[Study on radiation sensitivity of colorectal cancer cells using gene expression profile].
OBJECTIVE
To screen the radiosensitivity-related genes of colorectal cancer cells.


METHODS
Gene expression profiles of two different radiosensitivity cells(colorectal cancer cell line Lovo and SW480) were obtained by cDNA array and the differences of gene expression profiles between the two cells were analyzed.


RESULTS
Genes of more than 2-fold expressive differentiation were screened. In Lovo cells, 908 up-regulated genes were found, including higher expression genes CEACAM5, THBS1, SERPINE2, ARL7, HPGD, while 1312 genes were down-regulated. In SW480 cells, higher expression genes were SCD, NQ01, LYZ, KRT20 and ATP1B1.


CONCLUSIONS
Gene profiles can reflect the radiosensitivity of colorectal cancer cells, which will provide the choice for the further study of radiosensitivity in colorectal cancer.
Yang
Xiao-dong
XD
Department of General Surgery, The Second Affiliated Hospital, Suzhou University, Suzhou 215004, China.
Xing
Chun-gen
CG
Gong
Wei
W
Zhou
Li-ying
LY
Wu
Yong-you
YY
Zhao
Kui
K
D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011836:Radiation Tolerance / Q000235:genetics*