Novel monoclonal antibodies recognise guinea fowl thrombocytes.
This paper introduces two novel monoclonal antibodies, designated GTr1 and GTr2, which recognise guinea fowl thrombocyte surface antigen(s). The antibodies were tested in embryos and adult birds. GTr1 and GTr2 staining emerged at embryonic days 12 and 7, respectively. After embryonic day 12 there was no difference in staining pattern between the two monoclonal antibodies. The isotype of the antibodies is IgG1. The antibodies did not react with any other haematopoietic cells of guinea fowl, and there was no species cross-reaction with chicken, turkey and quail. The antibodies can be used in interspecies chimeric and parabiotic experiments to identify cells of guinea fowl origin.
Bódi
Ildikó
I
Semmelweis University, Department of Human Morphology and Developmental Biology, Faculty of Medicine, Tuzoltó u. 58 H-1094 Budapest, Hungary.
Nagy
Nándor
N
Sinka
Lídia
L
Igyártó
Botond-Zoltán
BZ
Oláh
Imre
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface; D001792:Blood Platelets / Q000166:cytology / Q000276:immunology*; D002645:Chickens / Q000276:immunology; D046094:Galliformes / Q000097:blood*; D011784:Quail / Q000276:immunology; D014422:Turkeys / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface