Fern plant-derived protoapigenone leads to DNA damage, apoptosis, and G(2)/m arrest in lung cancer cell line H1299.
Protoapigenone, isolated from the native fern plant Thelypteris torresiana, has anticancer activity against some cancer cells. However, the toxicological mechanism for protoapigenone is still unknown. Here, we investigated the anticancer effect of protoapigenone on human lung cancer cell lines. The comet assay showed that DNA damage induced by protoapigenone is dose-dependent. Trypan blue exclusion showed that the cell killing by protoapigenone is both time and dose dependent. The IC(50) of protoapigenone for 12, 24, and 48 h in H1299 cells is 6.11, 2.74, and 1.49 microM, respectively. Flow cytometry showed cell cycle perturbation such as sub-G(1) accumulation (at 1.57 microM for 48 h and at 3.57 microM for 12 and 24 h) and G(2)/M arrest (at 3.57 microM for 12 and 24 h) for protoapigenone. The sub-G(1) accumulation phenomena in the 3.57 microM for 24 h sample were shown to be apoptosis using Annexin V-immunofluorescence/propidium iodide staining. These results suggest protoapigenone is a potential chemotherapeutic agent for lung cancers.
Chiu
Chien-Chih
CC
Department of Biotechnology, Kaohsiung Medical University , Kaohsiung, Taiwan .
Chang
Hsueh-Wei
HW
Chuang
Da-Wei
DW
Chang
Fang-Rong
FR
Chang
Yu-Ching
YC
Cheng
Yu-Shan
YS
Tsai
Ming-Tz
MT
Chen
Wan-Yu
WY
Lee
Su-Shuo
SS
Wang
Chih-Kuang
CK
Chen
Jeff Yi-Fu
JY
Wang
Hui-Min
HM
Chen
Chao-Chieh
CC
Liu
Yin-Chang
YC
Wu
Yang-Chang
YC
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D020552:Comet Assay; D003512:Cyclohexanones / Q000494:pharmacology*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D029624:Ferns / Q000737:chemistry*; D047309:Flavones / Q000494:pharmacology*; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*
D000972:Antineoplastic Agents, Phytogenic; D003512:Cyclohexanones; D047309:Flavones; C524112:protoapigenone