Fatty acid composition in lipid fractions lengthwise the mycelium of Mortierella isabellina and lipid production by solid state fermentation.
This paper investigates the correlation between mycelial age and fatty acid biosynthesis. The correlation was investigated by analyzing the lipid composition lengthwise the mycelium of the oleaginous fungus Mortierella isabellina, a potential producer of gamma-linolenic acid (GLA). Young mycelia were rich in polar lipids (glycolipids plus sphingolipids and phospholipids), while neutral lipid content increased in aged mycelia. In young mycelia, each polar lipid fraction contained almost 40% (w/w) polyunsaturated fatty acids (PUFAs), but this content decreased to less than 30% (w/w) in aged mycelia. On the other hand, PUFA content in neutral lipids fluctuated slightly with age. These results indicate that PUFA biosynthesis is favored in young, fast growing mycelia, while it decreases significantly in aged mycelia. This trend was also observed when we grew M. isabellina on pear pomace, an agro-industrial waste. Pear pomace cultures yielded significant amounts of lipid, which reached 12% (w/w) in dry fermented mass. The produced lipid was rich in GLA and the maximum GLA content in dry fermented mass was 2.9 mg/g.
Fakas
Stylianos
S
Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, 265 04-GR, Greece.
Makri
Anna
A
Mavromati
Maria
M
Tselepi
Maria
M
Aggelis
George
G
D000362:Agar / Q000737:chemistry; D000383:Agriculture / Q000379:methods; D005227:Fatty Acids / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D007220:Industrial Waste; D008055:Lipids / Q000737:chemistry*; D020094:Mortierella / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry; D031989:Pyrus; D011198:Solanum tuberosum; D017965:gamma-Linolenic Acid / Q000737:chemistry
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D007220:Industrial Waste; D008055:Lipids; D010743:Phospholipids; D017965:gamma-Linolenic Acid; D000362:Agar; D005947:Glucose