title: Features of intervertebral disc degeneration in rat's aging process.
abstract: OBJECTIVE
  The age-related change is important part of degenerative disc disease. However, no appropriate animal model or objective evaluation index is available. This study aimed to investigate the features of intervertebral disc degeneration in aging process of rats.
  
  
  METHODS
  22-month-old Sprague-Dawley (SD) rats were used as spontaneously occurring intervertebral disc degeneration models and 6-month-old rats as young controls. Expression of collagen types II and X was measured by immunohistochemistry. Degenerations of intervertebral discs were scored according to Miyamoto's method. Numbers and areas of afferent vascular buds were measured. The thicknesses of non-calcified and calcified layers were measured and statistically analyzed.
  
  
  RESULTS
  There were less collagen type II expression and more collagen type X expression in the calcified layer of the cartilage endplates and nucleus pulposus in the rats of the aged group than in the young control. There were fewer and smaller afferent vascular buds in the rats of the aged group than in the young control group. The ratio of the non-calcified to the calcified layers in the rats of the aged group significantly decreased, compared with that of the young control group (P<0.01).
  
  
  CONCLUSIONS
  Rats can spontaneously establish intervertebral disc age-related degeneration. The expression of collagen types II and X, numbers and areas of afferent vascular buds, the ratio of the non-calcified to the calcified layers, and water and glycosaminoglycan contents in the nucleus pulposus are sensitive indexes of intervertebral disc degeneration.
authors: Department of Orthopaedics, the First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061, China; zyingang@mail.xjtu.edu.cn.
authors: Yin-Gang
authors: YG
authors: Zhang
authors: Zheng-Ming
authors: ZM
authors: Sun
authors: Jiang-Tao
authors: JT
authors: Liu
authors: Shi-Jie
authors: SJ
authors: Wang
authors: Feng-Ling
authors: FL
authors: Ren
authors: Xiong
authors: X
authors: Guo
mesh_terms: D000375:Aging / Q000473:pathology*; D000818:Animals; D005260:Female; D007403:Intervertebral Disc / Q000473:pathology* / Q000503:physiopathology*; D007405:Intervertebral Disc Displacement / Q000473:pathology* / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley