Intervention of Acidophilus-casei dahi and wheat bran against molecular alteration in colon carcinogenesis.
An in vivo trial was conducted on seventy five rats allocated to three groups, first group was DMH control group, second group was Wheat bran-DMH group (WB-DMH) in which wheat bran was given along with DMH (1,2-dimethylhydrazine) injection, third group was Wheat bran-DMH-Ac Dahi group in which both wheat bran and Acidophilus-casei dahi (a probiotic microorganisms fermented dairy product) was given along with DMH injections. Animals received subcutaneous injections of DMH at a dose rate of 20 mg/kg body weight, once weekly for 15 weeks. The rats were dissected at 40th week of experiment and comet assay was done in colonic cells to assess the DNA damage. The c-myc and cox-2 expression was studied in rat tumour. A significant reduction in DNA damage (48.2%) was observed in WB-DMH-Ac Dahi group as compared to DMH control group (87.8%). The c-myc and cox-2 mRNA level was found highest in DMH control group as compared to WB-DMH and WB-DMH-Ac Dahi group. The results of present study show the enhanced protective potential of Acidophilus-casei and wheat bran against DMH induced molecular alteration in colonic cells during carcinogenesis.
Kumar
Arvind
A
Animal Biochemistry Division, National Dairy Research Institute, Haryana, India. arvind00000@gmail.com
Singh
Nikhlesh Kumar
NK
Sinha
Pushpalata Rabindra
PR
Kumar
Raj
R
D019813:1,2-Dimethylhydrazine; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000517:prevention & control*; D020552:Comet Assay; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004043:Dietary Fiber / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007778:Lactobacillus / Q000187:drug effects / Q000502:physiology*; D009153:Mutagens / Q000633:toxicity; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015014:Yogurt / Q000382:microbiology*
D004043:Dietary Fiber; D009153:Mutagens; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D019813:1,2-Dimethylhydrazine