A glycoprotein resembling a peripheral nicotinic acetylcholine receptor that binds [(125)I]-alpha-bungarotoxin in mouse brain.
A protein that binds alpha-bungarotoxin (alpha-BGT) was found in mouse brain and purified by means of Sepharose 6B column chromatography. The binding was inhibited by d-tubocurarine but not by atropine. This component was a glycoprotein with a molecular weight of 7 x 10(5) determined by gel chromatography, an apparent S value of approximately 11 determined by sucrose density gradient centrifugation and an isoelectric point of 5.6. The results showed that the component resembled a nicotinic acetylcholine receptor (AChR) in skeletal muscle and in electric organs.
Seto
A
A
Mitsubishi-Kasei Institute of Life Sciences, 11, Minamiooya, Machida-shi, Tokyo 194 Japan.
Arimatsu
Y
Y
Amano
T
T