Long-term cell culture of electrically active sensory neurons free of glial cells.
A method has been devised for long-term culturing of sensory ganglion cells, free of non-neuronal cells. After enzymatic-mechanical disaggregation of embryonic chick sensory ganglia the cell suspension is plated in dispersed cell culture. After 2-3 weeks in culture neurons and satellite cells re-associate in a histiotypic fashion. Partial or complete elimination of non-neuronal cells is achieved by treatment of the cultures with fluorodeoxyuridine. The glia-deprived neurons are electrophysiologically active and can be maintained in cell culture for more than 5 months.
Rieske
E
E
Max Planck Institute for Psychiatry, D-8000 Munich, Kraepelinstr. 2 G.F.R.
Kreutzberg
G W
GW