Effect of rhBMP-7 combined with two bone grafts on human periodontal ligament cell differentiation.
The purpose of this study was to evaluate the in vitro effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) combined with demineralised freeze-dried bone allograft (DFDBA) and an inorganic bovine material with a synthetic peptide (PepGen P-15) on human periodontal ligament (hPDL) cell differentiation, in a time-dependent manner. hPDL cells were cultured and treated with: (1) 500 ng/ml of rhBMP-7, (2) 10 mg of DFDBA or PepGen P-15 and (3) their combination. Cell differentiation was estimated after 48 and 72 h by measuring alkaline phosphatase (ALPase) activity and osteocalcin (OC) secretion. The presence of rhBMP-7, DFDBA, PepGen P-15, rhBMP-7 + DFDBA and rhBMP-7+ PepGen P-15 promoted a significant increase of ALPase activity after 48 and 72 h. The combination of rhBMP-7 with DFDBA or PepGen P-15 did not lead to significant OC secretion. The results of this study imply that rhBMP-7 stimulates the early osteoblastic differentiation of hPDL cells and that DFDBA and PepGen P-15 could serve as carriers for rhBMP-7.
Dereka
X E
XE
Department of Periodontology, School of Dentistry, University of Athens, Athens, Greece. dereka@otenet.gr
Markopoulou
C E
CE
Mamalis
A
A
Vrotsos
I A
IA
D000328:Adult; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000494:pharmacology*; D018786:Bone Substitutes / Q000627:therapeutic use; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology*; D016896:Treatment Outcome; D055815:Young Adult
D055419:Bone Morphogenetic Protein 7; D018786:Bone Substitutes; C403137:PepGen P-15; D011994:Recombinant Proteins