Antiviral efficacy against hepatitis B virus replication of oleuropein isolated from Jasminum officinale L. var. grandiflorum.
BACKGROUND
Jasminum officinale L. var. grandiflorum (JOG) is a folk medicine used for the treatment of hepatitis in south of China. Phytochemical studies showed that secoiridoid glycosides are the typical constituents of this plant.


OBJECTIVE
The present study was undertaken to evaluate the effect of oleuropein (Ole) derived from the flowers of JOG on hepatitis B virus (HBV) replication in HepG2 2.2.15 cell line in vitro and duck hepatitis B virus (DHBV) replication in ducklings in vivo.


METHODS
The extracellular hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) concentrations in cell culture medium were determined by ELISA. DHBV in duck serum was analyzed by dot blot.


RESULTS
Ole blocks effectively HBsAg secretion in HepG2 2.2.15 cells in a dose-dependent manner (IC(50)=23.2 microg/ml). Ole (80 mg/kg, intraperitoneally, twice daily) also reduced viremia in DHBV-infected ducks.


CONCLUSIONS
Ole therefore warrants further investigation as a potential therapeutic agent for HBV infection.
Zhao
Guiqin
G
Institute of Radiation Medicine, Academy of Military Medical Science, Haidian District, Beijing 100850, China.
Yin
Zhifeng
Z
Dong
Junxing
J
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002460:Cell Line; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D035264:Flowers; D006509:Hepatitis B / Q000188:drug therapy / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D015611:Hepatitis B Virus, Duck / Q000187:drug effects / Q000502:physiology; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D006524:Hepatitis, Viral, Animal / Q000097:blood / Q000188:drug therapy* / Q000821:virology; D006801:Humans; D057888:Iridoid Glucosides; D039823:Iridoids; D031660:Jasminum / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011714:Pyrans / Q000494:pharmacology* / Q000627:therapeutic use; D014766:Viremia / Q000097:blood / Q000188:drug therapy; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; D011714:Pyrans; C002769:oleuropein