title: Production and characterization of long-acting recombinant human albumin-EPO fusion protein expressed in CHO cell.
abstract: A long-lasting recombinant human albumin-linker-erythropoietin (EPO) is a human albumin gene fused to the N-terminal of EPO with a (GGSGG)(n)-repeated linker inserted between albumin and EPO. Albumin-EPO fusion genes were co-transfected with the dhfr gene. Albumin-EPO fusion protein has three kinds of sub-types (IALE, AD2LE, AD1LE). Albumin-EPO fusion protein was quantified with human EPO ELISA. The in vitro efficacy of albumin-EPO fusion protein was estimated using F-36E cell, and in vivo efficacy of albumin-EPO fusion protein was estimated using normocythemic mice (B6D2F1). We also determined the in vivo half-life in a Sprague-Dawley rat. A PLA program analysis result demonstrated that the albumin-EPO fusion protein IALE is about 7.8-fold more potent than rHuEPO in increasing the hematocrit of normal mice.
authors: Boryung Central Research Institute, Boryung Pharmaceutical Co. Ltd., Ansan 425-839, South Korea.
authors: Chan-Hi
authors: CH
authors: Joung
authors: Ju-Yeop
authors: JY
authors: Shin
authors: Jae-Kyung
authors: JK
authors: Koo
authors: Jin J
authors: JJ
authors: Lim
authors: Jin-Sang
authors: JS
authors: Wang
authors: Song-Jae
authors: SJ
authors: Lee
authors: Hyun-Kwang
authors: HK
authors: Tan
authors: Sang-Lin
authors: SL
authors: Kim
authors: Sang-Min
authors: SM
authors: Lim
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002452:Cell Count; D045744:Cell Line, Tumor; D002850:Chromatography, Gel; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D004921:Erythropoietin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006207:Half-Life; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins; D012156:Reticulocytes; D012709:Serum Albumin / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012709:Serum Albumin; D004921:Erythropoietin