[Effect of calmodulin antagonist O-4-ethoxyl- butyl-berbamine on the proliferation of human breast cancer cell line MCF-7 and its relevant mechanism].
OBJECTIVE
To investigate the effect of calmodulin antagonist O-(4-ethoxyl-butyl)-berbamine (EBB) on proliferation of human breast cancer cell MCF-7 and its possible mechanism.


METHODS
MTT assay was used to analyze the effect of EBB on tumor cells growth. Flow cytometry was used to detect its impact on the cell cycle of MCF-7 cells. Immunofluoresce labeling technique and laser scanning confocal microscope were used to reveal the changes of the microtubule, microfilament, mitochondrion, and endoplasmic reticulum in the cells.


RESULTS
The IC50 value of EBB in MCF-7 cells was (13.0 +/- 3.7) micromol/L. MCF-7 cells were arrested at S phase after EBB treatment. Meanwhile, depolymerization of the microtubule and microfilament, impairment of the mitochondrion and swelling of endoplasmic reticulum were observed.


CONCLUSIONS
EBB arrests MCF-7 cells at S phase by inhibiting the growth of MCF-7 cells, which may be related to the changes of structures and functions of the microtubule, microfilament, mitochondrion, and endoplasmic reticulum.
Dai
Xiao-Hua
XH
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
Liu
Rong
R
Zhou
Yuan
Y
Yang
Ming
M
Fan
Dong-Mei
DM
Xiong
Dong-Sheng
DS
Qi
Jing
J
Yang
Chun-Zheng
CZ
Zhu
Hui-Fang
HF
D044182:Benzylisoquinolines / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans
D044182:Benzylisoquinolines; D002147:Calmodulin; C050387:O-(4-ethoxybutyl)berbamine