Transgenic lettuce producing a candidate protein for vaccine against edema disease.
Pig edema disease is a bacterial disease caused by Shiga toxin 2e-producing Escherichia coli belonging mainly to serotypes O138, O139, and O141. The B subunit of Shiga toxin 2e (Stx2eB) is a candidate protein for use in a vaccine against edema disease. We produced this protein in transgenic lettuce (Lactuca sativa), an edible plant that can be cultivated in a factory setting. In a transient expression system, we found that NtADH 5'-untranslated region (5'-UTR) functions as a translational enhancer in lettuce cells, and that Stx2eB accumulates most efficiently in the endoplasmic reticulum (ER) of lettuce cells. Stx2eB was produced in stable transgenic lettuce plants expressing a modified Stx2eB gene fused with the NtADH 5'-UTR and sequence encoding ER localization signals.
Matsui
Takeshi
T
Advanced Technology Research Laboratories, Idemitsu Kosan Co, Ltd, Sodegaura, Chiba, Japan.
Asao
Hiroshi
H
Ki
Misa
M
Sawada
Kazutoshi
K
Kato
Ko
K
D020121:5' Untranslated Regions / Q000235:genetics; D000426:Alcohol Dehydrogenase / Q000235:genetics; D000818:Animals; D001428:Bacterial Vaccines / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004487:Edema / Q000276:immunology* / Q000517:prevention & control; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D018545:Lactuca / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*; D021381:Protein Transport; D015203:Reproducibility of Results; D022641:Shiga Toxin 2 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D034421:Sus scrofa / Q000276:immunology / Q000382:microbiology; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control; D014026:Nicotiana / Q000201:enzymology; D022462:Vaccines, Edible / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D020121:5' Untranslated Regions; D001428:Bacterial Vaccines; D022641:Shiga Toxin 2; D022462:Vaccines, Edible; D000426:Alcohol Dehydrogenase