Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC.
RP HPLC-UV-MS was optimized for simultaneous determination of several prominent flavonoids (quercetin, naringenin, naringin, myricetin, rutin and kaempferol) using a commercially available monolithic column and isocratic elution. The concentration of organic modifier (methanol and ACN) was tested in order to obtain the best resolution. The LODs for a UV detector at 254 nm were comprised between 0.1 microg/mL for myricetin and quercetin and 0.5 microg/mL for rutin, naringin and kaempferol with the precision in the range of 1.5-3.0% RSD. MS detection with multiply reaction monitoring (MRM) offers a considerable gain in selectivity as well as sensitivity LODs for flavonoids were in the range of 1-20 ng/mL. The developed method was used for monitoring the content of some flavonoids in tomatoes during their maturation process.
Biesaga
Magdalena
M
Department of Chemistry, University of Warsaw, Warsaw, Poland.
Ochnik
Urszula
U
Pyrzynska
Krystyna
K
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D044949:Kaempferols / Q000032:analysis; D018551:Solanum lycopersicum / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis; D011794:Quercetin / Q000032:analysis; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis; D012680:Sensitivity and Specificity
D044950:Flavanones; D005419:Flavonoids; D044949:Kaempferols; D010936:Plant Extracts; D012431:Rutin; C006552:kaempferol; C040015:myricetin; D011794:Quercetin; C005273:naringenin; C005274:naringin; D000432:Methanol