[Establishment of a GFP-based cellular model for screening novel proteasome inhibitors].
To establish a green fluorescent protein (GFP)-based cellular model for screening proteasome inhibitors from compounds including extracts from Traditional Chinese Medicinal herbs, we transfected A549 cells with lentivirus expression vector pGC-E1-ZU1-GFP, and selected clones stably expressing ZU1-GFP. The A549-ZU1-GFP cells were treated with PS-341 for 24 h, and the accumulation of GFP was analyzed by fluorescence microscope. We found that the fluorescence intensity of A549-ZU1-GFP cells treated with PS-341 was significantly increased as compared to the control. We screened for proteasome inhibitors from compounds including some from Traditional Chinese Medicinal herbs, and the data suggested a few compounds could be novel proteasome inhibitors.
Fang
Haitong
H
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China.
Hu
Zheng
Z
Zhou
Guangbiao
G
D001897:Boronic Acids / Q000494:pharmacology; D000069286:Bortezomib; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D011480:Protease Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D011719:Pyrazines / Q000494:pharmacology
D001897:Boronic Acids; D004365:Drugs, Chinese Herbal; D011480:Protease Inhibitors; D011719:Pyrazines; D049452:Green Fluorescent Proteins; D000069286:Bortezomib