Omentopexy enhances graft function in myocardial cell sheet transplantation.
Myocardial cell sheets (MCS) are a potentially valuable tool for tissue engineering aimed at heart regeneration. Several methods have recently been established for the fabrication of MCS. However, the lack of a sufficient blood supply has inhibited functional recovery of the MCS. To address this challenge, we combined MCS transplantation with omentopexy (OP), which utilizes omental tissue as a surgical flap. Rats were divided into five groups: sham, myocardial infarction (MI), MCS transplantation, OP, and MCS+OP. Histologic analysis revealed that MCS+OP drastically reversed MI-induced cardiac remodeling. Echocardiography revealed that MCS increased cardiac function, while OP had a synergistic beneficial effect with MCS transplantation. Immunofluorescence imaging showed that OP increased the survival of transplanted cardiomyocytes, and increased the blood supply through enhancement of angiogenesis and migration of small arteries into the MCS. Taken together, we concluded that OP is a promising strategy for the enhancement of graft function in MCS transplantation.
Suzuki
Ryo
R
Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan.
Hattori
Fumiyuki
F
Itabashi
Yuji
Y
Yoshioka
Masatoyo
M
Yuasa
Shinsuke
S
Manabe-Kawaguchi
Haruko
H
Murata
Mitsushige
M
Makino
Shinji
S
Kokaji
Kiyokazu
K
Yozu
Ryohei
R
Fukuda
Keiichi
K
D000818:Animals; D004452:Echocardiography; D006085:Graft Survival; D009203:Myocardial Infarction / Q000601:surgery; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000000981:diagnostic imaging / Q000502:physiology* / Q000637:transplantation*; D009852:Omentum; D051381:Rats; D011923:Rats, Nude; D055647:Rats, Transgenic; D012038:Regeneration