title: Intestinal lengthening in rats after massive small intestinal resection.
abstract: BACKGROUND
  Prior studies have demonstrated lengthening of normal small intestinal segments using mechanical expanders. The present study assesses the feasibility of intestinal lengthening in rats that have undergone extensive small intestinal resection.
  
  
  METHODS
  Female rats underwent small intestinal resection. After 6 weeks, the animals underwent the placement of a mechanical expander device with or without gradual mechanical lengthening. After 3 weeks, the intestinal segments were retrieved for analyses.
  
  
  RESULTS
  Isolated intestinal segments without mechanical lengthening did not change in length, whereas isolated intestinal segments that were mechanically lengthened more than doubled their initial length. The total alkaline phosphatase activity was 2.4 mumol/min in the isolated intestinal segments and 4.9 mumol/min in the mechanically lengthened segments. The total lactase activity was 0.005 mumol/min in the isolated intestinal segments and 0.007 mumol/min in the mechanically lengthened segments. Smooth muscle thickness was 370 mum in the isolated intestinal segments and 530 mum in the mechanically lengthened segments.
  
  
  CONCLUSIONS
  Mechanical small bowel lengthening was achieved in intestinal segments after extensive small intestinal resection. There was an increase in the total alkaline phosphatase activity and preservation of the total lactase activity. Mechanical lengthening may be a useful technique to increase intestinal length in patients with short bowel syndrome.
authors.affiliation: Department of Surgery, Division of Pediatric Surgery, Department of Bioengineering, Interdepartmental Program in Biomedical Engineering, University of California, Los Angeles, CA 90095, USA.
authors.forename: Shant
authors.initials: S
authors.lastname: Shekherdimian
authors.forename: Andrew
authors.initials: A
authors.lastname: Scott
authors.forename: Andrea
authors.initials: A
authors.lastname: Chan
authors.forename: James C Y
authors.initials: JC
authors.lastname: Dunn
mesh_terms: D000222:Adaptation, Physiological; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D013505:Digestive System Surgical Procedures; D005240:Feasibility Studies; D005260:Female; D007421:Intestine, Small / Q000201:enzymology / Q000473:pathology / Q000502:physiology / Q000601:surgery*; D043322:Lactase / Q000378:metabolism; D009130:Muscle, Smooth / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012778:Short Bowel Syndrome / Q000601:surgery; D015626:Tissue Expansion; D015625:Tissue Expansion Devices
chemical_list: D000469:Alkaline Phosphatase; D043322:Lactase