Development of a rapid and sensitive magnetic chemiluminescent enzyme immunoassay for detection of luteinizing hormone in human serum.
OBJECTIVE
A rapid and sensitive magnetic particles (MPs)-based chemiluminescence enzyme immunoassay (MPs-CLEIA) for determination of luteinizing hormone (LH) in human serum was developed and validated.


METHODS
The method was developed based on a sandwiched immunoreaction format, in which MPs served as both the solid phase and separator. The horseradish peroxidase(HRP)-luminol-H(2)O(2) chemiluminescent system was chosen as the detection system.


RESULTS
This method had a detection limit of 0.2 mIU/mL and a linear range of 0.5-200 mIU/mL. The assay showed little cross-reactivity, and the intra- and inter-assay coefficients of variation were less than 10%. The recoveries were from 101.6% to 104.1%. The method has been successfully applied to detection of LH in human serum and showed good correlation compared with radioimmunoassay (RIA).


CONCLUSIONS
The MPs-CLEIA provided apparent advantages over RIA, and facilitated the development of high-throughput screening and automated operation systems in clinical diagnosis.
Xiao
Qin
Q
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Li
Haifang
H
Hu
Guomao
G
Wang
Hongrui
H
Li
Zhenjia
Z
Lin
Jin-Ming
JM
D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D049449:Luminescence; D008165:Luminol / Q000737:chemistry; D007986:Luteinizing Hormone / Q000097:blood* / Q000737:chemistry; D008280:Magnetics; D015203:Reproducibility of Results
D008165:Luminol; D007986:Luteinizing Hormone; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase