Functionalizable and ultra stable nanoparticles coated with zwitterionic poly(carboxybetaine) in undiluted blood serum.
A new surface chemistry presenting an abundance of functional groups for ligand immobilization in an ultra-low fouling background all in one material for nanoparticles was introduced. This surface platform, as demonstrated by zwitterionic poly(carboxybetaine acrylamide) (polyCBAA) coated nanoparticles, is not only ultra stable in undiluted human blood serum, but also can be conjugated to biomolecules conveniently and effectively. Thus, this surface chemistry is ideal to create multi-functional nanoparticles for targeted delivery and diagnostics. In addition, this work clearly shows that 10% blood serum commonly used to evaluate the stability of nanoparticles is insufficient and a new evaluation criterion with undiluted blood serum is recommended.
Yang
Wei
W
Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA 98195, USA.
Zhang
Lei
L
Wang
Shanlin
S
White
Andrew D
AD
Jiang
Shaoyi
S
D000178:Acrylamides / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*
D000178:Acrylamides; D020099:Coated Materials, Biocompatible; D011108:Polymers; C533866:poly(carboxybetaine acrylamide)