Determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma by high-performance liquid chromatography.
A simple and reliable analytical method based on high-performance liquid chromatography (HPLC) coupled with a diode array detector (DAD) was developed for the determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma using rhoiptelol as an internal standard. Chromatographic separation was carried out on a Sinochrom ODS-AP C(18) column (250 x 4.6 microm i.d., 5 mm) with acetonitrile-10 mM postassium dihydrogen phosphate (pH = 3; 55:45, v/v) as mobile phase, and the detection wavelength was set at 214 nm. The plasma samples were prepared using methanol as protein precipitator. The extraction recovery of Juglanin B ranged from 70.26 to 78.59%, and the calibration curve had a good linearity in the range 0.08-50 microg/mL (r(2) = 0.9932). The RSDs of intra- and inter-day precision ranged from 1.19 to 4.92% and 4.35 to 4.54%, respectively. The HPLC-DAD method described is a simple, rapid and reliable method for the determination of Juglanin B level and for use in studies involving pharmacokinetics.
Huang
Xin-Yi
XY
Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, China.
Duan
Qiu-Yan
QY
Liu
Jun-Xi
JX
Di
Duo-Long
DL
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D036381:Diarylheptanoids / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D029001:Herbal Medicine; D031324:Juglans / Q000737:chemistry*; D016014:Linear Models; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D036381:Diarylheptanoids; D010936:Plant Extracts