Cytotoxicity of polymerized resin cements on human dental pulp cells in vitro.
OBJECTIVE
This study was designed to evaluate the cytotoxicity of several resin-based cements (Panavia F, Super-Bond C&B, Chemiace II) after polymerization on cultured human dental pulp cells.


METHODS
After polymerization, specimens from three resin-based cements were eluted with fresh Dulbecco's modified Eagle's medium (DMEM) without serum for 72 h, at 37 degrees C, using 0.4 g of each substance per milliliter of fresh medium. Elutes obtained during this step were passed through a 0.22-microm filter and diluted with the culture medium by a ratio of 75%, 50%, 25% (v/v). Cytotoxicity of elutes were evaluated by the relative growth rates (RGR) of pulp cells with a modified 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The RGR of pulp cells were statistic analyzed by the one-way analysis of variance among the groups.


RESULTS
The RGR of cells exposed to 100% concentration of elution of Panavia F, Super-Bond C&B, and Chemiace II were 74.42%, 85.54%, and 82.39%, respectively. The RGR increased along with the elution of cements diluted. There was significant difference between the Panavia F group and Super-Bond C&B group (p<0.01), but there was no significant difference in the cytotoxicity between Chemiace II and Super-Bond C&B.


CONCLUSIONS
After polymerization, three resin-based cements (Panavia F, Super-Bond C&B, Chemiace II) induced slight cytotoxicity. The sensitivity of cytotoxicity to human pulp cells depended on the resin-based cements and the concentration of the elution. Super-Bond C&B is the least cytotoxic agent among the three resin-based cements.
Kong
Ninghua
N
Department of Prosthodontics, Peking University, School and Hospital of Stomatology, Beijing 100081, China.
Jiang
Ting
T
Zhou
Zongcan
Z
Fu
Juanling
J
D000293:Adolescent; D000328:Adult; D001896:Boron Compounds / Q000737:chemistry; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D004396:Coloring Agents; D016895:Culture Media, Serum-Free; D003738:Dental Cements / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008768:Methylmethacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry / Q000633:toxicity*; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D055815:Young Adult
D001896:Boron Compounds; D004396:Coloring Agents; D016895:Culture Media, Serum-Free; D003738:Dental Cements; D008689:Methacrylates; D008768:Methylmethacrylates; C438843:Panavia-Fluoro; D011108:Polymers; D019279:Resin Cements; D013778:Tetrazolium Salts; D013844:Thiazoles; C539697:chemiace II; C049495:Super-bond; C022616:thiazolyl blue