title: Molecular dissection of the prototype foamy virus (PFV) RNA 5'-UTR identifies essential elements of a ribosomal shunt.
abstract: The prototype foamy virus (PFV) is a nonpathogenic retrovirus that shows promise as a vector for gene transfer. The PFV (pre)genomic RNA starts with a long complex leader that can be folded into an elongated hairpin, suggesting an alternative strategy to cap-dependent linear scanning for translation initiation of the downstream GAG open reading frame (ORF). We found that the PFV leader carries several short ORFs (sORFs), with the three 5'-proximal sORFs located upstream of a structural element. Scanning-inhibitory hairpin insertion analysis suggested a ribosomal shunt mechanism, whereby ribosomes start scanning at the leader 5'-end and initiate at the downstream ORF via bypass of the central leader regions, which are inhibitory for scanning. We show that the efficiency of shunting depends strongly on the stability of the structural element located downstream of either sORFs A/A' or sORF B, and on the translation event at the corresponding 5'-proximal sORF. The PFV shunting strategy mirrors that of Cauliflower mosaic virus in plants; however, in mammals shunting can operate in the presence of a less stable structural element, although it is greatly improved by increasing the number of base pairings. At least one shunt configuration was found in primate FV (pre)genomic RNAs.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Schepetilnikov
authors.forename: Gregory
authors.initials: G
authors.lastname: Schott
authors.forename: Konstantina
authors.initials: K
authors.lastname: Katsarou
authors.forename: Odon
authors.initials: O
authors.lastname: Thiébeauld
authors.forename: Mario
authors.initials: M
authors.lastname: Keller
authors.forename: Lyubov A
authors.initials: LA
authors.lastname: Ryabova
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D002460:Cell Line; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics; D016366:Open Reading Frames; D010442:Peptide Chain Initiation, Translational; D012367:RNA, Viral / Q000737:chemistry*; D012270:Ribosomes / Q000378:metabolism; D016092:Spumavirus / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D015683:Gene Products, gag; D012367:RNA, Viral