Effect of bone marrow stromal cell-conditioned medium on the glutamate uptake of peroxide-injured astrocytes.
We aimed to investigate the effect of bone marrow stromal cell-conditioned medium (BCM) on glutamate uptake of peroxide (H(2)O(2))-injured astrocytes. Bone marrow stromal cells (BMSC) were isolated from rat bone marrow. Confluent BMSC cultures were incubated with serum-free Dulbecco's Modified Eagle's Medium to create the BCM. Astrocytes were isolated from 1-day-old rats. H(2)O(2)-injured astrocytes were cultured in BCM (experimental group) or serum-free medium (control group). The labeled glutamate ((3)H-L-glutamate) uptake by H(2)O(2)-injured astrocytes with or without BCM was compared after 1 and 3 days. We found that astrocytes cultured in BCM exhibited increased glutamate uptake compared to those cultured in serum-free medium following H(2)O(2)-induced injury (p<0.01) and concluded that BCM increased the glutamate uptake capability of H(2)O(2)-injured rat astrocytes. The therapeutic benefits associated with BMSC transplantation following brain injury might be partly due to increased glutamate uptake by astrocytes.
Gui
Song-Bai
SB
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Zhang
Ya-Zhuo
YZ
Sun
Mei-Zhen
MZ
Wang
Hong-Yun
HY
He
Yue
Y
Li
Dan
D
D017667:Adipocytes / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D001391:Azo Compounds; D001854:Bone Marrow Cells / Q000502:physiology*; D002469:Cell Separation; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D005434:Flow Cytometry; D018698:Glutamic Acid / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects; D016877:Oxidants / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000502:physiology*; D018800:Thy-1 Antigens / Q000378:metabolism
D001391:Azo Compounds; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D016877:Oxidants; D018800:Thy-1 Antigens; D018698:Glutamic Acid; D006861:Hydrogen Peroxide; D000469:Alkaline Phosphatase; D017493:Leukocyte Common Antigens; C011049:oil red O