title: Hypochlorous acid-induced heme oxygenase-1 gene expression promotes human endothelial cell survival.
abstract: Hypochlorous acid (HOCl) is a unique oxidant generated by the enzyme myeloperoxidase that contributes to endothelial cell dysfunction and death in atherosclerosis. Since myeloperoxidase localizes with heme oxygenase-1 (HO-1) in and around endothelial cells of atherosclerotic lesions, the present study investigated whether there was an interaction between these two enzymes in vascular endothelium. Treatment of human endothelial cells with the myeloperoxidase product HOCl stimulated a concentration- and time-dependent increase in HO-1 protein that resulted in a significant rise in carbon monoxide (CO) production. The induction of HO-1 protein was preceded by a prominent increase in HO-1 mRNA and total and nuclear factor-erythroid 2-related factor 2 (Nrf2). In addition, HOCl induced a significant rise in HO-1 promoter activity that was blocked by mutating the antioxidant response element (ARE) in the promoter or by overexpressing a dominant-negative mutant of Nrf2. The HOCl-mediated induction of Nrf2 or HO-1 was blocked by the glutathione donor N-acetyl-l-cysteine but was unaffected by ascorbic or uric acid. Finally, treatment of endothelial cells with HOCl stimulated mitochondrial dysfunction, caspase-3 activation, and cell death that was potentiated by the HO inhibitor, tin protoporphyrin-IX, or by the knockdown of HO-1, and reversed by the exogenous administration of biliverdin, bilirubin, or CO. These results demonstrate that HOCl induces HO-1 gene transcription via the activation of the Nrf2/ARE pathway to counteract HOCl-mediated mitochondrial dysfunction and cell death. The ability of HOCl to activate HO-1 gene expression may represent a critical adaptive response to maintain endothelial cell viability at sites of vascular inflammation and atherosclerosis.
authors.affiliation: Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri 65212, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wei
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Liu
authors.forename: Kelly J
authors.initials: KJ
authors.lastname: Peyton
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Fruzsina K
authors.initials: FK
authors.lastname: Johnson
authors.forename: Robert A
authors.initials: RA
authors.lastname: Johnson
authors.forename: William
authors.initials: W
authors.lastname: Durante
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002248:Carbon Monoxide / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism* / Q000494:pharmacology; D008665:Metalloporphyrins / Q000494:pharmacology; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000975:Antioxidants; D008665:Metalloporphyrins; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D011524:Protoporphyrins; D012333:RNA, Messenger; D006997:Hypochlorous Acid; D002248:Carbon Monoxide; C032628:tin protoporphyrin IX; D009195:Peroxidase; D051547:Heme Oxygenase-1; D000111:Acetylcysteine