title: [Development of single base extension-tags microarray for the detection of food-borne pathogens].
abstract: We developed single base extension-tags (SBE-tags) microarray to detect eight common food-borne pathogens, including Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella, Enterobacter sakazaki, Shigella, Escherichia coli O157:H7 and Campylobacter jejuni. With specific PCR primers identified and integrated for eight food-borne pathogens, target sequences were amplified and purified as template DNA of single base extension-tags reaction. The products were hybridized to microarrays and scanned for fluorescence intensity. The experiment showed a specific and simultaneous detection of eight food-borne pathogens. The system limits is 0.1 pg for a genomic DNA and 5x10(2) CFU/mL for Salmonella typhimurium cultures. The single base extension-tags assay can be used to detect food-borne pathogens rapidly and accurately with a high sensitivity, and provide an efficient way for diagnosis and control of disease caused by food-borne pathogens.
authors.affiliation: Department of Food Science and Bor Luh Food Safety Center, School ofAgriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Changyong
authors.initials: C
authors.lastname: Lu
authors.forename: Chunlei
authors.initials: C
authors.lastname: Shi
authors.forename: Chunxiu
authors.initials: C
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xianming
authors.initials: X
authors.lastname: Shi
mesh_terms: D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification