title: Fatty acid profiling and proteomic analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide.
abstract: Non-thermal sterilization and microbial inactivation processes are currently receiving much attention in food and pharmaceutical industries. In particular, since supercritical carbon dioxide (SC-CO2) treatment, which is conducted at relatively low temperatures, is considered to be a promising alternative method to replace thermal sterilization processes that cannot be safely used in foods and bioactive materials. Although SC-CO2 has been applied to many microorganisms, the inactivation of microbial cells by SC-CO2 has only been evaluated by using a conventional viable cell count such as a plating method, by which it is not possible to systematically elucidate the microbial cell inactivation process. Therefore, in this study the physiological status of SC-CO2 treated Salmonella enterica serotype Typhimurium was analyzed by using GC-MS analysis of fatty acids with principal component analysis and two-dimensional electrophoresis for protein profiling. From the results of these systemic analyses, it was revealed that SC-CO2 caused significant alterations to the profiles of fatty acids and proteins of the cells.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Soo Rin
authors.initials: SR
authors.lastname: Kim
authors.forename: Hee Taek
authors.initials: HT
authors.lastname: Kim
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Sooah
authors.initials: S
authors.lastname: Kim
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Yi
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Ryu
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D005516:Food Microbiology; D008401:Gas Chromatography-Mass Spectrometry; D050296:Microbial Viability / Q000187:drug effects; D025341:Principal Component Analysis; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000378:metabolism*; D013242:Sterilization / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D020543:Proteome; D002245:Carbon Dioxide