title: Identification of volatile compounds in Chrysanthemum morifolium by microwave distillation solid-phase microextraction coupled with GC/MS.
abstract: Microwave distillation with concomitant solid-phase microextraction (MD-SPME) coupled with GC/MS was developed for identifying volatile compounds in the dried flowers of Chrysanthemum morifolium (CM). Different experimental parameters, including SPME fiber coating, microwave power, irradiation time, and water volume were optimized. The best results were obtained using carboxen/polydimethylsiloxane fiber with microwave irradiation at 400 W for 3 min and the addition of 1 mL water into 1.0 g sample. The proposed method allowed the isolation, extraction, and concentration of volatile compounds to be completed in a single step. This MD-SPME-GC/MS method has also been compared to the conventional steam distillation method followed by GC/MS for this identification. Thirty-two volatile compounds were identified using the newly developed MD-SPME-GC/MS process. Relative standard deviation values of <9.8% demonstrate good repeatability. In comparison, 27 compounds were identified by traditional steam distillation-GC/MS. Therefore, the proposed MD-SPME-GC/MS method is simple, rapid, and efficient for identifying volatile compounds in CM.
authors.affiliation: Fudan University, School of Pharmacy, Department of Pharmaceutical Analysis, Shanghai, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Bei
authors.initials: B
authors.lastname: Yang
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhang
authors.forename: Yingjia
authors.initials: Y
authors.lastname: Yu
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Yile
authors.initials: Y
authors.lastname: Chen
authors.forename: Gengli
authors.initials: G
authors.lastname: Duan
mesh_terms: D031202:Chrysanthemum / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008872:Microwaves; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D014835:Volatilization