title: Two chromatin remodeling activities cooperate during activation of hormone responsive promoters.
abstract: Steroid hormones regulate gene expression by interaction of their receptors with hormone responsive elements (HREs) and recruitment of kinases, chromatin remodeling complexes, and coregulators to their target promoters. Here we show that in breast cancer cells the BAF, but not the closely related PBAF complex, is required for progesterone induction of several target genes including MMTV, where it catalyzes localized displacement of histones H2A and H2B and subsequent NF1 binding. PCAF is also needed for induction of progesterone target genes and acetylates histone H3 at K14, an epigenetic mark that interacts with the BAF subunits by anchoring the complex to chromatin. In the absence of PCAF, full loading of target promoters with hormone receptors and BAF is precluded, and induction is compromised. Thus, activation of hormone-responsive promoters requires cooperation of at least two chromatin remodeling activities, BAF and PCAF.
authors.affiliation: Centre de Regulació Genòmica (CRG), Universitat Pompeu Fabra, Parc de Recerca Biomèdica (PRBB), Barcelona, Spain.
authors.forename: Guillermo Pablo
authors.initials: GP
authors.lastname: Vicent
authors.forename: Roser
authors.initials: R
authors.lastname: Zaurin
authors.forename: A Silvina
authors.initials: AS
authors.lastname: Nacht
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Jofre
authors.initials: J
authors.lastname: Font-Mateu
authors.forename: Francois
authors.initials: F
authors.lastname: Le Dily
authors.forename: Michiel
authors.initials: M
authors.lastname: Vermeulen
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
authors.forename: Miguel
authors.initials: M
authors.lastname: Beato
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005260:Female; D006728:Hormones / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D006728:Hormones; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C504738:SMARCE1 protein, human