Dissecting the role of integrin subunits alpha 2 and beta 3 in rotavirus cell entry by RNA silencing.
Several cell surface molecules have been implicated in rotavirus cell entry, however, their individual relevance during this process is unknown. In this work, the expression of integrins alpha2, beta2, and alpha v beta 3, the heat shock cognate protein 70, and of ganglioside GM1 in different cell lines of human and simian origin was correlated with the infectivity of four rotavirus strains. We observed that different combinations of receptor expression correlated with the infectivity of rotavirus strains, suggesting that the participation of several receptors is important for rotavirus infection. To characterize the relevance of integrins alpha2 and alpha v beta 3 in more detail, their expression was silenced using RNA interference. About 80% decrease in the cell content of integrins resulted in 15-30% decrease of infectivity of strains RRV and Wa when measured by a focus-forming assay, while there was no decrease of infectivity when measured by flow cytometry in integrin-deficient cells. Altogether these data suggest that integrins alpha2 and alpha v beta 3 do not play a major role in the rotavirus entry process.
Isa
Pavel
P
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, CP 62210 Cuernavaca, Morelos, Mexico. pavel@ibt.unam.mx
Sánchez-Alemán
Miguel Angel
MA
López
Susana
S
Arias
Carlos F
CF
D000818:Animals; D002460:Cell Line; D005677:G(M1) Ganglioside / Q000235:genetics / Q000502:physiology; D055785:Gene Knockdown Techniques / Q000379:methods; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics / Q000502:physiology*; D039302:Integrin alphaVbeta3 / Q000235:genetics / Q000502:physiology; D039661:Integrin beta3 / Q000235:genetics / Q000502:physiology*; D008253:Macaca mulatta; D011991:Receptors, Virus / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D012401:Rotavirus / Q000502:physiology*; D053586:Virus Internalization
D018840:HSP70 Heat-Shock Proteins; D039421:Integrin alpha2; D039302:Integrin alphaVbeta3; D039661:Integrin beta3; D011991:Receptors, Virus; D005677:G(M1) Ganglioside