title: Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9.
abstract: Adeno-associated virus (AAV) serotype 9, which is under development for gene-delivery applications, shows significantly enhanced capsid-associated transduction efficiency in muscle compared with other AAV serotypes. With the aim of characterizing the structural determinants of this property, the purification, crystallization and preliminary X-ray crystallographic analyses of the AAV9 viral capsid are reported. The crystals diffracted X-rays to 2.8 A resolution using synchrotron radiation and belonged to the trigonal space group P3(2), with unit-cell parameters a = b = 251.0, c = 640.0 A. There are three complete viral capsids in the crystal unit cell. The orientation and position of the asymmetric unit capsid have been determined by molecular-replacement methods and structure determination is in progress.
authors: Department of Biochemistry and Molecular Biology, Center for Structural Biology, McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL 32610, USA.
authors: Matthew
authors: M
authors: Mitchell
authors: Hyun-Joo
authors: HJ
authors: Nam
authors: Adam
authors: A
authors: Carter
authors: Angela
authors: A
authors: McCall
authors: Chelsea
authors: C
authors: Rence
authors: Antonette
authors: A
authors: Bennett
authors: Brittney
authors: B
authors: Gurda
authors: Robert
authors: R
authors: McKenna
authors: Mark
authors: M
authors: Porter
authors: Yoshihisa
authors: Y
authors: Sakai
authors: Barry J
authors: BJ
authors: Byrne
authors: Nicholas
authors: N
authors: Muzyczka
authors: George
authors: G
authors: Aslanidi
authors: Sergei
authors: S
authors: Zolotukhin
authors: Mavis
authors: M
authors: Agbandje-McKenna
mesh_terms: D002213:Capsid / Q000737:chemistry / Q000648:ultrastructure; D003460:Crystallization; D018360:Crystallography, X-Ray; D000229:Dependovirus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D012703:Serotyping