Antibodies to ganglioside complexes consisting of asialo-GM1 and GQ1b or GT1a in Fisher and Guillain-Barré syndromes.
To determine the epitopes of ganglioside complexes (GSCs) containing GQ1b or GT1a, we investigated their reactivity to GSCs consisting of asialo-GM1 (GA1) and GQ1b or GT1a using IgG anti-GQ1b- or anti-GT1a-positive sera. Nine anti-GQ1b-positive sera had higher activity to GA1/GQ1b than to GQ1b, only five of which reacted with GM1/GQ1b and GD1b/GQ1b. Five of 14 sera positive for GA1/GT1a and GM1/GT1a were negative for GA1/GQ1b and GM1/GQ1b. Sialic acids attached to the internal galactose of gangliotetraose can influence the reactivity of anti-GSC antibodies. Screening for antibodies to GSCs containing GA1 is useful for elucidation of the antibody-mediated pathophysiology.
Ogawa
Go
G
Division of Neurology, Department of Internal Medicine 3, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
Kaida
Ken-ichi
K
Kusunoki
Susumu
S
Ueda
Masami
M
Kimura
Fumihiko
F
Kamakura
Keiko
K
D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000276:immunology*; D006801:Humans; D019846:Miller Fisher Syndrome / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D005732:Gangliosides; C084318:GT1aalpha ganglioside; D005677:G(M1) Ganglioside; C039129:GQ1b ganglioside; C018835:asialo GM1 ganglioside