Three-dimensional holographic imaging of living tissue using a highly sensitive photorefractive polymer device.
Photorefractive materials are dynamic holographic storage media that are highly sensitive to coherent light fields and relatively insensitive to a uniform light background. This can be exploited to effectively separate ballistic light from multiply-scattered light when imaging through turbid media. We developed a highly sensitive photorefractive polymer composite and incorporated it into a holographic optical coherence imaging system. This approach combines the advantages of coherence-domain imaging with the benefits of holography to form a high-speed wide-field imaging technique. By using coherence-gated holography, image-bearing ballistic light can be captured in real-time without computed tomography. We analyzed the implications of Fourier-domain and image-domain holography on the field of view and image resolution for a transmission recording geometry, and demonstrate holographic depth-resolved imaging of tumor spheroids with 12 microm axial and 10 microm lateral resolution, achieving a data acquisition speed of 8 x 10(5) voxels/s.
Salvador
M
M
Department Chemie, University of Cologne, Luxemburgerstr 116, 50939 Cologne, Germany.
Prauzner
J
J
Köber
S
S
Meerholz
K
K
Turek
J J
JJ
Jeong
K
K
Nolte
D D
DD
D000818:Animals; D001706:Biopsy / Q000295:instrumentation / Q000379:methods; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005583:Fourier Analysis; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D008027:Light; D055095:Optics and Photonics; D012516:Osteosarcoma / Q000473:pathology; D011108:Polymers / Q000737:chemistry; D051381:Rats; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods
D011108:Polymers