Adrenomedullin, Bcl-2 and microvessel density in normal, hyperplastic and neoplastic endometrium.
Adrenomedullin (ADM) is a multifunctional 52-amino acid peptide involved in numerous physiological and pathological processes, including angiogenesis, growth regulation, differentiation, and vasodilation. ADM is thought to act through the G protein-coupled receptor calcitonin receptor-like receptor, with specificity being conferred by receptor-associated modifying protein 2. The aim of the present study was to clarify the roles of ADM status, and tumor vessels in endometrium. Specimens were examined for ADM, microvessel density (MVD), area of venules (AV) and Bcl-2 oncoprotein using an immunoperoxidase method. The difference of ADM between normal proliferative phase and hyperplasia without atypia was significant (P < 0.05). The level of Bcl-2 was significantly different between hyperplasia without atypia and hyperplasia with atypia (P < 0.05). ADM, MVD and AV in the endometrium increased in a stepwise manner from normal, simple or complex hyperplasia with or without atypia to grade 1 adenocarcinoma. In contrast, expression of Bcl-2 oncoprotein was decreased. These parameters identify the role of ADM expression and Bcl-2 protein in relation to cell growth and vasodilating in the neoplastic changes.
Nunobiki
Osamu
O
Department of Medical Technology, Kobe Tokiwa University, Kobe, Hyogo, Japan. nunobiki@kobe-tokiwa.ac.jp
Nakamura
Misa
M
Taniguchi
Emiko
E
Utsunomiya
Hirotoshi
H
Mori
Ichiro
I
Tsubota
Yukari
Y
Mabuchi
Yoshiya
Y
Kakudo
Kennichi
K
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D053607:Adrenomedullin / Q000096:biosynthesis*; D000328:Adult; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000098:blood supply / Q000473:pathology; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D055806:Microvessels; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*
D019253:Proto-Oncogene Proteins c-bcl-2; D053607:Adrenomedullin