Activation of translation via reduction by thioredoxin-thioredoxin reductase in Saccharomyces cerevisiae.
Previously we reported that in vitro translation activity in extracts of Saccharomyces cerevisiae was stimulated by dithiothreitol (DTT) and further increased by the addition of thioredoxin (TRX1) [Choi, S.K. (2007) Thioredoxin-mediated regulation of protein synthesis by redox in Saccharomyces cerevisiae. Kor. J. Microbiol. Biotechnol. 35, 36-40]. To identify the pathway affecting translation, we cloned and purified thioredoxin reductase 1 (TRR1), thioredoxin reductase 2 (TRR2), glutaredoxin 1 (GRX1) and glutaredoxin reductase 1 (GLR1) as fusion proteins. Thioredoxin-mediated activation of translation was more effectively stimulated by NADPH or NADH than by DTT. Moreover, addition of TRR1 led to a further increase of translation in the presence of thioredoxin plus NADPH. These findings indicate that redox control via the thioredoxin-thioredoxin reductase system plays an important role in the regulation of translation.
Jun
Kyung Ok
KO
Department of Biological Sciences, College of Life Industry and Science, Sunchon National University, Jeonnam, Republic of Korea.
Song
Chin-Hee
CH
Kim
Young-Bum
YB
An
Jinseok
J
Oh
Jae Hyeun
JH
Choi
Sang Ki
SK
D015966:Gene Expression Regulation, Fungal; D054477:Glutaredoxins / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D054481:Thioredoxin Reductase 1 / Q000235:genetics / Q000378:metabolism*; D054482:Thioredoxin Reductase 2 / Q000235:genetics / Q000378:metabolism*; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D054477:Glutaredoxins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D009243:NAD; D013879:Thioredoxins; D009249:NADP; D054481:Thioredoxin Reductase 1; D054482:Thioredoxin Reductase 2