title: [The first report of Kadipiro virus isolation in China].
abstract: 5 strains of virus isolated from Culex tritaeniorhynchus, Anopheles sinensis and Armigeres subalbatus, which caused cytopathic effect in C6/36 cells, had been obtained in the survey of arboviruses in Northwestern Yunnan Province. China. The virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. RNA-PAGE of the genomes of the isolates showed 6-5-1 profile. A fragment of the 12th segment sequence was amplified by a pair of specific primers for Kadipiro virus strain JKT-7075 in RT-PCR. The full length of the 12th segment was 758 nucleotides, BLAST analysis revealed the highest identity was 90% to JKT-7075. Phylogenetic analysis demonstrated that the isolates appeared to be Kadipiro viruses (Family Reoviridae). It was the first report of kadipiro virus isolation in China.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. dotsunny@hotmail.com
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Wei-shan
authors.initials: WS
authors.lastname: Meng
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: You-gang
authors.initials: YG
authors.lastname: Zhai
authors.forename: Jing-lin
authors.initials: JL
authors.lastname: Wang
authors.forename: Huan-qin
authors.initials: HQ
authors.lastname: Wang
authors.forename: Xin-jun
authors.initials: XJ
authors.lastname: Lv
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000821:virology; D002460:Cell Line; D002681:China; D018162:Coltivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003465:Culex / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA