title: [Neuronal mechanisms of associative food aversion conditioning reconsolidation in snail Helix lucorum].
abstract: We have previously showed that reactivation of long-term memory during protin synthesis inhibitor application initiated disruption of memory recalling in snails Helix lucorum with food aversion conditioning reflex. In present work cellular mechanisms of memory reactivation were studied in snail LP11 and RP11 command neurons of defense behavior. In first trial experiments mechanisms of amnesia induction were investigated in semiintact preparations 24 hours after aversion conditioning with single type of food. It was found that application of conditioned food stimulus on snail lip during CNS perfusion with cycloheximide (protein synthesis inhibitor) initiated depression of synaptic response evoked by conditioned stimulus 2.5 hours after reminding. In second tria experiments neuronal mechanisms of amnesia development were studied. Snails were conditioned with two types of food. Cycloheximide was injected into mantle cavity and conditioned stimulus of one type of food was presented 24 hours after snail learning. Semiintact preparations were prepared 1,3, 7 and 15 days after cycloheximide injection + reminding procedure. It was found that neural responses evoked by conditioned food stimulus which was used as reminding stimulus gradually decreased during 1, 3 and 7 days. Neural responses evoked by the conditioned stimulus at 7 and 15 days were not significantly differed from control differentiated food stimulus and were significantly weaker then neural responses evoked by second conditioned food stimulus which was not used as a reminding stimulus. It was suggested that specific and protein synthesis-dependent changes in synaptic connections effectiveness in LP11 and RP11 neurons is one of the cellular mechanisms of amnesia obtained after disruption of long-term memory reconsolidation in snail.
authors.forename: S A
authors.initials: SA
authors.lastname: Kozyrev
authors.forename: V P
authors.initials: VP
authors.lastname: Nikitin
mesh_terms: D000647:Amnesia / Q000503:physiopathology*; D000818:Animals; D001245:Association Learning / Q000187:drug effects / Q000502:physiology*; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D003214:Conditioning, Classical; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D005502:Food; D006372:Helix, Snails / Q000502:physiology*; D011939:Mental Recall / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D011500:Protein Synthesis Inhibitors