title: [Homocysteine and glutathione metabolism in steroid-treated relapse of idiopathic nephrotic syndrome].
abstract: Changes in metabolism of aminothiols may have an influence on endothelial function or change the red-ox balance. The aim of study was designed to assess changes in plasma aminothiols': proatherogenic (homocysteine-HCY) and antiatherogenic (glutathione-GSH) metabolism in nephrotic syndrome in children.
  
  
  METHODS
  The study group included 77 nephrotic children (aged 2-18 years) divided into four groups, i.e. in acute phase of the disease (24), during steroid-induced (24), steroid-free (12) and in long-term remission (17). Twenty five healthy children served as controls. GSH and HCY in plasma were assessed by high-performance liquid chromatography. Fraction of protein-bound and free aminothiols was assessed and albumin saturation was calculated.
  
  
  RESULTS
  GSH and its fractions' concentrations were comparable to healthy subject, however in early relapse free fraction was significantly higher than in late remission. The albumin saturation with GSH was significantly higher in early than in late relapse. Total HCY concentration was decreased in early relapse, elevated after 2 week and comparable to controls after 8 week of treatment. HCY free fraction and albumin saturation were elevated within first 2 weeks. Children in long-term remission showed elevated total concentration of HCY and GSH and their protein bound fractions when compared to controls. Albumin saturation with these aminoacids was higher as well.
  
  
  CONCLUSIONS
  The study showed aminothiol imbalance in children in first weeks of relapse of nephrotic syndrome.
authors: Department of Nephrology and Dialysis, Polish Mother's Memorial Hospital Research Institute, Lódź. iczmp.nefro@wp.pl
authors: Marcin
authors: M
authors: Tkaczyk
authors: Aneta
authors: A
authors: Czupryniak
authors: Michał
authors: M
authors: Nowicki
authors: Grazyna
authors: G
authors: Chwatko
authors: Edward
authors: E
authors: Bald
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005978:Glutathione / Q000097:blood*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D007223:Infant; D008297:Male; D009404:Nephrotic Syndrome / Q000097:blood* / Q000188:drug therapy*; D012008:Recurrence; D013256:Steroids / Q000627:therapeutic use
chemical_list: D013256:Steroids; D006710:Homocysteine; D005978:Glutathione