogenetics / Q000191:economics* / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013997:Time Factors