Localization of catecholamine fluorescence and retrogradely transported horseradish peroxidase within the same nerve cell.
A technique that enables both catecholamines (CA) and horseradish peroxidase (HRP) to be localized in the same cell is described. HPR was injected into the spinal cord and three days later rabbits were perfused with a mixture of 4% formaldehyde and 0.5% glutaraldehyde to produce a fluorescence reaction in CA-containing cells. Subsequently, HRP was localized in the same neurons using tetramethylbenzidine.
Blessing
W W
WW
Centre for Neuroscience, The Flinders University of South Australia, Bedford Park S.A. 5042 Australia.
Furness
J B
JB
Costa
M
M
Chalmers
J P
JP