Targeted optical injection of gold nanoparticles into single mammalian cells.
We present an all optical technique for the targeted delivery of single 100 nm diameter gold nanoparticles into a specified region of the interior of an individual mammalian cell through a combination of optical tweezing and optical injection. The internalisation of the nanoparticle is verified by confocal laser scanning microscopy and confocal laser scanning reflectance microscopy. This represents the first time that nano sized particles have been tweezed and optically injected into mammalian cells using only light, and provides a novel methodology for internalising nanosphere based biosensors within specific intracellular regions of a mammalian cell.
McDougall
Craig
C
SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK. cm70@st-andrews.ac.uk
Stevenson
David J
DJ
Brown
Christian T A
CT
Gunn-Moore
Frank
F
Dholakia
Kishan
K
D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002477:Cells / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D007267:Injections; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D018613:Microscopy, Confocal; D055620:Optical Phenomena; D052898:Optical Tweezers; D010316:Particle Size; D016062:Porosity
D006046:Gold