Post-genomic insights into the plant polysaccharide degradation potential of  Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae.
The plant polysaccharide degradative potential of Aspergillus nidulans was analysed in detail and compared to that of Aspergillus niger and Aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. Manual verification indicated that 28.4% of the A. nidulans ORFs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method.While significant differences were found between the species in the numbers of ORFs assigned to the relevant CAZy families, no significant difference was observed in growth on polysaccharides. Growth differences were observed between the Aspergilli and Podospora anserina, which has a more different genomic potential for polysaccharide degradation, suggesting that large genomic differences are required to cause growth differences on polysaccharides. Differences were also detected between the Aspergilli in the presence of putative regulatory sequences in the promoters of the ORFs of this study and correlation of the presence of putative XlnR binding sites to induction by xylose was detected for A. niger. These data demonstrate differences at genome content, substrate specificity of the enzymes and gene regulation in these three Aspergilli, which likely reflect their individual adaptation to their natural biotope.
Coutinho
Pedro M
PM
AFMB-UMR 6098 CNRS/Universités Aix- Marseille I and II, Case 932, 163 Av de Luminy, 13288 Marseille Cedex 9, France.
Andersen
Mikael R
MR
Kolenova
Katarina
K
vanKuyk
Patricia A
PA
Benoit
Isabelle
I
Gruben
Birgit S
BS
Trejo-Aguilar
Blanca
B
Visser
Hans
H
van Solingen
Piet
P
Pakula
Tiina
T
Seiboth
Bernard
B
Battaglia
Evy
E
Aguilar-Osorio
Guillermo
G
de Jong
Jan F
JF
Ohm
Robin A
RA
Aguilar
Mariana
M
Henrissat
Bernard
B
Nielsen
Jens
J
Stålbrand
Henrik
H
de Vries
Ronald P
RP
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001234:Aspergillus niger / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019295:Computational Biology; D004798:Enzymes / Q000235:genetics*; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D016678:Genome; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011134:Polysaccharides / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013379:Substrate Specificity
D004798:Enzymes; D011134:Polysaccharides