Chromosomal rearrangements during human epidermal keratinocyte differentiation.
Undifferentiated human epidermal keratinocytes are self-renewing stem cells that can be induced to undergo a program of differentiation by varying the calcium chloride concentration in the culture media. We utilize this model of cell differentiation and a 3D chromosome painting technique to document significant changes in the radial arrangement, morphology, and interchromosomal associations between the gene poor chromosome 18 and the gene rich chromosome 19 territories at discrete stages during keratinocyte differentiation. We suggest that changes observed in chromosomal territorial organization provides an architectural basis for genomic function during cell differentiation and provide further support for a chromosome territory code that contributes to gene expression at the global level.
Marella
Narasimharao V
NV
Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.
Seifert
Brandon
B
Nagarajan
Priyadharsini
P
Sinha
Satrajit
S
Berezney
Ronald
R
D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D045584:Chromosome Positioning; D002877:Chromosomes, Human / Q000378:metabolism*; D002887:Chromosomes, Human, Pair 18 / Q000378:metabolism; D002888:Chromosomes, Human, Pair 19 / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism*; D053141:Organelle Size
D015415:Biomarkers