[Microsatellite alternation in laryngeal squamous cell carcinomas].
OBJECTIVE
To examine the microsatellite instability and loss of heterozygosity in the pathogenic mechanism of laryngeal squamous cell carcinomas.


METHODS
Forty cases squamous cell carcinomas of larynx were analyzed by comparing tumorous tissues and normal tissues around with 3 microsatellite markers from chromosome 3, 5 and 11, using PCR and PGE-AgNO3 staining.


RESULTS
Among the 40 cases of laryngeal squamous cell carcinomas, 87.5% (35/40) of samples showed microsatellite instability or loss of heterozygosity in one to three microsatellite markers. High frequent microsatellite abnormal occurred at D5S592, it was 70% (28/40). Then the mutation rate of D3s1228 was 52.5% (21/40).


CONCLUSIONS
Our study revealed that tumor suppressor genes nearby chromosome 3p14 and 5q23 regions related to the pathogenesis of squamous cell carcinomas of larynx. A correlation between microsatellite alternation and stage of the tumor were found in D3s1228 and D5s592 chromosome regions.
Chen
Feifei
F
Department of Otorhinolaryngology-Head and Neck Surgery, the First Hospital of Medical College, Jilin University, Changchun, 130021, China.
Zhu
Wei
W
Liu
Bing
B
Yu
Hong
H
Ruan
Yang
Y
Zhang
Yuanding
Y
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016147:Genes, Tumor Suppressor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D019656:Loss of Heterozygosity; D053842:Microsatellite Instability; D009367:Neoplasm Staging