Benzodiazepine receptor binding in rat spinal cord membranes.
Using [(3)H]diazepam, benzodiazepine receptor binding has been investigated in rat spinal cord synaptosomal membranes. Specific [(3)H]diazepam binding is saturable with a K(D) of about 8 nM and a maximal number of binding sites of 0.48 pmol/mg protein, which is about half of the density found in rat brain synaptosomal membranes. Using 5 benzodiazepine derivatives and amino acid neurotransmitters and their antagonists, no differences in specific [(3)H]diazepam binding to rat spinal cord and rat brain synaptosomal membranes were found. The results do not support the hypothesis that benzodiazepines act via glycine receptors in the spinal cord.
Müller
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Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, D-6500 Mainz, G.F.R.
Schläfer
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Wollert
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