[cDNA-AFLP analysis on transcripts associated with bolting in Brassica rapa L. ssp. pekinensis].
Premature bolting, caused by low temperature in spring and summer cultivation in low land and high land respectively, leads to reduction of the yield and quality of the harvested products in Chinese cabbage. Therefore, exploring genes involved in vernalization response is important to the improvement of Chinese cabbage varieties. Here, one extremely early bolting line (DH-54) and one extremely late bolting line (DH-43) were employed, and the cDNA-AFLP approach was used to identify key components involved in the low-temperature required vernalization response. Of 256 primer recombinations screened, a total of 191 differential expressed transcript-derived fragments (TDFs) were identified, and 82 TDFs were sequenced. BLAST and alignments showed that 52 candidate TDFs shared high levels of similarity with genes of known function, 22 TDFs of unknown function and 8 novel ESTs. The TDFs of known function were involved in genes encoding enzymes working in metabolism, proteins related to stress and defense, signal transduction, and transcription regulation, etc.
Zou
Yan-Min
YM
College of Life Science, Capital Normal University, Beijing 100037, China. yanmin5036@sohu.com
Yu
Shuan-Cang
SC
Zhang
Feng-Lan
FL
Yu
Yang-Jun
YJ
Zhao
Xiu-Yun
XY
Zhang
De-Shuang
DS
D054458:Amplified Fragment Length Polymorphism Analysis; D029741:Brassica rapa / Q000235:genetics* / Q000254:growth & development*; D018076:DNA, Complementary / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012621:Seasons; D013696:Temperature
D018076:DNA, Complementary; D012333:RNA, Messenger