Enantioselective determination of trantinterol in rat plasma by ultra performance liquid chromatography-electrospray ionization mass spectrometry after derivatization.
An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination of trantinterol enantiomers in rat plasma. Diphenhydramine was employed as the internal standard. The plasma samples were prepared using liquid-liquid extraction with n-hexane-dichloromethane-isopropanol (20:10:1, v/v/v) as the extractant. Trantinterol enantiomers after pre-column derivatization using diacetyl-l-tartaric anhydride (DATAAN) were separated on a C18 column using a gradient solvent programme. The mobile phase was composed of 3mM ammonium acetate and acetonitrile. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI). Linear calibration curve for each enantiomer was obtained in the concentration range of 1-80 ng/mL, with limit of quantification (LOQ) of 1 ng/mL. The intra- and inter- precision (R.S.D.) values were below 9.6% and accuracy (R.E.) was from -2.4 to 6.2% at all quality control (QC) levels. The developed method was applied to the enantioselective pharmacokinetic study of trantinterol in rats.
Yang
Jing
J
Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang, PR China.
Wang
Yanjuan
Y
Pan
Li
L
Li
Ning
N
Lu
Xiumei
X
Guan
Jin
J
Cheng
Maosheng
M
Li
Famei
F
D000818:Animals; D002853:Chromatography, Liquid; D002976:Clenbuterol / Q000031:analogs & derivatives; D013058:Mass Spectrometry; D010949:Plasma; D051381:Rats
C542064:trantinterol; D002976:Clenbuterol