Frequent promoter hypermethylation and transcriptional downregulation of BTG4 gene in gastric cancer.
The BTG4 gene belongs to the BTG family of genes endowed with antiproliferative properties. In this study, we have found that BTG4 undergoes promoter CpG island hypermethylation-associated inactivation in gastric cancer and 5'-aza-2'-deoxycytidine (DAC) treatment restores BTG4 expression. We also found BTG4 levels were significantly reduced in primary gastric cancer but not in normal gastric tissues. BTG4 reexpression in gastric cancer causes growth inhibition of colony assays and nude mice. Taken together, our data support BTG4 as a candidate tumor suppressor gene that is epigenetically silenced in the majority of gastric cancers.
Dong
Wenjie
W
Department of Gastroenterology, Rui-jin Hospital, Shanghai Jiao Tong University, 197 Rui-jin Road, Shanghai, China.
Tu
Shuiping
S
Xie
Jing
J
Sun
Pinghu
P
Wu
Yunlin
Y
Wang
Lifu
L
D000818:Animals; D017209:Apoptosis; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D013274:Stomach Neoplasms / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
C415579:BTG4 protein, human; D018797:Cell Cycle Proteins; D016159:Tumor Suppressor Protein p53