title: Simultaneous quantification of eight major bioactive phenolic compounds in Chinese propolis by high-performance liquid chromatography.
abstract: A simple, sensitive and specific high-performance liquid chromatography-UV (HPLC-UV) method has been developed to simultaneously quantify the eight major bioactive phenolic compounds in Chinese propolis, namely caffeic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin 5-methyl ether, pinocembrin, benzyl caffeate, chrysin and galangin. This HPLC assay was performed on an Agilent Zorbax Extend-C18 (250 x 4.6 mm, 5 microm) column with a gradient of methanol and 0.2% aqueous acetic acid (v/v) in 50 min, at a flow rate of 1.0 mL/min, and detected at 290 nm. All calibration curves showed good linearity (r2 > 0.999) within the test ranges. The intra- and inter-day assay precision (RSD) of eight phenolic compounds were in the range of 0.07-4.92%. The recoveries were between 98.3% and 104.8%. This assay was applied to the evaluation of nineteen samples from different origins in China. The results indicated that the developed assay could be readily utilized for the quality control of propolis.
authors.affiliation: College of Traditional Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Sha
authors.forename: Hui-Lian
authors.initials: HL
authors.lastname: Huang
authors.forename: Jin-Qiang
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Guang-Tong
authors.initials: GT
authors.lastname: Chen
authors.forename: Si-Jia
authors.initials: SJ
authors.lastname: Tao
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Xing-Nuo
authors.initials: XN
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry*
chemical_list: D010636:Phenols; D011429:Propolis