title: Regulation of ATGL expression mediated by leptin in vitro in porcine adipocyte lipolysis.
abstract: Adipose triglyceride lipase (ATGL), as an adipose-enriched protein, is able to hydrolyze triglycerides and plays an important part in triglyceride lipolysis of fat tissue. Leptin, an adipocyte cytokine, can increase the fat decomposition process. Many phenomena indicate that ATGL has a close relationship with leptin's promoting the hydrolysis of triglycerides. However, the regulatory mechanism of ATGL in leptin's promoting fat hydrolysis has not been directly and systematically studied yet. This study demonstrated that ATGL was expressed in vitro by leptin regulation. The amount of ATGL mRNA increased and the amount of ATGL protein decreased based on a dose-dependent manner when leptin concentrations ranged from 5 to 50 ng/ml were used to treat fully differentiated porcine adipocytes for 3 h. In addition, this study revealed that JAK-STAT and MAPK signaling pathways, as well as PPAR gamma all played important roles in the ATGL expression mediated by leptin.
authors.affiliation: Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Yu-cheng
authors.initials: YC
authors.lastname: Li
authors.forename: Xue-li
authors.initials: XL
authors.lastname: Zheng
authors.forename: Bing-ting
authors.initials: BT
authors.lastname: Liu
authors.forename: Gong-she
authors.initials: GS
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D020738:Leptin / Q000494:pharmacology*; D008049:Lipase / Q000032:analysis / Q000235:genetics*; D008066:Lipolysis; D048670:MAP Kinase Kinase 4; D020935:MAP Kinase Signaling System; D047495:PPAR gamma; D012333:RNA, Messenger / Q000032:analysis; D050791:STAT Transcription Factors; D015398:Signal Transduction; D013552:Swine
chemical_list: D020738:Leptin; D047495:PPAR gamma; D012333:RNA, Messenger; D050791:STAT Transcription Factors; D048670:MAP Kinase Kinase 4; D008049:Lipase