title: Detection of low-abundant novel transcripts in mouse hematopoietic stem cells.
abstract: Gene expression profiles of hematopoietic stem cells (HSCs) provide clues for understanding molecular mechanisms of HSC behavior, including self-renewal and differentiation. We took advantage of serial analysis of gene expression (SAGE) to identify medium- and low-abundant transcripts expressed in HSCs/hematopoietic progenitor cells (HPCs). Among a total of 31,380 unique transcripts, 17,326 (55%) correspond to known genes and, 14,054 (45%) are low-copy transcripts that have no matches to currently known genes. Among the former class, 3,899 (23%) were alternatively spliced transcripts and 3,754 (22%) represent anti-sense transcripts from known genes. Mapping of the SAGE tags to the mouse genome showed that differences in gene expression exist among chromosomes. In addition, comparison of the HSCs/HPCs SAGE data to that of myeloid progenitor cells revealed that massive genetic reprogramming occurs in hematopoietic cell differentiation. Our results demonstrate a previously unrecognized complexity of gene expression in HSCs/HPCs, and indicate the need for further efforts to fully identify and characterize the transcripts expressed in this cell type.
authors: School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
authors: Hyeng-Soo
authors: HS
authors: Kim
authors: Junmo
authors: J
authors: Hwang
authors: Young-Hun
authors: YH
authors: Kim
authors: Seonggon
authors: S
authors: Kim
authors: Jae-Woong
authors: JW
authors: Lee
authors: Hyung-Sik
authors: HS
authors: Kang
authors: Kil Soo
authors: KS
authors: Kim
authors: Ji-Hong
authors: JH
authors: Ha
authors: Jin Woong
authors: JW
authors: Chung
authors: Kyu-Tae
authors: KT
authors: Chang
authors: Zae Young
authors: ZY
authors: Ryoo
authors: Sanggyu
authors: S
authors: Lee
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D016678:Genome; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D023461:Myeloid Progenitor Cells / Q000378:metabolism; D016372:RNA, Antisense / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D016372:RNA, Antisense; D012333:RNA, Messenger