title: Advantages of the high resolution melting in the detection of BRCA1 or BRCA2 mutation carriers.
abstract: OBJECTIVE
  The aim of the study is to explore the reliability of the high resolution melting (HRM) analysis for the identification of BRCA1/BRCA2 mutation carriers among the family members of index patient (IP) and for distinguishing the presence of two or more genetic variants within the same amplicon.
  
  
  METHODS
  We studied 27 different BRCA1/BRCA2 pathogenic mutations detected in 35 families with 194 subjects. HRM was performed in the LightCycler 480 (Roche).
  
  
  RESULTS
  HRM method detected 110 BRCA1/BRCA2 mutations among the 192 relatives studied (57%). No false negative results were observed in any of the family members and all of them were in agreement with sequencing analysis, therefore the method might help to avoid unnecessary sequencing of wild type (WT) genotypes. The HRM method also allows the detection of other alterations that we initially had not searched (three unclassified variants and several polymorphisms). Furthermore, HRM has also been capable of distinguishing the presence of two or more genetic variants in the same amplicon of the same sample.
  
  
  CONCLUSIONS
  HRM is a rapid, sensitive, specific, cost-effective and reliable screening method that in less than 2 h allows the easy identification of BRCA1 and BRCA2 genetic variations and also avoids the unnecessary sequencing of WT genotypes. Furthermore the method is also capable of detecting new genetic variants and allows the simultaneous detection of the presence of more than one genetic variant.
authors.affiliation: Laboratory of Molecular Biology, Service of Clinical Analysis, University Hospital La Fe, Valencia, Spain.
authors.forename: Inmaculada de Juan
authors.initials: Ide J
authors.lastname: Jiménez
authors.forename: Eva
authors.initials: E
authors.lastname: Esteban Cardeñosa
authors.forename: Sarai
authors.initials: S
authors.lastname: Palanca Suela
authors.forename: Eva Barragán
authors.initials: EB
authors.lastname: González
authors.forename: Pascual
authors.initials: P
authors.lastname: Bolufer Gilabert
mesh_terms: D004252:DNA Mutational Analysis / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D019398:Genes, BRCA1; D024522:Genes, BRCA2; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000191:economics / Q000379:methods; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D009154:Mutation; D009691:Nucleic Acid Denaturation