title: Label-free DNA sensor based on fluorescent cationic polythiophene for the sensitive detection of hepatitis B virus oligonucleotides.
abstract: Water-soluble fluorescent conjugated polymers can be used as an optical platform in highly sensitive DNA sensors. Here we report a simple label-free DNA sensor using poly(3-alkoxy-4-methylthiophene) to recognize and detect different oligonucleotide targets related to the YMDD gene mutation of hepatitis B virus. The concentration of surfactant Triton X-100, NaCl, the oligonucleotide capture probe and the oligonucleotide hybridization conditions have a great impact on fluorescence intensity. Under the optimum conditions, two types of oligonucleotide targets involving YMDD gene mutation of hepatitis B virus were successfully recognized. Moreover, there was a linear relationship between fluorescence intensity and the concentration of oligonucleotide target. The detection limit of the wild-type hepatitis B virus target is 88 pmol L(-1).
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Guan
authors.forename: Min
authors.initials: M
authors.lastname: Cai
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
mesh_terms: D002412:Cations / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005453:Fluorescence; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification*; D009154:Mutation; D015345:Oligonucleotide Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis* / Q000235:genetics
chemical_list: D002412:Cations; D015342:DNA Probes; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D009841:Oligonucleotides