title: Virus assembly occurs following a pH- or Ca2+-triggered switch in the thermodynamic attraction between structural protein capsomeres.
abstract: Viral self-assembly is of tremendous virological and biomedical importance. Although theoretical and crystallographic considerations suggest that controlled conformational change is a fundamental regulatory mechanism in viral assembly, direct proof that switching alters the thermodynamic attraction of self-assembling components has not been provided. Using the VP1 protein of polyomavirus, we report a new method to quantitatively measure molecular interactions under conditions of rapid protein self-assembly. We show, for the first time, that triggering virus capsid assembly through biologically relevant changes in Ca(2+) concentration, or pH, is associated with a dramatic increase in the strength of protein molecular attraction as quantified by the second virial coefficient (B(22)). B(22) decreases from -2.3 x 10(-4) mol ml g(-2) (weak protein-protein attraction) to -2.4 x 10(-3) mol ml g(-2) (strong protein attraction) for metastable and Ca(2+)-triggered self-assembling capsomeres, respectively. An assembly-deficient mutant (VP1CDelta63) is conversely characterized by weak protein-protein repulsion independently of chemical change sufficient to cause VP1 assembly. Concomitant switching of both VP1 assembly and thermodynamic attraction was also achieved by in vitro changes in ammonium sulphate concentration, consistent with protein salting-out behaviour. The methods and findings reported here provide new insight into viral assembly, potentially facilitating the development of new antivirals and vaccines, and will open the way to a more fundamental physico-chemical description of complex protein self-assembly systems.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Yap P
authors.initials: YP
authors.lastname: Chuan
authors.forename: Yuan Y
authors.initials: YY
authors.lastname: Fan
authors.forename: Linda H L
authors.initials: LH
authors.lastname: Lua
authors.forename: Anton P J
authors.initials: AP
authors.lastname: Middelberg
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D011120:Polyomavirus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D017434:Protein Structure, Tertiary; D013816:Thermodynamics; D019065:Virus Assembly / Q000502:physiology*
chemical_list: D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus; D000645:Ammonium Sulfate; D002118:Calcium