title: Plasminogen activator inhibitor type-1 gene 4G/5G polymorphism in Turkish adult patients with asthma.
abstract: OBJECTIVE
  This study has been performed on asthmatic patients in the Turkish population to determine the frequency of 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene, and with the aim of examining the role of this polymorphism in asthma development.
  
  
  METHODS
  Genomic DNA obtained from 165 persons (98 patients with asthma and 67 healthy controls) was used in the study. DNA was multiplied with polymerase chain reaction using 4G and 5G allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. Results were evaluated with chi-square test.
  
  
  RESULTS
  No statistically significant difference between the groups with respect to genotype distribution was found (p > 0.05) in the study. The 4G allele frequency was indicated as 48% and 5G allele was as 52% in patients, whereas this was 50-50% in the control group.
  
  
  CONCLUSIONS
  It has been established by this study that 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene do not play a role in the development of asthma in the Turkish population.
authors: Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey. dcosan@ogu.edu.tr
authors: Didem
authors: D
authors: Cosan
authors: Emel
authors: E
authors: Kurt
authors: Hulyam
authors: H
authors: Kurt
authors: Irfan
authors: I
authors: Degirmenci
authors: Banu
authors: B
authors: Kuçukarabaci
authors: Muzaffer
authors: M
authors: Metintas
authors: Meral Urhan
authors: MU
authors: Kucuk
authors: Hasan Veysi
authors: HV
authors: Gunes
authors: Ertugrul
authors: E
authors: Colak
mesh_terms: D000328:Adult; D000483:Alleles; D001249:Asthma / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014421:Turkey / Q000453:epidemiology
chemical_list: D017395:Plasminogen Activator Inhibitor 1