title: Influence of reduction in the elution times on HPLC glycohaemoglobin results.
abstract: OBJECTIVE
  To compare HPLC methods with short and long elution times for HbA1c measurement in blood.
  
  
  METHODS
  Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min).
  
  
  RESULTS
  Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means of the differences using Bland-Altman Plot were 0.02; -0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively.
  
  
  CONCLUSIONS
  Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.
authors.affiliation: Department of Clinical Biochemistry, Laboratory Buildings (4th Floor), Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville, Seville, Spain. antonio.leonj.sspa@juntadeandalucia.es
authors.forename: Antonio
authors.initials: A
authors.lastname: Leon-Justel
authors.forename: Jose D
authors.initials: JD
authors.lastname: Santotoribio
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Dominguez-Pascual
authors.forename: Ana L
authors.initials: AL
authors.lastname: Delgado
authors.forename: Carlos
authors.initials: C
authors.lastname: Macias
authors.forename: Maria T
authors.initials: MT
authors.lastname: Herrera
authors.forename: Alfonso
authors.initials: A
authors.lastname: Leal
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Mangas
authors.forename: Domingo
authors.initials: D
authors.lastname: Acosta
authors.forename: J M
authors.initials: JM
authors.lastname: Guerrero
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005319:Fetal Hemoglobin / Q000032:analysis; D006442:Glycated Hemoglobin / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis; D006801:Humans; D013997:Time Factors
chemical_list: D005319:Fetal Hemoglobin; D006442:Glycated Hemoglobin; D006451:Hemoglobin, Sickle; C517652:hemoglobin A1c protein, human