[Construction of Deinococcal bacteria-Escherichia coli shuttle vector and expression of Luciferase gene].
OBJECTIVE
To express Luciferase gene in Escherichia coli through developed Deinococcal bacteria-E. coli shuttle expression vector.


METHODS
The D. bacteria-E. coli shuttle expression vector pZT17 was constructed based on plasmids of pUE30, pGBM5 and pKatCAT. Then pZT17 with lux + from Photinus pyralis was used to transform into D. grandis and E. coli. The recombinant strains were induced separately.


RESULTS
Based on a small cryptic plasmid from Deinococcus radiopugnans, a shuttle vector between Escherichia coli and Deinococcal bacteria was constructed. The plasmid vector could stably aintained in Deinococcus grandis under non-selective conditions. Moreover, it is showed that a luciferase gene was highly expressed both observed in D. grandis and E. coli.


CONCLUSIONS
The D. bacteria-E. coli shuttle vector was constructed successfully, the developed shuttle vector makes it possible to induce expression of DNA damage and repair gene from Deinococcus species.
Tu
Zhenli
Z
College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. tuzl@zju.edu.cn
Zhong
Rujie
R
Wang
Jiagang
J
D003001:Cloning, Molecular; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000032:analysis; D034301:Deinococcus / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics*
D004269:DNA, Bacterial; D008156:Luciferases