[Chemical constituents of Alisma orientalis and their immunosuppressive function].
OBJECTIVE
To investigate the chemical constituents with immunosuppressive function from Alisma orientalis.


METHODS
The chemical constituents were isolated and purified by kinds of column chromatography and its structures were elucidated by NMR spectra and physicochemical properties. Its immunocompentence of lymphocytes taken from spleen of mouse were examined by MTT assay.


RESULTS
Twelve compounds were isolated and identified as clovandiol (1), orientalol E (2), alismoxide (3), alismol (4), 4alpha, l0alpha-dihydroxy-5beta-H-guaj-6-en (5), alismorientols A (6), alisol F (7), alisol A (8), 13beta,17beta-epoxy alisol A (9), alisol B 23-acetate (10), 1H-indole-3-carboxylic acid (11) and cuccinic acid (12). Compounds 9, 10 and alisol A 24-acetate showed immunosuppressive function.


CONCLUSIONS
Compounds 1, 5, 11 and 12 were isolated firstly from this genus,and the NMR spectra data of 1 were corrected firstly, some protostan-type triterpenoids may be developed as new drug with immunosuppressive function.
Zhang
Chaofeng
C
Key Laboratory of Modern Chinese Medicines, Ministry of Education, China Pharmaceutical University, Nanjing 210038, China. njchaofeng@126.com
Zhou
Aichun
A
Zhang
Mian
M
D034902:Alisma / Q000737:chemistry*; D000818:Animals; D002478:Cells, Cultured; D002783:Cholestenones / Q000737:chemistry / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000494:pharmacology; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology
D002783:Cholestenones; D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents; D007211:Indoles; D012717:Sesquiterpenes; D014315:Triterpenes; C457233:alisol A 24-acetate; C526957:alisol B 23-acetate; C516685:alisol F; C000050:alisol A; C012382:indole-3-carboxylic acid; C053720:alismol