title: [Preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method].
abstract: OBJECTIVE
  To evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms.
  
  
  METHODS
  Suspensions of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum with same density were prepared and cultured respectively at liquid BHI medium. Then the growth quantity was measured periodically through turbidimetry and the growth curves of the inoculated bacteria were completed. The culture solutions of stable growth phase were sampled and characterized by 1H-nuclear magnetic resonance 1H-NMR). The data of 1H-NMR spectroscope results were analyzed by principal components analysis (PCA).
  
  
  RESULTS
  The PCA showed the obvious clustering phenomena and the points of three groups differentially centralized to three clusters. Therefore, the NMR-based metabonomics profiles could discriminate the three different kinds of bacteria.
  
  
  CONCLUSIONS
  The metabonomics is a potential classable method to identify the oral pathogenic bacteria.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Wei-Xi
authors.initials: WX
authors.lastname: Lu
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Wu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Xiao
authors.forename: Ming-Yun
authors.initials: MY
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Xiong
authors.forename: Xiang-Ming
authors.initials: XM
authors.lastname: Jia
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Xiao
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zhu
authors.forename: Qi-Mei
authors.initials: QM
authors.lastname: Gong
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans; D001419:Bacteria; D016967:Fusobacterium nucleatum; D055432:Metabolomics; D009055:Mouth / Q000382:microbiology; D016966:Porphyromonas gingivalis; D018713:Prevotella intermedia