Oral systemic administration of Ankaferd blood stopper has no short-term toxicity in an in vivo rabbit experimental model.
BACKGROUND
Ankaferd blood stopper (ABS) is a standardized herbal extract obtained from 5 different plants. In Turkey, it has been approved for local topical applications in external postsurgical and postdental surgery bleedings. Ankaferd blood stopper, besides its hemostatic activity, has in vitro anti-infectious and antineoplastic actions.


OBJECTIVE
The aim of this study was to assess short-term hematological and biochemical safety following the oral systemic administration of ABS to rabbits.


METHODS
Twelve rabbits (aged 6-12 months) were included to test the safety of oral ABS. Animals were divided into 4 groups, which had ABS administered orally at doses of 1, 3, 6, and 9 mL, irrespective of their weight. The general well-being and feeding patterns of the animals were observed for a period of 7 days. Blood samples (5.5 mL) were obtained just before oral administration, on days 1 and 4.


RESULTS
During the observation period of 7 days, none of the animals showed any abnormal behavior or deviation from the normal. Acute mucosal toxicity, hematotoxicity, hepatotoxicity, nephrotoxicity, and biochemical toxicity were not observed during the short-term follow-up of the animals.


CONCLUSIONS
No signs of toxicity were observed in rabbits during short-term study with oral ABS administration.
Bilgili
Hasan
H
Department of SurgeryFaculty of Veterinary Medicine, Ankara UniversityAnkara, Turkey.
Captug
Ozge
O
Kosar
Ali
A
Kurt
Mevlut
M
Kekilli
Murat
M
Shorbagi
Ali
A
Kurt
Ozlem Kar
OK
Ozdemir
Oktay
O
Goker
Hakan
H
Haznedaroglu
Ibrahim Celalettin
IC
D000284:Administration, Oral; D000818:Animals; D004195:Disease Models, Animal; D010936:Plant Extracts / Q000633:toxicity*; D011817:Rabbits
D010936:Plant Extracts; C539751:ankaferd blood stopper