title: Regulation of intracerebral arteriolar tone by K(v) channels: effects of glucose and PKC.
abstract: Voltage-gated potassium (K(v)) channels in vascular smooth muscle cells (VSMC) are critical regulators of membrane potential and vascular tone. These channels exert a hyperpolarizing influence to counteract the depolarizing effects of intraluminal pressure and vasoconstrictors. However, the contribution of K(v) channel activity to the functional regulation of cerebral (parenchymal) arterioles within the brain is not known. Thus K(v) channel properties in parenchymal arteriolar SMCs were characterized. Isolated, pressurized parenchymal arterioles and arterioles in cortical brain slices exhibited robust constriction in the presence of the K(v) channel inhibitor 4-aminopyridine (4-AP). 4-AP also decreased the amplitude of K(v) currents recorded from SMCs. The steady-state activation and inactivation properties of K(v) currents suggested that these channels are composed of K(v)1.2 and 1.5 subunits, which was confirmed by RT-PCR. K(v) channels can be regulated by extracellular glucose, which may be involved in the functional hyperemic response in the brain. Thus the effects of glucose on K(v) channel activity and arteriolar function were investigated. Elevation of glucose from 4 to 14 mM significantly decreased the peak K(v) current amplitude and constricted arterioles. Arteriolar constriction was prevented by inhibition of protein kinase C (PKC), consistent with previous studies showing enhanced PKC activity in the presence of elevated glucose. In cortical brain slices, the dilation generated by neuronal activity induced by electrical field stimulation was decreased by 54% in 14 mM glucose when compared with the dilation in 4 mM glucose. In anesthetized mice the whisker stimulation-induced increase in local cerebral blood flow was also significantly decreased in 14 mM glucose, and this effect was similarly prevented by PKC inhibition. These findings point to a critical role for K(v) channels in the regulation of intracerebral arteriolar function and suggest that changes in perivascular glucose levels could directly alter vascular diameter resulting in a modulation of local cerebral blood flow.
authors: Dept. of Pharmacology, University of Vermont, Burlington, VT 05405, USA.
authors: Stephen V
authors: SV
authors: Straub
authors: Helene
authors: H
authors: Girouard
authors: Paul E
authors: PE
authors: Doetsch
authors: Rachael M
authors: RM
authors: Hannah
authors: M Keith
authors: MK
authors: Wilkerson
authors: Mark T
authors: MT
authors: Nelson
mesh_terms: D000818:Animals; D054022:Cerebrum / Q000098:blood supply*; D004594:Electrophysiology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism / Q000494:pharmacology*; D021122:Protein Subunits; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D011493:Protein Kinase C; D005947:Glucose