title: Secondary repair of alveolar clefts using human mesenchymal stem cells.
abstract: Recently tissue engineering has become available as a regenerative treatment for bone defects; however, little has been reported on the application of tissue engineering for regeneration of cleft defect tissues. Mesenchymal-derived stem cells were applied to different kinds of bone substitute and compared in different animal models, but their usage in human critical defects remained unclear. In this study we report 2 patients with unilateral alveolar cleft, treated with the composite scaffold of demineralized bone mineral and calcium sulphate (Osteoset) loaded with mesenchymal stem cells (MSCs). Computed tomograms showed 34.5% regenerated bone, extending from the cleft walls and bridging the cleft after 4 months in one case and in the other there was 25.6% presentation of bone integrity. The available data revealed the conventional bone substitute was not a suitable scaffold for the MSC-induced bone regeneration.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Taleghani Hospital, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Behnia
authors.forename: Arash
authors.initials: A
authors.lastname: Khojasteh
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Azita
authors.initials: A
authors.lastname: Tehranchi
authors.forename: Ahad
authors.initials: A
authors.lastname: Khoshzaban
authors.forename: Saeed Hidari
authors.initials: SH
authors.lastname: Keshel
authors.forename: Reza
authors.initials: R
authors.lastname: Atashi
mesh_terms: D000293:Adolescent; D000539:Alveolar Process / Q000002:abnormalities / Q000000981:diagnostic imaging / Q000601:surgery*; D001672:Biocompatible Materials / Q000627:therapeutic use; D001857:Bone Matrix / Q000637:transplantation; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000627:therapeutic use; D002648:Child; D002971:Cleft Lip / Q000000981:diagnostic imaging / Q000601:surgery*; D002972:Cleft Palate / Q000000981:diagnostic imaging / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D016348:Osseointegration / Q000502:physiology; D011859:Radiography; D019651:Plastic Surgery Procedures / Q000379:methods; D012086:Reoperation; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes