title: Tissue expression of manganese superoxide dismutase is a candidate prognostic marker for glioblastoma.
abstract: BACKGROUND
  Characterization of a rare subgroup of glioblastoma patients who survive for more than 3 years (long-term survival glioblastoma, LTSGBL, patients) may be helpful to identify prognostic factors.
  
  
  METHODS
  A molecular-profiling proteomic approach using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify prognostic factors associated with glioblastoma by comparing frozen tumor tissue from LTSGBL patients with matched samples from short-term survival glioblastoma (STSGBL) patients. Western blot (WB) analysis, reverse-transcriptase polymerase chain reaction (RT-PCR) and immmunohistochemical (IHC) staining were used for confirmation.
  
  
  RESULTS
  Among most candidate spots identified by 2-DE, lack of overexpression of manganese superoxide dismutase (MnSOD) in LTSGBL samples was consistently observed using WB and RT-PCR.
  
  
  CONCLUSIONS
  These results suggest that MnSOD expression level in tumor tissue is a candidate marker for the prognosis of glioblastoma patients.
authors.affiliation: Department of Neurosurgery, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Chul-Kee
authors.initials: CK
authors.lastname: Park
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Jung
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Moon
authors.forename: Young-Yim
authors.initials: YY
authors.lastname: Kim
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Sung-Hye
authors.initials: SH
authors.lastname: Park
authors.forename: Chae-Yong
authors.initials: CY
authors.lastname: Kim
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Paek
authors.forename: Dong Gyu
authors.initials: DG
authors.lastname: Kim
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Jung
authors.forename: Byung-Kyu
authors.initials: BK
authors.lastname: Cho
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000201:enzymology* / Q000401:mortality; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005909:Glioblastoma / Q000175:diagnosis / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase / Q000032:analysis*; D000099156:Superoxide Dismutase 2
chemical_list: D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2