Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance.
The sterile insect technique (SIT) is a method of biological control whereby millions of factory reared sterile male insects are released into the field. This technique is commonly used to combat the Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae). Sterile medfly males are less competent in attracting and mating with wild females, a property commonly linked to the irradiation process responsible for the sterilization. As bacteria are important partners in the fly's life cycle, we used molecular analytical methods to study the community structure of the gut microbiota in irradiated male medflies. We find that the sterilizing irradiation procedure affects the gut bacterial community structure of the Mediterranean fruit fly. Although the Enterobacteriaceae family remains the dominant bacterial group present in the gut, the levels of Klebsiella species decreases significantly in the days after sterilization. In addition, we detected substantial differences in some bacterial species between the mass rearing strain Vienna 8 and the wild strain. Most notable among these are the increased levels of the potentially pathogenic species Pseudomonas in the industrial strain. Testing the hypothesis that regenerating the original microbiota community could result in enhanced competitiveness of the sterile flies, we found that the addition of the bacterial species Klebsiella oxytoca to the postirradiation diet enables colonization of these bacteria in the gut while resulting in decreased levels of the Pseudomonas sp. Feeding on diets containing bacteria significantly improved sterile male performance in copulatory tests. Further studies will determine the feasibility of bacterial amelioration in SIT operations.
Ben Ami
Eyal
E
Department of Plant Pathology and Microbiology, The Robert H Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel. eyal.benami@mail.huji.ac.il
Yuval
Boaz
B
Jurkevitch
Edouard
E
D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D044822:Biodiversity; D033622:Ceratitis capitata / Q000382:microbiology* / Q000502:physiology* / Q000528:radiation effects; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D041981:Gastrointestinal Tract / Q000382:microbiology* / Q000528:radiation effects; D041121:Klebsiella oxytoca / Q000254:growth & development; D008297:Male; D009691:Nucleic Acid Denaturation; D010572:Pest Control, Biological / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011549:Pseudomonas / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012098:Reproduction
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S