Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration.
The interactions between two oxidoreductases coupled by an artificial redox mediator have been described quantitatively to increase both stability and productivity. In this cascade oxidation, pyranose 2-oxidase oxidizes several aldoses at the C-2 position to 2-ketoaldoses. A redox mediator is used as electron acceptor for pyranose 2-oxidase because it shows more favourable kinetics in comparison to oxygen. The reduced redox mediator is in turn re-oxidized by laccase, which uses oxygen as the terminal electron acceptor, reducing it fully to water. However, pyranose 2-oxidase is capable of using oxygen as an electron acceptor in a competing side reaction, leading to the formation of hydrogen peroxide, which is detrimental for both enzymes and seriously limits the operational stability of both enzymes. The experimental results showed full conversion of the aldose to the 2-ketoaldose and a good agreement with the simulations of the process.
Van Hecke
Wouter
W
Research Group Environmental Organic Chemistry and Technology, Department of Organic Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Salaheddin
Clara
C
Ludwig
Roland
R
Dewulf
Jo
J
Haltrich
Dietmar
D
Van Langenhove
Herman
H
D000327:Adsorption; D016227:Benzoquinones / Q000737:chemistry; D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry*; D002606:Charcoal / Q000737:chemistry; D002621:Chemistry / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D042845:Laccase / Q000737:chemistry*; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*
D016227:Benzoquinones; D002606:Charcoal; C004532:quinone; D006861:Hydrogen Peroxide; D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase; D042845:Laccase; D010100:Oxygen