[Molecular analysis of soluble methane monooxygenase and 16S rDNA from a type II methanotroph].
The soluble methane monooxygenase (sMMO) from Methylosinus trichosporium IMV 3011 catalyzes the conversion of methane to methanol.


OBJECTIVE
To identify the novel species Methylosinus trichosporium IMV 3011 and to describe its evolution status.


METHODS
With the aid of the information from GenBank, we designed several sets of primers for PCR amplification and sequencing, the 16S rDNA and complete of genes sequence for soluble methane monooxygenases were gene sequenced and analyzed with biology software.


RESULTS
We obtained a 5319 bp of full-length DNA of soluble methane monooxygenases and a 1290 bp of 16S rDNA. Software analysis for six open reading frames and the deduced amino acid sequences of soluble methane monooxygenases has shown that 99.0% to 82.7% identity to the counterpart of Methylosinus trichosporium OB3b, 99.4% to 81.8% identity and 99.8% to 89.2% similarity to the predicted amino acid of mmoX genes in compared five strains. The multiple alignments of MMOX amino acid residues reveal that there is high conservation in MMOX, especially in two Fe binding regions.


CONCLUSIONS
These results indicated that strain IMV 3011 should be a true member of Methylosinus trichosporium, and it is closer to the species Methylosinus trichosporium OB3b.
Hua
Shaofeng
S
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. sfhua@lzb.ac.cn
Li
Shuben
S
D000595:Amino Acid Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D020602:Methylosinus trichosporium / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D004275:DNA, Ribosomal; D010105:Oxygenases; C021555:methane monooxygenase