Acyl enzyme intermediates in sortase-catalyzed pilus morphogenesis in gram-positive bacteria.
In gram-positive bacteria, covalently linked pilus polymers are assembled by a specific transpeptidase enzyme called pilus-specific sortase. This sortase is postulated to cleave the LPXTG motif of a pilin precursor between threonine and glycine and to form an acyl enzyme intermediate with the substrate. Pilus polymerization is believed to occur through the resolution of this intermediate upon specific nucleophilic attack by the conserved lysine located within the pilin motif of another pilin monomer, which joins two pilins with an isopeptide bond formed between threonine and lysine. Here, we present evidence for sortase reaction intermediates in Corynebacterium diphtheriae. We show that truncated SrtA mutants that are loosely bound to the cytoplasmic membrane form high-molecular-weight complexes with SpaA polymers secreted into the extracellular milieu. These complexes are not formed with SpaA pilin mutants that have alanine substitutions in place of threonine in the LPXTG motif or lysine in the pilin motif. The same phenotype is observed with alanine substitutions of either the conserved cysteine or histidine residue of SrtA known to be required for catalysis. Remarkably, the assembly of SpaA pili, or the formation of intermediates, is abolished with a SrtA mutant missing the membrane-anchoring domain. We infer that pilus polymerization involves the formation of covalent pilin-sortase intermediates, which occurs within a molecular platform on the exoplasmic face of the cytoplasmic membrane that brings together both sortase and its cognate substrates in close proximity to each other, likely surrounding a secretion apparatus. We present electron microscopic data in support of this picture.
Guttilla
Irene K
IK
Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030, USA.
Gaspar
Andrew H
AH
Swierczynski
Arlene
A
Swaminathan
Anu
A
Dwivedi
Prabhat
P
Das
Asis
A
Ton-That
Hung
H
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003353:Corynebacterium diphtheriae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D003470:Culture Media; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism* / Q000648:ultrastructure; D015964:Gene Expression Regulation, Bacterial; D006094:Gram-Positive Bacteria / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D009154:Mutation; D000069816:SEC Translocation Channels; D000081416:SecA Proteins
D001426:Bacterial Proteins; D003470:Culture Media; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins