title: Surgically-induced bile duct injury is followed by early hepatic oxidative stress. A preliminary experimental study in rats.
abstract: OBJECTIVE
  To study in the rat whether oxidative stress appears early in a model of short-term cholestasis induced by bile duct ligation.
  
  
  METHODS
  Adult male rats underwent proximal bile duct ligation; controls underwent a sham operation. Animals were sacrificed at days 0, 1, 3, 7 and 10. Livers were removed for histological studies and biochemical analysis.
  
  
  RESULTS
  GSH and PSH levels declined earlier in mitochondria than cytosol. Data were analyzed for statistical significance by ANOVA analysis.
  
  
  CONCLUSIONS
  This study represents an experimental model which helps in the interpretation of the damage induced by bile duct injury in humans emphasizing an early detection of bile duct injury after surgery to ensure appropriate treatment and optimal patient outcome.
authors.affiliation: Section of General Surgery A. De Blasi Department for the Application in Surgery of Innovative Technologies, University of Bari, Bari, Italy.
authors.forename: Germana
authors.initials: G
authors.lastname: Lissidini
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Piccinni
authors.forename: Piero
authors.initials: P
authors.lastname: Portincasa
authors.forename: Ignazio
authors.initials: I
authors.lastname: Grattagliano
authors.forename: Angela
authors.initials: A
authors.lastname: Gurrado
authors.forename: Mario
authors.initials: M
authors.lastname: Testini
mesh_terms: D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D003135:Common Bile Duct; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D013438:Sulfhydryl Compounds; D005978:Glutathione