title: A glycoprotein extracted from golden oyster mushroom Pleurotus citrinopileatus exhibiting growth inhibitory effect against U937 leukemia cells.
abstract: Mushrooms have become popular sources of natural antitumor, antiviral, antibacterial, antioxidative, and immunomodulatory agents. Golden oyster mushroom, Pleurotus citrinopileatus , is a common mushroom in oriental countries for human consumption. We isolated a functional protein (PCP-3A) from the fresh fruiting body of this mushroom. The isolation procedure included ammonium sulfate fractionation, DEAE-Sepharose CL-6B ion exchange chromatography, and Sephacryl S-300 gel filtration. Electrophoresis demonstrated that PCP-3A is a glycoprotein composed of 10 subunits, each approximately 45.0 kDa in size. In vitro cell study showed that PCP-3A at a concentration about 12.5 microg/mL inhibits the proliferation of human tumor cell line U937, in a time- dependent manner (24, 48, and 72 h). It failed to agglutinate rabbit and human erythrocytes, excluding its possibility from being a lectin. Flow cytometry revealed that it is capable of inhibiting the growth of U937 cells by way of S phase arrest and apoptotic induction. We suggest that PCP-3A is worth further investigating for antitumor use.
authors.affiliation: Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Chen
authors.forename: Yuh-Tai
authors.initials: YT
authors.lastname: Wang
authors.forename: James Swi-Bea
authors.initials: JS
authors.lastname: Wu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000276:immunology / Q000503:physiopathology*; D020076:Pleurotus / Q000737:chemistry*; D011817:Rabbits
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins