title: The erosive potential of some alcopops using bovine enamel: an in vitro study.
abstract: OBJECTIVE
  Alcoholic soft drinks have become increasingly popular and have high concentrations of citric acid and alcohol so might have the potential to cause dental erosion. This study aimed to investigate the erosive potential of alcopops on bovine enamel in vitro.
  
  
  METHODS
  Six bovine upper incisors were prepared and sectioned to give six slabs per tooth, 4mm x 4mm each. Each slab was covered with nail varnish, leaving an exposed window (2mm x 2mm). Samples were immersed in 20ml of each of the test solutions for 20min, 1h, and 24h under gentle agitation (100rpm). Enamel surface loss was determined using Quantitative Laser Fluorescence (QLF), Non-contact Profilometry (NCP) and Transverse Microradiography (TMR).
  
  
  RESULTS
  Enamel loss occurred with all test drinks and the positive control (p<0.05), and the depth of lesion correlated with pH and time. No significant difference was observed between 20min and 1h exposure, although both times had significantly (p<0.05) greater erosion when compared with baseline. Within each alcopops group significant erosion had occurred at 24h exposure compared with the baseline and previous times.
  
  
  CONCLUSIONS
  All the tested alcopops resulted in significant enamel loss at 24h (p<0.001) with direct correlation between degree of enamel loss and both pH and increasing exposure time.
authors.affiliation: School of Dental Sciences, University of Liverpool, Liverpool, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Ablal
authors.forename: J S
authors.initials: JS
authors.lastname: Kaur
authors.forename: L
authors.initials: L
authors.lastname: Cooper
authors.forename: F D
authors.initials: FD
authors.lastname: Jarad
authors.forename: A
authors.initials: A
authors.lastname: Milosevic
authors.forename: S M
authors.initials: SM
authors.lastname: Higham
authors.forename: A J
authors.initials: AJ
authors.lastname: Preston
mesh_terms: D000434:Alcoholic Beverages / Q000009:adverse effects*; D000818:Animals; D002253:Carbonated Beverages / Q000009:adverse effects*; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D008852:Microradiography; D014077:Tooth Erosion / Q000139:chemically induced*