title: [Anti-tumor effects on human laryngeal carcinoma Hep-2 of recombinant fowlpox virus expressing chicken anemia virus Apoptin gene].
abstract: OBJECTIVE
  To investigate the anti-tumor effects and the mechanism of the recombinant fowlpox virus expressing Apoptin gene on human laryngeal carcinoma Hep-2.
  
  
  METHODS
  Hep-2 cells cultured in vitro were infected with vFVApoptin. The anti-tumor effects on Hep-2 cells were measured through MTT staining and, the mitochondrial trans-membrane potential (delta psi m) and reactive oxygen species (ROS) were analyzed by flow cytometry. Western blot was used to detect the release of cytochrome c (Cyto c). Caspase-3/9 activities were measured by colorimetric assay.
  
  
  RESULTS
  vFVApoptin could restrain Hep-2 cells significantly and, had the function of down-regulating delta psi m, up-regulating ROS, promoting Cyto c release and activating Caspase-3/9.
  
  
  CONCLUSIONS
  Cyto c were released from mitochondria by the function of up-regulating ROS of vFVApoptin. Cyto c triggered Caspase-9 and, after the activation of Caspase-9, downstream apoptotic factors, such as caspase-3, were activated. Eventually, Hep-2 cells were suppressed by mitochondrial pathway apoptosis induced by vFVApoptin.
authors.affiliation: Department of Otorhinolaryngology, the Second Hospital, Bethune Faculty of Medical Sciences of Jilin University, Changchun, 130041, China. guan-guo@163.com
authors.forename: Guofang
authors.initials: G
authors.lastname: Guan
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.forename: Chunshun
authors.initials: C
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Lou
authors.forename: Ping
authors.initials: P
authors.lastname: Shi
authors.forename: Yanru
authors.initials: Y
authors.lastname: Hao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D036022:Capsid Proteins / Q000235:genetics / Q000494:pharmacology*; D017727:Chicken anemia virus / Q000235:genetics; D005587:Fowlpox virus / Q000235:genetics; D006801:Humans; D014407:Tumor Cells, Cultured
chemical_list: D036022:Capsid Proteins; C091717:VP3 protein, Chicken anemia virus