[Study on the correlation between CD14 gene polymorphism and T cell-mediated immunity in severely burned patients].
OBJECTIVE
To investigate the correlation between CD14 gene polymorphism and T cell-mediated immunity in severely burned patients.


METHODS
The blood samples of 77 patients with extensive burn injury (> 30% total body surface area) were collected, and CD14-159C/T gene polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). T lymphocyte cell proliferation and interleukin-2 (IL-2) production were determined, and the ratio of CD4(+)/CD8(+) T lymphocyte as well as apoptosis of CD4(+) T lymphocyte was examined by flow cytometry.


RESULTS
The ability of T lymphocyte proliferation was obviously decreased in severely burned patients. Compared with CC homozygote patients, proliferative activity of T lymphocyte to mitogen stimulation was significantly depressed in TT and TC patients on post burn days 5, 21, and 28 (P < 0.05 or P < 0.01). IL-2 production in TT, TC patients was constantly in low level after burns, while it was increased from post burn day 14 in CC patients. The ratio of CD4(+)/CD8(+) T lymphocytes was markedly decreased in TC, TT patients than that in CC patients, especially on post burn days 1, 3, 14, 21, and 28 (P < 0.05 or P < 0.01). Meanwhile, compared with CC homozygote patients, the apoptosis rates of CD3(+)CD4(+) T lymphocytes were much higher in TT patients on post burn days 5, 7, and 21 (P < 0.05), and in TC patients on days 7, 14 (P < 0.05), respectively. However, no obvious differences in parameters of immune function of T lymphocytes were found between TT and TC patients (P > 0.05).


CONCLUSIONS
CD14-159C/T polymorphism could influence the T cell-mediated immunity in extensively burned patients, which might participate in the development of septic complications secondary to major burns.
Dong
Ning
N
Burns Institute, First Hospital Affiliated to the General Hospital of the People's Liberation Army, Beijing 100037, China.
Yao
Yong-ming
YM
Yu
Yan
Y
Cao
Yu-jue
YJ
He
Li-xin
LX
Yang
Hong-ming
HM
Sheng
Zhi-yong
ZY
D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D002056:Burns / Q000235:genetics / Q000276:immunology*; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D055815:Young Adult
D007376:Interleukin-2; D018950:Lipopolysaccharide Receptors