[Analysis of genetic diversity and biological functional site of HIV-1 gp120 from a patient with AIDS dementia complex].
To explore the relationship between the genetic diversity and biological functional site of human immunodeficiency virus HIV-1 gp120 and the pathogenesis of AIDS dementia complex (ADC), the full length sequences of gp120 gene was amplified with PCR from genomic DNA which was extracted from lymphoid and different brain department (periaortic lymphoid, temporal gray/white matter junction, periventricular tissue, choroids plexus, occipital white matter and occipital gray/white matter junction.) of a patient who died of ADC. PCR products were cloned into the pGEM-T vector and positive clones were sequenced. The analysis of neighbor-joining tree, N-glycosylation sites, values of ds/dn, and loop were then all performed. The samples were all identified as HIV-1 B and genetic variation existed in HIV-1 gp120 isolated from different tissues. Compared with standard HIV-1B gp120, biological functional sites of HIV-1 gp120 isolated from the patient changed to some extent. In addition, there were differences in some biological functional sites of HIV-1 gp120 between lymphoid and brain. Therefore, genetic diversity and alterations of some biological functional sites of HIV-1 gp120 might be associated with the pathogenesis of ADC.
Yan
Yu-Fen
YF
Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.
Zhao
Li
L
Wang
Zhi-Yu
ZY
McGrath
Michael S
MS
Hou
Xiao-Yu
XY
Zheng
Xiao-Min
XM
Song
Yan-Yan
YY
Yao-Ping
Wen
Hong-Ling
HL
Wang
Gui-Ting
GT
Xu
Hong-Zhi
HZ
D015526:AIDS Dementia Complex / Q000821:virology*; D000595:Amino Acid Sequence; D014644:Genetic Variation / Q000235:genetics*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D015699:HIV Envelope Protein gp120