title: Diagnostic and prognostic utility of serum receptor-binding cancer antigen expressed on SiSo cells (RCAS1) levels in colon cancer patients.
abstract: Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a human tumor-associated antigen that induces cell-cycle arrest and/or apoptosis in cells bearing the RCAS1 receptor. The aim of the present study was to elucidate the diagnostic and prognostic utility of RCAS1 levels in colon cancer patients. Serum RCAS1 levels were determined using a sandwich enzyme-linked immunosorbent assay in 97 colon cancer patients and 20 healthy individuals. The levels were significantly increased in colon cancer patients compared to healthy individuals (p<0.0001). Increased RCAS1 levels were significantly associated with advanced Dukes' stage (p=0.0079) and high histopathological tumor grade (p=0.0028). Univariate analysis revealed that colon cancer patients with elevated RCAS1 levels had significantly shorter overall survival times (log-rank test, p=0.027). By multivariate analysis, serum RCAS1 was identified as an independent prognostic factor (Cox regression analysis, p=0.033). In conclusion, colon cancer patients with advanced disease stage and grade and poor prognosis showed elevated serum RCAS1 levels. Assessment of serum RCAS1 levels could therefore be considered as a diagnostic and prognostic marker in colon neoplasia.
authors.affiliation: Department of Forensic Medicine and Toxicology, Medical School, University of Athens, Athens, Greece.
authors.forename: Costas
authors.initials: C
authors.lastname: Giaginis
authors.forename: Alexandra
authors.initials: A
authors.lastname: Margeli
authors.forename: Gregory
authors.initials: G
authors.lastname: Kouraklis
authors.forename: Athina
authors.initials: A
authors.lastname: Zira
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Tsourouflis
authors.forename: Stamatios
authors.initials: S
authors.lastname: Theocharis
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D014408:Biomarkers, Tumor; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D055088:Early Detection of Cancer; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C120762:EBAG9 protein, human