title: Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy.
abstract: IR absorption of chemical species in microscopic objects such as biological cells cannot be measured by conventional IR microscopes, because of their low resolution. To overcome this problem, we developed a novel far-field IR super-resolution microscope employing transient fluorescence detected IR spectroscopy. The resolution of this microscope was shown to be 880 nm by measuring the image of 1 microm fluorescent beads. Furthermore, it succeeded in resolving beads located 1.4 microm apart from each other. This is considerably smaller than the diffraction limit of the applied IR light (3.4 microm). These results suggest the capability of our microscope to study sub-micron targets such as sub-cellular structures of biological cells.
authors: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Yokohama 226-8503, Japan.
authors: Keiichi
authors: K
authors: Inoue
authors: Nándor
authors: N
authors: Bokor
authors: Satoshi
authors: S
authors: Kogure
authors: Masaaki
authors: M
authors: Fujii
authors: Makoto
authors: M
authors: Sakai
mesh_terms: D003584:Cytological Techniques; D007091:Image Processing, Computer-Assisted; D007259:Infrared Rays; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D055095:Optics and Photonics; D013997:Time Factors