[Effects of primary tumor excision on angiogenesis and pulmonary metastasis in osteosarcoma-bearing nude mice].
OBJECTIVE
To investigate the effects of primary tumor excision on osteosarcoma angiogenesis and pulmonary metastasis, and explore its possible mechanism and clinical significance.


METHODS
The tumor-bearing nude mice were constructed by injection of human osteosarcoma cell suspension, and divided into primary tumor resection, amputation and normal groups. The level of VEGF and endostatin was examined by ELISA assay. The hemoglobin content in Matrigel pellets was measured with HiCN method. Pulmonary metastasis was detected with dilated-squash technique and immunohistochemical staining.


RESULTS
The serum VEGF and endostatin level was significantly decreased in the primary tumor excision group compared with that in the normal and control groups after operation, but endostatin decreased more prominently [VEGF: (71.43 +/- 9.15) pg/ml vs. (115.81 +/- 4.38) pg/ml, (111.68 +/- 12.26) pg/ml, P < 0.01; ES: (40.77 +/- 5.41) ng/ml vs. (123.18 +/- 5.94) ng/ml, (128.06 +/- 4.52) ng/ml, P < 0.01]. The HB contents in Matrigel pellets increased in the primary tumor excision group compared with that in normal and control groups [(36.55 +/- 2.35) g/L vs. (16.84 +/- 1.15) g/L, (16.29 +/- 1.10) g/L, P < 0.01]. The rate of pulmonary metastasis in tumor excision group was much higher than that in the un-excised groups (80.0% vs. 40.0% and 35.0%, P < 0.05).


CONCLUSIONS
The excision of primary tumor can promote osteosarcoma angiogenesis and pulmonary metastasis, so anti-angiogenic treatment after operation has instructive clinical significance in preventing tumor metastasis.
Mei
Jiong
J
Department of Orthopaedics, Tongji Hospital of Tongji University, Shanghai 200065, China. meijiong@tongji.edu.cn
Ni
Ming
M
Chen
Yan-xi
YX
Yao
Bin
B
Gao
You-shui
YS
Yu
Xiu-mao
XM
Wang
Zhi-yuan
ZY
D020533:Angiogenesis Inhibitors / Q000097:blood; D000818:Animals; D001859:Bone Neoplasms / Q000097:blood / Q000473:pathology / Q000601:surgery*; D045744:Cell Line, Tumor; D043169:Endostatins / Q000097:blood; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000473:pathology*; D012516:Osteosarcoma / Q000097:blood / Q000473:pathology / Q000601:surgery*; D042442:Vascular Endothelial Growth Factors / Q000097:blood
D020533:Angiogenesis Inhibitors; D043169:Endostatins; D006454:Hemoglobins; D042442:Vascular Endothelial Growth Factors