A comparative study of HIV-1 clade C env evolution in a Zambian infant with an infected rhesus macaque during disease progression.
OBJECTIVE
To evaluate whether HIV-1 clade C (HIV-C) envelope variations that arise during disease progression in rhesus macaque model reflect changes that occur naturally in human infection.


METHODS
An infant macaque was infected with SHIV-1157i, an R5 tropic clade C SHIV, that expresses a primary HIV-C envelope derived from an infected human infant and monitored over a 5-year period. Genetic variation of the V1-V5 envelope region, which is the main target for humoral immune responses, derived from the infected macaque and infant was examined.


METHODS
The V1-V5 envelope region was cloned and sequenced from longitudinal peripheral blood mononuclear cell samples collected from the infected macaque and infant. Phylogenetic analysis [phylogenetic tree, diversity, divergence, ratio of nonsynonymous (dN) and synonymous substitution (dS) and dN distribution] was performed. Plasma RNA viral load, CD4(+) T-cell count, changes in the length of V1-V5 region, putative N-linked glycosylation site number and distribution were also measured.


RESULTS
Phylogenetic analysis revealed that changes in the macaque closely reflected those of the infant during disease progression. Similar distribution patterns of dN and hot spots were observed between the macaque and infant. Analysis of putative N-linked glycosylation sites revealed several common variations between the virus populations in the two host species. These variations correlate with decline of CD4 T-cell count in the macaque and might be linked with disease progression.


CONCLUSIONS
SHIV-C infection of macaque is a relevant animal model for studying variation of primary HIV-C envelope during disease progression and could be used to analyze the selection pressures that are associated with those changes.
Tso
For Yue
FY
Nebraska Center for Virology and the School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900, USA.
Hoffmann
Federico G
FG
Tully
Damien C
DC
Lemey
Philippe
P
Rasmussen
Robert A
RA
Zhang
Hong
H
Ruprecht
Ruth M
RM
Wood
Charles
C
D000595:Amino Acid Sequence; D000818:Animals; D018791:CD4 Lymphocyte Count; D018450:Disease Progression; D019143:Evolution, Molecular; D014644:Genetic Variation; D006031:Glycosylation; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000097:blood; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology*; D019562:Viral Load; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
D012367:RNA, Viral; D054299:env Gene Products, Human Immunodeficiency Virus