title: Activation of extracellular signal-regulated kinase in MDBK cells infected with bovine viral diarrhea virus.
abstract: Our efforts to identify the cellular signaling cascades triggered by bovine viral diarrhea virus (BVDV) infection in MDBK cells revealed marked activation of extracellular signal-regulated kinase 1/2 (ERK). Enhanced phosphorylation of ERK was detected following infection with cytopathogenic (cp) BVDV, but not with noncytopathogenic BVDV. It appears that cp BVDV-induced ERK phosphorylation is caused by oxidative stress, because ERK phosphorylation was inducible by treatment with hydrogen peroxide or serum deprivation and was attenuated by addition of antioxidants. These results indicate that BVDV infection influences the ERK signaling pathway via oxidative stress, depending on the biotype.
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamane
authors.forename: Muhammad Atif
authors.initials: MA
authors.lastname: Zahoor
authors.forename: Yassir Mahgoub
authors.initials: YM
authors.lastname: Mohamed
authors.forename: Walid
authors.initials: W
authors.lastname: Azab
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Tohya
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000201:enzymology* / Q000821:virology*; D002417:Cattle; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D004789:Enzyme Activation; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D018384:Oxidative Stress; D010766:Phosphorylation; D015854:Up-Regulation
chemical_list: D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3