title: Comparisons between geographically diverse samples of carried Staphylococcus aureus.
abstract: Approximately one-third of the human population is asymptomatically colonized by Staphylococcus aureus. However, much of the global diversity within the carriage populations remains uncharacterized, and it is unclear to what degree the variation is geographically partitioned. We isolated 300 carriage isolates from 1,531 adults contemporaneously in four countries: France, Algeria, Moldova, and Cambodia. All strains were characterized by multilocus sequence typing. Six clonal complexes (CCs) were present in all four samples (CC30, -45, -121, -15, -5, and -8). Analyses based on the genotype frequencies revealed the French and Algerian samples to be most similar and the Cambodian sample to be most distinct. While this pattern is consistent with likely rates of human migration and geographic distance, stochastic clonal expansion also contributes to regional differences. Phylogenetic analysis revealed a highly divergent and uncharacterized genotype (ST1223) within Cambodia. This lineage is related to CC75, which has previously been observed only in remote aboriginal populations in northern Australia.
authors: Laboratoire Associé Centre National de Référence Résistance dans les Flores Commensales, Hôpital Bichat-Claude Bernard (AP-HP), 75018 Paris, France. raymond.ruimy@bch.ap-hop-paris.fr
authors: Raymond
authors: R
authors: Ruimy
authors: Laurence
authors: L
authors: Armand-Lefevre
authors: Francois
authors: F
authors: Barbier
authors: Etienne
authors: E
authors: Ruppé
authors: Radu
authors: R
authors: Cocojaru
authors: Yasmine
authors: Y
authors: Mesli
authors: Aminata
authors: A
authors: Maiga
authors: Mokhtar
authors: M
authors: Benkalfat
authors: Samia
authors: S
authors: Benchouk
authors: Hafida
authors: H
authors: Hassaine
authors: Jean-Baptiste
authors: JB
authors: Dufourcq
authors: Chhor
authors: C
authors: Nareth
authors: Jean-Louis
authors: JL
authors: Sarthou
authors: Antoine
authors: A
authors: Andremont
authors: Edward J
authors: EJ
authors: Feil
mesh_terms: D000328:Adult; D000462:Algeria / Q000453:epidemiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002160:Cambodia / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008966:Moldova / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial