Effects of endotoxin and catecholamines on hepatic mitochondrial respiration.
Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.
Porta
Francesca
F
Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, Bern, Switzerland.
Bracht
Hendrik
H
Weikert
Christian
C
Beck
Mario
M
Takala
Jukka
J
Brandt
Sebastian
S
Hiltebrand
Luzius B
LB
Jakob
Stephan M
SM
D000244:Adenosine Diphosphate / Q000032:analysis / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology*; D019069:Cell Respiration / Q000502:physiology; D004731:Endotoxins / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D013552:Swine; D013997:Time Factors
D002395:Catecholamines; D004731:Endotoxins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate