title: [Comparison on HPLC fingerprint profiles of Lonicera japonica bud obtained by various dryness techniques].
abstract: OBJECTIVE
  To investigate ingredient differences of Lonicera japonica bud processed by different dryness techniques.
  
  
  METHODS
  The HPLC fingerprint was used to evaluate the quality of L. japonica by various dryness process. The analysis was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm). Acetonitril and water containing 0.4% H3PO4 were used as mobile phases with gradient elution. The flow rate was set at 1.0 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was at 238 nm.
  
  
  RESULTS
  The HPLC-fingerprint profiles of L. japonica from six different dryness techniques were determined. A total of 17 common peaks were found, but their contents from various dryness techniques were significantly different.
  
  
  CONCLUSIONS
  HPLC-fingerprint technique was one of potential Process Analytical Techniques (PAT) to explore the ingredient differences in dryness process.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Huimin
authors.initials: H
authors.lastname: Gao
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031239:Lonicera / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results