CHO cells adhering to nitrogen-rich plasma-polymerised ethylene exhibit high production of a specific recombinant protein.
In many industrial applications, inadequate cell attachment can be a limitation, especially when serum-free media are used. Nitrogen-rich plasma-polymerised ethylene (PPE:N) exhibits high concentrations of polar groups that can help to promote the attachment of weakly adherent cell types. Tissue plasminogen activator-producing Chinese hamster ovary (CHO) cells, adapted to suspension, were grown in the presence PPE:N flakes and were found to adhere to them. The growth rate was reduced, but cell viability was enhanced and their metabolism was more efficient, with generally higher recombinant protein productivity. Finally, cell adhesion on PPE:N surfaces was found to be independent of integrins, and was probably mediated by certain non-specific interactions with the PPE:N surface.
Gigout
Anne
A
Department of Chemical Engineering, Ecole Polytechnique de Montréal, Montréal, QC, Canada.
Levasseur
Sophie
S
Girard-Lauriault
Pierre-Luc
PL
Buschmann
Michael D
MD
Wertheimer
Michael R
MR
Jolicoeur
Mario
M
D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D020099:Coated Materials, Biocompatible; D006224:Cricetinae; D003412:Cricetulus; D005030:Ethylenes / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008422:Materials Testing; D009584:Nitrogen / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013499:Surface Properties; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis
D020099:Coated Materials, Biocompatible; D005030:Ethylenes; D019012:Integrin beta1; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D005973:Glutamine; D019344:Lactic Acid; C036216:ethylene; D010959:Tissue Plasminogen Activator; D005947:Glucose; D009584:Nitrogen