Reassessment of microarray expression data of porokeratosis by quantitative real-time polymerase chain reaction.
BACKGROUND
Porokeratosis (PK) is a heterogeneous group of keratinization disorders that exhibit similarities with psoriasis at both the clinical and molecular levels.


METHODS
The transcript levels of keratin (KRT) 6A, 16, 17, S100A7, A8, A9, p53 and three candidate genes (i.e. SART3, SSH1 and ARPC3) were reassessed in pairwise lesional and uninvolved skin from nine patients with PK by real-time quantitative polymerase chain reaction (RTQ-PCR).


RESULTS
The results of RTQ-PCR confirmed that KRT6A, 16, S100A7, A8 and A9 (p = 0.008) were mostly up-regulated in the lesional skin when compared with uninvolved skin. Different from the microarray data, there was no significant difference observed in KRT17 expression patterns between lesional and normal-appearing skin (p = 0.066). No statistical difference was observed in p53 and three candidate genes' expression patterns between lesional and uninvolved skin.


CONCLUSIONS
In the present study, 9 of the 10 gene expression measured by RTQ-PCR in PK were statistically comparable to microarray data. KRT6A was identified as specific biomarker for porokeratotic keratinocytes, as it was the most significantly up-regulated gene in the nine patient samples.
Zhang
Zheng-Hua
ZH
Department of Dermatology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China. verzhang@gmail.com
Wang
Zhi-Min
ZM
Crosby
Meredith E
ME
Kang
Ke-Fei
KF
Luan
Jing
J
Huang
Wei
W
Xiang
Lei-Hong
LH
Zheng
Zhi-Zhong
ZZ
D017668:Age of Onset; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017499:Porokeratosis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*; D046888:Tissue Array Analysis
D012333:RNA, Messenger