The immunohistochemical demonstration of noradrenergic neurons in the rat brain: The use of homologous antiserum to dopamine-beta-hydroxylase.
The immunohistochemical localization of dopamine-beta-hydroxylase (DBH) in central noradrenergic neurons of the rat is greatly improved by the use of homologous antiserum (i.e. antibody against purified rat DBH). This antiserum, which has a several thousand-fold greater affinity for rat DBH than does heterologous antibody, is used at a 1:500 dilution with low concentrations of detergent. This method applied to paraformaldehyde-perfused tissue consistently yields highly sensitive and selective staining with excellent cytologic preservation of fine neuronal processes.
Grzanna
R
R
Departments of Anatomy, Neurology, Pharmacology and Psychiatry, The Johns Hopkins University School of Medicine, Baltimore, Md. 21205, U.S.A.
Morrison
J H
JH
Coyle
J T
JT
Molliver
M E
ME