title: High affinity binding to profilin by a covalently constrained, soluble mimic of phosphatidylinositol-4,5-bisphosphate micelles.
abstract: Phosphoinositide (PI) lipids are essential regulators of a wide variety of cellular functions. We present here the preparation of a multivalent analogue of a phosphatidylinositol-4,5-bisphosphate (PIP(2)) micelle containing only the polar headgroup portion of this lipid. We show that this dendrimer binds to the cytoskeletal protein profilin with an affinity indistinguishable from that of PIP(2), despite the fact that profilin discriminates between PIP(2) and its monomeric hydrolysis product inositol-1,4,5-triphosphate (IP(3)) under physiological conditions. These data demonstrate that the diacylglycerol (DAG) moiety of PIP(2) is not required for high-affinity binding and suggest that profilin uses multivalency as a key means to distinguish between the intact lipid and IP(3). The class of soluble membrane analogues described here is likely to have broad applicability in the study of protein.PI interactions.
authors.affiliation: Department of Chemistry, Indiana University, 212 South Hawthorne Drive, Bloomington, Indiana 47405, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Richer
authors.forename: Nichole K
authors.initials: NK
authors.lastname: Stewart
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Webb
authors.forename: John W
authors.initials: JW
authors.lastname: Tomaszewski
authors.forename: Martha G
authors.initials: MG
authors.lastname: Oakley
mesh_terms: D050091:Dendrimers; D006801:Humans; D008823:Micelles; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000031:analogs & derivatives* / Q000378:metabolism*; D011073:Polyamines / Q000737:chemistry* / Q000378:metabolism; D051304:Profilins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D050091:Dendrimers; D008823:Micelles; C104700:PAMAM Starburst; D019269:Phosphatidylinositol 4,5-Diphosphate; D011073:Polyamines; D051304:Profilins