Cytogenetic survey of species of two distinct genera of Iberian nases (Cyprinidae, Leuciscinae) that hybridize extensively in nature. I. Evidence of a similar and conserved chromosome pattern with some few species-specific markers at macro-structural level.
Pseudochondrostoma duriense and Pseudochondrostoma polylepis hybridize extensively with Achondrostoma oligolepis in natural populations. In this first survey, karyotypes were comparatively analyzed by C-, AgNOR- and CMA(3)-banding procedures in pure (non-introgressed) fish specimens. Leuciscinae pattern was evidenced in the three species: metacentrics and submetacentrics dominance, a big subtelo/acrocentric (marker) chromosome pair and a 2n = 50; small macro-structural differences were observed. Heterochromatin was centromere-associated. Exceptions were found at sm1 and st/a1 long arms and at m1, sm3 and sm6 short arms. The st/a1 band was telomeric in the straight-mouth nases and sub-terminal in A. oligolepis. Multiple NORs of heterochromatic nature were found in sm pairs of the three species. Signals were telomeric except for one pair in A. oligolepis. Two to four structural and two functional NORs were found in P. duriense and P. polylepis, and four to six structural and four functional NORs in A. oligolepis. Species-specific markers will prove useful in hybrid zones' cytogenetic characterization and for in-depth studies of genome compatibility-related issues in future studies.
Pereira
Carla
C
Universidade de Lisboa, Faculdade de Ciências, Departamento de Biologia Animal/Centro de Biologia Ambiental, Campo Grande, C2, 3 masculine Piso, 1749-016, Lisbon, Portugal.
Neto
Américo
A
Collares-Pereira
Maria João
MJ
D000818:Animals; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D003433:Crosses, Genetic; D003530:Cyprinidae / Q000235:genetics* / Q000502:physiology; D020732:Cytogenetic Analysis; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation / Q000502:physiology; D006824:Hybridization, Genetic; D019368:Nature; D013045:Species Specificity
D005819:Genetic Markers