DNA binding proteins of the filamentous phages CTXphi and VGJphi of Vibrio cholerae.
The native product of open reading frame 112 (orf112) and a recombinant variant of the RstB protein, encoded by Vibrio cholerae pathogen-specific bacteriophages VGJphi and CTXphi, respectively, were purified to more than 90% homogeneity. Orf112 protein was shown to specifically bind single-stranded genomic DNA of VGJphi; however, RstB protein unexpectedly bound double-stranded DNA in addition to the single-stranded genomic DNA. The DNA binding properties of these proteins may explain their requirement for the rolling circle replication of the respective phages and RstB's requirement for single-stranded-DNA chromosomal integration of CTXphi phage dependent on XerCD recombinases.
Falero
Alina
A
Department of Molecular Biology, National Center for Scientific Research, P.O. Box 6414, Ave. 25 y 158, Cubanacán, Playa, Ciudad de la Habana, Cuba. alina.falero@cnic.edu.cu
Caballero
Andy
A
Ferrán
Beatriz
B
Izquierdo
Yovanny
Y
Fando
Rafael
R
Campos
Javier
J
D001287:Attachment Sites, Microbiological; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral; D017912:Inovirus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014734:Vibrio cholerae / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D016662:Virus Integration
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D004247:DNA