Backbone assignment of the UHM domain of Puf60 free and bound to five ligands.
U2AF homology motifs (UHM) are protein domains that bind peptidic UHM ligand motifs (ULM) and thus form an intricate network of interactions involved in splicing regulation. Here, we report the backbone assignment of the UHM domain of the splicing factor Puf60 as well as (1)H, (15)N chemical shifts upon binding of the ULM peptides U2AF(65) (85-112), SF1 (1-25), SF3b155 (194-229), SF3b155 (317-357), and Prp16 (201-238).
Corsini
Lorenzo
L
Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstr. 1, Heidelberg, D-69117, Germany.
Sattler
Michael
M
D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009587:Nitrogen Isotopes / Q000737:chemistry; D009687:Nuclear Proteins; D017434:Protein Structure, Tertiary; D011522:Protons; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000737:chemistry*; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF
D002247:Carbon Isotopes; D002352:Carrier Proteins; D009587:Nitrogen Isotopes; D009687:Nuclear Proteins; D011522:Protons; D017413:Ribonucleoprotein, U2 Small Nuclear; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF