Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses.
Soluble egg antigens of the parasitic helminth Schistosoma mansoni (S. mansoni egg antigen [SEA]) induce strong Th2 responses both in vitro and in vivo. However, the specific molecules that prime the development of Th2 responses have not been identified. We report that omega-1, a glycoprotein which is secreted from S. mansoni eggs and present in SEA, is capable of conditioning human monocyte-derived dendritic cells in vitro to drive T helper 2 (Th2) polarization with similar characteristics as whole SEA. Furthermore, using IL-4 dual reporter mice, we show that both natural and recombinant omega-1 alone are sufficient to generate Th2 responses in vivo, even in the absence of IL-4R signaling. Finally, omega-1-depleted SEA displays an impaired capacity for Th2 priming in vitro, but not in vivo, suggesting the existence of additional factors within SEA that can compensate for the omega-1-mediated effects. Collectively, we identify omega-1, a single component of SEA, as a potent inducer of Th2 responses.
Everts
Bart
B
Department of Parasitology, Leiden University Medical Centre, Leiden 2333 ZA, The Netherlands.
Perona-Wright
Georgia
G
Smits
Hermelijn H
HH
Hokke
Cornelis H
CH
van der Ham
Alwin J
AJ
Fitzsimmons
Colin M
CM
Doenhoff
Michael J
MJ
van der Bosch
Jürgen
J
Mohrs
Katja
K
Haas
Helmut
H
Mohrs
Markus
M
Yazdanbakhsh
Maria
M
Schramm
Gabriele
G
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002454:Cell Differentiation; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D018345:Mice, Knockout; D010063:Ovum / Q000276:immunology; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D053662:Interleukin-4 Receptor alpha Subunit
D000947:Antigens, Helminth; D017077:Culture Media, Conditioned; D016207:Cytokines; D006023:Glycoproteins; D015801:Helminth Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C504002:Il4ra protein, mouse; D053662:Interleukin-4 Receptor alpha Subunit