[Characterization and biocompatibility of human-like collagen-hyaluronic acid scaffold for blood vessel].
Human-like collagen (HLC) was cross-linked with hyaluronic acid by genipin in different ratio. The concentrations of hyaluronic acid in the mixture were 0, 0.01%, 0.05% and 0.1%. The blood vessel tubular grafts were then fabricated by freeze-drying. Microstructure, element composite, mechanical properties, cytotoxicity grade, and biocompatibility of different vascular scaffold groups were studied by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), tensile test, burst pressure experiment, cytotoxicity experiment, endothelial cells planted in blood vessel scaffolds and hypodermic embedding of mice. The results showed that HLC-HA (0.05%) tubular scaffold exhibited interconnected well-distributed and porous structure and porosity of 94.38%; achieved the desirable mechanical property with stress of (1000.8 +/- 7.9) kPa and burst pressure of (1058.6 +/- 8.2) kPa, hypocytotoxicity, favourable cytocompatibility, hisocompatibility and disposition of degradation.
Sun
Xiujuan
X
Shaanxi Key Laboratory of Degradable Biomaterials, Northwest University, Xi'an 710069, China.
Fan
Daidi
D
Zhu
Chenhui
C
Ma
Xiaoxuan
X
Luo
Yane
Y
Chen
Lan
L
Guo
Jiaqing
J
D000269:Adhesives / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D057889:Iridoid Glycosides; D039823:Iridoids / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds
D000269:Adhesives; D001672:Biocompatible Materials; D057889:Iridoid Glycosides; D039823:Iridoids; D006820:Hyaluronic Acid; D003094:Collagen; C007834:genipin