title: An accurate and rapid flow cytometric diagnosis of BCR-ABL positive acute lymphoblastic leukemia.
abstract: Tyrosine kinase inhibitors have profoundly modified the treatment and prognosis of chronic myeloid leukemia and Ph(+) acute lymphoblastic leukemia. A rapid and accurate detection of the BCR-ABL fusion protein is paramount today for an optimal management of Ph(+) acute lymphoblastic leukemia. We have utilized a recently described and commercialized immunoassay that identifies qualitatively the presence of the BCR-ABL protein in leukemic cell lysates. The BCR-ABL fusion protein is captured and detected by a cytometric bead assay and analyzed by flow cytometry. The assay was applied to 101 primary patient samples (94 acute leukemias and 7 chronic myeloid leukemia blast crisis) and the results of the immunoassay were concordant with those obtained by conventional molecular techniques. The method proved reliable, reproducible, of simple execution and it was successfully completed within four hours. This flow cytometric immunoassay has important implications for perfecting the management of Ph(+) acute lymphoblastic leukemia patients worldwide.
authors.affiliation: Division of Hematology, Department of Cellular Biotechnologies and Hematology, Sapienza University of Rome, Rome, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Raponi
authors.forename: Maria Stefania
authors.initials: MS
authors.lastname: De Propris
authors.forename: Hobert
authors.initials: H
authors.lastname: Wai
authors.forename: Stefania
authors.initials: S
authors.lastname: Intoppa
authors.forename: Loredana
authors.initials: L
authors.lastname: Elia
authors.forename: Daniela
authors.initials: D
authors.lastname: Diverio
authors.forename: Antonella
authors.initials: A
authors.lastname: Vitale
authors.forename: Robin
authors.initials: R
authors.lastname: Foà
authors.forename: Anna
authors.initials: A
authors.lastname: Guarini
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007223:Infant; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D016044:Fusion Proteins, bcr-abl