[Protection of radiosensitive human cells against the action of heavy metals by antimutagens and adapting factors: association with genetic and protein polymorphisms].
Cells of a diploid line obtained from embryos with the Down's syndrome, known to be unable to repair gamma-induced DNA damage, were treated with natural (garlic extract, retinol) and synthetic (crown compound) antimutagens and with adapting factors (heat shock, low CdCl2 concentrations, 10(-8) M). The protective effect was evaluated by registering DNA breaks and cell survival, and the protection coefficients were calculated. The most effective results were obtained with the use of the garlic extract and retinol. No protection of the DNA structure was observed when cells were treated with low concentrations of cadmium chloride and then with high concentrations, i. e., no adaptive response (AR) was formed under these conditions. The spectrum of proteins in treated and control cells as well as detoxication genes (GSTM1, GSTT1 , CYPIA1) were determined.
Vasil'eva
I M
IM
Shagirova
Zh M
ZhM
Sinel'shchikova
T A
TA
Mavletova
D A
DA
Kuz'mina
N S
NS
Zasukhina
G D
GD
D000222:Adaptation, Physiological; D016587:Antimutagenic Agents / Q000494:pharmacology*; D019256:Cadmium Chloride / Q000494:pharmacology / Q000633:toxicity*; D002460:Cell Line; D047248:Crown Compounds / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D004260:DNA Repair; D004314:Down Syndrome / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D005737:Garlic / Q000737:chemistry; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology; D011110:Polymorphism, Genetic; D014801:Vitamin A / Q000494:pharmacology
D016587:Antimutagenic Agents; D019256:Cadmium Chloride; D047248:Crown Compounds; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; D009153:Mutagens; D010936:Plant Extracts; D014801:Vitamin A; C413545:glutathione S-transferase T1