Male-specific expression of Aldh1a1 in mouse and chicken fetal testes: implications for retinoid balance in gonad development.
Balanced production and degradation of retinoids is important in regulating development of several organ systems in the vertebrate embryo. Among these, it is known that retinoic acid (RA), and the retinoid-catabolyzing enzyme CYP26B1 together regulate the sex-specific behavior of germ cells in developing mouse gonads. We report here that the gene encoding a cytosolic class-1 aldehyde dehydrogenase, ALDH1A1, a weak catalyst of RA production, is strongly expressed in a male-specific manner in somatic cells of the developing mouse testis, beginning shortly after Sry expression is first detectable. This expression pattern is conserved in the developing male gonad of the chicken and is dependent on the testis-specific transcription factor SOX9. Our data suggest that low levels of RA may be required for early developmental events in the testis, or that Aldh1a1 expression in the fetus may prefigure a later requirement for ALDH1A1 in regulating spermatogenesis postnatally.
Bowles
Josephine
J
Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Feng
Chun-Wei
CW
Knight
Deon
D
Smith
Craig A
CA
Roeszler
Kelly N
KN
Bagheri-Fam
Stefan
S
Harley
Vincent R
VR
Sinclair
Andrew H
AH
Koopman
Peter
P
D000444:Aldehyde Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D017931:DNA Primers / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D024282:Genes, sry; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011247:Pregnancy; D050697:Retinal Dehydrogenase; D012176:Retinoids / Q000378:metabolism*; D055758:SOX9 Transcription Factor / Q000378:metabolism; D012733:Sex Differentiation; D013737:Testis / Q000196:embryology* / Q000201:enzymology* / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism
D017931:DNA Primers; D007527:Isoenzymes; D012176:Retinoids; D055758:SOX9 Transcription Factor; D014212:Tretinoin; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C515028:ALDH1A1 protein, mouse; D050697:Retinal Dehydrogenase