[Relationship between IL-10 promoter gene polymorphisms and the susceptibility to endometriosis].
The purpose of this study was to investigate the relationship between IL-10 promoter gene polymorphism and the susceptibility to endometriosis (EMs) in Chinese population. Amplification refractory mutation system polymerase chain reactions (ARMS-PCR) and DNA sequencing were performed to detect polymorphism of -1082G/A site and PCR-RFLP was used to determine the genotypes in -819T/C and -592A/C sites. One hundred and nineteen Chinese women with different stage EMs and 120 controls were employed in this study. Although there were no significant differences in the polymorphism of -1082 site between two groups (P>0.05), the frequencies of -819C, -592C alleles and -819 CC+TC, -592 CC+AC genotypes were significantly increased in EMs patients compared with the controls (P<0.05). The frequencies of -819C, -592C alleles and -819 CC+TC, -592 CC+AC genotypes were significantly increased in III-IV EMs patients compared with I-IIMs or controls (P<0.01). This suggests that polymorphisms of IL-10-819 (T/C) and -592 (A/C) may be associated with the susceptibility to EMs in Chinese population.
He
Pei
P
The Obstetrics and Gynecology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China. heipei2@sina.com
Zhang
Xin-Mei
XM
Deng
Lin
L
Ma
Jun-Yan
JY
D000328:Adult; D044466:Asian People / Q000235:genetics; D004198:Disease Susceptibility / Q000209:etiology*; D004715:Endometriosis / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000502:physiology
D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10