Acute cold exposure-induced down-regulation of CIDEA, cell death-inducing DNA fragmentation factor-alpha-like effector A, in rat interscapular brown adipose tissue by sympathetically activated beta3-adrenoreceptors.
The thermogenic activity of brown adipose tissue (BAT) largely depends on the mitochondrial uncoupling protein 1 (UCP1), which is up-regulated by environmental alterations such as cold. Recently, CIDEA (cell death-inducing DNA fragmentation factor-alpha-like effector A) has also been shown to be expressed at high levels in the mitochondria of BAT. Here we examined the effect of cold on the mRNA and protein levels of CIDEA in interscapular BAT of conscious rats with regard to the sympathetic nervous system. Cold exposure (4 degrees C for 3h) elevated the plasma norepinephrine level and increased norepinephrine turnover in BAT. Cold exposure resulted in down-regulation of the mRNA and protein levels of CIDEA in BAT, accompanied by up-regulation of mRNA and protein levels of UCP1. The cold exposure-induced changes of CIDEA and UCP1 were attenuated by intraperitoneal pretreatment with propranolol (a non-selective beta-adrenoreceptor antagonist) (2mg/animal) or SR59230A (a selective beta(3)-adrenoreceptor antagonist) (2mg/animal), respectively. These results suggest that acute cold exposure resulted in down-regulation of CIDEA in interscapular BAT by sympathetically activated beta(3)-adrenoreceptor-mediated mechanisms in rats.
Shimizu
Takahiro
T
Department of Pharmacology, School of Medicine, Kochi University, Nankoku, Kochi 783-8505, Japan. shimizu@kochi-u.ac.jp
Yokotani
Kunihiko
K
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D058673:Adrenergic beta-3 Receptor Antagonists; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D001833:Body Temperature Regulation; D003080:Cold Temperature; D015536:Down-Regulation; D007473:Ion Channels / Q000096:biosynthesis; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D009638:Norepinephrine / Q000097:blood / Q000378:metabolism; D011412:Propanolamines / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D000071256:Uncoupling Protein 1
C097869:3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate; D058673:Adrenergic beta-3 Receptor Antagonists; D000319:Adrenergic beta-Antagonists; D051017:Apoptosis Regulatory Proteins; C542951:CIDEA protein, rat; D007473:Ion Channels; D024101:Mitochondrial Proteins; D011412:Propanolamines; D022702:Receptors, Adrenergic, beta-3; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D011433:Propranolol; D009638:Norepinephrine