Differential solubility of prions is associated in manifold phenotypes.
The main feature of prion diseases is the accumulation of infectious proteins (PrP(Sc)). Since PrP(Sc) results from conversion of cellular prion proteins (PrP(C)), differential expressed PrP(C) types may play an important role in the formation and conversion efficiency to specific PrP(Sc) forms. However, little is known about the PrP(C) expression, regulation and differentiation. Here, we demonstrate a new type of differentiation of overlapping PrP(C) isoforms in brain homogenates using differential SDS solubility. Low and highly soluble PrP(C) were detected along with various types of protein which are present in the brain of non-infected humans, sheep and cattle. Our findings provide evidence for the existence of several overlapping PrP(C) proteins exhibiting distinct glycotypes. The selection of defined PrP(C) types offers new possibilities for identifying highly efficient converting proteins and provides the potential for disease control.
Kuczius
Thorsten
T
Institute for Hygiene, Westfälische Wilhelms-University Münster and University Hospital Münster, Robert Koch-Strasse 41, 48149 Münster, Germany. tkuczius@uni-muenster.de
Karch
Helge
H
Groschup
Martin H
MH
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D001923:Brain Chemistry; D002417:Cattle; D003902:Detergents / Q000737:chemistry; D006801:Humans; D010641:Phenotype; D018621:PrPC Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018620:PrPSc Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017096:Prion Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020033:Protein Isoforms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012756:Sheep; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility
D003902:Detergents; D018621:PrPC Proteins; D018620:PrPSc Proteins; D020033:Protein Isoforms; D012967:Sodium Dodecyl Sulfate