title: B-1 cell participation in T-cell-mediated alloimmune response.
abstract: B-1 and B cells are important producers of natural antibodies in mice and humans and, therefore, are considered as the first line of defense against pathogens. Because of that, their role in T-cell-mediated immune responses is commonly underrated. However, recent studies have described the participation of B-1 cells in immediate and delayed-type hypersensitivity. The present work assessed the role of B-1 cells in the rejection of allografts in mice, an immune reaction mainly orchestrated by T cells. We have transplanted allogeneic skin and heart to wild-type and B-1-cell-deficient mice, and followed rejection kinetics. Skin graft-infiltrating cells were analyzed by flow cytometry. We observed a delay in rejection kinetics of B-1-cell-deficient mice when compared to wild-type mice. Adoptive transfer of B-1 cells into B-1-cell-deficient mice abrogated this delay. The longer survival observed in the absence of B-1 cells correlated with less CD8+ T cells infiltrating the grafts, as well as with more mast cells. Collectively, our results show the participation of B-1 cells in the allograft rejection process in mice and collaborate to the understanding of B-1 cell biology.
authors: Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, Brazil.
authors: Mauro F
authors: MF
authors: Nogueira-Martins
authors: Mario
authors: M
authors: Mariano
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D016027:Heart Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010537:Peritoneum / Q000276:immunology; D010994:Pleura / Q000276:immunology; D035422:Pleural Cavity / Q000276:immunology; D016038:Skin Transplantation / Q000276:immunology; D019737:Transplants