Mycobacterium tuberculosis secreted antigen (MTSA-10) inhibits macrophage response to lipopolysaccharide by redox regulation of phosphatases.
The present study was undertaken to investigate the possible role of a 10-kDa, secretory antigenic protein of Mtb (MTSA-10) in regulating macrophase response to lipopolysacchride (LPS). MTSA-10 inhibited the lipopolysaccharide (LPS)-induced oxidant species generation in the macrophage. Treatment of macrophages with MTSA-10 activated their protein tyrosine phosphatases (PTPs) in a redox-regulated fashion. These activated phosphatases then interfered with the early events of LPS signaling and lower the strength and magnitude of the signal generated, thereby preventing macrophages from making an effective immune response. Mycobacterium tuberculosis Region of Deletion-1 (RD-1)-specific secretory antigen MTSA-10 (encoded by ORF Rv3874 of Mtb genome) modulated the macrophage signaling machinery and prevented it from responding to further activation by LPS.
Basu
Sandip Kumar
SK
Immunology Group, International Centre for Genetic Engineering and Biotechnology, ICGEB Campus, Aruna Asaf Ali Marg, New Delhi 110 067, India.
Kumar
Dhiraj
D
Ganguly
Niladri
N
Rao
Kanury Venkata Subba
KV
Sharma
Pawan
P
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000276:immunology; D010084:Oxidation-Reduction; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D010744:Phosphoric Monoester Hydrolases