Inhibitory effect of tea polyphenols on local tissue damage induced by snake venoms.
The methanolic extract of fresh tea leaves of Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae) venoms. These snake venom enzymes are responsible for the early effects of envenomation, such as local tissue damage and inflammation. The CS extract inhibited phospholipase A(2), proteases, hyaluronidase and L-amino acid oxidase in both venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities of the venoms in vivo. It is suggested that the inhibitory potential of the CS extract against local tissue damage induced by snake venoms may be attributed to complexation and chelation between the venom proteins and the phenolic contents of the extract.
Pithayanukul
P
P
Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand. pypph@mahidol.ac.th
Leanpolchareanchai
J
J
Bavovada
R
R
D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D017815:Elapidae; D005419:Flavonoids / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors; D050830:L-Amino Acid Oxidase / Q000037:antagonists & inhibitors; D008297:Male; D051379:Mice; D009336:Necrosis / Q000188:drug therapy / Q000517:prevention & control; D010636:Phenols / Q000494:pharmacology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012909:Snake Bites / Q000188:drug therapy; D012910:Snake Venoms / Q000009:adverse effects* / Q000201:enzymology; D013662:Tea / Q000737:chemistry*; D017819:Viperidae
D000997:Antivenins; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D011480:Protease Inhibitors; D012910:Snake Venoms; D013662:Tea; D050830:L-Amino Acid Oxidase; D010741:Phospholipases A; D006821:Hyaluronoglucosaminidase