LIF-free embryonic stem cell culture in simulated microgravity.
BACKGROUND
Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.


RESULTS
We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.


CONCLUSIONS
Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
Kawahara
Yumi
Y
Department of Clinical Neuroscience and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan.
Manabe
Tomotaka
T
Matsumoto
Masaya
M
Kajiume
Teruyuki
T
Matsumoto
Masayasu
M
Yuge
Louis
L
D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D017931:DNA Primers; D053595:Embryonic Stem Cells / Q000166:cytology*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D014893:Weightlessness
D017931:DNA Primers; D053605:Leukemia Inhibitory Factor