title: Protective effect of augmenter of liver regeneration on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells.
abstract: BACKGROUND
  Hydrogen peroxide, as other reactive oxygen species (ROS) produced during redox processes, induces lipid membrane peroxidation and protein degeneration causing cell apoptosis. ROS are recently considered as messengers in cell signalling processes, which, through reversible protein disulphide bridges formation, activate regulatory factors of cell proliferation and apoptosis. Disulphide bridges formation is catalysed by sulphydryl oxidase enzymes.
  
  
  OBJECTIVE
  The neuroprotective effect of ALR protein (Alrp), a sulphydryl oxidase enzyme, on H(2)O(2)-induced apoptosis in SH-SY5Y cells has been evaluated.
  
  
  METHODS
  Cell viability, flow cytometric evaluation of apoptotic cells, fluorescent changes of nuclear morphology, immunocytochemistry Alrp detection, Western blot evaluation of mitochondrial cyt c release and mitochondrial swelling were determined.
  
  
  RESULTS
  Alrp prevents the H(2)O(2)-induced cell viability loss, apoptotic cell death and mitochondrial swelling in SH-SY5Y cells in culture.
  
  
  CONCLUSIONS
  The data demonstrate that Alrp improves SH-SY5Y cells survival in H(2)O(2)-induced apoptosis. It is speculated that this effect could be related to the Alrp enzymatic activity.
authors.affiliation: Section of Gastroenterology, Department of Emergency and Organ Transplantation (DETO), University of Bari, Bari, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Polimeno
authors.forename: Barbara
authors.initials: B
authors.lastname: Pesetti
authors.forename: Thomas
authors.initials: T
authors.lastname: Lisowsky
authors.forename: Florenzo
authors.initials: F
authors.lastname: Iannone
authors.forename: Leonardo
authors.initials: L
authors.lastname: Resta
authors.forename: Floriana
authors.initials: F
authors.lastname: Giorgio
authors.forename: Rosanna
authors.initials: R
authors.lastname: Mallamaci
authors.forename: Maura
authors.initials: M
authors.lastname: Buttiglione
authors.forename: Daniela
authors.initials: D
authors.lastname: Santovito
authors.forename: Francesco
authors.initials: F
authors.lastname: Vitiello
authors.forename: M Elisabetta
authors.initials: ME
authors.lastname: Mancini
authors.forename: Antonio
authors.initials: A
authors.lastname: Francavilla
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003579:Cytochrome Reductases / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008933:Mitochondrial Swelling / Q000187:drug effects; D009447:Neuroblastoma / Q000201:enzymology / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D016877:Oxidants / Q000633:toxicity*; D050862:Oxidoreductases Acting on Sulfur Group Donors; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D016877:Oxidants; D011506:Proteins; D011994:Recombinant Proteins; C088825:GFER protein, rat; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D003579:Cytochrome Reductases; C500897:GFER protein, human; D050862:Oxidoreductases Acting on Sulfur Group Donors