title: Erroneous reporting of vancomycin susceptibility for Staphylococcus spp. by Vitek software version 2.01.
abstract: The reporting of vancomycin resistance in Staphylococcus spp. has enormous therapeutic and epidemiological consequences. We evaluated Vitek software version 2.01 by the CLSI-recommended broth dilution method as well as the CDC-recommended vancomycin screen agar and Etest for vancomycin susceptibility testing of Staphylococcus spp. Of the 105 isolates of Staphylococci tested by the above methods, Vitek 2.01 gave 16 (15%) a false vancomycin intermediate/resistant phenotype. Laboratories using automated systems for routine microbiological susceptibility testing must confirm such resistance by validated methods.
authors.affiliation: Microbiology Division, Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Bijayini
authors.initials: B
authors.lastname: Behera
authors.forename: Purva
authors.initials: P
authors.lastname: Mathur
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003951:Diagnostic Errors; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000187:drug effects* / Q000302:isolation & purification; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin