A stable fish reporter cell line to study estrogen receptor transactivation by environmental (xeno)estrogens.
Cross-species differences between human and fish estrogen receptor (ER) binding by environmental chemicals have been reported. To study ER transactivation in a fish cellular context, we stably co-transfected the PLHC-1 fish hepatoma cell line with a rainbow trout estrogen receptor (rtER) and the luciferase reporter gene driven by an estrogen response element (ERE). This new cell model, called PELN-rtER (for PLHC-1-ERE-Luciferase-Neomycin), responded to 17beta-estradiol (E2) in a both concentration- and temperature-dependent manner, as well as to environmental ER ligands from different chemical classes: natural and synthetic estrogens, zearalenone metabolites, genistein, alkyphenoles and benzophenone derivatives. The comparison with other in vitro models, i.e. human reporter cell lines (HELN-rtER, MELN) and vitellogenin induction in primary cultures of rainbow trout hepatocytes, showed an overall higher sensitivity of the human cells for a majority of ligands, except for benzophenone derivatives which were active at similar or lower concentrations in fish cells, suggesting species-specificity for these substances. Correlation analyses suggest that the fish cell line is closer to the trout hepatocyte than to the human cell context, and could serve as a relevant mechanistic tool to study ER activation in fish hepatic cellular context.
Cosnefroy
A
A
INERIS, Unité d'Ecotoxicologie, BP2, f-60550 Verneuil-en-Halatte, France.
Brion
F
F
Guillet
B
B
Laville
N
N
Porcher
J M
JM
Balaguer
P
P
Aït-Aïssa
S
S
D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005399:Fishes; D019833:Genistein / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects; D008156:Luciferases / Q000235:genetics; D008297:Male; D017686:Oncorhynchus mykiss; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000235:genetics; D013045:Species Specificity; D013696:Temperature; D015533:Transcriptional Activation / Q000187:drug effects*; D014819:Vitellogenins / Q000096:biosynthesis; D015029:Zeranol / Q000494:pharmacology
D004967:Estrogens; D011960:Receptors, Estrogen; D014819:Vitellogenins; D004958:Estradiol; D015029:Zeranol; D019833:Genistein; D008156:Luciferases