[Assessment of glutathione peroxidase activity (GPX) in the diagnosis of diffuse pulmonary parenchymal changes].
DNA damage caused by free radicals is one of the mechanisms which are responsible for the occurrence of lung tumors, especially in case of cigarette smokers.Their tumors are radiologically often disseminated changes.


OBJECTIVE
To define the correlation between GPX activity in erythrocyte hemolysate and pulmonary parenchymal extract and the etiology of diffuse pulmonary parenchymal changes.


METHODS
The study group comprised 40 subjects classified to VTS due to diffuse pulmonary parenchymal changes. The control group included 40 clinically healthy subjects. In the examined group GPX activity in erythrocyte hemolysate and pulmonary parenchymal extract was marked. The material was 5.4 ml of vein blood taken one-time from all subjects and a sample of pulmonary parenchyma obtained during VTS or toracotomy in patients with pulmonary parenchymal changes.


RESULTS
Lower values of GPX activity in erythrocyte hemolysate and pulmonary parenchymal extract which are statistically significant were observed compared with the control group.


CONCLUSIONS
Higher GPX activity in erythrocyte hemolysate and healthy pulmonary parenchymal extract compared with pulmonary parenchymal changes can show the influence of GPX on the development of disease process. Higher GPX activity in erythrocte hemolysate and pulmonary parenchymal extract in the "sarcoidosis group" compared with the "lung carcinoma group" can be an additional marker in differential dignostics. The determination of GPX activity in erythrocyte hemolysate can be used as a supplementary laboratory test which will facilitate diagnosis of pulmonary parenchymal changes.
Santorek-Strumiłło
Edyta
E
Department of Chest Surgery, Oncologic and General Surgery, University Hospital No. 2, Medical University of Lodz. edysiaj@wp.pl
Brocki
Marian
M
Misiak
Piotr
P
Wcisło
Szymon
S
Kordiak
Jacek
J
D003937:Diagnosis, Differential; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000201:enzymology*; D017563:Lung Diseases, Interstitial / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology* / Q000473:pathology; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D014020:Tissue Extracts / Q000378:metabolism
D014020:Tissue Extracts; D005979:Glutathione Peroxidase