title: Focus on the controversial activation of human iNKT cells by 4-deoxy analogue of KRN7000.
abstract: 4-Deoxy-alpha-GalCer analogues are considered weaker agonists than KRN7000 for the stimulation of human iNKT cells, but this remains strongly debated. In this work, we described a strategy toward 4-deoxy-alpha-GalCers with, as a key step, a metathesis reaction allowing sphingosine modifications from a single ethylenic alpha-galactoside precursor. The 4-deoxy-KRN7000 derivative 2, described here, induced potent cytokinic responses, comparable to those of KRN7000, both from human iNKT cells in vitro and from their murine counterpart in vivo.
authors: Université de Nantes, CNRS, Chimie et Interdisciplinarité: Synthèse, Analyse, Modélisation (CEISAM), UMR CNRS 6230, Faculté des Sciences et des Techniques, 2 Rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors: Vivien
authors: V
authors: Lacône
authors: Julie
authors: J
authors: Hunault
authors: Muriel
authors: M
authors: Pipelier
authors: Virginie
authors: V
authors: Blot
authors: Thomas
authors: T
authors: Lecourt
authors: Jézabelle
authors: J
authors: Rocher
authors: Anne-Laure
authors: AL
authors: Turcot-Dubois
authors: Séverine
authors: S
authors: Marionneau
authors: Jean-Yves
authors: JY
authors: Douillard
authors: Monique
authors: M
authors: Clément
authors: Jacques
authors: J
authors: Le Pendu
authors: Marc
authors: M
authors: Bonneville
authors: Laurent
authors: L
authors: Micouin
authors: Didier
authors: D
authors: Dubreuil
mesh_terms: D000276:Adjuvants, Immunologic / Q000138:chemical synthesis / Q000494:pharmacology*; D000818:Animals; D005699:Galactosylceramides / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D055611:Natural Killer T-Cells / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000276:Adjuvants, Immunologic; D005699:Galactosylceramides; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma; C103873:KRN 7000