title: [Study for drug-resistance of epithelial ovarian cancer by serum protein profiling].
abstract: OBJECTIVE
  To develop the rationales for ovarian cancer-specific protein profiles in serum and to analyze the protein profiles of multidrug resistance P-glucoprotein (MDR1) and multidrug resistance-associated protein (MRP) positive and negative expression sets for a rapid and sensitive serum protein pattern.
  
  
  METHODS
  Serum protein profiles from 36 epithelial ovarian cancer cases were compared with 30 healthy controls using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (DELDI-TOF-MS). Blinded test was conducted subsequently for identification of the protein pattern. Expression of MDR and MRP were detected in set of training cases by immunohistochemistry. The spectra were analyzed statistically between positive and negative groups.
  
  
  RESULTS
  Twenty-nine peaks were significantly different between ovarian cancer and healthy controls (P < 0.01, 15 peaks up-regulated and 14 down-regulated). A set of three peaks, at 5 486, 6 463 and 8 575 m/z respectively, were selected from 29 sense peaks as an ovarian cancer biomarker. The sensitivity was 100% and specificity 93.33%. Identification by blinded validation indicated a positive predictive value of 90%. MDR1 expression in ovarian cancer was 69.4 %. Twenty peaks were significantly different between positive and negative sets (P < 0.01). MRP expression in ovarian cancer was 63.8%. But one peak was detected (P < 0.01).
  
  
  CONCLUSIONS
  It was an ideal pattern for employing the SELDI mass spectrum approach to diagnose ovarian cancer and select the multidrug resistance cases. Serum biomarkers for detecting drug resistance in ovarian cancer can be established on the basis of MDR1 immunohistochemistry.
authors.affiliation: Department of Ob/Gyn, No. 306 PLA Hospital, Beijing 100101, China.
authors.forename: Wen-ying
authors.initials: WY
authors.lastname: Zhang
authors.forename: Li-rong
authors.initials: LR
authors.lastname: Zhu
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Zheng
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Jiu-hong
authors.initials: JH
authors.lastname: Wu
authors.forename: Qin-ping
authors.initials: QP
authors.lastname: Liao
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000097:blood*; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D010449:Peptide Mapping; D040901:Proteomics
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D014408:Biomarkers, Tumor; D001798:Blood Proteins