title: Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis.
abstract: BACKGROUND
  The polysaccharide of culture medium from Arthrospira platensis was extracted by ultrafiltration, partially characterized and assayed for anticoagulant activity.
  
  
  METHODS
  The crude polysaccharidic fraction was fractionated by anion exchange chromatography on DEAE-cellulose, subjected to acetate cellulose electrophoresis and characterized by physicochemical procedures. The anticoagulant effect of the ultrafiltrated polysaccharide was checked by several coagulation tests.
  
  
  RESULTS
  Anion exchange chromatography revealed in the whole ultrafiltrated polysaccharidic fraction the occurrence of a sulfated spirulan-like component designated PUF2. The average molecular weight of PUF2 was determined by size exclusion chromatography combined with multi-angle light scattering (SEC-MALS) and viscosimetry and was 199 kDa and the sulfate content was 20% weight/dry weight. The physicochemical characterization indicated the occurrence of rhamnose (49.7%), galacturonic and glucuronic acid (32% of total sugar). The anticoagulant effect of this sulfated polysaccharide was mainly due to the potentiation of thrombin inhibition by heparin cofactor II and was 4-times higher than that of the porcine dermatan sulfate whereas it had no effect on anti-Xa activity.
  
  
  CONCLUSIONS
  An ultrafiltrated sulfated polysaccharide, likely a calcium spirulan was obtained from the culture medium of A. platensis and showed an anticoagulant activity mediated by heparin cofactor II.
  
  
  CONCLUSIONS
  Old culture medium of A. platensis may represent an important source for the spirulan-like PUF2 which was endowed with potentially useful anticoagulant properties whereas its obtention by ultrafiltration may represent an extraction procedure of interest.
authors.affiliation: Laboratoire Polymères, Biopolymères et Matériaux Organiques, Faculté des Sciences, Monastir, 5000 Monastir, Tunisia. Hatemmajdoub2002@yahoo.fr
authors.forename: Hatem
authors.initials: H
authors.lastname: Majdoub
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Mansour
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chaubet
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Roudesli
authors.forename: Raoui M
authors.initials: RM
authors.lastname: Maaroufi
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002417:Cattle; D017077:Culture Media, Conditioned / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D015951:Factor Xa / Q000378:metabolism; D065427:Factor Xa Inhibitors; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D053545:Spirulina / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*; D013552:Swine; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism; D013918:Thrombin Time; D014783:Viscosity
chemical_list: D000925:Anticoagulants; D017077:Culture Media, Conditioned; D065427:Factor Xa Inhibitors; D009005:Monosaccharides; D011134:Polysaccharides; D013431:Sulfates; D013917:Thrombin; D015951:Factor Xa