[The first report of Kadipiro virus isolation in China].
5 strains of virus isolated from Culex tritaeniorhynchus, Anopheles sinensis and Armigeres subalbatus, which caused cytopathic effect in C6/36 cells, had been obtained in the survey of arboviruses in Northwestern Yunnan Province. China. The virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. RNA-PAGE of the genomes of the isolates showed 6-5-1 profile. A fragment of the 12th segment sequence was amplified by a pair of specific primers for Kadipiro virus strain JKT-7075 in RT-PCR. The full length of the 12th segment was 758 nucleotides, BLAST analysis revealed the highest identity was 90% to JKT-7075. Phylogenetic analysis demonstrated that the isolates appeared to be Kadipiro viruses (Family Reoviridae). It was the first report of kadipiro virus isolation in China.
Sun
Xiao-hong
XH
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. dotsunny@hotmail.com
Meng
Wei-shan
WS
Fu
Shi-hong
SH
Feng
Yun
Y
Zhai
You-gang
YG
Wang
Jing-lin
JL
Wang
Huan-qin
HQ
Lv
Xin-jun
XJ
Liang
Guo-dong
GD
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000821:virology; D002460:Cell Line; D002681:China; D018162:Coltivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003465:Culex / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA