An in-house-anti-hepatitis A virus (HAV)-specific immunoglobulin M capture enzyme-linked immunosorbent assay: evaluation and application to an HAV outbreak.
An anti-hepatitis A virus (HAV)-specific immunoglobulin M capture enzyme-linked immunosorbent assay (anti-HAV IgM ELISA) kit was re-designed for laboratory use and compared with a commercial anti-HAV IgM detection system using 58 serum samples collected from patients, vaccines, and healthy individuals. Because concordance between the two systems was high (r = 0.93, P < 0.05), 19 sets of serum and fecal samples obtained from individuals exposed to an HAV outbreak were also examined. Serum levels of anti-HAV IgM were determined using the in-house ELISA kit and the HAV genome in fecal samples was detected using the polymerase chain reaction (PCR). Among the 19 sets of sample, 14 were positive for both anti-HAV IgM and the HAV genome. All of those whose serum samples were anti-HAV IgM negative were also negative for the HAV genome in fecal samples. The results of the in-house IgM ELISA were consistent with those of the HAV genome detected by PCR and with the commercial IgM ELISA. The in-house anti-HAV IgM ELISA kit was therefore proven suitable for laboratory use and applicable to epidemiological studies of HAV infection.
Kiyohara
Tomoko
T
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan. kiyohara@nih.go.jp
Ouchi
Yoshimi
Y
Hasegawa
Yoshiko
Y
Sato
Tomoko
T
Yoneyama
Tetsuo
T
Ishii
Koji
K
Ito
Toshihiro
T
Wakita
Takaji
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000302:isolation & purification; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000821:virology; D006506:Hepatitis A / Q000175:diagnosis* / Q000453:epidemiology*; D035922:Hepatitis A Antibodies; D030041:Hepatitis A virus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D035922:Hepatitis A Antibodies; D007075:Immunoglobulin M