Tissue-specific requirements for FGF8 during early inner ear development.
Several members of the FGF gene family have been shown to intervene from various tissue sources to direct otic placode induction and otic vesicle formation. In this study we define the roles of FGF8, found in different expression domains during this process, in mice and chickens. By conditional inactivation of Fgf8 in distinct tissue compartments we demonstrate that Fgf8 is required in the mesoderm and endoderm during early inner ear development. In the chicken embryo, overexpression of Fgf8 from various tissue sources during otic specification leads to a loss of otic tissue. In contrast ectopic overexpression of Fgf10, a major player during murine otic induction, does not influence otic vesicle formation in chicken embryos but results in the formation of ectopic structures with a non-otic character. This study underlines the crucial role of a defined Fgf8 expression pattern controlling inner ear formation in vertebrates.
Domínguez-Frutos
Elena
E
Instituto de Biología y Genética Molecular, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), C/Sanz y Forés s/n, E-47003 Valladolid, Spain.
Vendrell
Victor
V
Alvarez
Yolanda
Y
Zelarayan
Laura Cecilia
LC
López-Hernández
Iris
I
Ros
Marian
M
Schimmang
Thomas
T
D000818:Animals; D002642:Chick Embryo; D007758:Ear, Inner / Q000196:embryology* / Q000502:physiology; D051518:Fibroblast Growth Factor 3 / Q000235:genetics / Q000502:physiology; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic
C489197:Fgf3 protein, mouse; C496457:Fgf8 protein, mouse; D051518:Fibroblast Growth Factor 3; D051524:Fibroblast Growth Factor 8