title: Separation of proteins using a novel two-depth miniaturized free-flow electrophoresis device with multiple outlet fractionation channels.
abstract: A novel free-flow electrophoresis glass chip design with two-depth etched structures for the separation and fractionation of proteins is presented. The microfluidic structures etched in two depths enhance the flow characteristics inside the miniaturized device. A novel nine-port outlet interface enables the fractionation of the separated analytes. The separation and focussing of a protein sample mixture demonstrated the ability of the new chip.
authors.affiliation: ISAS - Institute for Analytical Sciences, 44139 Dortmund, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Becker
authors.forename: Ulrich
authors.initials: U
authors.lastname: Marggraf
authors.forename: Dirk
authors.initials: D
authors.lastname: Janasek
mesh_terms: D000818:Animals; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005898:Glass; D006736:Horses; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008855:Microscopy, Electron, Scanning; D008904:Miniaturization; D009211:Myoglobin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D013499:Surface Properties; D014361:Trypsin Inhibitors / Q000302:isolation & purification
chemical_list: D009211:Myoglobin; D011506:Proteins; D014361:Trypsin Inhibitors