title: Bone marrow cell therapy prevents infarct expansion and improves border zone remodeling after coronary occlusion in rats.
abstract: BACKGROUND
  Since the cell therapy benefits for myocardial infarction are mainly related to infarct reduction by regenerating lost myocardium or increasing survival of tissues at risk, we evaluated the effects of bone marrow-derived mononuclear cells (MNC), implanted after the completion of necrosis, on infarct progression and cardiac remodeling.
  
  
  METHODS
  After 48 h of induction of myocardial infarction (MI), Lewis-inbred rats were injected with 6 × 10(6) cells (MI+MNC) or saline (MI). After six weeks, scar dimension, ventricular morphology and function were analyzed by echocardiography followed by histomorphology of the infarcted and border zones.
  
  
  RESULTS
  After therapy, the relative size of the infarct was smaller in MI+MNC (37 ± 1% of the left ventricle) than in MI (43 ± 1%). While the MI group exhibited parallel elongation of the infarcted (31.6 ± 3.8% increase) and reminiscent ventricular portions (33.5 ± 3.7%), MNC therapy preserved the initial infarct length. Infarcted walls were thicker (979 ± 31 mm) in the MNC group than in the untreated group (709 ± 41 mm), also demonstrating an absence of infarct expansion. In the border zones, MNC led to increased capillary densities and capillary/myocyte ratios. The cardiac systolic function remained depressed in MI, but improved by 19 ± 5% in MI+MNC which reduced the incidence of pulmonary arterial hypertension (37.5% in MI and 6.25% in MI+MNC).
  
  
  CONCLUSIONS
  MNC therapy prevented the infarct expansion and thinning related to cardiac remodeling and was associated with an improvement of border zone microcirculation: as a result, MNC therapy reduced typical MI dysfunctional repercussions.
authors: Cardiology Division, Department of Medicine, Federal University of Sao Paulo, Brazil, SP 04024-900, Brazil. leodossantos@hotmail.com
authors: Leonardo
authors: L
authors: dos Santos
authors: Alexandra A
authors: AA
authors: Santos
authors: Giovana A
authors: GA
authors: Gonçalves
authors: José Eduardo
authors: JE
authors: Krieger
authors: Paulo José Ferreira
authors: PJ
authors: Tucci
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000379:methods / Q000473:pathology; D054059:Coronary Occlusion / Q000150:complications / Q000601:surgery*; D005260:Female; D009203:Myocardial Infarction / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control*; D051381:Rats; D011917:Rats, Inbred Lew; D020257:Ventricular Remodeling / Q000502:physiology