Genetically different isolates of Trypanosoma cruzi elicit different infection dynamics in raccoons (Procyon lotor) and Virginia opossums (Didelphis virginiana).
Trypanosoma cruzi is a genetically and biologically diverse species. In the current study we determined T. cruzi infection dynamics in two common North American reservoirs, Virginia opossums (Didelphis virginiana) and raccoons (Procyon lotor). Based on previous molecular and culture data from naturally-exposed animals, we hypothesised that raccoons would have a longer patent period than opossums, and raccoons would be competent reservoirs for both genotypes T. cruzi I (TcI) and TcIIa, while opossums would only serve as hosts for TcI. Individuals (n=2 or 3) of each species were inoculated with 1x10(6) culture-derived T. cruzi trypomastigotes of TcIIa (North American (NA) - raccoon), TcI (NA - opossum), TcIIb (South American - human), or both TcI and TcIIa. Parasitemias in opossums gradually increased and declined rapidly, whereas parasitemias peaked sooner in raccoons and they maintained relatively high parasitemia for 5weeks. Raccoons became infected with all three T. cruzi strains, while opossums only became infected with TcI and TcIIb. Although opossums were susceptible to TcIIb, infection dynamics were dramatically different compared with TcI. Opossums inoculated with TcIIb seroconverted, but parasitemia duration was short and only detectable by PCR. In addition, raccoons seroconverted sooner (3-7days post inoculation) than opossums (10days post inoculation). These data suggest that infection dynamics of various T. cruzi strains can differ considerably in different wildlife hosts.
Roellig
Dawn M
DM
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30606, USA. droellig@uga.edu
Ellis
Angela E
AE
Yabsley
Michael J
MJ
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology* / Q000662:veterinary; D048293:Didelphis / Q000469:parasitology*; D004197:Disease Reservoirs / Q000469:parasitology / Q000662:veterinary; D005260:Female; D006790:Host-Parasite Interactions; D008297:Male; D018512:Parasitemia / Q000662:veterinary; D011821:Raccoons / Q000469:parasitology*; D011897:Random Allocation; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000302:isolation & purification