Remyelination in experimentally demyelinated connexin 32 knockout mice.
The aim of this study was to evaluate the role of connexin 32 (Cx 32) during remyelination of the peripheral nervous system, through a local injection of either 0,1% ethidium bromide solution or saline in the sciatic nerve of Cx 32 knockout mice. Euthanasia was performed ranging from 1, 2, 3, 7, 15, 21 to 30 days after injection. Histochemical, immunohistochemical, immunofluorescence and transmission electron microscopical techniques were used to analyze the development of the lesions. Within the sciatic nerves, Schwann cells initially showed signs of intoxication and rejected their sheaths; after seven days, some thin newly formed myelin sheaths with uneven compactness and redundant loops (tomacula) were conspicuous. We concluded that the regeneration of lost myelin sheaths within the PNS followed the pattern already reported for this model in other laboratory species. Therefore, these results suggest that absence of Cx 32 did not interfere with the normal pattern of remyelination in this model in young mice.
Ramos
Adriano Tony
AT
Laboratory of Veterinary Pathology, Departament of Pathology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Maiorka
Paulo César
PC
Dagli
Maria Lúcia Zaidan
ML
Hosomi
Fernando Yutaka Moniwa
FY
Violin
Kalan Bastos
KB
Latorre
Andréa
A
Viott
Aline de Marco
Ade M
Masuda
Eduardo Kenji
EK
Trost
Maria Elisa
ME
Martins
Tessie Beck
TB
Graça
Dominguita Lühers
DL
D000818:Animals; D017630:Connexins / Q000502:physiology*; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000503:physiopathology*; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D009186:Myelin Sheath / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D000097002:Gap Junction beta-1 Protein
D017630:Connexins; D000097002:Gap Junction beta-1 Protein