title: Parallel gene cloning and protein production in multiple expression systems.
abstract: To quickly find an optimal expression system for recombinant protein production, a set of vectors with the same restriction sites were constructed for parallel cloning of a target gene and recombinant protein production in prokaryotic and eukaryotic expression systems, simultaneously. These vectors include nucleotide sequences encoding protein tags and protease recognition sites for tag removal, followed by the cloning sites 5'-EcoRI/3'-XhoI identical in these vectors for ligating with the sticky-end PCR product of a target gene. Our vectors allow parallel gene cloning and protein production in multiple expression systems with minimal cloning effort.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Wang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shih
authors.forename: Su-Ming
authors.initials: SM
authors.lastname: Hu
authors.forename: Wen-Tsung
authors.initials: WT
authors.lastname: Lo
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Lin
authors.forename: Shih-Shiu
authors.initials: SS
authors.lastname: Ding
authors.forename: Hsin-Chi
authors.initials: HC
authors.lastname: Liao
authors.forename: Po-Huang
authors.initials: PH
authors.lastname: Liang
mesh_terms: D016367:Baculoviridae / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D002479:Inclusion Bodies / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins