title: Intervention of Acidophilus-casei dahi and wheat bran against molecular alteration in colon carcinogenesis.
abstract: An in vivo trial was conducted on seventy five rats allocated to three groups, first group was DMH control group, second group was Wheat bran-DMH group (WB-DMH) in which wheat bran was given along with DMH (1,2-dimethylhydrazine) injection, third group was Wheat bran-DMH-Ac Dahi group in which both wheat bran and Acidophilus-casei dahi (a probiotic microorganisms fermented dairy product) was given along with DMH injections. Animals received subcutaneous injections of DMH at a dose rate of 20 mg/kg body weight, once weekly for 15 weeks. The rats were dissected at 40th week of experiment and comet assay was done in colonic cells to assess the DNA damage. The c-myc and cox-2 expression was studied in rat tumour. A significant reduction in DNA damage (48.2%) was observed in WB-DMH-Ac Dahi group as compared to DMH control group (87.8%). The c-myc and cox-2 mRNA level was found highest in DMH control group as compared to WB-DMH and WB-DMH-Ac Dahi group. The results of present study show the enhanced protective potential of Acidophilus-casei and wheat bran against DMH induced molecular alteration in colonic cells during carcinogenesis.
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Haryana, India. arvind00000@gmail.com
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.forename: Nikhlesh Kumar
authors.initials: NK
authors.lastname: Singh
authors.forename: Pushpalata Rabindra
authors.initials: PR
authors.lastname: Sinha
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
mesh_terms: D019813:1,2-Dimethylhydrazine; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000517:prevention & control*; D020552:Comet Assay; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004043:Dietary Fiber / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007778:Lactobacillus / Q000187:drug effects / Q000502:physiology*; D009153:Mutagens / Q000633:toxicity; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015014:Yogurt / Q000382:microbiology*
chemical_list: D004043:Dietary Fiber; D009153:Mutagens; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D019813:1,2-Dimethylhydrazine