Standardized preparation of single-cell suspensions from mouse lung tissue using the gentleMACS Dissociator.
The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator optimizes this task with a simple, practical protocol. The instrument contains pre-programmed settings that are optimized for the efficient but gentle dissociation of a variety of tissue types, including mouse lungs. In this publication the use of the gentleMACS Dissociator on lung tissue derived from mice is demonstrated.
Jungblut
Melanie
M
Miltenyi Biotec,GmbH.
Oeltze
Karen
K
Zehnter
Irene
I
Hasselmann
Doris
D
Bosio
Andreas
A
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003713:Dendritic Cells / Q000166:cytology; D005260:Female; D008168:Lung / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013535:Suspensions
D013535:Suspensions