Effects of high cholesterol diet on newly generated cells in the dentate gyrus of C57BL/6N and C3H/HeN mice.
In this study, we observed and compared the effects of a high cholesterol diet (HCD) on cell proliferation and differentiation in the subgranular zone of the dentate gyrus of C57BL/6N (B6, susceptible strain) and C3H/HeN (C3H, resistant strain) mice. Ki67 (a marker for cell proliferation) positive cells) were significantly decreased in HCD-fed B6 mice compared to those in B6 (49.7%) and C3H mice fed a low cholesterol diet (LCD). In addition, doublecortin (DCX, a marker for cell differentiation or neuroblasts)-immunoreactive cells in HCD-fed B6 mice were significantly decreased compared to those in LCD-fed B6 and C3H mice. These results suggest that B6 strains are sensitive to HCD, which impairs cell proliferation and differentiation.
Kim
Il Yong
IY
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, South Korea.
Hwang
In Koo
IK
Choi
Ji Won
JW
Yoo
Ki-Yeon
KY
Kim
Yo Na
YN
Yi
Sun Shin
SS
Won
Moo-Ho
MH
Lee
In Se
IS
Yoon
Yeo Sung
YS
Seong
Je Kyung
JK
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002791:Cholesterol, Dietary / Q000008:administration & dosage* / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005260:Female; D006937:Hypercholesterolemia / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism
D002791:Cholesterol, Dietary; C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides