Marinococcus luteus sp. nov., a halotolerant bacterium isolated from a salt lake, and emended description of the genus Marinococcus.
A Gram-positive, aerobic, motile, coccoid, orange-pigmented bacterium, designated strain YIM 91094(T), was isolated from a salt lake sample collected from Barkol Lake in Xinjiang Province, north-west China. The strain was able to grow at pH 6.0-8.0 (optimal growth at pH 7.0), at 10-37 degrees C (optimal growth at 28 degrees C) and in the presence of 0-25 % (w/v) NaCl [optimal growth in the presence of 10-15 % (w/v) NaCl]. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 91094(T) was affiliated with the genus Marinococcus and exhibited levels of sequence similarity of 99.2 % to Marinococcus halotolerans YIM 70157(T) and 99.7 % to Marinococcus halophilus DSM 20408(T). However, it showed moderately low levels of DNA-DNA relatedness with the above type strains (56.0 and 57.5 %, respectively). The peptidoglycan type of strain YIM 91094(T) was A1gamma, with meso-diaminopimelic acid as the diagnostic diamino acid. MK-7 was the predominant menaquinone and anteiso-C(15 : 0) (49.9 % of the total) and anteiso-C(17 : 0) (29.6 %) were the major cellular fatty acids. The DNA G+C content was 48.7 mol%. Strain YIM 91094(T) possessed chemotaxonomic markers that were consistent with its classification in the genus Marinococcus. On the basis of the data presented, strain YIM 91094(T) is considered to represent a novel species of the genus Marinococcus, for which the name Marinococcus luteus sp. nov. is proposed. The type strain is YIM 91094(T) (=KCTC 13214(T)=CCTCC AA 208014(T)). An emended description of the genus Marinococcus is provided.
Wang
Yun
Y
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang 830091, People's Republic of China.
Cao
Lan-Lan
LL
Tang
Shu-Kun
SK
Lou
Kai
K
Mao
Pei-Hong
PH
Jin
Xiang
X
Jiang
Cheng-Lin
CL
Xu
Li-Hua
LH
Li
Wen-Jun
WJ
D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000378:metabolism; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride