The plasma membrane proteome of germinating barley embryos.
Cereal seed germination involves a complex coordination between different seed tissues. Plasma membranes must play crucial roles in coordination and execution of germination; however, very little is known about seed plasma membrane proteomes due to limited tissue amounts combined with amphiphilicity and low abundance of membrane proteins. A fraction enriched in plasma membranes was prepared from embryos dissected from 18 h germinated barley seeds using aqueous two-phase partitioning. Reversed-phase chromatography on C(4) resin performed in micro-spin columns with stepwise elution by 2-propanol was used to reduce soluble protein contamination and enrich for hydrophobic proteins. Sixty-one proteins in 14 SDS-PAGE bands were identified by LC-MS/MS and database searches. The identifications provide new insight into the plasma membrane functions in seed germination.
Hynek
Radovan
R
Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Svensson
Birte
B
Jensen
Ole Nørregaard
ON
Barkholt
Vibeke
V
Finnie
Christine
C
D002462:Cell Membrane / Q000378:metabolism*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D018525:Germination / Q000502:physiology*; D001467:Hordeum / Q000378:metabolism* / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism* / Q000502:physiology*; D053719:Tandem Mass Spectrometry
D008565:Membrane Proteins; D010940:Plant Proteins