title: A new and efficient method for inhibition of RNA viruses by DNA interference.
abstract: We report here a new method for inhibition of RNA viruses induced by dsDNA. We demonstrated that both long dsDNA molecules and short interfering DNA with a sequence complementary to that of viral RNA inhibited tobacco mosaic virus expression and prevented virus spread. Also, the expression of the HIV-1 gp41 gene in HeLa cells was inhibited by complementary short interfering DNA. We showed that Dicer processed dsDNA, which suggests activation of the cellular machinery involved in silencing of RNA. For the silencing of viral RNA effected with dsDNA, we coined the term DNA interference technology.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Nowak
authors.forename: Eliza
authors.initials: E
authors.lastname: Wyszko
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Fedoruk-Wyszomirska
authors.forename: Henryk
authors.initials: H
authors.lastname: Pospieszny
authors.forename: Mirosława Z
authors.initials: MZ
authors.lastname: Barciszewska
authors.forename: Jan
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D053487:DEAD-box RNA Helicases / Q000502:physiology; D016373:DNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D020868:Gene Silencing / Q000187:drug effects*; D005814:Genes, Viral / Q000187:drug effects*; D015700:HIV Envelope Protein gp41 / Q000037:antagonists & inhibitors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008722:Methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D012328:RNA Viruses / Q000187:drug effects* / Q000235:genetics; D012367:RNA, Viral / Q000037:antagonists & inhibitors*; D043244:Ribonuclease III / Q000502:physiology; D014027:Tobacco Mosaic Virus / Q000187:drug effects / Q000235:genetics
chemical_list: D016373:DNA, Antisense; D015700:HIV Envelope Protein gp41; D020319:Oligodeoxyribonucleotides, Antisense; D012367:RNA, Viral; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases