[Overexpression of Tiam1 gene and its relationship with invasive and metastatic ability of nasopharyngeal carcinoma].
OBJECTIVE
To explore biological aspects of Tiam1 gene expression in nasopharyngeal carcinoma cells.


METHODS
Tiam1/C1199HA expression plasmids were transfected into nasopharyngeal carcinoma cells of C666-1 and CNE1 by lipofectamine2000. RT-PCR, real-time PCR and Western blot Analyses were performed to evaluate the expression of Tiam1 mRNA and protein levels, respectively. In vitro cell adhesion, wound healing and matrigel invasion assays were used to study the biological impact of Tiam1 on cell adhesion, mobility and invasion.


RESULTS
Tiam1 over expression significantly increased the abilities of adhesion, migratory and invasion of C666-1 and CNE1 cells, comparing with that of the control untransfected cells (P < 0.05).


CONCLUSIONS
Tiam1 expression correlates with the invasion and metastasis of nasopharyngeal carcinoma cells.
Zhang
Xiang-mei
XM
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Ding
Yi
Y
Chen
Juan-zhi
JZ
Jin
He
H
Yu
Li-na
LN
Li
Yu-fa
YF
Ding
Yan-qing
YQ
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1
D020662:Guanine Nucleotide Exchange Factors; D012333:RNA, Messenger; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C087334:TIAM1 protein, human