title: Campylobacter avium sp. nov., a hippurate-positive species isolated from poultry.
abstract: Three strains of an unusual hippurate-positive Campylobacter species were isolated at 37 degrees C from caecal contents of broiler chickens and a turkey. All strains were initially identified as Campylobacter by means of genus-specific PCR, but none was further identified using specific PCRs for known thermophilic species. Phylogenetic analyses based on 16S rRNA, rpoB and groEL gene sequences revealed that these strains formed a robust clade distinct from other Campylobacter species. Amplified fragment length polymorphism analysis and whole-cell protein electrophoresis were subsequently carried out and confirmed the divergence between the avian strains and other taxa. These data indicate that the unidentified Campylobacter strains belong to a novel taxon which could be distinguished from other campylobacters through its phenotypic and genotypic characteristics. The name Campylobacter avium sp. nov., is proposed for the novel species, with the type strain 86/06T (=LMG 24591T=CCUG 56292T).
authors.affiliation: Department of Veterinary Public Health and Animal Pathology, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy. mirko.rossi@helsinki.fi
authors.forename: Mirko
authors.initials: M
authors.lastname: Rossi
authors.forename: Lies
authors.initials: L
authors.lastname: Debruyne
authors.forename: Renato Giulio
authors.initials: RG
authors.lastname: Zanoni
authors.forename: Gerardo
authors.initials: G
authors.lastname: Manfreda
authors.forename: Joana
authors.initials: J
authors.lastname: Revez
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002169:Campylobacter Infections / Q000662:veterinary*; D002432:Cecum / Q000382:microbiology; D018834:Chaperonin 60 / Q000235:genetics; D002645:Chickens; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006626:Hippurates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011200:Poultry / Q000382:microbiology*; D020543:Proteome / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D014422:Turkeys
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006626:Hippurates; D020543:Proteome; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; C030514:hippuric acid