title: Detection of Cucumber mosaic virus and typing using serological and molecular methods in Razavi Khorasan Province.
abstract: Investigation was conducted during 2006 and 2007 to detect and determine typing of Cucumber mosaic virus (CMV) in field-collected samples from Razavi Khorasan Province. Leaves showing chlorosis, mosaic, distortion and shoestring symptoms were collected from the Mashhad Regions (Astan-e-ghods and Khaje-rabie), Neishabour, Torbat-e-heidarye, Ghochan, Chenaran, Fariman, Shirvan, Kalat and Kashmar. Samples were transferred to laboratory in order to detect the virus in the collected samples, Samples were tested by DAS-ELISA. Typing was done for those serologically positive-reacted samples by RT-PCR-RFLP. Specific primers have amplified 650 bp fragments of RNA2, in RT-PCR assay. Digestion was performed by restriction enzyme MluI. Present research showed that all of collected samples were in subgroup IA.
authors.affiliation: Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Golnaz
authors.forename: B
authors.initials: B
authors.lastname: Jafarpour
authors.forename: M F
authors.initials: MF
authors.lastname: Rastegar
authors.forename: M A
authors.initials: MA
authors.lastname: Sabokkhiz
mesh_terms: D017799:Cucumovirus / Q000235:genetics / Q000302:isolation & purification; D019662:Cucurbitaceae / Q000033:anatomy & histology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D007492:Iran; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D012698:Serologic Tests / Q000379:methods*
chemical_list: D004279:DNA, Viral