[The empirical study in transplantation of bone marrow mesenchymal stem cells for acute myocardial infarction].
OBJECTIVE
To study the effect of (60)Co gamma ray on proliferation and differentiation potency of MSC. To evaluate the function of heart after early stage of acute myocardial infarction (AMI) in rats by transplantation of MSC.


METHODS
Irradiated MSC was cultured with the method of adherent culture, the ability of its proliferation was detected by Methyl thiazolyl tetrazolium (MTT) shade selection test. The express of Cardiac troponin T (C-TNT) and beta-myosin heavy chain (beta-MHC) in MSC were detected by Reverse transcriptase-polymerase chain reaction (RT-PCR), which was induced by 5-azacytidine. Using a rat model of AMI, irradiated and unirradiated MSC were transplanted into myocardium after infarction. At 4 weeks, the cardiac dimension and functions were assessed by echocardiography and hemodynamics, the microvessel density were detected after VIII factor immunohistochemistry stain.


RESULTS
The ability of proliferation in 4Gy group was depressed. C-TNT and beta-MHC expressed somewhat in unirradiated group. When compared with the control group (6.6 +/- 0.6), the microvessel density in both transplanted groups (12.7 +/- 2.2, 11.5 +/- 2.1) increased, contributing to improvement in cardiac function and dimension.


CONCLUSIONS
MSC with or without differentiation potency can improve cardiac function equally at the early stage of AMI.
Ma
Xiao-Hui
XH
The General Hospital of People's Liberation Army, Beijing 100853, China.
Gao
Chang-Qing
CQ
Li
Bo-Jun
BJ
Jiang
Sheng-Li
SL
Xiao
Cang-Song
CS
Wu
Yang
Y
D000818:Animals; D049109:Cell Proliferation; D045164:Mesenchymal Stem Cell Transplantation; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley