Factors affecting ferulic acid release from Brewer's spent grain by Fusarium oxysporum enzymatic system.
In this study, the factors affecting ferulic acid (FA) release from Brewer's spent grain (BSG), by the crude enzyme extract of Fusarium oxysporum were investigated. In order to evaluate the importance of the multienzyme preparation on FA release, the synergistic action of feruloyl esterase (FAE, FoFaeC-12213) and xylanase (Trichoderma longibrachiatum M3) monoenzymes was studied. More than double amount of FA release (1 mg g(-1) dry BSG) was observed during hydrolytic reactions by the crude enzyme extract compared to hydrolysis by the monoenzymes (0.37 mg g(-1) dry BSG). The protease content of the crude extract and the inhibitory effect of FA as an end-product were also evaluated concerning their effect on FA release. The protease treatment prior to hydrolysis by monoenzymes enhanced FA release about 100%, while, for the first time in literature, FA in solution found to have a significant inhibitory effect on FAE activity and on total FA release.
Xiros
Charilaos
C
BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, Athens 15780, Greece.
Moukouli
Maria
M
Topakas
Evangelos
E
Christakopoulos
Paul
P
D001709:Biotechnology / Q000379:methods; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry*; D002523:Edible Grain / Q000378:metabolism*; D005285:Fermentation; D005670:Fusarium / Q000378:metabolism*; D006868:Hydrolysis; D007218:Industrial Microbiology; D007220:Industrial Waste; D010447:Peptide Hydrolases / Q000737:chemistry; D018514:Plant Structures / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods; D014242:Trichoderma / Q000201:enzymology; D043325:Xylan Endo-1,3-beta-Xylosidase / Q000737:chemistry
D003373:Coumaric Acids; D007220:Industrial Waste; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D043325:Xylan Endo-1,3-beta-Xylosidase; D010447:Peptide Hydrolases