Headspace solid-phase microextraction-gas chromatography method for the determination of valproic acid in human serum, and formulations using hollow-fiber coated wire.
A method was developed for the extraction of valproic acid (VPA) by hollow-fiber coated wire as a lab-made solid-phase microextraction (SPME) fiber and its determination by capillary gas chromatography in human serum and pharmaceutical formulations. In this study, a piece of copper wire coated by polypropylene hollow-fiber membrane was used as a SPME fiber, and its efficiency for the extraction of VPA from the headspace of samples prior to gas chromatographic analysis was evaluated. The optimum conditions of microextraction process were selected, and the limit of detection for VPA was found to be 85 microg L(-1) in solution and 1.7 mg L(-1) in human serum. A low detection limit, a wide linear dynamic range (0.25-100 mg L(-1)), good repeatability (RSD%<4 in formulations and RSD%<7 in serum samples) and a higher mechanical durability due to its metallic base are some of the most important advantages of the proposed fiber.
Farajzadeh
Mir Ali
MA
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Iran. mafarajzadeh@yahoo.com
Farhadi
Khalil
K
Matin
Amir A
AA
Hashemi
Paria
P
Jouyban
Abolghasem
A
D000927:Anticonvulsants / Q000097:blood / Q000302:isolation & purification; D001774:Blood Chemical Analysis / Q000379:methods*; D002626:Chemistry, Pharmaceutical; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000295:instrumentation* / Q000379:methods*; D012995:Solubility; D013696:Temperature; D013997:Time Factors; D014635:Valproic Acid / Q000097:blood* / Q000302:isolation & purification*
D000927:Anticonvulsants; D004364:Pharmaceutical Preparations; D012492:Salts; D014635:Valproic Acid