title: Abnormality of synaptic vesicular associated proteins in cerebral cortex and hippocampus after microwave exposure.
abstract: Studies were performed to determine the effects of microwave on synaptic vesicles and the expression of synaptic vesicular associated proteins including synapsin I, VAMP-2, syntaxin, and synaptophysin. 25 Wistar rats were exposed to microwave which the average power density was 30 mW/cm(2), and whole body average specific absorption rate was 14.1 W/kg for 5 min. Synaptosome preparations in the cerebral cortex and hippocampus were obtained by isotonic Percoll/sucrose discontinuous gradients at 6 h, 1, 3, and 7 days after radiation. The expression of synaptic vesicular associated proteins was measured using Western blots and image analysis. The interaction between VAMP-2 and syntaxin was examined by coimmunoprecipitation analysis. Synapsin I in the cerebral cortex were decreased at 3 days (P < 0.01) after radiation and in the hippocampus increased at 1 day (P < 0.01), decreased at 3 days (P < 0.01), increased again at 7 days (P < 0.01) after exposure, compared with the sham-treated controls. Synaptophysin were increased in 1-7 days (P < 0.01) after exposure in the cerebral cortex and hippocampus. VAMP-2 were decreased at 1 and 3 days (P < 0.01) and syntaxin were decreased in 6 h to 3 days (P < 0.01) after radiation in the cerebral cortex and hippocampus. The interactions between VAMP-2 and syntaxin were decreased at 3-7 days (P < 0.01) after radiation in the cerebral cortex and hippocampus, compared with the sham-treated controls. These results suggest that 30 mW/cm(2) (SAR 14.1 W/kg) microwave radiation can result in the perturbation of the synaptic vesicles associated proteins: synapsin I, synaptophysin, VAMP-2, and syntaxin. The perturbation could induce the deposit of synaptic vesicle, which might be relative to the dysfunction of the synaptic transmission, even the cognition deficit.
authors: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, People's Republic of China.
authors: Lifeng
authors: L
authors: Wang
authors: Ruiyun
authors: R
authors: Peng
authors: Xiangjun
authors: X
authors: Hu
authors: Yabing
authors: Y
authors: Gao
authors: Shuiming
authors: S
authors: Wang
authors: Li
authors: L
authors: Zhao
authors: Ji
authors: J
authors: Dong
authors: Zhentao
authors: Z
authors: Su
authors: Xinping
authors: X
authors: Xu
authors: Ronglian
authors: R
authors: Gao
authors: Cuiping
authors: C
authors: Lei
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000378:metabolism / Q000528:radiation effects*; D006624:Hippocampus / Q000378:metabolism / Q000528:radiation effects*; D047468:Immunoprecipitation; D008297:Male; D008872:Microwaves; D050765:Qa-SNARE Proteins / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D016704:Synapsins / Q000378:metabolism / Q000528:radiation effects; D013572:Synaptic Vesicles / Q000378:metabolism / Q000528:radiation effects*; D016708:Synaptophysin / Q000378:metabolism / Q000528:radiation effects; D050988:Vesicle-Associated Membrane Protein 2 / Q000378:metabolism / Q000528:radiation effects
chemical_list: D050765:Qa-SNARE Proteins; D016704:Synapsins; D016708:Synaptophysin; D050988:Vesicle-Associated Membrane Protein 2