Fasting and postprandial relationships among plasma leptin, ghrelin, and insulin in prepubertal obese children.
BACKGROUND
Leptin is involved in the long-term regulation of body weight and dietary intake, while ghrelin plays an essential role in appetite control. High levels of leptin have been associated with adiposity and the suppression of ghrelin levels with increased dietary intake.


OBJECTIVE
To evaluate fasting and postprandial concentrations of plasma leptin and ghrelin after intake of a standardised breakfast and to study the relationship of these hormones with adiposity and insulin resistance in obese prepubertal children.


METHODS
34 obese and 20 normal-weight prepubertal children aged 6-12 years were selected. Plasma leptin and ghrelin were measured by ELISA and radioimmunoassay, respectively. The general linear model of variance, principal-component factor, and Pearson's analyses correlation were performed.


RESULTS
Baseline and postprandial leptin levels were higher in obese versus normal-weight children. In obese, ghrelin showed an altered pattern during the postprandial period, recovering to baseline levels at 3h after the intake. Insulin resistance was associated with leptin and independently with ghrelin.


CONCLUSIONS
The association of ghrelin with insulin resistance provides further evidence on the regulation of ghrelin in glucose homeostasis in childhood obesity at the prepubertal age. Changes in ghrelin after dietary intake may be related to an earlier recovery of appetite in prepubertal obese children.
Gil-Campos
M
M
Unit of Paediatric Endocrinology, Reina Sofia University Hospital, Avda Menéndez Pidal s/n, 14004 Córdoba, Spain. mercedes_gil_campos@yahoo.es
Aguilera
C M
CM
Ramirez-Tortosa
M C
MC
Cañete
R
R
Gil
A
A
D050154:Adiposity; D000886:Anthropometry / Q000379:methods; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure; D002648:Child; D004032:Diet / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005215:Fasting / Q000097:blood*; D005260:Female; D054439:Ghrelin / Q000032:analysis / Q000097:blood*; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood*; D019518:Postprandial Period; D025341:Principal Component Analysis; D011863:Radioimmunoassay
D015415:Biomarkers; D054439:Ghrelin; D007328:Insulin; D020738:Leptin