title: [Adaptive changes of ultrastructures of dentritic cells during maturation induced by lipopolysaccharide].
abstract: OBJECTIVE
  To investigate the adaptive changes of ultrastructure of the dentritic cells (DC) before and after maturation.
  
  
  METHODS
  The murine bone marrow mononuclear cells were induced into immatured dendritic cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). The cell morphology was observed under an inverted phase contrast microscope, and the surface markers were detected by flow cytometry. Then the DC was induced to be matured with lipopolysaccharide (LPS) for 48 hours, the ultrastructures of DC was observed before and after maturation under transmission electron microscope and then a comparative analysis was doned.
  
  
  RESULTS
  The surface processes of matured dentritic cells stimulated by LPS decreased significantly, whereas the organelles and the diameter of nucleolus increased.
  
  
  CONCLUSIONS
  The distribution of surface processes may be associated with the antigen-presenting capacity of DC, and it is also a potential ruler of cell function and status.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Chongqing Medical University, Chongqing 400015, China.
authors.forename: De-Ping
authors.initials: DP
authors.lastname: Luo
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Dai-Qing
authors.initials: DQ
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Ji
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Qiu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015847:Interleukin-4; D008070:Lipopolysaccharides; D051379:Mice
chemical_list: D008070:Lipopolysaccharides; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor