title: MK801-induced activated caspase-3 exhibits selective co-localization with GAD67.
abstract: Blockade of the N-methyl-d-aspartate receptor (NMDAR) in postnatal day 7 (P7) rats can promote rapid and robust induction of the pro-apoptotic marker activated caspase-3 (AC3) and loss of the GABAergic marker GAD67 at P56. Thus, we hypothesized that NMDAR blockade-induced AC3 occurs in GAD67 positive cells at P7. To test this idea, we injected P7 rat pups with vehicle or MK801 and after 8h (peak of AC3 induction) we examined brain sections for both AC3 and GAD67. Compared to vehicle, MK801 profoundly induced AC3 in all brain regions examined but co-expression of GAD67 in the same cells was not observed. However, in brain regions where punctate (synaptic) GAD67 was abundant (for example, layer IV of the somatosensory cortex), AC3 was robust. These data suggest that whereas somatic expression of AC3 and GAD67 may be non-overlapping, areas that exhibit punctate GAD67 (and are high in synaptic turnover) may be more vulnerable to MK801 exposure.
authors: Neurobiology and Anatomy, Wake Forest University School of Medicine, 1 Medical Center Blvd, Winston-Salem, NC 27157-1010, United States. cpturner@wfubmc.edu
authors: Christopher P
authors: CP
authors: Turner
authors: Danielle
authors: D
authors: Debenedetto
authors: Emily
authors: E
authors: Ware
authors: Caroline
authors: C
authors: Walburg
authors: Andrew
authors: A
authors: Lee
authors: Robert
authors: R
authors: Stowe
authors: John
authors: J
authors: Swanson
authors: Alexander
authors: A
authors: Lambert
authors: Melissa
authors: M
authors: Lyle
authors: Priyanka
authors: P
authors: Desai
authors: Raymond
authors: R
authors: Johnson
authors: Chun
authors: C
authors: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005968:Glutamate Decarboxylase / Q000187:drug effects / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats
chemical_list: D018691:Excitatory Amino Acid Antagonists; D016291:Dizocilpine Maleate; D053148:Caspase 3; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1