title: Effect of strain and inoculation dose of classical swine fever virus on within-pen transmission.
abstract: To improve the understanding of the dynamics and options for control of classical swine fever (CSF), more quantitative knowledge is needed on virus transmission. In this study, virus excretion and within-pen transmission of a strain of low, moderate and high virulence were quantified. Furthermore, the effect of inoculation dose on excretion and transmission were studied. The transmission was quantified using a stochastic susceptible-exposed-infectious-recovered (SEIR) model. Five transmission trials were conducted with ten pigs each. In each trial, three pigs were inoculated with the low virulent strain Zoelen, a low (10(2) TCID(50)), middle (10(3.5) TCID(50)), or high dose (10(5) TCID(50)) of the moderately virulent strain Paderborn, or the highly virulent strain Brescia. The other seven pigs in each trial served as contact pigs. None of the pigs inoculated with the low dose of the Paderborn strain were infected. When it was assumed that the infectiousness of the pigs coincided with virus isolation positive oropharyngeal fluid and/or faeces, no significant differences in transmission rate beta and basic reproduction ratio R(0) between the high inoculation dose of the Paderborn strain (beta= 1.62/day, R(0) = 35.9) and the Brescia strain (beta= 2.07/day, R(0)= 17.5) were observed. When the middle dose of the Paderborn strain was used for inoculation, the beta (5.38/day) was not significantly higher than the Brescia strain or the high inoculation dose of the Paderborn strain, but the R(0) (148) was significantly higher. Infection with the Zoelen strain resulted in a significantly lower beta and R(0) (beta= 0/day, R(0) = 0) than the other strains.
authors.affiliation: Department of Virology, Central Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB, Lelystad, The Netherlands. eefke.weesendorp@wur.nl
authors.forename: Eefke
authors.initials: E
authors.lastname: Weesendorp
authors.forename: Jantien
authors.initials: J
authors.lastname: Backer
authors.forename: Arjan
authors.initials: A
authors.lastname: Stegeman
authors.forename: Willie
authors.initials: W
authors.lastname: Loeffen
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000635:transmission*; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000472:pathogenicity*; D005243:Feces / Q000821:virology; D008297:Male; D012463:Saliva / Q000821:virology; D013552:Swine; D014766:Viremia; D017201:Virus Shedding