title: [The construction of a preliminary genetic linkage map in the Japanese scallop Mizuhopecten yessoensis].
abstract: AFLP markers were used to construct the primary linkage map in a family of Mizuhopecten yessoensis. A total of 1 855 markers were generated in two parents and 52 progenies of the mapping family by using 56 AFLP primer combinations. Among the 1 855 markers, 598 were polymorphic and 354 were in agreement with the Mendelian segregating ratio of 1:1. Markers segregated according to Mendelian 1:1 ratio (P>0.05) and 23 distorted markers (0.01<P<0.05) were used in linkage analysis. The male map was composed of 178 markers in 22 linkage groups, covering a total of 1 263.0 cM with the average interval of 8.1 cM between markers, and the observed coverage was 79.4%. The female map was composed of 163 markers in 22 linkage groups, covering a total of 1 259.5 cM with the average interval of 8.9 cM between markers, and the observed coverage was 76.8%. AFLP markers showed an uneven distribution on the linkage group and there were a lot of markers with a distance of 0.
authors: Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. cnliu51@tom.com
authors: Wei-Dong
authors: WD
authors: Liu
authors: Xiang-Bo
authors: XB
authors: Bao
authors: Wen-Tao
authors: WT
authors: Song
authors: Zun-Chun
authors: ZC
authors: Zhou
authors: Chong-Bo
authors: CB
authors: He
authors: Xing-Ju
authors: XJ
authors: Yu
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D003433:Crosses, Genetic; D004247:DNA / Q000032:analysis; D005260:Female; D008040:Genetic Linkage; D005828:Genetics, Population; D007564:Japan; D008297:Male; D049897:Pectinidae / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA