[Construction of integrative vector of Candida utilis].
OBJECTIVE
Xylanase is ahemicellulase. It can reduce xylan, which is the abundance resource on our earth. It is important in industries to obtain recombinant strains secreting xylanase to degrade hemicelluloses and produce xylose as desired products. We constructed an integrative vector of Candida utilis for xylanase expression.


METHODS
On the basis of plasmid pBR322, an integrative expression vector pGLR9K for Candida utilis was constructed that contained strong promoter of GAP, 18S rDNA sequence for homologous recombination and mutated L41 gene as CYH resistant marker. Recombinant expression vector pGLXR was constructed by adding xylanase gene to the pGLR9K and then was transferred into C. utilis.


RESULTS
Both intracellular and extracellular xylanase activities were detected in transgenic strains.


CONCLUSIONS
This system can be used for exogenous genes expression in transgenic C. utilis.
Yang
Honglan
H
Institute of Microbiology of Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China. YHLan-163@163.com
Wang
Wei
W
Bao
Huifang
H
Cui
Chunsheng
C
Hu
Shuang
S
D015139:Blotting, Southern; D002175:Candida / Q000235:genetics* / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics
D005656:Fungal Proteins; D043364:Endo-1,4-beta Xylanases