title: [Up-regulation of Fas is related to apoptosis of HeLa cells induced by co-immobilized TNF-alpha/IFN-gamma].
abstract: This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
authors.affiliation: College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Sun
authors.forename: Zeqin
authors.initials: Z
authors.lastname: Fang
authors.forename: Jianting
authors.initials: J
authors.lastname: Zheng
authors.forename: Zherui
authors.initials: Z
authors.lastname: Wu
authors.forename: Jinwei
authors.initials: J
authors.lastname: Qiu
authors.forename: Weifang
authors.initials: W
authors.lastname: Li
authors.forename: Dongqin
authors.initials: D
authors.lastname: Zhao
authors.forename: Shujun
authors.initials: S
authors.lastname: Liang
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Guan
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D004357:Drug Synergism; D006367:HeLa Cells; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D007371:Interferon-gamma / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000494:pharmacology*; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism*
chemical_list: C491901:FAS protein, human; D055767:Immobilized Proteins; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D007371:Interferon-gamma