title: Simultaneous analysis of catechol-O-methyl transferase activity, S-adenosylhomocysteine and adenosine.
abstract: Novel HPLC method utilizing UV-detection was developed to analyse catechol-O-methyltransferase (COMT) products, vanillic acid and isovanillic acid, S-adenosylhomocysteine (SAH) and adenosine formed from dihydroxybenzoic acid and S-adenosyl-L-methionine (SAM) by incubation of the rat tissues. Entacapone, a COMT inhibitor, prevented the formation of SAH only partially in the striatal homogenate whereas in the kidney homogenate the increase of SAH was prevented by entacapone. In conclusion, this method was reliable, rapid and simple. COMT seemed to be partially responsible on the SAM utilizing methylations in the striatal homogenates while in the high COMT activity tissue, COMT was the main SAH producing methyltransferase.
authors.affiliation: Institute of Biomedicine, Pharmacology, Biomedicum Helsinki, University of Helsinki, Finland. ilkka.reenila@helsinki.fi
authors.forename: Ilkka
authors.initials: I
authors.lastname: Reenilä
authors.forename: Pekka
authors.initials: P
authors.lastname: Rauhala
mesh_terms: D000241:Adenosine / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002394:Catechol O-Methyltransferase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012435:S-Adenosylhomocysteine / Q000032:analysis / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism; D014641:Vanillic Acid / Q000032:analysis / Q000378:metabolism
chemical_list: D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D002394:Catechol O-Methyltransferase; D014641:Vanillic Acid; D000241:Adenosine