[Metabolism of Escherichia coli is interfered by Bacillus subtilis glnA gene].
A recombinant strain Escherichia coli DH5alpha(pMD19-glnA) including Bacillus subtilis glnA gene was constructed. Capillary electrophoresis and nuclear magnetic resonance were used to determine qualitatively the product of transformation by recombinant strain, and the relative level of mRNA expression of glnA was also determined by fluorescence quantitative RT-PCR. Subsequently, SDS-PAGE (polyacrylamide gel electrophoresis) was used to analysis the relative level of protein. Surprisingly, there was no increase of glutamine production in this recombinant strain, but an obvious increase in the GABA (gamma-aminobutyric acid ) production. It was showed in the experiment that protein expression of the glutamine synthetase did not increase, although glnA gene can be transcribed normally in this recombined strain. The phenomenon of exogenous glnA gene interfering metabolism of Escherichia coli was worthy of further study.
Du
Juan
J
Department of Biotechnology, Zhengzhou University, Zhengzhou 450001, China.
Wu
Jian
J
Dai
Guifu
G
Wang
Chunyang
C
Zhou
Xinqin
X
Song
Minghui
M
Li
Jue
J
Li
Jilun
J
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis
D001426:Bacterial Proteins; D012333:RNA, Messenger; D005680:gamma-Aminobutyric Acid; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase