Turn-over of meningeal and perivascular macrophages in the brain of MCP-1-, CCR-2- or double knockout mice.
Perivascular and meningeal macrophages are important for immune surveillance in the healthy and the injured brain. Monocyte chemoattractant protein-1 (MCP-1) regulates macrophage migration and permeability of the blood brain barrier. In the present study, we investigated the influence of MCP-1 or/and chemokine receptor 2 (CCR2)-deficiency on macrophage turnover. The results showed no influence of single MCP-1- or CCR-2-deficiency, but double-deficient mice revealed a virtual absence of blood-borne macrophage recruitment. This finding emphasizes that the MCP-1/CCR2 axis is crucially important for macrophage turnover and compensatory mechanisms remain only partially sufficient to sustain regulatory functions.
Schilling
Matthias
M
Department of Neurology, University Hospital Münster, Münster, Germany. schillim@uni-muenster.de
Strecker
Jan-Kolja
JK
Ringelstein
E Bernd
EB
Kiefer
Reinhard
R
Schäbitz
Wolf-Rüdiger
WR
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D018932:Chemokine CCL2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008264:Macrophages / Q000378:metabolism*; D008578:Meninges / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054390:Receptors, CCR2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
D000943:Antigens, Differentiation; C515335:Ccr2 protein, mouse; D018932:Chemokine CCL2; D054390:Receptors, CCR2; C036763:monocyte-macrophage differentiation antigen; D049452:Green Fluorescent Proteins