Lutaonella thermophila gen. nov., sp. nov., a moderately thermophilic member of the family Flavobacteriaceae isolated from a coastal hot spring.
A yellow-pigmented, Gram-staining-negative, aerobic, non-motile, moderately thermophilic, rod-shaped bacterium, CC-MHSW-2T, was isolated from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. The 16S rRNA gene sequence analysis demonstrated that it shared <93.2% sequence similarity with Aquimarina species. The organism was unable to produce acid from carbohydrates, but it could utilize a number of organic acids and amino acids. Menaquinone 6 (MK-6) was the major respiratory quinone and iso-C15:0, iso-C17:0 3-OH and summed feature 3 (comprising C16:1omega7c and/or C16:1omega6c) were the predominant fatty acids. This fatty acid profile distinguished the isolate from members of the genera Aquimarina, Tamlana, Zhouia, Leeuwenhoekiella and Cellulophaga. The DNA G+C content of strain CC-MHSW-2T was 39.7+/-1 mol%. On the basis of the 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain CC-MHSW-2T represents a novel genus and species in the family Flavobacteriaceae, for which the name Lutaonella thermophila gen. nov., sp. nov. is proposed. The type strain is CC-MHSW-2T (=KCTC 22538T=JCM 15069T).
Arun
A B
AB
Yenepoya Research Center, Yenepoya University, University Rd, Deralakatee, Mangalore, Karnataka state, India.
Chen
Wen-Ming
WM
Lai
Wei-An
WA
Chou
Jui-Hsing
JH
Shen
Fo-Ting
FT
Rekha
P D
PD
Young
Chiu-Chung
CC
D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D008124:Locomotion; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan
D000596:Amino Acids; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S