Generation of L7-tTA knock-in mice.
We generated a versatile mouse line, L7-tTA knock-in mouse, in which tetracycline-responsive transcriptional activator (tTA) gene was introduced into exon 2 of L7 locus. Since L7 is specifically expressed in cerebellar Purkinje cells, we expected Purkinje cell-restricted expression of tTA gene in the knock-in mice. In situ hybridization analysis exhibited that tTA mRNAs in those mice were expressed only in Purkinje cells. Introduction of transgene consisting of tetracycline-responsive element that is a binding site for tTA and ideal cDNAs into L7-tTA knock-in mouse would result in specific expression of cDNA encoding proteins in Purkinje cells and its expression could be controlled by doxycycline administration. L7-tTA knock-in mice would provide us with opportunity to elucidate the role of specific genes in cerebellar Purkinje cells.
Echigo
Ruka
R
Division of Molecular Genetics, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
Nakao
Kazuki
K
Fukaya
Masahiro
M
Watanabe
Masahiko
M
Aiba
Atsu
A
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D055879:Gene Knock-In Techniques; D005822:Genetic Vectors; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics*; D017403:In Situ Hybridization; D051379:Mice; D009479:Neuropeptides / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011689:Purkinje Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*
D020662:Guanine Nucleotide Exchange Factors; D009479:Neuropeptides; C104971:Pcp2 protein, mouse; D012333:RNA, Messenger; D015534:Trans-Activators