Molecular diversity and differential expression of starch-synthesis genes in developing kernels of three maize inbreds.
The maize genome remains abundant in molecular diversity, and the rich genetic diversity of maize starch-synthesis genes is crucial for controlling various grain traits. To explore the unique mechanism controlling the advantageous waxy trait and characterize the molecular feature of genes relevant to starch composition in two elite waxy inbreds, expression profiling combined with gene organization analysis was performed in them as compared to one normal inbred. Genotype-specific expression patterns were observed for most genes studied. The waxy inbreds were shown to contain mutations in multiple starch-synthesis genes, namely gbssI (wx), gbssIIb and isa2 (potentially isa3 too).The mis-splicing events directly accounted for wx loss of function. Contrarily, disruption of 5' and 3' transcript sequence may contribute to the absence of GbssIIb and Isa2 transcripts in waxy inbreds, respectively. Besides, the splicing of Sugary1 transcript was developmentally regulated in the normal inbred, and DNA polymorphisms were detected within SSIIIb-1 gene in waxy inbreds.
Ding
Xiang-Zhen
XZ
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Biological Technique, Shanghai Academy of Agricultural Sciences (SAAS), Beidi Road 2901, Minhang Zone, 201106 Shanghai, China.
Wang
Bei-Guo
BG
Gao
Qing-Hua
QH
Zhang
Qiong
Q
Yan
Gui-Qin
GQ
Duan
Ke
K
Huang
Jian-Hua
JH
D018744:DNA, Plant / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007178:Inbreeding; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013213:Starch / Q000096:biosynthesis*; D013214:Starch Synthase / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins; C060756:waxy protein, plant; D013213:Starch; C434607:granule-bound starch synthase I; D013214:Starch Synthase