A novel mosquitocidal Bacillus thuringiensis strain LLP29 isolated from the phylloplane of Magnolia denudata.
Eleven Bacillus thuringiensis isolates were recovered from phylloplanes of Magnolia denudata, a specific source of new strains of B. thuringiensis. Among these, a new strain, LLP29, was found to be most toxic to mosquitoes based on the results of preliminary toxicity analysis. Phase contrast microscopy, mosquitocidal activity, polymerase chain reaction (PCR) analysis and parasporal inclusion were performed to learn more about the characteristics of this novel mosquitocidal isolate. The LC(50) values of LLP29 against Aedes albopictus and Culex quinquefasciatus were 0.33 and 0.04 ng of protein/ml, respectively. The cyt1 gene, which encodes the Cyt protein that is toxic to mosquitoes, was subsequently detected, cloned, sequenced and expressed in acrystalliferous Bt HD73 Cry(-). The results indicated that it might be a member of the cyt1Aa gene group. The novel strain LLP29 appears to be a new subspecies of B. thuringiensis and should prove useful in the control of mosquitoes and mosquito-borne diseases.
Zhang
Lingling
L
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, People's Republic of China.
Huang
Enjiong
E
Lin
Jing
J
Gelbic
Ivan
I
Zhang
Qunlin
Q
Guan
Yi
Y
Huang
Tianpei
T
Guan
Xiong
X
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D003465:Culex / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007300:Insect Control / Q000379:methods*; D007306:Insecticides / Q000378:metabolism; D007928:Lethal Dose 50; D031566:Magnolia / Q000382:microbiology*; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D010572:Pest Control, Biological / Q000379:methods*; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016019:Survival Analysis
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D007306:Insecticides