Evidence of okadaic acid production in a cultured strain of the marine dinoflagellate Prorocentrum rhathymum from Malaysia.
Protein phosphatase inhibition assay (PPIA), Neuroblastoma cell-based assay (Neuro-2a CBA) and LC-MS/MS analysis revealed for the first time the production of okadaic acid (OA) by a Prorocentrum rhathymum strain. Low amounts of OA were detected by LC-MS/MS analysis. Inhibition of PP2A activity and a weak toxicity to the Neuro-2a CBA were also observed.
Caillaud
A
A
IRTA, Ctra. Poble Nou, km. 5.5, 43540 Sant Carles de la Ràpita, Spain.
de la Iglesia
P
P
Campàs
M
M
Elandaloussi
L
L
Fernández
M
M
Mohammad-Noor
N
N
Andree
K
K
Diogène
J
J
D000818:Animals; D001681:Biological Assay; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004141:Dinoflagellida / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008296:Malaysia; D013058:Mass Spectrometry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D019319:Okadaic Acid / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019319:Okadaic Acid; D004247:DNA; D010749:Phosphoprotein Phosphatases