Rapid detection of anti-hepatitis A virus neutralizing antibodies in a microplate enzyme immunoassay.
The slow growth of hepatitis A virus (HAV) in cell culture is one of the primary pitfalls in the development of sensitive and rapid methods for the detection and quantification of HAV and associated neutralizing antibodies. Currently, in vitro assays frequently require 8 days or more to detect and quantify the presence of HAV neutralizing antibodies. This study describes a rapid immunoassay that allowed the detection of anti-HAV neutralizing antibodies in only 3 days. This microplate-based enzymic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of HAV vaccines and in quantifying neutralizing antibodies during the course of HAV infection.
Azizi
Ali
A
Department of Pathology and Laboratory Medicine, University of Ottawa, 451 Smyth, Ottawa, ON K1H 8M5, Canada.|Infectious Disease and Vaccine Research Center, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth, Ottawa, ON K1H 8L1, Canada.|Variation Biotechnologies Inc., 1740 Woodroffe Avenue, Building 400, Ottawa, ON K2G 3R8, Canada.
Sirskyj
Danylo
D
Variation Biotechnologies Inc., 1740 Woodroffe Avenue, Building 400, Ottawa, ON K2G 3R8, Canada.
Weltzin
Richard
R
Variation Biotechnologies Inc., 1740 Woodroffe Avenue, Building 400, Ottawa, ON K2G 3R8, Canada.
Anderson
David E
DE
Variation Biotechnologies Inc., 1740 Woodroffe Avenue, Building 400, Ottawa, ON K2G 3R8, Canada.
Diaz-Mitoma
Francisco
F
Department of Pathology and Laboratory Medicine, University of Ottawa, 451 Smyth, Ottawa, ON K1H 8M5, Canada.|Infectious Disease and Vaccine Research Center, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth, Ottawa, ON K1H 8L1, Canada.|Variation Biotechnologies Inc., 1740 Woodroffe Avenue, Building 400, Ottawa, ON K2G 3R8, Canada.
D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006506:Hepatitis A / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D035922:Hepatitis A Antibodies / Q000097:blood*; D022362:Hepatitis A Vaccines / Q000276:immunology; D030041:Hepatitis A virus / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D008253:Macaca mulatta; D009500:Neutralization Tests; D013997:Time Factors; D014776:Virus Cultivation
D035922:Hepatitis A Antibodies; D022362:Hepatitis A Vaccines