title: High-performance liquid-chromatographic separation of ofloxacin using a chiral stationary phase.
abstract: A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm x 4.6 mm, 5 microm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers.
authors.affiliation: Central Laboratory, Beijing Center for Disease Prevention & Control, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Sun
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Shao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002219:Carbamates / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D015242:Ofloxacin / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D002219:Carbamates; D002482:Cellulose; D015242:Ofloxacin