title: [Effect of borneol/mentholum eutectic mixture on nasal-brain delivery of neurotoxin loaded nanoparticles].
abstract: OBJECTIVE
  To investigate the absorption enhancen effect of borneol/mentholum eutectic mixture (BO/ME) on nasal-brain delivery of neurotoxin loaded nanoparticles.
  
  
  METHODS
  Using microdialysis sampling technique in awake freely-moving rats, the counter per minute (cpm) of dialysates in right PAG of NT-loaded nanoparticles with the BO/ME (BO/ME-NT-NP), radiolabeled with sodium 125I-Iodide, were measured in a gamma-counter for radioactivity. After converting cpm into corresponding concentrations of NT byin vivorecovery of microdialysis probes, the pharmacokinetic parameters were calculated.
  
  
  RESULTS
  The BO/ME-NT-NP could be absorbed into the brain, much better to NT-NP and the nanoparticles with borneol or menthdlum only, and the pharmacokinetics accorded with the two-compartment model. The parameters tmax, cmax, AUC, t 1/2(beta) were 0.68 h, 27.32 ng x mL(-1), 132.68 ng x h x mL(-1), 3.1076 h.
  
  
  CONCLUSIONS
  With adding BO/ME as absorption enhancer, NT could be significantly increased in the brain with the help of nanopartilces as carriers, and the time to maximal concentration was short, the elimination process was prolonged.
authors.affiliation: College of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China.
authors.forename: Guobao
authors.initials: G
authors.lastname: Chai
authors.forename: Yuefang
authors.initials: Y
authors.lastname: Pan
authors.forename: Fanzhu
authors.initials: F
authors.lastname: Li
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001892:Camphanes / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000493:pharmacokinetics*; D008297:Male; D008610:Menthol / Q000737:chemistry / Q000494:pharmacology*; D017551:Microdialysis; D053758:Nanoparticles; D009296:Nasal Cavity / Q000378:metabolism*; D009498:Neurotoxins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats
chemical_list: D001892:Camphanes; D004337:Drug Carriers; D009498:Neurotoxins; D008610:Menthol; C022871:isoborneol