Pyridoxal 5'-phosphate binding in lysine-modified PAMAM dendrimers: a biomimetic approach.
(G:3-7)-dendri-PAMAM-(APO-Phe-Lys)(x) (2, APO = aminopropanol, Phe = phenylalanine, Lys = lysine) were prepared and used in a binding study with pyridoxal 5'-phosphate. The results revealed a positive dendritic effect, and binding ability was found to vary with the environment. (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)(x) (2) demonstrated better binding ability at higher pH, and protonation of lysine was considered to affect binding. The strongest binding affinity was K(b) = 254.3 mM(-1) at pH 9, which was shown by (G:7)-dendri-PAMAM-(APO-Phe-Lys)(490) (2e).
Hsien
Kuang-Chan
KC
Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Chen
Hui-Ting
HT
Chen
Yi-Chen
YC
Chen
Yeh-Long
YL
Lu
Chi-Yu
CY
Kao
Chai-Lin
CL
D001665:Binding Sites; D050091:Dendrimers; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000737:chemistry*; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D011073:Polyamines / Q000138:chemical synthesis* / Q000737:chemistry; D011732:Pyridoxal Phosphate / Q000737:chemistry* / Q000378:metabolism
D050091:Dendrimers; C104700:PAMAM Starburst; D010455:Peptides; D011073:Polyamines; D010649:Phenylalanine; D011732:Pyridoxal Phosphate; D008239:Lysine