title: Oral systemic administration of Ankaferd blood stopper has no short-term toxicity in an in vivo rabbit experimental model.
abstract: BACKGROUND
  Ankaferd blood stopper (ABS) is a standardized herbal extract obtained from 5 different plants. In Turkey, it has been approved for local topical applications in external postsurgical and postdental surgery bleedings. Ankaferd blood stopper, besides its hemostatic activity, has in vitro anti-infectious and antineoplastic actions.
  
  
  OBJECTIVE
  The aim of this study was to assess short-term hematological and biochemical safety following the oral systemic administration of ABS to rabbits.
  
  
  METHODS
  Twelve rabbits (aged 6-12 months) were included to test the safety of oral ABS. Animals were divided into 4 groups, which had ABS administered orally at doses of 1, 3, 6, and 9 mL, irrespective of their weight. The general well-being and feeding patterns of the animals were observed for a period of 7 days. Blood samples (5.5 mL) were obtained just before oral administration, on days 1 and 4.
  
  
  RESULTS
  During the observation period of 7 days, none of the animals showed any abnormal behavior or deviation from the normal. Acute mucosal toxicity, hematotoxicity, hepatotoxicity, nephrotoxicity, and biochemical toxicity were not observed during the short-term follow-up of the animals.
  
  
  CONCLUSIONS
  No signs of toxicity were observed in rabbits during short-term study with oral ABS administration.
authors: Department of SurgeryFaculty of Veterinary Medicine, Ankara UniversityAnkara, Turkey.
authors: Hasan
authors: H
authors: Bilgili
authors: Ozge
authors: O
authors: Captug
authors: Ali
authors: A
authors: Kosar
authors: Mevlut
authors: M
authors: Kurt
authors: Murat
authors: M
authors: Kekilli
authors: Ali
authors: A
authors: Shorbagi
authors: Ozlem Kar
authors: OK
authors: Kurt
authors: Oktay
authors: O
authors: Ozdemir
authors: Hakan
authors: H
authors: Goker
authors: Ibrahim Celalettin
authors: IC
authors: Haznedaroglu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004195:Disease Models, Animal; D010936:Plant Extracts / Q000633:toxicity*; D011817:Rabbits
chemical_list: D010936:Plant Extracts; C539751:ankaferd blood stopper