title: Expression of MAGE-A1, MAGE-A3/4 and NY-ESO-1 cancer-testis antigens in fetal testis.
abstract: Immunohistochemical expression of MAGE-A1, MAGE-A3/4 and NY-ESO-1/LAGE-1 cancer testis antigens (CTA) was assessed in 24 fetal testes from 15th to 36th week of gestation. Three monoclonal antibodies were used for immunohistochemical staining: 77B recognizing MAGE-A1, 57B recognizing multiple MAGE-A CTA, and D8.38 recognizing NY-ESO-1/LAGE-1. Expression of MAGE-A1 was not observed in fetal testis samples, whereas multi-MAGE-A and NY-ESO-1/LAGE-1 specific reagents stained pro-spermatogonia in all samples with different expression levels during the period of fetal development observed. Significant expression of MAGE-A3/4 and almost continuous expression of NY-ESO-1 in fetal testes after 22nd week of gestation suggested their important role in the development of sex cords and pro-spermatogonia in particular.
authors: University Department of Urology, Zagreb University Hospital Center, Kispatićeva 12, HR-10000 Zagreb, Croatia. thudolin@hotmail.com
authors: Tvrtko
authors: T
authors: Hudolin
authors: Zeljko
authors: Z
authors: Kastelan
authors: Danijel
authors: D
authors: Derezić
authors: Nikolina
authors: N
authors: Basić-Jukić
authors: Giulio
authors: G
authors: Cesare Spagnoli
authors: Antonio
authors: A
authors: Juretić
authors: Davor
authors: D
authors: Jezek
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011247:Pregnancy; D011471:Prostatic Neoplasms / Q000378:metabolism*; D013737:Testis / Q000196:embryology / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; C072013:MAGEA3 protein, human; C095204:MAGEA4 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins