The effect of tissue culture on suture holding strength and degradation in canine tendon.
The purpose of this study was to assess tendon metabolism and suture pull-out strength after simple tendon suture in a tissue culture model. One hundred and twelve flexor digitorum profundus tendons from 28 dogs were cultured for 7, 14, or 21 days with or without a static tensile load. In both groups increased levels of matrix metalloproteinase (MMP) mRNA was noted. Suture pull-out strength did not decrease during tissue culture. While the presence of a static load had no effect on the pull-out strength, it did affect MMP mRNA expression. This tissue culture model could be useful in studying the effect of factors on the tendon-suture interface.
Omae
H
H
Orthopedic Biomechanics Laboratory, Mayo Clinic Rochester, MN 55905, USA.
Zhao
C
C
Sun
Y-L
YL
Zobitz
M E
ME
Moran
S L
SL
Amadio
P C
PC
D000818:Animals; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D004285:Dogs; D005552:Forelimb; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013537:Sutures; D013710:Tendons / Q000378:metabolism* / Q000503:physiopathology* / Q000601:surgery; D013718:Tensile Strength; D046509:Tissue Culture Techniques; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism; D016474:Weight-Bearing
D024042:Collagen Type I; D024061:Collagen Type III; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases