Hypoglycemic activity of dried extracts of Bauhinia forficata Link.
Leaves of the pantropical genus Bauhinia (Fabaceae) are known popularly as cow's foot, due to their unique characteristic bilobed aspect. The species Bauhinia forficata (Brazilian Orchid-tree) is widely used in folk medicine as an antidiabetic. The present work investigates the hypoglycemic activity of the dried extracts of Bauhinia forficata leaves in vivo, as well as the influence of the drying and granulation processes on this activity. The fluid extract was dried to generate oven-dried (ODE), spray-dried (SDE) and wet granulation (WGE) extracts, with the aid of colloidal silicon dioxide and/or cellulose:lactose mixture. The dried extracts were characterized by spectrophotometric, chromatographic and photo microscopy image analysis. 200 mg/kg body wt., p.o. of each dried product were administered orally to male Wistar rats over 7 days old, for biomonitoring of the hypoglycemic activity profile. The effect of the extracts was studied in STZ-induced diabetic rats. After 7 days of treatment, fasting glucose was determined, and the livers were removed, dried on tissue paper, weighed, and stored at -20 degrees C to estimate hepatic glycogen. Our results show that spray-drying or oven-drying processes applied to B. forficata extracts did not significantly alter its flavonoid profile or its hypoglycemic activity. Indeed, the dried extracts of B. forficata act differently from glibenclamide. Despite the lower active content in WGE, because of the higher concentration of adjuvants, the use of the granulation process improved the manufacturing properties of the ODE, making this material more appropriate for use in tablets or capsules.
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Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Curso de Farmácia, CCS, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina (SC), 88302-202, Brazil.
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D000818:Animals; D036481:Bauhinia / Q000737:chemistry*; D001786:Blood Glucose / Q000378:metabolism*; D002626:Chemistry, Pharmaceutical; D003890:Desiccation; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004304:Dosage Forms; D005419:Flavonoids / Q000032:analysis; D005905:Glyburide / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D004304:Dosage Forms; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D005905:Glyburide