Selective accumulation of harmful compounds by the DNA-inorganic hybrid-immobilized glass bead.
Previously, we reported the DNA-inorganic hybrid material including double-stranded DNA by mixing the aqueous DNA solution and silane coupling reagents. Here, we immobilized the DNA-inorganic hybrid material onto the glass bead and prepared the DNA-immobilized glass bead column. The DNA-immobilized glass beads were stable in water and the amount of eluted DNA from the DNA-glass beads did not change for more than 1 week. Additionally, this DNA-immobilized column selectively accumulated the harmful compounds with the planar structure, such as dioxin- and polychlorinated biphenyl (PCB)-derivatives, and these accumulation percentages were 50-70%. Furthermore, the DNA-immobilized glass bead was recycled nine times by the application of ethanol solution and the accumulative ratio was maintained at more than 60% and did not appear to be decreasing. Therefore, these DNA-columns might have a potential for the selective removal and separation of DNA-intercalating molecules and harmful compounds with the planar structure from experimental or industrial drainages.
Yamada
Masanori
M
Department of Chemistry, Faculty of Science, Okayama University of Science, Okayama 700-0005, Japan. myamada@chem.ous.ac.jp
Hamai
Akari
A
D004247:DNA / Q000737:chemistry*; D004147:Dioxins / Q000302:isolation & purification*; D005898:Glass / Q000737:chemistry*; D007364:Intercalating Agents / Q000302:isolation & purification*; D011078:Polychlorinated Biphenyls / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry
D004147:Dioxins; D007364:Intercalating Agents; D014867:Water; D004247:DNA; D011078:Polychlorinated Biphenyls