title: Novel Golgi protein, GoPro49, is a specific dental follicle marker.
abstract: GoPro49 is a recently identified, novel Golgi protein that is expressed in embryonic mesenchymal tissues, including dental follicle. In the present study, we have tested the hypothesis that the gene is a specific marker for the dental follicle, and examined its expression during the development of mouse incisors and molars. In situ hybridization showed that GoPro49 is expressed in dental follicles from bud to post-eruption stages. The expression is intense throughout the dental follicle during crown development, and persists in the root follicle during root development. In the forming periodontal ligament, GoPro49 expression is down-regulated upon differentiation of the follicle cells to cementoblasts and osteoblasts marked by Bsp1. In cultured dental follicle cells, the GoPro49 protein co-localizes with beta-COP, suggesting that GoPro49 may function in the secretory pathway. We conclude that GoPro49 is a novel, specific marker for the dental follicle and can be used to identify this tissue.
  
  
  BACKGROUND
  Bsp1, bone sialoprotein 1; GoPro49, Golgi protein 49 kDa; E16, embryonic day 16; HERS, Hertwig's epithelial root sheath; PDL, periodontal ligament; dpn, day post-natal.
authors: University of Helsinki, Department of Biological and Environmental Sciences, Division of Biochemistry, P.O. Box 56 (Viikinkaari 5D), University of Helsinki FIN-00014, Finland.
authors: M S
authors: MS
authors: Takatalo
authors: M
authors: M
authors: Tummers
authors: I
authors: I
authors: Thesleff
authors: R
authors: R
authors: Rönnholm
mesh_terms: D000818:Animals; D015415:Biomarkers; D002478:Cells, Cultured; D020756:Coatomer Protein / Q000096:biosynthesis; D003795:Dental Sac / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D006056:Golgi Apparatus / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009805:Odontogenesis / Q000235:genetics; D010513:Periodontal Ligament / Q000254:growth & development
chemical_list: D015415:Biomarkers; D020756:Coatomer Protein; C532315:GoPro49 protein, mouse; D008565:Membrane Proteins