Insulin-like growth factor-binding protein-3 in osteosarcomas and normal bone tissues.
BACKGROUND
To better understand the significance of local insulin-like growth factor-binding protein 3 (IGFBP-3) in the development of osteosarcoma, IGFBP-3 levels and subcellular localization were compared in biopsies from osteosarcomas and unaffected normal bone tissues.


METHODS
IGFBP-3 levels were analyzed by immunohistochemistry in 21 osteosarcomas and 5 unaffected bone tissues. IGFBP-3 levels were compared for patient outcome.


RESULTS
Mature osteocytes of normal bone tissues contained high levels of predominantly nuclear IGFBP-3, whereas only 50% of osteosarcomas contained IGFBP-3-positive tumor cells, with predominantly cytoplasmic IGFBP-3 staining. Comparison of IGFBP-3 levels for patient outcome resulted in two groups. Patients with a low level of IGFBP-3 in osteosarcoma experienced a trend for a shorter survival time than did patients with a high level of IGFBP-3.


CONCLUSIONS
Our results suggest that the levels and subcellular localization of local IGFBP-3 play a role in osteosarcoma development. Further prospective evaluation with higher clinical sample numbers might reveal a prognostic role for IGFBP-3 level in local tumors in patients with osteosarcoma.
Ressler
Sigrun
S
Cell Metabolism and Differentiation Research Group, Institute for Biomedical Aging Research of the Austrian Academy of Sciences, Innsbruck, Austria.
Radhi
Jasim
J
Aigner
Thomas
T
Loo
Christine
C
Zwerschke
Werner
W
Sergi
Consolato
C
D000328:Adult; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D001842:Bone and Bones / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000378:metabolism*; D008297:Male; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology
D018972:Insulin-Like Growth Factor Binding Protein 3