Antibody limits in vivo murid herpesvirus-4 replication by IgG Fc receptor-dependent functions.
Antibody is an important antiviral defence. However, it is considered to do little against human gamma-herpesviruses, which establish predominantly latent infections regulated by T cells. One limitation on analysing these infections has been that latency is already well-established at clinical presentation; early infection may still be accessible to antibody. Here, using murid herpesvirus-4 (MuHV-4), we tested the impact of adoptively transferred antibody on early gamma-herpesvirus infection. Immune sera and neutralizing and non-neutralizing monoclonal antibodies (mAbs) all reduced acute lytic MuHV-4 replication. The reductions, even by neutralizing mAbs, were largely or completely dependent on host IgG Fc receptors. Therefore, passive antibody can blunt acute gamma-herpesvirus lytic infection, and does this principally by IgG Fc-dependent functions rather than by neutralization.
Wright
Debbie E
DE
Division of Virology, Department of Pathology, University of Cambridge, UK.
Colaco
Susanna
S
Division of Virology, Department of Pathology, University of Cambridge, UK.
Colaco
Camilo
C
Immunobiology Ltd, Babraham Research Campus, Cambridge, UK.
Stevenson
Philip G
PG
Division of Virology, Department of Pathology, University of Cambridge, UK.
D019264:Adoptive Transfer; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009500:Neutralization Tests; D011961:Receptors, Fc / Q000276:immunology*; D018144:Rhadinovirus / Q000276:immunology* / Q000502:physiology*; D044967:Serum / Q000821:virology; D010948:Viral Plaque Assay; D014779:Virus Replication; D051598:Whole Body Imaging
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011961:Receptors, Fc