title: An insight into the transcriptome and proteome of the salivary gland of the stable fly, Stomoxys calcitrans.
abstract: Adult stable flies are blood feeders, a nuisance, and mechanical vectors of veterinary diseases. To enable efficient feeding, blood sucking insects have evolved a sophisticated array of salivary compounds to disarm their host's hemostasis and inflammatory reaction. While the sialomes of several blood sucking Nematocera flies have been described, no thorough description has been made so far of any Brachycera, except for a detailed proteome analysis of a tabanid (Xu et al., 2008). In this work we provide an insight into the sialome of the muscid Stomoxys calcitrans, revealing a complex mixture of serine proteases, endonucleases, Kazal-containing peptides, anti-thrombins, antigen 5 related proteins, antimicrobial peptides, and the usual finding of mysterious secreted peptides that have no known partners, and may reflect the very fast evolution of salivary proteins due to the vertebrate host immune pressure. Supplemental Tables S1 and S2 can be downloaded from http://exon.niaid.nih.gov/transcriptome/S_calcitrans/T1/Sc-tb1-web.xls and http://exon.niaid.nih.gov/transcriptome/S_calcitrans/T2/Sc-tb2-web.xls.
authors: Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA.
authors: Xuyong
authors: X
authors: Wang
authors: José M C
authors: JM
authors: Ribeiro
authors: Alberto B
authors: AB
authors: Broce
authors: Melinda J
authors: MJ
authors: Wilkerson
authors: Michael R
authors: MR
authors: Kanost
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009117:Muscidae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D019476:Insect Proteins; D020543:Proteome