Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself.
EMT-6 mammary carcinoma and B16 melanoma (B16M) cells are lethal and barely immunogenic in syngeneic BALB/c and C57BL/6 mice, respectively. We show that mice vaccinated with tumor cells pulsed with a MHC class I-restricted peptide develop a T cell response, not only to the peptide, but also to the unpulsed tumor. These mice display protective immunity against the unpulsed tumor, and their T cells adoptively transfer tumor-specific protection to immunodeficient SCID mice. Our data have implications for cancer vaccine strategies. Grafting a single well-defined foreign peptide on tumor cells might suffice to trigger anti-tumor immunity.
Schlingmann
Tobias R
TR
Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.
Rininsland
Frauke H
FH
Bartholomae
Wolf C
WC
Kuekrek
Haydar
H
Lehmann
Paul V
PV
Tary-Lehmann
Magdalena
M
D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D015674:Mammary Neoplasms, Animal / Q000276:immunology* / Q000628:therapy; D008546:Melanoma, Experimental / Q000276:immunology* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination
D019496:Cancer Vaccines; D015395:Histocompatibility Antigens Class I; D010455:Peptides