title: [Function of ferric reductase FRP1 gene in Candida albicans].
abstract: The ability of iron acquisition in Candida albicans has an effect on its growth and pathogenesis. Ferric reductases are important components of high affinity iron acquisition system in C. albicans.
  
  
  OBJECTIVE
  The aim of this study was to elucidate the function of FRP1 (Ferric reductase protein).
  
  
  METHODS
  FRP1 gene expression was detected under low-iron and high-iron conditions by Northern blot. We used the method of PCR-directed gene disruption to construct frp 1 null mutant and then the phenotypes of frp1delta/delta mutant were characterized.
  
  
  RESULTS
  Low-iron condition induced FRP1 gene expression. frp1delta/delta mutant showed no growth on low-iron media compared to wild-type strains on solid plates.
  
  
  CONCLUSIONS
  FRP1 protein is probably the main ferric reductase under low-iron condition.
authors.affiliation: Key Laboratory of Molecular and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China. hnsdly@yahoo.com.cn
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Wen
authors.initials: W
authors.lastname: Zheng
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wei
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
mesh_terms: D015152:Blotting, Northern; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics; D038181:FMN Reductase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D007501:Iron / Q000378:metabolism; D009154:Mutation
chemical_list: D005656:Fungal Proteins; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase