title: The incorporation of chlorhexidine in a two-step self-etching adhesive preserves dentin bond in vitro.
abstract: OBJECTIVE
  To investigate whether the incorporation of chlorhexidine in a two-step self-etching adhesive can preserve dentin bond strengths.
  
  
  METHODS
  Different amounts of 20% chlorhexidine digluconate were added directly to the primer of Clearfil SE Bond to prepare mixtures of four different concentrations of chlorhexidine: 0.05%, 0.1%, 0.5% and 1.0%. Sixteen extracted third molars were randomly divided into 4 groups. Each group corresponded to one of the four chlorhexidine concentrations. Each of the 16 teeth was sectioned into two halves. One half was bonded with Clearfil SE Bond without chlorhexidine, and the other half was bonded with Clearfil SE Bond containing different concentrations of chlorhexidine. Specimens were stored in 0.9% NaCl containing 0.02% sodium azide at 37 degrees C. Microtensile bond strengths were tested 24h after specimen preparation or 12 months later. The modes of fractures were examined under a stereomicroscope.
  
  
  RESULTS
  Twelve-month storage resulted in significant bond strength reduction of all control groups (p<0.05). When incorporated in SE Bond primer, chlorhexidine preserved dentin bond in the 0.1%, 0.5% and 1.0% chlorhexidine group (p<0.05); in the 0.05% group, there is no statistical difference of bond strength between control group and experimental group tested at the 12-month period (p>0.05).
  
  
  CONCLUSIONS
  When incorporated in the primer of Clearfil SE Bond, chlorhexidine can preserve dentin bond as long as the concentration of chlorhexidine in the primer is higher than or equal to 0.1%.
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhou
authors.forename: Jianguo
authors.initials: J
authors.lastname: Tan
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Deli
authors.initials: D
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Tan
mesh_terms: D002710:Chlorhexidine / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D001840:Dental Bonding; D045662:Dental Etching / Q000379:methods; D003799:Dental Stress Analysis; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000378:metabolism; D008964:Molar, Third; D011480:Protease Inhibitors / Q000737:chemistry*; D019279:Resin Cements / Q000737:chemistry*; D013718:Tensile Strength
chemical_list: C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D019279:Resin Cements; D003094:Collagen; D020782:Matrix Metalloproteinases; D002710:Chlorhexidine