title: A new enzyme-catalytic resonance scattering assay for glucose in serum using cationic surfactant.
abstract: In acetic acid buffer solution, glucose oxidase (GOD) catalyzed the dissolved oxygen oxidation of glucose to form H(2)O(2). In succession, horseradish peroxidase (HRP) catalyzed the H(2)O(2) oxidizing excess I(-) to form I(3)(-). The I(3)(-) combined with a cationic surfactant (CS) such as tetradecyl dimethyl benzyl ammonium chloride (TDMBA) to produce TDMBA-I(3) association particles that exhibited the strongest resonance scattering (RS) peak at 460 nm. The enhanced RS intensity at 460 nm was linear with glucose concentration in the range of 2.0 x 10(-8)-2.0 x 10(-6) mol/L, with a detection limit of 8.5 x 10(-9) mol/L. The glucose in serum samples were assayed by the enzyme catalytic RS assay and by spectrophotometry. The results of both assays showed a close correlation. This assay has simplicity, sensitivity and good specificity for quantitative determination of glucose.
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wei
authors.forename: Ji
authors.initials: J
authors.lastname: Ma
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D055162:Biocatalysis; D001774:Blood Chemical Analysis / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011193:Potassium Iodide / Q000378:metabolism; D012542:Scattering, Radiation; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D013501:Surface-Active Agents; D011193:Potassium Iodide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase