Dietary conjugated linoleic acid modifies the brain endocannabinoid system in mice.
Conjugated linoleic acid (CLA) has a role of biogenic regulation through modifying prostaglandin production. However, its effects on related metabolites of arachidonate remain unclear. Therefore, the effects of CLA on brain endocannabinoid content as well as its analogs were investigated. Mice (3-week-old), provided with diets containing 3% linoleic acid or 3% CLA for 4 weeks, were sacrificed and lipids were extracted from their cerebral cortex and hypothalamus. The amounts of N-arachidonoyl-ethanolamide, 2-arachidonoyl-glycerol (2-AG), oleoyl-ethanolamide and palmitoyl-ethanolamide were determined quantitatively by LC-MS. The 2-AG level in the cerebral cortex was significantly decreased by CLA treatment, but the other compounds were unaffected in the cerebral cortex and hypothalamus. The present study indicated that dietary CLA site-selectively decreases 2-AG in the cerebral cortex.
Tsuyama
Shoichiro
S
Laboratory of Advanced Animal and Marine Bioresources, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Oikawa
Daichi
D
Tsuji
Yukiko
Y
Akimoto
Yoriko
Y
Jikuya
Hiroyuki
H
Furuse
Mitsuhiro
M
D000284:Administration, Oral; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D063385:Cannabinoid Receptor Modulators / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D063388:Endocannabinoids; D005989:Glycerides / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D019787:Linoleic Acid / Q000494:pharmacology*; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice
D001095:Arachidonic Acids; D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids; D005989:Glycerides; D044243:Linoleic Acids, Conjugated; C094503:glyceryl 2-arachidonate; D019787:Linoleic Acid