Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus.
Two anti-idiotypic monoclonal antibodies (mAb2s; named 94-2 and 94-7), were generated from a BALB/c mouse immunized with human monoclonal anti-hepatitis A virus (HAV) neutralizing antibody KF94. We characterized the properties of the mAb2s and determined interactions between mAb2s, KF94 and HAV using enzyme-linked immunosorbent assay, immunofluorescence assay and HAV infectivity assay. Inactivated HAV inhibited mAb2 binding to KF94, indicating that the mAb2s mimicked the HAV neutralization site that was complementary to the paratope of KF94. MAb2 94-7 competed with an anti-HAV cellular receptor antibody for binding to HAV-susceptible cells and partially blocked virus infection. We speculated that mAb2 94-7 mimicked a portion of the HAV receptor-binding site. The ability to generate mAb2 implies that HAV receptor-binding sites are exposed on the surface of HAV, permitting antibody access.
Kiyohara
Tomoko
T
Department of Virology II, National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan. kiyohara@nih.go.jp
Totsuka
Atsuko
A
Yoneyama
Tetsuo
T
Ishii
Koji
K
Ito
Toshihiro
T
Wakita
Takaji
T
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D001665:Binding Sites / Q000276:immunology; D001667:Binding, Competitive; D002460:Cell Line; D035922:Hepatitis A Antibodies / Q000276:immunology* / Q000378:metabolism; D030041:Hepatitis A virus / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D009500:Neutralization Tests; D011991:Receptors, Virus / Q000276:immunology* / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D035922:Hepatitis A Antibodies; D011991:Receptors, Virus