title: Physical and functional interaction between heterochromatin protein 1alpha and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U.
abstract: By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1alpha (HP1alpha) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1alpha-interacting molecule. More importantly, hnRNP U depletion reduces HP1alpha-dependent gene silencing and disturbs HP1alpha subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1alpha function, shedding new light on the mode of action of HP1alpha and on the function of hnRNP U.
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France.
authors.forename: Maya
authors.initials: M
authors.lastname: Ameyar-Zazoua
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France.
authors.forename: Mouloud
authors.initials: M
authors.lastname: Souidi
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France.
authors.forename: Lauriane
authors.initials: L
authors.lastname: Fritsch
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Robin
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Thomas
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Hamiche
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, S-171 77 Stockholm, Sweden.
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Percipalle
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France.
authors.forename: Slimane
authors.initials: S
authors.lastname: Ait-Si-Ali
authors.affiliation: CNRS FRE 2944, Institut André Lwoff; Université Paris-Sud, Villejuif F-94801, France. Electronic address: ahbellan@vjf.cnrs.fr.
authors.forename: Annick
authors.initials: A
authors.lastname: Harel-Bellan
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D034722:Heterogeneous-Nuclear Ribonucleoprotein U / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D046912:Multiprotein Complexes; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C000715594:CBX5 protein, human; D002868:Chromosomal Proteins, Non-Histone; D034722:Heterogeneous-Nuclear Ribonucleoprotein U; D046912:Multiprotein Complexes; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D000090266:Chromobox Protein Homolog 5