Follicle development and luteal cell morphology altered by phosphodiesterase-5 inhibitor.
Vardenafil citrate is a potent vasodilator used in the treatment of patients with erectile dysfunction. Its mechanism of action is based on the selective inhibition of phosphodiesterase-5 (PDE5), specific to guanosine 3',5'-cyclic monophosphate (cGMP). Recently, chronic treatment with Vardenafil has been successfully used in cases of pulmonary hypertension and, despite being used in high doses for long periods, little is known about its effects on other systems. In the present study, female mice were treated daily with 5 mg/kg Vardenafil for 4 weeks, after which the ovaries were collected for morphological analyses and sera were collected for biochemical assays. This study found that treatment with Vardenafil decreased HDL serum levels and the number of antral follicles as well as induced lesser lipid content in luteal cells, suggesting that high levels of cGMP may affect follicle development.
Donato
Mariana Aragão Matos
MA
Laboratório de Ultraestrutura do Centro de Pesquisas Aggeu Magalhães (FIOCRUZ), Recife, Brazil.
Saraiva
Karina Lidianne Alcântara
KL
Soares e Silva
Amanda Karolina
AK
Wanderley
Maria Inês
MI
Peixoto
Christina Alves
CA
D000818:Animals; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006651:Histocytochemistry; D007093:Imidazoles / Q000008:administration & dosage / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000032:analysis; D008184:Luteal Cells / Q000187:drug effects* / Q000648:ultrastructure*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development; D058986:Phosphodiesterase 5 Inhibitors; D010879:Piperazines / Q000008:administration & dosage / Q000494:pharmacology*; D044967:Serum / Q000737:chemistry; D013450:Sulfones / Q000008:administration & dosage / Q000494:pharmacology; D014227:Triazines / Q000008:administration & dosage / Q000494:pharmacology; D000069058:Vardenafil Dihydrochloride
D004791:Enzyme Inhibitors; D007093:Imidazoles; D008075:Lipoproteins, HDL; D058986:Phosphodiesterase 5 Inhibitors; D010879:Piperazines; D013450:Sulfones; D014227:Triazines; D000069058:Vardenafil Dihydrochloride