title: [Survey on host animal and molecular epidemiology of hantavirus in Chuxiong prefecture, Yunnan province].
abstract: OBJECTIVE
  To determine the hosts of hantavirus (HV) and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome (HFRS).
  
  
  METHODS
  Rodents were captured by a special trap within the residential area. The antigens of HV in lung tissues were detected by direct immuno-fluorescence assay (DFA). Nucleotide sequences of HV were amplified by RT-PCR with HV genotype-specific primer. The amplified genes were then sequenced. Phylogenetic tree were built on nucleotide sequence with ClustalX 1.83 software.
  
  
  RESULTS
  1421 rodents were captured and classified into 8 species of 4 Genera in the epidemic area within 10 counties of Chuxiong prefecture, Yunnan province, between 2005 and 2006. Out of the 1421 rodents, 1056 (74.31% ) of them were Rattus norvegicus and 280 (19.70%) belonged to Rattus flavipectus. The antigens of HV were detected by DFA in lung tissues and the total positive rate of HV was 5.15% (53/ 1029). After applying the sequencing nucleotide method to the 53 positive specimens, data showed that 21 specimens were positive and all of them belonged to Seoul type (15 samples were from Rattus norvegicus, 4 samples Rattus flavipectus, 2 samples Rattus nitidus). The partial S segments from 12 specimens were sequenced which appeared homologic with R22, L99 and HLD65 from GenBank in relatively high level (87.1% -99.7%). When compared to 76-118 strain of Hantaan type, their homologic degree was only 64.4%-69.1%. Results from Phylogenetic analysis showed that 12 specimens belonged to Seoul type. As for their homology, they were significantly similar to Seoul type and could be tentatively divided into two subtypes S1 and S3.
  
  
  CONCLUSIONS
  It was confirmed that the Seoul type virus, as HFRS's pathogenetic agent mainly carried by rats, prevailed widely in Chuxiong prefecture. Owing to the local ecological environment, we also noticed the characteristics of different HV subtypes among Seoul type.
authors.affiliation: Yunnan Institute of Endemic Disease Control and Prevention, Dali 671000, China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Zhou
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Zhang
authors.forename: Jing-Lin
authors.initials: JL
authors.lastname: Wang
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Yang
authors.forename: Zhu-Qing
authors.initials: ZQ
authors.lastname: Mi
authors.forename: Yun-Zhi
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Xian-Yi
authors.initials: XY
authors.lastname: Song
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Hu
authors.forename: Ying-Kuan
authors.initials: YK
authors.lastname: Dong
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Pu
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Hu
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Gao
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Yuan
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Ya
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D002681:China / Q000453:epidemiology; D004199:Disease Vectors; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029263:Seoul virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017423:Sequence Analysis, RNA
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral