Inflammatory cytokines decrease the expression of nicotinic acetylcholine receptor during the cell maturation.
It is known that the nervous system significantly attenuates systemic inflammatory responses through the parasympathetic nervous system. Furthermore, it has been reported that the alpha 7 subunit of a nicotinic acetylcholine receptor is required for a cholinergic inhibition against cytokine synthesis in a macrophage. As antigen-presenting cells (APCs) play a central role in the generation of primary T cell responses and the maintenance of immunity, in this study, we investigated the expression level of nicotinic receptors of a p53-deficient APC cell line (JawsII) derived from a mouse bone marrow. We showed that stimulation of the JawsII cells with lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNF-alpha) led increase of CD80 and CD86 expression while diminishment of the surface nicotinic receptor. On the other hand, stimulation of nicotinic receptor had no effect on these phenomena. Furthermore, we examined the ability of the cells to release cytokine when stimulated with both nicotine and LPS and showed that the stimulation with LPS augmented the secretion of IL-1a, IL-1b, IL-6, and TNF-alpha. These results suggested that nicotinic stimulation had no effect on the diminishment of alpha 7 nicotinic acetylcholine receptor on JawsII cells by LPS stimulation.
Kondo
Yukiko
Y
Department of Pharmacology, Iwate Medical School, Bldg 3-4, 19-1 Uchimaru, Morioka, Iwate, 020-8505, Japan.
Tachikawa
Eiichi
E
Ohtake
Shinpei
S
Kudo
Kenzo
K
Mizuma
Kenzo
K
Kashimoto
Takeshi
T
Irie
Yasuyuki
Y
Taira
Eiichi
E
D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D018836:Inflammation Mediators; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009538:Nicotine / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000235:genetics*
D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D011978:Receptors, Nicotinic; D009538:Nicotine