Genes on rat chromosomes 3, 5, 10, and 16 are linked with facets of metabolic syndrome.
WOKW (Wistar Ottawa Karlsburg W) rats develop metabolic syndrome closely resembling human disorder. In crossing studies between disease-prone WOKW and disease-resistant DA (Dark Agouti) rats, several quantitative trait loci (QTLs) were mapped. To prove the in vivo relevance of QTLs, congenic DA.WOKW rats, briefly termed DA.3aW, DA.3bW, DA.5W, DA.10W, and DA.16W, were generated by transferring chromosomal regions of WOKW chromosomes 3, 5, 10, and 16 onto DA genetic background. Male (n=12) and female (n=12) rats of each congenic strain and their parental strain DA were characterized for adiposity index (AI), serum leptin, and serum insulin as well as serum cholesterol and serum triglycerides as single facets of metabolic syndrome at the age of 30 weeks. The data showed a significant higher AI for male and female DA.3aW and female DA.16W compared with DA. Serum leptin was significantly elevated in male and female DA.3aW, DA.10W, and DA.16W rats in comparison with DA. Rats of both sexes of DA.10W and female DA.16W showed significantly elevated serum insulin in comparison to DA. Female rats of all congenics had significantly higher serum cholesterol compared with DA, while males did not differ. Finally, triglycerides were only elevated in male DA.16W. The results demonstrate an involvement of WOKW chromosomes 3, 5, 10, and 16 in developing facets of the metabolic syndrome.
Baguhl
Romy
R
Department of Laboratory Animal Science, Medical Faculty, University Greifswald, Karlsburg, Germany.
Wilke
Barbara
B
Klöting
Nora
N
Klöting
Ingrid
I
D050154:Adiposity / Q000235:genetics; D000818:Animals; D020296:Animals, Congenic; D002784:Cholesterol / Q000097:blood; D033481:Chromosomes, Mammalian; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D005260:Female; D020022:Genetic Predisposition to Disease; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D010641:Phenotype; D040641:Quantitative Trait Loci; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D013045:Species Specificity; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
D007328:Insulin; D020738:Leptin; D014280:Triglycerides; D002784:Cholesterol