Bioencapsulation of apomyoglobin in nanoporous organosilica sol-gel glasses: influence of the siloxane network on the conformation and stability of a model protein.
Nanoporous sol-gel glasses were used as host materials for the encapsulation of apomyoglobin, a model protein employed to probe in a rational manner the important factors that influence the protein conformation and stability in silica-based materials. The transparent glasses were prepared from tetramethoxysilane (TMOS) and modified with a series of mono-, di- and tri-substituted alkoxysilanes, R(n)Si(OCH(3))(4-n) (R = methyl-, n = 1; 2; 3) of different molar content (5, 10, 15%) to obtain the decrease of the siloxane linkage (-Si-O-Si-). The conformation and thermal stability of apomyoglobin characterized by circular dichroism spectroscopy (CD) was related to the structure of the silica host matrix characterized by (29)Si MAS NMR and N(2) adsorption. We observed that the protein transits from an unfolded state in unmodified glass (TMOS) to a native-like helical state in the organically modified glasses, but also that the secondary structure of the protein was enhanced by the decrease of the siloxane network with the methyl modification (n = 0 < n = 1 < n = 2 < n = 3; 0 < 5 < 10 < 15 mol %). In 15% trimethyl-modified glass, the protein even reached a maximum molar helicity (-24,000 deg. cm(2) mol(-1)) comparable to the stable folded heme-bound holoprotein in solution. The protein conformation and stability induced by the change of its microlocal environment (surface hydration, crowding effects, microstructure of the host matrix) were discussed owing to this trend dependency. These results can have an important impact for the design of new efficient biomaterials (sensors or implanted devices) in which properly folded protein is necessary.
Menaa
Bouzid
B
Department of Chemistry, Duncan Hall, San José State University, San José, CA 95112-0101, USA. bouzid.menaa@gmail.com
Miyagawa
Yuya
Y
Takahashi
Masahide
M
Herrero
Mar
M
Rives
Vicente
V
Menaa
Farid
F
Eggers
Daryl K
DK
D000818:Animals; D001059:Apoproteins / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D055598:Chemical Phenomena; D005898:Glass / Q000737:chemistry*; D006736:Horses; D009211:Myoglobin / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D044367:Phase Transition; D016062:Porosity; D011487:Protein Conformation; D017510:Protein Folding; D055550:Protein Stability; D012821:Silanes / Q000737:chemistry; D017655:Silicon Compounds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D001059:Apoproteins; D001672:Biocompatible Materials; D009211:Myoglobin; D017646:Organosilicon Compounds; D012821:Silanes; D017655:Silicon Compounds; C010857:apomyoglobin; D014867:Water; C071717:trimethoxysilane