The Arabidopsis 26S proteasome subunit RPN1a is required for optimal plant growth and stress responses.
The current literature offers contradictory results regarding the role of the proteasome subunit RPN1a in Arabidopsis development. Here we show that plants lacking RPN1a are viable and have increased cell sizes, decreased heat shock tolerance, increased oxidative stress tolerance and other phenotypes characteristic for 26S proteasome subunit mutants. These results strengthen our contention that most of the phenotypes of 26S proteasome mutants in Arabidopsis described to date reflect a general impairment in 26S proteasome function rather than a specific defect of a single subunit, and suggest that the role of the RPN1a subunit during embryogenesis needs to be reconsidered.
Wang
Songhu
S
Plant Physiology, Biochemistry, Molecular Biology Program, Department of Plant and Soil Sciences, College of Agriculture, University of Kentucky, Lexington, KY 40546, USA.
Kurepa
Jasmina
J
Smalle
Jan A
JA
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D016254:Mutagenesis, Insertional; D009154:Mutation; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D013312:Stress, Physiological
D029681:Arabidopsis Proteins; D021122:Protein Subunits; C507997:RPN1 protein, Arabidopsis; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease