DAPI diffusion after intravitreal injection of mesenchymal stem cells in the injured retina of rats.
To evaluate DAPI (4',6-diamidino-2-phenylindole) as a nuclear tracer of stem cell migration and incorporation it was observed the pattern of retinal integration and differentiation of mesenchymal stem cells (MSCs) injected into the vitreous cavity of rat eyes with retinal injury. For this purpose adult rat retinas were submitted to laser damage followed by transplantation of DAPI-labeled BM-MSCs grafts and double-labeled DAPI and quantum dot-labeled BM-MSCs. To assess a possible DAPI diffusion as well as the integration and differentiation of DAPI-labeled BM-MSCs in laser-injured retina, host retinas were evaluated 8 weeks after injury/transplantation. It was demonstrated that, 8 weeks after the transplant, most of the retinal cells in all neural retinal presented nuclear DAPI labeling, specifically in the outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL). Meanwhile, at this point, most of the double-labeled BM-MSCs (DAPI and quantum dot) remained in the vitreous cavity and no retinal cells presented the quantum dot marker. Based on these evidences we concluded that DAPI diffused to adjacent retinal cells while the nanocrystals remained labeling only the transplanted BM-MSCs. Therefore, DAPI is not a useful marker for stem cells in vivo tracing experiments because the DAPI released from dying cells in moment of the transplant are taken up by host cells in the tissue.
Castanheira
Paula
P
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Torquetti
Leonardo Torquetti
LT
Magalhãs
Débora Rodrigues Soares
DR
Nehemy
Marcio B
MB
Goes
Alfredo M
AM
D000818:Animals; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D007211:Indoles / Q000737:chemistry*; D045164:Mesenchymal Stem Cell Transplantation; D045663:Quantum Dots; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000293:injuries* / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000473:pathology
D007211:Indoles; C007293:DAPI