[Analysis of PARP10 tissue expression profile, interactive protein and UV stress reaction].
One pair of primers were designed and synthesized based on the cDNA sequence encoding Homo sapiens poly (ADP-ribose) polymerase family, member 10 (PARP10) reported on the GenBank. The cDNA sequence encoding PARP10 was cloned from 293FT cell by RT-PCR. Then the RT-PCR product was cloned into pCMV-Myc and pEGFP-C1 plasmids. The interaction between PARP10 and beta-actin was identified through immuno-precipitation and laser confocal microscopy. Extensive expression of PARP10 in mouse tissues was confirmed by RT-PCR. Besides, Western blotting analysis indicated that cell injury caused by UV treatment could promote the expression of PARP10. The results in this paper would benefit further study of PARP10.
Yu
Mengbin
M
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Zhao
Lixia
L
Yang
Yutao
Y
Yang
Zhixin
Z
Zhang
Yingying
Y
Zhu
Hengqi
H
Zhou
Xiaowei
X
Huang
Peitang
P
D000199:Actins / Q000378:metabolism*; D000818:Animals; D006801:Humans; D051379:Mice; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism* / Q000528:radiation effects*; D054730:Protein Interaction Domains and Motifs; D011518:Proto-Oncogene Proteins / Q000378:metabolism* / Q000528:radiation effects*; D013312:Stress, Physiological / Q000528:radiation effects; D014018:Tissue Distribution; D014466:Ultraviolet Rays
D000199:Actins; D011518:Proto-Oncogene Proteins; C499627:PARP10 protein, human; D011065:Poly(ADP-ribose) Polymerases