title: UVB radiation induces persistent activation of ribosome and oxidative phosphorylation pathways.
abstract: Ultraviolet B (UVB) radiation has strong biological effects and modulates the expression of many genes. The major biological pathways affected by UVB radiation remain controversial. In this work, we used a loop-design microarray approach and applied rigorous statistical analyses to identify differentially regulated genes at 4, 8, 16 or 24 h after UVB irradiation. The most prominent biological categories in lists of differentially regulated gene sets were extracted by functional enrichment analysis. With this approach, we determined that genes participating in two prime cellular processes, the ribosome pathway and the oxidative phosphorylation pathway, were persistently activated after UVB irradiation. Mitochondrial activity assays confirmed increased activity for up to 24 h after UVB irradiation. These results suggest that the persistent activation of ribosome and oxidative phosphorylation pathways may have a key role in UVB-radiation-induced cellular responses. For the first time, the specific cellular pathways that respond to UVB radiation consistently and persistently can be delineated with confidence using a loop-design microarray approach and functional bioinformatics analysis. The results of this study offer further insight into UVB-radiation-induced stress responses.
authors: Department of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
authors: Min-Lung
authors: ML
authors: Tsai
authors: Kun-Yu
authors: KY
authors: Chang
authors: Chi-Shiun
authors: CS
authors: Chiang
authors: Wun-Yi
authors: WY
authors: Shu
authors: Tsai-Chun
authors: TC
authors: Weng
authors: Chaang Ray
authors: CR
authors: Chen
authors: Ching-Lung
authors: CL
authors: Huang
authors: Hua-Kuo
authors: HK
authors: Lin
authors: Ian C
authors: IC
authors: Hsu
mesh_terms: D002460:Cell Line; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects; D020411:Oligonucleotide Array Sequence Analysis; D010085:Oxidative Phosphorylation / Q000528:radiation effects*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D013997:Time Factors; D014466:Ultraviolet Rays