Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1.
Adiponectin has anti-atherosclerotic effects through its direct actions on vascular cells. The present study investigates the molecular mechanisms of adiponectin in the migration of endothelial progenitor cells (EPCs) which play an important role in neovascularization and re-endothelization. The phosphorylation of Akt and the activations of Cdc42 and Rac1 were significantly increased by adiponectin. Adiponectin increased the migration activity of EPCs, which was completely inhibited by a PI3-kinase inhibitor. siRNA of Cdc42 or Rac1 completely inhibited the adiponectin-induced migration, but siRNA of Akt had no effects, indicating that adiponectin promotes the migration activities of EPCs mainly through PI3-kinase/Cdc42/Rac1.
Nakamura
Nobuhisa
N
Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Naruse
Keiko
K
Matsuki
Takashi
T
Hamada
Yoji
Y
Nakashima
Eitaro
E
Kamiya
Hideki
H
Matsubara
Tatsuaki
T
Enomoto
Atsushi
A
Takahashi
Masahide
M
Oiso
Yutaka
Y
Nakamura
Jiro
J
D000199:Actins / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D004789:Enzyme Activation; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D000199:Actins; D052242:Adiponectin; D034741:RNA, Small Interfering; D054419:Receptors, Adiponectin; D042461:Vascular Endothelial Growth Factor A; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein