Affinity study of novel gelatin cell carriers for fibronectin.
In the present work, the gelatin/fibronectin affinity was evaluated using SPR, QCM and radiolabelling. The results indicate that type A gelatin films possess a higher affinity for Fn compared to type B gelatin. This is due to a combined hydrophobic and electrostatic interaction between gelatin type A and Fn. In a second part, the affinity of Fn for porous gelatin scaffolds was evaluated. The scaffolds were prepared by a cryogenic treatment and subsequent freeze-drying yielding type I and type II scaffolds which possess different pore geometries/sizes. The results indicate that the Fn density on the scaffolds can be fine-tuned by varying the Fn concentration, the gelatin type (A vs. B), the pore size/geometry (type I vs. type II scaffolds).
Van Vlierberghe
Sandra
S
Polymer Chemistry and Biomaterials Research Group, Ghent University, Ghent, Belgium.
Vanderleyden
Els
E
Dubruel
Peter
P
De Vos
Filip
F
Schacht
Etienne
E
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
D001672:Biocompatible Materials; D005353:Fibronectins; D020100:Hydrogels; C015233:gelatin film; D005780:Gelatin