Corynebacterium maris sp. nov., a marine bacterium isolated from the mucus of the coral Fungia granulosa.
A bacterial strain, designated Coryn-1(T), was isolated from mucus of the coral Fungia granulosa (northern Red Sea, Gulf of Eilat, Israel) by growth and enrichment of micro-organisms in agar spheres and subsequent plating. The bacterium was found to be a Gram-positive, non-motile, halotolerant, heterotrophic coccobacillus. Comparative 16S rRNA gene sequence analyses showed that strain Coryn-1(T) belonged to the genus Corynebacterium, exhibiting the highest levels of similarity (94 %) with the 16S rRNA gene sequence of Corynebacterium halotolerans YIM 70093(T). The novel strain grew well at 0.5-4.0 % salinity, at pH 7.2-9.0 and at 30-37 degrees C. The major cellular fatty acids were oleic acid (C(18 : 1)omega9c; 58 %), palmitic acid (C(16 : 0); 30 %) and tuberculostearic acid (10-methyl-C(18 : 0); 12 %). The DNA G+C content was 66.6 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Coryn-1(T) represents a novel species, for which the name Corynebacterium maris sp. nov. is proposed. The type strain is Coryn-1(T) (=DSM 45190(T)=LMG 24561(T)).
Ben-Dov
Eitan
E
Department of Biotechnology Engineering, and National Institute for Biotechnology, Ben-Gurion University of the Negev, PO Box 653, Be'er-Sheva 84105, Israel.
Ben Yosef
Dafna Zeevi
DZ
Pavlov
Valentina
V
Kushmaro
Ariel
A
D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D003352:Corynebacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S