[Dracorhodin perchlorate inhibit high glucose-induced connective tissue growth factor formation in human mesangial cells].
OBJECTIVE
To study the effect of dracorhodin perchlorate on high glucose-induced connective tissue growth factor (CTGF) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).


METHODS
The HMC were divided into low glucose group (LG group, 5.5 mmol x L(-1) D-glucose), high glucose group (HG Group 25 mmol x L(-1) D-glucose), each group was located with dracorhodin perchlorate (7. 5 micromol x L(-1)) as comparison, and was examined at 24, 48 h. The expression of CTGF mRNA was assessed by semi quantatiue RT-PCR, and the expression of CTGF protein was assessed by Western blot.


RESULTS
Compared to HG group, the expression of CTGF mRNA and protein were reduced in LG group after stimulating 24, 48 hours (P < 0.01). Compared to HG group, the expression of CTGF mRNA and protein were reduced in HG group by added with dracorhodin perchlorate after stimulating 24, 48 hours (P < 0.01).


CONCLUSIONS
Dracorhodin perchlorate can inhibit high glucose-induced connective tissue growth factor expression in human mesangial cells, and this may be its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).
Wang
Yonghui
Y
Department of Integrated Chinese Medicine and Western Medicine, Union Hospital, Tongfi Medical College, Huazhong Science and Technology University, Wuhan 430022, China.
Wang
Quansheng
Q
Liu
Jianguo
J
Zhang
Lixiao
L
Zhang
Bing
B
D001578:Benzopyrans / Q000494:pharmacology*; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D001578:Benzopyrans; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor; C060327:dracorhodin; D005947:Glucose