[A pilot study on hypoxia of squamous cell carcinoma in oral cavity].
OBJECTIVE
To examine the extent of hypoxia in oral squamous cell carcinoma and investigate the factors related to the hypoxia.


METHODS
An animal model of oral squamous cell carcinoma was established and single photon emission computed tomography (SPECT) with 99mTc-HL91 used to detect the hypoxia extent of the oral squamous cell carcinoma. The expression of hypoxia inducible factor 1 alpha (HIF-1alpha) was examined by immunohistochemical staining in 42 cases of formalin-fixed paraffin-embed squamous cell carcinoma.


RESULTS
The uptake of 99mTc-HL91 in the tumor tissue was higher than that in normal tissue and had linear relation with the tumor size (P < 0.05). There was no HIF-1alpha expression in the normal oral mucosa. The expression of HIF-1alpha was high in oral mucosa carcinoma and closely related to the differentiation degree of tumor and metastasis of lymph node (P < 0.05).


CONCLUSIONS
Tumor tissue had broad hypoxic region. HIF-1alpha highly expressed in oral squamous cell carcinoma and may play an important role in carcinogenesis and aggression.
Liu
Wen-Shu
WS
Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Shang
Jian-Wei
JW
Gao
Yan
Y
Guo
Chuan-Bin
CB
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D010865:Pilot Projects; D015899:Tomography, Emission-Computed, Single-Photon; D023041:Xenograft Model Antitumor Assays
D051795:Hypoxia-Inducible Factor 1, alpha Subunit