Simultaneous quantification of eight major bioactive phenolic compounds in Chinese propolis by high-performance liquid chromatography.
A simple, sensitive and specific high-performance liquid chromatography-UV (HPLC-UV) method has been developed to simultaneously quantify the eight major bioactive phenolic compounds in Chinese propolis, namely caffeic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin 5-methyl ether, pinocembrin, benzyl caffeate, chrysin and galangin. This HPLC assay was performed on an Agilent Zorbax Extend-C18 (250 x 4.6 mm, 5 microm) column with a gradient of methanol and 0.2% aqueous acetic acid (v/v) in 50 min, at a flow rate of 1.0 mL/min, and detected at 290 nm. All calibration curves showed good linearity (r2 > 0.999) within the test ranges. The intra- and inter-day assay precision (RSD) of eight phenolic compounds were in the range of 0.07-4.92%. The recoveries were between 98.3% and 104.8%. This assay was applied to the evaluation of nineteen samples from different origins in China. The results indicated that the developed assay could be readily utilized for the quality control of propolis.
Sha
Na
N
College of Traditional Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Huang
Hui-Lian
HL
Zhang
Jin-Qiang
JQ
Chen
Guang-Tong
GT
Tao
Si-Jia
SJ
Yang
Min
M
Li
Xing-Nuo
XN
Li
Ping
P
Guo
De-An
DA
D002681:China; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry*
D010636:Phenols; D011429:Propolis