title: Consensus sequence L/PKSSLL mimics crucial epitope on Loop III of Taiwan cobra cardiotoxin.
abstract: Phage display is effective in screening peptides that mimic venom's neutralizing epitopes. A phage display cyclized heptapeptide library (C7C library) was panned with purified divalent antivenin IgG, which neutralizes Naja naja atra venom (NAV) and Bungarus multicinctus venom (BMV). The selected heptapeptide sequences were aligned with known protein sequences of NAV and BMV in GenBank. One of the four consensus sequences, L/PKSSLL, mimicked the crucial epitope on Loop III of Taiwan cobra cardiotoxin that is associated with the venom's lethal potency. In dot blot analysis, several clones showed varying reactivities for NAV monovalent antivenin and lesser cross-reactions with BMV monovalent antivenin. The KSSLLRN-carrying phage occurred four times in selected clones and showed the strongest reactivity to NAV monovalent antivenin. Furthermore, the QDSLLPS-carrying phage also presented significant dot blot signal, indicating that the SLL sequence shared by these two clones may be a crucial antibody-binding site.
authors.affiliation: Institute of Biotechnology, National University of Kaohsiung, Kaohsiung 811, Taiwan.
authors.forename: Ping-Chieh
authors.initials: PC
authors.lastname: Wang
authors.forename: Kah-Sin
authors.initials: KS
authors.lastname: Loh
authors.forename: Shih-Ting
authors.initials: ST
authors.lastname: Lin
authors.forename: Tzu-Ling
authors.initials: TL
authors.lastname: Chien
authors.forename: Jen-Ron
authors.initials: JR
authors.lastname: Chiang
authors.forename: Wen-Chin
authors.initials: WC
authors.lastname: Hsieh
authors.forename: Bor-Lin
authors.initials: BL
authors.lastname: Miao
authors.forename: Cheng-Fu
authors.initials: CF
authors.lastname: Su
authors.forename: Wen-Jen
authors.initials: WJ
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D004179:Cobra Cardiotoxin Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D016384:Consensus Sequence; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019151:Peptide Library; D017433:Protein Structure, Secondary
chemical_list: D000906:Antibodies; D004179:Cobra Cardiotoxin Proteins; D000939:Epitopes; D019151:Peptide Library