Stationary phase expression, purification, and characterization of XorKI, a restriction endonuclease from Xanthomonas oryzae pv. oryzae.
An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorKI, was heterologously produced in Escherichia coli by applying the stationary state induction method. The yield was 5.4 mg of XorKI per liter of LB medium. XorKI existed in multiple oligomeric forms as evidenced by gel filtration chromatography. The specific activity of purified XorKI was 323000 units per mg.
Kang
Woo Young
WY
Department of Chemistry and Institute for Chemical Biology, 98 Gunja-Dong, Gwangjin-Gu, Seoul, 143-747, Korea.
Chae
Young Kee
YK
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018929:Cell Culture Techniques; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature; D014974:Xanthomonas / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D015252:Deoxyribonucleases, Type II Site-Specific