Thermophilic biohydrogen production by an anaerobic heat treated-hot spring culture.
Batch experiments were conducted to investigate the thermophilic biohydrogen production using an enrichment culture from a Turkish hot spring. Following the enrichment, the culture was heat treated at 100 degrees C for 10 min to select for spore-forming bacteria. H(2) production was accompanied by production of acetate, butyrate, lactate and ethanol. H(2) production was associated by acetate-butyrate type fermentation while accumulation of lactate and ethanol negatively affected the H(2) yield. H(2) production was highest in the temperature range from 49.6 to 54.8 degrees C and optimum values for initial pH and concentrations of iron, yeast extract and glucose were 6.5, 40 mg/l, 4-13.5 g/l, respectively. PCR-DGGE profiling showed that the heat treated culture consisted of species closely affiliated to genus Thermoanaerobacterium.
Karadag
Dogan
D
Tampere University of Technology, Department of Chemistry and Bioengineering, Tampere, Finland. dkaradag@yildiz.edu.tr
Mäkinen
Annukka E
AE
Efimova
Elena
E
Puhakka
Jaakko A
JA
D000085:Acetates / Q000737:chemistry; D000693:Anaerobiosis; D002087:Butyrates / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D016133:Polymerase Chain Reaction; D046950:Thermoanaerobacterium / Q000378:metabolism; D014871:Water Microbiology
D000085:Acetates; D002087:Butyrates; D006859:Hydrogen; D007501:Iron; D005947:Glucose