title: Post-genomic insights into the plant polysaccharide degradation potential of  Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae.
abstract: The plant polysaccharide degradative potential of Aspergillus nidulans was analysed in detail and compared to that of Aspergillus niger and Aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. Manual verification indicated that 28.4% of the A. nidulans ORFs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method.While significant differences were found between the species in the numbers of ORFs assigned to the relevant CAZy families, no significant difference was observed in growth on polysaccharides. Growth differences were observed between the Aspergilli and Podospora anserina, which has a more different genomic potential for polysaccharide degradation, suggesting that large genomic differences are required to cause growth differences on polysaccharides. Differences were also detected between the Aspergilli in the presence of putative regulatory sequences in the promoters of the ORFs of this study and correlation of the presence of putative XlnR binding sites to induction by xylose was detected for A. niger. These data demonstrate differences at genome content, substrate specificity of the enzymes and gene regulation in these three Aspergilli, which likely reflect their individual adaptation to their natural biotope.
authors.affiliation: AFMB-UMR 6098 CNRS/Universités Aix- Marseille I and II, Case 932, 163 Av de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Coutinho
authors.forename: Mikael R
authors.initials: MR
authors.lastname: Andersen
authors.forename: Katarina
authors.initials: K
authors.lastname: Kolenova
authors.forename: Patricia A
authors.initials: PA
authors.lastname: vanKuyk
authors.forename: Isabelle
authors.initials: I
authors.lastname: Benoit
authors.forename: Birgit S
authors.initials: BS
authors.lastname: Gruben
authors.forename: Blanca
authors.initials: B
authors.lastname: Trejo-Aguilar
authors.forename: Hans
authors.initials: H
authors.lastname: Visser
authors.forename: Piet
authors.initials: P
authors.lastname: van Solingen
authors.forename: Tiina
authors.initials: T
authors.lastname: Pakula
authors.forename: Bernard
authors.initials: B
authors.lastname: Seiboth
authors.forename: Evy
authors.initials: E
authors.lastname: Battaglia
authors.forename: Guillermo
authors.initials: G
authors.lastname: Aguilar-Osorio
authors.forename: Jan F
authors.initials: JF
authors.lastname: de Jong
authors.forename: Robin A
authors.initials: RA
authors.lastname: Ohm
authors.forename: Mariana
authors.initials: M
authors.lastname: Aguilar
authors.forename: Bernard
authors.initials: B
authors.lastname: Henrissat
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
authors.forename: Henrik
authors.initials: H
authors.lastname: Stålbrand
authors.forename: Ronald P
authors.initials: RP
authors.lastname: de Vries
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001234:Aspergillus niger / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019295:Computational Biology; D004798:Enzymes / Q000235:genetics*; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D016678:Genome; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011134:Polysaccharides / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D011134:Polysaccharides