[Determination of 29 pesticide residues in tobacco by gas chromatography-electron impact ionization mass spectrometry].
A method was developed for rapid determination of 29 pesticide residues in tobacco based on gas chromatography coupled with electron impact ionization mass spectrometric detection (GC-EI/MS). After the optimization of different parameters, such as the extraction solvent, elution solvent, purificant, pesticides were extracted from tobacco with hexaneacetone (1: 1, v/v) in an ultrasonic bath, cleaned-up on a column, packed with Florisil and neutral alumina, eluted with dichloromethane-hexane (95:5, v/v), and then determined by GC-EI/MS in the selected ion monitoring mode (SIM) with triphenyl phosphate (TPP) as internal standard. The recoveries were performed at 20, 50 and 100 microg/kg fortification levels for each pesticide, and the recoveries ranged from 70% to 110% with the relative standard deviations between 2% and 8%. The detection limits were less than 0.8 microg/kg for 26 pesticides except for the fenpropathrin, deltamethrin and permethrin. The method was linear over the range of 5.0-500.0 microg/kg with the correlation coefficients between 0.9994 to 0.9999. The method was successfully applied to the analysis of these pesticides in tobacco.
Zou
Ximei
X
Department of Chemistry and the Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Lin
Zhuguang
Z
Peng
Shunü
S
Chen
Zhaobin
Z
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006843:Hydrocarbons, Chlorinated / Q000032:analysis; D009943:Organophosphorus Compounds / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis; D014026:Nicotiana / Q000737:chemistry*
D006843:Hydrocarbons, Chlorinated; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D011722:Pyrethrins