title: Use of a T cell interferon-gamma release assay to evaluate tuberculosis risk in newly qualified physicians in Singapore healthcare institutions.
abstract: BACKGROUND
  Surveillance for latent tuberculosis in high-risk groups such as healthcare workers is limited by the nonspecificity of the tuberculin skin test (TST) in BCG-vaccinated individuals. The Mycobacterium tuberculosis antigen-specific interferon-gamma release assays (IGRAs) show promise for more accurate latent tuberculosis detection in such groups.
  
  
  OBJECTIVE
  To compare the utility of an IGRA, the T-SPOT.TB assay, with that of the TST in healthcare workers with a high rate of BCG vaccination.
  
  
  METHODS
  Two hundred seven medical students from 2 consecutive cohorts underwent the T-SPOT.TB test and the TST in their final year of study. Subjects with negative baseline test results underwent repeat testing after working for 1 year as junior physicians in Singapore's public hospitals.
  
  
  RESULTS
  The baseline TST result was an induration 10 mm or greater in diameter in 177 of the 205 students who returned to have their TST results evaluated (86.3%), while the baseline T-SPOT.TB assay result was positive in 9 (4.3%) of the students. Repeat T-SPOT.TB testing in 182 baseline-negative subjects showed conversion in 9 (4.9%). A repeat TST in 18 subjects with baseline-negative TST results did not reveal any TST result conversion.
  
  
  CONCLUSIONS
  The high rate of positive baseline TST results in our BCG-vaccinated healthcare workers renders the TST unsuitable as a surveillance tool in this tuberculosis risk group. Use of an IGRA has enabled the detection and treatment of latent tuberculosis in this group. Our T-SPOT.TB conversion rate highlights the need for greater tuberculosis awareness and improved infection control practices in our healthcare institutions.
authors.affiliation: Tuberculosis Control Unit, Singapore. cynthia_chee@ttsh.com.sg
authors.forename: C B E
authors.initials: CB
authors.lastname: Chee
authors.forename: L K Y
authors.initials: LK
authors.lastname: Lim
authors.forename: T M
authors.initials: TM
authors.lastname: Barkham
authors.forename: D R
authors.initials: DR
authors.lastname: Koh
authors.forename: S O
authors.initials: SO
authors.lastname: Lam
authors.forename: L
authors.initials: L
authors.lastname: Shen
authors.forename: Y T
authors.initials: YT
authors.lastname: Wang
mesh_terms: D000328:Adult; D001500:BCG Vaccine / Q000008:administration & dosage; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010564:Personnel, Hospital; D012307:Risk Factors; D012846:Singapore; D013337:Students, Medical; D013601:T-Lymphocytes / Q000276:immunology*; D014374:Tuberculin Test / Q000379:methods; D014376:Tuberculosis / Q000175:diagnosis* / Q000517:prevention & control; D055815:Young Adult
chemical_list: D001500:BCG Vaccine; D007371:Interferon-gamma