Gaussia luciferase variant for high-throughput functional screening applications.
Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.
Maguire
Casey A
CA
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston Massachusetts 02115, USA.
Deliolanis
Nikolaos C
NC
Pike
Lisa
L
Niers
Johanna M
JM
Tjon-Kon-Fat
Lee-Ann
LA
Sena-Esteves
Miguel
M
Tannous
Bakhos A
BA
D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D033342:Copepoda / Q000201:enzymology*; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D008156:Luciferases