Cytokine and molecular analyses of intraocular lymphoma.
OBJECTIVE
The authors investigate the efficacy of using the cytokine levels and clonal heavy-chain immunoglobulin (IgH) gene rearrangements in the vitreous as adjunctive tools to diagnose intraocular lymphoma (IOL).


METHODS
The IL-10 and IL-6 levels and IgH gene rearrangements were analyzed in vitreous samples from 8 cases of IOL and in 14 uveitis patients.


RESULTS
The level of IL-10 with an IL-10/IL-6 ratio > 1 was significantly higher in all eyes with IOL. B-cell monoclonality was detected in only 5 of 8 eyes with IOL.


CONCLUSIONS
The measurements of the levels of cytokines are valuable as a reliable biomarker.
Ohta
Kouichi
K
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan. ohta@po.mdu.ac.jp
Sano
Kenji
K
Imai
Hiroki
H
Kikuchi
Takanobu
T
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008163:Luminescent Measurements; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D014605:Uveitis / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism / Q000473:pathology*
D014408:Biomarkers, Tumor; C508609:IL10 protein, human; C508600:IL6 protein, human; D007143:Immunoglobulin Heavy Chains; D015850:Interleukin-6; D016753:Interleukin-10