[Determination of quercitrin in Polygonum capitatum and relinqing granules by HPLC].
OBJECTIVE
To establish an HPLC method for determination the contents of quercitrin in Polygonum capitatum and Relinqing granules.


METHODS
The samples were analyzed on a Diamonsil C18 colunm (4.6 mm x 250 mm, 5 microm) eluted with the mobile phase consisted of methanol-1% HAc-THF. The flow rate was 1.0 mL x min-m. The detection wavelength was set at 258 nm and the column temperature was 25 degrees C.


RESULTS
The calibration curve was linear in the range of 0. 081 64-0.4084 microg (r = 0.99997). The average recovery rate of quercitrin in P. capitatum was 102.3% (RSD 0.99%), and was 102.7% (RSD 2.2%) in Relinqing granules.


CONCLUSIONS
The method is reliable and specific with good repeatability, and can be used for the quality control of P. capitatum and Relinqing granules. It can provide a science bases for the planting of polygonum capitatum.
Xie
Yu
Y
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
Zhang
Liyan
L
Liang
Bin
B
Li
Menglin
M
Tang
Jingwen
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; C012526:quercitrin; D011794:Quercetin