title: Three distinct begomoviruses associated with soybean in central Brazil.
abstract: We report the complete nucleotide sequences of geminiviruses of the genus Begomovirus infecting soybean (Glycine max) in central Brazil. Samples obtained from soybean plants collected at Santo Antonio de Goiás, Goiás State, showing typical symptoms of viral infection, were analyzed. Infection was confirmed by PCR-based amplification of a DNA-A fragment with universal begomovirus primers. Total DNA from infected plants was then subjected to rolling-circle amplification (RCA), and 2.6-kb molecules were cloned into plasmid vectors. Sequencing of the three DNA-A and two DNA-B clones thus obtained confirmed infection by three distinct begomoviruses: bean golden mosaic virus, Sida micrantha mosaic virus and okra mottle virus, the last of which was reported recently to be a novel virus infecting okra plants in Brazil. Begomovirus infection of soybean plants has been reported sporadically in Brazil and has generally not been considered to be of economic relevance.
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Transferência de Genes, PqEB W5 Norte, Brasília, DF, 70770-900, Brazil.
authors.forename: Fernanda R
authors.initials: FR
authors.lastname: Fernandes
authors.forename: A R R
authors.initials: AR
authors.lastname: Cruz
authors.forename: J C
authors.initials: JC
authors.lastname: Faria
authors.forename: F M
authors.initials: FM
authors.lastname: Zerbini
authors.forename: Francisco J L
authors.initials: FJ
authors.lastname: Aragão
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D013025:Glycine max / Q000821:virology*
chemical_list: D004279:DNA, Viral