title: Size distribution of native cytosolic proteins of Thermoplasma acidophilum.
abstract: We used molecular sieve chromatography in combination with LC-MS/MS to identify protein complexes that can serve as templates in the template matching procedures of visual proteomics approaches. By this method the sample complexity was lowered sufficiently to identify 464 proteins and - on the basis of size distribution and bioinformatics analysis - 189 of them could be assigned as subunits of macromolecular complexes over the size of 300 kDa. From these we purified six stable complexes of Thermoplasma acidophilum whose size and subunit composition - analyzed by electron microscopy and MALDI-TOF-MS, respectively - verified the accuracy of our method.
authors.affiliation: Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.forename: Noriko
authors.initials: N
authors.lastname: Tamura
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tamura
authors.forename: Roland Wilhelm
authors.initials: RW
authors.lastname: Knispel
authors.forename: Thomas
authors.initials: T
authors.lastname: Hrabe
authors.forename: Christine
authors.initials: C
authors.lastname: Kofler
authors.forename: Stephan
authors.initials: S
authors.lastname: Nickell
authors.forename: István
authors.initials: I
authors.lastname: Nagy
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D003600:Cytosol / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D013822:Thermoplasma / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins