title: Metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates in Tunisia.
abstract: This study was conducted to identify metallo-beta-lactamase (MBL) producers among a collection of 75 nonrepetitive carbapenem-resistant Pseudomonas aeruginosa isolates recovered between November 2003 and May 2007 at the University Hospital Sahloul, Sousse, Tunisia. Five isolates produced the MBL VIM-2. Those bla(VIM-2)-positive isolates were clonally related according to pulsed-field gel electrophoresis analysis. This carbapenemase was very likely chromosomally located and as a form of a gene cassette in a class 1 integron. This is the 1st report of spread of VIM-2 producers in Tunisia.
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Paris-Sud, 94275 K.-Bicêtre, France.
authors.forename: Wejdene
authors.initials: W
authors.lastname: Mansour
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Dalia
authors.initials: D
authors.lastname: Bettaieb
authors.forename: Olfa
authors.initials: O
authors.lastname: Bouallegue
authors.forename: Noureddine
authors.initials: N
authors.lastname: Boujaafar
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques / Q000379:methods; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D032023:Integrons; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D014416:Tunisia; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases