title: Osteogenic and adipogenic differentiation potential  of an immortalized fibroblast-like cell line derived  from porcine peripheral blood.
abstract: Primary fibroblast-like cells isolated from the peripheral blood of a healthy pig were immortalized by transduction of cells with a replication-defective retrovirus vector expressing the E6/E7 proteins of human papillomavirus type 16 (pLXSN-16E6E7). The immortalized cells grow rapidly in cell culture and exhibit a distinct cell surface phenotype that was positive for CD90, CD44, collagen I, and vimentin and negative for CD14 and MHC II. Additionally, these immortalized blood derived-fibroblast-like cells had the potential to differentiate into osteoblasts and adipocytes in vitro as evidenced by the deposition of calcium, increased alkaline phosphatase activity, upregulated osteogenic and adipogenic marker gene expression, and accumulation of fat droplets in cells when osteogenic (dexamethasone, ascorbic acid, and β-glycerophosphate) or adipogenic supplements (dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin, and insulin) were added to the culture. Overall, the results suggest that the immortalized blood-derived fibroblast-like cells exhibit some of the features of mesenchymal precursor cells, which may have implications in tissue repair and remodeling process.
authors.affiliation: Department of Biology and Microbiology, Center for Infectious Disease Research and Vaccinology, South Dakota State University, NPB 252D, Box 2140D, Brookings, SD 57007, USA. xiuqing.wang@sdstate.edu
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Wang
authors.forename: Daphne
authors.initials: D
authors.lastname: Moutsoglou
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D005347:Fibroblasts / Q000166:cytology*; D005819:Genetic Markers; D009856:Oncogene Proteins, Viral / Q000235:genetics; D010011:Osteocytes / Q000166:cytology*; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D010641:Phenotype; D012097:Repressor Proteins / Q000235:genetics; D013552:Swine
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D005819:Genetic Markers; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16