title: Microvalves actuated sandwich immunoassay on an integrated microfluidic system.
abstract: In this study, we developed an integrated and automatic microfluidic immunoassay system, which comprised a multi-layer immunoassay microfluidic chip and a rotary confocal LIF scanner. The normally closed elastomeric microvalves were integrated on the multi-layer microfluidic chip to realize flexible reagents delivery for the rapid immunoassay. The various operations of immunoassay including coating, blocking and washing could be performed on a single chip with reduced reagents consumption and analysis time. The homemade rotary LIF scanner was able to detect the signals from the multichannels sequentially in a short period of time, enabling the potential capability for high-throughput immunoassay. The performance of this system was demonstrated by the sandwich immunoassay of human immunoglobulin G with a LOD <10 ng/mL (S/N=3). The linear range was from 500 ng/mL to 100 microg/mL. This system has also been applied to the measurement of human immunoglobulin G from 15 real samples, demonstrating the potential of this platform for biomedical applications.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiaoou
authors.initials: X
authors.lastname: Su
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G