Pyrin Modulates the Intracellular Distribution of PSTPIP1.
PSTPIP1 is a cytoskeleton-associated adaptor protein that links PEST-type phosphatases to their substrates. Mutations in PSTPIP1 cause PAPA syndrome (Pyogenic sterile Arthritis, Pyoderma gangrenosum, and Acne), an autoinflammatory disease. PSTPIP1 binds to pyrin and mutations in pyrin result in familial Mediterranean fever (FMF), a related autoinflammatory disorder. Since disease-associated mutations in PSTPIP1 enhance pyrin binding, PAPA syndrome and FMF are thought to share a common pathoetiology. The studies outlined here describe several new aspects of PSTPIP1 and pyrin biology. We document that PSTPIP1, which has homology to membrane-deforming BAR proteins, forms homodimers and generates membrane-associated filaments in native and transfected cells. An extended FCH (Fes-Cip4 homology) domain in PSTPIP1 is necessary and sufficient for its self-aggregation. We further show that the PSTPIP1 filament network is dependent upon an intact tubulin cytoskeleton and that the distribution of this network can be modulated by pyrin, indicating that this is a dynamic structure. Finally, we demonstrate that pyrin can recruit PSTPIP1 into aggregations (specks) of ASC, another pyrin binding protein. ASC specks are associated with inflammasome activity. PSTPIP1 molecules with PAPA-associated mutations are recruited by pyrin to ASC specks with particularly high efficiency, suggesting a unique mechanism underlying the robust inflammatory phenotype of PAPA syndrome.
Waite
Andrea L
AL
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA.
Schaner
Philip
P
Richards
Neil
N
Balci-Peynircioglu
Banu
B
Masters
Seth L
SL
Brydges
Susannah D
SD
Fox
Michelle
M
Hong
Arthur
A
Yilmaz
Engin
E
Kastner
Daniel L
DL
Reinherz
Ellis L
EL
Gumucio
Deborah L
DL
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D000071198:Pyrin
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C000604192:MEFV protein, human; C486382:PSTPIP1 protein, human; D000071198:Pyrin