title: Extrinsic surgical denervation ameliorates TNBS-induced colitis in rats.
abstract: OBJECTIVE
  Neurogenic inflammation refers to an inflammatory reflex arc by sensory neurons which transmit nocious stimulus centrally and results in both pain perception and intense local inflammatory reaction. Specific neurons, receptors, and their respective neurotransmitters have been studied in numerous organ systems including the gastrointestinal tract. Neurogenic inflammation has been suggested to play a key role in the pathogenesis of inflammatory bowel disease. In this study, we studied the effect of surgical denervation of specific somatosensory neurons in a well-established animal model of colitis.
  
  
  METHODS
  Adult male rats were underwent surgical denervation around the inferior mesenteric artery or sham operation. After ten days trinitrobenzene sulfonic acid (TNBS) or vehicle was administered by enema. Inflammation was assessed by, histological evaluation, macroscopic damage score, myeloperoxidase (MPO) activity, and substance P receptor immunoreactivity (SPRIR).
  
  
  RESULTS
  Compared with sham operation with TNBS administration, surgical denervation with TNBS administration suppressed the score in all of the inflammatory indices and had almost no signs of inflammation in histological evaluation.
  
  
  CONCLUSIONS
  Surgical denervation has a protective effect on TNBS-induced colitis in rats. Thus, sensory neurons play a key role in the pathogenesis of inflammation in this well-established model of acute colitis.
authors.affiliation: Department of General Surgery, National Hospital Organization Chiba Medical Center, Chiba, Japan. yoji-t@kd5.so-net.ne.jp
authors.forename: Yoji
authors.initials: Y
authors.lastname: Takami
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Mantyh
authors.forename: Theodore N
authors.initials: TN
authors.lastname: Pappas
authors.forename: Toku
authors.initials: T
authors.lastname: Takahashi
authors.forename: Keiji
authors.initials: K
authors.lastname: Koda
authors.forename: Masaru
authors.initials: M
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology / Q000628:therapy*; D003106:Colon / Q000294:innervation*; D003714:Denervation / Q000379:methods*; D004195:Disease Models, Animal; D004210:Dissection; D008297:Male; D019800:Phenol / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D018040:Receptors, Neurokinin-1 / Q000502:physiology; D012597:Sclerosing Solutions / Q000008:administration & dosage; D013373:Substance P / Q000502:physiology; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D018040:Receptors, Neurokinin-1; D012597:Sclerosing Solutions; D013373:Substance P; D019800:Phenol; D014302:Trinitrobenzenesulfonic Acid