Detection of fluorescent-labeled probes at subpicomolar concentrations by magnetic modulation.
A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1 x 10(-13) M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3 x 10(-14) M.
Danielli
Amos
A
Department of Physical Electronics, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel. amosd@post.tau.ac.il
Arie
Ady
A
Porat
Noga
N
Ehrlich
Marcelo
M
D015342:DNA Probes / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D008280:Magnetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D015336:Molecular Probe Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015342:DNA Probes