[Effect of basic fibroblast growth factor on the gene expression of epidermal growth factor receptor by periodontal ligament cells in culture].
OBJECTIVE
To study the effect of basic fibroblast growth factor (bFGF) on the gene expression of epidermal growth factor receptor (EGFR) by periodontal ligament cells (PDLC) in culture and discuss the effect of bFGF in cell differentiation and periodontal regeneration.


METHODS
Human PDLC were cultured in vitro and cell clone was obtained by the method of limiting dilution. PDLC were stimulated by bFGF, and then gene expression of EGFR was assessed by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
The mRNA expression of EGFR was promoted by bFGF and the effect was dose-dependent.


CONCLUSIONS
The promotion effect of the mRNA expression of EGFR in PDLC may be one of important factors which participate in the healing process of periodontitis and provide partly theoretical basis of cell differentiation and periodontal regeneration.
Chen
Ying
Y
Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China.
Lin
Chong-Tao
CT
Gao
Ying
Y
D002454:Cell Differentiation; D002478:Cells, Cultured; D066246:ErbB Receptors; D016222:Fibroblast Growth Factor 2; D006801:Humans; D010513:Periodontal Ligament; D012333:RNA, Messenger; D012038:Regeneration
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D066246:ErbB Receptors