[The construction of a preliminary genetic linkage map in the Japanese scallop Mizuhopecten yessoensis].
AFLP markers were used to construct the primary linkage map in a family of Mizuhopecten yessoensis. A total of 1 855 markers were generated in two parents and 52 progenies of the mapping family by using 56 AFLP primer combinations. Among the 1 855 markers, 598 were polymorphic and 354 were in agreement with the Mendelian segregating ratio of 1:1. Markers segregated according to Mendelian 1:1 ratio (P>0.05) and 23 distorted markers (0.01<P<0.05) were used in linkage analysis. The male map was composed of 178 markers in 22 linkage groups, covering a total of 1 263.0 cM with the average interval of 8.1 cM between markers, and the observed coverage was 79.4%. The female map was composed of 163 markers in 22 linkage groups, covering a total of 1 259.5 cM with the average interval of 8.9 cM between markers, and the observed coverage was 76.8%. AFLP markers showed an uneven distribution on the linkage group and there were a lot of markers with a distance of 0.
Liu
Wei-Dong
WD
Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. cnliu51@tom.com
Bao
Xiang-Bo
XB
Song
Wen-Tao
WT
Zhou
Zun-Chun
ZC
He
Chong-Bo
CB
Yu
Xing-Ju
XJ
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D003433:Crosses, Genetic; D004247:DNA / Q000032:analysis; D005260:Female; D008040:Genetic Linkage; D005828:Genetics, Population; D007564:Japan; D008297:Male; D049897:Pectinidae / Q000235:genetics*; D011110:Polymorphism, Genetic
D004247:DNA