[Construction of attenuated Salmonella choleraesuis vaccine strain expressing recombinant antigen of Shiga-like toxin Escherichia coli].
OBJECTIVE
To construct an attenuated Salmonella choleraesuis vaccine strain expressing the gene SLT-IIeB and FedF of Shiga-like toxin Escherichia coli (SLTEC) O138 with balanced lethal system.


METHODS
The gene of SLT-IIeB and FedF of SLTEC O138 was amplified, and then recombined with a vector pYA3493 (Asd+). The recombinant was electroporated into attenuated Salmonella choleraesuis C500 (Asd-). The expression of SLT-IIeB and FedF was analyzed by SDS-PAGE. The stability of the vaccine strains was studied by generation culture in vitro.


RESULTS
The stable attenuated Salmonella choleraesuis vaccine strain expressing SLT-IIeB and FedF of SLTEC O138 was constructed with balanced lethal system. The expressed products with protein quality 37000 could react with the antibody of FedF and SLT-IIeB.


CONCLUSIONS
The attenuated Salmonella choleraesuis vaccine strain could probably serve as a vaccine against edema disease and piglets paratyphoid.
Man
Xiaoying
X
State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan 430070, China. manxiaoying2002@yahoo.com.cn
Wu
Bin
B
Luo
Yong
Y
Yu
Teng
T
Zhao
Zhanqin
Z
Xu
Yindi
Y
Guo
Aizhen
A
Chen
Huanchun
H
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012475:Salmonella / Q000235:genetics* / Q000276:immunology*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000235:genetics* / Q000276:immunology*
D000942:Antigens, Bacterial; D014613:Vaccines, Attenuated