title: Second harmonic generation microscopy to investigate collagen configuration: a pericarditis case study.
abstract: We have used second-harmonic-generation (SHG) to image collagen fibers in pericardial tissue removed from a patient with constrictive pericarditis and compared this to healthy pericardium. SHG imaging allowed for the visualization of collagen fibers without the need for staining or pretreatment. Images were compared to stained histology slides. Collagen fibers in SHG and histology images displayed the same structure and morphology. The mature collagen of the parietal pericardium was easily distinguishable from the new collagen accumulation due to the pericarditis. SHG imaging can provide a convenient and valuable architectural profile of collagen organization.
authors.affiliation: Department of Physics, McGill University, Montreal, Canada.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bélisle
authors.forename: Tiffany
authors.initials: T
authors.lastname: Zigras
authors.forename: Santiago
authors.initials: S
authors.lastname: Costantino
authors.forename: Raymond
authors.initials: R
authors.lastname: Cartier
authors.forename: Jagdish
authors.initials: J
authors.lastname: Butany
authors.forename: Paul W
authors.initials: PW
authors.lastname: Wiseman
authors.forename: Richard L
authors.initials: RL
authors.lastname: Leask
mesh_terms: D000328:Adult; D024022:Fibrillar Collagens / Q000737:chemistry / Q000648:ultrastructure*; D005583:Fourier Analysis; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods*; D010493:Pericarditis / Q000473:pathology* / Q000601:surgery; D010496:Pericardium / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D015203:Reproducibility of Results; D016896:Treatment Outcome
chemical_list: D024022:Fibrillar Collagens