Long term effects of high fat and sucrose diets on obesity and lymphocyte proliferation in mice.
OBJECTIVE
To clarify the effect of prolonged feeding of a high-fat and sucrose, and to clarify the effect of sucrose instead of other carbohydrate on obesity and immunity in C57BL/6J mice.


METHODS
We investigated the development of obesity and immune cell function in four groups of mice fed high-fat, high-fat plus high-sucrose, high-sucrose, and control diet for 7 months.


RESULTS
Mice fed high-fat and high-fat plus high-sucrose groups developed severe obesity. Body weight, adipose tissue weight, serum leptin, blood glucose, and insulin were significantly higher, while the level of serum soluble leptin receptor was significantly lower in mice fed high-fat and high-fat plus high-sucrose diets than in mice fed the control or high-sucrose diets. Splenocyte proliferation stimulated by T-cell mitogen (PHA, ConA, and anti-CD 3 antibody) and B-cell mitogen (LPS) was significantly lower in both obese, high-fat and high-fat plus high-sucrose groups than in control and high-sucrose groups. However, these parameters did not differ between high-fat and high-fat plus high-sucrose groups.


CONCLUSIONS
Long-term feeding of high-fat diet and high-fat plus high-sucrose diet similarly induced severe obesity in C57BL/6J mice. Not only T-cell, but also B-cell function may be impaired in mice made severely obese by the high-fat or high-fat plus high-sucrose diets.
Sato Mito
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Laboratory of Food and Nutritional Science, Department of Food, Azabu University, 1-17-71, Fuchinobe, Sagamihara-shi, Kanagawa 229-8501, Japan. satomito@azabu-u.ac.jp
Suzui
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M
Yoshino
H
H
Kaburagi
T
T
Sato
K
K
D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D019422:Dietary Sucrose / Q000494:pharmacology*; D005260:Female; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D009765:Obesity / Q000097:blood / Q000209:etiology* / Q000276:immunology; D009929:Organ Size / Q000187:drug effects; D054411:Receptors, Leptin / Q000097:blood; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology
D001786:Blood Glucose; D004041:Dietary Fats; D019422:Dietary Sucrose; D007328:Insulin; D020738:Leptin; D008934:Mitogens; D054411:Receptors, Leptin