Development of high performance liquid chromatography method for buspirone in rabbit serum: Application to pharmacokinetic study.
A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification of buspirone (BUSP) in rabbit serum was developed and validated. BUSP and internal standard (IS), diltiazem hydrochloride were extracted into dichloromethane and separated using an isocratic mobile phase, on a Kromasil C(8) column. The eluent was monitored by UV detector at 235 nm and at a flow rate of 1.0 mL min(-1). The linearity range of proposed method was 1-3000 ng mL(-1). The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 97 and 96% for BUSP and IS, respectively. The method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of buspirone after application of reservoir based transdermal therapeutic system of BUSP to rabbits.
Gannu
Ramesh
R
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal 506009, Andhra Pradesh, India.
Yamsani
Shravan Kumar
SK
Palem
Chinna Reddy
CR
Yamsani
Vamshi Vishnu
VV
Kotagiri
Harshini
H
Yamsani
Madhusudan Rao
MR
D000818:Animals; D014151:Anti-Anxiety Agents / Q000097:blood* / Q000493:pharmacokinetics; D002065:Buspirone / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011817:Rabbits; D012680:Sensitivity and Specificity
D014151:Anti-Anxiety Agents; D002065:Buspirone