Oligomycin induced the proteasomal degradation of cyclin D1 protein.
We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.
Kanai
Mai
M
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan.
Iba
Satoru
S
Okada
Ryoko
R
Tashiro
Etsu
E
Imoto
Masaya
M
D000256:Adenoviridae / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D019938:Cyclin D1 / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D019940:Genes, bcl-1 / Q000187:drug effects*; D005822:Genetic Vectors; D006801:Humans; D007202:Indicators and Reagents; D009840:Oligomycins / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles; D014162:Transfection
D007202:Indicators and Reagents; D009840:Oligomycins; D013778:Tetrazolium Salts; D013844:Thiazoles; D019938:Cyclin D1; D046988:Proteasome Endopeptidase Complex; C022616:thiazolyl blue