Detection of Cucumber mosaic virus and typing using serological and molecular methods in Razavi Khorasan Province.
Investigation was conducted during 2006 and 2007 to detect and determine typing of Cucumber mosaic virus (CMV) in field-collected samples from Razavi Khorasan Province. Leaves showing chlorosis, mosaic, distortion and shoestring symptoms were collected from the Mashhad Regions (Astan-e-ghods and Khaje-rabie), Neishabour, Torbat-e-heidarye, Ghochan, Chenaran, Fariman, Shirvan, Kalat and Kashmar. Samples were transferred to laboratory in order to detect the virus in the collected samples, Samples were tested by DAS-ELISA. Typing was done for those serologically positive-reacted samples by RT-PCR-RFLP. Specific primers have amplified 650 bp fragments of RNA2, in RT-PCR assay. Digestion was performed by restriction enzyme MluI. Present research showed that all of collected samples were in subgroup IA.
Golnaz
N
N
Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran.
Jafarpour
B
B
Rastegar
M F
MF
Sabokkhiz
M A
MA
D017799:Cucumovirus / Q000235:genetics / Q000302:isolation & purification; D019662:Cucurbitaceae / Q000033:anatomy & histology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D007492:Iran; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D012698:Serologic Tests / Q000379:methods*
D004279:DNA, Viral