Binary adsorption of globular proteins on ion-exchange media.
Adsorption equilibrium of binary pairs of lysozyme (LYS), cytochrome c (CYC) and ribonuclease A (RNase) has been measured on different cation-exchange media at various solution conditions. Adsorption patterns largely follow the intrinsic protein-surface interactions, but can differ significantly for different pairs or even for one pair at different solution conditions. LYS/CYC adsorption shows similar behavior on all the adsorbents examined, with competitive adsorption dominated by LYS and the presence of LYS reducing the adsorption of CYC significantly. Simultaneous and sequential measurements for LYS/CYC show that the order of adsorption does not have a significant effect on the adsorption equilibrium. For LYS/RNase, LYS is consistently more strongly adsorbed. For CYC/RNase, both proteins can display significant adsorption, depending on the pH and salt concentration. A model based on colloidal energetics is developed to calculate the binary adsorption isotherms using parameter values obtained from single-component isotherms. The calculated adsorption is in good agreement with experimental results, with significantly better representation than for other commonly used binary isotherms.
Xu
Xuankuo
X
Center for Molecular and Engineering Thermodynamics, Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA.
Lenhoff
Abraham M
AM
D000327:Adsorption; D045304:Cytochromes c / Q000032:analysis / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D008958:Models, Molecular; D009113:Muramidase / Q000032:analysis / Q000302:isolation & purification; D009994:Osmolar Concentration; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012259:Ribonuclease, Pancreatic / Q000032:analysis / Q000302:isolation & purification
D007475:Ion Exchange Resins; D011506:Proteins; D045304:Cytochromes c; D012259:Ribonuclease, Pancreatic; C421864:hen egg lysozyme; D009113:Muramidase