Processing of semen can result in increased sperm DNA fragmentation.
Processing of semen for assisted reproductive technologies (ART) entails a number of procedures that include semen liquefaction, removal of seminal plasma by centrifugation, incubation, and cryopreservation. The results of this study indicate that incubation of semen at room temperature and semen cryopreservation can result in increased levels of sperm DNA fragmentation.
Toro
Estefanía
E
Institut Marquès, Service of Reproduction, Barcelona, Spain.
Fernández
Silvia
S
Colomar
Ana
A
Casanovas
Aïda
A
Alvarez
Juan G
JG
López-Teijón
Marisa
M
Velilla
Esther
E
D000328:Adult; D002498:Centrifugation / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D008875:Middle Aged; D027724:Reproductive Techniques, Assisted; D012662:Semen Preservation / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013094:Spermatozoa / Q000473:pathology*; D013696:Temperature