Antigenicity of a type A influenza virus through comparison of hemagglutination inhibition and mass spectrometry immunoassays.
The antigenicity of a type A (H1N1) influenza strain has been characterised through the application of mass spectrometry (MS) and hemagglutination-inhibition immunoassays performed in parallel. Two monoclonal antibodies were found to be highly and equally specific in HI assays against influenza strain A/New Caledonia/20/99 while the MS immunoassay demonstrated that both antibodies recognise the same epitopic peptide localised to residues 225-232 of the hemagglutinin HA1 subunit whose C-terminal residues reside in close proximity to the receptor binding site. Both immunoassays showed no binding of a monoclonal antibody that recognizes the hemagglutinin antigen of type B strains.
Schwahn
Alexander B
AB
School of Molecular & Microbial Biosciences, University of Sydney, Australia.
Downard
Kevin M
KM
D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D007118:Immunoassay / Q000379:methods*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D008958:Models, Molecular; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral