title: Human peritoneal membrane reduces the formation of intra-abdominal adhesions in ventral hernia repair: experimental study in a chronic hernia rat model.
abstract: BACKGROUND
  Adhesions leading to intestinal obstructions and fistulae are severe complications related to the intraperitoneal placement of synthetic meshes. This study evaluated the efficacy of human peritoneal membrane (HPM) in a chronic hernia repair rat model as an anti-adhesive solution for preventing the development of intra-abdominal adhesions.
  
  
  METHODS
  The mechanical properties of HPM and human fascia lata (HFL) were evaluated prior to in vivo implantation. Twenty rats underwent midline laparotomy, which led to the development of chronic hernias 28 d later. Then, animals underwent incisional hernia repair in an underlay fashion (n=5/mesh group) with compressed poly(tetra-fluoro-ethylene) (cPTFE), onto which HPM or HFL were affixed pre-repair, along with two additional controls. The extent and tenacity of intra-abdominal adhesions were determined through qualitative gross evaluations and quantitative tensiometry at 30 d post-repair. The host tissue response was evaluated histologically.
  
  
  RESULTS
  In hydrated state, the elastic properties of HPM were superior to HFL. Repairs with HPM had significantly less surface area covered by adhesions, with significantly lower tenacity compared with all other groups. Furthermore, intra-abdominal adhesions developed in the presence of HPM were associated with omentum only, and were distributed around the perimeter of the exposed cPTFE. HPM served as an active tissue remodeling template, replacing the traditional foreign body encapsulation with an anatomically and physiologically superior outcome.
  
  
  CONCLUSIONS
  HPM significantly reduces the extent and tenacity of intra-abdominal adhesion formation, and represents a bioprosthetic template that encourages structural and functional neo peritonealization.
authors.affiliation: Proxy Biomedical Limited, Spiddal Co., Galway, Ireland. gabriela.voskerician@proxybiomedical.com
authors.forename: Gabriela
authors.initials: G
authors.lastname: Voskerician
authors.forename: Judy
authors.initials: J
authors.lastname: Jin
authors.forename: Shawn A
authors.initials: SA
authors.lastname: Hunter
authors.forename: Christina P
authors.initials: CP
authors.lastname: Williams
authors.forename: Michael
authors.initials: M
authors.lastname: White
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Rosen
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D006555:Hernia, Ventral / Q000601:surgery*; D006801:Humans; D010537:Peritoneum / Q000637:transplantation*; D010538:Peritonitis / Q000473:pathology / Q000517:prevention & control*; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013526:Surgical Mesh; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*; D014183:Transplantation, Heterologous