Stereospecific degradation of phenylsuccinate by actinomycetes.
Racemic phenylsuccinate was stereospecifically degraded by the actinomycetes PS9 and PS17 isolated from soil obtained from Okinawa Island, Japan. Strain PS9, identified as a Citricoccus sp., preferentially degraded the R-form, while strain PS17, identified as a Microbacterium sp., preferentially degraded the S-form of phenylsuccinate. Analysis of the culture broths of these species with phenylsuccinate as the sole carbon source revealed that benzoic acid was produced as a metabolic intermediate. Benzoic acid was further degraded by strain PS9 with m- and/or p-hydroxybenzoic acid but not o-hydroxybenzoic acid as possible intermediates.
Matsui
Toru
T
Center of Molecular Biosciences, University of the Ryukyus, 1 Sembaru, Nishihara-cho, Okinawa 903-0213, Japan. tmatsui@comb.u-ryukyu.ac.jp
Kato
Katsuya
K
Namihira
Tomoyuki
T
Shinzato
Naoya
N
Semba
Hisashi
H
D039903:Actinobacteria / Q000145:classification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D010802:Phylogeny; D013237:Stereoisomerism; D013386:Succinates / Q000378:metabolism*
D013386:Succinates; C017625:2-phenylsuccinate