title: Cross-species amplification and polymorphism of microsatellite loci in the soybean aphid, Aphis glycines.
abstract: We tested the utility of 18 previously characterized Aphis spp. microsatellite loci for polymorphism and differentiation among populations of the soybean aphid, Aphis glycines. Loci were chosen from a closely related species (Aphis gossypii) and a more distantly related species (Aphis fabae). We found nine loci to be polymorphic among Korean and North American populations. Overall expected heterozygosity was moderate (average: 0.47; range: 0-1), although populations substantially differed in deviations from Hardy-Weinberg equilibrium. These loci will be valuable in characterizing population differentiation, migration and adaptation in an economically important pest of soybeans.
authors.affiliation: Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, USA. michel.70@osu.edu
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Michel
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jin Kyo
authors.initials: JK
authors.lastname: Jung
authors.forename: Sung-Taeg
authors.initials: ST
authors.lastname: Kang
authors.forename: M A Rouf
authors.initials: MA
authors.lastname: Mian
mesh_terms: D000818:Animals; D001042:Aphids / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005828:Genetics, Population; D007723:Korea; D018895:Microsatellite Repeats / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D013025:Glycine max / Q000469:parasitology*; D013045:Species Specificity; D014481:United States
chemical_list: D017931:DNA Primers