[Some specific effects of the combined exposure to gamma and laser radiations on survivability of mouse fibroblasts in vitro].
Effects of the combined exposure to gamma-rays and laser radiation (633 nm) on survivability of fibroblast cells C3H/10T1/2, clone 8 were studied. First, a monolayer of fibroblast cells on a plastic vial wall was irradiated by gamma-rays and then the whole wall surface (25 cm2) or the central part of the vial (1 CM2) only was exposed to laser radiation. Forty minutes after exposure the cells were dispersed in 0.25% solution of tripsin and inoculated in vials for survivability determination. The experiments evidenced that the radioprotective effect of laser is essentially invariant no matter the size of a laser-irradiated monolayer. Adding the nutrient medium from the vials subjected to the combined irradiation to the gamma-irradiated vials also increased cell survivability. Cells from the cultivated blend of suspensions (exposed to ionizing and optic radiations and to ionizing radiation only) also exhibited a better survivability. A bystander radioprotective effect of laser can be assumed.
Voskanian
K Sh
KSh
Mitsyn
G V
GV
Gaevskiĭ
V N
VN
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000473:pathology* / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects*; D053685:Laser Therapy / Q000379:methods*; D007834:Lasers; D051379:Mice; D008809:Mice, Inbred C3H; D011833:Radiation Injuries, Experimental / Q000473:pathology* / Q000517:prevention & control