title: First structural characterization of Burkholderia vietnamiensis lipooligosaccharide from cystic fibrosis-associated lung transplantation strains.
abstract: This is the first structural elucidation of the lipooligosaccharide (LOS) endotoxin isolated from Burkholderia vietnamiensis, a clinically important member of Burkholderia cepacia complex, a group of over 10 opportunistic species that are highly problematic in cystic fibrosis. We have characterized a novel LOS structure extracted from two clonal strains of B. vietnamiensis isolated from a cystic fibrosis patient who underwent lung transplantation. Strains were selected from the pretransplantation and post-transplantation periods and endotoxin was extracted. Subsequent analysis interestingly revealed identical oligosaccharidic sequences, but variation in lipid A moieties. Further, both LOS fractions were tested for their immunostimulatory activity on human myelomonocytic U937 cells and for signaling on an HEK293 cell line stably expressing both TLR 4 and MD-2. We observed an increase in lipid A acylation and a resultant increase in biological activity in bio-reporter assays of TNF-alpha secretion in the post-transplantation strain.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Napoli, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ieranò
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Luisa
authors.initials: L
authors.lastname: Sturiale
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
authors.forename: Clare
authors.initials: C
authors.lastname: Bryant
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Christine
authors.initials: C
authors.lastname: Aldridge
authors.forename: F Kate
authors.initials: FK
authors.lastname: Gould
authors.forename: Paul A
authors.initials: PA
authors.lastname: Corris
authors.forename: C M Anjam
authors.initials: CM
authors.lastname: Khan
authors.forename: Anthony
authors.initials: A
authors.lastname: De Soyza
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D019117:Burkholderia / Q000737:chemistry* / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016040:Lung Transplantation; D008969:Molecular Sequence Data
chemical_list: D008070:Lipopolysaccharides