Sequence-specific 1H, 13C, and 15N backbone assignment of the activated 21 kDa GTPase rRheb.
Ras homologue enriched in brain (Rheb) is a small GTPase that plays an important role in tuberous sclerosis. Here we present the backbone assignments of activated rRheb in complex with the non-hydrolisable GTP analogue GppNHp. These assignments now provide a basis for the analysis of rRheb's interaction with putative effectors in order to further elucidate the physiological function of this GTPase and its role in the regulation of neuronal cell volume as well as in tuberous sclerosis.
Schwarten
Melanie
M
Biomolecular NMR, Ruhr-University of Bochum, Bochum 44780, Germany.
Berghaus
Carsten
C
Heumann
Rolf
R
Stoll
Raphael
R
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D004789:Enzyme Activation; D006153:Guanosine Diphosphate / Q000378:metabolism; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D015394:Molecular Structure; D020559:Monomeric GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D000076205:Ras Homolog Enriched in Brain Protein; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D009479:Neuropeptides; D000076205:Ras Homolog Enriched in Brain Protein; D011994:Recombinant Proteins; C087693:Rheb protein, rat; D006153:Guanosine Diphosphate; D006165:Guanylyl Imidodiphosphate; D020559:Monomeric GTP-Binding Proteins