[Suppressive effect of resveratrol on growth of U251 human glioma cells and its correlated mechanism].
OBJECTIVE
To investigate the suppressive effect of resveratrol on growth of U251 human glioma cells and its correlated mechanism.


METHODS
U251 human glioma cells were treated with resveratrol at various concentrations, MTT assay was used to determine the inhibitory rate of cell proliferation, FCM to detect the cell apoptosis, the expressions of Bcl-2, Bcl-XL, STAT3 and CyclinD1 were analysed by immunohistochemistry and Western blot to examine the expression of Bcl-2, Bcl-XL, STAT3, CyclinD1, Caspase-3 and Bax.


RESULTS
After treatment with resveratrol, MTT assay showed the growth of U251 cells was inhibited in dose-dependent and time-dependent manners, apoptosis of cells advanced stage was built up, immunohistochemical staining displayed decreased the expression of Bcl-2, Bcl-XL, STAT3 and CyclinD1 and Western blot showed that resveratrol decreased the expression of Bcl-2, Bcl-XL, STAT3 and CyclinD1, and built up Bax and Caspase-3.


CONCLUSIONS
It is possible that downregulated the expression of Bcl-2, Bcl-XL, but upregulated Bax and Caspase-3, and the indication was obviously in dose-dependent and time-dependent manners.
Liu
Hongsheng
H
Key Lab for Critical Care Medicine of Ministry of Health, Tianjin First Central Hospital, Tianjin 300192, China.
Wang
Jinhuan
J
Xu
Xinnv
X
Lin
Na
N
Zhang
Biao
B
Feng
Xuequan
X
Luo
Zhongqiu
Z
D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D000077185:Resveratrol; D050796:STAT3 Transcription Factor / Q000378:metabolism; D013267:Stilbenes / Q000627:therapeutic use*; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
D019253:Proto-Oncogene Proteins c-bcl-2; D050796:STAT3 Transcription Factor; D013267:Stilbenes; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D019938:Cyclin D1; D053148:Caspase 3; D000077185:Resveratrol