Anti-epidermal growth factor receptor (anti-EGFR) antibody conjugated fluorescent nanoparticles probe for breast cancer imaging.
Fluorescent nanoparticles (FNs) with unique optical properties may be useful as biosensors in living cancer cell imaging and cancer targeting. In this study, anti-EGFR antibody conjugated fluorescent nanoparticles (FNs) (anti-EGFR antibody conjugated FNs) probe was used to detect breast cancer cells. FNs with excellent character such as non-toxicity and photostability were first synthesized with a simple, cost-effective and environmentally friendly modified Stober synthesis method, and then successfully modified with anti-EGFR antibody. This kind of fluorescence probe based on the anti-EGFR antibody conjugated FNs has been used to detect breast cancer cells with fluorescence microscopy imaging technology. The experimental results demonstrate that the anti-EGFR antibody conjugated FNs can effectively recognize breast cancer cells and exhibited good sensitivity and exceptional photostability, which would provide a novel way for the diagnosis and curative effect observation of breast cancer cells and offer a new method in detecting EGFR.
Hun
Xu
X
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. hunxu@sohu.com
Zhang
Zhujun
Z
D055910:Antibodies, Immobilized / Q000737:chemistry; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000276:immunology*; D005260:Female; D006801:Humans; D008856:Microscopy, Fluorescence / Q000191:economics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure
D055910:Antibodies, Immobilized; D066246:ErbB Receptors