Phylogenetic analysis of Mycoplasma suis isolates based on 16S rRNA gene sequence in China.
To perform phylogenetic analysis of Mycoplasma suis isolates derived from China to define the nature of this pathogen, nearly complete of 16S rRNA genes from Chongqing, Sichuan, Henan and Guangdong isolates were amplified by PCR and sequenced. The four sequences from the blood samples in this study, with other 17 Hemoplasmas sequences and related 3 mycoplasma sequences available in the GenBank, were aligned using Clustal X (version 1.83) sequences alignment program. Maximum parsimony, neighbor-joining and minimum evolution (MEGA 4.0) algorithms were used to create phylogenetic trees. Phylogenetic analysis of these sequences showed that all hemoplasma species were located within a single clade and were most closely related to M. pneumoniae group. The hemoplasma species were further subdivided into two distinct groups, one containing M.wenyonii, M.suis and Candidatus M. haemominutum and the other containing M. haemofelis and M. haemocanis. Within the former clade, four M.suis isolates from Mainland China and other M.suis species formed a monophyletic group in the tree. A tendency of clear geographical grouping of the isolate was evident.
Zhou
Rong-Qiong
RQ
Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, The People's Republic of China.
Nie
Kui
K
Huang
Han-Cheng
HC
Hu
Shi-jun
SJ
Zhou
Zuo-yong
ZY
Luo
Hong-Lin
HL
D000818:Animals; D002681:China; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine / Q000382:microbiology
D012336:RNA, Ribosomal, 16S