title: Molecular alterations associated with aneurysmal remodeling are localized in the high hemodynamic stress region of a created carotid bifurcation.
abstract: OBJECTIVE
  Although elevated hemodynamics has been speculated to play a key role in intracranial aneurysm (IA) initiation, little is known about the specific hemodynamic microenvironment that triggers aneurysmal vascular degradation. We previously demonstrated maladaptive remodeling characteristic of IA initiation occurring in hemodynamic regions of combined high wall shear stress (WSS) and high WSS gradient near the apex of an experimentally created carotid bifurcation. This study examines whether this remodeling recapitulates the molecular changes found in IAs and whether molecular changes also correspond to specific hemodynamic environments.
  
  
  METHODS
  De novo bifurcations were surgically created using both native common carotid arteries in each of 6 dogs. Bifurcations were imaged 2 weeks or 2 months after surgery by high-resolution 3-dimensional angiography, from which flow fields were obtained by computational fluid dynamics simulations. Subsequently, harvested tissues, demonstrating early aneurysmal changes near the apex, were immunostained for interleukin-1beta, endothelial and inducible nitric oxide synthases, nitrotyrosine, and matrix metalloproteinase-2 and -9. Spatial distributions of these molecules were comapped with computational fluid dynamics results.
  
  
  RESULTS
  The aneurysmal wall showed decreased endothelial nitric oxide synthase expression compared with surrounding segments, the feeding artery, and native controls, whereas all other markers increased. Anti-CD68 staining indicated the absence of inflammatory cells in the aneurysmal wall. Comapping molecular marker distributions with flow fields revealed confinement of these molecular changes within the hemodynamic region of high WSS and high, positive WSS gradient.
  
  
  CONCLUSIONS
  Aneurysm-initiating remodeling induced by combined high WSS and high, positive WSS gradient is associated with molecular changes implicated in IAs.
authors: Department of Mechanical and Aerospace Engineering, University at Buffalo, State University of New York, Buffalo, New York 14214, USA.
authors: Zhijie
authors: Z
authors: Wang
authors: John
authors: J
authors: Kolega
authors: Yiemeng
authors: Y
authors: Hoi
authors: Ling
authors: L
authors: Gao
authors: Daniel D
authors: DD
authors: Swartz
authors: Elad I
authors: EI
authors: Levy
authors: J
authors: J
authors: Mocco
authors: Hui
authors: H
authors: Meng
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017536:Carotid Artery, Common / Q000601:surgery; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006439:Hemodynamics; D002532:Intracranial Aneurysm / Q000378:metabolism* / Q000503:physiopathology* / Q000662:veterinary; D018810:Magnetic Resonance Angiography; D008279:Magnetic Resonance Imaging; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D013314:Stress, Mechanical; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: C002744:3-nitrotyrosine; D014443:Tyrosine; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9