Coordinated control of a designed trans-acting ligase ribozyme by a loop-receptor interaction.
We previously developed a synthetic cis-acting RNA ligase ribozyme with 3'-5' joining activity termed "DSL" (designed and selected ligase). DSL was easily transformed into a trans-acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans-acting DSL, tDSL-1/GUAA. tDSL-1/GUAA exhibited remarkably high activity compared with the parental cis-acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop-receptor interaction plays a significant role in determining the activity of the trans-acting ribozyme and in its ability to perform multiple turnovers of the reaction.
Matsumura
Shigeyoshi
S
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan.
Ohmori
Rei
R
Saito
Hirohide
H
Ikawa
Yoshiya
Y
Inoue
Tan
T
D020029:Base Pairing; D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011130:RNA Ligase (ATP) / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D016337:RNA, Catalytic; D011130:RNA Ligase (ATP)