Effect of chlorotriphenyl derivatives of Sn and Pb upon biophysical properties of membranes.
Biophysical activity of two twin organometallic compounds Triphenyltin chloride (TPhT) and Triphenyllead chloride (TPhL) in their interreaction with model membranes, as well as with yeast cells Saccharomyces cerevisiae, was investigated. Four measurement methods were used in the experiments: two physical methods (spin probes method and the electric method); two biological methods (minimal inhibitory concentration /MIC/ and yeast survival test). It has been found that the activity of TPhT in interaction with model membranes and yeast cells is distinctly greater than that of TPhL. The activity manifests itself by considerable increase in the fluidity of the middle part of liposome bilayer, change in the polarization direction of the transmembrane voltage of filters impregnated with lauric acid, and in complete inhibition of growth of yeast cells at the concentration of 5 microg/mL.
Man
Dariusz
D
Institute of Physics, Opole University, Oleska 48, 45-052 Opole, Poland.
Podolak
Marian
M
Engel
Grzegorz
G
Boniewska
Ewa
E
D055592:Biophysical Phenomena; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D004578:Electron Spin Resonance Spectroscopy; D007850:Lauric Acids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009942:Organometallic Compounds / Q000494:pharmacology*; D009947:Organotin Compounds / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D013997:Time Factors
D007850:Lauric Acids; D008081:Liposomes; D009942:Organometallic Compounds; D009947:Organotin Compounds; C420974:triphenyllead chloride; C030358:lauric acid; C012594:triphenyltin chloride