Colorimetric detection of microRNA and RNase H activity in homogeneous solution with cationic polythiophene derivative.
A colorimetric assay for convenient detection of miRNA and RNase H activity was established in a homogeneous and label-free manner based on conformational and colorimetric changes of a polythiophene derivative (PMNT) in the duplex of DNA/PMNT and triplex of DNA/miRNA/PMNT.
Zhang
Yali
Y
Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, PR China.
Li
Zhengping
Z
Cheng
Yongqiang
Y
Lv
Xuechong
X
D000042:Absorption; D001483:Base Sequence; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*; D016914:Ribonuclease H / Q000032:analysis / Q000378:metabolism*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013876:Thiophenes / Q000737:chemistry* / Q000378:metabolism*
D035683:MicroRNAs; D011108:Polymers; D012996:Solutions; D013876:Thiophenes; C066730:polythiophene; D004247:DNA; D016914:Ribonuclease H