Chemical study, antimalarial and antioxidant activities, and cytotoxicity to human breast cancer cells (MCF7) of Argania spinosa.
BACKGROUND
In our work, we evaluate the potential antioxidant, antimalarial activity and also activity against human breast cancer cells (MCF7) of Argan fruit extracts using in vitro models to validate the traditional use of this plant. Its chemical composition was also studied to begin the understanding of its activities, waiting to find the structure-activity relationship.


RESULTS
Polyphenols (89.4-218.5 eqGallic acid (mg/g dry)), tannins (39.3-214.0 eqCatechin (mg/g dry)), flavonoids (3.4-11.1 eqQuercetin (mg/g dry)) and anthocyanins (0.74-10.92 eqCyanindin (mug/g dry)) were quantified. A good (ethyl acetate and decoction) and moderate (petroleum ether) antioxidant activity were obtained for DPPH (IC(50) 32.3-600.8 microg/ml) and ABTS (IC(50) 11.9-988.8 microg/ml) assays. In addition, we found a good antimalarial activity (IC(50) 35 to >100 microg/ml) and human breast cancer cells activity (IC(50) 42 to >100 microg/ml).


CONCLUSIONS
The ethyl acetate extract and the decoction show interesting antimalarial and antioxidant activities. The results indicate a good correlations between anthocyanins quantitiy and the potential antioxidant (R(2)=0.9867) and also to antimalarial activity (R(2)=0.8175).
El Babili
Fatiha
F
Faculté des Sciences Pharmaceutiques. UMR IRD/UPS 152. 35 ch. des Maraîchers, F-31062 Toulouse, France. elbabili@cict.fr
Bouajila
Jalloul
J
Fouraste
Isabelle
I
Valentin
Alexis
A
Mauret
Severine
S
Moulis
Claude
C
D000962:Antimalarials / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D005260:Female; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D005638:Fruit; D006801:Humans; D020128:Inhibitory Concentration 50; D010636:Phenols / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D010963:Plasmodium falciparum / Q000187:drug effects; D029632:Sapotaceae / Q000737:chemistry*; D013451:Sulfonic Acids; D013634:Tannins / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use
D000962:Antimalarials; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013634:Tannins; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl