Sustained and extended release with structural and activity recovery of lysozyme from complexes with sodium (sulfamate carboxylate) isoprene/ethylene oxide block copolymer.
The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Through electrostatic and hydrophobic interactions, lysozyme and SCIEO form stable complex nanoparticles. The complexation partially disturbs the structure of lysozyme. Some of the hydrophobic residues of lysozyme are exposed to bind with SCIEO. The complexation leads to loss of most of the lysozyme activity. In the presence of NaCl, lysozyme can be released from the complexes. The released lysozyme molecules recover their native structure and activity completely. In the condition of physiological pH and ionic strength, a sustained and extended release of lysozyme was achieved.
Gao
Gao
G
Key Laboratory of Molecular Engineering of Polymers, and Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
Yan
Yunfeng
Y
Pispas
Stergios
S
Yao
Ping
P
D000818:Animals; D002021:Buffers; D002070:Butadienes / Q000737:chemistry*; D002417:Cattle; D002942:Circular Dichroism; D005027:Ethylene Oxide / Q000737:chemistry*; D005260:Female; D045782:Hemiterpenes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008027:Light; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D009994:Osmolar Concentration; D010420:Pentanes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry*; D013997:Time Factors
D002021:Buffers; D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D013451:Sulfonic Acids; C005059:isoprene; D011092:Polyethylene Glycols; D012965:Sodium Chloride; C421864:hen egg lysozyme; D009113:Muramidase; D005027:Ethylene Oxide