Controllable shape selectivity based on highly ordered carbonyl and methyl groups in simple beta-structural polypeptide on silica.
Poly(l-alanine)-grafted porous silica (Sil-Ala(22)) was prepared by polymerization of N-carboxyanhydride of l-alanine initiated by 3-aminopropylated silica. The retention behaviors of the column packed with Sil-Ala(22) were investigated by using alkylbenzenes and polycyclic aromatic hydrocarbons as injection samples in liquid chromatography. The Ala(22) phase was in a rigid beta-form structure and thus provided specific interaction sites, which were derived from the highly ordered carbonyl and methyl groups. These interaction sites bring unique molecular-shape discriminations: molecular-length and non-planarity selectivity, which are controllable by altering organic solvent used as a part of mobile phase.
Shundo
Atsuomi
A
Department of Applied Chemistry and Biochemistry, Kumamoto University, Kumamoto 860-8555, Japan.
Mallik
Abul K
AK
Sakurai
Toshihiko
T
Takafuji
Makoto
M
Nagaoka
Shoji
S
Ihara
Hirotaka
H
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D008958:Models, Molecular; D010455:Peptides; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000302:isolation & purification*; D017433:Protein Structure, Secondary; D012822:Silicon Dioxide
D010455:Peptides; D011084:Polycyclic Aromatic Hydrocarbons; C019529:polyalanine; D012822:Silicon Dioxide