Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl PHAs) from cosmetic co-products by Pseudomonas raguenesii sp. nov., isolated from Tetiaroa, French Polynesia.
A new bacterium, designated as strain TE9 was isolated from a microbial mat in French Polynesia and was studied for its ability to synthesize medium chain length poly-beta-hydroxyalkanoates (mcl PHAs) during cultivation on cosmetics co-products. The composition of PHAs was analysed by coupled gas chromatography mass spectroscopy (GC/MS), nuclear magnetic resonance (NMR) and Fourier Transform InfraRed (FTIR) spectroscopy. PHAs were composed of C6-C14 3-hydroxyacids monomers, with a predominance of 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD) and 3-hydroxydodecanoate (3HDD). Differential scanning calorimetry (DSC) experiments allowed the characterization of elastomeric materials with a melting point T(m) near 50 degrees C, enthalpy of fusion DeltaH(m) from 27 to 32 J/g, and glass transition temperature T(g) of -43 degrees C. Molecular weights ranged from 175,000 to 358,000 g/mol. On the basis of the phenotypical features and genotypic investigations, strain TE9 was assigned to the Pseudomonas genus and the name of Pseudomonas raguenesii sp. nov. is proposed.
Simon-Colin
C
C
Institut Français de Recherche pour l'Exploitation de la Mer, Centre de Brest, BIOMAR/BMM, B.P. 70, 29280 Plouzané, France. christelle.simon.colin@ifremer.fr
Alain
K
K
Raguénès
G
G
Schmitt
S
S
Kervarec
N
N
Gouin
C
C
Crassous
P
P
Costa
B
B
Guezennec
J G
JG
D001709:Biotechnology / Q000379:methods*; D002152:Calorimetry, Differential Scanning / Q000379:methods; D003358:Cosmetics / Q000378:metabolism; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005898:Glass; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D011114:Polynesia; D011549:Pseudomonas / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013816:Thermodynamics
D003358:Cosmetics; D003470:Culture Media; D008055:Lipids; D054813:Polyhydroxyalkanoates