Exploiting and antagonizing microRNA regulation for therapeutic and experimental applications.
New technologies are emerging that utilize artificial microRNA (miRNA) target sites to exploit or inhibit endogenous miRNA regulation. This approach has been used to improve cell-specific targeting for gene and stem cell therapy studies and for animal transgenics, and also to reduce the toxicity of oncolytic viruses and to attenuate viral vaccines. Artificial targets have also been used to sponge or decoy miRNAs as a way to study their functions. This article considers the benefits of this approach and design considerations for future studies.
Brown
Brian D
BD
Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, 1425 Madison Avenue, BOX 1498, New York, New York 10029, USA. brian.brown@mssm.edu
Naldini
Luigi
L
D000818:Animals; D015316:Genetic Therapy; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors*; D009369:Neoplasms / Q000235:genetics / Q000628:therapy*
D035683:MicroRNAs