Responses of the hamster chorda tympani nerve to sucrose+acid and sucrose+citrate taste mixtures.
Studies of taste receptor cells, chorda tympani (CT) neurons, and brainstem neurons show stimulus interactions in the form of inhibition or enhancement of the effectiveness of sucrose when mixed with acids or citrate salts, respectively. To investigate further the effects of acids and the trivalent citrate anion on sucrose responses in hamsters (Mesocricetus auratus), we recorded multifiber CT responses to 100 mM sucrose; a concentration series of HCl, citric acid, acetic acid, sodium citrate (with and without amiloride added), potassium citrate, and all binary combinations of acids and salts with 100 mM sucrose. Compared with response additivity, sucrose responses were increasingly suppressed in acid + sucrose mixtures with increases in titratable acidity, but HCl and citric acid were more effective suppressors than acetic acid. Citrate salts suppressed sucrose responses and baseline CT neural activity to a similar degree. Citrate salts also elicited prolonged, concentration-dependent, water-rinse responses. The specific loss in sucrose effectiveness as a CT stimulus with increasing titratable acidity was confirmed; however, no increase in sucrose effectiveness was found with the addition of citrate. Further study is needed to define the chemical basis for effects of acids and salts in taste mixtures.
Formaker
Bradley K
BK
Department of Oral Health and Diagnostic Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA. Brad@neuron.uchc.edu
Lin
Hsung
H
Hettinger
Thomas P
TP
Frank
Marion E
ME
D000143:Acids / Q000378:metabolism*; D000818:Animals; D002814:Chorda Tympani Nerve / Q000502:physiology*; D002951:Citrates / Q000378:metabolism*; D006224:Cricetinae; D004594:Electrophysiology; D008297:Male; D013395:Sucrose / Q000378:metabolism*; D013649:Taste
D000143:Acids; D002951:Citrates; D013395:Sucrose