Influence of reduction in the elution times on HPLC glycohaemoglobin results.
OBJECTIVE
To compare HPLC methods with short and long elution times for HbA1c measurement in blood.


METHODS
Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min).


RESULTS
Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means of the differences using Bland-Altman Plot were 0.02; -0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively.


CONCLUSIONS
Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.
Leon-Justel
Antonio
A
Department of Clinical Biochemistry, Laboratory Buildings (4th Floor), Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville, Seville, Spain. antonio.leonj.sspa@juntadeandalucia.es
Santotoribio
Jose D
JD
Dominguez-Pascual
Inmaculada
I
Delgado
Ana L
AL
Macias
Carlos
C
Herrera
Maria T
MT
Leal
Alfonso
A
Mangas
Miguel A
MA
Acosta
Domingo
D
Guerrero
J M
JM
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005319:Fetal Hemoglobin / Q000032:analysis; D006442:Glycated Hemoglobin / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis; D006801:Humans; D013997:Time Factors
D005319:Fetal Hemoglobin; D006442:Glycated Hemoglobin; D006451:Hemoglobin, Sickle; C517652:hemoglobin A1c protein, human