High-performance liquid chromatography coupled with mass spectrometry for the quantitative analysis of vinca-alkaloids in biological matrices: a concise survey from the literature.
The bioanalysis of vinca-alkaloids has been investigated extensively. High-performance liquid chromatography coupled to ultraviolet, fluorescence or electrochemical detection have been described. During recent years liquid chromatography coupled with mass spectrometry (LC-MS) has become the first choice for the quantitative bioanalysis of the vinca anticancer agents. This paper reviews recent methods for the bio-analysis of vinca-alkaloids using LC-MS, supplemented with our own experience. We will focus on sample pre-treatment, chromatography and MS detection and pay attention to problems which can occur during the bioanalysis of vinca-alkaloids. These problems encounter carry-over and absorption effects and solutions will be provided how to circumvent these problems.
Damen
Carola W N
CW
Department of Pharmacy and Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, 1066 EC Amsterdam, The Netherlands. carola.damen@slz.nl
Rosing
Hilde
H
Schellens
Jan H M
JH
Beijnen
Jos H
JH
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D014748:Vinca Alkaloids / Q000032:analysis* / Q000737:chemistry
D014748:Vinca Alkaloids