title: Metabolic network analysis integrated with transcript verification for sequenced genomes.
abstract: With sequencing of thousands of organisms completed or in progress, there is a growing need to integrate gene prediction with metabolic network analysis. Using Chlamydomonas reinhardtii as a model, we describe a systems-level methodology bridging metabolic network reconstruction with experimental verification of enzyme encoding open reading frames. Our quantitative and predictive metabolic model and its associated cloned open reading frames provide useful resources for metabolic engineering.
authors: Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
authors: Ani
authors: A
authors: Manichaikul
authors: Lila
authors: L
authors: Ghamsari
authors: Erik F Y
authors: EF
authors: Hom
authors: Chenwei
authors: C
authors: Lin
authors: Ryan R
authors: RR
authors: Murray
authors: Roger L
authors: RL
authors: Chang
authors: S
authors: S
authors: Balaji
authors: Tong
authors: T
authors: Hao
authors: Yun
authors: Y
authors: Shen
authors: Arvind K
authors: AK
authors: Chavali
authors: Ines
authors: I
authors: Thiele
authors: Xinping
authors: X
authors: Yang
authors: Changyu
authors: C
authors: Fan
authors: Elizabeth
authors: E
authors: Mello
authors: David E
authors: DE
authors: Hill
authors: Marc
authors: M
authors: Vidal
authors: Kourosh
authors: K
authors: Salehi-Ashtiani
authors: Jason A
authors: JA
authors: Papin
mesh_terms: D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering; D018503:Genome, Protozoan; D008957:Models, Genetic; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004798:Enzymes; D015800:Protozoan Proteins