ChIPing away at the genome: the new frontier travel guide.
Chromatin immunoprecipitation (ChIP) is a powerful technique for obtaining in vivo data on protein-DNA binding, providing an invaluable tool for elucidating gene regulation at a molecular level. Combined with high-throughput methods such as microarrays (ChIP-array) and second generation sequencing (ChIP-seq), the technique is now commonly used for answering questions about protein binding on a genome-wide level. This review focuses on the use of microarrays and sequencing for ChIP studies, provides a critical comparison of the currently used platforms and an overview of the computational methods available, and offers recommendations for optimal use of the techniques in a research context.
Aleksic
Jelena
J
Department of Genetics and Cambridge Systems Biology Centre, University of Cambridge, Downing Street, Cambridge, UK.
Russell
Steven
S
D047369:Chromatin Immunoprecipitation; D023281:Genomics; D020411:Oligonucleotide Array Sequence Analysis