title: Virtual terminator nucleotides for next-generation DNA sequencing.
abstract: We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
authors.affiliation: Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.
authors.forename: Jayson
authors.initials: J
authors.lastname: Bowers
authors.forename: Judith
authors.initials: J
authors.lastname: Mitchell
authors.forename: Eric
authors.initials: E
authors.lastname: Beer
authors.forename: Philip R
authors.initials: PR
authors.lastname: Buzby
authors.forename: Marie
authors.initials: M
authors.lastname: Causey
authors.forename: J William
authors.initials: JW
authors.lastname: Efcavitch
authors.forename: Mirna
authors.initials: M
authors.lastname: Jarosz
authors.forename: Edyta
authors.initials: E
authors.lastname: Krzymanska-Olejnik
authors.forename: Li
authors.initials: L
authors.lastname: Kung
authors.forename: Doron
authors.initials: D
authors.lastname: Lipson
authors.forename: Geoffrey M
authors.initials: GM
authors.lastname: Lowman
authors.forename: Subramanian
authors.initials: S
authors.lastname: Marappan
authors.forename: Peter
authors.initials: P
authors.lastname: McInerney
authors.forename: Adam
authors.initials: A
authors.lastname: Platt
authors.forename: Atanu
authors.initials: A
authors.lastname: Roy
authors.forename: Suhaib M
authors.initials: SM
authors.lastname: Siddiqi
authors.forename: Kathleen
authors.initials: K
authors.lastname: Steinmann
authors.forename: John F
authors.initials: JF
authors.lastname: Thompson
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D022202:Chromosomes, Artificial, Bacterial / Q000737:chemistry* / Q000235:genetics; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D004285:Dogs; D009711:Nucleotides / Q000737:chemistry* / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D009711:Nucleotides; D004247:DNA