title: [Effect of p21-activated kinase-1 on the invasiveness of colorectal carcinoma cells in vitro].
abstract: OBJECTIVE
  To observe the effect of p21-activated kinase-1 (PAK1) gene transfection on the invasiveness of human colorectal carcinoma SW480 cells in vitro.
  
  
  METHODS
  SW480 cells in routine culture were transfected with the recombinant plasmid EGFP-C1/PAK1 via Lipofectamine(TM) 2000. The expression of PAK1 protein in SW480 cells was detected using Western blotting, and the changes of the invasiveness of SW480 cells were evaluated using Boyden chamber invasion assay.
  
  
  RESULTS
  Forty-eight hours after transfection with pEGFP-C1/ PAK1, the PAK1 protein expression increased significantly in comparison with those in negative and vector control groups. The invasiveness of the SW480 cells was significantly enhanced after the transfection.
  
  
  CONCLUSIONS
  The PAK1 gene transfection can increase the expression of PAK1 in SW480 cells and enhance the invasiveness of the cells. PAK1 can be associated with the invasiveness and metastasis of colorectal carcinoma cells.
authors.affiliation: Institute of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.: jqbwu@163.com
authors.forename: Jin-Bao
authors.initials: JB
authors.lastname: Wu
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Han
authors.forename: Qing-Zhen
authors.initials: QZ
authors.lastname: Nan
authors.forename: Zhen-Shu
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Yu-Gang
authors.initials: YG
authors.lastname: Song
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010957:Plasmids; D014162:Transfection; D054462:p21-Activated Kinases / Q000235:genetics*
chemical_list: C515903:PAK1 protein, human; D054462:p21-Activated Kinases