title: The effects of normoxic versus hyperoxic cardiopulmonary bypass on oxidative stress and inflammatory response in cyanotic pediatric patients undergoing open cardiac surgery: a randomized controlled trial.
abstract: OBJECTIVE
  This study investigates the effects of controlled reoxygenation cardiopulmonary bypass on oxidative stress, inflammatory response, and organ function in children undergoing repair of cyanotic congenital heart defects.
  
  
  METHODS
  Sixty-seven cyanotic patients (median age 15 months, interquartile range 6-49 months) undergoing corrective cardiac surgery were randomized to receive either controlled normoxic (50-0 mm Hg; n = 35) or hyperoxic (150-180 mm Hg; n = 32) cardiopulmonary bypass. Troponin I and 8-isoprostane, C3a, interleukins 6, 8, and 10, cortisol, protein S100, and alpha-glutamate transferase were measured preoperatively and 10 and 30 minutes after starting bypass, on removal of the aortic crossclamp, and 12 and 24 hours thereafter.
  
  
  RESULTS
  Overall, troponin I and 8-isoprostane levels were lower in the controlled normoxic group (-29%, 95% CI -48% to -3%, P = .03, and -26%, 95% CI -44% to -2%, P = .03, respectively). Protein S100 release was also lower in the normoxic group 10 minutes after starting bypass (-26%, 95% CI -40% to -9%, P = .005) and 10 minutes after aortic crossclamp removal (-23%, 95% CI -38% to -3%, P = .02, respectively), but similar at other time points in the two groups (P >or= .17). The alpha-glutamate transferase release was significantly lower in the normoxic group 10 minutes after aortic crossclamp removal (-28%, 95% CI -44% to -9%, P = .006, respectively) but was similar at other times (P >or= .11). Release of C3a, interleukins 6, 8, and 10, and cortisol was similar in the two groups throughout (P >or= .15).
  
  
  CONCLUSIONS
  Controlled reoxygenation on starting cardiopulmonary bypass is associated with reduced myocardial damage, oxidative stress, and cerebral and hepatic injury compared with hyperoxic bypass and similar whole body inflammatory and stress response in cyanotic children undergoing open cardiac surgery.
authors: Bristol Heart Institute, Bristol, United Kingdom. M.Caputo@bris.ac.uk
authors: Massimo
authors: M
authors: Caputo
authors: Amir
authors: A
authors: Mokhtari
authors: Chris A
authors: CA
authors: Rogers
authors: Nayia
authors: N
authors: Panayiotou
authors: Qiang
authors: Q
authors: Chen
authors: Mohamed T
authors: MT
authors: Ghorbel
authors: Gianni D
authors: GD
authors: Angelini
authors: Andrew J
authors: AJ
authors: Parry
mesh_terms: D002315:Cardiopulmonary Bypass / Q000009:adverse effects / Q000379:methods*; D002675:Child, Preschool; D015926:Complement C3a / Q000032:analysis; D003490:Cyanosis; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D005982:Glutathione Transferase; D006330:Heart Defects, Congenital / Q000097:blood / Q000601:surgery*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007223:Infant; D007249:Inflammation / Q000097:blood; D007378:Interleukins / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000097:blood; D009418:S100 Proteins / Q000097:blood; D019210:Troponin I / Q000097:blood
chemical_list: D007378:Interleukins; D009418:S100 Proteins; D019210:Troponin I; C075750:8-epi-prostaglandin F2alpha; D015926:Complement C3a; D015237:Dinoprost; D005982:Glutathione Transferase; D010100:Oxygen; D006854:Hydrocortisone