[An experimental study of protective effects of ischemic preconditioning and oxymatrine on lung ischemia reperfusion injury].
OBJECTIVE
To investigate the protective effects of ischemic preconditioning (IPC) and oxymatrine (OMT) on lung ischemia reperfusion injury (LIRI) in rabbit.


METHODS
Animal models of LIRI in rabbit were used. Twenty four rabbits were randomly divided into 4 groups: Group sham (n=6), Group I/R (ischemic reperfusion, n=6), Group IPC (n=6), Group IPC plus OMT (n=6). Lung tissue samples were collected at 0 min after thoracotomy, 40 min after lung ischemia and 80 min, 120 min, 160 min after lung reperfusion. SOD (Superoxide Dismutase), MDA (malondialdehyde), Hsp90 alpha (Heat Shock Protein 90 alpha), AI (Apoptosis Index), W/D (Wet/Dry weight ratio) and histology of lung by light microscope were studied in each group.


RESULTS
SOD, MDA in Groups sham, I/R, IPC and IPC plus OMT were not significantly different at 0 min, 40 min (P>0.05). AI and W/D in Group I/R were significantly higher than that of Groups IPC and IPC plus OMT at 80 min after LIR (P<0.05, P<0.01), SOD activity and the expressions levels of Hsp90 alpha were significantly lower than that of Groups IPC and IPC plus OMT (P<0.05, P<0.01), and MDA, W/D, AI in Group IPC plus OMT were significantly lower than that of Group IPC (P<0.05), but the expressions levels of SOD and Hsp90 alpha were significantly higher than that of Group IPC (P<0.05). The lung tissue injury in Group IPC plus OMT was less than Groups IPC and I/R.


CONCLUSIONS
IPC and OMT can protect against LIRI in rabbits by decreasing the levels of MDA and increasing the expressions of SOD and Hsp90 alpha, and inhibiting the apoptosis of alveolar cells after LIRI.
Chen
Shi-feng
SF
Department of Thoracocardiac Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
Wang
He
H
D000470:Alkaloids / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D019194:Ischemic Preconditioning; D008168:Lung / Q000098:blood supply*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011807:Quinolizines / Q000494:pharmacology / Q000627:therapeutic use*; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D000093842:Matrines
D000470:Alkaloids; D018841:HSP90 Heat-Shock Proteins; D008315:Malondialdehyde; D011807:Quinolizines; D013482:Superoxide Dismutase; C037573:oxymatrine; D000093842:Matrines