In vivo imaging of cell morphology and cellular processes in Caenorhabditis elegans, using non-linear phenomena.
We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using non-linear microscopy. Complementary information about the anatomy of the nematode was collected by implementing a combination of two photon excitation fluorescence (TPEF), second and third harmonic generation (SHG and THG) image contrast modes on the same microscope. Three-dimensional (3D) reconstructions of TPEF, SHG and THG images were also performed. Moreover, THG imaging technique has been tested as a potential, novel, non-destructive diagnostic tool for monitoring cellular processes in vivo, such as neuronal degeneration.
Filippidis
G
G
Institute of Electronic Structure and Laser, Foundation of Research and Technology-Hellas, P.O. Box 1385, 71110 Heraklion, Crete, Greece. filip@iesl.forth.gr
Gualda
E J
EJ
Mari
M
M
Troulinaki
K
K
Fotakis
C
C
Tavernarakis
N
N
D000818:Animals; D017173:Caenorhabditis elegans / Q000033:anatomy & histology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*