The use of injectable spherically symmetric cell aggregates self-assembled in a thermo-responsive hydrogel for enhanced cell transplantation.
Typical cell transplantation techniques involve the administration of dissociated cells directly injected into muscular tissues; however, retention of the transplanted cells at the sites of the cell graft is frequently limited. An approach, using spherically symmetric aggregates of cells with a relatively uniform size self-assembled in a thermo-responsive methylcellulose hydrogel system, is reported in the study. The obtained cell aggregates preserved their endogenous extracellular matrices (ECM) and intercellular junctions because no proteolytic enzyme was used when harvesting the cell aggregates. Most of the cells within aggregates (with a radius of approximately 100 microm) were viable as indicated by the live/dead staining assay. After injection through a needle, the cell aggregates remained intact and the cells retained their activity upon transferring to another growth surface. The cell aggregates obtained under sterile conditions were transplanted into the skeletal muscle of rats via local injection. The dissociated cells were used as a control. It was found that the cell aggregates can provide an adequate physical size to entrap into the muscular interstices and offer a favorable ECM environment to enhance retention of the transplanted cells at the sites of the cell graft. These results indicated that the spherically symmetric cell aggregates developed in the study may serve as a cell delivery vehicle for therapeutic applications.
Lee
Wen-Yu
WY
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, ROC.
Chang
Yu-Hsiang
YH
Yeh
Yi-Chun
YC
Chen
Chun-Hung
CH
Lin
Kurt M
KM
Huang
Chieh-Cheng
CC
Chang
Yen
Y
Sung
Hsing-Wen
HW
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D008422:Materials Testing; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000601:surgery*; D051381:Rats; D011917:Rats, Inbred Lew; D013102:Spherocytes; D013696:Temperature
D001672:Biocompatible Materials; D020100:Hydrogels