Effect of polymer chemistry and fabrication method on protein release and stability from polyanhydride microspheres.
The release kinetics and stability of ovalbumin encapsulated into polyanhydride microspheres with varying chemistries were studied. Polymers based on the anhydride monomers sebacic acid (SA), 1,6-bis(p-carboxyphenoxy)hexane (CPH), and 1,8-bis (p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) were utilized. Microspheres were fabricated using two non-aqueous methods: a solid/oil/oil double emulsion technique and cryogenic atomization. The studies showed that the two fabrication methods did not significantly affect the release kinetics of ovalbumin, even though the burst release of the protein was a function of the fabrication method and the polymer chemistry. Antigenic stability of ovalbumin released from microspheres prepared by cryogenic atomization was studied by western blot analysis. These studies indicate that the amphiphilic CPTEG:CPH polyanhydrides preserved protein structure and enhanced protein stability by preserving the immunological epitopes of released protein.
Lopac
Senja K
SK
Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011.
Torres
Maria P
MP
Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011.
Wilson-Welder
Jennifer H
JH
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011.
Wannemuehler
Michael J
MJ
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011.
Narasimhan
Balaji
B
Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011.
D001672:Biocompatible Materials / Q000737:chemistry; D015153:Blotting, Western; D003652:Decanoic Acids / Q000737:chemistry; D003998:Dicarboxylic Acids / Q000737:chemistry; D004337:Drug Carriers; D004355:Drug Stability; D006586:Hexanes / Q000737:chemistry; D007700:Kinetics; D008863:Microspheres; D010047:Ovalbumin / Q000737:chemistry; D010316:Particle Size; D049388:Polyanhydrides / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry; D012995:Solubility
C120991:1,6-bis(carboxyphenoxy)hexane; D001672:Biocompatible Materials; D003652:Decanoic Acids; D003998:Dicarboxylic Acids; D004337:Drug Carriers; D006586:Hexanes; D049388:Polyanhydrides; D011506:Proteins; C546712:poly(1,8-bis(4-carboxyphenoxy)-3,6-dioxaoctane); D011092:Polyethylene Glycols; D010047:Ovalbumin; C011107:sebacic acid