title: Definition of purified enzyme-linked immunosorbent assay antigens from the culture filtrate protein of Mycobacterium bovis by proteomic analysis.
abstract: Enzyme-linked immunosorbent assay (ELISA) has been developed as the ancillary diagnosis of bovine tuberculosis at ante-mortem to overcome the disadvantages of intradermal skin test. In this study, the antigenic proteins were purified, applied to bTB ELISA, and identified through proteomic analysis. Culture filtrate protein of Mycobacterium bovis was fractionated by MonoQ column chromatography, and examined the antigenicity by immunoblotting. The antigenic 20 kDa protein was in-gel digested and identified the antigenome by LTQ mass spectrometer and peptide match fingerprinting, which were MPB64, MPB70, MPB83, Fas, Smc, Nrp, RpoC, Transposase, LeuA, and MtbE. The 20 kDa protein exhibited the highest antigenicity to bTB positive cattle in ELISA and would be useful for bTB serological diagnosis.
authors: Bacteriology and Parasitology Division, National Veterinary Research Institute, National Veterinary Research & Quarantine Service, Anyang, Kyounggi, Republic of Korea. choys@nvrqs.go.kr
authors: Yun Sang
authors: YS
authors: Cho
authors: Sang-Eun
authors: SE
authors: Lee
authors: Young Joon
authors: YJ
authors: Ko
authors: Donghee
authors: D
authors: Cho
authors: Hyang Shim
authors: HS
authors: Lee
authors: Inyeong
authors: I
authors: Hwang
authors: Hyangmi
authors: H
authors: Nam
authors: Eunjung
authors: E
authors: Heo
authors: Jong Man
authors: JM
authors: Kim
authors: Sukchan
authors: S
authors: Jung
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary*; D009163:Mycobacterium bovis / Q000276:immunology*; D010449:Peptide Mapping / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins