In vitro biological activity of Salvia leriifolia benth essential oil relevant to the treatment of Alzheimer's disease.
In this study the chemical composition, cholinesterase inhibitory property and anti-inflammatory activity of S. leriifolia Benth. essential oil was evaluated for the first time. GC and GC-MS analysis revealed the presence of camphor (10.5%), 1,8-cineole (8.6%), camphene (6.2%) and alpha-pinene (4.7%) as main constituents. S. leriifolia oil exhibited a promising antioxidant activity by DPPH assay with an IC(50) 2.26 microL/mL. Interesting cholinesterase inhibitory activity was also found with IC(50) values of 0.32 and 0.29 microL/mL for acetylcholinesterase (AChE) and butyrrylcholinesterase (BChE), respectively. Moreover, this oil inhibited LPS-induced NO production with an IC(50) value of 165 microg/mL. The absence of cytotoxicity at 1000 microg/mL was evaluated by MTT assay in 142BR cells.
Loizzo
Monica Rosa
MR
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Nutrition and Health Sciences, University of Calabria, Italy. mr.loizzo@unical.it
Menichini
Federica
F
Tundis
Rosa
R
Bonesi
Marco
M
Conforti
Filomena
F
Nadjafi
Farsad
F
Statti
Giancarlo Antonio
GA
Frega
Natale Giuseppe
NG
Menichini
Francesco
F
D000110:Acetylcholinesterase / Q000378:metabolism; D000544:Alzheimer Disease / Q000188:drug therapy*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002091:Butyrylcholinesterase / Q000378:metabolism; D002460:Cell Line; D002849:Chromatography, Gas; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D027544:Salvia / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D008070:Lipopolysaccharides; D009822:Oils, Volatile; D009569:Nitric Oxide; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase