title: Upregulation of GRP78 in renal cell carcinoma and its significance.
abstract: OBJECTIVE
  To investigate the expression of GRP78 in human renal cell carcinoma (RCC) and its significance.
  
  
  METHODS
  We studied RNA and tissue section of a tumor and adjacent nontumorous renal tissues obtained from radical nephrectomy specimens of 42 patients and RCC cell lines. We used reverse transcriptase-PCR and immunohistochemistry to detect the GRP78 mRNA and protein expression, respectively.
  
  
  RESULTS
  Reverse transcriptase-PCR revealed that GRP78 mRNA is positively expressed in RCC cell lines (786-0, OS-RC-2, and Caki-1); the GRP78 mRNA expression in the RCC and adjacent nontumorous renal tissues was 0.88 +/- 0.34 and 0.44 +/- 0.15, respectively (P < .001). The GRP78 protein was found in RCC cell lines. Immunohistochemistry results also showed that the level of GRP78 protein expression of RCC tissues was significantly higher than that of the adjacent nontumorous renal tissues (P < .001). The high levels of GRP78 mRNA and protein expression were related to the large tumor size and high clinical stage (P < .001) but not to sex, age, and cell differentiate (P > .05).
  
  
  CONCLUSIONS
  To our knowledge, the present study is the first to report the upregulated expression of GRP78 and that it is possibly involved in pathogenesis of RCC.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of China. fuwj66@yahoo.com.cn
authors.forename: Weijin
authors.initials: W
authors.lastname: Fu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wu
authors.forename: Jiachu
authors.initials: J
authors.lastname: Li
authors.forename: Zengnan
authors.initials: Z
authors.lastname: Mo
authors.forename: Zhanbing
authors.initials: Z
authors.lastname: Yang
authors.forename: Weihua
authors.initials: W
authors.lastname: Huang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ding
mesh_terms: D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins