[Preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method].
OBJECTIVE
To evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms.


METHODS
Suspensions of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum with same density were prepared and cultured respectively at liquid BHI medium. Then the growth quantity was measured periodically through turbidimetry and the growth curves of the inoculated bacteria were completed. The culture solutions of stable growth phase were sampled and characterized by 1H-nuclear magnetic resonance 1H-NMR). The data of 1H-NMR spectroscope results were analyzed by principal components analysis (PCA).


RESULTS
The PCA showed the obvious clustering phenomena and the points of three groups differentially centralized to three clusters. Therefore, the NMR-based metabonomics profiles could discriminate the three different kinds of bacteria.


CONCLUSIONS
The metabonomics is a potential classable method to identify the oral pathogenic bacteria.
Lu
Wei-Xi
WX
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Wu
Ya-Fei
YF
Xiao
Li-Ying
LY
Li
Ming-Yun
MY
Guo
Qiang
Q
Xiong
Ping
P
Jia
Xiang-Ming
XM
Xiao
Xiao-Rong
XR
Zhu
Zhu
Z
Gong
Qi-Mei
QM
Li
Wei
W
D016976:Aggregatibacter actinomycetemcomitans; D001419:Bacteria; D016967:Fusobacterium nucleatum; D055432:Metabolomics; D009055:Mouth / Q000382:microbiology; D016966:Porphyromonas gingivalis; D018713:Prevotella intermedia