[Effects of Simvastatin on osteoblast activity of human periodontal ligament cells].
OBJECTIVE
To study the effects of Simvastatin on the osteoblast activity of human periodontal ligament (PDL) cells.


METHODS
The third passage human PDL were cultured in conditional mineralization medium with different concentrations of Simvastatin. The cells were divided into A group (0 mol/L), B group (1 x 10(-9) mol/L), C group (1 x 10(-8) mol/L), D group (1 x 10(-7) mol/L) and E group (1 x 10(-6) mol/L). Alkaline phosphatase (ALP) activity, osteopontin (OPN) and capability of mineralization were measured.


RESULTS
Differentiation osteoblast and mineralization of human PDL were improved in all treatment groups with different concentrations of Simvastatin (1 x 10(-9), 1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol/L). Compared with control group, statistically significant differences were found in 1 x 10(-8) mol/L, 1 x 10(-7) mol/L and 1 x 10(-6) mol/L groups (P<0.05). The maximum effect was observed at the concentration of 1 x 10(-7) mol/L.


CONCLUSIONS
Optimal concentration of Simvastatin can improve the osteoblastic activity of human PDL.
Hu
Fei
F
Dept. of Orthodontics, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China.
Zhang
Xue-Yang
XY
Wang
Chun-Xian
CX
Zhou
Lei
L
D000469:Alkaline Phosphatase; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D010006:Osteoblasts; D010012:Osteogenesis; D053495:Osteopontin; D010513:Periodontal Ligament; D019821:Simvastatin
D053495:Osteopontin; D019821:Simvastatin; D000469:Alkaline Phosphatase