Effects of epigallocatechin gallate on osteogenic capability of human mesenchymal stem cells after suspension in phosphate-buffered saline.
Administration of culture-expanded mesenchymal stem cells (MSCs) has been sought for regeneration of various damaged tissues/organs in clinical situations. Liquid suspensions of MSCs have either been directly injected into organs or generally infused. In this study, we focused on the viability and differentiation of MSCs after suspension in phosphate-buffered saline. When the MSCs were treated with epigallocatechin gallate, which is purified from green tea catechin, the MSCs showed high viability as well as osteogenic differentiation capability even while suspended in phosphate-buffered saline for 4 days at 4 degrees C. In contrast, nontreated MSCs showed low viability and showed hardly any differentiation. The rate of proliferation of the treated MSCs was much higher than that of the nontreated MSCs. These results indicate the usefulness of epigallocatechin gallate treatment for fabrication of ready-to-use cellular products of MSC suspension.
Kashiwa
Kaori
K
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki, Japan.
Kotobuki
Noriko
N
Tadokoro
Mika
M
Matsumura
Kazuaki
K
Hyon
Suong-Hyu
SH
Yoshiya
Shinichi
S
Ohgushi
Hajime
H
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D002021:Buffers; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D010710:Phosphates / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
D000975:Antioxidants; D002021:Buffers; D010710:Phosphates; D012965:Sodium Chloride; D002392:Catechin; C045651:epigallocatechin gallate