title: Gross pathology, blood chemistry, lipid and peroxide contents in rainbow trout ( Oncorhynchus mykiss Walbaum) affected by experimental Arcobacter cryaerophilus infection at low water temperature.
abstract: Arcobacter cryaerophilus was isolated from naturally infected rainbow trout ( Oncorhynchus mykiss Walbaum), and its pathogenicity was tested by intramuscular injection using healthy 1-year-old rainbow trout under cold-water conditions (at 5 degrees C). The lethal dosage of 50% end point (LD 50 ) for A. cryaerophilus was calculated as 7.79 x 10 5 viable cells. Experimental infection caused gross clinical abnormalities such as fallen scales, exophthalmia, oedema in injection region and at the base of fins, pale gills, kidney necrosis, hyperaemic areas in pale liver, haemorrhagic spots in heart, elongated spleen and swollen gallbladder. Activities of aspartate aminotransferase and alkaline phosphatase, and concentrations of glucose, total protein, albumin, cholesterol, triglyceride and calcium in the serum of the experimentally infected rainbow trout were significantly decreased compared with the healthy fish. Positive correlations were observed among blood parameters. Total lipid weights increased in the brain, muscle and liver tissues of infected fish and dropped in the gill and spleen tissues. Lipid peroxide contents in the brain, liver, kidney, spleen, muscle and gill tissues of infected rainbow trout were significantly higher than in healthy animals. The present work shows that A. cryaerophilus can be moderately virulent for rainbow trout at low water temperature, and changes in lipid and lipid peroxide contents of tissues and blood indices can highlight barely detectable effects of A. cryaerophilus infection in rainbow trout under laboratory conditions. However, the application of these indices in farm biomonitoring using rainbow trout will need more detailed studies and a careful consideration of the environmental parameters.
authors: Kastamonu University, Education Faculty, 37200 Kastamonu, Turkey. saydin@kastamonu.edu.tr
authors: Seyit
authors: S
authors: Aydin
authors: Hayati
authors: H
authors: Gure
authors: Hasan
authors: H
authors: Cakici
authors: Serhat
authors: S
authors: Colakoglu
authors: Recep
authors: R
authors: Bircan
mesh_terms: D000818:Animals; D020640:Arcobacter / Q000502:physiology*; D003080:Cold Temperature; D005393:Fish Diseases / Q000097:blood* / Q000382:microbiology / Q000473:pathology*; D016905:Gram-Negative Bacterial Infections / Q000097:blood / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D017686:Oncorhynchus mykiss; D014867:Water
chemical_list: D014867:Water