title: Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555.
abstract: The lipopolysaccharide of Citrobacter freundii O22 (strain PCM 1555) was degraded under mild acidic conditions and the O-polysaccharide released was isolated by gel chromatography. Sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H ROESY and (1)H,(13)C HMBC experiments, showed that the repeating unit of the O-polysaccharide has the following structure: alpha-Abep 1 -->3 --> 2)-alpha-D-Manp-(1-->4)-alpha-L-Rhap-(1-->3)-alpha-D-Galp-(1--> where Abe is abequose (3,6-dideoxy-D-xylo-hexose). SDS-PAGE and immunoblotting revealed that the O-antigen of C. freundii O22 is serologically indistinguishable from those of Salmonella group B serovars (Typhimurium, Brandenburg, Sandiego, Paratyphi B) but not related to other abequose-containing O-antigens tested (Citrobacter werkmanii O38 and Salmonella Kentucky) or colitose (l enantiomer of abequose)-containing O-antigen of Escherichia coli O111.
authors: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland. katzenel@iitd.pan.wroc.pl
authors: Ewa
authors: E
authors: Katzenellenbogen
authors: Nina A
authors: NA
authors: Kocharova
authors: Philip V
authors: PV
authors: Toukach
authors: Sabina
authors: S
authors: Górska
authors: Agnieszka
authors: A
authors: Korzeniowska-Kowal
authors: Maria
authors: M
authors: Bogulska
authors: Andrzej
authors: A
authors: Gamian
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D016971:Citrobacter freundii / Q000737:chemistry* / Q000145:classification; D004591:Electrophoresis, Polyacrylamide Gel; D006601:Hexoses / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D012475:Salmonella / Q000737:chemistry
chemical_list: D006601:Hexoses; D019081:O Antigens; C023708:abequose