Tissue expression of manganese superoxide dismutase is a candidate prognostic marker for glioblastoma.
BACKGROUND
Characterization of a rare subgroup of glioblastoma patients who survive for more than 3 years (long-term survival glioblastoma, LTSGBL, patients) may be helpful to identify prognostic factors.


METHODS
A molecular-profiling proteomic approach using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify prognostic factors associated with glioblastoma by comparing frozen tumor tissue from LTSGBL patients with matched samples from short-term survival glioblastoma (STSGBL) patients. Western blot (WB) analysis, reverse-transcriptase polymerase chain reaction (RT-PCR) and immmunohistochemical (IHC) staining were used for confirmation.


RESULTS
Among most candidate spots identified by 2-DE, lack of overexpression of manganese superoxide dismutase (MnSOD) in LTSGBL samples was consistently observed using WB and RT-PCR.


CONCLUSIONS
These results suggest that MnSOD expression level in tumor tissue is a candidate marker for the prognosis of glioblastoma patients.
Park
Chul-Kee
CK
Department of Neurosurgery, Seoul National University College of Medicine, Seoul, Korea.
Jung
Ji Hye
JH
Moon
Min Jeong
MJ
Kim
Young-Yim
YY
Kim
Jin Hyun
JH
Park
Sung-Hye
SH
Kim
Chae-Yong
CY
Paek
Sun Ha
SH
Kim
Dong Gyu
DG
Jung
Hee-Won
HW
Cho
Byung-Kyu
BK
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000201:enzymology* / Q000401:mortality; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005909:Glioblastoma / Q000175:diagnosis / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase / Q000032:analysis*; D000099156:Superoxide Dismutase 2
D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2