[Pharmacologic vitreolysis in diabetic rats].
OBJECTIVE
To evaluate the efficacy and safety of plasmin or hyaluronidase in the inducing of posterior vitreous detachment in diabetic rats.


METHODS
Forty SD rats were induced diabetes by Streptozotocin (STZ). Four weeks later, these rats were randomized into 4 groups: rats in group A received 5 U hyaluronidase intravitreal injection in right eyes; rats in group B received 0.5 U plasmin intravitreal injection in right eyes; rats in group C received 0.5 U plasmin +5 U hyaluronidase intravitreal injection in right eyes; rats in group D received BSS (balance salt solution) 2 microl intravitreal injection in right eyes. Clinical examination were performed at 1, 3, 7 days after injection. After 1 week, scanning electron microscope (SEM) was performed to judge whether the PVD was induced. ERG and histology were examined to evaluate the toxicity after the intravitreal injection of these two drugs.


RESULTS
No PVD was found in SEM disclosed group A and group D. Forty percent eyes were induced complete PVD in groups B, and one hundred percent eyes were induced complete PVD in group C. ERG and histology showed no toxicity changes in any group.


CONCLUSIONS
Intravitreal injection of 0.5 U plasmin + 5 U hyaluronidase can induce complete PVD without obvious toxicity in diabetic rats. Solo usage of plasmin or hyaluronidase can not induce complete PVD.
Luo
Min
M
Department of Ophthalmology, the Ninth Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200011, China.
Wang
Zhi-liang
ZL
Shi
Wo-dong
WD
Fan
Xian-qun
XQ
Xu
Guo-tong
GT
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D003930:Diabetic Retinopathy / Q000601:surgery; D005341:Fibrinolysin / Q000009:adverse effects* / Q000627:therapeutic use; D006821:Hyaluronoglucosaminidase / Q000009:adverse effects* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000473:pathology; D014822:Vitreous Body; D020255:Vitreous Detachment / Q000139:chemically induced*
D006821:Hyaluronoglucosaminidase; D005341:Fibrinolysin