title: Expression of proteins in the extracellular matrix of pulp tissue in human primary teeth during physiologic root resorption.
abstract: OBJECTIVE
  To analyze the expression of tenascin, fibronectin, collagens I and III, osteonectin, and bone morphogenetic protein 4 (BMP4) in the extracellular matrix of pulp tissue in primary teeth during physiologic root resorption.
  
  
  METHODS
  Eighteen teeth were decalcified and equally distributed into 3 groups (group I, teeth with two-thirds root length; group II, teeth with one-third root length; and group III, teeth lacking the root).
  
  
  RESULTS
  Immunohistochemical analysis showed that all the proteins were expressed. Tenascin, collagen I, and osteonectin showed strong and broad reactivity in group I, with weaker and rare reactivity in groups II and III. The expression of fibronectin, collagen III, and BMP4 did not vary with root resorption phase.
  
  
  CONCLUSIONS
  The expression of tenascin, collagen I, and osteonectin was reduced in the extracellular matrix and odontoblasts during root resorption. This fact may be related to the decreasing pulp response to damage and treatment during the progression of root resorption.
authors.affiliation: São Leopoldo Mandic Dental Research, School of Dentistry, University of São Paulo, São Paulo, Brazil. bonecker@usp.br
authors.forename: Marcelo
authors.initials: M
authors.lastname: Bönecker
authors.forename: Andrea
authors.initials: A
authors.lastname: Mantesso
authors.forename: Ney Soares
authors.initials: NS
authors.lastname: de Araújo
authors.forename: Vera Cavalcanti
authors.initials: VC
authors.lastname: Araújo
mesh_terms: D024042:Collagen Type I / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D015536:Down-Regulation; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009804:Odontoblasts / Q000378:metabolism; D015676:Osteonectin / Q000096:biosynthesis; D012391:Root Resorption / Q000378:metabolism*; D019063:Tenascin / Q000096:biosynthesis; D014080:Tooth Exfoliation; D014094:Tooth, Deciduous / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D015676:Osteonectin; D019063:Tenascin