title: The effect of caffeic acid phenethyl ester on the functions of human monocyte-derived dendritic cells.
abstract: BACKGROUND
  Propolis, an ancient herbal medicine, has been reported the beneficial effect both in asthma patients and murine model of asthma, but the mechanism was not clearly understood. In this study, the effect of caffeic acid phenethyl ester (CAPE), the most extensively studied components in propolis, on the functions of human monocyte-derived dendritic cells (MoDCs) was investigated.
  
  
  RESULTS
  CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract. CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract. In contrast, the upregulation of costimulatory molecules in mature MoDCs was not suppressed by CAPE. Further, the antigen presenting ability of DCs was not inhibited by CAPE. CAPE inhibited IkappaBalpha phosphorylation and NF-kappaB activation but not mitogen-activated protein kinase (MAPK) family phosphorylation in human MoDCs.
  
  
  CONCLUSIONS
  These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway. This study provided a new insight into the mechanism of CAPE in immune response and the rationale for propolis in the treatment of asthma and other allergic disorders.
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan, Republic of China.lcwang5@ntu.edu.tw
authors: Li-Chieh
authors: LC
authors: Wang
authors: Yu-Li
authors: YL
authors: Lin
authors: Yu-Chih
authors: YC
authors: Liang
authors: Yao-Hsu
authors: YH
authors: Yang
authors: Jyh-Hong
authors: JH
authors: Lee
authors: Hsin-Hui
authors: HH
authors: Yu
authors: Wen-Mein
authors: WM
authors: Wu
authors: Bor-Luen
authors: BL
authors: Chiang
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D054357:Chemokine CXCL10 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D051550:I-kappa B Kinase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D053762:Interleukin-12 Subunit p40 / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000276:immunology / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects / Q000276:immunology; D011429:Propolis / Q000494:pharmacology*; D039981:Pyroglyphidae / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D000485:Allergens; C114753:CXCL10 protein, human; D002109:Caffeic Acids; D054357:Chemokine CXCL10; D053762:Interleukin-12 Subunit p40; D008070:Lipopolysaccharides; D016328:NF-kappa B; D016753:Interleukin-10; D018664:Interleukin-12; D011429:Propolis; D051550:I-kappa B Kinase; D020929:Mitogen-Activated Protein Kinase Kinases; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol