title: Electrogenerated chemiluminescence ethanol biosensor based on alcohol dehydrogenase functionalized Ru(bpy)3(2+) doped silica nanoparticles.
abstract: An ethanol biosensor, based on the electrogenerated chemiluminescence of Ru(bpy)(3)(2+)-doped silica nanoparticles (RuSiNPs), was investigated in this study. The biosensor was a modified glassy carbon electrode, where alcohol dehydrogenase was crosslinked to RuSiNPs, and then immobilized on the electrode surface using chitosan. The results indicated that the biosensor exhibited excellent performance during ethanol determination with a wide linear range (10(-7) to 10(-2) M), low detection limit (5.0x10(-8) M) and good stability.
authors.affiliation: Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Tian-tian
authors.initials: TT
authors.lastname: Jia
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Cai
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Huang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Guo-nan
authors.initials: GN
authors.lastname: Chen
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D056831:Coordination Complexes; D004563:Electrochemistry / Q000379:methods; D000431:Ethanol / Q000032:analysis*; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D000426:Alcohol Dehydrogenase; D056831:Coordination Complexes; D000431:Ethanol; D012822:Silicon Dioxide; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds