Screening of binding activity of Streptococcus pneumoniae, Streptococcus agalactiae and Streptococcus suis to berries and juices.
Antiadhesion therapy is a promising approach to the fight against pathogens. Antibiotic resistance and the lack of effective vaccines have increased the search for new methods to prevent infectious diseases. Previous studies have shown the antiadhesion activity of juice from cultivated cranberries (Vaccinium macrocarpon Ait.) against bacteria, especially E. coli. In this study, the binding of two streptococcal strains, Streptococcus pneumoniae and Streptococcus agalactiae, to molecular size fractions (FI, FII and FIII, <10 kDa, 10-100 kDa, and >100 kDa, respectively) of berries and berry and fruit juices from 12 plant species were studied using a microtiter well assay. For Streptococcus suis a hemagglutination inhibition assay was used. In general, binding activity was detected especially to wild cranberry (Vaccinium oxycoccos L.) and to other Vaccinium species. S. pneumoniae cells bound most to cranberry juice fraction FI and S. agalactiae cells to cranberry fraction FIII. Hemagglutination induced by S. suis was most effectively inhibited by cranberry fraction FII. NMR spectra of some characteristic active and non-active fractions were also measured. They indicate that fractions FII and FIII contained proanthocyanidins and/or other phenolic compounds. The results suggest Vaccinium berries as possible sources of antiadhesives against bacterial infections.
Toivanen
Marko
M
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Kuopio, Kuopio, Finland. marko.toivanen@uku.fi
Huttunen
Sanna
S
Duricová
Jana
J
Soininen
Pasi
P
Laatikainen
Reino
R
Loimaranta
Vuokko
V
Haataja
Sauli
S
Finne
Jukka
J
Lapinjoki
Seppo
S
Tikkanen-Kaukanen
Carina
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D001628:Beverages; D004912:Erythrocytes / Q000187:drug effects / Q000382:microbiology; D005638:Fruit / Q000737:chemistry*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D013292:Streptococcus agalactiae / Q000187:drug effects; D013296:Streptococcus pneumoniae / Q000187:drug effects; D017011:Streptococcus suis / Q000187:drug effects; D029796:Vaccinium / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D010936:Plant Extracts