Healing of extraction sockets in collagenase-2 (matrix metalloproteinase-8)-deficient mice.
Matrix metalloproteinase-8 (MMP-8) participates in skin wound healing and inflammation. We hypothesized that MMP-8 plays a role in wound healing after tooth extraction and in periapical inflammation. Bone formation, collagen metabolism, and inflammation in tooth extraction socket and in periapical lesions were analyzed in wild-type mice and in MMP-8-deficient (MMP-8(-/-)) mice. New trabecular bone area in the extraction sockets and in periapical lesions were similar in both groups. In extraction sockets significantly more type III procollagen was synthesized, and the neutrophil and MMP-9 levels were lower in MMP-8(-/-) mice. The amount of Fas ligand, identified as a substrate for MMP-8, was lower in alveolar mucosa but higher in alveolar bone of MMP-8(-/-) mice. These results indicate that MMP-8 can modulate inflammation and collagen metabolism of alveolar bone and mucosa.
Korpi
Jarkko T
JT
Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, Finland.
Aström
Pirjo
P
Lehtonen
Niko
N
Tjäderhane
Leo
L
Kallio-Pulkkinen
Soili
S
Siponen
Maria
M
Sorsa
Timo
T
Pirilä
Emma
E
Salo
Tuula
T
D000539:Alveolar Process / Q000201:enzymology; D000818:Animals; D054428:Chemokine CX3CL1 / Q000032:analysis; D003094:Collagen / Q000378:metabolism; D024061:Collagen Type III / Q000096:biosynthesis; D053222:Fas Ligand Protein / Q000032:analysis; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D007371:Interferon-gamma / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000172:deficiency*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009061:Mouth Mucosa / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010483:Periapical Diseases / Q000201:enzymology / Q000503:physiopathology; D011347:Procollagen / Q000096:biosynthesis; D014081:Tooth Extraction; D020390:Tooth Socket / Q000201:enzymology* / Q000503:physiopathology; D014945:Wound Healing / Q000502:physiology
D054428:Chemokine CX3CL1; D024061:Collagen Type III; D053222:Fas Ligand Protein; D015850:Interleukin-6; D011347:Procollagen; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003094:Collagen; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse