The crustacean parasites Ellobiopsis Caullery, 1910 and Thalassomyces Niezabitowski, 1913 form a monophyletic divergent clade within the Alveolata.
The Ellobiopsidae are enigmatic parasites of crustaceans that have been grouped together exclusively on the basis of morphological similarities. Ultrastructural studies have revealed their affiliation within the alveolates, which was confirmed by the phylogenetic analysis of the ribosomal RNA gene (SSU rDNA) sequences of two species of Thalassomyces Niezabitowski, 1913. However, their precise systematic position within this group remains unresolved, since they could not be definitively allied with any particular alveolate group. To better determine the systematic position of ellobiopsids by molecular phylogeny, we sequenced the SSU rDNA from the type-species of the Ellobiopsidae, Ellobiopsis chattoni Caullery, 1910. We found E. chattoni infecting various copepod hosts, Acartia clausi Giesbrecht, Centropages typicus Kröyer and Clausocalanus sp., in the Bay of Marseille, NW Mediterranean Sea, which allowed us to study several stages of the parasite development. A single unicellular multinucleate specimen provided two different sequences of the SSU rDNA gene, indicating the existence of polymorphism at this locus within single individuals. Ellobiopsis Caullery, 1910 and Thalassomyces formed a very divergent and well-supported clade in phylogenetic analyses. This clade appears to be more closely related to the dinoflagellates (including the Syndiniales/Marine Alveolate Group II and the Dinokaryota) and Marine Alveolate Group I than to the other alveolates (Ciliophora, Perkinsozoa and Apicomplexa).
Gómez
Fernando
F
Observatoire Océanologique de Banyuls sur Mer, Université Pierre et Marie Curie, CNRS-INSU UMR 7621, BP 44, 66651 Banyuls sur Mer, France. fernando.gomez@fitoplancton.com
López-García
Purificación
P
Nowaczyk
Antoine
A
Moreira
David
D
D000818:Animals; D016000:Cluster Analysis; D003445:Crustacea / Q000469:parasitology*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008522:Mediterranean Sea; D008853:Microscopy / Q000379:methods; D008969:Molecular Sequence Data; D010271:Parasites / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S