title: Healing of extraction sockets in collagenase-2 (matrix metalloproteinase-8)-deficient mice.
abstract: Matrix metalloproteinase-8 (MMP-8) participates in skin wound healing and inflammation. We hypothesized that MMP-8 plays a role in wound healing after tooth extraction and in periapical inflammation. Bone formation, collagen metabolism, and inflammation in tooth extraction socket and in periapical lesions were analyzed in wild-type mice and in MMP-8-deficient (MMP-8(-/-)) mice. New trabecular bone area in the extraction sockets and in periapical lesions were similar in both groups. In extraction sockets significantly more type III procollagen was synthesized, and the neutrophil and MMP-9 levels were lower in MMP-8(-/-) mice. The amount of Fas ligand, identified as a substrate for MMP-8, was lower in alveolar mucosa but higher in alveolar bone of MMP-8(-/-) mice. These results indicate that MMP-8 can modulate inflammation and collagen metabolism of alveolar bone and mucosa.
authors: Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, Finland.
authors: Jarkko T
authors: JT
authors: Korpi
authors: Pirjo
authors: P
authors: Aström
authors: Niko
authors: N
authors: Lehtonen
authors: Leo
authors: L
authors: Tjäderhane
authors: Soili
authors: S
authors: Kallio-Pulkkinen
authors: Maria
authors: M
authors: Siponen
authors: Timo
authors: T
authors: Sorsa
authors: Emma
authors: E
authors: Pirilä
authors: Tuula
authors: T
authors: Salo
mesh_terms: D000539:Alveolar Process / Q000201:enzymology; D000818:Animals; D054428:Chemokine CX3CL1 / Q000032:analysis; D003094:Collagen / Q000378:metabolism; D024061:Collagen Type III / Q000096:biosynthesis; D053222:Fas Ligand Protein / Q000032:analysis; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D007371:Interferon-gamma / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000172:deficiency*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009061:Mouth Mucosa / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010483:Periapical Diseases / Q000201:enzymology / Q000503:physiopathology; D011347:Procollagen / Q000096:biosynthesis; D014081:Tooth Extraction; D020390:Tooth Socket / Q000201:enzymology* / Q000503:physiopathology; D014945:Wound Healing / Q000502:physiology
chemical_list: D054428:Chemokine CX3CL1; D024061:Collagen Type III; D053222:Fas Ligand Protein; D015850:Interleukin-6; D011347:Procollagen; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003094:Collagen; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse