[Structure-functional analysis of interactions of terminal deoxynucleotidyl transferase with new non-nucleoside substrates].
New non-nucleoside esters of phosphoric acid containing various hydrophobic groups, namely (1) N-(2-tripticencarbonyl)-4-aminobutyl; (2) 5-phenylsubstituted N-(2,4-dinitrophenyl)-4-aminobutyl; (3) N-(4-phenylbenzoyl)- and N-(4-(N-benzylamino)benzoyl)-2-aminoethyl groups, as well as (4) diphenylmethyl and fluorenyl groups were synthesized and studied as substrates of terminal deoxynucleotidyl transferase. With the exception of the two latter derivatives, all the analogues displayed substrate properties and could incorporate into the deoxyoligonucleotide 3'-end. As it was shown in biochemical experiments and by computer modeling, a linker joining the triphosphate and hydrophobic fragments of the molecule was necessary for these compounds to display substrate properties.
Matyugina
E S
ES
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov ul. 32, Moscow, 119991 Russia.
Alexandrova
L A
LA
Jas'ko
M V
MV
Ivanov
A V
AV
Vasil'ev
I A
IA
Lapteva
V L
VL
Khandazhinskaya
A L
AL
Kukhanova
M K
MK
D020134:Catalytic Domain; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry*; D004952:Esters; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011122:Polyphosphates / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D004952:Esters; D009838:Oligodeoxyribonucleotides; D011122:Polyphosphates; D004253:DNA Nucleotidylexotransferase; C005692:triphosphoric acid