Significant inverse correlation of microRNA-150/MYB and microRNA-222/p27 in myelodysplastic syndrome.
We investigated whether, in myelodysplastic syndromes (MDS), aberrant expression of miR-150/miR-221/miR-222 and their designated target mRNA molecules MYB, p27 and c-KIT may be involved in insufficient haematopoiesis. In a series of MDS (n=52), an aberrant increase of miR-150 was found only in MDS with associated del(5q) (n=9; p<0.01). The mRNA expression of transcription factor MYB, the designated target of miR-150, was shown to correlate inversely with the miR-150 level. Acute leukaemia evolving from MDS (n=11) showed significantly decreased levels of miR-221 but not miR-222. We conclude that inhibition of proliferation via over-expressed miR-150 might contribute to myelodysplastic haematopoiesis in MDS-del(5q).
Hussein
Kais
K
Institute of Pathology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Hussein.Kais@MH-Hannover.de
Theophile
Katharina
K
Büsche
Guntram
G
Schlegelberger
Brigitte
B
Göhring
Gudrun
G
Kreipe
Hans
H
Bock
Oliver
O
D000368:Aged; D002869:Chromosome Aberrations; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000378:metabolism; D020626:Oncogene Proteins v-myb / Q000096:biosynthesis; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis
D035683:MicroRNAs; D020626:Oncogene Proteins v-myb; D019009:Proto-Oncogene Proteins c-kit; C533097:MIR150, human; C525709:MIR221, human; C525710:MIR222, human