title: Omentopexy enhances graft function in myocardial cell sheet transplantation.
abstract: Myocardial cell sheets (MCS) are a potentially valuable tool for tissue engineering aimed at heart regeneration. Several methods have recently been established for the fabrication of MCS. However, the lack of a sufficient blood supply has inhibited functional recovery of the MCS. To address this challenge, we combined MCS transplantation with omentopexy (OP), which utilizes omental tissue as a surgical flap. Rats were divided into five groups: sham, myocardial infarction (MI), MCS transplantation, OP, and MCS+OP. Histologic analysis revealed that MCS+OP drastically reversed MI-induced cardiac remodeling. Echocardiography revealed that MCS increased cardiac function, while OP had a synergistic beneficial effect with MCS transplantation. Immunofluorescence imaging showed that OP increased the survival of transplanted cardiomyocytes, and increased the blood supply through enhancement of angiogenesis and migration of small arteries into the MCS. Taken together, we concluded that OP is a promising strategy for the enhancement of graft function in MCS transplantation.
authors: Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan.
authors: Ryo
authors: R
authors: Suzuki
authors: Fumiyuki
authors: F
authors: Hattori
authors: Yuji
authors: Y
authors: Itabashi
authors: Masatoyo
authors: M
authors: Yoshioka
authors: Shinsuke
authors: S
authors: Yuasa
authors: Haruko
authors: H
authors: Manabe-Kawaguchi
authors: Mitsushige
authors: M
authors: Murata
authors: Shinji
authors: S
authors: Makino
authors: Kiyokazu
authors: K
authors: Kokaji
authors: Ryohei
authors: R
authors: Yozu
authors: Keiichi
authors: K
authors: Fukuda
mesh_terms: D000818:Animals; D004452:Echocardiography; D006085:Graft Survival; D009203:Myocardial Infarction / Q000601:surgery; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000000981:diagnostic imaging / Q000502:physiology* / Q000637:transplantation*; D009852:Omentum; D051381:Rats; D011923:Rats, Nude; D055647:Rats, Transgenic; D012038:Regeneration