title: Role of annexin A2 in cellular entry of rabbit vesivirus.
abstract: The mechanisms of calicivirus attachment and internalization are not well understood, mainly due to the lack of a reliable cell-culture system for most of its members. In this study, rabbit vesivirus (RaV) virions were shown to bind annexin A2 (ANXA2) in a membrane protein fraction from HEK293T cells, using a virus overlay protein-binding assay and matrix-assisted laser desorption/ionization time-of-flight analysis. A monoclonal anti-ANXA2 antibody and small interfering RNA-mediated knockdown of ANXA2 expression in HEK293T cells reduced virus infection significantly, further supporting the role of ANXA2 in RaV attachment and/or internalization.
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: Salomé
authors: S
authors: González-Reyes
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: Alberto
authors: A
authors: García-Manso
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: Gloria
authors: G
authors: Del Barrio
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: Kevin P
authors: KP
authors: Dalton
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: Lorenzo
authors: L
authors: González-Molleda
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: José
authors: J
authors: Arrojo-Fernández
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: Inés
authors: I
authors: Nicieza
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.
authors: Francisco
authors: F
authors: Parra
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D055785:Gene Knockdown Techniques; D006801:Humans; D011485:Protein Binding; D011817:Rabbits; D011991:Receptors, Virus / Q000037:antagonists & inhibitors / Q000378:metabolism*; D029342:Vesivirus / Q000502:physiology*; D053586:Virus Internalization
chemical_list: D017306:Annexin A2; D000911:Antibodies, Monoclonal; D011991:Receptors, Virus