Virtual terminator nucleotides for next-generation DNA sequencing.
We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
Bowers
Jayson
J
Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.
Mitchell
Judith
J
Beer
Eric
E
Buzby
Philip R
PR
Causey
Marie
M
Efcavitch
J William
JW
Jarosz
Mirna
M
Krzymanska-Olejnik
Edyta
E
Kung
Li
L
Lipson
Doron
D
Lowman
Geoffrey M
GM
Marappan
Subramanian
S
McInerney
Peter
P
Platt
Adam
A
Roy
Atanu
A
Siddiqi
Suhaib M
SM
Steinmann
Kathleen
K
Thompson
John F
JF
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D022202:Chromosomes, Artificial, Bacterial / Q000737:chemistry* / Q000235:genetics; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D004285:Dogs; D009711:Nucleotides / Q000737:chemistry* / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013379:Substrate Specificity
D009711:Nucleotides; D004247:DNA