Application of WET sequence for the detection of the ligand signals resonating close to water.
An efficient pulse sequence for observing the ligand signals resonating close to the water signal has been developed by incorporating the WET technique into the saturation transfer difference pulse sequence. Although several pulse sequences have been developed for observing a ligand binding with a protein receptor, the ligand signals resonating close to the water were undetectable owing to the interference of the huge water signal in the samples containing 95% (1)H(2)O. On the point of sample preparation, it is preferable to avoid the solvent exchange in the protein samples. In the proposed pulse sequence, a WET sequence is incorporated for the selective suppression of the water resonance. The efficient water suppression and the clear observation of the bound ligand signals close to the water have been demonstrated using the lysozyme-glucose complex.
Furihata
Kazuo
K
Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Shimotakahara
Sakurako
S
Shibusawa
Yoichi
Y
Tashiro
Mitsuru
M
D000465:Algorithms; D045424:Complex Mixtures / Q000737:chemistry; D005947:Glucose / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009113:Muramidase / Q000737:chemistry*; D011485:Protein Binding; D012815:Signal Processing, Computer-Assisted; D014867:Water / Q000737:chemistry*
D045424:Complex Mixtures; D008024:Ligands; D014867:Water; D009113:Muramidase; D005947:Glucose