title: Association of the DNMT3B polymorphism with colorectal adenomatous polyps and adenocarcinoma.
abstract: DNMT3B is an important enzyme to modulate the methylation status in mammalian cells. The aim of this study is to investigate the correlation of the DNMT3B G39179T polymorphism with the susceptibilities of colorectal adenomatous polyps and adenocarcinoma. This case-control study included 146 colorectal adenomatous polyps, 170 colorectal adenocarcinoma patients, and 157 normal controls. DNMT3B polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism analysis. Family history of colorectal cancer significantly increases the risk of developing colorectal adenomatous polyps and adenocarcinoma. The genotype frequency of DNMT3B polymorphism (T/T and G/T + G/G) in adenocarcinoma patients was significantly different from that in controls (P value = 0.01). Compared with DNMT3B T/T genotype, the G allelotype (G/T + G/G genotype) had lower risk to develop colorectal adenocarcinoma (OR = 0.50, 95% CI = 0.29-0.87); while there was no significant difference between the colorectal adenomatous polyps patients and controls (OR = 0.63, 95% CI = 0.37-1.09), although descending tendency could be found in this polyps group. In the stratification analysis, a significant association was confined to subgroups of age < 55 (OR = 0.31, 95% CI = 0.12-0.84) and males (OR = 0.35, 95% CI = 0.17-0.71). Meanwhile, combined G/T + G/G genotypes were found to have a lower risk in non-drinkers to develop both colorectal adenomatous polyps and adenocarcinoma (OR = 0.54, 95% CI = 0.31-0.96 and OR = 0.48, 95% CI = 0.27-0.84, respectively). This study also showed a distinct difference in the distribution of DNMT3B G39179T SNP in different ethnics. DNMT3B G39179T SNP may be a potential genetic susceptibility factor for adenocarcinoma of the colon, especially in younger Chinese Han non-drinker men.
authors.affiliation: Department of Endoscopy, The 4th Affiliated Hospital, Hebei Medical University, Hebei, People's Republic of China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Guo
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhang
authors.forename: Mingli
authors.initials: M
authors.lastname: Wu
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Limian
authors.initials: L
authors.lastname: Er
authors.forename: Shunping
authors.initials: S
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Weifang
authors.initials: W
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Xu
authors.forename: Shijie
authors.initials: S
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics*; D018256:Adenomatous Polyps / Q000201:enzymology / Q000235:genetics*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D003710:Demography; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B