Actin-like filaments in synaptosomes detected by heavy meromyosin binding.
We have prepared cat forebrain synaptosomes in order to investigate the presence of actin-like proteins. First, actin-like filaments were demonstrated using a heavy meromyosin binding technique. Decorated filaments form networks inside the synaptosomes and are frequently seen in relation to membranes and synaptic vesicles. Second, using SDS-polyacrylamide gel electrophoresis, a band corresponding to actin appears to be one of the major constituents of synaptosomal fractions; this confirms the work of Fine and his coworkers. The significance of actin-like filaments in relation to axoplasmic transport is briefly discussed.
Inestrosa
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Department of Cell Biology, Catholic University of Chile, P.O. Box 114-D, Santiago Chile; Laboratory of Neurophysiology, Catholic University of Chile, P.O. Box 114-D, Santiago Chile.
Fernandez
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HL
Garrido
J
J