title: Folic acid supplementation modifies beta-adrenoceptor-mediated in vitro lipolysis of obese/diabetic (+db/+db) mice.
abstract: The effects of folic acid (5.7 and 71 microg/kg, 4 weeks) consumption on the beta-adrenoceptors (beta-ARs)-elicited lipolysis in vitro of the abdominal adipocytes of lean/control (+m/+db) and obese/diabetic (+db/+db) mice (female) were investigated. beta-AR agonists (salbutamol, a beta(2)-AR agonist; BRL 37344 and CGP 12177, beta(3)-AR agonists; adrenaline, a beta-AR agonist)-mediated lipolysis, beta(2)-, and beta(3)-ARs protein expression of the adipose tissues after folic acid consumption were evaluated. Our results demonstrate that a smaller magnitude of the basal (spontaneous) and the beta-AR agonists-triggered lipolysis was observed in +db/+db mice, and folic acid supplementation (71 microg/kg) resulted in an improvement of both the baseline and the beta-ARs-mediated lipolysis. In controls, a lower beta(2)-and beta(3)-ARs protein expression of the adipose tissues was detected in +db/+db mice, compared to +m/+db mice. In both strains fed with folic acid (71 microg/kg), a reduction of beta(2)-AR protein expression was observed compared to the respective controls. In +db/+db mice, folic acid (5.7 and 71 microg/kg) consumption caused a dose-dependent increase of beta(3)-AR protein expression compared to controls. We demonstrate that lipolysis elicited by beta-AR (beta(2)- and beta(3)-ARs) agonists was blunted in +db/+db mice. Folic acid consumption has significant modulatory effects on beta-ARs protein expression and lipolysis.
authors: Li Ka Shing Institute of Health Sciences, University of Hong Kong, Shatin, N.T., Hong Kong, PR China.
authors: Tsz Yan
authors: TY
authors: Lam
authors: Sai Wang
authors: SW
authors: Seto
authors: Alice Lai Shan
authors: AL
authors: Au
authors: Christina Chui Wa
authors: CC
authors: Poon
authors: Rachel Wai Sum
authors: RW
authors: Li
authors: Ho Yeung
authors: HY
authors: Lam
authors: Wing Sze
authors: WS
authors: Lau
authors: Shun Wan
authors: SW
authors: Chan
authors: Sai Ming
authors: SM
authors: Ngai
authors: George Pak Heng
authors: GP
authors: Leung
authors: Simon Ming Yuen
authors: SM
authors: Lee
authors: Stephen Kwok Wing
authors: SK
authors: Tsui
authors: Yiu Wa
authors: YW
authors: Kwan
mesh_terms: D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005492:Folic Acid / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D011941:Receptors, Adrenergic / Q000187:drug effects*; D054411:Receptors, Leptin / Q000235:genetics*; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D011941:Receptors, Adrenergic; D054411:Receptors, Leptin; D014815:Vitamins; C515447:leptin receptor, mouse; D005492:Folic Acid