title: Development of inlaid electrodes for whole column electrochemical detection in HPLC.
abstract: An electrochemical microfluidic device has been fabricated on PET (polyethylene terephthalate) substrate using an imprinting method. The imprinting transfers patterns from a stamp into a substrate mechanically. However, a blanket mould imprinting process has been introduced to embed the photolithographically produced gold metal electrode lines into the PET substrate resulting in an individually addressable array flush to better than 100 nm. The device formed one wall of a packed chromatography column. The array was electrochemically characterised using standard redox probes in both stagnant conditions and under flow. Both numerical modelling and experimental data show improved sensitivity under flow and a limiting current which scaled linearly with the cube root of the volume flow rate. A chromatographic separation of the bioanalytical significant neurotransmitter dopamine (DA) and its metabolite DOPAC was achieved and electrochemically detected at multiple locations within the column. The PET device was stable and robust to leaks to pressures well in excess of those required for chromatographic separations.
authors.affiliation: Micro Biochip Center, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Seo
authors.forename: Pei Ling
authors.initials: PL
authors.lastname: Leow
authors.forename: Si-Hyeong
authors.initials: SH
authors.lastname: Cho
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Lim
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Bhavik Anil
authors.initials: BA
authors.lastname: Patel
authors.forename: Jin-Goo
authors.initials: JG
authors.lastname: Park
authors.forename: Danny
authors.initials: D
authors.lastname: O'Hare
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D004298:Dopamine / Q000302:isolation & purification; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D011093:Polyethylene Terephthalates / Q000737:chemistry
chemical_list: D011093:Polyethylene Terephthalates; D015102:3,4-Dihydroxyphenylacetic Acid; D004298:Dopamine