title: The Y chromosome of the Atelidae family (Platyrrhini): study by chromosome microdissection.
abstract: In order to study the intergeneric variability of the Y chromosome, we describe the hybridization of the Y chromosome of Brachytelesarachnoides, obtained by microdissection, to metaphases of Atelesbelzebuthmarginatus, Lagothrixlagothricha, and Alouatta male specimens. Brachytelesarachnoides (Atelinae) has 62 chromosomes and a very small Y chromosome. Our results showed that the Brachytelesarachnoides Y chromosome probe hybridized to Lagothrixlagothricha metaphases yielding one hybridization signal on only the tiny Y chromosome, and when hybridized with Atelesbelzebuthmarginatus metaphases it yielded one hybridization signal on two thirds of the small acrocentric Y chromosome. However, no hybridization signal was observed in Alouatta metaphases (subfamily Alouattinae), a closely related genus in the Atelidae family. Furthermore, our data support a close phylogenetic relationship among Brachyteles, Ateles, and Lagothrix and their placement in the Atelinae subfamily, but exclude Alouatta from this group indicating its placement as basal to this group.
authors.affiliation: Centro de Estudos do Genoma Humano, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil. crisgiughetti@yahoo.com.br
authors.forename: C
authors.initials: C
authors.lastname: Gifalli-Iughetti
authors.forename: C P
authors.initials: CP
authors.lastname: Koiffmann
mesh_terms: D000508:Alouatta / Q000145:classification / Q000235:genetics; D000818:Animals; D051077:Atelidae / Q000145:classification / Q000235:genetics*; D053819:Atelinae / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D042282:Microdissection / Q000379:methods; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D017931:DNA Primers