Nanocomposite based on depositing platinum nanostructure onto carbon nanotubes through a one-pot, facile synthesis method for amperometric sensing.
Platinum nanoparticles (Pt NPs) were deposited onto multi-walled carbon nanotubes (MWNTs) through direct chemical reduction without any other stabilizing agents. Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry were employed to characterize the morphology of the as-prepared nanocomposite (noted as Pt NPs-MWNTs) and further identify the Pt NPs on the surface of MWNTs. The nanocomposite demonstrated the ability to electrocatalyze the oxidation of hydrogen peroxide and substantially raises the response current. A sensitivity of 591.33 microA mM(-1) cm(-2) was obtained at Pt NPs-MWNTs modified electrode. Thus, we immobilized glucose oxidase (GOD) as a model enzyme on the nanocomposite-based electrode with a thin layer of Nafion to fabricate a glucose biosensor, which showed sensitive and fast response to glucose. The influence of the GOD loading was investigated and the biosensor with an enzyme loading concentration of 10 mg/mL shows optimal performance for glucose detection, that is, a detection limit of 3 microM and a response time of 3s, respectively.
Wen
Dan
D
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, PR China.
Zou
Xiangqin
X
Liu
Ying
Y
Shang
Li
L
Dong
Shaojun
S
D055664:Electrochemical Techniques / Q000379:methods*; D008722:Methods; D053761:Nanocomposites / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009930:Organic Chemicals / Q000138:chemical synthesis; D010984:Platinum / Q000737:chemistry*
D037742:Nanotubes, Carbon; D009930:Organic Chemicals; D010984:Platinum