title: [Post vaccination rotavirus surveillance in Hungary, in 2007].
abstract: Vaccination is the main strategy to control severe dehydrating gastroenteritis caused by rotaviruses in early childhood. The availability of new generation rotavirus vaccines has led to an intensification of strain surveillance worldwide, in part, to gauge the impact of the possible vaccine-driven immune selection of wild-type rotavirus strains. In the present study, authors describe the strain prevalence data obtained in 2007, with the involvement of different regions of Hungary. Genomic RNA was extracted from rotavirus-positive stool samples collected mainly from children and then subjected to genotyping using multiplex RT-PCR assay. Type-specific primers targeted G1 to G4, G6, G8 to G10, and G12 VP7 specificities, and P[4], P[6], and P[8] to P[11] VP4 specificities were used. Out of 489 rotavirus-positive specimens, collected from 482 patients, 466 and 474 were successfully G and P typed, respectively, and both G and P type specificities could be assigned for 457 strains. Prevalence data showed the predominance of G4P[8] (31.5%) strains, followed by G1P[8] (28.3%), G2P[4] (19.3%), and G9P[8] (10.2%). Minority strains were G1P[4] (0.4%), G2P[8] (1.3%), G3P[9] (0.2%), G4P[6] (0.7%), G6P[9] (0.4%), G8P[8] (0.2%), G9P[4] (0.2%), G9P[6] (0.8%), and G12P[8] (0.4%). Mixed infections were found in 1.2% of the samples, while 4.9% remained partially or fully non-typified. Our data indicate that the antigen specificities of medically important rotavirus strains identified in this 1-year study are well represented in the vaccines available in the pharmaceutical private market in Hungary. Depending on the vaccination coverage achievable in the forthcoming years, the post-vaccination rotavirus strain surveillance may allow us to gain comprehensive information on the impact of rotavirus vaccines on the prevalence of circulating rotavirus strains.
authors.affiliation: Debreceni Egyetem, Altalános Orvostudományi Kar, Orvos- és Egészségtudományi Centrum Orvosi Mikrobiológiai Intézet, Debrecen.
authors.forename: Brigitta
authors.initials: B
authors.lastname: László
authors.forename: Edina
authors.initials: E
authors.lastname: Czellár
authors.forename: Judit
authors.initials: J
authors.lastname: Deák
authors.forename: Agnes
authors.initials: A
authors.lastname: Juhász
authors.forename: Julianna
authors.initials: J
authors.lastname: Kovács
authors.forename: József
authors.initials: J
authors.lastname: Kónya
authors.forename: Júlia
authors.initials: J
authors.lastname: Mészáros
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Mészner
authors.forename: Ilona
authors.initials: I
authors.lastname: Mihály
authors.forename: Péter
authors.initials: P
authors.lastname: Molnár
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Nyúl
authors.forename: László
authors.initials: L
authors.lastname: Pátri
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Puskás
authors.forename: Ferenc
authors.initials: F
authors.lastname: Schneider
authors.forename: Csaba
authors.initials: C
authors.lastname: Siffel
authors.forename: András
authors.initials: A
authors.lastname: Tóth
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Tóth
authors.forename: György
authors.initials: G
authors.lastname: Szucs
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
mesh_terms: D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000517:prevention & control*; D022243:Rotavirus Vaccines / Q000008:administration & dosage*; D012621:Seasons
chemical_list: D012367:RNA, Viral; D022243:Rotavirus Vaccines