title: Simultaneous use of direct and indirect diagnostic techniques in atypical respiratory infections from Chlamydophila pneumoniae and Mycoplasma pneumoniae.
abstract: In 2008, 50 samples (BAL), coming from hospital patients, with acute respiratory symptoms have been investigated using two real-time PCR methods: one assay for the single detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae DNA and one commercially available real-time duplex PCR assay for the detection of C. pneumoniae and M. pneumoniae DNA. Both techniques used here showed compliant results, with 100% concordance for detection of C. pneumoniae and 98% for detection of M. pneumoniae. The positive results obtained agreed with the clinical suspicion of such infections in some cases and with the presence of IgM specific for C. pneumoniae and M. pneumoniae in all cases of acute infection.
authors.affiliation: DESOS--Section of Microbiology, Policlinico S. Orsola, University of Bologna, 40138, Bologna, Italy. dott.pignanelli@libero.it
authors.forename: S
authors.initials: S
authors.lastname: Pignanelli
authors.forename: A
authors.initials: A
authors.lastname: Shurdhi
authors.forename: F
authors.initials: F
authors.lastname: Delucca
authors.forename: M
authors.initials: M
authors.lastname: Donati
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D017714:Community-Acquired Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018410:Pneumonia, Bacterial / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D011019:Pneumonia, Mycoplasma / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012698:Serologic Tests / Q000379:methods*
chemical_list: D004269:DNA, Bacterial