[Nav1.8 and Nav1.9 mRNA expression in rat trigeminal ganglion at different interval after molar extraction].
OBJECTIVE
To observe the expression and function of extraction.


METHODS
Real-time reverse transcription PCR paralleled with vitro-established cRNA standard curves was applied to measure the expression of Nav1.8, Nav1.9 at 30 min, 2 h, 1 d, 3 d and 6 d respectively after extraction of rat right mandibular molars. The right mandibular molars were used as control.


RESULTS
Both Nav1.8 and Nav1.9 mRNA in right trigeminal ganglion showed little change after 30 min, and increased slowly after 2 h. Nav1.8, Nav1.9 mRNA expressions increased by 27% and 24.5% respectively compared to the left trigeminal ganglion after 3 d, reaching the highest level (P < 0.05), and then the expressions began decreasing from 6 d.


CONCLUSIONS
The pain caused by molar extraction is related to the up-regulation of expressions of sodium channels protein Nav1.8 and Nav1.9 mRNA, indicating the participation of sodium channels in regulations of peripheral tissue pain after molar extraction.
Zhang
Lei
L
Stomatological Center, General Hospital of PLA, Beijing 100853, China.
Liu
Hong-Chen
HC
Wang
Dong-Sheng
DS
D000818:Animals; D008297:Male; D062559:NAV1.8 Voltage-Gated Sodium Channel; D062558:NAV1.9 Voltage-Gated Sodium Channel; D009479:Neuropeptides / Q000378:metabolism*; D010149:Postoperative Pain / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000378:metabolism*; D014081:Tooth Extraction; D012668:Trigeminal Ganglion / Q000378:metabolism*
D062559:NAV1.8 Voltage-Gated Sodium Channel; D062558:NAV1.9 Voltage-Gated Sodium Channel; D009479:Neuropeptides; D012333:RNA, Messenger; C568319:Scn10a protein, rat; C113705:Scn11a protein, rat; D015222:Sodium Channels