title: Gradient HPLC separation of dehydroepiandrosterone (DHEA) from its metabolites and biological congeners: role of tetrahydrofuran in the chromatographic mechanism.
abstract: A three-step gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the separation of dehydroepiandrosterone (DHEA), its sulfate ester (DHEA-S), its three C7-oxidized metabolites (7alphaOH-DHEA, 7betaOH-DHEA, 7-keto-DHEA), and its biosynthetic congeners (androstenedione, testosterone, estradiol, pregnenolone). This new method allows the quantitative characterization of DHEA metabolism and biosynthetic transformation under given physiological, pathological, or therapeutically influenced circumstances. Tetrahydrofuran probably acts as a proton acceptor coadsorbent, while isopropanol behaves as a proton donor during the separation of testosterone, estradiol, and the stereoisomers of 7-OH-DHEA.
authors: Department of Pharmaceutical Chemistry, Semmelweis University, Hogyes Endre u. 9, Budapest 1092, Hungary.
authors: András
authors: A
authors: Gergely
authors: Péter
authors: P
authors: Horváth
authors: György
authors: G
authors: Szász
authors: Gábor
authors: G
authors: Veress
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003687:Dehydroepiandrosterone / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism*; D005663:Furans; D012680:Sensitivity and Specificity
chemical_list: D005663:Furans; C018674:tetrahydrofuran; D003687:Dehydroepiandrosterone