title: Detection of Shiga toxin-producing Escherichia coli and enteropathogenic Escherichia coli in faecal samples of healthy mithun (Bos frontalis) by multiplex polymerase chain reaction.
abstract: Faecal samples obtained from 190 healthy mithuns were examined for the presence of Escherichia coli. Total one-hundred and five E. coli isolates were obtained from these samples, which belonged to 55 different serogroups. These isolates were subjected to multiplex polymerase chain reaction (m-PCR) for detection of stx1, stx2, eaeA and hlyA genes. Twenty-three (21.90%) E. coli isolates belonging to 14 serogroups revealed the presence of at least one virulence gene when examined by m-PCR. Nineteen percent and 2.85% of the mithuns were found to carry Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli, respectively. stx1 and stx2 genes were found to be prevalent in 7 (6.67%) and 18 (17.14%) of the isolates respectively, whereas eaeA and hlyA genes were found to be carried by three (2.85% each) isolates. Interestingly, none of the STEC isolates belonged to serogroup O157.
authors.affiliation: National Research Centre on Pig, Indian Council of Agricultural Research, Rani, Guwahati, India. swaraj.rajkhowa@gmail.com
authors.forename: S
authors.initials: S
authors.lastname: Rajkhowa
authors.forename: R
authors.initials: R
authors.lastname: Das
authors.forename: S
authors.initials: S
authors.lastname: Bora
authors.forename: C
authors.initials: C
authors.lastname: Rajkhowa
authors.forename: H
authors.initials: H
authors.lastname: Rahman
authors.forename: K M
authors.initials: KM
authors.lastname: Bujarbaruah
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D054308:Enteropathogenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005243:Feces / Q000737:chemistry* / Q000382:microbiology; D007194:India / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D022603:Shiga Toxins / Q000096:biosynthesis / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
chemical_list: D022603:Shiga Toxins