The removal of fluorescence in sequencing-by-synthesis.
Fluorescent oligonucleotides provide useful way to study DNA during the sequencing-by-synthesis process. They allow researchers to determine the sequence of a short DNA strand. Fluorescence created by these chemicals must be removed after a few based pairs are read in order to continue reading the DNA sequence. Researchers have developed a variety of methods to solve this problem. This paper reviews the development of fluorescence removal in DNA sequencing and summarizes several methods of removing the fluorescence.
Gao
Li
L
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Lu
Zuhong
Z
D004247:DNA / Q000096:biosynthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009578:Nitrobenzenes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D010782:Photolysis; D017422:Sequence Analysis, DNA / Q000379:methods*
D005456:Fluorescent Dyes; D009578:Nitrobenzenes; D009841:Oligonucleotides; D004247:DNA