[Synthesis and properties of a new conformational antigen modeling an extracellular region of beta(1)-adrenoreceptor].
Two fragments corresponding to the 125-133 and 206-218 sequences of a molecule of the beta(1) adrenoreceptor (autoantibodies to this protein are often found in patients with dilated cardiomyopathy) were synthesized by the solid phase method with the use of Fmoc technology. Two new conformational antigens were prepared by directed (regioselective) and undirected (spontaneous) formation of intramolecular and intermolecular disulfide bridges between the corresponding cysteine residues of the synthesized peptides. One of these antigens consisted of a mixture of disulfide isomers, and another antigen was an isomer with a natural arrangement of S-S bridges. Immunosorbents were obtained by immobilization of the synthesizes antigens on the bromocyanogenactivated sepharose and applied to the removal of autoantibodies in a beta(1)-adrenoreceptor from the blood plasma of patients. We demonstrated that the sorbents on the basis of the conformational antigens were more effective in comparison with those containing linear peptide precursors.
Sidorova
M V
MV
Russian Cardiological Research and Production Complex, Russian Ministry of Public Health, ul. 3-ya Cherepkovskaya 15a, 121552 Russia. peptide@cardio.ru
Pal'keeva
M E
ME
Molokoedov
A S
AS
Az'muko
A A
AA
Sekridova
A V
AV
Ovchinnikov
M V
MV
Levashov
P A
PA
Afanas'eva
O I
OI
Berestetskaia
Yu V
YV
Afanas'eva
M I
MI
Razova
O A
OA
Bespalova
Zh D
ZhD
Pokrovskiĭ
S N
SN
D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000302:isolation & purification; D002311:Cardiomyopathy, Dilated / Q000097:blood / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007163:Immunosorbent Techniques; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D017434:Protein Structure, Tertiary; D018342:Receptors, Adrenergic, beta-1 / Q000737:chemistry* / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies; D004220:Disulfides; D010455:Peptides; D018342:Receptors, Adrenergic, beta-1