title: Activation of translation via reduction by thioredoxin-thioredoxin reductase in Saccharomyces cerevisiae.
abstract: Previously we reported that in vitro translation activity in extracts of Saccharomyces cerevisiae was stimulated by dithiothreitol (DTT) and further increased by the addition of thioredoxin (TRX1) [Choi, S.K. (2007) Thioredoxin-mediated regulation of protein synthesis by redox in Saccharomyces cerevisiae. Kor. J. Microbiol. Biotechnol. 35, 36-40]. To identify the pathway affecting translation, we cloned and purified thioredoxin reductase 1 (TRR1), thioredoxin reductase 2 (TRR2), glutaredoxin 1 (GRX1) and glutaredoxin reductase 1 (GLR1) as fusion proteins. Thioredoxin-mediated activation of translation was more effectively stimulated by NADPH or NADH than by DTT. Moreover, addition of TRR1 led to a further increase of translation in the presence of thioredoxin plus NADPH. These findings indicate that redox control via the thioredoxin-thioredoxin reductase system plays an important role in the regulation of translation.
authors.affiliation: Department of Biological Sciences, College of Life Industry and Science, Sunchon National University, Jeonnam, Republic of Korea.
authors.forename: Kyung Ok
authors.initials: KO
authors.lastname: Jun
authors.forename: Chin-Hee
authors.initials: CH
authors.lastname: Song
authors.forename: Young-Bum
authors.initials: YB
authors.lastname: Kim
authors.forename: Jinseok
authors.initials: J
authors.lastname: An
authors.forename: Jae Hyeun
authors.initials: JH
authors.lastname: Oh
authors.forename: Sang Ki
authors.initials: SK
authors.lastname: Choi
mesh_terms: D015966:Gene Expression Regulation, Fungal; D054477:Glutaredoxins / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D054481:Thioredoxin Reductase 1 / Q000235:genetics / Q000378:metabolism*; D054482:Thioredoxin Reductase 2 / Q000235:genetics / Q000378:metabolism*; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D054477:Glutaredoxins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D009243:NAD; D013879:Thioredoxins; D009249:NADP; D054481:Thioredoxin Reductase 1; D054482:Thioredoxin Reductase 2