Cloning of Aspergillus oryzae Aovps5 gene, homologous to vacuolar protein sorting associated gene VPS5 and construction of the disruptant.
Aovps5 gene was isolated from Aspergillus oryzae as a homologue to S. cerevisiae VPS5 gene which encodes a polypeptide consisting of 451 amino acids that is nearly 32% homologous to Vps5p. Three Aovps5 gene disruptants were generated and they showed higher activity of tripeptidyl peptidase, which is mainly detected in vacuoles, in their culture medium. Higher amount of nitrogenous constituent was found in the filtrate of wheat gluten degraded by addition of culture medium of these disruptants than that of wild type strain. These results suggest that disruption of Aovps5 may contribute to production of fermented foods.
Tokunaga
Chikara
C
Kyowa Hakko Food Specialties Co., Ltd., Tokyo Marketing Center 34-2, Hakozaki-Cho, Nihonbashi Chuo-ku, Tokyo 103-0015, Japan. ctoku@rondo.plala.or.jp
Saitoh
Chiaki
C
Kitamoto
Katsuhiko
K
D000626:Aminopeptidases; D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D005516:Food Microbiology; D017353:Gene Deletion; D005983:Glutens / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000235:genetics*; D033921:Vesicular Transport Proteins
D029701:Saccharomyces cerevisiae Proteins; C106932:VPS5 protein, S cerevisiae; D033921:Vesicular Transport Proteins; D005983:Glutens; D010450:Endopeptidases; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases