Marinimicrobium locisalis sp. nov., isolated from a marine solar saltern, and emended description of the genus Marinimicrobium.
A Gram-negative, motile and rod-shaped bacterial strain, designated ISL-43T, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-43T grew optimally at pH 7.0-8.0 and 30 degrees C and in the presence of approximately 2% NaCl. It contained Q-8 as the predominant ubiquinone and C16:0, C19:0omega8c, C16:1omega7c and/or iso-C15:0 2-OH, and C18:1omega7c as the major fatty acids. The DNA G+C content was 58.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-43T fell within the genus Marinimicrobium, clustering with Marinimicrobium agarilyticum M18T with a bootstrap value of 100%. Strain ISL-43T exhibited 16S rRNA gene sequence similarity values of 98.1 and 95.5% to M. agarilyticum M18T and Marinimicrobium koreense M9T, respectively. Strain ISL-43T exhibited DNA-DNA relatedness values of 17 and 10% to M. agarilyticum KCTC 12357T and M. koreense KCTC 12356T, respectively. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-43T represents a novel species within the genus Marinimicrobium, for which the name Marinimicrobium locisalis sp. nov. is proposed. The type strain is ISL-43T (=KCTC 22484T=CCUG 56757T).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribb.re.kr
Kang
So-Jung
SJ
Jung
Yong-Taek
YT
Oh
Tae-Kwang
TK
D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride