Surgically-induced bile duct injury is followed by early hepatic oxidative stress. A preliminary experimental study in rats.
OBJECTIVE
To study in the rat whether oxidative stress appears early in a model of short-term cholestasis induced by bile duct ligation.


METHODS
Adult male rats underwent proximal bile duct ligation; controls underwent a sham operation. Animals were sacrificed at days 0, 1, 3, 7 and 10. Livers were removed for histological studies and biochemical analysis.


RESULTS
GSH and PSH levels declined earlier in mitochondria than cytosol. Data were analyzed for statistical significance by ANOVA analysis.


CONCLUSIONS
This study represents an experimental model which helps in the interpretation of the damage induced by bile duct injury in humans emphasizing an early detection of bile duct injury after surgery to ensure appropriate treatment and optimal patient outcome.
Lissidini
Germana
G
Section of General Surgery A. De Blasi Department for the Application in Surgery of Innovative Technologies, University of Bari, Bari, Italy.
Piccinni
Giuseppe
G
Portincasa
Piero
P
Grattagliano
Ignazio
I
Gurrado
Angela
A
Testini
Mario
M
D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D003135:Common Bile Duct; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism
D024101:Mitochondrial Proteins; D013438:Sulfhydryl Compounds; D005978:Glutathione