In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines.
In this study, we present in vitro cytotoxicity of iron oxide (Fe(3)O(4)) and manganese oxide (MnO) using live/dead cell assay, lactate dehydrogenase assay, and reactive oxygen species detection with variation of the concentration of nanoparticles (5-500 microg/ml), incubation time (18-96 h), and different human cell lines (lung adenocarcinoma, breast cancer cells, and glioblastoma cells). The surface of nanoparticles is modified with polyethyleneglycol-derivatized phospholipid to enhance the biocompatibility, water-solubility, and stability under an aqueous media. While the cytotoxic effect was negligible for 18 h incubation even at highest concentration of 500 microg/ml, MnO nanoparticle represented higher level of toxicity than those of Fe(3)O(4) and the commercial medical contrast reagent, Feridex after 2 and 4 day incubation time. However, the cytotoxicity of Fe(3)O(4) is equivalent or better than Feridex based on the live/dead cell viability assay. The engineered MnO and Fe(3)O(4) exhibited excellent stability compared with Feridex for a prolonged incubation time.
Choi
Jong Young
JY
Department of Chemical and Biomolecular Engineering (BK21 Program), Institute for the BioCentury, KAIST, Daejeon 305-701, Korea.
Lee
Su Hee
SH
Na
Hyon Bin
HB
An
Kwangjin
K
Hyeon
Taeghwan
T
Seo
Tae Seok
TS
D001681:Biological Assay / Q000379:methods*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000009:adverse effects*; D006801:Humans; D017895:Manganese Compounds / Q000009:adverse effects*; D008422:Materials Testing / Q000379:methods*; D053758:Nanoparticles / Q000009:adverse effects*; D010087:Oxides / Q000009:adverse effects*; D013997:Time Factors
D005290:Ferric Compounds; D017895:Manganese Compounds; D010087:Oxides; C000499:ferric oxide; C027424:manganese oxide