Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages.
OBJECTIVE
The root of Scutellaria baicalensis Georgi (Labiatae), also known as Scutellariae Radix, possesses anticancer, antiviral, and anti-inflammatory properties. And it is one of the most widespread herbal remedies used in Oriental medicine. In the present study, we investigated the effects of Scutellariae Radix water extract (SR) on proinflammatory mediators secreted from lipopolysaccharide (LPS)-induced RAW 264.7 macrophages.


METHODS
Cell viability was assessed by MTT assay and nitric oxide (NO) concentration in the cultured medium was determined by the Griess reaction. Various Cytokines released from LPS-induced Raw 264.7 cells were measured in the cell culture supernatants using a multiplex bead array assay based on xMAP technology.


RESULTS
We found that SR significantly inhibited the production of NO, interleukin (IL)-3, IL-6, IL-10, IL-12p40, IL-17, interferon-inducible protein (IP)-10, keratinocyte-derived chemokine (KC), and vascular endothelial growth factor (VEGF) in LPS-induced RAW 264.7 cells at the concentrations of 25, 50, 100, 200 microg/ml (p<0.05).


CONCLUSIONS
These results suggest that SR has anti-inflammatory activity related with its inhibition of NO, cytokine, chemokine, and growth factor production in macrophages.
Yoon
Seok-Bin
SB
Dept. of Herbology, College of Oriental Medicine, Kyungwon University, Seongnam 461-701, Republic of Korea.
Lee
Young-Jong
YJ
Park
Seong Kyu
SK
Kim
Ho-Cheol
HC
Bae
Hyunsu
H
Kim
Hyung Min
HM
Ko
Seong-Gyu
SG
Choi
Ho Young
HY
Oh
Myung Sook
MS
Park
Wansu
W
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D031364:Scutellaria baicalensis; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide