title: Lung cancer gene expression database analysis incorporating prior knowledge with support vector machine-based classification method.
abstract: BACKGROUND
  A reliable and precise classification is essential for successful diagnosis and treatment of cancer. Gene expression microarrays have provided the high-throughput platform to discover genomic biomarkers for cancer diagnosis and prognosis. Rational use of the available bioinformation can not only effectively remove or suppress noise in gene chips, but also avoid one-sided results of separate experiment. However, only some studies have been aware of the importance of prior information in cancer classification.
  
  
  METHODS
  Together with the application of support vector machine as the discriminant approach, we proposed one modified method that incorporated prior knowledge into cancer classification based on gene expression data to improve accuracy. A public well-known dataset, Malignant pleural mesothelioma and lung adenocarcinoma gene expression database, was used in this study. Prior knowledge is viewed here as a means of directing the classifier using known lung adenocarcinoma related genes. The procedures were performed by software R 2.80.
  
  
  RESULTS
  The modified method performed better after incorporating prior knowledge. Accuracy of the modified method improved from 98.86% to 100% in training set and from 98.51% to 99.06% in test set. The standard deviations of the modified method decreased from 0.26% to 0 in training set and from 3.04% to 2.10% in test set.
  
  
  CONCLUSIONS
  The method that incorporates prior knowledge into discriminant analysis could effectively improve the capacity and reduce the impact of noise. This idea may have good future not only in practice but also in methodology.
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110001, PR China. pguan@mail.cmu.edu.cn
authors.forename: Peng
authors.initials: P
authors.lastname: Guan
authors.forename: Desheng
authors.initials: D
authors.lastname: Huang
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.forename: Baosen
authors.initials: B
authors.lastname: Zhou
mesh_terms: D001185:Artificial Intelligence; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*