title: l-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide.
abstract: We examined whether nitric oxide (NO) in vivo could induce interscapular brown adipose tissue (IBAT) glutathione synthesis. Data show that NO induces in vivo IBAT glutathione synthesis through activation of glutamate-cysteine ligase (GCL) mRNA and protein expression. This NO effect appeared to be mediated by nuclear factor-kappaB (NF-kappaB) activation. We have also observed a complex series of in vivo cellular responses during chronic inhibition of NO synthesis, suggesting that regulatory pathways unrelated to GCL alteration underlie glutathione level increase induced by N(omega)-nitro-l-arginine methyl ester (l-NAME). In general, glutathione synthesis in IBAT seemed to be finely tuned by NO to provide glutathione for either mediating the effects of NO, or for preventing potential nitrosative stress.
authors.affiliation: University of Belgrade, Institute for Biological Research Sinisa Stanković, Department of Physiology, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Petrović
authors.forename: Biljana
authors.initials: B
authors.lastname: Buzadzić
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Korać
authors.forename: Ana
authors.initials: A
authors.lastname: Vasilijević
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Janković
authors.forename: Bato
authors.initials: B
authors.lastname: Korać
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001120:Arginine; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D015870:Gene Expression; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
chemical_list: D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D009569:Nitric Oxide; D001120:Arginine; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D019331:NG-Nitroarginine Methyl Ester