Genotoxic evaluation of Mikania laevigata extract on DNA damage caused by acute coal dust exposure.
In the present article, we report data on the possible antigenotoxic activity of Mikania laevigata extract (MLE) after acute intratracheal instillation of coal dust using the comet assay in peripheral blood, bone marrow, and liver cells and the micronucleus test in peripheral blood of Wistar rats. The animals were pretreated for 2 weeks with saline solution (groups 1 and 2) or MLE (100 mg/kg) (groups 3 and 4). On day 15, the animals were anesthetized with ketamine (80 mg/kg) and xylazine (20 mg/kg), and gross mineral coal dust (3 mg/0.3 mL saline) (groups 2 and 4) or saline solution (0.3 mL) (groups 1 and 3) was administered directly in the lung by intratracheal administration. Fifteen days after coal dust or saline instillation, the animals were sacrificed, and the femur, liver, and peripheral blood were removed. The results showed a general increase in the DNA damage values at 8 hours for all treatment groups, probably related to surgical procedures that had stressed the animals. Also, liver cells from rats treated with coal dust, pretreated or not with MLE, showed statistically higher comet assay values compared to the control group at 14 days after exposure. These results could be expected because the liver metabolizes a variety of organic compounds to more polar by-products. On the other hand, the micronucleus assay results did not show significant differences among groups. Therefore, our data do not support the antimutagenic activity of M. laevigata as a modulator of DNA damage after acute coal dust instillation.
Freitas
Tiago P
TP
Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil.
Heuser
Vanina D
VD
Tavares
Priscila
P
Leffa
Daniela D
DD
da Silva
Gabriela A
GA
Citadini-Zanette
Vanilde
V
Romão
Pedro R T
PR
Pinho
Ricardo A
RA
Streck
Emilio L
EL
Andrade
Vanessa M
VM
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001769:Blood / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D003031:Coal / Q000009:adverse effects*; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage / Q000187:drug effects*; D004391:Dust; D004781:Environmental Exposure / Q000009:adverse effects; D005269:Femur / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D015162:Micronucleus Tests / Q000379:methods; D035742:Mikania; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D016587:Antimutagenic Agents; D003031:Coal; D004391:Dust; D010936:Plant Extracts