Quantitative analysis of mitragynine in human urine by high performance liquid chromatography-tandem mass spectrometry.
Mitragynine is the primary active alkaloid extracted from the leaves of Mitragyna speciosa Korth, a plant that originates in South-East Asia and is commonly known as kratom in Thailand. Kratom has been used for many centuries for their medicinal and psychoactive qualities, which are comparable to that of opiate-based drugs. Kratom abuse can lead to a detectable content of mitragynine residue in urine. Ultra trace amount of mitragynine in human urine was determined by a high performance liquid chromatography coupled to an electrospray tandem mass spectrometry (HPLC-ESI/MS/MS). Mitragynine was extracted by methyl t-butyl ether (MTBE) and separated on a HILIC column. The ESI/MS/MS was accomplished using a triple quadrupole mass spectrometer in positive ion detection and multiple reactions monitoring (MRM) mode. Ajmalicine, a mitragynine's structure analog was selected as internal standard (IS) for method development. Quality control (QC) performed at three levels 0.1, 1 and 5 ng/ml of mitragynine in urine gave mean recoveries of 90, 109, and 98% with average relative standard deviation of 22, 12 and 16%, respectively. The regression linearity of mitragynine calibration ranged from 0.01 to 5.0 ng/ml was achieved with correlation coefficient greater than 0.995. A detection limit of 0.02 ng/ml and high precision data within-day and between days analysis were obtained.
Lu
Shijun
S
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, NY 12201-0509, USA. lus@wadsworth.org
Tran
Buu N
BN
Nelsen
Jamie L
JL
Aldous
Kenneth M
KM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000652:urine*; D046948:Secologanin Tryptamine Alkaloids / Q000652:urine*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013287:Illicit Drugs; D046948:Secologanin Tryptamine Alkaloids; C001801:mitragynine