title: Staining patterns in dry eye syndrome: rose bengal versus lissamine green.
abstract: OBJECTIVE
  To evaluate and compare corneal staining patterns of lissamine green (LG) versus rose bengal (RB) in patients with dry eye syndrome. Secondary objectives included addressing patient's comfort after instillation and to correlate disease severity with staining patterns.
  
  
  METHODS
  Randomized, comparative, crossover series. Patients with previous diagnosis of mild to moderate dry eye syndrome were divided in 2 groups regarding dye instillation order (group A: RB first; group B: LG first). Both dyes were applied in regular intervals, and a staining score (van Bijsterveld scale) was used to correlate and compare the results. Disease severity was determined by the Ocular Surface Disease Index. Comfort was evaluated by patient's answer in an objective questionnaire.
  
  
  RESULTS
  Sixty eyes of 30 consecutive patients (24 females and 6 males) were included. There was no statistical difference between groups regarding disease severity, sex, or age. LG and RB showed good clinical correlation in both groups (group A: r = 0.939, P < 0.001; group B: r = 0.915, P < 0.001). LG was better tolerated than RB (P = 0.003 in both groups). Overall, we found a low statistical correlation between disease severity and staining scores.
  
  
  CONCLUSIONS
  Both LG and RB showed similar staining patterns. RB was found to provide greater patient discomfort. There was no correlation between disease severity (addressed by the ocular surface disease index questionnaire) and staining patterns (measured by the van Bijsterveld scale).
authors: Department of Ophthalmology, State University of Campinas (UNICAMP), Campinas, Brazil. leo_m@terra.com.br
authors: Leonardo Martins
authors: LM
authors: Machado
authors: Rosane S
authors: RS
authors: Castro
authors: Bruno M
authors: BM
authors: Fontes
mesh_terms: D004396:Coloring Agents / Q000009:adverse effects; D018592:Cross-Over Studies; D015352:Dry Eye Syndromes / Q000175:diagnosis*; D005260:Female; D005456:Fluorescent Dyes / Q000009:adverse effects; D006801:Humans; D008086:Lissamine Green Dyes; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012395:Rose Bengal; D012720:Severity of Illness Index; D013194:Staining and Labeling / Q000379:methods; D011795:Surveys and Questionnaires
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D008086:Lissamine Green Dyes; D012395:Rose Bengal