Qingkailing injection attenuates apoptosis and neurologic deficits in a rat model of intracerebral hemorrhage.
OBJECTIVE
Traditional Chinese herb Angong Niuhuang Pill (AGNHP) is a famous preparation for neurological diseases; Qingkailing injection (QKL), an extract of AGNHP has similar clinical applications. This investigation was designed to further elucidate the neuroprotective effect of QKL on intracerebral hemorrhage (ICH).


METHODS
ICH was produced in adult Sprague-Dawley rats by injection of collagenase IV. Three incremental doses of QKL injection including low-(0.5 ml/kg), moderate-(1 ml/kg) and high-dosage (2 ml/kg) were administered twice, 3 and 12h following ICH. TUNEL and caspase-3 activity were measured at 1d after ICH, and apomorphine-induced rotation was evaluated at 1d, 7d, 14 d and 28 d.


RESULTS
Administration of high-dose QKL inhibited TUNEL positive cells (p<0.05) and caspase-3 activity (p<0.05) at 1d following ICH, and reduced apomorphine-induced rotation at 1d (p<0.01), 7d, 14 d and 28 d (p<0.05), compared with the controls. However, QKL in a low or moderate dose had no such effect.


CONCLUSIONS
QKL reduced brain damage of intracerebral hemorrhage through inhibiting apoptosis, which suggested a potential intervention for ICH patients.
Lv
Lei
L
Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, PR China.
Liu
Yi
Y
Shi
Hui-fen
HF
Dong
Qiang
Q
D000818:Animals; D001058:Apomorphine; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects*; D061945:Caspase Inhibitors; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000188:drug therapy*; D017364:Collagenases; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D007275:Injections, Intravenous; D019684:Magnoliopsida; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley
D061945:Caspase Inhibitors; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C504928:Qingkailing; D017364:Collagenases; D001058:Apomorphine