title: [The inhibitory effects of siRNA expression vector on cell proliferation and expression of hnRNPB1 gene in lung cancer A549 cells].
abstract: OBJECTIVE
  To investigate the inhibitory effects of specific siRNA on the expression of heterogeneous nuclear ribonucleoproteins (hnRNPB1) and cell proliferation in lung cancer A549 cells.
  
  
  METHODS
  After the construction of siRNA expression vector for hnRNPB1, cultured A549 cells were tansfected with the specific siRNA vector, so RNA of A549 cells was interfered by RNA interference technique. The cells were retrieved at 1st, 4th and 6th week after transfection, cell cycles and cell apoptosis were measured with flow cytometry, the mRNAs levels of hnRNPB1, were detected by fluorescence quantity RT-PCR, the protein levels of hnRNPB1 was detected by Western blot technique.
  
  
  RESULTS
  It was found that plasmid-derived siRNAs could inhibit hnRNPB1 mRNA and protein expression. The expressions of hnRNPB1 mRNA and protein were decreased about 46%-73%, and 77.69%-83.04% respectively. We also observed that siRNA in A549 cell suppressed the cell proliferation by increasing the amounts of G1 cells, and induced cell apoptosis.
  
  
  CONCLUSIONS
  These results demonstrated the effectiveness of siRNA in lung caner cell, which indicated a possible clinical therapy method for lung cancer.
authors.affiliation: Department of Respiratory Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Pu
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Li
authors.forename: Tao-you
authors.initials: TY
authors.lastname: Zhou
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Feng-ming
authors.initials: FM
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: Han
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering