Microarray-based determination of the lytic cascade of human herpesvirus 6B.
The lytic gene expression of several members of the human herpesvirus family has been profiled by using gene-expression microarrays; however, the lytic cascade of roseoloviruses has not been studied in similar depth. Based on the complete DNA genome sequences of human herpesvirus 6 variant A (HHV-6A) and variant B (HHV-6B), we constructed a cDNA microarray containing DNA probes to their predicted open reading frames, plus 914 human genes. Gene-expression profiling of HHV-6B strain Z29 in SupT1 cells over a 60 h time-course post-infection, together with kinetic classification of the HHV-6B genes in the presence of either cycloheximide or phosphonoacetic acid, allowed the placement of HHV-6B genes into defined kinetic classes. Eighty-nine HHV-6B genes were divided into four different expression kinetic classes: eight immediate-early, 44 early, 33 late and four biphasic. Clustering of genes with similar expression profiles implied a shared function, thus revealing possible roles of previously uncharacterized HHV-6B genes.
Tsao
Edward H
EH
Department of Infection, Division of Infection and Immunity, Royal Free and University College Medical School of UCL, London, UK.
Kellam
Paul
P
Virus Genomics Team, Wellcome Trust Sanger Institute, Cambridge, UK.|Department of Infection, Division of Infection and Immunity, Royal Free and University College Medical School of UCL, London, UK.
Sin
Cheryl S Y
CSY
Department of Infection, Division of Infection and Immunity, Royal Free and University College Medical School of UCL, London, UK.
Rasaiyaah
Jane
J
Department of Infection, Division of Infection and Immunity, Royal Free and University College Medical School of UCL, London, UK.
Griffiths
Paul D
PD
Department of Infection, Division of Infection and Immunity, Royal Free and University College Medical School of UCL, London, UK.
Clark
Duncan A
DA
Department of Infection, Division of Infection and Immunity, Royal Free and University College Medical School of UCL, London, UK.
D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D014779:Virus Replication
D000998:Antiviral Agents; D003513:Cycloheximide; D010746:Phosphonoacetic Acid