[Expression of VEGF and NGF in gliomas of human].
OBJECTIVE
To examine the expression of vascular endothelial cell growth factor (VEGF) and nerve growth factor (NGF) in gliomas of human, and investigate the relationship between VEGF, NGF and pathologic grading as well as tumorigenesis of gliomas.


METHODS
Immunohistochemistry (SP) and Western blot were applied to evaluate the expression of VEGF and NGF in 5 cases of normal controls and 20 cases of human gliomas.


RESULTS
VEGF and NGF were expressed in both normal brain tissues and gliomas. VEGF was observed mainly in the cytoplasm of gliomas, while NGF were occurred in both cytoplasm and nucleus of gliomas. Western blot analysis demonstrated that the expression level of VEGF and NGF was significantly higher than that in normal controls (P< 0.05), and a significant difference in vary pathologic grading gliomas was also observed.


CONCLUSIONS
VEGF and NGF were expressed in both the normal brain tissues and gliomas. The level of VEGF and NGF in gliomas was upregulated than that of the normal controls. The results indicate VEGF and NGF may play an important role in the tumorigenesis of gliomas, and relate to the extent of gliomas malignant.
Li
Jing-tao
JT
Department of Minimally Invasive Neurosurgery, No 1 Affiliated Hospital, Kunming Medical College, Kunming 650031, China.
Yan
Qi
Q
Yu
Hua-lin
HL
D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
C502240:NGF protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D020932:Nerve Growth Factor