[Effects of genistein on interleukin-1beta and tumor necrosis factor-alpha secreted by fibroblast-like synoviocytes isolated from type II collagen-induced arthritis rats].
OBJECTIVE
To investigate the effects of genistein (Gen) on interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) secreted by fibroblast-like synoviocytes (FLSs) of rats with type II collagen-induced arthritis (CIA).


METHODS
Type II collagen was injected to induce arthritis in rats, and arthritis index was applied to evaluate whether the arthritis was induced successfully. The primary FLSs were separated from synovial membranes by using type II collagenase digestion. Then the expression of vascular cell adhesion molecule-1 (VCAM-1) was estimated by flow cytometry (FCM). After addition of different concentrations of Gen into the FLSs, IL-1beta and TNF-alpha contents in the supernatants were measured by enzyme-linked immunosorbent assay.


RESULTS
Three days after collagen injection, the rats started to develop arthrocele and the arthritis index increased gradually. The arthritis index of CIA group was significantly higher than that of the control group. The expression of VCAM-1 was up to 85.5% in the 4th generation FLSs, indicating that most part of the cultured cells were type-B synoviocytes. After administration of different concentrations of Gen (100, 200, 400 micromol/L), the contents of IL-1beta and TNF-alpha in supernatants of FLSs were decreased dose-dependently.


CONCLUSIONS
Genistein can suppress the secretion of TNF-alpha and IL-1beta in FLSs dose-dependently, which may be one of the mechanisms for genistein in inhibiting the arthromeningitis of CIA rats.
Gao
Bo
B
Department of Hematopathy and Rheumatism, Clinical Medicine College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
Zhang
Yu
Y
Ma
Li
L
Zhang
Xue-zeng
XZ
D000818:Animals; D001169:Arthritis, Experimental / Q000209:etiology / Q000378:metabolism*; D024043:Collagen Type II; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism*; D007529:Isoflavones / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D024043:Collagen Type II; D053583:Interleukin-1beta; D007529:Isoflavones; D014409:Tumor Necrosis Factor-alpha; D019833:Genistein