title: Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses.
abstract: Soluble egg antigens of the parasitic helminth Schistosoma mansoni (S. mansoni egg antigen [SEA]) induce strong Th2 responses both in vitro and in vivo. However, the specific molecules that prime the development of Th2 responses have not been identified. We report that omega-1, a glycoprotein which is secreted from S. mansoni eggs and present in SEA, is capable of conditioning human monocyte-derived dendritic cells in vitro to drive T helper 2 (Th2) polarization with similar characteristics as whole SEA. Furthermore, using IL-4 dual reporter mice, we show that both natural and recombinant omega-1 alone are sufficient to generate Th2 responses in vivo, even in the absence of IL-4R signaling. Finally, omega-1-depleted SEA displays an impaired capacity for Th2 priming in vitro, but not in vivo, suggesting the existence of additional factors within SEA that can compensate for the omega-1-mediated effects. Collectively, we identify omega-1, a single component of SEA, as a potent inducer of Th2 responses.
authors.affiliation: Department of Parasitology, Leiden University Medical Centre, Leiden 2333 ZA, The Netherlands.
authors.forename: Bart
authors.initials: B
authors.lastname: Everts
authors.forename: Georgia
authors.initials: G
authors.lastname: Perona-Wright
authors.forename: Hermelijn H
authors.initials: HH
authors.lastname: Smits
authors.forename: Cornelis H
authors.initials: CH
authors.lastname: Hokke
authors.forename: Alwin J
authors.initials: AJ
authors.lastname: van der Ham
authors.forename: Colin M
authors.initials: CM
authors.lastname: Fitzsimmons
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Doenhoff
authors.forename: Jürgen
authors.initials: J
authors.lastname: van der Bosch
authors.forename: Katja
authors.initials: K
authors.lastname: Mohrs
authors.forename: Helmut
authors.initials: H
authors.lastname: Haas
authors.forename: Markus
authors.initials: M
authors.lastname: Mohrs
authors.forename: Maria
authors.initials: M
authors.lastname: Yazdanbakhsh
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schramm
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002454:Cell Differentiation; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D018345:Mice, Knockout; D010063:Ovum / Q000276:immunology; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D053662:Interleukin-4 Receptor alpha Subunit
chemical_list: D000947:Antigens, Helminth; D017077:Culture Media, Conditioned; D016207:Cytokines; D006023:Glycoproteins; D015801:Helminth Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C504002:Il4ra protein, mouse; D053662:Interleukin-4 Receptor alpha Subunit