title: Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5.
abstract: Haemophilus parasuis is the aetiological agent of Glässer's disease, which is responsible for cases of fibrinous polyserositis, polyarthritis and meningitis in young pigs. To develop more effective vaccines, an immunoproteome-based approach was used to analyze the outer membrane proteins of H. parasuis serovar 5. A total of 15 proteins with high immunogenicity were identified and all were showed to be immunogens for the first time in H. parasuis. Further analyses of 8 selected proteins revealed that (1) significantly higher level of serum antibodies against 6 proteins was detected with convalescent sera and immunized sera; (2) antisera against 5 of the selected proteins could effectively inhibit H. parasuis growth in mouse blood; and (3) 4 proteins could induce protective response of the vaccinated mice against H. parasuis. The results suggest these 4 proteins (PalA, Omp2, D15 and HPS_06257) have strong potential to be vaccine candidates.
authors: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Mingguang
authors: M
authors: Zhou
authors: Yi
authors: Y
authors: Guo
authors: Jianping
authors: J
authors: Zhao
authors: Qiaoyun
authors: Q
authors: Hu
authors: Yong
authors: Y
authors: Hu
authors: Anding
authors: A
authors: Zhang
authors: Huanchun
authors: H
authors: Chen
authors: Meilin
authors: M
authors: Jin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control*; D044137:Haemophilus parasuis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines