[Development in ligase-mediated techniques for bio-molecular analysis].
Two oligonucleotide probes are permitted to anneal to the nucleic acid target of interest so that the ends of two probes immediately become adjacent to each other. The ligase can then efficiently join the two juxtaposed oligonucleotide probes by the formation of a phosphodiester bond if and only if perfectly matched base-pairs at the nick are present. During past 20 years, many ligase-mediated techniques have been developed for analyzing various bio-molecules, such as known/unknown point mutations, small-scale insertions and deletions, CpG islands methylation, large sets of single nucleotide polymorphisms (SNPs), specific proteins and DNA regions with which some other proteins can interact. Since the ligation reaction can be easily integrated into other techniques, certain advances have been already achieved. These novel approaches retain high accuracy through multiple hybridization and enzymatic processing events, and provide inherent quality control checking. In this article, we provide a comprehensive review of the ligase-mediated techniques for bio-molecular analysis.
Xu
Chao
C
State Key Laboratory of Virology, Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Zhou
Yafeng
Y
Zhang
Xian'en
X
D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D008025:Ligases / Q000378:metabolism*; D015336:Molecular Probe Techniques; D009154:Mutation; D015345:Oligonucleotide Probes / Q000096:biosynthesis / Q000138:chemical synthesis / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
D015345:Oligonucleotide Probes; D008025:Ligases