title: Dynamics of ATP and movement in Eurasian perch (Perca fluviatilis L.) sperm in conditions of decreasing osmolality.
abstract: Repetitive activation of perch (Perca fluviatilis L.) sperm motility was investigated in this study. The first phase of sperm motility activation was initiated by dilution in a 260 mM glucose solution (75% motility). The second phase of motility was achieved by adding water to previously activated sperm, so that the glucose concentration dropped to 220 mM (24% motility). Finally, the third phase was obtained by further addition of water (down to 90 mM glucose) to the activated sperm suspension (15% motility). Parallel measurements of sperm ATP content were also made. The median value for nonactivated sperm was 43.9 nmol ATP/10(9) spermatozoa. The ATP concentration decreased significantly from 35 to 7 nmol ATP/10(9) spermatozoa after successive activations of motility in the above glucose solutions. Sperm velocity ranged in value from 25 to 330 microm/sec at 10 sec postactivation, from 10 to 290 microm/sec at 30 sec, and from 0 to 200 microm/sec at 45 sec. A model postulating several classes in the population of spermatozoa is developed, tentatively accounting for such successive activation. Possible further application of multiple sperm activation is discussed.
authors.affiliation: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic. boryss00@vurh.jcu.cz
authors.forename: S
authors.initials: S
authors.lastname: Boryshpolets
authors.forename: B
authors.initials: B
authors.lastname: Dzyuba
authors.forename: V
authors.initials: V
authors.lastname: Stejskal
authors.forename: O
authors.initials: O
authors.lastname: Linhart
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D008297:Male; D009997:Osmotic Pressure; D010471:Perches; D010865:Pilot Projects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
chemical_list: D003470:Culture Media; D000255:Adenosine Triphosphate; D005947:Glucose