title: Accurate quantitation of standard peptides used for quantitative proteomics.
abstract: MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.
authors.affiliation: Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, USA.
authors.forename: Narisa K
authors.initials: NK
authors.lastname: Bordeerat
authors.forename: Nadia I
authors.initials: NI
authors.lastname: Georgieva
authors.forename: David G
authors.initials: DG
authors.lastname: Klapper
authors.forename: Leonard B
authors.initials: LB
authors.lastname: Collins
authors.forename: Tyra J
authors.initials: TJ
authors.lastname: Cross
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
authors.forename: James A
authors.initials: JA
authors.lastname: Swenberg
authors.forename: Gunnar
authors.initials: G
authors.lastname: Boysen
mesh_terms: D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods; D010455:Peptides / Q000032:analysis*; D040901:Proteomics / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods
chemical_list: D010455:Peptides