title: Serum bile acid profiling reflects enterohepatic detoxification state and intestinal barrier function in inflammatory bowel disease.
abstract: OBJECTIVE
  To determine free and conjugated serum bile acid (BA) levels in inflammatory bowel disease (IBD) subgroups with defined clinical manifestations.
  
  
  METHODS
  Comprehensive serum BA profiling was performed in 358 IBD patients and 310 healthy controls by liquid chromatography coupled to electrospray ionization tandem mass spectrometry.
  
  
  RESULTS
  Serum levels of hyodeoxycholic acid, the CYP3A4-mediated detoxification product of the secondary BA lithocholic acid (LCA), was increased significantly in Crohn's disease (CD) and ulcerative colitis (UC), while most other serum BA species were decreased significantly. Total BA, total BA conjugate, and total BA glycoconjugate levels were decreased only in CD, whereas total unconjugated BA levels were decreased only in UC. In UC patients with hepatobiliary manifestations, the conjugated primary BAs glycocholic acid, taurocholic acid, and glycochenodeoxycholic acid were as significantly increased as the secondary BAs LCA, ursodeoxycholic acid, and tauroursodeoxycholic acid compared to UC patients without hepatobiliary manifestations. Finally, we found that in ileocecal resected CD patients, the unconjugated primary BAs, cholic acid and chenodeoxycholic acid, were increased significantly compared to controls and patients without surgical interventions.
  
  
  CONCLUSIONS
  Serum BA profiling in IBD patients that indicates impaired intestinal barrier function and increased detoxification is suitable for advanced diagnostic characterization and differentiation of IBD subgroups with defined clinical manifestations.
authors: Institute of Clinical Chemistry and Laboratory Medicine, Regensburg University Medical Center, Regensburg, Germany.
authors: Carsten
authors: C
authors: Gnewuch
authors: Gerhard
authors: G
authors: Liebisch
authors: Thomas
authors: T
authors: Langmann
authors: Benjamin
authors: B
authors: Dieplinger
authors: Thomas
authors: T
authors: Mueller
authors: Meinhard
authors: M
authors: Haltmayer
authors: Hans
authors: H
authors: Dieplinger
authors: Alexandra
authors: A
authors: Zahn
authors: Wolfgang
authors: W
authors: Stremmel
authors: Gerhard
authors: G
authors: Rogler
authors: Gerd
authors: G
authors: Schmitz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001647:Bile Acids and Salts / Q000032:analysis / Q000097:blood; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood* / Q000145:classification / Q000175:diagnosis / Q000503:physiopathology*; D007422:Intestines / Q000473:pathology* / Q000502:physiology / Q000503:physiopathology* / Q000601:surgery; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D021241:Spectrometry, Mass, Electrospray Ionization; D055815:Young Adult
chemical_list: D001647:Bile Acids and Salts