[Tanshinone IIA down-regulates the expression of MMP-12 and TF in RAW 264.7 cells].
OBJECTIVE
To investigate the effect of tanshinone IIA (TanIIA) on the expression of tissue factor (TF) and matrix metalloproteinase-12 (MMP-12) in RAW264.7 cells and explore the possible mechanism.


METHODS
RAW 264.7 cells were incubated with ox-LDL in the presence or absence of different concentrations of tanshinone IIA. At the end of the incubation, the cell proliferation was assessed by MTT assay, and superoxide dismutase (SOD) activity and malondialdehyde (MDA) and TF concentrations in the supernatant were detected by xanthine oxidase method, thiobarbituric acid method and ELISA, respectively. Western blotting was employed to determine MMP-12 expression in the cells.


RESULTS
The cell proliferation was dose-dependently inhibited by TanIIA. SOD activity in the supernatant was increased significantly, while the MDA and TF concentration and MMP-12 expression in cells decreased after treatment of the cells with different concentrations of TanIIA.


CONCLUSIONS
TanIIA inhibits the cell proliferation and TF and MMP-12 expressions in RAW264.7 cells stimulated by ox-LDL, and these effects may be related with the anti-oxidation property of TanIIA.
Wang
Yan
Y
Second Department of Cardiovascular Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 514001, China. wy6806003@ 163.com
Fang
Zhi-Yuan
ZY
Huang
Shi-An
SA
Cui
Liao
L
D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D008077:Lipoproteins, LDL / Q000009:adverse effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D053508:Matrix Metalloproteinase 12 / Q000378:metabolism*; D051379:Mice; D013925:Thromboplastin / Q000378:metabolism*
D045784:Abietanes; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C021751:tanshinone; D008315:Malondialdehyde; D013925:Thromboplastin; D053508:Matrix Metalloproteinase 12