title: Characterization and cytocompatibility of surface modified polyamide66.
abstract: The chemical modification of polyamide66 (PA66) membrane by graft polymerization with methacrylic acid (MAA) was initiated under ultraviolet light. Subsequently, covalent immobilization of bioactive surface was obtained by coupling gelatin to the MAA graft chains with the aid of a water-soluble carbodiimide (WSC). The existence of carboxyl groups grafted on PA66 surface was verified quantitatively by UV-vis spectroscopy. The chemical composition, surface topography, and wettability were investigated by Fourier transform infrared (FTIR) technique, X-ray photoelectron spectroscopy, atomic force microscopy, and water contact angle (WCA) measurement. Results showed that the WCA changed from the original value of 67.5 degrees to the minimum value of 30 degrees after grafting with PMAA. Original PA66 displayed a smooth surface morphology [root mean square (RMS) roughness was around 16 nm]. The modified PA66 surface exhibited an increase in roughness (RMS roughness around 21 nm). Simultaneously, the original and modified PA66 membranes were cultured with MG63 cells to investigate their cytocompatibility. The in vitro biological evaluation demonstrated that the immobilization of gelatin on PA66 membrane acted as a good template for the attachment and proliferation of cells. Also, the less toxic reagents and the moderate reaction conditions involved will be very helpful for the introduction of functional groups onto polymer surface.
authors.affiliation: Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Shen
authors.affiliation: Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Li
authors.affiliation: Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.affiliation: Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008858:Microscopy, Phase-Contrast; D009757:Nylons / Q000737:chemistry*; D010084:Oxidation-Reduction; D010545:Peroxides / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D009757:Nylons; D010545:Peroxides; D005780:Gelatin