title: Gene and pathway-based second-wave analysis of genome-wide association studies.
abstract: Despite the great success of genome-wide association studies (GWAS) in identification of the common genetic variants associated with complex diseases, the current GWAS have focused on single-SNP analysis. However, single-SNP analysis often identifies only a few of the most significant SNPs that account for a small proportion of the genetic variants and offers only a limited understanding of complex diseases. To overcome these limitations, we propose gene and pathway-based association analysis as a new paradigm for GWAS. As a proof of concept, we performed a comprehensive gene and pathway-based association analysis of 13 published GWAS. Our results showed that the proposed new paradigm for GWAS not only identified the genes that include significant SNPs found by single-SNP analysis, but also detected new genes in which each single SNP conferred a small disease risk; however, their joint actions were implicated in the development of diseases. The results also showed that the new paradigm for GWAS was able to identify biologically meaningful pathways associated with the diseases, which were confirmed by a gene-set-rich analysis using gene expression data.
authors: School of Life Science, Fudan University, Shanghai, China.
authors: Gang
authors: G
authors: Peng
authors: Li
authors: L
authors: Luo
authors: Hoicheong
authors: H
authors: Siu
authors: Yun
authors: Y
authors: Zhu
authors: Pengfei
authors: P
authors: Hu
authors: Shengjun
authors: S
authors: Hong
authors: Jinying
authors: J
authors: Zhao
authors: Xiaodong
authors: X
authors: Zhou
authors: John D
authors: JD
authors: Reveille
authors: Li
authors: L
authors: Jin
authors: Christopher I
authors: CI
authors: Amos
authors: Momiao
authors: M
authors: Xiong
mesh_terms: D001172:Arthritis, Rheumatoid / Q000235:genetics; D053263:Gene Regulatory Networks / Q000235:genetics*; D055106:Genome-Wide Association Study; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results
chemical_list: D007987:Gonadotropin-Releasing Hormone