Association of the C-399T promoter polymorphism of neuropeptide Y with susceptibility to ischemic stroke.
OBJECTIVE
The common sequence variants of neuropeptide Y (NPY) were known to be associated with some kinds of diseases including stroke. This study investigated the association of NPY promoter polymorphism, C-399T, with ischemic stroke and its underlying mechanism using in vitro systems.


METHODS
Study subjects consisted of 444 ischemic stroke patients and 326 controls without stroke. C-399T genotyping was conducted by a primer extension-based method. Plasma NPY was quantified with an enzyme immunoassay, and transcription characteristics were investigated by a reporter gene assay and an enzyme mobility shift assay.


RESULTS
A significantly lower frequency of TT genotype was observed in a stroke group (OR[95% CI], 0.399[0.187-0.854], p=0.0180). The C-399T polymorphism affected the transcription efficiency of NPY gene and its genotypes were related to the changes in plasma NPY levels.


CONCLUSIONS
This study suggests that NPY promoter polymorphism, C-399T, should be considered a genetic risk factor for ischemic stroke.
Kim
No S
NS
Brain Disease Research Center, Division of Traditional Korean Medicine Integrated Research, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejeon, Republic of Korea.
Oh
Se-Mi
SM
Ko
Mi M
MM
Cha
Min H
MH
Kang
Byoung K
BK
Bang
Ok S
OS
D000368:Aged; D001483:Base Sequence; D002545:Brain Ischemia / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009478:Neuropeptide Y / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D020521:Stroke / Q000235:genetics*
D017931:DNA Primers; D009478:Neuropeptide Y