title: PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts.
abstract: We isolate and culture carcinoma-associated fibroblasts (CAFs) from primary tumour (CAFpt), CAFs from corresponding synchronous liver metastasis (CAFlm) as well as normal colonic fibroblasts (NCF) from the same patient. From these cultures, conditioned media (CM) was obtained. Culture of a wide panel of colorectal and pancreatic cell lines in CM from CAFlm resulted in overexpression of mRNA PRL-3 and higher overexpression in CAFs than in non-activated fibroblasts. Moreover PRL-3 mRNA expression correlates with expression of alpha-SMA and deposition of collagen fibrils in the stroma. We demonstrate that products secreted by CAFs trigger PRL-3 overexpression in cancer cells. Identification of these factors may contribute to new stroma-targeted therapies for desmoplastic tumours.
authors: Translational Research Laboratory, Institut Català d'Oncologia, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), 08907 L'Hospitalet de Llobregat, Barcelona, Spain. dgmollevi@iconcologia.net
authors: David G
authors: DG
authors: Molleví
authors: Alvaro
authors: A
authors: Aytes
authors: Mireia
authors: M
authors: Berdiel
authors: Laura
authors: L
authors: Padullés
authors: Maria
authors: M
authors: Martínez-Iniesta
authors: Xavier
authors: X
authors: Sanjuan
authors: Ramon
authors: R
authors: Salazar
authors: Alberto
authors: A
authors: Villanueva
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D016015:Logistic Models; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000235:genetics; D017154:Stromal Cells / Q000201:enzymology
chemical_list: D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases