Experimental morphological and functional study of gentamicin cochleotoxicity using the regular dose given to neonates.
BACKGROUND
gentamicin is an antibiotic that acts in Gram-negative bacilli infections, having as a side effect ototoxicity. Ototoxicity is an iatrogenic disturb provoked by drugs that modify the internal ear, affecting the cochlear and/or vestibular system and causing alterations in two important functions: equilibrium and audition. The main pediatric groups that receive aminoglicosides antibiotics are newborns who present serious infections in Neonate intensive care units.


OBJECTIVE
to verify the occurrence of external ciliary cells (ECC) caused by gentamicin with single dose schemas of 4 mg/kg/day and 2,5mg/kg/day every 12 hours, through a morphological - scanning electronic microscopy (SEM) and functional - distortion product otoacoustic emissions (DPOAE) experimental study.


METHODS
26 albino guinea pigs were evaluated through DPOAE pre and post gentamicin treatment. The guinea pigs were sacrificed in the programmed time after the intramuscular administration of the drugs for anatomic analysis using MEV.


RESULTS
the evaluation of the functional state of the ECC indicated preservation of the DPOAE in all of the guinea pigs. The SEM results, after being photographed were analyzed in terms of the number of the ECC in the cochlear basal turn in a specific photographic field.


CONCLUSIONS
lesions or alterations in the functioning of the ECC of albino guinea-pigs after the use of 4 mg/Kg/day and 2,5mg/Kg/day every 12 hours for a period 10 and 14 days were not observed.
Baggio
Carla Luiza
CL
Hospital das Forças Armadas de Brasília. cabaggio@ig.com.br
Silveira
Aron Ferreira
AF
Hyppolito
Miguel Angelo
MA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005839:Gentamicins / Q000009:adverse effects*; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects* / Q000502:physiology
D000900:Anti-Bacterial Agents; D005839:Gentamicins