Self-assembly formation of multiple DNA-tethered lipid bilayers.
Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture.
Tabaei
Seyed R
SR
Dept. of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden.
Jönsson
Peter
P
Brändén
Magnus
M
Höök
Fredrik
F
D004247:DNA / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008962:Models, Theoretical
D008051:Lipid Bilayers; D004247:DNA; D004134:Dimyristoylphosphatidylcholine