title: Refinement of protein structures into low-resolution density maps using rosetta.
abstract: We describe a method based on Rosetta structure refinement for generating high-resolution, all-atom protein models from electron cryomicroscopy density maps. A local measure of the fit of a model to the density is used to directly guide structure refinement and to identify regions incompatible with the density that are then targeted for extensive rebuilding. Over a range of test cases using both simulated and experimentally generated data, the method consistently increases the accuracy of starting models generated either by comparative modeling or by hand-tracing the density. The method can achieve near-atomic resolution starting from density maps at 4-6 A resolution.
authors: Department of Biochemistry, University of Washington, Seattle, 98195, USA. dimaio@u.washington.edu
authors: Frank
authors: F
authors: DiMaio
authors: Michael D
authors: MD
authors: Tyka
authors: Matthew L
authors: ML
authors: Baker
authors: Wah
authors: W
authors: Chiu
authors: David
authors: D
authors: Baker
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D011506:Proteins