Complete backbone assignment of a Ca2+-binding protein of the betagamma-crystallin superfamily from Methanosarcina acetivorans, at two denaturant concentrations.
We report here almost complete backbone assignment of a Ca(2+)-binding protein of the betagamma-crystallin superfamily from Methanosarcina acetivorans, at two denaturant (GdmCl) concentrations, using double and triple resonance experiments. These NMR assignments will be useful to understand the unfolding path of this protein.
Barnwal
Ravi P
RP
Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Coloba, Mumbai, 400005, India. barnwal@u.washington.edu
Agarwal
Geetika
G
Sharma
Yogendra
Y
Chary
Kandala V R
KV
D000595:Amino Acid Sequence; D001665:Binding Sites; D002118:Calcium / Q000737:chemistry*; D002247:Carbon Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D017020:Methanosarcina / Q000737:chemistry*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000737:chemistry; D011485:Protein Binding; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D011522:Protons; D012996:Solutions; D038222:gamma-Crystallins / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D021122:Protein Subunits; D011522:Protons; D012996:Solutions; D038222:gamma-Crystallins; D002118:Calcium