title: Transport of live yeast and zebrafish embryo on a droplet digital microfluidic platform.
abstract: We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays.
authors.affiliation: Department of Chemistry and Biochemistry and California NanoSystems Institute, University of California Los Angeles, CA 90095-1569, USA.
authors.forename: Sang Uk
authors.initials: SU
authors.lastname: Son
authors.forename: Robin L
authors.initials: RL
authors.lastname: Garrell
mesh_terms: D000818:Animals; D046149:Embryo Culture Techniques; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009038:Motion; D011309:Preservation, Biological / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D015027:Zebrafish / Q000196:embryology*