Predictive bioinformatic identification of minor receptor group human rhinoviruses.
Major group HRVs bind intercellular adhesion molecule 1 and minor group HRVs bind members of the low-density lipoprotein receptor (LDLR) family for cell entry. Whereas the former share common sequence motives in their viral capsid proteins (VPs), in the latter only a lysine residue within the binding epitope in VP1 is conserved; this lysine is also present in "K-type" major group HRVs that fail to use LDLR for infection. By using the available sequences three-dimensional models of VP1 of all HRVs were built and binding energies, with respect to module 3 of the very-low-density lipoprotein receptor, were calculated. Based on the predicted affinities K-type HRVs and minor group HRVs were correctly classified.
Weber
Christoph
C
Department of Medical Biochemistry, Medical University of Vienna, Vienna, Austria.
Pickl-Herk
Angela
A
Khan
Abdul Ghafoor
AG
Strauss
Sascha
S
Carugo
Oliviero
O
Blaas
Dieter
D
D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D012229:Rhinovirus / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D012984:Software; D053585:Virus Attachment
D036022:Capsid Proteins; D011973:Receptors, LDL