title: 1H, 15N and 13C assignments of yellow fluorescent protein (YFP) Venus.
abstract: We present here the backbone and side-chain NMR assignments of YFP Venus, a 238-residue protein that emits yellow fluorescence in its native state. Venus is a variant of the green fluorescent protein (GFP), which has improved chromophore maturation and brightness, and the photochemistry and photophysics of which are insensitive to experimental conditions, such as the pH value and buffer content, making it a favourable biomarker.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. stdh2@cam.ac.uk
authors.forename: Shang-Te Danny
authors.initials: ST
authors.lastname: Hsu
authors.forename: Caroline
authors.initials: C
authors.lastname: Behrens
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Cabrita
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D008164:Luminescent Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000737:chemistry; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D008164:Luminescent Proteins; D009587:Nitrogen Isotopes; D011522:Protons