title: Non-ischaemic titrated cardiac injury caused by radiofrequency catheter ablation of atrial fibrillation mobilizes CD34-positive mononuclear cells by non-stromal cell-derived factor-1alpha mechanism.
abstract: OBJECTIVE
  It has been known that myocardial ischaemia mobilizes CD34+ bone marrow-derived cells by the stromal cell-derived factor (SDF)-1alpha pathway. We hypothesized that non-ischaemic titrated cardiac injury caused by radiofrequency catheter ablation (RFCA) of atrial fibrillation (AF) recruits CD34+ cells by an alternative mechanism.
  
  
  RESULTS
  Fifty-six patients (39 males, 53.0 +/- 13.5 years old) who underwent electrophysiology study (EPS; n = 10) or RFCA of AF (n = 46) were included. Peripheral blood CD34+ cell count and multiple serologic markers were evaluated before, immediately after, at 24 h, and 10 days after the procedure. The results are as follows: (i) the per cent increase in CD34+ cells (%DeltaCD34+) was significant after RFCA compared with after EPS (P < 0.01), and correlated with RF duration and troponin I, respectively. (ii) In contrast, SDF-1alpha decreased after RFCA and had no correlation with %DeltaCD34+ cells while matrix metalloproteinase (MMP)-9 (P < 0.0001) and GRObeta (P < 0.001) increased after RFCA and had correlations with 24 h %DeltaCD34+ cells.
  
  
  CONCLUSIONS
  Non-ischaemic titrated cardiac injury caused by AF ablation mobilizes CD34+ cells to the peripheral blood through a non-SDF-1alpha pathway associated with MMP-9 and GRObeta.
authors: Korea University Cardiovascular Center, Seoul, Republic of Korea.
authors: Sook Kyoung
authors: SK
authors: Kim
authors: Hui-Nam
authors: HN
authors: Pak
authors: Jae Hyung
authors: JH
authors: Park
authors: Jong Il
authors: JI
authors: Choi
authors: Myung-Hyun
authors: MH
authors: Nam
authors: Yunjung
authors: Y
authors: Jo
authors: Young-Hoon
authors: YH
authors: Kim
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology*; D001281:Atrial Fibrillation / Q000276:immunology* / Q000601:surgery*; D017115:Catheter Ablation / Q000009:adverse effects*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000276:immunology; D054426:Chemokine CXCL2 / Q000276:immunology; D005260:Female; D006335:Heart Injuries / Q000209:etiology* / Q000276:immunology*; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000276:immunology; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000209:etiology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018952:Antigens, CD34; C499706:CXCL2 protein, human; D054377:Chemokine CXCL12; D054426:Chemokine CXCL2; D020780:Matrix Metalloproteinase 9