Interaction between estradiol and follicle-stimulating hormone promotes in vitro survival and development of caprine preantral follicles.
The aim of this study was to investigate the effects of estradiol and follicle-stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) containing estradiol (1, 5, 10, 20 or 40 pg/ml), FSH (50 ng/ml), or a combination of the two hormones. Cultured and noncultured control ovarian tissues were processed for histological and ultrastructural studies. The results showed that after 7 days of culture, the treatments that yielded the highest percentage of normal follicles relative to MEM alone were those that combined FSH with estradiol at 1, 5 or 20 pg/ml. The addition of FSH to 1-day cultures containing 1 pg/ml estradiol or to 7-day cultures with 1 or 5 pg/ml estradiol increased the percentage of normal follicles compared to estradiol alone at the same concentrations. After 7 days of culture, all treatments generated higher percentages of developing follicles as compared to control and MEM alone. The addition of either FSH or 10 pg/ml of estradiol to the culture media or estradiol (1, 5, 10 or 20 pg/ml) and FSH in combination significantly increased follicular diameter as compared with MEM alone following 7 days of culture. Ultrastructural studies confirmed follicular integrity after 7 days of culture in the presence of 1 pg/ml estradiol plus FSH. In conclusion, this study demonstrated that the interaction between estradiol and FSH maintains ultrastructural integrity and stimulates activation and further growth of cultured caprine preantral follicles.
Lima-Verde
I B
IB
Northeast Network of Biotechnology, Fortaleza, Brazil. isabel_limaverde@yahoo.com.br
Matos
M H T
MH
Saraiva
M V A
MV
Bruno
J B
JB
Tenório
S B
SB
Martins
F S
FS
Rossetto
R
R
Cunha
L D
LD
Name
K P O
KP
Báo
S N
SN
Campello
C C
CC
Figueiredo
J R
JR
D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage* / Q000737:chemistry; D006041:Goats; D006728:Hormones / Q000008:administration & dosage* / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D013997:Time Factors
D003470:Culture Media; D006728:Hormones; D004958:Estradiol; D005640:Follicle Stimulating Hormone