Backbone assignment of osteopontin, a cytokine and cell attachment protein implicated in tumorigenesis.
OPN is an RGD-containing protein overexpressed in cells transformed by v-myc and v-mil(raf) oncogenes. Here we report the resonance assignment of recombinant quail OPN and provide NMR evidence that quail OPN is an intrinsically unstructured protein in solution.
Schedlbauer
Andreas
A
Department of Biomolecular Structural Chemistry, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter Campus 5, 1030 Vienna, Austria.
Ozdowy
Przemyslaw
P
Kontaxis
Georg
G
Hartl
Markus
M
Bister
Klaus
K
Konrat
Robert
R
D000595:Amino Acid Sequence; D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D016207:Cytokines / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009369:Neoplasms / Q000378:metabolism; D009587:Nitrogen Isotopes / Q000737:chemistry; D053495:Osteopontin / Q000737:chemistry*; D011522:Protons; D011784:Quail / Q000378:metabolism*
D002247:Carbon Isotopes; D015815:Cell Adhesion Molecules; D016207:Cytokines; D009587:Nitrogen Isotopes; D011522:Protons; D053495:Osteopontin