title: Separation and determination of coumarins in Fructus cnidii extracts by pressurized capillary electrochromatography using a packed column with a monolithic outlet frit.
abstract: The pressurized capillary electrochromatography (pCEC) was utilized for the separation and determination of coumarins in Fructus cnidii extracts from 12 different regions. After a thorough study of analytical parameters such as acetonitrile content of the mobile phase, the concentration and pH of the buffer, and the applied voltage, a methodology was proposed to separate and determine six coumarins of F. cnidii extracts in less than 15 min. The experiments were performed in an in-house packed column with a monolithic outlet frit under the optimal conditions: pH 4.0 ammonium acetate buffer at 10 mM containing 50% acetonitrile at -6kV applied voltage. The calibration curves were linear in the range of 10.0-100.0 microg/mL for bergapten, 20.0-200.0 microg/mL for imperatorin, 5.0-400.0 microg/mL for osthole, 10.0-100.0 microg/mL for 2'-acetylangelicin, 10.0-200.0 microg/mL for oroselone, and 10.0-200.0 microg/mL for O-acetylcolumbianetin. The correlation coefficients were between 0.9967 and 0.9995. With this pCEC system, fingerprints of F. cnidii extracts were preliminarily established to distinguish three types of coumarins by characteristic peaks, and the quality of various sources of raw materials was evaluated by determining the contents of six coumarins.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China.
authors.forename: Danxia
authors.initials: D
authors.lastname: Chen
authors.forename: Jiajing
authors.initials: J
authors.lastname: Wang
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.forename: Yutian
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D019661:Apiaceae / Q000378:metabolism; D053801:Capillary Electrochromatography / Q000379:methods*; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002681:China; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008516:Medicine, Chinese Traditional; D008956:Models, Chemical; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D000085:Acetates; D000097:Acetonitriles; D003374:Coumarins; D010936:Plant Extracts; C030123:coumarin; C018824:ammonium acetate; C032159:acetonitrile