title: NADPH oxidase activity in allergenic pollen grains of different plant species.
abstract: Pollen is an important trigger of allergic diseases. Recent studies have shown that ragweed pollen NAD(P)H oxidase generates reactive oxygen species (ROS) and plays a prominent role in the pathogenesis of allergies in mouse models. Here, we demonstrated that allergenic pollen grains showed NAD(P)H oxidase activity that differed in intensity and localization according to the plant families. The activity occurred at the surface or in the cytoplasm in pollen of grasses, birch, and ragweed; in subpollen particles released from ragweed pollen; and at the inner surface or in the cytoplasm but not on the outer wall, which was sloughed off after the rupture, of pollen of Japanese cedar and Japanese cypress. The activity was mostly concentrated within insoluble fractions, suggesting that it facilitates the exposure of tissues to ROS generated by this enzyme. The extent of exposure to pollen-generated ROS could differ among the plant families.
authors: Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan.
authors: Xiao-Ling
authors: XL
authors: Wang
authors: Toshiro
authors: T
authors: Takai
authors: Seiji
authors: S
authors: Kamijo
authors: Hendra
authors: H
authors: Gunawan
authors: Hideoki
authors: H
authors: Ogawa
authors: Ko
authors: K
authors: Okumura
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D032485:Cryptomeria / Q000201:enzymology / Q000276:immunology; D029781:Cupressus / Q000201:enzymology / Q000276:immunology; D006967:Hypersensitivity / Q000201:enzymology / Q000276:immunology; D051379:Mice; D019255:NADPH Oxidases / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011058:Pollen / Q000201:enzymology* / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000485:Allergens; D017382:Reactive Oxygen Species; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase; D019255:NADPH Oxidases