title: Flavobacterium tiangeerense sp. nov., a cold-living bacterium isolated from a glacier.
abstract: A novel cold-living, Gram-stain-negative, yellow-pigmented and obligately aerobic bacterium designated strain 0563(T) was isolated from the China No. 1 glacier. Phylogenetic analysis showed that strain 0563(T) was a member of the genus Flavobacterium, sharing the highest 16S rRNA gene sequence similarities with Flavobacterium limicola JCM 11473(T) (98.5 %) and Flavobacterium psychrolimnae NBRC 102679(T) (98.2 %). The genomic DNA G+C content was 34.8 mol%. Strain 0563(T) displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and iso-C(15 : 0), iso-C(15 : 0) 3-OH, summed feature 3 (comprising C(16 : 1)omega7c and/or C(16 : 1)omega6c), C(15 : 1)omega6c, iso-C(15 : 1) G and iso-C(16 : 0) 3-OH as the major fatty acids. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, the novel species Flavobacterium tiangeerense sp. nov. is proposed; the type strain is 0563(T) (=CGMCC 1.6847(T) =JCM 15087(T)).
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Xin
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Liang
authors.forename: De-Chao
authors.initials: DC
authors.lastname: Zhang
authors.forename: Hong-Can
authors.initials: HC
authors.lastname: Liu
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Ming-Shuang
authors.initials: MS
authors.lastname: Xu
authors.forename: Pei-Jin
authors.initials: PJ
authors.lastname: Zhou
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Zhou
mesh_terms: D002681:China; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005417:Flavobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S