title: Evaluation of Leishmania species reactivity in human serologic diagnosis of leishmaniasis.
abstract: The sensitivities and specificities of IgG-ELISA and IgG flow cytometry based techniques using different Leishmania species were determined using sera collected from 40 cutaneous or visceral leishmaniasis patients. The flow cytometry technique, using promastigote parasite forms, performed better than total soluble extract IgG-ELISA. At the species level, the use of Leishmania amazonensis and Leishmania major as antigens in enzyme linked immunosorbent assay (ELISA) decreased the overall sensitivity. To assess the specificity of these tests, sera from malaria, toxoplasmosis, amoebiasis, schistosomiasis, and leprosy patients were used. We also included sera from Leishmania non-infected endemic individuals. The cutaneous species displayed a decreased specificity in both assays. Although more sensitive, flow cytometry using promastigote parasite forms generally presented lower levels of specificity when compared with total extract of IgG-ELISA. Overall, the results of the study show the potential of IgG flow cytometry for the diagnosis of leishmaniasis. Although highly sensitive, a refinement of the flow cytometry method should be performed to improve the overall specificity.
authors.affiliation: Parasite Disease Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal. rleal@ibmc.up.pt
authors.forename: Ricardo
authors.initials: R
authors.lastname: Silvestre
authors.forename: Nuno
authors.initials: N
authors.lastname: Santarém
authors.forename: Lúcia
authors.initials: L
authors.lastname: Teixeira
authors.forename: Joana
authors.initials: J
authors.lastname: Cunha
authors.forename: Henk
authors.initials: H
authors.lastname: Schallig
authors.forename: Anabela
authors.initials: A
authors.lastname: Cordeiro-da-Silva
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007891:Leishmania / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D007896:Leishmaniasis / Q000175:diagnosis* / Q000469:parasitology; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G