The design, synthesis and evaluation of hypoxia-activated pro-oligonucleotides.
Hypoxia-activated pro-oligonucleotides were synthesized through the commercial phosphoramidite method, and could be readily cleaved to form normal oligos with good hypoxia selectivity in vitro under the effect of reductases, as well as in tumor cell extract.
Zhang
Nan
N
Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, PR China.
Tan
Chunyan
C
Cai
Puqin
P
Zhang
Peizhuo
P
Zhao
Yufen
Y
Jiang
Yuyang
Y
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015195:Drug Design; D004926:Escherichia coli / Q000201:enzymology; D019793:Fluorescein / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D000860:Hypoxia; D009601:Nitroreductases / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011355:Prodrugs / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling
D009841:Oligonucleotides; D011355:Prodrugs; D009601:Nitroreductases; D019793:Fluorescein