Lactobacillus taiwanensis sp. nov., isolated from silage.
Strain FIRDI 006T, isolated from silage cattle feed, was characterized by using phenotypic and molecular taxonomic methods. The strain was a homofermentative lactic acid bacterium; the cells stained Gram-positive and were catalase-negative, non-motile, facultatively anaerobic rods. 16S rRNA gene sequence analysis revealed that the strain belongs phylogenetically to the genus Lactobacillus and can be placed within the Lactobacillus acidophilus-delbrueckii group. Lactobacillus gasseri and Lactobacillus johnsonii were the most closely related species, with 16S rRNA gene sequence similarities of 99.53 and 99.46% to the respective type strains. Low gyrB gene sequence similarities (<90%) and low DNA-DNA reassociation values (<45%) were obtained between the strain and the phylogenetically closest neighbours. Based on phenotypic and genetic evidence, the strain is considered to represent a novel species, for which the name Lactobacillus taiwanensis sp. nov. is proposed. The type strain is FIRDI 006T (=BCRC 17755T =DSM 21401T).
Wang
Li-Ting
LT
Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30099, Taiwan, ROC.
Kuo
Hsiao-Ping
HP
Wu
Yen-Chi
YC
Tai
Chun-Ju
CJ
Lee
Fwu-Ling
FL
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012820:Silage / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002374:Catalase; D027081:DNA Gyrase