[Adaptive changes of ultrastructures of dentritic cells during maturation induced by lipopolysaccharide].
OBJECTIVE
To investigate the adaptive changes of ultrastructure of the dentritic cells (DC) before and after maturation.


METHODS
The murine bone marrow mononuclear cells were induced into immatured dendritic cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). The cell morphology was observed under an inverted phase contrast microscope, and the surface markers were detected by flow cytometry. Then the DC was induced to be matured with lipopolysaccharide (LPS) for 48 hours, the ultrastructures of DC was observed before and after maturation under transmission electron microscope and then a comparative analysis was doned.


RESULTS
The surface processes of matured dentritic cells stimulated by LPS decreased significantly, whereas the organelles and the diameter of nucleolus increased.


CONCLUSIONS
The distribution of surface processes may be associated with the antigen-presenting capacity of DC, and it is also a potential ruler of cell function and status.
Luo
De-Ping
DP
Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Chongqing Medical University, Chongqing 400015, China.
Li
Yong
Y
Li
Dai-Qing
DQ
Ji
Ping
P
Wang
Tao
T
Qiu
Li-Hua
LH
Liu
Ping
P
D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015847:Interleukin-4; D008070:Lipopolysaccharides; D051379:Mice
D008070:Lipopolysaccharides; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor