title: Thermodynamic profile for urea photo-release from a N-(2-nitrobenzyl) caged urea compound.
abstract: Photoactivable bioactive molecules, often termed "caged" compounds, have attracted significant attention as useful tools for photo-regulating enzymatic activity. Here we examine the mechanism associated with photo-release of urea from a caged urea compound, N-(2-nitrobenzyl)urea, using photothermal beam deflection and time-resolved absorption spectroscopy. Photodissociation of the caged urea results in the prompt formation of an aci-nitro intermediate that decays to nitrosobenzaldehyde by releasing urea with the rate constant of 4.5x10(3) s(-1). Appearance of the aci-nitro intermediate is associated with a volume contraction of -13+/-1 mL mol(-1) and a negligible change in enthalpy (DeltaH=6+/-4 kcal mol(-1)). On the microsecond time-scale, the conversion of the aci-nitro intermediate and concomitant release of urea result in a volume expansion of 6+/-2 mL mol(-1) and a negative enthalpy change of -25+/-5 kcal mol(-1). No additional processes were observed on the timescale up to 100 ms suggesting that the breakdown of the aci-nitro intermediate is the rate-limiting step for urea photo-release. These results suggest a similar mechanism for caged urea photo release as determined previously for the caged ATP compound.
authors.affiliation: Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
authors.forename: Gangadhar
authors.initials: G
authors.lastname: Dhulipala
authors.forename: Marisa
authors.initials: M
authors.lastname: Rubio
authors.forename: Katja
authors.initials: K
authors.lastname: Michael
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Miksovská
mesh_terms: D007700:Kinetics; D010782:Photolysis; D013057:Spectrum Analysis; D013816:Thermodynamics; D014508:Urea / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: C544927:2-nitrobenzylurea; D014508:Urea