Effect of protein solution components in the adsorption of Herbaspirillum seropedicae GlnB protein on mica.
The adsorption of proteins and its buffer solution on mica surfaces was investigated by atomic force microscopy (AFM). Different salt concentration of the Herbaspirillum seropedicae GlnB protein (GlnB-Hs) solution deposited on mica was investigated. This protein is a globular, soluble homotrimer (36kDa), member of PII-like proteins family involved in signal transducing in prokaryote. Supramolecular structures were formed when this protein was deposited onto bare mica surface. The topographic AFM images of the GlnB-Hs films showed that at high salt concentration the supramolecular structures are spherical-like, instead of the typical doughnut-like shape for low salt concentration. AFM images of NaCl and Tris from the buffer solution showed structures with the same pattern as those observed for high salt protein solution, misleading the image interpretation. XPS experiments showed that GlnB protein film covers the mica surface without chemical reaction.
Ferreira
Cecília F G
CF
PIPE - Programa de Pós-Graduação em Engenharia, Universidade Federal do Paraná - UFPR, Curitiba, Brazil. sandraferreira2001@yahoo.com.br
Benelli
Elaine M
EM
Klein
Jorge J
JJ
Schreiner
Wido
W
Camargo
Paulo C
PC
D000327:Adsorption; D000538:Aluminum Silicates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism* / Q000648:ultrastructure; D018441:Biofilms; D042803:Herbaspirillum / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D051922:PII Nitrogen Regulatory Proteins / Q000378:metabolism* / Q000648:ultrastructure; D012996:Solutions; D013057:Spectrum Analysis; D013499:Surface Properties
D000538:Aluminum Silicates; D001426:Bacterial Proteins; D051922:PII Nitrogen Regulatory Proteins; D012996:Solutions; C404143:PIID regulatory protein, Bacteria; C011934:mica