[Use of soybean trypsin inhibitor for modification of gold surface of the sensor chips in surface plasmon resonance spectrometer].
Kunitz soybean trypsin inhibitor (STI) and glutaraldehyde are used to create an interlayer for proteins immobilization on the gold surface of the sensor chips of surface plasmon resonance spectrometer. Human serum albumin (HSA), goat Ig G and bovine eye lens alpha-crystallin are immobilized via the proposed interlayer. We studied the effects of the duration of storage of the sensor chips before use and pre-treatment by "piranha" solution on the STI adsorption by the gold surface. The influence of STI surface concentration, as well as the effect of the duration of storage of STI-modified sensor chips on the HSA immobilization are investigated. The binding of specific antibodies to the immobilized proteins and non-specific binding to the modified surfaces are studied. HSA immobilization on the bare gold surface is compared to that on the surfaces, modified by different methods.
Avilov
S V
SV
Aleksandrova
N O
NO
Kunda
Ie M
IeM
Verevka
S V
SV
Shyrshov
Iu M
IuM
D000818:Animals; D002417:Cattle; D005976:Glutaral / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000737:chemistry*; D038201:alpha-Crystallins / Q000032:analysis
D055767:Immobilized Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin; D038201:alpha-Crystallins; D012710:Serum Albumin, Bovine; D006046:Gold; D014360:Trypsin Inhibitor, Kunitz Soybean; D005976:Glutaral