title: [Effects of different oxygen flow rates on myocardial ischemia-reperfusion injury in rabbits].
abstract: OBJECTIVE
  To explore the effects of different oxygen flow rates during cardiopulmonary bypass (CPB) on myocardial ischemia-reperfusion (IR) injury in rabbits.
  
  
  METHODS
  Thirty rabbits were randomized equally into groups A, B and C to receive controlled oxygen reperfusion at low, normal and high flow rates (25, 50, and 80 ml.kg(-1).min(-1), respectively). Serum concentration of CK-MB and cTnT were tested by ELISA before the operation (T0) and after 30 min (T1), 2 h (T2), 12 h (T3) and 24 h (T4) of reperfusion. W/D, SOD and MDA of the myocardium were determined before and at 60 min after reperfusion. The ultrastructural alterations of the myocardium were observed.
  
  
  RESULTS
  Serum concentration of CK-MB and cTnT in the 3 groups increased significantly after the operation, and their levels were the lowest in group A (P<0.05). W/D and MDA in the myocardium was also the lowest, while SOD the highest in group A (P<0.05). Ultrastructural pathologies were found in all the 3 groups, but relatively mild in group A.
  
  
  CONCLUSIONS
  Low oxygen flow rate during controlled reperfusion may protect the myocardium from IR injury in rabbits.
authors.affiliation: Department of Cardiothoracic Surgery, Third Xiangya Hospital of Central South University, Changsha 410013, China. gongwei21star@sohu.com
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Liu
authors.forename: Long-Yu
authors.initials: LY
authors.lastname: Jin
authors.forename: Dian-Jun
authors.initials: DJ
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Hong
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D010100:Oxygen / Q000008:administration & dosage*; D011817:Rabbits; D015424:Reperfusion / Q000379:methods*
chemical_list: D010100:Oxygen