title: Reassessment of microarray expression data of porokeratosis by quantitative real-time polymerase chain reaction.
abstract: BACKGROUND
  Porokeratosis (PK) is a heterogeneous group of keratinization disorders that exhibit similarities with psoriasis at both the clinical and molecular levels.
  
  
  METHODS
  The transcript levels of keratin (KRT) 6A, 16, 17, S100A7, A8, A9, p53 and three candidate genes (i.e. SART3, SSH1 and ARPC3) were reassessed in pairwise lesional and uninvolved skin from nine patients with PK by real-time quantitative polymerase chain reaction (RTQ-PCR).
  
  
  RESULTS
  The results of RTQ-PCR confirmed that KRT6A, 16, S100A7, A8 and A9 (p = 0.008) were mostly up-regulated in the lesional skin when compared with uninvolved skin. Different from the microarray data, there was no significant difference observed in KRT17 expression patterns between lesional and normal-appearing skin (p = 0.066). No statistical difference was observed in p53 and three candidate genes' expression patterns between lesional and uninvolved skin.
  
  
  CONCLUSIONS
  In the present study, 9 of the 10 gene expression measured by RTQ-PCR in PK were statistically comparable to microarray data. KRT6A was identified as specific biomarker for porokeratotic keratinocytes, as it was the most significantly up-regulated gene in the nine patient samples.
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China. verzhang@gmail.com
authors.forename: Zheng-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Meredith E
authors.initials: ME
authors.lastname: Crosby
authors.forename: Ke-Fei
authors.initials: KF
authors.lastname: Kang
authors.forename: Jing
authors.initials: J
authors.lastname: Luan
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Lei-Hong
authors.initials: LH
authors.lastname: Xiang
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Zheng
mesh_terms: D017668:Age of Onset; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017499:Porokeratosis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*; D046888:Tissue Array Analysis
chemical_list: D012333:RNA, Messenger