Expression of JNK-interacting protein JIP-1 and insulin-like growth factor II in Wilms tumour cell lines and primary Wilms tumours.
JNK-interacting protein 1 (JIP-1) is an important scaffolding protein in the JNK signalling pathway. It is also believed to play a role in the mediation of mitogenic messages from the plasma membrane to the cell interior. Previous studies suggest that the JIP-gene is co-regulated with the insulin-like growth factor II (IGF II) gene, thereby contributing to the growth stimulatory effects of this potent growth factor. The striking coexpression of these two genes was found in murine fetuses as well as in primary human embryonic tumours. When six primary Wilms tumours were examined, the two genes showed a high degree of co-variation in the sense that high expression of IGF II was followed by high expression of JIP-1 and vice versa. However, when the human Wilms tumour cell line WCCS-1 was examined, a very modest intrinsic expression of IGF II was accompanied by a moderate expression of JIP-1. When exogenous IGF II was added, which has previously been shown to induce apoptosis in this cell line, the JIP-1 expression increased. These data suggest that JIP-1 has a more complex role in the regulation of proliferation as well as programmed cell death.
Engström
Wilhelm
W
Division of Pathology, Department of Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden. wilhelm.engstrom@bvf.slu.se
Granerus
Marika
M
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism; D009396:Wilms Tumor / Q000378:metabolism* / Q000473:pathology
D048868:Adaptor Proteins, Signal Transducing; C490666:MAPK8IP1 protein, human; D011994:Recombinant Proteins; D007335:Insulin-Like Growth Factor II