title: Transforming growth factor-alpha in a defined medium during in vitro maturation of porcine oocytes improves their developmental competence and intracellular ultrastructure.
abstract: We examined the effects of transforming growth factor-alpha (TGF-alpha) to develop a defined medium for in vitro maturation (IVM) of porcine (Sus scrofa domesticus) oocytes. Cumulus-oocyte complexes (COCs) were matured in porcine oocyte medium containing 3mg/mL polyvinyl alcohol (POM) and TGF-alpha (0, 1, 10, or 100 ng/mL) in the presence or absence of the gonadotropins equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). In the absence of gonadotropins, adding 10 ng/mL TGF-alpha increased (P<0.05) the percentage of oocytes that reached metaphase II (24.2%) compared with that of the control (no TGF-alpha addition; 5.6%). In the presence of gonadotropins, although maturation rate did not differ among TGF-alpha treatments (75.4% to 84.8%), the rate of blastocyst formation (28.1%) was higher (P<0.05) in the TGF-alpha group (28.1%) than that in the control group (15.9%) after in vitro fertilization and embryo culture. An electron microscope study revealed that TGF-alpha-treated oocytes contained more homogenous lipid droplets than did control oocytes. Moreover, mitochondria surrounded by the endoplasmic reticulum were observed only in the TGF-alpha-treated oocytes. In blastocysts derived from the latter oocytes, mitochondria with numerous cristae were frequently observed compared with that in blastocysts from control oocytes. When the Day-5 blastocysts obtained from oocytes matured with TGF-alpha were surgically transferred into four recipients, a total of 29 piglets were farrowed. We concluded that the addition of TGF-alpha to the defined IVM medium of porcine oocytes improved the subsequent blastocyst formation and that the blastocysts produced by the defined in vitro production system have developmental competence to full term after embryo transfer.
authors.affiliation: Research Institute for the Functional Peptides, Shimojo, Yamagata, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mito
authors.forename: K
authors.initials: K
authors.lastname: Yoshioka
authors.forename: M
authors.initials: M
authors.lastname: Nagano
authors.forename: C
authors.initials: C
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: H
authors.initials: H
authors.lastname: Hoshi
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000648:ultrastructure; D018929:Cell Culture Techniques; D003470:Culture Media; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005307:Fertilization in Vitro; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D034421:Sus scrofa / Q000196:embryology*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D016211:Transforming Growth Factor alpha