Isolation and characterization of mesenchymal stem cells from the sub-amniotic human umbilical cord lining membrane.
The use of human stem cells (SCs) is a promising novel approach for the treatment of many diseases and injuries. Umbilical cord and amniotic membrane represent good sources for SCs, because they are abundant sources and there are less ethical issues unlike embryonic SCs. We aimed to isolate and characterize adult SCs from the subamnion region of the umbilical cord/amniotic membrane. Because mesenchymal stem cells (MSCs) are thought to show less immunogenicity, we first focused on the characterization of MSCs. Significant expression of typical SC-specific markers, such as SSEA-4, Oct-4, and Nanog was observed. Subamniotic MSCs did not lose the expression of Oct-4 and Nanog after freeze-thawing. Cell surface expression of MSC markers (CD73 and CD105) was confirmed by flow cytometry, and cells also differentiated into adipogenic, osteogenic, and chondrogenic lineages. On the other hand, typical embryonic SC-specific markers were not expressed and the cells also did not grow in soft agar. Thus, the subamniotic MSCs are distinct from embryonic SCs and do not show tumorigenicity in vitro. The cord lining membrane (subamniotic) MSCs isolated by our method maintain typical characteristics of MSCs in vitro, but also showed several specific features.
Kita
Katsuhiro
K
Department of Surgery and Shriners Hospitals for Children, The University of Texas Medical Branch, Galveston, Texas 77550, USA. kakita@utmb.edu
Gauglitz
Gerd G
GG
Phan
Thang T
TT
Herndon
David N
DN
Jeschke
Marc G
MG
D015720:5'-Nucleotidase / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D000650:Amnion / Q000166:cytology* / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D002471:Cell Transformation, Neoplastic; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D015925:Cryopreservation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D000071063:Endoglin; D005434:Flow Cytometry; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010011:Osteocytes / Q000166:cytology / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens / Q000235:genetics / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology* / Q000378:metabolism
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D011956:Receptors, Cell Surface; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4; D015720:5'-Nucleotidase