Transplanted XY germ cells produce spermatozoa in testes of XXY mice.
XXY mouse has been characterized as an experimental model for men with Klinefelter's syndrome (XXY male phenotype). To test whether donor XY germ cells could proliferate and differentiate in the XXY testicular environment, donor testicular cells from adult (2-3 months old) and immature (10 days old) XY green fluorescence protein (GFP) transgenic mice were transplanted into the seminiferous tubules of adult (4-7 months old) and young (6 weeks old) XXY recipient mice respectively. Twelve weeks after transplantation, GFP positive spermatogonia were found in 21.74% (five out of 23) of adult XXY recipients who received adult donor cells. The GFP positive segments of seminiferous tubules were observed in 44.44% (four out of nine) young XXY recipients who received donor cells from 10 days old GFP mice. We found using immunohistochemistry and cell morphology that donor-derived GFP positive germ cells were spermatogonia, spermatocytes, round spermatids and spermatozoa in some of the seminiferous tubules of young XXY recipient mice. The results demonstrated that the donor XY germ cells were able to qualitatively complete spermatogenesis in some of the seminiferous tubules of XXY mice.
Lue
Y
Y
Division of Endocrinology, Department of Medicine, David Geffen School of Medicine at UCLA, Harbor-UCLA Medical Center, Torrance, CA 90509, USA.
Liu
P Y
PY
Erkkila
K
K
Ma
K
K
Schwarcz
M
M
Wang
C
C
Swerdloff
R S
RS
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005854:Germ Cells / Q000637:transplantation*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007713:Klinefelter Syndrome / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013094:Spermatozoa / Q000166:cytology; D013737:Testis / Q000166:cytology*
D049452:Green Fluorescent Proteins