[Establishment of protein expression profile of laser capture microdissection-purified nasopharyngeal carcinoma tissue].
OBJECTIVE
To establish the protein expression map of nasopharyngeal carcinoma (NPC), and provide a basis for proteomic study of NPC.


METHODS
Laser capture microdissection (LCM) was used to isolate cancer cells from NPC tissues. Two-dimensional gel electrophoresis(2-DE) was used to separate the total proteins of LCM purified NPC cells. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was performed to identify the proteins, and bioinformatics was used to construct the 2-DE database of NPC proteome.


RESULTS
A 2-DE reference map of NPC was established. On the 2-DE map, a total of (1 312+/-30) protein spots were detected, and 427 protein spots representing 241 non-redundant proteins were identified. The 2-DE database of NPC proteome was constructed. These data could be accessed at our website (http://www.xyproteomics.org).


CONCLUSIONS
A protein expression profile of LCM purified NPC tissues has been established for the first time, which provides useful information and source for proteomic study of NPC.
Peng
Fang
F
Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China.
Tang
Can'e
C
Li
Maoyu
M
Li
Cui
C
Chen
Ailan
A
Li
Feng
F
Zhang
Pengfei
P
Li
Meixiang
M
Xiao
Zhiqiang
Z
Chen
Zhuchu
Z
D019295:Computational Biology; D020869:Gene Expression Profiling; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009363:Neoplasm Proteins; D020543:Proteome