title: Proliferation of mouse embryonic stem cell progeny and the spontaneous contractile activity of cardiomyocytes are affected by microtopography.
abstract: The niche in which stem cells reside and differentiate is a complex physicochemical microenvironment that regulates cell function. The role played by three-dimensional physical contours was studied on cell progeny derived from mouse embryonic stem cells using microtopographies created on PDMS (poly-dimethyl-siloxane) membranes. While markers of differentiation were not affected, the proliferation of heterogeneous mouse embryonic stem cell-derived progeny was attenuated by 15 microm-, but not 5 microm-high microprojections. This reduction was reversed by Rho kinase and myosin light chain kinase inhibition, which diminishes the tension generating ability of stress fibers. Purified cardiomyocytes derived from embryonic stem cells also showed significant blunting of proliferation and increased beating rates compared with cells grown on flat substrates. Thus, proliferation of stem cell-derived progeny appears to be regulated by microtopography through tension-generation of contractility in the third-dimension. These results emphasize the importance of topographic cues in the modulation of stem cell progeny behavior.
authors.affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois 60612-7342, USA.
authors.forename: Jesse K
authors.initials: JK
authors.lastname: Biehl
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamanaka
authors.forename: Tejal A
authors.initials: TA
authors.lastname: Desai
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Boheler
authors.forename: Brenda
authors.initials: B
authors.lastname: Russell
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D022081:Cell Surface Extensions / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D018613:Microscopy, Confocal; D009200:Myocardial Contraction / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors; D013499:Surface Properties; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors
chemical_list: D054460:rho-Associated Kinases; D009219:Myosin-Light-Chain Kinase