title: Construction of a protein library of Arabidopsis transcription factors using a wheat cell-free protein production system and its application for DNA binding analysis.
abstract: We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5'-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.
authors: Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University, Matsuyama, Ehime, Japan.
authors: Akira
authors: A
authors: Nozawa
authors: Yuko
authors: Y
authors: Matsubara
authors: Yoshinori
authors: Y
authors: Tanaka
authors: Hirotaka
authors: H
authors: Takahashi
authors: Tatsuya
authors: T
authors: Akagi
authors: Motoaki
authors: M
authors: Seki
authors: Kazuo
authors: K
authors: Shinozaki
authors: Yaeta
authors: Y
authors: Endo
authors: Tatsuya
authors: T
authors: Sawasaki
mesh_terms: D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000096:biosynthesis / Q000378:metabolism; D002474:Cell-Free System; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019151:Peptide Library; D014157:Transcription Factors / Q000096:biosynthesis* / Q000378:metabolism*; D014908:Triticum / Q000166:cytology / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D019151:Peptide Library; D014157:Transcription Factors; D004247:DNA