title: Clinical optical coherence tomography combined with multiphoton tomography of patients with skin diseases.
abstract: We report on the first clinical study based on optical coherence tomography (OCT) in combination with multiphoton tomography (MPT) and dermoscopy. 47 patients with a variety of skin diseases and disorders such as skin cancer, psoriasis, hemangioma, connective tissue diseases, pigmented lesions, and autoimmune bullous skin diseases have been investigated with (i) state-of-the-art OCT systems for dermatology including multibeam swept source OCT, (ii) the femtosecond laser multiphoton tomograph, and (iii) dermoscopes. Dermoscopy provides two-dimensional color images of the skin surface. OCT images reflect modifications of the intratissue refractive index whereas MPT is based on nonlinear excitation of endogenous fluorophores and second harmonic generation. A stack of cross-sectional OCT "wide field" images with a typical field of view of 5 x 2 mm(2) gave fast information on the depth and the volume of the lesion. Multiphoton tomography provided 0.36 x 0.36 mm(2) horizontal/diagonal optical sections within seconds of a particular region of interest with superior submicron resolution down to a tissue depth of 200 mum. The combination of OCT and MPT provides a unique powerful optical imaging modality for early detection of skin cancer and other skin diseases as well as for the evaluation of the efficiency of treatments.
authors: JenLab GmbH, Schillerstrasse 1, 07745 Jena, Germany.
authors: Karsten
authors: K
authors: König
authors: Marco
authors: M
authors: Speicher
authors: Rainer
authors: R
authors: Bückle
authors: Julia
authors: J
authors: Reckfort
authors: Gordon
authors: G
authors: McKenzie
authors: Julia
authors: J
authors: Welzel
authors: Martin J
authors: MJ
authors: Koehler
authors: Peter
authors: P
authors: Elsner
authors: Martin
authors: M
authors: Kaatz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D046169:Dermoscopy; D005260:Female; D006391:Hemangioma / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000473:pathology; D008875:Middle Aged; D010392:Pemphigus / Q000175:diagnosis / Q000473:pathology; D017785:Photons; D012867:Skin / Q000166:cytology / Q000473:pathology; D012871:Skin Diseases / Q000175:diagnosis* / Q000473:pathology; D018511:Systems Integration; D041623:Tomography, Optical Coherence