Factorial design for multivariate optimization of preconcentration system for spectrophotometric phosphorus determination.
The present paper proposes a preconcentration procedure for phosphorus determination by using ultraviolet-visible spectrophotometer. It is based on the formation of phosphomolybdate and its reduction to molybdenum blue. Phosphorus extraction as phosphomolybdenum blue complex was performed onto Amberlite XAD-4. The optimization step was carried out using two-level full factorial design. Three variables (resin amount, sample volume, flow rate) were regarded as factors in the optimization. The relative standard deviation was 2% at 0.08 microg mL(-1). The limit of detection was found to be 2.23 microg L(-1) (N=15). The proposed solid-phase extraction procedure was applied to phosphorus in some fruit leaves, natural waters, and a standard reference material (SRM 1515 apple leaves).
Divrikli
Umit
U
Pamukkale University, Faculty of Arts and Science, Department of Chemistry, 20020 Denizli, Turkey. udivrikli@pamukkale.edu.tr
Akdogan
Abdullah
A
Soylak
Mustafa
M
Elci
Latif
L
D005618:Fresh Water / Q000032:analysis; D005638:Fruit; D008982:Molybdenum / Q000737:chemistry; D015999:Multivariate Analysis; D010756:Phosphoric Acids / Q000737:chemistry; D010758:Phosphorus / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D012015:Reference Standards; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D010756:Phosphoric Acids; D010758:Phosphorus; C017541:molybdenum blue; D008982:Molybdenum; C003125:phosphomolybdic acid