[Effect of ligand concentration on the precision of determining the parameters of ligand-receptor interaction by serial dilution methods].
Earlier we suggested the method of serial dilution, which allows one to determine the parameters of ligand-receptor interaction even if the reactants are in a mixture and their concentrations are unknown. The method is especially useful if the liability of studied receptor does not allow its separation from corresponding ligand. The important prerequisite of the method's precision is that the concentration of the ligand should be sufficiently high comparing to the concentration of the receptor. In the present paper it was demonstrated that the method allows one to obtain sufficiently good precision even in the case when the concentration of the ligand is only one tenth of the receptor concentration.
Bobrovnik
S A
SA
D000915:Antibody Affinity; D000936:Antigen-Antibody Complex / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D007201:Indicator Dilution Techniques; D008024:Ligands; D008956:Models, Chemical; D011485:Protein Binding
D000936:Antigen-Antibody Complex; D008024:Ligands