Phostensin caps to the pointed end of actin filaments and modulates actin dynamics.
Phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949, consists of 165 amino acids and is located between HLA-C and HLA-E gene clusters on human chromosome 6. In this current study, we characterized the biochemical functions of phostensin. Actin dynamics assays using gelsolin-actin seeds showed that phostensin decreases the elongation and depolymerization rates of actin filament pointed ends. The feature of phostensin that binds to the pointed ends of actin filaments was observed through fluorescent single filament binding assay. Taken together, our results suggested that phostensin is an actin filament pointed end-capping protein that is capable of modulating actin dynamics.
Lai
Ning-Sheng
NS
Department of Medicine, DaLin Tzu Chi Buddhist Hospital, Chia-Yi, Taiwan.
Wang
Tzu-Fan
TF
Wang
Shiu-Lan
SL
Chen
Chun-Yu
CY
Yen
Jia-yi
JY
Huang
Hsien-lu
HL
Li
Chin
C
Huang
Kuang-Yung
KY
Liu
Su-Qin
SQ
Lin
Ta-Hsien
TH
Huang
Hsien-bin
HB
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D006801:Humans; D054645:Protein Phosphatase 1 / Q000235:genetics / Q000378:metabolism*
D000199:Actins; C520010:PPP1R18 protein, human; D054645:Protein Phosphatase 1