title: Effects of pretransfusion warming of platelets to 35 degrees C on posttransfusion platelet viability.
abstract: BACKGROUND
  Three of four prior studies suggested that warming platelets (PLTs) to 37 degrees C before transfusion into patients with thrombocytopenia gave improved corrected PLT count increments.
  
  
  METHODS
  Eighteen normal subjects had apheresis PLTs collected that were stored at 22 degrees C for 5 days in two storage bags. One bag of PLTs was warmed to 35 degrees C before infusion, and one remained at 22 degrees C. Three different methods of warming the donor's autologous PLTs before reinfusion were evaluated: warming PLTs to 35 degrees C for 10 or 60 minutes followed by radiolabeling or radiolabeling the PLTs followed by warming to 35 degrees C for 60 minutes. In the first two methods, the warmed PLTs would have returned to 22 degrees C before infusion, and in the third, the PLTs would still be warm when injected. The paired test and control PLTs were radiolabeled with either (111)In or (51)Cr to determine posttransfusion PLT recoveries and survivals. PLT morphology score, pH, hypotonic shock response, extent of shape change, and annexin V binding were determined just before transfusion.
  
  
  RESULTS
  There were no differences in posttransfusion autologous radiolabeled PLT recoveries and survivals or in the in vitro measurements for the PLTs maintained at 22 degrees C versus those warmed to 35 degrees C using any of the three methods of PLT warming before infusion.
  
  
  CONCLUSIONS
  Based on these 5-day-stored autologous radiolabeled PLT recovery and survival measurements, there is no evidence that warming PLTs to 35 degrees C before infusion improves postinfusion PLT viability.
authors.affiliation: Platelet Transfusion Research, Puget Sound Blood Center, 921 Terry Avenue, Seattle, WA 98104-1256, USA. sjslichter@psbc.org
authors.forename: Sherrill J
authors.initials: SJ
authors.lastname: Slichter
authors.forename: Todd
authors.initials: T
authors.lastname: Christoffel
authors.forename: Jill
authors.initials: J
authors.lastname: Corson
authors.forename: Mary Kay
authors.initials: MK
authors.lastname: Jones
authors.forename: Esther
authors.initials: E
authors.lastname: Pellham
authors.forename: Doug
authors.initials: D
authors.lastname: Bolgiano
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000009:adverse effects*; D002470:Cell Survival / Q000502:physiology*; D006801:Humans; D017713:Platelet Transfusion; D013696:Temperature