title: Further studies on a human intestinal bacterium Ruminococcus sp. END-1 for transformation of plant lignans to mammalian lignans.
abstract: A human intestinal bacterium Ruminococcus (R.) sp. END-1 capable of oxidizing (-)-enterodiol to (-)-enterolactone, enantioselectively, was further investigated from the perspective of transformation of plant lignans to mammalian lignans; A cell-free extract of the bacterium transformed (-)-enterodiol to (-)-enterolactone through an intermediate, enterolactol. The bacterium showed not only oxidation but also demethylation and deglucosylation activities for plant lignans. Arctiin and secoisolariciresinol diglucoside were converted to (-)-dihydroxyenterolactone and (+)-dihydroxyenterodiol, respectively. Moreover, by coincubation with Eggerthella sp. SDG-2, the bacterium transformed arctiin and secoisolariciresinol diglucoside to (-)-enterolactone and (+)-enterodiol, respectively.
authors.affiliation: Institute of Natural Medicine, University of Toyama, Sugitani, Toyama, Japan.
authors.forename: Jong-Sik
authors.initials: JS
authors.lastname: Jin
authors.forename: Masao
authors.initials: M
authors.lastname: Hattori
mesh_terms: D001711:Biotransformation; D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism; D005663:Furans / Q000737:chemistry / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D017705:Lignans / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D045857:Ruminococcus / Q000302:isolation & purification / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D002072:Butylene Glycols; D005663:Furans; D005960:Glucosides; D017705:Lignans; C090142:secoisolariciresinol diglucoside; C077992:arctiin