Gene expression profiling of monocytes displaying herpes simplex virus 1 induced dysregulation of antifungal defences.
Recently, we showed that herpes simplex virus 1 (HSV-1)-infected monocytes have altered antifungal defences, in particular they show augmented phagocytosis of Candida albicans followed by a failure of the intracellular killing of the ingested fungi. On the basis of these functional data, comparative studies were carried out on the gene expression profile of cells infected with HSV-1 and/or C. albicans in order to investigate the molecular mechanisms underlying such virus-induced dysfunction. Affymetrix GeneChip technology was used to evaluate the cell transcription pattern, focusing on genes involved in phagocytosis, fungal adhesion, antimicrobial activity and apoptosis. The results indicated there was: (a) prevalent inhibition of opsonin-mediated phagocytosis, (b) upregulation of several pathways of antibody- and complement-independent phagocytosis, (c) inhibition of macrophage activation, (d) marked dysregulation of oxidative burst, (e) induction of apoptosis.
Cermelli
Claudio
C
Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
Orsi
Carlotta Francesca
CF
Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
Cuoghi
Alessandro
A
Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
Ardizzoni
Andrea
A
Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
Tagliafico
Enrico
E
Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
Neglia
Rachele
R
Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
Peppoloni
Samuele
S
Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
Blasi
Elisabetta
E
Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002176:Candida albicans / Q000472:pathogenicity; D002448:Cell Adhesion / Q000235:genetics; D002460:Cell Line; D002522:Chlorocebus aethiops; D020869:Gene Expression Profiling; D018259:Herpesvirus 1, Human / Q000472:pathogenicity* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology / Q000502:physiology; D006801:Humans; D008262:Macrophage Activation / Q000235:genetics; D009000:Monocytes / Q000276:immunology / Q000473:pathology / Q000502:physiology* / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D009895:Opsonin Proteins / Q000502:physiology; D010587:Phagocytosis / Q000235:genetics; D016897:Respiratory Burst / Q000235:genetics; D014709:Vero Cells
D009895:Opsonin Proteins