Protective effect of augmenter of liver regeneration on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells.
BACKGROUND
Hydrogen peroxide, as other reactive oxygen species (ROS) produced during redox processes, induces lipid membrane peroxidation and protein degeneration causing cell apoptosis. ROS are recently considered as messengers in cell signalling processes, which, through reversible protein disulphide bridges formation, activate regulatory factors of cell proliferation and apoptosis. Disulphide bridges formation is catalysed by sulphydryl oxidase enzymes.


OBJECTIVE
The neuroprotective effect of ALR protein (Alrp), a sulphydryl oxidase enzyme, on H(2)O(2)-induced apoptosis in SH-SY5Y cells has been evaluated.


METHODS
Cell viability, flow cytometric evaluation of apoptotic cells, fluorescent changes of nuclear morphology, immunocytochemistry Alrp detection, Western blot evaluation of mitochondrial cyt c release and mitochondrial swelling were determined.


RESULTS
Alrp prevents the H(2)O(2)-induced cell viability loss, apoptotic cell death and mitochondrial swelling in SH-SY5Y cells in culture.


CONCLUSIONS
The data demonstrate that Alrp improves SH-SY5Y cells survival in H(2)O(2)-induced apoptosis. It is speculated that this effect could be related to the Alrp enzymatic activity.
Polimeno
Lorenzo
L
Section of Gastroenterology, Department of Emergency and Organ Transplantation (DETO), University of Bari, Bari, Italy.
Pesetti
Barbara
B
Lisowsky
Thomas
T
Iannone
Florenzo
F
Resta
Leonardo
L
Giorgio
Floriana
F
Mallamaci
Rosanna
R
Buttiglione
Maura
M
Santovito
Daniela
D
Vitiello
Francesco
F
Mancini
M Elisabetta
ME
Francavilla
Antonio
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003579:Cytochrome Reductases / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008933:Mitochondrial Swelling / Q000187:drug effects; D009447:Neuroblastoma / Q000201:enzymology / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D016877:Oxidants / Q000633:toxicity*; D050862:Oxidoreductases Acting on Sulfur Group Donors; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism
D016877:Oxidants; D011506:Proteins; D011994:Recombinant Proteins; C088825:GFER protein, rat; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D003579:Cytochrome Reductases; C500897:GFER protein, human; D050862:Oxidoreductases Acting on Sulfur Group Donors