Subcellular localization of Trypanosoma cruzi arginine kinase.
Phosphoarginine is a cell energy buffer molecule synthesized by the enzyme arginine kinase. In Trypanosoma cruzi, the aetiological agent of Chagas' disease, 2 different isoforms were identified by data mining, but only 1 was expressed during the parasite life cycle. The digitonin extraction pattern of arginine kinase differed from those obtained for reservosomes, glycosomes and mitochondrial markers, and similar to the cytosolic marker. Immunofluorescence analysis revealed that although arginine kinase is localized mainly in unknown punctuated structures and also in the cytosol, it did not co-localize with any of the subcelular markers. This punctuated pattern has previously been observed in many cytosolic proteins of trypanosomatids. The knowledge of the subcellular localization of phosphagen kinases is a crucial issue to understand their physiological role in protozoan parasites.
Miranda
Mariana R
MR
Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas (IDIM), CONICET and Universidad de Buenos Aires, Buenos Aires, Argentina.
Bouvier
León A
LA
Canepa
Gaspar E
GE
Pereira
Claudio A
CA
D000818:Animals; D001122:Arginine Kinase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D019295:Computational Biology; D004072:Digitonin / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000378:metabolism; D008018:Life Cycle Stages; D013347:Subcellular Fractions / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D007527:Isoenzymes; D001122:Arginine Kinase; D004072:Digitonin