NMR assignment of the nonstructural protein nsp3(1066-1181) from SARS-CoV.
Sequence-specific NMR assignments of the globular core comprising the residues 1066-1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [(13)C, (15)N]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the (13)C chemical shifts.
Serrano
Pedro
P
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., MB-44, La Jolla, CA 92037, USA.
Johnson
Margaret A
MA
Chatterjee
Amarnath
A
Pedrini
Bill
B
Wüthrich
Kurt
K
D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009587:Nitrogen Isotopes / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011522:Protons; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D017361:Viral Nonstructural Proteins; C510959:Nsp3 protein, SARS-CoV; D012324:RNA-Dependent RNA Polymerase