title: [Effect of basic fibroblast growth factor on the gene expression of epidermal growth factor receptor by periodontal ligament cells in culture].
abstract: OBJECTIVE
  To study the effect of basic fibroblast growth factor (bFGF) on the gene expression of epidermal growth factor receptor (EGFR) by periodontal ligament cells (PDLC) in culture and discuss the effect of bFGF in cell differentiation and periodontal regeneration.
  
  
  METHODS
  Human PDLC were cultured in vitro and cell clone was obtained by the method of limiting dilution. PDLC were stimulated by bFGF, and then gene expression of EGFR was assessed by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The mRNA expression of EGFR was promoted by bFGF and the effect was dose-dependent.
  
  
  CONCLUSIONS
  The promotion effect of the mRNA expression of EGFR in PDLC may be one of important factors which participate in the healing process of periodontitis and provide partly theoretical basis of cell differentiation and periodontal regeneration.
authors.affiliation: Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Chong-Tao
authors.initials: CT
authors.lastname: Lin
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D066246:ErbB Receptors; D016222:Fibroblast Growth Factor 2; D006801:Humans; D010513:Periodontal Ligament; D012333:RNA, Messenger; D012038:Regeneration
chemical_list: D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D066246:ErbB Receptors