title: Reproducibility of 3.0 Tesla magnetic resonance spectroscopy for measuring hepatic fat content.
abstract: OBJECTIVE
  To investigate reproducibility of proton magnetic resonance spectroscopy ((1)H-MRS) to measure hepatic triglyceride content (HTGC).
  
  
  METHODS
  In 24 subjects, HTGC was evaluated using (1)H-MRS at 3.0 Tesla. We studied "between-weeks" reproducibility and reproducibility of (1)H-MRS in subjects with fatty liver. We also studied within liver variability and within day reproducibility. Reproducibility was assessed by coefficient of variation (CV), repeatability coefficient (RC), and intraclass correlation coefficient (ICC).
  
  
  RESULTS
  The CV of between weeks reproducibility was 9.5%, with a RC of 1.3% HTGC (ICC 0.998). The CV in fatty livers was 4.1%, with a RC of 1.3% HTGC (ICC 0.997). Within day CV was 4.5%, with a RC of 0.4% HTGC (ICC 0.999). CV for within liver variability was 14.5%.
  
  
  CONCLUSIONS
  Reproducibility of (1)H-MRS to measure HTGC for "between-weeks" measurements and in fatty livers is high, which is important for follow-up studies. Within liver variability displays a larger variation, meaning that liver fat is not equally distributed and during consecutive measurements the same voxel position should be used.
authors.affiliation: Department of Radiology, Academic Medical Center at the University of Amsterdam, Amsterdam, the Netherlands. j.r.vanwerven@amc.uva.nl
authors.forename: Jochem R
authors.initials: JR
authors.lastname: van Werven
authors.forename: Johannes M
authors.initials: JM
authors.lastname: Hoogduin
authors.forename: Aart J
authors.initials: AJ
authors.lastname: Nederveen
authors.forename: Andre A
authors.initials: AA
authors.lastname: van Vliet
authors.forename: Ewa
authors.initials: E
authors.lastname: Wajs
authors.forename: Petra
authors.initials: P
authors.lastname: Vandenberk
authors.forename: Erik S G
authors.initials: ES
authors.lastname: Stroes
authors.forename: Jaap
authors.initials: J
authors.lastname: Stoker
mesh_terms: D000328:Adult; D000368:Aged; D005234:Fatty Liver / Q000473:pathology*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications; D010865:Pilot Projects; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric