[Method for drainage of lymph fluid and determining the change of active materials in lymph fluid after rat ischemia-reperfusion injury].
OBJECTIVE
To set up a method for the drainage of lymph fluid and explore the change of active materials in lymph fluid and serum after rat ischemia-reperfusion injury.


METHODS
The method of the drainage of lymph fluid was well established. Sixteen healthy male rats of SPF grade were selected and randomly divided into 2 groups: intestinal ischemia-reperfusion + drainage group (I/R + drainage group) and drainage group. All the rats were subjected to superior mesenteric artery occlusion for 60 minutes, followed by 120 minutes of reperfusion. We compared the change of high mobility group box-1 (HMGB1) protein, endotoxin tumor necrosis factor alpha (TNF-alpha), interleukin (IL) -1 beta, IL-6, and soluble vascular cell adhesion molecular-1 (sICAM-1) by draining lymph fluid and collecting serum in 2 groups.


RESULTS
The drainage of lymph fluid was successfully performed. The HMGB1, endotoxin, and cytokines in serum and lymph fluid were significantly higher in ischemia-reperfusion group than in drainage group (P < 0. 05).


CONCLUSIONS
The method for drainage of lymph fluid is simple and feasible. Endotoxin, HMGB1, and some cytokines in serum and lymph fluid may mediate the ischemia-reperfusion injury.
Chen
Xue-Feng
XF
Department of Enteral and Parenteral Nutrition, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
He
Gui-Zhen
GZ
Dong
Liang-Guang
LG
Shu
Hong
H
Wang
Xiu-Rong
XR
Gao
Feng-Yan
FY
D000818:Animals; D004195:Disease Models, Animal; D004322:Drainage / Q000379:methods; D004731:Endotoxins / Q000378:metabolism; D024243:HMGB1 Protein / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D008196:Lymph / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004731:Endotoxins; D024243:HMGB1 Protein; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha