Sequence-specific 1H, 15N and 13C resonance assignments of Art v 1: a proline-rich allergen of Artemisia vulgaris pollen.
Art v 1 is the major allergen of Artemisia vulgaris. The IgE raised against Art v 1 not only can cross-react with other proteins from the Asteraceae family members but also with components of various forms of food. Art v 1 is an important target for immunotherapy strategies, including vaccination with hypoallergenic derivatives or chimeras. We report the (1)H, (13)C, and (15)N resonance assignments of the recombinant Art v 1 and identification of secondary structures based on (13)C chemical shifts.
Razzera
Guilherme
G
Centro Nacional de Ressonância Magnética Nuclear, Universidade Federal do Rio de Janeiro, Instituto de Bioquímica Médica, Rio de Janeiro, Brazil.
Gadermaier
Gabriele
G
Almeida
Marcius S
MS
Ferreira
Fatima
F
Almeida
Fabio C L
FC
Valente
Ana Paula
AP
D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D002247:Carbon Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*; D011392:Proline / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D011522:Protons
D000485:Allergens; D052179:Antigens, Plant; C102724:Art v 1 protein, mugwort; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010940:Plant Proteins; D021122:Protein Subunits; D011522:Protons; D011392:Proline