title: Nanovesicles released by Dictyostelium cells: a potential carrier for drug delivery.
abstract: Nanovesicles released by Dictyostelium discoideum cells grown in the presence of the DNA-specific dye Hoechst 33342 have been previously shown to mediate the transfer of the dye into the nuclei of Hoechst-resistant cells. The present investigation extends this work by conducting experiments in the presence of hypericin, a fluorescent therapeutic photosensitizer assayed for antitumoral photodynamic therapy. Nanovesicles released by Dictyostelium cells exhibit an averaged diameter between 50 and 150 nm, as measured by transmission cryoelectron microscopy. A proteomic analysis reveals a predominance of actin and actin-related proteins. The detection of a lysosomal membrane protein (LIMP II) indicates that these vesicles are likely generated in the late endosomal compartment. The use of the hypericin-containing nanovesicles as nanodevices for in vitro drug delivery was investigated by fluorescence microscopy. The observed signal was almost exclusively located in the perinuclear area of two human cell lines, skin fibroblasts (HS68) and cervix carcinoma (HeLa) cells. Studies by confocal microscopy with specific markers of cell organelles, provided evidence that hypericin was accumulated in the Golgi apparatus. All these data shed a new light on in vitro drug delivery by using cell-released vesicles as carriers.
authors: CNRS, UMR7033, Université Pierre et Marie Curie, Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire, Genopole, F-91030 Evry, France.
authors: Françoise
authors: F
authors: Lavialle
authors: Sophie
authors: S
authors: Deshayes
authors: Florence
authors: F
authors: Gonnet
authors: Eric
authors: E
authors: Larquet
authors: Sergei G
authors: SG
authors: Kruglik
authors: Nicolas
authors: N
authors: Boisset
authors: Régis
authors: R
authors: Daniel
authors: Annette
authors: A
authors: Alfsen
authors: Irène
authors: I
authors: Tatischeff
mesh_terms: D000818:Animals; D000873:Anthracenes; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001562:Benzimidazoles; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004023:Dictyostelium / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry*; D011992:Endosomes / Q000737:chemistry* / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D010569:Perylene / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D040901:Proteomics
chemical_list: D000873:Anthracenes; D000970:Antineoplastic Agents; D001562:Benzimidazoles; D004337:Drug Carriers; D010569:Perylene; C004965:hypericin; C017807:bisbenzimide ethoxide trihydrochloride