1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA.
The backbone and side chain resonance assignments of an engineered intein based on Mycobacterium tuberculosis RecA have been determined based on triple-resonance experiments with the uniformly [(13)C,(15)N]-labeled protein.
Du
Zhenming
Z
Biology Department, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Liu
Yangzhong
Y
Zheng
Yuchuan
Y
McCallum
Scott
S
Dansereau
John
J
Derbyshire
Victoria
V
Belfort
Marlene
M
Belfort
Georges
G
Van Roey
Patrick
P
Wang
Chunyu
C
D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D047668:Inteins; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D011522:Protons; D011938:Rec A Recombinases / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D011938:Rec A Recombinases