Theoretical advantages and drawbacks of on-line, multidimensional liquid chromatography using multiple columns operated in parallel.
The theoretical advantages and drawbacks of using a multiple-, parallel column approach in on-line multidimensional liquid chromatography systems were investigated. Much time or peak capacity can be gained with the use of multiple parallel columns at the second-dimension while the aggregate time of separation increases only by the increment of the gradient time of the second-dimension. Multidimensional chromatographic systems are now used to perform many tasks ranging from routine, fast analyses to specialized, arduous separations. In this work, we focus on the advantages of a multiple, parallel columns approach to on-line multidimensional liquid chromatography systems. Calculations of the achievable peak capacities were made as functions of the number of columns operated in parallel. Increasing the number of second-dimension columns from one to two or three causes the largest increase in peak capacity with only a slight increase of aggregate time. We also present some practical aspects to consider when attempting multidimensional separations with multiple columns operated in parallel.
Fairchild
Jacob N
JN
Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
Horváth
Krisztián
K
Guiochon
Georges
G
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008962:Models, Theoretical; D013997:Time Factors