[Effect of androsterone on scavenger receptor class B type I in human vascular endothelial cells].
OBJECTIVE
To investigate the effect of FXR on scavenger receptor class B type I (SR-BI) expression.


METHODS
Human vascular endothelium Eahy926 cells were treated with FXR agonist androsterone, and the specific target gene of FXR SHP mRNA was detected by RT-PCR. SR-BI mRNA and protein were determined using RT-PCR, real-time PCR and Western blotting.


RESULTS
The level of SHP mRNA in Eahy926 cells increased after androsterone treatment at different concentrations for 24 h, demonstrating FXR activation in the cells. RT-PCR, real-time PCR and Western blotting detected increased SR-BI expression at both mRNA and protein levels after FXR activation.


CONCLUSIONS
FXR increases the expression of SR-BI in human vascular endothelium cells.
Chao
Fan
F
Department of Biochemistry and Molecular Biology, Third Military Medical University, Chongqing 400038, China. fan620@gmail.com
Gong
Wei
W
Li
Yuan
Y
Xu
Zheng-Zhen
ZZ
Gao
Ming
M
He
Feng-Tian
FT
D000738:Androsterone / Q000494:pharmacology*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D018160:Receptors, Cytoplasmic and Nuclear / Q000819:agonists* / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000378:metabolism*; D000099150:Receptor, Farnesoid X-Activated
D000738:Androsterone; D018160:Receptors, Cytoplasmic and Nuclear; D051122:Scavenger Receptors, Class B; D000099150:Receptor, Farnesoid X-Activated; C495124:SCARB1 protein, human