title: [Identification of the interactions between JTV1 and NLS-RAR alpha in vivo and in vitro].
abstract: OBJECTIVE
  To identify the interactions between JTV1 and NLS-RAR alpha by Yeast two-hybrid and co-immunoprecipitation.
  
  
  METHODS
  The plasmids of bait-protein and JTV1 protein were cotransformed into yeast AH109 to investigate their interactions in vivo. Tagged fusion protein eukaryotic expression vectors were constructed and then cotransfected into human embryo kidney 293 cells. Co-immunoprecipitation was used to investigate the interactions between NLS-RAR alpha and JTV1 in vitro.
  
  
  RESULTS
  Blue clones were found in QDO/X-alpha-gal plates. Eukaryotic expression vectors were co-transfected into HEK 293 cells. The HA-NLS-RAR alpha protein was immunoprecipitated by anti-HA polyclonal antibody. Myc-JTV1 protein was detected by western blotting with anti-Myc monoclonal antibody from the immunoprecipitared complex.
  
  
  CONCLUSIONS
  The interactions between NLS-RAR alpha and JTV1 are identified by Yeast two-hybrid and co-immunoprecipitation.
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Faculty of Laboratory Medicine in Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Bei-zhong
authors.initials: BZ
authors.lastname: Liu
authors.forename: Po
authors.initials: P
authors.lastname: Hao
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Dan-ting
authors.initials: DT
authors.lastname: Jin
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
authors.forename: Chun-guang
authors.initials: CG
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D000072482:Retinoic Acid Receptor alpha; D014162:Transfection; D020798:Two-Hybrid System Techniques
chemical_list: D009363:Neoplasm Proteins; C000607071:RARA protein, human; D018168:Receptors, Retinoic Acid; D011993:Recombinant Fusion Proteins; D000072482:Retinoic Acid Receptor alpha