title: Differential response of Leydig cells in expressing 11beta-HSD type I and cytochrome P450 aromatase in male rats subjected to corticosterone deficiency.
abstract: Emerging evidence suggests that the glucocorticoid and estradiol are important for Leydig cell steroidogenesis and are regulated via aromatase for estradiol production and 11beta-HSD for oxidatively inactivating glucocorticoid. Although it is known that corticosterone deficiency impaired Leydig cell steroidogenesis, its effect on the expression of Leydig cell 11beta-HSD type I and aromatase are yet to be recognized. Following metyrapone-induced corticosterone deficiency, serum corticosterone and testosterone levels decrease, whereas serum estradiol remains unaltered. 11beta-HSD type I mRNA and its activity was decreased by corticosterone deficiency, whereas the activity and mRNA of aromatase remains unaltered. Simultaneous administration of corticosterone prevented its deficiency-induced changes of 11beta-HSD type I in Leydig cells. Our results show that metyrapone-induced corticosterone deficiency impairs Leydig cell 11beta-HSD enzyme activity and 11beta-HSD type I mRNA expression, and the Leydig cells need to maintain their intracellular concentration of corticosterone for a normal function.
authors: Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, Tamil Nadu, India.
authors: Chandrakesan
authors: C
authors: Parthasarathy
authors: Sambandam
authors: S
authors: Yuvaraj
authors: Ramachandran
authors: R
authors: Ilangovan
authors: Panneerselvam
authors: P
authors: Janani
authors: Palaniyandi
authors: P
authors: Kanagaraj
authors: Muthusamy
authors: M
authors: Balaganesh
authors: Bhaskaran
authors: B
authors: Natarajan
authors: Sivanandane
authors: S
authors: Sittadjody
authors: Karundevi
authors: K
authors: Balasubramanian
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000378:metabolism*; D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood / Q000172:deficiency* / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood
chemical_list: D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D001141:Aromatase; D003345:Corticosterone; D008797:Metyrapone