[Effects of natural plant ginkgolic acids on the apoptosis of human Hep-2 cancer cells].
OBJECTIVE
To investigate the anti-cancer effects and mechanism of natural plant ginkgolic acids (GAs) on Human Hep-2 cancer cells.


METHODS
Hep-2 cancer cells were treated with different concentration of GAs for different times. The proliferation of Hep-2 cancer cells were detected by MTT colorimetric assay. Biochemistry characteristics of cell apoptosis was analyzed by DNA agarose electrophoresis and FCM.


RESULTS
The proliferation of Hep-2 cancer cells were inhibited significantly by GAs in a dose and time-dependent manner. Typical DNA ladder bands of Hep-2 cell DNA treated with GAs were observed on agarose electrophoresis gels. Increased apoptosis of Hep-2 cells treated with GAs was also noticed with FCM analysis.


CONCLUSIONS
Ginkgolic acids show an anti-tumor activity through inhibition the growth and inducing apoptosis of tumor cell in vitro.
Zhou
Chen-chen
CC
State Key Laboratory of Oral Disease, Sichuan University, Chengdu 610041, China.
Du
Wei
W
Wen
Zong
Z
Li
Ji-yao
JY
Zhang
Ping
P
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D012459:Salicylates / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D012459:Salicylates; C112485:ginkgolic acid