title: Pseudomonas caeni sp. nov., a denitrifying bacterium isolated from the sludge of an anaerobic ammonium-oxidizing bioreactor.
abstract: Two Gram-negative, rod-shaped, motile, aerobic bacterial strains designated HY-14(T) and HY-24 were isolated from the sludge of an anaerobic ammonium-oxidizing bioreactor. The strains could not grow with 5 % (w/v) NaCl, did not produce acid from d-glucose or utilize d-glucose, gluconate or citrate as a sole carbon source. Summed feature 3 and C(16 : 0) were the most abundant fatty acids; hydroxyl fatty acids C(12 : 0) 3-OH and C(10 : 0) 3-OH were also present. Fatty acid C(12 : 0) 2-OH was absent. The DNA G+C contents of strains HY-14(T) and HY-24 were 50.6+/-0.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains HY-14(T) and HY-24 formed a monophyletic clade within the genus Pseudomonas. The highest sequence similarities were to Pseudomonas pseudoalcaligenes DSM 50188(T) (95.9 %). On the basis of phenotypic and phylogenetic properties, strains HY-14(T) and HY-24 are proposed as a novel species of the genus Pseudomonas, for which the name Pseudomonas caeni sp. nov. is proposed. The type strain is HY-14(T) (=KCTC 22292(T)=CCTCC AB208156(T)).
authors.affiliation: Department of Microbiology, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Hui
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Shi
authors.forename: Jin-Huan
authors.initials: JH
authors.lastname: Shi
authors.forename: Zhe-Xue
authors.initials: ZX
authors.lastname: Quan
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009573:Nitrites; D000644:Quaternary Ammonium Compounds; D012336:RNA, Ribosomal, 16S; D012722:Sewage