[An enzyme-mediated mechanism of sodium nitroprusside NO-donating activity in the rat aorta].
The mechanisms of NO-mediated physiological activity of sodium nitroprusside (SNP) have been investigated in isolated rat aorta segments. It was shown, that in pretreated with 1.5 mM NaCN aorta segments SNP causes slight vasoconstriction. Heme-oxidizer K3Fe(CN)6 markedly suppressed SNP-induced vasodilatation. Also it was revealed that NaCN inhibits NO2- accumulation, which was observed in buffer solution with SNP after addition of microsomal membranes isolated from rat aorta homogenates. The great importance of cell membranes heme-containing enzymes for display of SNP NO-donating activity and the possibility of SNP-released CN- involvement in suppression of SNP-induced vasodilatation is discussed.
Iakovenko
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IN
Zhirnov
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VV
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D066298:In Vitro Techniques; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D012966:Sodium Cyanide / Q000494:pharmacology; D012995:Solubility; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects*
D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D006162:Guanylate Cyclase; D012966:Sodium Cyanide