title: Gaussia luciferase variant for high-throughput functional screening applications.
abstract: Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.
authors: Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston Massachusetts 02115, USA.
authors: Casey A
authors: CA
authors: Maguire
authors: Nikolaos C
authors: NC
authors: Deliolanis
authors: Lisa
authors: L
authors: Pike
authors: Johanna M
authors: JM
authors: Niers
authors: Lee-Ann
authors: LA
authors: Tjon-Kon-Fat
authors: Miguel
authors: M
authors: Sena-Esteves
authors: Bakhos A
authors: BA
authors: Tannous
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D033342:Copepoda / Q000201:enzymology*; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D008156:Luciferases