[MMP-9 expression profile in inflammatory cells of Mip-1alpha knockout mice and Mip-1alpha receptor knockout mice].
OBJECTIVE
To explore the MMP-9 expression profile in peritoneal inflammatory macrophages and granulocytes in Mip-1alpha-, CCR1- and CCR5-deficient mice.


METHODS
In sodium thioglycolate-induced murine peritonitis models, peritoneal macrophages and granulocytes were harvested, identified and purified from WT mice and Mip-1alpha-, CCR1-, CCR5-deficient mice. The RT-PCR was applied to evaluate the expression of MMP-9 in macrophages and granulocytes of different group of mice.


RESULTS
The expressions of MMP-9 of macrophages in Mip-1alpha-, CCR1-, CCR5-deficient mice were significantly lower than that of WT mice (P<0.05); MMP-9 expression of granulocytes in Mip-1alpha-, CCR5-deficient mice were also significantly lower than that of WT mice (P<0.05), while the MMP-9 expression of granulocytes in CCR1-deficient mice was significant higher than that of WT mice.


CONCLUSIONS
Deletion of Mip-1alpha and CCR5 could reduce the MMP-9 expression in both macrophages and granulocytes, while deletion of CCR1 could reduce MMP-9 expression in macrophages but increase MMP-9 expression in granulocytes.
Wu
Yu
Y
Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Mukaida
Naofumi
N
Liu
Ting
T
Jia
Yong-qian
YQ
Zhu
Huan-ling
HL
Yang
Yi-ming
YM
Xu
Cai-gang
CG
Hu
Zhang-xue
ZX
D000818:Animals; D054405:Chemokine CCL3 / Q000235:genetics*; D005260:Female; D006098:Granulocytes / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054389:Receptors, CCR1 / Q000235:genetics*; D019713:Receptors, CCR5 / Q000235:genetics*
D054405:Chemokine CCL3; D012333:RNA, Messenger; D054389:Receptors, CCR1; D019713:Receptors, CCR5; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse