Short-term impact of dry olive mill residue addition to soil on the resident microbiota.
The short-term response of the resident soil bacterial and fungal communities to the addition of 5% (w/w) of either dry olive mill residue (DOR), DOR treated with Phlebia sp. (PTDOR) or DOR previously extracted with water (WEDOR) was investigated. As opposed to bacteria, the diversity of fungi increased upon the amendments as assessed by denaturing gradient gel electrophoresis of 18S rDNA. Over the first 30 days, phospholipid fatty acids analyses indicated a gradual decrease in the relative abundances of gram(+) bacteria (from 44.8% to 37.9%) and a concomitant increase of gram(-) bacteria (from 37.3% to 51.2%) in DOR-amended soil. A considerable increase in the fungal/bacterial ratio was observed after 7 days in DOR, WEDOR and PTDOR-amended soils with respect to the control (0.316, 0.165 and 0.265, respectively, vs. 0.011). The overall microbial activity was stimulated by the amendments as indicated by the higher activity levels of both dehydrogenase and fluorescein diacetate hydrolase. These results indicate that DOR at the application level examined is not toxic on soil microorganisms.
Sampedro
Inmaculada
I
Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Via San Camillo De Lellis s.n.c., 01100 Viterbo, Italy.
Giubilei
Mariangela
M
Cajthaml
Tomas
T
Federici
Ermanno
E
Federici
Federico
F
Petruccioli
Maurizio
M
D'annibale
Alessandro
A
D001419:Bacteria / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D004275:DNA, Ribosomal / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry; D031658:Olea / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012987:Soil; D012988:Soil Microbiology; D014867:Water / Q000378:metabolism
D004275:DNA, Ribosomal; D011134:Polysaccharides; D012337:RNA, Ribosomal, 18S; D012987:Soil; D014867:Water; D002244:Carbon; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin