title: Synergic stimulation of laminin and epidermal growth factor facilitates the myoblast growth through promoting migration.
abstract: The dynamic behaviors of human skeletal muscle myoblasts were investigated in the culture on a laminin-coated surface in the presence of 100 ng/ml epidermal growth factor (EGF) in medium. The coexistence of laminin and EGF caused the enhancement of myoblast migration, giving an average migration rate of 62.0 microm/h, which was 2.7 times that on a plain surface. This encouraged migration could be a driving force to separate the dividing cells from each other, accompanied by shortened disjunction time of daughter cells to complete cytokinesis. In addition, the synergic effect of laminin and EGF led to the promotion of myoblast growth with keeping a relatively high fraction of proliferative cells during the culture for 150 h, which is considered to arise from the reduced frequency of cell-cell contacts during cytokinesis and thereby suppressing the process towards myotube formation after cell division.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Shiplu Roy
authors.initials: SR
authors.lastname: Chowdhury
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Muneyuki
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Takezawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kino-Oka
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Saito
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D048749:Cytokinesis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000819:agonists / Q000494:pharmacology*; D006801:Humans; D007797:Laminin / Q000819:agonists / Q000494:pharmacology*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000502:physiology*
chemical_list: D007797:Laminin; D004815:Epidermal Growth Factor