title: [Effect of calmodulin antagonist O-4-ethoxyl- butyl-berbamine on the proliferation of human breast cancer cell line MCF-7 and its relevant mechanism].
abstract: OBJECTIVE
  To investigate the effect of calmodulin antagonist O-(4-ethoxyl-butyl)-berbamine (EBB) on proliferation of human breast cancer cell MCF-7 and its possible mechanism.
  
  
  METHODS
  MTT assay was used to analyze the effect of EBB on tumor cells growth. Flow cytometry was used to detect its impact on the cell cycle of MCF-7 cells. Immunofluoresce labeling technique and laser scanning confocal microscope were used to reveal the changes of the microtubule, microfilament, mitochondrion, and endoplasmic reticulum in the cells.
  
  
  RESULTS
  The IC50 value of EBB in MCF-7 cells was (13.0 +/- 3.7) micromol/L. MCF-7 cells were arrested at S phase after EBB treatment. Meanwhile, depolymerization of the microtubule and microfilament, impairment of the mitochondrion and swelling of endoplasmic reticulum were observed.
  
  
  CONCLUSIONS
  EBB arrests MCF-7 cells at S phase by inhibiting the growth of MCF-7 cells, which may be related to the changes of structures and functions of the microtubule, microfilament, mitochondrion, and endoplasmic reticulum.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Dai
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Fan
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Xiong
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.forename: Chun-Zheng
authors.initials: CZ
authors.lastname: Yang
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Zhu
mesh_terms: D044182:Benzylisoquinolines / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans
chemical_list: D044182:Benzylisoquinolines; D002147:Calmodulin; C050387:O-(4-ethoxybutyl)berbamine