The analysis of core and symbiotic genes of rhizobia nodulating Vicia from different continents reveals their common phylogenetic origin and suggests the distribution of Rhizobium leguminosarum strains together with Vicia seeds.
In this work, we analysed the core and symbiotic genes of rhizobial strains isolated from Vicia sativa in three soils from the Northwest of Spain, and compared them with other Vicia endosymbionts isolated in other geographical locations. The analysis of rrs, recA and atpD genes and 16S-23S rRNA intergenic spacer showed that the Spanish strains nodulating V. sativa are phylogenetically close to those isolated from V. sativa and V. faba in different European, American and Asian countries forming a group related to Rhizobium leguminosarum. The analysis of the nodC gene of strains nodulating V. sativa and V. faba in different continents showed they belong to a phylogenetically compact group indicating that these legumes are restrictive hosts. The results of the nodC gene analysis allow the delineation of the biovar viciae showing a common phylogenetic origin of V. sativa and V. faba endosymbionts in several continents. Since these two legume species are indigenous from Europe, our results suggest a world distribution of strains from R. leguminosarum together with the V. sativa and V. faba seeds and a close coevolution among chromosome, symbiotic genes and legume host in this Rhizobium-Vicia symbiosis.
Alvarez-Martínez
Estela R
ER
Departamento de Microbiología y Genética, Lab. 209, Edificio Departamental de Biología, Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
Valverde
Angel
A
Ramírez-Bahena
Martha Helena
MH
García-Fraile
Paula
P
Tejedor
Carmen
C
Mateos
Pedro F
PF
Santillana
Nery
N
Zúñiga
Doris
D
Peix
Alvaro
A
Velázquez
Encarna
E
D001426:Bacterial Proteins / Q000235:genetics; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D019143:Evolution, Molecular; D005843:Geography; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016961:Rhizobium leguminosarum / Q000145:classification / Q000235:genetics*; D053204:Root Nodules, Plant / Q000382:microbiology; D012639:Seeds / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013030:Spain; D013559:Symbiosis / Q000235:genetics*; D031306:Vicia / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales