The male sterility locus ms3 is present in a fertility controlling gene cluster in soybean.
Soybean [Glycine max (L.) Merr.] is self-pollinated. To produce large quantities of hybrid seed, insect-mediated cross-pollination is necessary. An efficient nuclear male-sterile system for hybrid seed production would benefit from molecular and/or phenotypic markers linked to male fertility/sterility loci to facilitate early identification of phenotypes. Nuclear male-sterile, female-fertile ms3 mutant is a single recessive gene and displays high outcrossed seed set with pollinators. Our objective was to map the ms3 locus. A segregating population of 150 F(2) plants from Minsoy (PI 27890) x T284H, Ms3ms3 (A00-68), was screened with 231 simple sequence repeat markers. The ms3 locus mapped to molecular linkage group (MLG) D1b (Gm02) and is flanked by markers Satt157 and Satt542, with a distance of 3.7 and 12.3 cM, respectively. Female-partial sterile-1 (Fsp1) and the Midwest Oilseed male-sterile (msMOS) mutants previously were located on MLG D1b. msMOS and Fsp1 are independent genes located very close to each other. All 3 genes are located in close proximity of Satt157. We believe that this is the first report of clustering of fertility-related genes in plants. Characterization of these closely linked genes may help in understanding the evolutionary relationship among them.
Cervantes-Martinez
Innan
I
Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA.
Sandhu
Devinder
D
Xu
Min
M
Ortiz-Pérez
Evelyn
E
Kato
Kiyoaki K
KK
Horner
Harry T
HT
Palmer
Reid G
RG
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D017343:Genes, Plant / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*