Expression of heat shock proteins and nitrotyrosine in small arteries from patients with coronary heart disease.
Heat shock proteins (HSPs) have been suggested to play an important role in the pathogenesis of cardiovascular disease; however, their levels in resistance arteries and their role as useful markers for endothelial dysfunction are not well known. In this paper we studied the levels of HSP90, HSP70, HSP60, HSP27, and of the oxidative stress marker nitrotyrosine (NT) in isolated small subcutaneous arteries from female and male patients with coronary heart disease (CHD) and compared them with healthy controls. HSPs and NT levels were analyzed by immunohistochemistry (IHC) with streptavidin-biotin complex and 3,3'-diaminobenzidine (DAB) staining. The results were assessed with a semi-quantitative method. The study showed lower levels of HSP90 in arteries from both male and female patients when compared to the healthy controls, while levels of HSP70 were lower only in male patients versus controls. The levels of HSP60 and HSP27 did not show any significant difference in either the male or the female groups. NT levels were higher in the arteries from female patients as compared to controls. In conclusion, the present study strengthens the concept that HSPs may play an important role in the pathogenesis of CHD, and that at least two of them, HSP70 and HSP90, may have useful applications as markers of vascular dysfunction in resistance arteries.
Paier
Anton
A
Institution for Clinical Science Intervention and Technology , Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge Campus, Stockholm 14186, Sweden.
Agewall
Stefan
S
Kublickiene
Karolina
K
D000328:Adult; D000368:Aged; D001158:Arteries / Q000737:chemistry; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000032:analysis; D003324:Coronary Artery Disease / Q000378:metabolism*; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D018384:Oxidative Stress; D050151:Subcutaneous Fat / Q000098:blood supply*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D018834:Chaperonin 60; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; C002744:3-nitrotyrosine; D014443:Tyrosine