Expression of nerve growth factor immunoreactivity and messenger RNA in ischemic urinary bladder.
OBJECTIVE
The bladder contractile dysfunction resulting from acute ischemia may be attributed to nerve growth factor (NGF) overexpression. This study was conducted to evaluate the acute and mid-term effects of bladder ischemia on the temporal expression of NGF immunoreactivity and mRNA.


METHODS
Bladder ischemia was induced by ligation of bilateral vesical arteries in female rats. We examined the NGF content of bladder detrusor muscle at 1 day, 1 week and 4 weeks after artery ligation. Immunoreactivity of NGF was studied by immunofluorescent staining and Western blot. The NGF mRNA was analyzed by real-time polymerase chain reaction.


RESULTS
The immunofluorescence of NGF at 1 week and 4 weeks was significantly reduced when compared to sham-operated group (P < 0.05). This decreased tendency was also found in Western blot test. An increased expression of NGF mRNA was noted at 1 day, 1 week and 4 weeks, but had no significant change when compared to sham-operated group (P > 0.05).


CONCLUSIONS
Our study showed bilateral vesical artery ligation may cause damage of detrusor muscle and there is decreased NGF immunofluorescence and elevated NGF mRNA in bladder suggesting an expression disparity following ischemia.
Liang
Ching-Chung
CC
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Linkou Medical Center, Kweishan, Taoyuan, Taiwan.
Tseng
Ling-Hong
LH
Ko
Yu-Shien
YS
Lee
Tsong-Hai
TH
D000818:Animals; D005260:Female; D007511:Ischemia / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000235:genetics* / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D001743:Urinary Bladder / Q000098:blood supply*
D012333:RNA, Messenger; D020932:Nerve Growth Factor