Simultaneous determination of oxyresveratrol and resveratrol in rat bile and urine by HPLC after oral administration of Smilax china extract.
Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4'-trihydroxystilbene, RES) are the two most important constituents of the traditional Chinese medicine Smilax china. A reversed-phase high-performance liquid chromatographic method was developed to determine OXY and RES in rat bile and urine after oral administration of Smilax china extract. The biological samples were analyzed by HPLC on Aglient Zorbax SB-C18 column (250 x 4.6 mm, 5 microm) at a wavelength 320 nm and at a flow rate of 1.0 mL/min. The method was accurate and reproducible for determination. The cumulative excretion of OXY and RES was 0.29% and 0.97% in bile samples, 0.84% and 0.65% in urine samples, respectively.
Huang
Hui-lian
HL
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, PR China.
Zhang
Jin-qiang
JQ
Chena
Guang-tong
GT
Lu
Zhi-qiang
ZQ
Sha
Na
N
Guo
De-an
DA
D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D012680:Sensitivity and Specificity; D032281:Smilax / Q000737:chemistry*; D013267:Stilbenes / Q000737:chemistry* / Q000378:metabolism / Q000652:urine
D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D000077185:Resveratrol