title: Identification of neural programmed cell death through the detection of DNA fragmentation in situ and by PCR.
abstract: Programmed cell death is a fundamental process for the development and somatic maintenance of organisms. This unit describes methods for visualizing both dying cells in situ and for detection of nucleosomal ladders. A description of various current detection strategies is provided, as well as support protocols for preparing positive and negative controls and for preparing genomic DNA.
authors: Helen L. Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The Scripps Research Institute, La Jolla, California, USA.
authors: Yun C
authors: YC
authors: Yung
authors: Grace
authors: G
authors: Kennedy
authors: Jerold
authors: J
authors: Chun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053938:DNA Fragmentation; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D020287:In Situ Nick-End Labeling / Q000379:methods*; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods*