[Correlation of genetic polymorphisms of beta-catenin to hepatitis B virus-related hepatocellular carcinoma].
OBJECTIVE
Wnt signaling pathway plays an important role in the carcinogenesis of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). beta-catenin protein is a pivotal regulator in the pathway. Genetic mutation has been observed in the codons 32-45 at exon 3 of beta-catenin gene in HCC tissues. This study was to investigate the correlation of genetic polymorphisms of beta-catenin to HBV-related HCC.


METHODS
We conducted epidemiologic and genetic investigation in 162 patients with HBV-related HCC. According to matching requirements, patients with or without family history of HCC were paired with a ratio of 1:1. Exon 3 of beta-catenin gene was detected by polymerase chain reaction (PCR) and DNA sequencing. Single nucleotide polymorphism (SNP) rs28931588, rs28931589, codons 31-46 sequences and genetic investigation were analyzed.


RESULTS
Among the 162 HCC patients, 16 (9.88%) had family history of HCC; 12 patients with family history of HCC and 12 without family history were matched. The 24 patients all showed G on rs28931588 and rs28931589. Three patients showed mutation in codons 32-46 and had genetic polymorphisms. Definite mutational regularity or characteristic mutational site was not observed.


CONCLUSIONS
SNP rs28931588, rs28931589 and codons 31-46 sequences may not be the genetic markers in HBV-related HCC.
Lai
Jing
J
Department of Infection Diseases, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510630, P.R. China.
Gao
Zhi-Liang
ZL
Yang
Lin
L
Chen
Wei
W
Zhou
Xiang
X
Ke
Wei-Min
WM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D003062:Codon; D005091:Exons; D005260:Female; D006509:Hepatitis B; D006515:Hepatitis B virus / Q000302:isolation & purification; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
C495270:CTNNB1 protein, human; D003062:Codon; D051176:beta Catenin