Roles of bile acid conjugates and phospholipids in in vitro activation of pancreatic lipase by bear bile and cattle bile.
BACKGROUND
Bear bile (BB) originally used as a traditional Chinese medicine has also been adopted in Japan as a traditional home remedy mainly for gastrointestinal problems due to impaired digestion. However, recently, efforts have been made to find alternatives to BB for ecological and ethical reasons.


OBJECTIVE
To find alternatives to BB for facilitating fat digestion, we compared the potency of cattle bile (CB) or synthetic mixtures of major bile components to activate pancreatic lipase with that of BB.


METHODS
The compositions of bile acid conjugates and phospholipids in BB and CB were determined by high-performance liquid chromatography and thin layer chromatography, respectively. The effects of BB and CB as well synthetic mixtures of bile acid conjugates and phospholipids on pancreatic lipase activity in vitro were examined.


RESULTS
BB and CB contained markedly different types and quantities of bile acid conjugates and phospholipids, although the potencies of BB and CB to activate pancreatic lipase were not significantly different. The potency of BB to activate pancreatic lipase was reconstituted by the major bile acid conjugates and phospholipids found in BB. In contrast, only bile acid conjugates found in CB could reconstitute its potency to activate pancreatic lipase.


CONCLUSIONS
Our observations indicate that CB or the synthetic mixture of bile components can be used as an alternative to BB for facilitating fat digestion.
Watanabe
Shiro
S
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. shirowat@inm.u-toyama.ac.jp
Kamei
Takashi
T
Tanaka
Ken
K
Kawasuji
Kunio
K
Yoshioka
Tsuyoshi
T
Ohno
Masahiro
M
D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008049:Lipase / Q000378:metabolism*; D009945:Organotherapy; D010179:Pancreas / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D001503:Ursidae
D001647:Bile Acids and Salts; D010743:Phospholipids; D008049:Lipase