title: Performance of the RealStar Chikungunya virus real-time reverse transcription-PCR kit.
abstract: A novel commercial Chikungunya virus real-time reverse transcription-PCR (RT-PCR) kit was evaluated on a comprehensive panel of original patient samples. The assay was 100% sensitive and specific in comparison to a published real-time RT-PCR. Viral loads from both assays were highly correlated. The kit proved to be suitable for routine use in patient care.
authors.affiliation: Institute of Virology, University of Bonn Medical Centre, Sigmund Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Panning
authors.forename: Markus
authors.initials: M
authors.lastname: Hess
authors.forename: Waldemar
authors.initials: W
authors.lastname: Fischer
authors.forename: Klaus
authors.initials: K
authors.lastname: Grywna
authors.forename: Martin
authors.initials: M
authors.lastname: Pfeffer
authors.forename: Christian
authors.initials: C
authors.lastname: Drosten
mesh_terms: D018354:Alphavirus Infections / Q000175:diagnosis*; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019562:Viral Load
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic