title: Electron microscopy of helical filaments: rediscovering buried treasures in negative stain.
abstract: Although negative stain electron microscopy is a wonderfully simple way of directly visualizing protein complexes and other biological macromolecules, the images are not really comparable to those of objects seen in everyday life. The failure to appreciate this has recently led to an incorrect interpretation of RecA-family filament structures.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908-0733, USA. egelman@virginia.edu
authors.forename: Edward H
authors.initials: EH
authors.lastname: Egelman
authors.forename: Linda A
authors.initials: LA
authors.lastname: Amos
mesh_terms: D004247:DNA / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D011938:Rec A Recombinases / Q000648:ultrastructure
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA; D011938:Rec A Recombinases