High expression of HSP47 in ulcerative colitis-associated carcinomas: proteomic approach.
BACKGROUND
Ulcerative colitis (UC) is a chronic relapsing inflammatory bowel disease, known to be associated with a markedly increased risk of colorectal carcinoma development.


METHODS
Using proteomic analysis with two-dimensional gel electrophoresis and mass spectrometry, differentially expressed proteins were assessed between UC-associated cancer and sporadic colon cancer cell lines. Western blot and immunostaining were performed for confirming the expression.


RESULTS
Heat-shock protein of 47 kDa (HSP47) was identified as one of the proteins expressed more highly in UC-associated cancer cell lines, and an immunohistochemical examination confirmed significantly higher levels of HSP47 in UC-associated colon cancers than in sporadic counterparts, the expression increasing with a progression of neoplastic lesions. Heat-shock protein of 47 kDa was further found to be coexpressed with type I collagen in the cytoplasm, and both HSP47 and type I collagen were released from cultured cells into the culture medium.


CONCLUSIONS
These results suggest that overexpression of HSP47 is a unique characteristic of UC-associated carcinoma related to type I collagen synthesis, with possible clinical applications.
Araki
K
K
Department of Cellular and Histo-pathology, Kitasato University Postgraduate School of Medical Sciences, Sagamihara, Kanagawa, Japan. dm07003o@st.kitasato-u.ac.jp
Mikami
T
T
Yoshida
T
T
Kikuchi
M
M
Sato
Y
Y
Oh-ishi
M
M
Kodera
Y
Y
Maeda
T
T
Okayasu
I
I
D015153:Blotting, Western; D045744:Cell Line, Tumor; D003093:Colitis, Ulcerative / Q000150:complications*; D024042:Collagen Type I / Q000032:analysis; D015179:Colorectal Neoplasms / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D050958:HSP47 Heat-Shock Proteins / Q000032:analysis* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D040901:Proteomics
D024042:Collagen Type I; D050958:HSP47 Heat-Shock Proteins; C494660:SERPINH1 protein, human