Surplus dietary tryptophan inhibits stress hormone kinetics and induces insulin resistance in pigs.
Recently we have shown that surplus dietary tryptophan (TRP) reduced the plasma concentrations of cortisol and noradrenaline in pigs. Stress hormones are known to affect insulin sensitivity and metabolism. We now investigated the long-term effects of surplus dietary TRP on 1) plasma and urinary stress hormone kinetics, 2) insulin sensitivity for glucose and amino acid clearance, and 3) whole body nitrogen balance. Pigs were fed for 3weeks a high (13.2%) vs normal (3.4%) TRP to large neutral amino acids (LNAA) diet, leading to reduced fasting (14 h) plasma cortisol (17.1+/-3.0 vs 28.9+/-4.3 ng/mL, p<0.05) and noradrenaline (138+/-14 vs 225+/-21 pg/mL, p<0.005) concentrations, lower daily urinary noradrenaline (313+/-32 vs 674+/-102 ng/kg day, p<0.001) and adrenaline (124+/-13 vs 297+/-42 ng/kg day, p<0.001) but higher dopamine (5.8+/-0.5 vs 1.5+/-0.2 microg/kg day, p<0.001) excretions, respectively. Insulin sensitivities for both glucose and amino acid clearance, (as measured by the intraportal hyperinsulinaemic (1 mU/kg min) euglycaemic euaminoacidaemic clamp technique), were lower by 22% in pigs on the high vs normal TRP/LNAA diet (14.8+/-1.4 vs 18.9+/-0.9, p<0.05 and 69.7+/-4.3 vs 89.7+/-6.8 mL/kg min, p<0.05, respectively) without affecting urinary nitrogen excretion (35.5+/-1.0 vs 36.6+/-1.0% of dietary nitrogen intake, p=ns). In conclusion, long-term feeding of surplus dietary TRP inhibits both baseline adrenocortical and sympathetic nervous system activity, it induces insulin resistance for both glucose and amino acid clearance but it does not affect whole body protein catabolism. This indicates that the bioactive amino acid TRP contributes to homeostasis in neuroendocrinology and insulin action and that low baseline adrenocortical and sympatho-adrenal axis activity are associated with insulin resistance.
Koopmans
Sietse Jan
SJ
BioMedical Research of Wageningen University and Research Centre, 8219 PH Lelystad, The Netherlands. sietsejan.koopmans@wur.nl
Ruis
Marko
M
Dekker
Ruud
R
Korte
Mechiel
M
D000596:Amino Acids / Q000097:blood; D021542:Amino Acids, Neutral / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002395:Catecholamines; D019587:Dietary Supplements; D004298:Dopamine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000097:blood*; D015309:Glucose Clamp Technique / Q000379:methods; D006854:Hydrocortisone / Q000097:blood / Q000652:urine; D007333:Insulin Resistance / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism; D009638:Norepinephrine / Q000097:blood*; D012463:Saliva / Q000378:metabolism; D013552:Swine; D014364:Tryptophan / Q000008:administration & dosage*
D000596:Amino Acids; D021542:Amino Acids, Neutral; D001786:Blood Glucose; D002395:Catecholamines; D014364:Tryptophan; D009584:Nitrogen; D004298:Dopamine; D006854:Hydrocortisone; D009638:Norepinephrine; D004837:Epinephrine