title: Applications of nanopipettes in bionanotechnology.
abstract: At present, technical hurdles remain in probing biochemical processes in living cells and organisms at nanometre spatial resolution, millisecond time resolution and with high specificity and single-molecule sensitivity. Owing to its unique shape, size and electrical properties, the nanopipette has been used to obtain high-resolution topographic images of live cells under physiological conditions, and to create nanoscale features by controlled delivery of biomolecules. In the present paper, I discuss recent progress in the development of a family of new methods for nanosensing and nanomanipulation using nanopipettes.
authors.affiliation: Molecular Medicine, National Heart and Lung Institute, and Chemical Biology Centre, Imperial College London, London SW7 2AZ, UK. l.ying@imperial.ac.uk
authors.forename: Liming
authors.initials: L
authors.lastname: Ying
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D020527:Microscopy, Scanning Probe; D036103:Nanotechnology / Q000379:methods*