[The role of CXCR1/2 in shear stress-induced endothelial cell migration].
CXCR1 and CXCR2 are important receptors in regulating vascular endothelial cell activities. In order to elucidate the role of CXCR1/2 in shear stress-induced endothelial cell migration, we have investigated the expression levels of CXCR1 and CXCR2 in the endothelial cells exposed to shear stress. In the experiment, anti-IL8RA and anti-IL8RB were used to antagonize CXCR1 and CXCR2. Different shear stresses were generated in a flow chamber; scratch test was carried out to compare endothelial cell migration in the control group and the receptor-antagonized groups. The results indicated that the migration of endothelial cells was restrained effectively after CXCR1 and CXCR2 were antagonized by anti-IL8RA and anti-IL8RB. And anti-IL8RA showed a stronger inhibitive effect than did anti-IL8RB (P<0.05). In the group with both receptor antagonisms, the migration was further inhibited. These results suggest that both CXCR1 and CXCR2 are important factors in mediating the migration of endothelial cells induced by shear stress, and CXCR1 fulfills a more important role.
Sun
Hurong
H
Institute of Biomedical Engineering, West China College of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wei
Lin
L
Liu
Xiaoheng
X
Zeng
Ye
Y
Lai
Yi
Y
Yin
Hongmei
H
D002465:Cell Movement / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000187:drug effects*; D023062:Receptors, Interleukin-8A / Q000037:antagonists & inhibitors / Q000502:physiology*; D023063:Receptors, Interleukin-8B / Q000037:antagonists & inhibitors / Q000502:physiology*; D033081:Shear Strength; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B