title: Simultaneous determination of oxyresveratrol and resveratrol in rat bile and urine by HPLC after oral administration of Smilax china extract.
abstract: Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4'-trihydroxystilbene, RES) are the two most important constituents of the traditional Chinese medicine Smilax china. A reversed-phase high-performance liquid chromatographic method was developed to determine OXY and RES in rat bile and urine after oral administration of Smilax china extract. The biological samples were analyzed by HPLC on Aglient Zorbax SB-C18 column (250 x 4.6 mm, 5 microm) at a wavelength 320 nm and at a flow rate of 1.0 mL/min. The method was accurate and reproducible for determination. The cumulative excretion of OXY and RES was 0.29% and 0.97% in bile samples, 0.84% and 0.65% in urine samples, respectively.
authors.affiliation: Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, PR China.
authors.forename: Hui-lian
authors.initials: HL
authors.lastname: Huang
authors.forename: Jin-qiang
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Guang-tong
authors.initials: GT
authors.lastname: Chena
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Lu
authors.forename: Na
authors.initials: N
authors.lastname: Sha
authors.forename: De-an
authors.initials: DA
authors.lastname: Guo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D012680:Sensitivity and Specificity; D032281:Smilax / Q000737:chemistry*; D013267:Stilbenes / Q000737:chemistry* / Q000378:metabolism / Q000652:urine
chemical_list: D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D000077185:Resveratrol