title: Adrenomedullin, Bcl-2 and microvessel density in normal, hyperplastic and neoplastic endometrium.
abstract: Adrenomedullin (ADM) is a multifunctional 52-amino acid peptide involved in numerous physiological and pathological processes, including angiogenesis, growth regulation, differentiation, and vasodilation. ADM is thought to act through the G protein-coupled receptor calcitonin receptor-like receptor, with specificity being conferred by receptor-associated modifying protein 2. The aim of the present study was to clarify the roles of ADM status, and tumor vessels in endometrium. Specimens were examined for ADM, microvessel density (MVD), area of venules (AV) and Bcl-2 oncoprotein using an immunoperoxidase method. The difference of ADM between normal proliferative phase and hyperplasia without atypia was significant (P < 0.05). The level of Bcl-2 was significantly different between hyperplasia without atypia and hyperplasia with atypia (P < 0.05). ADM, MVD and AV in the endometrium increased in a stepwise manner from normal, simple or complex hyperplasia with or without atypia to grade 1 adenocarcinoma. In contrast, expression of Bcl-2 oncoprotein was decreased. These parameters identify the role of ADM expression and Bcl-2 protein in relation to cell growth and vasodilating in the neoplastic changes.
authors.affiliation: Department of Medical Technology, Kobe Tokiwa University, Kobe, Hyogo, Japan. nunobiki@kobe-tokiwa.ac.jp
authors.forename: Osamu
authors.initials: O
authors.lastname: Nunobiki
authors.forename: Misa
authors.initials: M
authors.lastname: Nakamura
authors.forename: Emiko
authors.initials: E
authors.lastname: Taniguchi
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Utsunomiya
authors.forename: Ichiro
authors.initials: I
authors.lastname: Mori
authors.forename: Yukari
authors.initials: Y
authors.lastname: Tsubota
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Mabuchi
authors.forename: Kennichi
authors.initials: K
authors.lastname: Kakudo
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D053607:Adrenomedullin / Q000096:biosynthesis*; D000328:Adult; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000098:blood supply / Q000473:pathology; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D055806:Microvessels; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D053607:Adrenomedullin