title: In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti-6Al-4V surfaces.
abstract: Layer-by-layer self-assembled films of molecular oligoelectrolytes were used to modify Ti-6Al-4V surfaces in order to test their ability as potential drug delivery system. With regard to medical application the in vitro behavior of the modified material was investigated. The Ti-6Al-4V (6% aluminium, 4% vanadium) material was treated in a layer-by-layer (LbL) process with 2, 4, 6 and 8 layers of molecular oligoelectrolytes 1 and 2 and thereby doped with a fluorescent reporter molecule 2. Human osteoblasts were cultured for a period up to 5 days on the modified material. Ti-6Al-4V surfaces without modification were used as control. In order to investigate the in vitro behavior of the coating as well as the influence of components of the coating on osteoblastic cells, respectively, cell proliferation, differentiation and attachment of hFOB cells were observed by means of cell number, osteoblastic gene expression and fluorescence microscopy. Degradation behavior of the OEM (oligoelectrolyte multilayer film) was examined using optical spectroscopy. Measurement data imply that the layer-by-layer coating was successfully assembled on the Ti surface and endures steam sterilization. The fluorescence signal in cell culture medium increased strictly linear with increasing pre-assembled number of layers on the surface. Proliferation rates of the cells in experimental groups did not differ significantly from each other (P >or= 0.783). Differentiation pattern was not significantly changed by the coating. The fluorescent reporter component of the film was absorbed by osteoblastic cells and was detected by fluorescence microscopy.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Erlangen, Germany. sabine.ponader@uk-erlangen.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Ponader
authors.forename: Karin
authors.initials: K
authors.lastname: Rosenlehner
authors.forename: Eleftherios
authors.initials: E
authors.lastname: Vairaktaris
authors.forename: Cornelius
authors.initials: C
authors.lastname: von Wilmowsky
authors.forename: Karl A
authors.initials: KA
authors.lastname: Schlegel
authors.forename: Friedrich W
authors.initials: FW
authors.lastname: Neukam
authors.forename: Cordula D
authors.initials: CD
authors.lastname: Schmidt
authors.forename: Torsten
authors.initials: T
authors.lastname: Schunk
authors.forename: Andreas
authors.initials: A
authors.lastname: Hirsch
authors.forename: Emeka
authors.initials: E
authors.lastname: Nkenke
mesh_terms: D000497:Alloys; D001483:Base Sequence; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017931:DNA Primers; D004573:Electrolytes / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology; D016133:Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D017931:DNA Primers; D004573:Electrolytes; C031462:titanium alloy (TiAl6V4); D014025:Titanium