Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs.
The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.
Islam-Faridi
M N
MN
Southern Institute of Forest Genetics, Southern Research Station, U.S. Forest Service, Forest Tree Molecular Cytogenetics Laboratory, Department of Ecosystem Science and Management, Texas A&M University, College Station, TX, USA. nfaridi@tamu.edu
Nelson
C D
CD
DiFazio
S P
SP
Gunter
L E
LE
Tuskan
G A
GA
D017360:Arabidopsis / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D020732:Cytogenetic Analysis; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D032107:Populus / Q000145:classification / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D016615:Telomere / Q000235:genetics
D018744:DNA, Plant; D004275:DNA, Ribosomal; D005819:Genetic Markers