title: Colorimetric protein sensing by controlled assembly of gold nanoparticles functionalized with synthetic receptors.
abstract: A novel strategy is described for the colorimetric sensing of proteins, based on polypeptide-functionalized gold nanoparticles. Recognition is accomplished using a polypeptide sensor scaffold designed to specifically bind to the model analyte, human carbonic anhydrase II (HCAII). The extent of particle aggregation, induced by the Zn(2+)-triggered dimerization and folding of a second polypeptide also present on the surface of the gold nanoparticle, gives a readily detectable colorimetric shift that is dependent on the concentration of the target protein. In the absence of HCAII, particle aggregation results in a major redshift of the plasmon peak, whereas analyte binding prevented the formation of dense aggregates, significantly reducing the magnitude of the redshift. The versatility of the technique is demonstrated using a second model system based on the recognition of a peptide sequence from the tobacco mosaic virus coat protein (TMVP) by a recombinant antibody fragment (Fab57P). Concentrations down to approximately 10 nM and approximately 25 nM are detected for HCAII and Fab57P, respectively. This strategy is proposed as a generic platform for robust and specific protein analysis that can be further developed to monitor a wide range of target proteins.
authors: Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83 Linköping, Sweden.
authors: Daniel
authors: D
authors: Aili
authors: Robert
authors: R
authors: Selegård
authors: Lars
authors: L
authors: Baltzer
authors: Karin
authors: K
authors: Enander
authors: Bo
authors: B
authors: Liedberg
mesh_terms: D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning
chemical_list: D006046:Gold