title: Cryptococcus ibericus sp. nov., Cryptococcus aciditolerans sp. nov. and Cryptococcus metallitolerans sp. nov., a new ecoclade of anamorphic basidiomycetous yeast species from an extreme environment associated with acid rock drainage in São Domingos pyrite mine, Portugal.
abstract: In this report, we describe three novel asexual basidiomycetous yeast species, Cryptococcus aciditolerans sp. nov. (type strain CBS 10872T=SDY 081T), Cryptococcus ibericus sp. nov. (type strain CBS 10871T=SDY 022T) and Cryptococcus metallitolerans sp. nov. (type strain CBS 10873T=SDY 190T), which were isolated from acid rock drainage collected at the São Domingos mine in southern Portugal. Phylogenetic analysis of molecular sequence data indicated that the novel species belong to the order Filobasidiales of the class Tremellomycetes and form a well-separated clade, next to Cryptococcus gastricus and Cryptococcus gilvescens. Since the novel species also share a peculiar ecology, being able to thrive under extreme environmental conditions characterized by very low pH and high concentrations of heavy metals, we designate this combination of phylogenetic and ecological characteristics as an ecoclade.
authors.affiliation: Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Fundação da Faculdade de Ciências de Lisboa, Edifício ICAT-Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal. mjgadanho@fc.ul.pt
authors.forename: Mário
authors.initials: M
authors.lastname: Gadanho
authors.forename: José Paulo
authors.initials: JP
authors.lastname: Sampaio
mesh_terms: D016000:Cluster Analysis; D003454:Cryptococcus / Q000145:classification* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D025141:Drug Resistance, Fungal; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D011174:Portugal; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D019216:Metals, Heavy; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S