The use of self-interaction chromatography in stable formulation and crystallization of proteins.
This paper reviews the basic principles of the recently developed self-interaction chromatography (SIC) technique with regard to protein solution stability and protein crystallization. It gives experimental protocols for both normal-scale and micro-scale SIC experiments and reviews recent developments and current applications of this novel technique in the biopharmaceutical area. This paper aims to be a benchmark in the further proliferation of this highly effective and fast technology for the rational design of stable aqueous formulations of therapeutic proteins and the determination of solution conditions favoring protein crystallization.
Deshpande
Kedar S
KS
Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Ahamed
Tangir
T
ter Horst
Joop H
JH
Jansens
Peter J
PJ
van der Wielen
Luuk A M
LA
Ottens
Marcel
M
D002845:Chromatography / Q000379:methods*; D003460:Crystallization / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*
D011506:Proteins