title: Black shank resistant tobacco by silencing of glutathione S-transferase.
abstract: A glutathione S-transferase gene was amplified from cDNA of Nicotiana tabacum roots infected with Phytophthora parasitica var. nicotianae. The gene was cloned in sense and anti-sense orientation to an RNAi vector for induced gene silencing, and reduced expression of the gene was detected by RT-PCR. A statistically significant increase in resistance of N. tabacum to infection following gene silencing was found for glutathione S-transferase-silenced plants compared with control plants. Some defense genes were up-regulated in glutathione S-transferase-silenced plants during the interaction with the pathogen. This is the first evidence of the role of glutathione S-transferase as negative regulator of defense response.
authors: Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Havana 10600, Cuba.
authors: Ingrid
authors: I
authors: Hernández
authors: Osmany
authors: O
authors: Chacón
authors: Raisa
authors: R
authors: Rodriguez
authors: Roxana
authors: R
authors: Portieles
authors: Yunior
authors: Y
authors: López
authors: Merardo
authors: M
authors: Pujol
authors: Orlando
authors: O
authors: Borrás-Hidalgo
mesh_terms: D003001:Cloning, Molecular; D020868:Gene Silencing; D005982:Glutathione Transferase / Q000235:genetics*; D010838:Phytophthora; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D014026:Nicotiana / Q000235:genetics*
chemical_list: D005982:Glutathione Transferase