Cross-species amplification and polymorphism of microsatellite loci in the soybean aphid, Aphis glycines.
We tested the utility of 18 previously characterized Aphis spp. microsatellite loci for polymorphism and differentiation among populations of the soybean aphid, Aphis glycines. Loci were chosen from a closely related species (Aphis gossypii) and a more distantly related species (Aphis fabae). We found nine loci to be polymorphic among Korean and North American populations. Overall expected heterozygosity was moderate (average: 0.47; range: 0-1), although populations substantially differed in deviations from Hardy-Weinberg equilibrium. These loci will be valuable in characterizing population differentiation, migration and adaptation in an economically important pest of soybeans.
Michel
Andrew P
AP
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, USA. michel.70@osu.edu
Zhang
Wei
W
Jung
Jin Kyo
JK
Kang
Sung-Taeg
ST
Mian
M A Rouf
MA
D000818:Animals; D001042:Aphids / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005828:Genetics, Population; D007723:Korea; D018895:Microsatellite Repeats / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D013025:Glycine max / Q000469:parasitology*; D013045:Species Specificity; D014481:United States
D017931:DNA Primers