title: Trypanosoma cruzi: Different methods of data analysis to evaluate the genetics-biology relationship.
abstract: The correlation of genetic and biological diversity in Trypanosoma cruzi was studied. Strains of T. cruzi II, isolated from humans; and of T. cruzi I, isolated from wild-animal reservoirs and from triatomines in the state of Paraná, Brazil, were used. Thirty-six biological parameters measured in vitro and six in vivo, related to growth kinetics and metacyclogenesis, susceptibility to benznidazole, macrophage infection, and experimental infection in mice were evaluated. Data from RAPD and SSR-PCR were used as genetic parameters. Mantel's test, group analysis, principal components analysis (PCA), and cladistical analyses were applied. With the Mantel's test, a low correlation was observed when parameters related to growth kinetics and metacyclogenesis in vitro and development of the experimental infection in vivo were included. The group analysis defined two groups that were separated as to whether they produced patent parasitemia in BALB/c mice. In the larger group, strains derived from wild reservoirs were separated from strains derived from triatomines and humans. The PCA identified two groups that differed as to whether they produced a parasitemia curve in mice. The cladistical analysis supported the previous results. This study shows the importance of the parasite-host relationship for the behavior of the strains, and that the combination of methods supports, extends, and clarifies the available information.
authors: Programa de Pós-graduação em Ciências da Saúde/UEM, Brazil.
authors: Eliane R P
authors: ER
authors: Lala
authors: Miriam H
authors: MH
authors: Andó
authors: Leila
authors: L
authors: Zalloum
authors: Marta
authors: M
authors: Bértoli
authors: Márcia
authors: M
authors: de Oliveira Machado Dalalio
authors: Thais Gomes Verzignassi
authors: TG
authors: Silveira
authors: Mônica L
authors: ML
authors: Gomes
authors: Terezinha A
authors: TA
authors: Guedes
authors: Silvana Marques
authors: SM
authors: de Araújo
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D048293:Didelphis; D004197:Disease Reservoirs; D005819:Genetic Markers; D006801:Humans; D007303:Insect Vectors; D008018:Life Cycle Stages / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D015999:Multivariate Analysis; D010203:Panstrongylus; D016133:Polymerase Chain Reaction / Q000379:methods; D025341:Principal Component Analysis; D019105:Random Amplified Polymorphic DNA Technique; D013223:Statistics as Topic / Q000379:methods*; D014224:Triatoma; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics* / Q000254:growth & development*
chemical_list: D005819:Genetic Markers