title: Temporal changes in expression of aquaporin-3, -4, -5 and -8 in rat brains after permanent focal cerebral ischemia.
abstract: Morbidity and mortality in stroke are attributed to cerebral edema. To date, six aquaporins (AQPs) have been found in rat brains. Whereas studies have been focused on AQP1, -4 and -9, little is known about the expression of AQP3, -5 and -8. To clarify roles of AQP3, -5 and -8 in water movement, we examined the expression patterns of AQP3, -5 and -8 in ischemic brains from the rats with permanent middle cerebral artery occlusion (pMCAO). We also investigated the expression of AQP4 after ischemia, which was used as a positive control. We found that the expression of AQP4 increased continuously until 24 h after pMCAO in both the ischemic core and the border region. The increased expression was correlated with brain swelling, whereas the expression of AQP3, -5 and -8 continued to increase until 24 h after pMCAO in the border region but decreased 6 h after pMCAO in the ischemic core. We also found AQPs were colocalized with GFAP-positive astrocytes and/or NeuN-positive neurons in rat brains. This is the first study describing the expression of AQP3, -5 and -8 in rat brains subjected to pMCAO. Our findings indicated that dynamic changes of AQP3, -4, -5 and -8 expression could contribute to the development of cerebral edema after brain ischemia. Besides, AQP3, -5 and -8 may be involved in the neuronal swelling.
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Wei Hua
authors.initials: WH
authors.lastname: Yu
authors.forename: Shan Quan
authors.initials: SQ
authors.lastname: Sun
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D020346:Aquaporins / Q000378:metabolism*; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western; D001834:Body Water / Q000378:metabolism; D001923:Brain Chemistry; D001929:Brain Edema / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D020346:Aquaporins