title: Antibacterial effects and dissolution behavior of six bioactive glasses.
abstract: Dissolution behavior of six bioactive glasses was correlated with the antibacterial effects of the same glasses against sixteen clinically important bacterial species. Powdered glasses (<45 microm) were immersed in simulated body fluid (SBF) for 48 h. The pH in the solution inside the glass powder was measured in situ with a microelectrode. After 2, 4, 27, and 48 h, the pH and concentration of ions after removing the particles and mixing the SBF were measured with a normal glass pH electrode and ICP-OES. The bacteria were cultured in broth with the glass powder for up to 4 days, after which the viability of the bacteria was determined. The antibacterial effect of the glasses increased with increasing pH and concentration of alkali ions and thus with increased dissolution tendency of the glasses, but it also depended on the bacterium type. The changes in the concentrations of Si, Ca, Mg, P, and B ions in SBF did not show statistically significant influence on the antibacterial property. Bioactive glasses showed strong antibacterial effects for a wide selection of aerobic bacteria at a high sample concentration (100 mg/mL). The antibacterial effects increased with glass concentration and a concentration of 50 mg/mL (SA/V 185 cm(-1)) was required to generate the bactericidal effects. Understanding the dissolution mechanisms of bioactive glasses is essential when assessing their antibacterial effects.
authors: Process Chemistry Centre, Abo Akademi University, Piispankatu 8, Turku FI-20500, Finland.
authors: Di
authors: D
authors: Zhang
authors: Outi
authors: O
authors: Leppäranta
authors: Eveliina
authors: E
authors: Munukka
authors: Heimo
authors: H
authors: Ylänen
authors: Matti K
authors: MK
authors: Viljanen
authors: Erkki
authors: E
authors: Eerola
authors: Mikko
authors: M
authors: Hupa
authors: Leena
authors: L
authors: Hupa
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001420:Bacteria, Aerobic / Q000187:drug effects* / Q000502:physiology; D001672:Biocompatible Materials / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials