title: Albumin microspheres for oral delivery of iron.
abstract: Bovine serum albumin (BSA) microspheres of ferric pyrophosphate (FPP) intended for passive targeting to the Peyer's patches has been proposed for oral iron supplementation. Microspheres prepared by emulsification chemical cross linking method were characterized for surface topography, entrapment efficiency, particle size, particle charge and in vitro drug release. Microspheres of batch C with FPP to BSA ratio of 1:5 were found to be most suitable for targeting as they exhibited high entrapment (83.88 +/- 4.31), high monodispersity (span = 1.24 +/- 0.01), and least particle size (d(vm) = 4.40 +/- 0.01). In addition the amount of iron retained in these microspheres despite exposure to simulated gastrointestinal conditions for 5 h was found to be 83.72 +/- 4.22%, the highest in the three batches. The in vivo serum iron profiles in normal rats following oral administration displayed a reduced T(max) (2 h), elevated C(max) (106.06 +/- 12.18 mug/dL) and increased AUC (0-16 h) (647.44 +/- 52.33 mug.h/dL) for these microspheres which significantly differed (P <0.05) from FPP solution indicating a higher iron repletion potential of the BSA microspheres.
authors.affiliation: Department of Pharmaceutics, The University of Mississippi, University, Mississippi, USA.
authors.forename: H N
authors.initials: HN
authors.lastname: Shivakumar
authors.forename: Siva Ram Kiran
authors.initials: SR
authors.lastname: Vaka
authors.forename: S Narasimha
authors.initials: SN
authors.lastname: Murthy
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D011756:Diphosphates / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D007501:Iron / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008297:Male; D008863:Microspheres; D010316:Particle Size; D010581:Peyer's Patches / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013997:Time Factors
chemical_list: D011756:Diphosphates; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D007501:Iron; C049051:ferric pyrophosphate