title: Selective cytotoxic effects on human cancer cell lines of phenolic-rich ethyl-acetate fraction from Rhus verniciflua Stokes.
abstract: Rhus verniciflua Stokes (RVS) is a plant with a long history of medicinal use in Eastern Asia. RVS has been widely used to treat gastritis, stomach cancer and atherosclerosis. The cytotoxic effects of different solvent fractions from an RVS ethanol extract were measured in 11 human cancer cell lines. The study showed that the ethyl-acetate (EtOAC) fraction was the most cytotoxic. This fraction contains a number of phenolic compounds, and this phenolic-rich EtOAC fraction was particularly effective against gastric and breast cancer cells. A purified phenolic-rich EtOAC fraction (PPEF) had a stronger apoptotic effect on these cells. Liquid chromatography-mass spectrometry (LC-MS) analysis showed that the PPEF contained gallic acid, protocatechuic acid, fisetin, sulfuretin, butein and 8 unknown compounds. There were only small amounts of flavonoids: fisetin, sulfuretin and butein. The results showed that PPEF induces apoptosis only in gastric and breast cancer cell lines, but not in lung, colon or liver cancer cell lines. Therefore, PPEF may have a significant potential as an organ-specific anti-cancer agent.
authors.affiliation: Department of Basic Science of Oriental Medicine, Kyunghee University, Seoul 130-701, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kim
authors.forename: Chang Hwa
authors.initials: CH
authors.lastname: Jung
authors.forename: Bo-Hyoung
authors.initials: BH
authors.lastname: Jang
authors.forename: Ho Yeon
authors.initials: HY
authors.lastname: Go
authors.forename: Jong-Hyeong
authors.initials: JH
authors.lastname: Park
authors.forename: You-Kyung
authors.initials: YK
authors.lastname: Choi
authors.forename: Seong Il
authors.initials: SI
authors.lastname: Hong
authors.forename: Yong Cheol
authors.initials: YC
authors.lastname: Shin
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Ko
mesh_terms: D000085:Acetates; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D000431:Ethanol; D005260:Female; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms; D008175:Lung Neoplasms; D019800:Phenol; D029045:Rhus / Q000737:chemistry*; D012997:Solvents; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000085:Acetates; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012997:Solvents; D019800:Phenol; D000431:Ethanol; C007650:ethyl acetate