title: Evaluation of encapsulated Newcastle disease virus liposomes using various phospholipids administered to improve chicken humoral immunity.
abstract: We propose the adjuvant effects of phospholipid liposome compositions using intranasal inoculation of a liposomal-Newcastle disease virus (NDV) vaccine in chickens. The immunogenicity of three liposome formulations was determined in chickens using the hemagglutination-inhibition (HI) test, nasal secretory immunoglobulin A and serum immunoglobulin A (IgG) antibody titers using the enzyme-linked immunosorbent assay. The immune response against NDV antigens was determined after immunization with neutral charged liposomes composed of egg phosphatidylcholine (EPC) (60 micromol), cholesterol (Chol) (15 micromol), and EPC-liposomes (EPC-Lip), which elicited strong systemic (serum) and local (nasal) humoral responses. However, the intranasal administration with cationic charged liposomes composed of EPC (30 micromol), stearylamine (SA) (15 micromol), Chol (15 micromol), and SA-liposomes (SA-Lip) induced poor humoral immune responses. Only the vaccine formulated with anionic charged liposomes composed of EPC (30 micromol), dipalmitoylphosphatidylserine (15 micromol), Chol (15 micromol), and phosphatidylserine-liposomes (PS-Lip) elicited the highest titers of HI antibodies. These are the first results to suggest that antigen delivery using EPC-Lip is very useful in enhancing antibody production at the mucosal site and in serum.
authors: Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: Li-Ping
authors: LP
authors: Tseng
authors: Graduate Institute of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors: Chwei-Jang
authors: CJ
authors: Chiou
authors: Department of Hog Cholera, Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan.
authors: Ming-Chung
authors: MC
authors: Deng
authors: Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan.
authors: Mei-Hsiu
authors: MH
authors: Lin
authors: Department of Disaster Management, Taiwan Police College, Taipei, Taiwan.
authors: Ryh-Nan
authors: RN
authors: Pan
authors: Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: Yi-You
authors: YY
authors: Huang
authors: Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan.
authors: Der-Zen
authors: DZ
authors: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002645:Chickens / Q000276:immunology*; D004337:Drug Carriers; D004339:Drug Compounding; D004797:Enzyme-Linked Immunosorbent Assay; D005079:Excipients; D006385:Hemagglutination Inhibition Tests; D056724:Immunity, Humoral; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D008081:Liposomes; D009522:Newcastle disease virus / Q000276:immunology*; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D014132:Trachea / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D005079:Excipients; D007074:Immunoglobulin G; D008081:Liposomes; D010743:Phospholipids; D014765:Viral Vaccines