HAT-HDAC interplay modulates global histone H3K14 acetylation in gene-coding regions during stress.
Histone acetylation and deacetylation are important for gene regulation. The histone acetyltransferase, Gcn5, is an activator of transcriptional initiation that is recruited to gene promoters. Here, we map genome-wide Gcn5 occupancy and histone H3K14ac at high resolution. Gcn5 is predominantly localized to coding regions of highly transcribed genes, where it collaborates antagonistically with the class-II histone deacetylase, Clr3, to modulate H3K14ac levels and transcriptional elongation. An interplay between Gcn5 and Clr3 is crucial for the regulation of many stress-response genes. Our findings suggest a new role for Gcn5 during transcriptional elongation, in addition to its known role in transcriptional initiation.
Johnsson
Anna
A
Department of Biosciences and Medical Nutrition, Karolinska Institute, S-141 57 Huddinge, Sweden.
Durand-Dubief
Mickaël
M
Xue-Franzén
Yongtao
Y
Rönnerblad
Michelle
M
Ekwall
Karl
K
Wright
Anthony
A
D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones; D016366:Open Reading Frames; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014158:Transcription, Genetic; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D018797:Cell Cycle Proteins; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins; C115302:Clr3 protein, S pombe; C501322:Gcn5 protein, S pombe; D000099224:p300-CBP-Associated Factor