title: Natronovirga wadinatrunensis gen. nov., sp. nov. and Natranaerobius trueperi sp. nov., halophilic, alkalithermophilic micro-organisms from soda lakes of the Wadi An Natrun, Egypt.
abstract: Novel strains of anaerobic, halophilic, alkalithermophilic bacteria were isolated from sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Strains JW/NM-WN-LUT (and related strains) and JW/NM-WN-LH1T were non-spore-forming, non-motile bacteria with a Gram-type positive cell wall. Optimal growth of strain JW/NM-WN-LUT occurred at pH55 degrees C 9.5, 52 degrees C and with 3.7 M Na+ (2.2 M added NaCl). Optimal growth of strain JW/NM-WN-LH1T occurred at pH55 degrees C 9.9, 51 degrees C and with 3.9 M Na+ (2.3 M added NaCl). Both strains were obligately anaerobic and chemoorganotrophic; producing lactate and acetate as organic acids from pyruvate in different ratios. The DNA G+C contents of strains JW/NM-WN-LUT and JW/NM-WN-LH1T were 41.0 and 42.0 mol%, respectively. The main cellular fatty acids in both strains were anteiso-branched 15:0 and iso-branched 15:0. Phylogenetic analysis showed that both novel isolates belonged to the family Natranaerobiaceae within the order Natranaerobiales. Based on genotypic and phenotypic data, strain JW/NM-WN-LUT (=DSM 18760T=ATCC BAA-1443T) represents the type strain of a novel species, Natranaerobius trueperi sp. nov. Strain JW/NM-WN-LH1T represents a novel genus and species within the family Natranaerobiaceae, Natronovirga wadinatrunensis gen. nov., sp. nov. The type strain of the type species is JW/NM-WN-LH1T (=DSM 18770T=ATCC BAA-1444T).
authors: Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
authors: Noha M
authors: NM
authors: Mesbah
authors: Juergen
authors: J
authors: Wiegel
mesh_terms: D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004534:Egypt; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006358:Hot Temperature; D007773:Lactates / Q000378:metabolism; D008124:Locomotion; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D011773:Pyruvates / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013171:Spores, Bacterial / Q000166:cytology
chemical_list: D000085:Acetates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D007773:Lactates; D011773:Pyruvates; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride