Wasp mastoparans follow the same mechanism as the cell-penetrating peptide transportan 10.
We have been examining the mechanism and kinetics of the interactions of a selected set of peptides with phospholipid membranes in a quantitative manner. This set was chosen to cover a broad range of physical-chemical properties and cell specificities. Mastoparan (masL) and mastoparan X (masX) are two similar peptides from the venoms of the wasps Vespula lewisii and Vespa xanthoptera, respectively, and were chosen to complete the set. The rate constants for masX association with and dissociation from membranes are reported here for the first time. The kinetics of dye efflux induced by both mastoparans from phospholipid vesicles were also examined and quantitatively analyzed. We find that masL and masX follow the same graded kinetic model that we previously proposed for the cell-penetrating peptide transportan 10 (tp10), but with different parameters. This comparison is relevant because tp10 is derived from masL by addition of a mostly nonpolar segment of seven residues at the N-terminus. Tp10 is more active than the mastoparans toward phosphatidylcholine vesicles, but the mastoparans are more sensitive to the effect of anionic lipids. Furthermore, the Gibbs free energies of binding and insertion of the peptides calculated using the Wimley-White transfer scales are in good agreement with the values derived from our experimental data and are useful for understanding peptide behavior.
Yandek
Lindsay E
LE
Department of Chemistry and Biochemistry, University of North Carolina Wilmington, Wilmington, North Carolina 28403, USA.
Pokorny
Antje
A
Almeida
Paulo F F
PF
D000595:Amino Acid Sequence; D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D019004:Galanin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry; D020033:Protein Isoforms / Q000737:chemistry* / Q000378:metabolism; D011726:Pyridinium Compounds / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D014862:Wasp Venoms / Q000737:chemistry* / Q000378:metabolism; D014863:Wasps / Q000737:chemistry*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D036341:Intercellular Signaling Peptides and Proteins; D008051:Lipid Bilayers; D008563:Membrane Lipids; D009281:Naphthalenes; D010455:Peptides; D010743:Phospholipids; D020033:Protein Isoforms; D011726:Pyridinium Compounds; D011993:Recombinant Fusion Proteins; D014862:Wasp Venoms; C110068:transportan; C046466:N,N'-4-xylylenebis(pyridinium); C024098:6-carboxyfluorescein; C037500:mastoparan; D019004:Galanin; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid