Connecting viral with cellular interactomes.
Genome-scale screens for intraviral and virus-host protein interactions and the analysis of literature-curated datasets are able to provide a novel, comprehensive perspective of viruses, and virus-infected cells. Until now, large-scale interaction screens were predominantly performed with the yeast-two-hybrid (Y2H) system; however, alternative high-throughput technologies detecting binary protein interactions or protein complexes have been developed. Although many of the previous studies suffer from a rather poor validation of the results and few biological implications, these technologies potentially lead to a plethora of novel hypotheses. Here, we will give an overview of current approaches and their technical limitations, present recent examples and novel developments.
Bailer
S M
SM
Max-von-Pettenkofer Institut, Ludwig-Maximilians-Universität München, Muenchen, Germany. Bailer@mvp.uni-muenchen.de
Haas
J
J
D002477:Cells / Q000276:immunology / Q000821:virology; D020869:Gene Expression Profiling / Q000379:methods; D023281:Genomics / Q000379:methods; D054884:Host-Pathogen Interactions; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods; D049490:Systems Biology / Q000379:methods*; D020798:Two-Hybrid System Techniques; D014780:Viruses / Q000254:growth & development / Q000276:immunology