Mapping of functional domains in herpesvirus saimiri complement control protein homolog: complement control protein domain 2 is the smallest structural unit displaying cofactor and decay-accelerating activities.
Herpesvirus saimiri encodes a functional homolog of human regulator-of-complement-activation proteins named CCPH that inactivates complement by accelerating the decay of C3 convertases and by serving as a cofactor in factor I-mediated inactivation of their subunits C3b and C4b. Here, we map the functional domains of CCPH. We demonstrate that short consensus repeat 2 (SCR2) is the minimum domain essential for classical/lectin pathway C3 convertase decay-accelerating activity as well as for factor I cofactor activity for C3b and C4b. Thus, CCPH is the first example wherein a single SCR domain has been shown to display complement regulatory functions.
Singh
Akhilesh K
AK
National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune 411007, India.
Yadav
Viveka Nand
VN
Pyaram
Kalyani
K
Mullick
Jayati
J
Sahu
Arvind
A
D019921:Biotinylation; D003179:Complement C3b / Q000737:chemistry; D015935:Complement C4b / Q000737:chemistry; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D017353:Gene Deletion; D006567:Herpesvirus 2, Saimiriine / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000737:chemistry; D008024:Ligands; D009154:Mutation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
D037102:Lectins; D008024:Ligands; D011994:Recombinant Proteins; D003179:Complement C3b; D015935:Complement C4b; D003165:Complement System Proteins