title: [Construction of attenuated Salmonella choleraesuis vaccine strain expressing recombinant antigen of Shiga-like toxin Escherichia coli].
abstract: OBJECTIVE
  To construct an attenuated Salmonella choleraesuis vaccine strain expressing the gene SLT-IIeB and FedF of Shiga-like toxin Escherichia coli (SLTEC) O138 with balanced lethal system.
  
  
  METHODS
  The gene of SLT-IIeB and FedF of SLTEC O138 was amplified, and then recombined with a vector pYA3493 (Asd+). The recombinant was electroporated into attenuated Salmonella choleraesuis C500 (Asd-). The expression of SLT-IIeB and FedF was analyzed by SDS-PAGE. The stability of the vaccine strains was studied by generation culture in vitro.
  
  
  RESULTS
  The stable attenuated Salmonella choleraesuis vaccine strain expressing SLT-IIeB and FedF of SLTEC O138 was constructed with balanced lethal system. The expressed products with protein quality 37000 could react with the antibody of FedF and SLT-IIeB.
  
  
  CONCLUSIONS
  The attenuated Salmonella choleraesuis vaccine strain could probably serve as a vaccine against edema disease and piglets paratyphoid.
authors.affiliation: State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan 430070, China. manxiaoying2002@yahoo.com.cn
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Man
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Teng
authors.initials: T
authors.lastname: Yu
authors.forename: Zhanqin
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yindi
authors.initials: Y
authors.lastname: Xu
authors.forename: Aizhen
authors.initials: A
authors.lastname: Guo
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012475:Salmonella / Q000235:genetics* / Q000276:immunology*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000235:genetics* / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D014613:Vaccines, Attenuated