title: Comparison of osteopontin, beta-catenin and hnRNP B1 expression in lung carcinomas.
abstract: This study was performed to compare osteopontin (OPN), beta-catenin and heterogeneous nuclear ribonucleoprotein B1 (hnRNP B1) immunreactivities in small cell lung carcinomas (SCLC) and non-small cell lung carcinomas (NSCLC). Correlation of these three antibodies with grade and clinicopathologic stage of the tumor in NSCLC was also investigated. Twenty-nine SCLC, 6 large cell carcinoma, 36 adenocarcinoma and 30 squamous cell carcinoma (SCC), totally 101 cases, were included in this study. OPN, beta-catenin and hnRNP B1 expressions were immunohistochemically evaluated. OPN positivity was 6.9% in SCLC and 67% in NSCLC. When NSCLC types were individually considered, OPN positivity was 66.7% in large cell carcinoma, 80% in SCC and 55.6% in adenocarcinomas. beta-catenin positivity was observed in 48.6% of NSCLC and none of SCLC cases. These results were statistically significant (p < 0.05). Neither grade nor stage of NSCLC was correlated with osteopontin, beta-catenin or hnRNP B1 immunreactivity. We observed that OPN and beta-catenin are useful in differentiating SCLC from NSCLC. This may be helpful in small lung biopsies where morphology is obscured by crush artifacts.
authors.affiliation: Department of Pathology, Harran University Faculty of Medicine, 63100, Sanliurfa, Turkey. sevimemin@mynet.com
authors.forename: Muhammet Emin
authors.initials: ME
authors.lastname: Guldur
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Kibar
authors.forename: Hale
authors.initials: H
authors.lastname: Deniz
authors.forename: Kemal
authors.initials: K
authors.lastname: Bakir
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D053495:Osteopontin / Q000096:biosynthesis*; D051176:beta Catenin / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D051176:beta Catenin; C439768:hnRNP A2; D053495:Osteopontin