title: Expression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae pseudopilin EpsH.
abstract: EpsH is a minor pseudopilin protein of the Vibrio cholerae type II secretion system. A truncated form of EpsH with a C-terminal noncleavable His tag was constructed and expressed in Escherichia coli, purified and crystallized by sitting-drop vapor diffusion. A complete data set was collected to 1.71 A resolution. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 53.39, b = 71.11, c = 84.64 A. There were two protein molecules in the asymmetric unit, which gave a Matthews coefficient V(M) of 2.1 A(3) Da(-1), corresponding to 41.5% solvent content.
authors: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
authors: Kannan
authors: K
authors: Raghunathan
authors: Frank S
authors: FS
authors: Vago
authors: Terry
authors: T
authors: Ball
authors: Nafissa
authors: N
authors: Yakubova
authors: David
authors: D
authors: Grindem
authors: William J
authors: WJ
authors: Wedemeyer
authors: Dennis N
authors: DN
authors: Arvidson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D014734:Vibrio cholerae / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins