Selective labeling of tag-fused protein by tryptophan-sensitized luminescence of a terbium complex.
A Tb3+ complex with two di(2-picolyl)amine (DPA) moieties and an oligo-aspartate peptide containing a tryptophan residue have been designed as a new binding pair for use in protein labeling.
Hirayama
Tasuku
T
Graduate School of Human and Environmental Studies, Department of Structural Biology, Kyoto University, Yoshida, Sakyo-ku, Kyoto, 606-8501, Japan.
Taki
Masayasu
M
Kodan
Atsushi
A
Kato
Hiroaki
H
Yamamoto
Yukio
Y
D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D008163:Luminescent Measurements; D009942:Organometallic Compounds / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D010848:Picolinic Acids / Q000737:chemistry; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D013194:Staining and Labeling; D013379:Substrate Specificity; D013725:Terbium / Q000737:chemistry*; D014364:Tryptophan
C501847:2,2'-dipicolylamine; D000588:Amines; D009942:Organometallic Compounds; D010455:Peptides; D010848:Picolinic Acids; D011506:Proteins; D013725:Terbium; D014364:Tryptophan