[Screening and identification analysis of serum protein biomarkers in nephroblastoma].
OBJECTIVE
To screen and characterize the serum protein biomarkers in nephroblastoma so as to establish the proteins as the specific serum biomarkers for diagnosis and prognosis monitoring.


METHODS
The differential protein peaks were located by detecting serum samples of preoperative and postoperative patients and normal children using the SELDI-TOF MS technology. After purification, the differential proteins were further analyzed by LC-MS/MS and the protein sequences searched in database.


RESULTS
Two peaks with m/z of 6455.5 and 6984.4 were selected as potential biomarkers. They were weakly expressed in nephroblastoma (intensity: 1029 +/- 364, 297 +/- 126) but highly expressed in normal individuals (2108 +/- 837, 753 +/- 226); another peak with m/z of 9190.8 was weakly expressed in preoperative sera (283 +/- 154) but highly expressed in serum samples of postoperative patients and normal children (5974 +/- 657, 6231 +/- 519). The protein at 6455.5 and 9190.8 were identified as apolipoprotein C-III and haptoglobin respectively.


CONCLUSIONS
The detection of differentially expressed apolipoprotein C-III and haptoglobin may have potential utilities for serum diagnosis, malignancy classification and prognosis monitoring of nephroblastoma and is worthy of further studies and applications.
Wang
Jia-Xiang
JX
Department of Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. wjiaxiang@zzu.edu.cn
Yang
Shao-Bo
SB
Liu
Qiu-Liang
QL
Dong
Rui
R
Fan
Yu-Xia
YX
Zhang
Qian
Q
Yang
Fu-Quan
FQ
Wu
Peng
P
Yu
Jie-Kai
JK
Zheng
Shu
S
D053305:Apolipoprotein C-III / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006242:Haptoglobins / Q000032:analysis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009363:Neoplasm Proteins / Q000097:blood; D009367:Neoplasm Staging; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D009396:Wilms Tumor / Q000097:blood* / Q000473:pathology
D053305:Apolipoprotein C-III; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D006242:Haptoglobins; D009363:Neoplasm Proteins