[Effects of Tianma Xingnao capsule on thrombosis and coagulation system].
OBJECTIVE
This study investigated the thrombolitic effect of Tianma Xinnao capsule and its preliminary mechanism, as well as the effect on coagulation system.


METHODS
Charlton's and Tomihisa's methods were modified to investigate the thrombolytic effect of Tianma Xinnao capsule. The activities of type 1 plasminogen activator inhibitor (PAI-1) and tissue-type plasminogen activator (tPA) in rabbit plasma were assayed by use of ELISA. The effects of Tianma Xinnao capsule were also evaluated on euglobulin lysis time (ELT), prothrombin time (PT), kaolin partial thromboplastin time (KPTT), thrombin time (TT), fibrinogen (Fib) and hemorheology.


RESULTS
The results showed that Tianma Xinnao capsule had a dose-dependent thrombolytic effect in rats. Tianma Xinnao capsule at 0.6 and 1.2 g x kg(-1) produced 40% and 50% of reperfusion rate, while obtained 50% and 40% of reocclusion rate; 0.3 g x kg(-1) of Tianma Xinnao capsule, however, had no effect on the reperfusion or reocclusion rate. Tianma Xinnao capsule significantly inhibited PAI-1 activity, while elevated tPA activity in rabbit plasma. Tianma Xinnao capsule markedly prolonged ELT, PT, and KPTT, and decreased Fib level. Tianma Xinnao capsule showed no significant influence on TT or high, medium, or low sheering viscosity.


CONCLUSIONS
It is indicated that Tianma Xinnao capsule inhibited PAI-1 activity and increased tPA activity, and this property of Tianma Xinnao capsule is assigned to be responsible for the thrombolytic effect.
Yang
Jianyu
J
Department of Pharmacology, Haiyuan College of Kunming Medical College, Kunming 650106, China.
He
Bo
B
Yang
Yang
Y
Zhan
Xiaochao
X
Zhen
Jindan
J
Shen
Zhiqiang
Z
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001809:Blood Viscosity / Q000187:drug effects; D002214:Capsules; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D005260:Female; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013927:Thrombosis / Q000097:blood* / Q000188:drug therapy* / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism
D002214:Capsules; D004365:Drugs, Chinese Herbal; D017395:Plasminogen Activator Inhibitor 1; C543120:tianma xingnao; D010959:Tissue Plasminogen Activator