title: Effect of excessive acute-phase response on liver regeneration after partial hepatectomy in rats.
abstract: OBJECTIVE
  Acute-phase response (APR) is known to have a protective role against infection and tissue injury including partial hepatectomy (HTX). In this study, the effect of excessive APR on liver regeneration was investigated using a rat hepatectomized model.
  
  
  METHODS
  Experimental APR was induced by subcutaneous injection of turpentine oil, immediately, 24 h and 48 h after 50% HTX. Serum IL-6 and hepatic alpha2-macroglobulin mRNA was evaluated in rats receiving turpentine or saline as control after HTX. DNA synthesis and PCNA labeling index in the remnant liver was also investigated. Furthermore, in vitro total hepatic protein synthesis (TPS) and secretory protein synthesis (SPS) in the remnant liver were measured.
  
  
  RESULTS
  Liver regeneration was significantly inhibited after HTX in the turpentine group, compared to the control. DNA synthesis and PCNA labeling index was also significantly decreased on days 1 and 2. A remarkable increase in the serum IL-6 and hepatic alpha2-macroglobulin mRNA was found in the turpentine group. Furthermore, SPS was significantly upregulated in the turpentine group, although TPS was almost similar between the groups.
  
  
  CONCLUSIONS
  Induction of excessive APR after HTX inhibits liver regeneration. Acute phase protein synthesis is remarkably upregulated within the capacity of hepatic protein synthesis, most probably by a reduction in replicative protein synthesis, suggesting that APR may be more appropriate biological response for ensuring immediate survival.
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Kusashio
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Fumio
authors.initials: F
authors.lastname: Kimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshidome
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kato
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yoshitomi
authors.forename: Katsunori
authors.initials: K
authors.lastname: Furukawa
authors.forename: Tadaomi
authors.initials: T
authors.lastname: Fukada
authors.forename: Masaru
authors.initials: M
authors.lastname: Miyazaki
mesh_terms: D000210:Acute-Phase Reaction / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology*; D000818:Animals; D004195:Disease Models, Animal; D006498:Hepatectomy; D015850:Interleukin-6 / Q000097:blood; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D009929:Organ Size; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D018809:Proliferating Cell Nuclear Antigen; D000511:alpha-Macroglobulins; D014425:Turpentine