Fatty acid profiling and proteomic analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide.
Non-thermal sterilization and microbial inactivation processes are currently receiving much attention in food and pharmaceutical industries. In particular, since supercritical carbon dioxide (SC-CO2) treatment, which is conducted at relatively low temperatures, is considered to be a promising alternative method to replace thermal sterilization processes that cannot be safely used in foods and bioactive materials. Although SC-CO2 has been applied to many microorganisms, the inactivation of microbial cells by SC-CO2 has only been evaluated by using a conventional viable cell count such as a plating method, by which it is not possible to systematically elucidate the microbial cell inactivation process. Therefore, in this study the physiological status of SC-CO2 treated Salmonella enterica serotype Typhimurium was analyzed by using GC-MS analysis of fatty acids with principal component analysis and two-dimensional electrophoresis for protein profiling. From the results of these systemic analyses, it was revealed that SC-CO2 caused significant alterations to the profiles of fatty acids and proteins of the cells.
Kim
Soo Rin
SR
School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
Kim
Hee Taek
HT
Park
Hee Jung
HJ
Kim
Sooah
S
Choi
Hee Jung
HJ
Hwang
Geum-Sook
GS
Yi
Jong Hoon
JH
Ryu
Do Hyun
DH
Kim
Kyoung Heon
KH
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D005516:Food Microbiology; D008401:Gas Chromatography-Mass Spectrometry; D050296:Microbial Viability / Q000187:drug effects; D025341:Principal Component Analysis; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000378:metabolism*; D013242:Sterilization / Q000379:methods
D001426:Bacterial Proteins; D005227:Fatty Acids; D020543:Proteome; D002245:Carbon Dioxide