Bacillus acidiproducens sp. nov., vineyard soil isolates that produce lactic acid.
Two novel spore-forming lactic acid bacteria, strains SL213T and SL1213, were isolated from vineyard soils in Korea. Cells of both isolates were rod-shaped bacilli and contained subterminal, ellipsoidal spores. Strains were facultatively anaerobic, catalase-positive, oxidase-negative and motile with single flagella. meso-Diaminopimelic acid, glucose and galactose were detected in whole-cell hydrolysates. Major fatty acids found in the strains were anteiso-C15:0, iso-C15:0, iso-C16:0, C16:0 and anteiso-C17:0. The G+C contents of the DNA were 46.1 and 46.3 mol%. 16S rRNA gene sequences from the two strains were almost identical (99.9%) and placed them in the genus Bacillus, according to phylogenetic analysis. The type strains most closely related to SL213T were Bacillus coagulans ATCC 7050T and Bacillus badius ATCC 14574T, with 16S rRNA gene sequence similarities of 96.9 and 95.9%, respectively. Levels of DNA-DNA relatedness between strain SL213T and strain SL1213, B. coagulans ATCC 7050T and B. badius ATCC 14574T were 92.5, 49.0 and 27.5%, respectively. On the basis of 16S rRNA gene sequences and chemotaxonomic and phenotypic evidence given in this study, we report that SL213T represents a novel species, for which the name Bacillus acidiproducens sp. nov. is proposed. The type strain is SL213T (=KCTC 13078T=JCM 14638T).
Jung
Min Young
MY
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Daejeon 305-806, Republic of Korea.
Kim
Joong-Su
JS
Chang
Young-Hyo
YH
D000332:Aerobiosis; D000693:Anaerobiosis; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D007723:Korea; D019344:Lactic Acid / Q000378:metabolism*; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D003960:Diaminopimelic Acid; D010088:Oxidoreductases; D002374:Catalase; D005947:Glucose; D005690:Galactose