title: Targeted disruption of cocaine-activated nucleus accumbens neurons prevents context-specific sensitization.
abstract: Learned associations between effects of abused drugs and the drug administration environment are important in drug addiction. Histochemical and electrophysiological studies suggest that these associations are encoded in sparsely distributed nucleus accumbens neurons that are selectively activated by drugs and drug-associated cues. Although correlations have been observed between nucleus accumbens neuronal activity and responsivity to drugs and drug cues, no technique exists for selectively manipulating these activated neurons and establishing their causal role in behavioral effects of drugs and drug cues. Here we describe a new approach, which we term the 'Daun02 inactivation method', that selectively inactivates a minority of neurons previously activated by cocaine in an environment repeatedly paired with cocaine to demonstrate a causal role for these activated neurons in context-specific cocaine-induced psychomotor sensitization in rats. This method provides a new tool for studying the causal roles of selectively activated neurons in behavioral effects of drugs and drug cues and in other learned behaviors.
authors.affiliation: Behavioral Neuroscience Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, Maryland, USA.
authors.forename: Eisuke
authors.initials: E
authors.lastname: Koya
authors.forename: Sam A
authors.initials: SA
authors.lastname: Golden
authors.forename: Brandon K
authors.initials: BK
authors.lastname: Harvey
authors.forename: Danielle H
authors.initials: DH
authors.lastname: Guez-Barber
authors.forename: Alexander
authors.initials: A
authors.lastname: Berkow
authors.forename: Danielle E
authors.initials: DE
authors.lastname: Simmons
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Bossert
authors.forename: Sunila G
authors.initials: SG
authors.lastname: Nair
authors.forename: Jamie L
authors.initials: JL
authors.lastname: Uejima
authors.forename: Marcelo T
authors.initials: MT
authors.lastname: Marin
authors.forename: Timothy B
authors.initials: TB
authors.lastname: Mitchell
authors.forename: David
authors.initials: D
authors.lastname: Farquhar
authors.forename: Sukhen C
authors.initials: SC
authors.lastname: Ghosh
authors.forename: Brandi J
authors.initials: BJ
authors.lastname: Mattson
authors.forename: Bruce T
authors.initials: BT
authors.lastname: Hope
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D003042:Cocaine / Q000494:pharmacology*; D019970:Cocaine-Related Disorders / Q000503:physiopathology*; D003463:Cues; D003630:Daunorubicin / Q000633:toxicity; D004195:Disease Models, Animal; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D007858:Learning / Q000187:drug effects / Q000502:physiology; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D009480:Neuropharmacology / Q000379:methods; D009498:Neurotoxins / Q000633:toxicity; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000502:physiology; D011597:Psychomotor Performance / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic
chemical_list: D018765:Dopamine Uptake Inhibitors; D009498:Neurotoxins; D003042:Cocaine; D003630:Daunorubicin