[Anti-tumor effects on human laryngeal carcinoma Hep-2 of recombinant fowlpox virus expressing chicken anemia virus Apoptin gene].
OBJECTIVE
To investigate the anti-tumor effects and the mechanism of the recombinant fowlpox virus expressing Apoptin gene on human laryngeal carcinoma Hep-2.


METHODS
Hep-2 cells cultured in vitro were infected with vFVApoptin. The anti-tumor effects on Hep-2 cells were measured through MTT staining and, the mitochondrial trans-membrane potential (delta psi m) and reactive oxygen species (ROS) were analyzed by flow cytometry. Western blot was used to detect the release of cytochrome c (Cyto c). Caspase-3/9 activities were measured by colorimetric assay.


RESULTS
vFVApoptin could restrain Hep-2 cells significantly and, had the function of down-regulating delta psi m, up-regulating ROS, promoting Cyto c release and activating Caspase-3/9.


CONCLUSIONS
Cyto c were released from mitochondria by the function of up-regulating ROS of vFVApoptin. Cyto c triggered Caspase-9 and, after the activation of Caspase-9, downstream apoptotic factors, such as caspase-3, were activated. Eventually, Hep-2 cells were suppressed by mitochondrial pathway apoptosis induced by vFVApoptin.
Guan
Guofang
G
Department of Otorhinolaryngology, the Second Hospital, Bethune Faculty of Medical Sciences of Jilin University, Changchun, 130041, China. guan-guo@163.com
Jin
Ningyi
N
Li
Xiao
X
Sun
Lili
L
Jin
Chunshun
C
Lou
Wei
W
Shi
Ping
P
Hao
Yanru
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D036022:Capsid Proteins / Q000235:genetics / Q000494:pharmacology*; D017727:Chicken anemia virus / Q000235:genetics; D005587:Fowlpox virus / Q000235:genetics; D006801:Humans; D014407:Tumor Cells, Cultured
D036022:Capsid Proteins; C091717:VP3 protein, Chicken anemia virus