Normal pregnancy after tetraploid karyotype on trophectoderm biopsy.
OBJECTIVE
To report a case of successful pregnancy after trophectoderm biopsy and fluorescence in situ hybridization (FISH) revealed a tetraploid karyotype.


METHODS
Case report.


METHODS
A university medical center.


METHODS
An infertility patient desiring trophectoderm biopsy on frozen blastocysts to facilitate preimplantation genetic screening.


METHODS
Frozen blastocysts were thawed on the evening before transfer. Trophectoderm biopsy was performed the following morning. FISH results were available the same day, and two embryos with tetraploid results were transferred.


METHODS
Chorionic villus sample (CVS) and newborn exam.


RESULTS
Normal diploid CVS result and a healthy male infant.


CONCLUSIONS
Although multiple cells can be analyzed using trophectoderm biopsy, abnormalities in the trophectoderm may not be present in the inner cell mass.
Krieg
Sacha A
SA
Division of Reproductive Endocrinology and Infertility, Stanford Hospital and Clinics, Palo Alto, California.
Lathi
Ruth B
RB
Division of Reproductive Endocrinology and Infertility, Stanford Hospital and Clinics, Palo Alto, California.
Behr
Barry
B
Division of Reproductive Endocrinology and Infertility, Stanford Hospital and Clinics, Palo Alto, California.
Westphal
Lynn M
LM
Division of Reproductive Endocrinology and Infertility, Stanford Hospital and Clinics, Palo Alto, California. Electronic address: lynnw@stanford.edu.
D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000473:pathology*; D002869:Chromosome Aberrations; D004475:Ectoderm / Q000473:pathology*; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D007247:Infertility, Female / Q000628:therapy; D008297:Male; D011003:Ploidies; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000235:genetics*; D019836:Preimplantation Diagnosis / Q000379:methods*