title: [Research of extracting and purifying taxol from the branches of Taxus media].
abstract: OBJECTIVE
  The study was to explore extracting and purifying technology of taxol from the branches of Taxus media.
  
  
  METHODS
  In extracting phase, the solvent extraction, ultrasonic extraction and the supercritical CO2 fluid extraction were studied respectively; In purifying phase, the extracts were disposed by silica gel column chromatography and preparation lamella chromatography, then crystaled by N-Hexane. The content of taxol was detected by HPLC.
  
  
  RESULTS
  The results showed that the recovery of extracting toxal with the method of ultrasonic was the highest and the selectivity of supercritical CO2 fluid extraction was the best; The sample after being extracted should be purified two stages by silica gel column chromatography with dichloromethane-chloroform-methane (53:44:3) as elution and the lamella chromatography was chloroform-ethyl acetate-methane (88:7:5) as elution, finally we reached the fawn crystal.
  
  
  CONCLUSIONS
  In that production, the content of taxol reached 87. 3% and the recovery 89.7%, which indicat that the taxol is well enrichment.
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Liangjie
authors.initials: L
authors.lastname: Qiao
authors.forename: Ruilin
authors.initials: R
authors.lastname: Man
authors.forename: Wangdong
authors.initials: W
authors.lastname: Ni
authors.forename: Yonghuang
authors.initials: Y
authors.lastname: Liang
mesh_terms: D002725:Chloroform / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D008697:Methane / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D017239:Paclitaxel / Q000737:chemistry* / Q000302:isolation & purification*; D020946:Taxus / Q000737:chemistry*
chemical_list: D008752:Methylene Chloride; D002725:Chloroform; D008697:Methane; D017239:Paclitaxel