Successful spinal fusion by E. coli-derived BMP-2-adsorbed porous beta-TCP granules: a pilot study.
Bone morphogenetic proteins (BMPs) were originally identified as osteoinductive proteins. With cloning of BMP genes, studies of BMPs and their clinical application have advanced. However, with increasing clinical applications, drug delivery systems and production costs have become more important issues. To address these issues, we asked whether E. coli-derived rhBMP-2 (E-BMP-2)-adsorbed porous beta-TCP granules could achieve posterolateral lumbar fusion in a rabbit model similar to autogenous bone grafts. Lumbar spinal fusion masses were evaluated by 3-D computed tomography, mechanical testing, and histological analyses 8 weeks after surgery. By these measures E-BMP-2-adsorbed beta-TCP granules achieved lumbar spinal fusion in dose-dependent fashion in a rabbit model as well as autogenous bone graft. Our preliminary findings suggest E-BMP-2-adsorbed porous beta-TCP could be a novel, effective alternative to autogenous bone grafting for generating new bone and promoting regenerative repair of bone, and potentially utilizable in the clinical setting for treating spinal disorders.
Dohzono
Sho
S
Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, Osaka 545-8585, Japan.
Imai
Yuuki
Y
Nakamura
Hiroaki
H
Wakitani
Shigeyuki
S
Takaoka
Kunio
K
D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis* / Q000235:genetics; D001861:Bone Regeneration / Q000187:drug effects; D016723:Bone Remodeling / Q000187:drug effects; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000503:physiopathology / Q000601:surgery*; D010865:Pilot Projects; D016062:Porosity; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D013123:Spinal Fusion / Q000379:methods*; D013997:Time Factors; D014057:Tomography, X-Ray Computed; D014182:Transplantation, Autologous
C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D004337:Drug Carriers; D011994:Recombinant Proteins; C485817:beta-tricalcium phosphate