title: Metabolite regulation of the interaction between Arabidopsis thaliana PII and N-acetyl-l-glutamate kinase.
abstract: The metabolic control of the interaction between ArabidopsisN-acetyl-l-glutamate kinase (NAGK) and the PII protein has been studied. Both gel exclusion and affinity chromatography analyses of recombinant, affinity-purified PII (trimeric complex) and NAGK (hexameric complex) showed that NAGK strongly interacted with PII only in the presence of Mg-ATP, and that this process was reversed by 2-oxoglutarate (2-OG). Furthermore, metabolites such as arginine, glutamate, citrate, and oxalacetate also exerted a negative effect on the PII-NAGK complex formation in the presence of Mg-ATP. Using chloroplast protein extracts and PII affinity chromatography, NAGK interacted with PII only in the presence of ATP-Mg(2+), and this process was antagonized by 2-OG. These results reveal a complex metabolic control of the PII interaction with NAGK in the chloroplast stroma of higher plants.
authors.affiliation: Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique, Université Paris Sud, Orsay, France.
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: Feria Bourrellier
authors.forename: Sylvie
authors.initials: S
authors.lastname: Ferrario-Méry
authors.forename: Jean
authors.initials: J
authors.lastname: Vidal
authors.forename: Michael
authors.initials: M
authors.lastname: Hodges
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism*; D051922:PII Nitrogen Regulatory Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007656:Ketoglutaric Acids; D051922:PII Nitrogen Regulatory Proteins; C473074:PII protein, Arabidopsis; D000255:Adenosine Triphosphate; D017851:Phosphotransferases (Carboxyl Group Acceptor); C018721:acetylglutamate kinase; D008274:Magnesium