Transcription independent insulation at TFIIIC-dependent insulators.
Chromatin insulators separate active from repressed chromatin domains. In yeast the RNA pol III transcription machinery bound to tRNA genes function with histone acetylases and chromatin remodelers to restrict the spread of heterochromatin. Our results collectively demonstrate that binding of TFIIIC is necessary for insulation but binding of TFIIIB along with TFIIIC likely improves the probability of complex formation at an insulator. Insulation by this transcription factor occurs in the absence of RNA polymerase III or polymerase II but requires specific histone acetylases and chromatin remodelers. This analysis identifies a minimal set of factors required for insulation.
Valenzuela
Lourdes
L
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California 95064, USA.
Dhillon
Namrita
N
Kamakaka
Rohinton T
RT
D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D002874:Chromosome Mapping; D051548:Histone Acetyltransferases / Q000378:metabolism; D038101:Insulator Elements / Q000502:physiology*; D030781:Organisms, Genetically Modified; D055503:Protein Multimerization; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology; D019474:Transcription Factors, TFIII / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics* / Q000502:physiology; D015003:Yeasts / Q000235:genetics; D000098832:Transcription Factor TFIIIC
D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D012343:RNA, Transfer; D051548:Histone Acetyltransferases