Real-time quantitative reverse transcription-PCR assays specifically detecting bluetongue virus serotypes 1, 6, and 8.
Bluetongue virus (BTV) is a major pathogen of ruminants. Especially serotypes 1, 6, and 8 are of concern to veterinary authorities in central Europe. This article describes highly sensitive real-time reverse transcription-PCR assays directed to BTV genome segment 2 for specific detection of BTV-1, -6, or -8 in animal samples.
Hoffmann
Bernd
B
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riems, Germany.
Eschbaumer
Michael
M
Beer
Martin
M
D000818:Animals; D001819:Bluetongue / Q000175:diagnosis* / Q000821:virology*; D001820:Bluetongue virus / Q000145:classification* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005060:Europe; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012418:Ruminants; D012680:Sensitivity and Specificity
D017931:DNA Primers