Protecting effect of phytoncide solution, on normal human dermal fibroblasts against reactive oxygen species.
Four types of phytoncide solutions (A-Type, AB-Type, D-Type and G-Type) was evaluated for reduction of cell damage induced by oxidative stress, ultraviolet A (UVA), ultraviolet B (UVB), hydroxyperoxide (H2O2) and t-butyl-hydroperoxide (t-BHP); stimulation of collagen synthesis against UVA irradiation; and inhibition of matrix metalloproteinase-1 (MMP-1) activity induced by UVA in human normal dermal fibroblasts and human reconstituted skin model. The A-Type, AB-Type, D-Type and G-Type of phytoncide solutions pretreatment resulted in significant protection against cell damage induced by UVB, UVA, H2O2 and t-BHP. The amount of type I collagen following UVA irradiation was increased by treatment with phytoncide solutions in a concentration-dependent manner. On the other hand, phytoncide solutions also suppressed the excess MMP-1 irradiated UVA in a concentration-dependent manner. These effects of G-type solution were superior to those of other types solutions.
Fujimori
Hiroaki
H
Department of Pharmacy, School of Pharmacy, Kinki University.
Hisama
Masayoshi
M
Shibayama
Hiroharu
H
Iwaki
Masahiro
M
D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D006109:Poaceae / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012867:Skin / Q000166:cytology*; D012996:Solutions; D014466:Ultraviolet Rays; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012996:Solutions; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D020781:Matrix Metalloproteinase 1