title: 1H and 15N resonance assignment of the second fibronectin type III module of the neural cell adhesion molecule.
abstract: We report here the NMR assignment of the second fibronectin type III module of the neural cell adhesion molecule (NCAM). This module has previously been shown to interact with the fibroblast growth factor receptor (FGFR), and the FGFR-binding site was mapped by NMR to the FG-loop region of the module. The FG-loop region also contains a putative nucleotide-binding motif, which was shown by NMR to interact with ATP. Furthermore, ATP was demonstrated to inhibit binding of the second F3 module of NCAM to FGFR.
authors.affiliation: Protein Laboratory, Institute of Molecular Pathology, Blegdamsvej 3, Copenhagen, Denmark. vkis@novonordisk.com
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Kiselyov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Berezin
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Bock
authors.forename: Flemming M
authors.initials: FM
authors.lastname: Poulsen
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry*; D009587:Nitrogen Isotopes / Q000737:chemistry; D017434:Protein Structure, Tertiary
chemical_list: D002247:Carbon Isotopes; D005353:Fibronectins; D019006:Neural Cell Adhesion Molecules; D009587:Nitrogen Isotopes; C501364:fibronectin type III like peptide, human