Molecular screening of interleukin-6 gene promoter and influence of -174G/C polymorphism on breast cancer.
Interleukin-6 (IL-6) is a cytokine involved in different physiologic and pathophysiologic processes including carcinogenesis. In 2003, a single nucleotide polymorphism (-174G/C) of the IL-6 gene promoter has been linked to breast cancer prognosis in node-positive (N+) breast cancer patients. Since, different studies have led to conflicting conclusions about its role as a prognostic and/or diagnostic marker. The primary aim of our study was to investigate the link between -174G/C polymorphism and breast cancer risk on the one hand, and -174G/C polymorphism and prognosis in different groups of patients: sporadic N+breast cancers (n=138), sporadic N- breast cancers (n=95) and familial breast cancer (n=60) on the other hand. The variables of interest were disease-free survival and overall survival. The secondary aim of the study was to screen IL-6 gene promoter using direct sequencing to identify new polymorphisms in our French Caucasian breast cancer population. No association or trend of association between -174G/C polymorphism of IL-6 gene promoter gene and breast cancer diagnosis or prognosis was shown, even in meta-analyses. Furthermore, we have identified four novel polymorphic sites in the IL-6 gene promoter region: -764G-->A, -757C-->T, -233T-->A, 15C-->A.
Chérel
Michel
M
Département de Recherche en Cancérologie, Université de Nantes, INSERM U892, 9 quai Moncousu, 44035 Nantes Cedex, France.
Campion
Loïc
L
Bézieau
Stéphane
S
Campone
Mario
M
Charrier
Josiane
J
Gaschet
Joëlle
J
Ricolleau
Gabriel
G
Gouraud
Wilfried
W
Charbonnel
Catherine
C
Jézéquel
Pascal
P
D000328:Adult; D001483:Base Sequence; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D018572:Disease-Free Survival; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
D005819:Genetic Markers; D015850:Interleukin-6