A novel glucose biosensor based on the immobilization of glucose oxidase onto gold nanoparticles-modified Pb nanowires.
A novel glucose biosensor was developed, based on the immobilization of glucose oxidase (GOD) with cross-linking in the matrix of bovine serum albumin (BSA) on a Pt electrode, which was modified with gold nanoparticles decorated Pb nanowires (GNPs-Pb NWs). Pb nanowires (Pb NWs) were synthesized by an L-cysteine-assisted self-assembly route, and then gold nanoparticles (GNPs) were attached onto the nanowire surface through -SH-Au specific interaction. The morphological characterization of GNPs-Pb NWs was examined by transmission electron microscopy (TEM). Cyclic voltammetry and chronoamperometry were used to study and to optimize the electrochemical performance of the resulting biosensor. The synergistic effect of Pb NWs and GNPs made the biosensor exhibit excellent electrocatalytic activity and good response performance to glucose. The effects of pH and applied potential on the amperometric response of the biosensor have been systemically studied. In pH 7.0, the biosensor showed the sensitivity of 135.5 microA mM(-1) cm(-2), the detection limit of 2 microM (S/N=3), and the response time <5 s with a linear range of 5-2200 microM. Furthermore, the biosensor exhibits good reproducibility, long-term stability and relative good anti-interference.
Wang
Huicai
H
The Key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Wang
Xinsheng
X
Zhang
Xiaoqing
X
Qin
Xia
X
Zhao
Zixia
Z
Miao
Zhiying
Z
Huang
Nan
N
Chen
Qiang
Q
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D012710:Serum Albumin, Bovine; D007854:Lead; D010984:Platinum; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose