Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1.
Plasma membrane of the yeast Saccharomyces cerevisiae contains stable lateral domains. We have investigated the ultrastructure of one type of domain, the membrane compartment of Can1 (MCC). In two yeast strains (nce102Delta and pil1Delta) that are defective in segregation of MCC-specific proteins, we found the plasma membrane to be devoid of the characteristic furrow-like invaginations. These are highly conserved plasma membrane structures reported in early freeze-fracture studies. Comparison of the results obtained by three different approaches - electron microscopy of freeze-etched cells, confocal microscopy of intact cells and computer simulation - shows that the number of invaginations corresponds to the number of MCC patches in the membrane of wild-type cells. In addition, neither MCC patches nor the furrow-like invaginations colocalized with the cortical ER. In mutants exhibiting elongated MCC patches, there are elongated invaginations of the appropriate size and frequency. Using various approaches of immunoelectron microscopy, the MCC protein Sur7, as well as the eisosome marker Pil1, have been detected at these invaginations. Thus, we identify the MCC patch, which is a lateral membrane domain of specific composition and function, with a specific structure in the yeast plasma membrane - the furrow-like invagination.
Strádalová
Vendula
V
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Stahlschmidt
Wiebke
W
Grossmann
Guido
G
Blazíková
Michaela
M
Rachel
Reinhard
R
Tanner
Widmar
W
Malinsky
Jan
J
D026923:Amino Acid Transport Systems, Basic / Q000378:metabolism*; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D022081:Cell Surface Extensions / Q000378:metabolism* / Q000648:ultrastructure; D003198:Computer Simulation; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism* / Q000648:ultrastructure; D013499:Surface Properties; D016610:Tissue Embedding
D026923:Amino Acid Transport Systems, Basic; C502212:CAN1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins