Ionic-complementary peptide matrix for enzyme immobilization and biomolecular sensing.
A novel electrochemical biosensing platform is described using biocompatible, self-assembled ionic-complementary peptide nanofibers. The compatibility of a graphite electrode modified by these peptide nanofibers with enzymes is demonstrated using a model enzyme glucose oxidase (GOx). A glucose biosensor has been successfully fabricated by incorporating this enzyme into the modified electrode. From measurement of its electrode response and sensitivity, this nanofiber-modified electrode shows promise as an enzyme-based biosensor. The findings presented here demonstrate excellent potential of the use of ionic-complementary peptides to modify electrode surfaces for biomolecular sensing and diagnostics.
Yang
Hong
H
Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
Fung
Shan-Yu
SY
Pritzker
Mark
M
Chen
P
P
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008962:Models, Theoretical; D010455:Peptides / Q000737:chemistry*
D004800:Enzymes, Immobilized; D010455:Peptides