title: An improved method for staining kinetochores of human chromosomes.
abstract: An improved method, which exhibited simultaneously clearly kinetochores and the nucleolar organizer regions of human chromosomes by pretreating of human metaphase chromosomes with HCl and NaOH, followed by staining with silver nitrate and visualizing using ammoniacal silver, is described in the present communication. It has important role for analysis of kinetochore variation, mechanism of chromosomal non-disjunction as well as identification of fuctional active centromeres.
authors: Department of Genetics, No.1 Yixueyuan Road, Yuzhong Street, Chongqing 400016, People's Republic of China.
authors: Junlin
authors: J
authors: He
authors: Xueqing
authors: X
authors: Liu
authors: Yubin
authors: Y
authors: Ding
authors: Chao
authors: C
authors: Yu
authors: Yaguang
authors: Y
authors: Weng
authors: Xuemei
authors: X
authors: Chen
authors: Rufei
authors: R
authors: Gao
authors: Yingxiong
authors: Y
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D002877:Chromosomes, Human; D006801:Humans; D018386:Kinetochores; D008677:Metaphase; D013194:Staining and Labeling / Q000379:methods*