title: Development and assessment of novel all-in-one parenteral formulations with integrated anticoagulant properties for the concomitant delivery of 5-fluorouracil and calcium folinate.
abstract: 5-Fluorouracil in combination with its biomodulator folinic acid maintains a pivotal position in current anticancer treatment regimens. However, limitations in clinical management persist with the administration of these drugs. These limitations are associated with the use of a high pH to maintain 5-fluorouracil in solution, resulting in high rates of phlebitis and catheter blockages. Herein, we describe and compare initial studies on novel all-in-one formulations of 5-fluorouracil and folinic acid incorporating either sulfated or hydroxypropyl beta-cyclodextrins at physiological pH that potentially address these issues. All formulations markedly improved the stability of supersaturated solutions of 5-fluorouracil in the presence of folinic acid. In-vitro evaluation of the PC-3, HCT-116, MDA-MB-231, PC-14, and COLO-201 human carcinoma cell lines showed that all formulations exhibited equivalent or better cytotoxicity compared with cells exposed to 5-fluorouracil and folinic acid. Thus, these cyclodextrins do not compromise the cytotoxicity of 5-fluorouracil. Preliminary in-vivo dose tolerance profiles of the formulations were also equivalent to 5-fluorouracil and folinic acid administered separately. Furthermore, given the association between thrombosis and cancer, the potentially beneficial anticoagulant activity of the sulfated cyclodextrin-based formulations was also confirmed in vitro. Extended activated partial thromboplastin times and prothrombin times were observed for the sulfated cyclodextrins in human plasma both as individual compounds and as components of the formulations. In conclusion, these novel all-in-one formulations maintain the in-vitro potency while overcoming the accepted incompatibility of 5-fluorouracil and folinic acid, and represent improved injectable forms of 5-fluorouracil that may reduce phlebitis, catheter blockages, and thromboembolic events.
authors.affiliation: School of Chemistry, University of Wollongong, Wollongong, New South Wales, Australia.
authors.forename: Julie M
authors.initials: JM
authors.lastname: Locke
authors.forename: Tamantha K
authors.initials: TK
authors.lastname: Stutchbury
authors.forename: Kara L
authors.initials: KL
authors.lastname: Vine
authors.forename: Allan B
authors.initials: AB
authors.lastname: Gamble
authors.forename: Philip R
authors.initials: PR
authors.lastname: Clingan
authors.forename: John B
authors.initials: JB
authors.lastname: Bremner
authors.forename: Marie
authors.initials: M
authors.lastname: Ranson
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage*; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003505:Cyclodextrins / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity; D004338:Drug Combinations; D005079:Excipients / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D006801:Humans; D007263:Infusions, Parenteral; D002955:Leucovorin / Q000008:administration & dosage* / Q000494:pharmacology / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000925:Anticoagulants; D000964:Antimetabolites, Antineoplastic; D003505:Cyclodextrins; D004338:Drug Combinations; D005079:Excipients; D002955:Leucovorin; D005472:Fluorouracil