title: Expression of endostatin mediated by a novel non-viral delivery system inhibits human umbilical vein endothelial cells in vitro.
abstract: Ultrasound (US)-mediated microbubble destruction is recognized to have considerable potential for gene delivery, whereas, there is few report of its effect on enhancing liposomal transfection. In this study, we used pIRES2-EGFP/hES containing human endostatin (hES) cDNA as target gene to test the hypothesis that US exposure with microbubbles could improve liposomal transfection, and to investigate the possibility of intracellular delivery of ES gene using this method. Under the controlled US exposure condition with microbubbles, the plasmid:liposome was transferred into COS-7 cells. The transfection rate, the expression of endostatin and the inhibition effect of transfection-endostatin on endothelial cells were assessed. The results revealed that US-mediated microbubble destruction together with liposome could significantly enhance gene transfection without obvious cell damage. By this means, endostatin gene could be efficiently transferred into COS-7 cells and expressed. The transfection-endostatin could inhibit endothelial proliferation and migration, which suggests that the non-viral method might be useful in anti-angiogenesis therapy in the future.
authors: Department of Ultrasound, First Affiliated Hospital of Harbin Medical University, 150001, Harbin, China.
authors: Chunmei
authors: C
authors: Zhang
authors: Xueju
authors: X
authors: Zhang
authors: Chunbo
authors: C
authors: Liu
authors: Junfeng
authors: J
authors: Wang
authors: Xinghan
authors: X
authors: Liu
authors: Hulun
authors: H
authors: Li
authors: Jinghua
authors: J
authors: Wang
authors: Changjun
authors: C
authors: Wu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002522:Chlorocebus aethiops; D043169:Endostatins / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D014465:Ultrasonics; D014471:Umbilical Veins / Q000166:cytology*; D014780:Viruses / Q000235:genetics
chemical_list: D043169:Endostatins; D008081:Liposomes; D049452:Green Fluorescent Proteins