Hirschia maritima sp. nov., isolated from seawater.
A Gram-negative, aerobic, orange-coloured bacterium, designated strain GSW-2T, was isolated from seawater sampled at a beach and its taxonomic status was established by using a polyphasic approach. Vegetative cells produced a prostheca and reproduced by budding. Mature vegetative cells were spherical, oval- or rod-shaped (0.6-1.1x1.2-1.3 microm) and both vegetative cells and buds produced one or more flagella. Cells grew well at 30 degrees C and at pH 8.1-9.1, and produced a non-diffusible pigment. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSW-2T was most closely related to the type strain of Hirschia baltica (97.5% similarity), a member of the family Hyphomonadaceae, class Alphaproteobacteria. Chemotaxonomic characteristics supported the assignment of strain GSW-2T to the genus Hirschia, including the dominant cellular fatty acids (summed feature 7 and C16:0), isoprenoid quinone (mainly Q-10) and DNA G+C content (44.5 mol%). Levels of DNA-DNA relatedness between strain GSW-2T and Hirschia baltica DSM 5838T were 8.8-13.6%. On the basis of phenotypic features and DNA-DNA hybridization data, strain GSW-2T is considered to represent a novel species of the genus Hirschia, for which the name Hirschia maritima sp. nov. is proposed. The type strain is GSW-2T (=DSM 19733T=JCM 14974T).
Kang
Hye Soon
HS
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Lee
Soon Dong
SD
D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S