Sequence and gene organization of 24 circles from the Cotesia plutellae bracovirus genome.
Twenty-four genomic segments of Cotesia plutellae bracovirus (CpBV) were completely sequenced, and their genomic structures were analyzed. The aggregated genome size is 351,299 bp long and exhibits an average GC content of approximately 34.6%. Average coding density is about 32.3%, and 125 putative open reading frames (ORFs) are predicted. More than half (52.5%) of predicted genes are annotated as hypothetical, but they share sequence similarities with those of other bracoviral genomes. The annotated ORFs can be classified into the known bracoviral families, in which a family of protein tyrosine phosphatase is the largest, including 36 ORFs, suggesting a significant role during parasitization. In addition, 8 and 7 ORFs encode ankyrin-like and EP1-like genes, respectively. Some predicted genes are known only in Cotesia-associated bracoviral genomes. Phylogenetic analyses based on PTP, ankyrin and EP1-like gene groups revealed no correlation between bracoviruses.
Choi
Jae Young
JY
Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
Kwon
Soo-Jin
SJ
Roh
Jong Yul
JY
Yang
Tae Jin
TJ
Yoon
Sook Hee
SH
Kim
Heebal
H
Li
Ming Shun
MS
Park
Beom-Seok
BS
Woo
Soo-Dong
SD
Jin
Byung Rae
BR
Kim
Yonggyun
Y
Je
Yeon Ho
YH
D000818:Animals; D017487:Ankyrins / Q000235:genetics; D001483:Base Sequence; D023061:Gene Order; D005814:Genes, Viral; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D006927:Hymenoptera / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D018071:Polydnaviridae / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D017487:Ankyrins; D006023:Glycoproteins; D014764:Viral Proteins; D017027:Protein Tyrosine Phosphatases