title: Complete genome sequences of two distinct and diverse Citrus tristeza virus isolates from New Zealand.
abstract: Two Citrus tristeza virus (CTV) isolates from New Zealand that display distinct phenotypes were isolated, examined and sequenced in full. The first isolate, NZ-M16, is largely asymptomatic and non-transmissible by the aphid vector Toxoptera citricida, while the second, NZ-B18, is highly transmissible and induces very severe symptoms on C. sinensis and C. aurantii. Phylogenetic analysis of the genome sequences showed that both isolates were approximately 90-93% similar to the VT and T318 isolates but possessed only 89% identity to one another. Based on sequence identity, both isolates are VT subtypes, with NZ-M16 being T3-like, while NZ-B18 is a member of a novel subtype with B165 from India.
authors.affiliation: School of Biological Sciences, University of Auckland, Auckland, 0630, New Zealand. shar127@aucklanduni.ac.nz
authors.forename: S J
authors.initials: SJ
authors.lastname: Harper
authors.forename: T E
authors.initials: TE
authors.lastname: Dawson
authors.forename: M N
authors.initials: MN
authors.lastname: Pearson
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D001483:Base Sequence; D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009520:New Zealand; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid