[Effects of Liangxue Jiedu Decoction in treating psoriasis in a mouse psoriasis model].
OBJECTIVE
To study the effects of Liangxue Jiedu Decoction, a compound traditional Chinese herbal medicine with the function of blood-cooling and detoxicating, in treating psoriasis in mice and to explore its mechanism.


METHODS
(1) Sixty mice were randomly divided into Liangxue Jiedu Decoction group, compound Indigo Naturalis capsule group, acitretin capsule group and normal saline group. Another 10 mice were selected as blank control. After 2-week administration, mice were sacrificed to obtain samples. After hematoxylin and eosin (HE) staining, tail scales with granular layers were calculated by an optical microscope. (2) Except for ten mice in blank group, sixty female mice were injected intraperitoneally with diethylstilbestrol once daily. After 3-day injection, mice were randomly divided into four groups and treated as above description. After 2-week treatment, all mice were injected intraperitoneally with colchicine (2 mg/kg), and sacrificed 6 h after the injection. The mitotic rate in virginal epithelium was calculated after HE staining.


RESULTS
Compared with normal saline, Liangxue Jiedu Decoction could significantly inhibit the mitosis of mouse vaginal epithelium (P < 0.01) and promote the formation of granular layers in mouse tail-scale epidermis (P < 0.01).


CONCLUSIONS
The mechanism of Liangxue Jiedu Decoction in treating psoriasis may be related to promoting granular cell growth and inhibiting proliferation of epidermic cells.
Gu
Min-Jie
MJ
Department of Dermatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. guminjie1974@126.com
Gao
Shang-Pu
SP
Li
Yong-Mei
YM
D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D008517:Phytotherapy; D011565:Psoriasis / Q000188:drug therapy* / Q000473:pathology; D011897:Random Allocation; D014621:Vagina / Q000473:pathology
D004365:Drugs, Chinese Herbal