title: Evidence of okadaic acid production in a cultured strain of the marine dinoflagellate Prorocentrum rhathymum from Malaysia.
abstract: Protein phosphatase inhibition assay (PPIA), Neuroblastoma cell-based assay (Neuro-2a CBA) and LC-MS/MS analysis revealed for the first time the production of okadaic acid (OA) by a Prorocentrum rhathymum strain. Low amounts of OA were detected by LC-MS/MS analysis. Inhibition of PP2A activity and a weak toxicity to the Neuro-2a CBA were also observed.
authors.affiliation: IRTA, Ctra. Poble Nou, km. 5.5, 43540 Sant Carles de la Ràpita, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Caillaud
authors.forename: P
authors.initials: P
authors.lastname: de la Iglesia
authors.forename: M
authors.initials: M
authors.lastname: Campàs
authors.forename: L
authors.initials: L
authors.lastname: Elandaloussi
authors.forename: M
authors.initials: M
authors.lastname: Fernández
authors.forename: N
authors.initials: N
authors.lastname: Mohammad-Noor
authors.forename: K
authors.initials: K
authors.lastname: Andree
authors.forename: J
authors.initials: J
authors.lastname: Diogène
mesh_terms: D000818:Animals; D001681:Biological Assay; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004141:Dinoflagellida / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008296:Malaysia; D013058:Mass Spectrometry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D019319:Okadaic Acid / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019319:Okadaic Acid; D004247:DNA; D010749:Phosphoprotein Phosphatases