Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2.
Porphyromonas gingivalis, a causative agent of periodontitis, has at least two types of thin, single-stranded fimbriae, termed FimA and Mfa1 (according to the names of major subunits), which can be discriminated by filament length and by the size of their major fimbrilin subunits. FimA fimbriae are long filaments that are easily detached from cells, whereas Mfa1 fimbriae are short filaments that are tightly bound to cells. However, a P. gingivalis ATCC 33277-derived mutant deficient in mfa2, a gene downstream of mfa1, produced long filaments (10 times longer than those of the parent), easily detached from the cell surface, similar to FimA fimbriae. Longer Mfa1 fimbriae contributed to stronger autoaggregation of bacterial cells. Complementation of the mutant with the wild-type mfa2 allele in trans restored the parental phenotype. Mfa2 is present in the outer membrane of P. gingivalis, but does not co-purify with the Mfa1 fimbriae. However, co-immunoprecipitation demonstrated that Mfa2 and Mfa1 are associated with each other in whole P. gingivalis cells. Furthermore, immunogold microscopy, including double labelling, confirmed that Mfa2 was located on the cell surface and likely associated with Mfa1 fimbriae. Mfa2 may therefore play a role as an anchor for the Mfa1 fimbriae and also as a regulator of Mfa1 filament length. Two additional downstream genes (pgn0289 and pgn0290) are co-transcribed with mfa1 (pgn0287) and mfa2 (pgn0288), and proteins derived from pgn0289, pgn0290 and pgn0291 appear to be accessory fimbrial components.
Hasegawa
Yoshiaki
Y
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Iwami
Jun
J
Department of Endodontology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.|Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Sato
Keiko
K
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Park
Yoonsuk
Y
Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA.
Nishikawa
Kiyoshi
K
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Atsumi
Tatsuo
T
Department of Medical Technology, Gifu University of Medical Science, Seki, Gifu 501-3892, Japan.|Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Moriguchi
Keiichi
K
Department of Anatomy, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Murakami
Yukitaka
Y
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Lamont
Richard J
RJ
Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA.
Nakamura
Hiroshi
H
Department of Endodontology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Ohno
Norikazu
N
Department of Anatomy, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
Yoshimura
Fuminobu
F
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan.
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D036781:Fimbriae Proteins / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D005816:Genetic Complementation Test; D047468:Immunoprecipitation; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C545438:Mfa1 protein, Porphyromonas gingivalis; C545439:Mfa2 protein, Porphyromonas gingivalis; D037521:Virulence Factors; D036781:Fimbriae Proteins