title: Grape waste extract obtained by supercritical fluid extraction contains bioactive antioxidant molecules and induces antiproliferative effects in human colon adenocarcinoma cells.
abstract: Grape waste management is one of the main problems of winery industries, but, conversely, grape waste contains a high amount of polyphenols that might protect against human diseases related to oxidative stress, such as colorectal cancer. Therefore, the aim of this work was to investigate the antioxidant and antiproliferative activities of a grape waste extract obtained by supercritical fluid extraction. Because the beneficial effect of grape is related to its content of polyphenolic molecules, the extract was chemically characterized by high-performance liquid chromatography in order to assess its major bioactive components. The antioxidant activity of the grape extract was determined. The results showed that the grape extract presents a strong antiradical activity in the in vitro 2,2-diphenyl-1-picrylhydrazyl radical assay and protects against reactive oxygen species production in human colon adenocarcinoma cells (Caco-2). In contrast, the extract did not protect in the citronellal thermooxidation system and showed a weak protective action against lipid peroxidation in Caco-2 cells. The clonogenic assay and the cell cycle distribution analysis showed that the grape extract has a significant antiproliferative effect in a tumor cell line. These data indicate that grape extract is a promising product to be used as an anti-free radical agent and could exert a chemopreventive action.
authors.affiliation: Dipartimento di Medicina Sperimentale, Università di Pavia , Italy.
authors.forename: Maria Claudia
authors.initials: MC
authors.lastname: Lazzè
authors.forename: Roberto
authors.initials: R
authors.lastname: Pizzala
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Gutiérrez Pecharromán
authors.forename: Paloma
authors.initials: P
authors.lastname: Gatòn Garnica
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Antolín Rodríguez
authors.forename: Nicola
authors.initials: N
authors.lastname: Fabris
authors.forename: Livia
authors.initials: L
authors.lastname: Bianchi
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D005609:Free Radicals / Q000378:metabolism; D005638:Fruit; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*; D018505:Waste Management / Q000379:methods
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D005609:Free Radicals; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; C004931:1,1-diphenyl-2-picrylhydrazyl