[Matrix metalloproteinases and their tissue inhibitors in children and young adults on chronic hemodialysis--preliminary results].
The proteolytic system of MMPs and TIMPs may play an essential role in vascular remodeling and atherosclerosis, but the data concerning such influence in the patients on chronic hemodialysis are scarce. The aim of the study was to assess the concentrations of selected MMPs and their inhibitors in the patients hemodialyzed on the polysulfone membranes.


METHODS
11 children and young adults on chronic HD were enrolled in the study, 15 age-matched subjects with normal kidney function served as controls. The serum concentrations of MMP-2, MMP-9, TIMP-1 and TIMP-2 were assessed by ELISA.


RESULTS
The median values of all MMPs and TIMPs were elevated in patients before HD vs. controls (p<0.0001) and decreased after a single HD session (p<0.01), yet remained higher than in the control group (p<0.001). The MMP-2/TIMP-2 ratio was decreased vs. controls both before and after a HD session (p<0.0001). Contrarily, the MMP-9/TIMP-1 ratio before HD was higher than in controls (p<0.05), but after HD dropped to the values lower than those in the control subjects (p<0.01).


CONCLUSIONS
The increased concentrations of all parameters may indicate the proteolytic overactivity in the examined population. A single polysulfone HD session tends to deepen the MMPs/TIMPs dysfunction, although this impact seems transient.
Musiał
Kinga
K
Department of Pediatric Nephrology of Medical University of Wrocław. kinga_musial@hotmail.com
Makulska
Irena
I
Zwolńska
Danuta
D
D000293:Adolescent; D001672:Biocompatible Materials; D002648:Child; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy*; D008297:Male; D020782:Matrix Metalloproteinases / Q000097:blood*; D008567:Membranes, Artificial; D011108:Polymers; D006435:Renal Dialysis; D013450:Sulfones; D019714:Tissue Inhibitor of Metalloproteinases / Q000097:blood*; D055815:Young Adult
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; D019714:Tissue Inhibitor of Metalloproteinases; C017662:polysulfone P 1700; D020782:Matrix Metalloproteinases