title: Ionic-complementary peptide matrix for enzyme immobilization and biomolecular sensing.
abstract: A novel electrochemical biosensing platform is described using biocompatible, self-assembled ionic-complementary peptide nanofibers. The compatibility of a graphite electrode modified by these peptide nanofibers with enzymes is demonstrated using a model enzyme glucose oxidase (GOx). A glucose biosensor has been successfully fabricated by incorporating this enzyme into the modified electrode. From measurement of its electrode response and sensitivity, this nanofiber-modified electrode shows promise as an enzyme-based biosensor. The findings presented here demonstrate excellent potential of the use of ionic-complementary peptides to modify electrode surfaces for biomolecular sensing and diagnostics.
authors: Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
authors: Hong
authors: H
authors: Yang
authors: Shan-Yu
authors: SY
authors: Fung
authors: Mark
authors: M
authors: Pritzker
authors: P
authors: P
authors: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008962:Models, Theoretical; D010455:Peptides / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D010455:Peptides