title: Remyelination in experimentally demyelinated connexin 32 knockout mice.
abstract: The aim of this study was to evaluate the role of connexin 32 (Cx 32) during remyelination of the peripheral nervous system, through a local injection of either 0,1% ethidium bromide solution or saline in the sciatic nerve of Cx 32 knockout mice. Euthanasia was performed ranging from 1, 2, 3, 7, 15, 21 to 30 days after injection. Histochemical, immunohistochemical, immunofluorescence and transmission electron microscopical techniques were used to analyze the development of the lesions. Within the sciatic nerves, Schwann cells initially showed signs of intoxication and rejected their sheaths; after seven days, some thin newly formed myelin sheaths with uneven compactness and redundant loops (tomacula) were conspicuous. We concluded that the regeneration of lost myelin sheaths within the PNS followed the pattern already reported for this model in other laboratory species. Therefore, these results suggest that absence of Cx 32 did not interfere with the normal pattern of remyelination in this model in young mice.
authors.affiliation: Laboratory of Veterinary Pathology, Departament of Pathology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Adriano Tony
authors.initials: AT
authors.lastname: Ramos
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Maiorka
authors.forename: Maria Lúcia Zaidan
authors.initials: ML
authors.lastname: Dagli
authors.forename: Fernando Yutaka Moniwa
authors.initials: FY
authors.lastname: Hosomi
authors.forename: Kalan Bastos
authors.initials: KB
authors.lastname: Violin
authors.forename: Andréa
authors.initials: A
authors.lastname: Latorre
authors.forename: Aline de Marco
authors.initials: Ade M
authors.lastname: Viott
authors.forename: Eduardo Kenji
authors.initials: EK
authors.lastname: Masuda
authors.forename: Maria Elisa
authors.initials: ME
authors.lastname: Trost
authors.forename: Tessie Beck
authors.initials: TB
authors.lastname: Martins
authors.forename: Dominguita Lühers
authors.initials: DL
authors.lastname: Graça
mesh_terms: D000818:Animals; D017630:Connexins / Q000502:physiology*; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000503:physiopathology*; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D009186:Myelin Sheath / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D000097002:Gap Junction beta-1 Protein
chemical_list: D017630:Connexins; D000097002:Gap Junction beta-1 Protein