Molecular characterization and therapeutic potential of a marine bacterium Pseudoalteromonas sp. KMM 701 alpha-galactosidase.
An alpha-galactosidase capable of converting B red blood cells into the universal blood type cells at the neutral pH was produced by a novel obligate marine bacterium strain KMM 701 (VKM B-2135 D). The organism is heterotrophic, aerobic, and halophilic and requires Na+ ions and temperature up to 34 degrees C for its growth. The strain has a unique combination of polysaccharide-degrading enzymes. Its single intracellular alpha-galactosidase exceeded other glycoside hydrolases in the level of expression up to 20-fold. The alpha-galactosidase was purified to determine the N-terminal amino acid sequences and new activities. It was found to inhibit Corynebacterium diphtheria adhesion to host buccal epithelium cell surfaces with high effectiveness. The nucleotide sequence of the homodimeric alpha-galactosidase indicates that its subunit is composed of 710 amino acid residues with a calculated Mr of 80,055. This alpha-galactosidase shares structural property with 36 family glycoside hydrolases. The properties of the enzyme are likely to be highly beneficial for medicinal purposes.
Balabanova
Larissa A
LA
Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 690022, prospect 100 letya Vladivostoka, 159, Vladivostok, Russian Federation. balaban@piboc.dvo.ru
Bakunina
Irina Yu
IY
Nedashkovskaya
Olga I
OI
Makarenkova
Ilona D
ID
Zaporozhets
Tatiana S
TS
Besednova
Natalia N
NN
Zvyagintseva
Tatiana N
TN
Rasskazov
Valery A
VA
D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000187:drug effects; D003353:Corynebacterium diphtheriae / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D004789:Enzyme Activation; D008969:Molecular Sequence Data; D031161:Pseudoalteromonas / Q000201:enzymology*; D013329:Structure-Activity Relationship; D000519:alpha-Galactosidase / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*
D000519:alpha-Galactosidase