title: The GLABRA2 homeodomain protein directly regulates CESA5 and XTH17 gene expression in Arabidopsis roots.
abstract: Arabidopsis root hair formation is determined by the patterning genes CAPRICE (CPC), GLABRA3 (GL3), WEREWOLF (WER) and GLABRA2 (GL2), but little is known about the later changes in cell wall material during root hair formation. A combined Fourier-transform infrared microspectroscopy-principal components analysis (FTIR-PCA) method was used to detect subtle differences in the cell wall material between wild-type and root hair mutants in Arabidopsis. Among several root hair mutants, only the gl2 mutation affected root cell wall polysaccharides. Five of the 10 genes encoding cellulose synthase (CESA1-10) and 4 of 33 xyloglucan endotransglucosylase (XTH1-33) genes in Arabidopsis are expressed in the root, but only CESA5 and XTH17 were affected by the gl2 mutation. The L1-box sequence located in the promoter region of these genes was recognized by the GL2 protein. These results indicate that GL2 directly regulates cell wall-related gene expression during root development.
authors.affiliation: Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. twada@nibb.ac.jp
authors.forename: Rumi
authors.initials: R
authors.lastname: Tominaga-Wada
authors.forename: Mineko
authors.initials: M
authors.lastname: Iwata
authors.forename: Junji
authors.initials: J
authors.lastname: Sugiyama
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kotake
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ishida
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Yokoyama
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nishitani
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Okada
authors.forename: Takuji
authors.initials: T
authors.lastname: Wada
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D002482:Cellulose / Q000032:analysis / Q000096:biosynthesis; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D029681:Arabidopsis Proteins; C089991:GL2 protein, Arabidopsis; D018398:Homeodomain Proteins; D002482:Cellulose