Matrix metalloproteinase-1 inhibitory activity of Kaempferia pandurata Roxb.
Matrix metalloproteinase (MMP)-1 is a superfamily of zinc-dependent endopeptidases that are capable of degrading all components of the extracellular matrix. Kaempferia pandurata extract (0.01-0.5 microg/mL) significantly reduced the expression of MMP-1 and induced the expression of type 1 procollagen at the protein and mRNA levels in a dose-dependent manner. Ultraviolet (UV)-induced MMP-1 initiates cleavage of fibrillar collagen. Once cleaved by MMP-1, collagen can be further degraded by elevated levels of MMP-3 and MMP-9. It was found that increased MMP-1 expression due to UV irradiation was mediated by activation of mitogen-activated protein kinases such as extracellular-regulated kinase (ERK), Jun N-terminal kinase (JNK), and p38 kinase. Treatment of K. pandurata extract in the range of 0.01-0.5 microg/mL inhibited the UV-induced phosphorylations of ERK, JNK, and p38, respectively. Moreover, inhibition of phosphorylated ERK, JNK, and p38 by K. pandurata extract resulted in decreased c-Fos expression and c-Jun phosphorylation induced by UV light. The results strongly suggest that K. pandurata is potentially useful for the prevention and treatment of skin aging.
Shim
Jae-Seok
JS
Department of Biomaterials Science, Yonsei University, Seoul, Republic of Korea.
Choi
Eun-Jung
EJ
Lee
Chan-Woo
CW
Kim
Han-Sung
HS
Hwang
Jae-Kwan
JK
D000375:Aging / Q000187:drug effects; D002460:Cell Line; D024042:Collagen Type I / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011347:Procollagen / Q000096:biosynthesis*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D027343:Rhizome; D012867:Skin / Q000187:drug effects; D014466:Ultraviolet Rays; D030521:Zingiberaceae
D024042:Collagen Type I; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011347:Procollagen; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases