Bronchial nitric oxide is related to symptom relief during fluticasone treatment in COPD.
High levels of exhaled nitric oxide (NO) predict favourable response to inhaled corticosteroids in asthma, but the ability of exhaled NO or inflammatory markers in exhaled breath condensate (EBC) to predict steroid responsiveness in chronic obstructive pulmonary disease (COPD) is not known. We measured alveolar and bronchial NO output, levels of leukotriene B(4) (LTB(4)), cysteinyl leukotrienes (cysLTs) and 8-isoprostane in EBC, spirometry, body plethysmography and symptoms in 40 subjects with COPD before and after 4 weeks of treatment with inhaled fluticasone (500 microg b.i.d.). Five subjects (12.5%) with COPD had significant improvement in lung function during fluticasone treatment, whereas 20 subjects (50%) had significant decrease in symptoms. High baseline bronchial NO flux was associated with higher increase in forced expiratory volume in 1 s to forced vital capacity ratio (r = 0.334, p = 0.038) and more symptom relief (r = -0.317, p = 0.049) during the treatment. Baseline EBC levels of LTB(4), cysLTs or 8-isoprostane were not related to response to fluticasone treatment. Inhaled fluticasone decreased bronchial NO flux but not alveolar NO concentration or markers in EBC. High levels of bronchial NO flux are related to symptom relief and improvement of airway obstruction during treatment with inhaled fluticasone in COPD. Markers of inflammation or oxidative stress in EBC are not related to steroid responsiveness in COPD.
Lehtimäki
L
L
The Immunopharmacology Research Group, Medical School, University of Tampere, Tampere, Finland. lauri.lehtimaki@uta.fi
Kankaanranta
H
H
Saarelainen
S
S
Annila
I
I
Aine
T
T
Nieminen
R
R
Moilanen
E
E
D000730:Androstadienes / Q000627:therapeutic use*; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests; D001980:Bronchi / Q000378:metabolism*; D001993:Bronchodilator Agents / Q000627:therapeutic use*; D005260:Female; D000068298:Fluticasone; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy*
D000730:Androstadienes; D015415:Biomarkers; D001993:Bronchodilator Agents; D009569:Nitric Oxide; D000068298:Fluticasone