Entamoeba dispar: genetic diversity of Iranian isolates based on serine-rich Entamoeba dispar protein gene.
The nucleotide sequences of Serine-Rich Entamoeba histolytica Protein (SREHP) gene have already exhibited stable and significant polymorphism in the gene studies. Serine-rich protein is also present and polymorphic in Entamoeba dispar which called SREDP. The polymorphism of the Serine-Rich Entamoeba dispar Protein (SREDP) gene among 8 isolates obtained from Iranian cyst carriers were analyzed by a nested PCR-RFLP followed by sequencing of the PCR products. From those isolates, six distinct DNA patterns were observed after PCR-RFLP of the nested PCR, whereas sequencing showed 8 different patterns among the isolates. The results demonstrate an extensive genetic variability among Iranian E. dispar isolates. The repeat-containing region of the SREDP was found extensively polymorphic in size, number and order of repeat units. Genetic diversity of Iranian E. dispar isolates based on the SREDP was more polymorphic in comparison of Serine-Rich Entamoeba histolytica Protein (SREHP) of the E. histolytica isolates as well as were different from a few known SREDP genes.
Haghighi
A
A
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University, Medical Campus, Tehran, Iran.
Rasti
S
S
Nazemalhosseini Mojarad
E
E
Kazemi
B
B
Bandehpour
M
M
Nochi
Z
Z
Hooshyar
H
H
Rezaian
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D004747:Entamoeba / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D014644:Genetic Variation; D006801:Humans; D007492:Iran; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
D016054:DNA, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins; C073020:serine-rich protein, Entamoeba histolytica