2-D DIGE analysis of the proteome of extracts from peanut variants reveals striking differences in major allergen contents.
Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food allergens causing severe anaphylactic reactions, pre-packaged food containing peanut components needs to be classified according to European ruling since 2005. Food companies search for strategies to reduce the allergenicity of peanut-derived food additives either by genetically altering the allergen content or by identifying peanut varieties with low levels of major allergens. In our study, we focused on peanut extracts from Indonesia that apparently contain lower levels of the major Arachis hypogaea allergen 1 (Ara h 1). Basic extracts of Virginia-type and Indonesian peanuts were compared by 1- and 2-DE. We identified more than hundred individual components in these extracts by MS and provide a high-resolution allergen map that also includes so far unknown fragments of major peanut allergens. The reduced level of Ara h 1 associated with a significantly lower abundance of the most potent peanut allergen Ara h 2 in various Indonesian peanuts was also confirmed by Western blotting with monoclonal antibodies and sera of allergic patients.
Schmidt
Hendrik
H
Laboratory for Molecular Immunology, Institute for Immunology, University Hospital Schleswig-Holstein Campus Kiel, Kiel, Germany.
Gelhaus
Christoph
C
Latendorf
Ties
T
Nebendahl
Melanie
M
Petersen
Arnd
A
Krause
Susanne
S
Leippe
Matthias
M
Becker
Wolf-Meinhard
WM
Janssen
Ottmar
O
D000911:Antibodies, Monoclonal / Q000276:immunology; D052179:Antigens, Plant / Q000032:analysis* / Q000276:immunology; D010367:Arachis / Q000737:chemistry*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry* / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D006023:Glycoproteins; D010936:Plant Extracts; D010940:Plant Proteins; D020543:Proteome