Whole-genome microarrays: applications and technical issues.
DNA microarrays have become a mainstream tool in experimental plant biology. The constant improvements in the technological platforms have enabled the development of the tiling DNA microarrays that cover the whole genome, which in turn catalyzed the wide variety of creative applications of such microarrays in the areas as diverse as global studies of genetic variation, DNA-binding proteins, DNA methylation, and chromatin and transcriptome dynamics. This chapter attempts to summarize such applications as well as discusses some technical and strategic issues that are particular to the use of tiling microarrays.
Gregory
Brian D
BD
Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Belostotsky
Dmitry A
DA
D047369:Chromatin Immunoprecipitation / Q000379:methods; D002874:Chromosome Mapping / Q000379:methods; D004722:Endoribonucleases / Q000037:antagonists & inhibitors / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics / Q000502:physiology; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000037:antagonists & inhibitors / Q000502:physiology; D020365:RNA Helicases / Q000037:antagonists & inhibitors / Q000502:physiology; D020871:RNA Stability / Q000502:physiology; D017422:Sequence Analysis, DNA / Q000379:methods
D009097:Multienzyme Complexes; C111244:degradosome; D011117:Polyribonucleotide Nucleotidyltransferase; D004722:Endoribonucleases; D020365:RNA Helicases