Pseudorhodoferax soli gen. nov., sp. nov. and Pseudorhodoferax caeni sp. nov., two members of the class Betaproteobacteria belonging to the family Comamonadaceae.
A novel betaproteobacterium, strain TBEA3(T), was isolated from soil using enrichment cultures with the organic thioether 3,3'-thiodipropionic acid as sole carbon and energy source. Analysis of the 16S rRNA gene sequence revealed 99.1 % sequence similarities to a poorly characterized member of the family Comamonadaceae, strain SB1(T), which had been previously isolated from activated sludge. Both strains showed highest gene sequence similarities (up to 96.9 %) to members of the genera Rhodoferax and Curvibacter. The DNA G+C contents of strains TBEA3(T) and SB1(T) were 69.1 and 70.1 mol%, respectively, and the DNA-DNA hybridization value between these two strains was 45.3 %. The predominant cellular fatty acids in both strains were C(16 : 0), C(18 : 1)omega7c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH). The major 3-hydroxy fatty acid was C(10 : 0) 3-OH. Based on the genetic and chemotaxonomic data, strains TBEA3(T) and SB1(T) represent two novel species of a new genus within the family Comamonadaceae, for which the name Pseudorhodoferax gen. nov. is proposed. Strain TBEA3(T) (=LMG 24555(T)=DSM 21634(T)) is assigned to Pseudorhodoferax soli sp. nov., as the type strain of the type species of the genus. Strain SB1(T) (=LMG 24543(T)=DSM 21598(T)) is the type strain of Pseudorhodoferax caeni sp. nov.
Bruland
Nadine
N
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Correnstrasse 3, 48149 Münster, Germany.
Bathe
Stephan
S
Willems
Anne
A
Steinbüchel
Alexander
A
D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S