title: Proteome and system ontology of hemorrhagic shock: exploring early constitutive changes in postshock mesenteric lymph.
abstract: BACKGROUND
  Postshock mesenteric lymph (PSML) is the mechanistic link between splanchnic ischemia reperfusion (IR) and remote organ injury. We hypothesize that an unbiased inspection of the proteome of PSML will reveal previously unrecognized aberrations in systems biology provoked by hemorrhage-induced mesenteric IR injury in vivo.
  
  
  METHODS
  Shock was induced in male Sprague-Dawley rats by controlled hemorrhage, and the mesenteric duct was cannulated for lymph collection. Preshock and postshock lymph were collected for differential in-gel electrophoresis (DIGE)-based proteomics. Proteins that increased or decreased in relative concentration > or =1.5-fold were selected for trypsin digestion and analysis by mass spectrometry (MS).
  
  
  RESULTS
  Evidence of tissue injury was detected by an increase in cell/tissue proteins in PSML. Components of coagulation were depleted, whereas products of hemolysis were increased. Haptoglobin was decreased, which supports an early postshock hemolytic process. Interestingly, several protective protease inhibitors were decreased in PSML. The unexpected findings were an increase in alpha-enolase (a key glycolitic enzyme and cell-surface plasminogen binding receptor, +2.4-fold change) and increased major urinary protein (MUP, a sex-specific lipid-binding protein, +17.1-fold change) in PSML.
  
  
  CONCLUSIONS
  A proteomic evaluation of PSML revealed evidence of several shock-associated processes: protein release from tissue injury, depletion of coagulation factors and evidence of hemolysis, depletion of protective protease inhibitors, and an increase in abundance of lipid carriers. These results suggest that constitutive changes in the proteome of PSML may provide novel insights into the complex pathophysiology of postshock systems biology.
authors.affiliation: Department of Surgery, School of Medicine, University of Colorado Denver, Aurora, CO 80204, USA.
authors.forename: Erik D
authors.initials: ED
authors.lastname: Peltz
authors.forename: Ernest E
authors.initials: EE
authors.lastname: Moore
authors.forename: Ashley A
authors.initials: AA
authors.lastname: Zurawel
authors.forename: Janeen R
authors.initials: JR
authors.lastname: Jordan
authors.forename: Sagar S
authors.initials: SS
authors.lastname: Damle
authors.forename: Jasmina S
authors.initials: JS
authors.lastname: Redzic
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Masuno
authors.forename: John
authors.initials: J
authors.lastname: Eun
authors.forename: Kirk C
authors.initials: KC
authors.lastname: Hansen
authors.forename: Anirban
authors.initials: A
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D008196:Lymph / Q000737:chemistry* / Q000166:cytology; D008297:Male; D013058:Mass Spectrometry; D008643:Mesentery; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism; D012771:Shock, Hemorrhagic / Q000378:metabolism*