title: Detection of apoptosis in mammalian development.
abstract: Mammalian development is dependent on an intricate orchestration of cell proliferation and death. Deregulation in the levels, localization, and type of cell death can lead to disease and even death of the developing embryo. The mechanisms involved in such deregulation are many; alterations and or manipulations of these can aid in the detection, prevention and possible treatments of any effects this de-regulation may have. Here we describe how cell death can be detected during mammalian development, using diverse staining and microscopy methods, while taking advantage of the advancements in cell death mechanisms, derived from biochemical and teratological studies in the field.
authors.affiliation: Medarex Inc, Bloomsbury, NY, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Carlos
authors.initials: C
authors.lastname: Penaloza
authors.forename: Yixia
authors.initials: Y
authors.lastname: Ye
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lockshin
authors.forename: Zahra
authors.initials: Z
authors.lastname: Zakeri
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000032:analysis / Q000378:metabolism; D051360:Cyclin-Dependent Kinase 5 / Q000032:analysis / Q000378:metabolism; D003584:Cytological Techniques; D053938:DNA Fragmentation; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008247:Lysosomes / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D010587:Phagocytosis; D010718:Phosphatidylserines / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy; D016707:Tissue Fixation
chemical_list: D010718:Phosphatidylserines; D051360:Cyclin-Dependent Kinase 5; D053148:Caspase 3