Development of ultra fast liquid chromatographic method for simultaneous determination of nitrendipine and carvone in skin diffusate samples.
A simple and sensitive reverse phase ultra fast liquid chromatographic (UFLC) method for simultaneous determination of nitrendipine and carvone in skin diffusate samples and microemulsions was developed and validated. The separation was achieved using a gradient mobile phase, on an Onyx column. The eluents were monitored by photodiode array detection. The linearity ranges of proposed method were 0.125-50 microg mL(-1) and 0.125-30 microg mL(-1) for nitrendipine and carvone respectively. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 10%. The method was found to be precise, accurate, and specific during the study. The method was successfully applied for simultaneous estimation of nitrendipine and carvone in ex vivo skin diffusate samples and microemulsions.
Gannu
Ramesh
R
National Facilities in Engineering and Technology with Industrial Collaboration (NAFETIC) Centre, University College of Pharmaceutical Sciences, Kakatiya University, Warangal-506 009, Andhra Pradesh, India.
Yamsani
Vamshi Vishnu
VV
Yamsani
Shravan Kumar
SK
Palem
Chinna Reddy
CR
Voruganti
Swathi
S
Yamsani
Madhusudan Rao
MR
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000493:pharmacokinetics; D002121:Calcium Channel Blockers / Q000032:analysis / Q000493:pharmacokinetics; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D000081005:Cyclohexane Monoterpenes; D016903:Drug Monitoring / Q000379:methods*; D004655:Emulsions; D039821:Monoterpenes / Q000032:analysis* / Q000493:pharmacokinetics; D009568:Nitrendipine / Q000032:analysis* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012867:Skin / Q000187:drug effects / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D002121:Calcium Channel Blockers; D000081005:Cyclohexane Monoterpenes; D004655:Emulsions; D039821:Monoterpenes; C006923:carvone; D009568:Nitrendipine