title: Effects of endotoxin and catecholamines on hepatic mitochondrial respiration.
abstract: Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.
authors: Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, Bern, Switzerland.
authors: Francesca
authors: F
authors: Porta
authors: Hendrik
authors: H
authors: Bracht
authors: Christian
authors: C
authors: Weikert
authors: Mario
authors: M
authors: Beck
authors: Jukka
authors: J
authors: Takala
authors: Sebastian
authors: S
authors: Brandt
authors: Luzius B
authors: LB
authors: Hiltebrand
authors: Stephan M
authors: SM
authors: Jakob
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology*; D019069:Cell Respiration / Q000502:physiology; D004731:Endotoxins / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D013552:Swine; D013997:Time Factors
chemical_list: D002395:Catecholamines; D004731:Endotoxins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate