Characterization of mundticin L, a class IIa anti-Listeria bacteriocin from Enterococcus mundtii CUGF08.
Enterococcus mundtii CUGF08, a lactic acid bacterium isolated from alfalfa sprouts, was found to produce mundticin L, a new class IIa bacteriocin that has a high level of inhibitory activity against the genus Listeria. The plasmid-associated operons containing genes for the mundticin L precursor, the ATP binding cassette (ABC) transporter, and immunity were cloned and sequenced. The fifth residue of the conservative consensus sequence YGNGX in the mature bacteriocin is leucine instead of valine in the sequences of the homologous molecules mundticin KS (ATO6) and enterocin CRL35. The primary structures of the ABC transporter and the immunity protein are homologous but unique.
Feng
Guoping
G
Department of Food Science and Technology, Cornell University, Geneva, NY 14456, USA.
Guron
Giselle K P
GK
Churey
John J
JJ
Worobo
Randy W
RW
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001430:Bacteriocins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016983:Enterococcus / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D008087:Listeria / Q000187:drug effects*; D000455:Medicago sativa / Q000382:microbiology; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; C114588:mundticin