title: Endoscopic ultrasonography (EUS) and EUS-guided fine-needle aspiration with cyst fluid analysis in pancreatic cystic neoplasms.
abstract: OBJECTIVE
  Pancreatic cystic neoplasms represent a heterogeneous group of tumors with varied malignant potential. The aim of this prospective study was to evaluate EUS, EUS-FNA and cyst fluid analysis in distinguishing serous cystadenomas (SCA) and mucinous cystic neoplasms (MCN).
  
  
  METHODS
  Twelve patients with SCA (4 men, 8 women, mean age 58), 16 with MCN (3 men, 13 women, mean age 53) and 10 pancreatic non-tumorous cysts as controls (1 man, 9 women, mean age 43) were investigated by EUS-FNA from January 2003 to February 2006. Cyst fluid evaluation was done for cytology, amylase, CEA, CA 19-9, CA 72-4 and CA 15-3 (RIA). The final diagnosis was based on surgery & histology (14 patients) and/or follow-up after EUS-FNA (mean 15 months).
  
  
  RESULTS
  In the MCN-group 13 mucinous cystadenomas, 2 cystadenocarcinomas and 1 malignant IPMT were found. EUS-FNA results: cytology (including staining for mucin) was diagnostic in 2/12 SCA (17%), 10/16 MCN (63%) and negative in all controls. Fluid CEA in MCN-group (mean 9487, 95%CI 0-23637) was significantly higher compared both with SCA-group (mean 22, 95%CI 0-54, p<0.001) and controls (mean 4, 95%CI 0.5-8, p<0.001). Similar results were found in fluid CA72-4 and fluid CA19-9. Accuracy of EUS-FNA with final diagnosis was 93%.
  
  
  CONCLUSIONS
  EUS-FNA with cyst fluid CEA, CA72-4, CA19-9 and cytology are useful tools in differentiating SCA, MCN and non-tumorous cysts.
authors.affiliation: 2nd Department of Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové University Teaching Hospital, Hradec Králové, Czech Republic. rrepak@seznam.cz
authors.forename: Rudolf
authors.initials: R
authors.lastname: Repák
authors.forename: Stanislav
authors.initials: S
authors.lastname: Rejchrt
authors.forename: Jolana
authors.initials: J
authors.lastname: Bártová
authors.forename: Eva
authors.initials: E
authors.lastname: Malírová
authors.forename: Vera
authors.initials: V
authors.lastname: Tycová
authors.forename: Jan
authors.initials: J
authors.lastname: Bures
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D015331:Cohort Studies; D018291:Cystadenoma, Mucinous / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D018293:Cystadenoma, Serous / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D003937:Diagnosis, Differential; D019160:Endosonography; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010181:Pancreatic Cyst / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012189:Retrospective Studies; D055815:Young Adult