Folic acid supplementation modifies beta-adrenoceptor-mediated in vitro lipolysis of obese/diabetic (+db/+db) mice.
The effects of folic acid (5.7 and 71 microg/kg, 4 weeks) consumption on the beta-adrenoceptors (beta-ARs)-elicited lipolysis in vitro of the abdominal adipocytes of lean/control (+m/+db) and obese/diabetic (+db/+db) mice (female) were investigated. beta-AR agonists (salbutamol, a beta(2)-AR agonist; BRL 37344 and CGP 12177, beta(3)-AR agonists; adrenaline, a beta-AR agonist)-mediated lipolysis, beta(2)-, and beta(3)-ARs protein expression of the adipose tissues after folic acid consumption were evaluated. Our results demonstrate that a smaller magnitude of the basal (spontaneous) and the beta-AR agonists-triggered lipolysis was observed in +db/+db mice, and folic acid supplementation (71 microg/kg) resulted in an improvement of both the baseline and the beta-ARs-mediated lipolysis. In controls, a lower beta(2)-and beta(3)-ARs protein expression of the adipose tissues was detected in +db/+db mice, compared to +m/+db mice. In both strains fed with folic acid (71 microg/kg), a reduction of beta(2)-AR protein expression was observed compared to the respective controls. In +db/+db mice, folic acid (5.7 and 71 microg/kg) consumption caused a dose-dependent increase of beta(3)-AR protein expression compared to controls. We demonstrate that lipolysis elicited by beta-AR (beta(2)- and beta(3)-ARs) agonists was blunted in +db/+db mice. Folic acid consumption has significant modulatory effects on beta-ARs protein expression and lipolysis.
Lam
Tsz Yan
TY
Li Ka Shing Institute of Health Sciences, University of Hong Kong, Shatin, N.T., Hong Kong, PR China.
Seto
Sai Wang
SW
Au
Alice Lai Shan
AL
Poon
Christina Chui Wa
CC
Li
Rachel Wai Sum
RW
Lam
Ho Yeung
HY
Lau
Wing Sze
WS
Chan
Shun Wan
SW
Ngai
Sai Ming
SM
Leung
George Pak Heng
GP
Lee
Simon Ming Yuen
SM
Tsui
Stephen Kwok Wing
SK
Kwan
Yiu Wa
YW
D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005492:Folic Acid / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D011941:Receptors, Adrenergic / Q000187:drug effects*; D054411:Receptors, Leptin / Q000235:genetics*; D014815:Vitamins / Q000494:pharmacology*
D011941:Receptors, Adrenergic; D054411:Receptors, Leptin; D014815:Vitamins; C515447:leptin receptor, mouse; D005492:Folic Acid