[Basic Review Committee for Clinical Laboratory Standardization. Column 1. Activity report of 2007. Group items of authentic sample, Working Group J2].
(1) Concerning measurement of serum levels of insulin, C-peptide, and cortisol, a joint experiment in 2006 suggested that a reference material derived from authentic samples was effective for the convergence of data. (2) To reconfirm the convergence of data using a candidate reference material derived from authentic sample, we generated pooled serum samples of patients as candidate materials, and the levels of insulin and C-peptide in the sera of healthy subjects and the level of cortisol in panel serum IRMM ERM-DA45I-1-DA45I-3 were measured. (3) The serum levels measured with commercially available reagents were reconfirmed to lead to data convergence. (4) We employed reagents which were traceable in reference materials of authentic samples, calibrated each reagent, and confirmed the convergence of data, as verified in the previous examination. (5) The prototype reference material of authentic sample was proven to be effective as a standard reference material.
Mochizuki
Katsuhiko
K
Manager Marketing Planning Group, Chuo-ku, Tokyo 103-0007, Japan. kk-mochizuki@fujirebio.co.jp
D026683:Advisory Committees; D002096:C-Peptide / Q000097:blood / Q000592:standards; D019411:Clinical Laboratory Techniques / Q000592:standards*; D004355:Drug Stability; D006854:Hydrocortisone / Q000097:blood / Q000592:standards; D007328:Insulin / Q000097:blood / Q000592:standards; D010338:Pathology, Clinical / Q000458:organization & administration / Q000592:standards*
D002096:C-Peptide; D007328:Insulin; D006854:Hydrocortisone