title: Identification of the source of histoplasmosis infection in two captive maras (Dolichotis patagonum) from the same colony by using molecular and immunologic assays.
abstract: Histoplasma capsulatum was isolated from the spleen of a first infected mara (Dolichotis patagonum) and from a second mara's liver and adrenal gland, both in the same colony at the Africam Safari, Puebla, Mexico. Studies of H. capsulatum isolates, using nested-PCR of a 100-kDa protein coding gene (Hcp100) fragment and a two-primer RAPD-PCR method, suggest that these isolates were spreading in the environment of the maras' enclosure. By using a Dot-ELISA method, sera from mice inoculated with three homogenates of soil samples from the maras' enclosed space developed positive brown spot reactions to a purified H. capsulatum antigen, which identified the probable source of the maras' infection.
authors.affiliation: Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, DF Mexico.
authors.forename: M R
authors.initials: MR
authors.lastname: Reyes-Montes
authors.forename: G
authors.initials: G
authors.lastname: Rodríguez-Arellanes
authors.forename: A
authors.initials: A
authors.lastname: Pérez-Torres
authors.forename: A G
authors.initials: AG
authors.lastname: Rosas-Rosas
authors.forename: A
authors.initials: A
authors.lastname: Parás-García
authors.forename: C
authors.initials: C
authors.lastname: Juan-Sallés
authors.forename: M L
authors.initials: ML
authors.lastname: Taylor
mesh_terms: D000311:Adrenal Glands / Q000382:microbiology; D000818:Animals; D001717:Birds / Q000382:microbiology; D002685:Chiroptera / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis; D004197:Disease Reservoirs / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006658:Histoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006799:Housing, Animal; D008099:Liver / Q000382:microbiology; D008800:Mexico / Q000453:epidemiology; D051379:Mice; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012376:Rodent Diseases / Q000453:epidemiology / Q000382:microbiology*; D012377:Rodentia / Q000382:microbiology*; D012988:Soil Microbiology; D013154:Spleen / Q000382:microbiology
chemical_list: D004271:DNA, Fungal