Typing of clinical and environmental strains of Aeromonas spp. using two PCR based methods and whole cell protein analysis.
Two PCR based typing methods i.e. random amplified polymorphic DNA analysis (RAPD) and enterobacterial repetitive intergenic consensus sequence (ERIC)-PCR were evaluated for typing of 42 Aeromonas isolates from clinical and environmental sources and whole cell protein (WCP) profiles were analyzed. Both RAPD and ERIC-PCR showed a high level of genetic diversity. Numerical index of the discriminatory (D) values were 0.94 and 0.96 (>0.90) for RAPD and ERIC-PCR, respectively. No correlation in banding pattern and evidence of genetic similarity was found between Aeromonas isolates from environmental and clinical sources. Therefore these techniques are highly reproducible and sensitive methods for typing the Aeromonas isolate from different sources. WCP profile showed two major variable regions i.e. 20 kDa to 45 kDa region and 70 kDa to 85 kDa region. Though WCP profiling had less discriminatory power, use of this method in combination with other established typing methods such as RAPD and ERIC-PCR may be helpful for reliable typing of Aeromonas isolates or to identify new proteins with pathogenic potential.
Maiti
Biswajit
B
Department of Fishery Microbiology, UNESCO-MIRCEN for Marine Biotechnology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore, 575002, India.
Raghunath
Pendru
P
Karunasagar
Iddya
I
Karunasagar
Indrani
I
D000333:Aeromonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D004783:Environmental Microbiology; D014644:Genetic Variation; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D010449:Peptide Mapping / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020543:Proteome / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D020543:Proteome