A single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus Gn glycoprotein confers protective immunity against seoul virus in mice.
Seoul virus (SEOV), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) and afflicts tens of thousands of people annually. In this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gn protein of SEOV (rCAV-2-Gn) in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against a lethal challenge with the highly virulent SEOV strain CC-2. Protective level neutralizing antibodies were maintained for at least 20 weeks. The efficacy of the recombinant was similar to that induced by a currently available inactivated HFRS vaccine. This recombinant virus is therefore a potential alternative to the inactivated vaccine.
Yuan
Zi-Guo
ZG
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tianhe District, Guangzhou 510642, Guangdong Province, PR China.
Li
Xiu-Ming
XM
Mahmmod
Yasser Saad
YS
Wang
Xiao-Hu
XH
Xu
Hui-Juan
HJ
Zhang
Xiu-Xiang
XX
D006516:Adenoviruses, Canine / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004285:Dogs; D005260:Female; D006386:Hemagglutination Tests; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology / Q000517:prevention & control*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D029263:Seoul virus / Q000276:immunology*; D014709:Vero Cells; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines