title: [Rapid establishment of suspension cell lines in japonica rice].
abstract: With Jingkang No.5 (PiA), calli of the PiA induced for 10-15 days were transferred into amino acid liquid culture medium, to establish excellent rice suspension cell lines successfully in a relative short time. The growth characteristics and differentiation conditions of suspension cells were measured at different phases. Results revealed that the optimal subculture time was 7-10 days, and the cells cultured for 30-120 days had the best differentiation ability (57.1%) and regeneration ability (20%). This study is promising in further using the suspension cell for genetic transformation and protoplasm isolation.
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.forename: Fuyun
authors.initials: F
authors.lastname: Yang
authors.forename: Mei
authors.initials: M
authors.lastname: Gao
authors.forename: Peizhou
authors.initials: P
authors.lastname: Xu
authors.forename: Quanfang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jiandi
authors.initials: J
authors.lastname: Xu
authors.forename: Xianjun
authors.initials: X
authors.lastname: Wu
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D003470:Culture Media; D012275:Oryza / Q000166:cytology*
chemical_list: D003470:Culture Media