Overexpression of Mn superoxide dismutase does not increase life span in mice.
Genetic manipulations of Mn superoxide dismutase (MnSOD), SOD2 expression have demonstrated that altering the level of MnSOD activity is critical for cellular function and life span in invertebrates. In mammals, Sod2 homozygous knockout mice die shortly after birth, and alterations of MnSOD levels are correlated with changes in oxidative damage and in the generation of mitochondrial reactive oxygen species. In this study, we directly tested the effects of overexpressing MnSOD in young (4-6 months) and old (26-28 months) mice on mitochondrial function, levels of oxidative damage or stress, life span, and end-of-life pathology. Our data show that an approximately twofold overexpression of MnSOD throughout life in mice resulted in decreased lipid peroxidation, increased resistance against paraquat-induced oxidative stress, and decreased age-related decline in mitochondrial ATP production. However, this change in MnSOD expression did not alter either life span or age-related pathology.
Jang
Youngmok C
YC
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78245-3207, USA.
Pérez
Viviana I
VI
Song
Wook
W
Lustgarten
Michael S
MS
Salmon
Adam B
AB
Mele
James
J
Qi
Wenbo
W
Liu
Yuhong
Y
Liang
Hanyu
H
Chaudhuri
Asish
A
Ikeno
Yuji
Y
Epstein
Charles J
CJ
Van Remmen
Holly
H
Richardson
Arlan
A
D000375:Aging / Q000378:metabolism*; D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008931:Mitochondria, Muscle / Q000502:physiology; D018482:Muscle, Skeletal / Q000473:pathology; D009929:Organ Size; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000502:physiology*; D000099156:Superoxide Dismutase 2
D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2