title: Generation of single-cell suspensions from mouse neural tissue.
abstract: Within the nervous system, hundreds of neuronal and glial cell types have been described. Each specific cell type in the brain or spinal cord has a repertoire of cell surface molecules, or molecular determinants, through which it can be identified and characterized. Currently, robust cell identification and separation technologies require single-cell preparations to be generated while simultaneously limiting cell death and destruction of characteristic surface protein. The gentleMACS Dissociator, when used in combination with trypsin or papain-based dissociation kits, can effectively and gently dissociate brain tissue while preserving antigen epitopes and limiting cell loss. Standardized preparation of single-cell suspensions is achieved using C Tubes and optimized, preset gentleMACS Programs. Once generated, single-cell suspensions can be treated with monoclonal conjugates like Anti-Prominin-1 MicroBeads, which identify neural progenitors, or purified further using Myelin Removal Beads.
authors: Miltenyi Biotec GmbH, Germany.
authors: Sandra
authors: S
authors: Pennartz
authors: Sandy
authors: S
authors: Reiss
authors: Rebecca
authors: R
authors: Biloune
authors: Doris
authors: D
authors: Hasselmann
authors: Andreas
authors: A
authors: Bosio
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D051379:Mice