[Effects of placental growth factor on the secretion of proinflammatory cytochemokines in vascular endothelial cells].
OBJECTIVE
Placental growth factor (PIGF) mediates angiogenesis and inflammation, and is up-regulated in early and advanced atherosclerotic lesions. But its effects on the function of vascular endothelial cells is still largely unknown. This study was designed to test the effects of PIGF on the secretion of proinflammatory cytochemokines in vascular endothelial cells.


METHODS
Cultured human umibilical vein endotheial cells (HUVECs) of passages 3-5 were used. Growth-arrested HUVECs were treated with 5, 10, 25 or 50 ng/mL recombinant human PIGF (rhPLGF) for 4, 8, 24 or 48 hours. Monocyte chemotactic protein-1 (MCP-1), soluble vascular cell adhesion molecule-1 (sVCAM-1) and P-selectin secreted from HUVECs were measured by ELISA.


RESULTS
Treated with rhPIGF at 10-50 ng/mL for 48 h, the production of MCP-1 from the HUVECs increased significantly. Treatment with rhPIGF at 5-50 ng/mL for 4 h, the release of P-selectin from the HUVECs increased significantly. But rhPIGF (5-50 ng/mL) had no effect on sVCAM secretion.


CONCLUSIONS
Exogenous PIGF could modulate the secretion function of vascular. endothelial cells, stimulating MCP-1 and P-selectin release in HUVECs.
Fu
Hua
H
Laboratory of Cardiovascular Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
Wu
Wen-chao
WC
Hu
Hong-de
HD
Liu
Xiao-jing
XJ
D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D019007:P-Selectin / Q000096:biosynthesis / Q000235:genetics; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D016207:Cytokines; D019007:P-Selectin; C000608036:PGF protein, human; D011257:Pregnancy Proteins; D019010:Vascular Cell Adhesion Molecule-1; D000072483:Placenta Growth Factor