[Effect of p21-activated kinase-1 on the invasiveness of colorectal carcinoma cells in vitro].
OBJECTIVE
To observe the effect of p21-activated kinase-1 (PAK1) gene transfection on the invasiveness of human colorectal carcinoma SW480 cells in vitro.


METHODS
SW480 cells in routine culture were transfected with the recombinant plasmid EGFP-C1/PAK1 via Lipofectamine(TM) 2000. The expression of PAK1 protein in SW480 cells was detected using Western blotting, and the changes of the invasiveness of SW480 cells were evaluated using Boyden chamber invasion assay.


RESULTS
Forty-eight hours after transfection with pEGFP-C1/ PAK1, the PAK1 protein expression increased significantly in comparison with those in negative and vector control groups. The invasiveness of the SW480 cells was significantly enhanced after the transfection.


CONCLUSIONS
The PAK1 gene transfection can increase the expression of PAK1 in SW480 cells and enhance the invasiveness of the cells. PAK1 can be associated with the invasiveness and metastasis of colorectal carcinoma cells.
Wu
Jin-Bao
JB
Institute of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.: jqbwu@163.com
Han
Yu-Jing
YJ
Nan
Qing-Zhen
QZ
Zhang
Zhen-Shu
ZS
Zhang
Hong-Quan
HQ
Song
Yu-Gang
YG
D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010957:Plasmids; D014162:Transfection; D054462:p21-Activated Kinases / Q000235:genetics*
C515903:PAK1 protein, human; D054462:p21-Activated Kinases