Rapid determination of phenolic compounds and alkaloids of carob flour by improved liquid chromatography tandem mass spectrometry.
An improved chromatographic method was developed using ultra-performance liquid chromatography-tandem mass spectrometry to identify and quantify phenolic compounds and alkaloids, theobromine and caffeine, in carob flour samples. The developed method has been validated in terms of speed, sensitivity, selectivity, peak efficiency, linearity, reproducibility, limits of detection, and limits of quantification. The chromatographic method allows the identification and quantification of 20 phenolic compounds, that is, phenolic acids, flavonoids, and their aglycone and glucoside forms, together with the determination of the alkaloids, caffeine and theobromine, at low concentration levels all in a short analysis time of less than 20 min.
Ortega
Nàdia
N
Food Technology Department, XaRTA-UTPV, Escuela Tecnica Superior de Ingenieria Agraria Universidad de Lleida, Av/Alcalde Rovira Roure 191, Lleida, Spain.
Macià
Alba
A
Romero
Maria-Paz
MP
Trullols
Esther
E
Morello
Jose-Ramón
JR
Anglès
Neus
N
Motilva
Maria-Jose
MJ
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005433:Flour / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000470:Alkaloids; D010636:Phenols