Label-free DNA sensor based on fluorescent cationic polythiophene for the sensitive detection of hepatitis B virus oligonucleotides.
Water-soluble fluorescent conjugated polymers can be used as an optical platform in highly sensitive DNA sensors. Here we report a simple label-free DNA sensor using poly(3-alkoxy-4-methylthiophene) to recognize and detect different oligonucleotide targets related to the YMDD gene mutation of hepatitis B virus. The concentration of surfactant Triton X-100, NaCl, the oligonucleotide capture probe and the oligonucleotide hybridization conditions have a great impact on fluorescence intensity. Under the optimum conditions, two types of oligonucleotide targets involving YMDD gene mutation of hepatitis B virus were successfully recognized. Moreover, there was a linear relationship between fluorescence intensity and the concentration of oligonucleotide target. The detection limit of the wild-type hepatitis B virus target is 88 pmol L(-1).
Guan
Hongliang
H
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Cai
Min
M
Chen
Lu
L
Wang
Yun
Y
He
Zhike
Z
D002412:Cations / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005453:Fluorescence; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification*; D009154:Mutation; D015345:Oligonucleotide Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis* / Q000235:genetics
D002412:Cations; D015342:DNA Probes; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D009841:Oligonucleotides