Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater.
A Gram-negative, pale-yellow, non-spore-forming bacterium, motile with single polar flagella, strain E6T, was isolated from coastal seawater collected from Tianjin, China, and its taxonomic position was investigated using a polyphasic approach. Strain E6T requires NaCl for growth and grows optimally at pH 8.2 and 36 degrees C and in the presence of 2.0% (w/v) NaCl. It is positive for catalase and oxidase, and reduces nitrate to nitrite. The major fatty acids (>10%) are C18:1omega7c (64.71%) and 11-methyl C18:1omega7c (12.38%), the ubiquinone system is Q-10 and the DNA G+C content is 53.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain E6T represented a new lineage in the Alphaproteobacteria and is related to genera Cucumibacter, Devosia, Ochrobactrum and Ahrensia. Strain E6T shows highest 16S rRNA gene sequence similarity (93.3%) to Cucumibacter marinus CL-GR60T and less than 92.2% similarity to other relatives. It can be differentiated from its closest phylogenetic neighbours on the basis of several phenotypic features, including nitrate reduction, assimilation of D-glucose, L-arabinose, D-mannose, mannitol and maltose and major fatty acid composition. A polyphasic analysis supported the conclusion that strain E6T represents a novel genus and species of the family Hyphomicrobiaceae, for which the name Zhangella mobilis gen. nov., sp. nov. is proposed. The type strain of Zhangella mobilis is E6T (=CGMCC 1.7002T=JCM 15144T).
Xu
Hai-Ying
HY
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Chen
Li-Ping
LP
Fu
Song-Zhe
SZ
Fan
Hong-Xia
HX
Zhou
Yu-Guang
YG
Liu
Shuang-Jiang
SJ
Liu
Zhi-Pei
ZP
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D041921:Hyphomicrobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008124:Locomotion; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase