title: Bilateral high frequency stimulation of the subthalamic nucleus normalizes COX activity in the substantia nigra of Parkinsonian rats.
abstract: Part of the mechanism underlying the therapeutic effect of subthalamic nucleus (STN) high frequency stimulation (HFS) involves the activity of the basal ganglia output structures. The general idea is that STN's burst activity leads to an increased activity of the basal ganglia output nuclei and that HFS reverses this. However, the published data sets conflict. Here, we addressed the question which effect STN HFS had on the overall substantia nigra pars reticulata (SNr; one of the basal ganglia output nuclei) activity, in rats rendered Parkinsonian by 6-hydroxydopamine (6-OHDA) injections in the striatum. We used a marker for metabolic activity, cytochrome C oxidase (COX). The expression of COX in the SNr of Parkinsonian rats was significantly higher as compared to sham-operated rats. However, in Parkinsonian rats with STN HFS, expression of COX was significantly lower as compared to non-stimulated Parkinsonian rats, and was comparable to the level of expression in sham-operated rats. These results show that STN HFS decreased the overall activity of the basal ganglia output nucleus in dopamine-depleted rats.
authors: Department of Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, Maastricht, The Netherlands.
authors: Rinske
authors: R
authors: Vlamings
authors: Veerle
authors: V
authors: Visser-Vandewalle
authors: Ramazan
authors: R
authors: Kozan
authors: Suleyman
authors: S
authors: Kaplan
authors: Harry W M
authors: HW
authors: Steinbusch
authors: Yasin
authors: Y
authors: Temel
mesh_terms: D000818:Animals; D002452:Cell Count; D003342:Corpus Striatum / Q000187:drug effects; D046690:Deep Brain Stimulation; D004298:Dopamine / Q000378:metabolism; D004567:Electrodes, Implanted; D003576:Electron Transport Complex IV / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000201:enzymology*; D016627:Oxidopamine / Q000633:toxicity; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000201:enzymology*; D051381:Rats; D011917:Rats, Inbred Lew; D013378:Substantia Nigra / Q000201:enzymology*; D020531:Subthalamic Nucleus / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D003576:Electron Transport Complex IV; D004298:Dopamine