Collagen and glycopolymer based hydrogel for potential corneal application.
6-Methacryloyl-alpha-D-galactopyranose (MG) was synthesized, and characterized by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectrometry, and single-crystal X-ray diffraction. A series of interpenetrating polymer network (IPN) hydrogels was fabricated by simultaneously photocuring MG crosslinked by poly(ethylene glycol) diacrylate and chemically crosslinking type I collagen with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The successful incorporation of the glycopolymer, polymer MG, into collagen hydrogel was confirmed by FTIR and solid-state (13)C NMR. The optical characteristics of the IPN hydrogels are comparable to those of human corneas. The tensile strength and modulus of the hydrogels are enhanced by incorporation of polymer MG in comparison to that of the control collagen hydrogel. Biodegradation results indicated that polymer MG enhanced the stability of the composite hydrogels against collagenase. In vitro results demonstrated that the IPN hydrogel supported the adhesion and proliferation of human corneal epithelial cells and outperformed human cornea in blocking bacteria adhesion. Taken together, the IPN hydrogel might be a promising material for use in corneal lamellar keratoplasty.
Deng
Chao
C
University of Ottawa Eye Institute, Ottawa, Ont., Canada K1H 8L6.
Li
Fengfu
F
Hackett
Joanne M
JM
Chaudhry
Shazia H
SH
Toll
Floyd N
FN
Toye
Baldwin
B
Hodge
William
W
Griffith
May
M
D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry*; D001673:Biodegradation, Environmental; D003094:Collagen / Q000737:chemistry*; D024042:Collagen Type I / Q000378:metabolism; D003315:Cornea / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D016039:Corneal Transplantation / Q000379:methods; D020100:Hydrogels / Q000737:chemistry*; D007634:Keratitis / Q000188:drug therapy*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013211:Staphylococcus aureus / Q000378:metabolism; D013388:Succinimides / Q000737:chemistry; D013552:Swine; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D024042:Collagen Type I; D020100:Hydrogels; D011108:Polymers; D013388:Succinimides; D003094:Collagen; C001426:N-hydroxysuccinimide