title: Oral administration of lactic acid bacteria isolated from traditional South Asian fermented milk 'dahi' inhibits the development of atopic dermatitis in NC/Nga mice.
abstract: We investigated the suppressive effect of lactic acid bacteria (LAB) isolated from traditional South Asian fermented milk 'dahi' on the development of atopic dermatitis (AD) using NC/Nga AD model mice. In the initial evaluation, we confirmed the effect of LAB on serum total IgE using ovalbumin (OVA)-induced type 1 allergy model mice. Forty-one bacterial strains isolated from dahi were evaluated for their ability to induce interleukin (IL)-12 production and suppress IL-4 production in splenocytes obtained from OVA-sensitized mice. Of the 41 strains tested, Lactobacillus delbrueckii subsp. lactis R-037 exhibited the greatest IL-12 induction, suggesting that it is a potent Th1 inducer. Oral administration of heat-treated R-037 significantly suppressed the elevation of serum total IgE in OVA-induced type 1 allergy model mice. In NC/Nga AD model mice, oral administration of heat-treated R-037 reduced inflammatory auricular thickness and alleviated the AD clinical score although the effect on serum total IgE level was unclear. Histopathological findings showed a tendency toward improvement of inflammation. Hyperkeratosis in particular showed improvement in dermatitis skin lesions. These results suggest that oral administration of R-037 may alleviate AD.
authors: Frontier Biochemical and Medical Research Laboratories, Corporate Research and Development Division, Kaneka Corporation, Takasago, Hyogo, Japan. Toyoaki_Watanabe@kn.kaneka.co.jp
authors: Toyoaki
authors: T
authors: Watanabe
authors: Kazuya
authors: K
authors: Hamada
authors: Airo
authors: A
authors: Tategaki
authors: Hideyuki
authors: H
authors: Kishida
authors: Hozumi
authors: H
authors: Tanaka
authors: Mitsuaki
authors: M
authors: Kitano
authors: Taku
authors: T
authors: Miyamoto
mesh_terms: D000818:Animals; D043302:Cultured Milk Products / Q000382:microbiology*; D003876:Dermatitis, Atopic / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D018664:Interleukin-12 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007778:Lactobacillus / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D012867:Skin / Q000473:pathology; D013154:Spleen / Q000276:immunology
chemical_list: D018664:Interleukin-12; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin