Dietary protein intake and stage of lactation differentially modulate amino acid transporter mRNA abundance in porcine mammary tissue.
To test the hypothesis that under restricted and surfeit protein intake the mammary gland undergoes adaptive regulation, changes in mammary tissue mRNA abundance of cationic amino acid (AA) transporter (CAT)-1, CAT-2B, alanine/serine/cysteine/threonine transporter 1 (ASCT1), and broad specificity transporter for neutral and cationic AA (ATB(0,+)), and CAT-1 protein abundance were investigated at 2 stages of lactation. Eighteen sows were allocated to a 2 x 3 randomized incomplete block design with 2 stages of lactation (early and peak) and 3 protein levels: deficient (D), adequate (A), or in excess (E) of lactation requirement. In early lactation, compared with A, sows fed E had lower (P = 0.05) piglet growth rate and sows fed D or E had lower (P < or = 0.05) casein yield. In early lactation, piglet growth rate and milk protein and casein yield increased from D to A and decreased from A to E (quadratic, P = 0.095, P < 0.05, and P < 0.01, respectively). Protein intake did not affect CAT-1, ASCT1, ATB(0,+) mRNA abundance, or CAT-1 protein level. Overall, CAT-2B mRNA abundance decreased linearly with increasing protein intake (P < 0.05). Compared with A, E decreased CAT-2B mRNA abundance (P < 0.05). Compared with early lactation, peak lactation did not increase CAT-1 mRNA abundance or relative CAT-1 protein content, but increased abundance of ASCT1 and ATB(0,+) mRNA (P < 0.01). Mammary CAT-2B appears to be adaptively regulated in vivo at the transcription level, whereas ASCT1 and ATB(0,+) mRNA abundances are associated only with stage of lactation. Neither protein intake nor stage of lactation affects porcine mammary CAT-1 gene expression in vivo.
Laspiur
Juliana Pérez
JP
Department of Animal Science and 5Department of Large Animal Clinical Science, Michigan State University, East Lansing, MI 48824, USA.
Burton
Jeanne L
JL
Weber
Patty S D
PS
Moore
Julie
J
Kirkwood
Roy N
RN
Trottier
Nathalie L
NL
D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D002364:Caseins / Q000378:metabolism; D004032:Diet; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D011488:Protein Deficiency / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D013552:Swine; D015430:Weight Gain
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D002364:Caseins; D004044:Dietary Proteins; D012333:RNA, Messenger