title: The histone gene activator HINFP is a nonredundant cyclin E/CDK2 effector during early embryonic cell cycles.
abstract: Competency for DNA replication is functionally coupled to the activation of histone gene expression at the onset of S phase to form chromatin. Human histone nuclear factor P (HiNF-P; gene symbol HINFP) bound to its cyclin E/cyclin-dependent kinase 2 (CDK2) responsive coactivator p220(NPAT) is a key regulator of multiple human histone H4 genes that encode a major subunit of the nucleosome. Induction of the histone H4 transcription factor (HINFP)/p220(NPAT) coactivation complex occurs in parallel with the CDK-dependent release of pRB from E2F at the restriction point. Here, we show that the downstream CDK-dependent cell cycle effector HINFP is genetically required and, in contrast to the CDK2/cyclin E complex, cannot be compensated. We constructed a mouse Hinfp-null mutation and found that heterozygous Hinfp mice survive, indicating that 1 allele suffices for embryogenesis. Homozygous loss-of-function causes embryonic lethality: No homozygous Hinfp-null mice are obtained at or beyond embryonic day (E) 6.5. In blastocyst cultures, Hinfp-null embryos exhibit a delay in hatching, abnormal growth, and loss of histone H4 gene expression. Our data indicate that the CDK2/cyclin E/p220(NPAT)/HINFP/histone gene signaling pathway at the G1/S phase transition is an essential, nonredundant cell cycle regulatory mechanism that is established early in embryogenesis.
authors: Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA.
authors: Ronglin
authors: R
authors: Xie
authors: Ricardo
authors: R
authors: Medina
authors: Ying
authors: Y
authors: Zhang
authors: Sadiq
authors: S
authors: Hussain
authors: Jennifer
authors: J
authors: Colby
authors: Prachi
authors: P
authors: Ghule
authors: Sakthi
authors: S
authors: Sundararajan
authors: Marilyn
authors: M
authors: Keeler
authors: Li-Jun
authors: LJ
authors: Liu
authors: Margaretha
authors: M
authors: van der Deen
authors: Partha
authors: P
authors: Mitra
authors: Jane B
authors: JB
authors: Lian
authors: Jaime A
authors: JA
authors: Rivera-Perez
authors: Stephen N
authors: SN
authors: Jones
authors: Janet L
authors: JL
authors: Stein
authors: Andre J
authors: AJ
authors: van Wijnen
authors: Gary S
authors: GS
authors: Stein
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D016193:G1 Phase / Q000235:genetics / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013997:Time Factors
chemical_list: D018797:Cell Cycle Proteins; D019927:Cyclin E; C524750:HiNF-P protein, mouse; D006657:Histones; D009687:Nuclear Proteins; D012097:Repressor Proteins; C543824:p220(NPAT) protein, mouse; C495893:Cdk2 protein, mouse; D051357:Cyclin-Dependent Kinase 2