title: Using human sera to identify a 52-kDa exoantigen of Penicillium chrysogenum and implications of polyphasic taxonomy of anamorphic ascomycetes in the study of antigenic proteins.
abstract: We are interested in isolating and identifying antigenic fungal proteins from species that grow on damp building materials. The indoor clade of Penicillium chrysogenum, the so-called Fleming clade, is the most common species of Penicillium on moldy building materials. We have identified a 52-kDa marker protein for the indoor clade of P. chrysogenum not present in a taxonomically diverse selection of fungi. It is found in high concentrations in protein extracted from the fungus grown on paper-faced gypsum wallboard. During this process, we illuminated the variability in response to patient sera and of strains of the fungus collected over a wide geographic area. From a collection of sera from all over the USA, 25 of the 48 patients reacted to the 52-kDa protein from this prescreened collection of sera. Most strain/antibody combinations had proportionate ELISA response associated with the presence of the target. However, approximately 25% of the strain/patient serum combinations included people who responded to many common allergens from the Penicillia. All the P. chrysogenum strains tested produced the target protein. However, there was considerable variability in patient IgG response to 32-, 30-, and 18-kDa antigens and in their production by the various clade 4 strains. The target protein was not found in spores or culture extracts of a wide selection of relevant fungi. It appears that the previous studies have been conducted on strains of the fungus from the three clades not those associated with the built environment.
authors: Ottawa-Carleton Institute of Chemistry, Carleton University, Steacie Building, Ottawa, ON, K1S 5B6, Canada.
authors: Aaron M
authors: AM
authors: Wilson
authors: Wen
authors: W
authors: Luo
authors: J David
authors: JD
authors: Miller
mesh_terms: D000946:Antigens, Fungal / Q000276:immunology*; D001203:Ascomycota / Q000145:classification* / Q000276:immunology; D003252:Construction Materials / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000276:immunology*; D006801:Humans; D010408:Penicillium chrysogenum / Q000145:classification / Q000276:immunology*
chemical_list: D000946:Antigens, Fungal; D005656:Fungal Proteins