title: G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.
abstract: We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, Los Angeles, University of California, Los Angeles, Los Angeles, California, USA.
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Evans
authors.forename: John M
authors.initials: JM
authors.lastname: Olson
authors.forename: Kathy T
authors.initials: KT
authors.lastname: Ngo
authors.forename: Eunha
authors.initials: E
authors.lastname: Kim
authors.forename: Noemi E
authors.initials: NE
authors.lastname: Lee
authors.forename: Edward
authors.initials: E
authors.lastname: Kuoy
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Patananan
authors.forename: Daniel
authors.initials: D
authors.lastname: Sitz
authors.forename: Phuongthao
authors.initials: P
authors.lastname: Tran
authors.forename: Minh-Tu
authors.initials: MT
authors.lastname: Do
authors.forename: Kevin
authors.initials: K
authors.lastname: Yackle
authors.forename: Albert
authors.initials: A
authors.lastname: Cespedes
authors.forename: Volker
authors.initials: V
authors.lastname: Hartenstein
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Call
authors.forename: Utpal
authors.initials: U
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002999:Clone Cells; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology / Q000235:genetics*; D005470:Fluorometry; D017930:Genes, Reporter; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D016366:Open Reading Frames; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase