NMR resonance assignment of DnaE intein from Nostoc punctiforme.
DnaE intein from Nostoc punctiforme (Npu) is one of naturally occurring split inteins, which has robust protein splicing activity. Highly efficient trans-splicing activity of NpuDnaE intein could widen various biotechnological applications. However, structural basis of the efficient protein splicing activity is poorly understood. As a first step toward better understanding of protein trans-splicing mechanism, we present the backbone and side-chain resonance assignments of a single chain variant NpuDnaE intein as determined by triple resonance experiments with [(13)C,(15)N]-labeled protein.
Heinämäki
Kimmo
K
Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, Helsinki, 00014, Finland.
Oeemig
Jesper S
JS
Pääkkönen
Kimmo
K
Djupsjöbacka
Janica
J
Iwaï
Hideo
H
D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D004258:DNA Polymerase III / Q000737:chemistry*; D047668:Inteins; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000737:chemistry; D046937:Nostoc / Q000737:chemistry*; D011522:Protons
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III