Improved thymic index, density and output in HIV-infected patients following low-dose growth hormone therapy: a placebo controlled study.
OBJECTIVE
To investigate the effect of low-dose, long-term recombinant human growth hormone (rhGH) therapy on immune reconstitution in human immunodeficiency virus (HIV)-infected patients with focus on thymic index, density and output.


METHODS
Randomized, placebo-controlled, double-blind, single-centre trial.


METHODS
Forty-six HIV-infected Caucasian men on highly active antiretroviral therapy, 21-60 years of age, were included. Twenty-eight patients were randomized to 0.7 mg/day rhGH and 18 patients to placebo, administrated as daily subcutaneous injections between 1300 and 1500 h for 40 weeks. Endpoints were changes from baseline in thymic size and thymic output measured as T-cell receptor rearrangement excision circles (TREC) frequency and total TREC content, and total and naive CD4 cells.


RESULTS
Thymic density and thymic index increased in the GH group, compared with the placebo group (28 versus 4 Hounsfield units, P = 0.006 and 1 versus 0, P = 0.004). TREC frequency and total TREC content increased in the GH group, compared with the placebo group (37 versus -8%, P = 0.049 and 51 versus -14%, P = 0.026). Total CD4 cells and naive CD4+ cells increased insignificantly more in the GH than the placebo group [11.4%, 95% confidence interval (CI) -6.0 to 28.9; P = 0.19 and 18%, interquartile range (IQR) -4, 40 versus 13%, IQR -12, 39; P = 0.79]. Therapy was well tolerated.


CONCLUSIONS
Daily treatment with a low dose rhGH of 0.7 mg for 40 weeks stimulated thymopoiesis expressed by thymic index, density and area, TREC frequency and total TREC content in CD4 cells in HIV-infected patients on highly active antiretroviral therapy.
Hansen
Birgitte R
BR
Clinical Research Centre, Copenhagen University Hospital, Hvidovre, Denmark. brhansen@dadlnet.dk
Kolte
Lilian
L
Haugaard
Steen B
SB
Dirksen
Carsten
C
Jensen
Frank K
FK
Ryder
Lars P
LP
Sørensen
Anna Louise
AL
Flyvbjerg
Allan
A
Nielsen
Susanne D
SD
Andersen
Ove
O
D000328:Adult; D015496:CD4-Positive T-Lymphocytes; D004311:Double-Blind Method; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology; D019382:Human Growth Hormone / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009929:Organ Size / Q000187:drug effects / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage; D013950:Thymus Gland / Q000033:anatomy & histology / Q000187:drug effects* / Q000276:immunology; D016896:Treatment Outcome; D055815:Young Adult
D011994:Recombinant Proteins; D019382:Human Growth Hormone