Multilocus sequence typing of Campylobacter jejuni, and the correlation between clonal complex and pulsed-field gel electrophoresis macrorestriction profile.
The characterization of Campylobacter jejuni has been significantly improved by the use of multilocus sequence typing (MLST), which allows the relationship between isolates to be determined. The sequence types (STs) of 261 isolates of C. jejuni from New Zealand were determined. Isolates were obtained from a range of sources including chicken meat, cattle, pigs, duck, sheep, water and human infections. Thirty-two new alleles and 44 new STs were identified. Comparison of the MLST data and pulsed-field gel electrophoresis macrorestriction profiles showed that the macrorestriction profiles were good predictors of the clonal complex (CC) but not ST. All the major CCs identified elsewhere in the world were found in New Zealand as well as the association of certain CCs with particular animal niches. The majority of new STs identified were from river water isolates.
McTavish
Sharla M
SM
ESR Ltd, Kenepuru Science Centre, Porirua, New Zealand.
Pope
Christopher E
CE
Nicol
Carolyn
C
Campbell
Donald
D
French
Nigel
N
Carter
Philip E
PE
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics*; D002417:Cattle; D002645:Chickens; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004372:Ducks; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D009520:New Zealand; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods*; D012756:Sheep; D013223:Statistics as Topic; D013552:Swine