Application of poly(dimethylsiloxane)/glass microchip for fast electrophoretic separation of serum small, dense low-density lipoprotein.
Due to the mounting evidence of altered low-density lipoprotein (LDL) size in several disease states, there has been an increasing interest in developing new analytical methods for small, dense low-density lipoprotein (sdLDL) for diagnosis. The present report demonstrates that sdLDL analysis can be performed in a poly(dimethylsiloxane) (PDMS/glass) microchannel. n-Dodecyl beta-D-maltoside (DDM) was utilized to alter channel surface to make it become hydrophilic and nonionic, thus reducing the interaction between the protein and the surface. Moreover, hydroxypropylcellulose (HPC) was added into the running buffer to suppress the adsorption of analytes and also to serve as a sieving matrix. Under optimal conditions, two baseline separations of lipoproteins including high-density lipoprotein (HDL), sdLDL, and lLDL were achieved with different selectivity. LDL particles shown on the electropherogram were also identified by several procedures. This method affords high separation speed and high reproducibility. The intraassay and interassay RSDs of lipoprotein migration times were in the range of 2.01-2.45%. The variation of serum sdLDL of a patient between prior treatment and post-treatment was assessed by this method. This system has the potential for rapid and sensitive detection of different LDL forms, and thus will be applicable to clinical diagnosis.
Wang
Hua
H
Clinical Medical College of Yangzhou University, 98 Nantong West Road, Yangzhou, 225001, China.
Wang
Daxin
D
Wang
Jingcheng
J
Wang
Huimin
H
Gu
Jian
J
Han
Chongxu
C
Jin
Qinghui
Q
Xu
Baojian
B
He
Chao
C
Cao
Li
L
Wang
Ying
Y
Zhao
Jianlong
J
D000368:Aged; D050197:Atherosclerosis / Q000175:diagnosis; D001797:Blood Protein Electrophoresis / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives; D003902:Detergents; D004129:Dimethylpolysiloxanes; D019075:Electrophoresis, Capillary / Q000379:methods*; D005898:Glass; D005960:Glucosides; D006801:Humans; D008074:Lipoproteins / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D046208:Microchip Analytical Procedures; D015203:Reproducibility of Results
D003902:Detergents; D004129:Dimethylpolysiloxanes; D005960:Glucosides; D008074:Lipoproteins; D008077:Lipoproteins, LDL; C013830:baysilon; C040358:dodecyl maltoside; D002482:Cellulose; C008079:hydroxypropylcellulose