title: [GST genes expression as prognostic factor in papillary thyroid cancer].
abstract: OBJECTIVE
  Analyze the relationship between the AMES classification and molecular factors from Glutation-S-Transferase System, specifically the GSTT1 and GSTM1 in patients with well differentiated thyroid cancer.
  
  
  METHODS
  Samples of thyroid tissue of 66 patients with papillary thyroid carcinoma were obtained (53 women and 13 men). Patients were divided in two groups (high and low risk) according to the AMES classification. In each group, presence of the null genotype of both GST enzymes system was studied. These results were compared with the AMES classification. Samples were obtained in the operating room immediately after thyroidectomy, placed in cryotubes, immersed in liquid nitrogen and stored in a freezer at -80 masculineC. DNA of this enzymes was extracted by the fenol-cloroformium method.
  
  
  RESULTS
  There were 17 high risk patients and 49 low risk patients. The null genotype of the high risk group was 5.8% and in the other group was 6.1%.
  
  
  CONCLUSIONS
  There was no relationship between absence of genes GSTT1 and GSTM1 and prognosis of the papillary thyroid carcinoma when compared to the AMES classifications.
authors.affiliation: Departamento de Cirurgia, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, SP. dr.goncalves@uol.com.br
authors.forename: Antonio Jose
authors.initials: AJ
authors.lastname: Gonçalves
authors.forename: Osmar
authors.initials: O
authors.lastname: Monte
authors.forename: Eliane Cristina
authors.initials: EC
authors.lastname: Morari
authors.forename: Laura Sterian
authors.initials: LS
authors.lastname: Ward
authors.forename: Diana Shimoda
authors.initials: DS
authors.lastname: Nakasako
authors.forename: Juliana
authors.initials: J
authors.lastname: Nieto
authors.forename: Marianne Yumi
authors.initials: MY
authors.lastname: Nakai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002291:Carcinoma, Papillary / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011446:Prospective Studies; D012307:Risk Factors; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: D005982:Glutathione Transferase; C413545:glutathione S-transferase T1