title: Characterization of cluster-assembled nanostructured titanium oxide coatings as substrates for protein arrays.
abstract: Protein microarray technologies are rapidly expanding to fulfill current needs of proteome discovery for disease management. Nanostructured materials have been shown to present interesting features when used in biological settings: nanostructured titanium oxide film (ns-TiOx), synthesized by supersonic cluster beam deposition (SCBD), has recently emerged as a biocompatible substrate in different biological assays. The ns-TiOx surface is characterized by a morphology at the nanoscale that can be tuned to modulate specific biomolecule-material interactions. Here we present a systematic characterization of ns-TiOx coatings as protein binding surfaces, comparing their performances with those of most common commercial substrates in protein and antibody microarray assays. Through a robust statistical evaluation of repeatability in terms of coefficient of variation (CV) analysis, we demonstrate that ns-TiOx can be used as reliable substrate for biochips in analytical protein microarray application.
authors: Tethis, Via Russoli 3, 20143 Milano, Italy. roberta.carbone@tethis-lab.com
authors: Roberta
authors: R
authors: Carbone
authors: Marzia
authors: M
authors: De Marni
authors: Andrea
authors: A
authors: Zanardi
authors: Simone
authors: S
authors: Vinati
authors: Emanuele
authors: E
authors: Barborini
authors: Lorenzo
authors: L
authors: Fornasari
authors: Paolo
authors: P
authors: Milani
mesh_terms: D000327:Adsorption; D000704:Analysis of Variance; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000737:chemistry; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D014025:Titanium / Q000737:chemistry*
chemical_list: D000906:Antibodies; D011506:Proteins; C009495:titanium dioxide; D014025:Titanium