Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells.
The genus Cryptosporidium includes several species of intestinal protozoan parasites which multiply in intestinal epithelial cells. The impact of this infection on the transcriptome of cultured host cells was investigated using DNA microarray hybridizations. The expression of 14 genes found to be consistently up- or down-regulated in infected cell monolayers was validated with RT PCR. Using immunofluorescence we examined the expression of Protease Activated Receptor-2, which is encoded by one of the up-regulated genes. In infected cells this receptor localized to the host cell membrane which covers the intracellular trophozoites and meronts. This observation indicates that the composition of the host cell membrane is affected by the developing trophozoite, a phenomenon which has not been described previously.
Yang
Yi-Lin
YL
Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA.
Serrano
Myrna G
MG
Sheoran
Abhineet S
AS
Manque
Patricio A
PA
Buck
Gregory A
GA
Widmer
Giovanni
G
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D016785:Cryptosporidium parvum / Q000502:physiology*; D004847:Epithelial Cells / Q000469:parasitology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions; D006801:Humans; D008856:Microscopy, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D044464:Receptor, PAR-2 / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D044464:Receptor, PAR-2