Effect of matrix metalloproteinase-1 promoter genotype on interleukin-1beta-induced matrix metalloproteinase-1 production in human periodontal ligament cells.
OBJECTIVE
Our previous studies have shown that a single nucleotide polymorphism at nucleotide -1607 of the human matrix metalloproteinase-1 (MMP-1) promoter is associated with the severity of periodontal disease. The purpose of this study was to evaluate the effect of different MMP-1 promoter genotypes on interleukin-1beta-induced MMP-1 production in human periodontal ligament cells.


METHODS
Periodontal ligament cells from 16 different subjects were cultured. Restriction fragment length polymorphism-polymerase chain reaction was used to identify the MMP-1 promoter genotype of periodontal ligament cells. Periodontal ligament cells were stimulated with phorbol 12-myristate 13-acetate/interleukin-1beta. Reverse transcription-polymerase chain reaction amplifications and enzyme linked immunosorbent assays were then utilized to determine the MMP-1 mRNA levels and to assess the concentration of MMP-1 protein, respectively.


RESULTS
The results showed that cells with the 1G/2G and the 2G/2G genotype produced higher amounts of MMP-1 protein than cells with the 1G/1G genotype. Induction of MMP-1 mRNA as a result of stimulation with interleukin-1beta was significantly increased in cells with a 1G/2G or a 2G/2G genotype compared with cells homozygous for the 1G allele.


CONCLUSIONS
The results of the present study suggest that the single nucleotide polymorphism at nucleotide -1607 of the human MMP-1 promoter might influence the interleukin-1beta-induced expression of MMP-1 in periodontal ligament cells.
Cao
Z
Z
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Li
C
C
Xiang
J
J
D000293:Adolescent; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D005838:Genotype; D006147:Guanine; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects / Q000235:genetics*; D010513:Periodontal Ligament / Q000166:cytology / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics
D002273:Carcinogens; D053583:Interleukin-1beta; D012333:RNA, Messenger; D006147:Guanine; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D013755:Tetradecanoylphorbol Acetate