Tenomodulin is highly expressed in adipose tissue, increased in obesity, and down-regulated during diet-induced weight loss.
BACKGROUND
Tenomodulin (TNMD), a putative angiogenesis inhibitor, is expressed in hypovascular connective tissues. Global gene expression scans show that the TNMD gene also is expressed in human adipose tissue and that its expression is regulated in response to weight reduction; however, more detailed information is lacking.


OBJECTIVE
The aim of this study was to investigate TNMD tissue distribution and TNMD gene expression in human adipose tissue in relation to obesity and metabolic disease.


METHODS
TNMD gene expression, tissue distribution, and TNMD gene expression in adipose tissue from different depots, from lean and obese subjects, and during diet-induced weight reduction were analyzed by DNA microarray and real-time PCR.


METHODS
We primarily measured TNMD gene expression.


RESULTS
The TNMD gene was predominantly expressed in sc adipose tissue. TNMD gene expression was higher in sc than omental adipose tissue both in lean (P = 0.002) and obese subjects (P = 0.014). In both women and men, TNMD gene expression was significantly higher in the obese subjects compared to the lean subjects (P = 1.1 x 10(-26) and P = 0.010, respectively). In a multiple linear regression analysis, BMI was a significant independent predictor of TNMD gene expression. TNMD gene expression was down-regulated during diet-induced weight loss, with a 65% decrease after 18 wk of diet (P < 0.0001).


CONCLUSIONS
We conclude that human adipose tissue TNMD gene expression is highly affected by obesity, adipose tissue location, and weight loss, indicating that TNMD may play a role in adipose tissue function.
Saiki
Atsuhito
A
Sahlgrenska Center for Cardiovascular and Metabolic Research, Department of Molecular and Clinical Medicine, The Sahlgrenska Academy at Gothenburg University, 413 45 Gothenburg, Sweden.
Olsson
Maja
M
Jernås
Margareta
M
Gummesson
Anders
A
McTernan
Philip G
PG
Andersson
Johanna
J
Jacobson
Peter
P
Sjöholm
Kajsa
K
Olsson
Bob
B
Yamamura
Shigeo
S
Walley
Andrew
A
Froguel
Philippe
P
Carlsson
Björn
B
Sjöström
Lars
L
Svensson
Per-Arne
PA
Carlsson
Lena M S
LM
D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000368:Aged; D015992:Body Mass Index; D004038:Diet, Reducing; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013548:Sweden; D015431:Weight Loss
D008565:Membrane Proteins; C422444:TNMD protein, human