A highly sensitive probe detecting low pH area of HeLa cells based on rhodamine B modified beta-cyclodextrins.
Two kinds of rhodamine modified beta-cyclodextrins (R-1 and R-2), which are coupled up ethylene diamine (EDA) and tetraethylene pentamine (TEPA) between Rh B and beta-cyclodextrin, respectively, have been synthesized. R-1 and 2 work as a new fluorogenic probe for monitoring pH of Hela cells, and MTT of assay R-1, R-2, and rhodamine B indicate that less a cytotoxicity of those R-1 and R-2 than that of rhodamine B, where R-1 has much less one than that of R-2. The fluorogenetic probe capability of R-2 was recognized in an area of acidic area in living cell, which is lysosome.
Hasegawa
Takuto
T
Department of Life Science, Akita University, Tegata, Japan.
Kondo
Yoshihiko
Y
Koizumi
Yukio
Y
Sugiyama
Toshihiro
T
Takeda
Akane
A
Ito
Shinichi
S
Hamada
Fumio
F
D045744:Cell Line, Tumor; D005029:Ethylenediamines / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D012235:Rhodamines / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000633:toxicity
D005029:Ethylenediamines; D005456:Fluorescent Dyes; D012235:Rhodamines; D047392:beta-Cyclodextrins; C031234:ethylenediamine; C029773:rhodamine B; C034269:tetraethylenepentamine