Improved method for assessing iron stores in the bone marrow.
BACKGROUND
Bone marrow iron microscopy has been the "gold standard" method of assessing iron deficiency. However, the commonly used method of grading marrow iron remains highly subjective.


OBJECTIVE
To improve the bone marrow grading method by developing a detailed protocol that assesses iron in fragments, in macrophages around fragments and in erythroblasts.


METHODS
A descriptive study of marrow aspirates of 303 children (aged 6-60 months) with severe anaemia and 22 controls (children undergoing elective surgery) was conducted at hospitals in southern Malawi (2002-04).


RESULTS
Using an intensive marrow iron grading method, 22% and 39% of cases and controls had deficient iron stores, and 40% and 46% had functional iron deficiency, respectively. Further evaluation of the iron status classification by the intensive method showed that functional iron deficiency was associated with significantly increased C-reactive protein concentrations (126.7 (85.6) mg/l), and iron stores deficiency with significantly increased soluble transferrin receptor concentrations (21.7 (12.5) mug/ml).


CONCLUSIONS
Iron assessment can be greatly improved by a more intense marrow examination. This provides a useful iron status classification which is of particular importance in areas where there is a high rate of inflammatory conditions.
Phiri
K S
KS
Malawi-Liverpool-Wellcome Trust Clinical Research Programme, College of Medicine, PO Box 30096, Blantyre 3, Malawi. kamijaphiri@gmail.com
Calis
J C J
JC
Kachala
D
D
Borgstein
E
E
Waluza
J
J
Bates
I
I
Brabin
B
B
van Hensbroek
M Boele
MB
D018798:Anemia, Iron-Deficiency / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D001853:Bone Marrow / Q000737:chemistry; D001856:Bone Marrow Examination / Q000379:methods*; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007501:Iron / Q000032:analysis; D008297:Male; D011990:Receptors, Transferrin / Q000097:blood; D012720:Severity of Illness Index
D015415:Biomarkers; D011990:Receptors, Transferrin; D002097:C-Reactive Protein; D007501:Iron