Backbone chemical shift assignment of a glutamate receptor ligand binding domain in complexes with five partial agonists.
Backbone (1)H, (13)C, and (15)N chemical shifts are reported for complexes of a perdeuterated glutamate receptor ligand binding domain with kainate, willardiine, and 5-substituted fluoro-, bromo-, and iodowillardiine. These ligands are partial agonists that induce distinct current responses at post-synaptic neurons. The chemical shifts pave the way for numerous NMR studies to identify structural and dynamical determinants of receptor function.
Fenwick
Michael K
MK
Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA.
Oswald
Robert E
RE
D000595:Amino Acid Sequence; D001665:Binding Sites; D002247:Carbon Isotopes / Q000737:chemistry; D018690:Excitatory Amino Acid Agonists / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008970:Molecular Weight; D009587:Nitrogen Isotopes / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011522:Protons; D017470:Receptors, Glutamate / Q000737:chemistry*
D002247:Carbon Isotopes; D018690:Excitatory Amino Acid Agonists; D008024:Ligands; D009587:Nitrogen Isotopes; D011522:Protons; D017470:Receptors, Glutamate