title: [Application of serum protein markers to distinguish familial adenomatous polyposis (FAP) and sporadic colorectal adenomas].
abstract: OBJECTIVE
  To screen out specifically-expressed serum protein markers in familial adenomatous polyposis (FAP) and to establish a serum protein fingerprint diagnostic model for distinguishing FAP from sporadic colorectal adenomas.
  
  
  METHODS
  Serum samples were collected from 19 FAP cases and 16 sporadic colorectal adenomas with informed consent. Serum protein fingerprint profiles were detected by SELDI-TOF-MS with CM 10 protein chip to screen out FAP adenoma-related serum protein markers, and support vector machine (SVG) technique was used to establish the diagnostic model to distinguish FAP from sporadic colorectal adenomas.
  
  
  RESULTS
  Six differently-expressed protein peaks (P < 0.01) were detected. Among them proteins of 5640, 3160, 4180 and 4290 m/z were highly expressed in FAP adenomas, and proteins of 3940 and 3400 m/z were highly expressed in sporadic colorectal adenomas. The accuracy of diagnostic model established with SVG to distinguish FAP adenomas and sporadic colorectal adenomas was 94.7% and 93.7%, respectively.
  
  
  CONCLUSIONS
  SELDI-TOF-MS can be effectively used to screen out the differentially expressed serum protein markers in FAP adenomas and sporadic colorectal adenomas, and a diagnostic model build by SVG to distinguish them has been successfully established. Therefore, a useful breakthrough point for research on molecular mechanisms of FAP pathogenesis is provided.
authors.affiliation: The 2nd Affiliated Hospital, Medical School, Zhejiang University, Hangzhou 310009, China.
authors.forename: Shan-rong
authors.initials: SR
authors.lastname: Cai
authors.forename: Jie-kai
authors.initials: JK
authors.lastname: Yu
authors.forename: Wen-zhi
authors.initials: WZ
authors.lastname: Jiang
authors.forename: Su-zhan
authors.initials: SZ
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D011125:Adenomatous Polyposis Coli / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D003937:Diagnosis, Differential; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor