title: Clinical manifestations, antibiotic susceptibility and molecular analysis of Mycobacterium kansasii isolates from a university hospital in Taiwan.
abstract: OBJECTIVE
  Mycobacterium kansasii causes a variety of infections. Although previous reports on the prognosis of antimicrobial therapy have been mostly satisfactory, problems involving treatment failure or relapse have been encountered. The purpose of this study was to establish a relationship between the clinical treatment outcomes of M. kansasii infections and bacterial drug susceptibility, and their clonality.
  
  
  METHODS
  A total of 37 M. kansasii clinical isolates and clinical information on 34 patients were retrospectively collected in a tertiary medical centre in Taiwan. Bacterial drug susceptibility was determined by the microdilution method. The phylogenetic relationship was analysed by PFGE analysis.
  
  
  RESULTS
  Results of PFGE typing revealed a major cluster (cluster I) and eight other divergent patterns. Two/three strains leading to treatment failure were also multidrug resistant and belonged to cluster I.
  
  
  CONCLUSIONS
  A relationship between high drug resistance and genetic relatedness of some M. kansasii strains was established. This was associated with clinical treatment failure.
authors: Division of Infectious Diseases, Department of Internal Medicine, Chang Gung Memorial Hospital, Gueishan Shiang 33305, Taoyuan, Taiwan.
authors: Ting-Shu
authors: TS
authors: Wu
authors: Hsieh-Shong
authors: HS
authors: Leu
authors: Cheng-Hsun
authors: CH
authors: Chiu
authors: Ming-Hsun
authors: MH
authors: Lee
authors: Ping-Cherng
authors: PC
authors: Chiang
authors: Tsu-Lan
authors: TL
authors: Wu
authors: Ju-Hsin
authors: JH
authors: Chia
authors: Lin-Hui
authors: LH
authors: Su
authors: An-Jing
authors: AJ
authors: Kuo
authors: Hsin-Chih
authors: HC
authors: Lai
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006785:Hospitals, University; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology*; D019909:Mycobacterium kansasii / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D012189:Retrospective Studies; D013624:Taiwan; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial