Nocardioides humi sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.
Strain DCY24(T), a Gram-reaction-positive, aerobic, rod-shaped, motile bacterium, was isolated from soil of a ginseng field in South Korea. According to 16S rRNA gene sequence analysis, it was closely related to Nocardioides aromaticivorans DSM 15131(T) (95.1 % similarity), Nocardioides simplex KCTC 9106(T) (95.0 %), Nocardioides nitrophenolicus DSM 15529(T) (94.8 %) and Nocardioides kongjuensis DSM 19082(T) (94.7 %). Chemotaxonomic data revealed that strain DCY24(T) possessed MK-8(H(4)) as the predominant menaquinone, ll-2,6-diaminopimelic acid as the diagnostic diamino acid, phosphatidylglycerol and diphosphatidylglycerol as predominant polar lipids and iso-C(16 : 0), iso-C(17 : 0) and C(18 : 1)omega9c as predominant fatty acids. The DNA G+C content was 71.0 mol%. Based on evidence from this polyphasic study, strain DCY24(T) (=KCTC 19265(T) =LMG 24128(T)) should be classified as the type strain of a novel Nocardioides species, for which the name Nocardioides humi sp. nov. is proposed.
Kim
Myung Kyum
MK
Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Gyeonggi-do 449-701, South Korea.
Srinivasan
Sathiyaraj
S
Park
Min-Ju
MJ
Sathiyaraj
Gayathri
G
Kim
Yeon-Ju
YJ
Yang
Deok-Chun
DC
D000192:Actinomycetales / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D005894:Panax; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D001617:beta-Glucosidase / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D001617:beta-Glucosidase