[Effects of gingival fibroblasts transfected with human transform growth factor-beta1 gene on improving the periodontal tissue regeneration].
OBJECTIVE
To evaluate the effects of gingival fibroblasts (GF) transfected with hTGF-beta1 gene on improving the periodontal tissue regeneration for the repair of degree II artificial furcation defects.


METHODS
The gingival fibroblasts transfected with hTGF-beta1 gene was compounded to the cuttlebone-transformed nanometer hydroxyapatite (CBHA) material from the cuttlefish in vitro, the degree II furcation defects on the premolars of dogs were produced surgically, and the compound was to implanted into the defect (transfected group), and compared with the compound of periodontal ligament cells (PDLC) with nanometer HA material and the compound of untransfected GF with HA. The results were examined histologically 8 weeks after operation.


RESULTS
In the transfected group and the positive control group, more new attachment was found compared with the negative control (P < 0.01), and the NC, NB and NC of the transfected group and the positive control group were: (2.97 +/- 0.50), (4.29 +/- 0.26) and (4.73 +/- 0.06) mm; (3.09 +/- 0.26), (4.46 +/- 0.25) and (4.69 +/- 0.10) mm, respectively. There was no significant difference between the two groups (P > 0.05). Although the alveolar bone regeneration was found in the untransfected group [NB = (3.46 +/- 0.32) mm], the root resorption was observed. The tracing experiment showed that the transfected GF were found in the new alveolar bone and the periodontal membrane.


CONCLUSIONS
GF transfected with hTGF-beta1 gene can significantly improve the periodontal tissue regeneration in treatment of degree II furcation defects and is involved in the formation of the new alveolar bone and the new periodontal membrane.
Chu
Qing
Q
Department of Periodontology and Oral Medicine, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wu
Zhi-Fen
ZF
Xie
Guang-Yuan
GY
Wan
Ling
L
He
Hai-Li
HL
Liu
Ling-Xia
LX
D000818:Animals; D004285:Dogs; D005347:Fibroblasts; D005881:Gingiva / Q000166:cytology; D016557:Guided Tissue Regeneration, Periodontal; D008297:Male; D049329:Nanostructures; D010513:Periodontal Ligament; D023822:Tissue Engineering; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics*
D016212:Transforming Growth Factor beta