[Combination of short-period sperm-oocyte coincubation and early rescue intracytoplasmic sperm injection after total failure of in vitro fertilization].
OBJECTIVE
To evaluate the outcome of the combination of short-period sperm-oocyte coincubation with early rescue intracytoplasmic sperm injection (ICSI) after complete failure of in vitro fertilization (IVF).


METHODS
We used short-period sperm-oocyte coincubation and overnight fertilization for the oocytes obtained from 10 IVF-ET cycles, and compared the fertilization results and embryo quality. We conducted polar body observation following short-period sperm-oocyte coincubation for 105 conventional IVF cycles, and evaluated the clinical outcome of early rescue ICSI for total fertilization failure.


RESULTS
No significant differences were noted in fertilization outcome and embryo quality between the 3 h short-period and overnight fertilizations (P > 0.05). The combined strategy achieved a clinical pregnancy rate of 53.3%, an implantation rate of 38.0%, with 64 babies born, of whom 44.8% were taken home.


CONCLUSIONS
The combination of short-period sperm-oocyte coincubation with early rescue ICSI can significantly improve the clinical outcome in patients who have experienced complete failure of in vitro fertilization.
Zhang
Ning-yuan
NY
Reproductive Medicine Center, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China.
Sun
Hai-xiang
HX
Hu
Ya-li
YL
Wang
Bin
B
Xu
Zhi-peng
ZP
D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility / Q000628:therapy*; D008297:Male; D010063:Ovum; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D017211:Treatment Failure