title: Potato virus X coat protein fusion to human papillomavirus 16 E7 oncoprotein enhance antigen stability and accumulation in tobacco chloroplast.
abstract: Cervical cancer linked to infection with human papillomavirus (HPV) is the third cause of cancer-related death in women. As the virus cannot be propagated in culture, vaccines have been based on recombinant antigens with inherited high-cost production. In a search of alternative cheap production system, E7 HPV type 16 protein, an attractive candidate for anticancer vaccine development, was engineered to be expressed in tobacco chloroplast. In addition, E7 coding sequence was fused to potato virus X coat protein (CP) to compare expression level. Results show that E7CP transcript accumulation reached lower levels than non-fused E7. However, antigen expression levels were higher for fusion protein indicating that CP stabilizes E7 peptide in the chloroplast stroma. These results support viability of transplastomic plants for antigen production and the relevance of improving recombinant peptide stability for certain transgenes to enhance protein accumulation in this organelle.
authors: Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, C.C. 1428, Ciudad Autónoma de Buenos Aires, Argentina.
authors: Mauro
authors: M
authors: Morgenfeld
authors: María Eugenia
authors: ME
authors: Segretin
authors: Sonia
authors: S
authors: Wirth
authors: Ezequiel
authors: E
authors: Lentz
authors: Alicia
authors: A
authors: Zelada
authors: Alejandro
authors: A
authors: Mentaberry
authors: Lutz
authors: L
authors: Gissmann
authors: Fernando
authors: F
authors: Bravo-Almonacid
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D050725:Papillomavirus E7 Proteins; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D055550:Protein Stability; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011993:Recombinant Fusion Proteins; C058750:coat protein, Potato virus X; C059731:oncogene protein E7, Human papillomavirus type 16