title: A model of sequential branching in hierarchical cell fate determination.
abstract: Multipotent stem or progenitor cells undergo a sequential series of binary fate decisions, which ultimately generate the diversity of differentiated cells. Efforts to understand cell fate control have focused on simple gene regulatory circuits that predict the presence of multiple stable states, bifurcations and switch-like transitions. However, existing gene network models do not explain more complex properties of cell fate dynamics such as the hierarchical branching of developmental paths. Here, we construct a generic minimal model of the genetic regulatory network controlling cell fate determination, which exhibits five elementary characteristics of cell differentiation: stability, directionality, branching, exclusivity, and promiscuous expression. We argue that a modular architecture comprising repeated network elements reproduces these features of differentiation by sequentially repressing selected modules and hence restricting the dynamics to lower dimensional subspaces of the high-dimensional state space. We implement our model both with ordinary differential equations (ODEs), to explore the role of bifurcations in producing the one-way character of differentiation, and with stochastic differential equations (SDEs), to demonstrate the effect of noise on the system. We further argue that binary cell fate decisions are prevalent in cell differentiation due to general features of the underlying dynamical system. This minimal model makes testable predictions about the structural basis for directional, discrete and diversifying cell phenotype development and thus can guide the evaluation of real gene regulatory networks that govern differentiation.
authors.affiliation: Institute for Biocomplexity and Informatics, University of Calgary, Calgary, Alberta, Canada T2N 1N4. dfoster@ucalgary.ca
authors.forename: David V
authors.initials: DV
authors.lastname: Foster
authors.forename: Jacob G
authors.initials: JG
authors.lastname: Foster
authors.forename: Sui
authors.initials: S
authors.lastname: Huang
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Kauffman
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008957:Models, Genetic; D039902:Multipotent Stem Cells / Q000166:cytology*; D013269:Stochastic Processes