title: Continuous lipase-catalyzed production of fatty acid ethyl esters from soybean oil in compressed fluids.
abstract: This work investigates the continuous production of fatty acid ethyl esters from soybean oil in compressed fluids, namely carbon dioxide, propane and n-butane, using immobilized Novozym 435 as catalyst. The experiments were performed in a packed-bed bioreactor evaluating the effects of temperature in the range of 30-70 degrees C, from 50 to 150 bar, oil to ethanol molar ratio of 1:6-1:18 and solvent to substrates mass ratio of 4:1-10:1. In contrast to the use of carbon dioxide and n-butane, results showed that lipase-catalyzed alcoholysis in a continuous tubular reactor in compressed propane might be a potential route to biodiesel production as high reaction conversions were achieved at mild temperature (70 degrees C) and pressure (60 bar) conditions in short reaction times.
authors.affiliation: Programa de Pós-Graduação em Engenharia de Alimentos, URI-Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Dalla Rosa
authors.forename: M B
authors.initials: MB
authors.lastname: Morandim
authors.forename: J L
authors.initials: JL
authors.lastname: Ninow
authors.forename: D
authors.initials: D
authors.lastname: Oliveira
authors.forename: H
authors.initials: H
authors.lastname: Treichel
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
mesh_terms: D001674:Bioelectric Energy Sources; D001709:Biotechnology / Q000379:methods; D002073:Butanes / Q000737:chemistry; D002175:Candida / Q000201:enzymology; D002245:Carbon Dioxide / Q000737:chemistry; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D011312:Pressure; D011407:Propane / Q000737:chemistry; D013025:Glycine max / Q000378:metabolism*; D013696:Temperature
chemical_list: D002073:Butanes; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D010938:Plant Oils; D002245:Carbon Dioxide; C046888:butane; C093667:Novozyme 435; D008049:Lipase; D011407:Propane