cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design.
Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The design of new primers allows for more precise selection of B. thuringiensis isolates, helping to better direct the programs employing biological control.
Davolos
Camila C
CC
Faculdade de Ciências Agrárias e Veterinária, Laboratório de Genética de Bactérias e Biotecnologia Aplicada, Universidade Estadual Paulista, Campus Jaboticabal, São Paulo, SP, Brazil.
Guidelli-Thuler
Ana M
AM
de Abreu
Irlan L
IL
Sena
Janete A D
JA
Lemos
Manoel V F
MV
D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000235:genetics*; D005838:Genotype; D006460:Hemolysin Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis