title: [Morphologic and immunophenotypic analysis of angioimmunoblastic T-cell lymphoma].
abstract: OBJECTIVE
  To study the morphologic and immunophenotypic features of angioimmunoblastic T-cell lymphoma (AITL), as well as the origin of the proliferative follicular dendritic cells (FDCs) in AITL.
  
  
  METHODS
  Immunohistochemical study for CD10, CXCL13, bcl-6 and CD21 was performed on 29 cases of AITL. Double immunostaining for bcl-6/CD3, CD10/CD21 and CD10/CD20 were also carried out. Cases of peripheral T-cell lymphoma, unspecified, extranodal NK/T-cell lymphoma, nasal-type, enteropathy-type T-cell lymphoma, anaplastic large cell lymphoma, subcutaneous panniculitis-like T-cell lymphoma and reactive lymphoid proliferation were selected as controls.
  
  
  RESULTS
  Amongst the 29 cases of AITL studied, 75.9% (22/29) showed aberrant expression of CD10, while all except one of the controlled cases were negative, 82.8% (24/29) of the AITL cases expressed CXCL13, while all cases of peripheral T-cell lymphoma, unspecified were negative. As for bcl-6 staining, although the highest percentage of bcl-6-positive cells was observed in AITL, the expression pattern was not useful in differentiating AITL from peripheral T-cell lymphoma, unspecified and lymphoid reaction. Besides, all cases of AITL demonstrated the characteristic proliferation of follicular dendritic cells. Two of the cases, which contained obvious germinal centers, had the follicular dendritic cell meshwork extending beyond the lymphoid follicles.
  
  
  CONCLUSIONS
  As compared with bcl-6, CD10 and CXCL13 are specific and sensitive markers in diagnosing AITL. Part of the proliferative FDCs in AITL may originate from the germinal centers.
authors: Department of Pathology, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.
authors: Yuan-yuan
authors: YY
authors: Zheng
authors: Gang
authors: G
authors: Chen
authors: Xiao-ge
authors: XG
authors: Zhou
authors: Shu-hong
authors: SH
authors: Zhang
authors: Yan-ning
authors: YN
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D054382:Chemokine CXCL13 / Q000378:metabolism*; D020566:Dendritic Cells, Follicular / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000378:metabolism / Q000473:pathology*; D016130:Immunophenotyping; D016411:Lymphoma, T-Cell, Peripheral / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000378:metabolism*; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000378:metabolism; D017464:Receptors, Complement 3d / Q000378:metabolism
chemical_list: C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; D051560:Proto-Oncogene Proteins c-bcl-6; D017464:Receptors, Complement 3d; D015260:Neprilysin