[Determination of azaperone and carazolol residues in animals kidney using LC-MS/MS method].
The method is presented to analyze azaperone and carazolol in pigs kidney. Samples were extracted with acetonitryle. The determination was performed by LC-ESI-MS/MS. The LC was equipped with column Luna C18 Phenomenex. Haloperidol was used as internal standards. The method was validation according to the criteria of Decision Commission No 2002/657/EC. Recoveries for the level 100 microg/kg azaperone and 25 microg/kg carazolol were in the range 91.2-107.0% and 70.8-93.2%. The limit of decision (CCalpha) and detection capability (CCbeta) was respectively 125.9 microg/kg, 160.0 microg/kg for azaperone and 29.3 microg/kg, 33.3 microg/kg. LC-MS/MS technique fulfill the requirements of Decision Commission No 2002/657/EC.
Zawadzka
Iwona
I
Pracownia Badań Chemicznych Srodków Spozywczych, Zakład Higieny Weterynaryjnej, Wojewódzki Inspektorat Weterynarii, Białystok. zawadzka@wiw.bianet.com.pl
Rodziewicz
Lech
L
D000818:Animals; D014150:Antipsychotic Agents / Q000032:analysis*; D001376:Azaperone / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D007668:Kidney / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D011412:Propanolamines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine
D014150:Antipsychotic Agents; D011412:Propanolamines; D001376:Azaperone; C014382:carazolol