The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii.
Here we report the characterization of the Chlamydomonas reinhardtii gene ARG9, encoding the plastid resident N-acetyl ornithine aminotransferase, which is involved in arginine synthesis. Integration of an engineered ARG9 cassette in the plastid chromosome of the nuclear arg9 mutant restores arginine prototrophy. This suggests that ARG9 could be used as a new selectable marker for plastid transformation.
Remacle
Claire
C
Department of Life Sciences, Université de Liège, Liège, Belgium.
Cline
Sara
S
Boutaffala
Layla
L
Gabilly
Stéphane
S
Larosa
Véronique
V
Barbieri
M Rosario
MR
Coosemans
Nadine
N
Hamel
Patrice P
PP
D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics / Q000378:metabolism*; D018087:Plastids / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport
D020418:Algal Proteins; D001120:Arginine; D009953:Ornithine-Oxo-Acid Transaminase