title: Characterization of Singapore grouper iridovirus (SGIV) ORF086R, a putative homolog of ICP18 involved in cell growth control and virus replication.
abstract: Singapore grouper iridovirus (SGIV), as a causative agent of serious systemic disease, causes significant economic losses in grouper aquaculture. In this study, a novel ICP18 homolog encoded by SGIV ORF086R was identified and characterized. Strikingly, ICP18 homologs can be found in all ranaviruses, but not in other sequenced large DNA viruses. SGIV ICP18 is an immediate-early gene and begins to be transcribed as early as 2 h post-infection (p.i.). Western blotting indicated that SGIV ICP18 is translated as early as 6 h p.i. and is a viral non-envelope protein. Subcellular localization analysis revealed that the SGIV ICP18 displays a finely punctate cytoplasmic pattern. Furthermore, overexpression of SGIV ICP18 can promote the growth of grouper embryonic cells (GP) and contribute to SGIV replication. These results should offer important insights into the pathogenesis of ranaviruses.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, 135 West Xingang Road, 510275, Guangzhou, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Xia
authors.forename: Jianhao
authors.initials: J
authors.lastname: Cao
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Huang
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001492:Bass / Q000821:virology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004266:DNA Virus Infections / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000821:virology*; D005814:Genes, Viral; D017874:Immediate-Early Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017995:Ranavirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014779:Virus Replication
chemical_list: D017874:Immediate-Early Proteins