A HindIII polymorphism of fibronectin gene is associated with nephrolithiasis.
OBJECTIVE
To evaluate the association between fibronectin gene (FN1) polymorphisms and calcium oxalate nephrolithiasis as a genetic risk factor.


METHODS
Genomic DNA of 143 patients with calcium oxalate nephrolithiasis and 154 healthy controls were screened for polymorphisms (HaeIII b, MspI, and HindIII) of the FN1 gene, using polymerase chain reaction-restriction fragments length polymorphism method. Allele and genotype frequencies were compared between the groups.


RESULTS
Although the observed differences between distribution of genotypes of AA, AB, and BB (for HaeIII b), as well as CC, CD, and DD (MspI) were not significant, FF genotype for HindIII showed significant difference when compared with both EF and EE + EF genotype (P = .00202 and P = .00203, respectively).


CONCLUSIONS
The results of our study revealed that HindIII polymorphism of the FN1 gene is highly associated with calcium oxalate stone disease. This association makes FN a good candidate for further studies about the etiology of stone disease, and in the future it could be a candidate marker for evaluating the genetic risks in patients with nephrolithiasis.
Onaran
Metin
M
Department of Urology, School of Medicine, Gazi University, Ankara, Turkey. monaran@gazi.edu
Yilmaz
Akin
A
Sen
Ilker
I
Ergun
Mehmet Ali
MA
Camtosun
Ahmet
A
Küpeli
Bora
B
Menevşe
Sevda
S
Bozkirli
Ibrahim
I
D000328:Adult; D000368:Aged; D002129:Calcium Oxalate / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000235:genetics; D019003:Deoxyribonuclease HpaII / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D053040:Nephrolithiasis / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D055815:Young Adult
D005353:Fibronectins; D002129:Calcium Oxalate; D015247:Deoxyribonuclease HindIII; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases