title: Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223).
abstract: Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3(+) cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Seon-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Kang
authors.forename: Hee Yong
authors.initials: HY
authors.lastname: Chung
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.forename: Yong-Pil
authors.initials: YP
authors.lastname: Cheon
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Dong-Mok
authors.initials: DM
authors.lastname: Lee
authors.forename: Jongsun
authors.initials: J
authors.lastname: Park
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Taehoon
authors.initials: T
authors.lastname: Chun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D003001:Cloning, Molecular; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D000093322:Lymphocyte Activation Gene 3 Protein
chemical_list: D015703:Antigens, CD; D012313:RNA; D000093322:Lymphocyte Activation Gene 3 Protein