NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster.
We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo-Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.
Pauleta
Sofia R
SR
REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica 2829-516, Portugal. srp@dq.fct.unl.pt
Duarte
Américo G
AG
Carepo
Marta S
MS
Pereira
Alice S
AS
Tavares
Pedro
P
Moura
Isabel
I
Moura
José J G
JJ
D001059:Apoproteins / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D002247:Carbon Isotopes; D003300:Copper / Q000737:chemistry; D045286:Desulfovibrio gigas / Q000737:chemistry* / Q000235:genetics; D015394:Molecular Structure; D008982:Molybdenum / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
D001059:Apoproteins; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D011994:Recombinant Proteins; D003300:Copper; D008982:Molybdenum