A liquid chromatography/tandem mass spectrometry method for determination of aristolochic acid-I in rat plasma.
A sensitive, rapid and specific liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed and validated for the determination of aristolochic acid-I (AA-I) in rat plasma. Finasteride was used as the internal standard (IS). The analyte was separated on a Zorbax Eclipse XDB-C(18) column by isocratic elution with methanol-10 mM ammonium acetate (75:25, v/v, pH = 7.3) at a flow rate of 0.25 mL/min, and analyzed by mass spectrometry in positive multiple reaction monitoring mode. The precursor-to-product ion transitions of m/z 359.0 --> 298.2 and m/z 373.1 --> 305.2 were used to detect AA-I and IS, respectively. Good linearity was achieved over a range of 0.4-600 ng/mL. Intra- and inter-day precisions measured as relative standard deviation were less than 13.5%, and accuracy ranged from 94.2 to 97.5%. The developed method was successfully applied in the pharmacokinetic study of AA-I in rats.
Liu
Yiming
Y
Guangdong Provincial Hospital of TCM Affiliated to Guangzhou University of TCM, 111 Dade Road, Guangzhou 510120, People's Republic of China. lyming2000@163.com
Lin
Aihua
A
Wu
Zhifeng
Z
Ou
Runmei
R
Huang
Haiding
H
D000818:Animals; D019540:Area Under Curve; D034341:Aristolochic Acids / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000097:blood; D018120:Finasteride / Q000097:blood; D006207:Half-Life; D007202:Indicators and Reagents; D007441:Intubation, Gastrointestinal; D010949:Plasma / Q000737:chemistry; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D053719:Tandem Mass Spectrometry
D034341:Aristolochic Acids; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D012996:Solutions; D018120:Finasteride; C000228:aristolochic acid I