title: The role of translationally controlled tumor protein in tumor growth and metastasis of colon adenocarcinoma cells.
abstract: Translationally controlled tumor protein (TCTP) plays a major role in a broad array of biological processes. However, the TCTP-related biological process and interactive proteins still remain poorly characterized. In the present study, we found that knockdown of TCTP inhibited proliferation, migration, and invasion activities of LoVo cells in vitro and in vivo. The whole-cell proteomes were compared by 2D gel electrophoresis before and after knockdown of TCTP. Alterations in 27 proteins were detected and their identities were revealed by mass spectrometry analysis. Components of Ubiquitin-Proteasome System, proteins involved in the cytoskeleton biosynthesis and tumor metastasis were found to be changed upon TCTP removal. These results imply that TCTP might play at least a partial role in colon adenocarcinoma progression.
authors.affiliation: The Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Geng
authors.forename: Weiwen
authors.initials: W
authors.lastname: Xu
authors.forename: Yingsong
authors.initials: Y
authors.lastname: Wu
authors.forename: Fuli
authors.initials: F
authors.lastname: He
authors.forename: Wen
authors.initials: W
authors.lastname: Shu
authors.forename: Maoliang
authors.initials: M
authors.lastname: Huang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Du
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002448:Cell Adhesion / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D014176:Protein Biosynthesis; D040901:Proteomics / Q000379:methods; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000090324:Tumor Protein, Translationally-Controlled 1; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; C000715647:TPT1 protein, human; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1