title: Expression of matrix metalloproteinase-1, matrix metalloproteinase-2 and extracellular metalloproteinase inducer in human periodontal ligament cells stimulated with interleukin-1 beta.
abstract: OBJECTIVE
  Matrix metalloproteinases (MMPs), produced by both infiltrating and resident cells of the periodontium, play important roles in physiologic and pathologic events. Both interleukin-1 beta and extracellular MMP inducer can stimulate the expression of MMPs, which in turn leads to breakdown of the periodontium. However, it is currently unknown whether interleukin-1 beta up-regulates MMPs through stimulating the expression of extracellular MMP inducer. The aims of this study were to investigate the effect of interleukin-1 beta on the expression of MMP-1, MMP-2 and extracellular MMP inducer in human periodontal ligament cells and to evaluate whether the regulation of MMP-1 and MMP-2 by this cytokine occurred through an effect on extracellular MMP inducer expression.
  
  
  METHODS
  Cultured human periodontal ligament cells were treated with varying concentrations (0.01-10 ng/mL) of interleukin-1 beta at for 6, 12 and 24 h. Reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, gelatin zymography and western blotting were performed to measure the mRNA and protein levels of MMP-1, MMP-2 and extracellular MMP inducer.
  
  
  RESULTS
  Basal levels of mRNA and protein for MMP-1, MMP-2 and extracellular MMP inducer were detected in untreated human periodontal ligament cells. Interleukin-1 beta significantly up-regulated the expression of MMP-1 and MMP-2 mRNA and protein (p < 0.05); however, the levels of mRNA and protein for extracellular MMP inducer were not significantly different (p > 0.05). In the culture medium, the concentration of MMP-1 was also increased significantly, but the concentration of MMP-1 was not related to the concentration of extracellular MMP inducer (R(2) = 0.2538, p > 0.05).
  
  
  CONCLUSIONS
  Interleukin-1 beta up-regulated the levels of MMP-1 and MMP-2, but it did not alter the expression of extracellular MMP inducer. Expression of MMP-1 and MMP-2 might be elevated by interleukin-1 beta and extracellular MMP inducer via two different signal pathways.
authors: Key Laboratory for Oral Biomedical Engineering of the Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: J
authors: J
authors: Xiang
authors: C
authors: C
authors: Li
authors: W
authors: W
authors: Dong
authors: Z
authors: Z
authors: Cao
authors: L
authors: L
authors: Liu
mesh_terms: D051926:Basigin / Q000032:analysis / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006801:Humans; D053583:Interleukin-1beta / Q000008:administration & dosage / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1 / Q000032:analysis / Q000187:drug effects*; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D003470:Culture Media; D053583:Interleukin-1beta; D012333:RNA, Messenger; D051926:Basigin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1