title: Fatty acid composition in lipid fractions lengthwise the mycelium of Mortierella isabellina and lipid production by solid state fermentation.
abstract: This paper investigates the correlation between mycelial age and fatty acid biosynthesis. The correlation was investigated by analyzing the lipid composition lengthwise the mycelium of the oleaginous fungus Mortierella isabellina, a potential producer of gamma-linolenic acid (GLA). Young mycelia were rich in polar lipids (glycolipids plus sphingolipids and phospholipids), while neutral lipid content increased in aged mycelia. In young mycelia, each polar lipid fraction contained almost 40% (w/w) polyunsaturated fatty acids (PUFAs), but this content decreased to less than 30% (w/w) in aged mycelia. On the other hand, PUFA content in neutral lipids fluctuated slightly with age. These results indicate that PUFA biosynthesis is favored in young, fast growing mycelia, while it decreases significantly in aged mycelia. This trend was also observed when we grew M. isabellina on pear pomace, an agro-industrial waste. Pear pomace cultures yielded significant amounts of lipid, which reached 12% (w/w) in dry fermented mass. The produced lipid was rich in GLA and the maximum GLA content in dry fermented mass was 2.9 mg/g.
authors: Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, 265 04-GR, Greece.
authors: Stylianos
authors: S
authors: Fakas
authors: Anna
authors: A
authors: Makri
authors: Maria
authors: M
authors: Mavromati
authors: Maria
authors: M
authors: Tselepi
authors: George
authors: G
authors: Aggelis
mesh_terms: D000362:Agar / Q000737:chemistry; D000383:Agriculture / Q000379:methods; D005227:Fatty Acids / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D007220:Industrial Waste; D008055:Lipids / Q000737:chemistry*; D020094:Mortierella / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry; D031989:Pyrus; D011198:Solanum tuberosum; D017965:gamma-Linolenic Acid / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D007220:Industrial Waste; D008055:Lipids; D010743:Phospholipids; D017965:gamma-Linolenic Acid; D000362:Agar; D005947:Glucose