Influence of the time between removal and cooling of the canine epididymis on post-thaw caudal epididymal sperm quality.
The effects of the time period between canine epididymis removal and cooling on post-thaw caudal epididymal sperm quality were investigated. Sperm recovered from the epididymis stored for 6 hr at 4 or 20 degrees C exhibited similar motility. However, when the epididymis was stored for 12 hr or longer at 20 degrees C, sperm motility was significantly lower than that at 4 degrees C (p<0.01). The post-thawed qualities of sperm recovered from the caudal epididymides that had been stored at 20 degrees C for 0 or 6 hr and then at 4 degrees C for 24 hr after removal were not significantly different. Therefore, leaving the canine epididymis at 20 degrees C for up to 6 hr after its removal may have little effect on the post-thaw quality of recovered caudal epididymal sperm.
Hori
Tatsuya
T
Department of Reproduction, Nippon Veterinary and Life Science University, 7-1 Kyonan-cho, 1 chome, Musashino-shi, Tokyo 180-8602, Japan. t-hori@nvlu.ac.jp
Uehara
Yuusuke
Y
Kawakami
Eiichi
E
Tsutsui
Toshihiko
T
D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000502:physiology*; D004822:Epididymis / Q000502:physiology*; D008297:Male; D009929:Organ Size / Q000502:physiology; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature; D013997:Time Factors