Isolation and characterization of a novel EAR-motif-containing gene GmERF4 from soybean (Glycine max L.).
Transcriptional repressors are emerging as central regulators of development and stress responses in different organisms. The ERF-associated amphiphilic repression (EAR) motif was identified as essential for transcriptional repression. To gain a better understanding of this type of protein, we reported here a novel GmERF4 protein from soybean. Sequence alignment showed that GmERF4 contains one AP2/ERF domain, two putative nuclear localization signal regions and one EAR motif. The GmERF4 protein was preferentially localized to the nucleus of onion epidermis cells and bound specifically to the GCC box and DRE/CRT element in vitro. Furthermore, the expression of GmERF4 was induced by ethylene, JA, SA, cold, salt, drought, and soybean mosaic virus, and repressed by ABA. Constitutive expression of GmERF4 in transgenic tobacco plants increased tolerance to salt and drought stresses compared with wild-type plants, but did not exhibit detectable resistance against bacterial infection.
Zhang
Gaiyun
G
Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China.
Chen
Ming
M
Chen
Xueping
X
Xu
Zhaoshi
Z
Li
Liancheng
L
Guo
Jiaming
J
Ma
Youzhi
Y
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D020218:Response Elements; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000276:immunology
D004268:DNA-Binding Proteins; D010940:Plant Proteins