title: Immunoglobulin E cross-reactivity between lupine conglutins and peanut allergens in serum of lupine-allergic individuals.
abstract: BACKGROUND
  Lupine is used increasingly in food products. The development of lupine allergy in peanut-allergic patients is believed to occur as a result of cross-reactivity between lupine and peanut proteins.
  
  
  OBJECTIVE
  To investigate the degree of immunoglobulin (Ig) E cross-reactivity between allergens in lupine and peanut.
  
  
  METHODS
  We investigated IgE cross-reactivity between lupine alpha-, beta-, gamma-, and delta-conglutins and the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 using enzyme-linked immunosorbent assay with sera from patients with coexisting peanut and lupine allergy.
  
  
  RESULTS
  Peanut proteins inhibited IgE binding towards alpha- conglutins, delta-conglutins, and, to a lesser degree, beta-conglutins, while no IgE cross-reaction with delta-conglutin was observed. Ara h 2 most potently inhibited IgE binding to lupine and delta-conglutins, while Ara h 1 most potently cross-reacted with beta-conglutin. Ara h 3 was apparently not involved in these mechanisms.
  
  
  CONCLUSIONS
  The present study reveals IgE cross-reactivity between the 2S albumins Ara h 2 and delta-conglutin, and the 7S vicilin-like Ara h 1 and beta-conglutin, which are possibly based on homologies between phylogenetically related proteins. Ara h 2 was the most potent inhibitor of IgE binding to lupine conglutins.
authors.affiliation: National Veterinary Institute, Oslo, Norway.
authors.forename: M M B W
authors.initials: MM
authors.lastname: Dooper
authors.forename: C
authors.initials: C
authors.lastname: Plassen
authors.forename: L
authors.initials: L
authors.lastname: Holden
authors.forename: H
authors.initials: H
authors.lastname: Lindvik
authors.forename: C K
authors.initials: CK
authors.lastname: Faeste
mesh_terms: D055516:2S Albumins, Plant / Q000276:immunology; D000293:Adolescent; D000485:Allergens / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology; D010367:Arachis / Q000276:immunology*; D001667:Binding, Competitive; D002648:Child; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D031297:Lupinus / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins; D021183:Peanut Hypersensitivity / Q000097:blood / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology; D055314:Seed Storage Proteins / Q000276:immunology; D017386:Sequence Homology, Amino Acid
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; C500890:Ara h 2 allergen, Arachis hypogaea; D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; D055314:Seed Storage Proteins; C118207:allergen Ara h3; C042457:conglutin protein, Lupinus angustifolius; D007073:Immunoglobulin E