General peroxidase activity of G-quadruplex-hemin complexes and its application in ligand screening.
DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high peroxidase activity of hemin complexed with intramolecular parallel G-quadruplex-forming sequences in gene promoters (such as c-Myc, VEGF, c-Kit21, HIF-1alpha, and RET) may imply a potential mechanism of hemin-mediated cellular injury. This peroxidase activity has also been demonstrated to be applicable for screening G-quadruplex ligands (potential anticancer reagents) using colorimetric and visual detection strategies.
Cheng
Xiaohong
X
Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Liu
Xiangjun
X
Bing
Tao
T
Cao
Zehui
Z
Shangguan
Dihua
D
D002384:Catalysis; D002942:Circular Dichroism; D054856:G-Quadruplexes / Q000187:drug effects; D006147:Guanine / Q000378:metabolism; D006427:Hemin / Q000009:adverse effects* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008165:Luminol / Q000737:chemistry / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000009:adverse effects / Q000378:metabolism
D008024:Ligands; D017382:Reactive Oxygen Species; D008165:Luminol; D006147:Guanine; D006427:Hemin; D006861:Hydrogen Peroxide; D009195:Peroxidase