Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm characteristics.
Reports indicate an increase in the incidence of DNA fragmentation in male factor infertility and its role in the outcome of assisted reproductive techniques (ART). However, reports are conflicting between the relationships of sperm DNA integrity with conventional semen parameters. We examined the relationship between conventional sperm parameters and DNA integrity using acridine orange (AO) test. The study included 373 patients and 28 fertile volunteers. DNA normality was compared with semen parameters between the patient and donor populations. Significant correlations were noted between DNA normality and sperm concentration (r = 0.18, P = 0.000), motility (r = 0.21, P = 0.0001), rapid motility (0.19, P = 0.000), normal morphology by World Health Organization (r = 0.15, P = 0.019) and head defects (r = -0.15, P = 0.023). A significant difference was noted in AO levels between donors and patients with asthenozoospermia (P = 0.002) and oligoasthenozoospermia (P = 0.001). A significant difference in DNA integrity was noted in samples having <30% and >30% normal morphology. A wide range of % DNA normality was observed in the patient group. Sperm assessment for DNA status using AO is reliable and shows good correlation with sperm count, motility and morphology. Assessment of sperm DNA status with AO staining may be helpful prior to ART.
Varghese
A C
AC
Fertility Clinic and IVF Department, AMRI Medical Centre, Kolkata, India.
Bragais
F M
FM
Mukhopadhyay
D
D
Kundu
S
S
Pal
M
M
Bhattacharyya
A K
AK
Agarwal
A
A
D000165:Acridine Orange; D004247:DNA / Q000235:genetics*; D053938:DNA Fragmentation; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility / Q000235:genetics; D013094:Spermatozoa / Q000502:physiology*; D014019:Tissue Donors
D004247:DNA; D000165:Acridine Orange