Improved protocols for the illumina genome analyzer sequencing system.
In this unit, we describe a set of improvements we have made to the standard Illumina Genome Analyzer protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data.
Quail
Michael A
MA
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom.
Swerdlow
Harold
H
Turner
Daniel J
DJ
D000818:Animals; D004247:DNA / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D016678:Genome; D006801:Humans; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods; D013698:Templates, Genetic
D004247:DNA