title: Separation of chitooligosaccharides and the potent effects on gene expression of cell surface receptor CR3.
abstract: Chitooligosaccharides were prepared through hydrolysis of colloidal chitosan by enzyme source from Aspergillusfumigatus BSF114. Chitosan pentamer (COS5) and chitosan hexamer (COS6) were isolated and purified from COS by the ultra-filtration, nano-filtration, ethanol precipitation and the CM-Sephadex C-25 column. COS5 consisted of (GlcN)4 (59.84%) and (GLcN)5 (40.16%). COS6, however, mainly consisted of (GLcN)6 (93.11%) and (GLcN)(5) (6.89%). Effects of COS5 and COS6 in vivo and in vitro on gene expression of cell surface CR3 receptor were investigated by relatively quantitative RT-PCR and ELISA. The results showed that the expression of CR3 mRNA could be promoted by both COS5 and COS6. The promotion effect caused by COS6 was greater than that of COS5.
authors.affiliation: College of Life and Environment Science, Shanghai Normal University, Shanghai, China.
authors.forename: Xinlin
authors.initials: X
authors.lastname: Wei
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Xiao
authors.forename: Wenshui
authors.initials: W
authors.lastname: Xia
mesh_terms: D000818:Animals; D048271:Chitosan / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D005944:Glucosamine / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000235:genetics*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
chemical_list: D016177:Macrophage-1 Antigen; D009844:Oligosaccharides; D048271:Chitosan; D005944:Glucosamine