title: Gene expression profiling of monocytes displaying herpes simplex virus 1 induced dysregulation of antifungal defences.
abstract: Recently, we showed that herpes simplex virus 1 (HSV-1)-infected monocytes have altered antifungal defences, in particular they show augmented phagocytosis of Candida albicans followed by a failure of the intracellular killing of the ingested fungi. On the basis of these functional data, comparative studies were carried out on the gene expression profile of cells infected with HSV-1 and/or C. albicans in order to investigate the molecular mechanisms underlying such virus-induced dysfunction. Affymetrix GeneChip technology was used to evaluate the cell transcription pattern, focusing on genes involved in phagocytosis, fungal adhesion, antimicrobial activity and apoptosis. The results indicated there was: (a) prevalent inhibition of opsonin-mediated phagocytosis, (b) upregulation of several pathways of antibody- and complement-independent phagocytosis, (c) inhibition of macrophage activation, (d) marked dysregulation of oxidative burst, (e) induction of apoptosis.
authors.affiliation: Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Cermelli
authors.affiliation: Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Carlotta Francesca
authors.initials: CF
authors.lastname: Orsi
authors.affiliation: Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Cuoghi
authors.affiliation: Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ardizzoni
authors.affiliation: Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Tagliafico
authors.affiliation: Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Rachele
authors.initials: R
authors.lastname: Neglia
authors.affiliation: Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Samuele
authors.initials: S
authors.lastname: Peppoloni
authors.affiliation: Department of Public Health Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Blasi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002176:Candida albicans / Q000472:pathogenicity; D002448:Cell Adhesion / Q000235:genetics; D002460:Cell Line; D002522:Chlorocebus aethiops; D020869:Gene Expression Profiling; D018259:Herpesvirus 1, Human / Q000472:pathogenicity* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology / Q000502:physiology; D006801:Humans; D008262:Macrophage Activation / Q000235:genetics; D009000:Monocytes / Q000276:immunology / Q000473:pathology / Q000502:physiology* / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D009895:Opsonin Proteins / Q000502:physiology; D010587:Phagocytosis / Q000235:genetics; D016897:Respiratory Burst / Q000235:genetics; D014709:Vero Cells
chemical_list: D009895:Opsonin Proteins