Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor.
We report Hepatitis B Virus (HBV) DNA detection using a silica nanoparticle-enhanced dynamic microcantilever biosensor. A 243-mer nucleotide of HBV DNA precore/core region was used as the target DNA. For this assay, the capture probe on the microcantilever surface and the detection probe conjugated with silica nanoparticles were designed specifically for the target DNA. For efficient detection of the HBV target DNA using silica nanoparticle-enhanced DNA assay, the size of silica nanoparticles and the dimension of microcantilever were optimized by directly binding the silica nanoparticles through DNA hybridization. In addition, the correlation between the applied nanoparticle concentrations and the resonant frequency shifts of the microcantilever was discussed clearly to validate the quantitative relationship between mass loading and resonant frequency shift. HBV target DNAs of 23.1 fM to 2.31 nM which were obtained from the PCR product were detected using a silica nanoparticle-enhanced microcantilever. The HBV target DNA of 243-mer was detected up to the picomolar (pM) level without nanoparticle enhancement and up to the femtomolar (fM) level using a nanoparticle-based signal amplification process. In the above two cases, the resonant frequency shifts were found to be linearly correlated with the concentrations of HBV target DNAs. We believe that this linearity originated mainly from an increase in mass that resulted from binding between the probe DNA and HBV PCR product, and between HBV PCR product and silica nanoparticles for the signal enhancement, even though there is another potential factor such as the spring constant change that may have influenced on the resonant frequency of the microcantilever.
Cha
Byung Hak
BH
Nano Bio Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
Lee
Sang-Myung
SM
Park
Jae Chan
JC
Hwang
Kyo Seon
KS
Kim
Sang Kyung
SK
Lee
Yoon-Sik
YS
Ju
Byeong-Kwon
BK
Kim
Tae Song
TS
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis*; D004867:Equipment Design; D006515:Hepatitis B virus / Q000235:genetics*; D053758:Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D004269:DNA, Bacterial; D012822:Silicon Dioxide