Differential Shh, Bmp and Wnt gene expressions during craniofacial development in mice.
In this study, Bmp-4, Wnt-5a and Shh gene expressions were compared during early craniofacial development in mice by comparative non-isotopic in situ hybridization. Wild-type C57BL/6J mice were studied at various stages of embryonic development (from 8.5- to 13.5-day-old embryos--E8.5-13.5). During early odontogenesis, transcripts for Bmp-4, Shh and Wnt-5a were co-localised at the tooth initiation stage. At E8.5, Shh mRNA expression was restricted to diencephalon and pharyngeal endoderm. Before maxillae and mandible ossification, Bmp-4 and Wnt-5a signals were detected in the mesenchymal cells and around Meckel's cartilage. During palatogenesis, Shh was expressed only in the epithelium and Wnt-5a only in the mesenchyme of the elevating palatal shelves. During tongue development, Shh expression was found in mesenchyme, probably contributing to tongue miogenesis, while Wnt-5a signal was in the epithelium, possibly during placode development and papillae formation. Taken together, these findings suggest that Bmp-4, Shh and Wnt-5a gene expressions may act together on the epithelial-mesenchymal interactions occurring in several aspects of the early mouse craniofacial development, such as odontogenesis, neuronal development, maxillae and mandible ossification, palatogenesis and tongue formation.
Paiva
Katiucia Batista Silva
KB
Department of Oral Pathology, Dental School, University of São Paulo, São Paulo, Brazil. katipaiva@yahoo.com.br
Silva-Valenzuela
Maria das Graças
Md
Massironi
Silvia Maria Gomes
SM
Ko
Gui Mi
GM
Siqueira
Filipe Modolo
FM
Nunes
Fabio Daumas
FD
D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics*; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism*; D004707:Endoderm / Q000196:embryology / Q000378:metabolism; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D005145:Face / Q000196:embryology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D053823:Hedgehog Proteins / Q000235:genetics*; D007568:Jaw / Q000196:embryology / Q000378:metabolism; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009055:Mouth / Q000196:embryology / Q000378:metabolism; D009297:Nasal Mucosa / Q000378:metabolism; D009666:Nose / Q000196:embryology; D010159:Palate / Q000196:embryology / Q000378:metabolism; D012886:Skull / Q000196:embryology* / Q000378:metabolism; D014059:Tongue / Q000196:embryology / Q000378:metabolism; D014070:Tooth / Q000196:embryology / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics*; D000071818:Wnt-5a Protein
D055415:Bone Morphogenetic Protein 4; D053823:Hedgehog Proteins; C465570:Shh protein, mouse; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C438615:Wnt5a protein, mouse