Optimisation of Dex-GMA nanoparticles prepared in modified micro-emulsion system: physical and biologic characterization.
In recent years, utilizing nanoparticles as delivery system for drug targeting delivery has raised increasing interest. In this study, glycidyl methacrylate derivatized dextran (Dex-GMA) nanoparticles encapsulating basic fibroblast growth factor (bFGF) have been prepared inside the aqueous cores of sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/n-hexane reverse micelles. These nanoparticles were identified to be uniformly spherical in shape with an average size of 109.57+/-2.09 nm. And 90.2% of the nanoparticles were in a narrow size range of 80-110 nm. The release of bFGF from the nanoparticles is completely and sustained as long as 35 days. The impact of the nanoparticles on mouse bone marrow mesenchymal stem cells (BMSCs) was assessed with cell cytotoxicity/viability and adhesion assay. Those studies show that the Dex-GMA nanoparticles prepared by water-in-oil micro-emulsion systems with aprotic solvent adding are novel, effective and biocompatible delivery system for bioactive protein.
Wu
Xiaochen
X
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Xi'an 710032, China.
Wei
Xufeng
X
Gu
Chunhu
C
Chen
Yu
Y
Wu
Hong
H
Zhang
Jinbao
J
Yi
Dinghua
D
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry* / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000493:pharmacokinetics; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008823:Micelles; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*
D003911:Dextrans; D004655:Emulsions; D008689:Methacrylates; D008823:Micelles; D016222:Fibroblast Growth Factor 2