title: 13C-sucrose breath test: novel use of a noninvasive biomarker of environmental gut health.
abstract: OBJECTIVE
  Environmental enteropathy syndrome may compromise growth and predispose to infectious diseases in children in the developing world, including Australian Aboriginal children from remote communities of the Northern Territory. In this study, we described the use of a biomarker (13)C-sucrose breath test (SBT) to measure enterocyte sucrase activity as a marker of small intestinal villus integrity and function.
  
  
  METHODS
  This was a hospital-based prospective case-control study of Aboriginal and non-Aboriginal children with and without acute diarrheal disease. Using the SBT, we compared 36 Aboriginal case subjects admitted to a hospital (18 diarrheal and 18 nondiarrheal disease), with 7 healthy non-Aboriginal control subjects. Intestinal permeability using the lactulose/rhamnose (L/R) ratio on a timed 90-minute blood test was performed simultaneously with the SBT. The SBT results are expressed as a cumulative percentage of the dose recovered at 90 minutes.
  
  
  RESULTS
  Aboriginal children with acute diarrheal disease have a significantly decreased absorptive capacity, as determined by the SBT, with a mean of 1.9% compared with either Aboriginal children without diarrhea (4.1%) or non-Aboriginal (6.1%) control subjects. The mean L/R ratio in the diarrhea group was 31.8 compared with 11.4 in Aboriginal children without diarrhea. There was a significant inverse correlation between the SBT and the L/R ratio.
  
  
  CONCLUSIONS
  The SBT was able to discriminate among Aboriginal children with diarrhea, asymptomatic Aboriginal children with an underlying environmental enteropathy, and healthy non-Aboriginal controls. This test provides a noninvasive, easy-to-use, integrated marker of the absorptive capacity and integrity of the small intestine and could be a valuable tool in evaluating the efficacy of interventions aimed at improving gut health.
authors: aInfectious Diseases Unit, Royal Adelaide Hospital, Adelaide, South Australia, Australia.
authors: Brett K
authors: BK
authors: Ritchie
authors: David R
authors: DR
authors: Brewster
authors: Geoffrey P
authors: GP
authors: Davidson
authors: Cuong D
authors: CD
authors: Tran
authors: Yvette
authors: Y
authors: McNeil
authors: Joanna S
authors: JS
authors: Hawkes
authors: Ross N
authors: RN
authors: Butler
mesh_terms: D001944:Breath Tests / Q000379:methods*; D002250:Carbon Radioisotopes; D002463:Cell Membrane Permeability / Q000502:physiology*; D002675:Child, Preschool; D003681:Dehydration / Q000175:diagnosis / Q000503:physiopathology; D003937:Diagnosis, Differential; D003968:Diarrhea, Infantile / Q000209:etiology* / Q000503:physiopathology; D018876:Environmental Illness / Q000175:diagnosis* / Q000503:physiopathology; D005260:Female; D006801:Humans; D007223:Infant; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000503:physiopathology; D007421:Intestine, Small / Q000503:physiopathology; D007792:Lactulose / Q000097:blood; D008297:Male; D015876:Northern Territory; D011237:Predictive Value of Tests; D012016:Reference Values; D012210:Rhamnose / Q000097:blood; D013021:South Australia; D013182:Sprue, Tropical / Q000175:diagnosis* / Q000503:physiopathology; D013395:Sucrose
chemical_list: D002250:Carbon Radioisotopes; D007792:Lactulose; D013395:Sucrose; D012210:Rhamnose