Biofunctionalized tilted Fiber Bragg Gratings for label-free immunosensing.
We present a study aimed at developing a label-free optical fiber biosensor for detection and quantification of biomolecules in real-time. The biosensor based on a Tilted Fiber Bragg Grating (TFBG) transduces a binding event between the probe and target molecules into a change in the refractive index of the medium surrounding the fiber. This work describes the experimental results obtained with three methods for immobilizing biomolecular probes on a TFBG silica cladding surface. Bovine serum albumin (BSA) and anti-BSA are used to assess the performances of the TFBG based biosensor in each configuration.
Maguis
Séverine
S
CEA, LIST, Laboratoire de Mesures Optiques, Centre d'Etudes de Saclay, Gif-sur-Yvette cedex, France. severine.maguis@cea.fr
Laffont
Guillaume
G
Ferdinand
Pierre
P
Carbonnier
Benjamin
B
Kham
Khémara
K
Mekhalif
Tahar
T
Millot
Marie-Claude
MC
D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013194:Staining and Labeling
D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine