Identification of chromosome abnormalities in the horse using a panel of chromosome-specific painting probes generated by microdissection.
Fluorescent in situ hybridisation (FISH) using a panel of molecular probes for all chromosome pairs obtained by chromosome microdissection of the domestic horse ( Equus caballus ) was used to diagnose karyotype abnormalities in 35 horses (32 mares, 2 stallions and 1 intersex), which were selected for the study due to infertility (23 horses), reduced fertility (10 horses) and developmental anomalies (2 horses). The use of the FISH technique with probes for each horse chromosome pair enabled the diagnosis of many different chromosome aberrations in this population. Among the horses analysed, 21 animals had normal karyotype - 64,XX (19 mares) and 64,XY (2 stallions). Fourteen animals, constituting 40% of the population studied, showed the following chromosome abnormalities: 63,X (1 mare); 63,X/64,XX (6 mares); 63,X/64,XX/65,XXX (3 mares); 63,X/65,XXX (1 mare); 64,XX/65,XX+Xp (1 mare); 63,X/64,XX/65,XX+Xq (1 mare), and 63,X/64,XX/65,XX+delY (1 intersex). When only the mares studied because of complete infertility were taken into consideration, this proportion exceeded 56%. Due to the increased frequency of the above-mentioned aberrations in the mosaic form of two or more lines, it was necessary to analyse a large number (100-300) of metaphase spreads. The use of specific molecular probes obtained by chromosome microdissection made these diagnoses much easier.
Bugno
Monika
M
Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków, Poland. mbugno@izoo.krakow.pl
Słota
Ewa
E
Pieńkowska-Schelling
Aldona
A
Schelling
Claude
C
D000818:Animals; D020223:Chromosome Painting / Q000379:methods / Q000662:veterinary*; D012734:Disorders of Sex Development / Q000235:genetics / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D007246:Infertility / Q000235:genetics / Q000662:veterinary*; D007621:Karyotyping / Q000662:veterinary; D008297:Male; D042282:Microdissection / Q000379:methods / Q000662:veterinary*; D012729:Sex Chromosome Aberrations / Q000662:veterinary*