title: Simultaneous analysis of liposomal doxorubicin and doxorubicin using capillary electrophoresis and laser induced fluorescence.
abstract: A method based on a capillary electrophoresis with laser induced fluorescence detection was developed and validated for simultaneous separation of doxorubicin (DOX) and liposomal encapsulated DOX. The separation was accomplished using a fused silica capillary (60cm in total length, 75microm I.D.) and potassium phosphate buffer [12.5mM, pH 7.4] as the running buffer. The effect of sample preparation conditions on maintaining liposomal integrity was also investigated. The limit of detection for DOX was 0.1microg/ml and the precision and accuracy of CE/LIF method was within the ranges of FDA guidelines. The validated method was successfully used to quantify DOX in human plasma using a direct injection of a 4-fold dilution of spiked liposomal DOX in human plasma.
authors.affiliation: Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. hskjay@hotmail.com
authors.forename: Hee Seung
authors.initials: HS
authors.lastname: Kim
authors.forename: Irving W
authors.initials: IW
authors.lastname: Wainer
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D004317:Doxorubicin / Q000032:analysis*; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D005615:Freezing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D057230:Limit of Detection; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D002021:Buffers; D004317:Doxorubicin