The protective immune response against infectious bronchitis virus induced by multi-epitope based peptide vaccines.
Peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. To explore multi-epitope based peptide vaccines against infectious bronchitis virus (IBV), the immunogenic peptides were fused to the 3' terminal of glutathione S transferase gene (GST) and expressed in Escherichia coli. ELISA and Western blot analysis showed that the purified fusion proteins had excellent immune activity with chicken anti-IBV serum. During the vaccination course, the candidate peptide vaccines induced strong humoral and cellular response, and provided up to 80.0% immune protection, while all non-immunized chickens in the negative control group manifested obvious typical symptoms and died after virus challenge. Our finding provides a new way to develop multi-epitope based peptide vaccine against IBV.
Yang
Tai
T
Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, School of Life Sciences, Sichuan University, Chengdu, PR China.
Wang
Hong-Ning
HN
Wang
Xue
X
Tang
Jun-Ni
JN
Lu
Dan
D
Zhang
Yun-Fei
YF
Guo
Zi-Cheng
ZC
Li
Yu-Ling
YL
Gao
Rong
R
Kang
Run-Min
RM
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D049109:Cell Proliferation; D002645:Chickens / Q000276:immunology / Q000821:virology; D018352:Coronavirus Infections / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D056724:Immunity, Humoral / Q000276:immunology; D001351:Infectious bronchitis virus / Q000276:immunology* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology*
D000906:Antibodies; D000956:Antigens, Viral; D000939:Epitopes; D022223:Vaccines, Subunit