Surface modification of and selective protein attachment to a flexible microarray pattern using atmospheric plasma with a reactive gas.
The hydrophobic and hydrophilic properties of the surface of poly-ether sulfone (PES) films were controlled by an atmospheric pressure plasma (AP) treatment using reactive gases (Ar/H(2) and Ar/O(2)). The surface properties of the Ar/H(2) and Ar/O(2) in series AP-treated PES films showed higher surface roughness (approximately 120%), surface energy (approximately 30%) and hydrophilic properties (oxygen content approximately 10%) than the Ar/O(2) AP-treated PES film. The protein staining results confirmed that an activated region on the patterned PES film with high selectivity and sensitivity was well-defined and formed. This method is suitable for fabricating flexible protein adhesive chips with uniform biomolecular adhesive properties.
Lee
Hyun-Uk
HU
College of Nanoscience and Nanotechnology, Pusan National University, Busan, Republic of Korea.
Park
So-Young
SY
Kang
Yoon-Hee
YH
Jeong
Se-Young
SY
Choi
Sae-Hae
SH
Jahng
Kwang-Yeop
KY
Cho
Chae-Ryong
CR
D001274:Atmospheric Pressure; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
D011506:Proteins