Brain banking: making the most of your research specimens.
Unbiased stereology is a method for accurately and efficiently estimating the total neuron number (or other cell type) in a given area of interest(1). To achieve this goal 6-10 systematic sections should be probed covering the entire structure. Typically this involves processing 1/5 sections which leaves a significant amount of material unprocessed. In order to maximize the material, we propose an inexpensive method for preserving fixed tissue as part of a long-term storage research plan. As tissue is sliced and processed for the desired stain or antibody, alternate sections should be systematically placed in antigen preserve at -20 degrees C for future processing. Using 24-well plates, sections can be placed in order for future retrieval. Using this method, tissue can be stored and processed for immunohistochemistry over the course of years.
Burke
Mark W
MW
Department of Physiology, University of Montreal. mark.burke@umontreal.ca
Zangenehpour
Shahin
S
Ptito
Maurice
M
D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D002522:Chlorocebus aethiops; D008867:Microtomy / Q000379:methods; D009474:Neurons / Q000166:cytology; D013048:Specimen Handling / Q000379:methods*; D013238:Stereotaxic Techniques; D016707:Tissue Fixation / Q000379:methods