title: Fabrication of porous extracellular matrix scaffolds from human adipose tissue.
abstract: Adipose tissue is found over the whole body and easily obtained in large quantities with minimal risk by a common surgical operation, liposuction. Although liposuction was originally intended for the removal of undesired adipose tissue, it may provide an ideal material for tissue engineering scaffolds. Here we present novel, porous scaffolds prepared from human adipose tissues. The scaffolds were fabricated in a variety of macroscopic shapes such as round dishes, squares, hollow tubes, and beads. The microscopic inner porous structure was controlled by the freezing temperature, with a decrease in pore size as the freezing temperature decreased. The scaffold prepared from human adipose tissue contains extracellular matrix components including collagen. Preliminary in vitro studies showed that human adipose-derived stem cells attached to a human extracellular matrix scaffold and proliferated. This scaffold based on human adipose tissue holds great promise for many clinical applications in regenerative medicine, particularly in patients requiring soft-tissue regeneration.
authors: Department of Chemical Engineering and Bionanotechnology, Hanyang University, Ansan, Republic of Korea .
authors: Ji Suk
authors: JS
authors: Choi
authors: Hyun-Jin
authors: HJ
authors: Yang
authors: Beob Soo
authors: BS
authors: Kim
authors: Jae Dong
authors: JD
authors: Kim
authors: Sang Hoon
authors: SH
authors: Lee
authors: Eun Kyu
authors: EK
authors: Lee
authors: Kinam
authors: K
authors: Park
authors: Yong Woo
authors: YW
authors: Cho
authors: Hee Young
authors: HY
authors: Lee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000328:Adult; D001483:Base Sequence; D001672:Biocompatible Materials; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D017931:DNA Primers; D005109:Extracellular Matrix; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D017931:DNA Primers; D003094:Collagen