Serum trypsin inhibitory capacity in hemodialysis patients.
It has been established that overproduction of reactive oxygen species (ROS) occurs during hemodialysis causing oxidation of proteins. Alpha-1-antitrypsin is the major circulating anti-protease which contains methionine in the active site. The aim of the present study was to measure the level of serum trypsin inhibitory capacity (sTIC) in hemodialysis patients. This case-control study was performed in 52 hemodialysis patients and 49 healthy controls. sTIC was measured by enzymatic assay. The sTIC was significantly (P< 0.001) lower in hemodialysis patients (1.87 +/- 0.67 micromol/min/mL) than healthy controls (2.83 +/- 0.44 micromol/min/L). Reduction of sTIC may be due to the oxidation of methionine residue in the reactive site of alpha-1 antitrypsin.
Hashemi
Mohammad
M
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. hashemim@zdmu.ac.ir
Mehrabifar
Hamid
H
Homayooni
Fatemeh
F
Naderi
Mohammad
M
Montazerifar
Farzaneh
F
Ghavami
Saeid
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D008297:Male; D008715:Methionine / Q000378:metabolism; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D006435:Renal Dialysis; D055815:Young Adult; D000515:alpha 1-Antitrypsin / Q000097:blood*
D000515:alpha 1-Antitrypsin; D008715:Methionine