Strategy for extracting DNA from clay soil and detecting a specific target sequence via selective enrichment and real-time (quantitative) PCR amplification.
We present a simple strategy for isolating and accurately enumerating target DNA from high-clay-content soils: desorption with buffers, an optional magnetic capture hybridization step, and quantitation via real-time PCR. With the developed technique, microg quantities of DNA were extracted from mg samples of pure kaolinite and a field clay soil.
Yankson
Kweku K
KK
Department of Biology, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Steck
Todd R
TR
D000538:Aluminum Silicates / Q000737:chemistry*; D001431:Bacteriological Techniques; D000077215:Clay; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology
D000538:Aluminum Silicates; D004247:DNA; D000077215:Clay