[The effects of shRNA-CXCR4 on breast cancer cells migration and invasion].
OBJECTIVE
To study the effect of shRNA-CXCR4 on human breast cancer cells migration and invasion.


METHODS
Through knockdown CXCR4 by shRNA, the CXCR4 mRNA expression and protein expression were examined with RT-PCR and Western blot separately. The matrigel penetrating ability of human breast cancer cells was examined in vitro experiments.


RESULTS
The CXCR4 mRNA express and its protein express decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells (P<0.05). The invasive ability of human breast cancer cells was significantly decreased (P<0.05).


CONCLUSIONS
through knockdown CXCR4 by shRNA, the invasive ability of human breast cancer cells can be inhibited obviously.
Li
Hong-yuan
HY
The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Ren
Guo-sheng
GS
Tan
Jin-xiang
JX
D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000378:metabolism
C527276:CXCR4 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019718:Receptors, CXCR4