title: [MTHFR genetic variability on coronary artery disease development].
abstract: OBJECTIVE
  Increased homocysteine (Hcy) concentration is considered a risk factor for coronary artery disease (CAD). Genetic alterations of the metylenetetrahydrofolate reductase (MTHFR) enzyme could reduce its thermolability and alter the Hcy metabolism, contributing to development of atherosclerotic lesions. Objective of this study was to investigate the relation between MTHFR C677T and A1298C polymorphisms and presence, extension, and severity of CAD.
  
  
  METHODS
  One hundred seventy-five patients with CAD confirmed by angiography, and 108 individuals without CAD (control group) were evaluated. MTHFR C677T polymorphism was investigated by polymerase chain reaction (PCR) followed by enzyme digestion. The genotyping of the MTHFR A1298C polymorphism was performed by PCR allele-specific method.
  
  
  RESULTS
  Frequency of the altered allele MTHFR 677C was 0.38 in the CAD group and 0.37 in the control group. Regarding the polymorphic allele MTHFR 1298C, frequency was 0.22 and 0.27, respectively. The genotype distribution MTHFR C677T and A1298C did not differ regarding number of affected vessels (P > 0.05). Also, relation between MTHFR C677T polymorphism and degree of arterial obstruction was not observed (P > 0.05), as well as the MTHFR A1298C polymorphism (P > 0.05).
  
  
  CONCLUSIONS
  Results did not show association between MTHFR A1298C and MTHFR C677T polymorphisms and presence, extension or severity of CAD.
authors.affiliation: Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP.
authors.forename: Patrícia Matos
authors.initials: PM
authors.lastname: Biselli
authors.forename: Alexandre Rodrigues
authors.initials: AR
authors.lastname: Guerzoni
authors.forename: Eny Maria
authors.initials: EM
authors.lastname: Goloni-Bertollo
authors.forename: Moacir Fernandes de
authors.initials: MF
authors.lastname: Godoy
authors.forename: Juliana Aparecida Barcelos
authors.initials: JA
authors.lastname: Abou-Chahla
authors.forename: Erika Cristina
authors.initials: EC
authors.lastname: Pavarino-Bertelli
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)