Proteomics of Arabidopsis redox proteins in response to methyl jasmonate.
Protein redox regulation is increasingly recognized as an important switch of protein activity in yeast, bacteria, mammals and plants. In this study, we identified proteins with potential thiol switches involved in jasmonate signaling, which is essential for plant defense. Methyl jasmonate (MeJA) treatment led to enhanced production of hydrogen peroxide in Arabidopsis leaves and roots, indicating in vivo oxidative stress. With monobromobimane (mBBr) labeling to capture oxidized sulfhydryl groups and 2D gel separation, a total of 35 protein spots that displayed significant redox and/or total protein expression changes were isolated. Using LC-MS/MS, the proteins in 33 spots were identified in both control and MeJA-treated samples. By comparative analysis of mBBr and SyproRuby gel images, we were able to determine many proteins that were redox responsive and proteins that displayed abundance changes in response to MeJA. Interestingly, stress and defense proteins constitute a large group that responded to MeJA. In addition, many cysteine residues involved in the disulfide dynamics were mapped based on tandem MS data. Identification of redox proteins and their cysteine residues involved in the redox regulation allows for a deeper understanding of the jasmonate signaling networks.
Alvarez
Sophie
S
Department of Botany and Zoology, Genetics Institute, The Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA.
Zhu
Mengmeng
M
Chen
Sixue
S
D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000378:metabolism; D002853:Chromatography, Liquid; D003517:Cyclopentanes / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects*; D040901:Proteomics / Q000379:methods; D013438:Sulfhydryl Compounds / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D020543:Proteome; D013438:Sulfhydryl Compounds; C072239:methyl jasmonate; D006861:Hydrogen Peroxide