Solid-phase extraction and analysis of paroxetine in human plasma by ultra performance liquid chromatography-electrospray ionization mass spectrometry.
A rapid, sensitive and rugged solid-phase extraction ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for determination of paroxetine in human plasma. The procedure for sample preparation includes simple SPE extraction procedure coupled with Hypersil Gold C(18) column (100 mm - 2.1 mm, i.d., 1.9 microm) with isocratic elution at a flow-rate of 0.350 mL/min and fluoxetine was used as the internal standard. The analysis was performed on a triple-quadrupole tandem mass spectrometer by multiple reactions monitoring mode via electrospray ionization. Using 500 microL plasma, the methods were validated over the concentration range 0.050-16.710 ng/mL for paroxetine, with a lower limit of quantification of 0.050 ng/mL. The intra- and inter-day precision and accuracy of the quality control samples were within 10.0%. The recovery was 69.2 and 74.4% for paroxetine and fluoxetine respectively. Total run time was only 1.9 min. The method was highly reproducible and gave peaks with excellent chromatography properties.
Bhatt
Mitesh
M
Department of Chemistry, Shree U.P.Arts, Smt.M.G.Panchal Science & V.L.Shah Commerce College Pilvai, Vijapur, Gujarat State, India. mitu_070980@yahoo.co.in
Shah
Sanjay
S
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005473:Fluoxetine / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D017374:Paroxetine / Q000097:blood*; D010949:Plasma / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization
D005473:Fluoxetine; D007202:Indicators and Reagents; D017374:Paroxetine; D017367:Selective Serotonin Reuptake Inhibitors