Methods for mucin analysis: a comparative study.
The aim was to compare five techniques commonly used to quantify mucin concentrations in ileal digesta collected from three growing pigs that had been fed a diet in which the sole protein was casein. Ileal mucin output was estimated by the periodic acid-Schiff, ethanol precipitation, and phenol-sulfuric acid methods as 25.1, 19.3, and 20.7 g kg-1 of dry matter intake (DMI), respectively. The mucin concentration estimated from sialic acid was only 5.9 g kg-1 of DMI. On the basis of the concentrations of the hexosamines N-acetylglucosamine and N-acetylgalactosamine, mucin output was estimated as 44.9 g kg-1 pf DMI. Of the five assays studied, the ethanol precipitation, periodic acid-Schiff, phenol-sulfuric acid, and sialic acid methods may considerably underestimate mucin in the digesta, which calls into question the accuracy of all of these approaches. In contrast, the gas chromatography method for the determination of hexosamines gave more information on the type and state of the mucin present.
Miner-Williams
Warren
W
Riddet Institute, Massey University, Palmerston North, New Zealand.
Moughan
Paul J
PJ
Fuller
Malcolm F
MF
D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000378:metabolism; D002849:Chromatography, Gas; D003124:Colorimetry; D004032:Diet; D004044:Dietary Proteins / Q000008:administration & dosage; D004063:Digestion; D005188:False Negative Reactions; D007082:Ileum / Q000378:metabolism; D009077:Mucins / Q000032:analysis* / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis; D013552:Swine
D000596:Amino Acids; D002364:Caseins; D004044:Dietary Proteins; D009077:Mucins; D012794:Sialic Acids; D000116:Acetylgalactosamine; D000117:Acetylglucosamine