Comprehensive seroprofiling of sixteen B. burgdorferi OspC: implications for Lyme disease diagnostics design.
Early diagnosis of Lyme disease (LD) is critical to successful treatment. However, current serodiagnostic tests do not reliably detect antibodies during early infection. OspC induces a potent early immune response and is also one of the most diverse proteins in the Borrelia proteome. Yet, at least 70% of the amino acid sequence is conserved among all 21 known OspC types. We performed a series of comprehensive seroprofiling studies to select the OspC types that have the most cross-reactive immunodominant epitopes. We found that proteins belonging to seven OspC types detect antibodies from all three infected host species regardless of the OspC genotype of the infecting strain. Although no one OspC type identifies all seropositive human samples, combinations of as few as two OspC proteins identified all patients that had anti-OspC antibodies.
Ivanova
Larisa
L
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Christova
Iva
I
Neves
Vera
V
Aroso
Miguel
M
Meirelles
Luciana
L
Brisson
Dustin
D
Gomes-Solecki
Maria
M
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004285:Dogs; D005838:Genotype; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010542:Peromyscus; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C079616:OspC protein; D011994:Recombinant Proteins