title: [Determination of contents of tripterine in Tripterygii preparations].
abstract: OBJECTIVE
  To establish the HPLC method of determining the contents of tripterine in Tripterygii preparations purchased from drugstores.
  
  
  METHODS
  An external standard method by HPLC with Hanbon Kromasil C18 column as fixed phase and methanol-1% HAc (83:17) as mobile phase was adopted. The detection wavelength was 425 nm and the flow rate was 1.0 mL x min(-1).
  
  
  RESULTS
  The contents of tripterine in TWH F. tablets were between 89.04 and 344.0 microg/tablet, and is higher than those in TWH F. polyglycosidium (14.98-217.80 microg/tablet) and Kunmingshanhaitang tablets (22.32 microg/tablet).
  
  
  CONCLUSIONS
  The contents of tripterine, in different Tripterygium preparations vary significantly, and in Tripterygium preparations from different manufacturers and different production lots are being remarkable discrepancy. So it is necessary to enhance quality monitoring and to establish the rational quality control standard for Tripterygii preparations.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Wenhua
authors.initials: W
authors.lastname: Huang
authors.forename: Baolin
authors.initials: B
authors.lastname: Guo
authors.forename: Jinping
authors.initials: J
authors.lastname: Si
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D016014:Linear Models; D053978:Pentacyclic Triterpenes; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D029757:Tripterygium / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C050414:celastrol