Transport of live yeast and zebrafish embryo on a droplet digital microfluidic platform.
We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays.
Son
Sang Uk
SU
Department of Chemistry and Biochemistry and California NanoSystems Institute, University of California Los Angeles, CA 90095-1569, USA.
Garrell
Robin L
RL
D000818:Animals; D046149:Embryo Culture Techniques; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009038:Motion; D011309:Preservation, Biological / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D015027:Zebrafish / Q000196:embryology*