Stenotrophomonas maltophilia genomes: a start-up comparison.
The whole DNA sequences of 2 Stenotrophomonas maltophilia strains isolated from the blood of a cancer patient (K279a) and the poplar Populus trichocarpa (R551-3) have been compared. The 2 chromosomes exhibit extensive synteny, but each is punctuated by about 40 genomic islands (GEIs), which vary in size from 3 to 70kb, and may encode up to about 50 proteins. A large set of smaller DNA sequences, encoding strain-specific 'solo' orfs, contributes to genetic heterogeneity in a significant manner. S. maltophilia GEIs potentially encode several proteins mediating interactions with the environment such as transmembrane proteins, haemagglutinins, components of type I and IV secretion systems, and efflux proteins having a role in metal and/or drug resistance. The presence of specific GEIs in the S. maltophilia population was monitored by PCR and slot-blot analyses. Data suggest that some islands are present at sites different from those identified in K279a and that alternative islands may be integrated at mapped sites.
Rocco
Francesco
F
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università Federico II, 80131 Napoli, Italy.
De Gregorio
Eliana
E
Colonna
Bianca
B
Di Nocera
Pier Paolo
PP
D016470:Bacteremia / Q000382:microbiology; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D044404:Genomic Islands; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D032107:Populus / Q000382:microbiology; D020615:Stenotrophomonas maltophilia / Q000235:genetics* / Q000302:isolation & purification; D026801:Synteny
D004269:DNA, Bacterial