title: Transplantation of mouse induced pluripotent stem cells into the cochlea.
abstract: This study examined the potential of induced pluripotent stem (iPS) cells for use as a source of transplants for the restoration of auditory spiral ganglion neurons. We monitored neurite outgrowth from iPS cell-derived neural progenitors toward cochlear hair cells ex vivo, and followed their survival and fates after transplantation into mouse cochleae in vivo. Neurons derived from iPS cells projected neurites toward cochlear hair cells. The settlement of iPS cell-derived neurons was observed 1 week after transplantation into the cochlea. Some transplants expressed vesicular glutamate transporter 1, which is a marker for glutamatergic neurons. These findings indicate that iPS cells can be used as a source of transplants for the regeneration of spiral ganglion neurons.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nishimura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ono
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ogita
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Keisuke
authors.initials: K
authors.lastname: Okita
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamanaka
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D003051:Cochlea / Q000601:surgery*; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000502:physiology; D005347:Fibroblasts; D006198:Hair Cells, Auditory / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D016501:Neurites / Q000502:physiology; D055495:Neurogenesis; D009474:Neurons / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000502:physiology* / Q000637:transplantation*; D013136:Spiral Ganglion / Q000166:cytology*; D013997:Time Factors; D050598:Vesicular Glutamate Transport Protein 1 / Q000378:metabolism
chemical_list: D050598:Vesicular Glutamate Transport Protein 1