Anti-lipase and lipolytic activities of ursolic acid isolated from the roots of Actinidia arguta.
The aim of this study was to investigate the anti-obestic effects of ursolic acid isolated from the roots of Actinidia arguta, as well as the mechanism of action of this compound. This was conducted by testing whether ursolic acid inhibited the elevation of the rat plasma triacylglycerol levels after oral administration of a lipid emulsion containing corn oil in rats. Ursolic acid prevented the elevation of plasma triacylglycerol levels 2 h after oral administration of the lipid emulsion at a dose of 100 mg/kg. Furthermore, ursolic acid inhibited phosphodiesterase activity in vitro with an IC(50) of 51.21 muM and enhanced lipolysis in rat fat cells. We suggest that the inhibitory effects of ursolic acid, isolated from the roots of A. arguta, on obesity, might be attributable to the inhibition of lipid absorption through the inhibition of pancreatic lipase and by enhancing lipolysis in fat cells.
Kim
Junghyun
J
Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Daejeon, 305-811, Korea.
Jang
Dae Sik
DS
Kim
Hyojun
H
Kim
Jin Sook
JS
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D029042:Actinidia / Q000737:chemistry; D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology; D000284:Administration, Oral; D000818:Animals; D019440:Anti-Obesity Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003314:Corn Oil / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D007783:Lactones / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D000077403:Orlistat; D010726:Phosphodiesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000097245:Ursolic Acid
D015056:1-Methyl-3-isobutylxanthine; D019440:Anti-Obesity Agents; D003314:Corn Oil; D007783:Lactones; D008049:Lipase; D000077403:Orlistat; D010726:Phosphodiesterase Inhibitors; D014280:Triglycerides; D014315:Triterpenes; D000097245:Ursolic Acid