pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle.
A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.
Yoshii
Noriyo
N
Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan.
Ogura
Yoshitoshi
Y
Hayashi
Tetsuya
T
Ajiro
Takashi
T
Sameshima
Toshiya
T
Nakazawa
Muneo
M
Kusumoto
Masahiro
M
Iwata
Taketoshi
T
Akiba
Masato
M
D000818:Animals; D002417:Cattle; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
D004269:DNA, Bacterial