title: Endothelial colony-forming cells from patients with chronic myeloproliferative disorders lack the disease-specific molecular clonality marker.
abstract: Two putative types of circulating endothelial progenitor cells have been recently identified in vitro: (1) endothelial colony-forming cell (ECFC) and (2) colony-forming unit-endothelial cell (CFU-EC). Only the former is now recognized to belong to endothelial lineage. We have used the ECFC and CFU-EC assays to readdress the issue of the clonal relation between endothelial progenitor cells and hematopoietic stem cells in patients with Philadelphia-positive and Philadelphia-negative chronic myeloproliferative disorders. Both ECFCs and CFU-ECs were cultured from peripheral blood mononuclear cells, and either BCR-ABL rearrangement or JAK2-V617F mutation were assessed in both types of endothelial colonies. We found that ECFCs lack the disease-specific markers, which are otherwise present in CFU-ECs, thus reinforcing the concept that the latter belongs to the hematopoietic lineage, and showing that in chronic myeloproliferative disorders the cell that gives rise to circulating ECFC has a distinct origin from the cell of the hematopoietic malignant clone.
authors.affiliation: Divisione Ematologia, Ospedale San Martino, Genova.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Piaggio
authors.forename: Vittorio
authors.initials: V
authors.lastname: Rosti
authors.forename: Mirko
authors.initials: M
authors.lastname: Corselli
authors.forename: Francesca
authors.initials: F
authors.lastname: Bertolotti
authors.forename: Gaetano
authors.initials: G
authors.lastname: Bergamaschi
authors.forename: Sarah
authors.initials: S
authors.lastname: Pozzi
authors.forename: Davide
authors.initials: D
authors.lastname: Imperiale
authors.forename: Barbara
authors.initials: B
authors.lastname: Chiavarina
authors.forename: Elisa
authors.initials: E
authors.lastname: Bonetti
authors.forename: Francesca
authors.initials: F
authors.lastname: Novara
authors.forename: Mario
authors.initials: M
authors.lastname: Sessarego
authors.forename: Laura
authors.initials: L
authors.lastname: Villani
authors.forename: Anna
authors.initials: A
authors.lastname: Garuti
authors.forename: Margherita
authors.initials: M
authors.lastname: Massa
authors.forename: Riccardo
authors.initials: R
authors.lastname: Ghio
authors.forename: Rita
authors.initials: R
authors.lastname: Campanelli
authors.forename: Andrea
authors.initials: A
authors.lastname: Bacigalupo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pecci
authors.forename: Gianluca
authors.initials: G
authors.lastname: Viarengo
authors.forename: Orsetta
authors.initials: O
authors.lastname: Zuffardi
authors.forename: Francesco
authors.initials: F
authors.lastname: Frassoni
authors.forename: Giovanni
authors.initials: G
authors.lastname: Barosi
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002478:Cells, Cultured; D002908:Chronic Disease; D003114:Colony-Forming Units Assay; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000172:deficiency* / Q000235:genetics; D015321:Gene Rearrangement; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D053614:Janus Kinase 2 / Q000172:deficiency* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D009196:Myeloproliferative Disorders / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D013234:Stem Cells / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D016044:Fusion Proteins, bcr-abl; C507924:JAK2 protein, human; D053614:Janus Kinase 2