Immunoprecipitation of methylated DNA.
DNA methylation contributes to the regulation of long-term gene repression by enabling the recruitment of transcriptional repressor complexes to methylated cytosines. Several methods for detecting DNA methylation at the gene-specific and genome-wide levels have been developed. Methylated DNA immunoprecipitation, or MeDIP, consists of the selective immunoprecipitation of methylated DNA fragments using antibodies to 5-methylcytosine. The genomic site of interest can be detected by PCR, hybridization to DNA arrays, or by direct sequencing. This chapter describes the MeDIP protocol and quality control tests that should be performed throughout the procedure.
Sørensen
Anita L
AL
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Collas
Philippe
P
D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D019175:DNA Methylation / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods
D004247:DNA