Cytotoxic effects of calcium hydroxide and mineral trioxide aggregate on 3T3 fibroblast cell line in vitro.
OBJECTIVE
To examine the cytotoxic effects of MTA and Ca(OH)2 on 3T3 fibroblasts at different time intervals.


METHODS
Confluent cells were cultured with Ca(OH)2 and MTA in six-well plates. Wells with only fibroblasts served as controls. Cell number and viability were determined after 24 and 48 hours and 7 days of incubation. For cell viability, trypane blue exclusion assay was used. A Leitz inverted microscope was used to observe the morphological behavior of cells. The proliferation of cells was evaluated by BrdU assay. The results were analyzed with Dunn's multiple comparison, Friedman, and Kruskal-Wallis tests.


RESULTS
No difference was seen in morphology of cells for either test material. Cells treated with MTA and Ca(OH)2 were reduced in number after 24 and 48 hours, respectively. A statistically significant difference was found in number of viable cells between test groups at 48 hours of incubation. The results of BrdU assay revealed low percentages of capable cells incorporating BrdU.


CONCLUSIONS
The cytotoxic effects of the test materials-MTA and Ca(OH)2-on 3T3 cell line were evaluated as cytostatic for 24 and 48 hours, respectively. But this effect was reversible because the incubated cells showed normal cell proliferation at 48 hours and 7 days, respectively; MTA showed a significantly shorter cytotoxic effect on the cells.
Sepet
Elif
E
Instanbul University, Dental Faculty, Department of Pedodontics, Istanbul, Turkey. elifsepet@hotmail.com
Pinar
Arzu
A
Ilhan
Banu
B
Ulukapi
Isin
I
Bilir
Ayhan
A
Tuna
Sevilcan
S
D016475:3T3 Cells / Q000187:drug effects*; D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D007150:Immunohistochemistry; D051379:Mice; D010087:Oxides / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D013997:Time Factors
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D002126:Calcium Hydroxide