Molecular cloning and functional analysis of polymeric immunoglobulin receptor gene in orange-spotted grouper (Epinephelus coioides).
As one of the most important mucosal effectors, polymeric immunoglobulin receptor (pIgR) mediates the transcytosis of polymeric immunoglobulins (pIgs) to protect the organisms. In this study, a full-length cDNA of pIgR was isolated from orange-spotted grouper (Epinephelus coioides), and the sequence analysis of deduced protein revealed the presence of only two Ig-like domains (ILDs), and the absence of the conserved Ig-binding site and complementary determining region (CDR). The grouper pIgR mRNA was detected in almost all the peripheral tissues examined, especially the mucosal tissues by RT-PCR. Additionally, recombinant grouper pIgR was stably expressed in the COS-7 cell line and identified as a 40-kDa transmembrane receptor. Furthermore, the association of recombinant pIgR and purified grouper pIgM was demonstrated. Taken together, the present study provided strong evidence that grouper pIgR was produced as a transmembrane protein, and probably involved in the pIgM transport.
Feng
Li-Na
LN
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, PR China.
Lu
Dan-Qi
DQ
Bei
Jin-Xin
JX
Chen
Jie-Lin
JL
Liu
Yun
Y
Zhang
Yong
Y
Liu
Xiao-Chun
XC
Meng
Zi-Ning
ZN
Wang
Lei
L
Lin
Hao-Ran
HR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D023281:Genomics; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D019056:Receptors, Polymeric Immunoglobulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D007075:Immunoglobulin M; D019056:Receptors, Polymeric Immunoglobulin; D011994:Recombinant Proteins