title: Intraarterially delivered human umbilical cord blood-derived mesenchymal stem cells in canine cerebral ischemia.
abstract: The present study examined the effects of human umbilical cord blood-derived mesenchymal stem cells (HUCB-derived MSCs) delivered through the basilar artery in a canine thromboembolic brain ischemia model. Cerebral ischemia was induced through occlusion of the middle cerebral artery by injecting thrombus emboli into 10 beagles. In the HUCBC group (n = 5), 1 x 10(6) HUCB-derived MSCs were transplanted through the basilar artery 1 day after ischemic induction using an endovascular interventional approach. In the control group (n = 5), phosphate-buffered saline (PBS) was injected in the same manner in as the HUCBC group. Upon neurobehavioral examination, earlier recovery was observed in the HUCBC group. The HUCBC group showed a decrease in the infarction volume at 1 week after cerebral ischemic induction, whereas the control group showed an increase in the infarction volume at 1 week, by magnetic resonance image analysis. Transplanted cells had differentiated into neurons and astrocytes and were observed in and around endothelial cells that were positive for von Willebrand factor (vWF). HUCB-derived MSCs expressed neuroprotective factors, such as brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF), at 4 weeks after the transplantation. The transplanted cells demonstrated their efficacy by reducing the infarction lesion volume and through earlier recovery from the neurological deficit. These results suggest that intraarterial transplantation of HUCB-derived MSCs could be useful in clinical treatment of cerebral ischemia.
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Dai-Jung
authors.initials: DJ
authors.lastname: Chung
authors.forename: Chi-Bong
authors.initials: CB
authors.lastname: Choi
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Kang
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Soo-Han
authors.initials: SH
authors.lastname: Hwang
authors.forename: Hoon
authors.initials: H
authors.lastname: Han
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Choe
authors.forename: Soo-Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Hwi-Yool
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D036101:Cord Blood Stem Cell Transplantation; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006801:Humans; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D020127:Recovery of Function; D018709:Statistics, Nonparametric; D013997:Time Factors; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor