title: An amperometric D-amino acid biosensor prepared with a thermostable D-proline dehydrogenase and a carbon nanotube-ionic liquid gel.
abstract: Carbon nanotube (CNT) gel, which is composed of a mixture of single-wall CNT, an ionic liquid, and a thermostable D-proline dehydrogenase (D-Pro DH) immobilized electrode was utilized for the determination of D-amino acids (DAAs) in food samples. When a critical comparison with CNT, Ketjen Black (KB), and carbon powder (CP) was also carried out, the CNT/D-Pro DH immobilized electrode showed the highest sensitivity and the lowest detection limit of D-proline. In addition, the CNT/D-Pro DH immobilized electrode was applied to detection of DAAs in rice wine and vinegar samples. The concentrations of DAAs in rice wine and vinegar samples were 0.0210 +/- 0.0001 and 0.55 +/- 0.05 mmol L(-1), respectively.
authors.affiliation: Department of Chemical Science and Technology, Institute of Science and Tecnhology, The University of Tokushima, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tani
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Itoyama
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nishi
authors.forename: Chikahiro
authors.initials: C
authors.lastname: Wada
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Shoda
authors.forename: Takenori
authors.initials: T
authors.lastname: Satomura
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohshima
authors.forename: Yukako
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yabutani
authors.forename: Junko
authors.initials: J
authors.lastname: Motonaka
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D004563:Electrochemistry; D004566:Electrodes; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005782:Gels; D052578:Ionic Liquids / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012275:Oryza; D011394:Proline Oxidase / Q000378:metabolism*; D013499:Surface Properties; D013696:Temperature; D014920:Wine / Q000032:analysis
chemical_list: D000596:Amino Acids; D004800:Enzymes, Immobilized; D005782:Gels; D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D011394:Proline Oxidase; D019342:Acetic Acid