Pronase-free B-cell flow-cytometry crossmatch.
Detection of anti-class II antibodies by panel response assay (PRA) and flow cross-match techniques carries an important value in terms of graft function. Even low levels of pre-formed alloantibodies to HLA class II antigens represent a risk of rejection. We present here a method for blocking non-specific flow crossmatch reactions using pooled, heat-inactivated rabbit serum. This method shows very low background and minimal non-specific reactions. In addition, it avoids the use pronase enzyme that can non-specifically digest different cell surface proteins.
Hajeer
A H
AH
College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia. hajeera@ngha.med.sa
Saleh
S
S
Sutton
P
P
Shubaili
A
A
Anazi
H
H
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006649:Histocompatibility Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods; D011817:Rabbits; D012680:Sensitivity and Specificity
D006649:Histocompatibility Antigens