[Impact of PrLZ overexpression on invasion of prostate cancer LNCaP cells in vitro].
OBJECTIVE
Prostate leucine zipper (PrLZ), a novel gene, is highly associated with the malignant progression of prostate cancer. We previously found that PrLZ overexpression can enhance the survival of prostate cancer LNCaP cells in vitro. This study was to investigate the impact of PrLZ on the invasion of LNCaP cells and its possible mechanism.


METHODS
PrLZ was transfected into LNCaP cells. LNCaP/PrLZ cells with high expression of PrLZ were screened with G418. The migration and invasion of LNCaP and LNCaP/PrLZ cells were detected by Transwell assay. The level and activity of matrix metalloproteinase-2 (MMP-2) was determined by Western blot and Zymography, respectively.


RESULTS
Migration cell count of LNCaP and LNCaP/PrLZ cells were 64.8+/-8.7 and 69.3+/-7.6, respectively (P>0.05), whereas invasive cell count of LNCaP and LNCaP/PrLZ cells were 70.7+/-2.8 and 190.5+/-9.3, respectively (P<0.001). Both the expression and activity of MMP-2 in LNCaP/PrLZ cells were enhanced.


CONCLUSIONS
PrLZ may be involved in the invasion of prostate cancer.
Zhang
Dong
D
Department of Urology, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Li
Lei
L
Zhang
Lin-Lin
LL
Xue
Yan
Y
Wang
Xin-Yang
XY
He
Da-Lin
DL
D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
D009363:Neoplasm Proteins; C114751:TPD52 protein, human; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2