title: Pyrrolidine dithiocarbamate attenuates paraquat-induced lung injury in rats.
abstract: Paraquat (PQ) has been demonstrated that the main target organ for the toxicity is the lung. This study aimed to investigate the potential protective effect of PDTC on the PQ-induced pulmonary damage. Fifty-four rats were divided into control, PQ-treated and PQ+PDTC-treated groups. Rats in the PQ group were administrated 40 mg/kg PQ by gastric gavage, and PDTC group with 40 mg/kg PQ followed by injection of 120 mg/kg PDTC (IP). On the days 3, 7, 14 and 21 after treatments, the activities of GSH-Px, SOD, MDA level and the content of HYP were measured. TGF-beta1 mRNA and protein were assayed by RT-PCR and ELISA. MDA level in plasma and BALF was increased and the activities of GSH-Px and SOD were decreased significantly in the PQ-treated groups (P < .05) compared with control group. While the activities of GSH-Px and SOD in the PQ+PDTC-treated groups was markedly higher than that of PQ-treated groups (P < .05), and in contrast, MDA level was lower. TGF-beta1 mRNA and protein were significantly lower in the PQ+PDTC-treated groups than that of PQ-treated groups (P < .05). The histopathological changes in the PQ+PDTC-treated groups were milder than those of PQ groups. Our results suggested that PDTC treatment significantly attenuated paraquat-induced pulmonary damage.
authors.affiliation: Department of Occupational Health and Toxicology, School of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Chang
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Shao
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.forename: Qiangen
authors.initials: Q
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D016207:Cytokines / Q000032:analysis*; D004195:Disease Models, Animal; D006801:Humans; D055370:Lung Injury / Q000139:chemically induced* / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D010269:Paraquat; D011759:Pyrrolidines / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000032:analysis*; D013859:Thiocarbamates / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D016207:Cytokines; D011759:Pyrrolidines; D017382:Reactive Oxygen Species; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D010269:Paraquat