title: Dental pulp and periodontal ligament cells support osteoclastic differentiation.
abstract: Odontoclasts and cementoclasts are considered to play major roles in the internal resorption of dentin and the external resorption of tooth roots. In this study, we evaluated the osteoclast-inducing ability of human dental pulp and periodontal ligament cells, which are mesenchymal cells in dental tissues. These cells expressed RANKL and OPG mRNA constitutively. As osteoclast precursors, CD14(+) monocytes derived from human peripheral blood were isolated, and incubated together with human dental pulp or periodontal ligament cells. Both cell types spontaneously induced the differentiation of CD14(+) monocytes into osteoclasts without osteotropic factors. These results suggest that dental pulp and periodontal ligament cells are involved in regulating the differentiation and function of osteoclasts.
authors.affiliation: Graduate School of Oral Medicine, Institute for Oral Science, Matsumoto Dental University, 1780 Gobara, Hiro-oka, Shiojiri, Nagano 399-0781, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Uchiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamichi
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Kinugawa
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: N
authors.initials: N
authors.lastname: Udagawa
authors.forename: H
authors.initials: H
authors.lastname: Miyazawa
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D018920:Coculture Techniques; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors; D008297:Male; D009000:Monocytes; D010010:Osteoclasts / Q000166:cytology* / Q000502:physiology; D053244:Osteoprotegerin / Q000096:biosynthesis; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D053245:RANK Ligand / Q000096:biosynthesis; D014091:Tooth Resorption / Q000378:metabolism
chemical_list: D018950:Lipopolysaccharide Receptors; D053244:Osteoprotegerin; D053245:RANK Ligand