Dysplastic cells in cytological cervical samples show a high incidence of chromosomal abnormalities.
Chromosomal abnormalities are frequent in most cervical cancers. Amplifications of both the 3q26 (TERC) and 8q24 (MYC) loci have been shown to be prevalent in both high-grade lesions and invasive cervical carcinoma. Most of these studies have looked at either the histological sample or at the entire cytological population of cells. We have developed a Papanicolaou (Pap) destaining method that allows for the accurate analysis of individual cells that were previously identified by cytopathology as dysplastic. The application of fluorescence in situ hybridization (FISH) was then implemented to determine the chromosomal status of the dysplastic cells in the samples and correlate the two events. Chromosomal abnormality is over a thousand times more frequent in dysplastic cells compared with their morphologically normal counterparts.
Song
Minghao
M
Abbott Molecular, Inc., Des Plaines, Illinois 60018, USA.
Ruth
Adam
A
Policht
Frank A
FA
Bubendorf
Lukas
L
Feichter
Georg
G
Kipp
Benjamin R
BR
Halling
Kevin C
KC
Sokolova
Irina A
IA
D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002584:Cervix Uteri / Q000473:pathology*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D065006:Papanicolaou Test; D014626:Vaginal Smears / Q000379:methods*