[Analysis of specificity of antiphospholipid antibodies with the use of monoclonal antibodies to phospholipids].
The antigenic specificity of antiphospholipid antibodies (APA) is a matter of intensive investigation. Difference in the reported involvement of APA in clinical manifestation may be due, in part, to the polyclonal nature of these antibodies and the use of serum and serum fractions for analysis. To circumvent this issue, we generated mouse monoclonal APA and compared their antigen binding patterns and conditions of this reaction. Monoclonal APA 5A1 and 1B10 reacted with cardiolipin in a beta2-glycoprotein 1-dependent manner. The epitope for these antibodies consisted of beta2-glycoprotein 1 bound to cardiolipin or immobilized on plastic plates. The specificity is similar to the autoimmune anticardiolipin antibodies described in patients with SLE, APS and other autoimmune diseases. Monoclonal APA 510, 183, 238 reacted with cardiolipin in the absence of beta2-glicoprotein 1. beta2-Glicoprotein 1 , either in the fluid phase or bound to cardiolipin, inhibited the binding of these antibodies. Monoclonal APA 510 was cofactor-independent while monoclonal APA 183 and 238 reacted with cardiolipin only in the presence of human serum. The results of this study indicate that APA comprise a highly heterogeneous population of antibodies with respect to the antigens they recognize, as well as depending on presence of serum components.
Dons'koĭ
B V
BV
Chernyshov
V P
VP
D000818:Animals; D017152:Antibodies, Antiphospholipid / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D002308:Cardiolipins / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D010743:Phospholipids / Q000276:immunology*; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I / Q000276:immunology
D017152:Antibodies, Antiphospholipid; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D002308:Cardiolipins; D010743:Phospholipids; D053482:beta 2-Glycoprotein I