Jiangella alba sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis.
A novel actinomycete strain, YIM 61503T, isolated from the stem of Maytenus austroyunnanensis, was characterized by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 61503T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 98.8 and 98.6% to Jiangella alkaliphila D8-87T and Jiangella gansuensis YIM 002T. The chemotaxonomic properties of strain YIM 61503T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid and MK-9(H4) was the predominant menaquinone. The major fatty acids were anteiso-C15:0, iso-C16:0, iso-C14:0, C17:1omega8c and anteiso-C17:0. The DNA G+C content was 71.9 mol%. Strain YIM 61503T was phenotypically distinct from recognized Jiangella species and was shown to belong to a separate genomic species based on DNA-DNA hybridization results. Thus, strain YIM 61503T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella alba sp. nov. is proposed. The type strain is YIM 61503T (=DSM 45237T=CCTCC AA 208023T).
Qin
Sheng
S
The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Zhao
Guo-Zhen
GZ
Li
Jie
J
Zhu
Wen-Yong
WY
Xu
Li-Hua
LH
Li
Wen-Jun
WJ
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D029755:Maytenus / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D018547:Plant Stems / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid