Purification of drugs from biological fluids by counter-current chromatography.
Experiments were performed to demonstrate the potential of counter-current chromatography (CCC) for the isolation of drugs and their metabolites from biological matrices relevant to the metabolism studies of pharmaceutical research. Examples of typical drugs are spiked into biological media ex vivo to provide test samples for analysis. A mass spectrometer hyphenated to a CCC allows for the detection of small molecule drugs within the matrix through selected ion monitoring, and fraction collection can provide material for further structural elucidation by NMR.
Hochlowski
Jill E
JE
Abbott Laboratories, Abbott Park, IL 60064, USA. hochlowskij@abbott.com
Pan
Jeffrey Y
JY
Searle
Philip A
PA
Buck
Wayne R
WR
Spanton
Stephen G
SG
D053000:Analytic Sample Preparation Methods; D000818:Animals; D001646:Bile / Q000737:chemistry; D001774:Blood Chemical Analysis; D001826:Body Fluids / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D004285:Dogs; D006801:Humans; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000097:blood / Q000302:isolation & purification*; D012997:Solvents
D004364:Pharmaceutical Preparations; D012997:Solvents