Genetic variability of Blastocystis sp. isolates obtained from cancer and HIV/AIDS patients.
This represents the first study to determine the genetic diversity of Blastocystis sp. among cancer and HIV/AIDS patients. Forty Blastocystis sp. isolates obtained from 20 cancer and 20 HIV/AIDS patients were genotyped by PCR using seven pairs of known sequenced-tagged site primers. Out of the 40 isolates, 38 were identified as one of the known genotypes and two isolates were negative with all the STS primers. Blastocystis sp. subtype 3 which is reported to be associated with disease was found to be predominant among the study subjects.
Tan
T C
TC
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Ong
S C
SC
Suresh
K G
KG
D000818:Animals; D016844:Blastocystis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016776:Blastocystis Infections / Q000469:parasitology*; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D009369:Neoplasms / Q000150:complications*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D017931:DNA Primers; D016054:DNA, Protozoan