Essential oils against foodborne pathogens and spoilage bacteria in minced meat.
The antimicrobial activity of essential oils of oregano, thyme, basil, marjoram, lemongrass, ginger, and clove was investigated in vitro by agar dilution method and minimal inhibitory concentration (MIC) determination against Gram-positive (Staphylococcus aureus and Listeria monocytogenes) and Gram-negative strains (Escherichia coli and Salmonella Enteritidis). MIC(90%) values were tested against bacterial strains inoculated experimentally in irradiated minced meat and against natural microbiota (aerobic or facultative, mesophilic, and psychrotrophic bacteria) found in minced meat samples. MIC(90%) values ranged from 0.05%v/v (lemongrass oil) to 0.46%v/v (marjoram oil) to Gram-positive bacteria and from 0.10%v/v (clove oil) to 0.56%v/v (ginger oil) to Gram-negative strains. However, the MIC(90%) assessed on minced meat inoculated experimentally with foodborne pathogen strains and against natural microbiota of meat did not show the same effectiveness, and 1.3 and 1.0 were the highest log CFU/g reduction values obtained against tested microorganisms.
Barbosa
Lidiane Nunes
LN
Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University, Brazil.
Rall
Vera Lucia Mores
VL
Fernandes
Ana Angélica Henrique
AA
Ushimaru
Priscila Ikeda
PI
da Silva Probst
Isabella
I
Fernandes
Ary
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D045783:Clove Oil / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005514:Food Irradiation; D020939:Zingiber officinale / Q000737:chemistry; D008089:Listeria monocytogenes / Q000187:drug effects; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D031339:Ocimum; D009822:Oils, Volatile / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry; D010938:Plant Oils / Q000494:pharmacology; D012477:Salmonella enteritidis / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013729:Terpenes / Q000494:pharmacology; D046930:Thymus Plant / Q000737:chemistry
D000900:Anti-Bacterial Agents; D045783:Clove Oil; D009822:Oils, Volatile; D010938:Plant Oils; D013729:Terpenes; C052901:lemongrass oil; C051168:basil oil