title: Elevated concentrations of sphingosylphosphorylcholine in cerebrospinal fluid after subarachnoid hemorrhage: a possible role as a spasmogen.
abstract: This study investigates the role of sphingosylphosphorylcholine (SPC) in the mechanisms underlying cerebral vasospasm after subarachnoid hemorrhage (SAH). The levels of SPC were measured in cerebrospinal fluid (CSF) of patients with SAH and also in an experimental canine model. CSF samples were collected from 11 patients with SAH, and from dogs that had received an injection of SPC into the cisterna magna to examine SPC kinetics in the CSF. SPC was assayed using solid-phase extraction and triple quadrupole mass spectrometry. The SPC concentrations in SAH patients on days 3, 8, and 14 after the onset of SAH were significantly higher than those in normal CSF. In the canine model, rapid dilution of SPC in CSF was observed. In combination with data from previous studies, these results suggest that SPC is involved in the development of cerebral vasospasm. Rapid dilution of SPC in CSF suggests that SPC is released into CSF at higher concentrations than those measured in the present study.
authors: Department of Neurosurgery, Yamaguchi University School of Medicine, Minamikogushi, Ube, Yamaguchi, Japan.
authors: Tetsu
authors: T
authors: Kurokawa
authors: Yasunobu
authors: Y
authors: Yumiya
authors: Hirosuke
authors: H
authors: Fujisawa
authors: Satoshi
authors: S
authors: Shirao
authors: Shiro
authors: S
authors: Kashiwagi
authors: Masafumi
authors: M
authors: Sato
authors: Hiroko
authors: H
authors: Kishi
authors: Saori
authors: S
authors: Miwa
authors: Kimiko
authors: K
authors: Mogami
authors: Shoichi
authors: S
authors: Kato
authors: Tatsuo
authors: T
authors: Akimura
authors: Masaaki
authors: M
authors: Soma
authors: Kuniaki
authors: K
authors: Ogasawara
authors: Akira
authors: A
authors: Ogawa
authors: Sei
authors: S
authors: Kobayashi
authors: Michiyasu
authors: M
authors: Suzuki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid / Q000737:chemistry; D012044:Regression Analysis; D052616:Solid Phase Extraction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid / Q000737:chemistry; D013345:Subarachnoid Hemorrhage / Q000134:cerebrospinal fluid*
chemical_list: C005356:sphingosine phosphorylcholine; D010767:Phosphorylcholine; D013110:Sphingosine