title: The protective immune response against infectious bronchitis virus induced by multi-epitope based peptide vaccines.
abstract: Peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. To explore multi-epitope based peptide vaccines against infectious bronchitis virus (IBV), the immunogenic peptides were fused to the 3' terminal of glutathione S transferase gene (GST) and expressed in Escherichia coli. ELISA and Western blot analysis showed that the purified fusion proteins had excellent immune activity with chicken anti-IBV serum. During the vaccination course, the candidate peptide vaccines induced strong humoral and cellular response, and provided up to 80.0% immune protection, while all non-immunized chickens in the negative control group manifested obvious typical symptoms and died after virus challenge. Our finding provides a new way to develop multi-epitope based peptide vaccine against IBV.
authors: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, School of Life Sciences, Sichuan University, Chengdu, PR China.
authors: Tai
authors: T
authors: Yang
authors: Hong-Ning
authors: HN
authors: Wang
authors: Xue
authors: X
authors: Wang
authors: Jun-Ni
authors: JN
authors: Tang
authors: Dan
authors: D
authors: Lu
authors: Yun-Fei
authors: YF
authors: Zhang
authors: Zi-Cheng
authors: ZC
authors: Guo
authors: Yu-Ling
authors: YL
authors: Li
authors: Rong
authors: R
authors: Gao
authors: Run-Min
authors: RM
authors: Kang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D049109:Cell Proliferation; D002645:Chickens / Q000276:immunology / Q000821:virology; D018352:Coronavirus Infections / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D056724:Immunity, Humoral / Q000276:immunology; D001351:Infectious bronchitis virus / Q000276:immunology* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology*
chemical_list: D000906:Antibodies; D000956:Antigens, Viral; D000939:Epitopes; D022223:Vaccines, Subunit