[Molecular cloning and protein structure analyses of red/blue light chimeric photoreceptor from Allantodia dilatata (Bl.) Ching].
Phytochrome 3 (PHY3) is a novel chimeric photoreceptor that can respond to both red/far red and blue light. Using this photoreceptor, some cryptogams could enhance light sensitivity under low light environment. But PHY3 sequence information is still extremely limited. In the present study, a full-length PHY3 genomic sequence was cloned from a fern Allantodia dilatata (Bl.) Ching by inverse PCR approaches. Sequence analysis showed that introns were absent in the gene. It contained a 4 278 bp open reading frame, encoding a deduced protein of 1 425 amino acid residues with a theoretical isoelectric point (pI) of 6.29 and a calculated molecular mass about 157 kDa. Protein domain search and structure analyses indicated that PHY3 originated from the recombination of two different photoreceptors. Its N-terminal section consisted of a putative functional phytochrome chromophore-binding domain including PAS, GAF, and PHY, whereas the C-terminal region possessed a nearly complete phototropin motif with two LOV and one STKc domains.
Yang
Yong-Xia
YX
Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. yyx624@126.com
Gao
Lei
L
Wang
Ting
T
D003001:Cloning, Molecular; D003116:Color; D019143:Evolution, Molecular; D029624:Ferns / Q000235:genetics*; D008027:Light; D008958:Models, Molecular; D055519:Photoreceptors, Plant / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D010834:Phytochrome / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
D055519:Photoreceptors, Plant; D011993:Recombinant Fusion Proteins; D010834:Phytochrome