title: Mycobacterium tuberculosis secreted antigen (MTSA-10) inhibits macrophage response to lipopolysaccharide by redox regulation of phosphatases.
abstract: The present study was undertaken to investigate the possible role of a 10-kDa, secretory antigenic protein of Mtb (MTSA-10) in regulating macrophase response to lipopolysacchride (LPS). MTSA-10 inhibited the lipopolysaccharide (LPS)-induced oxidant species generation in the macrophage. Treatment of macrophages with MTSA-10 activated their protein tyrosine phosphatases (PTPs) in a redox-regulated fashion. These activated phosphatases then interfered with the early events of LPS signaling and lower the strength and magnitude of the signal generated, thereby preventing macrophages from making an effective immune response. Mycobacterium tuberculosis Region of Deletion-1 (RD-1)-specific secretory antigen MTSA-10 (encoded by ORF Rv3874 of Mtb genome) modulated the macrophage signaling machinery and prevented it from responding to further activation by LPS.
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, ICGEB Campus, Aruna Asaf Ali Marg, New Delhi 110 067, India.
authors.forename: Sandip Kumar
authors.initials: SK
authors.lastname: Basu
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Kumar
authors.forename: Niladri
authors.initials: N
authors.lastname: Ganguly
authors.forename: Kanury Venkata Subba
authors.initials: KV
authors.lastname: Rao
authors.forename: Pawan
authors.initials: P
authors.lastname: Sharma
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000276:immunology; D010084:Oxidation-Reduction; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D010744:Phosphoric Monoester Hydrolases