title: Gene expressions of HMGI-C and HMGI(Y) are associated with stage and metastasis in colorectal cancer.
abstract: OBJECTIVE
  The high mobility group proteins (HMGs) include the HMGI family members HMGI-C and HMGI(Y), whose expressions in adult tissues generally correlate with malignant tumor phenotypes. The aim of this study was to assess the relationship of HMGI-C or HMGI(Y) gene expression and prognosis in colorectal cancer patients.
  
  
  METHODS
  The gene expressions of HMGI-C and HMGI(Y) in 31 paired samples of colorectal tumor and corresponding non-tumor were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The expression of HMGI(Y) in a colorectal cancer tumor was associated with Dukes staging (p = 0.044), while, in non-tumor, the expression of this gene was significant with metastasis (p = 0.003). Patients with Dukes stage A and B present high HMGI(Y) expression in non-tumor of colorectal cancer (p = 0.006). However, patients with Dukes stage C and D present high HMGI-C expression in colorectal tumor (p = 0.023). In the non-metastasis group, HMGI(Y) was highly expressed in non-tumor of colorectal cancer. However, in the metastasis group, there was no significant difference between tumor and non-tumor tissues in both HMGI-C and HMGI(Y) gene expressions.
  
  
  CONCLUSIONS
  The HMGI-C and HMGI(Y) quantitations by real-time RT-PCR are associated with Dukes staging and metastasis; hence, the gene expression levels may be useful in clinical practice.
authors.affiliation: Division of Proctology, Department of Surgery, ZuoYing Armed Forces General Hospital, Kaohsiung, Taiwan, Republic of China.
authors.forename: Meng-Lin
authors.initials: ML
authors.lastname: Huang
authors.forename: Chou-Chan
authors.initials: CC
authors.lastname: Chen
authors.forename: Li-Ching
authors.initials: LC
authors.lastname: Chang
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D025741:HMGA1a Protein / Q000235:genetics* / Q000378:metabolism; D025743:HMGA2 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging
chemical_list: D025743:HMGA2 Protein; D025741:HMGA1a Protein