Compact quantum dot probes for rapid and sensitive DNA detection using highly efficient fluorescence resonant energy transfer.
We developed a simple method for quickly synthesizing compact quantum dot (QD)-DNA probes for sensitive DNA detection using fluorescence resonant energy transfer (FRET). The density of DNA probes on the QD surface was controlled to avoid steric hindrance and to promote rapid hybridization with target DNA molecules. The radius of the final QDs was only around 3 nm after applying the functional coating, enabling highly efficient energy transfer. It was demonstrated that nearly 70% transfer efficiency could be achieved with only a few DNA molecules on each QD and that the FRET-based DNA detection could be carried out within 10 min with a sub-nM detection limit. Theoretical analysis was also performed to confirm our results.
Wu
Chung-Shieh
CS
Biological Engineering Department, University of Missouri, 240D Bond Life Sciences Center, 1201 E Rollins Street, Columbia, MO 65211, USA.
Cupps
Jay M
JM
Fan
Xudong
X
D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D009693:Nucleic Acid Hybridization; D010316:Particle Size; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013997:Time Factors
D015342:DNA Probes; D004247:DNA