[Purification and characterization of an antimicrobial peptide from Paris polyphylla var. chinensis].
OBJECTIVE
We isolated an endophyte PCE45 from the rhizome of Paris polyphylla var. chinensis. From PCE45, we purified and characterized an antimicrobial peptide.


METHODS
After ammonium sulfate salting-out, acetone precipitation, SephadexG75, DE52 and SephadexG25 column chromatography, we separated an antimicrobial peptide PCP-1 from the strain PCE45. The stability against high temperature and proteinase, and antimicrobial activity were also analyzed.


RESULTS
The antimicrobial peptide PCP-1 was stable to proteinase and tolerated high temperature, strong acid and strong base. PCP-1 caused deformation of the hyphae of Pyrictlaria oryzae and prohibited the spore germination. It also inhibited fungi such as Curvularia lunata and bacteria such as Escherichia colli. Mass spectrogram measurement revealed its molecular weight of 1058.3 Da. The amino acid composition of the peptide composed of 7 amino acids. Ninhydrin reaction showed negative trait whereas after acid hydrolysis with positive ninhydrin reaction and biuret reaction.


CONCLUSIONS
The ninhydrin reaction and biuret reaction imply that the peptide PCP-1 is a cyclic lipeptide. This is the first report about antimicrobial peptide from Paris polyphylla var. chinensis.
Cheng
Yuanyuan
Y
College of Life Science, Sichuan Normal University, Chengdu 610068, China. chengyuanyuan910@163.com
Yong
Bin
B
Zhang
Chao
C
Liu
Qiang
Q
Yan
Wei
W
Wang
Yiding
Y
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects; D025301:Hyphae / Q000187:drug effects; D020082:Magnaporthe / Q000187:drug effects; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*; D013172:Spores, Fungal / Q000187:drug effects
D000890:Anti-Infective Agents; D010456:Peptides, Cyclic