Simple and sensitive HPLC method for determination of amrubicin and amrubicinol in human plasma: application to a clinical pharmacokinetic study.
A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for determination of amrubicin and its metabolite amrubicinol in human plasma. After protein precipitation with methanol without evaporation procedure, large volume samples were injected and separated by two monolithic columns with a guard column. The mobile phase consisted of tetrahydrofuran-dioxane-water (containing 2.3 mM acetic acid and 4 mM sodium 1-octanesulfonate; 2:6:15, v/v/v). Wavelengths of fluorescence detection were set at 480 nm for excitation and 550 nm for detection. Under these conditions, linearity was confirmed in the 2.5-5000 ng/mL concentration range of both compounds. The intra- and inter-day precision and intra- and inter-day accuracy for both compounds were less than 10%. The method was successfully applied to a clinical pharmacokinetic study of amrubicin and amrubicinol in cancer patients.
Ando
Reiko
R
National Cancer Center Hospital, Chuo-ku, Tokyo, Japan. reando@ncc.go.jp
Makino
Yoshinori
Y
Tamura
Tomohide
T
Yamamoto
Noboru
N
Nishigaki
Rena
R
Kimura
Takehiro
T
Yokote
Nobuaki
N
Yamamoto
Hiroshi
H
D018943:Anthracyclines / Q000097:blood*; D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy; D011446:Prospective Studies; D012680:Sensitivity and Specificity
D018943:Anthracyclines; D000970:Antineoplastic Agents; C116173:amrubicinol; C055866:amrubicin