[Effect of FK506 and CSA on the cell survival of engrafted HSC and the level of IL-2 and IL-4 in leukemia mice].
OBJECTIVE
To explore the effect of FK506 and CSA on the survival of HSCs and the level of IL-2 and IL-4.


METHODS
The WEHI23 cells were injected into vein from tail of mice to set up the leukemia model. The Hematopoietic stem cells (HSCs) were harvested from GFP mice and cultured in vitro, then injected into the vein of leukemia mice. FK506 and CSA, as two immunosuppress agents, were applied in leukemia mice and intact mice, and leukemia mice were as control.


RESULTS
Some HSCs could survive in the host treated with FK506 and CSA at least 2 weeks, while HSCs survived only 1 week in intact or leukemia control mice. Simultaneously, the level of IL-2 and IL-4 in leukemia mice treated with FK506 and CSA decreased significantly than those of intact or leukemia control mice.


CONCLUSIONS
FK506 can promote the survival of HSCs in host, and the mechanism may be associated with the down regulation of IL-2 and IL-4.
Chen
Jing
J
Department of Pediatric, Nan fang Medical University, Guangzhou 510515, China.
Li
Chun-fu
CF
D000818:Animals; D016572:Cyclosporine / Q000627:therapeutic use*; D006085:Graft Survival / Q000187:drug effects*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015479:Leukemia, Myelomonocytic, Acute / Q000378:metabolism / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016559:Tacrolimus / Q000627:therapeutic use*; D014184:Transplantation, Homologous
D007166:Immunosuppressive Agents; D007376:Interleukin-2; D015847:Interleukin-4; D016572:Cyclosporine; D016559:Tacrolimus