Isolation and purification of D-mannose from palm kernel.
An economically viable procedure for the isolation and purification of d-mannose from palm kernel was developed in this research. The palm kernel was catalytically hydrolyzed with sulfuric acid at 100 degrees C and then fermented by mannan-degrading enzymes. The solution after fermentation underwent filtration in a silica gel column, desalination by ion-exchange resin, and crystallization in ethanol to produce pure d-mannose in a total yield of 48.4% (based on the weight of the palm kernel). Different enzymes were investigated, and the results indicated that endo-beta-mannanase was the best enzyme to promote the hydrolysis of the oligosaccharides isolated from the palm kernel. The pure d-mannose sample was characterized by FTIR, (1)H NMR, and (13)C NMR spectra.
Zhang
Tao
T
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
Pan
Ziguo
Z
Qian
Chao
C
Chen
Xinzhi
X
D003365:Costs and Cost Analysis; D003460:Crystallization; D005285:Fermentation; D005374:Filtration; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007475:Ion Exchange Resins / Q000737:chemistry; D019684:Magnoliopsida / Q000033:anatomy & histology* / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D009930:Organic Chemicals / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D007475:Ion Exchange Resins; D009930:Organic Chemicals; D012997:Solvents; D006026:Glycoside Hydrolases; D008358:Mannose