Investigation of extractive microbial transformation in nonionic surfactant micelle aqueous solution using response surface methodology.
Extractive microbial transformation of L-phenylacetylcarbinol (L-PAC) in nonionic surfactant Triton X-100 micelle aqueous solution was investigated by response surface methodology. Based on the Box-Behnken design, a mathematical model was developed for the predication of mutual interactions between benzaldehyde, Triton X-100, and glucose on L-PAC production. It indicated that the negative or positive effect of nonionic surfactant strongly depended on the substrate concentration. The model predicted that the optimal concentration of benzaldehyde, Triton X-100, and glucose was 1.2 ml, 15 g, and 2.76 g per 100 ml, respectively. Under the optimal condition, the maximum L-PAC production was 27.6 mM, which was verified by a time course of extractive microbial transformation. A discrete fed-batch process for verification of cell activity was also presented.
Xue
Yingying
Y
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Qian
Chen
C
Wang
Zhilong
Z
Xu
Jian-He
JH
Yang
Rude
R
Qi
Hanshi
H
D000096:Acetone / Q000031:analogs & derivatives* / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D005947:Glucose / Q000378:metabolism; D008823:Micelles; D008962:Models, Theoretical; D012996:Solutions; D013501:Surface-Active Agents / Q000494:pharmacology*
D001547:Benzaldehydes; D008823:Micelles; D012996:Solutions; D013501:Surface-Active Agents; D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; D005947:Glucose; C032175:benzaldehyde