Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions.
The "phiKMV-like viruses" comprise an important genus of T7 related phages infecting Pseudomonas aeruginosa. The genomes of these bacteriophages have localized single-strand interruptions (nicks), a distinguishing genomic trait previously thought to be unique for T5 related coliphages. Analysis of this feature in the newly sequenced phage phikF77 shows all four nicks to be localized on the non-coding DNA strand. They are present with high frequencies within the phage population and are introduced into the phage DNA at late stages of the lytic cycle. The general consensus sequence in the nicks (5'-CGACxxxxxCCTAoh pCTCCGG-3') was shown to be common among all phiKMV-related phages.
Kulakov
Leonid A
LA
School of Biological Sciences, The Queen's University of Belfast, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland. l.kulakov@qub.ac.uk
Ksenzenko
Vladimir N
VN
Shlyapnikov
Michael G
MG
Kochetkov
Vladimir V
VV
Del Casale
Antonio
A
Allen
Christopher C R
CC
Larkin
Michael J
MJ
Ceyssens
Pieter-Jan
PJ
Lavigne
Rob
R
D016384:Consensus Sequence; D053904:DNA Breaks, Single-Stranded; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D017902:Podoviridae / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*
D004279:DNA, Viral