title: Selective protein adsorption on polymer patterns formed by self-organization and soft lithography.
abstract: Thin films, with both isotropic and ordered patterns of polymer domains, are used as substrates to study selective adsorption of two proteins (concanavalin A and lentil lectin) and to test reconstruction of polymer patterns by these proteins. Integral geometry approach is used to compare quantitatively fluorescence micrographs of protein patches with AFM images of original isotropic patterns, formed during blend casting of polystyrene/poly(methyl methacrylate) and PS/poly(ethylene oxide). Preferential adsorption of both lectins to PMMA phase domains, enhanced for PS/PMMA interfaces is concluded. In turn, protein binding to PS phase regions of PS/PEO blends is highly selective. Ordered protein grouping is obtained as a result of selective adsorption to alternating stripes of polystyrene (partly brominated to enable identification) and cross-linked PEO, prepared with solvent-assisted micromolding applied to PBrS/PEO bilayers. Biological activity test, performed with concanavalin A, confirms preserved functionality of a complementary protein, carboxypeptidase Y, adsorbed to polymer patterns.
authors: Smoluchowski Institute of Physics, Jagiellonian University, 30-059 Krakow, Poland.
authors: Joanna
authors: J
authors: Zemła
authors: Małgorzata
authors: M
authors: Lekka
authors: Joanna
authors: J
authors: Raczkowska
authors: Andrzej
authors: A
authors: Bernasik
authors: Jakub
authors: J
authors: Rysz
authors: Andrzej
authors: A
authors: Budkowski
mesh_terms: D000327:Adsorption; D043402:Cathepsin A / Q000378:metabolism*; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D037121:Plant Lectins; D011108:Polymers; C033619:lentil lectin; D003208:Concanavalin A; D019904:Polymethyl Methacrylate; D043402:Cathepsin A