Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics.
The impact of chromosome architecture in the formation of chromosome aberrations is a recent finding of interphase directed molecular cytogenetic studies. Also positive correlation of translocation frequencies and spatial proximity of chromosomes was described. Thus, disease specific chromosomal translocations could be due to tissue specific genomic organization. However, no three-dimensional interphase fluorescence in situ hybridization (FISH) studies for the nuclear architecture of bone marrow (BM) cells have previously been done. In this study, BM of three secondary acute myelogenous leukemia (AML) cases with trisomy 8 and otherwise normal karyotype were evaluated. Bone marrow cells of one AML and one ALL (acute lymphoblastic leukemia) case, peripheral blood lymphocytes and human sperm, all of them with normal karyotype, served as controls. Multicolor banding (MCB) probes for chromosomes 8 and 21 were applied in suspension-FISH (S-FISH). Interestingly, in myeloid bone marrow cells chromosomes 8 (di- and trisomic) and 21 tended to co-localize with their homologue chromosome(s), rather than to be separated. Thus, the co-localization of chromosomes 8 and 21 might promote a translocation providing a selective advantage of t(8;21) cells in AML-M2. In summary, the concept that tissue specific spatial proximity of chromosomes leads to enhanced translocation frequencies was further supported.
Manvelyan
Marina
M
Jena University Hospital, Institute of Human Genetics and Anthropology, Jena, Germany.
Kempf
Philipp
P
Weise
Anja
A
Mrasek
Kristin
K
Heller
Anita
A
Lier
Amelie
A
Höffken
Klaus
K
Fricke
Hans-Jörg
HJ
Sayer
Herbert G
HG
Liehr
Thomas
T
Mkrtchyan
Hasmik
H
D000328:Adult; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002891:Chromosomes, Human, Pair 21 / Q000378:metabolism*; D002898:Chromosomes, Human, Pair 8 / Q000378:metabolism*; D020732:Cytogenetic Analysis / Q000379:methods*; D006801:Humans; D017403:In Situ Hybridization; D007399:Interphase; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism*