[Immunoenzyme analysis of non-ionic surfactants in water].
The approaches for high sensitive and specific determination of nonylphenol (NP) with the help of immune enzymatic (ELISA) method have been developed. The process of preparation of conjugates of NP with proteins, antiserum obtaining, purification of immunoglobulin (Ig) fractions and study of specificity of the obtained antibodies were described in detail. It was shown that the antiserum and total Ig fraction do not differ in respect of specificity and binding abilities. The developed algorithm of ELISA method fulfillment is able to provide NP revealing with the sensitivity in the range of 20-50 ng/ml and working controlled concentrations--from 20-50 to 1000 ng/ml.
Starodub
N F
NF
Piven'
N V
NV
Demchenko
A V
AV
Goncharik
A V
AV
Orlova
E E
EE
Burakovskiĭ
A I
AI
Mart'ianov
A A
AA
Zherdev
A V
AV
Dzantiev
B B
BB
D000818:Animals; D004784:Environmental Monitoring / Q000379:methods*; D005620:Freund's Adjuvant / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000379:methods*; D010636:Phenols / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D013501:Surface-Active Agents / Q000032:analysis*; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000276:immunology
D007106:Immune Sera; D010636:Phenols; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; D014867:Water; C025256:nonylphenol; D005620:Freund's Adjuvant