title: The dynamics of individual nucleosomes controls the chromatin condensation pathway: direct atomic force microscopy visualization of variant chromatin.
abstract: Chromatin organization and dynamics is studied at scales ranging from single nucleosome to nucleosomal array by using a unique combination of biochemical assays, single molecule imaging technique, and numerical modeling. We show that a subtle modification in the nucleosome structure induced by the histone variant H2A.Bbd drastically modifies the higher order organization of the nucleosomal arrays. Importantly, as directly visualized by atomic force microscopy, conventional H2A nucleosomal arrays exhibit specific local organization, in contrast to H2A.Bbd arrays, which show "beads on a string" structure. The combination of systematic image analysis and theoretical modeling allows a quantitative description relating the observed gross structural changes of the arrays to their local organization. Our results suggest strongly that higher-order organization of H1-free nucleosomal arrays is determined mainly by the fluctuation properties of individual nucleosomes. Moreover, numerical simulations suggest the existence of attractive interactions between nucleosomes to provide the degree of compaction observed for conventional chromatin fibers.
authors.affiliation: Université de Lyon, Laboratoire Joliot-Curie, CNRS USR 3010, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 07, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Montel
authors.forename: Hervé
authors.initials: H
authors.lastname: Menoni
authors.forename: Martin
authors.initials: M
authors.lastname: Castelnovo
authors.forename: Jan
authors.initials: J
authors.lastname: Bednar
authors.forename: Stefan
authors.initials: S
authors.lastname: Dimitrov
authors.forename: Dimitar
authors.initials: D
authors.lastname: Angelov
authors.forename: Cendrine
authors.initials: C
authors.lastname: Faivre-Moskalenko
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014981:Xenopus
chemical_list: D006657:Histones; D009707:Nucleosomes; D011993:Recombinant Fusion Proteins