Depletion of tubulin polymerization promoting protein family member 3 suppresses HeLa cell proliferation.
Microtubules (MTs) play an important role in cell division, and their functions are regulated by a set of microtubule-associated proteins (MAPs). Tubulin polymerization promoting protein family member 3 (TPPP3), also known as p20, is a new member of the tubulin polymerization promoting protein (TPPP) family. Previous studies have demonstrated that TPPP3 specifically binds to MTs and positively regulates MTs assembly, which leads to significant ultrastructural alterations of the MTs network. However, the physiological function of TPPP3 is still largely unknown. In the present study, we showed that knockdown of endogenous TPPP3 by RNA interference (RNAi) suppressed cell proliferation and induced cell cycle arrest in HeLa cells. Furthermore, we showed that the depletion of TPPP3 caused mitotic abnormalities, such as the formation of multipolar spindles and chromosome segregation errors, which lead to apoptosis in HeLa cells. Our study suggested that TPPP3 played a crucial role in cell mitosis by regulating centrosomes amplification and/or spindles translocation processes.
Zhou
Wenbai
W
Department of Endocrinology and Metabolism, Huashan Hospital, Institute of Endocrinology and Diabetology, Department of Medicine, Shanghai Medical College, Fudan University, No.12 Wulumuqi Road, Shanghai, 200040, China.
Wang
Xuanchun
X
Li
Lianxi
L
Feng
Xiaocheng
X
Yang
Zhihong
Z
Zhang
Weiwei
W
Hu
Renming
R
D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D020090:Chromosome Segregation; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000502:physiology*; D008941:Spindle Apparatus / Q000378:metabolism
D009419:Nerve Tissue Proteins; C118136:TPPP protein, human