Effect of nanoparticulate bioactive glass particles on bioactivity and cytocompatibility of poly(3-hydroxybutyrate) composites.
This work investigated the effect of adding nanoparticulate (29 nm) bioactive glass particles on the bioactivity, degradation and in vitro cytocompatibility of poly(3-hydroxybutyrate) (P(3HB)) composites/nano-sized bioactive glass (n-BG). Two different concentrations (10 and 20 wt %) of nanoscale bioactive glass particles of 45S5 Bioglass composition were used to prepare composite films. Several techniques (Raman spectroscopy, scanning electron microscopy, atomic force microscopy, energy dispersive X-ray) were used to monitor their surface and bioreactivity over a 45-day period of immersion in simulated body fluid (SBF). All results suggested the P(3HB)/n-BG composites to be highly bioactive, confirmed by the formation of hydroxyapatite on material surfaces upon immersion in SBF. The weight loss and water uptake were found to increase on increasing bioactive glass content. Cytocompatibility study (cell proliferation, cell attachment, alkaline phosphatase activity and osteocalcin production) using human MG-63 osteoblast-like cells in osteogenic and non-osteogenic medium showed that the composite substrates are suitable for cell attachment, proliferation and differentiation.
Misra
Superb K
SK
Department of Materials, Imperial College London, London SW7 2BP, UK.
Ansari
Tahera
T
Mohn
Dirk
D
Valappil
Sabeel P
SP
Brunner
Tobias J
TJ
Stark
Wendelin J
WJ
Roy
Ipsita
I
Knowles
Jonathan C
JC
Sibbons
Paul D
PD
Jones
Eugenia Valsami
EV
Boccaccini
Aldo R
AR
Salih
Vehid
V
D001672:Biocompatible Materials; D001709:Biotechnology; D001826:Body Fluids / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry*; D005898:Glass; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D011091:Polyesters; C425330:bioactive glass 45S5; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates