title: Peptide-nucleic acids (PNAs) with pyrimido[4,5-d]pyrimidine-2,4,5,7-(1H,3H,6H,8H)-tetraone (PPT) as a universal base: their synthesis and binding affinity for oligodeoxyribonucleotides.
abstract: Peptide-nucleic acids (PNAs) including pyrimido[4,5-d]pyrimidine-2,4,5,7-(1H,3H,6H,8H)-tetraone (PPT) as a nucleobase were synthesized, and their binding affinity for the complementary oligodeoxyribonucleotides was investigated. We found that PNAs with one or two PPT(s) and natural nucleobases (i.e., adenine, cytosine, guanine, or thymine) have excellent binding affinity for oligodeoxyribonucleotides with complementary bases at the positions facing the natural nucleobases, and with adenine, cytosine, guanine, and thymine at the positions opposite PPT in PNAs. The binding affinity of the PPT-containing PNA is higher than or comparable to that of a PNA consisting of all complementary natural nucleobases, viz. a PNA with a suitable natural nucleobase in place of PPT in the PPT-containing PNA. Consequently, it was concluded that PPT serves as a useful universal base that can recognize all natural nucleobases.
authors: Graduate School of Information Science and CREST of JST, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
authors: Taisuke
authors: T
authors: Hirano
authors: Kenji
authors: K
authors: Kuroda
authors: Masanori
authors: M
authors: Kataoka
authors: Yoshihiro
authors: Y
authors: Hayakawa
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001483:Base Sequence; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011744:Pyrimidinones
chemical_list: D000596:Amino Acids; D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D011744:Pyrimidinones; C520194:pyrimido(4,5-d)pyrimidine-2,4,5,7-(1H,3H,6H,8H)-tetraone