title: Rapid determination of phenolic compounds and alkaloids of carob flour by improved liquid chromatography tandem mass spectrometry.
abstract: An improved chromatographic method was developed using ultra-performance liquid chromatography-tandem mass spectrometry to identify and quantify phenolic compounds and alkaloids, theobromine and caffeine, in carob flour samples. The developed method has been validated in terms of speed, sensitivity, selectivity, peak efficiency, linearity, reproducibility, limits of detection, and limits of quantification. The chromatographic method allows the identification and quantification of 20 phenolic compounds, that is, phenolic acids, flavonoids, and their aglycone and glucoside forms, together with the determination of the alkaloids, caffeine and theobromine, at low concentration levels all in a short analysis time of less than 20 min.
authors.affiliation: Food Technology Department, XaRTA-UTPV, Escuela Tecnica Superior de Ingenieria Agraria Universidad de Lleida, Av/Alcalde Rovira Roure 191, Lleida, Spain.
authors.forename: Nàdia
authors.initials: N
authors.lastname: Ortega
authors.forename: Alba
authors.initials: A
authors.lastname: Macià
authors.forename: Maria-Paz
authors.initials: MP
authors.lastname: Romero
authors.forename: Esther
authors.initials: E
authors.lastname: Trullols
authors.forename: Jose-Ramón
authors.initials: JR
authors.lastname: Morello
authors.forename: Neus
authors.initials: N
authors.lastname: Anglès
authors.forename: Maria-Jose
authors.initials: MJ
authors.lastname: Motilva
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005433:Flour / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D010636:Phenols