title: Enzyme synthesis of oligosaccharides using cashew apple juice as substrate.
abstract: The use of agriculture substrates in industrial biotechnological processes has been increasing because of their low cost. In this work, the use of clarified cashew apple juice was investigated as substrate for enzyme synthesis of prebiotic oligosaccharide. The results showed that cashew apple juice is a good source of reducing sugars and can be used as substrate for the production of dextransucrase by Leuconostoc citreum B-742 for the synthesis of oligosaccharides using the crude enzyme. Optimal oligosaccharide yield (approximately 80%) was obtained for sucrose concentrations lower than 60 g/L and reducing sugar concentrations higher than 100 g/L.
authors.affiliation: Departamento de Tecnologia de Alimentos (UFC), Campus do Pici, Bloco 858, Caixa Postal 12168, CEP 60021-970, Fortaleza-CE, Brazil.
authors.forename: Maria Cristiane
authors.initials: MC
authors.lastname: Rabelo
authors.forename: Claudia P M L
authors.initials: CP
authors.lastname: Fontes
authors.forename: Sueli
authors.initials: S
authors.lastname: Rodrigues
mesh_terms: D000383:Agriculture / Q000379:methods*; D031021:Anacardium; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D019649:Food Industry; D005964:Glucosyltransferases / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000378:metabolism; D027845:Malus; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D002241:Carbohydrates; D009844:Oligosaccharides; D005964:Glucosyltransferases; C020025:dextransucrase; D005990:Glycerol