title: [Molecular interaction between PrP protein and the signal protein 14-3-3 beta].
abstract: The molecular interaction between PrP and 14-3-3 beta and the possible interactional domain between two proteins were studied by co-immunoprecipitation, pull down and FRET assays. The results showed that PrP protein could interact with 14-3-3 beta in vitro and in vivo. The domain which responded for the interaction was located at C-terminal of PrP (amino acid residues 106 to 126). This study of the interaction between PrP and 14-3-3 protein further provided the insight into the potential role of 14-3-3 in the biological function of PrP and the pathogenesis of prion disease.
authors.affiliation: Pathogenic Microorganisms Department, Medical School, Anhui University of Science and Technology, Huainan, 232001, China. 06mgy@163.com
authors.forename: Guo-yong
authors.initials: GY
authors.lastname: Mei
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Gui-rong
authors.initials: GR
authors.lastname: Wang
authors.forename: Bao-yun
authors.initials: BY
authors.lastname: Zhang
authors.forename: Chan
authors.initials: C
authors.lastname: Tian
authors.forename: Cao
authors.initials: C
authors.lastname: Chen
authors.forename: Rui-min
authors.initials: RM
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Li
authors.forename: Ke-xia
authors.initials: KX
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D006224:Cricetinae; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D011328:Prions / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits
chemical_list: D048948:14-3-3 Proteins; D011328:Prions