title: Peripheral CD4+CD8+cells are the activated T cells expressed granzyme B (GrB), Foxp3, interleukin 17 (IL-17), at higher levels in Th1/Th2 cytokines.
abstract: Peripheral CD4+CD8+ T cells have been identified as a T cell subset existing in animals and humans. However, the characterization of CD4+CD8+ T cells, their relationship with T memory (T(M)), T effector (T(E)), Th1/Th2, Treg and Th-17, remain unclear. This study was to characterize the CD4+CD8+ T cells. The results from human subjects showed that activated T cells were CD4+CD8+ T cells, comprised CD4(hi)CD8(lo), CD4(hi)CD8(hi) and CD4(lo)CD8(hi) subsets. They expressed CD62L(hi/lo), granzyme B (GrB), CD25, Foxp3, interleukin 17 (IL-17) and the cytokines of both Th1 and Th2, and had cytolytic function. These findings suggested that CD4+CD8+ T cells had over-lap function while they kept diversity, and that T cells could be divided into two major populations: activated and inactivated. Hence, the hypotheses of Th1/Th2, Treg and Th-17 might reflect the positive/negative feedback regulation of immune system. When compared to GrB+CD62L(lo) T effector (T(E)) cells, GrB+CD62L(hi) T central memory effector (T(CME)) cells had a quicker response to virus without CD62L loss.
authors.affiliation: Department of Pediatrics, Columbia University, 3959 Broadway, New York, NY 10032, USA. dx2118@columbia.edu
authors.forename: Dongxu
authors.initials: D
authors.lastname: Xie
authors.forename: Bai
authors.initials: B
authors.lastname: Hai
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Xie
authors.forename: Lihua
authors.initials: L
authors.lastname: Liu
authors.forename: Janet
authors.initials: J
authors.lastname: Ayello
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ma
authors.forename: Junying
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000276:immunology; D053804:Granzymes / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D019041:L-Selectin / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D055815:Young Adult
chemical_list: D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D020381:Interleukin-17; D053645:Interleukin-2 Receptor alpha Subunit; D019041:L-Selectin; D053804:Granzymes