Plasminogen activator inhibitor type-1 gene 4G/5G polymorphism in Turkish adult patients with asthma.
OBJECTIVE
This study has been performed on asthmatic patients in the Turkish population to determine the frequency of 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene, and with the aim of examining the role of this polymorphism in asthma development.


METHODS
Genomic DNA obtained from 165 persons (98 patients with asthma and 67 healthy controls) was used in the study. DNA was multiplied with polymerase chain reaction using 4G and 5G allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. Results were evaluated with chi-square test.


RESULTS
No statistically significant difference between the groups with respect to genotype distribution was found (p > 0.05) in the study. The 4G allele frequency was indicated as 48% and 5G allele was as 52% in patients, whereas this was 50-50% in the control group.


CONCLUSIONS
It has been established by this study that 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene do not play a role in the development of asthma in the Turkish population.
Cosan
Didem
D
Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey. dcosan@ogu.edu.tr
Kurt
Emel
E
Kurt
Hulyam
H
Degirmenci
Irfan
I
Kuçukarabaci
Banu
B
Metintas
Muzaffer
M
Kucuk
Meral Urhan
MU
Gunes
Hasan Veysi
HV
Colak
Ertugrul
E
D000328:Adult; D000483:Alleles; D001249:Asthma / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014421:Turkey / Q000453:epidemiology
D017395:Plasminogen Activator Inhibitor 1