Expression pattern and prokaryotic expression for peking duck insulin-like growth factor-I in Escherichia coli.
Insulin-like growth factor-I (IGF-I) is an important regulator of growth, development, and metabolism in animals. This study investigated its expression pattern in various tissues in Peking duck and in vivo. The result showed that the recombinant protein of IGF-I was expressed mainly in the form of inclusion bodies in the Escherichia coli cell (37.4 kD). Western blotting indicated that the recombinant protein had the antigenicity of IGF-I. Reverse-transcript PCR and real-time fluorescent quantitative PCR analyses showed that IGF-I was expressed in the heart, liver, abdominal fat, breast muscle, spleen, lung, kidney, gizzard, and small intestine of 8-week-old birds. In the RT-PCR results, IGF-I was expressed highest in the heart and lung and lowest in abdominal fat and breast muscle in male and female birds. In the real-time fluorescent quantitative PCR results, IGF-I was expressed highest in the gizzard and lowest in the lung in all ducks.
Wu
Yan
Y
Shaanxi Key Laboratory of Molecular Biology for Agriculture, Animal Science and Technology College, Northwest A&F University, Yangling, China. liuxiaolin@nwsuaf.edu.cn
Liu
Xiao-lin
XL
Xiao
Hong-wei
HW
Wang
Jie
J
Jiang
Fei
F
Zhao
Sheng
S
Liu
Yu
Y
D000818:Animals; D004372:Ducks / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D002479:Inclusion Bodies; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008297:Male; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D014018:Tissue Distribution
D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I