title: NMR assignment of the nonstructural protein nsp3(1066-1181) from SARS-CoV.
abstract: Sequence-specific NMR assignments of the globular core comprising the residues 1066-1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [(13)C, (15)N]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the (13)C chemical shifts.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., MB-44, La Jolla, CA 92037, USA.
authors.forename: Pedro
authors.initials: P
authors.lastname: Serrano
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Johnson
authors.forename: Amarnath
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Bill
authors.initials: B
authors.lastname: Pedrini
authors.forename: Kurt
authors.initials: K
authors.lastname: Wüthrich
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009587:Nitrogen Isotopes / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011522:Protons; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D017361:Viral Nonstructural Proteins; C510959:Nsp3 protein, SARS-CoV; D012324:RNA-Dependent RNA Polymerase