1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I.
Sticholysin I is an actinoporin, a pore forming toxin, of 176 aminoacids produced by the sea anemone Stichodactyla heliantus. Isotopically labelled (13)C/(15)N recombinant protein was produced in E. coli. Here we report the complete NMR (15)N, (13)C and (1)H chemical shifts assignments of Stn I at pH 4.0 and 25 degrees C (BMRB No. 15927).
Castrillo
Inés
I
Departamento de Espectroscopía y Estructura Molecular, Instituto de Química Física Rocasolano, CSIC, Serrano 119, 28006, Madrid, Spain.
Alegre-Cebollada
Jorge
J
del Pozo
Alvaro Martínez
AM
Gavilanes
José G
JG
Santoro
Jorge
J
Bruix
Marta
M
D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry*; D011522:Protons; D012615:Sea Anemones / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009930:Organic Chemicals; D052899:Pore Forming Cytotoxic Proteins; D011522:Protons; C121348:stycholysin I