title: Phostensin caps to the pointed end of actin filaments and modulates actin dynamics.
abstract: Phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949, consists of 165 amino acids and is located between HLA-C and HLA-E gene clusters on human chromosome 6. In this current study, we characterized the biochemical functions of phostensin. Actin dynamics assays using gelsolin-actin seeds showed that phostensin decreases the elongation and depolymerization rates of actin filament pointed ends. The feature of phostensin that binds to the pointed ends of actin filaments was observed through fluorescent single filament binding assay. Taken together, our results suggested that phostensin is an actin filament pointed end-capping protein that is capable of modulating actin dynamics.
authors.affiliation: Department of Medicine, DaLin Tzu Chi Buddhist Hospital, Chia-Yi, Taiwan.
authors.forename: Ning-Sheng
authors.initials: NS
authors.lastname: Lai
authors.forename: Tzu-Fan
authors.initials: TF
authors.lastname: Wang
authors.forename: Shiu-Lan
authors.initials: SL
authors.lastname: Wang
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Chen
authors.forename: Jia-yi
authors.initials: JY
authors.lastname: Yen
authors.forename: Hsien-lu
authors.initials: HL
authors.lastname: Huang
authors.forename: Chin
authors.initials: C
authors.lastname: Li
authors.forename: Kuang-Yung
authors.initials: KY
authors.lastname: Huang
authors.forename: Su-Qin
authors.initials: SQ
authors.lastname: Liu
authors.forename: Ta-Hsien
authors.initials: TH
authors.lastname: Lin
authors.forename: Hsien-bin
authors.initials: HB
authors.lastname: Huang
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D006801:Humans; D054645:Protein Phosphatase 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; C520010:PPP1R18 protein, human; D054645:Protein Phosphatase 1