Optimization of preservatives in a topical formulation using experimental design.
Optimizing the preservative regime for a preparation requires the antimicrobial effectiveness of several preservative combinations to be determined. In this study, three preservatives were tested: benzoic acid, sorbic acid and benzylic alcohol. Their preservative effects were evaluated using the antimicrobial preservative efficacy test (challenge-test) of the European Pharmacopeia (EP). A D-optimal mixture design was used to provide a maximum of information from a limited number of experiments. The results of this study were analysed with the help of the Design Expert software and enabled us to formulate emulsions satisfying both requirements A and B of the EP.
Rahali
Y
Y
Laboratoire de Pharmacie Galénique et Pharmacotechnie Industrielle, Faculté de Médecine et de Pharmacie de Rabat, 6203, Université Mohammed V - Souissi Maroc. younesrahali@gmail.com
Pensé-Lhéritier
A-M
AM
Mielcarek
C
C
Bensouda
Y
Y
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D019817:Benzoic Acid / Q000737:chemistry* / Q000494:pharmacology; D019905:Benzyl Alcohol / Q000737:chemistry* / Q000494:pharmacology; D004655:Emulsions / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D011310:Preservatives, Pharmaceutical / Q000737:chemistry* / Q000494:pharmacology; D013011:Sorbic Acid / Q000737:chemistry* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004655:Emulsions; D011310:Preservatives, Pharmaceutical; D019817:Benzoic Acid; D019905:Benzyl Alcohol; D013011:Sorbic Acid