title: Main chain NMR assignments of subtilisin Sbt70 in its prodomain-bound state.
abstract: Main chain assignments are described for a 266-residue subtilisin mutant, Sbt70, in its 35 kDa complex with an N-terminal prodomain. The assignments provide the basis for understanding how the prodomain assists folding of subtilisin at a residue-specific level.
authors.affiliation: Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA.
authors.forename: Nese
authors.initials: N
authors.lastname: Sari
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Fisher
authors.forename: Philip N
authors.initials: PN
authors.lastname: Bryan
authors.forename: John
authors.initials: J
authors.lastname: Orban
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008970:Molecular Weight; D009587:Nitrogen Isotopes / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011522:Protons; D020860:Subtilisin / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D020860:Subtilisin