Photonic immobilization of high-density protein arrays using Fourier optics.
The technique of UV-light-assisted immobilization of disulfide containing proteins has been combined with the Fourier-transforming properties of lenses as well as with a simple millimeter scale feature size spatial mask. The result is a new simple and inexpensive way of creating high-density protein arrays with feature sizes down to a few hundred nanometers, which represents an improvement of tenfold over existing commercially available high-density protein arraying methods.
Skovsen
Esben
E
Department of Physics and Nanotechnology, Aalborg University, Denmark.
Kold
Ane B
AB
Neves-Petersen
Maria Teresa
MT
Petersen
Steffen B
SB
D004220:Disulfides / Q000737:chemistry*; D005583:Fourier Analysis; D055767:Immobilized Proteins / Q000032:analysis* / Q000737:chemistry; D055095:Optics and Photonics; D040081:Protein Array Analysis / Q000191:economics / Q000379:methods*; D014466:Ultraviolet Rays
D004220:Disulfides; D055767:Immobilized Proteins