Cloning and expression of cellulase gene EG1 from Rhizopus stolonifer var. reflexus TP-02 in Escherichia coli.
A novel gene, EG encoding enzymes involved in carboxymethyl cellulose (CMC) degradation was isolated, sequenced from the filamentous fungus Rhizopus stolonifer var. reflexus TP-02, and expressed in Escherichia coli BL21. The results showed that the gene amplified from the cDNA of the strain could be classified as the family of endoglucanase. During the fermentation process, the maximum endoglucanase activity (i.e. 0.715 IU/ml) of the recombinant bacteria was obtained at 36 h. The SDS-PAGE analysis on purified samples showed that a band with apparent molecular weight of about 40 kDa was detected after staining with Coomassie brilliant blue.
Tang
Bin
B
Department of Biochemical Engineering, Anhui University of Technology and Science, Wuhu 241000, China. tangbin@auts.edu.cn
Pan
Haibo
H
Zhang
Qingqing
Q
Ding
Lixia
L
D002266:Carboxymethylcellulose Sodium / Q000737:chemistry / Q000378:metabolism; D002480:Cellulase / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005285:Fermentation; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics*; D013997:Time Factors
D018076:DNA, Complementary; D012333:RNA, Messenger; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium