title: LIF-free embryonic stem cell culture in simulated microgravity.
abstract: BACKGROUND
  Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.
  
  
  RESULTS
  We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.
  
  
  CONCLUSIONS
  Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
authors.affiliation: Department of Clinical Neuroscience and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kawahara
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Manabe
authors.forename: Masaya
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Kajiume
authors.forename: Masayasu
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Louis
authors.initials: L
authors.lastname: Yuge
mesh_terms: D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D017931:DNA Primers; D053595:Embryonic Stem Cells / Q000166:cytology*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D014893:Weightlessness
chemical_list: D017931:DNA Primers; D053605:Leukemia Inhibitory Factor