Labeling of BSA and imaging of mouse T-lymphocyte as well as mouse spleen tissue by L-glutathione capped CdTe quantum dots.
L-glutathione capped highly fluorescent CdTe quantum dots (QDs) were prepared by an aqueous approach and used as fluorescent labels to link albumin bovine serum (BSA) and rat anti-mouse CD4, which was expressed on mouse T-lymphocyte and mouse spleen tissue. The sharp and narrow emission peaks showed that the as-prepared QDs have desirable dispersibility, uniformity and good fluorescence properties. Both CdTe-BSA and CdTe-CD4 conjugates showed an enhancement of fluorescence intensity over that of bare CdTe QDs. The experimental result of gel electrophoresis confirmed the successful conjugation of CdTe-BSA and CdTe-CD4. The fluorescent microscopic images of CdTe-CD4 labeled mouse T-lymphocyte cells and mouse spleen tissue were compared with that obtained from fluorescein isothiocyanate labeling. It was demonstrated that the CdTe QDs-based probe exhibited much better photostability and fluorescence intensity than fluorescein isothiocyanate, showing a good application potential in the immuno-labeling of cells and tissues.
Dong
Wei
W
Department of Chemistry, Northeastern University, Shenyang, Liaoning, China.
Ge
Xin
X
Wang
Meng
M
Xu
Shukun
S
D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry*; D002417:Cattle; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013154:Spleen / Q000737:chemistry* / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*; D013601:T-Lymphocytes / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry*; D046888:Tissue Array Analysis
D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D017879:Isothiocyanates; D012710:Serum Albumin, Bovine; C037152:isothiocyanic acid; D005978:Glutathione; D013691:Tellurium; C028337:cadmium telluride