[Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants].
The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vector harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.
Yin
Tao
T
Department of Horticulture, Zhejiang University, Hangzhou 310029, China. yintaoo@163.com
Lu
Hong-Yu
HY
Zhang
Shang-Long
SL
Liu
Jing-Mei
JM
Chen
Da-Ming
DM
D001578:Benzopyrans / Q000378:metabolism*; D015139:Blotting, Southern; D029773:Citrullus / Q000235:genetics; D005638:Fruit / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D007192:Indenes / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D012231:Rhizobium / Q000235:genetics; D014162:Transfection
D001578:Benzopyrans; D007192:Indenes; D010940:Plant Proteins; C050295:brazilein