title: Biocompatibility analysis of chemomechanical caries removal material Papacárie on cultured fibroblasts and subcutaneous tissue.
abstract: Chemomechanical caries removal allies an atraumatic technique with antimicrobiotic characteristics, minimizing painful stimuli and maximally preserving healthy dental structures. The purpose of this study was to compare the cytotoxic effects of papain-based gel (Papacarie) and another caries-removing substance, Carisolv, to a nontreatment control on cultured fibroblasts in vitro and the biocompatibility in subcutaneous tissue in vivo. The cytotoxicity analysis was performed on fibroblast cultures (NIH-3T3) after 0-, 4-, 8-, and 12-hour exposure (cell viability assay) and after 1-, 3-, 5-, and 7-day exposure (survival assay). In the in vivo study, the 2 compounds were introduced into polyethylene tubes that were implanted into subcutaneous tissues of rats. After 1, 7, 14, 30, and 60 days, tissue samples were examined histologically. Cell viability did not differ between the 2 experimental groups. The control group, however, showed significantly higher percentage viability. There were no differences in cell survival between the control and experimental groups. The histological analysis revealed a moderate inflammatory response at 2 and 7 days and a mild response at 15 days, becoming almost imperceptible by 30 and 60 days in both experimental groups. The 2 tested substances exhibited acceptable biocompatibilities and demonstrated similar responses in the in vitro cytotoxicity and in vivo implantation assay.
authors.affiliation: Nove de Julho University, São Paulo, SP, Brazil. manomartins@gmail.com
authors.forename: Manoela Domingues
authors.initials: MD
authors.lastname: Martins
authors.forename: Kristianne Porta Santos
authors.initials: KP
authors.lastname: Fernandes
authors.forename: Lara Jansinski
authors.initials: LJ
authors.lastname: Motta
authors.forename: Elaine Marcilio
authors.initials: EM
authors.lastname: Santos
authors.forename: Vanessa Christina Santos
authors.initials: VC
authors.lastname: Pavesi
authors.forename: Sandra Kalil
authors.initials: SK
authors.lastname: Bussadori
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003731:Dental Caries / Q000628:therapy*; D003737:Dental Cavity Preparation / Q000295:instrumentation / Q000379:methods*; D003782:Dental Pulp / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D018698:Glutamic Acid / Q000633:toxicity*; D007930:Leucine / Q000633:toxicity*; D008239:Lysine / Q000633:toxicity*; D010206:Papain / Q000633:toxicity*; D051381:Rats
chemical_list: D001672:Biocompatible Materials; C119934:Carisolv; C509557:Papacarie; D018698:Glutamic Acid; D010206:Papain; D007930:Leucine; D008239:Lysine