title: Synthesis and characterization of a cellular membrane affinity chromatography column containing histamine 1 and P2Y(1) receptors: a multiple G-protein coupled receptor column.
abstract: A cellular membrane affinity chromatography (CMAC) column has been created using cellular membrane fragments from a 1321N1 cell line stably transfected with the P2Y(1) receptor. The CMAC(1321N1(P2Y1)) column contained functional P2Y(1) and histamine 1 receptors, which independently bound receptor-specific ligands. The data obtained with the CMAC(1321N1(P2Y1)) column demonstrate that multiple-G-protein coupled receptor (GPCR) columns can be developed and used to probe interactions with the immobilized receptors and that endogenously expressed GPCRs can be used to create CMAC columns. The results also establish that the histamine 1 receptor can be immobilized with retention of ligand-specific binding.
authors.affiliation: National Institute on Aging, National Institutes of Health, Gerontology Research Center, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, United States. moaddelru@mail.nih.gov
authors.forename: Ruin
authors.initials: R
authors.lastname: Moaddel
authors.forename: Harrison K
authors.initials: HK
authors.lastname: Musyimi
authors.forename: Mitesh
authors.initials: M
authors.lastname: Sanghvi
authors.forename: Charlene
authors.initials: C
authors.lastname: Bashore
authors.forename: Chester R
authors.initials: CR
authors.lastname: Frazier
authors.forename: Mohammad
authors.initials: M
authors.lastname: Khadeer
authors.forename: Prateek
authors.initials: P
authors.lastname: Bhatia
authors.forename: Irving W
authors.initials: IW
authors.lastname: Wainer
mesh_terms: D001254:Astrocytoma / Q000473:pathology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D006801:Humans; D007700:Kinetics; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D011968:Receptors, Histamine / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D014162:Transfection
chemical_list: D043562:Receptors, G-Protein-Coupled; D011968:Receptors, Histamine; D018048:Receptors, Purinergic P2