[Mutation analysis of neural cell adhesion molecules in human astrocytoma].
OBJECTIVE
To identify the function of neural cell adhesion molecule (NCAM) mutation in the genesis of human glioma.


METHODS
Mutations were found through polymerase chain reaction-single strand conformation polymorphism, and then the changed DNA fragments were purified and multiplied and sent to Shanghai for sequencing. Blast and ORF finder were used to find out the amino changes in NCAM.


RESULTS
An A-C transversion was found at position 1 126 in NCAM's 7 exon in a patient with glioblastoma from 43 astrocytoma.


CONCLUSIONS
Structural change in the protein caused by point mutation may be the reason for tumorigenesis of astrocytoma.
Wang
Yanjin
Y
Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 400008, China.
Xiang
Juanjuan
J
Fang
Jiasheng
J
D000328:Adult; D001254:Astrocytoma / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D019002:CD56 Antigen / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation
D019002:CD56 Antigen; C522918:NCAM1 protein, human