Evaluation and simplified measurement of infarct size by myocardial contrast echocardiography in a rat model of myocardial infarction.
To test the feasibility and accuracy of myocardial contrast echocardiography (MCE) for predicting infarct size (IS) in a rat model of myocardial infarction (MI) and to compare a simplified single plane-based measurement of IS with the conventional three plane-based approach. Fifty male SD rats underwent left anterior descending artery ligation and were evaluated by MCE 8 h post MI. IS was calculated by the single and three plane-based approaches, compared to that determined by triphenyltetrazolium chloride (TTC) staining method. Simplified single plane-based MCE approach and TTC method showed similar IS values (38.48 +/- 16.80% vs. 35.72 +/- 15.33%, P > 0.05) and presented a favorable positive correlation (r = 0.851, P < 0.001). IS values derived from simplified single plane-based approach was also highly significantly correlated with that by the conventional MCE method (r = 0.973, P < 0.001). Bland-Altman plots also displayed satisfactory agreement between them. MCE was validated as a novel technique to quantify infarct area in rats with MI. A single measurement at the mid-papillary muscle level may become a simple, efficient and reliable approach for in vivo IS assessment.
Chen
Xianghui
X
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Cui
Kai
K
Xiu
Jiancheng
J
Lin
Huanbing
H
Lao
Yi
Y
Zhou
Biying
B
Liang
Feixue
F
Zha
Daogang
D
Bin
Jianping
J
Liu
Yili
Y
D000818:Animals; D004396:Coloring Agents; D003287:Contrast Media; D004195:Disease Models, Animal; D004452:Echocardiography; D005240:Feasibility Studies; D008297:Male; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D009206:Myocardium / Q000473:pathology*; D011237:Predictive Value of Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods; D013778:Tetrazolium Salts
D004396:Coloring Agents; D003287:Contrast Media; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium