Role of the cytoplasmic tail amino acid sequences of Newcastle disease virus hemagglutinin-neuraminidase protein in virion incorporation, cell fusion, and pathogenicity.
To determine the role of amino acid sequences of the hemagglutinin-neuraminidase (HN) cytoplasmic tail in Newcastle disease virus (NDV) replication and pathogenicity, we generated recombinant NDVs with a deletion or point mutation in the N-terminal cytoplasmic tail. The first 2-amino-acid deletion in the cytoplasmic tail did not affect the biological characteristics of NDV. However, a 4-amino-acid deletion and the substitution of alanine for serine at position 6 affected cell fusion, pathogenicity, and colocalization of the HN and M proteins of NDV, indicating that these residues of the HN cytoplasmic tail are critical for its specific incorporation into virions.
Kim
Shin-Hee
SH
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742, USA.
Yan
Yongqi
Y
Samal
Siba K
SK
D000818:Animals; D002459:Cell Fusion; D002642:Chick Embryo; D003593:Cytoplasm / Q000378:metabolism*; D000939:Epitopes; D017353:Gene Deletion; D006023:Glycoproteins / Q000737:chemistry; D006388:Hemagglutinins / Q000737:chemistry*; D006801:Humans; D009439:Neuraminidase / Q000737:chemistry*; D009522:Newcastle disease virus / Q000235:genetics*; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D014771:Virion
D000939:Epitopes; D006023:Glycoproteins; D006388:Hemagglutinins; D011994:Recombinant Proteins; D009439:Neuraminidase