Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse.
We demonstrate two complementary types of microscopy using an identical setup for single-pulse coherent anti-Stokes Raman scattering (CARS) imaging, which employs an ultrabroadband laser pulse with a spectral bandwidth of 4800 cm(-1) and enables the suppression of nonresonant CARS signals. One is a novel type of microscopy that uses spectral phase modulation for the selective excitation of a single Raman mode. The selective excitation is achieved by the modulated pulse focusing its difference-frequency spectrum into a narrow spectral region. Another type is Fourier-transform CARS (FT-CARS) microspectroscopy based on the measurement of the CARS spectrum obtained from the Fourier-transform of the interferometric autocorrelation (IAC) signal. Vibrational spectral imaging of chemical and biological samples is demonstrated using the two types of microscopy.
Isobe
Keisuke
K
Keisuke Isobe Laser Technology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kisobe@riken.jp
Suda
Akira
A
Tanaka
Masahiro
M
Hashimoto
Hiroshi
H
Kannari
Fumihiko
F
Kawano
Hiroyuki
H
Mizuno
Hideaki
H
Miyawaki
Atsushi
A
Midorikawa
Katsumi
K
D000465:Algorithms; D004867:Equipment Design; D005583:Fourier Analysis; D006367:HeLa Cells; D006801:Humans; D007368:Interferometry / Q000379:methods; D007834:Lasers; D008928:Mitochondria / Q000473:pathology; D055095:Optics and Photonics; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors