Tubulin polymerization-promoting protein (TPPP/p25) is critical for oligodendrocyte differentiation.
TPPP/p25, a recently identified tubulin polymerization-promoting protein (TPPP), is expressed mainly in myelinating oligodendrocytes of the CNS. Here, we show that TPPP/p25 is strongly upregulated during the differentiation of primary oligodendrocyte cells as well as the CG-4 cell line. The microRNA expression profile of CG-4 cells before and after induction of differentiation was established and revealed differential regulation of a limited subset of microRNAs. miR-206, a microRNA predicted to target TPPP/p25, was not detected in oligodendrocytes. Overexpression of miR-206 led to downregulation of TPPP/p25 resulting in inhibition of differentiation. Transfection of siRNAs against TPPP/p25 also inhibited cell differentiation and promoted cell proliferation, providing evidence for an important role of TPPP/p25 during oligodendrogenesis. These results support an essential role for TPPP/p25 in oligodendrocyte differentiation likely via rearrangement of the microtubule system during the process elongation prior to the onset of myelination.
Lehotzky
Attila
A
Institute of Enzymology, Biological Research Centre, Hungarian Academy of Sciences, H-1113 Budapest, Hungary.
Lau
Pierre
P
Tokési
Natália
N
Muja
Naser
N
Hudson
Lynn D
LD
Ovádi
Judit
J
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism; D008870:Microtubules / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012689:Sequence Homology, Nucleic Acid; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D013997:Time Factors; D015854:Up-Regulation
D002352:Carrier Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C547161:TPPP protein, mouse; C539090:TPPP protein, rat