Cadherin and integrin regulation of epithelial cell migration.
These studies quantified the relative effects of E-cadherin expression and homophilic ligation on the integrin-mediated motility of epithelial cells. Micropatterned proteins were used to quantitatively titrate the ligation of E-cadherin and integrin receptors in order to assess their coordinate influence on the migration velocities of MDA-MB-231 breast tumor epithelial cells. Fibronectin, E-cadherin, and mixtures of fibronectin and E-cadherin were covalently patterned on solid surfaces at defined compositions and mass coverages. The migration velocities of parental epithelial cells and of cells engineered to express E-cadherin under tetracycline control show that E-cadherin expression reduces cell motility by both adhesion-dependent and adhesion-independent mechanisms. Increasing E-cadherin expression levels also suppresses the dependence of cell velocity on the fibronectin coverage. On E-cadherin-containing substrata, the cell velocity decreases both with the E-cadherin expression level and with the immobilized E-cadherin surface density. These studies thus identified conditions under which E-cadherin preferentially suppresses cell migration by adhesion-independent versus adhesion-dependent mechanisms.
Silvestre
Jonathan
J
Department of Chemical & Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Kenis
Paul J A
PJ
Leckband
Deborah E
DE
D015820:Cadherins / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016023:Integrins / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D015820:Cadherins; D005353:Fibronectins; D016023:Integrins; D011506:Proteins