Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation.
The survey is aimed at critically reviewing information on the UVA-mediated oxidative reactions to cellular components with emphasis on DNA as the result of mostly photosensitized pathways. It appears clearly that UVA radiation is relatively much more efficient than UVB photons in inducing oxidative processes. The main UVA-induced oxidative degradation pathways of DNA are reported and discussed mechanistically. They are mostly rationalized in terms of a major contribution of singlet molecular oxygen ((1)O(2)) and to a lesser extent of hydroxyl radical ( OH), that in the latter case originates from Fenton-type reactions. This leads to the predominant formation of 8-oxo-7,8-dihydroguanine together with smaller amounts of oxidized pyrimidine bases and DNA strand breaks in UVA-irradiated cells.
Cadet
Jean
J
Laboratoire Lésions des Acides Nucléiques, SCIB-UMR-E no 3 (CEA/UJF), Institut Nanosciences et Cryogénie; CEA/Grenoble, 38054 Grenoble, France. jean.cadet@cea.fr
Douki
Thierry
T
Ravanat
Jean-Luc
JL
Di Mascio
Paolo
P
D000818:Animals; D002477:Cells / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000528:radiation effects*; D006801:Humans; D010084:Oxidation-Reduction; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
D017319:Photosensitizing Agents; D004247:DNA