Endothelial colony-forming cells from patients with chronic myeloproliferative disorders lack the disease-specific molecular clonality marker.
Two putative types of circulating endothelial progenitor cells have been recently identified in vitro: (1) endothelial colony-forming cell (ECFC) and (2) colony-forming unit-endothelial cell (CFU-EC). Only the former is now recognized to belong to endothelial lineage. We have used the ECFC and CFU-EC assays to readdress the issue of the clonal relation between endothelial progenitor cells and hematopoietic stem cells in patients with Philadelphia-positive and Philadelphia-negative chronic myeloproliferative disorders. Both ECFCs and CFU-ECs were cultured from peripheral blood mononuclear cells, and either BCR-ABL rearrangement or JAK2-V617F mutation were assessed in both types of endothelial colonies. We found that ECFCs lack the disease-specific markers, which are otherwise present in CFU-ECs, thus reinforcing the concept that the latter belongs to the hematopoietic lineage, and showing that in chronic myeloproliferative disorders the cell that gives rise to circulating ECFC has a distinct origin from the cell of the hematopoietic malignant clone.
Piaggio
Giovanna
G
Divisione Ematologia, Ospedale San Martino, Genova.
Rosti
Vittorio
V
Corselli
Mirko
M
Bertolotti
Francesca
F
Bergamaschi
Gaetano
G
Pozzi
Sarah
S
Imperiale
Davide
D
Chiavarina
Barbara
B
Bonetti
Elisa
E
Novara
Francesca
F
Sessarego
Mario
M
Villani
Laura
L
Garuti
Anna
A
Massa
Margherita
M
Ghio
Riccardo
R
Campanelli
Rita
R
Bacigalupo
Andrea
A
Pecci
Alessandro
A
Viarengo
Gianluca
G
Zuffardi
Orsetta
O
Frassoni
Francesco
F
Barosi
Giovanni
G
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002478:Cells, Cultured; D002908:Chronic Disease; D003114:Colony-Forming Units Assay; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000172:deficiency* / Q000235:genetics; D015321:Gene Rearrangement; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D053614:Janus Kinase 2 / Q000172:deficiency* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D009196:Myeloproliferative Disorders / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D013234:Stem Cells / Q000473:pathology*
D014408:Biomarkers, Tumor; D016044:Fusion Proteins, bcr-abl; C507924:JAK2 protein, human; D053614:Janus Kinase 2