Selective separation and determination of primidone in pharmaceutical and human serum samples using molecular imprinted polymer-electrospray ionization ion mobility spectrometry (MIP-ESI-IMS).
Application of electrospray ionization ion mobility spectrometry (ESI-IMS) as the detection technique for separation method based on molecular imprinted polymer (MIP) was investigated and evaluated. The method is exhaustively validated, including sensitivity, selectivity, recovery, reproducibility, and column capacity. The linear dynamic range of 0.02-2.00 microg mL(-1) was obtained for primidone analysis with ESI-IMS. The recovery of drug analyzed was calculated to be above 90% and the relative standard deviation (RSD), was below 3% for all experiments. Various real samples were analyzed with the coupled techniques, and the results obtained revealed the efficient clean-up of the samples using MIP separation before the analysis by ESI-IMS as a detection technique.
Rezaei
B
B
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Jafari
M T
MT
Khademi
R
R
D000927:Anticonvulsants / Q000032:analysis* / Q000097:blood* / Q000302:isolation & purification; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D054802:Molecular Imprinting; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011324:Primidone / Q000032:analysis* / Q000097:blood* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors
D000927:Anticonvulsants; D004364:Pharmaceutical Preparations; D011108:Polymers; D012997:Solvents; D011324:Primidone