[Identification of mycobacteria by high-performance liquid chromatography].
OBJECTIVE
To compare results of mycobacteria identification by bacteriologic methods as well as by high-performance liquid chromatography (HPLC).


METHODS
Two hundred and eighty strains of mycobacteria isolated from respiratory specimens and identified by bacteriologic methods and HPLC were studied.


RESULTS
It was established that results of HLPC use were highly correlated with results of microbiologic methods of mycobacteria identification: for identification of M. tuberculosis complex the correlation was 97.0%, for nontuberculous (NTM) slowly growing mycobacteria--95.3%, for quickly growing NTM--96.2% (overall--96.1%). Results of identification of mycobacteria by HPLC were ready in significantly shorter time-frame (during 24 hours).


CONCLUSIONS
HLPC method could be recommended for identification of mycobacteria in bacteriologic reference laboratories.
Makarova
M V
MV
Krasnova
M A
MA
Moroz
A M
AM
D002851:Chromatography, High Pressure Liquid; D003937:Diagnosis, Differential; D006801:Humans; D009161:Mycobacterium / Q000145:classification / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity