[The pattern of centriole immunostaining for tyrosinated or acetylated alpha-tubulin changes during mitosis in 3T3 (A31) cells but not in SV40-3T3 cells].
Comparative electron microscopic analysis of the patterns of centriolar and cytoplasmic microtubules immunostaining for acetylated or tyrosinated alpha-tubulin was performed during the cell cycles of the mouse diploid 3T3 (A31) and virus-transformed heteroploid SV40-3T3 cell lines. It was shown that the pattern of centriole immunostaining changed during mitosis in control 3T3 (A31) cells, but not in tumorigenic SV40-3T3 cells. A sharp change in the pattern of centriole immunostaining was observed in those cell cycle periods when the protein organization of centrosome underwent changes during interphase/mitosis or mitosis/interphase transitions due to the rearrangement of microtubule system. A high level of centriole immunostaining for tyrosinated and acetylated alpha-tubulin was observed at all cell cycle stages with the exception of entering mitosis for the former and the end of mitosis for the latter.
Balashova
E E
EE
Lokhov
P G
PG
Bystrevskaia
V B
VB
D000107:Acetylation; D000818:Animals; D041702:BALB 3T3 Cells; D002472:Cell Transformation, Viral; D002502:Centrioles / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008938:Mitosis; D013539:Simian virus 40; D014404:Tubulin / Q000378:metabolism*
D014404:Tubulin; C039520:tyrosine-tubulin