The development and evaluation of a continuous flow process for the lipase-mediated oxidation of alkenes.
We report the use of an immobilised form of Candida antarctica lipase B, Novozym((R)) 435, in a preliminary investigation into the development of a continuous flow reactor capable of performing the chemo-enzymatic oxidation of alkenes in high yield and purity, utilising the commercially available oxidant hydrogen peroxide (100 volumes). Initial investigations focussed on the lipase-mediated oxidation of 1-methylcyclohexene, with the optimised reaction conditions subsequently employed for the epoxidation of an array of aromatic and aliphatic alkenes in 97.6 to 99.5% yield and quantitative purity.
Wiles
Charlotte
C
Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK. c.wiles@chemtrix.com
Hammond
Marcus J
MJ
Watts
Paul
P