title: Expression of Japanese encephalitis virus envelope protein in transgenic tobacco plants.
abstract: The virus envelope (E) protein of Japanese encephalitis virus induces virus-neutralizing antibodies and is therefore a potential vaccine antigen. In a mammalian system, co-expression of another viral structural protein prM is necessary for proper expression and folding of E protein. Transgenic tobacco plants were produced carrying JEV cDNA encoding prM and E proteins under the control of the CaMV 35S promoter. E protein, however, was not detectable in these plants. In vitro translation studies showed that the presence of the prM sequence inhibited transgene expression in the plant system. Accordingly, JEV E protein could be expressed in transgenic tobacco plants only without the prM protein.
authors.affiliation: National Institute of Immunology and Vaccine and Infectious Disease Research Center, THSTI, New Delhi 110067, India.
authors.forename: Mohan Babu
authors.initials: MB
authors.lastname: Appaiahgari
authors.forename: M Z
authors.initials: MZ
authors.lastname: Abdin
authors.forename: K C
authors.initials: KC
authors.lastname: Bansal
authors.forename: Sudhanshu
authors.initials: S
authors.lastname: Vrati
mesh_terms: D000914:Antibodies, Viral; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D017510:Protein Folding; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus; C074932:prM protein, Flavivirus