title: Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues.
abstract: Mesenchymal stem cells (MSCs), which evoke only minimal immune reactivity, may have anti-inflammatory and immunomodulatory effects. In this study, we conducted a comparative analysis of the immunomodulatory properties of MSCs derived from adult human tissues including bone marrow (BM), adipose tissues (AT), umbilical cord blood (CB), and cord Wharton's jelly (WJ). Using a multiple cytokine detection assay, we showed that there were no significant differences in levels of secreted factors from non-stimulated MSCs. We compared the immunosuppressive effect of BM-MSCs, AT-MSCs, CB-MSCs, and WJ-MSCs on phytohemagglutinin-induced T-cell proliferation. AT-MSCs, CB-MSCs, and WJ-MSCs effectively suppressed mitogen-induced T-cell proliferation as effectively as did BM-MSCs. Levels of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha secreted from activated T-cells increased over time, but these levels were significantly reduced when cocultured with each type of MSCs. In addition, the expression of hepatocyte growth factor, IL-10, transforming growth factor-beta(1), cyclooxygenase (COX)-1, and COX-2 were unchanged in MSCs treated with IFN-gamma and/or TNF-alpha, while indoleamine 2,3-dioxygenase (IDO) expression increased. IFN-gamma and/or TNF-alpha produced by activated T-cells were correlated with induction of IDO expression by MSCs, which, in turn, suppressed T-cell proliferation. These findings suggest that MSCs derived from AT, CB, or WJ could be substituted for BM-MSCs for treatment of allogeneic conflicts.
authors.affiliation: Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Keon Hee
authors.initials: KH
authors.lastname: Yoo
authors.forename: In Keun
authors.initials: IK
authors.lastname: Jang
authors.forename: Myoung Woo
authors.initials: MW
authors.lastname: Lee
authors.forename: Hyo Eun
authors.initials: HE
authors.lastname: Kim
authors.forename: Mal Sook
authors.initials: MS
authors.lastname: Yang
authors.forename: Youngwoo
authors.initials: Y
authors.lastname: Eom
authors.forename: Jong Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Seong Kyu
authors.initials: SK
authors.lastname: Yang
authors.forename: Hye Lim
authors.initials: HL
authors.lastname: Jung
authors.forename: Ki Woong
authors.initials: KW
authors.lastname: Sung
authors.forename: Cheol Woo
authors.initials: CW
authors.lastname: Kim
authors.forename: Hong Hoe
authors.initials: HH
authors.lastname: Koo
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D015703:Antigens, CD; D016207:Cytokines