[Up-regulation of Fas is related to apoptosis of HeLa cells induced by co-immobilized TNF-alpha/IFN-gamma].
This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
Sun
Ruifang
R
College of Life Science, South China Normal University, Guangzhou 510631, China.
Fang
Zeqin
Z
Zheng
Jianting
J
Wu
Zherui
Z
Qiu
Jinwei
J
Li
Weifang
W
Zhao
Dongqin
D
Liang
Shujun
S
Guan
Yanqing
Y
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D004357:Drug Synergism; D006367:HeLa Cells; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D007371:Interferon-gamma / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000494:pharmacology*; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism*
C491901:FAS protein, human; D055767:Immobilized Proteins; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D007371:Interferon-gamma