A high-resolution radiation hybrid map of sheep chromosome X and comparison with human and cattle.
A radiation hybrid (RH) map of sheep X chromosome (Ovisaries; OARX) containing 146 physically anchored loci was generated in this study, providing information for comparative X chromosome analysis between the maps of sheep, human, and cattle. Primers typed on the USUoRH5000 ovine whole-genome radiation hybrid panel were designed from sequences predicted to be on the ovine X chromosome, based on comparative mapping within the virtual sheep genome browser (v1.2). The resulting RH map for the ovine X chromosome consists of 4 linkage groups composed of 76 BAC end sequences (BES), 28 gene loci that were confirmed within ovine BAC clones in the CHORI-243 ovine BAC library, 28 additional gene loci from the ovine comparative map and 14 polymorphic sequence tagged sites (STS) from the OARX linkage map. This first-generation RH map of OARX contributes to the expansion of a comprehensive ovine genome map for sheep and provides evidence of rearrangements in loci order compared to the human and cattle orders.
Goldammer
T
T
Forschungsbereich Molekularbiologie, Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Dummerstorf, Germany. tomgoldammer@fbn-dummerstorf.de
Brunner
R M
RM
Rebl
A
A
Wu
C H
CH
Nomura
K
K
Hadfield
T
T
Gill
C
C
Dalrymple
B P
BP
Womack
J E
JE
Cockett
N E
NE
D000818:Animals; D002417:Cattle / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats; D021101:Radiation Hybrid Mapping / Q000379:methods / Q000662:veterinary*; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity; D014960:X Chromosome / Q000235:genetics*