An adenovirus-delivered peptide aptamer C1-1 targeting the core protein of hepatitis B virus inhibits viral DNA replication and production in vitro and in vivo.
Peptide aptamers are molecules which can specifically bind to a given target protein and have the potential to selectively block the function of the target protein. It has been reported that a peptide aptamer (C1-1) identified from a randomized expression library specifically bound to the core protein of hepatitis B virus and inhibited viral capsid formation and DNA replication in vitro. Adenoviral systems are popular platforms for reliable gene delivery and high-level transient expression in any mammalian cell type in vitro, and have a natural tropism for the liver after systemic administration. In the present study, we explored the feasibility of gene therapy against HBV infection with adenoviral system, and found that systematic administration of recombinant adenovirus encoding the peptide aptamer (C1-1) significantly inhibited viral capsid formation, HBV DNA replication and virion production in vivo. These results suggest an efficient antiviral treatment against HBV infection by delivery of anti-HBV peptide aptamer with recombinant adenovirus.
Zhang
Wei
W
Laboratory of Cardiovascular Diseases, West China Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, People's Republic of China.
Ke
Wei
W
Wu
Si-Si
SS
Gan
Lu
L
Zhou
Rui
R
Sun
Chang-Yan
CY
Long
Qing-Shan
QS
Jiang
Wei
W
Xin
Hong-Bo
HB
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D052158:Aptamers, Peptide / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism*
D052158:Aptamers, Peptide; D004279:DNA, Viral; D014758:Viral Core Proteins; C438062:peptide aptamer C1-1