title: Effects of surface carboxylic acid groups of cerasomes, morphologically stable vesicles having a silica surface, on biomimetic deposition of hydroxyapatite in body fluid conditions.
abstract: Biomimetic mineralization of supramolecular scaffolds consisting of biomolecules or their analogues has received much attention recently from the viewpoint of creation of novel biomaterials. This study investigated biomimetic deposition of hydroxyapatite (HAp) on cerasomes, morphologically stable organic-inorganic hybrid vesicles. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction studies revealed that the pristine cerasomes induced heterogeneous nucleation of HAp when they were immersed in 1.5SBF, a solution having 1.5 times higher ion concentration than that of a simulated body fluid (SBF). The HAp deposition was further accelerated when dicarboxylic and monocarboxylic acid groups were displayed on cerasome surfaces. These carboxylic acid groups are expected to enhance calcium ion binding to the cerasome surface, causing an increase of HAp nucleation sites. At lower surface concentrations on the cerasome surface, the dicarboxylic acid group is apparently more effective for HAp deposition than the monocarboxylic acid group. The resultant HAp-cerasome hybrids are useful as biocompatible materials having unique properties deriving from the lipid bilayer structure of the cerasomes.
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), Ikoma, Nara, Japan.
authors.forename: Mineo
authors.initials: M
authors.lastname: Hashizume
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horii
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kikuchi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kamitakahara
authors.forename: Chikara
authors.initials: C
authors.lastname: Ohtsuki
authors.forename: Masao
authors.initials: M
authors.lastname: Tanihara
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002264:Carboxylic Acids / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D004355:Drug Stability; D017886:Durapatite / Q000493:pharmacokinetics*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D008422:Materials Testing; D008954:Models, Biological; D012822:Silicon Dioxide / Q000737:chemistry* / Q000494:pharmacology; D012833:Siloxanes / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; D020099:Coated Materials, Biocompatible; D008081:Liposomes; D012833:Siloxanes; D012822:Silicon Dioxide; D017886:Durapatite