title: An integrated microfluidic device for large-scale in situ click chemistry screening.
abstract: An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.
authors.affiliation: Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 570 Westwood Plaza, building 114, Los Angeles, CA 90095, USA.
authors.forename: Yanju
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Lin
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Lin
authors.forename: Matthias
authors.initials: M
authors.lastname: Selke
authors.forename: Hartmuth C
authors.initials: HC
authors.lastname: Kolb
authors.forename: Nangang
authors.initials: N
authors.lastname: Zhang
authors.forename: Xing-Zhong
authors.initials: XZ
authors.lastname: Zhao
authors.forename: Michael E
authors.initials: ME
authors.lastname: Phelps
authors.forename: Clifton K F
authors.initials: CK
authors.lastname: Shen
authors.forename: Kym F
authors.initials: KF
authors.lastname: Faull
authors.forename: Hsian-Rong
authors.initials: HR
authors.lastname: Tseng
mesh_terms: D000114:Acetylene / Q000737:chemistry; D000818:Animals; D001386:Azides / Q000737:chemistry; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors / Q000378:metabolism; D002257:Carbonic Anhydrase Inhibitors / Q000032:analysis / Q000494:pharmacology; D002417:Cattle; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D018511:Systems Integration
chemical_list: D001386:Azides; D002257:Carbonic Anhydrase Inhibitors; D024402:Carbonic Anhydrase II; D000114:Acetylene