DNase gamma-dependent and -independent apoptotic DNA fragmentations in Ramos Burkitt's lymphoma cell line.
DNA fragmentation is a biochemical hallmark of apoptosis. Several endonucleases, including CAD/DFF40 and endonuclease G, are implicated in DNA fragmentation. DNase gamma has also been considered to be one of the enzymes involved, but its role in relation to CAD/DFF40 in apoptosis has not been fully elucidated. Here, we distinguished between DNase gamma-dependent and CAD/DFF40-dependent DNA fragmentations. We found that DNase gamma activities appeared in the late apoptotic phase and accelerated DNA fragmentation. Thus, even if the apoptotic DNA fragmentation is initiated by CAD/DFF40, DNase gamma is required for the more complete digestion of the genomic DNA in dying cells.
Mizuta
Ryushin
R
Division of Molecular Biology, Research Institute for Biological Sciences, Tokyo University of Science, Noda, Chiba, Japan. mizuta@rs.noda.tus.ac.jp
Mizuta
Midori
M
Araki
Shinsuke
S
Suzuki
Kohei
K
Ebara
Shota
S
Furukawa
Yuki
Y
Shiokawa
Daisuke
D
Tanuma
Sei-ichi
S
Kitamura
Daisuke
D
D017209:Apoptosis / Q000502:physiology*; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D053938:DNA Fragmentation; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism; D006801:Humans; D000075223:Poly-ADP-Ribose Binding Proteins; D019311:Staurosporine / Q000378:metabolism
D004791:Enzyme Inhibitors; D000075223:Poly-ADP-Ribose Binding Proteins; C113696:DFFB protein, human; D003851:Deoxyribonucleases; D004706:Endodeoxyribonucleases; C109663:caspase-activated deoxyribonuclease; C105124:deoxyribonuclease gamma; D019311:Staurosporine