title: An environmentally friendly reflectometric method for bumetanide determination in pharmaceuticals.
abstract: This paper describes a green analytical procedure for the determination of bumetanide using diffuse reflectance spectroscopy. The proposed method is based on reflectance measurements of a violet compound produced from a spot test reaction between bumetanide and p-dimethylaminocinnamaldehyde (p-DAC) in an acid medium, using filter paper as a solid support. The best conditions for the reaction have been found by experimental design methodologies. All reflectance measurements were carried out at 525 nm, and the linear range was from 1.37 x 10(-4) to 1.37 x 10(-3) mol L(-1), with a correlation coefficient of 0.998. The detection limit was estimated to be 3.98 x 10(-5) mol L(-1). Five commercial medicines containing bumetanide were analyzed by the proposed method. No interferences were observed from the common excipients present in pharmaceutical formulations. The results were favorably compared with those obtained by the United States Pharmacopoeia procedure at 95% confidence level.
authors.affiliation: São Paulo State University-UNESP, Institute of Chemistry of Araraquara, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Pollo
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Sequinel
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Rufino
authors.forename: Patrícia
authors.initials: P
authors.lastname: Los Weinert
authors.forename: Helena Redigolo
authors.initials: HR
authors.lastname: Pezza
authors.forename: Leonardo
authors.initials: L
authors.lastname: Pezza
mesh_terms: D002034:Bumetanide / Q000032:analysis* / Q000737:chemistry; D002934:Cinnamates / Q000737:chemistry; D003116:Color; D004232:Diuretics / Q000032:analysis* / Q000737:chemistry; D005374:Filtration; D055772:Green Chemistry Technology / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008027:Light; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D013997:Time Factors
chemical_list: D002934:Cinnamates; D004232:Diuretics; D004364:Pharmaceutical Preparations; D002034:Bumetanide; C018523:4-dimethylaminocinnamaldehyde