title: Activities of the enzymes that hydrolyze adenine nucleotides in platelets from multiple sclerosis patients.
abstract: Multiple sclerosis (MS) is the most common chronic disabling neurological disease in young adults. Alterations in platelet function have been observed in MS; however, the mechanism and the relevance of this blood cell disorder with regard to MS pathogenesis are not yet understood. The aim of this study was to evaluate activities of ectonucleoside thiphosphate diphosphohydrolase (NTPDase, CD39), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), 5'-nucleotidase and adenosine deaminase (ADA) in platelets from patients with the relapsing-remitting form of MS (RRMS), as well as to analyze platelet aggregation and expression of NTPDase. The results obtained show that NTPDase, 5'-nucleotidase, E-NPP and ADA activities were decreased in platelets of RRMS patients when compared with the control group (p < 0.05). In addition, NTPDase expression in platelets was also decreased in these patients (p < 0.05); however, no differences were observed in platelet aggregation between RRMS patients and the control group. Our results suggest that the alterations in NTPDase, E-NPP, 5'-nucleotidase and ADA may have contributed to the alterations in platelet function in MS by altering the levels of nucleotides and nucleosides in the circulation.
authors.affiliation: Department of Biochemistry, Institute of Basic Sciences for Health, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Roselia Maria
authors.initials: RM
authors.lastname: Spanevello
authors.forename: Cinthia Melazzo
authors.initials: CM
authors.lastname: Mazzanti
authors.forename: Margarete
authors.initials: M
authors.lastname: Bagatini
authors.forename: Maisa
authors.initials: M
authors.lastname: Correa
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.forename: Gustavo
authors.initials: G
authors.lastname: Thomé
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Lara
authors.initials: L
authors.lastname: Becker
authors.forename: Luziane
authors.initials: L
authors.lastname: Bellé
authors.forename: Liliane
authors.initials: L
authors.lastname: de Oliveira
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D001081:Apyrase / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology* / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006868:Hydrolysis; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000201:enzymology* / Q000503:physiopathology; D009708:Nucleotidases / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010974:Platelet Aggregation
chemical_list: D000227:Adenine Nucleotides; D015703:Antigens, CD; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009708:Nucleotidases; D010727:Phosphoric Diester Hydrolases; D000243:Adenosine Deaminase; D001081:Apyrase; C067869:CD39 antigen