First structural characterization of Burkholderia vietnamiensis lipooligosaccharide from cystic fibrosis-associated lung transplantation strains.
This is the first structural elucidation of the lipooligosaccharide (LOS) endotoxin isolated from Burkholderia vietnamiensis, a clinically important member of Burkholderia cepacia complex, a group of over 10 opportunistic species that are highly problematic in cystic fibrosis. We have characterized a novel LOS structure extracted from two clonal strains of B. vietnamiensis isolated from a cystic fibrosis patient who underwent lung transplantation. Strains were selected from the pretransplantation and post-transplantation periods and endotoxin was extracted. Subsequent analysis interestingly revealed identical oligosaccharidic sequences, but variation in lipid A moieties. Further, both LOS fractions were tested for their immunostimulatory activity on human myelomonocytic U937 cells and for signaling on an HEK293 cell line stably expressing both TLR 4 and MD-2. We observed an increase in lipid A acylation and a resultant increase in biological activity in bio-reporter assays of TNF-alpha secretion in the post-transplantation strain.
Ieranò
Teresa
T
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Napoli, Italy.
Silipo
Alba
A
Sturiale
Luisa
L
Garozzo
Domenico
D
Bryant
Clare
C
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
Aldridge
Christine
C
Gould
F Kate
FK
Corris
Paul A
PA
Khan
C M Anjam
CM
De Soyza
Anthony
A
Molinaro
Antonio
A
D019117:Burkholderia / Q000737:chemistry* / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016040:Lung Transplantation; D008969:Molecular Sequence Data
D008070:Lipopolysaccharides