title: Serodiagnostic contributions of antibody titers against mycobacterial lipid antigens in Mycobacterium avium complex pulmonary disease.
abstract: BACKGROUND
  Although the incidence of pulmonary tuberculosis is decreasing, the number of immunocompetent patients with Mycobacterium avium complex (MAC) pulmonary disease is steadily increasing. Therefore, albeit not contagious, MAC pulmonary disease needs to be diagnosed rapidly and accurately. We examined the serodiagnostic contributions of serum immunoglobulin G antibody titers against the species-specific and -common mycobacterial lipid antigens in the diagnosis of MAC pulmonary disease.
  
  
  METHODS
  Serum samples were obtained from 65 patients with MAC pulmonary disease, 15 patients with suspected MAC disease, 25 patients with pulmonary tuberculosis, 10 patients with Mycobacterium kansasii disease, and 100 healthy volunteers (control subjects). We measured the serum immunoglobulin G antibody titers against trehalose monomycolate (TMM-M) and apolar-glycopeptidolipid (GPL), lipid antigens extracted from MAC.
  
  
  RESULTS
  In patients with MAC pulmonary disease, the antibody titers against TMM-M and apolar-GPL were significantly higher than those in the other patient groups or in the control subjects. By receiver operator characteristic curve analysis, an optical density of 0.27, corresponding to the optimal cutoff antibody titer against TMM-M, was associated with a sensitivity of 89.2% and a specificity of 97.0%, and an optical density of 0.33, corresponding to the optimal cutoff antibody titer against apolar-GPL, was associated with a sensitivity of 89.2% and a specificity of 94.0%.
  
  
  CONCLUSIONS
  Measurements of antibody titers against TMM-M and apolar-GPL would be useful in the diagnosis of MAC pulmonary disease and in the differential diagnosis of mycobacterial pulmonary disease.
authors: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan.
authors: Tomoyasu
authors: T
authors: Nishimura
authors: Naoki
authors: N
authors: Hasegawa
authors: Yukiko
authors: Y
authors: Fujita
authors: Ikuya
authors: I
authors: Yano
authors: Akitoshi
authors: A
authors: Ishizaka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D003311:Cord Factors / Q000276:immunology; D005260:Female; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008055:Lipids / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D015269:Mycobacterium avium Complex / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003311:Cord Factors; D006017:Glycolipids; D007074:Immunoglobulin G; D008055:Lipids; C021099:trehalose monomycolate