Transcriptome analyses revealed diverse expression changes in ago1 and hyl1 Arabidopsis mutants.
MicroRNAs (miRNAs) are 20-24 nucleotide endogenous regulatory molecules conserved in higher eukaryotes. In Arabidopsis, miRNAs are produced through step-wise cleavages of primary miRNA precursors (pri-miRNAs) by DICER-LIKE1 (DCL1). This cleavage step is also supported by a double-stranded RNA-binding protein, HYPONASTIC LEAVES1 (HYL1). In many cases, mature miRNA is predominantly incorporated into an endonuclease, ARGONAUTE1 (AGO1), which degrades miRNA-targeted mRNAs. Here, we examined and revealed whole genome transcriptomes in ago1-25 and hyl1-2 mutants using tiling arrays. The data in this paper are valuable for understanding the relationship between the miRNA pathway and its effect on transcriptomes.
Kurihara
Yukio
Y
Plant Genomic Network Research Team, Plant Functional Genomics Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan.
Kaminuma
Eli
E
Matsui
Akihiro
A
Kawashima
Makiko
M
Tanaka
Maho
M
Morosawa
Taeko
T
Ishida
Junko
J
Mochizuki
Yoshiki
Y
Shinozaki
Kazuo
K
Toyoda
Tetsuro
T
Seki
Motoaki
M
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060565:Argonaute Proteins; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D035683:MicroRNAs / Q000378:metabolism*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D018749:RNA, Plant / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
C117457:AGO1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; C420966:HYL1 protein, Arabidopsis; D035683:MicroRNAs; D018749:RNA, Plant; D016601:RNA-Binding Proteins