title: Anaplasma phagocytophilum and Anaplasma marginale elicit different gene expression responses in cultured tick cells.
abstract: The genus Anaplasma (Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms, Anaplasma phagocytophilum and Anaplasma marginale that multiply in both vertebrate and tick host cells. Recently, we showed that A. marginale affects the expression of tick genes that are involved in tick survival and pathogen infection and multiplication. However, the gene expression profile in A. phagocytophilum-infected tick cells is currently poorly characterized. The objectives of this study were to characterize tick gene expression profile in Ixodes scapularis ticks and cultured ISE6 cells in response to infection with A. phagocypthilum and to compare tick gene expression responses in A. phagocytophilum- and A. marginale-infected tick cells by microarray and real-time RT-PCR analyses. The results of these studies demonstrated modulation of tick gene expression by A. phagocytophilum and provided evidence of different gene expression responses in tick cells infected with A. phagocytophilum and A. marginale. These differences in Anaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.
authors.affiliation: Utrecht Centre for Tick-Borne Diseases (UCTD), Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Zivkovic
authors.forename: Edmour F
authors.initials: EF
authors.lastname: Blouin
authors.forename: Raúl
authors.initials: R
authors.lastname: Manzano-Roman
authors.forename: Consuelo
authors.initials: C
authors.lastname: Almazán
authors.forename: Victoria
authors.initials: V
authors.lastname: Naranjo
authors.forename: Robert F
authors.initials: RF
authors.lastname: Massung
authors.forename: Frans
authors.initials: F
authors.lastname: Jongejan
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Kocan
authors.forename: José
authors.initials: J
authors.lastname: de la Fuente