The physiological stress response and oxidative stress biomarkers in rainbow trout and brook trout from selenium-impacted streams in a coal mining region.
Selenium (Se) is an essential element that can be toxic at concentrations slightly greater than those required for homeostasis. The main chronic toxic effects of Se in fish are teratogenic deformities, but Se can also activate the physiological stress response and redox cycle with reduced glutathione causing oxidative damage. Rainbow trout, Oncorhynchus mykiss, appear to be more sensitive to Se than brook trout, Salvelinus fontinalis. The objective of this study was to compare the physiological stress response (plasma cortisol, glucose, triiodothyronine, thyroxine, gill Na+/K+ ATPase, cortisol secretory capacity, K and liver somatic index) and oxidative stress biomarkers (liver GSH, GPx, lipid peroxidation, vitamin A and vitamin E) in rainbow trout (RNTR) and brook trout (BKTR) collected from reference and Se-exposed streams. The physiological stress response was not impaired (cortisol secretory capacity unchanged); although there were species differences in plasma cortisol and plasma glucose levels. Liver GSH, GPx and vitamin levels were higher in RNTR than BKTR, but lipid peroxidation levels were not different. The elevated GSH reserves may make RNTR more sensitive to Se-induced lipid peroxidation, but this may be offset by the RNTR's higher antioxidant (GPx and vitamin) levels. Species-specific biochemical differences may mediate differences in Se sensitivity and be used in aquatic Se risk assessments.
Miller
L L
LL
Department of Biological Sciences, Water and Environmental Science Building, University of Lethbridge, 4401 University Drive, Lethbridge, Alberta, Canada. lana.miller@uleth.ca
Rasmussen
J B
JB
Palace
V P
VP
Hontela
A
A
D000416:Alberta; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D003032:Coal Mining; D005260:Female; D007220:Industrial Waste / Q000009:adverse effects*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000737:chemistry; D017686:Oncorhynchus mykiss / Q000097:blood / Q000378:metabolism / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects*; D045483:Rivers / Q000737:chemistry; D012643:Selenium / Q000032:analysis / Q000633:toxicity*; D012727:Sex Characteristics; D013045:Species Specificity; D013312:Stress, Physiological / Q000187:drug effects*; D013961:Thyroid Gland / Q000378:metabolism; D014337:Trout / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D012643:Selenium