title: Biodegradation of a dental resin material by fibroblast conditioned media.
abstract: OBJECTIVE
  Hydrolytic activity is increased in conditioned media from human gingival and pulp fibroblasts in response to exposure to triethyleneglycol dimethacrylate (TEGDMA). The purpose of this study was to determine if this conditioned media with hydrolytic activity could cause the biodegradation of a dental resin material.
  
  
  METHODS
  Resin material specimens were stored for 30 days at 37 degrees C in distilled water, unconditioned media, artificial serum, conditioned media from human gingival and pulp fibroblasts and conditioned media from human pulp and gingival fibroblasts that were exposed to TEGDMA. The media was exchanged every other day. The specimens were subjected to Fourier transform infrared spectroscopy (FTIR) before and after storage. The area under the carbonyl peak was calculated for all the specimens to determine the extent of the degradation.
  
  
  RESULTS
  Differences before and after storage in the area under the carbonyl peak were statistically significant for the specimens stored in the conditioned media from the fibroblasts that were exposed to TEGDMA. All of the other specimens did not produce differences in the area under the carbonyl peak that were statistically significant when comparing the before and after storage results.
  
  
  CONCLUSIONS
  The biodegradation demonstrated here could contribute to the marginal leaking seen clinically with the use of resin materials in dental restorations.
authors: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, IN, USA. kagregso@iupui.edu
authors: Karen S
authors: KS
authors: Gregson
authors: L
authors: L
authors: Jack Windsor
authors: Jeffrey A
authors: JA
authors: Platt
mesh_terms: D001802:Blood Substitutes / Q000737:chemistry; D002244:Carbon / Q000032:analysis; D002478:Cells, Cultured; D003188:Composite Resins / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D017077:Culture Media, Conditioned / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry*; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008422:Materials Testing; D010100:Oxygen / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D001802:Blood Substitutes; D003188:Composite Resins; D003470:Culture Media; D017077:Culture Media, Conditioned; D003764:Dental Materials; D011109:Polymethacrylic Acids; D014867:Water; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D002244:Carbon; D010100:Oxygen