[Study on the expression of BDNF in human gliomas].
OBJECTIVE
To investigate the expression of brain-derived neurotrophic factors (BDNF) in gliomas of human, and explore the relation between BDNF and pathologic grading and tumorigenesis of gliomas.


METHODS
Immunohistochemistry (SP) and Western blot were applied to evaluate the expression of BDNF in 5 cases of normal controls and 20 cases of gliomas from different pathologic grading.


RESULTS
The expression of BDNF was observed mainly occurred in the cytoplasm of both normal brain tissues and gliomas. Western blot analysis showed that the expression level of BDNF in gliomas was significantly higher than that of normal controls (P<0.05), and a gradually increased expression of BDNF in vary pathologic grading (grade I, II, III, IV) gliomas was also observed.


CONCLUSIONS
The expression of BDNF in gliomas of human was greatly upregulated, indicating BDNF may play an important role in the tumorigenesis of gliomas, and relate with pathologic grading.
Yan
Qi
Q
Department of Minimally Invasive Neurosurgery, No 1 Affiliated Hospital, Kunming Medical College, Kunming 650031, China.
Yu
Hua-lin
HL
Li
Jing-tao
JT
D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007120:Immunochemistry; D008297:Male; D008875:Middle Aged
D019208:Brain-Derived Neurotrophic Factor