Functional analyses of Src-like adaptor (SLA), a glucocorticoid-regulated gene in acute lymphoblastic leukemia.
Glucocorticoids (GCs) cause apoptosis and cell cycle arrest in lymphoid cells and are used in the therapy of lymphoid malignancies. SLA (Src-like-adaptor), an inhibitor of T- and B-cell receptor signaling, is a promising candidate derived from expression profiling analyses in children with acute lymphoblastic leukemia (ALL). Over-expression and knock-down experiments in ALL in vitro model revealed that transgenic SLA alone had no effect on survival or cell cycle progression, nor did it affect sensitivity to, or kinetics of, GC-induced apoptosis. Although SLA is a prominent GC response gene, it does not seem to contribute to the anti-leukemic effects of GC.
Mansha
Muhammad
M
Division Molecular Pathophysiology, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.
Carlet
Michela
M
Ploner
Christian
C
Gruber
Georg
G
Wasim
Muhammad
M
Wiegers
Gerrit Jan
GJ
Rainer
Johannes
J
Geley
Stephan
S
Kofler
Reinhard
R
D048868:Adaptor Proteins, Signal Transducing / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002478:Cells, Cultured; D004318:Doxycycline / Q000494:pharmacology / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D019169:Jurkat Cells; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology
D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; D005938:Glucocorticoids; D034741:RNA, Small Interfering; C490635:SLA protein, human; D016392:Proto-Oncogene Proteins pp60(c-src); D004318:Doxycycline