Biological markers in cerebrospinal fluid (CSF) and evaluation of in vitro effect of CSF on PC12 cell line viability in Alzheimer's disease.
The definite diagnosis of Alzheimer's disease (AD) is based on post mortem pathological examination. To date, there is no laboratory test that can discriminate AD patients from healthy individuals. In the perspective of recent knowledge, there are three cerebrospinal fluid (CSF) markers which have the highest sensitivity and specificity: A beta(1-40), A beta(1-42), and p-tau. In the present study, 15 'Probable Alzheimer's Disease' (PAD) patients and 15 control subjects were included. PAD patients were selected from the patients of Dokuz Eylül University Neurology Department Dementia outpatient clinic and control subjects were selected from the patients who were undergone epidural anesthesia because of any surgical operation. The concentrations of Ab1-40, Ab1-42, and p-tau in CSF were quantified by using ELISA. Also, the effects of 'PAD patients' CSF on the survival of PC12 cell line were assessed. There was a significant decrease of Ab1-40 and increase of p-tau in patients with AD when compared with controls. Ab1-42 concentration was not significantly different between groups. There was a positive correlation between duration of the disease and CSF of p-tau concentration in patients with AD. There was no significant difference in cell line viability values between groups.
Yaka
Erdem
E
Department of Neurology, School of Medicine, Dokuz Eylül University, Izmir, Turkey. erdem.yaka@deu.edu.tr
Egrilmez
Mehtap Yuksel
MY
Keskinoglu
Pembe
P
Cavdar
Zahide
Z
Genc
Sermin
S
Genc
Kursad
K
Iyilikci
Leyla
L
Yener
Görsev G
GG
D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000633:toxicity; D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D002470:Cell Survival; D016009:Chi-Square Distribution; D003603:Cytotoxins / Q000134:cerebrospinal fluid* / Q000633:toxicity; D003704:Dementia; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016716:PC12 Cells; D010446:Peptide Fragments / Q000134:cerebrospinal fluid* / Q000633:toxicity; D012372:ROC Curve; D051381:Rats; D018709:Statistics, Nonparametric; D016875:tau Proteins / Q000134:cerebrospinal fluid* / Q000633:toxicity
D016229:Amyloid beta-Peptides; D015415:Biomarkers; D003603:Cytotoxins; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); D016875:tau Proteins