title: [Nav1.8 and Nav1.9 mRNA expression in rat trigeminal ganglion at different interval after molar extraction].
abstract: OBJECTIVE
  To observe the expression and function of extraction.
  
  
  METHODS
  Real-time reverse transcription PCR paralleled with vitro-established cRNA standard curves was applied to measure the expression of Nav1.8, Nav1.9 at 30 min, 2 h, 1 d, 3 d and 6 d respectively after extraction of rat right mandibular molars. The right mandibular molars were used as control.
  
  
  RESULTS
  Both Nav1.8 and Nav1.9 mRNA in right trigeminal ganglion showed little change after 30 min, and increased slowly after 2 h. Nav1.8, Nav1.9 mRNA expressions increased by 27% and 24.5% respectively compared to the left trigeminal ganglion after 3 d, reaching the highest level (P < 0.05), and then the expressions began decreasing from 6 d.
  
  
  CONCLUSIONS
  The pain caused by molar extraction is related to the up-regulation of expressions of sodium channels protein Nav1.8 and Nav1.9 mRNA, indicating the participation of sodium channels in regulations of peripheral tissue pain after molar extraction.
authors: Stomatological Center, General Hospital of PLA, Beijing 100853, China.
authors: Lei
authors: L
authors: Zhang
authors: Hong-Chen
authors: HC
authors: Liu
authors: Dong-Sheng
authors: DS
authors: Wang
mesh_terms: D000818:Animals; D008297:Male; D062559:NAV1.8 Voltage-Gated Sodium Channel; D062558:NAV1.9 Voltage-Gated Sodium Channel; D009479:Neuropeptides / Q000378:metabolism*; D010149:Postoperative Pain / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000378:metabolism*; D014081:Tooth Extraction; D012668:Trigeminal Ganglion / Q000378:metabolism*
chemical_list: D062559:NAV1.8 Voltage-Gated Sodium Channel; D062558:NAV1.9 Voltage-Gated Sodium Channel; D009479:Neuropeptides; D012333:RNA, Messenger; C568319:Scn10a protein, rat; C113705:Scn11a protein, rat; D015222:Sodium Channels