Studying the interaction of pirarubicin with DNA and determining pirarubicin in human urine samples: combining excitation-emission fluorescence matrices with second-order calibration methods.
In this paper, UV-vis spectroscopy and fluorescence were combined to study the binding of Calf thymus DNA (ct-DNA) with the anthacycline antibiotic drug pirarubicin (THP). Ethidium bromide (EB) as the fluorescence probe was used to study the competitive binding interactions of THP with DNA by excitation-emission fluorescence matrices (EEFMs) coupled with the parallel factor analysis (PARAFAC) and the alternating normalization-weighted error algorithm (ANWE) with the second-order advantage. All the results conformed that THP mainly bound with DNA by intercalation. Meanwhile, the two second-order calibration methods have been successfully applied to quantify THP in urine samples. Figures of merit were applied to compare the performance of the two methods. The results presented in this work showed that both the PARAFAC and ANWE methods were the convincing way to be applied in the complex biological systems even in the presence of uncalibrated interferences.
Zou
Hong-Yan
HY
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
Wu
Hai-Long
HL
Zhang
Yan
Y
Li
Shu-Fang
SF
Nie
Jin-Fang
JF
Fu
Hai-Yan
HY
Yu
Ru-Qin
RQ
D000465:Algorithms; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000652:urine; D001667:Binding, Competitive; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D004996:Ethidium / Q000737:chemistry; D005163:Factor Analysis, Statistical; D006801:Humans; D007364:Intercalating Agents / Q000378:metabolism*; D012815:Signal Processing, Computer-Assisted; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods*
D000903:Antibiotics, Antineoplastic; D007364:Intercalating Agents; D004317:Doxorubicin; D004247:DNA; C427203:calf thymus DNA; C027260:pirarubicin; D004996:Ethidium