title: Potential mechanism of action of human growth hormone on isolated human penile erectile tissue.
abstract: OBJECTIVE
  To evaluate the mechanisms of growth hormone (GH) action on isolated human penile erectile tissue. Human GH (hGH) has been suggested to play a role in male reproductive function, including penile erection. Nevertheless, it still remains unclear which intracellular pathways mediate the physiological effects of GH on the human corpus cavernosum (HCC).
  
  
  METHODS
  Using the organ bath technique, the effects of GH were investigated on electrical field stimulation (EFS)-induced relaxation of isolated HCC in the absence and presence of the guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and nitric oxide synthase (NOS) inhibitor N(G)-nitro-l-arginine (l-NOARG, 10 microm). Effects of GH on the production of tissue cyclic guanosine monophosphate (cGMP) in the absence and presence of ODQ and l-NOARG were also elucidated using radioimmunoassay.
  
  
  RESULTS
  ODQ and l-NOARG abolished the relaxation of the tissue induced by EFS, whereas amplitudes were increased by physiological concentrations of GH (1-100 nm). The attenuation of EFS-induced amplitudes by l-NOARG but not ODQ was, in part, reversed by GH. The production of cGMP (pmol cGMP/mg protein) induced by 10 nm GH was abolished in the presence of 10 microm ODQ. In contrast, the combination of GH (10 nm) and l-NOARG (10 microm) maintained cGMP production significantly greater than baseline (0.68 +/- 0.36 vs 1.07 +/- 0.48 pmol cGMP/mg protein).
  
  
  CONCLUSIONS
  Our data provide evidence that GH may act on human HCC by an NO-independent effect on guanylyl cyclase activity and may thus explain how growth factors, such as hGH, regulate male erectile function.
authors.affiliation: Department of Urology and Uro-Oncology, Hannover Medical School, Hannover, Germany. sue_de_99@yahoo.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Uckert
authors.forename: Friedemann
authors.initials: F
authors.lastname: Scheller
authors.forename: Christian G
authors.initials: CG
authors.lastname: Stief
authors.forename: Wolfram H
authors.initials: WH
authors.lastname: Knapp
authors.forename: Michael
authors.initials: M
authors.lastname: Sohn
authors.forename: Armin J
authors.initials: AJ
authors.lastname: Becker
authors.forename: Markus A
authors.initials: MA
authors.lastname: Kuczyk
mesh_terms: D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010413:Penis / Q000187:drug effects*
chemical_list: D019382:Human Growth Hormone