title: Vascular endothelial growth factor -2578 A/C, -460 T/C and +405 G/C polymorphisms in polycystic ovary syndrome.
abstract: OBJECTIVE
  Vascular endothelial growth factor (VEGF) may be involved in the physiological regulation of ovarian angiogenesis and pathogenesis of polycystic ovary syndrome (PCOS). VEGF -2578 A/C, -460 T/C and +405 G/C single nucleotide polymorphisms (SNPs) are known to be related to VEGF production.
  
  
  METHODS
  In order to investigate the possible association between VEGF gene and PCOS susceptibility, we analyzed genotype and allele distributions of above mentioned SNPs in 137 patients with PCOS and 155 healthy women. Differences in genotype distributions and allele frequencies in the cases and controls were compared for statistical significance using the chi(2)-test. Haplotype frequencies were estimated using a contingency chi(2)-test. Mann-Whitney U test was used for the statistics of the clinical and biochemical parameters.
  
  
  RESULTS
  No significant association between PCOS and the variant alleles of VEGF -2578 (OR: 0.91, 95% CI=0.65-1.26), -460 (OR: 0.78, 95% CI=0.56-1.08), and +405 (OR: 1.25, 95% CI=0.81-1.93) was observed. However, haplotype analysis demonstrated that the frequency of CTG haplotype, was higher among PCOS compared with controls (p=0.019) and that there is a strong linkage disequilibrium (D'=0.873, r(2)=0.752) between -2578 and -460 polymorphisms.
  
  
  CONCLUSIONS
  These preliminary results suggest that the -2578, -460 and +405 SNPs of VEGF gene are not significant risk factors for PCOS development alone. However, because of the high VEGF producer CTG haplotype was more frequent among the PCOS, we suppose that investigated polymorphisms--interacting with other genetic and environmental factors--could play a role in the development of PCOS.
authors.affiliation: Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, 34093 Capa, Istanbul, Turkey. pervinvural@yahoo.com
authors.forename: Pervin
authors.initials: P
authors.lastname: Vural
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Küskü-Kiraz
authors.forename: Semra
authors.initials: S
authors.lastname: Doğru-Abbasoğlu
authors.forename: Esra
authors.initials: E
authors.lastname: Cil
authors.forename: Berrin
authors.initials: B
authors.lastname: Karadağ
authors.forename: Cemil
authors.initials: C
authors.lastname: Akgül
authors.forename: Müjdat
authors.initials: M
authors.lastname: Uysal
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D055815:Young Adult
chemical_list: D042461:Vascular Endothelial Growth Factor A