Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA.
Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells.
Scanlon
Michael J
MJ
Cornell University, Ithaca, New York, USA.
Ohtsu
Kazuhiro
K
Timmermans
Marja C P
MC
Schnable
Patrick S
PS
D042282:Microdissection / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics
D018749:RNA, Plant