Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics.
The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL).
Lenshof
Andreas
A
Department of Electrical Measurements, Lund University, Box 118, 221 00 Lund, Sweden. andreas.lenshof@elmat.lth.se
Ahmad-Tajudin
Asilah
A
Järås
Kerstin
K
Swärd-Nilsson
Ann-Margret
AM
Aberg
Lena
L
Marko-Varga
György
G
Malm
Johan
J
Lilja
Hans
H
Laurell
Thomas
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D007118:Immunoassay; D008297:Male; D046228:Microarray Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010956:Plasmapheresis / Q000295:instrumentation* / Q000379:methods; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis; D040081:Protein Array Analysis; D012825:Silicon / Q000737:chemistry
D000911:Antibodies, Monoclonal; D017430:Prostate-Specific Antigen; D012825:Silicon