Identification of an antiamyloidogenic substance from mulberry leaves.
Fibrillar aggregates of amyloid beta-peptides are major constituents of the plaques found in the brains of patients with Alzheimer's disease, and have been implicated in the neurotoxicity of Alzheimer's. We previously reported that the methanol extract of mulberry leaves inhibits the formation of amyloid beta-peptide (1-42)-fibrils in vitro, and protects hippocampal neurons from amyloid beta-peptide (1-42)-induced cell death. In this study, we identified antiamyloidogenic substances, pheophorbide a, kaempferol -3-O-glucoside, and kaempferol -3-O-(6-malonyl) glucoside, from the methanol extract of mulberry leaves. We also compared the antiamyloidogenic activity of pheophorbide a with that of other porphyrin-related compounds.
Khaengkhan
Parinda
P
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Nishikaze
Yuki
Y
Niidome
Tetsuhiro
T
Kanaori
Kenji
K
Tajima
Kunihiko
K
Ichida
Masatoshi
M
Harada
Shigeharu
S
Sugimoto
Hachiro
H
Kamei
Kaeko
K
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D044949:Kaempferols / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D009005:Monosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D031625:Morus / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry; D055503:Protein Multimerization / Q000187:drug effects
D016229:Amyloid beta-Peptides; D005960:Glucosides; D044949:Kaempferols; D009005:Monosaccharides; D010446:Peptide Fragments; D010936:Plant Extracts; D011166:Porphyrins; C075222:amyloid beta-protein (1-42); C544651:kaempferol -3-O-(6-malonyl) glucoside; C511963:kaempferol-3-O-glucoside; D002734:Chlorophyll; C032623:pheophorbide a; D000432:Methanol