Role of airway epithelium in engulfing apoptotic eosinophils.
BACKGROUND
This study aims to investigate which recognition pathways are important in engulfing apoptotic eosinophils.


METHODS
Here, two epithelial cell were selected namely (large airway bronchial epithelial cells) LAECs and A549. The inhibition assay was examined by resting and dexamethasone-stimulated epithelial cells. Confocal microscopy confirmed the engulfment of apoptotic eosinophils.


RESULTS
Macrophages and LAECs recognized and phagocytosed apoptotic eosinophils. Dexamethasone and IL-1 increased the capacity of LAECs to engulf apoptotic eosinophils. More interestingly, inhibiting monoclonal antibodies (Mabs) abolished the uptake of apoptotic cells by LAECs.


CONCLUSIONS
On the basis of the above findings, the LAECs is capable of recognizing and engulfing apoptotic eosinophils and that enhanced by interleukin-1 (IL-1â) and dexamethasone.
Alenzi
Faris Q
FQ
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, AlKharaj 11942, Saudi Arabia. falenzi@ksu.edu.sa
D017209:Apoptosis / Q000502:physiology*; D001980:Bronchi / Q000166:cytology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000166:cytology / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D020287:In Situ Nick-End Labeling; D007375:Interleukin-1 / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000166:cytology / Q000502:physiology
D005938:Glucocorticoids; D007375:Interleukin-1; D003907:Dexamethasone