title: Soy isoflavones mitigate long-term femoral and lumbar vertebral bone loss in middle-aged ovariectomized mice.
abstract: We evaluated the protective effects of soy isoflavones (SIF) against osteoporosis in middle-aged ovariectomized (OVX) mice. SIF (30 mg/kg or 60 mg/kg) or 17beta-estradiol (E(2)) was administered to OVX mice for 4 months after bilateral ovariectomy. We observed the biochemical markers of bone turnover, e.g., alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), in serum. We also observed the bone mineral density (BMD) in femurs and lumbar vertebrae. In addition, we examined trabecular bone and interstitial cells in the femur using hematoxylin and eosin staining. The decrease in ALP levels and the increase in TRAP levels normally resulting from ovariectomy were suppressed by administration of 60 mg/kg SIF or E(2). Administration of 60 mg/kg SIF or E(2) also maintained the BMD, trabecular bone, and interstitial cells in OVX mice compared to those in pre-OVX mice. These results suggest that 60 mg/kg SIF effectively mitigates ovariectomy-induced osteoporosis in middle-aged mice.
authors.affiliation: Research Center, Natural F&P Corp., Hallym University, Chuncheon, Republic of Korea.
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Yoon-Bok
authors.initials: YB
authors.lastname: Lee
authors.forename: Keun-Ha
authors.initials: KH
authors.lastname: Lee
authors.forename: Heon-Soo
authors.initials: HS
authors.lastname: Sohn
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Kyung-Hwan
authors.initials: KH
authors.lastname: Cho
authors.forename: Yong Kook
authors.initials: YK
authors.lastname: Shin
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Kim
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000375:Aging / Q000187:drug effects / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D050071:Bone Density Conservation Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004958:Estradiol / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D005269:Femur / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D008159:Lumbar Vertebrae / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D010024:Osteoporosis / Q000097:blood / Q000517:prevention & control*; D010052:Ovariectomy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D013025:Glycine max / Q000737:chemistry*
chemical_list: D050071:Bone Density Conservation Agents; D007529:Isoflavones; D010936:Plant Extracts; D004958:Estradiol; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase