title: Differential Shh, Bmp and Wnt gene expressions during craniofacial development in mice.
abstract: In this study, Bmp-4, Wnt-5a and Shh gene expressions were compared during early craniofacial development in mice by comparative non-isotopic in situ hybridization. Wild-type C57BL/6J mice were studied at various stages of embryonic development (from 8.5- to 13.5-day-old embryos--E8.5-13.5). During early odontogenesis, transcripts for Bmp-4, Shh and Wnt-5a were co-localised at the tooth initiation stage. At E8.5, Shh mRNA expression was restricted to diencephalon and pharyngeal endoderm. Before maxillae and mandible ossification, Bmp-4 and Wnt-5a signals were detected in the mesenchymal cells and around Meckel's cartilage. During palatogenesis, Shh was expressed only in the epithelium and Wnt-5a only in the mesenchyme of the elevating palatal shelves. During tongue development, Shh expression was found in mesenchyme, probably contributing to tongue miogenesis, while Wnt-5a signal was in the epithelium, possibly during placode development and papillae formation. Taken together, these findings suggest that Bmp-4, Shh and Wnt-5a gene expressions may act together on the epithelial-mesenchymal interactions occurring in several aspects of the early mouse craniofacial development, such as odontogenesis, neuronal development, maxillae and mandible ossification, palatogenesis and tongue formation.
authors.affiliation: Department of Oral Pathology, Dental School, University of São Paulo, São Paulo, Brazil. katipaiva@yahoo.com.br
authors.forename: Katiucia Batista Silva
authors.initials: KB
authors.lastname: Paiva
authors.forename: Maria das Graças
authors.initials: Md
authors.lastname: Silva-Valenzuela
authors.forename: Silvia Maria Gomes
authors.initials: SM
authors.lastname: Massironi
authors.forename: Gui Mi
authors.initials: GM
authors.lastname: Ko
authors.forename: Filipe Modolo
authors.initials: FM
authors.lastname: Siqueira
authors.forename: Fabio Daumas
authors.initials: FD
authors.lastname: Nunes
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics*; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism*; D004707:Endoderm / Q000196:embryology / Q000378:metabolism; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D005145:Face / Q000196:embryology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D053823:Hedgehog Proteins / Q000235:genetics*; D007568:Jaw / Q000196:embryology / Q000378:metabolism; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009055:Mouth / Q000196:embryology / Q000378:metabolism; D009297:Nasal Mucosa / Q000378:metabolism; D009666:Nose / Q000196:embryology; D010159:Palate / Q000196:embryology / Q000378:metabolism; D012886:Skull / Q000196:embryology* / Q000378:metabolism; D014059:Tongue / Q000196:embryology / Q000378:metabolism; D014070:Tooth / Q000196:embryology / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics*; D000071818:Wnt-5a Protein
chemical_list: D055415:Bone Morphogenetic Protein 4; D053823:Hedgehog Proteins; C465570:Shh protein, mouse; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C438615:Wnt5a protein, mouse