title: Regulation of the low-dose radiation paracrine-specific anchorage-independent growth response by annexin A2.
abstract: Here we identify the release of annexin A2 into the culture medium in response to low-dose X-radiation exposure and establish functional linkages to an established paracrine factor-mediated anchorage-independent growth response. Using a standard bicameral coculture model, we demonstrate that annexin A2 is secreted into the medium by irradiated cells (seeded in upper chamber) and is capable of binding to nonirradiated neighboring cells (seeded in lower chamber). The paracrine factor-mediated anchorage-independent growth response to low-dose X irradiation is reduced when irradiated annexin A2-silenced (shRNA) JB6 cells are co-cultured with nonirradiated cells relative to co-culture with irradiated annexin A2-competent vector control cells. Consistent with this observation, purified bovine annexin A2 tetramer induces anchorage-independent growth. These observations suggest that annexin A2 regulates, in part, the radiation paracrine factor-specific anchorage-independent growth response in JB6 cells.
authors: Cell Biology and Biochemistry, Pacific Northwest National Laboratory, Richland, Washington 99354, USA. Thomas.Weber@pnl.gov
authors: Thomas J
authors: TJ
authors: Weber
authors: Lee K
authors: LK
authors: Opresko
authors: David M
authors: DM
authors: Waisman
authors: Greg J
authors: GJ
authors: Newton
authors: Ryan D
authors: RD
authors: Quesenberry
authors: Nikki
authors: N
authors: Bollinger
authors: Ronald J
authors: RJ
authors: Moore
authors: Richard D
authors: RD
authors: Smith
mesh_terms: D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D017306:Annexin A2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects*; D018920:Coculture Techniques; D005341:Fibrinolysin / Q000378:metabolism; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D019899:Paracrine Communication / Q000528:radiation effects*; D010958:Plasminogen / Q000378:metabolism; D034622:RNA Interference; D011829:Radiation Dosage
chemical_list: D017306:Annexin A2; D010958:Plasminogen; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D005341:Fibrinolysin