title: Poisoning of sheep by seeds of Crotalaria retusa: acquired resistance by continuous administration of low doses.
abstract: Seeds of Crotalaria retusa containing 6.84% (dry weight) of monocrotaline (MCT) were administered experimentally to sheep. Three sheep that received 136.8mg MCT/kg bw daily for 70 days had no clinical signs. Five out of six sheep ingesting single doses of 205.2 and 273.6mg MCT/kg bw died with acute (three sheep) or chronic intoxication (two sheep). Acute intoxicated sheep had periacinar liver necrosis and chronic intoxicated sheep liver fibrosis and megalocytosis. Another three sheep had no clinical signs after the ingestion of 20 daily doses of 136.8mg MCT/kg, followed by seven doses of 273.6mg MCT/kg, and one single dose of 342mg MCT/kg. These experiments demonstrated that sheep are susceptible to acute intoxication by MCT being intoxicated by a single oral dose of approximately 205.2mg/kg. In contrast, they develop strong resistance to MCT after the daily ingestion of non lethal doses (136.8mg/kg). It is suggested that chronic poisoning does not occur by the repeated ingestion of non acutely toxic doses, but probably by the ingestion of single toxic doses. It is also suggested that sheep do not become intoxicated with the ingestion of C. retusa in the vegetative non-seeding stage.
authors: Veterinary Hospital, CSTR, Federal University of Campina Grande, Patos, 58700-310, Paraíba, Brazil.
authors: Bruno Leite
authors: BL
authors: Anjos
authors: Verônica M T
authors: VM
authors: Nobre
authors: Antônio F M
authors: AF
authors: Dantas
authors: Rosane M T
authors: RM
authors: Medeiros
authors: Temístocles S
authors: TS
authors: Oliveira Neto
authors: Russell J
authors: RJ
authors: Molyneux
authors: Franklin
authors: F
authors: Riet-Correa
mesh_terms: D000821:Animal Feed; D000818:Animals; D001938:Brazil; D029864:Crotalaria / Q000737:chemistry / Q000506:poisoning*; D004361:Drug Tolerance; D007441:Intubation, Gastrointestinal / Q000662:veterinary; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary; D009682:Magnetic Resonance Spectroscopy; D016686:Monocrotaline / Q000008:administration & dosage / Q000032:analysis / Q000506:poisoning*; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary; D010939:Plant Poisoning / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D011763:Pyrrolizidine Alkaloids / Q000032:analysis / Q000506:poisoning; D012639:Seeds / Q000737:chemistry / Q000506:poisoning*; D012757:Sheep Diseases / Q000401:mortality / Q000473:pathology; D034561:Sheep, Domestic
chemical_list: D011763:Pyrrolizidine Alkaloids; D016686:Monocrotaline