[Expression levels of Cdc2 and Cdc25A mRNA in cattle, yak, and cattle-yak testis].
The infertility of cattle-yak, which is the hybrid offspring of cattle and yak, is a difficult problem in crossbreeding and improvement of yak. Cdc2 and Cdc25A are the key genes of meiosis. The decline of their expression levels will cause the spermatogenesis failure and lead to infertility. Therefore, this study was conducted to study the relationship between the infertility of cattle-yak and the expression levels of Cdc2/Cdc25A genes. The expression profiles were obtained by RT-PCR. Cdc2 and Cdc25A genes were widely expressed in many tissues, which confirmed their important role in cell division and the progression of cell cycle. Real-time quantitative PCR analysis indicated that the expression levels of Cdc2 and Cdc25A in cattle and yak testis were higher than those in cattle-yak (P<0.05). Therefore, low expression levels of Cdc2 and Cdc25A genes may have a relationship with the infertility of cattle-yak.
Dong
Li-Yan
LY
Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjng 210095, China. dongliyan2008@126.com
Li
Qi-Fa
QF
Qu
Xu-Guang
XG
Li
Yin-Xia
YX
Li
Xin-Fu
XF
Hu
Hong-Tao
HT
Xie
Zhuang
Z
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D015870:Gene Expression; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D020687:cdc25 Phosphatases / Q000235:genetics* / Q000378:metabolism
D000199:Actins; D012333:RNA, Messenger; D016203:CDC2 Protein Kinase; D020687:cdc25 Phosphatases