An N-methylpolyvinylpyridinium cationic polymer for capillary coating in electrophoresis of proteins and peptides.
A novel cationic polymeric coating is here reported, consisting of a N-methylpolyvinylpyridinium quaternary ion. This coating, combined with an isoelectric, aspartic acid as BGE, offers excellent separations of low- to high-M(r) proteins, of low- to high-pI values, coupled to very high run-to-run repeatability. The importance of a hydrophilic coating is also illustrated, whereas the N-methylpolyvinylpyridinium quaternary ion does not seem to adsorb even a large size protein as human albumin, separations are already distorted in the N-ethyl derivative and totally ruined in the N-octyl derivative, due to its high hydrophobicity.
Sebastiano
Roberto
R
Politecnico di Milano, Department of Chemistry, Via Mancinelli 7, Milano 20131, Italy.
Mendieta
Martha E
ME
Contiello
Nunzia
N
Citterio
Attilio
A
Righetti
Pier Giorgio
PG
D000085:Acetates / Q000737:chemistry; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000379:methods*; D005561:Formates / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis; D011145:Polyvinyls / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D011726:Pyridinium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D013499:Surface Properties
D000085:Acetates; D002412:Cations; D005561:Formates; C550523:N-methylpolyvinylpyridinium; D010455:Peptides; D011145:Polyvinyls; D011506:Proteins; D011726:Pyridinium Compounds; C030544:formic acid; D001224:Aspartic Acid; C018824:ammonium acetate