title: [Studies on chemical constituents in fruit of Alpinia oxyphylla].
abstract: OBJECTIVE
  To study the chemical constituents in fruit of Alpinia oxyphylla and their cytotoxicities on cancer cell lines.
  
  
  METHODS
  Compounds were isolated and purified by various column chromatographic methods. Their structures were determined by physico-chemical properties and spectral analyses. Compound cytotoxicity was assessed by the sulforhodamine B (SRB) assay.
  
  
  RESULTS
  Eight compounds were obtained from Me2CO-H2O (70%) extract of the fruit of A. oxyphylla and their structures were identified as: (9E)-humulene-2, 3; 6, 7-diepoxide (1), 3(12), 7(13), 9(E)-humulatriene-2, 6-diol (2), (-)-oplopanone (3), yakuchinone A (4), yakuchinone B (5), tectochrysin (6), isovanillin (7), (2E, 4E)-6-hydroxy-2, 6-dimethylhepta-2, 4-dienal (8), and the cytotoxicities of compounds 1, 3-5 on cancer cell lines, A549, HT-29 and SGC-7901, were also investigated.
  
  
  CONCLUSIONS
  Compounds 1-3, 7, 8 were isolated for the first time from this genus and compounds 1, 3-5 exhibited no cytotoxicity against three cancer cell lines at a concentration of 10 mg x L(-1).
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China. junjuxu007@126.com
authors.forename: Junju
authors.initials: J
authors.lastname: Xu
authors.forename: Ninghua
authors.initials: N
authors.lastname: Tan
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Zeng
authors.forename: Hongjin
authors.initials: H
authors.lastname: Han
authors.forename: Huoqiang
authors.initials: H
authors.lastname: Huang
authors.forename: Changjiu
authors.initials: C
authors.lastname: Ji
authors.forename: Meiju
authors.initials: M
authors.lastname: Zhu
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D006139:Guaiacol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans
chemical_list: D001547:Benzaldehydes; D036381:Diarylheptanoids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C121978:yakuchinone B; C093382:isovanillin; D006139:Guaiacol; C042566:yakuchinone-A; C402729:tectochrysin