title: Subcellular localization of the carbohydrate Lewis(x) adhesion structure in hippocampus cell cultures.
abstract: The Lewis(x) (Le(x)) epitope (Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc-R) has been associated with the development of the central nervous system of diverse species including human and rodents. In this work, Le(x) has been found in the tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) compartment of rat hippocampus neurons in culture, at 7 days in vitro (DIV), when neurite extension is abundant. The TI-VAMP compartment is known to be associated with neurite outgrowth. Le(x) was found predominantly in neurites but also in somata and in growth cones. Abundant Le(x)-carrier glycoproteins specific to neurons have been identified at this stage of differentiation. At a later stage of differentiation, at 14 DIV, Le(x) appeared in extrasynaptic sites of GABAergic neurons, and in synaptic sites of glutamatergic neurons.
authors: Instituto de Tecnologia Química e Biológica, Avenida da República, Apartado 127, 2781-901 Oeiras, Portugal.
authors: Catarina
authors: C
authors: Brito
authors: Lydia
authors: L
authors: Danglot
authors: Thierry
authors: T
authors: Galli
authors: Júlia
authors: J
authors: Costa
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002451:Cell Compartmentation / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D006624:Hippocampus / Q000737:chemistry* / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D007983:Lewis Blood Group Antigens / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D016256:Lewis X Antigen / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D016501:Neurites / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D050683:R-SNARE Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000080506:Sialyl Lewis X Antigen; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D013744:Tetanus Toxin / Q000378:metabolism / Q000633:toxicity
chemical_list: D006023:Glycoproteins; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; D050683:R-SNARE Proteins; D000080506:Sialyl Lewis X Antigen; D013744:Tetanus Toxin