[Comparison of two kinds of cationic vectors-mediated gene delivery].
In order to study the important factors involved in cationic liposome-mediated gene transfer, Lipofectamine 2000 or DOTAP was evaluated using three types of cells (Hep-2, MCF-7 and SW-480) in vitro transfection efficiencies. Different properties of the two reagents were analyzed and compared by DNA arrearage assay and MTT assay. Both Lipofectamine 2000 and DOTAP had strong capability to combine with DNA; Lipofectamine 2000 can get higher transfection efficiency of the three cells by using GFP as report gene, meanwhile, DOTAP can also get higher transfection efficiency against Hep-2 cell. However, DOTAP showed lower transfection efficiency against MCF-7 and SW-480 cell. On the other hand, the cytotoxicity assay showed that over 85% cell viability of MCF-7 cell could be achieved both by Lipofectamine 2000 and DOTAP under the optimal transfection condition. Relatively speaking, Lipofectamine 2000 has very high transfection efficiency in a broad range of cell lines, but because of the special selectivity of cell type on liposome, DOTAP also has a broad application prospect.
Zhi
De-fu
DF
College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
Wang
Bing
B
Cui
Shao-hui
SH
Yang
Bao-ling
BL
Zhao
Bu-diao
BD
Zhao
Yi-nan
YN
Jiang
Yun-xia
YX
Yu
Shi-jun
SJ
Zhang
Shu-biao
SB
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004247:DNA / Q000235:genetics; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry* / Q000633:toxicity; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000633:toxicity; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
D005229:Fatty Acids, Monounsaturated; D008055:Lipids; C086724:Lipofectamine; D000644:Quaternary Ammonium Compounds; D049452:Green Fluorescent Proteins; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane