title: Autoantigens in vitiligo identified by the serological selection of a phage-displayed melanocyte cDNA expression library.
abstract: Vitiligo is an acquired idiopathic hypomelanotic disorder characterized by circumscribed depigmented macules resulting from the loss of cutaneous melanocytes. Although the exact cause of vitiligo remains obscure, autoimmunity may play a role in the development of the disease. The present study was undertaken to investigate the applicability of phage display technology to identify B-cell autoantigens in vitiligo. A melanocyte cDNA phage display library was subjected to rounds of enrichment with vitiligo patient IgG. Subsequently, enriched IgG-binding peptides representing putative autoantigens were identified by sequencing their encoding cDNAs. Radioimmunoassays were used to confirm the immunoreactivity of vitiligo patient (n=61) and control (n=28) sera to several of the putative autoantigens. Non-segmental vitiligo patient sera (n=53) were positive for antibody (Ab) reactivity to gamma-enolase (8%); alpha-enolase (9%); heat-shock protein 90 (13%); osteopontin (4%); ubiquitin-conjugating enzyme (15%); translation-initiation factor 2 (6%); and GTP-binding protein, Rab38 (15%). Ab reactivity to at least one of the previously unknown autoantigens was detected in 51% of patients with non-segmental vitiligo. In contrast, Ab reactivity in a group of patients with segmental vitiligo (n=8) was not demonstrated. Overall, the study indicated that the targets of autoantibodies in vitiligo patients can be revealed by employing the methodology of phage display.
authors: Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield, UK.
authors: Elizabeth A
authors: EA
authors: Waterman
authors: David J
authors: DJ
authors: Gawkrodger
authors: Philip F
authors: PF
authors: Watson
authors: Anthony P
authors: AP
authors: Weetman
authors: E Helen
authors: EH
authors: Kemp
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001324:Autoantigens / Q000235:genetics* / Q000276:immunology; D015551:Autoimmunity / Q000235:genetics* / Q000276:immunology; D005260:Female; D015723:Gene Library; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008544:Melanocytes / Q000276:immunology / Q000502:physiology*; D008875:Middle Aged; D019151:Peptide Library; D011863:Radioimmunoassay; D014820:Vitiligo / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: D001324:Autoantigens; D007074:Immunoglobulin G; D019151:Peptide Library