title: Enhanced cell adhesion to silicone implant material through plasma surface modification.
abstract: Silicone implant material is widely used in the field of plastic surgery. Despite its benefits the lack of biocompatibility this material still represents a major problem. Due to the surface characteristics of silicone, protein adsorption and cell adhesion on this polymeric material is rather low. The aim of this study was to create a stable collagen I surface coating on silicone implants via glow-discharge plasma treatment in order to enhance cell affinity and biocompatibility of the material. Non-plasma treated, collagen coated and conventional silicone samples (non-plasma treated, non-coated) served as controls. After plasma treatment the change of surface free energy was evaluated by drop-shape analysis. The quality of the collagen coating was analysed by electron microscopy and Time-Of-Flight Secondary Ion Mass Spectrometry. For biocompatibility tests mouse fibroblasts 3T3 were cultivated on the different silicone surfaces and stained with calcein-AM and propidium iodine to evaluate cell viability and adherence. Analysis of the different surfaces revealed a significant increase in surface free energy after plasma pre-treatment. As a consequence, collagen coating could only be achieved on the plasma activated silicone samples. The in vitro tests showed that the collagen coating led to a significant increase in cell adhesion and cell viability.
authors.affiliation: Department of Plastic Surgery, BG Trauma Center Bergmannsheil, University Hospital, Bochum, Germany. joerg.hauser@rub.de
authors.forename: J
authors.initials: J
authors.lastname: Hauser
authors.forename: J
authors.initials: J
authors.lastname: Zietlow
authors.forename: M
authors.initials: M
authors.lastname: Köller
authors.forename: S A
authors.initials: SA
authors.lastname: Esenwein
authors.forename: H
authors.initials: H
authors.lastname: Halfmann
authors.forename: P
authors.initials: P
authors.lastname: Awakowicz
authors.forename: H U
authors.initials: HU
authors.lastname: Steinau
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D051381:Rats; D012828:Silicones; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012828:Silicones