title: Peptide derived from the lipid binding domain of Group IB human pancreatic phospholipase A(2) possesses antibacterial activity.
abstract: Group 1B human pancreatic secretory phospholipase A(2) (hp-sPLA(2)), a digestive enzyme synthesized by pancreatic acinar cells and present in pancreatic juice, do not have antibacterial activity towards Escherichia coli. Our earlier results suggest that the N-terminal first ten amino acid residues of hp-sPLA(2) constitute major portion of the membrane binding domain of full-length enzyme and is responsible for the precise orientation of enzyme on the membrane surface by inserting into the lipid bilayers (Pande et al. (2006) Biochemistry, 45,12436-12447). In this study we report the antibacterial properties of a peptide (AVWQFRKMIK-CONH(2); N10 peptide), which corresponds to the N-terminal first ten amino acid residues of hp-sPLA(2), against E. coli. Full-length hp-sPLA(2), which contains this peptide sequence as N-terminal alpha-helix, did not showed detectable antibacterial activity. Presence of physiological concentration of salt or preincubation of N10 peptide with soluble anionic polymer inhibits the antibacterial activity indicating the importance of electrostatic interaction in binding of peptide to bacterial membrane. Addition of peptide resulted in destabilization of outer as well as inner cytoplasmic membrane of E. coli suggesting bacterial membranes to be the main target of action. N10 peptide exhibits strong synergism with lysozyme and potentiates the antibacterial activity of lysozyme. The peptide was inactive against human erythrocyte. Our result shows for the first time that a peptide fragment of hp-sPLA(2) possesses antibacterial activity towards E. coli and at subinhibitory concentration and can potentiate the antibacterial activity of membrane active enzyme. These observations suggest that N10 peptide may play an important role in the antimicrobial activity of pancreatic juice.
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar (Mohali), Punjab 160062, India.
authors.forename: Mehulkumar S
authors.initials: MS
authors.lastname: Limbachiya
authors.forename: Abhay H
authors.initials: AH
authors.lastname: Pande
mesh_terms: D000595:Amino Acid Sequence / Q000187:drug effects; D000818:Animals; D001665:Binding Sites; D002942:Circular Dichroism; D004357:Drug Synergism; D006801:Humans; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D010179:Pancreas / Q000201:enzymology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010741:Phospholipases A / Q000737:chemistry* / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects* / Q000502:physiology; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary / Q000187:drug effects
chemical_list: D010455:Peptides; D010741:Phospholipases A