title: Overexpression of Mn superoxide dismutase does not increase life span in mice.
abstract: Genetic manipulations of Mn superoxide dismutase (MnSOD), SOD2 expression have demonstrated that altering the level of MnSOD activity is critical for cellular function and life span in invertebrates. In mammals, Sod2 homozygous knockout mice die shortly after birth, and alterations of MnSOD levels are correlated with changes in oxidative damage and in the generation of mitochondrial reactive oxygen species. In this study, we directly tested the effects of overexpressing MnSOD in young (4-6 months) and old (26-28 months) mice on mitochondrial function, levels of oxidative damage or stress, life span, and end-of-life pathology. Our data show that an approximately twofold overexpression of MnSOD throughout life in mice resulted in decreased lipid peroxidation, increased resistance against paraquat-induced oxidative stress, and decreased age-related decline in mitochondrial ATP production. However, this change in MnSOD expression did not alter either life span or age-related pathology.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78245-3207, USA.
authors.forename: Youngmok C
authors.initials: YC
authors.lastname: Jang
authors.forename: Viviana I
authors.initials: VI
authors.lastname: Pérez
authors.forename: Wook
authors.initials: W
authors.lastname: Song
authors.forename: Michael S
authors.initials: MS
authors.lastname: Lustgarten
authors.forename: Adam B
authors.initials: AB
authors.lastname: Salmon
authors.forename: James
authors.initials: J
authors.lastname: Mele
authors.forename: Wenbo
authors.initials: W
authors.lastname: Qi
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Hanyu
authors.initials: H
authors.lastname: Liang
authors.forename: Asish
authors.initials: A
authors.lastname: Chaudhuri
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikeno
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Epstein
authors.forename: Holly
authors.initials: H
authors.lastname: Van Remmen
authors.forename: Arlan
authors.initials: A
authors.lastname: Richardson
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008931:Mitochondria, Muscle / Q000502:physiology; D018482:Muscle, Skeletal / Q000473:pathology; D009929:Organ Size; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000502:physiology*; D000099156:Superoxide Dismutase 2
chemical_list: D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2