title: Salivary aldehyde dehydrogenase: activity towards aromatic aldehydes and comparison with recombinant ALDH3A1.
abstract: A series of aromatic aldehydes was examined as substrates for salivary aldehyde dehydrogenase (sALDH) and the recombinant ALDH3A1. Para-substituted benzaldehydes, cinnamic aldehyde and 2-naphthaldehydes were found to be excellent substrates, and kinetic parameters for both salivary and recombinant ALDH were nearly identical. It was demonstrated that for the fluorogenic naphthaldehydes the only produced reaction product after incubation in saliva is the carboxylate.
authors.affiliation: Department of Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha Street, PL-02-097, Warsaw, Poland. joanna.giebultowicz@gmail.com
authors.forename: Joanna
authors.initials: J
authors.lastname: Giebułtowicz
authors.forename: Renata
authors.initials: R
authors.lastname: Wolinowska
authors.forename: Anna
authors.initials: A
authors.lastname: Sztybor
authors.forename: Monika
authors.initials: M
authors.lastname: Pietrzak
authors.forename: Piotr
authors.initials: P
authors.lastname: Wroczyński
authors.forename: Jacek
authors.initials: J
authors.lastname: Wierzchowski
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D000447:Aldehydes / Q000737:chemistry*; D001547:Benzaldehydes / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D009281:Naphthalenes / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012463:Saliva / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D000447:Aldehydes; D001547:Benzaldehydes; D009281:Naphthalenes; D011994:Recombinant Proteins; C018686:2-naphthaldehyde; C480021:ALDH3A1 protein, human; D000444:Aldehyde Dehydrogenase