Microfluidic enrichment of a target cell type from a heterogenous suspension by adhesion-based negative selection.
The enrichment or isolation of a selected type of cells in a heterogeneous suspension is challenging when the surface markers of these cells are not completely known. Here, we present a 3-stage arrangement of peptide-coated microfluidic channels that can recover a small number of adipose-derived stem cells (ADSCs) from a heterogenous suspension by negative selection depletion of the non-target cell types.
Green
James V
JV
Department of Chemical Engineering, Northeastern University, Boston, Massachusetts 02115, USA.
Murthy
Shashi K
SK
D000273:Adipose Tissue / Q000166:cytology; D002448:Cell Adhesion / Q000502:physiology; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D009130:Muscle, Smooth / Q000166:cytology; D009842:Oligopeptides / Q000737:chemistry; D033081:Shear Strength; D013234:Stem Cells / Q000166:cytology
D009842:Oligopeptides