title: Highly sensitive and selective colorimetric and off-on fluorescent chemosensor for Cu2+ in aqueous solution and living cells.
abstract: The design and synthesis of a novel rhodamine spirolactam derivative and its application in fluorescent detections of Cu(2+) in aqueous solution and living cells are reported. The signal change of the chemosensor is based on a specific metal ion induced reversible ring-opening mechanism of the rhodamine spirolactam. It exhibits a highly sensitive "turn-on" fluorescent response toward Cu(2+) in aqueous solution with an 80-fold fluorescence intensity enhancement under 10 equiv of Cu(2+) added. This indicates that the synthesized chemosensor effectively avoided the fluorescence quenching for the paramagnetic nature of Cu(2+) via its strong binding capability toward Cu(2+). With the experimental conditions optimized, the probe exhibits a dynamic response range for Cu(2+) from 8.0 x 10(-7) to 1.0 x 10(-5) M, with a detection limit of 3.0 x 10(-7) M. The response of the chemosensor for Cu(2+) is instantaneous and reversible. Most importantly, both the color and fluorescence changes of the chemosensor are remarkably specific for Cu(2+) in the presence of other heavy and transition metal ions (even those that exist in high concentration), which meet the selective requirements for biomedical and environmental monitoring application. The proposed chemosensor has been used for direct measurement of Cu(2+) content in river water samples and imaging of Cu(2+) in living cells with satisfying results, which further demonstrates its value of practical applications in environmental and biological systems.
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Zhang
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Han
authors.forename: Li
authors.initials: L
authors.lastname: Qiao
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Li
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Jian
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Shen
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D003124:Colorimetry / Q000379:methods*; D003300:Copper / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D012996:Solutions; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012996:Solutions; D014867:Water; D003300:Copper