title: Replisome progression complex links DNA replication to sister chromatid cohesion in Xenopus egg extracts.
abstract: Cohesin-mediated sister chromatid cohesion is established during the S-phase, and recent studies demonstrate that a cohesin protein ring concatenates sister DNA molecules. However, little is known about how DNA replication is linked to the establishment of sister chromatid cohesion. Here, we used Xenopus egg extracts to show that AND-1 and Tim1-Tipin, homologues of Saccharomyces cerevisiae Ctf4 and Tof1-Csm3, respectively, are associated with the replisome and are required for proper establishment of the cohesion observed in the M-phase extracts. Immunodepletion of both AND-1 and Tim1-Tipin from the extracts leads to aberrant sister chromatid cohesion, which is similarly induced by the depletion of cohesin. These results demonstrate that AND-1 and Tim1-Tipin are key factors linking DNA replication and establishment of sister chromatid cohesion. On the basis of the physical interactions between AND-1 and DNA polymerases, we discuss a model to describe how replisome progression complex establishes sister chromatid cohesion.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kubota
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tsujimura
authors.forename: Maya
authors.initials: M
authors.lastname: Kumano
authors.forename: Hisao
authors.initials: H
authors.lastname: Masai
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Takisawa
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins; D002455:Cell Division; D002842:Chromatids / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase; D010063:Ovum / Q000378:metabolism*; D014020:Tissue Extracts / Q000378:metabolism*; D014981:Xenopus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D000097722:Cohesins
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D004259:DNA-Directed DNA Polymerase; D014020:Tissue Extracts; D029867:Xenopus Proteins; D000097722:Cohesins; C524403:Tim1 protein, Xenopus; C106931:WDHD1 protein, Xenopus; C524402:tipin protein, Xenopus