title: Carcinoma cell-derived chemokines and their presence in oral fluid.
abstract: Chemokines are important in inflammation and in carcinogenesis. We hypothesized that besides oro-laryngeal cancer, oral inflammatory states, such as periodontitis, may also influence the chemokine profile of oral fluid. The aim of this study was to characterize the chemokine isoforms in the oral fluid of patients with periodontitis and in the oral fluid of patients with head and neck cancer. Using enzyme-linked immunosorbent assays (ELISA), it was found that the concentrations of CXCL8, CXCL10, and CCL14 were significantly elevated in the oral fluids of the cancer patients. However, periodontitis did not significantly alter the chemokine levels in oral fluid. Identification of chemokine isoforms by a proteomic approach using a newly developed three-step purification procedure was applied on the oral fluid of head and neck cancer and periodontitis patients and on the conditioned medium from carcinoma cells. Carcinoma cells produced predominantly intact CXCL1, CXCL2, CXCL8, and CCL2, whereas CXCL8 also appeared in a truncated, more active, form. Unfortunately, the chemokine concentrations in oral fluids were too low to allow full biochemical identification of the modified isoforms. However, the chemokine profile of head and neck cancer significantly changed after therapy, indicating that it is a useful parameter in clinical practice.
authors.affiliation: Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Belgium.
authors.forename: Katleen
authors.initials: K
authors.lastname: Michiels
authors.forename: Evemie
authors.initials: E
authors.lastname: Schutyser
authors.forename: René
authors.initials: R
authors.lastname: Conings
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Lenaerts
authors.forename: Willy
authors.initials: W
authors.lastname: Put
authors.forename: Sandra
authors.initials: S
authors.lastname: Nuyts
authors.forename: Pierre
authors.initials: P
authors.lastname: Delaere
authors.forename: Reinhilde
authors.initials: R
authors.lastname: Jacobs
authors.forename: Sofie
authors.initials: S
authors.lastname: Struyf
authors.forename: Paul
authors.initials: P
authors.lastname: Proost
authors.forename: Jo
authors.initials: J
authors.lastname: Van Damme
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000628:therapy; D018932:Chemokine CCL2 / Q000032:analysis; D054360:Chemokine CXCL1 / Q000032:analysis; D054357:Chemokine CXCL10 / Q000032:analysis; D054426:Chemokine CXCL2 / Q000032:analysis; D018925:Chemokines / Q000032:analysis*; D019742:Chemokines, CC / Q000032:analysis; D019743:Chemokines, CXC / Q000032:analysis; D055113:Chronic Periodontitis / Q000276:immunology / Q000628:therapy; D017077:Culture Media, Conditioned; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000276:immunology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000276:immunology* / Q000628:therapy; D020033:Protein Isoforms / Q000032:analysis; D011499:Protein Processing, Post-Translational; D020543:Proteome; D012463:Saliva / Q000276:immunology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*
chemical_list: C097720:CCL14 protein, human; C111792:CCL18 protein, human; C111835:CCL2 protein, human; C475829:CXCL1 protein, human; C114753:CXCL10 protein, human; C118056:CXCL14 protein, human; C499706:CXCL2 protein, human; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D054357:Chemokine CXCL10; D054426:Chemokine CXCL2; D018925:Chemokines; D019742:Chemokines, CC; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; D020033:Protein Isoforms; D020543:Proteome; D012471:Salivary Proteins and Peptides