In vivo photoacoustic molecular imaging with simultaneous multiple selective targeting using antibody-conjugated gold nanorods.
The use of gold nanorods for photoacoustic molecular imaging with simultaneous multiple targeting is reported. Multiple targeting is done by utilizing the tunable optical absorption property of gold nanorods. This technique allows multiple molecular signatures to be obtained by simply switching laser wavelength. HER2 and EGFR were chosen as the primary target molecules for examining two cancer cells, OECM1 and Cal27. Both in vitro and in vivo mouse model imaging experiments were performed, with contrast enhancement of up to 10 dB and 3.5 dB, respectively. The potential in improving cancer diagnosis is demonstrated.
Li
Pai-Chi
PC
Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan. paichi@cc.ee.ntu.edu.tw
Wang
Churng-Ren Chris
CR
Shieh
Dar-Bin
DB
Wei
Chen-Wei
CW
Liao
Chao-Kang
CK
Poe
Carolina
C
Jhan
Suwen
S
Ding
Ann-Ann
AA
Wu
Ya-Na
YN
D000818:Animals; D000906:Antibodies; D017076:Computer-Aided Design; D016503:Drug Delivery Systems / Q000379:methods; D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D066246:ErbB Receptors / Q000378:metabolism*; D006046:Gold; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D009062:Mouth Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration
D000906:Antibodies; D006046:Gold; D066246:ErbB Receptors