[Differentially expressed proteins between normal ovaries and ovarian cancer tissues screened by the protein chips].
OBJECTIVE
To explore the differentially expressed proteins between normal ovaries and ovarian cancer tissues using the protein chips.


METHODS
Tissues of 11 epithelial ovarian cancer (EOC) and 11 matched normal ovaries were labeled with cy3 and cy5 fluorescent dyes and then were hybridized with 512 monoclonal protein antibody chips. The internally normalized ratio (INR) was calculated according to the intensity of fluorescence of each protein spots. The value of INR > 2.0 or < 0.7 was considered as the cut-value to filtrate the differentially expressed proteins between tissues of EOC and normal ovaries.


RESULTS
Thirty one differentially expressed proteins were found between tissues of EOC and normal ovaries, in which 17 up-regulated and 14 down-regulated proteins involved in the transcription, proliferation, signal conduction, and apoptosis of cells.


CONCLUSIONS
Antibody chips can effectively screen the differentially expressed proteins between normal ovaries and ovarian cancer tissues.
Chen
Fei
F
Department of Obstetrics and Gynecology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Yang
Jia-Xin
JX
Wu
Ming
M
Shen
Keng
K
D000328:Adult; D000368:Aged; D000077216:Carcinoma, Ovarian Epithelial; D005260:Female; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D040081:Protein Array Analysis; D040901:Proteomics