title: [Effects of Simvastatin on osteoblast activity of human periodontal ligament cells].
abstract: OBJECTIVE
  To study the effects of Simvastatin on the osteoblast activity of human periodontal ligament (PDL) cells.
  
  
  METHODS
  The third passage human PDL were cultured in conditional mineralization medium with different concentrations of Simvastatin. The cells were divided into A group (0 mol/L), B group (1 x 10(-9) mol/L), C group (1 x 10(-8) mol/L), D group (1 x 10(-7) mol/L) and E group (1 x 10(-6) mol/L). Alkaline phosphatase (ALP) activity, osteopontin (OPN) and capability of mineralization were measured.
  
  
  RESULTS
  Differentiation osteoblast and mineralization of human PDL were improved in all treatment groups with different concentrations of Simvastatin (1 x 10(-9), 1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol/L). Compared with control group, statistically significant differences were found in 1 x 10(-8) mol/L, 1 x 10(-7) mol/L and 1 x 10(-6) mol/L groups (P<0.05). The maximum effect was observed at the concentration of 1 x 10(-7) mol/L.
  
  
  CONCLUSIONS
  Optimal concentration of Simvastatin can improve the osteoblastic activity of human PDL.
authors.affiliation: Dept. of Orthodontics, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.forename: Xue-Yang
authors.initials: XY
authors.lastname: Zhang
authors.forename: Chun-Xian
authors.initials: CX
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D010006:Osteoblasts; D010012:Osteogenesis; D053495:Osteopontin; D010513:Periodontal Ligament; D019821:Simvastatin
chemical_list: D053495:Osteopontin; D019821:Simvastatin; D000469:Alkaline Phosphatase