Detection and quantification of neurotransmitters in dialysates.
Sensitive analytical methods are needed for the separation and quantification of neurotransmitters obtained in microdialysate studies. This unit describes methods that permit quantification of nanomolar concentrations of monoamines and their metabolites (high-pressure liquid chromatography electrochemical detection), acetylcholine (HPLC-coupled to an enzyme reactor), and amino acids (HPLC-fluorescence detection; capillary electrophoresis with laser-induced fluorescence detection).
Zapata
Agustin
A
NIH, NIDA Intramural Research Program, Baltimore, Maryland, USA.
Chefer
Vladimir I
VI
Shippenberg
Toni S
TS
Denoroy
Luc
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D006801:Humans; D018377:Neurotransmitter Agents / Q000032:analysis*
D018377:Neurotransmitter Agents