Feminization and alteration of Drosophila taste neurons induce reciprocal effects on male avoidance behavior.
Taste perception allows most animals to find edible food, potential mates, and avoid ingesting toxic molecules. Intriguingly, a small group of Drosophila taste neurones (expressing Gr66a-Gal4) involved in the perception of bitter substances is also used to detect 7-tricosene (7-T), a male cuticular pheromone. Male flies tend to be inhibited by 7-T whereas females are stimulated by this pheromone. To better understand their role on male courtship, Gr66a-Gal4 neurons were genetically feminized or altered with various transgenes, and the response of transgenic males was measured toward live targets carrying various amounts of 7-T, or of bitter molecules (caffeine, quinine and berberine). Surprisingly, tester males with feminized taste neurons showed an increased dose-dependent avoidance toward targets with high level of any of these substances, compared to other tester males. Conversely, males with altered neurons showed no, or very little avoidance. Moreover, the surgical ablation of the sensory appendages carrying these taste neurons differently affected the behavioral response of the various tester males. The fact that this manipulation did not affect the courtship toward control females nor the locomotor activity of tester males suggests that Gr66a-Gal4 neurons are involved in the sex-specific perception of molecules inducing male avoidance behavior.
Lacaille
Fabien
F
Unité Mixte de Recherche 5548 Associée au Centre National de la Recherche Scientifique, Faculté des Sciences, Université de Bourgogne, 6, Bd Gabriel, 21 000, Dijon, France.
Everaerts
Claude
C
Ferveur
Jean-François
JF
D000475:Alkenes / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology*; D005260:Female; D005838:Genotype; D008297:Male; D008954:Models, Biological; D008957:Models, Genetic; D009474:Neurons / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism; D012737:Sex Factors; D012726:Sexual Behavior, Animal; D055697:Taste Perception / Q000235:genetics*; D019076:Transgenes
D000475:Alkenes; D010675:Pheromones; C050395:7-tricosene