Comparisons between geographically diverse samples of carried Staphylococcus aureus.
Approximately one-third of the human population is asymptomatically colonized by Staphylococcus aureus. However, much of the global diversity within the carriage populations remains uncharacterized, and it is unclear to what degree the variation is geographically partitioned. We isolated 300 carriage isolates from 1,531 adults contemporaneously in four countries: France, Algeria, Moldova, and Cambodia. All strains were characterized by multilocus sequence typing. Six clonal complexes (CCs) were present in all four samples (CC30, -45, -121, -15, -5, and -8). Analyses based on the genotype frequencies revealed the French and Algerian samples to be most similar and the Cambodian sample to be most distinct. While this pattern is consistent with likely rates of human migration and geographic distance, stochastic clonal expansion also contributes to regional differences. Phylogenetic analysis revealed a highly divergent and uncharacterized genotype (ST1223) within Cambodia. This lineage is related to CC75, which has previously been observed only in remote aboriginal populations in northern Australia.
Ruimy
Raymond
R
Laboratoire Associé Centre National de Référence Résistance dans les Flores Commensales, Hôpital Bichat-Claude Bernard (AP-HP), 75018 Paris, France. raymond.ruimy@bch.ap-hop-paris.fr
Armand-Lefevre
Laurence
L
Barbier
Francois
F
Ruppé
Etienne
E
Cocojaru
Radu
R
Mesli
Yasmine
Y
Maiga
Aminata
A
Benkalfat
Mokhtar
M
Benchouk
Samia
S
Hassaine
Hafida
H
Dufourcq
Jean-Baptiste
JB
Nareth
Chhor
C
Sarthou
Jean-Louis
JL
Andremont
Antoine
A
Feil
Edward J
EJ
D000328:Adult; D000462:Algeria / Q000453:epidemiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002160:Cambodia / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008966:Moldova / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; D004269:DNA, Bacterial