title: [Phylogenetic diversity of endophytic endospore-forming bacteria isolated from Cinnamomum longepaniculatum N. Chao ex H. W. Li].
abstract: OBJECTIVE
  In order to study the diversity of endophytic endospore-forming bacteria in Cinnamomum longepaniculatum.
  
  
  METHODS
  We took modified nutrient agar medium for isolation and cultivation and analyzed the 16S rRNA gene sequences of the isolates.
  
  
  RESULTS
  Forty non-redundant endospore-forming bacterial isolates were ascertained, which accounted for 38.1% of all the endophytic bacterial isolates. Of them, 24 isolates were from roots, 7 from stems and 9 from leaves. Phylogenetic analysis based on 16S rRNA gene sequences showed that 35 of them belonged to 16 species of the genera Bacillus, Lysinibacillus and Paenibacillus, and 5 isolates with < 97% sequence similarities to their closely related members were presumed to be potential novel species.
  
  
  CONCLUSIONS
  The results showed that the cultivable endospore-forming bacteria diversity was abundant and there were some potential novel strains in Cinnamomum longepaniculatum. The microflora of endophytic endospore-forming bacteria in individuals of C. longepaniculatum showed that some bacteria distributed in different organs, but the others were organ-specific bacteria.
authors.affiliation: Key Laboratory of Fermentation Resources and Application at Universities of Sichuan Province, Yibin University, Yibin 644000, China. asdfgwt@yahoo.com.cn
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: You
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Cui
authors.forename: Wen
authors.initials: W
authors.lastname: Tian
authors.forename: Naiyao
authors.initials: N
authors.lastname: Huang
authors.forename: Xinglong
authors.initials: X
authors.lastname: Li
mesh_terms: D044822:Biodiversity; D027424:Cinnamomum / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004725:Endospore-Forming Bacteria / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S