[Analysis of flavonoids in Rhododendron mariae by UPLC/ Q-TOF-MS].
OBJECTIVE
To investigate the flavonoids in Rhododendron mariae.


METHODS
The constituents in R. mariae were determined by UPLC/Q-TOF-MS. The chromatographic separation was performed on a C18 column (2.1 mm x 50 mm, 1.7 microm) with a gradient elufion of methanol-water containing 0.1% formic acid. The mass specfrometer eqaipped with elecfrospay ionizafion source was usedas defecfor and operated in data was collected under the positive and negative ion modes.


RESULTS
Seven constituents were identified as myricetin-3-O-beta-D-gluconside (1), myricetin-3'-O-beta-D-xylopyraoside (2), hyperoside (3), isoquercitrin (4), avicularin (5), quercitroside (6) and quercetin (7).


CONCLUSIONS
In this study, the main flavonoids in R. mariae were separated by UPLC, and identified through the information of positive ion and negative ion and relative molecular mass which were determined by Q-TOF-MS. It is an accurate and effective method which can be applied for the constituent identification of R. mariae.
Huang
Huiqiang
H
Central Laboratory of Guangdong Pharmaceutical University, Guangzhou 510006, China. hhqwxh@163.com
Feng
Yifan
Y
Rui
Wen
W
Jiang
Miaomiao
M
Han
Liang
L
D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D029793:Rhododendron / Q000737:chemistry*
D005419:Flavonoids