[Phylogenetic diversity of endophytic endospore-forming bacteria isolated from Cinnamomum longepaniculatum N. Chao ex H. W. Li].
OBJECTIVE
In order to study the diversity of endophytic endospore-forming bacteria in Cinnamomum longepaniculatum.


METHODS
We took modified nutrient agar medium for isolation and cultivation and analyzed the 16S rRNA gene sequences of the isolates.


RESULTS
Forty non-redundant endospore-forming bacterial isolates were ascertained, which accounted for 38.1% of all the endophytic bacterial isolates. Of them, 24 isolates were from roots, 7 from stems and 9 from leaves. Phylogenetic analysis based on 16S rRNA gene sequences showed that 35 of them belonged to 16 species of the genera Bacillus, Lysinibacillus and Paenibacillus, and 5 isolates with < 97% sequence similarities to their closely related members were presumed to be potential novel species.


CONCLUSIONS
The results showed that the cultivable endospore-forming bacteria diversity was abundant and there were some potential novel strains in Cinnamomum longepaniculatum. The microflora of endophytic endospore-forming bacteria in individuals of C. longepaniculatum showed that some bacteria distributed in different organs, but the others were organ-specific bacteria.
Wang
Tao
T
Key Laboratory of Fermentation Resources and Application at Universities of Sichuan Province, Yibin University, Yibin 644000, China. asdfgwt@yahoo.com.cn
You
Ling
L
Cui
Xiaolong
X
Tian
Wen
W
Huang
Naiyao
N
Li
Xinglong
X
D044822:Biodiversity; D027424:Cinnamomum / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004725:Endospore-Forming Bacteria / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S