title: [Study on the correlation between CD14 gene polymorphism and T cell-mediated immunity in severely burned patients].
abstract: OBJECTIVE
  To investigate the correlation between CD14 gene polymorphism and T cell-mediated immunity in severely burned patients.
  
  
  METHODS
  The blood samples of 77 patients with extensive burn injury (> 30% total body surface area) were collected, and CD14-159C/T gene polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). T lymphocyte cell proliferation and interleukin-2 (IL-2) production were determined, and the ratio of CD4(+)/CD8(+) T lymphocyte as well as apoptosis of CD4(+) T lymphocyte was examined by flow cytometry.
  
  
  RESULTS
  The ability of T lymphocyte proliferation was obviously decreased in severely burned patients. Compared with CC homozygote patients, proliferative activity of T lymphocyte to mitogen stimulation was significantly depressed in TT and TC patients on post burn days 5, 21, and 28 (P < 0.05 or P < 0.01). IL-2 production in TT, TC patients was constantly in low level after burns, while it was increased from post burn day 14 in CC patients. The ratio of CD4(+)/CD8(+) T lymphocytes was markedly decreased in TC, TT patients than that in CC patients, especially on post burn days 1, 3, 14, 21, and 28 (P < 0.05 or P < 0.01). Meanwhile, compared with CC homozygote patients, the apoptosis rates of CD3(+)CD4(+) T lymphocytes were much higher in TT patients on post burn days 5, 7, and 21 (P < 0.05), and in TC patients on days 7, 14 (P < 0.05), respectively. However, no obvious differences in parameters of immune function of T lymphocytes were found between TT and TC patients (P > 0.05).
  
  
  CONCLUSIONS
  CD14-159C/T polymorphism could influence the T cell-mediated immunity in extensively burned patients, which might participate in the development of septic complications secondary to major burns.
authors: Burns Institute, First Hospital Affiliated to the General Hospital of the People's Liberation Army, Beijing 100037, China.
authors: Ning
authors: N
authors: Dong
authors: Yong-ming
authors: YM
authors: Yao
authors: Yan
authors: Y
authors: Yu
authors: Yu-jue
authors: YJ
authors: Cao
authors: Li-xin
authors: LX
authors: He
authors: Hong-ming
authors: HM
authors: Yang
authors: Zhi-yong
authors: ZY
authors: Sheng
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D002056:Burns / Q000235:genetics / Q000276:immunology*; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D055815:Young Adult
chemical_list: D007376:Interleukin-2; D018950:Lipopolysaccharide Receptors