title: Standardized preparation of single-cell suspensions from mouse lung tissue using the gentleMACS Dissociator.
abstract: The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator optimizes this task with a simple, practical protocol. The instrument contains pre-programmed settings that are optimized for the efficient but gentle dissociation of a variety of tissue types, including mouse lungs. In this publication the use of the gentleMACS Dissociator on lung tissue derived from mice is demonstrated.
authors: Miltenyi Biotec,GmbH.
authors: Melanie
authors: M
authors: Jungblut
authors: Karen
authors: K
authors: Oeltze
authors: Irene
authors: I
authors: Zehnter
authors: Doris
authors: D
authors: Hasselmann
authors: Andreas
authors: A
authors: Bosio
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003713:Dendritic Cells / Q000166:cytology; D005260:Female; D008168:Lung / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013535:Suspensions
chemical_list: D013535:Suspensions