[Human papillomavirus 16E6 gene mutation in cervical carcinoma tissues].
OBJECTIVE
Human papillomavirus (HPV) 16 is the most common type of high-risk human HPVs. HPV16 E6 gene and its specific mutations are considered as risk factors causing cervical carcinoma (CC). This study was to investigate HPV16 E6 mutations in Lanzhou region and explore the relationship between HPV16 E6 mutations and the development of CC.


METHODS
Tissue DNA was extracted from 23 patients operated on for CC and five normal cervical controls. The partial sequence of the HPV16 E6 gene (nucleotide 201-523) was amplified by PCR from the tissue DNA extracted from the samples. PCR fragments were sequenced and analyzed.


RESULTS
The positive rates of HPV16 E6 in five normal cervical and 23 CC tissues were 0 (0/5) and 82.61% (19/23), respectively. Prototype HPV16E6 gene was found in six cases (33.33%) while mutation in the E6 gene was detected in 12 cases (66.67%), among which a 350G mutation was found in 11 cases (61.11%). Moreover, a 249G mutation was identified in one CC case (5.56%).


CONCLUSIONS
There is a high HPV infection rate in CC tissues in Lanzhou region, and most of the HPV16E6 are mutated.
Sun
Feng
F
Department of Pathology, Lanzhou General Hospital of PLA, Lanzhou, Gansu, China.
Ha
Xiao-Qin
XQ
Lv
Tong-De
TD
Xing
Chuan-Ping
CP
Liu
Bin
B
Cao
Xiao-Zhe
XZ
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005814:Genes, Viral; D052162:Human papillomavirus 16 / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics*; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult
D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins