title: NMR resonance assignment of DnaE intein from Nostoc punctiforme.
abstract: DnaE intein from Nostoc punctiforme (Npu) is one of naturally occurring split inteins, which has robust protein splicing activity. Highly efficient trans-splicing activity of NpuDnaE intein could widen various biotechnological applications. However, structural basis of the efficient protein splicing activity is poorly understood. As a first step toward better understanding of protein trans-splicing mechanism, we present the backbone and side-chain resonance assignments of a single chain variant NpuDnaE intein as determined by triple resonance experiments with [(13)C,(15)N]-labeled protein.
authors.affiliation: Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, Helsinki, 00014, Finland.
authors.forename: Kimmo
authors.initials: K
authors.lastname: Heinämäki
authors.forename: Jesper S
authors.initials: JS
authors.lastname: Oeemig
authors.forename: Kimmo
authors.initials: K
authors.lastname: Pääkkönen
authors.forename: Janica
authors.initials: J
authors.lastname: Djupsjöbacka
authors.forename: Hideo
authors.initials: H
authors.lastname: Iwaï
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D004258:DNA Polymerase III / Q000737:chemistry*; D047668:Inteins; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000737:chemistry; D046937:Nostoc / Q000737:chemistry*; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III