A role for alpha11beta1 integrin in the human periodontal ligament.
We previously demonstrated a role for alpha11beta1 integrin in periodontal ligament (PDL)-driven tooth eruption in the mouse. To explore a possible role for alpha11beta1 in the human periodontium, we have characterized the expression and function of alpha11 in human PDL tissue, in human PDL fibroblasts (hPDLF), and in human gingival fibroblasts (hGF). alpha11 expression was detected in PDL tissue, in hPDLF, and in hGF cells. Platelet-derived growth factor-BB and insulin-like growth factor II stimulated contraction of collagen lattices by both types of fibroblasts. alpha2 integrin blocking antibodies and the use of alpha11 siRNA demonstrated a role for both alpha2beta1 and alpha11beta1 in collagen lattice remodeling. Analysis of the proximal ITGA11 promoter from persons with chronic periodontal disease failed to reveal any polymorphism. Analysis of our data shows that alpha11beta1 is a major collagen receptor on cultured human PDL cells and implies that it is also functionally important in the PDL in vivo.
Barczyk
M M
MM
Department of Clinical Dentistry - Periodontics, University of Bergen, Aarstadveien 17, N-5009 Bergen, Norway.
Olsen
L-H Borge
LH
da Franca
P
P
Loos
B G
BG
Mustafa
K
K
Gullberg
D
D
Bolstad
A I
AI
D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D000077214:Becaplermin; D016022:Case-Control Studies; D002478:Cells, Cultured; D055113:Chronic Periodontitis / Q000378:metabolism*; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology; D005881:Gingiva / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D039001:Integrin alpha Chains / Q000037:antagonists & inhibitors / Q000235:genetics; D016023:Integrins / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020574:Proto-Oncogene Proteins c-sis; D034622:RNA Interference; D038981:Receptors, Collagen / Q000235:genetics / Q000502:physiology*; D055815:Young Adult
D024042:Collagen Type I; C121535:ITGA11 protein, human; D039001:Integrin alpha Chains; D016023:Integrins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D038981:Receptors, Collagen; C467800:integrin alpha11beta1; D000077214:Becaplermin; D007335:Insulin-Like Growth Factor II