title: Proteomic study of the effects of microcystin-LR on organelle and membrane proteins in medaka fish liver.
abstract: The microcystin-leucine-arginine toxin (MC-LR) is produced by cyanobacteria that sometimes bloom in water reservoirs. It targets the liver, thus posing potential health risks to human and animals. Microcystin inhibits the protein phosphatases PP1 and PP2A, leading to diverse cellular deregulation processes. A proteomic approach was applied to the medaka fish (Oryzias latipes) to obtain an overview of the effects of MC-LR on the liver. As membrane and organelle proteins are major structural and functional components of several cell signalling pathways, we decided to investigate here the membrane and organelle-enriched fractions from the livers of control and MC-LR treated medaka fish. Seventeen proteins were identified by proteomic analysis as being modulated in response to MC-LR treatment. This is the first time for eight of them to be reported as being involved in MC-LR effects: prohibitin, fumarylacetoacetase, protein disulfide isomerase A4 and A6, glucose regulated protein 78kDa, 40S ribosomal protein SA, cytochrome b5, and ATP synthase mitochondrial d subunit. These proteins are involved in protein maturation or in the response to oxidative stress highlighting the role of organelles in protein processing and the complex cooperation associated with oxidative stress.
authors.affiliation: CNRS, FRE 3206 Molécules de communication et adaptation des microorganismes, and MNHN, USM 505 Cyanobactéries, cyanotoxines et environnement, Département Régulations, développement et diversité moléculaire, Muséum national d'Histoire naturelle, Paris Cedex 05, France.
authors.forename: Mélodie
authors.initials: M
authors.lastname: Malécot
authors.forename: Karim
authors.initials: K
authors.lastname: Mezhoud
authors.forename: Arul
authors.initials: A
authors.lastname: Marie
authors.forename: Danièle
authors.initials: D
authors.lastname: Praseuth
authors.forename: Simone
authors.initials: S
authors.lastname: Puiseux-Dao
authors.forename: Marc
authors.initials: M
authors.lastname: Edery
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000633:toxicity*; D029941:Fish Proteins / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008387:Marine Toxins; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D052998:Microcystins / Q000633:toxicity*; D015388:Organelles / Q000187:drug effects / Q000378:metabolism; D009990:Oryzias / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004791:Enzyme Inhibitors; D029941:Fish Proteins; D008387:Marine Toxins; D008565:Membrane Proteins; D052998:Microcystins; D020543:Proteome; C057862:cyanoginosin LR