title: CE-MS method development for peptides analysis, especially hepcidin, an iron metabolism marker.
abstract: A method for the resolution of a peptides mixture including hepcidin-25, an iron metabolism marker, was developed by CE-ESI-MS. Several strategies were tested to optimize peptide separation, such as the addition of cyclodextrins or organic solvents in the BGE or the use of coated capillaries. Best results in terms of resolution, symmetry and efficiency were obtained with a BGE made of 500 mM ammonium acetate pH 4.5/ACN 70:30 v/v. Using the methodology of experimental design, BGE concentration, sheath liquid composition and MS-coupling parameters were then optimized in order to obtain the best signal intensity for hepcidin. Finally, a 225 mM BGE and a sheath liquid composed of isopropanol/water 80:20 v/v containing 0.5% v/v formic acid were selected as it constitutes the best compromise for selectivity, peak shape and sensitivity.
authors.affiliation: Department of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Liège, Belgium.
authors.forename: Gaëlle B
authors.initials: GB
authors.lastname: Martin
authors.forename: François
authors.initials: F
authors.lastname: Mansion
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Servais
authors.forename: Benjamin
authors.initials: B
authors.lastname: Debrus
authors.forename: Eric
authors.initials: E
authors.lastname: Rozet
authors.forename: Philippe
authors.initials: P
authors.lastname: Hubert
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000032:analysis*; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods*; D064451:Hepcidins; D006736:Horses; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015999:Multivariate Analysis; D009994:Osmolar Concentration; D010446:Peptide Fragments / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature
chemical_list: D000085:Acetates; D000097:Acetonitriles; D023181:Antimicrobial Cationic Peptides; C578702:HAMP protein, human; D064451:Hepcidins; D010446:Peptide Fragments; D011506:Proteins; C018824:ammonium acetate; C032159:acetonitrile