The possible role of myosin light chain in myoblast proliferation.
Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this report, we studied the possible role of Myl in the proliferation of skeletal muscle-derived myoblasts. Compared to diaphragm-derived myoblasts, the extraocular muscle-derived myoblasts with lower levels of Myl proliferated faster, maintained a longer proliferation phase, and formed more final myotubes. It was found that blockading Myl with anti-Myl antibody or knockdown of Myll by siRNA targeted against Myll could enhance the myoblast proliferation and delay the differentiation of myoblasts. Our results suggested that Myl, likely Myll, can negatively affect myoblast proliferation by facilitating myoblast withdrawal from cell cycle and differentiation.
Zhang
Su-Zhen
SZ
West China Hospital, Sichuan University, Chengdu, China.
Xu
Yong
Y
Xie
Hui-Qi
HQ
Li
Xiu-Qun
XQ
Wei
Yu-Quan
YQ
Yang
Zhi-Ming
ZM
D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D003964:Diaphragm / Q000166:cytology*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D032446:Myoblasts / Q000502:physiology*; D018994:Myosin Light Chains / Q000502:physiology*; D009801:Oculomotor Muscles / Q000166:cytology*; D012038:Regeneration / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018994:Myosin Light Chains