A comparative proteomic analysis of rice seedlings under various high-temperature stresses.
To understand the responses of rice seedlings to different high-temperature stresses, seven-day-old rice seedlings were exposed to different high temperatures for 48 h, and the maximal quantum yield of PS II photochemistry measurements, ascorbate peroxidase activity assays and proteomic analyses in leaf tissue were performed. The results showed that when rice seedlings were exposed to high temperatures at 35 degrees C, 40 degrees C and 45 degrees C, the maximal quantum yield of photosystem II photochemistry, the activity of ascorbate peroxidase and the proteome changed greater at higher temperature. The proteomics analysis showed that proteins such as lignification-related proteins were regulated by high temperature and distinct proteins related to protection were up-regulated at different high temperatures. All the results indicated that different strategies were adopted at different levels of high temperature: the higher the temperature, the more protection machineries were involved. At 35 degrees C, some protective mechanisms were activated to maintain the photosynthetic capability. At 40 degrees C, antioxidative pathways were also active. When rice seedlings encountered high-temperature stress at 45 degrees C, in addition to those induced at 35 degrees C and 40 degrees C, heat shock proteins were effectively induced.
Han
Feng
F
Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, P.R. China.
Chen
Hui
H
Li
Xiao-Juan
XJ
Yang
Ming-Feng
MF
Liu
Gong-She
GS
Shen
Shi-Hua
SH
D060387:Ascorbate Peroxidases; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D012275:Oryza / Q000235:genetics*; D010544:Peroxidases / Q000235:genetics; D010788:Photosynthesis / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D036226:Seedlings / Q000235:genetics
D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012333:RNA, Messenger; D010544:Peroxidases; D060387:Ascorbate Peroxidases