title: Validation of an HPLC method for determination of pentoxifylline in human plasma and its application to pharmacokinetic study.
abstract: An HPLC method suitable for routine determination of pentoxifylline in human plasma has been adapted and validated. Sample preparation was done by solid-phase extraction. Chloramphenicol was used as the internal standard. The linear range was from 15-400 ng/mL (r2 = 0.9994), with a limit of quantitation of 15 ng/mL. The limit of detection was found to be 5 ng/mL. The intra- and interday accuracy ranged from 98.0 to 110.2% and the coefficient of variation was not more than 8.8% for both intra- and interday precision. The absolute recoveries of pentoxifylline and chloramphenicol from human plasma were >97%. The method was validated with excellent specificity, accuracy, precision, recovery, and stability. The pharmacokinetic study of a generic pentoxifylline 400 mg tablet in healthy Thai male volunteers after a single dose administration was determined by this developed assay.
authors.affiliation: Naresuan University, Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry and Pharmacognosy, Phitsanulok 65000, Thailand. pattana9@excite.com
authors.forename: Pattana
authors.initials: P
authors.lastname: Sripalakit
authors.forename: Aurasorn
authors.initials: A
authors.lastname: Saraphanchotiwitthaya
mesh_terms: D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D008297:Male; D010431:Pentoxifylline / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D010726:Phosphodiesterase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D010726:Phosphodiesterase Inhibitors; D010431:Pentoxifylline