Preliminary structural characterization of human SOUL, a haem-binding protein.
Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP(23) protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6(4)22, with unit-cell parameters a = b = 145, c = 60 A and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 A resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.
Freire
Filipe
F
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Romão
Maria João
MJ
Macedo
Anjos L
AL
Aveiro
Susana S
SS
Goodfellow
Brian J
BJ
Carvalho
Ana Luísa
AL
D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000737:chemistry*; D006801:Humans; D011257:Pregnancy Proteins / Q000737:chemistry*
D002352:Carrier Proteins; C000631029:HEBP1 protein, human; C516258:HEBP2 protein, human; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D011257:Pregnancy Proteins