title: Heteronuclear and homonuclear Cu2+ and Zn2+ complexes with multihistidine peptides based on zebrafish prion-like protein.
abstract: The homeostasis of metal ions, especially copper and zinc, is a major factor that may influence the prion diseases and the biological function of prion protein (PrP). The His-rich regions are basic sites for metal binding and antioxidant activity of the PrP structures. Animal prion-like proteins contain also His-rich domains, and their coordination chemistry may provide better insight into the chemistry and biology of PrP structures and related diseases. Herein, we report an equilibrium study on heteronuclear Zn(2+)-Cu(2+) complexes with zrel-PrP fragments from zebrafish. Potentiometric, spectroscopic, and mass spectrometric methods showed that the binding of copper is much more effective than the binding of zinc. At physiological pH, both metals bind to the histidine imidazole N donors of the studied peptides.
authors: Department of Chemistry, University of Siena, Via Aldo Moro, 53100 Siena, Italy. valensindan@unisi.it
authors: Daniela
authors: D
authors: Valensin
authors: Łukasz
authors: Ł
authors: Szyrwiel
authors: Francesca
authors: F
authors: Camponeschi
authors: Magdalena
authors: M
authors: Rowińska-Zyrek
authors: Elena
authors: E
authors: Molteni
authors: Elzbieta
authors: E
authors: Jankowska
authors: Aneta
authors: A
authors: Szymanska
authors: Elena
authors: E
authors: Gaggelli
authors: Gianni
authors: G
authors: Valensin
authors: Henryk
authors: H
authors: Kozłowski
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D006639:Histidine / Q000737:chemistry / Q000378:metabolism; D007536:Isomerism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011328:Prions / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D015027:Zebrafish / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010455:Peptides; D011328:Prions; D006639:Histidine; D003300:Copper; D015032:Zinc