title: Absence of Simian virus 40, BK, and JC polyomavirus DNA in squamous cell carcinoma limited to the oral cavity.
abstract: BACKGROUND
  Head and neck squamous cell carcinomas (SCCs) are among the most aggressive types of cancer. The Simian virus 40 (SV40), which is a polyomavirus known for its oncogenic potential, was found as a contaminant of oral vaccines and has been related to human pleomorphic adenoma in the parotid gland. The aim of this study was to evaluate the presence of SV40 and 2 human polyomaviruses-BK virus (BKV) and JC virus (JCV)-in a large sample of SCCs of the oral cavity.
  
  
  METHODS
  Quantitative real-time polymerase chain reaction (PCR) was used to evaluate virus load.
  
  
  RESULTS
  Overall, the prevalence of SV40, BKV, and JCV in oral SCC was negligible. Matched-pair case-control analysis indicated that prevalence among the controls did not significantly differ with respect to analyzed cases.
  
  
  CONCLUSIONS
  The results did not indicate a major role for SV40, BKV, and JCV in the etiology of oral SCC.
authors.affiliation: Department of Histology, Embryology and Applied Biology, Centre of Molecular Genetics, University of Bologna, Bologna, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Palmieri
authors.forename: Francesco
authors.initials: F
authors.lastname: Carinci
authors.forename: Marcella
authors.initials: M
authors.lastname: Martinelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spinelli
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lo Muzio
authors.forename: Corrado
authors.initials: C
authors.lastname: Rubini
authors.forename: Luca
authors.initials: L
authors.lastname: Scapoli
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D016022:Case-Control Studies; D015331:Cohort Studies; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology / Q000821:virology*; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019562:Viral Load
chemical_list: D004269:DNA, Bacterial