title: Production of an egg yolk antibody against Parietaria judaica 2 allergen.
abstract: Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.
authors: Università di Palermo, Sezione di Biologia e Genetica, Dipartimento di Biopatologia e Metodologie Biomediche, Palermo, Italy. ricale@unipa.it
authors: R
authors: R
authors: Alessandro
authors: A
authors: A
authors: Gallo
authors: M
authors: M
authors: Barranca
authors: S
authors: S
authors: Principe
authors: S
authors: S
authors: Taverna
authors: G
authors: G
authors: Duro
authors: G
authors: G
authors: Cassata
authors: M
authors: M
authors: Becchi
authors: S
authors: S
authors: Fontana
authors: G
authors: G
authors: De Leo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D002645:Chickens / Q000276:immunology* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk / Q000378:metabolism*; D005260:Female; D007136:Immunoglobulins / Q000276:immunology* / Q000378:metabolism; D032403:Parietaria / Q000737:chemistry* / Q000276:immunology; D011058:Pollen / Q000276:immunology
chemical_list: D000906:Antibodies; D052179:Antigens, Plant; C016512:IgY; D007136:Immunoglobulins