title: Coenzyme Q10 supplementation lowers hepatic oxidative stress and inflammation associated with diet-induced obesity in mice.
abstract: BACKGROUND
  Diabetes and obesity are metabolic disorders induced by an excessive dietary intake of fat, usually related to inflammation and oxidative stress.
  
  
  OBJECTIVE
  The aim of the study is to investigate the effect of the antioxidant coenzyme Q10 (CoQ10) on hepatic metabolic and inflammatory disorders associated with diet-induced obesity and glucose intolerance.
  
  
  METHODS
  C57bl6/j mice were fed for 8 weeks, either a control diet (CT) or a high-fat diet plus 21% fructose in the drinking water (HFF). CoQ10 supplementation was performed in this later condition (HFFQ).
  
  
  RESULTS
  HFF mice exhibit increased energy consumption, fat mass development, fasting glycaemia and insulinemia and impaired glucose tolerance. HFF treatment promoted the expression of genes involved in reactive oxygen species production (NADPH oxidase), inflammation (CRP, STAMP2) and metabolism (CPT1alpha) in the liver. CoQ10 supplementation decreased the global hepatic mRNA expression of inflammatory and metabolic stresses markers without changing obesity and tissue lipid peroxides compared to HFF mice. HFF diets paradoxically decreased TBARS (reflecting lipid peroxides) levels in liver, muscle and adipose tissue versus CT group, an effect related to vitamin E content of the diet.
  
  
  CONCLUSIONS
  In conclusion, HFF model promotes glucose intolerance and obesity by a mechanism independent on the level of tissue peroxides. CoQ10 tends to decrease hepatic stress gene expression, independently of any modulation of lipid peroxidation, which is classically considered as its most relevant effect.
authors.affiliation: Louvain Drug Research Institute, Nutrition and Metabolism Research Group, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Florence M
authors.initials: FM
authors.lastname: Sohet
authors.forename: Audrey M
authors.initials: AM
authors.lastname: Neyrinck
authors.forename: Barbara D
authors.initials: BD
authors.lastname: Pachikian
authors.forename: Fabienne C
authors.initials: FC
authors.lastname: de Backer
authors.forename: Laure B
authors.initials: LB
authors.lastname: Bindels
authors.forename: Petra
authors.initials: P
authors.lastname: Niklowitz
authors.forename: Thomas
authors.initials: T
authors.lastname: Menke
authors.forename: Patrice D
authors.initials: PD
authors.lastname: Cani
authors.forename: Nathalie M
authors.initials: NM
authors.lastname: Delzenne
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000008:administration & dosage*; D004734:Energy Metabolism / Q000187:drug effects; D005632:Fructose / Q000008:administration & dosage; D005947:Glucose / Q000378:metabolism; D018149:Glucose Intolerance / Q000209:etiology / Q000378:metabolism; D006706:Homeostasis; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D015415:Biomarkers; D004041:Dietary Fats; D008054:Lipid Peroxides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D005632:Fructose; C024989:coenzyme Q10; D005947:Glucose