Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates.
An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate the force of solution flowing through the capillary during the multi-steps immunoassay process. Immunoassay could be performed with many improvements-rapidity per sample as compared to the conventional micro-plate format (40 min vs. 5-8 h); lower cost and simpler as compared to fused silica capillary with covalent immobilization; and without problem of back pressure as compared to flow injection-bead based immunoassay. Performance of the sequential injection-capillary immunoassay was demonstrated by assay of sialoglycoconjugates level in human serum to differentiate cancer patients from healthy people.
Hartwell
Supaporn Kradtap
SK
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. kradtas@yahoo.com
Wannaprom
Napaporn
N
Kongtawelert
Prachya
P
Grudpan
Kate
K
D000327:Adsorption; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D017022:Flow Injection Analysis / Q000379:methods; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis*; D012795:Sialoglycoproteins / Q000032:analysis*; D055815:Young Adult
D006017:Glycolipids; D012795:Sialoglycoproteins; C009255:sialoglycolipids