Black shank resistant tobacco by silencing of glutathione S-transferase.
A glutathione S-transferase gene was amplified from cDNA of Nicotiana tabacum roots infected with Phytophthora parasitica var. nicotianae. The gene was cloned in sense and anti-sense orientation to an RNAi vector for induced gene silencing, and reduced expression of the gene was detected by RT-PCR. A statistically significant increase in resistance of N. tabacum to infection following gene silencing was found for glutathione S-transferase-silenced plants compared with control plants. Some defense genes were up-regulated in glutathione S-transferase-silenced plants during the interaction with the pathogen. This is the first evidence of the role of glutathione S-transferase as negative regulator of defense response.
Hernández
Ingrid
I
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Havana 10600, Cuba.
Chacón
Osmany
O
Rodriguez
Raisa
R
Portieles
Roxana
R
López
Yunior
Y
Pujol
Merardo
M
Borrás-Hidalgo
Orlando
O
D003001:Cloning, Molecular; D020868:Gene Silencing; D005982:Glutathione Transferase / Q000235:genetics*; D010838:Phytophthora; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D014026:Nicotiana / Q000235:genetics*
D005982:Glutathione Transferase