Soy isoflavones mitigate long-term femoral and lumbar vertebral bone loss in middle-aged ovariectomized mice.
We evaluated the protective effects of soy isoflavones (SIF) against osteoporosis in middle-aged ovariectomized (OVX) mice. SIF (30 mg/kg or 60 mg/kg) or 17beta-estradiol (E(2)) was administered to OVX mice for 4 months after bilateral ovariectomy. We observed the biochemical markers of bone turnover, e.g., alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), in serum. We also observed the bone mineral density (BMD) in femurs and lumbar vertebrae. In addition, we examined trabecular bone and interstitial cells in the femur using hematoxylin and eosin staining. The decrease in ALP levels and the increase in TRAP levels normally resulting from ovariectomy were suppressed by administration of 60 mg/kg SIF or E(2). Administration of 60 mg/kg SIF or E(2) also maintained the BMD, trabecular bone, and interstitial cells in OVX mice compared to those in pre-OVX mice. These results suggest that 60 mg/kg SIF effectively mitigates ovariectomy-induced osteoporosis in middle-aged mice.
Kim
Dae Won
DW
Research Center, Natural F&P Corp., Hallym University, Chuncheon, Republic of Korea.
Yoo
Ki-Yeon
KY
Lee
Yoon-Bok
YB
Lee
Keun-Ha
KH
Sohn
Heon-Soo
HS
Lee
Sung-Joon
SJ
Cho
Kyung-Hwan
KH
Shin
Yong Kook
YK
Hwang
In Koo
IK
Won
Moo-Ho
MH
Kim
Dong-Woo
DW
D000135:Acid Phosphatase / Q000097:blood; D000375:Aging / Q000187:drug effects / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D050071:Bone Density Conservation Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004958:Estradiol / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D005269:Femur / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D008159:Lumbar Vertebrae / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D010024:Osteoporosis / Q000097:blood / Q000517:prevention & control*; D010052:Ovariectomy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D013025:Glycine max / Q000737:chemistry*
D050071:Bone Density Conservation Agents; D007529:Isoflavones; D010936:Plant Extracts; D004958:Estradiol; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase