[Expression of the voltage-dependent anion channel gene in human ejaculated spermatozoa].
OBJECTIVE
To identify the genes involved in sperm motility.


METHODS
We hybridized asthenospermia and normal motile sperm cDNA samples with the human whole genome Affymetrix chip to screen differentially expressed genes. Then we detected the mRNA expressions of the voltage-dependent anion channel genes (VDACs) in human organs and spermatozoa by RT-PCR and compared their expressions in the poor and normal motility spermatozoa.


RESULTS
Differentially expressed genes VDACs were identified by analysis of the hybridization signals, including the 3 subtypes VDAC1, VDAC2 and VDAC3. The expression of VDAC2 mRNA was significantly decreased in the poor motility sperm (0.568 +/- 0.036), as compared with the healthy men (0.803 +/- 0.043, P < 0.01).


CONCLUSIONS
The decreased expression of VDAC2 in the ejaculated spermatozoa is possibly associated with the reduction of sperm motility.
Xu
Xiang
X
Key Laboratory of Male Reproductive and Genetic Medicine, Peking University Shenzhen Hospital, Shenzhen, Guang-dong 518036, China. xuxiang_hard@163.com
Wang
Yong
Y
Yu
Zhou
Z
Chen
Jing
J
Guo
Meng
M
Gui
Yao-ting
YT
Cai
Zhi-ming
ZM
D055028:Comparative Genomic Hybridization; D015870:Gene Expression; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D050996:Voltage-Dependent Anion Channel 2 / Q000235:genetics*
D050996:Voltage-Dependent Anion Channel 2