A new and efficient method for inhibition of RNA viruses by DNA interference.
We report here a new method for inhibition of RNA viruses induced by dsDNA. We demonstrated that both long dsDNA molecules and short interfering DNA with a sequence complementary to that of viral RNA inhibited tobacco mosaic virus expression and prevented virus spread. Also, the expression of the HIV-1 gp41 gene in HeLa cells was inhibited by complementary short interfering DNA. We showed that Dicer processed dsDNA, which suggests activation of the cellular machinery involved in silencing of RNA. For the silencing of viral RNA effected with dsDNA, we coined the term DNA interference technology.
Nowak
Monika
M
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Wyszko
Eliza
E
Fedoruk-Wyszomirska
Agnieszka
A
Pospieszny
Henryk
H
Barciszewska
Mirosława Z
MZ
Barciszewski
Jan
J
D053487:DEAD-box RNA Helicases / Q000502:physiology; D016373:DNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D020868:Gene Silencing / Q000187:drug effects*; D005814:Genes, Viral / Q000187:drug effects*; D015700:HIV Envelope Protein gp41 / Q000037:antagonists & inhibitors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008722:Methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D012328:RNA Viruses / Q000187:drug effects* / Q000235:genetics; D012367:RNA, Viral / Q000037:antagonists & inhibitors*; D043244:Ribonuclease III / Q000502:physiology; D014027:Tobacco Mosaic Virus / Q000187:drug effects / Q000235:genetics
D016373:DNA, Antisense; D015700:HIV Envelope Protein gp41; D020319:Oligodeoxyribonucleotides, Antisense; D012367:RNA, Viral; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases