title: Characterization of zebrafish larval inflammatory macrophages.
abstract: Zebrafish have emerged as a powerful model system to study leukocyte recruitment and inflammation. Here we characterize the morphology and function of inflammatory macrophages in zebrafish larvae. These macrophages can be distinguished from neutrophils by immunolabeling of L-Plastin without MPO co-expression and by an elongated morphology. Live imaging of transgenic zMPO:GFP larvae demonstrate that GFP(lo) macrophages migrate to wounds by extension of thin pseudopods and carry out phagocytosis of tissue debris, and FACS analysis of leukocyte markers indicates expression of CSF1R in these macrophages. These findings identify distinct functional and morphological characteristics of inflammatory macrophages in zebrafish larvae.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, USA.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Mathias
authors.forename: M Ernest
authors.initials: ME
authors.lastname: Dodd
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Walters
authors.forename: Sa Kan
authors.initials: SK
authors.lastname: Yoo
authors.forename: Erik A
authors.initials: EA
authors.lastname: Ranheim
authors.forename: Anna
authors.initials: A
authors.lastname: Huttenlocher
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement / Q000276:immunology; D002469:Cell Separation; D005434:Flow Cytometry; D007249:Inflammation; D007814:Larva / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis; D011554:Pseudopodia / Q000276:immunology; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015027:Zebrafish / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; C026742:plastin; D009195:Peroxidase; D016186:Receptor, Macrophage Colony-Stimulating Factor