title: [Preparation and preliminary application of rabbit polyclonal antibodies against intracellular peptides of human platelet glycoprotein GPIbalpha].
abstract: OBJECTIVE
  To prepare rabbit polyclonal antibodies against intracellular peptides of human platelet glycoprotein GPIbalpha.
  
  
  METHODS
  Two peptides corresponding to human platelet GPIbalpha C-terminus were synthesized and purified by high-performance liquid chromatography (HPLC). The peptides were cross-linked with keyhole limpet hemocyanin (KLH). Two New Zealand white rabbits were immunized with conjugated peptides for 3 times. The polyclonal antibodies were purified by Ammonium Sulfate Precipitation and identified by dot blotting and ELISA. GPIbalpha intracellular peptides phosphorylation was tested with these polyclonal antibodies by ELISA.
  
  
  RESULTS
  The titers of the two polyclonal antibodies against the GPIbalpha C-terminus peptides were 1:32 000 and 1:64 000 respectively and both of these antibodies reacted with purified GPIbalpha.
  
  
  CONCLUSIONS
  Two rabbit polyclonal antibodies against C-terminal peptides of human platelet GPIbalpha have been prepared successfully, providing a way for the preparation of these kinds of antibody. Both phosphorylation and dephosphorylation states exist in the intracellular peptide of human platelets.
authors: Hematology and Cell Biology Laboratory, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
authors: Yan-Hong
authors: YH
authors: Yuan
authors: Jia-Rui
authors: JR
authors: Kang
authors: Rong
authors: R
authors: Yan
authors: Hong
authors: H
authors: Cheng
authors: Feng-Yuan
authors: FY
authors: Zhuang
authors: Yue-Dan
authors: YD
authors: Wang
authors: Ke-Sheng
authors: KS
authors: Dai
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D010768:Phosphoserine / Q000737:chemistry; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000276:immunology; D011817:Rabbits
chemical_list: D000906:Antibodies; D008562:Membrane Glycoproteins; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; D010768:Phosphoserine