title: Pattern of DAP12 expression in leukocytes from both healthy and systemic lupus erythematosus patients.
abstract: DAP12 is an ITAM-bearing transmembrane adaptor originally identified on the surface of Natural Killer cells. A broad expression among other immune cells was later found in myeloid and lymphoid cells. However, data on DAP12 expression pattern rely only on immunoblot and microarray analysis. Here, we describe the generation and the characterization of an anti-DAP12 monoclonal antibody. Using this novel reagent, we show that DAP12 expression is restricted to innate immune cells in basal condition. Since a decreased expression of DAP12 has been suggested in NK cells of systemic lupus erythematosus patients, we have further investigated the NK cell receptor repertoire and leukocyte expression of DAP12 in these patients and no major changes were detectable when compared to controls.
authors.affiliation: Department of Internal Medicine, CHU Conception, AP-HM, Marseille, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Schleinitz
authors.forename: Laurent
authors.initials: L
authors.lastname: Chiche
authors.forename: Sophie
authors.initials: S
authors.lastname: Guia
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Bouvier
authors.forename: Julie
authors.initials: J
authors.lastname: Vernier
authors.forename: Alexis
authors.initials: A
authors.lastname: Morice
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Houssaint
authors.forename: Jean Robert
authors.initials: JR
authors.lastname: Harlé
authors.forename: Gilles
authors.initials: G
authors.lastname: Kaplanski
authors.forename: Félix A
authors.initials: FA
authors.lastname: Montero-Julian
authors.forename: Frédéric
authors.initials: F
authors.lastname: Vély
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000097:blood*; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D011971:Receptors, Immunologic / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; D011971:Receptors, Immunologic; C110546:TYROBP protein, human