1H, 13C and 15N resonance assignment of phage T4 endoribonuclease RegB.
RegB is involved in the control of the phage T4 life cycle. It inactivates the phage early mRNAs when their translation is no more required. We determined its structure and identified residues involved in substrate binding. For this, all backbone and 90% of side-chain resonance frequencies were assigned.
Odaert
Benoît
B
ICSN-RMN, Ecole-Polytechnique (équipe CNRS), Palaiseau, 91128, France.
Saïda
Fakhri
F
Bontems
François
F
D017122:Bacteriophage T4 / Q000201:enzymology* / Q000235:genetics; D020134:Catalytic Domain; D004722:Endoribonucleases / Q000737:chemistry* / Q000235:genetics; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
D011994:Recombinant Proteins; D014764:Viral Proteins; D004722:Endoribonucleases; C407103:T4 RegB endoribonuclease