[Effect and mechanism of Coeloglossum viride var. bracteatum extract on scopolamine-induced deficits of learning and memory behavior of rodents].
The aim of the present study is to investigate the effect and mechanism of Coeloglossum viride var. bracteatum extract (CE) on scopolamine-induced learning and memory deficits. Learning and memory deficits of mice were evaluated by step-down passive avoidance test. Long-term potentiation of rats was detected in the dentate gyrus of hippocampus. Brain acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) activities were also determined. The results showed that scopolamine impaired learning and memory performance and LTP induction in hippocampus. Oral administration of CE (5, 10, and 20 mg x kg(-1)) significantly alleviated scopolamine-induced memory deficits measured by step-down test (P < 0.05). CE (5 mg x kg(-1), ip) significantly reversed the inhibitory effect of scopolamine on LTP in rats. In addition, CE was found to increase the activity of ChAT in rat brain. These results suggested that CE could alleviate scopolamine-induced learning and memory deficits, which might be due to the LTP-improvement and ChAT activity enhancement.
Li
Min
M
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, China.
Wang
Ya-fang
YF
Ma
Bo
B
Liu
Geng-tao
GT
Zhang
Jian-jun
JJ
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D018891:Dentate Gyrus / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006624:Hippocampus / Q000503:physiopathology; D007858:Learning / Q000187:drug effects*; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology; D051379:Mice; D029595:Orchidaceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012601:Scopolamine; D013386:Succinates / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013386:Succinates; C479293:dactylorhin B; C545907:militarine; D012601:Scopolamine; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase