title: Significance of four MRD markers in MRD-based treatment strategy for childhood acute lymphoblastic leukemia.
abstract: Newly diagnosed children with ALL (n=32) were treated on a protocol incorporating minimal residual disease (MRD)-based treatment decisions. MRD was monitored at 4 time points by semi-quantitative PCR detection of antigen receptor gene rearrangement, flow cytometry, quantitative RT-PCR detection of chimeric gene transcripts and overexpressed WT1 mRNA. Four patients positive for MRD at week 5 were treated with an intensified regimen. Median follow-up was 5.0 years (range 3.8-6.6 years) with a 4-year event-free survival rate of 93.8+/-4.3%. This MRD-based treatment strategy seems to be highly successful and may improve the outcomes of children with ALL. A large study is warranted.
authors: Department of Hematology/Oncology, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo, Izumi City, Osaka 594-1101, Japan. asawada@mch.pref.osaka.jp
authors: Akihisa
authors: A
authors: Sawada
authors: Naoki
authors: N
authors: Sakata
authors: Tomoko
authors: T
authors: Kishimoto
authors: Banryoku
authors: B
authors: Higuchi
authors: Maho
authors: M
authors: Koyama
authors: Osamu
authors: O
authors: Kondo
authors: Emiko
authors: E
authors: Sato
authors: Takayuki
authors: T
authors: Okamura
authors: Masahiro
authors: M
authors: Yasui
authors: Masami
authors: M
authors: Inoue
authors: Akira
authors: A
authors: Yoshioka
authors: Keisei
authors: K
authors: Kawa
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007223:Infant; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000188:drug therapy / Q000628:therapy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor