title: CTSS promoter -25G/A: not a risk factor for CHD in Chinese.
abstract: OBJECTIVE
  Atherosclerosis is an inflammatory disease characterized by extensive remodelling of the extracellular matrix architecture of the arterial wall. Recent data suggested the participation of lysosomal cysteine proteases such as cathepsin S (CTSS) in atherogenesis. The G/A polymorphism at nucleotide -25 was reported to be located in the promoter of the CTSS gene. The aim of this study was to observe the association between CTSS -25G/A polymorphism and the risk of coronary heart disease (CHD) in a Chinese population sample.
  
  
  METHODS
  The polymerase chain reaction and restriction digestion methods were performed to screen the CTSS gene -25G/A polymorphism in a Chinese population sample with (n = 659) or without CHD (n = 352). Traditional risk factors for CHD were obtained simultaneously.
  
  
  RESULTS
  The frequencies of the G and A allele in the total population was 0.630 and 0.370, respectively. The frequencies of -25G/A polymorphism in the CTSS gene (G: 0.626 vs. 0.633, P > 0.05; A: 0.374 vs. 0.367, P > 0.05) and the genotype distribution (G: 83.4 vs. 85.3, P > 0.05; A: 58.0 vs. 58.5, P > 0.05) were similar between CHD patients and control subjects. Moreover, CTSS genotype was not associated with severity of coronary stenosis (P > 0.05) in CHD patients.
  
  
  CONCLUSIONS
  The CTSS -25G/A polymorphism was not related with CHD in our Chinese population sample.
authors: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Disease, Shanghai, China. ajsun@zshospital.net
authors: Aijun
authors: A
authors: Sun
authors: Kumar Rauniyar
authors: KR
authors: Binay
authors: Feiyu
authors: F
authors: Xiang
authors: Ji
authors: J
authors: Zhao
authors: Ying
authors: Y
authors: Wang
authors: Lei
authors: L
authors: Xu
authors: Huili
authors: H
authors: Ma
authors: Keqiang
authors: K
authors: Wang
authors: Yunzeng
authors: Y
authors: Zou
authors: Wei
authors: W
authors: Huang
authors: Junbo
authors: J
authors: Ge
mesh_terms: D016022:Case-Control Studies; D002403:Cathepsins / Q000235:genetics* / Q000378:metabolism; D002681:China / Q000453:epidemiology; D016001:Confidence Intervals; D003327:Coronary Disease / Q000201:enzymology* / Q000453:epidemiology / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D012720:Severity of Illness Index
chemical_list: D002403:Cathepsins; C028217:cathepsin S