Microvalves actuated sandwich immunoassay on an integrated microfluidic system.
In this study, we developed an integrated and automatic microfluidic immunoassay system, which comprised a multi-layer immunoassay microfluidic chip and a rotary confocal LIF scanner. The normally closed elastomeric microvalves were integrated on the multi-layer microfluidic chip to realize flexible reagents delivery for the rapid immunoassay. The various operations of immunoassay including coating, blocking and washing could be performed on a single chip with reduced reagents consumption and analysis time. The homemade rotary LIF scanner was able to detect the signals from the multichannels sequentially in a short period of time, enabling the potential capability for high-throughput immunoassay. The performance of this system was demonstrated by the sandwich immunoassay of human immunoglobulin G with a LOD <10 ng/mL (S/N=3). The linear range was from 500 ng/mL to 100 microg/mL. This system has also been applied to the measurement of human immunoglobulin G from 15 real samples, demonstrating the potential of this platform for biomedical applications.
Gao
Xinghua
X
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Jiang
Lei
L
Su
Xiaoou
X
Qin
Jianhua
J
Lin
Bingcheng
B
D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G