Effects of IAA in combination with FSH on in vitro culture of ovine preantral follicles.
Ovarian cortical fragments from five adult ewes were in vitro cultured for 1, 3 or 5 days in the presence of minimum essential medium either supplemented or not by follicle-stimulating hormone (FSH) (100 ng/ml) or indole-3-acetic acid (IAA) (10, 20, 40 or 100 ng/ml), alone or in combination. After in vitro culture, ovarian fragments were submitted to follicular isolation and viability test was performed using trypan blue. Addition of IAA (10 ng/ml) to a free-FSH medium resulted in the highest percentages of viable follicles, but was progressively deleterious in higher concentrations (20, 40 and 100 ng/ml) if in absence of FSH. Follicular development was observed only when FSH was added to an IAA-free medium. In conclusion, IAA at a concentration of 10 ng/ml increases follicular survival in vitro. However, at high concentrations (20, 40 or 100 ng/ml), this auxin may be deleterious to preantral follicles, the addition of FSH to the medium being necessary.
Costa
Sonia H F
SH
LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Santos
Regiane R
RR
Rondina
Davide
D
Andrade
Evelyn R
ER
Ohashi
Otávio M
OM
Rodrigues
Ana Paula R
AP
Figueiredo
José R
JR
D000818:Animals; D002470:Cell Survival; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects*; D012756:Sheep; D046509:Tissue Culture Techniques
D007210:Indoleacetic Acids; C030737:indoleacetic acid; D005640:Follicle Stimulating Hormone