title: [Mutation analysis of neural cell adhesion molecules in human astrocytoma].
abstract: OBJECTIVE
  To identify the function of neural cell adhesion molecule (NCAM) mutation in the genesis of human glioma.
  
  
  METHODS
  Mutations were found through polymerase chain reaction-single strand conformation polymorphism, and then the changed DNA fragments were purified and multiplied and sent to Shanghai for sequencing. Blast and ORF finder were used to find out the amino changes in NCAM.
  
  
  RESULTS
  An A-C transversion was found at position 1 126 in NCAM's 7 exon in a patient with glioblastoma from 43 astrocytoma.
  
  
  CONCLUSIONS
  Structural change in the protein caused by point mutation may be the reason for tumorigenesis of astrocytoma.
authors: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 400008, China.
authors: Yanjin
authors: Y
authors: Wang
authors: Juanjuan
authors: J
authors: Xiang
authors: Jiasheng
authors: J
authors: Fang
mesh_terms: D000328:Adult; D001254:Astrocytoma / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D019002:CD56 Antigen / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation
chemical_list: D019002:CD56 Antigen; C522918:NCAM1 protein, human