Interaction between interferon regulatory factor-1 and human papillomavirus E7 oncogene in cervical cancer: an ontology study.
OBJECTIVE
Cervical cancer is an important female malignancy. The discovery of human papillomavirus (HPV) as an etiologic agent of cervical cancer has prompted increased interest in the biology and oncogenicity of this virus. The E7 protein is found predominantly in the nucleus and, to a lesser extent, in the cytoplasm in cervical cancer cell lines. HPV E7 has been shown to be functionally associated with the tumor suppressor interferon regulatory factor (IRF)-1 in cervical carcinogenesis.


METHODS
In this study, new gene ontology technology was used to predict changes in the molecular function and biologic processes caused by the interaction between IRF-1 and HPV E7.


RESULTS
The molecular function and biologic processes of IRF-1 and the combined IRF-1 and HPV E7 (IRF-1-E7) were derived using the GoFigure server. The combined IRF-1-E7 demonstrated more functions and biologic processes compared with IRF-1 alone.


CONCLUSIONS
IRF-1-E7 was shown to be responsible for the positive regulation of many interleukins and to be involved in the differentiation of T-helper cells.
Wiwanitkit
Viroj
V
Wiwanitkit House, Bangkhae, Bangkok, Thailand. wviroj@yahoo.com
D002454:Cell Differentiation / Q000276:immunology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D030541:Databases, Genetic; D005260:Female; D006801:Humans; D050856:Interferon Regulatory Factor-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007372:Interferons / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000821:virology*
D004268:DNA-Binding Proteins; C052633:E7 protein, Human papillomavirus type 18; C494257:IRF1 protein, human; D050856:Interferon Regulatory Factor-1; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D007372:Interferons