Interactions of typical and atypical enteropathogenic Escherichia coli strains with the calf intestinal mucosa ex vivo.
Enteropathogenic Escherichia coli (EPEC) can be found in healthy and diarrheic cattle; however, little is known about the role of attaching and effacing (A/E) lesion formation in colonization of bovine intestinal mucosa by such strains. We show that typical and atypical EPEC induce A/E lesions on calf intestinal explants independently of Tir tyrosine phosphorylation and TccP. Our data support the existence of conserved Tir- and TccP-independent mechanisms of A/E lesion formation in a range of hosts and reinforce the zoonotic potential of EPEC in cattle.
Girard
Francis
F
Centre for Molecular Microbiology and Infection, Division of Cell & Molecular Biology, Flowers Building, Imperial College London, London SW72AZ, United Kingdom.
Dziva
Francis
F
Stevens
Mark P
MP
Frankel
Gad
G
D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D054308:Enteropathogenic Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology; D010766:Phosphorylation
D029968:Escherichia coli Proteins; C505370:tccP protein, E coli