[Sequence analysis for the complete proviral genome of avian leukosis virus (subgroup J) strain SD07LK1 isolated from layers].
OBJECTIVE
To study the source and trend in evolution of ALV-J strains from layers in China.


METHODS
The genomic DNA extracted from chicken embryo fibroblasts (CEF) infected by ALV-J strain SD07LK1 isolated from layers was used as template to amplify the ALV-J proviral DNA by PCR. Nine continuous and overlapping fragments were amplified with nine pairs of primers according to published sequences, then cloned into the T vector and sequenced.


RESULTS
The complete sequence of the whole genome of ALV-J Chinese strain SD07LK1 was first established.


CONCLUSIONS
Comparisons of SD07LK1 sequence with that of the other Avian leukosis viruse strains, by using DNAstar software, demonstrated that the genes gag and pol of ALV-J were relatively conservative, the nucleotide identity of all the strains was over 95.0%. However, the gene env identity was only in the ranges between 88.6 and 94.0%.
Guo
Guijie
G
College of Animal Science and Technology,Shandong Agricultural University, Tai'an 271018, China. guojie1125@163.com
Sun
Shuhong
S
Cui
Zhizhong
Z
D000818:Animals; D001353:Avian Leukosis / Q000821:virology*; D001354:Avian Leukosis Virus / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA