NMR assignment of the domain 513-651 from the SARS-CoV nonstructural protein nsp3.
Sequence-specific NMR assignments of an internal domain of the protein nsp3, nsp3(513-651), which is a part of the SARS coronavirus (SARS-CoV) replicase polyprotein, have been determined, using triple-resonance NMR experiments with the uniformly [(13)C,(15)N]-labeled protein. The complete assignments (>99%) provide the basis for the ongoing three-dimensional structure determination.
Chatterjee
Amarnath
A
Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Johnson
Margaret A
MA
Serrano
Pedro
P
Pedrini
Bill
B
Wüthrich
Kurt
K
D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008970:Molecular Weight; D017434:Protein Structure, Tertiary; D011522:Protons; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
D011522:Protons; D017361:Viral Nonstructural Proteins; C510959:Nsp3 protein, SARS-CoV; D012324:RNA-Dependent RNA Polymerase