Variation in the safety of induced pluripotent stem cell lines.
We evaluated the teratoma-forming propensity of secondary neurospheres (SNS) generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways. Teratoma-formation of SNS from embryonic fibroblast-derived iPS cells was similar to that of SNS from embryonic stem (ES) cells. In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells.
Miura
Kyoko
K
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Okada
Yohei
Y
Aoi
Takashi
T
Okada
Aki
A
Takahashi
Kazutoshi
K
Okita
Keisuke
K
Nakagawa
Masato
M
Koyanagi
Michiyo
M
Tanabe
Koji
K
Ohnuki
Mari
M
Ogawa
Daisuke
D
Ikeda
Eiji
E
Okano
Hideyuki
H
Yamanaka
Shinya
S
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D051379:Mice; D009474:Neurons / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000166:cytology*; D012449:Safety; D013724:Teratoma / Q000473:pathology