[A new SNP microarray technology based on single base extension].
Investigation of DNA-protein interactions is fundamental to understand the mechanism underlying a variety of life processes. In this article, various types of biochemical methods in DNA-protein interaction study in vivo and in vitro at the level of DNA, protein, and the complex, respectively were briefly reviewed. Traditional assays including Nitrocellulose filter-binding assay, Footprinting, EMSA, and Southwestern blotting were summarized. In addition, chromatin immunopre-cipitation techniques including nChIP, xChIP, and ChIP-on-chip, which were widely used in epigenetics, were particularly introduced.
Dong
Yuan-Yuan
YY
National Engineering Center for Biochip at Shanghai, Shanghai 201203, China. dongyuanyuan_dyy@yahoo.com.cn
Sheng
Hai-Hui
HH
Yang
Qian
Q
Zeng
Xian-Ting
XT
Wu
Xue-Mei
XM
D005838:Genotype; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*