A dumbbell-shaped small molecule that promotes cell adhesion and growth.
During an image-based phenotype screening of our chemical library, we noted a small molecule that boosts the adhesion and growth of human cells. Chemical and cell biological experiments suggest that the diaryldispirotripiperazine derivative (adhesamine) targets selective cell-surface glycosaminoglycans, especially heparan sulfate, for increasing cell adhesion and growth. The addition of adhesamine to the culture medium enables the adhesion of even floating lymphocytes to cell culture plates and the microinjection into them. Unlike poly-L-lysine, adhesamine induces apparently normal cell adhesion accompanied by organized actin structures and activation of focal adhesion kinase and ERK1/2 mitogen-activated protein kinases. Adhesamine may be useful as a cell-attaching reagent for cell engineering and basic cell biology.
Yamazoe
Sayumi
S
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
Shimogawa
Hiroki
H
Sato
Shin-ichi
S
Esko
Jeffrey D
JD
Uesugi
Motonari
M
D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D054852:Small Molecule Libraries / Q000494:pharmacology; D014014:Tissue Adhesives / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods
D000199:Actins; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D048052:Mitogen-Activated Protein Kinase 3; D054852:Small Molecule Libraries; D014014:Tissue Adhesives