Lack of A-factor production induces the expression of nutrient scavenging and stress-related proteins in Streptomyces griseus.
The small gamma-butyrolactone A-factor is an important autoregulatory signaling molecule for the soil-inhabiting streptomycetes. Starvation is a major trigger for development, and nutrients are provided by degradation of the vegetative mycelium via a process of programmed cell death, reusing proteins, nucleic acids, and cell wall material. The A-factor regulon includes many extracellular hydrolases. Here we show via proteomics analysis that many nutrient-scavenging and stress-related proteins were overexpressed in an A-factor non-producing mutant of Streptomyces griseus B-2682. Transcript analysis showed that this is primarily due to differential transcription of the target genes during early development. The targets include proteins relating to nutrient stress and environmental stress and an orthologue of the Bacillus sporulation control protein Spo0M. The enhanced expression of these proteins underlines the stress that is generated by the absence of A-factor. Wild-type developmental gene expression was restored to the A-factor non-producing mutant by the signaling protein Factor C in line with our earlier observation that Factor C triggers A-factor production.
Birkó
Zsuzsanna
Z
Department of Human Genetics, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Swiatek
Magdalena
M
Szájli
Emília
E
Medzihradszky
Katalin F
KF
Vijgenboom
Erik
E
Penyige
András
A
Keseru
Judit
J
van Wezel
Gilles P
GP
Biró
Sándor
S
D015107:4-Butyrolactone / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis; D013305:Streptomyces griseus / Q000737:chemistry / Q000378:metabolism*; D013312:Stress, Physiological; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D020543:Proteome; D015107:4-Butyrolactone