Simultaneous determination of eleven quinolones in animal feed by liquid chromatography with fluorescence and ultraviolet absorbance detection.
A rapid and simple multi-residue procedure is described for assaying eleven quinolones (cinoxacin, ciprofloxacin, danofloxacin, difloxacin, enrofloxacin, flumequine, marbofloxacin, nalidixic acid, norfloxacin, oxolinic acid and sarafloxacin) in feeds at sub-additive levels (1-5 mg kg(-1)). Five grams of sample were extracted by a metaphosphoric acid/acetonitrile mixture (70/30, v/v) and purified onto OASIS HLB cartridges. The determination was achieved by liquid chromatography (LC) using a GEMINI C18 analytical column both with fluorescence detection (FD) and photodiode-array (DAD). Limits of detection for each drug were in the range 0.04-0.8 mg kg(-1). Above the limit of quantification (LOQ), in poultry feed the recoveries were from 69 to 98% with relative standard deviations less than or equal 10%. Finally the measurement uncertainty was estimated using the bottom-up approach.
Galarini
Roberta
R
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, I-06126 Perugia, Italy. r.galarini@izsum.it
Fioroni
Laura
L
Angelucci
Federico
F
Tovo
Gloria R
GR
Cristofani
Elisa
E
D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D015363:Quinolones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D015363:Quinolones