Presenilin enhancer-2 (PSENEN), a component of the gamma-secretase complex, is involved in adipocyte differentiation.
This study was conducted to identify genes expressed during adipocyte differentiation of bovine intramuscular fibroblast-like cells using differential display reverse-transcriptase polymerase chain reaction. The presenilin enhancer-2 (PSENEN) gene was found to be down-regulated during adipocyte differentiation of bovine intramuscular fibroblast-like cells. The ectopic expression of bovine PSENEN in 3T3-L1 reduced adipogenesis and the inhibition of endogenous PSENEN by siRNA induced adipogenesis on d 4 of adipocyte differentiation of 3T3-L1 cells. Interestingly, the expression of gamma-secretase complex gene-related Notch signaling was decreased at d 2 and d 4 during adipocyte differentiation. In addition, expression of the Notch-signaling genes (Notch-1, Hes-1, Pref-1, adipsin) was regulated during adipocyte differentiation by regulation of PSENEN expression. These results suggest that PSENEN plays an important role in adipocyte differentiation and that Notch signaling is involved in adipogenesis.
Lee
S M
SM
Department of Animal Science, College of Agriculture and Life Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea.
Jeong
Y H
YH
Kim
H M
HM
Park
H Y
HY
Yoon
D
D
Kim
D H
DH
Saeki
S
S
Moon
S J
SJ
Kang
M J
MJ
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D008969:Molecular Sequence Data; D053766:Presenilin-2 / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment
D053766:Presenilin-2