title: The use of injectable spherically symmetric cell aggregates self-assembled in a thermo-responsive hydrogel for enhanced cell transplantation.
abstract: Typical cell transplantation techniques involve the administration of dissociated cells directly injected into muscular tissues; however, retention of the transplanted cells at the sites of the cell graft is frequently limited. An approach, using spherically symmetric aggregates of cells with a relatively uniform size self-assembled in a thermo-responsive methylcellulose hydrogel system, is reported in the study. The obtained cell aggregates preserved their endogenous extracellular matrices (ECM) and intercellular junctions because no proteolytic enzyme was used when harvesting the cell aggregates. Most of the cells within aggregates (with a radius of approximately 100 microm) were viable as indicated by the live/dead staining assay. After injection through a needle, the cell aggregates remained intact and the cells retained their activity upon transferring to another growth surface. The cell aggregates obtained under sterile conditions were transplanted into the skeletal muscle of rats via local injection. The dissociated cells were used as a control. It was found that the cell aggregates can provide an adequate physical size to entrap into the muscular interstices and offer a favorable ECM environment to enhance retention of the transplanted cells at the sites of the cell graft. These results indicated that the spherically symmetric cell aggregates developed in the study may serve as a cell delivery vehicle for therapeutic applications.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, ROC.
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Lee
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Chang
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Yeh
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Chen
authors.forename: Kurt M
authors.initials: KM
authors.lastname: Lin
authors.forename: Chieh-Cheng
authors.initials: CC
authors.lastname: Huang
authors.forename: Yen
authors.initials: Y
authors.lastname: Chang
authors.forename: Hsing-Wen
authors.initials: HW
authors.lastname: Sung
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D008422:Materials Testing; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000601:surgery*; D051381:Rats; D011917:Rats, Inbred Lew; D013102:Spherocytes; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels