title: Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages.
abstract: OBJECTIVE
  Pre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.
  
  
  METHODS
  Sorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.
  
  
  RESULTS
  Transcript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.
  
  
  CONCLUSIONS
  Several genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.
authors.affiliation: IFEVA (CONICET-UBA) Facultad de Agronomía, C1417DSE, Buenos Aires, Argentina. mvr@agro.uba.ar
authors.forename: María Verónica
authors.initials: MV
authors.lastname: Rodríguez
authors.forename: Guillermina Mónica
authors.initials: GM
authors.lastname: Mendiondo
authors.forename: Laura
authors.initials: L
authors.lastname: Maskin
authors.forename: Gustavo Eduardo
authors.initials: GE
authors.lastname: Gudesblat
authors.forename: Norberto Daniel
authors.initials: ND
authors.lastname: Iusem
authors.forename: Roberto Luis
authors.initials: RL
authors.lastname: Benech-Arnold
mesh_terms: D000040:Abscisic Acid / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics; D018525:Germination / Q000235:genetics* / Q000502:physiology; D010937:Plant Growth Regulators / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D045868:Sorghum / Q000235:genetics* / Q000254:growth & development
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid