Methods for energizing cell-free protein synthesis.
The productivity of cell-free protein synthesis depends critically upon the efficiency of ATP regeneration. In addition, the expensive energy sources used in the conventional cell-free protein synthesis systems account for the major part of the overall cost for the expression of proteins. Therefore, it is essential to implement a cheaper and more efficient ATP regeneration method in order to make cell-free protein synthesis a viable option for industrial protein production. In this article, we review the recent progress in the methodologies for supplying ATP during cell-free protein synthesis in E. coli extracts.
Kim
Ho-Cheol
HC
Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea.
Kim
Dong-Myung
DM
D002474:Cell-Free System; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D008274:Magnesium / Q000378:metabolism; D014176:Protein Biosynthesis; D019289:Pyruvic Acid / Q000378:metabolism
D003470:Culture Media; D019289:Pyruvic Acid; D008274:Magnesium; D005947:Glucose