Comparison of the response of cultured osteoblasts and osteoblasts outgrown from rat calvarial bone chips to nonfouling KRSR and FHRRIKA-peptide modified rough titanium surfaces.
Mimicking proteins found in the extracellular matrix (ECM) using specific peptide sequences is a well-known strategy for the design of biomimetic surfaces, but has not yet been widely exploited in the field of biomedical implants. This study investigated osteoblast and, as a control, fibroblast proliferation to novel consensus heparin-binding peptides sequences KRSR and FHRIKKA that were immobilized onto rough (particle-blasted and chemically etched) commercially pure titanium surfaces using a poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) molecular assembly system. This platform enabled a detailed study of specific cell-peptide interactions even in the presence of serum in the culture medium; thanks to the excellent nonfouling properties of the PLL-g-PEG surface. Cell-binding peptide sequence RGD in combination with KRSR or FHRRIKA was used to examine a potentially-enhanced or synergistic effect on osteoblast proliferation. Bare titanium and bioinactive surfaces (i.e., unfunctionalized PLL-g-PEG and scrambled KSSR, RFHARIK, and RDG) were used as control substrates. Additionally, in a newly developed experimental setup, freshly harvested bone chips from newborn rat calvariae were placed onto the same type of surfaces investigating size and pattern of osteoblast outgrowths. The findings of the current study demonstrated that the difference in osteoblast and fibroblast proliferation was influenced by surface topography more so than by the presence of surface-bound KRSR and FHRRIKA. On the other hand, in comparison with the control surfaces, osteoblast outgrowths from rat calvarial bone chips covered a significantly larger area on RGD, KRSR, and FHRRIKA surfaces after 8 days and also migrated in an isotropic way unlike cells on the bioinactive substrates. Furthermore, the stimulatory effect of 0.75 pmol cm(-2) RGD on osteoblast migration pattern could be enhanced when applied in combination with 2.25 pmol cm(-2) KRSR.
Schuler
Martin
M
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, 8093 Zurich, Switzerland.|Institut Straumann AG, 4052 Basel, Switzerland.
Hamilton
Douglas W
DW
Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
Kunzler
Tobias P
TP
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, 8093 Zurich, Switzerland.
Sprecher
Christoph M
CM
AO Research Institute, 7270 Davos, Switzerland.
de Wild
Michael
M
Institut Straumann AG, 4052 Basel, Switzerland.
Brunette
Donald M
DM
Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
Textor
Marcus
M
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, 8093 Zurich, Switzerland.
Tosatti
Samuele G P
SGP
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, 8093 Zurich, Switzerland.
D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000166:cytology; D002452:Cell Count; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects; D066298:In Vitro Techniques; D008422:Materials Testing; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects*; D011092:Polyethylene Glycols; D011107:Polylysine / Q000031:analogs & derivatives; D019736:Prostheses and Implants; D051381:Rats; D012886:Skull / Q000166:cytology*; D013499:Surface Properties; D014025:Titanium
D009842:Oligopeptides; C437191:lysyl-arginyl-seryl-arginine; C117924:mPEG-block-PLL; C546710:phenylalanyl-histidyl-arginyl-arginyl-isoleucyl-lysyl-alanine; D011107:Polylysine; D011092:Polyethylene Glycols; D014025:Titanium