Genotoxicity of sodium arsenite and DNA fragmentation in ovarian cells of rat.
Present study examined the genotoxic effects of arsenite in ovarian tissue of rat at 56 days of age. Immature (28 days old) female rats were exposed to different doses (50, 100, and 200 ppm) of sodium arsenite in drinking water for 28 days. DNA damage in ovarian tissue was measured by comet assay. All doses induced significant decrease in ovarian weight in a dose-dependent manner compared to control, more prominently at (P<0.001) 100 and 200 ppm. All the comet assay parameters showed significant difference with arsenite treatment compared to control group. In treatment groups, mean number of cells with intact DNA decreased while, mean comet number increased (P<0.001) in a dose-dependent manner compared to control. Significant decrease (P<0.05) was observed in mean comet length, height, comet head diameter and %DNA in comet head of high dose groups compared to control group. Dose dependent increase was found in mean comet tail length, %DNA in tail, tail moment and olive tail moment in high dose groups compared to control group. The study indicates that arsenic caused DNA damage to ovarian cells particularly at high doses and ensure comet assay as an effective method to detect DNA damage in tissue caused by metals.
Akram
Zertashia
Z
Faculty of Biological Sciences, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan. zee1524@yahoo.com
Jalali
Samina
S
Shami
Sajjad Aslam
SA
Ahmad
Laiq
L
Batool
Sajida
S
Kalsoom
Ommia
O
D000284:Administration, Oral; D000818:Animals; D018053:Arsenites / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D002452:Cell Count; D020552:Comet Assay; D053938:DNA Fragmentation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008856:Microscopy, Fluorescence; D009153:Mutagens / Q000493:pharmacokinetics / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017670:Sodium Compounds / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*
D018053:Arsenites; D009153:Mutagens; D017670:Sodium Compounds; C017947:sodium arsenite