Rat marrow-derived multipotent adult progenitor cells differentiate into skin epidermal cells in vivo.
Wound repair and functional reconstruction are two key aspects for treatment of skin injury. Research on cell source for skin repair has become a focus of study. The immune rejection induced by allograft cells and the limited source of autologous epidermal stem cells have led to more attention on the multipotent adult progenitor cells (MAPC). In this study, we examined the influence of the local environment of skin injury on the migration and differentiation of MAPC in nude mice. The homing of MAPC to the wounds and the epidermal differentiation of MAPC were investigated by detecting the expression of specific antigens of rat major histocompatibility complex I (MHC-I) antigen and the tracing markers. Three weeks after transplantation, hair follicle-like structure appeared and rat MHC-I antigen was positive in the follicles of the healed skin. PKH26-labeled cells expressing cytokeratin were found in the regenerated follicle-like structures, sebaceous glands and sweat glands. Our findings indicate that MAPC can migrate to the skin injury site and the hair follicles, and participate in skin wound healing by differentiating into epidermal cells, which contributes to the theoretical research of MAPC plasticity and provides theoretical evidence for clinical application of transplantation therapy with MAPC.
Ji
Kai-Hong
KH
Research Center of Developmental Biology, Department of Histology and Embryology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China.
Xiong
Jun
J
Fan
Li-Xing
LX
Hu
Kai-Meng
KM
Liu
Hou-Qi
HQ
D053687:Adult Stem Cells / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D000831:Animals, Newborn; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002465:Cell Movement; D000078404:Epidermal Cells; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D012867:Skin / Q000276:immunology / Q000293:injuries / Q000473:pathology; D014183:Transplantation, Heterologous; D014945:Wound Healing
D015395:Histocompatibility Antigens Class I