title: Single bead labeling method for combining confocal fluorescence on-bead screening and solution validation of tagged one-bead one-compound libraries.
abstract: Screening of one-bead one-compound libraries by incubating beads with fluorescently labeled target protein requires isolation and structure elucidation of a large number of primary hit beads. However, the potency of the identified ligands is only revealed after time consuming and expensive larger scale resynthesis and testing in solution. Often, many of the resynthesized compounds turn out to be weak target binders in solution due to large differences between surface and solution binding affinities. For an industry style high-throughput screening (HTS) process a high false positive rate is detrimental. We have therefore combined single bead and single molecule/single cell techniques into an integrated HTS process in which the picomole amount of substance contained on one isolated hit bead is sufficient for quality control, structure determination, and precise affinity determination to the target protein in solution.
authors.affiliation: University of Edinburgh, School of Biological Sciences, The King's Buildings, CH Waddington Building 3.07, Mayfield Road, Edinburgh EH9 3JR, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Hintersteiner
authors.forename: Thierry
authors.initials: T
authors.lastname: Kimmerlin
authors.forename: Frank
authors.initials: F
authors.lastname: Kalthoff
authors.forename: Markus
authors.initials: M
authors.lastname: Stoeckli
authors.forename: Geraldine
authors.initials: G
authors.lastname: Garavel
authors.forename: Jan-Marcus
authors.initials: JM
authors.lastname: Seifert
authors.forename: Nicole-Claudia
authors.initials: NC
authors.lastname: Meisner
authors.forename: Volker
authors.initials: V
authors.lastname: Uhl
authors.forename: Christof
authors.initials: C
authors.lastname: Buehler
authors.forename: Thomas
authors.initials: T
authors.lastname: Weidemann
authors.forename: Manfred
authors.initials: M
authors.lastname: Auer
mesh_terms: D055808:Drug Discovery / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005453:Fluorescence; D008024:Ligands; D008722:Methods; D008863:Microspheres; D015336:Molecular Probe Techniques; D054852:Small Molecule Libraries
chemical_list: D008024:Ligands; D054852:Small Molecule Libraries