[Molecular classification of a phytoplasma associated with willow yellow disease].
OBJECTIVE
Willow Yellow is a plant disease caused by phytoplasma. We identified the taxonomic status of Willow Yellow phytoplasma (WY).


METHODS
We used specific conservative primers amplified 16S rDNA and ribosomal proteins gene (rp) gene. Obtained sequences were then analyzed and homology trees were constructed. The nested-PCR products were also analyzed by restriction fragment length polymorphism (RFLP).


RESULTS
We obtained 16S rDNA of 1246 bp and rp gene of 1212 bp, and sequence analysis showed a high identity with members of the 16SrI-C group phytoplasmas, all above 99.0%, and had the highest identity with Wheat Blue Dwarf phytoplasmas, 99.8% (16S rDNA) and 99.6% (rp) respectively, RFLP pattern are also the same as 16S rI-C phytoplsama.


CONCLUSIONS
Willow yellow phytoplasma is a member of 16S rI-C.
Wei
Ting
T
College of Plant Protection and Shaanxi Key Lab of Mol Biol for Agriculture, Key Lab of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech Univ of Agriculture and Forestry, Yangling , China. weiting029@yahoo.cn
Wu
Yunfeng
Y
Hou
Wei
W
Wu
Keke
K
Li
Yiran
Y
Zhang
Jue
J
Sun
Runhong
R
D004275:DNA, Ribosomal / Q000235:genetics; D045582:Phytoplasma / Q000145:classification* / Q000235:genetics*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D032108:Salix / Q000382:microbiology*
D004275:DNA, Ribosomal