The occurrence of multidrug-resistant Pseudomonas aeruginosa on hydrocarbon-contaminated sites.
The main aim of this paper was the comprehensive estimation of the occurrence rate and the antibiotic-resistance conditions of opportunistic pathogen Pseudomonas aeruginosa in hydrocarbon-contaminated environments. From 2002 to 2007, 26 hydrocarbon-contaminated sites of Hungary were screened for the detection of environmental isolates. Altogether, 156 samples were collected and examined for the determination of appearance, representative cell counts, and antibiotic-resistance features of P. aeruginosa. The detected levels of minimal inhibitory concentrations of ten different drugs against 36 environmental strains were compared to the results of a widely used reference strain ATCC 27853 and four other clinical isolates of P. aeruginosa. Based on our long-term experiment, it can be established that species P. aeruginosa was detectable in case of 61.5% of the investigated hydrocarbon-contaminated sites and 35.2% of the examined samples that shows its widespread occurrence in polluted soil-groundwater systems. In the course of the antibiotic-resistance assay, our results determined that 11 of the examined 36 environmental strains had multiple drug-resistance against several clinically effective antimicrobial classes: cephalosporins, wide spectrum penicillins, carbapenems, fluoroquinolones, and aminoglycosides. The fact that these multiresistant strains were isolated from 8 different hydrocarbon-contaminated sites, mainly from outskirts, confirms that multiple drug-resistance of P. aeruginosa is widespread not only in clinical, but also in natural surroundings as well.
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Regional University Center of Excellence, Szent István University, Gödöllo, Hungary.
Kriszt
Balázs
B
Atzél
Béla
B
Szabó
Gabriella
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Szabó
István
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Harkai
Péter
P
Szoboszlay
Sándor
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D024901:Drug Resistance, Multiple, Bacterial; D004784:Environmental Monitoring; D006814:Hungary; D006838:Hydrocarbons / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012989:Soil Pollutants / Q000032:analysis*
D006838:Hydrocarbons; D012989:Soil Pollutants