Neuroprotection from diazinon-induced toxicity in differentiating murine N2a neuroblastoma cells.
In previous work, the outgrowth of axon-like processes by differentiating mouse N2a neuroblastoma cells was shown to be inhibited by exposure to 10 microM diazinon. In the present work, N2a cells were induced to differentiate for 24 h in the presence and absence of 10 microM diazinon and 20% (v/v) conditioned medium derived from differentiating rat C6 glioma cells. Cells were then stained or lysed for morphological and biochemical analyses, respectively. The data showed that co-treatment with conditioned medium prevented the neurite inhibitory effect of diazinon. Furthermore, a significant recovery was also observed in the reduced levels of neurofilament heavy chain (NFH), heat shock protein-70 (HSP-70) and growth-associated protein-43 (GAP-43) observed as a result of diazinon treatment in the absence of conditioned medium, as seen by densitometric analysis of Western blots of cell lysates probed with monoclonal antibodies N52, BRM-22 and GAP-7B10. By contrast, no significant change was noted in the reactivity of cell lysates with antibodies against alpha- and beta-tubulin under any condition tested. After pre-incubation with a polyclonal anti-glial cell line-derived neurotrophic factor (GDNF) antibody, conditioned medium derived from rat C6 glioma cells lost its ability to protect N2a cells against the neurite inhibitory effects of diazinon. In conclusion, these data demonstrate that C6 conditioned medium protects N2a cells from the neurite inhibitory effects of diazinon by blocking molecular events leading to axon damage and that GDNF is implicated in these effects.
Harris
Wayne
W
School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Sachana
Magda
M
Flaskos
John
J
Hargreaves
Alan J
AJ
D000704:Analysis of Variance; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001369:Axons / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003976:Diazinon / Q000494:pharmacology*; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000276:immunology; D005910:Glioma / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism
D000906:Antibodies; D002800:Cholinesterase Inhibitors; D017077:Culture Media, Conditioned; D019922:GAP-43 Protein; D051100:Glial Cell Line-Derived Neurotrophic Factor; D018840:HSP70 Heat-Shock Proteins; D016900:Neurofilament Proteins; C051774:neurofilament protein H; D003976:Diazinon