title: TRPV2 expression in rat oral mucosa.
abstract: The oral mucosa is a highly specialised, stratified epithelium that confers protection from infection and physical, chemical and thermal stimuli. The non-keratinised junctional epithelium surrounds each tooth like a collar and is easily attacked by foreign substances from the oral sulcus. We found that TRPV2, a temperature-gated channel, is highly expressed in junctional epithelial cells, but not in oral sulcular epithelial cells or oral epithelial cells. Dual or triple immunolabelling with immunocompetent cell markers also revealed TRPV2 expression in Langerhans cells and in dendritic cells and macrophages. Electron microscopy disclosed TRPV2 immunoreactivity in the unmyelinated and thinly myelinated axons within the connective tissue underlying the epithelium. TRPV2 labelling was also observed in venule endothelial cells. The electron-dense immunoreaction in junctional epithelial cells, macrophages and neural axons occurred on the plasma membrane, on invaginations of the plasma membrane and in vesicular structures. Because TRPV2 has been shown to respond to temperature, hypotonicity and mechanical stimuli, gingival cells expressing TRPV2 may act as sensor cells, detecting changes in the physical and chemical environment, and may play a role in subsequent defence mechanisms.
authors: Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors: Daiji
authors: D
authors: Shimohira
authors: Mizuho A
authors: MA
authors: Kido
authors: Atsushi
authors: A
authors: Danjo
authors: Tomoka
authors: T
authors: Takao
authors: Bing
authors: B
authors: Wang
authors: Jing-Qi
authors: JQ
authors: Zhang
authors: Takayoshi
authors: T
authors: Yamaza
authors: Sadahiko
authors: S
authors: Masuko
authors: Masaaki
authors: M
authors: Goto
authors: Teruo
authors: T
authors: Tanaka
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism* / Q000648:ultrastructure; D003713:Dendritic Cells / Q000378:metabolism; D004846:Epithelial Attachment / Q000378:metabolism* / Q000648:ultrastructure; D005881:Gingiva / Q000378:metabolism / Q000648:ultrastructure; D007801:Langerhans Cells / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D009061:Mouth Mucosa / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D050916:TRPV Cation Channels / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D050916:TRPV Cation Channels; C476722:Trpv2 protein, rat