Protein-functionalized hairy diamond nanoparticles.
Diazonium salt chemistry and atom transfer radical polymerization (ATRP) were combined in view of preparing new bioactive hairy diamond nanoparticles containing, or potentially containing, nitrogen-vacancy (NV) fluorescent centers (fluorescent nanodiamonds, or fNDs). fNDs were modified by ATRP initiators using the electroless reduction of the diazonium salt BF(4)(-),(+)N(2)-C(6)H(4)-CH(CH(3))-Br. The strongly bound aryl groups -C(6)H(4)-CH(CH(3))-Br efficiently initiated the ATRP of tert-butyl methacrylate (tBMA) at the surface of the nanodiamonds, which resulted in obtaining ND-PtBMA hybrids. The grafted chain thickness, estimated from X-ray photoelectron spectroscopy (XPS), was found to increase linearly with respect to time before reaching a plateau value of ca. 2 nm. These nanoobjects were further hydrolyzed into ND-PMAA (where PMAA is the poly(methacrylic acid) graft) and further decorated by bovine serum albumin through the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling procedure.
Dahoumane
Si Amar
SA
ITODYS, Universite Paris Diderot and CNRS (UMR 7086), 15 rue Jean de Baif, 75013 Paris, France.
Nguyen
Minh Ngoc
MN
Thorel
Alain
A
Boudou
Jean-Paul
JP
Chehimi
Mohamed M
MM
Mangeney
Claire
C
D000818:Animals; D002417:Cattle; D018130:Diamond / Q000737:chemistry; D003979:Diazonium Compounds / Q000737:chemistry; D004577:Electron Probe Microanalysis / Q000379:methods; D005453:Fluorescence; D006868:Hydrolysis; D008689:Methacrylates / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009584:Nitrogen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry
D003979:Diazonium Compounds; D008689:Methacrylates; D011108:Polymers; D011506:Proteins; D012492:Salts; D012709:Serum Albumin; D018130:Diamond; D009584:Nitrogen; C016284:butyl methacrylate