A NAC transcription factor gene of Chickpea (Cicer arietinum), CarNAC3, is involved in drought stress response and various developmental processes.
NAC transcription factors have been found to play important roles in plant development and responses to environmental stresses. Based on two cDNA libraries constructed from the PEG-treated and -nontreated seedling leaves of chickpea, a NAC gene, CarNAC3, was isolated and characterized. The results indicated that CarNAC3 contained 285 amino acids and had a conserved NAC domain. It was localized in the nucleus and possessed trans-activation activity in the C-terminus. Phylogenetic analysis showed that CarNAC3 belonged to the NAP (NAC-like, activated by APETALA3/PISTILLATA) subgroup of the NAC protein family. CarNAC3 exhibited organ-specific expression and its induction was strongly dependent on leaf age. CarNAC3 showed differential expression patterns during seed development and germination, and could be significantly induced by drought stress, abscisic acid (ABA), ethephon (Et) and indole-3-acetic acid (IAA), but was inhibited by N-6-benzyl-adenine (6-BA). Our data suggest that CarNAC3 may be a transcriptional activator involved in drought stress response and various developmental processes.
Peng
Hui
H
State Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing 210095, China.
Cheng
Hui-Ying
HY
Chen
Chen
C
Yu
Xin-Wang
XW
Yang
Jia-Ni
JN
Gao
Wen-Rui
WR
Shi
Qing-Hua
QH
Zhang
Hua
H
Li
Jian-Gui
JG
Ma
Hao
H
D000595:Amino Acid Sequence; D029863:Cicer / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D017124:Conserved Sequence; D055864:Droughts; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D016415:Sequence Alignment; D013312:Stress, Physiological; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014867:Water / Q000378:metabolism*
D010937:Plant Growth Regulators; D010940:Plant Proteins; D015534:Trans-Activators; D014867:Water