Fluorescent biological label for recognizing human ovarian tumor cells based on fluorescent nanoparticles.
In this paper, we report a method for recognizing human ovarian tumor (HOT) cells using fluorescent biological label based on core-shell nanoparticles. The luminescent nanoparticles were synthesized with a water-in-oil (W/O) microemulsion technique. The fluorescent silica core-shell nanoparticles modified with anti-HER2 antibody using bifunctional cross-linker glutaraldehyde targeted the corresponding tumor antigen in the cell surface of the SKOV3 ovarian cancer cells. The specific immunoreactivity of antibody-nanoparticles with cells was characterized by laser scanning microscopy (LSM) and scanning electron microscope (SEM). The results showed that the method offered potential advantages of sensitivity and simplicity due to high binding efficiency between nanoparticles and cells and provided an alternative method for the detection of HOT.
Huang
Shasheng
S
College of Life and Environment, Shanghai Normal University, Shanghai 200234, China. sshuang@shnu.edu.cn
Li
Ruina
R
Qu
Yongxia
Y
Shen
Jian
J
Liu
Jia
J
D001323:Autoantibodies; D045744:Cell Line, Tumor; D005260:Female; D005453:Fluorescence; D005976:Glutaral; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence
D001323:Autoantibodies; D005976:Glutaral; D018719:Erb-b2 Receptor Tyrosine Kinases; D012822:Silicon Dioxide; C508053:ERBB2 protein, human