Medium optimization for the production of a novel bioflocculant from Halomonas sp. V3a' using response surface methodology.
The novel exopolysaccharide bioflocculant HBF-3 is produced by Halomonas sp. V3a', which is a mutant strain of the deep-sea bacterium Halomonas sp. V3a. Response surface methodology (RSM) was employed to optimize the production medium for increasing HBF-3 production. Using a Plackett-Burman experimental design to aid in the first step of optimization, edible glucose, MgSO(4) x 7 H(2)O, and NH(4)Cl were found to be significant factors affecting HBF-3 production. To determine the optimal concentration of each significant variable, a central composite design was employed. Based on response surface and canonical analysis, the optimum concentrations of the critical components were obtained as follows: edible glucose, 16.14 g/l; MgSO(4) x 7 H(2)O, 2.73 g/l; and NH(4)Cl, 1.97 g/l. HBF-3 production obtained by using the optimized medium was 4.52 g/l, which was in close agreement with the predicted value of 4.55 g/l. By scaling up fermentation from flask to fermenter, HBF-3 production was further increased to 5.58 g/l.
He
Jin
J
State Key Laboratory of Agricultural Microbiology and National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, PR China.
Zhen
Quanwei
Q
Qiu
Ning
N
Liu
Ziduo
Z
Wang
Baojiang
B
Shao
Zongze
Z
Yu
Ziniu
Z
D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D020632:Halomonas / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D008829:Microbiology; D015233:Models, Statistical; D011134:Polysaccharides / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D012044:Regression Analysis; D013499:Surface Properties
D003470:Culture Media; D004275:DNA, Ribosomal; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S; D005947:Glucose