The determination of glutamine with flow-injection chemiluminescence detection and mechanism study.
The main purpose of this study was to develop an inexpensive, simple, rapid and sensitive chemiluminescence (CL) method for the determination of glutamine (Gln) using a flow-injection (FI) system. Gln was found to strongly inhibit the CL signal of the luminol-H(2)O(2)-CuSO(4) system in Na(2)B(4)O(7) solution. A new FI-CL method was developed for the determination of Gln. Parameters affecting the reproducibility and CL detection were optimized systematically. Under the optimized conditions, the corresponding linear regression equation was established over the range of 5.0 x 10(-7) to 2.5 x 10(-6) mol/L with the detection limit of 1.8 x 10(-8) mol/L. The relative standard deviation was found to be 1.8% for 11 replicate determinations of 1.5 x 10(-6) mol/L Gln. The proposed method has been satisfactorily applied for the determination of Gln in real samples (Marzulene-s granules) with recoveries in the range of 98.7-108.6%. The minimum sampling rate was about 100 samples/h. The possible mechanism of this inhibitory CL was studied by fluorescence spectrophotometer and UV-vis spectrophotometer.
Liu
Yan-Ming
YM
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, People's Republic of China. liuym9518@sina.com
Liu
Zhuan-Li
ZL
Shi
Yan-Mei
YM
Tian
Wei
W
D019327:Copper Sulfate / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005973:Glutamine / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry
D005973:Glutamine; D008165:Luminol; D006861:Hydrogen Peroxide; D019327:Copper Sulfate