The twin spot generator for differential Drosophila lineage analysis.
In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.
Griffin
Ruth
R
Commissariat à l'Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France. rgriffin@receptor.med.harvard.edu
Sustar
Anne
A
Bonvin
Marianne
M
Binari
Richard
R
del Valle Rodriguez
Alberto
A
Hohl
Amber M
AM
Bateman
Jack R
JR
Villalta
Christians
C
Heffern
Elleard
E
Grunwald
Didier
D
Bakal
Chris
C
Desplan
Claude
C
Schubiger
Gerold
G
Wu
C-ting
CT
Perrimon
Norbert
N
D000818:Animals; D019070:Cell Lineage; D002999:Clone Cells; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D005470:Fluorometry; D023281:Genomics / Q000295:instrumentation / Q000379:methods*; D008938:Mitosis; D009154:Mutation; D011995:Recombination, Genetic