Voltage-dependent anion channels 1 and 2 are expressed in porcine oocytes.
The eukaryotic VDAC (voltage-dependent anion channel) is a pore-forming protein originally discovered in the outer membrane of mitochondria. It has been established as a key player in mitochondrial metabolism and ion signalling. In addition, in recent years, it has also been proposed that VDAC is present in extra-mitochondrial membranes, and it has been related to cytoskeletal structures. However, little is known about the presence and intracellular localization of VDAC subtypes in mammalian gametes. In the present study, we confirm the synthesis of VDAC1 and 2 subtypes in GV (germinal vesicle) and MII (meiosis II) stage porcine oocytes as well as their protein expression. A shift in the abundance of immunoreactive 32 kDa VDAC protein between GV and MII stage oocytes was observed with anti-VDAC2 antibody. Furthermore, subcellular localization by confocal laser microscopy demonstrated fluorescent labelling of VDAC1 over the entire oocyte surface, suggesting the presence of VDAC1 in the porcine oocyte plasma membrane and around the cortical area. Anti-VDAC2 immunostaining yielded ring-like clusters of structures distributed on the cortical area in some GV, but not in MII, stage oocytes. These results are the first data obtained for VDAC in mammalian female gametes and provide the basis for studying protein-protein interactions, distribution and possible functions of VDAC subtypes during maturation and fertilization of mammalian oocytes.
Cassará
María Carolina
MC
Department of Urology, Pediatric Urology and Andrology, Justus Liebig University of Giessen, Rudolf-Buchheim-Strasse 7, Germany. carolina.cassara@yahoo.com
Menzel
Viviana Andrea
VA
Hinsch
Klaus-Dieter
KD
Wrenzycki
Christine
C
Hinsch
Elvira
E
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015151:Immunoblotting; D007150:Immunohistochemistry; D008540:Meiosis; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D050995:Voltage-Dependent Anion Channel 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050996:Voltage-Dependent Anion Channel 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D050996:Voltage-Dependent Anion Channel 2; D050995:Voltage-Dependent Anion Channel 1