An immune responsive complement factor D/adipsin and kallikrein-like serine protease (PoDAK) from the olive flounder Paralichthys olivaceus.
The cDNA encoding of a complement factor D/adipsin and kallikrein-like serine protease, designated PoDAK, was isolated from the olive flounder Paralichthys olivaceus. PoDAK cDNA encodes a polypeptide with 277 amino acids containing conserved catalytic triad residues of serine proteases. The amino acid sequence of PoDAK showed high similarity to the kallikrein-like protein of medaka, mammalian adipsin/complement factor D and tissue kallikrein homolog, KT-14 of trout, complement factor D of zebrafish, and shared 31.6-36.8% homology with complement factor D/adipsin known from other species, including mammals. Phylogenetic analysis revealed that PoDAK clustered with the kallikrein-like protein of medaka and mammalian adipsin/complement factor D and tissue kallikrein homolog KT-14 of trout. The expression of PoDAK mRNA was high in the gills and heart, moderate in muscle, liver, intestine, stomach, kidney, and spleen of healthy flounder, and increased in the kidney, liver, and spleen of flounder challenged by the viral hemorrhagic septicemia virus (VHSV) or Streptococcus iniae. In situ hybridization confirmed that PoDAK mRNA is localized in the kidney and heart of individuals infected with VHSV. Further investigations are needed to clarify the function of PoDAK in vivo and in vitro.
Kong
Hee Jeong
HJ
Biotechnology Research Division, National Fisheries Research and Development Institute, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan 619-705, Republic of Korea. konghj@nfrdi.go.kr
Hong
Gyeong-Eun
GE
Nam
Bo-Hye
BH
Kim
Young-Ok
YO
Kim
Woo-Jin
WJ
Lee
Sang-Jun
SJ
Lee
Nam-Sil
NS
Do
Jeong Wan
JW
Cho
Hyun Kook
HK
Cheong
Jaehun
J
Lee
Chang Hoon
CH
Kim
Kyung-Kil
KK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D011416:Complement Factor D / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D005393:Fish Diseases / Q000201:enzymology / Q000276:immunology; D005432:Flounder / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007610:Kallikreins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D029141:Novirhabdovirus; D010802:Phylogeny; D012333:RNA, Messenger / Q000276:immunology; D018353:Rhabdoviridae Infections / Q000201:enzymology / Q000276:immunology / Q000662:veterinary; D016415:Sequence Alignment
D012333:RNA, Messenger; D007610:Kallikreins; D011416:Complement Factor D