title: Impaired differentiation potential of human trabecular bone mesenchymal stromal cells from elderly patients.
abstract: OBJECTIVE
  Advances in bone tissue engineering with mesenchymal stromal cells (MSC) as an alternative to conventional orthopedic procedures has opened new horizons for the treatment of large bone defects. Bone marrow (BM) and trabecular bone are both sources of MSC. Regarding clinical use, we tested the potency of MSC from different sources.
  
  
  METHODS
  We obtained MSC from 17 donors (mean age 64.6 years) by extensive washing of trabecular bone from the femoral head and trochanter, as well as BM aspirates of the iliac crest and trochanter. The starting material was evaluated by histologic analysis and assessment of colony-forming unit-fibroblasts (CFU-F). The MSC populations were compared for proliferation and differentiation potential, at RNA and morphologic levels.
  
  
  RESULTS
  MSC proliferation potential and immunophenotype (expression of CD49a, CD73, CD90, CD105, CD146 and Stro-1) were similar whatever the starting material. However, the differentiation potential of MSC obtained by bone washing was impaired compared with aspiration; culture-amplified cells showed few Oil Red O-positive adipocytes and few mineralized areas and formed inconsistent Alcian blue-positive high-density micropellets after growth under adipogenic, osteogenic and chondrogenic conditions, respectively. MSC cultured with 1 ng/mL fibroblast growth factor 2 (FGF-2) showed better differentiation potential.
  
  
  CONCLUSIONS
  Trabecular bone MSC from elderly patients is not good starting material for use in cell therapy for bone repair and regeneration, unless cultured in the presence of FGF-2.
authors.affiliation: Department of Orthopedic Surgery, University Hospital, Tours, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Coipeau
authors.forename: Philippe
authors.initials: P
authors.lastname: Rosset
authors.forename: Alain
authors.initials: A
authors.lastname: Langonne
authors.forename: Julien
authors.initials: J
authors.lastname: Gaillard
authors.forename: Bruno
authors.initials: B
authors.lastname: Delorme
authors.forename: Angélique
authors.initials: A
authors.lastname: Rico
authors.forename: Jorge
authors.initials: J
authors.lastname: Domenech
authors.forename: Pierre
authors.initials: P
authors.lastname: Charbord
authors.forename: Luc
authors.initials: L
authors.lastname: Sensebe
mesh_terms: D050156:Adipogenesis; D000368:Aged; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010641:Phenotype; D013234:Stem Cells; D017154:Stromal Cells / Q000166:cytology*