Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus.
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which causes significant economic losses to the pig industry worldwide. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. This study was aimed to develop a recombinant human adenovirus type 5 expressing the CSFV E2 gene (rAdV-E2) and evaluate its efficacy in rabbits and pigs. The results showed that the rabbits and the pigs immunized with the rAdV-E2 developed high-level CSFV-specific neutralizing antibodies. The rAdV-E2-immunized rabbits were protected from fever induced by infection with C-strain, which is pathogenic to the rabbit, and the rAdV-E2-immunized pigs were protected from lethal challenge with highly virulent Shimen strain. This indicates that the recombinant adenovirus can be an attractive candidate vaccine for preventing CSF.
Sun
Yuan
Y
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Liu
Da-Fei
DF
Wang
Yu-Fei
YF
Liang
Bing-Bing
BB
Cheng
Dan
D
Li
Na
N
Qi
Qiao-Fen
QF
Zhu
Qing-Hu
QH
Qiu
Hua-Ji
HJ
D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors / Q000235:genetics; D011817:Rabbits / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000276:immunology / Q000821:virology; D014614:Vaccines, Synthetic / Q000276:immunology / Q000627:therapeutic use*; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology
D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus