[Nanocomplexes of recombinant proteins and polysialic acid: preparation, characteristics, and biological activity].
A preparation of nanocomplexes containing recombinant proteins (interferons alpha2b and beta1b, insulin, and human granulocyte colony stimulating factor) and natural polysialic acid (PSA) has been described. The incorporation of protein into the complex changes its electrophoretic mobility. Atomic force microscopy reveals the average size of 23-kD insulin complexes with PSA of 10-20 nm and demonstrates that more than 60% of glycopolymer molecules carry a single protein molecule. Experiments with cultured cells show that cytokines bound to polysialic acid retain their ability to regulate cell proliferation. Insulin bound to PSA has a prolonged hypoglycemic effect in vivo.
Bezuglov
V V
VV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, V-437, Moscow, 117997 Russia. vvbez@oxylipin.ibch.ru
Gretskaya
N M
NM
Klinov
D V
DV
Bobrov
M Iu
MIu
Shibanova
E D
ED
Akimov
M G
MG
Fomina-Ageeva
E V
EV
Zinchenko
G N
GN
Bairamashvili
D I
DI
Miroshnikov
A I
AI
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D007328:Insulin / Q000737:chemistry* / Q000494:pharmacology; D000077190:Interferon alpha-2; D000068576:Interferon beta-1b; D016898:Interferon-alpha / Q000737:chemistry* / Q000494:pharmacology; D016899:Interferon-beta / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D012794:Sialic Acids / Q000737:chemistry* / Q000494:pharmacology
D000970:Antineoplastic Agents; D007004:Hypoglycemic Agents; D007328:Insulin; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D012794:Sialic Acids; C021319:polysialic acid; D016179:Granulocyte Colony-Stimulating Factor; D000068576:Interferon beta-1b; D016899:Interferon-beta