Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacterium.
A Gram-negative, motile bacterium, designated DCY36T, was isolated from soil of a ginseng field in South Korea and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain DCY36T belongs to genus Castellaniella in the family Alcaligenaceae of the class Betaproteobacteria. The 16S rRNA gene sequence similarities between strain DCY36T and the three recognized representatives of the genus, Castellaniella caeni Ho-11T, Castellaniella defragrans 54PinT and Castellaniella denitrificans NKNTAUT, were 98.4, 97.5 and 98.1%, respectively. Strain DCY36T exhibited relatively low levels of DNA-DNA relatedness with respect to these three species. The G+C content of the genomic DNA was 63.7 mol%. Strain DCY36T contained ubiquinone Q-8. The major fatty acids were C16:0 (27.4%), C18:1omega7c (16.9%) and summed feature 4 (C16:1omega7c and C15:0 iso 2-OH, 32.5%). On the basis of phenotypic and genotypic properties and phylogenetic distinctiveness, strain DCY36T (=KCTC 22398T=JCM 15515T) should be classified in the genus Castellaniella as the type strain of a novel species, for which the name Castellaniella ginsengisoli sp. nov. is proposed.
Kim
Myung Kyum
MK
Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Srinivasan
Sathiyaraj
S
Kim
Yeon-Ju
YJ
Yang
Deok-Chun
DC
D042401:Alcaligenaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D005894:Panax; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000032:analysis; D001617:beta-Glucosidase / Q000096:biosynthesis*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8; D001617:beta-Glucosidase