[Molecular interaction between PrP protein and the signal protein 14-3-3 beta].
The molecular interaction between PrP and 14-3-3 beta and the possible interactional domain between two proteins were studied by co-immunoprecipitation, pull down and FRET assays. The results showed that PrP protein could interact with 14-3-3 beta in vitro and in vivo. The domain which responded for the interaction was located at C-terminal of PrP (amino acid residues 106 to 126). This study of the interaction between PrP and 14-3-3 protein further provided the insight into the potential role of 14-3-3 in the biological function of PrP and the pathogenesis of prion disease.
Mei
Guo-yong
GY
Pathogenic Microorganisms Department, Medical School, Anhui University of Science and Technology, Huainan, 232001, China. 06mgy@163.com
Li
Yuan
Y
Wang
Gui-rong
GR
Zhang
Bao-yun
BY
Tian
Chan
C
Chen
Cao
C
Zhou
Rui-min
RM
Wang
Xin
X
Li
Xiao-li
XL
Wang
Ke-xia
KX
Han
Jun
J
Dong
Xiao-ping
XP
D048948:14-3-3 Proteins / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D006224:Cricetinae; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D011328:Prions / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits
D048948:14-3-3 Proteins; D011328:Prions