title: Hybrid silica polymeric monolith-based in-tube microextraction and CE for determination of bisphenol A in beverages.
abstract: On-line concentration techniques in capillary electrophoresis, including stacking and sweeping, have a weak tolerance towards complex real samples. A phenyl-functionalized hybrid silica monolithic polymeric capillary column (id 530 microm) was prepared by in-situ co-condensation of phenyltriethoxysilane (PTES) and tetraethoxysilane (TEOS). In order to enhance the stability of a monolithic column within a wide-bore capillary, a novel and simple frit-preparation method for wide-bore capillaries was first introduced by immobilizing a short length of capillary of suitable outer diameter at the end of the monolithic capillary column during condensation of the sol-gel solution. The monolithic column was used for the extraction of bisphenol A (BPA) from cola samples, and the interfering compounds can be removed from the samples with 40% ACN solution (v/v) as eluent. The pretreated samples were further concentrated and separated using large-volume stacking and sweeping micellar electrokinetic chromatography. The method was successfully applied to the determination of bisphenol A spiked in cola, yielding a detection limit of 1.8 ng/mL. Close correlation coefficients and acceptable method reproducibility were obtained for bisphenol A over a linear range of 0.005-0.200 microg/mL.
authors: College of Food Science & Technology, State Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, China.
authors: Jiwei
authors: J
authors: Hu
authors: Xuegang
authors: X
authors: Li
authors: Yawen
authors: Y
authors: Cai
authors: Heyou
authors: H
authors: Han
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D001628:Beverages / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D011108:Polymers; D012822:Silicon Dioxide; C006780:bisphenol A; D000098826:Bisphenol A Compounds