title: [Contamination with genetically modified maize MON863 of processed foods on the market].
abstract: Genetically modified maize MON863 (MON863), which has passed a safety examination in Japan, is commercially cultivated in the United States as a food and a resource for fuel. Maize is an anemophilous flower, which easily hybridizes. However, an official method for quantifying the content of MON863 has not been provided yet in Japan. We here examined MON863 contamination in maize-processed foods that had no labeling indicating of the use of genetically modified maize.From March 2006 to July 2008, we purchased 20 frozen maize products, 8 maize powder products, 7 canned maize products and 4 other maize processed foods. Three primer pairs named MON 863 primer, MON863-1, and M3/M4 for MON863-specific integrated cassette were used for qualitative polymerase chain reaction (PCR). A primer pair "SSIIb-3" for starch synthase gene was used to confirm the quality of extracted DNA. The starch synthase gene was detected in all samples. In qualitative tests, the MON863-specific fragments were detected in 7 (18%) maize powder products out of the 39 processed foods with all the three primer pairs.We concluded that various maize processed foods on the market were contaminated with MON863. It is important to accumulate further information on MON863 contamination in maize-processed foods that have no label indication of the use of genetically modified maize.
authors: Sapporo City Institute of Public Health, Shiroishi-ku, Sapporo 0038505, Japan.
authors: Yoko
authors: Y
authors: Ohgiya
authors: Masaaki
authors: M
authors: Sakai
authors: Taeko
authors: T
authors: Miyashita
authors: Koichi
authors: K
authors: Yano
mesh_terms: D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling; D030841:Food, Genetically Modified; D016133:Polymerase Chain Reaction; D003313:Zea mays / Q000235:genetics