title: Solid lipid nanoparticles bearing oxybenzone: in-vitro and in-vivo evaluation.
abstract: In the present project, Solid Lipid Nanoparticles (SLNs) bearing oxybenzone were prepared by ethanol injection method to improve its effectiveness as sunscreen. SLNs were characterized for particle size,polydispersity index, zeta potential and surface morphology. The optimized SLNs bearing oxybenzone were incorporated into water-removable cream base and compared with SLNs unloaded water-removable cream base for in vitro and in vivo parameters. Cream base formulation containing SLNs (Csd) with 5% oxybenzone showed slow drug release and better sun protecting factor (more than 25) compared to cream base containing 5% oxybenzone. Confocal Laser Scanning Microscopy was used to visualize the distribution of developed formulations in skin. CLSM indicated prolonged retention of SLNs in the stratum corneum as compared to plain cream base. These studies revealed that the cream base bearing SLNs exhibited good skin retention as well as enhanced sun protection effect compared to cream base.
authors.affiliation: Pharmaceutics Research Projects Laboratory, Department of Pharmaceutical Sciences, Dr Hari Singh Gour Vishwavidyalaya, Sagar, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Gulbake
authors.forename: Aviral
authors.initials: A
authors.lastname: Jain
authors.forename: Piush
authors.initials: P
authors.lastname: Khare
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Jain
mesh_terms: D001577:Benzophenones / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D004890:Erythema / Q000473:pathology / Q000517:prevention & control; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D023422:Skin Irritancy Tests; D013473:Sunscreening Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D001577:Benzophenones; D008055:Lipids; D013473:Sunscreening Agents; C005290:oxybenzone