Protein hydrolysates from beta-conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro.
Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory effect of SPH produced by alcalase on LPS-induced macrophage RAW 264.7 cell line was also investigated. SAH (100 microM) derived from soybean enriched in beta-conglycinin (BC) (up to 47% total protein) decreased lipid accumulation (33-37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 microM) inhibited lipid accumulation to a lesser extent (8-14% inhibition) through inhibition of LPL gene expression. SAH (5 microM) decreased the production of nitric oxide (NO) (18-35%) and prostaglandin E(2) (PGE(2)) (47-71%) and the expression of inducible nitric oxide synthase (iNOS) (31-53%) and cycloxygenase-2 (COX-2) (30-52%). This is the first investigation showing that soy hydrolysates inhibit LPS-induced iNOS/NO and COX-2/PGE(2 )pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food.
Martinez-Villaluenga
Cristina
C
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, IL, USA.
Dia
Vermont P
VP
Berhow
Mark
M
Bringe
Neal A
NA
Gonzalez de Mejia
Elvira
E
D041721:3T3-L1 Cells; D000818:Animals; D052179:Antigens, Plant / Q000494:pharmacology*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis; D064429:Fatty Acid Synthases / Q000235:genetics; D005838:Genotype; D005916:Globulins / Q000494:pharmacology*; D007249:Inflammation / Q000517:prevention & control*; D050356:Lipid Metabolism / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000235:genetics; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D010940:Plant Proteins / Q000032:analysis; D055314:Seed Storage Proteins / Q000494:pharmacology*; D030262:Soybean Proteins / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*
D052179:Antigens, Plant; D052246:Cyclooxygenase 2 Inhibitors; D005916:Globulins; D010940:Plant Proteins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase; D015232:Dinoprostone