Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay.
A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6)CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.
Qu
Qing
Q
Department of Infectious Disease Control, Beijing Institute of Disease Control and Prevention, Beijing 100071, China.
Zhu
Ziwen
Z
Wang
Yufei
Y
Zhong
Zhijun
Z
Zhao
Jin
J
Qiao
Feng
F
Du
Xinying
X
Wang
Zhoujia
Z
Yang
Ruifu
R
Huang
Liuyu
L
Yu
Yaqin
Y
Zhou
Lei
L
Chen
Zeliang
Z
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002002:Brucella / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity