title: Evolution of oxidative/nitrosative stress biomarkers during an open-field vaccination procedure in sheep: effect of melatonin.
abstract: Melatonin has been shown to exert immunomodularory properties with broad application in veterinary medicine. In previous work we have described that subcutaneous coadministration of melatonin to seeps vaccinated against two stumps of A1 and C strains of Dichelobacter nodosus enhanced both the antibody titer and serum IgG levels to A1 and C strains of D. nodosus compared to vaccinated animals not treated with melatonin. Following a similar protocol here we have investigated the effect of a higher dose of melatonin (36mg/animal) in the improvement of the immune response and in the possible oxidative/nitrosative stress produced during the immunization protocol. Our results show that footrot vaccine application induced nitrosative but not oxidative stress at 42 days post-vaccination, which was neutralized by melatonin administration. On the other hand, melatonin improved the immune response with respect to our previous data increasing the time of permanence of antibodies in serum, opening new perspectives for melatonin as prophylactic drug.
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, University of Extremadura, Avda. de la Universidad s/n, 10071 Cáceres, Spain. mpmiguez@unex.es
authors.forename: Asunción
authors.initials: A
authors.lastname: Ramos
authors.forename: Inés
authors.initials: I
authors.lastname: Laguna
authors.forename: María-Luisa Martín
authors.initials: ML
authors.lastname: de Lucía
authors.forename: Pedro
authors.initials: P
authors.lastname: Martín-Palomino
authors.forename: Sergio
authors.initials: S
authors.lastname: Regodón
authors.forename: María-Prado
authors.initials: MP
authors.lastname: Míguez
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000975:Antioxidants / Q000494:pharmacology; D001428:Bacterial Vaccines / Q000276:immunology* / Q000494:pharmacology; D001831:Body Temperature / Q000276:immunology; D003692:Delayed-Action Preparations; D020376:Dichelobacter nodosus / Q000276:immunology*; D005260:Female; D005535:Foot Rot / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D005978:Glutathione / Q000097:blood; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D008315:Malondialdehyde / Q000097:blood; D008550:Melatonin / Q000494:pharmacology*; D009566:Nitrates / Q000097:blood; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000975:Antioxidants; D001428:Bacterial Vaccines; D003692:Delayed-Action Preparations; D009566:Nitrates; D008315:Malondialdehyde; D005978:Glutathione; D008550:Melatonin