title: Prevalence of VP7 and VP4 genotypes of human group A rotavirus in infants and children with acute diarrhea in a northern city of Bangladesh.
abstract: The objective of this cross sectional prospective study was to determine rotavirus etiology and prevalence of the different rotavirus genotypes causing acute gastroenteritis in infants and children (</=5years) in Mymensingh Medical College Hospital, Bangladesh. The study was conducted during the period from January 2005 to June 2006. A total of 962 stool specimens from diarrheic children were analyzed by Polyacrylamide Gel Electrophoresis (PAGE). Rotavirus detection rate was 31.3% (301/962). Among the 71 representative rotavirus specimens examined genetically by RT-PCR, the most frequent G genotypes were G2 (57.7%), followed by G1 (21.1%), and G9 (15.5%). The G12 was detected from two specimens (2.8%) and was the first report in the study area. The predominant P genotypes were P[4] (54.9%), followed by P[6] (19.7%), and P[8] (15.5%). Among the single infection, the predominant G/P combinations were G2P[4] (52.1%), followed by G1P[8] (10%), and G1P[6] (8.5%). Nucleotide Sequence identity of VP7 gene of G2 rotaviruses were higher than 99.0% with each other and these G2 viruses showed genetically lower identity to G2 rotaviruses reported elsewhere in the world, except for some strains reported in African countries. All the G2 strains sequenced in this study were grouped into single cluster in phylogenetic tree for G2 strains. As rotavirus vaccine is implemented in immunization program worldwide, the hospital based surveillance study will provide valuable information to assess the future impact of vaccination.
authors.affiliation: Department of Microbiology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh. drshyamal10@yahoo.com
authors.forename: S K
authors.initials: SK
authors.lastname: Paul
authors.forename: M A
authors.initials: MA
authors.lastname: Hossain
authors.forename: M U
authors.initials: MU
authors.lastname: Ahmed
authors.forename: M M
authors.initials: MM
authors.lastname: Alam
authors.forename: A K
authors.initials: AK
authors.lastname: Musa
authors.forename: A K
authors.initials: AK
authors.lastname: Shamsuzzaman
authors.forename: M N
authors.initials: MN
authors.lastname: Islam
authors.forename: S K
authors.initials: SK
authors.lastname: Saha
mesh_terms: D000208:Acute Disease; D000956:Antigens, Viral / Q000235:genetics*; D001459:Bangladesh / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D015995:Prevalence; D011446:Prospective Studies; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics; D016036:Seroepidemiologic Studies
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus