title: The male sterility locus ms3 is present in a fertility controlling gene cluster in soybean.
abstract: Soybean [Glycine max (L.) Merr.] is self-pollinated. To produce large quantities of hybrid seed, insect-mediated cross-pollination is necessary. An efficient nuclear male-sterile system for hybrid seed production would benefit from molecular and/or phenotypic markers linked to male fertility/sterility loci to facilitate early identification of phenotypes. Nuclear male-sterile, female-fertile ms3 mutant is a single recessive gene and displays high outcrossed seed set with pollinators. Our objective was to map the ms3 locus. A segregating population of 150 F(2) plants from Minsoy (PI 27890) x T284H, Ms3ms3 (A00-68), was screened with 231 simple sequence repeat markers. The ms3 locus mapped to molecular linkage group (MLG) D1b (Gm02) and is flanked by markers Satt157 and Satt542, with a distance of 3.7 and 12.3 cM, respectively. Female-partial sterile-1 (Fsp1) and the Midwest Oilseed male-sterile (msMOS) mutants previously were located on MLG D1b. msMOS and Fsp1 are independent genes located very close to each other. All 3 genes are located in close proximity of Satt157. We believe that this is the first report of clustering of fertility-related genes in plants. Characterization of these closely linked genes may help in understanding the evolutionary relationship among them.
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA.
authors.forename: Innan
authors.initials: I
authors.lastname: Cervantes-Martinez
authors.forename: Devinder
authors.initials: D
authors.lastname: Sandhu
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Evelyn
authors.initials: E
authors.lastname: Ortiz-Pérez
authors.forename: Kiyoaki K
authors.initials: KK
authors.lastname: Kato
authors.forename: Harry T
authors.initials: HT
authors.lastname: Horner
authors.forename: Reid G
authors.initials: RG
authors.lastname: Palmer
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D017343:Genes, Plant / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*