High-performance liquid-chromatographic separation of ofloxacin using a chiral stationary phase.
A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm x 4.6 mm, 5 microm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers.
Sun
Xiaojie
X
Central Laboratory, Beijing Center for Disease Prevention & Control, China.
Wu
Di
D
Shao
Bing
B
Zhang
Jing
J
D002219:Carbamates / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D015242:Ofloxacin / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism; D013696:Temperature
D002219:Carbamates; D002482:Cellulose; D015242:Ofloxacin