title: Transcription factor GATA-3 in Atlantic salmon (Salmo salar): molecular characterization, promoter activity and expression analysis.
abstract: GATA-3 is a T cell-specific transcription factor and is essential for the development of the T cell lineage and differentiation of T helper type 2 cells. We have identified and characterized the full-length Atlantic salmon GATA-3 cDNA (3074bp), having two zinc finger domains which are fully conserved within teleosts and higher vertebrates. RT-PCR analysis revealed that the Atlantic salmon GATA-3 (AsGATA-3) is strongly expressed in gills, thymus, and brain. Moreover, the involvement of GATA-3 in Atlantic salmon immune response was demonstrated by investigating the early time dependent expression profile of GATA-3 in spleen and head kidney following intraperitoneal injection of live Aeromonas salmonicida, LPS, and beta-glucan. Furthermore, we have determined 1.9kb of upstream promoter sequence and found a number of sequence motifs which match those of known transcription factor binding sites and the AsGATA-3 promoter is a TATA-less promoter. Activities of presumptive regulatory regions of this gene were assessed by transfecting different 5' deletion constructs and the result showed the basal promoter and positive transcriptional regulator activity of AsGATA-3 gene is determined by sequences located between +58 and -199bp upstream of the transcriptional start site (TSS). This study provides further insights into the transcriptional regulation of AsGATA-3.
authors: Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, N-9037 Tromsø, Norway. jaya.kumari@uit.no
authors: Jaya
authors: J
authors: Kumari
authors: Jarl
authors: J
authors: Bogwald
authors: Roy A
authors: RA
authors: Dalmo
mesh_terms: D048409:Aeromonas salmonicida; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D050990:GATA3 Transcription Factor / Q000819:agonists / Q000096:biosynthesis* / Q000737:chemistry; D017930:Genes, Reporter / Q000235:genetics / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D006367:HeLa Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D020317:Salmo salar / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D014162:Transfection; D047071:beta-Glucans / Q000494:pharmacology
chemical_list: D050990:GATA3 Transcription Factor; D008070:Lipopolysaccharides; D047071:beta-Glucans