Quinone-mediated decolorization of sulfonated azo dyes by cells and cell extracts from Sphingomonas xenophaga.
The effects of various quinone compounds on the decolorization rates of sulfonated azo dyes by Sphingomonas xenophaga QYY were investigated. The results showed that anthraquinone-2-sulfonate (AQS) was the most effective redox mediator and AQS reduction was the rate-limited step of AQS-mediated decolorization of sulfonated azo dyes. Based on AQS biological toxicity tests, it was assumed that AQS might enter the cells and kill them. In the cytoplasmic extracts from strain QYY, AQS more effectively increased decolorization rates of sulfonated azo dyes than other quinone compounds. In addition, we found a NADH/FMN-dependent AQS reductase using nondenaturing polyacrylamide gel electrophoresis (Native-PAGE).
Jiao
Ling
L
School of Environmental and Biological Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, Dalian University of Technology, Dalian 116024, China. jiaolingjian@yahoo.com.cn
Lu
Hong
H
Zhou
Jiti
J
Wang
Jing
J
D001391:Azo Compounds / Q000378:metabolism*; D016227:Benzoquinones / Q000378:metabolism*; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D020578:Sphingomonas / Q000201:enzymology / Q000378:metabolism*; D013450:Sulfones / Q000378:metabolism*
D001391:Azo Compounds; D016227:Benzoquinones; D004396:Coloring Agents; D013450:Sulfones; C004532:quinone; D016660:NAD(P)H Dehydrogenase (Quinone)