Dietary catechol causes increased oxidative DNA damage in the livers of mice treated with acetaminophen.
We have shown that direct reaction of catechol with nitric oxide (NO) results in generation of reactive oxygen and nitrogen species (RNS) through semiquinone radical formation, leading to oxidative DNA damage in rat forestomach. In the present study, we investigated whether dietary catechol systemically exerts the same effects under NO-rich circumstances, when given before and during induction of inflammatory lesions. Male ICR mice were treated with or without 0.8% catechol in the diet for 2 weeks followed by acetaminophen (APAP) administration at a dose of 300mg/kg by single i.p. injection. Along with several indicators of APAP-induced hepatitis, 8-hydroxydeoxyguanosine (8-OHdG) levels and immunohistochemistry for 3-nitrotyrosine (NO(2)Tyr) in the livers were examined at 1.5, 4 and 24h after APAP injection. 8-OHdG was significantly increased at 24h in the co-treatment group, but not with either catechol or APAP alone. Elevation of serum ALT and AST activities, decrease of reduced glutathione levels and histopathological liver changes were observed to the same extents in both APAP-treated groups. In view of the finding of positive hepatocytes for NO(2)Tyr prior to generation of 8-OHdG, the process of oxidative DNA damage might involve RNS formation. Precise quantitative analysis of NO(2)Tyr by means of liquid chromatography with tandem mass spectrometry (LC-MS/MS) in an additional study with the same experimental protocol confirmed increase of RNS due to the reaction of catechol with NO produced after APAP-induced hepatitis. The overall data imply that antioxidants with a catechol structure can cause oxidative DNA damage under inflammatory conditions.
Ishii
Yuji
Y
Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. y-ishii@nihs.go.jp
Okamura
Toshiya
T
Inoue
Tomoki
T
Tasaki
Masako
M
Umemura
Takashi
T
Nishikawa
Akiyoshi
A
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000082:Acetaminophen / Q000633:toxicity*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004032:Diet; D005978:Glutathione / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D013997:Time Factors; D000637:Transaminases / Q000097:blood; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D002396:Catechols; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D000082:Acetaminophen; D014443:Tyrosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000637:Transaminases; D000410:Alanine Transaminase; D003849:Deoxyguanosine; D005978:Glutathione; C034221:catechol