title: CHO cells adhering to nitrogen-rich plasma-polymerised ethylene exhibit high production of a specific recombinant protein.
abstract: In many industrial applications, inadequate cell attachment can be a limitation, especially when serum-free media are used. Nitrogen-rich plasma-polymerised ethylene (PPE:N) exhibits high concentrations of polar groups that can help to promote the attachment of weakly adherent cell types. Tissue plasminogen activator-producing Chinese hamster ovary (CHO) cells, adapted to suspension, were grown in the presence PPE:N flakes and were found to adhere to them. The growth rate was reduced, but cell viability was enhanced and their metabolism was more efficient, with generally higher recombinant protein productivity. Finally, cell adhesion on PPE:N surfaces was found to be independent of integrins, and was probably mediated by certain non-specific interactions with the PPE:N surface.
authors.affiliation: Department of Chemical Engineering, Ecole Polytechnique de Montréal, Montréal, QC, Canada.
authors.forename: Anne
authors.initials: A
authors.lastname: Gigout
authors.forename: Sophie
authors.initials: S
authors.lastname: Levasseur
authors.forename: Pierre-Luc
authors.initials: PL
authors.lastname: Girard-Lauriault
authors.forename: Michael D
authors.initials: MD
authors.lastname: Buschmann
authors.forename: Michael R
authors.initials: MR
authors.lastname: Wertheimer
authors.forename: Mario
authors.initials: M
authors.lastname: Jolicoeur
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D020099:Coated Materials, Biocompatible; D006224:Cricetinae; D003412:Cricetulus; D005030:Ethylenes / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008422:Materials Testing; D009584:Nitrogen / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013499:Surface Properties; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis
chemical_list: D020099:Coated Materials, Biocompatible; D005030:Ethylenes; D019012:Integrin beta1; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D005973:Glutamine; D019344:Lactic Acid; C036216:ethylene; D010959:Tissue Plasminogen Activator; D005947:Glucose; D009584:Nitrogen