Genome profiling of chondrosarcoma using oligonucleotide array-based comparative genomic hybridization.
Chondrosarcomas of the bone are malignant hyaline cartilage-forming tumors with an annual incidence rate of 3.6% of all primary bone malignancies in the United States. Specimens of 25 chondrosarcomas (10 grade I, 9 grade II, 1 grade III, and 5 dedifferentiated) from 23 patients were collected from the Department of Pathology at the University Hospital at UMDNJ-New Jersey Medical School from 1996 to 2007. Array-based comparative genomic hybridization (array-CGH) studies were performed on frozen tumor specimens. Recurrent deletions observed in at least in six tumors were 5q13.2, 5q14.2 approximately q21.3, 6q12 approximately q13, 6q16 approximately q25.3, 9p24.2 approximately q12, and 9p21.3. There was a statistically significant association between high-grade tumor (grade III and dedifferentiated) and the recurrent genetic deletions at 5q14.2 approximately q21.3, 6q16 approximately q25.3, 9p24.2 approximately q12, and 9p21.3. There is consistency between increased levels of aneuploidy and the progression of chondrosarcoma from lower to higher grades.
Hameed
Meera
M
Institute of Genomic Medicine, UMDNJ-New Jersey Medical School, 225 Warren Street, Newark, NJ 07103, USA.
Ulger
Celal
C
Yasar
Duygu
D
Limaye
Neha
N
Kurvathi
Rohini
R
Streck
Deanna
D
Benevenia
Joseph
J
Patterson
Francis
F
Dermody
James J
JJ
Toruner
Gokce A
GA
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002813:Chondrosarcoma / Q000235:genetics*; D002872:Chromosome Deletion; D055028:Comparative Genomic Hybridization; D005260:Female; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis