title: Antiretroviral therapy initiation during primary HIV infection enhances both CD127 expression and the proliferative capacity of HIV-specific CD8+ T cells.
abstract: OBJECTIVE
  HIV-specific CD8+ T cells from patients with primary HIV infection (PHI) and after antiretroviral therapy initiation were evaluated for CD127 expression and proliferative capacity and were compared with cells from chronically-infected patients, including long-term nonprogressors and HIV controllers.
  
  
  METHODS
  We studied 30 patients with PHI (from the Agence Nationale de Recherche sur le SIDA Primo-infection Cohort) and 33 patients with chronic HIV infection (including nonprogressor patients from the Agence Nationale de Recherche sur le SIDA ALT Cohort and the Agence Nationale de Recherche sur le SIDA HIV Controllers Study Group). HIV-specific CD8+ T cells were identified by costaining with HIV human leukocyte antigen class I pentamers. CD127 expression was assessed by flow cytometry and cell proliferation by carboxyfluorescein succinimidyl ester labeling.
  
  
  RESULTS
  During PHI, most HIV-specific CD8+ T cells coexpressed CD27 and CD45RO, were highly activated, and showed weak Bcl-2 expression. Their CD127 expression was very low and correlated negatively both with HIV RNA and DNA levels and with expression of the activation marker CD38. CD127 expression correlated positively with CD4 cell count, Bcl-2 expression and proliferative capacity. Strong CD127 expression was observed in the two groups of chronically-infected nonprogressors. CD127 expression on HIV-specific CD8+ T cells increased in early-treated PHI patients, reaching levels similar to those observed in nonprogressors. In parallel, these cells acquired strong proliferative capacity. No change in CD127 expression or proliferative potential was observed in untreated patients.
  
  
  CONCLUSIONS
  Early antiretroviral therapy initiation enhances CD127 expression on HIV-specific CD8+ T cells, reaching levels similar to those observed in aviremic nonprogressors, and restores their proliferative capacity.
authors.affiliation: INSERM U 802, Université Paris-Sud XI, AP-HP Hôpital Bicêtre, Le Kremlin Bicêtre, France. camille.lecuroux@u-psud.fr
authors.forename: Camille
authors.initials: C
authors.lastname: Lécuroux
authors.forename: Isabelle
authors.initials: I
authors.lastname: Girault
authors.forename: François
authors.initials: F
authors.lastname: Boutboul
authors.forename: Alejandra
authors.initials: A
authors.lastname: Urrutia
authors.forename: Cécile
authors.initials: C
authors.lastname: Goujard
authors.forename: Laurence
authors.initials: L
authors.lastname: Meyer
authors.forename: Olivier
authors.initials: O
authors.lastname: Lambotte
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Chaix
authors.forename: Valérie
authors.initials: V
authors.lastname: Martinez
authors.forename: Brigitte
authors.initials: B
authors.lastname: Autran
authors.forename: Martine
authors.initials: M
authors.lastname: Sinet
authors.forename: Alain
authors.initials: A
authors.lastname: Venet
mesh_terms: D000208:Acute Disease; D019380:Anti-HIV Agents / Q000627:therapeutic use*; D018791:CD4 Lymphocyte Count; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002908:Chronic Disease; D018450:Disease Progression; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology / Q000821:virology; D020276:HIV Long-Term Survivors; D006801:Humans; D016130:Immunophenotyping; D053699:Interleukin-7 Receptor alpha Subunit / Q000097:blood*; D008213:Lymphocyte Activation / Q000276:immunology; D019562:Viral Load; D014766:Viremia / Q000276:immunology
chemical_list: D019380:Anti-HIV Agents; D053699:Interleukin-7 Receptor alpha Subunit