title: Ninjurin1 is expressed in myeloid cells and mediates endothelium adhesion in the brains of EAE rats.
abstract: Ninjurin1 (nerve injury-induced protein, Ninj1) is an adhesion molecule that is essential for cell-to-cell interactions. However, little is known about the function of Ninj1 in the central nervous system (CNS). To address its role in the CNS, we analyzed the expression pattern of Ninj1 in normal rats and in an experimental autoimmune encephalomyelitis (EAE) model. Ninj1 was expressed in three major compartments of brains, meninges, the choroid plexus, and parenchymal perivascular spaces. In the EAE brains, Ninj1 was strongly expressed in myeloid cells (macrophages/monocytes and neutrophils) and partially expressed in endothelial cells (ECs). Furthermore, Ninj1 enhanced adhesion between BV2 cells (murine monocyte lineage microglia) and HBMECs (human brain microvascular endothelial cells). Collectively, our findings suggest that Ninj1 may mediate the entry of myeloid cells into the CNS in normal and EAE brains, and it is a potential therapeutic target for regulating myeloid cell trafficking across the blood-brain barrier (BBB) in CNS immune processes.
authors: NeuroVascular Coordination Research Center, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Bum Ju
authors: BJ
authors: Ahn
authors: Hyo-Jong
authors: HJ
authors: Lee
authors: Min Wook
authors: MW
authors: Shin
authors: Jeong-Hyun
authors: JH
authors: Choi
authors: Joo-Won
authors: JW
authors: Jeong
authors: Kyu-Won
authors: KW
authors: Kim
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis*; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D004727:Endothelium / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D051381:Rats; D011917:Rats, Inbred Lew; D015854:Up-Regulation
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D009414:Nerve Growth Factors; C101196:Ninj1 protein, rat