title: Sigma-2 receptor expression in bovine papillomavirus-associated urinary bladder tumours.
abstract: The expression of sigma-2 receptors was investigated in nine urothelial tumours of the urinary bladder of cattle. Each tumour was associated with the presence of DNA of bovine papillomavirus type-2 (BPV-2) and expression of the E5 viral oncoprotein. Five tumours were classified as low-grade carcinoma on the basis of morphological criteria and calculation of mean nuclear area (MNA) and mean nuclear perimeter (MNP). Four tumours were classified as high-grade carcinoma. Sigma-2 receptors were overexpressed in both types of carcinoma. In control normal bovine bladder tissue the density of receptors (expressed as the B(max)) was 0.37 pmol/mg of protein. Low-grade carcinomas had a mean B(max) of 1.37+/-0.32 pmol/mg of protein (range 1.03-1.86) and in high-grade carcinomas the mean B(max) was 10.9+/-2.8 pmol/mg of protein (range 8.2-14). The difference in B(max) between low- and high-grade carcinomas was statistically significant (P=0.0001).
authors.affiliation: Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Naples University Federico II, 80137 Naples, Italy. sante.roperto@unina.it
authors.forename: S
authors.initials: S
authors.lastname: Roperto
authors.forename: N A
authors.initials: NA
authors.lastname: Colabufo
authors.forename: C
authors.initials: C
authors.lastname: Inglese
authors.forename: C
authors.initials: C
authors.lastname: Urraro
authors.forename: R
authors.initials: R
authors.lastname: Brun
authors.forename: E
authors.initials: E
authors.lastname: Mezza
authors.forename: S
authors.initials: S
authors.lastname: Staibano
authors.forename: C
authors.initials: C
authors.lastname: Raso
authors.forename: P
authors.initials: P
authors.lastname: Maiolino
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.forename: E
authors.initials: E
authors.lastname: Palma
authors.forename: F
authors.initials: F
authors.lastname: Roperto
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000378:metabolism* / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D047468:Immunoprecipitation; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism / Q000628:therapy* / Q000662:veterinary; D016133:Polymerase Chain Reaction; D017480:Receptors, sigma / Q000096:biosynthesis*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000662:veterinary* / Q000821:virology
chemical_list: D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D017480:Receptors, sigma; C054304:oncogene protein E5, Bovine papillomavirus type 1; C106107:sigma-2 receptor