Identification, cloning and sequencing of Escherichia coli strain chi1378 (O78:K80) iss gene isolated from poultry colibacillosis in Iran.
OBJECTIVE
To identify, clone and sequence the iss (increased serum survival) gene from E. coli strain chi1378 isolated from Iranian poultry and to predict its protein product, Iss.


RESULTS
The iss gene from E. coli strain chi1378 was amplified and cloned into the pTZ57R/T vector and sequenced. From the DNA sequence, the Iss predictive protein was evaluated using bioinformatics. Iss from strain chi1378 had 100% identity with other E. coli serotypes and isolates from different origins and also 98% identity with E. coli O157:H7 Iss protein. Phylogenetic analysis showed no significant different phylogenic groups among E. coli strains.


CONCLUSIONS
The strong association of predicted Iss protein among different E. coli strains suggests that it could be a good antigen to control and detect avian pathogenic E. coli (APEC).


CONCLUSIONS
Because the exact pathogenesis and the role of virulence factors are unknown, the Iss protein could be used as a target for vaccination in the future, but further research is required.
Derakhshandeh
A
A
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Zahraei Salehi
T
T
Tadjbakhsh
H
H
Karimi
V
V
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000382:microbiology*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D007492:Iran; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000382:microbiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C409210:Iss protein, E coli