title: LH/hCGR gene expression in human cumulus cells is linked to the expression of the extracellular matrix modifying gene TNFAIP6 and to serum estradiol levels on day of hCG administration.
abstract: BACKGROUND
  Recent studies suggest a role for luteinizing hormone and human chorionic gonadotrophin receptor (LH/hCGR) signalling in the regulation of the oocyte-cumulus oophorus cell interplay. The present study aimed at assessing the LH/hCGR gene expression in cumulus cells (CCs) surrounding oocytes in patients undergoing controlled ovarian hyperstimulation (COS) before ICSI and to relate the LH/hCGR expression to other COS quality parameters.
  
  
  METHODS
  CCs from single oocytes of normal responder patients were analysed by DNA microarrays. Concomitantly, estradiol levels on the day of hCG administration, CC morphology, total collected oocyte and metaphase II oocyte number were assessed in relation to LH/hCGR gene expression in CC.
  
  
  RESULTS
  The transcriptome analysis of CC indicated a variable expression of LH/hCGR among the patients and intra-patients. LH/hCGR mRNA expression was negatively correlated with serum estradiol level on the day of hCG administration. Eighty-five genes were significantly modulated between CCs from patients with a high and a low LH/hCGR expression. These genes are involved principally in steroid metabolism and in the ovulation process and include TNFAIP6, a gene expressed during CC-oocyte complex (COC) expansion. There were no significant differences in LH/hCGR gene expression profile between COS protocols.
  
  
  CONCLUSIONS
  LH/hCGR is expressed in CC under COS conditions. LH/hCGR expression level is associated with TNFAIP6 gene expression and negatively correlated with serum estradiol level on the day of hCG administration.
authors.affiliation: Institut de Recherche en Biothérapie, CHU Montpellier, Université Montpellier I, Hôpital Saint-Eloi, Montpellier F-34000, France.
authors.forename: D
authors.initials: D
authors.lastname: Haouzi
authors.forename: S
authors.initials: S
authors.lastname: Assou
authors.forename: K
authors.initials: K
authors.lastname: Mahmoud
authors.forename: B
authors.initials: B
authors.lastname: Hedon
authors.forename: J
authors.initials: J
authors.lastname: De Vos
authors.forename: D
authors.initials: D
authors.lastname: Dewailly
authors.forename: S
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D016000:Cluster Analysis; D054885:Cumulus Cells / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000097:blood*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007986:Luteinizing Hormone / Q000235:genetics / Q000378:metabolism*; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D015815:Cell Adhesion Molecules; D006063:Chorionic Gonadotropin; D011974:Receptors, LH; C072273:TNFAIP6 protein, human; D004958:Estradiol; D007986:Luteinizing Hormone