Democratization and integration of genomic profiling tools.
Systems biology is a comprehensive means of creating a complete understanding of how all components of an organism work together to maintain and procreate life. By quantitatively profiling one at a time, the effect of thousands and millions of genetic and environmental perturbations on the cell, systems biologists are attempting to recreate and measure the effect of the many different states that have been explored during the 3 billion years in which life has evolved. A key aspect of this work is the development of innovative new approaches to quantify changes in the transcriptome, proteome, and metabolome. In this chapter we provide a review and evaluation of several genomic profiling techniques used in plant systems biology as well as make recommendations for future progress in their use and integration.
Sussman
Michael R
MR
Department of Biochemistry, UW Biotechnology Center, University of Wisconsin-Madison, Madison, WI, USA.
Huttlin
Edward L
EL
Wohlbach
Dana J
DJ
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics / Q000379:methods*; D055442:Metabolome; D020543:Proteome / Q000032:analysis; D017150:Public Sector; D012984:Software / Q000600:supply & distribution*; D049490:Systems Biology / Q000379:methods
D020543:Proteome