title: Biosurfactant production by Pseudomonas sp. and its role in aqueous phase partitioning and biodegradation of chlorpyrifos.
abstract: OBJECTIVE
  To study the effect of biosurfactant on aqueous phase solubility and biodegradation of chlorpyrifos.
  
  
  RESULTS
  A Pseudomonas sp. (ChlD), isolated from agricultural soil by enrichment culture technique in the presence of chlorpyrifos, was capable of producing biosurfactant (rhamnolipids) and degrading chlorpyrifos (0.01 g l(-1)). The partially purified rhamnolipid biosurfactant preparation, having a CMC of 0.2 g l(-1), was evaluated for its ability to enhance aqueous phase partitioning and degradation of chlorpyrifos (0.01 g l(-1)) by ChlD strain. The best degradation efficiency was observed at 0.1 g l(-1) supplement of biosurfactant, as validated by GC and HPLC studies.
  
  
  CONCLUSIONS
  The addition of biosurfactant at 0.1 g l(-1) resulted in more than 98% degradation of chlorpyrifos when compared to 84% in the absence of biosurfactant after 120-h incubation.
  
  
  CONCLUSIONS
  This first report, to the best of our knowledge, on enhanced degradation of chlorpyrifos in the presence of biosurfactant(s), would help in developing bioremediation protocols to counter accumulation of organophosphates to toxic/carcinogenic levels in environment.
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, India.
authors.forename: P B
authors.initials: PB
authors.lastname: Singh
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: H S
authors.initials: HS
authors.lastname: Saini
authors.forename: B S
authors.initials: BS
authors.lastname: Chadha
mesh_terms: D001711:Biotransformation; D004390:Chlorpyrifos / Q000737:chemistry / Q000378:metabolism*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006017:Glycolipids / Q000302:isolation & purification / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*; D012995:Solubility; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid; D004390:Chlorpyrifos