title: Biotechnological production of enantiopure epoxides by enzymatic kinetic resolution.
abstract: Enantiopure epoxides are high value-added synthons for the production of pharmaceuticals, agrochemicals, as well as versatile fine chemicals and have broad scope of market demand for their applications. A major challenge in conventional organic synthesis is to generate such compounds in high enantiopurity with reasonable yield. Among possible chemical and biological technologies for enantiopure epoxide preparation, enzymatic kinetic resolution has been paid much attention with respect to its high enantioselectivity. Epoxide hydrolase (EH) has shown promising characteristics for the preparation of enantiopure epoxides and vicinal diols during enantioselective hydrolysis of racemic epoxides. EH is readily available from microbial resources thus it is being employed for biohydrolysis of a variety of epoxides. Recent technical progress in EH-catalyzed enantioselective hydrolysis is summarized in terms of exploration of novel EH, its functional improvement, high throughput assay, and preparative scale resolution process.
authors: Institute of Chemical and Engineering Sciences, A*STAR (Agency for Science, Technology and Research), Jurong Island, Singapore. choi_won_jae@ices.a-star.edu.sg
authors: Won Jae
authors: WJ
authors: Choi
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D002384:Catalysis; D002626:Chemistry, Pharmaceutical; D004851:Epoxide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry / Q000378:metabolism*; D007218:Industrial Microbiology; D007700:Kinetics; D013237:Stereoisomerism
chemical_list: D004852:Epoxy Compounds; D004851:Epoxide Hydrolases