Performance of the RealStar Chikungunya virus real-time reverse transcription-PCR kit.
A novel commercial Chikungunya virus real-time reverse transcription-PCR (RT-PCR) kit was evaluated on a comprehensive panel of original patient samples. The assay was 100% sensitive and specific in comparison to a published real-time RT-PCR. Viral loads from both assays were highly correlated. The kit proved to be suitable for routine use in patient care.
Panning
Marcus
M
Institute of Virology, University of Bonn Medical Centre, Sigmund Freud-Str. 25, 53127 Bonn, Germany.
Hess
Markus
M
Fischer
Waldemar
W
Grywna
Klaus
K
Pfeffer
Martin
M
Drosten
Christian
C
D018354:Alphavirus Infections / Q000175:diagnosis*; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019562:Viral Load
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic