Androgen receptor and 17beta-HSD type 2 regulation in neonatal mouse lung development.
A QPCR analysis of androgen receptor and several androgen metabolizing genes was performed during the saccular and alveolar stages of mouse lung development. Androgen receptor expression showed a statistically significant increase during the alveolar stage while levels of 17beta-hydroxysteroid dehydrogenase type 2 (17beta-HSD 2) expression significantly decreased at the end of the saccular stage and remained low throughout the alveolar period. 17beta-HSD 1, 17beta-HSD 5, 5alpha-reductase type 1, and mouse 3alpha-HSD did not present such a regulation. The androgen receptor protein was primarily detected in the nucleus of airway epithelial cells and of a subset of respiratory epithelial cells. 17beta-HSD 2 mRNA co-localized with androgen receptor protein during saccularization, but was absent from airway epithelium during alveolarization. Taken together, our results demonstrate temporal and spatial regulation of androgen receptor and 17beta-HSD 2 during the sacculo-alveolar transition period of mouse lung development suggesting control of androgen action.
Boucher
Eric
E
Laboratory of Ontogeny and Reproduction, Centre Hospitalier Universitaire de Québec (CHUQ), Pavillon Centre Hospitalier de l'Université Laval (CHUL), Québec City, Québec, Canada.
Provost
Pierre R
PR
Plante
Julie
J
Tremblay
Yves
Y
D015067:17-Hydroxysteroid Dehydrogenases; D000818:Animals; D000831:Animals, Newborn; D004960:Estradiol Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008168:Lung / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development*; D051379:Mice; D016133:Polymerase Chain Reaction; D011650:Pulmonary Alveoli / Q000201:enzymology / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D011944:Receptors, Androgen; D015067:17-Hydroxysteroid Dehydrogenases; C032690:3-alpha-(17-beta)-hydroxysteroid dehydrogenase (NAD(+)); D004960:Estradiol Dehydrogenases