Size distribution of native cytosolic proteins of Thermoplasma acidophilum.
We used molecular sieve chromatography in combination with LC-MS/MS to identify protein complexes that can serve as templates in the template matching procedures of visual proteomics approaches. By this method the sample complexity was lowered sufficiently to identify 464 proteins and - on the basis of size distribution and bioinformatics analysis - 189 of them could be assigned as subunits of macromolecular complexes over the size of 300 kDa. From these we purified six stable complexes of Thermoplasma acidophilum whose size and subunit composition - analyzed by electron microscopy and MALDI-TOF-MS, respectively - verified the accuracy of our method.
Sun
Na
N
Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany.
Tamura
Noriko
N
Tamura
Tomohiro
T
Knispel
Roland Wilhelm
RW
Hrabe
Thomas
T
Kofler
Christine
C
Nickell
Stephan
S
Nagy
István
I
D019843:Archaeal Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D003600:Cytosol / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D013822:Thermoplasma / Q000378:metabolism*
D019843:Archaeal Proteins