title: Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.
abstract: The CYP450 from Bacillus megaterium (BmCYP106A2) catalyzes the 15beta-hydroxylation of several steroids and also synthesizes mono-hydroxylated 9alpha- and 11alpha-OH-progesterone. This study reports on the ability of BmCYP106A2 to be efficiently reduced by the photosynthetic flavodoxin and, particularly, ferredoxin electron carriers from the cyanobacterium Anabaena. These results open the possibility for the design of a hybrid system to provide reducing equivalents for the hydroxylation process. Additionally, they suggest that despite the interaction of BmCYP106A2 with these proteins, particularly with flavodoxin, they do not rely on a precise complementarity of the reacting molecules, rearrangements might be required and alternative binding modes might contribute to the observed electron transfer reactions.
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: Guillermina
authors.initials: G
authors.lastname: Goñi
authors.forename: Andy
authors.initials: A
authors.lastname: Zöllner
authors.forename: Michael
authors.initials: M
authors.lastname: Lisurek
authors.forename: Adrián
authors.initials: A
authors.lastname: Velázquez-Campoy
authors.forename: Sira
authors.initials: S
authors.lastname: Pinto
authors.forename: Carlos
authors.initials: C
authors.lastname: Gómez-Moreno
authors.forename: Frank
authors.initials: F
authors.lastname: Hannemann
authors.forename: Rita
authors.initials: R
authors.lastname: Bernhardt
authors.forename: Milagros
authors.initials: M
authors.lastname: Medina
mesh_terms: D001410:Bacillus megaterium / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D000458:Cyanobacteria / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004579:Electron Transport; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism; D005418:Flavodoxin / Q000378:metabolism; D007700:Kinetics; D013250:Steroid Hydroxylases / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D001426:Bacterial Proteins; D045222:Electron Transport Chain Complex Proteins; D005288:Ferredoxins; D005418:Flavodoxin; D003577:Cytochrome P-450 Enzyme System; C517094:15beta-hydroxylase CYP106A2, Bacillus megaterium; D013250:Steroid Hydroxylases