Interleukin-18 gene promoter polymorphism and the risk of nasopharyngeal carcinoma in a Chinese population.
Nasopharyngeal carcinoma (NPC) is one of the most common cancers in southern China. In addition to environmental factors such as Epstein-Barr virus infection and chemical carcinogen exposure, genetic susceptibility has been reported to play a key role in the development of this disease. Interleukin-18 (IL-18) is a multifunctional cytokine that induces interferon-gamma secretion and plays an important role in antitumor immunity. Variations in the DNA sequence of the IL-18 gene promoter may lead to altered IL-18 production and/or activity, so this can modulate an individual's susceptibility to NPC. To test this hypothesis, we analyzed two single-nucleotide polymorphisms of IL-18 gene promoter, -137 G/C and -607 C/A, in 250 patients with NPC and 270 age- and sex-matched controls, using polymerase chain reaction-restriction fragment length polymorphism. Two polymorphisms, -137 G/C and -607 C/A, were in strong linkage disequilibrium. There were significant differences in the genotype and allele distribution of -137 G/C polymorphism of the IL-18 gene among cases and controls. The -137 GC and CC genotypes were associated with a significantly increased risk of NPC as compared with the -137 GG genotypes (odds ratio [OR] = 1.697; 95% confidence interval [CI], 1.158-2.488; p = 0.007, and OR = 2.700; 95% CI, 1.268-5.751; p = 0.008, respectively). Consistent with the results of the genotyping analyses, the -137 C/-607 A haplotype was associated with a significantly increased risk of NPC as compared with the -137 G/-607 C haplotype (OR = 1.721; 95% CI, 1.262-2.349; p = 0.001).
Nong
Le-Gen
LG
Department of Medical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China. nonglegen@gmail.com
Luo
Bin
B
Zhang
Liang
L
Nong
Hong-Bing
HB
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors
D017931:DNA Primers; D020382:Interleukin-18