Inter- and intraspecies plasmid-mediated transfer of florfenicol resistance in Enterobacteriaceae isolates from swine.
Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for florfenicol between 4 to 128 mg/liter.
Rayamajhi
Nabin
N
Department of Infectious Disease, College of Veterinary Medicine, and KRF Priority Zoonotic Disease Research Institute, Seoul National University, San 56-1, Shilim 9 dong, Kwanak-Ku, Seoul 151-742, South Korea.
Cha
Seung Bin
SB
Kang
Mi Lan
ML
Lee
Su In
SI
Lee
Hee Soo
HS
Yoo
Han Sang
HS
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D022761:Gene Transfer, Horizontal; D010957:Plasmids; D016133:Polymerase Chain Reaction; D013552:Swine / Q000382:microbiology*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C035534:florfenicol; D013839:Thiamphenicol