title: Impact of a rinse step on protein removal from silicone hydrogel contact lenses.
abstract: OBJECTIVE
  To determine the impact of the rinse step in "no rub" contact lens care systems relative to its ability to assist in removing loosely associated and bound tear film proteins from a worn silicone hydrogel lens.
  
  
  METHODS
  After informed consent, subjects were fitted with lotrafilcon B contact lenses (CIBA Vision). If the fit was acceptable, subjects were asked to wear the lenses on a daily wear basis for 5 (+2, -0) days for an outcome visit. Subjects were instructed to use AQuify Multi-Purpose Disinfecting Solution (CIBA Vision) following the manufacturer's "no rub" instructions. At the outcome visit, contact lenses were then collected by a gloved examiner, with a sterile metal forceps, who rinsed the right lens but did not rinse the left lens on removal from the eyes. Protein was extracted with a 50:50 0.2% trifluoroacetic acid-acetonitrile solution and quantified using a Bradford analyses.
  
  
  RESULTS
  Twenty contact lens wearers were enrolled in this study. For the non-rinsed lenses, the first extraction yielded 13.4 +/- 9.2 microg/lens of protein, whereas the second extraction yielded 5.8 +/- 2.8 microg/lens of protein. For the rinsed lenses, the first extraction yielded an average of 3.0 +/- 1.9 microg/lens of protein, whereas the second extraction yielded an average of 4.0 +/- 2.3 microg/lens. Repeated measures analysis of variance showed a significant interaction (F-statistic = 18.9, p < 0.0001) between the rinse of a lens and extraction number.
  
  
  CONCLUSIONS
  Rinsing a contact lens after removal from the eye removes well more than one-half of the protein associated with it. Further, to biochemically recover all protein from a silicone hydrogel lens, it may be important to perform more than one chemical extraction from it.
authors.affiliation: The Ohio State University College of Optometry, Columbus, Ohio 43218-2342, USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Pucker
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Nichols
mesh_terms: D000328:Adult; D018639:Contact Lens Solutions; D003263:Contact Lenses, Hydrophilic; D005136:Eye Proteins; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels; D006920:Hygiene / Q000592:standards; D008297:Male; D011446:Prospective Studies; D012828:Silicones; D014867:Water; D055815:Young Adult
chemical_list: D018639:Contact Lens Solutions; D005136:Eye Proteins; D020100:Hydrogels; D012828:Silicones; C524582:lotrafilcon B; C042434:tear proteins; D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate