title: Selection of the optimal solvent grade for the mobile phase in HPLC with electrochemical detection based on FUMI theory.
abstract: The optimum conditions of the mobile phase for HPLC with electrochemical detection (HPLC-ECD) were selected from among solvents of different grades with the standard deviation (SD) of area measurements based on FUMI (function of mutual information) theory as a criterion. In HPLC-ECD for determining baicalin and baicalein using a phosphoric acid-methanol-water mixture (0.5:60:40, v/v/v) as a mobile phase, the effects of three grades of phosphoric acid, of five grades of methanol, and of seven grades of water on the measurement precision were examined. The mixture, if characterized by the minimum SD of measurements, was selected as the optimal mobile phase. This selection method saves considerable amounts of chemicals and experimental time, and would be a useful exploration technique for the routine check and troubleshooting of HPLC-ECD.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan. kotani@toyaku.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Kotani
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Rieko
authors.initials: R
authors.lastname: Matsuda
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Kusu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D020835:Medicine, Kampo; D012997:Solvents / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D012997:Solvents; C461320:shosaiko-to; C038044:baicalin; C006680:baicalein