title: Integrated microfluidic electrochemical DNA sensor.
abstract: Effective systems for rapid, sequence-specific nucleic acid detection at the point of care would be valuable for a wide variety of applications, including clinical diagnostics, food safety, forensics, and environmental monitoring. Electrochemical detection offers many advantages as a basis for such platforms, including portability and ready integration with electronics. Toward this end, we report the Integrated Microfluidic Electrochemical DNA (IMED) sensor, which combines three key biochemical functionalities--symmetric PCR, enzymatic single-stranded DNA generation, and sequence-specific electrochemical detection--in a disposable, monolithic chip. Using this platform, we demonstrate detection of genomic DNA from Salmonella enterica serovar Typhimurium LT2 with a limit of detection of <10 aM, which is approximately 2 orders of magnitude lower than that from previously reported electrochemical chip-based methods.
authors: Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, USA.
authors: Brian S
authors: BS
authors: Ferguson
authors: Steven F
authors: SF
authors: Buchsbaum
authors: James S
authors: JS
authors: Swensen
authors: Kuangwen
authors: K
authors: Hsieh
authors: Xinhui
authors: X
authors: Lou
authors: H Tom
authors: HT
authors: Soh
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques; D020411:Oligonucleotide Array Sequence Analysis; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial