title: Juvenile sea bass (Dicentrarchus labrax L.) enzymatic and non-enzymatic antioxidant responses following 17beta-estradiol exposure.
abstract: In the context of 17beta-estradiol (E2) as an environmental contaminant, this study was designed to test the hypothesis whether it can modulate antioxidant defenses in Dicentrarchus labrax, taking gills as the target organ. Enzymatic (GPX--glutathione peroxidase; CAT--catalase; GR--glutathione reductase; GST--glutathione S-transferase) and non-enzymatic antioxidants (NP-SH--non protein thiols; GSHt--total glutathione) were measured following 10-day exposure to E2 in two different ways: water diluted (WD, 200 or 2,000 ng/L) and intraperitoneally injected (i.p., 0.5 or 5 mg/kg). WD exposure caused a single alteration-CAT increase, whereas i.p. exposure decreased all the enzymatic antioxidants. Similarly, NP-SH and GSHt were reduced by i.p. exposure. Thus, different E2 exposure routes determined clear differences on the assessed responses. Despite gills close contact with water, their defenses were not strongly affected in WD experiment. Differently, i.p. injected fish showed an overall decrease in both enzymatic and non-enzymatic antioxidants, more pronounced at the highest concentration, pointing out the E2 oxidative stress inducing potential in fish.
authors: CESAM & Department of Biology, University of Aveiro, 3810-193, Aveiro, Portugal. ahmadr@ua.pt
authors: Iqbal
authors: I
authors: Ahmad
authors: Vera Lúcia
authors: VL
authors: Maria
authors: Mário
authors: M
authors: Pacheco
authors: Maria Ana
authors: MA
authors: Santos
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001492:Bass; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000633:toxicity*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007274:Injections, Intraperitoneal; D018384:Oxidative Stress; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D014874:Water Pollutants, Chemical; D004958:Estradiol; D005978:Glutathione