Improved isolation of glucuronan from algae and the production of glucuronic acid oligosaccharides using a glucuronan lyase.
A new source for the production of bioactive glucuronic acid oligosaccharides (GlcUAOs) from the depolymerization of green seaweed Ulva lactuca glucuronan (Algal glucuronan) has been investigated. Algal glucuronan purification was optimized by the acidic precipitation method which allowed us to separate the polysaccharide mixture extracted from the cell wall of Ulvalactuca using hot water containing sodium oxalate. A series of the GlcUAOs were obtained by enzyme degradation of algal glucuronan with a glucuronan lyase (GL) isolated from Trichoderma strain. The putative bioactive GlcUAOs generated were then purified by size-exclusion chromatography in gram quantity and characterized by (1)H/(13)C NMR spectroscopy and ESI-Q/TOF-mass spectrometry.
Redouan
Elboutachfaiti
E
LPMV, Université de Picardie Jules Verne, IUT-GB Amiens, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France. redouan_elboutachfaiti@hotmail.com
Cedric
Delattre
D
Emmanuel
Petit
P
Mohamed
El Gadda
el G
Bernard
Courtois
C
Philippe
Michaud
M
Cherkaoui
El Modafar
el M
Josiane
Courtois
C
D002240:Carbohydrate Sequence; D003365:Costs and Cost Analysis; D020723:Glucuronic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000378:metabolism*; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology; D044445:Ulva / Q000737:chemistry*
D009844:Oligosaccharides; D011134:Polysaccharides; C088556:mucoric acid; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase