Production of cyclodextrin glucanotransferase from an alkalophilic Bacillus sp. by pH-stat fed-batch fermentation.
Batch and fed-batch fermentation processes were employed to culture an alkalophilic Bacillus sp. for the production of cyclodextrin glucanotransferase (CGTase). CGTase production was repressed by glucose and induced by soluble starch. By fed-batch fermentation, a CGTase activity up to 56 unit ml(-1) with 65 g dry cells l(-1) were achieved. The CGTase activity and cell density were increased 360 and 510%, respectively, from those values achieved with batch fermentation.
Kuo
Chou-Chiang
CC
Department of Bioengineering, Tatung University, Taipei, Taiwan.
Lin
Ching-An
CA
Chen
Jing-Yi
JY
Lin
Ming-Tse
MT
Duan
Kow-Jen
KJ
D000468:Alkalies / Q000378:metabolism*; D001407:Bacillus / Q000166:cytology / Q000201:enzymology*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D005964:Glucosyltransferases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration
D000468:Alkalies; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase