Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size.
The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression was associated to apical meristem restructuration, protoplasts with higher (3)H-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration of the CDC27a subunit.
Rojas
Cristian Antonio
CA
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.
Eloy
Nubia Barbosa
NB
Lima
Marcelo de Freitas
Mde F
Rodrigues
Roberta Lopes
RL
Franco
Luciana Ozório
LO
Himanen
Kristiina
K
Beemster
Gerrit T S
GT
Hemerly
Adriana Silva
AS
Ferreira
Paulo Cavalcanti Gomes
PC
D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D048429:Cell Size; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D047468:Immunoprecipitation; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013936:Thymidine / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D014316:Tritium / Q000378:metabolism; D054875:Ubiquitination
D029681:Arabidopsis Proteins; C543806:CDC27a protein, Arabidopsis; D018797:Cell Cycle Proteins; D014316:Tritium; D013936:Thymidine