Effect of cocultivation of brain tissues on leucine and glycerol-3-phosphate incorporation.
Studies on the interaction between cortex grey matter (CC), diencephalon (DE), mesencephalon (ME) and pure glial cells free of neurons were undertaken in explant cultures. The culture system provided the formation of neuritic bridges between individual tissue pieces. Cocultivation of different brain tissues resulted in changes of leucine and glycerol-3-phosphate incorporation characteristic for individual brain areas. Glial cell (GC) did not respond to innervation by neurites of various sources. Glycerol-3-phosphate uptake rates were substantially altered in cocultures containing neurons and glia.
Giesing
M
M
Institute for Physiological Chemistry, Laboratory for Nerve Tissue Culture, Nussallee 11, 5300 Bonn G.F.R.
Sottong
U
U
Gerken
U
U
Zilliken
F
F