Helcobacillus massiliensis gen. nov., sp. nov., a novel representative of the family Dermabacteraceae isolated from a patient with a cutaneous discharge.
Gram-positive, non-spore-forming rods (strain 6401990T), isolated from a human cutaneous discharge were subjected to a polyphasic taxonomy study. The only respiratory quinone was MK-7 and the major fatty acids were anteiso-C15:0 (34.3%), anteiso-C17:0 (18.7%) and iso-C16:0 (18.6%). Mycolic acids were not present. Polar lipids present were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and unidentified glycolipids. The isomer of diaminopimelic acid identified was meso-diaminopimelic acid, and the analysis of whole-cell sugars showed the presence of high amounts of galactose, ribose and some glucose. The G+C content of strain 6401990T was 68.6%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed 95.1% similarity with Dermabacter hominis. On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain represents a novel species in a new genus of the family Dermabacteraceae, for which the name Helcobacillus massiliensis gen. nov., sp. nov. is proposed. The type strain is 6401990T (CSUR P17T=CIP 109418T=CCUG 53859T).
Renvoise
Aurélie
A
Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, CNRS UMR 6236, CNRS-IRD, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
Aldrovandi
Nicolas
N
Raoult
Didier
D
Roux
Véronique
V
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012266:Ribose / Q000032:analysis; D017422:Sequence Analysis, DNA; D017192:Skin Diseases, Bacterial / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D009171:Mycolic Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D012266:Ribose; D005947:Glucose; D005690:Galactose