Association of (pro)renin receptor mRNA expression with angiotensin-converting enzyme mRNA expression in human artery.
OBJECTIVE
A significant role of (pro)renin receptor in the pathogenesis of end-organ damage has been suggested only in animal studies. This study was conducted to examine the mRNA expression of (pro)renin receptor in human artery.


METHODS
In 141 kidney failure patients, the mRNA was harvested from arterial fragments obtained during surgery constructing an arteriovenous access for hemodialysis therapy, and expression levels of (pro)renin receptor and other components of the renin-angiotensin system were determined.


RESULTS
Arterial (pro)renin receptor expression was similar in diabetic and non-diabetic patients, although plasma prorenin levels were significantly higher in the diabetic patients than in the non-diabetic patients. The arterial (pro)renin receptor mRNA levels of the hypertensive patients, who had not been treated with either angiotensin-converting enzyme (ACE) inhibitors or angiotensin II type 1 receptor blockers, were significantly lower than those of the patients who had been treated with either drug. Multiple regression analyses showed a significant association with a large coefficient between the arterial mRNA level of the (pro)renin receptor and the arterial mRNA level of ACE; this significant association disappeared in patients who had been treated with either drug.


CONCLUSIONS
(Pro)renin receptor may contribute to the generation of arterial angiotensin II in kidney failure patients.
Takemitsu
Tomoko
T
Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Ichihara
Atsuhiro
A
Kaneshiro
Yuki
Y
Sakoda
Mariyo
M
Kurauchi-Mito
Asako
A
Narita
Tatsuya
T
Kinouchi
Kenichiro
K
Yamashita
Norimasa
N
Itoh
Hiroshi
H
D000368:Aged; D000450:Aldosterone / Q000097:blood; D047228:Angiotensin II Type 1 Receptor Blockers / Q000627:therapeutic use; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000627:therapeutic use; D001158:Arteries / Q000502:physiology*; D001166:Arteriovenous Shunt, Surgical; D003928:Diabetic Nephropathies / Q000235:genetics / Q000503:physiopathology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D006977:Hypertension, Renal / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*; D012044:Regression Analysis; D012083:Renin / Q000097:blood; D000093342:Prorenin Receptor
D047228:Angiotensin II Type 1 Receptor Blockers; D000806:Angiotensin-Converting Enzyme Inhibitors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D000450:Aldosterone; D007703:Peptidyl-Dipeptidase A; D012083:Renin; D000093342:Prorenin Receptor