title: Shelf life, dissolving action, and antibacterial activity of a neutralized 2.5% sodium hypochlorite solution.
abstract: OBJECTIVE
  The aim was to evaluate the shelf life and the dissolving and antibacterial properties of a neutralized 2.5% NaOCl solution.
  
  
  METHODS
  The loss of available chlorine and the pH of the neutralized 2.5% NaOCl solution were recorded to determine its shelf life. The dissolving action on bovine dental pulp was assessed measuring weight loss, pH variation, and decrease in available chlorine content. The antibacterial activity was evaluated on artificially infected human teeth. The roots were endodontically prepared, sterilized, and inoculated with Enterococcus faecalis before irrigation with the neutralized solution. The presence of intracanal bacteria after irrigation was recorded.
  
  
  RESULTS
  The neutralized solution presented a shelf life of 2 hours, dissolving capacities equivalent to control for the first 5 minutes, and a better antibacterial efficiency.
  
  
  CONCLUSIONS
  The neutralized 2.5% NaOCl solution must be used within 2 hours after mixing, should be frequently renewed to maintain its dissolving capacities, and presented enhanced antibacterial properties.
authors.affiliation: Laboratoire IMEB, UFR Odontologie, Marseille, France. jcamps2035@AOL.com
authors.forename: Jean
authors.initials: J
authors.lastname: Camps
authors.forename: Ludovic
authors.initials: L
authors.lastname: Pommel
authors.forename: Virginie
authors.initials: V
authors.lastname: Aubut
authors.forename: Bernard
authors.initials: B
authors.lastname: Verhille
authors.forename: Fukuzaki
authors.initials: F
authors.lastname: Satoshi
authors.forename: Bernad
authors.initials: B
authors.lastname: Lascola
authors.forename: Imad
authors.initials: I
authors.lastname: About
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D003782:Dental Pulp / Q000187:drug effects*; D003786:Dental Pulp Cavity / Q000187:drug effects* / Q000382:microbiology; D004356:Drug Storage; D013293:Enterococcus faecalis / Q000187:drug effects; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012388:Root Canal Irrigants / Q000737:chemistry / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite