title: Consequences of head injury and static cold storage on hepatic function: ex vivo experiments using a model of isolated perfused rat liver.
abstract: The purpose of the study was to evaluate the effect of head injury (HI) on the metabolic and energy functions of the liver and its consequences after cold storage. In male SD rats with HI, livers were isolated 4 days after injury and perfused either immediately (HI) or after 24 hours of cold preservation. Livers isolated from healthy rats were treated identically. The hepatic functions were explored with an isolated perfused liver model. Head injury induced a liver atrophy without significant difference in the intrahepatic energy level versus healthy rats. After cold storage, hepatic adenosine triphosphate and glycogen contents in HI rats were similar to those of healthy rats. The livers of the HI group that underwent cold preservation had a lower protein catabolism. The portal flow rate at the time of reperfusion was significantly increased in the HI group. In conclusion, static cold storage of livers harvested from HI rats revealed a net protein catabolism reduction and a modification of hepatic microcirculation.
authors.affiliation: Laboratoire de Biologie de la Nutrition EA 2498 Faculté de Pharmacie Université Paris Descartes, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Levesque
authors.forename: Christophe
authors.initials: C
authors.lastname: Moinard
authors.forename: Valérie
authors.initials: V
authors.lastname: Besson
authors.forename: Nathalie
authors.initials: N
authors.lastname: Neveux
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Chaumeil
authors.forename: Luc
authors.initials: L
authors.lastname: Cynober
authors.forename: Christine
authors.initials: C
authors.lastname: Charrueau
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D006259:Craniocerebral Trauma / Q000503:physiopathology*; D015925:Cryopreservation; D004734:Energy Metabolism / Q000502:physiology; D022781:Hepatocytes / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000502:physiology* / Q000503:physiopathology*; D008111:Liver Function Tests; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D019188:Organ Preservation Solutions; D009929:Organ Size / Q000502:physiology; D011169:Portal Vein / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000227:Adenine Nucleotides; D008112:Liver Glycogen; D019188:Organ Preservation Solutions