Dry-powder pulmonary insufflation in the mouse for application to vaccine or drug studies.
Pulmonary delivery of substances in small animal models is often useful for experimental testing of various vaccine and drug candidates. One of the most challenging elements to such protocols is the efficient disposition of test materials in the lungs of mice. Herein we detail a means to deliver dry powders of an inhalant live-attenuated Mycobacterium bovis Bacille Calmette-Guerin (BCG) vaccine against Mycobacterium tuberculosis to the lungs of mice. The direct delivery methodology is quick, safe, and allows for repeated pulmonary insufflation of substances if boosting is desired. This model system could be easily adapted for use with other dry-powder vaccine and drug candidates.
Morello
Melissa
M
Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, GA 30602, USA.
Krone
Cassandra L
CL
Dickerson
Shelley
S
Howerth
Elizabeth
E
Germishuizen
Willem Andreas
WA
Wong
Yun-Ling
YL
Edwards
David
D
Bloom
Barry R
BR
Hondalus
Mary K
MK
D000284:Administration, Oral; D000336:Aerosols; D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D007327:Insufflation; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology / Q000472:pathogenicity*; D011208:Powders
D000336:Aerosols; D001500:BCG Vaccine; D011208:Powders