title: Molecular characterization and expression analysis of Acmago and AcY14 in Antrodia cinnamomea.
abstract: Mago nashi (Mago) and Y14 proteins, highly conserved among eukaryotes, participate in mRNA localization and splicing, and as such play important roles in oogenesis, embryogenesis and germ-line sex determination during animal development. Here we identified mago (Acmago) and Y14 (AcY14) homologues derived from Antrodia cinnamomea. Acmago encodes 149 amino acids and AcY14 encodes 168 amino acids. Multiple amino acid sequence alignment as well as secondary and tertiary structure prediction showed that AcMago and AcY14 have similar protein structure to the reported crystal structures of other Mago and Y14 proteins. During fungal development both Acmago and AcY14 genes were abundantly expressed in natural basidiomes. This is the first report of the molecular characterization and expression analysis of the mago and Y14 genes from fungi.
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan. fhchu@ntu.edu.tw <fhchu@ntu.edu.tw>
authors.forename: Fang-Hua
authors.initials: FH
authors.lastname: Chu
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Chen
authors.forename: Chen-Hsien
authors.initials: CH
authors.lastname: Lee
authors.forename: Tun-Tschu
authors.initials: TT
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D055491:Antrodia / Q000235:genetics*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010802:Phylogeny; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005656:Fungal Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins