title: Genotoxicity and antigenotoxicity evaluation of non-photoactivated hypericin.
abstract: The potential genotoxicity and antigenotoxicity of non-photoactivated hypericin was investigated in five experimental models. Hypericin was non-mutagenic in the Ames assay, with and without metabolic activation. It did not exert a protective effect against mutagenicity induced by 9-aminoacridine. In a yeast (Saccharomyces cerevisiae) assay, hypericin did not increase the frequency of mitotic crossovers or total aberrants at the ade(2) locus, the number of convertants at the trp5 locus, or the number of revertants at the ilv1 locus. In combined application with 4-nitroquinoline-1-oxide, it significantly enhanced the number of revertants at the ilv1 locus at the highest concentration used. Hypericin was not mutagenic in the alga Chlamydomonas reinhardtii. However, in combined application with methyl methane sulfonate, toxicity and mutagenicity were slightly reduced. In a chromosome aberration assay using three mammalian cell lines, hypericin did not alter the frequency of structural chromosome aberrations, and in the DPPH radical scavenging assay, it did not exert any antioxidant effects.
authors: Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynska dolina B1, 842 15 Bratislava, Slovakia.
authors: Eva
authors: E
authors: Miadokova
authors: Ivan
authors: I
authors: Chalupa
authors: Viera
authors: V
authors: Vlckova
authors: Andrea
authors: A
authors: Sevcovicova
authors: Slavomira
authors: S
authors: Nadova
authors: Marcela
authors: M
authors: Kopaskova
authors: Alena
authors: A
authors: Hercegova
authors: Patricia
authors: P
authors: Gasperova
authors: Lubica
authors: L
authors: Alfoldiova
authors: Monika
authors: M
authors: Komjatiova
authors: Zuzana
authors: Z
authors: Csanyiova
authors: Eliska
authors: E
authors: Galova
authors: Eva
authors: E
authors: Cellarova
authors: Daniel
authors: D
authors: Vlcek
mesh_terms: D000818:Animals; D000873:Anthracenes; D016587:Antimutagenic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016825:Chlamydomonas reinhardtii / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced; D006224:Cricetinae; D004249:DNA Damage; D006801:Humans; D009152:Mutagenicity Tests; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects
chemical_list: D000873:Anthracenes; D016587:Antimutagenic Agents; D010569:Perylene; C004965:hypericin