Consequences of head injury and static cold storage on hepatic function: ex vivo experiments using a model of isolated perfused rat liver.
The purpose of the study was to evaluate the effect of head injury (HI) on the metabolic and energy functions of the liver and its consequences after cold storage. In male SD rats with HI, livers were isolated 4 days after injury and perfused either immediately (HI) or after 24 hours of cold preservation. Livers isolated from healthy rats were treated identically. The hepatic functions were explored with an isolated perfused liver model. Head injury induced a liver atrophy without significant difference in the intrahepatic energy level versus healthy rats. After cold storage, hepatic adenosine triphosphate and glycogen contents in HI rats were similar to those of healthy rats. The livers of the HI group that underwent cold preservation had a lower protein catabolism. The portal flow rate at the time of reperfusion was significantly increased in the HI group. In conclusion, static cold storage of livers harvested from HI rats revealed a net protein catabolism reduction and a modification of hepatic microcirculation.
Levesque
Eric
E
Laboratoire de Biologie de la Nutrition EA 2498 Faculté de Pharmacie Université Paris Descartes, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.
Moinard
Christophe
C
Besson
Valérie
V
Neveux
Nathalie
N
Chaumeil
Jean-Claude
JC
Cynober
Luc
L
Charrueau
Christine
C
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D006259:Craniocerebral Trauma / Q000503:physiopathology*; D015925:Cryopreservation; D004734:Energy Metabolism / Q000502:physiology; D022781:Hepatocytes / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000502:physiology* / Q000503:physiopathology*; D008111:Liver Function Tests; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D019188:Organ Preservation Solutions; D009929:Organ Size / Q000502:physiology; D011169:Portal Vein / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000227:Adenine Nucleotides; D008112:Liver Glycogen; D019188:Organ Preservation Solutions