title: Characterization of genotypes of Enterocytozoon bieneusi in immunosuppressed and immunocompetent patient groups.
abstract: A retrospective phylogenetic analysis was performed on isolates of Enterocytozoon bieneusi to characterize the genotypes in different patient cohorts. Fifty-seven isolates, collected from patients living in Malawi and the Netherlands, were classified by age and immune status of the hosts. Sequence analysis of the internal transcribed spacer (ITS) region identified 16 genotypes; nine have not previously been described. Genotypes K and D were most prevalent among patient groups, whereas genotype C was restricted to transplantation patients receiving immunosupressives and genotype B showed a predisposition toward patients living with HIV/AIDS. Different genotypes showed more dispersion among isolates from Malawi compared with those from the Netherlands. A constructed map estimating the genealogy of the ITS region reveals a dynamic evolutionary process between the genotypes.
authors: Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands. R.ten_Hove@lumc.nl
authors: Robert J
authors: RJ
authors: ten Hove
authors: Lisette
authors: L
authors: Van Lieshout
authors: Mike B J
authors: MB
authors: Beadsworth
authors: M Arantza
authors: MA
authors: Perez
authors: Klaartje
authors: K
authors: Spee
authors: Eric C J
authors: EC
authors: Claas
authors: Jaco J
authors: JJ
authors: Verweij
mesh_terms: D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D021862:Enterocytozoon / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D008295:Malawi; D008297:Male; D016881:Microsporidiosis / Q000382:microbiology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009426:Netherlands; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal