title: Direct observation of the binding process between protein and quantum dots by in situ surface plasmon resonance measurements.
abstract: A layer-by-layer surface decoration technique has been developed to anchor quantum dots (QDs) onto a gold substrate and an in situ surface plasmon resonance technique has been used to study interactions between the QDs and different proteins. Direct observation of the binding of the protein onto the QDs and the kinetics of the adsorption and dissociation of different proteins on the QDs has been achieved. This would be helpful for the identification of particle-associated proteins and may offer a fundamental prerequisite for nanobiology, nanomedicine and nanotoxicology. The combination of the novel layer-by-layer surface modification method and in situ surface plasmon resonance would be powerful in studying biological systems such as DNA and cells.
authors.affiliation: State Key Laboratory of Virology, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.forename: Fangfang
authors.initials: F
authors.lastname: Tian
authors.forename: Hongliang
authors.initials: H
authors.lastname: Guan
authors.forename: Yushu
authors.initials: Y
authors.lastname: Ge
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liu
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D004560:Electricity; D006046:Gold / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D045663:Quantum Dots; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
chemical_list: D011506:Proteins; D006046:Gold