title: Association study between BDNF gene polymorphisms and autism by three-dimensional gel-based microarray.
abstract: Single nucleotide polymorphisms (SNPs) are important markers which can be used in association studies searching for susceptible genes of complex diseases. High-throughput methods are needed for SNP genotyping in a large number of samples. In this study, we applied polyacrylamide gel-based microarray combined with dual-color hybridization for association study of four BDNF polymorphisms with autism. All the SNPs in both patients and controls could be analyzed quickly and correctly. Among four SNPs, only C270T polymorphism showed significant differences in the frequency of the allele (chi(2) = 7.809, p = 0.005) and genotype (chi(2) = 7.800, p = 0.020). In the haplotype association analysis, there was significant difference in global haplotype distribution between the groups (chi(2) = 28.19, p = 3.44e-005). We suggest that BDNF has a possible role in the pathogenesis of autism. The study also show that the polyacrylamide gel-based microarray combined with dual-color hybridization is a rapid, simple and high-throughput method for SNPs genotyping, and can be used for association study of susceptible gene with disorders in large samples.
authors: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
authors: Lu
authors: L
authors: Cheng
authors: Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China.
authors: Qinyu
authors: Q
authors: Ge
authors: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
authors: Pengfeng
authors: P
authors: Xiao
authors: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
authors: Beili
authors: B
authors: Sun
authors: Child Mental Health Research Center of Nanjing Brain Hospital affiliated of Nanjing Medical University, Nanjing, 210029, China.
authors: Xiaoyan
authors: X
authors: Ke
authors: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.
authors: Yunfei
authors: Y
authors: Bai
authors: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.|Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China.
authors: Zuhong
authors: Z
authors: Lu
mesh_terms: D000483:Alleles; D001321:Autistic Disorder / Q000235:genetics*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3