Parthenogenic activation of surplus in vitro-matured human oocytes: a tool for validation of oocyte cryopreservation.
Controlled-rate frozen and vitrified mature human oocytes were thawed or warmed and parthenogenically activated using ionomycin and 6-dimethylaminopurine to assess pronuclear development compared with fresh controls. This chemical activation model provides a useful tool for laboratories to assess proficiency before offering oocyte cryopreservation to their patients.
Morbeck
Dean E
DE
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, Minnesota 55902, USA. Morbeck.Dean@mayo.edu
Walker
David L
DL
Fredrickson
Jolene R
JR
Barud
Kathrynne M
KM
Coddington
Charles C
CC
D002470:Cell Survival; D015925:Cryopreservation / Q000592:standards*; D005260:Female; D005307:Fertilization in Vitro / Q000592:standards*; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D009865:Oocytes / Q000166:cytology*; D010312:Parthenogenesis; D015203:Reproducibility of Results