Construction of a protein library of Arabidopsis transcription factors using a wheat cell-free protein production system and its application for DNA binding analysis.
We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5'-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.
Nozawa
Akira
A
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University, Matsuyama, Ehime, Japan.
Matsubara
Yuko
Y
Tanaka
Yoshinori
Y
Takahashi
Hirotaka
H
Akagi
Tatsuya
T
Seki
Motoaki
M
Shinozaki
Kazuo
K
Endo
Yaeta
Y
Sawasaki
Tatsuya
T
D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000096:biosynthesis / Q000378:metabolism; D002474:Cell-Free System; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019151:Peptide Library; D014157:Transcription Factors / Q000096:biosynthesis* / Q000378:metabolism*; D014908:Triticum / Q000166:cytology / Q000378:metabolism*
D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D019151:Peptide Library; D014157:Transcription Factors; D004247:DNA