[Determination of clopidol residue in poultry products by liquid chromatography-electrospray ion trap tandem mass spectrometry].
A method is presented for the determination of clopidol residue in poultry products by liquid chromatography-electrospray ion trap tandem mass spectrometry (LC-ESI-MS/MS). Clopidol residue was extracted with methanol and cleaned-up by n-hexane from different poultry products. The extract was cleaned-up by an LC-18 column and an ion exchange solid phase extraction (SPE) column. Quantification was achieved by matrix calibration. The recoveries of clopidol in poultry products were in the range from 55.38% to 132.44% at the four spiked levels, 2, 5, 10, and 20 microg/kg. The intra-day relative standard deviation (RSD) and inter-day RSD were less than 9.54% and 15.27%, respectively. The linearity of the method was good from 1 microg/kg to 40 microg/kg. The limit of detection (LOD) was 0.5 microg/kg, and the limit of quantification (LOQ) was 2.0 microg/kg. The method is selective without interference and suitable for the determination and confirmation of clopidol residue in poultry products.
Yang
Wenquan
W
Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China. xiaoquan025@163.com
Xu
Jinzhong
J
Yang
Gongjun
G
Ding
Tao
T
Qi
Juanfei
J
Liu
Han
H
Shen
Chongyu
C
Wu
Bin
B
Yu
Shu
S
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D003007:Clopidol / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D011202:Poultry Products / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D003007:Clopidol