Clone-based functional genomics.
Annotated genomes have provided a wealth of information about gene structure and gene catalogs in a wide range of species. Taking advantage of these developments, novel techniques have been implemented to investigate systematically diverse aspects of gene and protein functions underpinning biology processes. Here, we review functional genomics applications that require the mass production of cloned sequence repertoires, including ORFeomes and silencing tag collections. We discuss the techniques employed in large-scale cloning projects and we provide an up-to-date overview of the clone resources available for model plant species and of the current applications that may be scaled up for systematic plant gene studies.
Bleys
Annick
A
Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), Gent, Belgium.
Karimi
Mansour
M
Hilson
Pierre
P
D000818:Animals; D019976:Cloning, Organism / Q000379:methods*; D023281:Genomics / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D030781:Organisms, Genetically Modified / Q000235:genetics* / Q000502:physiology*; D010944:Plants / Q000235:genetics / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods; D025941:Protein Interaction Mapping / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D014162:Transfection / Q000379:methods; D015003:Yeasts / Q000235:genetics / Q000378:metabolism