1H, 15N and 13C assignments of yellow fluorescent protein (YFP) Venus.
We present here the backbone and side-chain NMR assignments of YFP Venus, a 238-residue protein that emits yellow fluorescence in its native state. Venus is a variant of the green fluorescent protein (GFP), which has improved chromophore maturation and brightness, and the photochemistry and photophysics of which are insensitive to experimental conditions, such as the pH value and buffer content, making it a favourable biomarker.
Hsu
Shang-Te Danny
ST
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. stdh2@cam.ac.uk
Behrens
Caroline
C
Cabrita
Lisa D
LD
Dobson
Christopher M
CM
D000595:Amino Acid Sequence; D002247:Carbon Isotopes / Q000737:chemistry; D008164:Luminescent Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000737:chemistry; D011522:Protons
D002247:Carbon Isotopes; D008164:Luminescent Proteins; D009587:Nitrogen Isotopes; D011522:Protons