title: Analysis of NK cell/DC interaction in NK-type lymphoproliferative disease of granular lymphocytes (LDGL): role of DNAM-1 and NKp30.
abstract: OBJECTIVE
  Natural killer (NK) cells and dendritic cells (DC) can give rise to reciprocal functional interactions resulting in promotion of DC maturation, killing of immature DC (iDC), and proliferation of NK cells. In this study, we analyze whether, in NK-lymphoproliferative disease of granular lymphocytes (LDGL) patients, this function could be altered and contribute to the persistence of the disease.
  
  
  METHODS
  Freshly isolated peripheral blood NK granular lymphocytes (GL) and NK cell lines derived from 13 different NK-LDGL patients were analyzed in coculture experiments to evaluate their ability to interact with monocyte-derived DCs (Mo-DC).
  
  
  RESULTS
  As compared to NK cells isolated from healthy donors, NK-GLs displayed, in most cases, a reduced capability of promoting Mo-DC maturation and of killing iDC. These findings could be explained, at least in part, by the low expression levels of NKp30: an activating receptor involved in the molecular interactions occurring between NK cells and DC. We also show that, in the presence of DC-derived cytokines such as interleukin-12, in both patients and healthy individuals, DNAM-1 can cooperate with NKp30 to induce NK cells to kill DC, release tumor necrosis factor-alpha, and promote DC maturation. This contribution, however, is not sufficient to compensate for the defect in patients' NK cells.
  
  
  CONCLUSIONS
  Besides expanding knowledge of the molecular basis of the NK/DC cross-talk, our study demonstrates that NK cells from NK-LDGL patients are impaired in their ability to interact with Mo-DC. The possible relationship between such abnormal NK cell/DC interactions and chronic NK cell proliferation are discussed.
authors: DI.ME.S. Dipartimento di Medicina Sperimentale, Università di Genova, Genova, Italy.
authors: Mirna
authors: M
authors: Balsamo
authors: Renato
authors: R
authors: Zambello
authors: Antonella
authors: A
authors: Teramo
authors: Marco
authors: M
authors: Pedrazzi
authors: Bianca
authors: B
authors: Sparatore
authors: Francesca
authors: F
authors: Scordamaglia
authors: Daniela
authors: D
authors: Pende
authors: Maria Cristina
authors: MC
authors: Mingari
authors: Lorenzo
authors: L
authors: Moretta
authors: Alessandro
authors: A
authors: Moretta
authors: Gianpietro
authors: G
authors: Semenzato
authors: Massimo
authors: M
authors: Vitale
mesh_terms: D000328:Adult; D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D002450:Cell Communication; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D003713:Dendritic Cells / Q000473:pathology*; D005260:Female; D024243:HMGB1 Protein / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology*; D054066:Leukemia, Large Granular Lymphocytic / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000502:physiology*; D000098862:T Lineage-Specific Activation Antigen 1
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D024243:HMGB1 Protein; D055628:Natural Cytotoxicity Triggering Receptor 3