[Screening of target proteins interacting with dexamethasone derivates in vivo].
OBJECTIVE
The anti-tumor activity of dexamethasone derivatives (9-fluoro-16alpha-methyl-11,17-dihydroxy-3-oxo-1,4-androsladiene-17beta-carboxylic acid) is superior to that of dexamethasone. This study was to screen the proteins interacting with dexamethasone derivates, thus to explore the anti-tumor mechanism of dexamethasone derivates in vivo.


METHODS
The bait plasmid pGBKT7-GRalpha-LBD was constructed. Screening of the target proteins interacting with dexamethasone derivatives was performed by yeast three-hybrid technique using human K562 cell cDNA library.


RESULTS
The bait plasmid was successfully constructed. It produced a 31 ku bait protein with no toxicity, leakage and self-activation. Thirty-seven positive clones which interacted with dexamethasone derivatives were obtained from human K562 cell cDNA library, 20 of which were identified by re-transforming into yeast AH109 cells.


CONCLUSIONS
Twenty positive clones interacting with dexamethasone derivates are identified in vivo.
Hao
Po
P
Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Chongqing Medical University, Chongqing, P. R. China.
Wang
Chong
C
Liu
Bei-Zhong
BZ
Meng
Fan-Ping
FP
Wang
Dong-Sheng
DS
Wang
Chun-Guang
CG
Liu
Chang
C
Jin
Dan-Ting
DT
D000730:Androstadienes / Q000494:pharmacology*; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000494:pharmacology; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D020014:K562 Cells; D010957:Plasmids; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques
C558814:9-fluoro-16alpha-methyl-11,17-dihydroxy-3-oxo-1,4-androstadiene-17beta-carboxylic acid; D000730:Androstadienes; D018931:Antineoplastic Agents, Hormonal; D011993:Recombinant Fusion Proteins; D003907:Dexamethasone