[Modification and application of the micropipette aspiration method for measuring the cell adhesion force on material surface].
The micropipette aspiration technique was improved by selecting the uniform cells in round shape (UCR) from a number of adhered cells on the testing surface so as to measure the adhesion forces during the initial adhesion stage. Silk fibroin film and silk fibroin/fucoidan film were used as testing substrates. The testing results revealed that the modified micropipette method was more rapid for testing. The tendency of cell adhesion force on different materials surface under different conditions remained unchanged. The testing results showed good reproducibility. It was easy to distinguish small differences in the adhesiveness of endothelial cell on the silk fibroin based materials, thus exhibiting the high sensitivity of the improved method.
Gao
Zhen
Z
Research Center of Material Science, Beijing Institute of Technology, Beijing 100081, China.
Wang
Song
S
Zhu
Hesun
H
Xu
Jiachao
J
D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D005349:Fibroins; D006801:Humans; D014471:Umbilical Veins / Q000166:cytology
C482288:fibroin, silkworm; D005349:Fibroins