Absence of Simian virus 40, BK, and JC polyomavirus DNA in squamous cell carcinoma limited to the oral cavity.
BACKGROUND
Head and neck squamous cell carcinomas (SCCs) are among the most aggressive types of cancer. The Simian virus 40 (SV40), which is a polyomavirus known for its oncogenic potential, was found as a contaminant of oral vaccines and has been related to human pleomorphic adenoma in the parotid gland. The aim of this study was to evaluate the presence of SV40 and 2 human polyomaviruses-BK virus (BKV) and JC virus (JCV)-in a large sample of SCCs of the oral cavity.


METHODS
Quantitative real-time polymerase chain reaction (PCR) was used to evaluate virus load.


RESULTS
Overall, the prevalence of SV40, BKV, and JCV in oral SCC was negligible. Matched-pair case-control analysis indicated that prevalence among the controls did not significantly differ with respect to analyzed cases.


CONCLUSIONS
The results did not indicate a major role for SV40, BKV, and JCV in the etiology of oral SCC.
Palmieri
Annalisa
A
Department of Histology, Embryology and Applied Biology, Centre of Molecular Genetics, University of Bologna, Bologna, Italy.
Carinci
Francesco
F
Martinelli
Marcella
M
Spinelli
Giuseppe
G
Lo Muzio
Lorenzo
L
Rubini
Corrado
C
Scapoli
Luca
L
D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D016022:Case-Control Studies; D015331:Cohort Studies; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology / Q000821:virology*; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019562:Viral Load
D004269:DNA, Bacterial