title: MicroRNAs with a nucleolar location.
abstract: There is increasing evidence that noncoding RNAs play a functional role in the nucleus. We previously reported that the microRNA (miRNA), miR-206, is concentrated in the nucleolus of rat myoblasts, as well as in the cytoplasm as expected. Here we have extended this finding. We show by cell/nuclear fractionation followed by microarray analysis that a number of miRNAs can be detected within the nucleolus of rat myoblasts, some of which are significantly concentrated there. Pronounced nucleolar localization is a specific phenomenon since other miRNAs are present at only very low levels in the nucleolus and occur at much higher levels in the nucleoplasm and/or the cytoplasm. We have further characterized a subset of these miRNAs using RT-qPCR and in situ hybridization, and the results suggest that some miRNAs are present in the nucleolus in precursor form while others are present as mature species. Furthermore, we have found that these miRNAs are clustered in specific sites within the nucleolus that correspond to the classical granular component. One of these miRNAs is completely homologous to a portion of a snoRNA, suggesting that it may be processed from it. In contrast, the other nucleolar-concentrated miRNAs do not show homology with any annotated rat snoRNAs and thus appear to be present in the nucleolus for other reasons, such as modification/processing, or to play roles in the late stages of ribosome biosynthesis or in nonribosomal functions that have recently been ascribed to the granular component of the nucleolus.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA. jritland@fhcrc.org
authors.forename: Joan C Ritland
authors.initials: JC
authors.lastname: Politz
authors.forename: Eric M
authors.initials: EM
authors.lastname: Hogan
authors.forename: Thoru
authors.initials: T
authors.lastname: Pederson
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism*; D002478:Cells, Cultured; D035683:MicroRNAs / Q000378:metabolism* / Q000502:physiology; D046228:Microarray Analysis; D032446:Myoblasts / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
chemical_list: D035683:MicroRNAs