G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.
We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
Evans
Cory J
CJ
Department of Molecular, Cell and Developmental Biology, Los Angeles, University of California, Los Angeles, Los Angeles, California, USA.
Olson
John M
JM
Ngo
Kathy T
KT
Kim
Eunha
E
Lee
Noemi E
NE
Kuoy
Edward
E
Patananan
Alexander N
AN
Sitz
Daniel
D
Tran
Phuongthao
P
Do
Minh-Tu
MT
Yackle
Kevin
K
Cespedes
Albert
A
Hartenstein
Volker
V
Call
Gerald B
GB
Banerjee
Utpal
U
D000818:Animals; D019070:Cell Lineage; D002999:Clone Cells; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology / Q000235:genetics*; D005470:Fluorometry; D017930:Genes, Reporter; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D016366:Open Reading Frames; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase