title: Association of Rice gall dwarf virus with microtubules is necessary for viral release from cultured insect vector cells.
abstract: Vector insect cells infected with Rice gall dwarf virus, a member of the family Reoviridae, contained the virus-associated microtubules adjacent to the viroplasms, as revealed by transmission electron, electron tomographic, and confocal microscopy. The viroplasms, putative sites of viral replication, contained the nonstructural viral proteins Pns7 and Pns12, as well as core protein P5, of the virus. Microtubule-depolymerizing drugs suppressed the association of viral particles with microtubules and prevented the release of viruses from cells without significantly affecting viral multiplication. Thus, microtubules appear to mediate viral transport within and release of viruses from infected vector cells.
authors.affiliation: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
authors.forename: Tamaki
authors.initials: T
authors.lastname: Uehara-Ichiki
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miyazaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hibino
authors.forename: Kenji
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Omura
mesh_terms: D000818:Animals; D002213:Capsid / Q000378:metabolism; D002478:Cells, Cultured; D055032:Electron Microscope Tomography; D007181:Inclusion Bodies, Viral / Q000378:metabolism / Q000821:virology; D007303:Insect Vectors / Q000166:cytology* / Q000821:virology*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008870:Microtubules / Q000821:virology*; D012275:Oryza / Q000821:virology; D012087:Reoviridae / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D015678:Viral Structural Proteins / Q000378:metabolism; D014771:Virion / Q000378:metabolism; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins