title: Transduced human PEP-1-catalase fusion protein attenuates ischemic neuronal damage.
abstract: Antioxidant enzymes are considered to have beneficial effects against various diseases mediated by reactive oxygen species (ROS). Ischemia is characterized by both oxidative stress and changes in the antioxidant defense system. Catalase (CAT) and superoxide dismutase (SOD) are major antioxidant enzymes by which cells counteract the deleterious effects of ROS. To investigate the protective effects of CAT, we constructed PEP-1-CAT cell-permeative expression vectors. When PEP-1-CAT fusion proteins were added to the culture medium of neuronal cells, they rapidly entered the cells and protected them against oxidative stress-induced neuronal cell death. Immunohistochemical analysis revealed that PEP-1-CAT prevented neuronal cell death in the hippocampus induced by transient forebrain ischemia. Moreover, we showed that the protective effect of PEP-1-CAT was observed in neuronal cells treated with PEP-1-SOD. Therefore, we suggest that transduced PEP-1-CAT and PEP-1-SOD fusion proteins could be useful as therapeutic agents for various human diseases related to oxidative stress, including stroke.
authors: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, College of Medicine, Hallym University, Chunchon 200-702, Korea.
authors: Dae Won
authors: DW
authors: Kim
authors: Hoon Jae
authors: HJ
authors: Jeong
authors: Hye Won
authors: HW
authors: Kang
authors: Min Jea
authors: MJ
authors: Shin
authors: Eun Jeong
authors: EJ
authors: Sohn
authors: Mi Jin
authors: MJ
authors: Kim
authors: Eun Hee
authors: EH
authors: Ahn
authors: Jae Jin
authors: JJ
authors: An
authors: Sang Ho
authors: SH
authors: Jang
authors: Ki-Yeon
authors: KY
authors: Yoo
authors: Moo Ho
authors: MH
authors: Won
authors: Tae-Cheon
authors: TC
authors: Kang
authors: In Koo
authors: IK
authors: Hwang
authors: Oh-Shin
authors: OS
authors: Kwon
authors: Sung-Woo
authors: SW
authors: Cho
authors: Jinseu
authors: J
authors: Park
authors: Won Sik
authors: WS
authors: Eum
authors: Soo Young
authors: SY
authors: Choi
mesh_terms: D002374:Catalase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D009474:Neurons / Q000378:metabolism* / Q000473:pathology*; D018384:Oxidative Stress; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C503924:Pep-1 peptide; D010455:Peptides; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D003543:Cysteamine; D002374:Catalase; C558128:PEP-1-catalase fusion protein