title: Processing of semen can result in increased sperm DNA fragmentation.
abstract: Processing of semen for assisted reproductive technologies (ART) entails a number of procedures that include semen liquefaction, removal of seminal plasma by centrifugation, incubation, and cryopreservation. The results of this study indicate that incubation of semen at room temperature and semen cryopreservation can result in increased levels of sperm DNA fragmentation.
authors.affiliation: Institut Marquès, Service of Reproduction, Barcelona, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Toro
authors.forename: Silvia
authors.initials: S
authors.lastname: Fernández
authors.forename: Ana
authors.initials: A
authors.lastname: Colomar
authors.forename: Aïda
authors.initials: A
authors.lastname: Casanovas
authors.forename: Juan G
authors.initials: JG
authors.lastname: Alvarez
authors.forename: Marisa
authors.initials: M
authors.lastname: López-Teijón
authors.forename: Esther
authors.initials: E
authors.lastname: Velilla
mesh_terms: D000328:Adult; D002498:Centrifugation / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D008875:Middle Aged; D027724:Reproductive Techniques, Assisted; D012662:Semen Preservation / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013094:Spermatozoa / Q000473:pathology*; D013696:Temperature