[Cytocompatibility study of Arg-Gly-Asp-recombinant spider silk protein/poly vinyl alcohol scaffold].
OBJECTIVE
To evaluate the cytocompatibility of Arg-Gly-Asp-recombinant spider silk protein (pNSR16)/ poly vinyl alcohol (PVA) through in vitro cytotoxicity experiment and cell-material co-culture experiment.


METHODS
pNSR16/PVA scaffold and its extraction were prepared by using solvent casting/particulate leaching method, and NIH-3T3 cells were cultivated with the extraction in vitro. The cytotoxicity of scaffold was analyzed using MTT assay 1, 3 and 5 days after culture. Scanning electron microscope and HE staining observation were conducted 2, 4 and 6 days after culturing NIH-3T3 cells on the pNSR16/PVA scaffold. Immunohistochemistry detection was performed 6 days after co-culture. Adhesion, growth and expression of the cells on the scaffold were observed.


RESULTS
The cytotoxicity of pNSR16/PVA scaffold was in grade 0. Scanning electron microscope observation: the cells covered the surface of the scaffold and were arranged in a directional manner 4 days after co-culture. HE staining: the cells adhered to and grew on the surface of scaffold, and migrated into the scaffold with the increase of culture duration. Immunohistochemistry detection: bFGF was secreted by NIH-3T3 cells, and the cells differentiated normally.


CONCLUSIONS
pNSR16/PVA scaffold has a satisfactory cytocompatibility and may be an ideal tissue engineered scaffold material.
Wang
Hongxin
H
College of Life Sciences of Fujian Normal University, Fuzhou Fujian, 350108, PR China.
Wei
Meihong
M
Xue
Zhengxiang
Z
Li
Min
M
D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D003601:Cytotoxicity Tests, Immunologic; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D009842:Oligopeptides; D047011:Silk; C047981:arginyl-glycyl-aspartic acid; D011142:Polyvinyl Alcohol