Mediator-assisted rhodamine B decolorization by Tramates versicolor laccase.
This study reported the decolorization of hazardous xanthenes dye, Rhodamine B by the Laccase Mediator System (LMS). Seven redox mediators were investigated in the mediators-assisted lacase catalyze oxidation reactions. Among redox mediators tested, 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was the best one which gave the highest Rhodamine B decolorization for more than 80% within 48 h while only 20% achieved with no mediator added. In the laccase-ABTS mediator system, the best molar ratio of dye/mediator was 1:10 and dye/enzyme ratio was 0.5 micromol U(-1). The optimum conditions for Rhodamine B decolorization were at pH 4.0-5.0 and temperature 35-40 degrees C. The laccase-ABTS system could be a promising biotechnology developed for treatment of textile waste waters containing Rhodamine B.
Khammuang
S
S
Protein and Enzyme Technology Research Unit, Department of Chemistry, Faculty of Science, Mahasarakham University, T. Khamriang, A. Kantarawichai, Maha Sarakham, 44150 Thailand.
Sarnthima
R
R
D052160:Benzothiazoles / Q000378:metabolism; D003116:Color; D004396:Coloring Agents / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D007202:Indicators and Reagents / Q000378:metabolism; D007220:Industrial Waste; D042845:Laccase / Q000378:metabolism*; D010084:Oxidation-Reduction; D012235:Rhodamines / Q000378:metabolism*; D013451:Sulfonic Acids / Q000378:metabolism; D055454:Trametes / Q000201:enzymology*
D052160:Benzothiazoles; D004396:Coloring Agents; D005456:Fluorescent Dyes; D005656:Fungal Proteins; D007202:Indicators and Reagents; D007220:Industrial Waste; D012235:Rhodamines; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D042845:Laccase; C029773:rhodamine B