Chemical approach for specific enrichment and mass analysis of nitrated peptides.
The analysis and detection of 3-nitrotyrosine are biologically and clinically important because protein tyrosine nitration is known to be involved in a number of biological phenomena such as cellular signal transduction, pathogenesis of inflammatory responses, and age-related disorders. However, the main obstacles in the study are low abundance of nitrated species and lack of efficient enrichment methods. Here in, we suggest a new chemical approach to analyze nitrated peptides using mass spectrometry by incorporating specific tagging groups in the peptides through simple chemical transformations. Nitro groups on tyrosine side chains of nitrated peptides were subjected to reduction to give rise to amine which was further converted to metal-chelating motif. Mass analyses verified that Ni(2+)-NTA magnetic agarose beads selectively captured and isolated the modified peptides, i.e., nitrated peptides, by strong and specific metal chelating interactions. We further demonstrated the utility of our approach by detection of nitrated peptides in complex samples such as tryptic peptide mixtures of bovine serum albumin (BSA) and a HeLa cell lysate.
Lee
Jung Rok
JR
Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-834, Korea.
Lee
Soo Jae
SJ
Kim
Tae Woo
TW
Kim
Jae Kyung
JK
Park
Hyung Soon
HS
Kim
Dong-Eun
DE
Kim
Kwang Pyo
KP
Yeo
Woon-Seok
WS
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009566:Nitrates / Q000737:chemistry*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D009566:Nitrates; D010455:Peptides