title: Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions.
abstract: The "phiKMV-like viruses" comprise an important genus of T7 related phages infecting Pseudomonas aeruginosa. The genomes of these bacteriophages have localized single-strand interruptions (nicks), a distinguishing genomic trait previously thought to be unique for T5 related coliphages. Analysis of this feature in the newly sequenced phage phikF77 shows all four nicks to be localized on the non-coding DNA strand. They are present with high frequencies within the phage population and are introduced into the phage DNA at late stages of the lytic cycle. The general consensus sequence in the nicks (5'-CGACxxxxxCCTAoh pCTCCGG-3') was shown to be common among all phiKMV-related phages.
authors: School of Biological Sciences, The Queen's University of Belfast, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland. l.kulakov@qub.ac.uk
authors: Leonid A
authors: LA
authors: Kulakov
authors: Vladimir N
authors: VN
authors: Ksenzenko
authors: Michael G
authors: MG
authors: Shlyapnikov
authors: Vladimir V
authors: VV
authors: Kochetkov
authors: Antonio
authors: A
authors: Del Casale
authors: Christopher C R
authors: CC
authors: Allen
authors: Michael J
authors: MJ
authors: Larkin
authors: Pieter-Jan
authors: PJ
authors: Ceyssens
authors: Rob
authors: R
authors: Lavigne
mesh_terms: D016384:Consensus Sequence; D053904:DNA Breaks, Single-Stranded; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D017902:Podoviridae / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*
chemical_list: D004279:DNA, Viral