Verrucosispora lutea sp. nov., isolated from a mangrove sediment sample.
A novel Gram-positive, aerobic, spore-forming actinobacterium, designated strain YIM 013T, was isolated from a mangrove sediment sample and subjected to a polyphasic taxonomic study. The peptidoglycan type was A1gamma and the major whole-cell sugars contained glucose and xylose. The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and some unknown phospholipids. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C15:0 and iso-C16:0. The genomic DNA G+C content was 69.3 mol%. blast search results based on an almost-complete 16S rRNA gene sequence showed that strain YIM 013T had the highest similarity with Verrucosispora gifhornensis DSM 44337T (98%) and phylogenetic analysis revealed that strain YIM 013T should be assigned to the genus Verrucosispora. The mean level of DNA-DNA relatedness between strain YIM 013T and V. gifhornensis DSM 44337T was 38.2%. Based on its phenotypic and genotypic characteristics, strain YIM 013T (=CCTCC AA207012T=KCTC 19195T) is proposed to be the type strain of a novel species, Verrucosispora lutea sp. nov.
Liao
Zhen-Lin
ZL
Food Science College, South China Agricultural University, Guangzhou 510642, PR China.
Tang
Shu-Kun
SK
Guo
Lin
L
Zhang
Yu-Qin
YQ
Tian
Xin-Peng
XP
Jiang
Cheng-Lin
CL
Xu
Li-Hua
LH
Li
Wen-Jun
WJ
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008848:Micromonosporaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010944:Plants / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology; D024482:Vitamin K 2 / Q000032:analysis; D014994:Xylose / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D014994:Xylose; D005947:Glucose