title: Involvement of GATA transcription factors in the regulation of endogenous bovine interferon-tau gene transcription.
abstract: Expression of interferon-tau (IFNT), necessary for pregnancy establishment in ruminant ungulates, is regulated in a temporal and spatial manner. However, molecular mechanisms by which IFNT gene transcription is regulated in this manner have not been firmly established. In this study, DNA microarray/RT-PCR analysis between bovine trophoblast CT-1 and Mardin-Darby bovine kidney (MDBK) cells was initially performed, finding that transcription factors GATA2, GATA3, and GATA6 mRNAs were specific to CT-1 cells. These mRNAs were also found in Days 17, 20, and 22 (Day 0 = day of estrus) bovine conceptuses. In examining other bovine cell lines, ovary cumulus granulosa (oCG) and ear fibroblast (EF) cells, GATA2 and GATA3, but not GATA6, were found specific to the bovine trophoblast cells. In transient transfection analyses using the upstream region (-631 to +59 bp) of bovine IFNT gene (bIFNT, IFN-tau-c1), over-expression of GATA2/GATA3 did not affect the transcription of bIFNT-reporter construct in human choriocarcinoma JEG3 cells. Transfection of GATA2, GATA3, ETS2, and/or CDX2, however, was effective in the up-regulation of the bIFNT construct transfected into bovine oCG and EF cells. One Point mutation studies revealed that among six potential GATA binding sites located on the upstream region of the bIFNT gene, the one next to ETS2 site exhibited reduced luciferase activity. In CT-1 cells, endogenous bIFNT gene transcription was up-regulated by over-expression of GATA2 or GATA3, but down-regulated by siRNA specific to GATA2 mRNA. These data suggest that GATA2/3 is involved in trophoblast-specific regulation of bIFNT gene transcription.
authors: Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo, Tokyo 113-8657, Japan.
authors: Hanako
authors: H
authors: Bai
authors: Toshihiro
authors: T
authors: Sakurai
authors: Min-Su
authors: MS
authors: Kim
authors: Yoshikage
authors: Y
authors: Muroi
authors: Atsushi
authors: A
authors: Ideta
authors: Yoshito
authors: Y
authors: Aoyagi
authors: Hiromi
authors: H
authors: Nakajima
authors: Masashi
authors: M
authors: Takahashi
authors: Kentaro
authors: K
authors: Nagaoka
authors: Kazuhiko
authors: K
authors: Imakawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D050980:GATA Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D006801:Humans; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017354:Point Mutation; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D050787:Proto-Oncogene Protein c-ets-2 / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism
chemical_list: D050980:GATA Transcription Factors; D007370:Interferon Type I; D011257:Pregnancy Proteins; D020033:Protein Isoforms; D050787:Proto-Oncogene Protein c-ets-2; C020947:interferon tau