title: Digital holographic microscopy for quantitative cell dynamic evaluation during laser microsurgery.
abstract: Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system for evaluation of cellular/ sub-cellular dynamic changes during laser micro-dissection. The proposed method takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system will permit quantitative evaluation of the damage and/or the repair of the cell or cell organelles in real time.
authors: Beckman Laser Institute, University of California, Irvine, Irvine, CA 92617, USA. yulingfeng@gmail.com
authors: Lingfeng
authors: L
authors: Yu
authors: Samarendra
authors: S
authors: Mohanty
authors: Jun
authors: J
authors: Zhang
authors: Suzanne
authors: S
authors: Genc
authors: Myung K
authors: MK
authors: Kim
authors: Michael W
authors: MW
authors: Berns
authors: Zhongping
authors: Z
authors: Chen
mesh_terms: D000465:Algorithms; D000818:Animals; D004867:Equipment Design; D004912:Erythrocytes / Q000473:pathology; D006696:Holography / Q000379:methods; D006801:Humans; D007368:Interferometry / Q000379:methods*; D007668:Kidney / Q000473:pathology; D007834:Lasers; D008866:Microsurgery / Q000295:instrumentation* / Q000379:methods; D052898:Optical Tweezers; D010363:Pattern Recognition, Automated / Q000379:methods; D048328:Potoroidae; D012984:Software