title: Evaluation of excreted/secreted antigens derived from peritoneal of toxoplasma infected small mice to detect IgG against Toxoplasma.
abstract: The present study, evaluated, using the components of peritoneal fluid of infected mice (as another source of E/SA), for the detection of Toxoplasma specific IgG in human serum. Peritoneal fluids of mice infected by intraperitoneal (IP) inoculation of Toxoplasma tachizoites were collected after 3 days and centrifuged at 750 x g for 15 min then the supernatant was precipitated with ammonium sulphate solution (40% saturated) and used as components containing E/SA. Forty nine uninfected (without anti-Toxoplasma antibodies) and thirty two positive (with IgG to Toxoplasma) human serum samples were selected (all sera were first tested by standard method for detection of IgG antibodies anti-T. gondii) then the sera were tested by ELISA using E/SA. The cut-off point with 95% confidences was found to be 0.78. Moreover, sensitivity and specificity of the method were determined to be 84 and 92%, respectively. The present results indicate that peritoneal exudates from mice infected with T. gondii may be used as a source of antigenic material for the detection of Toxoplasma-specific IgG and may be valuable for the development of new tools in the serodiagnosis of toxoplasmosis.
authors: Department of Microbiology and Immunology, Faculty of Medicine, Rafsanjan University of Medical Science, Rafsanjan, Iran.
authors: Seyed Ossein
authors: SO
authors: Abdollahi
authors: Mohammad Kazemi
authors: MK
authors: Arababadi
authors: Gholamhossein
authors: G
authors: Hassanshahi
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000378:metabolism; D001202:Ascitic Fluid / Q000737:chemistry / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D051379:Mice; D011247:Pregnancy; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G