title: Cardiomyoblast-like cells differentiated from human adipose tissue-derived mesenchymal stem cells improve left ventricular dysfunction and survival in a rat myocardial infarction model.
abstract: Adipose tissue-derived mesenchymal stem cells (ADMSCs) are multipotent cells. Here we examined whether human ADMSCs (hADMSCs) could differentiate into cardiomyoblast-like cells (CLCs) by induction with dimethylsulfoxide and whether the cells would be utilized to treat cardiac dysfunction. Dimethylsulfoxide induced the expression of various cardiac markers in hADMSCs, such as alpha-cardiac actin, cardiac myosin light chain, and myosin heavy chain; none of which were detected in noncommitted hADMSCs. The induced cells were thus designated as hADMSC-derived CLCs (hCLCs). To confirm their beneficial effect on cardiac function, hCLC patches were transplanted onto the Nude rat myocardial infarction model, and compared with noncommitted hADMSC patch transplants and sham operations. Echocardiography demonstrated significant short-term improvement of cardiac function in both the patch-transplanted groups. However, long-term follow-up showed rescue and maintenance of cardiac function in the hCLC patch-transplanted group only, but not in the noncommitted hADMSC patch-transplanted animals. The hCLCs, but not the hADMSCs, engrafted into the scarred myocardium and differentiated into human cardiac troponin I-positive cells, and thus regarded as cardiomyocytes. Transplantation of the hCLC patches also resulted in recovery of cardiac function and improvement of long-term survival rate. Thus, transplantation of hCLC patches is a potentially effective therapeutic strategy for future cardiac tissue regeneration.
authors.affiliation: Department of Somatic Stem Cell Therapy, Foundation for Biomedical Research and Innovation , Kobe, Japan .
authors.forename: Hanayuki
authors.initials: H
authors.lastname: Okura
authors.forename: Akifumi
authors.initials: A
authors.lastname: Matsuyama
authors.forename: Chun-Man
authors.initials: CM
authors.lastname: Lee
authors.forename: Ayami
authors.initials: A
authors.lastname: Saga
authors.forename: Aya
authors.initials: A
authors.lastname: Kakuta-Yamamoto
authors.forename: Anna
authors.initials: A
authors.lastname: Nagao
authors.forename: Nagako
authors.initials: N
authors.lastname: Sougawa
authors.forename: Naosumi
authors.initials: N
authors.lastname: Sekiya
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takekita
authors.forename: Yashuhiro
authors.initials: Y
authors.lastname: Shudo
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyagawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Komoda
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D001483:Base Sequence; D017690:Cell Transplantation; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018487:Ventricular Dysfunction, Left / Q000503:physiopathology / Q000628:therapy*
chemical_list: D017931:DNA Primers