title: [The preparation of nano-hydroxyapatite and preliminary observation on its effects on the occlusion of dentinal tubule].
abstract: OBJECTIVE
  To synthesize nano-hydroxyapatite (nano-HA) and evaluate its effect on the occlusion of dentinal tubules in vitro, and therefore provide evidence for the clinical application.
  
  
  METHODS
  (NH(4))(2)HPO(4) and Ca(NO3)2 were used to form nano-HA, which was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrometer (FTIR) and X-ray diffractometer (XRD) respectively. Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups, which were control group, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group, and nano-HA group. After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days, the surface of slab dentin was observed using SEM.
  
  
  RESULTS
  SEM, TEM, FTIR, and XRD tests showed that nano-HA was synthesized successfully. SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group.
  
  
  CONCLUSIONS
  In comparison with CPP-ACP, nano-HA could occlude dentinal tubules more effectively in vitro.
authors: Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
authors: Zhe-Jun
authors: ZJ
authors: Wang
authors: Yue
authors: Y
authors: Sa
authors: Xiao
authors: X
authors: Ma
authors: Yi-Ning
authors: YN
authors: Wang
authors: Tao
authors: T
authors: Jiang
mesh_terms: D002364:Caseins; D003764:Dental Materials / Q000138:chemical synthesis*; D003804:Dentin; D003807:Dentin Sensitivity / Q000628:therapy; D017886:Durapatite / Q000138:chemical synthesis*; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures; D013674:Technology, Dental
chemical_list: D002364:Caseins; D003764:Dental Materials; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D017886:Durapatite