[Rapid establishment of suspension cell lines in japonica rice].
With Jingkang No.5 (PiA), calli of the PiA induced for 10-15 days were transferred into amino acid liquid culture medium, to establish excellent rice suspension cell lines successfully in a relative short time. The growth characteristics and differentiation conditions of suspension cells were measured at different phases. Results revealed that the optimal subculture time was 7-10 days, and the cells cultured for 30-120 days had the best differentiation ability (57.1%) and regeneration ability (20%). This study is promising in further using the suspension cell for genetic transformation and protoplasm isolation.
Zhang
Hongyu
H
Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China.
Yang
Fuyun
F
Gao
Mei
M
Xu
Peizhou
P
Zhang
Quanfang
Q
Xu
Jiandi
J
Wu
Xianjun
X
D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D003470:Culture Media; D012275:Oryza / Q000166:cytology*
D003470:Culture Media