title: Molecular method for the diagnosis of imported pediatric malaria.
abstract: Malaria is a polymorphous disease; it can be life threatening especially for children. We report a case of imported malaria in a boy, illustrating the epidemiological and clinical aspects of severe pediatric malaria. In this case real-time PCR was used to quantify Plasmodium falciparum DNA levels, to monitor the evolution under treatment, and to determine genetic mutations involved in chloroquine resistance. The major epidemiological features of imported malaria, and the difficulty to diagnose childhood severe malaria are described. The contribution of molecular methods for the diagnosis of imported malaria is discussed.
authors.affiliation: Laboratoire de parasitologie-mycologie EA3609, département de microbiologie de médecine, faculté de médecine, université de Lille 2, CHU de Lille, Lille, France. l-delhaes@chru-lille.fr
authors.forename: L
authors.initials: L
authors.lastname: Delhaes Jeanne
authors.forename: A
authors.initials: A
authors.lastname: Berry
authors.forename: E
authors.initials: E
authors.lastname: Dutoit
authors.forename: F
authors.initials: F
authors.lastname: Leclerc
authors.forename: J
authors.initials: J
authors.lastname: Beaudou
authors.forename: S
authors.initials: S
authors.lastname: Leteurtre
authors.forename: D
authors.initials: D
authors.lastname: Camus
authors.forename: F
authors.initials: F
authors.lastname: Benoit-Vical
mesh_terms: D002648:Child; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D025202:Molecular Diagnostic Techniques; D016133:Polymerase Chain Reaction