Capillary zone electrophoresis method for determination of bitter (alpha- and beta-) acids in hop (Humulus lupulus L.) cone extracts.
OBJECTIVE
Humulus lupulus (H. lupulus), more commonly known as hop, is a member of the Cannabaceae family with male and female flowers on separate plants. It is native in Europe including Lithuania, Asia and North America. Hop has been recognized as a medicinal plant for centuries, nevertheless different medicinal activities of hop are currently investigated and discovered. An important class of hop compounds is the hop acids, which are classified as alpha-acids and beta-acids. Different varieties of hops vary in amount and composition of hop acids.


METHODS
Simple capillary zone electrophoresis method has been optimized and applied for the analysis of hop acids in hop cone extracts.


RESULTS
With this method the analysis takes ca. 10 min. Repeatability for migration times and peak areas expressed as relative standard deviation were up to 0.21% and 5.96%, respectively.


CONCLUSIONS
Comparative results of capillary zone electrophoretic analysis of extracts of different hop varieties and conductometric titration, as a standard method for determination of alpha-acids, are presented. Both methods provide consistent results, however capillary zone electrophoresis is capable of separating co- form of humulones from other forms.
Kornysova
O
O
Department of Biochemistry and Biotechnologies, Vytautas Magnus University, Kaunas, Lithuania.
Stanius
Z
Z
Obelevicius
K
K
Ragazinskiene
O
O
Skrzydlewska
E
E
Maruska
A
A
D053138:Cyclohexenes / Q000032:analysis* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D035264:Flowers / Q000737:chemistry*; D027582:Humulus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013729:Terpenes / Q000032:analysis* / Q000302:isolation & purification
D053138:Cyclohexenes; D010936:Plant Extracts; D013729:Terpenes; C008299:lupulon; C007966:humulon