title: Biofunctionalized tilted Fiber Bragg Gratings for label-free immunosensing.
abstract: We present a study aimed at developing a label-free optical fiber biosensor for detection and quantification of biomolecules in real-time. The biosensor based on a Tilted Fiber Bragg Grating (TFBG) transduces a binding event between the probe and target molecules into a change in the refractive index of the medium surrounding the fiber. This work describes the experimental results obtained with three methods for immobilizing biomolecular probes on a TFBG silica cladding surface. Bovine serum albumin (BSA) and anti-BSA are used to assess the performances of the TFBG based biosensor in each configuration.
authors: CEA, LIST, Laboratoire de Mesures Optiques, Centre d'Etudes de Saclay, Gif-sur-Yvette cedex, France. severine.maguis@cea.fr
authors: Séverine
authors: S
authors: Maguis
authors: Guillaume
authors: G
authors: Laffont
authors: Pierre
authors: P
authors: Ferdinand
authors: Benjamin
authors: B
authors: Carbonnier
authors: Khémara
authors: K
authors: Kham
authors: Tahar
authors: T
authors: Mekhalif
authors: Marie-Claude
authors: MC
authors: Millot
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine