Implication of signaling pathways involving calcium, phosphorylation and active oxygen species in methyl jasmonate-induced defense responses in grapevine cell cultures.
Perception of elicitors triggers plant defense responses via various early signal transduction pathways. Methyl jasmonate (MeJA) stimulates defense responses in grapevine (Vitis vinifera). We investigated the involvement of various partners (calcium, ROS, reversible phosphorylation) in MeJA-induced responses by using a pharmacological approach. We used specific calcium channel effectors and inhibitors of serine/threonine phosphatases, superoxide dismutase and NAD(P)H oxidase and investigated production of stilbenes (resveratrol and its glucoside, piceid, the major form), which are the grapevine phytoalexins. RNA accumulation of two genes encoding enzymes involved in stilbene synthesis (PAL and STS), three genes encoding pathogenesis-related proteins (CHIT4C, PIN and GLU) and one gene encoding an enzyme producing jasmonates (LOX) were also assessed. Calcium and its origin seemed to play a major role in MeJA-induced grapevine defense responses. Phytoalexin production was strongly affected if calcium from the influx plasma membrane was inhibited, whereas calcium from the intracellular compartments did not seem to be involved. ROS production seemed to interfere with MeJA-stimulated defense responses, and protein phosphorylation/dephosphorylation events also played a direct role.
Faurie
Bertrand
B
Groupe d'Etude des Substances Végétales à Activité Biologique, EA 3675, UFR Sciences Pharmaceutiques, Université de Bordeaux, ISVV Bordeaux-Aquitaine-CS 50008-210, Chemin de Leysotte, 33882 Villenave d'Ornon Cedex, France.
Cluzet
Stéphanie
S
Mérillon
Jean-Michel
JM
D000085:Acetates / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D002193:Cantharidin / Q000494:pharmacology; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D003998:Dicarboxylic Acids / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005960:Glucosides / Q000378:metabolism; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors; D054883:Oxylipins / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000077185:Resveratrol; D015398:Signal Transduction; D013267:Stilbenes / Q000378:metabolism; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors; D027843:Vitis / Q000187:drug effects / Q000378:metabolism*
D000085:Acetates; D003517:Cyclopentanes; D003998:Dicarboxylic Acids; D004791:Enzyme Inhibitors; D005960:Glucosides; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D017382:Reactive Oxygen Species; D013267:Stilbenes; C100030:endothall; C072239:methyl jasmonate; D013482:Superoxide Dismutase; D019255:NADPH Oxidases; D010744:Phosphoric Monoester Hydrolases; D002193:Cantharidin; D000077185:Resveratrol; D002118:Calcium; C058229:polydatin