[Effects of cardiopulmonary bypass on plasma pentraxin-3 level and the balance of Th1/Th2 function in children with congenital heart malformations].
OBJECTIVE
To investigate the effects of cardiopulmonary bypass (CPB) on plasma pentraxin-3 (PTX3) level and balance of Th1/Th2 function in children with congenital heart malformations.


METHODS
16 children with congenital heart malformations, 9 male and 7 female, aged 3 - 52 months, weighing 3.4 - 14.5 kilograms, classified as II or III ASA grades, underwent operation under CPB. Blood samples were collected after anesthesia induction, 5 min and 30 min since beginning of CPB, and 5 min, 2 h, 4 h, 8 h, and 16 h after weaning from CPB. ELISA was used to examine the plasma concentrations of interferon (INF)-gamma, interleukin (IL)-4, and PTX3. The ratio of INF-gamma/IL-4 was calculated.


RESULTS
The INF-gamma level began to increase significantly 5 min since CPB (P < 0.05), peaked 5 min after weaning from CPB (P < 0.01), then gradually decreased, and returned to the level before CPB 16 h after the weaning from CPB (P > 0.05). The IL-4 level began to increase significantly 30 min since CPB (P < 0.01), peaked 2 h since CPB (P < 0.01), however, still not returned to the level before CPB 16 h after the weaning from CPB (P < 0.05). The PTX-3 level was significantly higher 30 min since CPB and 5 min and 2 h after the weaning from CPB (all P < 0.01). The INF-gamma/IL-4 ratio was significantly higher 5 min and 30 min since CPB and 5 min after weaning from CPB (all P > 0.05).


CONCLUSIONS
CPB can leads to elevation of plasma concentration of PTX3 and drift of Th1/Th2 function equilibrium, thus resulting in immunity disequilibrium.
Wang
Shou-Yong
SY
Department of Anesthesiology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. wangshyong@163.com
Li
Ting-Yu
TY
Zheng
Yu-Qiang
YQ
D002097:C-Reactive Protein / Q000378:metabolism*; D006348:Cardiac Surgical Procedures; D002315:Cardiopulmonary Bypass; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D015847:Interleukin-4 / Q000097:blood / Q000378:metabolism; D008297:Male; D010949:Plasma / Q000737:chemistry; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*; D000099153:Pentraxins
D002097:C-Reactive Protein; D007371:Interferon-gamma; D015847:Interleukin-4; D000683:Serum Amyloid P-Component; D000099153:Pentraxins