NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42.
Cdc42(T35A) is an active construct of Cdc42, a Ras GTPase involved in signal transduction, containing a single-point mutation in an important effector-binding region. We determined the backbone and side chain resonance assignments of (13)C,(15)N-labelled Cdc42(T35A) from E. coli.
Adams
Paul D
PD
Department of Chemistry and Biochemistry, University of Arkansas-Fayetteville, 72701, USA. pxa001@uark.edu
Oswald
Robert E
RE
D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008970:Molecular Weight; D009154:Mutation; D009587:Nitrogen Isotopes / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011522:Protons; D020764:cdc42 GTP-Binding Protein / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D020764:cdc42 GTP-Binding Protein