Voltammetric study of gold-supported lipid membranes in the presence of perfluorooctanesulphonic acid.
The effect of perfluorooctanesulphonic acid (PFOS) on lipid membranes was studied using supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bilayer as the model membrane. Phospholipid bilayer was deposited on gold electrode using a combination of the Langmuir-Blodgett and Langmuir-Schaefer (LB/LS) techniques. Electrodes were modified with two different types of membranes: DMPC bilayers initially containing PFOS and pure DMPC bilayers later exposed to the PFOS solutions. Such approach allowed studying both the changes in membrane characteristic imposed by the perfluorinated compound present in the model membrane and the process of its incorporation into the membrane. Studies with anticancer drug doxorubicin revealed that PFOS inhibits drug transport through the phospholipid bilayer and its effect can be compared to that of cholesterol. Moreover, the different trends observed in the changes in electron transfer rate constant (k(s)) calculated for ferricyanides and in peak current of hexaamineruthenium chloride showed that electrostatic interactions between electroactive probes and PFOS molecules incorporating into phospholipid bilayers play an important role and should be taken into account while explaining the interactions of perfluorooctanesulphonic acid with model biological membranes.
Matyszewska
Dorota
D
Faculty of Chemistry, University of Warsaw, ul. Pasteura 1, 02093 Warsaw, Poland.
Bilewicz
Renata
R
D017738:Alkanesulfonic Acids / Q000737:chemistry* / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D005466:Fluorocarbons / Q000737:chemistry* / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D010539:Permeability / Q000187:drug effects; D012428:Ruthenium / Q000737:chemistry; D013499:Surface Properties
D017738:Alkanesulfonic Acids; D005292:Ferricyanides; D005466:Fluorocarbons; D008051:Lipid Bilayers; C007931:hexacyanoferrate III; D006046:Gold; D012428:Ruthenium; D004317:Doxorubicin; C076994:perfluorooctane sulfonic acid; D004134:Dimyristoylphosphatidylcholine