title: [Anti-inflammatory effects and quantitative study of the combinations of active ingredients of Painong powder in mice].
abstract: OBJECTIVE
  To study the anti-inflammatory effects of the combinations of active components of Painong powder, a compound traditional Chinese herbal medicine, and the quantitative analysis of their interactions.
  
  
  METHODS
  The mouse model of acute inflammation with increase of capillary permeability was induced by intraperitoneal injection of acetic acid. An orthogonal design with 2 levels (used and unused) was applied to assign the combinations groups of active ingredients including naringin and neohesperidin, peoniflorin, and platycodin. Aspirin and normal saline were administered as control. The pharmacodynamic interactions were analyzed by the optical density (OD) of infiltrated Evans blue.
  
  
  RESULTS
  The different combinations of active ingredients showed anti-inflammatory effect with different degree, and the predicted values of OD varied from 0.115 to 0.170. The maximum anti-inflammatory effect was from the combination of naringin, neohesperidin, paeoniflorin and platycodin, better than that of the saline group (P < 0.01). However, there was no significant difference as compared with the aspirin group (P > 0.05). Paeoniflorin showed a dominant contribution to the formula, and platycodin the least. The combination of all active components exhibited synergism.
  
  
  CONCLUSIONS
  The results suggest that all the ingredients are efficacious constituents of the formula, and paeoniflorin shows a dominant contribution to the formula. More information about prescription compatibility can be obtained by the orthogonal simulation method.
authors: Center for Drug of Clinical Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors: Jun-Chao
authors: JC
authors: Chen
authors: Lu-Jin
authors: LJ
authors: Li
authors: Shi-Mei
authors: SM
authors: Wen
authors: Ying-Chun
authors: YC
authors: He
authors: Hong-Xia
authors: HX
authors: Liu
authors: Qing-Shan
authors: QS
authors: Zheng
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001565:Benzoates / Q000302:isolation & purification / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D051379:Mice; D039821:Monoterpenes; D011208:Powders; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D006569:Hesperidin; D039821:Monoterpenes; D011208:Powders; D012503:Saponins; C008936:platycodin; C015423:peoniflorin; D006027:Glycosides; C005274:naringin; C546526:neohesperidin