Multiple stressor effects of methoprene, permethrin, and salinity on limb regeneration and molting in the mud fiddler crab (Uca pugnax).
Exposure to multiple stressors from natural and anthropogenic sources poses risk to sensitive crustacean growth and developmental processes. Applications of synthetic pyrethroids and insect growth regulators near shallow coastal waters may result in harmful mixture effects depending on the salinity regime. The potential for nonadditive effects of a permethrin (0.01 2 gg/L), methoprene (0.03-10 tg/L ), and salinity (10-40 ppt) exposure on male and female Uca pugnax limb regeneration and molting processes was evaluated by employing a central composite rotatable design with multifactorial regression. Crabs underwent single-limb autotomy followed by a molting challenge under I of 16 different mixture treatments. During the exposure (21-66 d), individual limb growth, major molt stage duration, abnormal limb regeneration, and respiration were monitored. At 6 d postmolt, changes in body mass, carapace width, and body condition factor were evaluated. Dorsal carapace tissue was collected, and protein and chitin were extracted to determine the composition of newly synthesized exoskeleton. The present results suggest chronic, low-dose exposures to multiple pesticide stressors cause less-than-additive effects on U. pugnax growth processes. Under increasing concentrations of methoprene and permethrin, males had more protein in their exoskeletons and less gain in body mass, carapace width, and body condition compared to females. Females exhibited less gain in carapace width than controls in response to methoprene and permethrin. Females also displayed elevated respiration rates at all stages of molt, suggesting a high metabolic rate. Divergent growth and fitness between the sexes over the long term could influence crustacean population resilience.
Stueckle
Todd A
TA
Biology Department, West Virginia University, Morgantown, West Virginia 26506, USA. tstueckl@mix.wvu.edu
Shock
Barbara
B
Foran
Christy M
CM
D000818:Animals; D003386:Brachyura / Q000187:drug effects* / Q000378:metabolism; D002686:Chitin / Q000032:analysis / Q000378:metabolism; D004781:Environmental Exposure; D005069:Evaluation Studies as Topic; D005121:Extremities / Q000502:physiology*; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D016014:Linear Models; D008297:Male; D008726:Methoprene / Q000633:toxicity*; D018978:Molting / Q000187:drug effects; D015999:Multivariate Analysis; D026023:Permethrin / Q000633:toxicity*; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D054712:Salinity; D012737:Sex Factors
D007306:Insecticides; D011506:Proteins; D002686:Chitin; D026023:Permethrin; D008726:Methoprene