title: Quantitative lung T cell responses aid the rapid diagnosis of pulmonary tuberculosis.
abstract: BACKGROUND
  The diagnosis of smear-negative pulmonary tuberculosis (TB) is problematic. There are limited data on the profile of alveolar TB antigen-specific T cells, and their utility for the rapid immunodiagnosis of pulmonary TB is unclear.
  
  
  METHODS
  Antigen-specific interferon gamma (IFNgamma) responses to the RD-1 antigens ESAT-6 and CFP-10 (T-SPOT.TB and QuantiFERON-TB-Gold-In-Tube), heparin-binding haemagglutinin and purified protein derivative were evaluated, using alveolar lavage cells, in 91 consecutively recruited South African patients suspected of having TB.
  
  
  RESULTS
  Of 85 evaluable patients (29% HIV+), 24, 11, 48 and 2 had definite TB, probable TB, non-TB and an uncertain diagnosis, respectively. Between 34% (T-SPOT.TB) and 41% (QuantiFERON-TB-Gold-In-Tube) of all test results were inconclusive. Failure of the positive control was significantly higher with the QuantiFERON-TB-Gold-In-Tube than with T-SPOT.TB (85% vs 46% of inconclusive results; p = 0.001). Using staphylococcal enterotoxin B, compared with phytohaemagglutinin, substantially reduced failure of the positive control (25% to 3%; p = 0.02). In evaluable samples, when the definite and non-TB groups were used for outcome analysis, the percentage sensitivity, specificity, positive predictive value and negative predictive value for T-SPOT.TB (> or = 20 spots/million alveolar mononuclear cells) and QuantiFERON-TB-Gold-In-Tube (0.35 IU/ml) were 89, 94, 89 and 94% (n = 55) and 55, 86, 77 and 69% (n = 46), respectively. Rapid diagnosis of TB was achieved more frequently with T-SPOT.TB than with smear microscopy (14/24 (58%) vs. 7/24 (29%) of definite TB cases; p = 0.02). Heparin-binding haemagluttinin and purified protein derivative alveolar lymphocyte IFNgamma responses had poor performance outcomes.
  
  
  CONCLUSIONS
  Provided evaluable results are obtained, the RD-1, but not the heparin-binding haemagglutinin or purified protein derivative, alveolar lymphocyte IFNgamma ELISPOT response is a useful rapid immunodiagnostic test for TB. However, test utility in high-burden settings may be limited by the high proportion of inconclusive results.
authors: Lung Infection and Immunity Unit & CTBRI, UCT Lung Institute & Division of Pulmonology, Department of Medicine, University of Cape Town, South Africa. keertan.dheda@uct.ac.za
authors: K
authors: K
authors: Dheda
authors: R N
authors: RN
authors: van Zyl-Smit
authors: R
authors: R
authors: Meldau
authors: S
authors: S
authors: Meldau
authors: G
authors: G
authors: Symons
authors: H
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authors: Khalfey
authors: N
authors: N
authors: Govender
authors: V
authors: V
authors: Rosu
authors: L A
authors: LA
authors: Sechi
authors: A
authors: A
authors: Maredza
authors: P
authors: P
authors: Semple
authors: A
authors: A
authors: Whitelaw
authors: H
authors: H
authors: Wainwright
authors: M
authors: M
authors: Badri
authors: R
authors: R
authors: Dawson
authors: E D
authors: ED
authors: Bateman
authors: A
authors: A
authors: Zumla
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D007371:Interferon-gamma