title: [Preparation of the vaccine with inactivated Feline Panleukopenia Virus isolated from tiger and the preliminary application].
abstract: OBJECTIVE
  To prepare a vaccine with inactivated Feline Panleukopenia Virus (FPV) isolated from Siberian tigers and to evaluate its immunological effect.
  
  
  METHODS
  FPV-HLJ, an FPV strain previously isolated from Siberian tiger in our lab was used to inoculate cat kidney cell line F81 with dose 1/10(v/v) using the synchronizing inoculation method. Inoculated F81 cell line was cultured at 37 degrees C and collected when cytopathic effect was up to 75%. Viral suspension was inactivated by using formaldehyde for 24 h and inactive vaccine preapred by adding aluminium hydroxide gel as adjuvant to the suspension. The inactive vaccine was applied to 2-month-old kitten tigers of hypodermically after protection effect was proved in 2-month-old nonimmunized cats by using the same vaccination procedure.
  
  
  RESULTS
  Antibody level in vaccinated cats revealed an increasing trend that the valence of antibody reached 1:1024-2048 after three times of vaccination. All vaccinated cats survived challenges of virulent FPV virus. The valence of antibody reached 1:1024 in most vaccinated tigers 15 days after the third vaccination.
  
  
  CONCLUSIONS
  The results indicated that the inactivated vaccine can produce immunoprotection for tigers.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China. yuyali_1984@163.com
authors.forename: Yali
authors.initials: Y
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Hua
authors.forename: Ligang
authors.initials: L
authors.lastname: Wang
authors.forename: Yinan
authors.initials: Y
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
mesh_terms: D000818:Animals; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D005254:Feline Panleukopenia / Q000517:prevention & control*; D028301:Feline Panleukopenia Virus / Q000276:immunology*; D012800:Siberia; D046010:Tigers / Q000821:virology*; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines