title: [Optimization of high-cell-density fermentation process for S-adenosyl-L-methionine production].
abstract: Poor stability existed in the anaphase of the high-cell-density fermentation of Saccharomyces crevisiae for S-adenosyl-L-methionine (SAM) production in 5 L fermentor. To improve the fermentation stability, we studied the addition of diammonium hydrogen phosphate, sodium glutamate and adenosine disodium triphosphate into glucose feeding solution. Study of four fed-batch cultures showed that, after 34 h fermentation, when dry cell weight reached 100 g/L, the addition of 50 g pre-L-methionine and glucose feeding with 10 g/L adenosine disodium triphosphate was optimal for SAM production. Under this condition, after 65.7 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 180 g/L and 17.1 g/L respectively.
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Jiepeng
authors.initials: J
authors.lastname: Wang
authors.forename: Jinjun
authors.initials: J
authors.lastname: Han
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Li
authors.forename: Peiyi
authors.initials: P
authors.lastname: Liu
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D005285:Fermentation; D010710:Phosphates / Q000494:pharmacology; D012436:S-Adenosylmethionine / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D012970:Sodium Glutamate / Q000494:pharmacology
chemical_list: D010710:Phosphates; C024788:ammonium phosphate; D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D012970:Sodium Glutamate