Optical and structural properties of copper-oxytocin dications in the gas phase.
We present a joint experimental and theoretical investigation of the structural and optical properties of copper-oxytocin dications in the gas phase. Ion mobility and UV photodissociation experiments were performed, allowing the investigation of the influence of the Cu(2+) ion on the structural and optical properties of oxytocin. Density functional theory calculations were performed to find low energy structures for the bare and complexed peptide and to characterize optical spectral features. Copper complexation induces a drastic change in the structure of the oxytocin peptide. In particular, we predict a 4N chelation of the copper cation which leads to a contraction of the oxytocin ring. The gas phase lowest-energy structures are compared with the X-ray crystal structure of the oxytocin molecule bound to its receptor protein. The optical spectrum of oxytocin complexed with the copper cation displays a global enhancement of the photofragmentation yield as compared to the one recorded for the doubly protonated oxytocin. Moreover, experimental and calculated optical spectra of protonated tyrosine have also been determined, since its leading features are present in oxytocin as well.
Joly
Laure
L
Université de Lyon, F-69622, Lyon, France.
Antoine
Rodolphe
R
Albrieux
Florian
F
Ballivian
Renaud
R
Broyer
Michel
M
Chirot
Fabien
F
Lemoine
Jérôme
J
Dugourd
Philippe
P
Greco
Claudio
C
Mitrić
Roland
R
Bonacić-Koutecký
Vlasta
V
D002413:Cations, Divalent; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005740:Gases; D015394:Molecular Structure; D010121:Oxytocin / Q000737:chemistry*
D002413:Cations, Divalent; D005740:Gases; D010121:Oxytocin; D003300:Copper