Differential response of Leydig cells in expressing 11beta-HSD type I and cytochrome P450 aromatase in male rats subjected to corticosterone deficiency.
Emerging evidence suggests that the glucocorticoid and estradiol are important for Leydig cell steroidogenesis and are regulated via aromatase for estradiol production and 11beta-HSD for oxidatively inactivating glucocorticoid. Although it is known that corticosterone deficiency impaired Leydig cell steroidogenesis, its effect on the expression of Leydig cell 11beta-HSD type I and aromatase are yet to be recognized. Following metyrapone-induced corticosterone deficiency, serum corticosterone and testosterone levels decrease, whereas serum estradiol remains unaltered. 11beta-HSD type I mRNA and its activity was decreased by corticosterone deficiency, whereas the activity and mRNA of aromatase remains unaltered. Simultaneous administration of corticosterone prevented its deficiency-induced changes of 11beta-HSD type I in Leydig cells. Our results show that metyrapone-induced corticosterone deficiency impairs Leydig cell 11beta-HSD enzyme activity and 11beta-HSD type I mRNA expression, and the Leydig cells need to maintain their intracellular concentration of corticosterone for a normal function.
Parthasarathy
Chandrakesan
C
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, Tamil Nadu, India.
Yuvaraj
Sambandam
S
Ilangovan
Ramachandran
R
Janani
Panneerselvam
P
Kanagaraj
Palaniyandi
P
Balaganesh
Muthusamy
M
Natarajan
Bhaskaran
B
Sittadjody
Sivanandane
S
Balasubramanian
Karundevi
K
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000378:metabolism*; D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood / Q000172:deficiency* / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood
D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D001141:Aromatase; D003345:Corticosterone; D008797:Metyrapone