Detection of rotavirus RNA and antigens in serum and cerebrospinal fluid samples from diarrheic children with seizures.
Group A rotavirus (GARV) genes (the VP7 and NSP3 genes) in acute-phase cerebrospinal fluid (CSF), sera and stool samples from 6 children with convulsions accompanied by GARV gastroenteritis were investigated by reverse transcription-polymerase chain reaction (RT-PCR). When the VP7 gene was amplified from the samples, the G genotype (G type) of GARV was determined by RT-PCR. GARV genes were detected in the CSF samples of all 6 children, in 2 of the 3 blood samples, and in all of 4 stool samples. The G typing of GARV from 12 of a total of 13 samples indicated that G3 was the predominant G type in all samples. GARV antigens were detected by enzyme-linked immunosorbent assay in all of the 3 tested sera samples, while no GARV antigens were detected in any of the 5 tested CSF samples. We confirmed the presence of GARV genomes in the CSF samples from all of the children with rotavirus-associated seizures, including encephalopathy. However, the relationship between convulsions and the existence of GARV RNA in CSF remains unclear and further study is required.
Liu
Bisei
B
Department of Pediatrics and Child Health, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan.
Fujita
Yukihiko
Y
Arakawa
Chikako
C
Kohira
Ryutaro
R
Fuchigami
Tatsuo
T
Mugishima
Hideo
H
Kuzuya
Mitsutaka
M
D000956:Antigens, Viral / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D003967:Diarrhea / Q000209:etiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D012367:RNA, Viral / Q000097:blood* / Q000134:cerebrospinal fluid*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000150:complications*; D012640:Seizures / Q000209:etiology*
D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus