[Reparative isolation and purification of flavones from Pericarpium Citri Reticulatae by high-speed counter-current chromatography].
Three flavones were prepared, isolated and purified from Pericarpium Citri Reticulatae by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water (2:4:3:3, v/v/v/v) was used. Within 6 hours, 10.1 mg of heseridin, 49.8 mg of 5,6,7,8,4'-pentamethoxyflavone and 50.6 mg of 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone with their purities over 97.0% were obtained from 4.0 g of the crude extract of Pericarpium Citri Reticulatae in one-step elution under the conditions of a flow rate of 1.70 mL/min, 800 r/min and the detection wavelength of 280 nm. The obtained fractions were analyzed by high performance liquid chromatography (HPLC), and identified by mass spectrometry (MS), 1H-nuclear magnetic resonance (NMR) and 13C-NMR. The results indicate that HSCCC is a powerful technique for the purification of flavones from Citrus reticulata Blanco.
Sun
Yinshi
Y
College of Agronomy, Shandong Agricultural University, Taian 271018, China.
Liu
Zhengbo
Z
Wang
Jianhu
J
Wang
Ying
Y
Zhu
Lixiang
L
Li
Lailing
L
D002957:Citrus / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D047309:Flavones / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D047309:Flavones