title: [Preparation and characterization of monoclonal antibodies to the cholera toxin].
abstract: Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho-Maklaya 16/10, V-437, Moscow, 117997 Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Petrova
authors.forename: R L
authors.initials: RL
authors.lastname: Komaleva
authors.forename: O E
authors.initials: OE
authors.lastname: Lakhtina
authors.forename: L V
authors.initials: LV
authors.lastname: Samokhvalova
authors.forename: N A
authors.initials: NA
authors.lastname: Kalinina
authors.forename: N S
authors.initials: NS
authors.lastname: Shoshina
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Rubina
authors.forename: M A
authors.initials: MA
authors.lastname: Filippova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Vertiev
authors.forename: T I
authors.initials: TI
authors.lastname: Valyakina
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000276:immunology; D002772:Cholera Toxin / Q000276:immunology*; D003429:Cross Reactions; D029968:Escherichia coli Proteins / Q000276:immunology; D007124:Immunoenzyme Techniques; D046228:Microarray Analysis
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin