title: Antimicrobial peptide interactions with silica bead supported bilayers and E. coli: buforin II, magainin II, and arenicin.
abstract: Using the unique quantitative capabilities of hyperspectral confocal microscopy combined with multivariate curve resolution, a comparative approach was employed to gain a deeper understanding of the different types of interactions of antimicrobial peptides (AMPs) with biological membranes and cellular compartments. This approach allowed direct comparison of the dynamics and local effects of buforin II, magainin II, and arenicin with nanoporous silica bead supported bilayers and living E. coli. Correlating between experiments and comparing these responses have yielded several important discoveries for pursuing the underlying biophysics of bacteriocidal specificity and the connection between structure and function in various cellular environments. First, a novel fluorescence method for direct comparison of a model and living system is demonstrated by utilizing the membrane partitioning and environmental sensitivity of propidium iodide. Second, measurements are presented comparing the temporal dynamics and local equilibrium concentrations of the different antimicrobial agents in the membrane and internal matrix of the described systems. Finally, we discuss how the data lead to a deeper understanding of the roles of membrane penetration and permeabilization in the action of these AMPs.
authors: Electronic and Nanostructured Materials, Sandia National Laboratories, Albuquerque, NM 87185-0892, USA. rwdavis@sandia.gov
authors: Ryan W
authors: RW
authors: Davis
authors: Dulce C
authors: DC
authors: Arango
authors: Howland D T
authors: HD
authors: Jones
authors: Mark H
authors: MH
authors: Van Benthem
authors: David M
authors: DM
authors: Haaland
authors: Susan M
authors: SM
authors: Brozik
authors: Michael B
authors: MB
authors: Sinclair
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D054806:Magainins / Q000737:chemistry* / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry* / Q000494:pharmacology; D012822:Silicon Dioxide
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; D008081:Liposomes; D054806:Magainins; D011506:Proteins; C097849:buforin II; D012822:Silicon Dioxide