title: Coupled relaxations at the protein-water interface in the picosecond time scale.
abstract: The spectral behaviour of a protein and its hydration water has been investigated through neutron scattering. The availability of both hydrogenated and perdeuterated samples of maltose-binding protein (MBP) allowed us to directly measure with great accuracy the signal from the protein and the hydration water alone. Both the spectra of the MBP and its hydration water show two distinct relaxations, a behaviour that is reminiscent of glassy systems. The two components have been described using a phenomenological model that includes two Cole-Davidson functions. In MBP and its hydration water, the two relaxations take place with similar average characteristic times of approximately 10 and 0.2 ps. The common time scales of these relaxations suggest that they may be a preferential route to couple the dynamics of the water hydrogen-bond network around the protein surface with that of protein fluctuations.
authors.affiliation: Dipartimento di Fisica, Università degli Studi di Perugia, Via A. Pascoli, I-06123 Perugia, Italy. alessandro.paciaroni@fisica.unipg.it
authors.forename: A
authors.initials: A
authors.lastname: Paciaroni
authors.forename: E
authors.initials: E
authors.lastname: Cornicchi
authors.forename: M
authors.initials: M
authors.lastname: Marconi
authors.forename: A
authors.initials: A
authors.lastname: Orecchini
authors.forename: C
authors.initials: C
authors.lastname: Petrillo
authors.forename: M
authors.initials: M
authors.lastname: Haertlein
authors.forename: M
authors.initials: M
authors.lastname: Moulin
authors.forename: F
authors.initials: F
authors.lastname: Sacchetti
mesh_terms: D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D007700:Kinetics; D058619:Maltose-Binding Proteins; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D033363:Neutron Diffraction / Q000379:methods*; D011485:Protein Binding; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D046912:Multiprotein Complexes; D014867:Water