Focal modulation microscopy.
We report a novel light microscopy method for high resolution molecular imaging of thick biological tissues with one photon excited fluorescence. Effective optical sectioning and diffraction limited spatial resolution are achieved when imaging deep inside a multiple-scattering medium by the use of focal modulation, a technique for suppressing the background fluorescence signal excited by scattered light. Our method has been validated with animal tissue and an imaging depth around 600 microns has been demonstrated.
Chen
Nanguang
N
Division of Bioengineering, National University of Singapore, Singapore. biecng@nus.edu.sg
Wong
Chee-Howe
CH
Sheppard
Colin J R
CJ
D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity