Electron microscopy of helical filaments: rediscovering buried treasures in negative stain.
Although negative stain electron microscopy is a wonderfully simple way of directly visualizing protein complexes and other biological macromolecules, the images are not really comparable to those of objects seen in everyday life. The failure to appreciate this has recently led to an incorrect interpretation of RecA-family filament structures.
Egelman
Edward H
EH
Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908-0733, USA. egelman@virginia.edu
Amos
Linda A
LA
D004247:DNA / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D011938:Rec A Recombinases / Q000648:ultrastructure
D004268:DNA-Binding Proteins; D004247:DNA; D011938:Rec A Recombinases