Stimulatory effect of apigenin-6-C-beta-L-fucopyranoside on insulin secretion and glycogen synthesis.
In vivo and in vitro treatments were carried out to investigate the effects of apigenin-6-C-beta-L-fucopyranoside (1), isolated from Averrhoa carambola L. (Oxalidaceae), on serum glucose and insulin levels in hyperglycemic rats as well as its effect on glycogen synthesis in normal rat soleus muscle. Apigenin-6-C-beta-L-fucopyranoside showed an acute effect on blood glucose lowering in hyperglycemic rats and stimulated glucose-induced insulin secretion. A stimulatory effect of 1 on glycogen synthesis was observed when muscles were incubated with this flavonoid and also its effect was completely nullified by pre-treatment with insulin signal transduction inhibitors. Taking this into account, the MAPK-PP1 and PI3K-GSK3 pathways are involved in the apigenin-6-C-beta-L-fucopyranoside-induced increase in glycogen synthesis in muscle. This study provides evidence for dual effects of apigenin-6-C-beta-L-fucopyranoside as an antihyperglycemic (insulin secretion) as well as an insulinomimetic (glycogen synthesis) agent.
Cazarolli
Luisa Helena
LH
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Florianópolis, SC, Brazil.
Folador
Poliane
P
Moresco
Henrique Hunger
HH
Brighente
Inês Maria Costa
IM
Pizzolatti
Moacir Geraldo
MG
Silva
Fátima Regina M Barreto
FR
D000818:Animals; D047310:Apigenin / Q000302:isolation & purification / Q000627:therapeutic use*; D029624:Ferns / Q000737:chemistry*; D006003:Glycogen / Q000378:metabolism*; D006027:Glycosides / Q000302:isolation & purification / Q000627:therapeutic use*; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D007328:Insulin / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D006027:Glycosides; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; C546678:apigenin-6-C-beta-L-fucopyranoside; D047310:Apigenin; D006003:Glycogen