title: Detection of gingival crevicular fluid MMP-8 levels with different laboratory and chair-side methods.
abstract: OBJECTIVE
  The aim of the study was to compare four methods for gingival crevicular fluid (GCF) matrix metalloproteinase (MMP)-8 detection.
  
  
  METHODS
  Matrix metalloproteinase-8 levels from 20 GCF samples from two periodontally healthy subjects, 18 samples from two patients with gingivitis and 45 samples from six patients with moderate to severe periodontitis, altogether 83 samples, were analysed using (1) a time-resolved immunofluorometric assay (IFMA), (2) an MMP-8 specific chair-side dip-stick test, (3) a dentoAnalyzer device and (4) the Amersham ELISA kit. Western immunoblot using same monoclonal anti-MMP-8 as in IFMA and dentoAnalyzer was used to identify molecular forms of MMP-8 in GCFs.
  
  
  RESULTS
  Correlation between IFMA and dentoAnalyzer results calculated with Spearman's correlation coefficient was 0.95 (P = 0.01). The chair-side dip-stick test results were well in line with these assays. Periodontitis sites with unstable characteristics were differentiated with these methods. The Amersham ELISA results were not in line with the findings by other methods.
  
  
  CONCLUSIONS
  Immunofluorometric assay and dentoAnalyzer can detect MMP-8 from GCF samples and these methods are comparable. Using Western immunoblot, it was confirmed that IFMA and dentoAnalyzer can detect activated 55 kDa MMP-8 species especially in periodontitis-affected GCF. dentoAnalyzer is among the first quantitative MMP-8 chair-side testing devices in periodontal and peri-implant diagnostics and research.
authors.affiliation: Institute of Dentistry, University of Helsinki, 00014 Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: J
authors.initials: J
authors.lastname: Leppilahti
authors.forename: S
authors.initials: S
authors.lastname: Munjal
authors.forename: L
authors.initials: L
authors.lastname: Netuschil
authors.forename: P
authors.initials: P
authors.lastname: Mäntylä
mesh_terms: D016022:Case-Control Studies; D057075:Enzyme Assays / Q000379:methods*; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology; D006801:Humans; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D010518:Periodontitis / Q000201:enzymology
chemical_list: D020784:Matrix Metalloproteinase 8