[A novel strategy for development of universal tumor vaccine: a DC-targeted adenovirus encoding hTRT].
OBJECTIVE
To explore the feasibility of constructing a dendritic cell targeted adenovirus as a possible universal tumor vaccine.


METHODS
The cDNA of human telomerase reverse transcriptase (hTRT) from pBABE-PURO-hTERT was subcloned into pAdTrack-CMV. Recombinant adenovirus for hTRT was constructed in AdEasy system. The confirmed recombinant adenovirus plasmid was transfected into 293 cells to generate the recombinant adenovirus. Later the recombinant adenovirus was multiplied, purified and modified with mannan as a vaccine to immunize mice.


RESULTS
An obvious cytopathic effect was observed with the rescue of recombinant adenovirus. The expression of hTRT was confirmed by PCR and Western blot analysis. Mannan modified adenovirus stained positively by periodic acid Schiff stain, and expression of the adenovirus was found mainly restricted in dendritic cells (DC) in spleen of mice.


CONCLUSIONS
A DC-targeted adenovirus was constructed. This might be helpful (or the development of universal tumor vaccine in future.
Ding
Zhen-yu
ZY
Laboratory of Tumor Biotherapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Wang
Chun
C
Su
Jing-mei
JM
Wei
Yu-quan
YQ
Wang
Chun-ting
CT
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D008351:Mannans / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D019098:Telomerase / Q000235:genetics*; D014162:Transfection
D019496:Cancer Vaccines; D018076:DNA, Complementary; D008351:Mannans; C509186:TERT protein, human; D019098:Telomerase