Effects of hydrostatic pressure on the stability and thermostability of poliovirus: a new method for vaccine preservation.
Viruses are a structurally diverse group of infectious agents that differ widely in their sensitivities to high hydrostatic pressure (HHP). Studies on picornaviruses have demonstrated that these viruses are extremely resistant to HHP treatments, with poliovirus appearing to be the most resistant. Here, the three attenuated poliovirus serotypes were compared with regard to pressure and thermal resistance. We found that HHP does not inactivate any of the three serotypes studied (1-3). Rather, HHP treatment was found to stabilize poliovirus by increasing viral thermal resistance at 37 degrees C. Identification of new methods that stabilize poliovirus against heat inactivation would aid in the design of a more heat-stable vaccine, circumventing the problems associated with refrigeration during storage and transport of the vaccine prior to use.
Ferreira
Evanilce
E
Programa de Vacinas Virais, Instituto de Tecnologia em Imunobiológicos, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, Brazil.
Mendes
Ygara S
YS
Silva
Jerson L
JL
Galler
Ricardo
R
Oliveira
Andréa C
AC
Freire
Marcos S
MS
Gaspar
Luciane P
LP
D000818:Animals; D002522:Chlorocebus aethiops; D006358:Hot Temperature; D006801:Humans; D006874:Hydrostatic Pressure; D017955:Poliovirus / Q000502:physiology*; D023321:Poliovirus Vaccines / Q000494:pharmacology; D011309:Preservation, Biological; D014709:Vero Cells; D014779:Virus Replication
D023321:Poliovirus Vaccines