[Comparison on HPLC fingerprint profiles of Lonicera japonica bud obtained by various dryness techniques].
OBJECTIVE
To investigate ingredient differences of Lonicera japonica bud processed by different dryness techniques.


METHODS
The HPLC fingerprint was used to evaluate the quality of L. japonica by various dryness process. The analysis was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm). Acetonitril and water containing 0.4% H3PO4 were used as mobile phases with gradient elution. The flow rate was set at 1.0 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was at 238 nm.


RESULTS
The HPLC-fingerprint profiles of L. japonica from six different dryness techniques were determined. A total of 17 common peaks were found, but their contents from various dryness techniques were significantly different.


CONCLUSIONS
HPLC-fingerprint technique was one of potential Process Analytical Techniques (PAT) to explore the ingredient differences in dryness process.
Xiong
Yan
Y
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Gao
Huimin
H
Wang
Zhiming
Z
Zhu
Jingjing
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031239:Lonicera / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results