Algoriphagus olei sp. nov., isolated from oil-contaminated soil.
A motile, Gram-negative, pinkish red-pigmented bacterium (strain CC-Hsuan-617(T)) was isolated from an oil-contaminated soil near an oil refinery located in Kaohsiung County, Taiwan. 16S rRNA gene sequence analysis showed that strain CC-Hsuan-617(T) clustered with Algoriphagus mannitolivorans (97.5 % sequence similarity), Algoriphagus aquatilis (98.0 %) and Algoriphagus boritolerans (97.5 %), followed by Algoriphagus ornithinivorans (97.1 %) and Algoriphagus alkaliphilus (97.1 %). The fatty acid profile of the novel strain was slightly different from those reported for recognized Algoriphagus species. The quinone system contained menaquinone MK-7 as the predominant component. The major polar lipids were phosphatidylethanolamine, an unknown polar lipid, an unknown phospholipid and an unknown aminophospholipid. The main cell polyamine was sym-homospermidine; lesser amounts of spermine and spermidine were also found. The results of DNA-DNA hybridization, and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain CC-Hsuan-617(T) from recognized Algoriphagus species. Strain CC-Hsuan-617(T) is thus considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus olei sp. nov. is proposed. The type strain is CC-Hsuan-617(T) (=BCRC 17886(T)=CCUG 57471(T)).
Young
Chiu-Chung
CC
Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
Lin
Shi-Yao
SY
Arun
A B
AB
Shen
Fo-Ting
FT
Chen
Wen-Ming
WM
Rekha
P D
PD
Langer
Stefan
S
Busse
Hans-Jürgen
HJ
Wu
Yun-Hsuan
YH
Kämpfer
Peter
P
D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004787:Environmental Pollution; D008969:Molecular Sequence Data; D009821:Oils / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis; D013624:Taiwan
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009821:Oils; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants