Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer.
Third generation cationic dendritic polymeric polypropyleneimine (PPI) was modified by Pluronic P123 and investigated for gene delivery. The cytotoxicity of P123-PPI was evaluated by the MTT assay and shown to be much lower than that of PPI alone. P123-PPI and PPI can both condense plasmid DNA into nanoparticles with a size of approximately 100 nm and a zeta potential of about 15 mV at the N/P ratio 20:1. The nanoparticles can protect plasmid DNA from being digested by DNase I at a concentration of 0.4 U/microg DNA. The nanoparticles were resistant to dissociation induced by 50% fetal bovine serum and 75 microg/mL sodium heparin. The transfection efficiency of SPC-A1 cells using P123-PPI/DNA nanoparticles was much higher than the transfection utilizing PPI/DNA nanoparticles. The addition of free P123 during the preparation of P123-PPI/DNA nanoparticles could significantly enhance the transfection efficiency in the presence of 10% fetal bovine serum. Therefore, P123-PPI/DNA complex nanoparticles may be a safe, efficient and promising cationic conjugate for gene delivery.
Hao
Junguo
J
Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, 201203, China.
Sha
Xianyi
X
Tang
Yuanjia
Y
Jiang
Ye
Y
Zhang
Zhiwen
Z
Zhang
Wei
W
Li
Yajuan
Y
Fang
Xiaoling
X
D021581:Active Transport, Cell Nucleus; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry* / Q000633:toxicity; D003850:Deoxyribonuclease I / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006493:Heparin / Q000378:metabolism; D006801:Humans; D053758:Nanoparticles; D009690:Nucleic Acid Conformation; D011060:Poloxalene / Q000737:chemistry* / Q000633:toxicity; D011126:Polypropylenes / Q000737:chemistry* / Q000633:toxicity; D044967:Serum / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D050091:Dendrimers; D011126:Polypropylenes; C485184:enhanced green fluorescent protein; C443641:poly(propyleneimine); D049452:Green Fluorescent Proteins; C464484:pluronic block copolymer P123; D011060:Poloxalene; D006493:Heparin; D004247:DNA; D003850:Deoxyribonuclease I