Effects of diode laser therapy on the acellular dermal matrix.
Acellular dermal matrix (ADM) was subcutaneously implanted into calvarian skin of male Wistar rats (n = 40). Low-level laser (lambda 685 nm, 4 J/cm(2)) was locally applied in experimental group (n = 20) above the skin flap. Grafts were harvested at 1, 3, 7 and 14 days after surgery and underwent histological analyses. In treated animals, the extent of edema and the number of inflammatory cells were reduced (P < 0.05). The amount of collagen in graft treated with low-level laser were significantly higher than those of controls (P < 0.05) and were statistically more prominent on the 14th day after surgery. The mean count of fibroblasts was significantly higher in the low-laser therapy group within the 3rd day, showing a marked influx of fibroblasts into area. In conclusion, wound healing of the ADM appear to be positively affected by laser therapy.
Soares
Lívia Prates
LP
Department of Oralmaxillofacial Surgery, PUCRS School of Dentistry, Porto Alegre, Rio Grande do Sul, Brazil. liviaps@ibest.com.br
de Oliveira
Marília Gerhardt
MG
de Almeida Reis
Sílvia Regina
SR
D000818:Animals; D003094:Collagen / Q000627:therapeutic use*; D005347:Fibroblasts / Q000166:cytology; D006085:Graft Survival; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation; D017768:Skin, Artificial; D014183:Transplantation, Heterologous; D014945:Wound Healing
C440568:Alloderm; D003094:Collagen