Detection of herpes simplex virus dependent apoptosis.
Subversion of the host response to virus infection is a universal theme of virology and viral immunology. Multiple mechanisms are in place to limit virus spread on behalf of the host, yet through evolution, viruses have adapted to either weaken or eliminate the effects of these host factors. Cell death or apoptosis is one such example of a host response to viral infection. As such, experimental techniques that enable analysis of viruses (and viral genes) involved in triggering, blocking, or perhaps augmenting this process represent important tools for virologists, immunologists, and cell biologists. Presented here are a series of techniques developed in our lab for the analysis of apoptosis that occurs as a consequence of herpes simplex virus type 1 infection.
Cotter
Christopher R
CR
Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA.
Blaho
John A
JA
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000032:analysis / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology*; D045304:Cytochromes c / Q000032:analysis / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000821:virology*
D045304:Cytochromes c; D020169:Caspases