title: Phytosterols accumulation in the seeds of Linum usitatissimum L.
abstract: A comparative study was performed to determine the free sterols content and composition during the development of three varieties of linseed (H52, O116 and P129). Seed samples were collected at regular intervals from 7 to 60 days after flowering (DAF). Ten compounds were identified: cholesterol, campesterol, brassicasterol, stigmasterol, beta-sitosterol, Delta5-avenasterol, cycloartenol; 24-methylene cycloartanol, obtusifoliol, citrostadienol. The maximum level of 4-desmethylsterols (1,515 mg/100g oil) was reached at 7 DAF in P129 variety. H52 had the highest level of 4-4 dimethylsterols (355 mg/100g oil) at 28 DAF. The greatest amount of 4-monomethylsterols (35 mg/100g oil) was detected in H52 at 14 DAF. During linseed development, beta sitosterol (830 mg/100g oil) was the major 4-desmethylsterols, followed by campesterol (564 mg/100g oil) and stigmasterol (265 mg/100g oil). Some of these compounds followed nearly the same accumulation pattern during linseed maturation.
authors.affiliation: Laboratoire de Biochimie des lipides, Département de Biologie, Faculté des sciences de Tunis, 2092 ELmanar-Tunisie, Tunisia. wahid1bio@yahoo.fr
authors.forename: Wahid
authors.initials: W
authors.lastname: Herchi
authors.forename: Saoussem
authors.initials: S
authors.lastname: Harrabi
authors.forename: Khaled
authors.initials: K
authors.lastname: Sebei
authors.forename: Sophie
authors.initials: S
authors.lastname: Rochut
authors.forename: Sadok
authors.initials: S
authors.lastname: Boukhchina
authors.forename: Claude
authors.initials: C
authors.lastname: Pepe
authors.forename: Habib
authors.initials: H
authors.lastname: Kallel
mesh_terms: D002775:Cholestadienols / Q000032:analysis; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis; D002855:Chromatography, Thin Layer; D019597:Flax / Q000737:chemistry* / Q000254:growth & development; D035264:Flowers / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry; D010840:Phytosterols / Q000032:analysis*; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development; D012855:Sitosterols / Q000032:analysis; D013045:Species Specificity; D013265:Stigmasterol / Q000032:analysis; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis
chemical_list: D002775:Cholestadienols; D010840:Phytosterols; D012855:Sitosterols; D014315:Triterpenes; C066041:24-methylene cycloartanol; C003532:brassicasterol; C008693:obtusifoliol; C021273:campesterol; C025473:gamma-sitosterol; D002784:Cholesterol; D013265:Stigmasterol; C100089:cycloartenol