title: Phytochemical, antibacterial, antiproliferative, and antioxidant potentials and DNA damage-protecting activity of Acacia salicina extracts.
abstract: The extract enriched in total oligomer flavonoids (TOF), and the aqueous, methanol, and ethyl acetate extracts of Acacia salicina were investigated for their polyphenolic compound content, antioxidative activity in the nitro blue tetrazolium/riboflavin assay system, antibacterial activity against Gram-positive and Gram-negative bacterial reference strains, antigenotoxic activity tested with the Ames assay, and cytotoxic activity against the K562 human chronic myelogenous leukemia cell line and L1210 leukemia murine cells. TOF extract was effective at inhibiting nitro blue tetrazolium reduction by superoxide radical in a nonenzymatic O(2)(*-)-generating system. Significant activity against bacterial reference strains Staphylococcus aureus, Enterococcus faecalis, Salmonella enteritidis, and Salmonella typhimurium was shown with all the tested extracts. These extracts significantly decreased the genotoxicity induced by sodium azide and 4-nitro-o-phenylenediamine. A pronounced cytotoxic effect on both leukemia cell lines was detected in TOF, methanolic and ethyl acetate extracts. The antioxidant, antimicrobial, antigenotoxic, and cytotoxic activities exhibited by A. salicina depended on the chemical composition of the tested extracts.
authors.affiliation: Unité de Pharmacognosie/Biologie Moléculaire/UR, Faculté de Pharmacie de Monastir, Tunisie.
authors.forename: Ines Bouhlel
authors.initials: IB
authors.lastname: Chatti
authors.forename: Ilef
authors.initials: I
authors.lastname: Limem
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani
authors.forename: Wissem
authors.initials: W
authors.lastname: Bhouri
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Sghaier
authors.forename: Aicha
authors.initials: A
authors.lastname: Nefatti
authors.forename: Hedi
authors.initials: H
authors.lastname: Ben Mansour
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016587:Antimutagenic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects; D002273:Carcinogens; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D010655:Phenylenediamines; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D059808:Polyphenols; D019810:Sodium Azide
chemical_list: D000900:Anti-Bacterial Agents; D016587:Antimutagenic Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D002273:Carcinogens; D005419:Flavonoids; D010636:Phenols; D010655:Phenylenediamines; D010936:Plant Extracts; D059808:Polyphenols; C012277:1,2-diamino-4-nitrobenzene; D019810:Sodium Azide