Anchored and soluble gangliosides contribute to myelosupportivity of stromal cells.
Stroma-mediated myelopoiesis depends upon growth factors and an appropriate intercellular microenvironment. Previous studies have demonstrated that gangliosides, produced by hepatic stromal cell types, are required for optimal myelosupportive function. Here, we compared the mielossuportive functions of a bone marrow stroma (S17) and skin fibroblasts (SF) regarding their ganglioside pattern of synthesis and shedding. The survival and proliferation of a myeloid precursor cell (FDC-P1) were used as reporter. Although the ganglioside synthesis of the two stromal cells was similar, their relative content and shedding were distinct. The ganglioside requirement for mielossuportive function was confirmed by the decreased proliferation of FDC-P1 cells in ganglioside synthesis-inhibited cultures and in presence of an antibody to GM3 ganglioside. The distinct mielossuportive activities of the S17 and SF stromata may be related to differences on plasma membrane ganglioside concentrations or to differences on the gangliosides shed and their subsequent uptake by myeloid cells, specially, GM3 ganglioside.
Ziulkoski
Ana L
AL
ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
dos Santos
Aline X S
AX
Andrade
Cláudia M B
CM
Trindade
Vera M T
VM
Daniotti
José Luis
JL
Borojevic
Radovan
R
Guma
Fátima C R
FC
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D049109:Cell Proliferation; D005347:Fibroblasts / Q000502:physiology; D005679:G(M3) Ganglioside / Q000494:pharmacology / Q000502:physiology; D005732:Gangliosides / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D038042:Myelopoiesis; D012867:Skin / Q000166:cytology; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology
D005679:G(M3) Ganglioside; D005732:Gangliosides