Detection of cell death in Drosophila.
Drosophila is a powerful model system for the identification of cell death genes and understanding the role of cell death in development. In this chapter, we describe three methods typically used for the detection of cell death in Drosophila. The TUNEL and acridine orange methods are used to detect dead or dying cells in a variety of tissues. We focus on methods for the embryo and the ovary, but these techniques can be used on other tissues as well. The third method is the detection of genetic interactions by expressing cell death genes in the Drosophila eye.
McCall
Kimberly
K
Department of Biology, Boston University, Boston, MA, USA.
Peterson
Jeanne S
JS
Pritchett
Tracy L
TL
D000818:Animals; D017209:Apoptosis; D016923:Cell Death; D004330:Drosophila / Q000166:cytology* / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D005123:Eye / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017344:Genes, Insect; D010053:Ovary / Q000378:metabolism