Shotgun proteomics in neuroscience.
Mass spectrometry-based proteomics is increasingly used to address basic and clinical questions in biomedical research through studies of differential protein expression, protein-protein interactions, and posttranslational modifications. The complex structural and functional organization of the human brain warrants the application of high-throughput, systematic approaches to understand the functional alterations under normal physiological conditions and the perturbations of neurological diseases. This primer focuses on shotgun-proteomics-based tandem mass spectrometry for the identification of proteins in a complex mixture. It describes the basic concepts of protein differential expression analysis and posttranslational modification analysis and discusses several strategies to improve the coverage of the proteome.
Liao
Lujian
L
Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
McClatchy
Daniel B
DB
Yates
John R
JR
D000818:Animals; D001921:Brain / Q000378:metabolism*; D030562:Databases, Protein; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009488:Neurosciences / Q000379:methods*; D025941:Protein Interaction Mapping; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D020543:Proteome