Study on docetaxel-loaded nanoparticles with high antitumor efficacy against malignant melanoma.
Docetaxel (Doc) has extraordinary activities against a variety of solid tumors. However, the clinical efficacy of Doc is limited due to its poor solubility, low selective distribution, fast elimination in vivo, etc. In the present study, Doc was incorporated into the core-shell structure of nanoparticles prepared based on our previous work. The obtained docetaxel-loaded nanoparticles (DOCNP) were characterized with various biophysical methodologies, and its antitumor efficacy against malignant melanoma was evaluated both in vitro and in vivo. Our results indicated that Doc could be incorporated into the nanoparticles with high encapsulation efficiency (>90%). The incorporated Doc can be released from DOCNP in a sustained manner. In vitro cytotoxicity studies indicated that DOCNP could effectively kill B16 cells and show a dose- and time-dependent efficacy. Furthermore, intratumoral administration revealed that DOCNP has significantly higher antitumor effect and lower toxicity to normal cells and tissues than free Doc. These results suggest that DOCNP may be a promising drug delivery system in therapy for malignant melanoma.
Zheng
Donghui
D
Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Li
Xiaolin
X
Xu
Huae
H
Lu
Xiaowei
X
Hu
Yong
Y
Fan
Weixin
W
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000077143:Docetaxel; D016503:Drug Delivery Systems; D008297:Male; D008545:Melanoma / Q000473:pathology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000648:ultrastructure; D043823:Taxoids / Q000008:administration & dosage* / Q000494:pharmacology
D000970:Antineoplastic Agents; D043823:Taxoids; D000077143:Docetaxel