title: Epsilon haemoglobin specific antibodies with applications in noninvasive prenatal diagnosis.
abstract: Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial. Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures. Identification of NRBCs in blood samples would be possible provided that specific antibodies are available. Here we have isolated recombinant antibodies using phage display. From the panel of antibody fragments specifically recognising epsilon-Hb, one was chosen for further characterization, DAb1. DAb1 binds to epsilon-Hb both in Western blots and immunocytochemistry. Several epsilon-Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS). To evaluate the sensitivity of the method, K562 cells (which express epsilon-Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.
authors.affiliation: Department of Molecular Biology, University of Aarhus, Denmark.
authors.forename: Morten Draeby
authors.initials: MD
authors.lastname: Sørensen
authors.forename: Regina
authors.initials: R
authors.lastname: Gonzalez Dosal
authors.forename: Kim Bak
authors.initials: KB
authors.lastname: Jensen
authors.forename: Britta
authors.initials: B
authors.lastname: Christensen
authors.forename: Steen
authors.initials: S
authors.lastname: Kølvraa
authors.forename: Uffe Birk
authors.initials: UB
authors.lastname: Jensen
authors.forename: Peter
authors.initials: P
authors.lastname: Kristensen
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D004900:Erythroblasts / Q000166:cytology* / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020014:K562 Cells; D019151:Peptide Library; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012680:Sensitivity and Specificity; D055547:epsilon-Globins / Q000032:analysis / Q000276:immunology*
chemical_list: D000906:Antibodies; D019151:Peptide Library; D011994:Recombinant Proteins; D055547:epsilon-Globins