title: Interactions of typical and atypical enteropathogenic Escherichia coli strains with the calf intestinal mucosa ex vivo.
abstract: Enteropathogenic Escherichia coli (EPEC) can be found in healthy and diarrheic cattle; however, little is known about the role of attaching and effacing (A/E) lesion formation in colonization of bovine intestinal mucosa by such strains. We show that typical and atypical EPEC induce A/E lesions on calf intestinal explants independently of Tir tyrosine phosphorylation and TccP. Our data support the existence of conserved Tir- and TccP-independent mechanisms of A/E lesion formation in a range of hosts and reinforce the zoonotic potential of EPEC in cattle.
authors.affiliation: Centre for Molecular Microbiology and Infection, Division of Cell & Molecular Biology, Flowers Building, Imperial College London, London SW72AZ, United Kingdom.
authors.forename: Francis
authors.initials: F
authors.lastname: Girard
authors.forename: Francis
authors.initials: F
authors.lastname: Dziva
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Gad
authors.initials: G
authors.lastname: Frankel
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D054308:Enteropathogenic Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology; D010766:Phosphorylation
chemical_list: D029968:Escherichia coli Proteins; C505370:tccP protein, E coli