Identification of neural programmed cell death through the detection of DNA fragmentation in situ and by PCR.
Programmed cell death is a fundamental process for the development and somatic maintenance of organisms. This unit describes methods for visualizing both dying cells in situ and for detection of nucleosomal ladders. A description of various current detection strategies is provided, as well as support protocols for preparing positive and negative controls and for preparing genomic DNA.
Yung
Yun C
YC
Helen L. Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The Scripps Research Institute, La Jolla, California, USA.
Kennedy
Grace
G
Chun
Jerold
J
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053938:DNA Fragmentation; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D020287:In Situ Nick-End Labeling / Q000379:methods*; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods*