Sigma-2 receptor expression in bovine papillomavirus-associated urinary bladder tumours.
The expression of sigma-2 receptors was investigated in nine urothelial tumours of the urinary bladder of cattle. Each tumour was associated with the presence of DNA of bovine papillomavirus type-2 (BPV-2) and expression of the E5 viral oncoprotein. Five tumours were classified as low-grade carcinoma on the basis of morphological criteria and calculation of mean nuclear area (MNA) and mean nuclear perimeter (MNP). Four tumours were classified as high-grade carcinoma. Sigma-2 receptors were overexpressed in both types of carcinoma. In control normal bovine bladder tissue the density of receptors (expressed as the B(max)) was 0.37 pmol/mg of protein. Low-grade carcinomas had a mean B(max) of 1.37+/-0.32 pmol/mg of protein (range 1.03-1.86) and in high-grade carcinomas the mean B(max) was 10.9+/-2.8 pmol/mg of protein (range 8.2-14). The difference in B(max) between low- and high-grade carcinomas was statistically significant (P=0.0001).
Roperto
S
S
Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Naples University Federico II, 80137 Naples, Italy. sante.roperto@unina.it
Colabufo
N A
NA
Inglese
C
C
Urraro
C
C
Brun
R
R
Mezza
E
E
Staibano
S
S
Raso
C
C
Maiolino
P
P
Russo
V
V
Palma
E
E
Roperto
F
F
D000818:Animals; D002277:Carcinoma / Q000378:metabolism* / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D047468:Immunoprecipitation; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism / Q000628:therapy* / Q000662:veterinary; D016133:Polymerase Chain Reaction; D017480:Receptors, sigma / Q000096:biosynthesis*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D017480:Receptors, sigma; C054304:oncogene protein E5, Bovine papillomavirus type 1; C106107:sigma-2 receptor