A device for automated hydrodynamic shearing of genomic DNA.
We describe a device for automated fragmentation of genomic DNA by hydrodynamic shearing using a filter screen with uniform pores. Human genomic DNA can be fragmented reproducibly to a range of 2000-12,000 bp by using various fluid flow rates and screens with 0.5-10-microm pores. The utilization of disposable screens eliminates sample cross-contamination and the tendency of device clogging, commonly encountered in single-orifice shearing devices.
Joneja
Aric
A
Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
Huang
Xiaohua
X
D001331:Automation / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation*; D016062:Porosity; D015203:Reproducibility of Results
D004247:DNA