title: The role of ribose on oxidative stress during hypoxic exercise: a pilot study.
abstract: Oxygen free radicals are produced during stress, are unstable, and potentially interact with other cellular components or molecules. This reactivity can influence cellular function, including a prolongation in tissue recovery following exercise. We tested the effect of ribose (d-ribose), a pentose carbohydrate, in a double-blinded, crossover study on markers of free radical production during hypoxic exercise. Seven healthy volunteers cycled at their lactate threshold for 25 minutes while inhaling 16% O(2) with a subsequent 60-minute resting period at room air. Subjects ingested either placebo or 7 g of ribose in 250 mL of water before and after the exercise session. Urinary malondialdehyde (MDA) and plasma reduced glutathione levels increased significantly during placebo ingestion (0.2 +/- 0.03 nM/mg and 0.26 +/- 0.29 microM, respectively) but were lower with ribose supplementation (0.04 +/- 0.03 nM/mg and 0.38 +/- 0.29 microM, respectively; P < .05). Uric acid levels were similar between groups (ribose vs. placebo, 4.55 +/- 0.06 mg/dL vs. 4.67 +/- 0.06 mg/dL). Ribose demonstrated a beneficial trend in lower MDA and reduced glutathione levels during hypoxic stress.
authors.affiliation: Department of Health and Human Development, Montana State University, Bozeman, 59717, USA. john.seifert@montana.edu
authors.forename: John G
authors.initials: JG
authors.lastname: Seifert
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Subudhi
authors.forename: Min-Xin
authors.initials: MX
authors.lastname: Fu
authors.forename: Karen L
authors.initials: KL
authors.lastname: Riska
authors.forename: Jeff C
authors.initials: JC
authors.lastname: John
authors.forename: Linda M
authors.initials: LM
authors.lastname: Shecterle
authors.forename: John A
authors.initials: JA
authors.lastname: St Cyr
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001642:Bicycling / Q000502:physiology*; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000378:metabolism; D019587:Dietary Supplements; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000097:blood*; D006801:Humans; D000860:Hypoxia / Q000188:drug therapy* / Q000503:physiopathology*; D019344:Lactic Acid / Q000378:metabolism; D008315:Malondialdehyde / Q000652:urine*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010865:Pilot Projects; D012266:Ribose / Q000378:metabolism / Q000502:physiology*
chemical_list: D000975:Antioxidants; D004040:Dietary Carbohydrates; D005609:Free Radicals; D019344:Lactic Acid; D008315:Malondialdehyde; D012266:Ribose; D005978:Glutathione; D010100:Oxygen