Induction of apoptosis by immature plum in human hepatocellular carcinoma.
In the present study, the effect of an extract of immature Prunus salicina Lindl. cv. Soldam fruit on the viability and induction of apoptosis in human hepatocellular carcinoma HepG2 cells was investigated. The results showed that in comparison with other cancer cells, the growth inhibition exerted by immature plum extracts was greatest in HepG2. Apoptosis in HepG2 cells mediated by immature plums was associated with "death receptor signaling." Immature plum extracts significantly increased the activation of caspase-8, -10, and -3 and expression of the caspase-3 target proteins alpha-fodrin (induces membrane blebbing and cell shrinkage), poly(ADP-ribose) polymerase (a nuclear enzyme that is involved in DNA repair following DNA nicks), and DNA fragmentation factor (induces apoptotic DNA fragmentation). The total yield of identified polyphenols in immature plum extract was 10 g/kg dry weight. The major components, (-)-epicatechin and (-)-gallocatechin gallate, were 34.7% and 28.6% of total polyphenols, respectively. (+)-Catechin, (-)-epicatechin gallate, and (-)-catechin gallate were also found. On the basis of these results, the immature plum (P. salicina Lindl. cv. Soldam) and its active compound, (-)-epicatechin, are expected to be a natural resource for developing novel therapeutic agents for cancer prevention and treatment.
Yu
Mi Hee
MH
Department of Food Science and Technology, Keimyung University, Daegu, Republic of Korea.
Im
Hyo Gwon
HG
Kim
Hyuk-Il
HI
Lee
In-Seon
IS
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma / Q000188:drug therapy* / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D002392:Catechin / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000378:metabolism; D005638:Fruit; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D027861:Prunus / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D013049:Spectrin
D000972:Antineoplastic Agents, Phytogenic; D002352:Carrier Proteins; D020169:Caspases; D002392:Catechin; D053418:Death Domain Receptor Signaling Adaptor Proteins; D008840:Microfilament Proteins; D010936:Plant Extracts; D011065:Poly(ADP-ribose) Polymerases; D013049:Spectrin