title: Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages.
abstract: OBJECTIVE
  The root of Scutellaria baicalensis Georgi (Labiatae), also known as Scutellariae Radix, possesses anticancer, antiviral, and anti-inflammatory properties. And it is one of the most widespread herbal remedies used in Oriental medicine. In the present study, we investigated the effects of Scutellariae Radix water extract (SR) on proinflammatory mediators secreted from lipopolysaccharide (LPS)-induced RAW 264.7 macrophages.
  
  
  METHODS
  Cell viability was assessed by MTT assay and nitric oxide (NO) concentration in the cultured medium was determined by the Griess reaction. Various Cytokines released from LPS-induced Raw 264.7 cells were measured in the cell culture supernatants using a multiplex bead array assay based on xMAP technology.
  
  
  RESULTS
  We found that SR significantly inhibited the production of NO, interleukin (IL)-3, IL-6, IL-10, IL-12p40, IL-17, interferon-inducible protein (IP)-10, keratinocyte-derived chemokine (KC), and vascular endothelial growth factor (VEGF) in LPS-induced RAW 264.7 cells at the concentrations of 25, 50, 100, 200 microg/ml (p<0.05).
  
  
  CONCLUSIONS
  These results suggest that SR has anti-inflammatory activity related with its inhibition of NO, cytokine, chemokine, and growth factor production in macrophages.
authors: Dept. of Herbology, College of Oriental Medicine, Kyungwon University, Seongnam 461-701, Republic of Korea.
authors: Seok-Bin
authors: SB
authors: Yoon
authors: Young-Jong
authors: YJ
authors: Lee
authors: Seong Kyu
authors: SK
authors: Park
authors: Ho-Cheol
authors: HC
authors: Kim
authors: Hyunsu
authors: H
authors: Bae
authors: Hyung Min
authors: HM
authors: Kim
authors: Seong-Gyu
authors: SG
authors: Ko
authors: Ho Young
authors: HY
authors: Choi
authors: Myung Sook
authors: MS
authors: Oh
authors: Wansu
authors: W
authors: Park
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D031364:Scutellaria baicalensis; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide