title: [Expression of angiogenic factors in pancreatic carcinoma cell and their significance].
abstract: OBJECTIVE
  To investigate the relationship between expression of angiogenic factors and invasion and proliferation in pancreatic carcinoma cell.
  
  
  METHODS
  Expressions and secretions of angiogenic factors in pancreatic carcinoma cell lines were determined by RT-PCR and ELISA. Proliferation and invasion of pancreatic carcinoma cell lines were determined by CCK-8 and Boyden Chamber invasion tests.
  
  
  RESULTS
  Pancreatic carcinoma cell lines showed significantly different ability of invasion and proliferation. Intracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (152.9 +/- 0.6), (224.1 +/- 60.3), (239.2 +/- 2.1), (19.3 +/- 0.7), (165.6 +/- 34.3), and (18.1 +/- 1.4) pg/(10(6)cell.24 h). Extracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (1331.1 +/- 67.8), (3902.6 +/- 79.7), (2657.3 +/- 51.9), (1498.3 +/- 4.8), (4696.8 +/- 45.5), and (1200.5 +/- 42.2) pg/(10(6)cell.24 h). Intracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (66.1 +/- 4.8), (206.8 +/- 99.5), (1532.0 +/- 54.6), (159.2 +/- 11.0), (1612.0 +/- 515.9) and (2781.2 +/- 479.0) pg/(10(6)cell.24 h). Extracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (2.1 +/- 0.6), (10.3 +/- 1.5), (31.0 +/- 0.4), (4.3 +/- 1.2), (43.6 +/- 1.5) and (82.1 +/- 10.4) pg/(10(6)cell.24 h). Intracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.2 +/- 0.0), (0.3 +/- 0.0), (4.7 +/- 0.1), (10.8 +/- 0.2), (31.9 +/- 11.7) and (5.4 +/- 0.1) ng/(10(6)cell.24 h). Extracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.0 +/- 0.0), (1.6 +/- 0.0), (21.5 +/- 1.1), (40.8 +/- 0.4), (129.2 +/- 1.0) and (20.1 +/- 1.8) ng/(10(6)cell.24 h). Panc-1 enjoying stronger invasion and proliferation showed stronger expressions of VEGF, bFGF and endostatin. Intracellular expressions of bFGF was stronger than extracellular, extracellular expressions of VEGF and endostatin were stronger than intracellular.
  
  
  CONCLUSIONS
  Expressions of angiogenic factors regulated by cancer cell played an important role in progression of pancreatic carcinoma.
authors.affiliation: Department of Hepatobiliary Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050011, China.
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Yan
authors.forename: Yu-pei
authors.initials: YP
authors.lastname: Zhao
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D043169:Endostatins / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D043169:Endostatins; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2