[Noninvasive prenatal diagnosis of Hb Bart's hydrops fetus using cell-free fetal DNA in maternal plasma].
OBJECTIVE
To explore a new noninvasive method for Hb Bart' s hydrops fetus by using PCR amplification efficiency discrimination between cell-free fetal DNA (cffDNA) and cell-free maternal DNA in maternal plasma.


METHODS
CffDNA samples from pregnant women bearing possible Hb Bart's hydrops fetus were collected. Fluorescent PCR and capillary electrophoresis (CE) were performed. Hb Bart's hydrops fetus was conclusively identified by different peak area ratio of products.


RESULTS
The peak area ratio of 30 cffDNA samples from Hb Bart' s hydrops fetus was much less than 1. However, the ratio of cffDNA sample from hydrops fetus due to other reasons was approximately equal to 1.


CONCLUSIONS
By using cffDNA fluorescent PCR and CE, a prenatal screening method for Hb Bart' s hydrops fetus was developed.
Long
Xing-Jiang
XJ
Department of Pediatrics, Fifth Affiliated Hospital of Guangxi Medical University, Liuzhou People Hospital, Liuzhou 530021, China.
Long
Gui-Fang
GF
Lin
Wei-Xiong
WX
D001477:Bartter Syndrome / Q000175:diagnosis*; D004247:DNA / Q000097:blood*; D005260:Female; D006801:Humans; D015160:Hydrops Fetalis / Q000175:diagnosis*; D008969:Molecular Sequence Data; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011296:Prenatal Diagnosis / Q000379:methods
D004247:DNA