title: A novel model of bone-metastatic prostate cancer in immunocompetent mice.
abstract: BACKGROUND
  Bone metastasis is a frequent and catastrophic consequence of prostate cancer for which only palliative treatment is available. Animal models of bone metastatic prostate cancer are necessary for understanding disease mechanisms but few models exist.
  
  
  METHODS
  We have used the murine prostate carcinoma cell line RM1 to generate a bone metastatic model of prostate cancer. Repeated intracardiac injection of RM1 cells followed by isolation of cells from bone tumors has yielded a cell line with strong bone-metastatic potential, RM1 bone metastatic (BM).
  
  
  RESULTS
  This cell line metastasizes to multiple bony sites in over 95% of injected C57BL/6 mice and is far less tropic to soft tissues. Bone tumors produced by the RM1(BM) cell line show no preference for particular skeletal sites as most bones are affected. Histology, and micro-computed tomography show that RM1(BM) cells form osteolytic tumors, but with evidence of osteoblastic changes. In vitro the RM1 cells express E-cadherin but not vimentin, do not form colonies in soft agar, are non-invasive but are more motile than the parent cell line.
  
  
  CONCLUSIONS
  This model provides a novel means for identifying cellular and molecular mechanisms that contribute to bone metastasis and allow for preclinical testing of therapies to prevent and treat tumor metastasis to bone. Finally as the syngeneic tumor cells are injected into immunocompetent mice, this model will provide a means to study interactions between the immune system, tumors and bone, and therapies that target such interactions.
authors: Oncology Research Centre, Prince of Wales Hospital, Randwick, NSW, Australia. c.power@unsw.edu.au
authors: Carl A
authors: CA
authors: Power
authors: Hnin
authors: H
authors: Pwint
authors: Jeffrey
authors: J
authors: Chan
authors: Jae
authors: J
authors: Cho
authors: Yan
authors: Y
authors: Yu
authors: William
authors: W
authors: Walsh
authors: Pamela J
authors: PJ
authors: Russell
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D001859:Bone Neoplasms / Q000097:blood / Q000276:immunology / Q000556:secondary*; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D004195:Disease Models, Animal; D006651:Histocytochemistry; D007121:Immunocompetence; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D015675:Osteocalcin / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000276:immunology / Q000473:pathology*; D014057:Tomography, X-Ray Computed
chemical_list: D015675:Osteocalcin; D000135:Acid Phosphatase