Calorie restriction alters mitochondrial protein acetylation.
Calorie restriction (CR) increases lifespan in organisms ranging from budding yeast through mammals. Mitochondrial adaptation represents a key component of the response to CR. Molecular mechanisms underlying this adaptation are largely unknown. Here we show that lysine acetylation of mitochondrial proteins is altered during CR in a tissue-specific fashion. Via large-scale mass spectrometry screening, we identify 72 candidate proteins involved in a variety of metabolic pathways with altered acetylation during CR. Mitochondrial acetylation changes may play an important role in the pro-longevity CR response.
Schwer
Bjoern
B
Program in Cellular and Molecular Medicine, Howard Hughes Medical Institute, The Children's Hospital, and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Eckersdorff
Mark
M
Li
Yu
Y
Silva
Jeffrey C
JC
Fermin
Damian
D
Kurtev
Martin V
MV
Giallourakis
Cosmas
C
Comb
Michael J
MJ
Alt
Frederick W
FW
Lombard
David B
DB
D000107:Acetylation; D000818:Animals; D031204:Caloric Restriction / Q000706:statistics & numerical data*; D006801:Humans; D008017:Life Expectancy; D008136:Longevity / Q000502:physiology*; D008322:Mammals / Q000254:growth & development / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D004718:Saccharomycetales / Q000254:growth & development / Q000502:physiology*
D011506:Proteins