title: Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic system.
abstract: The Epic system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.
authors.affiliation: Department of Neuroscience, High Throughput Biology Center and Johns Hopkins Ion Channel Center, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Wu
authors.forename: Shunyou
authors.initials: S
authors.lastname: Long
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Frutos
authors.forename: Maryna
authors.initials: M
authors.lastname: Eichelberger
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Fang
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D048948:14-3-3 Proteins; D010455:Peptides; D017868:Cyclic AMP-Dependent Protein Kinases