Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum.
Hookworms, bloodfeeding intestinal nematodes, infect nearly one billion people in resource limited countries and are a leading cause of anaemia and malnutrition. Like other nematodes, hookworms lack the capacity to synthesise essential fatty acids de novo and therefore must acquire those from exogenous sources. The cDNA corresponding to a putative Ancylostoma ceylanicum fatty acid and retinol binding protein-1 (AceFAR-1) was amplified from adult hookworm mRNA. Studies using quantitative reverse transcriptase real-time PCR demonstrate that AceFAR-1 transcripts are most abundant in the earliest developmental stages of the parasite, and greater in females than males. Using in vitro assays, the recombinant AceFAR-1 (rAceFAR-1) was shown to bind individual fatty acids with equilibrium dissociation constants in the low micromolar range. The pattern of fatty acid uptake by live adult worms cultured ex vivo was similar to the in vitro binding profile of rAceFAR-1, raising the possibility that the native protein may be involved in acquisition of fatty acids by A. ceylanicum. Animals vaccinated orally with rAceFAR-1 and the mucosal adjuvant cholera toxin exhibited a statistically significant (40-47%) reduction in intestinal worm burden compared with controls immunized with antigen or adjuvant alone. Together, these data suggest a potential role for AceFAR-1 in hookworm biology, making it a potentially valuable target for drug and vaccine development.
Fairfax
Keke C
KC
Infectious Diseases Section and Program in International Child Health, Department of Pediatrics, Yale School of Medicine, New Haven, CT 06520, USA.
Vermeire
Jon J
JJ
Harrison
Lisa M
LM
Bungiro
Richard D
RD
Grant
Wayne
W
Husain
Sohail Z
SZ
Cappello
Michael
M
D000704:Analysis of Variance; D000722:Ancylostoma / Q000254:growth & development / Q000378:metabolism*; D000723:Ancylostomatoidea; D000818:Animals; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D008018:Life Cycle Stages; D008297:Male; D008969:Molecular Sequence Data; D012177:Retinol-Binding Proteins / Q000378:metabolism*
D018076:DNA, Complementary; D005227:Fatty Acids; D012177:Retinol-Binding Proteins