NMR assignments of the binary hvDHFR1:folate complex.
A better understanding of how salt affects enzyme activity can be gained via NMR studies of binary hvDHFR1:folate complex. Chemical shift assignments of the 17.9 kDa enzyme with bound substrate prepare the way for ongoing research of the effects of salt on enzyme flexibility through relaxation studies.
Boroujerdi
Arezue F B
AF
Department of Chemistry, Mississippi State University, Box 9573, Mississippi State, MS 39762, USA.
Binbuga
Bulent
B
Young
John K
JK
D005492:Folic Acid / Q000737:chemistry; D019622:Haloferax volcanii / Q000201:enzymology* / Q000235:genetics; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D054712:Salinity; D013379:Substrate Specificity; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry* / Q000235:genetics
D011994:Recombinant Proteins; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase