Enhancing the multiplication of nucleopolyhedrovirus in vitro by manipulation of the pH.
Insect nucleopolyhedroviruses (NPVs) are studied widely as agents for biological control, as expression vectors for the production of heterologous proteins, and as transduction vectors for gene therapy applications. Most of these applications rely on the existence of cell lines that allow in vitro multiplication of the virus. The influence of pH in the medium culture on the multiplication of SeMNPV, HearSNPV and AcMNPV in different cell culture lines was investigated. The study showed a strong influence of the medium pH on the virus multiplication with the best results at pH 6.5, about half pH unit above the pH of insect culture media used most commonly. Additional experiments using a recombinant AcMNPV, expressing the green fluorescent protein, suggested that the enhanced virus multiplication at pH 6.5 is due mainly to a facilitated entry of the budded virions into the cells.
Jakubowska
Agata
A
Department of Genetics, Universitat de València, Burjassot (Valencia), Spain.
Ferré
Juan
J
Herrero
Salvador
S
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D017924:Nucleopolyhedroviruses / Q000502:physiology* / Q000648:ultrastructure; D018411:Spodoptera / Q000821:virology*; D053586:Virus Internalization; D014779:Virus Replication
D003470:Culture Media; D049452:Green Fluorescent Proteins