Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tü117.
Analysis of the alpha-lipomycin biosynthesis gene cluster of Streptomyces aureofaciens Tü117 led to the identification of five putative regulatory genes, which are congregated into a subcluster. Analysis of the lipReg1-4 and lipX1 showed that they encode components of two-component signal transduction systems (LipReg1 and LipReg2), multiple antibiotics resistance-type regulator (LipReg3), large ATP-binding regulators of the LuxR family-type regulator (LipReg4), and small ribonuclease (LipRegX1), respectively. A combination of targeted gene disruptions, complementation experiments, lipomycin production studies, and gene expression analysis via RT-PCR suggests that all regulatory lip genes are involved in alpha-lipomycin production. On the basis of the obtained data, we propose that LipReg2 controls the activity of LipReg1, which in its turn govern the expression of the alpha-lipomycin pathway-specific regulatory gene lipReg4. The ribonuclease gene lipX1 and the transporter regulator lipReg3 appear to work independently of genes lipReg1, lipReg2, and lipReg4.
Horbal
Liliya
L
Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, L'viv, Ukraine.
Rebets
Yuriy
Y
Rabyk
Maria
M
Luzhetskyy
Andriy
A
Ostash
Bogdan
B
Welle
Elisabeth
E
Nakamura
Tatsunosuke
T
Fedorenko
Victor
V
Bechthold
Andreas
A
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D006027:Glycosides / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D005810:Multigene Family; D016296:Mutagenesis; D011090:Polyenes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013304:Streptomyces aureofaciens / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D006027:Glycosides; D011090:Polyenes; C548829:alpha-lipomycin