title: Experimental formation of periodontal structure around titanium implants utilizing bone marrow mesenchymal stem cells: a pilot study.
abstract: Tissue engineering in the head and neck area, presents numerous advantages. One of the most remarkable advantages is that regeneration of only a small amount of tissue can be highly beneficial to the patient, particularly in the field of periodontal tissue regeneration. For decades, successful osseointegration has provided thousands of restorations that maintain normal function. With the increasing need to utilize dental implants for growing patients and enhance their function to simulate normal tooth physiology and proprioception, there appears to be an urgent need for t concept of periodontal tissue regeneration around dental implants. In the present work, 5 goats wer used for immediate implant placement post canine teeth extraction. Each goat received 2 implan fixtures; the control side received a porous hollow root-form poly (DL-Lactide-co-Glycolide) scaf around the titanium fixture, and the experimental side received the same scaffold but seeded with autogenous bone marrow-derived mesenchymal stem cells. One animal was killed 10 days postoperatively, and the others were killed after 1 month. The results showed that on th experimental side, periodontal-like tissue with newly formed bone was demonstrated both at 1 days and after 1 month, while the control specimens showed early signs of connective tissue regeneration around the titanium fixture at 10 days, but was not shown in the 1 month specimens. I can be concluded that undifferentiated mesenchymal stem cells were capable of differentiating t provide the 3 critical tissues required for periodontal tissue regeneration: cementum, bone, a periodontal ligament. This work may provide a new approach for periodontal tissue regeneration.
authors: Tissue Engineering Laboratories, Faculty of Dentistry-Alexandria University, Ibrahimeya, Egypt.
authors: Mona K
authors: MK
authors: Marei
authors: Manal M
authors: MM
authors: Saad
authors: Adham M
authors: AM
authors: El-Ashwah
authors: Rania M
authors: RM
authors: El-Backly
authors: Mohammed A
authors: MA
authors: Al-Khodary
mesh_terms: D000539:Alveolar Process / Q000473:pathology / Q000502:physiology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003481:Cuspid / Q000601:surgery; D003739:Dental Cementum / Q000473:pathology / Q000502:physiology; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D006041:Goats; D019344:Lactic Acid / Q000737:chemistry; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D010513:Periodontal Ligament / Q000473:pathology / Q000502:physiology; D010519:Periodontium / Q000473:pathology / Q000502:physiology*; D010865:Pilot Projects; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012038:Regeneration / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry; D014081:Tooth Extraction; D020390:Tooth Socket / Q000601:surgery
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003764:Dental Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite; D014025:Titanium