title: Dietary management of hepatic copper accumulation in Labrador Retrievers.
abstract: BACKGROUND
  Copper-associated chronic hepatitis (CACH) recently has been recognized in the Labrador Retriever as an inherited disorder with a late onset of clinical signs. No studies have investigated dietary management for the long-term treatment of this disease or for its potential in delaying the onset of clinical signs in subclinical cases.
  
  
  OBJECTIVE
  To investigate the effects of a low-copper diet and zinc gluconate on hepatic copper concentrations in Labrador Retrievers with abnormal hepatic copper concentrations.
  
  
  METHODS
  Twenty-four client-owned Labradors that were related to patients affected with CACH and that had been diagnosed with increased hepatic copper concentrations.
  
  
  METHODS
  Hepatic copper concentrations were assessed before and after an average of 8 and 16 months of treatment. During this time, all dogs were fed exclusively a low-copper diet. In addition, dogs were assigned to 1 of 2 groups in a randomized double-blind manner to receive a supplement of zinc gluconate or placebo.
  
  
  RESULTS
  Twenty-one dogs completed the study. Hepatic copper concentrations decreased in both groups at recheck 1 (n = 21; group 1, P < .001; group 2, P= .001) and at recheck 2 (n= 16; group 1, P= .03; group 2, P= .04). No difference in hepatic copper concentrations was found between the 2 groups before treatment (P= .65), at recheck 1 or at recheck 2 (P= .52-.79).
  
  
  CONCLUSIONS
  Feeding low-copper diets to Labradors is effective in decreasing hepatic copper concentrations. Adjunctive treatment with zinc does not appear to increase the copper-lowering effects of dietary management.
authors.affiliation: Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, P.O. box 80.154, 3508 TD, The Netherlands. g.hoffmann@uu.nl
authors.forename: G
authors.initials: G
authors.lastname: Hoffmann
authors.forename: P G
authors.initials: PG
authors.lastname: Jones
authors.forename: V
authors.initials: V
authors.lastname: Biourge
authors.forename: T S G A M
authors.initials: TS
authors.lastname: van den Ingh
authors.forename: S J
authors.initials: SJ
authors.lastname: Mesu
authors.forename: P
authors.initials: P
authors.lastname: Bode
authors.forename: J
authors.initials: J
authors.lastname: Rothuizen
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D056487:Chemical and Drug Induced Liver Injury, Chronic / Q000178:diet therapy / Q000378:metabolism / Q000662:veterinary*; D003300:Copper / Q000008:administration & dosage / Q000378:metabolism*; D004283:Dog Diseases / Q000178:diet therapy* / Q000378:metabolism*; D004285:Dogs; D004311:Double-Blind Method; D005260:Female; D020022:Genetic Predisposition to Disease; D005942:Gluconates / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006520:Hepatitis, Animal / Q000139:chemically induced* / Q000178:diet therapy; D006651:Histocytochemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male
chemical_list: D005942:Gluconates; D003300:Copper; C030691:gluconic acid