title: Antibody-based screen for ionizing radiation-dependent changes in the Mammalian proteome for use in biodosimetry.
abstract: In an effort to identify proteomic changes that may be useful for radiation biodosimetry, human cells of hematological origin were treated with ionizing radiation or mock-irradiated and then harvested at different times after treatment. Protein lysates were generated from these cells and evaluated by Western blotting using a panel of 301 commercially available antibodies targeting 161 unique proteins. From this screen, we identified 55 ionizing radiation-responsive proteins, including 14 proteins not previously reported to be radiation-responsive at the protein level. The data from this large-scale screen have been assembled into a public website ( http://labs.fhcrc.org/paulovich/biodose_index.html ) that may be of value to the radiation community both as a source of putative biomarkers for biodosimetry and also as a source of validation data on commercially available antibodies that detect radiation-responsive proteins. Using a panel of candidate radiation biomarkers in human cell lines, we demonstrate the feasibility of assembling a complementary panel of radiation-responsive proteins. Furthermore, we demonstrate the feasibility of using blood cell-based proteomic changes for biodosimetry by demonstrating detection of protein changes in circulating cells after total-body irradiation in a canine model.
authors: Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors: Richard G
authors: RG
authors: Ivey
authors: Oby
authors: O
authors: Subramanian
authors: Travis D
authors: TD
authors: Lorentzen
authors: Amanda G
authors: AG
authors: Paulovich
mesh_terms: D000818:Animals; D000906:Antibodies / Q000528:radiation effects*; D015153:Blotting, Western; D002460:Cell Line; D004285:Dogs; D006801:Humans; D010766:Phosphorylation; D020543:Proteome / Q000528:radiation effects*; D011829:Radiation Dosage; D012107:Research Design
chemical_list: D000906:Antibodies; D020543:Proteome