[Simultaneous determination of nitrofurantoin and furazolidone in cosmetics using high performance liquid chromatography].
A method was developed for the simultaneous determination of nitrofurantoin and furazolidone in cosmetics using reversed-phase high performance liquid chromatography. Cosmetics samples of various types, including lotion, cream, emollient, waterpowder and lipstick, were extracted under ultrasonication with acetonitrile-methanol (1:1, v/v). A Kromasil C18 analytical column (250 mm x 4.6 mm, 5 microm) was used for the separation at 25 degrees C. Acetonitrile-0.4% acetic acid (30:70, v/v) was used as the mobile phase at a flow rate of 1.0 mL/min. The detection was performed at 365 nm by a diode array detector. The quantitative analysis was performed using external standard calibration curve. The linear ranges for nitrofurantoin and furazolidone were 0.1-20 mg/L (r = 0.9999). The average recoveries were 88.0%-104.6% with the relative standard deviations of 0.5%-4.8%. The method is convenient, rapid, reliable and suitable for the determination of nitrofurantoin and furazolidone in cosmetics.
Zhang
Qing
Q
Institute of Industrial Product Inspection, Chinese Academy of Inspection and Quarantine, Beijing 100123, China. njuzhangqing@yahoo.com.cn
Wang
Chao
C
Wang
Xing
X
Wu
Ting
T
Ma
Qiang
Q