Detection and characterization of Enterobacteriaceae producing metallo-beta-lactamases in a tertiary-care hospital in Spain.
Carbapenem-resistant or intermediate (MIC >or=1 mg/L) clinical isolates (n = 12) of three species of Enterobacteriaceae (Klebsiella pneumoniae, Klebsiella oxytoca and Escherichia coli) were characterized. The isolates harboured integrons containing the VIM-1 metallo-beta-lactamase gene together with other resistance gene cassettes. In particular, the CTX-M-2 gene was detected in four of the K. pneumoniae isolates. The patient population was mostly paediatric and characterized by severe underlying illnesses that involved long-term hospitalization, major surgery and/or immunosuppressive and broad-spectrum antibiotic therapy.
Cendejas
E
E
Servicio de Microbiología y Unidad de Investigación, Hospital Universitario La Paz, Paseo de la Castellana, Madrid, Spain.
Gómez-Gil
R
R
Gómez-Sánchez
P
P
Mingorance
J
J
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000096:biosynthesis*; D005798:Genes, Bacterial; D006761:Hospitals; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D032023:Integrons; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D013030:Spain; D001618:beta-Lactamases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D001618:beta-Lactamases