[Determination of fluoxastrobin and fluacrypyrim residues in fruits and beverages by ultra performance liquid chromatograph].
A method was developed for the determination of fluoxastrobin and fluacrypyrim residues in fruits and beverages by ultra performance liquid chromatography with photo-diode array (UPLC-PDA) detection. The sample was extracted with ethyl acetate-cyclohexane (1:1, v/v) by ultrasonic, cleaned-up by gel permeation chromatography (GPC), and then determined by UPLC-PDA. The quantification was performed by external standard. A BEH C18 colunm (50 mm x 2.1 mm, 1.7 microm) was used, and water-acetonitrile (3:7, v/v) was used as mobile phase at a flow rate of 0.3 mL/min. The column temperature was set at 40 degrees C, and ultraviolet absorption wavelength was set at 251 nm. The calibration curves were linear between the peak area and the concentration in the range of 0.05-2 mg/L for fluoxastrobin and fluacrypyrim, the correlation coefficients were greater than 0.999. The average recoveries spiked in fruit and beverage matrices at the three concentration levels of 0.01, 0.05, 0.1 mg/kg ranged from 82.60% to 101.11% with the relative standard deviations of 5.4%-15.3%. The limits of detection (LOD) were not greater than 6 microg/kg and the limits of quantification (LOQ) were not greater than 20 microg/kg in fruit and beverage matrices for fluoxastrobin and fluacrypyrim.
Luo
Lili
L
College of Food Science and Engineering, Gansu Agricultural Unversity, Lanzhou 730070, China.
Bo
Haibo
H
Bi
Yang
Y
Wu
Yonglong
Y
D000179:Acrylates / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D010091:Oximes / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis*; D000073739:Strobilurins
D000179:Acrylates; D010091:Oximes; D010573:Pesticide Residues; D011743:Pyrimidines; D000073739:Strobilurins; C551593:fluacrypyrim; C550006:fluoxastrobin