Backbone NMR assignments of DFP-inhibited mature subtilisin E.
Here we report the backbone chemical shifts of the DFP-inhibited mature subtilisin E, which was uniformly labeled by (13)C, (15)N with a supplement of excess calcium.
Chen
Yu-Jen
YJ
Department of Biochemistry, Robert-Wood-Johnson Medical School, 675 Hoes Ln, Piscataway, NJ 08854, USA.
Wu
Kuen-Phon
KP
Kim
Seho
S
Falzon
Liliana
L
Inouye
Masayori
M
Baum
Jean
J
D000595:Amino Acid Sequence; D001407:Bacillus / Q000737:chemistry*; D002247:Carbon Isotopes / Q000737:chemistry; D007531:Isoflurophate / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009587:Nitrogen Isotopes / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011522:Protons; D013381:Subtilisins / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D007531:Isoflurophate; D013381:Subtilisins