title: Steroid hormone receptors ERalpha and PR characterised by immunohistochemistry in the mare adrenal gland.
abstract: BACKGROUND
  Sex steroid hormone receptors have been identified in the adrenal gland of rat, sheep and rhesus monkey, indicating a direct effect of sex steroids on adrenal gland function.
  
  
  METHODS
  In the present study, immunohistochemistry using two different mouse monoclonal antibodies was employed to determine the presence of oestrogen receptor alpha (ERalpha) and progesterone receptor (PR) in the mare adrenal gland. Adrenal glands from intact (n = 5) and ovariectomised (OVX) (n = 5) mares, as well as uterine tissue (n = 9), were collected after euthanasia. Three of the OVX mares were treated with a single intramuscular injection of oestradiol benzoate (2.5 mg) 18-22 hours prior to euthanasia and tissue collection (OVX+Oe). Uterine tissue was used as a positive control and showed positive staining for both ERalpha and PR.
  
  
  RESULTS
  ERalpha staining was detected in the adrenal zona glomerulosa, fasciculata and reticularis of all mare groups. Ovariectomy increased cortical ERalpha staining intensity. In OVX mares and one intact mare, positive ERalpha staining was also detected in adrenal medullary cells. PR staining of weak intensity was present in a low proportion of cells in the zona fasciculata and reticularis of all mare groups. Weak PR staining was also found in a high proportion of adrenal medullary cells. In contrast to staining in the adrenal cortex, which was always located within the cell nuclei, medullary staining for both ERalpha and PR was observed only in the cell cytoplasm.
  
  
  CONCLUSIONS
  The present results show the presence of ERalpha in the adrenal cortex, indicating oestradiol may have a direct effect on mare adrenal function. However, further studies are needed to confirm the presence of PR as staining in the present study was only weak and/or minor. Also, any possible effect of oestradiol treatment on the levels of steroid receptors cannot be determined by the present study, as treatment time was of a too short duration.
authors.affiliation: University Animal Hospital, Swedish University of Agricultural Sciences, PO Box 7040, SE-75007 Uppsala, Sweden. ylva.hedberg.alm@uds.slu.se
authors.forename: Ylva Hedberg
authors.initials: YH
authors.lastname: Alm
authors.forename: Sayamon
authors.initials: S
authors.lastname: Sukjumlong
authors.forename: Hans
authors.initials: H
authors.lastname: Kindahl
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Dalin
mesh_terms: D000311:Adrenal Glands / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D047628:Estrogen Receptor alpha / Q000032:analysis*; D005260:Female; D006736:Horses / Q000378:metabolism*; D007150:Immunohistochemistry; D010052:Ovariectomy / Q000662:veterinary; D011897:Random Allocation; D011980:Receptors, Progesterone / Q000032:analysis*; D014599:Uterus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D011980:Receptors, Progesterone