Using transposon mutagenesis to find an alternative resolvase in an Escherichia coli cells lacking RuvABC.
This study was undertaken to identify an unknown resolvase in an E. coli strain lacking RuvABC (N4237) by using transposon mutagenesis. One out of 10000 clones was retained for further study as it was resistant to UV light and mitomycin C. The result of transductional mapping and PCR sequencing showed that Tn10kan inserted upstream of rusA gene and expression of this gene improved survival. Thus, results did not show the presence of new resolvase in E. coli cells.
Jaktaji
Razieh Pourahmad
RP
Department of Genetics, Faculty of Science, The University of Shahrekord, P.O. Box 115, Shahrekord, Iran.
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D016296:Mutagenesis; D045522:Recombinases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D045522:Recombinases; C061260:RuvB protein, Bacteria; C069806:ruvC protein, E coli; D004706:Endodeoxyribonucleases; C071059:Holliday junction DNA helicase, E coli; D004265:DNA Helicases