title: A field theoretical restoration method for images degraded by non-uniform light attenuation : an application for light microscopy.
abstract: Microscopy has become a de facto tool for biology. However, it suffers from a fundamental problem of poor contrast with increasing depth, as the illuminating light gets attenuated and scattered and hence can not penetrate through thick samples. The resulting decay of light intensity due to attenuation and scattering varies exponentially across the image. The classical space invariant deconvolution approaches alone are not suitable for the restoration of uneven illumination in microscopy images. In this paper, we present a novel physics-based field theoretical approach to solve the contrast degradation problem of light microscopy images. We have confirmed the effectiveness of our technique through simulations as well as through real field experimentations.
authors: Imaging Informatics Division, Bioinformatics Institute, Singapore. urlleehk@bii.a-star.edu.sg
authors: Hwee Kuan
authors: HK
authors: Lee
authors: Mohammad Shorif
authors: MS
authors: Uddin
authors: Shvetha
authors: S
authors: Sankaran
authors: Srivats
authors: S
authors: Hariharan
authors: Sohail
authors: S
authors: Ahmed
mesh_terms: D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D008027:Light; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008962:Models, Theoretical; D009474:Neurons / Q000473:pathology; D055095:Optics and Photonics; D017785:Photons; D010825:Physics / Q000379:methods; D012542:Scattering, Radiation; D013234:Stem Cells / Q000473:pathology