title: Qingkailing injection attenuates apoptosis and neurologic deficits in a rat model of intracerebral hemorrhage.
abstract: OBJECTIVE
  Traditional Chinese herb Angong Niuhuang Pill (AGNHP) is a famous preparation for neurological diseases; Qingkailing injection (QKL), an extract of AGNHP has similar clinical applications. This investigation was designed to further elucidate the neuroprotective effect of QKL on intracerebral hemorrhage (ICH).
  
  
  METHODS
  ICH was produced in adult Sprague-Dawley rats by injection of collagenase IV. Three incremental doses of QKL injection including low-(0.5 ml/kg), moderate-(1 ml/kg) and high-dosage (2 ml/kg) were administered twice, 3 and 12h following ICH. TUNEL and caspase-3 activity were measured at 1d after ICH, and apomorphine-induced rotation was evaluated at 1d, 7d, 14 d and 28 d.
  
  
  RESULTS
  Administration of high-dose QKL inhibited TUNEL positive cells (p<0.05) and caspase-3 activity (p<0.05) at 1d following ICH, and reduced apomorphine-induced rotation at 1d (p<0.01), 7d, 14 d and 28 d (p<0.05), compared with the controls. However, QKL in a low or moderate dose had no such effect.
  
  
  CONCLUSIONS
  QKL reduced brain damage of intracerebral hemorrhage through inhibiting apoptosis, which suggested a potential intervention for ICH patients.
authors: Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, PR China.
authors: Lei
authors: L
authors: Lv
authors: Yi
authors: Y
authors: Liu
authors: Hui-fen
authors: HF
authors: Shi
authors: Qiang
authors: Q
authors: Dong
mesh_terms: D000818:Animals; D001058:Apomorphine; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects*; D061945:Caspase Inhibitors; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000188:drug therapy*; D017364:Collagenases; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D007275:Injections, Intravenous; D019684:Magnoliopsida; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D061945:Caspase Inhibitors; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C504928:Qingkailing; D017364:Collagenases; D001058:Apomorphine