An integrated microfluidic device for large-scale in situ click chemistry screening.
An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.
Wang
Yanju
Y
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 570 Westwood Plaza, building 114, Los Angeles, CA 90095, USA.
Lin
Wei-Yu
WY
Liu
Kan
K
Lin
Rachel J
RJ
Selke
Matthias
M
Kolb
Hartmuth C
HC
Zhang
Nangang
N
Zhao
Xing-Zhong
XZ
Phelps
Michael E
ME
Shen
Clifton K F
CK
Faull
Kym F
KF
Tseng
Hsian-Rong
HR
D000114:Acetylene / Q000737:chemistry; D000818:Animals; D001386:Azides / Q000737:chemistry; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors / Q000378:metabolism; D002257:Carbonic Anhydrase Inhibitors / Q000032:analysis / Q000494:pharmacology; D002417:Cattle; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D018511:Systems Integration
D001386:Azides; D002257:Carbonic Anhydrase Inhibitors; D024402:Carbonic Anhydrase II; D000114:Acetylene