[Regulation of immune suppressive cytokines by TLR4 activation in colon cancer cells].
OBJECTIVE
To investigate the regulation of Toll-like receptors (TLRs) on immune suppressive cytokines in situ colon cancer cells.


METHODS
The mRNA and protein expression spectrum of TLRs in HT-29 cells were determined by RT-PCR and Western blot respectively. The cytokines and chemokines levels of supernant of HT-29 stimulated by lipoplysaccharide(LPS) were detected with ELISA.


RESULTS
TLR1-9 were expressed in HT-29 cells on mRNA level. After LPS stimulation, TLR4 mRNA and protein expressions were up-regulated in HT-29 cells, and TGF-beta, VEGF, IL-8, CCL20 and IL-6 levels increased significantly(all P<0.01). Except IL-6, up-regulation of the other cytokines was not suppressed by NF-kappa B inhibitor.


CONCLUSIONS
TLRs expressed on colon cancer cells may elevate the immune suppressive cytokines and chemokines, which promote the immune escape of cancer cells.
Qi
Hong-ming
HM
Department of Surgery, The People's Hospital of Zhuji, Zhejiang Zhuji 311800, China. qhm13587373953@126.com
Zhu
Tie-ming
TM
Wang
Jian
J
D054418:Chemokine CCL20 / Q000378:metabolism; D003110:Colonic Neoplasms / Q000276:immunology* / Q000378:metabolism*; D016207:Cytokines / Q000276:immunology*; D019073:HT29 Cells; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000276:immunology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015854:Up-Regulation
C108355:CCL20 protein, human; D054418:Chemokine CCL20; D016207:Cytokines; D015850:Interleukin-6; D016328:NF-kappa B; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D016212:Transforming Growth Factor beta