title: Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells.
abstract: BACKGROUND
  Matrine (MT) is a main active ingredient of Sophora flavescens roots, which is used in Traditional Chinese Medicine (TCM) for the treatment of inflammations like enteritis, hepatitis and atopic dermatitis.
  
  
  OBJECTIVE
  Aim of the study is to gain insight into the effects of MT on nitric oxide (NO) release, intracellular NO production, and endothelial nitric oxide synthase (eNOS) level in second generation rat intestinal microvascular endothelial cells (RIMECs). Moreover, the effects of MT on soluble intercellular adhesion molecule-1 (sICAM-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) production induced by lipopolysaccharide (LPS) in these cells were evaluated.
  
  
  METHODS
  Isolated and identified RIMECs cultures were exposed to different concentrations of matrine, and changes in extra- and intracellular NO concentrations were measured in dependance of time by Griess reaction or DAF-FM diacetate. Obtained cell cultures were solitude treated with lypopolysaccharide (LPS) or combined with MT to observe impacts on sICAM-1, IL-6 and IL-8 concentration in culture supernatants by ELISA.
  
  
  RESULTS
  Matrine dose-dependently increased the concentration of NO in culture supernatant of RIMECs. Exposure of MT resulted in a steady intracellular NO increase pattern under different concentrations with different values and has an increasing effect on eNOS concentration at a long time exposure. Additionally, matrine reduced the increasing effect of LPS on the production of IL-6, IL-8, and sICAM-1 in RIMECs.
  
  
  CONCLUSIONS
  These results show that matrine may serve as a protective agent against tissue damage in inflammation by improving NO-dependent vasomotion and inhibiting inflammatory cytokines induced by LPS.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Suo
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Miro
authors.initials: M
authors.lastname: Ferreri
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Mu
authors.forename: Bo
authors.initials: B
authors.lastname: Han
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000098:blood supply; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000201:enzymology; D004730:Endothelium, Vascular / Q000187:drug effects; D005260:Female; D018836:Inflammation Mediators / Q000737:chemistry / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007422:Intestines / Q000098:blood supply / Q000187:drug effects*; D007583:Jejunum / Q000098:blood supply*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008833:Microcirculation; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D011247:Pregnancy; D011807:Quinolizines / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D029909:Sophora / Q000737:chemistry; D013997:Time Factors; D000093842:Matrines
chemical_list: D000470:Alkaloids; D016895:Culture Media, Serum-Free; D018836:Inflammation Mediators; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011807:Quinolizines; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; D000093842:Matrines