title: Effect of PVP on sperm capacitation status and embryonic development in cattle.
abstract: The objective of this study was to investigate the effects of polyvinylpyrrolidone (PVP) on sperm capacitation status and embryonic development in cattle (Bos taurus). Acrosome-reacted sperm (chlortetracycline stain) and the fertilization rate after intracytoplasmic sperm injection (ICSI) were enhanced (P<0.05) when sperm were cultured in a medium containing 10% PVP. However, injection of bovine in vitro-produced (IVP) embryos with medium containing 10% PVP suppressed development of these embryos to the 2-cell, morula, and blastocyst stages and cell number at the blastocyst stage (P<0.01) but did not affect chromosomal integrity (P>0.05). Embryo developmental capacity differed (P<0.01) among PVP from three suppliers, but there were no significant differences among three solvent media. The PVP remained localized in 40.9% of PVP-injected IVP embryos. In conclusion, PVP affected the acrosome reaction and enhanced the fertilization rate after ICSI. However, PVP remained detectable in IVP embryos and suppressed their developmental capacity.
authors: Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Japan.
authors: Y
authors: Y
authors: Kato
authors: Y
authors: Y
authors: Nagao
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D002417:Cattle / Q000196:embryology / Q000502:physiology; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D008297:Male; D011205:Povidone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D011247:Pregnancy; D013075:Sperm Capacitation / Q000187:drug effects*; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects
chemical_list: C077842:polyvinylpolypyrrolidone; D011205:Povidone