Genotoxicity monitoring of freshwater environments using caged carp (Cyprinus carpio).
The present study deals with genotoxicity assessment of freshwaters using caged carp (Cyprinus carpio). Carps were transplanted from a fish-farm to three differently polluted sites in eastern Croatia. Two polluted sites were situated in the river Drava, downstream from the cities of Belisće and Osijek, while the reference site was in the Nature Park Kopacki rit, a preserved wetland area with limited anthropogenic influence. Exposure lasted for 3 weeks and was repeated for 3 years (2002-2004). DNA damage was assessed in erythrocytes of the exposed animals by the Comet assay and micronucleus test (MNT). In order to evaluate possible differences in stress responses to polluted water in situ and in aquaria a laboratory exposure was performed with water from the studied location in the second year of the study. Carp from the sites with high anthropogenic influence (Belisće and Osijek) had higher average DNA damage as expressed in both the MNT and Comet assay. Of the two, the Comet assay appeared to be more sensitive following both caging and aquaria exposures. The results from this study suggest that 3 weeks caging exposure of C. carpio may be a useful strategy to monitor for genotoxic agents in freshwater ecosystems.
Klobucar
Göran I V
GI
Department of Zoology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia. gklobuca@zg.biol.pmf.hr
Stambuk
Anamaria
A
Pavlica
Mirjana
M
Sertić Perić
Mirela
M
Kutuzović Hackenberger
Branimir
B
Hylland
Ketil
K
D000818:Animals; D002347:Carps / Q000378:metabolism*; D020552:Comet Assay; D017523:Croatia; D004249:DNA Damage / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods*; D015162:Micronucleus Tests; D045483:Rivers; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical