Preliminary crystallographic studies of the regulatory domain of response regulator YycF from an essential two-component signal transduction system.
YycGF is a crucial signal transduction system for the regulation of cell-wall metabolism in low-G+C Gram-positive bacteria, which include many important human pathogens. The response regulator YycF receives signals from its cognate histidine kinase YycG through a phosphotransfer reaction and elicits responses through regulation of gene expression. The N-terminal regulatory domain of YycF from Bacillus subtilis was overproduced and purified. The protein was crystallized and X-ray data were collected to 1.95 A resolution with a completeness of 97.7% and an overall R(merge) of 7.7%. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 59.50, c = 79.06 A.
Zhao
Haiyan
H
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA.
Heroux
Annie
A
Sequeira
Reuben D
RD
Tang
Liang
L
D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
D001426:Bacterial Proteins; C119939:YycF protein, Bacteria