title: Phylogenetic distribution of virulence genes in Escherichia coli isolated from bovine mastitis in Iran.
abstract: One hundred and twenty seven Escherichia coli isolates from bovine mastitis were examined to detect the phylogenetic group/subgroups and a selection of virulence associated genes. Forty nine (38.58%) isolates belonged to group B1 the remaining isolates fell into four phylogenetic subgroups: A(0) (18.11%), A(1) (26.77%), D(1) (6.29%) and D(2) (10.23%). None of the isolates belonged to B2 group. Forty seven (37.00%) isolates were positive for at least one virulence gene, among them f17A was the most common gene, found in 20.47% of the isolates. Among the E. coli isolates, 11.81% had iucD, 9.44% f17c-A, 9.44% cnf2, 7.87% f17b-A, 6.29% afaD-8 and afaE-8, 3.14% f17d-A, 0.78% cnf1 and 0.78% clpG genes. All of the detected virulence genes were present alone or in combination with each other except clpG and f17d-A genes that were only found alone. None of the isolates contained the genes for F17a-A, intimin, P or S fimbriae.
authors: Department of Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, 14111 Kerman, Iran. Ghanbar@mail.uk.ac.ir
authors: Reza
authors: R
authors: Ghanbarpour
authors: Eric
authors: E
authors: Oswald
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D007492:Iran; D008414:Mastitis, Bovine / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction