Design and characterization of a maltose binding protein-linked growth factor for matrix engineering.
The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm(2) at 100 microg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.
Han
Min
M
Division of Life Sciences, Biomaterials Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, 130-650, Korea.
Park
In Su
IS
Kim
Soo Hyun
SH
Kim
Byung Soo
BS
Kim
Sang-Heon
SH
D000327:Adsorption; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D003460:Crystallization; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D058619:Maltose-Binding Proteins; D011137:Polystyrenes; D015202:Protein Engineering; D011791:Quartz; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism*; D015203:Reproducibility of Results; D012995:Solubility; D013499:Surface Properties; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism*
D002352:Carrier Proteins; D055767:Immobilized Proteins; D058619:Maltose-Binding Proteins; D011137:Polystyrenes; D011993:Recombinant Fusion Proteins; D042442:Vascular Endothelial Growth Factors; D011791:Quartz