AQP1 is not only a water channel: it contributes to cell migration through Lin7/beta-catenin.
BACKGROUND
AQP1 belongs to aquaporins family, water-specific, membrane-channel proteins expressed in diverse tissues. Recent papers showed that during angiogenesis, AQP1 is expressed preferentially by microvessels, favoring angiogenesis via the increase of permeability In particular, in AQP1 null mice, endothelial cell migration is impaired without altering their proliferation or adhesion. Therefore, AQP1 has been proposed as a novel promoter of tumor angiogenesis.


RESULTS
Using targeted silencing of AQP1 gene expression, an impairment in the organization of F-actin and a reduced migration capacity was demonstrated in human endothelial and melanoma cell lines. Interestingly, we showed, for the first time, that AQP1 co-immunoprecipitated with Lin-7. Lin7-GFP experiments confirmed co-immunoprecipitation. In addition, the knock down of AQP1 decreased the level of expression of Lin-7 and beta-catenin and the inhibition of proteasome contrasted partially such a decrease.


CONCLUSIONS
All together, our findings show that AQP1 plays a role inside the cells through Lin-7/beta-catenin interaction. Such a role of AQP1 is the same in human melanoma and endothelial cells, suggesting that AQP1 plays a global physiological role. A model is presented.
Monzani
Elena
E
Department of Biomolecular Science and Biotechnology, University of Milan, Milan, Italy.
Bazzotti
Riccardo
R
Perego
Carla
C
La Porta
Caterina A M
CA
D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins; D051379:Mice; D018345:Mice, Knockout; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033921:Vesicular Transport Proteins; D051176:beta Catenin / Q000502:physiology*
C496021:Aqp1 protein, mouse; D002352:Carrier Proteins; C114670:Lin7a protein, mouse; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins; D051176:beta Catenin; D051398:Aquaporin 1