title: Gold electrode incorporating corrole as an ion-channel mimetic sensor for determination of dopamine.
abstract: Here, we report on an ion-channel mimetic sensor using self-assembly monolayers deposited onto gold electrodes for electrochemical determination of dopamine. The different compositions of the modification solution consist of corrole-SH and other thiol derivatives used as the "background compounds" such as 1-dodecanethiol (DDT), 6-mercapto-1-hexanol (HS(CH(2))(6)OH), or 11-mercapto-1-undecanol (HS(CH(2))(11)OH) were explored to find the best self-assembled monolayer (SAM) suitable for dopamine determination. Among them, the mixed SAM consisting of corroles with the -SH group and 6-mercapto-1-hexanol (HS(CH(2))(6)OH) in the molar ratio 1:10 was the most sensitive. The signals generated by the formation of a complex between the corrole host and the dopamine guest were measured by Osteryoung square-wave voltammetry (OSWV) and electrochemical impedance spectroscopy (EIS) with [Ru(NH(3))(6)](3+) as an electroactive marker. The developed sensor was free of interferences of components of human plasma such as ascorbic acid, creatinine, creatine, and uric acid. The detection limits observed by EIS in buffer solution and in the presence of centrifuged human plasma 80 times diluted with a 0.9% NaCl containing 0.01 M borate buffer solution of pH 7.0 were 3.3 x 10(-12) and 5.3 x 10(-12) M, respectively.
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street 10, 10-747 Olsztyn, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kurzatkowska
authors.forename: Eduard
authors.initials: E
authors.lastname: Dolusic
authors.forename: Wim
authors.initials: W
authors.lastname: Dehaen
authors.forename: Karolina
authors.initials: K
authors.lastname: Sieroń-Stołtny
authors.forename: Aleksander
authors.initials: A
authors.lastname: Sieroń
authors.forename: Hanna
authors.initials: H
authors.lastname: Radecka
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004298:Dopamine / Q000097:blood*; D015259:Dopamine Agents / Q000097:blood*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D011166:Porphyrins / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D015259:Dopamine Agents; D011166:Porphyrins; C431863:corrole; D006046:Gold; D004298:Dopamine