title: Urinary NGAL marks cystic disease in HIV-associated nephropathy.
abstract: Nephrosis and a rapid decline in kidney function characterize HIV-associated nephropathy (HIVAN). Histologically, HIVAN is a collapsing focal segmental glomerulosclerosis with prominent tubular damage. We explored the expression of neutrophil gelatinase-associated lipocalin (NGAL), a marker of tubular injury, to determine whether this protein has the potential to aid in the noninvasive diagnosis of HIVAN. We found that expression of urinary NGAL was much higher in patients with biopsy-proven HIVAN than in HIV-positive and HIV-negative patients with other forms of chronic kidney disease. In the HIV-transgenic mouse model of HIVAN, NGAL mRNA was abundant in dilated, microcystic segments of the nephron. In contrast, urinary NGAL did not correlate with proteinuria in human or in mouse models. These data show that marked upregulation of NGAL accompanies HIVAN and support further study of uNGAL levels in large cohorts to aid in the noninvasive diagnosis of HIVAN and screen for HIVAN-related tubular damage.
authors.affiliation: College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Neal
authors.initials: N
authors.lastname: Paragas
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Nickolas
authors.forename: Christina
authors.initials: C
authors.lastname: Wyatt
authors.forename: Catherine S
authors.initials: CS
authors.lastname: Forster
authors.forename: Meghan
authors.initials: M
authors.lastname: Sise
authors.forename: Susan
authors.initials: S
authors.lastname: Morgello
authors.forename: Bernd
authors.initials: B
authors.lastname: Jagla
authors.forename: Charles
authors.initials: C
authors.lastname: Buchen
authors.forename: Peter
authors.initials: P
authors.lastname: Stella
authors.forename: Simone
authors.initials: S
authors.lastname: Sanna-Cherchi
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Carnevali
authors.forename: Silvia
authors.initials: S
authors.lastname: Mattei
authors.forename: Achiropita
authors.initials: A
authors.lastname: Bovino
authors.forename: Lucia
authors.initials: L
authors.lastname: Argentiero
authors.forename: Andrea
authors.initials: A
authors.lastname: Magnano
authors.forename: Prasad
authors.initials: P
authors.lastname: Devarajan
authors.forename: Kai M
authors.initials: KM
authors.lastname: Schmidt-Ott
authors.forename: Landino
authors.initials: L
authors.lastname: Allegri
authors.forename: Paul
authors.initials: P
authors.lastname: Klotman
authors.forename: Vivette
authors.initials: V
authors.lastname: D'Agati
authors.forename: Ali G
authors.initials: AG
authors.lastname: Gharavi
authors.forename: Jonathan
authors.initials: J
authors.lastname: Barasch
mesh_terms: D016263:AIDS-Associated Nephropathy / Q000175:diagnosis / Q000652:urine*; D000209:Acute-Phase Proteins / Q000652:urine*; D000328:Adult; D000818:Animals; D005260:Female; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins / Q000652:urine*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000652:urine*
chemical_list: D000209:Acute-Phase Proteins; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins