Solid-phase peptide synthesis using acetonitrile as a solvent in combination with PEG-based resins.
This manuscript shows that ACN can be an excellent choice for the coupling of hindered amino acids as illustrated by the coupling of Fmoc-amino acids on free amino acids anchored on a BAL synthesis. Furthermore, ACN can be a good alternative for solid-phase peptide synthesis in the absence of DMF (washings, removal of Fmoc, and coupling).
Acosta
Gerardo A
GA
CIBER-BBN, Networking Centre on Bioengineering Biomaterials and Nanomedicine, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain.
del Fresno
Montserrat
M
Paradis-Bas
Marta
M
Rigau-DeLlobet
Maria
M
Côté
Simon
S
Royo
Miriam
M
Albericio
Fernando
F
D000097:Acetonitriles / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012997:Solvents
D000097:Acetonitriles; D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides; D011137:Polystyrenes; D012997:Solvents; C517863:aminomethyl-ChemMatrix resin; C103390:polyethylene glycol-polystyrene; D011092:Polyethylene Glycols; C032159:acetonitrile