title: Altered expression of selected microRNAs in melanoma: antiproliferative and proapoptotic activity of miRNA-155.
abstract: Altered expression of microRNAs (miRNAs) has been detected in cancer, suggesting that these small non-coding RNAs can act as oncogenes or tumor suppressor genes. In the present study, we investigated the expression of miRNA-17-5p, miRNA-18a, miRNA-20a, miRNA-92a, miRNA-146a, miRNA-146b and miRNA-155 by real-time quantitative RT-PCR in a panel of melanocyte cultures and melanoma cell lines and explored the possible role of miRNA-155 in melanoma cell proliferation and survival. The analyzed miRNAs were selected on the basis of previous studies strongly supporting their involvement in cancer development and/or progression. We found that miRNA-17-5p, miRNA-18a, miRNA-20a, and miRNA-92a were overexpressed, whereas miRNA-146a, miRNA-146b and miRNA-155 were down-regulated in the majority of melanoma cell lines with respect to melanocytes. Ectopic expression of miRNA-155 significantly inhibited proliferation in 12 of 13 melanoma cell lines with reduced levels of this miRNA and induced apoptosis in 4 out of 4 cell lines analyzed. In conclusion, our data further support the finding of altered miRNA expression in melanoma cells and establish for the first time that miRNA-155 is a negative regulator of melanoma cell proliferation and survival.
authors: Laboratory of Molecular Oncology, Istituto Dermopatico dell'Immacolata-IRCCS, I-00167 Rome, Italy.
authors: Lauretta
authors: L
authors: Levati
authors: Ester
authors: E
authors: Alvino
authors: Elena
authors: E
authors: Pagani
authors: Diego
authors: D
authors: Arcelli
authors: Patrizia
authors: P
authors: Caporaso
authors: Sergio
authors: S
authors: Bondanza
authors: Gianpiero
authors: G
authors: Di Leva
authors: Manuela
authors: M
authors: Ferracin
authors: Stefano
authors: S
authors: Volinia
authors: Enzo
authors: E
authors: Bonmassar
authors: Carlo Maria
authors: CM
authors: Croce
authors: Stefania
authors: S
authors: D'Atri
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C542542:MIRN146 microRNA, human; C524942:MIRN155 microRNA, human; C505362:MIRN16 microRNA, human; C524941:MIRN17 microRNA, human; D035683:MicroRNAs