Rapid separation of three glucosylated resveratrol analogues from the invasive plant Polygonum cuspidatum by high-speed countercurrent chromatography.
Three glucosylated resveratrol analogues (piceid, piceatannol glucoside, resveratroloside) were successfully isolated from the crude MeOH extract of the invasive plant species Polygonum cuspidatum by semi-preparative high-speed countercurrent chromatography with a two-phase solvent system composed of cyclohexane-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v). Piceid (23 mg), resveratroloside (17 mg), piceatannol glucoside (15 mg) of purities over 80% were isolated from 500 mg crude MeOH extract in one step. Subsequent passage over a SPE column was used to quickly bring their purities to over 90%. The purities were determined by HPLC analysis and their structures were elucidated by proton nuclear magnetic resonance ((1)H-NMR), HMBC, ESI-MS and HR-MS.
Fan
Peihong
P
Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest Ansermet, Geneva, Switzerland.
Marston
Andrew
A
Hay
Anne-Emmanuelle
AE
Hostettmann
Kurt
K
D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D000077185:Resveratrol; D012997:Solvents / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry*
D005960:Glucosides; D010936:Plant Extracts; D012997:Solvents; D013267:Stilbenes; D000077185:Resveratrol