Herbal formula SYJN protect PC12 cells from neurotoxicity induced by corticosterone.
OBJECTIVE
SYJN is a Chinese herbal formula, containing four herbs: Bupleurum chinense DC., Curcuma aromatica Salisb., Perilla frutescens (Linn.) Britt. and Acorus tatarinowii Schott. Previous studies on the formula in our laboratory revealed an antidepressant-like effect on animal models of behavioral despair. However,the mechanisms underlying such antidepressant-like effect are yet to be understood. The aim of this work was to verify the previously established antidepressant-like effects on cell level using corticosterone-induced neurotoxicity in rat pheochromocytoma (PC12) cells to see if SYJN possesses any neuroprotective properties.


METHODS
PC12 cells were treated with 200 microM corticosterone in the absence or the presence of various concentrations of SYJN for 48 h. Then, cell viability, apoptosis, intracellular Ca(2+) ([Ca(2+)]i) concentration and caspase-3 activity were determined.


RESULTS
Following the exposure of PC12 cells to 200 microM corticosterone for 48 h, there were reductions in cell survival rate but increases in lactate dehydrogenase (LDH) release. In parallel, corticosterone caused significant elevations in DNA fragmentation, [Ca(2+)]i concentration and caspase-3 activity. However, when the PC12 cells were incubated with SYJN at different concentrations (10, 50 and 100mg/L) in the presence of 200 microM corticosterone for 48 h, the above effects were evidently alleviated in a dose-dependent manner.


CONCLUSIONS
SYJN could generate a neuroprotective effect on corticosterone-induced neurotoxicity in PC12 cells, suggesting a possible action pathway of SYJN in vivo by decreasing the [Ca(2+)]i concentration and caspase-3 activity.
Huang
Zhen
Z
College of Pharmacy, Zhejiang Chinese Medicine University, Hangzhou 310053, Zhejiang, China. zhen626@yahoo.com.cn
Mao
Qing-Qiu
QQ
Zhong
Xiao-Ming
XM
Feng
Chun-Rong
CR
Pan
Ai-Juan
AJ
Li
Zhao-Yi
ZY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003345:Corticosterone / Q000633:toxicity*; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008516:Medicine, Chinese Traditional / Q000379:methods; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C545641:SYJN herbal formula; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3; D002118:Calcium; D003345:Corticosterone