Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone.
A Gram-positive, moderately halophilic, alkalitolerant, filamentous, aerobic actinomycete, designated strain JSM 073097(T), was isolated from a sea anemone collected from a tidal flat in the South China Sea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the new isolate was a member of the genus Nocardiopsis and was most closely related to Nocardiopsis kunsanensis HA-9(T), Nocardiopsis xinjiangensis YIM 90004(T) and Nocardiopsis salina YIM 90010(T) (99.6, 98.5 and 98.1 % similarity, respectively). Phenotypic characteristics and chemotaxonomic data also indicated that strain JSM 073097(T) was a member of the genus Nocardiopsis. The strain grew well on most of the media tested, producing white to yellow-white substrate mycelium and white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight to flexuous spore chains with non-motile, smooth-surfaced, rod-shaped spores. The strain grew in the presence of 1-15 % (w/v) total salts and at pH 6.0-10.5 and 20-35 degrees C; optimum growth occurred in the presence of 5-7 % (w/v) total salts and at pH 8.5 and 25 degrees C. Whole-cell hydrolysates of strain JSM 073097(T) contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H(4)), MK-10(H(6)) and MK-10(H(8)). The major cellular fatty acids were iso-C(15 : 0), iso-C(16 : 0), anteiso-C(16 : 0) and 10-methyl C(18 : 0). Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine and phosphatidylglycerol. The DNA G+C content of strain JSM 073097(T) was 70.4 mol%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain JSM 073097(T) represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis litoralis sp. nov. is proposed. The type strain is JSM 073097(T) (=DSM 45168(T)=KCTC 19473(T)).
Chen
Yi-Guang
YG
College of Biology and Environmental Sciences, Jishou University, Jishou 416000, People's Republic of China.
Wang
Yong-Xia
YX
Zhang
Yu-Qin
YQ
Tang
Shu-Kun
SK
Liu
Zhi-Xiong
ZX
Xiao
Huai-Dong
HD
Xu
Li-Hua
LH
Cui
Xiao-Long
XL
Li
Wen-Jun
WJ
D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012615:Sea Anemones / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride