Bone marrow large B cell lymphoma bearing cyclin D3 expression: clinical, morphologic, immunophenotypic, and genotypic analyses of seven patients.
We report seven large B cell lymphoma patients showing the involvement of tumor cells with cyclin D3 (CCND3) expression in bone marrow (BM) at the initial diagnosis. All patients presented with B symptoms, splenomegaly, and anemia/thrombocytopenia lacking hemophagocytosis in the BM. Five of the seven patients had suffered from immunological diseases or cancers. The tumor cells were divided into those with a lymphoplasmacytoid or blastoid appearance. Six cases were confirmed to express CD5 antigen on tumor cells. Three cases presented a chromosomal translocation between CCND3 and the immunoglobulin heavy chain (IGH) loci, t(6;14)(p21;q32). Three and two cases showed unmutated and mutated sequences of the variable region of IGH (VH), respectively, and one case showed deletion of an entire segment of VH. Two cases with t(6;14)(p21;q32) showed an unmutated VH sequence and chromosomal translocation within the switch region of IGH. Further studies are required to determine whether CCND3 expression is associated with a unique subset of diffuse large B cell lymphoma.
Watanuki
Jyuri
J
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
Hatakeyama
Kinta
K
Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Sonoki
Takashi
T
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan. sonoki@wakayama-med.ac.jp.
Tatetsu
Hiro
H
Department of Hematology, Kumamoto University School of Medicine, Kumamoto, Japan.
Yoshida
Katsuhiko
K
Department of Hematology, Saitama Medical University, Saitama, Japan.
Fujii
Soichiro
S
Department of Internal Medicine, Okayama Red Cross General Hospital, Okayama, Japan.
Mizutani
Minoru
M
Department of Internal Medicine, Matsusaka Chuo General Hospital, Matsusaka, Japan.
Abo
Toru
T
Department of Laboratory Medicine, Iwate Medical University, Morioka, Japan.
Kurimoto
Miwa
M
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
Matsuoka
Hiroshi
H
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
Matsuno
Fumihiko
F
Department of Hematology, Kumamoto University School of Medicine, Kumamoto, Japan.
Nakakuma
Hideki
H
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000473:pathology / Q000502:physiology*; D001854:Bone Marrow Cells / Q000473:pathology / Q000502:physiology*; D056743:Cyclin D3; D016213:Cyclins / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D007134:Immunoglobulin Switch Region / Q000235:genetics; D016130:Immunophenotyping; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014178:Translocation, Genetic
C534696:CCND3 protein, human; D056743:Cyclin D3; D016213:Cyclins