Effect of mechanical stretching on expressions of muscle specific transcription factors MyoD, Myf-5, myogenin and MRF4 in proliferated myoblasts.
We examined expression of four important members of myogenic regulatory factors (MRFs) in the myoblasts both at mRNA and protein levels, which were subjected to mechanical stretching in in vitro condition. Our results showed that MyoD expression existed both in the stretch and in the control group at all time periods of the mechanical stimulus. Myf-5 expressed only at early stage of the stretch group. Although mRNA and protein expressions of myogenin and MRF4 were detected both in the stretch and in the control group at 12 h after the stretching, their expressions were only shown in the stretch group at 24 h after the mechanical stimulus. However, at 36 and 48 h, none of the MRFs examined except MyoD appeared in both groups. Our results suggest that the MRFs are up-regulated upon mechanical stimulus and each member plays a different major role for either proliferation or differentiation of the myoblasts.
Abe
S
S
Department of Anatomy, Tokyo Dental College, Mihama-ku, Chiba-City, Chiba, Japan. abesh@tdc.ac.jp
Rhee
S
S
Iwanuma
O
O
Hiroki
E
E
Yanagisawa
N
N
Sakiyama
K
K
Ide
Y
Y
D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation; D015870:Gene Expression; D051379:Mice; D008858:Microscopy, Phase-Contrast; D017570:MyoD Protein / Q000096:biosynthesis* / Q000235:genetics; D032446:Myoblasts / Q000166:cytology / Q000502:physiology*; D051787:Myogenic Regulatory Factor 5 / Q000096:biosynthesis* / Q000235:genetics; D018006:Myogenic Regulatory Factors / Q000096:biosynthesis* / Q000235:genetics; D018008:Myogenin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical
C497418:Myf5 protein, mouse; D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D012333:RNA, Messenger; C063403:myogenic factor 6