title: Small interfering RNA targeting bovine papillomavirus type 1 E2 induces apoptosis in equine sarcoid transformed fibroblasts.
abstract: Equine sarcoids are skin tumours of horses caused by infection with BPV-1 or 2. Maintenance and replication of the viral genome depend upon the viral proteins E1 and E2. We examined the effects of an E2 specific siRNA on E2 and E1 viral gene expression, viral load and cell growth in BPV-1 transformed sarcoid-derived cells. Transfection with E2-siRNA caused a reduction in E2 and E1 mRNA expression as well as viral load, growth inhibition and decreased anchorage-independent growth. siRNA treated cells showed significantly higher apoptosis rates than control cells. Thus sequence specific targeting of E2 provides a powerful strategy to eliminate BPV-1 genomes and induce cell death in BPV-1 transformed cells.
authors.affiliation: Institute of Comparative Medicine, University of Glasgow Faculty of Veterinary Medicine, Garscube Campus, Scotland, UK.
authors.forename: Philipe A M
authors.initials: PA
authors.lastname: Gobeil
authors.forename: ZhengQiang
authors.initials: Z
authors.lastname: Yuan
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Gault
authors.forename: Iain M
authors.initials: IM
authors.lastname: Morgan
authors.forename: M Saveria
authors.initials: MS
authors.lastname: Campo
authors.forename: Lubna
authors.initials: L
authors.lastname: Nasir
mesh_terms: D000818:Animals; D017209:Apoptosis; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology* / Q000821:virology; D015870:Gene Expression; D015316:Genetic Therapy; D016679:Genome, Viral; D006736:Horses; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000628:therapy / Q000821:virology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C069814:E1 protein, Bovine papillomavirus; C051550:E2 protein, Bovine papillomavirus; D034741:RNA, Small Interfering; D012367:RNA, Viral; D014764:Viral Proteins