Proliferation and osteogenic differentiation of rat bone marrow stromal cells on bioapatite with different crystalline facets.
The objective of this study is to investigate the attachment, osteogenic differentiation, and collagen matrix secretion of rat bone marrow stromal cells (MSC) and MC3T3-E1 cells on the biological apatite (Ap) with different crystalline facets. The Ap samples with a-facet and c-facet surfaces were prepared by cutting the heat-treated cortical bone of bovine femurs parallel and perpendicular to the longitudinal direction, respectively. MC3T3-E1 cells attached spreading on the a-Ap sample whereas their shape of cells was round on the c-Ap. No difference in the proliferation profile of MSC and MC3T3-E1 cells was observed between the two samples. The activity of alkaline phosphatase was significantly higher for both the cells on the a-Ap sample than that of c-Ap one. The matrix of collagen secreted from MC3T3-E1 cells on the a-Ap sample oriented along the c-axis of Ap, in contrast to those of c-Ap. The cells tended to align along the collagen orientation. It is concluded that the alignment of cells is influenced by the collagen fibril orientation of Ap samples.
Ichinohe
Norihisa
N
Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University, South 1, West 17, Chuo-ku, Sapporo 060-8556.
Nakano
Takayoshi
T
Mitaka
Toshihiro
T
Umakoshi
Yukichi
Y
Tabata
Yasuhiko
Y
D016475:3T3 Cells; D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*; D013499:Surface Properties; D019096:Vitronectin / Q000378:metabolism; D014961:X-Ray Diffraction
D001031:Apatites; D001672:Biocompatible Materials; D005353:Fibronectins; D019096:Vitronectin; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase