[Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis].
The disadvantage of time-consuming and fussy steps of conventional silver-stained method for polyacrylamide gels is evident, and has become the choke point in its application. In this paper, a low-background and high-resolution contracted silver-stained method for polyacrylamide gels was established. Compared with the conventional banding method, this contracted method has fewer steps and needs fewer reagents, in particular, lower NaOH concentration. It is rapid and economic, especially for mass silver-staining.
Gao
Dong
D
The National Center for Agricultural Biodiversity, Ministry of Education Key Laboratory of Agricultural Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming, China. gaodong521@yahoo.com.cn
DU
Fei
F
Zhu
You-Yong
YY
D019411:Clinical Laboratory Techniques; D004247:DNA / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016622:Silver Staining / Q000379:methods*
D007202:Indicators and Reagents; D004247:DNA