Genotoxicity study of Hypiran and Chamomilla herbal drugs determined by in vivo supervital micronucleus assay with mouse peripheral reticulocytes.
The growth in popularity of Over The Counter (OTC) of medicinal products or other natural sources have taken a very large share of healthcare system therefore it is essential to determine their safety as for as public health is concerned.In this study Maximum Tolerated Dose (MTD) was obtained according to CSGMT protocol presented by the Environmental Mutagen Society of Japan. The positive group received mitomycin C in dose of 0.5 mg/kg. The peripheral blood samples before treatment (zero time) were considered as negative control. The MTD of Hypiron was 12 ml/kg and for Chamomilla was 10 ml/kg. Doses of MTD, 1/2 MTD and 1/4 MTD were considered for test groups. Then blood samples were prepared 48 hours after first administration of drugs and kept on precoated Acridine orange slides. The scoring of micronucleated reticulocytes were carried out per 2000 counted reticulocytes in each slide by fluorescent microscope. The results were statistically analyzed. Results of Hypiran were compared with negative control group and the P value was P > 0.05, but the p value of Chamomilla was P < 0.05. Also, the p value of Hypiran and Chamomilla compared with historical negative control group was less, therefore Chamomilla herbal drog can be classified as equivocul category of genotoxicity and Hypiran did not show genotoxicity.
Kalantari
H
H
School of Pharmacy , Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran. Kalantarih@yahoo.com
Dashtearjandi
A Ashrafi
AA
Kalantar
E
E
D000477:Alkylating Agents / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D020714:Maximum Tolerated Dose; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D016685:Mitomycin / Q000494:pharmacology; D016296:Mutagenesis; D010936:Plant Extracts / Q000378:metabolism*; D028321:Plant Preparations / Q000494:pharmacology*; D012156:Reticulocytes / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D000477:Alkylating Agents; D010936:Plant Extracts; D028321:Plant Preparations; D016685:Mitomycin