Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection.
White spot syndrome virus (WSSV) can cause the most serious viral disease of shrimp and has a wide host range among crustaceans. Although researches show a lot about its genome and structure, information concerning the mechanism of how WSSV infects' cells is lacking. In this study, some experiments were applied to confirm the biological meaning of the protein-protein interaction between WSSV envelope protein, VP53A, and Penaeus monodon chitin-binding protein (PmCBP). Immunofluorescent study indicated that PmCBP is located on the cell surface of host cells. PmCBP amounts of about 34kDa can be detected in both P. monodon and Litopenaeus vannamei tissues by Western blotting. In the in vivo neutralization experiment, both rVP53A and rPmCBP that were produced by Esherichia coli can promote resp. a 40% and 20% survival rate of the shrimp which were challenged by WSSV. Furthermore, a yeast-two-hybrid result revealed that PmCBP could interact with at least 11 WSSV envelope proteins. Those findings suggest that PmCBP may be involved in WSSV infection.
Chen
Kuan-Yu
KY
Institute of Marine Biology, National Taiwan Ocean University, Jhongjheng District, Keelung City 20224, Taiwan, ROC.
Hsu
Tai-Ching
TC
Huang
Po-Yu
PY
Kang
Shih-Ting
ST
Lo
Chu-Fang
CF
Huang
Wei-Pang
WP
Chen
Li-Li
LL
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000502:physiology*
D002352:Carrier Proteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins