Impact of glutathione on the gene expression of exoY and exoS in Pseudomonas aeruginosa.
OBJECTIVE
To study the impact of GSH (glutathione) on the gene expression of exoY and exoS in Pseudomonas aeruginosa.


METHODS
We treated P. aeruginosa with BSO (buthionine sulfoximine) and DEM (diethylmaleate) to deplete GSH, or construct the P. aeruginosa mutant containing a lacZGm disrupted gshB (glutathione synthetase) gene by homologous recombination technology. The expression of exoY and exoS was determined by measuring light production of the lux-based reporters on pMS402.


RESULTS
The expression of exoY and exoS decreased in the gshB mutant and P. aeruginosa treated with BSO and DEM.


CONCLUSIONS
GSH in the P. aeruginosa can increase the expression of the genes exoY and exoS. Furthermore, this result provided possibilities to elucidate the molecular mechanisms of pathogenesis and immune response triggered by P. aeruginosa.
Zhang
Yani
Y
Faculty of Life Sciences, Northwest University, Xi' an 710069, China. yani@nwu.edu.cn
Wei
Yang
Y
D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects* / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000494:pharmacology*; D005981:Glutathione Synthase / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D037521:Virulence Factors; D019328:Buthionine Sulfoximine; D004247:DNA; C111504:ExoY protein, bacteria; D005964:Glucosyltransferases; D036002:ADP Ribose Transferases; C444820:exoenzyme S; D005981:Glutathione Synthase; D005978:Glutathione