Isolation of lactobacilli from sow milk and evaluation of their probiotic potential.
Sow milk protects the piglet against infectious diseases through a variety of mechanisms. In this study, the presence of potentially probiotic lactic acid bacteria in this biological fluid was investigated. Milk samples were obtained from 8 sows and a total of 19 rod-shaped isolates were selected for identification and assessment of their probiotic potential. RAPD profiling revealed the existence of 8 different genetic profiles among them. One representative of each profile was selected for further characterization and they were identified as Lactobacillus reuteri, Lb. salivarius, Lb. plantarum, Lb. paraplantarum, Lb. brevis and Weissella paramesenteroides. Then, their probiotic potential was evaluated through different assays, including survival in conditions simulating those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, production of biogenic amines, degradation of mucin, and pattern of antibiotic sensitivity. Three strains, Lb. reuteri CR20 (a reuterin-producing strain), Lb. salivarius CELA2 (a bacteriocin-producing strain) and Lb. paraplantarum CLB7 displayed the highest probiotic potential.
Martín
Rocío
R
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Delgado
Susana
S
Maldonado
Antonio
A
Jiménez
Esther
E
Olivares
Mónica
M
Fernández
Leónides
L
Sobrino
Odón J
OJ
Rodríguez
Juan M
JM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D005260:Female; D007778:Lactobacillus / Q000187:drug effects / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D019936:Probiotics; D013552:Swine / Q000382:microbiology*
D000900:Anti-Bacterial Agents