The GroEL/GroES cis cavity as a passive anti-aggregation device.
The GroEL/GroES chaperonin folding chamber is an encapsulated space of approximately 65 A diameter with a hydrophilic wall, inside of which many cellular proteins reach the native state. The question of whether the cavity wall actively directs folding reactions or is playing a passive role has been open. We review past and recent observations and conclude that the chamber functions as a passive "Anfinsen cage" that prevents folding monomers from multimolecular aggregation.
Horwich
Arthur L
AL
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA. arthur.horwich@yale.edu
Apetri
Adrian C
AC
Fenton
Wayne A
WA
D000199:Actins / Q000378:metabolism; D036022:Capsid Proteins / Q000378:metabolism; D018835:Chaperonin 10 / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D017510:Protein Folding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D036022:Capsid Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D011506:Proteins; D014404:Tubulin; C064517:gp23 protein, Bacteriophage T4