title: Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing.
abstract: The purpose of the present study is to develop a novel method for the fabrication of transferable micropatterned cell sheets for tissue engineering. To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primary rat hepatocytes were seeded on the dish surfaces printed with fibronectin. Under serum-free conditions, hepatocytes were attached onto fibronectin domains selectively. Then, a second cell type of endothelial cells was seeded in the presence of serum. Double fluorescent staining revealed that endothelial cells successfully adhered to the intervals of hepatocyte domains. Finally, all the cells were harvested as a single contiguous micropatterned cell sheet upon temperature-reduction. With a cell sheet manipulator having a gelatin layer for the support of harvested cell sheets, harvested micropatterned cell sheets were transferred to new dish surfaces. This technique would be useful for the fabrication of thick tissue constructs having a complex microarchitecture.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Imen
authors.initials: I
authors.lastname: Elloumi Hannachi
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Itoga
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kumashiro
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology; D008422:Materials Testing; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials