Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material.
Orthopedic implant-related bacterial infections are associated with high morbidity that may lead to limb amputation and exert significant financial burden on the healthcare system. Staphylococcus aureus is a dominant cause of these infections, and increased incidence of community-associated methicillin-resistant S. aureus (CA-MRSA) is being reported. The ability of S. aureus to attach to the foreign body surface and develop a biofilm is an important determinant of resistance to antibiotic prophylaxis. To gain insight on CA-MRSA biofilm properties, USA300 biofilm maturation and dispersal was examined, and these biofilms were found to exhibit pronounced, quorum-sensing mediated dispersal from a glass surface. For comparison of biofilm maturation on different surface chemistries, USA300 biofilm growth was examined on glass, polycarbonate, and titanium, and minimal differences were apparent in thickness, total biomass, and substratum coverage. Importantly, USA300 biofilms grown on titanium possessed a functional dispersal mechanism, and the dispersed cells regained susceptibility to rifampicin and levofloxacin treatment. The titanium biofilms were also sensitive to proteinase K and DNaseI, suggesting the matrix is composed of proteinaceous material and extracellular DNA. These studies provide new insights on the properties of CA-MRSA biofilms on implant materials, and indicate that quorum-sensing dispersion could be an effective therapeutic strategy.
Lauderdale
Katherine J
KJ
Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA.
Malone
Cheryl L
CL
Boles
Blaise R
BR
Morcuende
Jose
J
Horswill
Alexander R
AR
D019643:Arthroplasty, Replacement / Q000295:instrumentation; D001672:Biocompatible Materials; D018441:Biofilms / Q000254:growth & development*; D017714:Community-Acquired Infections / Q000382:microbiology* / Q000517:prevention & control; D006801:Humans; D016268:Internal Fixators / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000502:physiology*; D008954:Models, Biological; D011474:Prosthesis Design; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000517:prevention & control; D053038:Quorum Sensing; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000517:prevention & control; D013499:Surface Properties; D014025:Titanium
D001672:Biocompatible Materials; D014025:Titanium