New haemolytic potential assay on an alternative insect model.
A new screening test of a haemolytic potential is presented on Spodoptera larvae in vivo. The assay was evaluated using phenylhydrazine, the well-known xenobiotic which induces haemolysis in mammals. Haemocyte disintegration causes changes in total haemocyte counts and differentials. It is shown that only qualitative evaluation of panoptically stained haemolymph smears is sufficient, fast and cheap to detect haemolytic potential. The presented assay also prevents unneeded, non-ethical use of vertebrates.
Berger
Josef
J
Faculty of Health and Social Studies, University of South Bohemia, Emy Destinove, 37005 Ceske Budejovice, Czech Republic. berger@jcu.cz
D000826:Animal Testing Alternatives; D000818:Animals; D002452:Cell Count; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects; D006461:Hemolysis; D007814:Larva / Q000166:cytology; D023421:Models, Animal; D010659:Phenylhydrazines / Q000633:toxicity*; D018411:Spodoptera / Q000166:cytology*; D018675:Toxicity Tests / Q000379:methods*; D015262:Xenobiotics / Q000633:toxicity*
D010659:Phenylhydrazines; D015262:Xenobiotics; C030299:phenylhydrazine