[Development of single base extension-tags microarray for the detection of food-borne pathogens].
We developed single base extension-tags (SBE-tags) microarray to detect eight common food-borne pathogens, including Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella, Enterobacter sakazaki, Shigella, Escherichia coli O157:H7 and Campylobacter jejuni. With specific PCR primers identified and integrated for eight food-borne pathogens, target sequences were amplified and purified as template DNA of single base extension-tags reaction. The products were hybridized to microarrays and scanned for fluorescence intensity. The experiment showed a specific and simultaneous detection of eight food-borne pathogens. The system limits is 0.1 pg for a genomic DNA and 5x10(2) CFU/mL for Salmonella typhimurium cultures. The single base extension-tags assay can be used to detect food-borne pathogens rapidly and accurately with a high sensitivity, and provide an efficient way for diagnosis and control of disease caused by food-borne pathogens.
Lu
Changyong
C
Department of Food Science and Bor Luh Food Safety Center, School ofAgriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Shi
Chunlei
C
Zhang
Chunxiu
C
Chen
Jing
J
Shi
Xianming
X
D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification