Accurate quantitation of standard peptides used for quantitative proteomics.
MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.
Bordeerat
Narisa K
NK
Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, USA.
Georgieva
Nadia I
NI
Klapper
David G
DG
Collins
Leonard B
LB
Cross
Tyra J
TJ
Borchers
Christoph H
CH
Swenberg
James A
JA
Boysen
Gunnar
G
D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods; D010455:Peptides / Q000032:analysis*; D040901:Proteomics / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods
D010455:Peptides