title: Chloride channels involve in hydrogen peroxide-induced apoptosis of PC12 cells.
abstract: Chloride channel activity is one of the critical factors responsible for cell apoptotic volume decrease (AVD). However, the roles of chloride channels in apoptosis have not been fully understood. In the current study, we assessed the role of chloride channels in hydrogen peroxide (H(2)O(2))-induced apoptosis of pheochromocytoma cells (PC12). Extracellular application of H(2)O(2) activated a chloride current and induced cell volume decrease in a few minutes. Incubation of cells with H(2)O(2) elevated significantly the membrane permeability to the DNA dye Hoechst 33258 in 1h and induced apoptosis of most PC12 cells tested in 24h. The chloride channel blocker NPPB (5-nitro-2-(3-phenylpropylamino)-benzoate) prevented appearance of H(2)O(2)-induced high membrane permeability and cell shrinkage, suppressed H(2)O(2)-activated chloride currents and protected PC12 cells from apoptosis induced by H(2)O(2). The results suggest that chloride channels may contribute to H(2)O(2)-induced apoptosis by ways of elevation of membrane permeability and AVD in PC12 cells.
authors.affiliation: Department of Physiology, Jinan University, Guangzhou, China.
authors.forename: Wanhong
authors.initials: W
authors.lastname: Zuo
authors.forename: Linyan
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Bai
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianwen
authors.initials: J
authors.lastname: Mao
authors.forename: Lixin
authors.initials: L
authors.lastname: Chen
authors.forename: Liwei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D009579:Nitrobenzoates / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats
chemical_list: D018118:Chloride Channels; D009579:Nitrobenzoates; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D006861:Hydrogen Peroxide