title: Factor VIII-hydrolyzing IgG in acquired and congenital hemophilia.
abstract: Anti-factor VIII (FVIII) inhibitory IgG may arise as alloantibodies to therapeutic FVIII in patients with congenital hemophilia A, or as autoantibodies to endogenous FVIII in individuals with acquired hemophilia. We have described FVIII-hydrolyzing IgG both in hemophilia A patients with anti-FVIII IgG and in acquired hemophilia patients. Here, we compared the properties of proteolytic auto- and allo-antibodies. Rates of FVIII hydrolysis differed significantly between the two groups of antibodies. Proline-phenylalanine-arginine-methylcoumarinamide was a surrogate substrate for FVIII-hydrolyzing autoantibodies. Our data suggest that populations of proteolytic anti-FVIII IgG in acquired hemophilia patients are different from that of inhibitor-positive hemophilia A patients.
authors: Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, UMR S 872, Paris, France.
authors: Bharath
authors: B
authors: Wootla
authors: Ankit
authors: A
authors: Mahendra
authors: Jordan D
authors: JD
authors: Dimitrov
authors: Alain
authors: A
authors: Friboulet
authors: Annie
authors: A
authors: Borel-Derlon
authors: Desirazu N
authors: DN
authors: Rao
authors: Taizo
authors: T
authors: Uda
authors: Jeanne-Yvonne
authors: JY
authors: Borg
authors: Jagadeesh
authors: J
authors: Bayry
authors: Srinivas V
authors: SV
authors: Kaveri
authors: Sébastien
authors: S
authors: Lacroix-Desmazes
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017151:Antibodies, Catalytic / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D005169:Factor VIII / Q000276:immunology*; D005260:Female; D006467:Hemophilia A / Q000097:blood / Q000235:genetics / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007518:Isoantibodies / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged
chemical_list: D017151:Antibodies, Catalytic; D001323:Autoantibodies; D007074:Immunoglobulin G; D007518:Isoantibodies; D005169:Factor VIII