[Expression of high mobility group protein A in male mouse testicular cell lines].
OBJECTIVE
To detect the expression of high mobility group protein A (HMGA) in male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts), and to pave the theoretical ground for further investigation of the action mechanism of the HMGA gene in male mouse spermatogenesis.


METHODS
We detected the expressions of HMGA1 and HMGA2 by RT-PCR and Western blot in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts).


RESULTS
HMGA1 and HMGA2 were expressed in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts) at both mRNA and protein levels. Western blot and RT-PCR methods showed similar results.


CONCLUSIONS
The expression of HMGA may be involved in the cell division and proliferation of TM4, GC-1spg and GC-2spd(ts) and play an important role in spermatogenesis of male mice.
Ma
Zheng-bing
ZB
Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, China.
Yang
Ying
Y
Wang
Jun
J
Li
Lei
L
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D049109:Cell Proliferation; D015870:Gene Expression; D025741:HMGA1a Protein / Q000235:genetics*; D025743:HMGA2 Protein / Q000235:genetics*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology* / Q000378:metabolism
D025743:HMGA2 Protein; D012333:RNA, Messenger; D025741:HMGA1a Protein