Pyrrolidine dithiocarbamate attenuates paraquat-induced lung injury in rats.
Paraquat (PQ) has been demonstrated that the main target organ for the toxicity is the lung. This study aimed to investigate the potential protective effect of PDTC on the PQ-induced pulmonary damage. Fifty-four rats were divided into control, PQ-treated and PQ+PDTC-treated groups. Rats in the PQ group were administrated 40 mg/kg PQ by gastric gavage, and PDTC group with 40 mg/kg PQ followed by injection of 120 mg/kg PDTC (IP). On the days 3, 7, 14 and 21 after treatments, the activities of GSH-Px, SOD, MDA level and the content of HYP were measured. TGF-beta1 mRNA and protein were assayed by RT-PCR and ELISA. MDA level in plasma and BALF was increased and the activities of GSH-Px and SOD were decreased significantly in the PQ-treated groups (P < .05) compared with control group. While the activities of GSH-Px and SOD in the PQ+PDTC-treated groups was markedly higher than that of PQ-treated groups (P < .05), and in contrast, MDA level was lower. TGF-beta1 mRNA and protein were significantly lower in the PQ+PDTC-treated groups than that of PQ-treated groups (P < .05). The histopathological changes in the PQ+PDTC-treated groups were milder than those of PQ groups. Our results suggested that PDTC treatment significantly attenuated paraquat-induced pulmonary damage.
Chang
Xiuli
X
Department of Occupational Health and Toxicology, School of Public Health, Fudan University, Shanghai 200032, China.
Shao
Chunfeng
C
Wu
Qing
Q
Wu
Qiangen
Q
Huang
Min
M
Zhou
Zhijun
Z
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D016207:Cytokines / Q000032:analysis*; D004195:Disease Models, Animal; D006801:Humans; D055370:Lung Injury / Q000139:chemically induced* / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D010269:Paraquat; D011759:Pyrrolidines / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000032:analysis*; D013859:Thiocarbamates / Q000008:administration & dosage*; D016896:Treatment Outcome
D000975:Antioxidants; D016207:Cytokines; D011759:Pyrrolidines; D017382:Reactive Oxygen Species; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D010269:Paraquat