Age-specific prevalence of HPV genotypes in cervical cytology samples with equivocal or low-grade lesions.
BACKGROUND
To define the spectrum of human papillomavirus (HPV) types and establish an age limit for triage HPV testing in atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesion (LSIL).


METHODS
343 liquid-based cytological samples from the population-based screening programme with minor abnormalities were subjected to HPV genotyping (Linear Array, Roche, Basel, Switzerland).


RESULTS
High-risk human papillomavirus (HR-HPV) was found in 71% of LSIL and 49% of ASCUS cases (P<0.001). High-risk human papillomavirus prevalence was age-dependent in LSIL (P=0.01), with decreasing prevalence until the age of 50 years, followed by a slight increase. Human papillomavirus type 16 was the most common HR-HPV, found in 23% of HPV-positive women. Human papillomavirus type 18 was the sixth most common, found in 9.9% (P<0.001). An age-dependent quadratic trend was observed for multiple infections (P=0.01) with a trough at about 42 years. The most common HR-HPV types to show a coinfection with HPV16 (clade 9) were HPV39 (28%), 45 (38%), and 59 (46%), belonging to HPV18 clade 7. The frequency of low-risk (LR) vs probable HR and HR-HPV also followed an age-dependent quadratic trend.


CONCLUSIONS
After the age of 25 years, HR-HPV prevalence is similar in LSIL and ASCUS cases, motivating a low age limit for triage HPV testing. Multiple infections and LR/HR-HPV dominance are age-dependent. Genotyping in longitudinal design is needed to elucidate the importance of multiple infections in cancer progression and in cross-protection from vaccination.
Brismar-Wendel
S
S
Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynaecology, Karolinska University Hospital Huddinge, Karolinska Institutet, 141 86 Stockholm, Sweden.
Froberg
M
M
Hjerpe
A
A
Andersson
S
S
Johansson
B
B
D000328:Adult; D000367:Age Factors; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014626:Vaginal Smears