Genetic transformation of Nepalese spring wheat (Triticum aestivum L.) cultivars with ipt gene under the regulation of a senescence enhanced promoter from maize.
Two Nepalese spring wheat cultivars were transformed with an ipt gene from Agrobacterium tumefaciens under the control of the senescence inducible promoter pSEE1 from maize using biolistic method. The resulting transgenic lines, one from Pasang Lahmu and seven from Annapurna-1, were studied for the expression of the transgene and the phenotype characters like chlorophyll content, chlorophyll a/b ratio, PS II quantum yield and other parameters of agronomic importance. Analysis of transgene expression by RT-PCR revealed very weak or no signal at all, indicating either partial or complete silencing of the transgene in the lines tested. None of the plants exhibited a phenotype that was significantly different from the respective azygous controls.
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Department of Environmental Sciences, Institute of Agriculture and Animal Sciences, Rampur Campus, Rampur, Chitawan, Nepal.
Bhattarai
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Beck
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Fettig
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D000375:Aging / Q000235:genetics*; D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D018556:Crops, Agricultural / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009390:Nepal; D018515:Plant Leaves / Q000502:physiology; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic; D019076:Transgenes; D014908:Triticum / Q000033:anatomy & histology / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase