title: Nanomechanical in situ monitoring of proteolysis of peptide by Cathepsin B.
abstract: Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of peptides, which is well depicted by Langmuir kinetic model. This implies that nanomechanical biosensor enables the characterization of specific cellular protease such as its kinetics.
authors.affiliation: Research Institute for Engineering Technology, Korea University, Seoul, Republic of Korea. tykwon@korea.ac.kr
authors.forename: Taeyun
authors.initials: T
authors.lastname: Kwon
authors.forename: Jinsung
authors.initials: J
authors.lastname: Park
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Yoon
authors.forename: Sungsoo
authors.initials: S
authors.lastname: Na
authors.forename: Chang-Wan
authors.initials: CW
authors.lastname: Kim
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Kilho
authors.initials: K
authors.lastname: Eom
mesh_terms: D002401:Cathepsin B / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D036103:Nanotechnology; D010455:Peptides / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000378:metabolism
chemical_list: D010455:Peptides; D011092:Polyethylene Glycols; D002401:Cathepsin B