Comparison of inert supports in laser desorption/ionization mass spectrometry of peptides: pencil lead, porous silica gel, DIOS-chip and NALDI target.
In the search for alternative inert surfaces replacing silicon chips in Desorption/Ionization On porous Silicon (DIOS)-like mass spectrometry analyses, nanostructured silicon-based NALDI chips were evaluated in Laser Desorption/Ionization (LDI) of peptides. Comparisons were made using commercially available DIOS chips (MassPREP-DIOS-target), amorphous carbon powder from lead pencil and porous silica gel used for chromatographic purposes as reference supports. A set of synthetic model peptides presenting variable amino acid sequences of various lengths was analyzed under all conditions. The LDI responses of the four 'matrix-free' techniques were compared, especially in terms of peptide detection sensitivity and overall experiment robustness.
Shenar
Nawar
N
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Universités Montpellier 1 et 2, Bâtiment Chimie (17), Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
Cantel
Sonia
S
Martinez
Jean
J
Enjalbal
Christine
C
D000595:Amino Acid Sequence; D002244:Carbon / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry; D010455:Peptides / Q000032:analysis* / Q000138:chemical synthesis; D016062:Porosity; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties
D005782:Gels; D010455:Peptides; D002244:Carbon; D012822:Silicon Dioxide; D012825:Silicon