title: Glycosaminoglycan degradation-inhibitory lactic acid bacteria ameliorate 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice.
abstract: To evaluate the effects of lactic acid bacteria (LAB) in inflammatory bowel diseases (IBD), we measured the inhibitory effect of several LAB isolated from intestinal microflora and commercial probiotics against the glycosaminoglycan (GAG) degradation by intestinal bacteria. Bifidobacterium longum HY8004 and Lactobacillus plantarum AK8-4 exhibited the most potent inhibition. These LAB inhibited colon shortening and myeloperoxidase production in 2,4,6- trinitrobenzenesulfonic acid (TNBS)-induced experimental colitic mice. These LAB also blocked the expression of the proinflammatory cytokines, IL-1beta and TNF-alpha, as well as of COX-2, in the colon. LAB also blocked activation of the transcription factor, NF-kappaB, and expression of TLR-4 induced by TNBS. In addition, LAB reduced the TNBS-induced bacterial degradation activities of chondroitin sulfate and hyaluronic acid. These findings suggest that GAG degradation-inhibitory LAB may improve colitis by inhibiting inflammatory cytokine expression via TLR-4-linked NF-kB activation and by inhibiting intestinal bacterial GAG degradation.
authors: Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Korea.
authors: Bomi
authors: B
authors: Lee
authors: Jung-Hee
authors: JH
authors: Lee
authors: Hye-Sung
authors: HS
authors: Lee
authors: Eun-Ah
authors: EA
authors: Bae
authors: Chul-Sung
authors: CS
authors: Huh
authors: Young-Tae
authors: YT
authors: Ahn
authors: Dong-Hyun
authors: DH
authors: Kim
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000382:microbiology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D019936:Probiotics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D006025:Glycosaminoglycans; D053583:Interleukin-1beta; D016328:NF-kappa B; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D014302:Trinitrobenzenesulfonic Acid; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2