title: Novel IgE recognized components of Lolium perenne pollen extract: comparative proteomics evaluation of allergic patients sensitization profiles.
abstract: In the last years, proteomic investigation provided a powerful tool in molecular characterization of complex allergen sources with relevant implications in both diagnosis and immunotherapic treatment of allergies. We followed a proteomic approach to characterize ryegrass (Lolium perenne) pollen, a common cause of seasonal allergic diseases affecting an increasing part of world population. Peptide shotgun experiments performed on nanoLiquid Ultra Pressure Chromatography coupled with fast Q-TOF MS-MS/MS acquisition protocols (MS(E)) and 2-DE immunoblot combined with MALDI-TOF-TOF analysis allowed the detection of all previously identified ryegrass allergens. Comparative analysis of immunoblot highlighted a class of patients characterized by a more complex 2-DE pattern associated with increased levels of IgE antibodies and by higher susceptibility to multiple sensitization toward different allergen sources. Cluster analysis revealed that all these patients recognized profilin, considered the main cross-reactive allergen in grass pollen. Furthermore, mass spectrometry analysis revealed the presence of other IgE reactive components in ryegrass pollen that might be involved in polysensitization, such as cyclophilin, fructosyltransferase and legumin-like protein.
authors: Department of Internal Medicine, University of Rome Tor Vergata, Rome, Italy.
authors: Michele
authors: M
authors: De Canio
authors: Simona
authors: S
authors: D'Aguanno
authors: Cristiano
authors: C
authors: Sacchetti
authors: Francesca
authors: F
authors: Petrucci
authors: Giovanni
authors: G
authors: Cavagni
authors: Marzia
authors: M
authors: Nuccetelli
authors: Giorgio
authors: G
authors: Federici
authors: Andrea
authors: A
authors: Urbani
authors: Sergio
authors: S
authors: Bernardini
mesh_terms: D016000:Cluster Analysis; D015331:Cohort Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D008129:Lolium / Q000737:chemistry / Q000276:immunology*; D015999:Multivariate Analysis; D011058:Pollen / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D007073:Immunoglobulin E