Development of a chromatographic strip assay for detection of porcine antibodies to 3ABC non-structural protein of foot-and-mouth disease virus serotype O.
A chromatographic strip assay was developed for rapid detection of serum antibodies to non-structural protein of foot-and-mouth disease virus. The assay was based on Escherichia coli-expressed 3ABC non-structural protein and an immunochromatographic technique, which shortened the detection time to about one hour. The sensitivity of the assay was determined to be 96.8% for infected pigs; its specificity was 100% for naïve pigs and 98.8% for vaccinated pigs. In the experimentally infected pigs, anti-3ABC antibodies were detectable from eight days post-infection until the end of the study, 34 days post-infection. The performance of this assay was comparable to that of two commercial ELISA kits, Ceditest FMDV-NS and UBI FMDV NS EIA, and was better than that of CHEKIT FMD-3ABC po. Given its advantages of instant testing and quantitative measurement, this assay has potential as a useful tool for rapid on-farm diagnosis of foot-and-mouth disease.
Chen
Tsu-Han
TH
Division of Hog Cholera Research, Animal Health Research Institute 376 Chung-Cheng Road, Tamsui, Taipei County 25158, Taiwan.
Pan
Chu-Hsiang
CH
Jong
Ming-Hwa
MH
Lin
Hsiu-Min
HM
Huang
Yu-Liang
YL
Hsiung
Kuang-Pin
KP
Chao
Parn-Hwa
PH
Lee
Fan
F
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011934:Reagent Strips; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
C109754:3ABC protein, virus; D000914:Antibodies, Viral; D012367:RNA, Viral; D011934:Reagent Strips; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins