title: [Regulation of c-fos gene expression in hepatic stellate cells by transforming growth factor beta].
abstract: OBJECTIVE
  To investigate the effects of transforming growth factor beta (TGF ) on c-fos gene expression in hepatic stellate cells.
  
  
  METHODS
  Hepatic stellate cells (HSC-T6) were cultured in the medium containing different concentrations of TGF (0.2, 1, and 5 ng/ml), and cells were collected at different time points of incubation (8, 24, 48, and 72 h). The total RNA of the HSCs was isolated and c-fos gene expression level were measured by reverse transcription polymerase chain reaction.
  
  
  RESULTS
  c-fos gene expression levels of HSCs cultured in the presence of low (0.2 ng/ml), moderate (1 ng/ml) and high (5 ng/ml) concentrations of TGF for 8, 24, 48 and 72 h were significantly greater than those of control group. The c-fos gene expression levels of HSCs increased gradually with the increment of TGF concentration, and significant differences in c-fos gene expression were found between the 3TGF groups.
  
  
  CONCLUSIONS
  TGF strongly up-regulates c-fos gene expression in hepatic stellate cells.
authors.affiliation: Department of Gastroenterology, 252 Hospital of PLA, Baoding 071000, China. aiminwang62@sina.com
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Wang
authors.forename: Chun-Gai
authors.initials: CG
authors.lastname: Ren
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiang
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Zhu
authors.forename: Bao-En
authors.initials: BE
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos; D055166:Hepatic Stellate Cells / Q000187:drug effects*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D016212:Transforming Growth Factor beta