title: Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates.
abstract: An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate the force of solution flowing through the capillary during the multi-steps immunoassay process. Immunoassay could be performed with many improvements-rapidity per sample as compared to the conventional micro-plate format (40 min vs. 5-8 h); lower cost and simpler as compared to fused silica capillary with covalent immobilization; and without problem of back pressure as compared to flow injection-bead based immunoassay. Performance of the sequential injection-capillary immunoassay was demonstrated by assay of sialoglycoconjugates level in human serum to differentiate cancer patients from healthy people.
authors.affiliation: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. kradtas@yahoo.com
authors.forename: Supaporn Kradtap
authors.initials: SK
authors.lastname: Hartwell
authors.forename: Napaporn
authors.initials: N
authors.lastname: Wannaprom
authors.forename: Prachya
authors.initials: P
authors.lastname: Kongtawelert
authors.forename: Kate
authors.initials: K
authors.lastname: Grudpan
mesh_terms: D000327:Adsorption; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D017022:Flow Injection Analysis / Q000379:methods; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis*; D012795:Sialoglycoproteins / Q000032:analysis*; D055815:Young Adult
chemical_list: D006017:Glycolipids; D012795:Sialoglycoproteins; C009255:sialoglycolipids