Healing process of incisor teeth of diabetic rats replanted after storage in milk.
Several local factors that influence the healing process of replanted teeth have been investigated. However, it remains unclear how systemic alterations, such as diabetes mellitus, affect the prognosis of these cases. The purpose of this study was to evaluate the healing process of incisors of non-controlled diabetic rats replanted after storage in bovine long shelf-life (UHT) whole milk. Thirty-two rats were randomly assigned to receive an endovenous injection of either citrate buffer solution (group I - control; n = 16) or streptozotocin dissolved in citrate buffer solution to induce diabetes (group II; n = 16). After confirmation of the diabetic status by analysis of the glycemic levels, the maxillary right incisor of each animal was extracted and immersed in milk for 60 min. The root canals of teeth were then instrumented, and were filled with a calcium hydroxide-based dressing and replanted into their sockets. All animals received systemic antibiotic and were killed by anesthetic overdose 10 and 60 days after replantation. The specimens containing the replanted teeth were removed, fixed, decalcified, and embedded in paraffin. Semi-serial 6-microm-thick sections were obtained and stained with hematoxylin and eosin for histologic and histometric analyses. The results showed that the connective tissue adjacent to the root surface was less organized in the diabetic animals than in the control animals in both periods; the root dentin was less severely affected by root resorption in the diabetic rats; there were no significant differences between the control and diabetic groups regarding the occurrence of replacement resorption and inflammatory resorption.
Ricieri
Camila Benez
CB
Oral and Maxillofacial Surgery and Traumatology, Dental School of Araçatuba, São Paulo State University, Araçatuba, São Paulo, SP, Brazil.
Sonoda
Celso Koogi
CK
Aranega
Alessandra Marcondes
AM
Panzarini
Sônia Regina
SR
Poi
Wilson Roberto
WR
Sundefeld
Maria Lúcia Marçal Mazza
ML
Okamoto
Tetuo
T
D000818:Animals; D017742:Dental Care for Chronically Ill; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology / Q000601:surgery*; D004195:Disease Models, Animal; D007180:Incisor; D008137:Longitudinal Studies; D008297:Male; D008892:Milk; D019188:Organ Preservation Solutions; D010519:Periodontium / Q000473:pathology* / Q000601:surgery; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012390:Root Canal Therapy; D012391:Root Resorption / Q000473:pathology*; D018709:Statistics, Nonparametric; D014021:Tissue Preservation / Q000379:methods; D014090:Tooth Replantation / Q000379:methods*; D014092:Tooth Root / Q000473:pathology / Q000601:surgery; D019553:Tooth, Nonvital; D014945:Wound Healing
D019188:Organ Preservation Solutions