title: In vivo fate mapping with SCL regulatory elements identifies progenitors for primitive and definitive hematopoiesis in mice.
abstract: One of the principal issues facing biomedical research is to elucidate developmental pathways and to establish the fate of stem and progenitor cells in vivo. Hematopoiesis, the process of blood cell formation, provides a powerful experimental system for investigating this process. Here, we employ transcriptional regulatory elements from the stem cell leukemia (SCL) gene to selectively label primitive and definitive hematopoiesis. We report that SCL-labelled cells arising in the mid to late streak embryo give rise to primitive red blood cells but fail to contribute to the vascular system of the developing embryo. Restricting SCL-marking to different stages of foetal development, we identify a second population of multilineage progenitors, proficient in contributing to adult erythroid, myeloid and lymphoid cells. The distinct lineage-restricted potential of SCL-labelled early progenitors demonstrates that primitive erythroid cell fate specification is initiated during mid gastrulation. Our data also suggest that the transition from a hemangioblastic precursors with endothelial and blood forming potential to a committed hematopoietic progenitor must have occurred prior to SCL-marking of definitive multilineage blood precursors.
authors.affiliation: Medical Centre of the Johannes Gutenberg-University Mainz, Institute for Toxicology, 55131 Mainz, Germany. bockamp@uni-mainz.de
authors.forename: Ernesto
authors.initials: E
authors.lastname: Bockamp
authors.forename: Cecilia
authors.initials: C
authors.lastname: Antunes
authors.forename: Stefan
authors.initials: S
authors.lastname: Liebner
authors.forename: Steffen
authors.initials: S
authors.lastname: Schmitt
authors.forename: Nina
authors.initials: N
authors.lastname: Cabezas-Wallscheid
authors.forename: Rosario
authors.initials: R
authors.lastname: Heck
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ohnngemach
authors.forename: Barbara
authors.initials: B
authors.lastname: Oesch-Bartlomowicz
authors.forename: Christof
authors.initials: C
authors.lastname: Rickert
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Sanchez
authors.forename: Jan
authors.initials: J
authors.lastname: Hengstler
authors.forename: Bernd
authors.initials: B
authors.lastname: Kaina
authors.forename: Anne
authors.initials: A
authors.lastname: Wilson
authors.forename: Andreas
authors.initials: A
authors.lastname: Trumpp
authors.forename: Leonid
authors.initials: L
authors.lastname: Eshkind
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000502:physiology*; D019070:Cell Lineage; D047108:Embryonic Development; D005434:Flow Cytometry; D055879:Gene Knock-In Techniques; D006410:Hematopoiesis; D051379:Mice; D018613:Microscopy, Confocal; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; C489366:Tal1 protein, mouse