Chemical components and tyrosinase inhibitors from the twigs of Artocarpus heterophyllus.
An HPLC method was developed and validated to compare the chemical profiles and tyrosinase inhibitors in the woods, twigs, roots, and leaves of Artocarpus heterophyllus . Five active tyrosinase inhibitors including dihydromorin, steppogenin, norartocarpetin, artocarpanone, and artocarpesin were used as marker compounds in this HPLC method. It was discovered that the chemical profiles of A. heterophyllus twigs and woods are quite different. Systematic chromatographic methods were further applied to purify the chemicals in the twigs of A. heterophyllus. Four new phenolic compounds, including one isoprenylated 2-arylbenzofuran derivative, artoheterophyllin A (1), and three isoprenylated flavonoids, artoheterophyllin B (2), artoheterophyllin C (3), and artoheterophyllin D (4), together with 16 known compounds, were isolated from the ethanol extract of the twigs of A. heterophyllus. The structures of compounds 1-4 were elucidated by spectroscopic analysis. However, the four new compounds did not show significant inhibitory activities against mushroom tyrosinase compared to kojic acid. It was found that similar compounds, such as norartocarpetin and artocarpesin in the twigs and woods of A. heterophyllus, contributed to their tyrosinase inhibitory activity.
Zheng
Zong-Ping
ZP
School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
Chen
Sibao
S
Wang
Shiyun
S
Wang
Xia-Chang
XC
Cheng
Ka-Wing
KW
Wu
Jia-Jun
JJ
Yang
Dajiang
D
Wang
Mingfu
M
D000363:Agaricales / Q000201:enzymology; D031622:Artocarpus / Q000737:chemistry*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
D005656:Fungal Proteins; D010455:Peptides; D010936:Plant Extracts; D014442:Monophenol Monooxygenase