A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format.
A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.
Johnsen
Anders R
AR
Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark. arj@geus.dk
Hybholt
Trine K
TK
Jacobsen
Ole S
OS
Aamand
Jens
J
D002245:Carbon Dioxide / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000378:metabolism*; D004783:Environmental Microbiology; D004784:Environmental Monitoring; D013194:Staining and Labeling / Q000379:methods*
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide