title: Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry.
abstract: An ultra fast liquid chromatography coupled with IT-TOF mass spectrometry (UFLC/MS-IT-TOF) metabonomic approach was employed to study the plasma and urine metabolic profiling of atherosclerosis rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the atherosclerosis and the control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass and MS(n) fragments information obtained from UFLC/MS-IT-TOF analysis. 12 metabolites in rat plasma and 8 metabolites in urine were identified as potential biomarkers. Concentrations of leucine, phenylalanine, tryptophan, acetylcarnitine, butyrylcarnitine, propionylcarnitine and spermine in plasma and 3-O-methyl-dopa, ethyl N2-acetyl-L-argininate, leucylproline, glucuronate, t6A N(6)-(N-threonylcarbonyl)-adenosine and methyl-hippuric acid in urine decreased in atherosclerosis rats. Ursodeoxycholic acid, chenodeoxycholic acid, LPC (C16:0), LPC (C18:0) and LPC (C18:1) in plasma and hippuric acid in urine were in higher levels in atherosclerosis rats. The alterated metabolites demonstrated abnormal metabolism of phenylalanine, tryptophan, bile acids and amino acids. This research proved that metabonomics is a promising tool for disease research.
authors: Department of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Fengxia
authors: F
authors: Zhang
authors: Zhenhua
authors: Z
authors: Jia
authors: Peng
authors: P
authors: Gao
authors: Hongwei
authors: H
authors: Kong
authors: Xiang
authors: X
authors: Li
authors: Jing
authors: J
authors: Chen
authors: Qin
authors: Q
authors: Yang
authors: Peiyuan
authors: P
authors: Yin
authors: Jiangshan
authors: J
authors: Wang
authors: Xin
authors: X
authors: Lu
authors: Famei
authors: F
authors: Li
authors: Yiling
authors: Y
authors: Wu
authors: Guowang
authors: G
authors: Xu
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000378:metabolism* / Q000652:urine; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid; D004041:Dietary Fats; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D015415:Biomarkers; D004041:Dietary Fats