Effects of blood glucose fluctuation on skin biophysical properties, structure and antioxidant status in an animal model.
BACKGROUND
Intermittent blood-glucose fluctuation (BGF) can accelerate diabetes and its complications, but it is still unknown whether BGF can damage the skin of patients with diabetes.


OBJECTIVE
To investigate the effects of repetitive fluctuation in blood glucose concentration on the biophysical properties, structure and antioxidant capacity of diabetic mouse skin.


METHODS
Mice with alloxan-induced diabetes were injected with glucose three times daily to induce repetitive BGF. Six weeks later, skin elasticity and skin hydration were measured to evaluate skin water-holding capacity and contraction function. The histological changes of skin were also studied, using haematoxylin and eosin. In addition, we compared levels of blood sugar and advanced glycation end products (AGEs), the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) and hydroxyproline in the skin of normal mice and diabetic mice with or without BGF.


RESULTS
The diabetic mice with BGF displayed an 18.6% increase in blood sugar level, an 11.3% increase in AGEs content and a 13.1% increase in MDA content compared with the diabetic mice (P < 0.01). There was a greater reduction in skin elasticity, skin hydration, hydroxyproline content and SOD activity in the diabetic mice with BGF (P < 0.01). Histological examination showed that BGF enhanced skin lesions in diabetic mice.


CONCLUSIONS
Repetitive fluctuation in blood-glucose concentration produced a marked detrimental effect on skin structure and function in diabetic mice. The deleterious effects of BGF on skin appeared to due at least partly to the oxidative stress.
Ye
X
X
School of Life Science, East China Normal University Shanghai, China. xyye@bio.ecnu.edu.cn
Tong
Z
Z
Dang
Y
Y
Tu
Q
Q
Weng
Y
Y
Liu
J
J
Zhang
Z
Z
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001834:Body Water / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology; D004548:Elasticity; D005947:Glucose / Q000008:administration & dosage; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D008315:Malondialdehyde; D005947:Glucose; D006909:Hydroxyproline