title: Transcriptional profiles of adult male and female Schistosoma japonicum in response to insulin reveal increased expression of genes involved in growth and development.
abstract: Microarray analysis was used to investigate differential gene regulation in adult male and female Schistosoma japonicum cultured in the presence or absence of insulin in vitro. A total of 1,101 genes were up- or down-regulated in response to insulin, the majority of differential expression occurring 24h after the addition of insulin to the cultures. Genes differentially expressed in male or female worms were predominantly involved in growth and development, with significant sex-specific differences in transcriptional profiles evident. Insulin appeared to promote protein synthesis and control protein degradation more prominently in male parasites. The study also indicated that insulin plays a more pronounced role in the uptake of glucose in unpaired female parasites, as reflected in the increased stimulation of gene expression of the phosphatidylinositol 3-kinase sub-pathway of insulin signalling. Insulin may also impact on the sexual differentiation and fecundity of female schistosomes by activation of the mitogenic-activated protein kinase sub-pathway.
authors: Division of Infectious Diseases, Queensland Institute of Medical Research, Brisbane, Qld, Australia.
authors: Hong
authors: H
authors: You
authors: Wenbao
authors: W
authors: Zhang
authors: Luke
authors: L
authors: Moertel
authors: Donald P
authors: DP
authors: McManus
authors: Geoffrey N
authors: GN
authors: Gobert
mesh_terms: D000818:Animals; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D046228:Microarray Analysis; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D012737:Sex Factors; D015398:Signal Transduction
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin