Erroneous reporting of vancomycin susceptibility for Staphylococcus spp. by Vitek software version 2.01.
The reporting of vancomycin resistance in Staphylococcus spp. has enormous therapeutic and epidemiological consequences. We evaluated Vitek software version 2.01 by the CLSI-recommended broth dilution method as well as the CDC-recommended vancomycin screen agar and Etest for vancomycin susceptibility testing of Staphylococcus spp. Of the 105 isolates of Staphylococci tested by the above methods, Vitek 2.01 gave 16 (15%) a false vancomycin intermediate/resistant phenotype. Laboratories using automated systems for routine microbiological susceptibility testing must confirm such resistance by validated methods.
Behera
Bijayini
B
Microbiology Division, Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, India.
Mathur
Purva
P
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003951:Diagnostic Errors; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000187:drug effects* / Q000302:isolation & purification; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D014640:Vancomycin