[Study on HPLC fingerprint of Trachelospermum jasminoides].
OBJECTIVE
To establish HPLC fingerprint for identification and evaluation of the quality of Trachelospermum jasminoides.


METHODS
The analysis was performed on a ZORBAX Eclipse XDB-C18 analytical column (4.6 mm x 150 mm, 5 microm) with H2O (A) and methanol (B) as mobile phases in gradient mode. The elutin conditions were 0-15 min, changed from 10% B to 30% B; 15-40 min, to 40% B; 40-60 min, to 60% B. The column temperature was set at 30 degrees C and the flow rate was 0.8 mL x min(-1) with the detection wavelength of 230 nm.


RESULTS
The HPLC chromatographic fingerprint of T. jasminoides, showing 19 characteristic peaks, was established from 14 lots of T. jasminoides. The similarity of every lot of T. jasminoides was calculated as good in general, the similarity of 10 lots was above 0.9 and the other 4 lots had low similarity.


CONCLUSIONS
The chromatographic fingerprint of T. jasminoides with high characteristics and specificity can be used to control its quality.
Liu
Yiquan
Y
School of Pharmaceutical and Technology, Tianjin University, Tianjin 300072, China.
Yu
Nengjiang
N
Yang
Xuedong
X
Zhao
Yimin
Y
D029062:Apocynaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D011786:Quality Control
D004365:Drugs, Chinese Herbal