Effect of excessive acute-phase response on liver regeneration after partial hepatectomy in rats.
OBJECTIVE
Acute-phase response (APR) is known to have a protective role against infection and tissue injury including partial hepatectomy (HTX). In this study, the effect of excessive APR on liver regeneration was investigated using a rat hepatectomized model.


METHODS
Experimental APR was induced by subcutaneous injection of turpentine oil, immediately, 24 h and 48 h after 50% HTX. Serum IL-6 and hepatic alpha2-macroglobulin mRNA was evaluated in rats receiving turpentine or saline as control after HTX. DNA synthesis and PCNA labeling index in the remnant liver was also investigated. Furthermore, in vitro total hepatic protein synthesis (TPS) and secretory protein synthesis (SPS) in the remnant liver were measured.


RESULTS
Liver regeneration was significantly inhibited after HTX in the turpentine group, compared to the control. DNA synthesis and PCNA labeling index was also significantly decreased on days 1 and 2. A remarkable increase in the serum IL-6 and hepatic alpha2-macroglobulin mRNA was found in the turpentine group. Furthermore, SPS was significantly upregulated in the turpentine group, although TPS was almost similar between the groups.


CONCLUSIONS
Induction of excessive APR after HTX inhibits liver regeneration. Acute phase protein synthesis is remarkably upregulated within the capacity of hepatic protein synthesis, most probably by a reduction in replicative protein synthesis, suggesting that APR may be more appropriate biological response for ensuring immediate survival.
Kusashio
Kimihiko
K
Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Shimizu
Hiroaki
H
Kimura
Fumio
F
Yoshidome
Hiroyuki
H
Ohtsuka
Masayuki
M
Kato
Atsushi
A
Yoshitomi
Hideyuki
H
Furukawa
Katsunori
K
Fukada
Tadaomi
T
Miyazaki
Masaru
M
D000210:Acute-Phase Reaction / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology*; D000818:Animals; D004195:Disease Models, Animal; D006498:Hepatectomy; D015850:Interleukin-6 / Q000097:blood; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D009929:Organ Size; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000378:metabolism
D015850:Interleukin-6; D018809:Proliferating Cell Nuclear Antigen; D000511:alpha-Macroglobulins; D014425:Turpentine