title: Development of a two-color fluorescence in situ hybridization technique for species-level identification of human-infectious Cryptosporidium spp.
abstract: A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Alagappan
authors.forename: P L
authors.initials: PL
authors.lastname: Bergquist
authors.forename: B C
authors.initials: BC
authors.lastname: Ferrari
mesh_terms: D000818:Animals; D003116:Color; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*