X-ray photoelectron spectroscopy- and surface plasmon resonance-detected photo release of photolabile protecting groups from nucleoside self-assembled monolayers on gold surfaces.
The formation of self-assembled monolayers (SAMs) on gold by 2-(5-iodo-2-nitrophenyl) propoxycarbonyl (I-NPPOC)-protected thymidine with an attached mercaptohexyl succinate linker and the kinetics of photochemical release of the I-NPPOC group were monitored using X-ray photoelectron spectroscopy (XPS) and surface plasmon resonance (SPR) detection. In the XPS spectra, the iodine peaks allowed for specific and accurate monitoring of the presence and loss of I-NPPOC groups on the surface. In the SPR experiment, the overall signal change on photoillumination is in accord with a theoretical estimation of the density of I-NPPOC groups in a dense monolayer. The kinetics roughly follow a biexponential time dependence with two very different time constants, corresponding to photochemical quantum yields of 0.22 and 0.0032, respectively.
Drexler
Katja
K
Department of Chemistry, University of Konstanz, Konstanz, Germany.
Smirnova
Julia
J
Galetskaya
Marina
M
Voss
Sönke
S
Fonin
Mikhail
M
Boneberg
Johannes
J
Rüdiger
Ulrich
U
Leiderer
Paul
P
Steiner
Ulrich E
UE
D000327:Adsorption; D004583:Electrons; D006046:Gold / Q000737:chemistry*; D007455:Iodine / Q000737:chemistry; D007700:Kinetics; D015394:Molecular Structure; D010777:Photochemistry; D013057:Spectrum Analysis; D019802:Succinic Acid / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013871:Thiones / Q000737:chemistry; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry*; D014965:X-Rays
D013871:Thiones; D006046:Gold; D007455:Iodine; D019802:Succinic Acid; D013936:Thymidine