Analysis of glucose-dependent insulinotropic peptide receptor (GIPR) and luteinizing hormone receptor (LHCGR) expression in human adrenocortical hyperplasia.
OBJECTIVE
To analyze the aberrant expression of the GIPR and LHCGR in different forms of adrenocortical hyperplasia: ACTH-independent macronodular adrenal hyperplasia (AIMAH), primary pigmented nodular adrenocortical disease (PPNAD) and diffuse adrenal hyperplasia secondary to Cushing's disease (DAHCD).


METHODS
We quantified GIPR and LHCGR expressions using real time PCR in 20 patients with adrenocortical hyperplasia (seven with AIMAH, five with PPNAD, and eight with DAHCD). Normal adrenals tissues were used as control and the relative expression was compared with beta-actin.


RESULTS
GIPR and LHCGR expressions were demonstrated in all tissues studied. Median GIPR and LHCGR mRNA levels were 1.6; 0.4; 0.5 and 1.3; 0.9; 1.0 in adrenocortical tissues from AIMAH, PPNAD and DAHCD respectively. There were no differences between GIPR and LHCGR expressions in all tissues studied.


CONCLUSIONS
GIPR and LHCGR overexpression were not identified in the studied cases, thus suggesting that this molecular mechanism is not involved in adrenocortical hyperplasia in our patients.
Costa
Marcia Helena Soares
MH
Unidade de Endocrinologia do Desenvolvimento, Laboratório de Hormônios e Genética Molecular LIM/42, Divisão de Endocrinologia e Metabologia, São Paulo, SP, Brazil. mhsc@usp.br
Domenice
Sorahia
S
Latronico
Ana Claudia
AC
Martin
Regina Matsunaga
RM
Nishi
Mirian Yumie
MY
Lucon
Antonio Marmo
AM
Mendonca
Berenice Bilharinho
BB
Fragoso
Maria Candida Barisson Villares
MC
D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000303:Adrenal Cortex Diseases / Q000235:genetics / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D047748:Pituitary ACTH Hypersecretion / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011964:Receptors, Gastrointestinal Hormone / Q000235:genetics / Q000378:metabolism*; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D000199:Actins; D012333:RNA, Messenger; D011964:Receptors, Gastrointestinal Hormone; D011974:Receptors, LH; C041086:gastric inhibitory polypeptide receptor