title: Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules.
abstract: A lipase from Thermomyces lanuginosus and cutinases from Thermobifida fusca and Fusarium solani hydrolysed poly(ethylene terephthalate) (PET) fabrics and films and bis(benzoyloxyethyl) terephthalate (3PET) endo-wise as shown by MALDI-Tof-MS, LC-UVD/MS, cationic dyeing and XPS analysis. Due to interfacial activation of the lipase in the presence of Triton X-100, a seven-fold increase of hydrolysis products released from 3PET was measured. In the presence of the plasticizer N,N-diethyl-2-phenylacetamide (DEPA), increased hydrolysis rates of semi-crystalline PET films and fabrics were measured both for lipase and cutinase. The formation of novel polar groups resulted in enhanced dye ability with additional increase in colour depth by 130% and 300% for cutinase and lipase, respectively, in the presence of plasticizer.
authors.affiliation: Research Centre Applied Biocatalysis, Petersgasse 14, A-8010 Graz, Austria.
authors.forename: Anita
authors.initials: A
authors.lastname: Eberl
authors.forename: Sonja
authors.initials: S
authors.lastname: Heumann
authors.forename: Tina
authors.initials: T
authors.lastname: Brückner
authors.forename: Rita
authors.initials: R
authors.lastname: Araujo
authors.forename: Artur
authors.initials: A
authors.lastname: Cavaco-Paulo
authors.forename: Franz
authors.initials: F
authors.lastname: Kaufmann
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D000083:Acetanilides / Q000494:pharmacology; D001565:Benzoates / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005670:Fusarium / Q000201:enzymology; D006868:Hydrolysis / Q000187:drug effects; D008049:Lipase / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism*; D011093:Polyethylene Terephthalates / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties / Q000187:drug effects
chemical_list: D000083:Acetanilides; D001565:Benzoates; D010795:Phthalic Acids; D011093:Polyethylene Terephthalates; C546855:bis(benzoyloxyethyl)terephthalate; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D008049:Lipase; C044250:N,N-diethylphenylacetamide