title: Clonally expanded T lymphocytes from atomic bomb survivors in vitro show no evidence of cytogenetic instability.
abstract: Abstract Genomic instability has been suggested as a mechanism by which exposure to ionizing radiation can lead to cancer in exposed humans. However, the data from human cells needed to support or refute this idea are limited. In our previous study on clonal lymphocyte populations carrying stable-type aberrations derived from A-bomb survivors, we found no increase in the frequency of sporadic additional aberrations among the clonal cell populations compared with the spontaneous frequency in vivo. That work has been extended by using multicolor FISH (mFISH) to quantify the various kinds of chromosome aberrations known to be indicative of genomic instability in cloned T lymphocytes after they were expanded in culture for 25 population doublings. The blood T cells used were obtained from each of two high-dose-exposed survivors (>1 Gy) and two control subjects, and a total of 66 clonal populations (36 from exposed and 30 from control individuals) were established. For each clone, 100 metaphases were examined. In the case of exposed lymphocytes, a total of 39 additional de novo stable, exchange-type aberrations [translocation (t) + derivative chromosome (der)] were found among 3600 cells (1.1%); the corresponding value in the control group was 0.6% (17/3000). Although the ratio (39/3600) obtained from the exposed cases was greater than that of the controls (17/3000), the difference was not statistically significant (P = 0.101). A similar lack of statistical difference was found for the total of all structural chromosome alterations including t, der, dicentrics, duplications, deletions and fragments (P = 0.142). Thus there was no clear evidence suggesting the presence of chromosome instabilities among the clonally expanded lymphocytes in vitro from A-bomb survivors.
authors.affiliation: Departments of Radiobiology and Molecular Epidemiology, Radiation Effects Research Foundation, Minami-ku, Hiroshima, 732-0815, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hamasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Kusunoki
authors.forename: E
authors.initials: E
authors.lastname: Nakashima
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Nakachi
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Kodama
mesh_terms: D000293:Adolescent; D000368:Aged; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D019976:Cloning, Organism; D005260:Female; D006801:Humans; D007564:Japan; D008875:Middle Aged; D009689:Nuclear Warfare; D017741:Survivors; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D055815:Young Adult