Kineococcus rhizosphaerae sp. nov., isolated from rhizosphere soil.
An orange-coloured actinomycete, designated strain RP-B16T, was isolated from a rhizosphere soil. Cells of strain RP-B16T were strictly aerobic, Gram-positive-staining, oxidase-negative, catalase-positive, motile cocci. A phylogenetic tree, based on 16S rRNA gene sequences, showed that members of the genus Kineococcus were divided into two independent clusters and that strain RP-B16T formed a coherent clade with the type strains of Kineococcus aurantiacus (99.5% sequence similarity) and Kineococcus radiotolerans (99.1%). Levels of 16S rRNA gene sequence similarity between strain RP-B16T and Kineococcus marinus KST3-3T and 'Kineococcus tegulae' T2A-S27 were lower than 97.0%. Chemotaxonomic characteristics were typical of the genus Kineococcus in having meso-diaminopimelic acid as the diagnostic diamino acid, MK-9(H2) as the predominant menaquinone, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids, anteiso-C15:0 as the predominant fatty acid and a DNA G+C content of 73.8 mol%. Strain RP-B16T showed levels of DNA-DNA relatedness of 34.6-37.3% to Kineococcus aurantiacus DSM 7487T and 31.2-32.7% to Kineococcus radiotolerans DSM 14245T. On the basis of phenotypic features and DNA-DNA hybridization data, strain RP-B16T is considered to represent a novel species of the genus Kineococcus, for which the name Kineococcus rhizosphaerae sp. nov. is proposed. The type strain is RP-B16T (=KCTC 19366T=DSM 19711T).
Lee
Soon Dong
SD
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea. sdlee@cheju.ac.kr
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; D010088:Oxidoreductases; D002374:Catalase