Involvement of sensory nerves in the protective effect of growth hormone on acute pancreatitis.
Growth hormone (GH) has been shown to protect the intestinal barrier integrity and to stimulate the production of insulin-like growth factor 1 (IGF-1), which inhibits the development of acute pancreatitis. Sensory nerves are implicated in the protection of pancreatic tissue against acute inflammation. The aim of this study was to investigate the influence of exogenous GH on acute pancreatitis (AP) and to assess the involvement of sensory nerves and IGF-1 in above effect. Studies were performed on Wistar rats. AP was induced by subcutaneous administration of caerulein (25mug/kg) to the conscious animals. GH (1 or 2mg/kg) was administered to the rats as an intraperitoneal injection 30min prior to the start of AP. To deactivate sensory nerves capsaicin was given at total dose of 100mg/kg 10days before the experiments. AP was confirmed by histological examination and manifested by the significant rises of pancreatic weight, and serum activities of lipase, TNFalpha and IL-10 (by 550%, 300% and 50%, respectively), whereas IGF-1 blood concentration was markedly reduced. Administration of GH prior to the caerulein infusion significantly increased GH, IGF-1 and IL-10 blood levels, attenuated harmful effects of AP and reduced histological manifestations of pancreatitis in the rats with intact sensory nerves. This was accompanied by the reduction of serum lipase, and TNFalpha activities. In the AP rats with capsaicin-deactivated sensory nerves GH failed to protect the pancreas against acute damage and, as a consequence of above deactivation, IGF-1 was low.


CONCLUSIONS
GH modulates the development of acute pancreatitis in the presence of active sensory nerves probably via stimulation of IGF-1 release.
Jaworek
Jolanta
J
Department of Medical Physiology, Faculty of Health Sciences, Jagiellonian University School of Medicine Cracow, Poland. mpjawore@cyf-kr.edu.pl
Leja-Szpak
Anna
A
Dembiński
Artur
A
Tomaszewska
Romana
R
Szklarczyk
Joanna
J
Kot
Michalina
M
Nawrot-Porabka
Katarzyna
K
Bonior
Joanna
J
Warzecha
Zygmunt
Z
Pawlik
Wiesław W
WW
D000208:Acute Disease; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D008954:Models, Biological; D010179:Pancreas / Q000294:innervation; D010195:Pancreatitis / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000378:metabolism*; D018689:Sensory System Agents / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D018689:Sensory System Agents; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D002211:Capsaicin