Functional analysis of GS28, an intra-Golgi SNARE, in Caenorhabditis elegans.
Intra-Golgi retrograde transport is assumed to maintain Golgi function by recycling Golgi-resident proteins to younger cisternae in the progression of entire Golgi stack from cis to trans. GS28 (Golgi SNARE of 28 kDa, also known as GOS28) is a Golgi-localized SNARE protein and has been implicated in intra-Golgi retrograde transport. However, the in vivo functions of GS28, and consequently, the roles of the intra-Golgi retrograde transport in animal development are largely unknown. In this study, we generated deletion mutants of Caenorhabditis elegans GS28 and performed a synthetic lethal RNAi screen using GS28 mutants. We found that another Golgi-localized SNARE, Ykt6, functions cooperatively with GS28 in embryonic development. During post-embryonic development, GS28 mutants exhibited reduced seam cell numbers and a missing ray phenotype under Ykt6 knockdown conditions, suggesting that cell proliferation and/or differentiation of stem cell-like seam cells are impaired in GS28- and Ykt6-depleted worms. We also demonstrated that GS28 and Ykt6 act redundantly for the proper expression of Golgi-resident proteins in adult intestinal cells. This study reveals the in vivo importance of the Golgi-localized SNAREs GS28 and Ykt6.
Maekawa
Masashi
M
Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan.
Inoue
Takao
T
Kobuna
Hiroyuki
H
Nishimura
Taki
T
Gengyo-Ando
Keiko
K
Mitani
Shohei
S
Arai
Hiroyuki
H
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D051379:Mice; D050683:R-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D050600:SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology
D029742:Caenorhabditis elegans Proteins; D050683:R-SNARE Proteins; D050600:SNARE Proteins; C491482:YKT6 protein, human