title: Development of a rapid and sensitive magnetic chemiluminescent enzyme immunoassay for detection of luteinizing hormone in human serum.
abstract: OBJECTIVE
  A rapid and sensitive magnetic particles (MPs)-based chemiluminescence enzyme immunoassay (MPs-CLEIA) for determination of luteinizing hormone (LH) in human serum was developed and validated.
  
  
  METHODS
  The method was developed based on a sandwiched immunoreaction format, in which MPs served as both the solid phase and separator. The horseradish peroxidase(HRP)-luminol-H(2)O(2) chemiluminescent system was chosen as the detection system.
  
  
  RESULTS
  This method had a detection limit of 0.2 mIU/mL and a linear range of 0.5-200 mIU/mL. The assay showed little cross-reactivity, and the intra- and inter-assay coefficients of variation were less than 10%. The recoveries were from 101.6% to 104.1%. The method has been successfully applied to detection of LH in human serum and showed good correlation compared with radioimmunoassay (RIA).
  
  
  CONCLUSIONS
  The MPs-CLEIA provided apparent advantages over RIA, and facilitated the development of high-throughput screening and automated operation systems in clinical diagnosis.
authors: The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors: Qin
authors: Q
authors: Xiao
authors: Haifang
authors: H
authors: Li
authors: Guomao
authors: G
authors: Hu
authors: Hongrui
authors: H
authors: Wang
authors: Zhenjia
authors: Z
authors: Li
authors: Jin-Ming
authors: JM
authors: Lin
mesh_terms: D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D049449:Luminescence; D008165:Luminol / Q000737:chemistry; D007986:Luteinizing Hormone / Q000097:blood* / Q000737:chemistry; D008280:Magnetics; D015203:Reproducibility of Results
chemical_list: D008165:Luminol; D007986:Luteinizing Hormone; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase