title: Biosynthesis of salinosporamides from alpha,beta-unsaturated fatty acids: implications for extending polyketide synthase diversity.
abstract: A new series of coenzyme A-tethered polyketide synthase extender units were discovered in relation to the biosynthesis of the salinosporamide family of anticancer agents from the marine bacterium Salinispora tropica. In vivo and in vitro experiments revealed that the crotonyl-CoA reductase/carboxylase SalG has broad substrate tolerance toward 2-alkenyl-CoAs that give rise to the salinosporamide C-2 substitution pattern.
authors: Scripps Institution of Oceanography, University of California at San Diego, La Jolla, California 92093-0204, USA.
authors: Yuan
authors: Y
authors: Liu
authors: Christopher
authors: C
authors: Hazzard
authors: Alessandra S
authors: AS
authors: Eustáquio
authors: Kevin A
authors: KA
authors: Reynolds
authors: Bradley S
authors: BS
authors: Moore
mesh_terms: D000192:Actinomycetales / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D007769:Lactams / Q000737:chemistry / Q000378:metabolism*; D048630:Polyketide Synthases / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D005231:Fatty Acids, Unsaturated; D007769:Lactams; C015357:methylmalonyl-coenzyme A; D048630:Polyketide Synthases