EUROPLUS ANCA BIOCHIP mosaic: PR3 and MPO antigen microdots improve the laboratory diagnostics of ANCA-associated vasculitis.
BACKGROUND
The consensus on anti-neutrophil cytoplasmic antibody (ANCA) testing requires screening with indirect immunofluorescence (IIF) and confirmation in MPO- and PR3-ANCA specific assays. The EUROPLUS system combines in one incubation the conventional cell substrates with microdots of single antigens, i.e., MPO and PR3. We evaluated the diagnostic applicability of this new system for ANCA-associated vasculitis (AAV).


METHODS
To assess the diagnostic performance of the EUROPLUS Granulocyte Mosaic, sera from 249 AAV patients, 85 disease controls and 27 healthy controls were analysed. Results were compared with a reference multi-testing algorithm based on IIF with ethanol-fixed granulocytes, direct and capture ELISAs for both MPO- and PR3-ANCA.


RESULTS
Based on the reference multi-testing algorithm 123 AAV patients were defined as having PR3-ANCA and 68 AAV patients as having MPO-ANCA (diagnostic sensitivity: 76.7%). For the EUROPLUS MPO and PR3 microdots the diagnostic sensitivity was 77.1% in the same AAV cohort. The concordance between both methods for PR3- and MPO-ANCA was 96.8% and 99.2%, respectively. In the control cohort the diagnostic specificity was 99.1% for the multi-testing algorithm and 98.2% for the EUROPLUS microdots.


CONCLUSIONS
The combination of conventional cell substrates and single MPO and PR3 antigen microdots greatly facilitates the identification of ANCA reactivity clinically relevant for AAV. Since our results obtained after a single incubation in the EUROPLUS system are highly concordant with the reference multi-testing algorithm (based on IIF, direct and capture ELISAs) the EUROPLUS system is advocated as an efficient test system.
Damoiseaux
Jan
J
Department of Internal Medicine, Division of Clinical and Experimental Immunology, Maastricht University Medical Centre, The Netherlands. jan.damoiseaux@mumc.nl
Steller
Ulf
U
Buschtez
Michael
M
Vaessen
Mia
M
Rosemann
Anke
A
van Paassen
Pieter
P
Stöcker
Winfried
W
Fechner
Kai
K
Tervaert
Jan Willem Cohen
JW
D000465:Algorithms; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D001323:Autoantibodies / Q000097:blood; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000276:immunology; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis*; D014890:Granulomatosis with Polyangiitis / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay; D007377:Interleukin-3 / Q000032:analysis*; D053146:Myeloblastin / Q000032:analysis*; D040081:Protein Array Analysis; D011933:Reagent Kits, Diagnostic; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity
D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies; D007377:Interleukin-3; D011933:Reagent Kits, Diagnostic; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C404145:myelopoietin; D016179:Granulocyte Colony-Stimulating Factor; D053146:Myeloblastin