title: Conformational heterogeneity of the M2 proton channel and a structural model for channel activation.
abstract: The M2 protein of influenza virus A is a proton-selective ion channel activated by pH. Structure determination by solid-state and solution NMR and X-ray crystallography has contributed significantly to our understanding, but channel activation may involve conformations not captured by these studies. Indeed, solid-state NMR data demonstrate that the M2 protein possesses significant conformational heterogeneity. Here, we report molecular dynamics (MD) simulations of the M2 transmembrane domain (TMD) in the absence and presence of the antiviral drug amantadine. The ensembles of MD conformations for both apo and bound forms reproduced the NMR data well. The TMD helix was found to kink around Gly-34, where water molecules penetrated deeply into the backbone. The amantadine-bound form exhibited a single peak approximately 10 degrees in the distribution of helix-kink angle, but the apo form exhibited 2 peaks, approximately 0 degrees and 40 degrees . Conformations of the apo form with small and large kink angles had narrow and wide pores, respectively, around the primary gate formed by His-37 and Trp-41. We propose a structural model for channel activation, in which the small-kink conformations dominate before proton uptake by His-37 from the exterior, and proton uptake makes the large-kink conformations more favorable, thereby priming His-37 for proton release to the interior.
authors.affiliation: Department of Physics, Florida State University, Tallahassee, FL 32306, USA.
authors.forename: Myunggi
authors.initials: M
authors.lastname: Yi
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Cross
authors.forename: Huan-Xiang
authors.initials: HX
authors.lastname: Zhou
mesh_terms: D000547:Amantadine / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry; D001665:Binding Sites; D003198:Computer Simulation; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000737:chemistry; D000086082:Viroporin Proteins
chemical_list: D000547:Amantadine; D000596:Amino Acids; D012492:Salts; D014763:Viral Matrix Proteins; D014867:Water; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins