Sequence-specific 1H, 13C, and 15N backbone assignment of the GTPase rRheb in its GDP-bound form.
Rheb (Ras homologue enriched in brain) is a small GTPase that plays an important role in tuberous sclerosis. Here we present the backbone assignments of rRheb in its GDP-bound state. These assignments now provide a basis for the analysis of the interaction of rRheb with putative factors in order to elucidate the function of this GTPase and its role in the MAP kinase pathway of neuronal cells and in tuberous sclerosis.
Berghaus
Carsten
C
Biomolecular NMR, Faculty of Chemistry and Biochemistry, Ruhr-University of Bochum, Bochum 44780, Germany.
Schwarten
Melanie
M
Heumann
Rolf
R
Stoll
Raphael
R
D006153:Guanosine Diphosphate / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D020935:MAP Kinase Signaling System; D015394:Molecular Structure; D020559:Monomeric GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D000076205:Ras Homolog Enriched in Brain Protein; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014402:Tuberous Sclerosis / Q000201:enzymology / Q000235:genetics
D009479:Neuropeptides; C490211:RHEB protein, human; D000076205:Ras Homolog Enriched in Brain Protein; D011994:Recombinant Proteins; D006153:Guanosine Diphosphate; D020559:Monomeric GTP-Binding Proteins