[Changes of expression levels of innate immunity signaling receptors during Candida albicans infections in vitro and in vivo].
OBJECTIVE
To study levels of expression of Toll-like receptors genes in response to Candida albicans antigens in vitro (using Vero cell line as well as mononuclear cells) and in vivo (using cervical canal cells of pregnant women).


METHODS
Test-systems for measurement of expression levels of such genes as TLR 1, TLR2, TLR6 as well as system for quantitative measurement of tumor necrosis factor a level, all of which were developed earlier, were used.


RESULTS
It was shown that antigens of C. albicans stimulated early increase of expression of innate immunity genes both in studied in vitro models and in cells of cervical canal of pregnant women with candidosis.


CONCLUSIONS
Results of performed study allow to suggest that activation of innate immunity factors resulted from Candida albicans infection.
Gankovskaia
O A
OA
Zverev
V V
VV
Lavrov
V F
VF
Blinkova
L P
LP
Gankovskaia
L V
LV
Kuznetsov
P A
PA
D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000235:genetics / Q000276:immunology*; D002584:Cervix Uteri / Q000276:immunology* / Q000378:metabolism; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000235:genetics / Q000276:immunology*; D051194:Toll-Like Receptor 1 / Q000235:genetics*; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051216:Toll-Like Receptor 6 / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D015854:Up-Regulation; D014709:Vero Cells
D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051216:Toll-Like Receptor 6; D014409:Tumor Necrosis Factor-alpha