title: A novel glucose biosensor based on the immobilization of glucose oxidase onto gold nanoparticles-modified Pb nanowires.
abstract: A novel glucose biosensor was developed, based on the immobilization of glucose oxidase (GOD) with cross-linking in the matrix of bovine serum albumin (BSA) on a Pt electrode, which was modified with gold nanoparticles decorated Pb nanowires (GNPs-Pb NWs). Pb nanowires (Pb NWs) were synthesized by an L-cysteine-assisted self-assembly route, and then gold nanoparticles (GNPs) were attached onto the nanowire surface through -SH-Au specific interaction. The morphological characterization of GNPs-Pb NWs was examined by transmission electron microscopy (TEM). Cyclic voltammetry and chronoamperometry were used to study and to optimize the electrochemical performance of the resulting biosensor. The synergistic effect of Pb NWs and GNPs made the biosensor exhibit excellent electrocatalytic activity and good response performance to glucose. The effects of pH and applied potential on the amperometric response of the biosensor have been systemically studied. In pH 7.0, the biosensor showed the sensitivity of 135.5 microA mM(-1) cm(-2), the detection limit of 2 microM (S/N=3), and the response time <5 s with a linear range of 5-2200 microM. Furthermore, the biosensor exhibits good reproducibility, long-term stability and relative good anti-interference.
authors: The Key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors: Huicai
authors: H
authors: Wang
authors: Xinsheng
authors: X
authors: Wang
authors: Xiaoqing
authors: X
authors: Zhang
authors: Xia
authors: X
authors: Qin
authors: Zixia
authors: Z
authors: Zhao
authors: Zhiying
authors: Z
authors: Miao
authors: Nan
authors: N
authors: Huang
authors: Qiang
authors: Q
authors: Chen
mesh_terms: D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D012710:Serum Albumin, Bovine; D007854:Lead; D010984:Platinum; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose