[Regulation of c-fos gene expression in hepatic stellate cells by transforming growth factor beta].
OBJECTIVE
To investigate the effects of transforming growth factor beta (TGF ) on c-fos gene expression in hepatic stellate cells.


METHODS
Hepatic stellate cells (HSC-T6) were cultured in the medium containing different concentrations of TGF (0.2, 1, and 5 ng/ml), and cells were collected at different time points of incubation (8, 24, 48, and 72 h). The total RNA of the HSCs was isolated and c-fos gene expression level were measured by reverse transcription polymerase chain reaction.


RESULTS
c-fos gene expression levels of HSCs cultured in the presence of low (0.2 ng/ml), moderate (1 ng/ml) and high (5 ng/ml) concentrations of TGF for 8, 24, 48 and 72 h were significantly greater than those of control group. The c-fos gene expression levels of HSCs increased gradually with the increment of TGF concentration, and significant differences in c-fos gene expression were found between the 3TGF groups.


CONCLUSIONS
TGF strongly up-regulates c-fos gene expression in hepatic stellate cells.
Wang
Ai-Min
AM
Department of Gastroenterology, 252 Hospital of PLA, Baoding 071000, China. aiminwang62@sina.com
Ren
Chun-Gai
CG
Ma
Zhi-Jie
ZJ
Xiang
Ying
Y
Zhang
Zhi-Wei
ZW
Zhu
Zhi-Jian
ZJ
Wang
Bao-En
BE
D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos; D055166:Hepatic Stellate Cells / Q000187:drug effects*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016760:Proto-Oncogene Proteins c-fos; D016212:Transforming Growth Factor beta