Serum levels of p53 and cytochrome c in subjects with type 2 diabetes and impaired glucose tolerance.
OBJECTIVE
To examine apoptotic markers in serum of subjects with diabetes and impaired glucose tolerance (IGT). Serum levels of p53 and cytochrome c, regulator molecules for apoptosis, were measured in subjects with type 2 diabetes, subjects with IGT and healthy controls.


METHODS
Forty one subjects with type 2 diabetes, 27 with IGT and 27 healthy volunteers were included in the study. Serum level of cytochrome c and p53 were measured with competitive ELISA.


RESULTS
Serum levels of p53 were lower in the group of subjects with type 2 diabetes (085+/-0.39 U/ml) than in controls (1.09+/-0.49 U/ml) (P < 0.05) and in the subjects with IGT (0.98+/-0.37 U/ml) (P < 0.05). There was no significant difference between the group with IGT and controls. Also, there was no difference for serum level of cytochrome c among the groups. In the group of subjects with type 2 diabetes, serum level of cytochrome c was mildly correlated with HbA1c (r:0.39, P < 0.05).


CONCLUSIONS
The present study shows that the serum level of p53 is lower in the patients with type 2 diabetes than in controls or in subjects with IGT. No difference was seen among the the groups for the serum level of cytochrome c.
Dincer
Yildiz
Y
Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry. stare63@yahoo.com
Himmetoglu
Solen
S
Yalin
Serap
S
Damci
Taner
T
Ilkova
Hasan
H
Akcay
Tulay
T
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D045304:Cytochromes c / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018149:Glucose Intolerance / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016159:Tumor Suppressor Protein p53 / Q000097:blood*
D016159:Tumor Suppressor Protein p53; D045304:Cytochromes c