title: Anti-epidermal growth factor receptor (anti-EGFR) antibody conjugated fluorescent nanoparticles probe for breast cancer imaging.
abstract: Fluorescent nanoparticles (FNs) with unique optical properties may be useful as biosensors in living cancer cell imaging and cancer targeting. In this study, anti-EGFR antibody conjugated fluorescent nanoparticles (FNs) (anti-EGFR antibody conjugated FNs) probe was used to detect breast cancer cells. FNs with excellent character such as non-toxicity and photostability were first synthesized with a simple, cost-effective and environmentally friendly modified Stober synthesis method, and then successfully modified with anti-EGFR antibody. This kind of fluorescence probe based on the anti-EGFR antibody conjugated FNs has been used to detect breast cancer cells with fluorescence microscopy imaging technology. The experimental results demonstrate that the anti-EGFR antibody conjugated FNs can effectively recognize breast cancer cells and exhibited good sensitivity and exceptional photostability, which would provide a novel way for the diagnosis and curative effect observation of breast cancer cells and offer a new method in detecting EGFR.
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. hunxu@sohu.com
authors.forename: Xu
authors.initials: X
authors.lastname: Hun
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000276:immunology*; D005260:Female; D006801:Humans; D008856:Microscopy, Fluorescence / Q000191:economics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D055910:Antibodies, Immobilized; D066246:ErbB Receptors