Effect of PVP on sperm capacitation status and embryonic development in cattle.
The objective of this study was to investigate the effects of polyvinylpyrrolidone (PVP) on sperm capacitation status and embryonic development in cattle (Bos taurus). Acrosome-reacted sperm (chlortetracycline stain) and the fertilization rate after intracytoplasmic sperm injection (ICSI) were enhanced (P<0.05) when sperm were cultured in a medium containing 10% PVP. However, injection of bovine in vitro-produced (IVP) embryos with medium containing 10% PVP suppressed development of these embryos to the 2-cell, morula, and blastocyst stages and cell number at the blastocyst stage (P<0.01) but did not affect chromosomal integrity (P>0.05). Embryo developmental capacity differed (P<0.01) among PVP from three suppliers, but there were no significant differences among three solvent media. The PVP remained localized in 40.9% of PVP-injected IVP embryos. In conclusion, PVP affected the acrosome reaction and enhanced the fertilization rate after ICSI. However, PVP remained detectable in IVP embryos and suppressed their developmental capacity.
Kato
Y
Y
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Japan.
Nagao
Y
Y
D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D002417:Cattle / Q000196:embryology / Q000502:physiology; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D008297:Male; D011205:Povidone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D011247:Pregnancy; D013075:Sperm Capacitation / Q000187:drug effects*; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects
C077842:polyvinylpolypyrrolidone; D011205:Povidone