[Effects of the treatment with different fluids on alveolar epithelium barrier in rats with acute lung injury].
OBJECTIVE
To observe the effects of different fluids on alveolar epithelium barrier in rats with acute lung injury (ALI).


METHODS
Thirty-six Sprague-Dawley (SD) rats were randomly assigned into six groups with 6 rats in each group. ALI was induced by intravenous injection of lipopolysaccharide(LPS). Rats in all treatment groups were given different fluids and sacrificed after 4 hours. Evans blue dye (EBD) was injected via the femoral vein 30 minutes before death. Tracheobronchial tree was washed with normal saline (NS) after death, and broncho-alveolar lavage fluid (BALF) was collected. Leakage of EBD from blood into BALF (alveolar epithelial permeability) and wet/dry (W/D) ratio were measured. The mRNA expression of surfactant protein-C (SP-C) was assessed by reverse transcription-polymerase chain reaction (RT-PCR). Alveolar epithelium apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL). Lung injury was evaluated by Smith lung injury score.


RESULTS
(1) Lung injury scores in LPS and NS groups were significantly higher than in control group (both P < 0.05). Compared with NS group, lung injury scores were significantly lower in 5% albumin (ALB) and 6% hydroxyethyl starch 130/0.4 (HES) groups (both P < 0.05), and there was no significant difference in 4% succinylated gelatin (GEL) group (P > 0.05). No significant difference was found among the latter three groups. (2) W/D ratio in LPS and NS groups were significantly higher than that in control group (both P < 0.05). Compared with NS group, W/D ratios were lower in ALB, HES and GEL groups (all P < 0.05). But it showed no significant difference among the latter three groups. (3) Alveolar epithelial permeability in LPS and NS groups were remarkably higher than that in control group (both P < 0.05). Compared with NS group, the alveolar epithelial permeability were significantly lower in ALB, HES and GEL groups (all P < 0.05). The alveolar epithelial permeability in the latter two groups were significant lower than that in ALB group (both P < 0.05) but higher than that of control group. (4) The SP-C mRNA expression in LPS, NS, ALB and GEL groups were lower than that in control and HES groups (all P < 0.05), but there was no difference between control and HES groups (P > 0.05). (5) Apoptosis index (AI) of alveolar epithelial cell in all the treatment groups were significantly higher than that in control group (all P < 0.05). Compared with NS group, AI were noticeably lower in ALB and HES groups (both P < 0.05).


CONCLUSIONS
Compared with NS, colloid can probably improve the alveolar epithelial permeability and protect the barrier function.
Wei
Hong-xia
HX
Department of Infection, Nanjing Second Hospital, Southeast University, Nanjing 210003, Jiangsu, China.
Yang
Yi
Y
Qiu
Hai-bo
HB
Guo
Tao
T
Zhao
Ming-ming
MM
Chen
Qiu-hua
QH
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy; D056809:Alveolar Epithelial Cells / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D002199:Capillary Permeability; D004195:Disease Models, Animal; D005260:Female; D005440:Fluid Therapy / Q000379:methods*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides