Genotyping of strawberry (Fragaria x ananassa Duch.) cultivars by DNA markers: interlaboratory study.
Fourteen Japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (CAPS) markers for discrimination of strawberry (Fragaria x ananassa Duch.) cultivars. Both the sensitivity and specificity rate of 12 markers were 100%, those of another 12 were >95%, and those of 1 were >90%. These results indicate that the method of genotyping by the CAPS markers was highly reproducible and could provide a useful basis for practical identification of strawberry cultivars. This is the first report of the statistical validation of crop genotyping by DNA markers.
Kunihisa
Miyuki
M
National Institute of Vegetable and Tea Science, Kannondai 3-1-1, Tsukuba, Ibaraki 305-8666, Japan. miyuky@affrc.go.jp
Ueda
Hiroshi
H
Fukino
Nobuko
N
Matsumoto
Satoru
S
D018556:Crops, Agricultural / Q000235:genetics*; D031985:Fragaria / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D012680:Sensitivity and Specificity
D005819:Genetic Markers