Response of human bone marrow stromal cells to a resorbable P(2)O(5)-SiO(2)-CaO-MgO-Na(2)O-K(2)O phosphate glass ceramic for tissue engineering applications.
This work focuses on the synthesis and characterization of a novel bioresorbable glass ceramic phosphate-based material (GC-ICEL). More specifically, its solubility in different aqueous media (water, Tris-HCl and acellular simulated body fluid) and the response of human stromal cells cultured on it were investigated. X-ray diffraction analysis showed the presence of two crystalline phases identified as Na(2)Mg(PO(4))(3) and Ca(2)P(2)O(7) and dissolution tests highlighted a preferential dissolution of the Na(2)Mg(PO(4))(3) phase and of the residual amorphous phase in all the chosen media. Soaking tests in simulated body fluid showed precipitation of a hydroxyapatite layer, demonstrating the bioactivity of GC-ICEL, which is partially due to the reported bioactivity of Ca(2)P(2)O(7). The effect of GC-ICEL on adhesion, proliferation and osteoblastic gene expression of human bone marrow-derived stromal cells was also studied. Combining molecular and biochemical analyses, it was found that bone marrow cell differentiation was stimulated over proliferation on GC-ICEL. Moreover, the expression of bone-related genes in cells cultured on GC-ICEL confirmed the bioactivity of this phosphate-based glass ceramic, which might have a stimulatory effect on osteogenesis.
Leonardi
E
E
Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, Bologna, Italy.
Ciapetti
G
G
Baldini
N
N
Novajra
G
G
Verné
E
E
Baino
F
F
Vitale-Brovarone
C
C
D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002516:Ceramics; D017931:DNA Primers; D005898:Glass; D006801:Humans; D017154:Stromal Cells / Q000737:chemistry*; D023822:Tissue Engineering; D014961:X-Ray Diffraction
D017931:DNA Primers