Immobilization of Candida rugosa lipase on sporopollenin from Lycopodium clavatum.
Sporopollenin is a natural polymer obtained from Lycopodium clavatum, which is highly stable with constant chemical structure and has high resistant capacity to chemical attack. In this study, immobilization of lipase from Candida rugosa (CRL) on sporopollenin by adsorption method is reported for the first time. Besides this, the enzyme adsorption capacity, activity and thermal stability of immobilized enzyme have also been investigated. It has been observed that under the optimum conditions (Spo-E((0.3))), the specific activity of the immobilized lipase on the sporopollenin by adsorption was 16.3U/mg protein, which is 0.46 times less than that of the free lipase (35.6U/mg protein). The pH and temperature of immobilized enzyme were optimized, which were 6.0 and 40 degrees C respectively. Kinetic parameters V(max) and K(m) were also determined for the immobilized lipase. It was observed that there is an increase of the K(m) value (7.54mM) and a decrease of the V(max) value (145.0U/mg-protein) comparing with that of the free lipase.
Tutar
Havva
H
Department of Chemical Engineering, Selcuk University, Campus, 42031 Konya, Turkey.
Yilmaz
Elif
E
Pehlivan
Erol
E
Yilmaz
Mustafa
M
D000327:Adsorption; D001704:Biopolymers / Q000378:metabolism*; D002175:Candida / Q000201:enzymology; D002338:Carotenoids / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000378:metabolism* / Q000648:ultrastructure; D032502:Lycopodium / Q000737:chemistry*; D013696:Temperature
D001704:Biopolymers; D004800:Enzymes, Immobilized; C009800:sporopollenin; D002338:Carotenoids; D008049:Lipase