Molecular characterization and expression analysis of Acmago and AcY14 in Antrodia cinnamomea.
Mago nashi (Mago) and Y14 proteins, highly conserved among eukaryotes, participate in mRNA localization and splicing, and as such play important roles in oogenesis, embryogenesis and germ-line sex determination during animal development. Here we identified mago (Acmago) and Y14 (AcY14) homologues derived from Antrodia cinnamomea. Acmago encodes 149 amino acids and AcY14 encodes 168 amino acids. Multiple amino acid sequence alignment as well as secondary and tertiary structure prediction showed that AcMago and AcY14 have similar protein structure to the reported crystal structures of other Mago and Y14 proteins. During fungal development both Acmago and AcY14 genes were abundantly expressed in natural basidiomes. This is the first report of the molecular characterization and expression analysis of the mago and Y14 genes from fungi.
Chu
Fang-Hua
FH
School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan. fhchu@ntu.edu.tw <fhchu@ntu.edu.tw>
Chen
Yu-Rong
YR
Lee
Chen-Hsien
CH
Chang
Tun-Tschu
TT
D000595:Amino Acid Sequence; D055491:Antrodia / Q000235:genetics*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010802:Phylogeny; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005656:Fungal Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins