Application of polyethyleneimine-modified scaffolds to the regeneration of cartilaginous tissue.
In this study, we analyzed the physicochemical and biophysical properties of three-dimensional scaffolds modified using polyethyleneimine (PEI) and applied these scaffolds to the cultivation of bovine knee chondrocytes (BKCs). PEI was crosslinked in the bulk or on the surface of the ternary scaffolds comprising polyethylene oxide, chitin and chitosan. The results revealed that when the concentration of PEI was less than 300 microg/mL, the cytotoxicity of a scaffold was on the same order in the two method of modification. An increase in the concentration of PEI favored the adhesion of BKCs. When the amount of PEI in scaffolds is fixed, the surface-modified scaffolds exhibited a higher adhesion efficiency of BKCs than the bulk-modified scaffolds. For the regeneration of cartilaginous components, a higher amount of PEI in a scaffold yielded larger amounts of proliferated BKCs, secreted glycosaminoglycans, and produced collagen. In addition, the formation of neocartilage in the surface-modified scaffolds was more effective than that in the bulk-modified scaffolds. These tissue-engineered scaffolds, modified by an appropriate concentration of PEI, can be potentially applied to cartilage repair in clinical trials.
Kuo
Yung-Chih
YC
Dept. of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China. chmyck@ccu.edu.tw
Ku
I-Nan
IN
D000704:Analysis of Variance; D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000254:growth & development*; D002417:Cattle; D002448:Cell Adhesion; D002470:Cell Survival; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006614:Hindlimb / Q000166:cytology; D007596:Joints / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D011094:Polyethyleneimine / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D006025:Glycosaminoglycans; D002686:Chitin; D011094:Polyethyleneimine; D048271:Chitosan