Development and characterization of a monoclonal antibody against Taura syndrome virus.
We produced a panel of monoclonal antibodies (MAbs) from the fusion of Taura syndrome virus variants from Belize (TSV-BZ) immunized BALB/cJ mouse spleen cells and non-immunoglobulin secreting SP2/0 mouse myeloma cells. One antibody, 2C4, showed strong specificity and sensitivity for TSV in dot-blot immunoassay and immunohistochemistry (IHC) analysis. The MAb reacted against native TSV-BZ, TSV variants from Sinaloa, Mexico (TSV-SI) and TSV variants from Hawaii (TSV-HI) in dot-blot immunoassay. By IHC, the antibody identified the virus in a pattern similar to the digoxigenin-labelled TSV-cDNA probe for the TSV-BZ, TSV-HI and TSV-SI variants, but not for the TSV variants from Venezuela (TSV-VE) and the TSV variants from Thailand (TSV-TH). MAb 2C4 did not react against other shrimp pathogens or with normal shrimp tissue. Western blot analysis showed a strong reaction against CP2, a region of high antigenic variability amongst TSV variants. This antibody has potential diagnostic application in detection and differentiation of certain TSV biotypes.
Côté
I
I
Department of Veterinary Sciences and Microbiology, The University of Arizona, Tucson, AZ 85721, USA. aquavet@gmail.com
Poulos
B T
BT
Redman
R M
RM
Lightner
D V
DV
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D056629:Dicistroviridae / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D033561:Penaeidae / Q000821:virology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral