Identification of cis-localization elements that target glutelin RNAs to a specific subdomain of the cortical endoplasmic reticulum in rice endosperm cells.
Rice glutelin RNAs are localized to the cisternal endoplasmic reticulum (ER) by a regulated RNA transport process requiring specific cis-localization elements. We set out to identify these glutelin sequences by their dominant character of being able to re-direct the normal protein body ER localization of a maize 10 kDa delta-zein RNA to the cisternal ER. In situ RNA localization analysis showed that the glutelin RNA contains multiple cis-localization elements; two located at the 5' and 3' ends of the coding sequences and a third located within the 3'-untranslated region. These three regions contain two conserved sequences, suggesting that these RNA recognition signals may be sequence based.
Washida
Haruhiko
H
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
Kaneko
Sachiyo
S
Crofts
Naoko
N
Sugino
Aya
A
Wang
Changlin
C
Okita
Thomas W
TW
D020413:3' Untranslated Regions / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005983:Glutens / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D034443:RNA Transport; D018749:RNA, Plant / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics / Q000378:metabolism; D015028:Zein / Q000378:metabolism
D020413:3' Untranslated Regions; D018749:RNA, Plant; D005983:Glutens; D015028:Zein