title: Functionalized nanoparticles for bioanalysis by SERRS.
abstract: Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.
authors.affiliation: Department of Pure and Applied Chemistry, Centre for Molecular Nanometrology, WestCHEM, University of Strathclyde, Glasgow G1 1XL, UK. duncan.graham@strath.ac.uk
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
authors.forename: David
authors.initials: D
authors.lastname: Thompson
authors.forename: Fiona
authors.initials: F
authors.lastname: McKenzie
authors.forename: Robert
authors.initials: R
authors.lastname: Stokes
authors.forename: Colette
authors.initials: C
authors.lastname: Dalton
authors.forename: Ross
authors.initials: R
authors.lastname: Stevenson
authors.forename: Jim
authors.initials: J
authors.lastname: Alexander
authors.forename: Paul
authors.initials: P
authors.lastname: Garside
authors.forename: Emma
authors.initials: E
authors.lastname: McFarlane
mesh_terms: D007118:Immunoassay / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D020349:Surface Plasmon Resonance