title: Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to hypoxic stress.
abstract: Hypoxia, as one suboptimal environmental condition, can affect the physiological state of shrimp during pond aquaculture. To better understand the mechanism of response to hypoxic stress in Chinese shrimp Fenneropenaeus chinensis, proteome research approach was utilized. Differentially expressed proteins of hepatopancreas in adult Chinese shrimp between the control and hypoxia-stressed groups were screened. By 2-DE analysis, 67 spots showed obvious changes after hypoxia. Using LC-ESI-MS/MS, 51 spots representing 33 proteins were identified including preamylase, arginine kinase, phosphopyruvate hydratase, citrate synthase, ATP synthase alpha subunit, chymotrypsin BI, chitinase, ferritin, C-type lectin receptors, transketolase, formylglutathione hydrolase, formyltetrahydrofolate dehydrogenase, aldehyde dehydrogenase, glutathione peroxidase, cytosolic manganese superoxide dismutase, protein disulfide isomerase, beta-actin, oncoprotein nm23, crustacyanin-C1 and so on. These proteins could be functionally classified into several groups such as proteins related to energy production, metabolism-related proteins, immune-related proteins, antioxidant proteins, chaperones, cytoskeleton proteins and ungrouped proteins. The transcription levels of ten selected genes encode the identified proteins were analyzed by real-time PCR at different sampling times of hypoxia. This study is the first analysis of differentially expressed proteins in the hepatopancreas of shrimp after hypoxia and provides a new insight for further study in hypoxic stress response of shrimp at the protein level.
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, PR China.
authors: Hao
authors: H
authors: Jiang
authors: Fuhua
authors: F
authors: Li
authors: Yusu
authors: Y
authors: Xie
authors: Bingxin
authors: B
authors: Huang
authors: Jinkang
authors: J
authors: Zhang
authors: Jiquan
authors: J
authors: Zhang
authors: Chensong
authors: C
authors: Zhang
authors: Shihao
authors: S
authors: Li
authors: Jianhai
authors: J
authors: Xiang
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D033561:Penaeidae / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D060829:Arthropod Proteins; D020543:Proteome; D010100:Oxygen