title: The twin spot generator for differential Drosophila lineage analysis.
abstract: In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.
authors.affiliation: Commissariat à l'Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France. rgriffin@receptor.med.harvard.edu
authors.forename: Ruth
authors.initials: R
authors.lastname: Griffin
authors.forename: Anne
authors.initials: A
authors.lastname: Sustar
authors.forename: Marianne
authors.initials: M
authors.lastname: Bonvin
authors.forename: Richard
authors.initials: R
authors.lastname: Binari
authors.forename: Alberto
authors.initials: A
authors.lastname: del Valle Rodriguez
authors.forename: Amber M
authors.initials: AM
authors.lastname: Hohl
authors.forename: Jack R
authors.initials: JR
authors.lastname: Bateman
authors.forename: Christians
authors.initials: C
authors.lastname: Villalta
authors.forename: Elleard
authors.initials: E
authors.lastname: Heffern
authors.forename: Didier
authors.initials: D
authors.lastname: Grunwald
authors.forename: Chris
authors.initials: C
authors.lastname: Bakal
authors.forename: Claude
authors.initials: C
authors.lastname: Desplan
authors.forename: Gerold
authors.initials: G
authors.lastname: Schubiger
authors.forename: C-ting
authors.initials: CT
authors.lastname: Wu
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002999:Clone Cells; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D005470:Fluorometry; D023281:Genomics / Q000295:instrumentation / Q000379:methods*; D008938:Mitosis; D009154:Mutation; D011995:Recombination, Genetic