title: Andreprevotia lacus sp. nov., isolated from a fish-culture pond.
abstract: A bacterial strain designated GFC-1(T) was isolated from a fish-culture pond in Taiwan and was characterized by using the polyphasic taxonomic approach. Strain GFC-1(T) was Gram-negative, aerobic, rod-shaped, motile and non-spore-forming. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Andreprevotia of the family Neisseriaceae and its closest neighbour was Andreprevotia chitinilytica JS11-7(T) (97.0 % sequence similarity). The results of physiological and biochemical tests allowed clear phenotypic differentiation of isolate GFC-1(T) from A. chitinilytica JS11-7(T). The major fatty acids were summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(18 : 1)omega7c. The DNA G+C content was 63.0 mol%. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and physiological data, strain GFC-1(T) should be classified as representing a novel species and a second member of the genus Andreprevotia, for which the name Andreprevotia lacus sp. nov. is proposed. The type strain is GFC-1(T) (=BCRC 17832(T)=LMG 24502(T)).
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.forename: Tzu Fang
authors.initials: TF
authors.lastname: Chiu
authors.forename: Jui-Hsing
authors.initials: JH
authors.lastname: Chou
authors.forename: Der-Shyan
authors.initials: DS
authors.lastname: Sheu
authors.forename: A B
authors.initials: AB
authors.lastname: Arun
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.forename: Chaolun Allen
authors.initials: CA
authors.lastname: Chen
authors.forename: Jih-Terng
authors.initials: JT
authors.lastname: Wang
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005399:Fishes; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S