title: Gene expression of halophyte Kosteletzkya virginica seedlings under salt stress at early stage.
abstract: Gene differential expression of Kosteletzkya virginica seedlings under salt stress at two time points (2, 24 h) in roots and leaves was analyzed using the cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. Polymorphic transcript-derived fragments (TDFs) among control plants and salt-treated plants were grouped into four main differential expression patterns: repression (A), de novo induction (B), up-regulation (C) and down-regulation (D). Among them, 34 differentially expressed gene fragments were homologous to known genes from other species and 4 were sequences with unknown functions. These differentially expressed genes can be classified into four groups according to their putative functions: (1) genes for re-establishing ion homeostasis and protecting the plant from stress damage; (2) genes involved in metabolism or energy and resuming plant growth and development under salt stress; (3) genes involved in regulation of gene expression; (4) genes for signal transduction. Changes of eight differentially expressed genes were confirmed by quantitative real time RT-PCR.
authors: Halophyte Research Lab., Nanjing University, 210093 Nanjing, China.
authors: Yu-Qi
authors: YQ
authors: Guo
authors: Zeng-Yuan
authors: ZY
authors: Tian
authors: Guang-Yong
authors: GY
authors: Qin
authors: Dao-Liang
authors: DL
authors: Yan
authors: Jie
authors: J
authors: Zhang
authors: Wen-Zong
authors: WZ
authors: Zhou
authors: Pei
authors: P
authors: Qin
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D019660:Malvaceae / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000378:metabolism*; D012965:Sodium Chloride; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012965:Sodium Chloride