Wound-healing activity of a proteolytic fraction from Carica candamarcensis on experimentally induced burn.
Carica candamarcensis is a species from the Caricaceae family whose immature fruit contains latex with large amounts of cysteine proteinases. In prior studies, we isolated two of these enzymes displaying mitogenic activity when incubated with L929 fibroblastic cells. One of the fractions containing these enzymes (P1G10) was shown to enhance wound healing of skin and to accelerate healing of chemically induced gastric ulcer. In this study we evaluate the effect of P1G10 on heat-induced, third-degree burn using a rodent model. The results show that 0.1% P1G10 accelerates epithelisation while the effect of 1% or 0.01% P1G10 is not significantly different to 1% silver sulphadiazine, 2% papain or the hydrosoluble vehicle used as control. In a double-blind randomised experiment comparing the healing response of 0.1%, 1% and the vehicle alone, we confirmed the enhanced healing property of P1G10. Histological analysis of burn-tissue sections following treatment with P1G10 support these observations. These results extend the healing properties of these groups of enzymes to a different type of trauma and open the way to future clinical applications.
Gomes
Flávia S L
FS
Departamentos de Bioquímica e Imunologia, Farmacologia, Patologia Geral, Instituto de Ciências Biológicas, Escola de Enfermagem, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627, Belo Horizonte, MG, Brazil.
Spínola
Cássia de V
Cde V
Ribeiro
Henrique A
HA
Lopes
Miriam T P
MT
Cassali
Geovanni D
GD
Salas
Carlos E
CE
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D029441:Carica / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use; D011897:Random Allocation; D014945:Wound Healing / Q000187:drug effects*
D010936:Plant Extracts