title: Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay.
abstract: A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6)CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.
authors.affiliation: Department of Infectious Disease Control, Beijing Institute of Disease Control and Prevention, Beijing 100071, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Qu
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yufei
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhong
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Qiao
authors.forename: Xinying
authors.initials: X
authors.lastname: Du
authors.forename: Zhoujia
authors.initials: Z
authors.lastname: Wang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Zeliang
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002002:Brucella / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity