Sequence specific primer extension RNA analysis (SeSPERA) for the investigation of substrate utilization of microbial communities.
A simple, fast and cost-effective method for the taxon-specific separation of various 16S rRNAs was developed. This primer extension mediated separation of different RNA species was obtained on agarose gels. The method provides an easy way for the investigation of substrate mediated radioisotope incorporation into the RNA.
Franchini
Alessandro G
AG
Department of Environmental Biotechnology, UFZ, Helmholtz Centre for Environmental Research, Leipzig, Germany.
Nikolausz
Marcell
M
Kästner
Matthias
M
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D017931:DNA Primers / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011868:Radioisotopes / Q000378:metabolism*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D011868:Radioisotopes