title: The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii.
abstract: Here we report the characterization of the Chlamydomonas reinhardtii gene ARG9, encoding the plastid resident N-acetyl ornithine aminotransferase, which is involved in arginine synthesis. Integration of an engineered ARG9 cassette in the plastid chromosome of the nuclear arg9 mutant restores arginine prototrophy. This suggests that ARG9 could be used as a new selectable marker for plastid transformation.
authors.affiliation: Department of Life Sciences, Université de Liège, Liège, Belgium.
authors.forename: Claire
authors.initials: C
authors.lastname: Remacle
authors.forename: Sara
authors.initials: S
authors.lastname: Cline
authors.forename: Layla
authors.initials: L
authors.lastname: Boutaffala
authors.forename: Stéphane
authors.initials: S
authors.lastname: Gabilly
authors.forename: Véronique
authors.initials: V
authors.lastname: Larosa
authors.forename: M Rosario
authors.initials: MR
authors.lastname: Barbieri
authors.forename: Nadine
authors.initials: N
authors.lastname: Coosemans
authors.forename: Patrice P
authors.initials: PP
authors.lastname: Hamel
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics / Q000378:metabolism*; D018087:Plastids / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport
chemical_list: D020418:Algal Proteins; D001120:Arginine; D009953:Ornithine-Oxo-Acid Transaminase