Neuroprotective effect of defatted sesame seeds extract against in vitro and in vivo ischemic neuronal damage.
Sesame (Sesamum indicum L.) is an important oilseed crop that possesses a wide spectrum of pharmacological activities. Many studies have been conducted to investigate its health-promoting effects. Compared to other plant oils, sesame seed oil is highly stable to oxidation and has been demonstrated to have protective effects against ischemia-reperfusion injury in the rat brain. However; the effects of defatted sesame seeds extract (DSE) have not been studied yet. The purpose of this study was to evaluate the protective effect of DSE against ischemia models. For in vitro ischemia, oxygen-glucose deprivation followed by reoxygenation (OGD-R, 4 h OGD followed by 24 h reoxygenation) in HT22 cells was used to investigate the protective effects on cell death and the inhibitory effects on lipid peroxidation. For in vivo ischemia, the middle cerebral artery occlusion (MCAo, 2 h of MCAo followed by 22 h of reperfusion) rat model was used. Twenty-two h after occlusion the rats were assessed for neurobehavioral deficit and infarct volume. DSE (0.1-10 microg/mL) significantly reduced the cell death and inhibited lipid peroxidation induced by OGD-R. DSE (30, 100 and 300 mg/kg, p.o.) given twice at 0 h and 2 h after onset of ischemia reduced brain infarct volume dose-dependently and improved sensory-motor function. The therapeutic time window of DSE (300 mg/kg, p.o.) was 2 h after MCAo in rats. In conclusion, our results show that DSE may be effective in ischemia models by an antioxidative mechanism.
Jamarkattel-Pandit
Nirmala
N
Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul, Korea.
Pandit
Naba Raj
NR
Kim
Mi-Yeon
MY
Park
Si Hyung
SH
Kim
Kwan Su
KS
Choi
Hoyoung
H
Kim
Hocheol
H
Bu
Youngmin
Y
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects; D020520:Brain Infarction / Q000188:drug therapy*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002536:Cerebral Arteries; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005947:Glucose; D007511:Ischemia / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D010100:Oxygen; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D012639:Seeds; D031685:Sesamum
D018696:Neuroprotective Agents; D010936:Plant Extracts; D005947:Glucose; D010100:Oxygen