title: HAT-HDAC interplay modulates global histone H3K14 acetylation in gene-coding regions during stress.
abstract: Histone acetylation and deacetylation are important for gene regulation. The histone acetyltransferase, Gcn5, is an activator of transcriptional initiation that is recruited to gene promoters. Here, we map genome-wide Gcn5 occupancy and histone H3K14ac at high resolution. Gcn5 is predominantly localized to coding regions of highly transcribed genes, where it collaborates antagonistically with the class-II histone deacetylase, Clr3, to modulate H3K14ac levels and transcriptional elongation. An interplay between Gcn5 and Clr3 is crucial for the regulation of many stress-response genes. Our findings suggest a new role for Gcn5 during transcriptional elongation, in addition to its known role in transcriptional initiation.
authors.affiliation: Department of Biosciences and Medical Nutrition, Karolinska Institute, S-141 57 Huddinge, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Johnsson
authors.forename: Mickaël
authors.initials: M
authors.lastname: Durand-Dubief
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Xue-Franzén
authors.forename: Michelle
authors.initials: M
authors.lastname: Rönnerblad
authors.forename: Karl
authors.initials: K
authors.lastname: Ekwall
authors.forename: Anthony
authors.initials: A
authors.lastname: Wright
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones; D016366:Open Reading Frames; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014158:Transcription, Genetic; D000099224:p300-CBP-Associated Factor
chemical_list: D000123:Acetyltransferases; D018797:Cell Cycle Proteins; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins; C115302:Clr3 protein, S pombe; C501322:Gcn5 protein, S pombe; D000099224:p300-CBP-Associated Factor