[Effect of zoledronic acid on the growth and CD138 expression of myeloma cell line KM3].
OBJECTIVE
To study the inhibitory effect of zoledronic acid (ZA) on the growth and CD138 expression of myeloma cell line KM3.


METHODS
KM3 cells were treated with different concentrations of ZA The growth of KM3 cells was measured by trypan blue dye exclusion, and the changes of apoptosis rate, cell cycle and expression of CD138 induced by ZA by flow cytometry.


RESULTS
Within the concentration of 10(-5)-10(-3) mol/L, ZA obviously inhibited the growth of KM3 cells in a dose dependent manner. IBN at 10(-5)-10(-4) moL/L increased Annexin V positive rate, blocked cells at the S/G2 boundary, reduced the expression of CD138 and its fluorescence intensity.


CONCLUSIONS
ZA can inhibit the growth of KM3 cells in a dose-dependent manner and inhibited CD138 expression. The mechanism is probably related to induction cell cycle accumulation in S phase and apoptosis.
Huang
Hong-Ming
HM
Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Ding
Run-Sheng
RS
Xiang
Xi-Xi
XX
Hou
Jian
J
D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004164:Diphosphonates / Q000494:pharmacology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D053668:Syndecan-1 / Q000378:metabolism*; D000077211:Zoledronic Acid
D004164:Diphosphonates; D007093:Imidazoles; C508241:SDC1 protein, human; D053668:Syndecan-1; D000077211:Zoledronic Acid