title: The HLA-G 14 bp insertion/deletion polymorphism is a putative susceptible factor for active human cytomegalovirus infection in children.
abstract: Human leukocyte antigen-G (HLA-G) expression is a potential factor for the pathogenesis of virus infection. A 14 bp insertion/deletion polymorphism (rs16375) in the 3'-untranslated region of the HLA-G gene is involved in the stability of HLA-G mRNA and HLA-G protein expression. Therefore, the HLA-G 14 bp polymorphism might be involved in human cytomegalovirus (hCMV) infection. To test a possible association between the HLA-G 14 bp deletion/insertion polymorphism and the active hCMV infection, in this study, a total of 54 patients with active hCMV infection and 165 age- and sex-matched, unrelated, normal Chinese Han population were genotyped for the 14 bp insertion/deletion polymorphism. Association of 14 bp polymorphism with hCMV urine DNA copies and the odds ratio (OR) of the polymorphism as a risk factor for active hCMV infection were analyzed. Our results showed that the prevalence of -14 bp/ -14 bp genotype in active hCMV patients was markedly increased [P(c) = 0.00034, OR = 3.31, 95% confidence interval (CI): 1.77-6.18], and similar significance was also observed for the frequency of -14 bp allele (P c = 0.0023, OR = 2.24, 95% CI: 1.38-3.64) when compared with that of healthy controls. Furthermore, urine hCMV DNA copies in patients with the -14 bp/ -14 bp genotype were significantly higher than those in patients with the +14 bp/ +14 bp genotype (P = 0.041). Our findings support a potential role of HLA-G 14 bp insertion/deletion polymorphism as a susceptible factor for the active hCMV infection.
authors.affiliation: Department of Laboratory Medicine, The Second Hospital, Wenzhou Medical College, Zhejiang, China.
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Zheng
authors.forename: F
authors.initials: F
authors.lastname: Zhu
authors.forename: W-W
authors.initials: WW
authors.lastname: Shi
authors.forename: A
authors.initials: A
authors.lastname: Lin
authors.forename: W-H
authors.initials: WH
authors.lastname: Yan
mesh_terms: D016022:Case-Control Studies; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000150:complications / Q000235:genetics* / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics / Q000652:urine; D004198:Disease Susceptibility; D005260:Female; D005767:Gastrointestinal Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology*; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D006505:Hepatitis / Q000235:genetics / Q000276:immunology / Q000821:virology*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007223:Infant; D008297:Male; D016254:Mutagenesis, Insertional; D011014:Pneumonia / Q000235:genetics / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics / Q000276:immunology / Q000821:virology*; D017384:Sequence Deletion
chemical_list: D004279:DNA, Viral; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G; D007075:Immunoglobulin M