Psychrobacter lutiphocae sp. nov., isolated from the faeces of a seal.
The taxonomic status of a Gram-negative-staining bacterium, isolated from the faeces of a seal, was investigated using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that the novel isolate formed a distinct phyletic line within the genus Psychrobacter, displaying >3.3% sequence divergence with other known Psychrobacter species. The generic assignment was confirmed by chemotaxonomic data, which revealed a fatty acid profile that included straight-chain saturated, unsaturated and 3-hydroxylated fatty acids, with C18:1omega9c as the major fatty acid. A ubiquinone with eight isoprene units (Q-8) was the predominant respiratory quinone and spermidine was the predominant polyamine. The novel isolate was distinguished from other members of the genus Psychrobacter by using a set of phenotypic properties. On the basis of phenotypic and phylogenetic considerations, it is proposed that the new isolate represents a novel species, for which the name Psychrobacter lutiphocae sp. nov. is proposed. Strain IMMIB L-1110T (=DSM 21542T=CCUG 56590T) is the type strain.
Yassin
A F
AF
Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany. yassin@mibi03.meb.uni-bonn.de
Busse
H-J
HJ
D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D044096:Psychrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D046048:Seals, Earless / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011073:Polyamines; D011809:Quinones; D012336:RNA, Ribosomal, 16S