[The preparation of nano-hydroxyapatite and preliminary observation on its effects on the occlusion of dentinal tubule].
OBJECTIVE
To synthesize nano-hydroxyapatite (nano-HA) and evaluate its effect on the occlusion of dentinal tubules in vitro, and therefore provide evidence for the clinical application.


METHODS
(NH(4))(2)HPO(4) and Ca(NO3)2 were used to form nano-HA, which was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrometer (FTIR) and X-ray diffractometer (XRD) respectively. Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups, which were control group, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group, and nano-HA group. After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days, the surface of slab dentin was observed using SEM.


RESULTS
SEM, TEM, FTIR, and XRD tests showed that nano-HA was synthesized successfully. SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group.


CONCLUSIONS
In comparison with CPP-ACP, nano-HA could occlude dentinal tubules more effectively in vitro.
Wang
Zhe-Jun
ZJ
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Sa
Yue
Y
Ma
Xiao
X
Wang
Yi-Ning
YN
Jiang
Tao
T
D002364:Caseins; D003764:Dental Materials / Q000138:chemical synthesis*; D003804:Dentin; D003807:Dentin Sensitivity / Q000628:therapy; D017886:Durapatite / Q000138:chemical synthesis*; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures; D013674:Technology, Dental
D002364:Caseins; D003764:Dental Materials; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D017886:Durapatite