Two-hybrid analysis identifies PSMD11, a non-ATPase subunit of the proteasome, as a novel interaction partner of AMP-activated protein kinase.
Mammalian AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine protein kinase that acts as a sensor of cellular energy status. It interacts with a great variety of different substrates leading to short-term (i.e. regulation of the activity of different enzymes by direct phosphorylation) and long-term effects (i.e. regulation of transcriptional activity of different transcription factors). In this work, we describe the use of the yeast two-hybrid technology to identify additional proteins that interact with the different subunits of AMPK. We have performed three yeast two-hybrid screenings of a human skeletal muscle cDNA library using three different baits: a constitutively active form of AMPKalpha2 (LexA-AMPKalpha2-T172D) co-expressed with AMPKgamma1, LexA-AMPKbeta2 and LexA-AMPKgamma3. Our results identify novel interaction partners of AMPK in human skeletal muscle. We also further characterize the interaction of AMPK with one of these novel interacting proteins, the non-ATPase subunit of the proteasome PSMD11. Our results indicate that AMPK is able to interact physically with this subunit and modify its phosphorylation status, supporting a possible role for AMPK in regulating proteasome function.
Moreno
Daniel
D
Instituto de Biomedicina de Valencia, CSIC and CIBER de Enfermedades Raras (CIBERER), Jaime Roig 11, 46010 Valencia, Spain.
Viana
Rosa
R
Sanz
Pascual
P
D055372:AMP-Activated Protein Kinases / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D046912:Multiprotein Complexes / Q000276:immunology / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D046988:Proteasome Endopeptidase Complex / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
D046912:Multiprotein Complexes; D055372:AMP-Activated Protein Kinases; C549955:PSMD11 protein, human; D046988:Proteasome Endopeptidase Complex