Reproducibility of 3.0 Tesla magnetic resonance spectroscopy for measuring hepatic fat content.
OBJECTIVE
To investigate reproducibility of proton magnetic resonance spectroscopy ((1)H-MRS) to measure hepatic triglyceride content (HTGC).


METHODS
In 24 subjects, HTGC was evaluated using (1)H-MRS at 3.0 Tesla. We studied "between-weeks" reproducibility and reproducibility of (1)H-MRS in subjects with fatty liver. We also studied within liver variability and within day reproducibility. Reproducibility was assessed by coefficient of variation (CV), repeatability coefficient (RC), and intraclass correlation coefficient (ICC).


RESULTS
The CV of between weeks reproducibility was 9.5%, with a RC of 1.3% HTGC (ICC 0.998). The CV in fatty livers was 4.1%, with a RC of 1.3% HTGC (ICC 0.997). Within day CV was 4.5%, with a RC of 0.4% HTGC (ICC 0.999). CV for within liver variability was 14.5%.


CONCLUSIONS
Reproducibility of (1)H-MRS to measure HTGC for "between-weeks" measurements and in fatty livers is high, which is important for follow-up studies. Within liver variability displays a larger variation, meaning that liver fat is not equally distributed and during consecutive measurements the same voxel position should be used.
van Werven
Jochem R
JR
Department of Radiology, Academic Medical Center at the University of Amsterdam, Amsterdam, the Netherlands. j.r.vanwerven@amc.uva.nl
Hoogduin
Johannes M
JM
Nederveen
Aart J
AJ
van Vliet
Andre A
AA
Wajs
Ewa
E
Vandenberk
Petra
P
Stroes
Erik S G
ES
Stoker
Jaap
J
D000328:Adult; D000368:Aged; D005234:Fatty Liver / Q000473:pathology*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications; D010865:Pilot Projects; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric