title: Impact of platelets and fresh frozen plasma in contrast to red cell concentrate on unstimulated and stimulated cytokine release in an in vitro model of transfusion.
abstract: Transfusion of blood may contribute to immunomodulation. Leuco-depleted standard blood products are supposed to result in less immunomodulation compared with whole blood. To determine the influence of leuco-depleted blood products on the cytokine response, red blood cell concentrates (RBC), fresh frozen plasma (FFP) and platelet concentrates (PC) were investigated in an in vitro model of blood transfusion. Leuco-depleted standard blood bank RBC, FFP and PC were mixed in vitro with AB0 compatible venous blood from healthy volunteers in ratios of 3:1, 1:1 and 1:3. Specimens were incubated in presence or absence of lipopolysaccharide, 1 mug/ml. After 24 h of incubation cytokine release of tumour necrosis factor (TNF)-alpha and interleukin-10 (IL-10) was measured in cell culture supernatants by means of enzyme-linked immunsorbent assay. Addition of RBC, FFP and PC to venous blood from healthy volunteers led to a significant and dose-dependent increase in spontaneous TNF-alpha and IL-10 release. After endotoxin stimulation, RBC, FFP and PC significantly suppressed the TNF-alpha response, while the stimulated release of IL-10 tended to increase, reaching significance only after high doses of FFP. Addition of leuco-depleted blood products changed the spontaneous and stimulated cytokine response in an in vitro model of transfusion. These data may suggest a possible contribution of transfused FFP and PC to immunomodulation after transfusion similar to RBC.
authors.affiliation: Department of Anaesthesiology, Critical Care Medicine and Pain Therapy, Saarland, Homburg, Germany.
authors.forename: S O
authors.initials: SO
authors.lastname: Schneider
authors.forename: H
authors.initials: H
authors.lastname: Rensing
authors.forename: S
authors.initials: S
authors.lastname: Gräber
authors.forename: S
authors.initials: S
authors.lastname: Kreuer
authors.forename: S
authors.initials: S
authors.lastname: Kleinschmidt
authors.forename: S
authors.initials: S
authors.lastname: Kreimeier
authors.forename: P
authors.initials: P
authors.lastname: Müller
authors.forename: A M
authors.initials: AM
authors.lastname: Mathes
authors.forename: A E
authors.initials: AE
authors.lastname: Biedler
mesh_terms: D016913:Blood Component Transfusion; D001792:Blood Platelets / Q000276:immunology* / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000819:agonists / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology; D008954:Models, Biological; D010949:Plasma / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10