Mold fabrication and biological assessment of porous DNA-chitosan complexes.
A previous study revealed that DNA-chitosan complex prepared from the reaction between native DNA and chitosan in aqueous solution has suitable porosity for cell seeding, is nontoxic, and causes only a mild soft-tissue response. This simple and easy fabrication method for porous DNA-chitosan complex provides for a wide variety of applications as a scaffold material. The present study evaluated whether rinsing with PBS solution can fabricate DNA-chitosan complex in a mold and the histopathological responses of rat soft tissues to fabricated DNA-chitosan complexes. DNA-chitosan complex paste was prepared by mixing distilled water and freeze-dried water-rinsed DNA-chitosan complex powder. A DNA-chitosan complex disk could be fabricated by rinsing with PBS buffer and subsequently freeze-drying the DNA-chitosan complex paste in the mold. Thus, a wide range of applications of DNA-chitosan complex for tissue engineering can be anticipated using the present easy fabrication method. The porosity of the disk was 85%, and many pores were visible in the DNA-chitosan complex (before fabrication) and in the fabricated DNA-chitosan disk. The values of the complex disks gradually reduced in the tissues although 60% of disks remained in the tissues. In conclusion, an easy fabrication method for making porous DNA-chitosan complex disks was developed. It was found that the fabrication method can delay the biodegradation of the DNA-chitosan complex disk without serious tissue responses in vivo. DNA-chitosan complex is promising as a scaffold material, and a wide range of applications of DNA-chitosan complex for tissue engineering are anticipated.
Fukushima
Tadao
T
Department of Dental Engineering, Bioengineering Section, Fukuoka Dental College, Sawara-ku, Fukuoka 814-0193, Japan.
Ohno
Jun
J
Department of Morphological Biology, Pathology Section, Fukuoka Dental College, Sawara-ku, Fukuoka 814-0193, Japan.
Hayakawa
Tohru
T
Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
Kawaguchi
Minoru
M
Department of Dental Engineering, Bioengineering Section, Fukuoka Dental College, Sawara-ku, Fukuoka 814-0193, Japan.
Inoue
Yusuke
Y
Fukuoka College of Health Sciences, Sawara-ku, Fukuoka 814-0193, Japan.
Takeda
Shoji
S
Department of Biomaterials, Osaka Dental University, Hirakata, Osaka 573-1121, Japan.
Toyoda
Mika
M
Department of Functional Bioscience, Section of Infection Biology, Fukuoka Dental College, Sawara-ku, Fukuoka 814-0193, Japan.
Okahata
Yoshio
Y
Department of Biomolecular Engineering, Tokyo Institute of Technology, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
D000465:Algorithms; D000818:Animals; D017690:Cell Transplantation; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D020556:Neutrophil Infiltration; D011095:Polyethylenes; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000166:cytology; D014783:Viscosity
D011095:Polyethylenes; D004247:DNA; D048271:Chitosan