title: Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA.
abstract: Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells.
authors: Cornell University, Ithaca, New York, USA.
authors: Michael J
authors: MJ
authors: Scanlon
authors: Kazuhiro
authors: K
authors: Ohtsu
authors: Marja C P
authors: MC
authors: Timmermans
authors: Patrick S
authors: PS
authors: Schnable
mesh_terms: D042282:Microdissection / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics
chemical_list: D018749:RNA, Plant