A new synthetic compound, 2-OH, enhances interleukin-2 and interferon-gamma gene expression in human peripheral blood mononuclear cells.
A new synthetic compound, 6-hydroxy-2-tosylisoquinolin-1(2H)-one (2-OH), was selected for immunopharmacological activity tests. The effects of 2-OH on human peripheral blood mononuclear cell (PBMC) proliferation were determined by tritiated thymidine uptake. Compared to phytohemagglutinin (PHA; 5 microg/mL) stimulation, 2-OH significantly enhanced PBMC proliferation in a dose-dependent manner. The 50% enhancement activity (EC(50)) for 2-OH was 4.4+/-0.1 microM. In addition, effects of 2-OH on interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production in PBMC were determined by enzyme immunoassay. Results demonstrated that 2-OH stimulated IL-2 and IFN-gamma production in PBMC. Data from reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR indicated that IL-2 and IFN-gamma mRNA expression in PBMC could be induced by 2-OH. Therefore, 2-OH enhanced IL-2 and IFN-gamma production in PBMC by modulation their gene expression. We suggest that 2-OH may be an immunomodulatory agent.
Chou
Shiu-Huey
SH
Department of Life Science, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan. 047751@mail.fju.edu.tw
Chou
Shang-Shing P
SS
Liew
Yih-Fong
YF
Leu
Jyh-Yih
JY
Wang
Su-Jane
SJ
Huang
Rwei-Fen S
RF
Tzeng
Woan-Fang
WF
Kuo
Yuh-Chi
YC
D049109:Cell Proliferation; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007155:Immunologic Factors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D015363:Quinolones / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
C544084:6-hydroxy-2-tosylisoquinolin-1(2H)-one; D007155:Immunologic Factors; D007376:Interleukin-2; D015363:Quinolones; D007371:Interferon-gamma