title: A HindIII polymorphism of fibronectin gene is associated with nephrolithiasis.
abstract: OBJECTIVE
  To evaluate the association between fibronectin gene (FN1) polymorphisms and calcium oxalate nephrolithiasis as a genetic risk factor.
  
  
  METHODS
  Genomic DNA of 143 patients with calcium oxalate nephrolithiasis and 154 healthy controls were screened for polymorphisms (HaeIII b, MspI, and HindIII) of the FN1 gene, using polymerase chain reaction-restriction fragments length polymorphism method. Allele and genotype frequencies were compared between the groups.
  
  
  RESULTS
  Although the observed differences between distribution of genotypes of AA, AB, and BB (for HaeIII b), as well as CC, CD, and DD (MspI) were not significant, FF genotype for HindIII showed significant difference when compared with both EF and EE + EF genotype (P = .00202 and P = .00203, respectively).
  
  
  CONCLUSIONS
  The results of our study revealed that HindIII polymorphism of the FN1 gene is highly associated with calcium oxalate stone disease. This association makes FN a good candidate for further studies about the etiology of stone disease, and in the future it could be a candidate marker for evaluating the genetic risks in patients with nephrolithiasis.
authors.affiliation: Department of Urology, School of Medicine, Gazi University, Ankara, Turkey. monaran@gazi.edu
authors.forename: Metin
authors.initials: M
authors.lastname: Onaran
authors.forename: Akin
authors.initials: A
authors.lastname: Yilmaz
authors.forename: Ilker
authors.initials: I
authors.lastname: Sen
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Ergun
authors.forename: Ahmet
authors.initials: A
authors.lastname: Camtosun
authors.forename: Bora
authors.initials: B
authors.lastname: Küpeli
authors.forename: Sevda
authors.initials: S
authors.lastname: Menevşe
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Bozkirli
mesh_terms: D000328:Adult; D000368:Aged; D002129:Calcium Oxalate / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000235:genetics; D019003:Deoxyribonuclease HpaII / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D053040:Nephrolithiasis / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D005353:Fibronectins; D002129:Calcium Oxalate; D015247:Deoxyribonuclease HindIII; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases