Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic system.
The Epic system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.
Wu
Meng
M
Department of Neuroscience, High Throughput Biology Center and Johns Hopkins Ion Channel Center, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA.
Long
Shunyou
S
Frutos
Anthony G
AG
Eichelberger
Maryna
M
Li
Min
M
Fang
Ye
Y
D048948:14-3-3 Proteins / Q000378:metabolism*; D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000378:metabolism*; D010766:Phosphorylation
D048948:14-3-3 Proteins; D010455:Peptides; D017868:Cyclic AMP-Dependent Protein Kinases