Electrogenerated chemiluminescence ethanol biosensor based on alcohol dehydrogenase functionalized Ru(bpy)3(2+) doped silica nanoparticles.
An ethanol biosensor, based on the electrogenerated chemiluminescence of Ru(bpy)(3)(2+)-doped silica nanoparticles (RuSiNPs), was investigated in this study. The biosensor was a modified glassy carbon electrode, where alcohol dehydrogenase was crosslinked to RuSiNPs, and then immobilized on the electrode surface using chitosan. The results indicated that the biosensor exhibited excellent performance during ethanol determination with a wide linear range (10(-7) to 10(-2) M), low detection limit (5.0x10(-8) M) and good stability.
Jia
Tian-tian
TT
Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Cai
Zhi-min
ZM
Chen
Xiao-mei
XM
Lin
Zhi-jie
ZJ
Huang
Xiao-li
XL
Chen
Xi
X
Chen
Guo-nan
GN
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D056831:Coordination Complexes; D004563:Electrochemistry / Q000379:methods; D000431:Ethanol / Q000032:analysis*; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D000426:Alcohol Dehydrogenase; D056831:Coordination Complexes; D000431:Ethanol; D012822:Silicon Dioxide; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds