title: [Effects of natural plant ginkgolic acids on the apoptosis of human Hep-2 cancer cells].
abstract: OBJECTIVE
  To investigate the anti-cancer effects and mechanism of natural plant ginkgolic acids (GAs) on Human Hep-2 cancer cells.
  
  
  METHODS
  Hep-2 cancer cells were treated with different concentration of GAs for different times. The proliferation of Hep-2 cancer cells were detected by MTT colorimetric assay. Biochemistry characteristics of cell apoptosis was analyzed by DNA agarose electrophoresis and FCM.
  
  
  RESULTS
  The proliferation of Hep-2 cancer cells were inhibited significantly by GAs in a dose and time-dependent manner. Typical DNA ladder bands of Hep-2 cell DNA treated with GAs were observed on agarose electrophoresis gels. Increased apoptosis of Hep-2 cells treated with GAs was also noticed with FCM analysis.
  
  
  CONCLUSIONS
  Ginkgolic acids show an anti-tumor activity through inhibition the growth and inducing apoptosis of tumor cell in vitro.
authors.affiliation: State Key Laboratory of Oral Disease, Sichuan University, Chengdu 610041, China.
authors.forename: Chen-chen
authors.initials: CC
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: Zong
authors.initials: Z
authors.lastname: Wen
authors.forename: Ji-yao
authors.initials: JY
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D012459:Salicylates / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012459:Salicylates; C112485:ginkgolic acid