Rudaea cellulosilytica gen. nov., sp. nov., isolated from soil.
A yellow-pigmented, Gram-negative, aerobic, rod-shaped bacterium, strain KIS3-4T, was isolated from soil collected on Daechung Island in the West Sea of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain KIS3-4T in a distinct lineage in the family Xanthomonadaceae. Strain KIS3-4T shared 87.3-93.7% sequence similarity with members of the family Xanthomonadaceae, and was related most closely to the genera Dyella and Dokdonella. In its biochemical characteristics, strain KIS3-4T was clearly separable from other genera within the family Xanthomonadaceae on the basis of the hydrolysis of cellulose and urea, high G+C content (64 mol%) and fatty acid profile. Major fatty acids (>10% of the total fatty acids) were iso-C17:1omega9c (32.8%), iso-C17:0 (18.0%) and iso-C16:0 (12.7%). Q-8 was the predominant respiratory quinone. Phosphatidylethanolamine and several unidentified aminophospholipids and phospholipids were present. Based on its unique phenotypic, genotypic and phylogenetic features, strain KIS3-4T represents a novel genus and species, for which the name Rudaea cellulosilytica gen. nov., sp. nov. is proposed. The type strain of Rudaea cellulosilytica is KIS3-4T (=KACC 12734T=JCM 15422T).
Weon
Hang-Yeon
HY
Mushroom Research Division, National Institute of Horticultural and Medicinal Crop, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Yoo
Seung-Hee
SH
Kim
Yoo-Jeong
YJ
Lee
Chang-Muk
CM
Kim
Byung-Yong
BY
Jeon
Young-Ah
YA
Hong
Seung-Beom
SB
Anandham
Rangasamy
R
Kwon
Soon-Wo
SW
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002482:Cellulose / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011309:Preservation, Biological; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014508:Urea / Q000378:metabolism; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D014508:Urea; D002482:Cellulose