Investigating unsaturated fat, monensin, or bromoethanesulfonate in continuous cultures retaining ruminal protozoa. I. Fermentation, biohydrogenation, and microbial protein synthesis.
Methane is an end product of ruminal fermentation that is energetically wasteful and contributes to global climate change. Bromoethanesulfonate, animal-vegetable fat, and monensin were compared with a control treatment to suppress different functional groups of ruminal prokaryotes in the presence or absence of protozoa to evaluate changes in fermentation, digestibility, and microbial N outflow. Four dual-flow continuous culture fermenter systems were used in 4 periods in a 4 x 4 Latin square design split into 2 subperiods. In subperiod 1, a multistage filter system (50-microm smallest pore size) retained most protozoa. At the start of subperiod 2, conventional filters (300-microm pore size) were substituted to efflux protozoa via filtrate pumps over 3 d; after a further 7 d of adaptation, the fermenters were sampled for 3 d. Treatments were retained during both subperiods. Flow of total N and digestibilities of NDF and OM were 18, 16, and 9% higher, respectively, for the defaunated subperiod but were not different among treatments. Ammonia concentration was 33% higher in the faunated fermenters but was not affected by treatment. Defaunation increased the flow of nonammonia N and bacterial N from the fermenters. Protozoal counts were not different among treatments, but bromoethanesulfonate increased the generation time from 43.2 to 55.6 h. Methanogenesis was unaffected by defaunation but tended to be increased by unsaturated fat. Defaunation did not affect total volatile fatty acid production but decreased the acetate:propionate ratio; monensin increased production of isovalerate and valerate. Biohydrogenation of unsaturated fatty acids was impaired in the defaunated fermenters because effluent flows of oleic, linoleic, and linolenic acids were 60, 77, and 69% higher, and the ratio of vaccenic acid:unsaturated FA ratio was decreased by 34% in the effluent. This ratio was increased in both subperiods with the added fat diet, indicating an accumulation of intermediates of biohydrogenation. However, the flow of 18:2 conjugated linoleic acid was unaffected by defaunation or by treatments other than added fat. The flows of trans-10, trans-11, and total trans-18:1 fatty acids were not affected by monensin or faunation status.
Karnati
S K R
SK
Ohio State University Interdisciplinary Nutrition Program (OSUN), Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA. karnati.1@osu.edu
Sylvester
J T
JT
Ribeiro
C V D M
CV
Gilligan
L E
LE
Firkins
J L
JL
D000476:Alkanesulfonates / Q000494:pharmacology*; D000641:Ammonia / Q000032:analysis; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D046508:Culture Techniques; D004042:Dietary Fats, Unsaturated / Q000378:metabolism*; D056890:Eukaryota / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D005232:Fatty Acids, Volatile / Q000032:analysis; D005260:Female; D005285:Fermentation / Q000187:drug effects*; D005766:Gastrointestinal Contents / Q000737:chemistry; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D006865:Hydrogenation / Q000187:drug effects; D008697:Methane / Q000378:metabolism; D008985:Monensin / Q000494:pharmacology*; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012417:Rumen / Q000378:metabolism / Q000469:parasitology
D000476:Alkanesulfonates; D000890:Anti-Infective Agents; D000981:Antiprotozoal Agents; D004042:Dietary Fats, Unsaturated; D005232:Fatty Acids, Volatile; D006842:Hydrocarbons, Brominated; D000641:Ammonia; D008985:Monensin; D009584:Nitrogen; D008697:Methane