title: NMR assignment of the domain 513-651 from the SARS-CoV nonstructural protein nsp3.
abstract: Sequence-specific NMR assignments of an internal domain of the protein nsp3, nsp3(513-651), which is a part of the SARS coronavirus (SARS-CoV) replicase polyprotein, have been determined, using triple-resonance NMR experiments with the uniformly [(13)C,(15)N]-labeled protein. The complete assignments (>99%) provide the basis for the ongoing three-dimensional structure determination.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Amarnath
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Johnson
authors.forename: Pedro
authors.initials: P
authors.lastname: Serrano
authors.forename: Bill
authors.initials: B
authors.lastname: Pedrini
authors.forename: Kurt
authors.initials: K
authors.lastname: Wüthrich
mesh_terms: D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008970:Molecular Weight; D017434:Protein Structure, Tertiary; D011522:Protons; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
chemical_list: D011522:Protons; D017361:Viral Nonstructural Proteins; C510959:Nsp3 protein, SARS-CoV; D012324:RNA-Dependent RNA Polymerase