title: Improved isolation of glucuronan from algae and the production of glucuronic acid oligosaccharides using a glucuronan lyase.
abstract: A new source for the production of bioactive glucuronic acid oligosaccharides (GlcUAOs) from the depolymerization of green seaweed Ulva lactuca glucuronan (Algal glucuronan) has been investigated. Algal glucuronan purification was optimized by the acidic precipitation method which allowed us to separate the polysaccharide mixture extracted from the cell wall of Ulvalactuca using hot water containing sodium oxalate. A series of the GlcUAOs were obtained by enzyme degradation of algal glucuronan with a glucuronan lyase (GL) isolated from Trichoderma strain. The putative bioactive GlcUAOs generated were then purified by size-exclusion chromatography in gram quantity and characterized by (1)H/(13)C NMR spectroscopy and ESI-Q/TOF-mass spectrometry.
authors.affiliation: LPMV, Université de Picardie Jules Verne, IUT-GB Amiens, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France. redouan_elboutachfaiti@hotmail.com
authors.forename: Elboutachfaiti
authors.initials: E
authors.lastname: Redouan
authors.forename: Delattre
authors.initials: D
authors.lastname: Cedric
authors.forename: Petit
authors.initials: P
authors.lastname: Emmanuel
authors.forename: El Gadda
authors.initials: el G
authors.lastname: Mohamed
authors.forename: Courtois
authors.initials: C
authors.lastname: Bernard
authors.forename: Michaud
authors.initials: M
authors.lastname: Philippe
authors.forename: El Modafar
authors.initials: el M
authors.lastname: Cherkaoui
authors.forename: Courtois
authors.initials: C
authors.lastname: Josiane
mesh_terms: D002240:Carbohydrate Sequence; D003365:Costs and Cost Analysis; D020723:Glucuronic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000378:metabolism*; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology; D044445:Ulva / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; C088556:mucoric acid; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase