title: No association between EGF +61 A/G polymorphism and increased risk of glioma.
abstract: A single nucleotide polymorphism (SNP) of the epidermal growth factor (EGF) gene +61 A/G in the 5'-untranslated region has been reported to be associated with susceptibility to glioma. A case-control study (168 glioma patients and 194 normal controls) was conducted to elucidate its possible association with the risk of glioma in the Chinese population. Polymerase chain reaction-restriction fragment length polymorphism assay was used to analyze the EGF genotypes. The genotyping results were further confirmed by direct sequencing. The EGF +61A and +61G allele frequencies in the glioma group were 32.1% and 67.9%, respectively, while they were 30.4% and 69.6% in the healthy controls. Furthermore, the frequency of the A/A, A/G and G/G genotypes in glioma patients was 8.9%, 46.4%, and 44.7%, respectively, and 8.3%, 44.3%, and 47.4% in controls. There was no significant difference between patients and healthy controls. The EGF +61 A/G and +61 G/G genotypes were not significantly associated with risk of glioma compared with the A/A genotype. In addition, no significant association was observed between EGF polymorphism and different histological grades of glioma. These results indicate that the EGF +61 A/G polymorphism is not associated with susceptibility to glioma in the Chinese population. In addition, a literature review revealed a significantly higher rate of the A/A genotype in Caucasian compared with East Asian subjects. Such differences in genotype distribution between Caucasian and Asian people should be taken into account in future studies.
authors.affiliation: The Fourth Affiliated Hospital, Harbin Medical University, Heilongjiang Province, PR China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Chen
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Luyan
authors.initials: L
authors.lastname: Mu
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.forename: Fengmin
authors.initials: F
authors.lastname: Lu
mesh_terms: D020121:5' Untranslated Regions; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D004815:Epidermal Growth Factor / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005910:Glioma / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012306:Risk; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions; D004815:Epidermal Growth Factor