title: Diagnostic importance of overexpression of Bmi-1 mRNA in early breast cancers.
abstract: Target molecules for a highly sensitive and specific diagnosis of breast cancer in its early clinical stages have not yet been identified. Here, we show the first evidence for diagnostic performance of the molecule B cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) in breast cancer patients. Only 5 out of 46 non-cancerous samples were positive for Bmi-1 mRNA expression resulting in a sensitivity and specificity of 72.0 and 91.3%, respectively. The mRNA expression was estimated using the cut-off value obtained from the receiver operating characteristic curve analysis. Further, Bmi-1 mRNA expression was found to be elevated in 97.8% (45/46) of cancerous tissues in comparison to the expression in paired cancerous tissues and non-cancerous tissues obtained from identical patients. Bmi-1 mRNA was found to be highly expressed even in the early clinical stages of breast cancer. Our results suggest that Bmi-1 mRNA might be a new tool to support the diagnosis of breast cancers, irrespective of the clinical stage.
authors.affiliation: Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Chuo-Ku, Sapporo, Japan.
authors.forename: Masachika
authors.initials: M
authors.lastname: Saeki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Tsuji
authors.forename: Kageaki
authors.initials: K
authors.lastname: Kuribayashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D063150:Polycomb Repressive Complex 1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012372:ROC Curve; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: C069077:BMI1 protein, human; D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1