Rapid identification of Salmonella enterica subsp. arizonae and S. enterica subsp. diarizonae by real-time polymerase chain reaction.
Reptiles are popular as pets, leading to an increased risk of human infections due to uncommon Salmonella strains including the Arizona group (subspecies arizonae and diarizonae). We present a real-time Arizona-specific polymerase chain reaction demonstrating 100% specificity and 99.6% sensitivity, offering savings in time and labor over traditional identification methods.
Hopkins
Katie L
KL
Department of Gastrointestinal, Emerging and Zoonotic Infections, Health Protection Agency Centre for Infections, NW9 5EQ London, United Kingdom. katie.hopkins@hpa.org.uk
Peters
Tansy M
TM
Lawson
Andy J
AJ
Owen
Robert J
RJ
D000818:Animals; D001130:Arizona; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012104:Reptiles / Q000382:microbiology; D012480:Salmonella Infections / Q000175:diagnosis* / Q000382:microbiology*; D012476:Salmonella arizonae / Q000145:classification / Q000302:isolation & purification; D019779:Salmonella enterica / Q000145:classification* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
D004269:DNA, Bacterial