Molecular characterization and functional commonality of nucleophosmin/nucleoplasmin in two cyprinid fish.
Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
Wu
Nan
N
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Graduate School of the Chinese Academy of Sciences, Wuhan, China.
Li
Chang-Jian
CJ
Gui
Jian-Fang
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006054:Goldfish / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D000090243:Nucleophosmin; D056489:Nucleoplasmins / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000166:cytology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000166:cytology; D014018:Tissue Distribution
D009687:Nuclear Proteins; D056489:Nucleoplasmins; D000090243:Nucleophosmin