Actinopolymorpha alba sp. nov., isolated from a rhizosphere soil.
A Gram-positive, milk-white coloured, aerobic strain, YIM 48868T, was isolated from the rhizosphere soil of Maytenus hookeri Loes in Xishuangbanna, China. 16S rRNA gene sequence similarity studies showed that strain YIM 48868T was a member of the genus Actinopolymorpha, showing 96.8% sequence similarity to Actinopolymorpha singaporensis IM 7744T and 97.0% similarity to Actinopolymorpha rutila YIM 45725T. Chemotaxonomic data (peptidoglycan type I, ll-diaminopimelic acid; sugar pattern C, glucose, rhamnose and ribose; polar lipids PI, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannosides and phosphatidylinositol) were characteristic of the genus Actinopolymorpha. A phylogenetic tree based on 16S rRNA gene sequences showed that strain YIM 48868T formed a distinct phylogenetic lineage within the genus Actinopolymorpha. Strain YIM 48868T could be differentiated from recognized species by means of phenotypic properties and the predominant menaquinones [MK-9(H6), MK-9(H8), MK-10(H6), MK-10(H8)]. The DNA G+C content was 66.6 mol%. The DNA-DNA relatedness values between strain YIM 48868T and the type strains of A. singaporensis and A. rutila were 48.7% and 53.1%, respectively. These data, in combination with phenotypic and chemotaxonomic data, demonstrate that strain YIM 48868T represents a novel species in the genus Actinopolymorpha, for which the name Actinopolymorpha alba sp. nov. is proposed. The type strain is YIM 48868T (=CCTCC AA 208030T=DSM 45243T).
Cao
Yan-Ru
YR
Yunnan Institute of Microbiology, The National Engineering Center for Research of Microbial Pharmaceuticals, Yunnan University, Kunming, Yunnan 650091, PR China.
Jiang
Yi
Y
Wu
Jin-Yuan
JY
Xu
Li-Hua
LH
Jiang
Cheng-Lin
CL
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D029755:Maytenus / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2