title: Millisecond analysis of double stranded DNA with fluorescent intercalator by micro-thermocontrol-device.
abstract: Study of interaction between DNA and intercalator at molecular level is important to understand the mechanisms of DNA replication and repair. A micro-fabricated local heating thermodevice was adapted to perform denaturation experiments of DNA with fluorescent intercalator on millisecond time scale. Response time of complete unzipping of double stranded DNA, 16 microm in length, was measured to be around 5 min by commercial thermocycler. Response time of quenching of double stranded DNA with fluorescent intercalator SYBR Green was measured to be 10 ms. Thus, quenching properties owing to strand unzipping and denaturation at base pair level were distinguished. This method has provided easy access to measure this parameter and may be a powerful methodology in analyzing biomolecules on millisecond time scale.
authors: Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. arata@sanken.osaka-u.ac.jp
authors: Hideyuki F
authors: HF
authors: Arata
authors: Frederic
authors: F
authors: Gillot
authors: Dominique
authors: D
authors: Collard
authors: Hiroyuki
authors: H
authors: Fujita
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D007364:Intercalating Agents / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D013696:Temperature; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D007364:Intercalating Agents; D004247:DNA