title: Epidemiology of malaria in an area prepared for clinical trials in Korogwe, north-eastern Tanzania.
abstract: BACKGROUND
  Site preparation is a pre-requesite in conducting malaria vaccines trials. This study was conducted in 12 villages to determine malariometric indices and associated risk factors, during long and short rainy seasons, in an area with varying malaria transmission intensities in Korogwe district, Tanzania. Four villages had passive case detection (PCD) of fever system using village health workers.
  
  
  METHODS
  Four malariometric cross-sectional surveys were conducted between November 2005 and May 2007 among individuals aged 0-19 years, living in lowland urban, lowland rural and highland strata. A total of 10,766 blood samples were collected for malaria parasite diagnosis and anaemia estimation. Blood smears were stained with Giemsa while haemoglobin level was measured by HaemoCue. Socio-economic data were collected between Jan-Apr 2006.
  
  
  RESULTS
  Adjusting for the effect of age, the risk of Plasmodium falciparum parasitaemia was significantly lower in both lowland urban, (OR = 0.26; 95%CI: 0.23-0.29, p < 0.001) and highlands, (OR = 0.21; 95%CI: 0.17-0.25, p < 0.001) compared to lowland rural. Individuals aged 6-9 years in the lowland rural and 4-19 years in both lowland urban and highlands had the highest parasite prevalence, whilst children below five years in all strata had the highest parasite density. Prevalence of splenomegaly and gametocyte were also lower in both lowland urban and highlands than in lowland rural. Anaemia (Hb <11 g/dl) prevalence was lowest in the lowland urban. Availability of PCD and higher socio-economic status (SES) were associated with reduced malaria and anaemia prevalence.
  
  
  CONCLUSIONS
  Higher SES and use of bed nets in the lowland urban could be the important factors for low malaria infections in this stratum. Results obtained here were used together with those from PCD and DSS in selecting a village for Phase 1b MSP3 vaccine trial, which was conducted in the study area in year 2008.
authors.affiliation: National Institute for Medical Research, Tanga Medical Research Centre, Tanga, Tanzania. bmmbando@tanga.mimcom.net
authors.forename: Bruno P
authors.initials: BP
authors.lastname: Mmbando
authors.forename: Method D
authors.initials: MD
authors.lastname: Segeja
authors.forename: Hamisi A
authors.initials: HA
authors.lastname: Msangeni
authors.forename: Samwel H
authors.initials: SH
authors.lastname: Sembuche
authors.forename: Deus S
authors.initials: DS
authors.lastname: Ishengoma
authors.forename: Misago D
authors.initials: MD
authors.lastname: Seth
authors.forename: Filbert
authors.initials: F
authors.lastname: Francis
authors.forename: Acleus S
authors.initials: AS
authors.lastname: Rutta
authors.forename: Mathias L
authors.initials: ML
authors.lastname: Kamugisha
authors.forename: Martha M
authors.initials: MM
authors.lastname: Lemnge
mesh_terms: D000293:Adolescent; D017677:Age Distribution; D000740:Anemia / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D000818:Animals; D002648:Child; D002675:Child, Preschool; D002986:Clinical Trials as Topic; D003430:Cross-Sectional Studies; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017780:Malaria Vaccines; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D018512:Parasitemia / Q000097:blood* / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D015995:Prevalence; D012307:Risk Factors; D012424:Rural Population; D012959:Socioeconomic Factors; D013163:Splenomegaly / Q000175:diagnosis / Q000453:epidemiology; D013636:Tanzania / Q000453:epidemiology; D055815:Young Adult
chemical_list: D006454:Hemoglobins; D017780:Malaria Vaccines