Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato.
The genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/ helicase domain in its C-terminal half, the p2 protein contained a RNA polymerase domain; the 3a protein contained a RNA-binding domain with α-helix and β-sheet secondary structures. In addition, stem-loop structures with potential capacity of protein interactions were predicted on the untranslated terminal regions. Comparison with the other sequenced PMoV isolate showed nucleotide identities of 93, 90, and 93% for genomic RNAs 1, 2 and 3, respectively, and amino acid identities ranging from 88 to 97% for the different proteins. A cytosine deletion was detected at position 1,366 of RNA 3, involving a start codon for the coat protein (CP) gene different from the other PMoV isolate, resulting in a CP 16 amino acids shorter. Comparison of synonymous and nonsynonymous mutations revealed different selective constraints along the genome.
Galipienso
Luis
L
Department of Plant Pathology, Institute of Agro-Alimentary and Technology Research (IRTA), 08348 Cabrils, Spain. luis.galipienso@irta.es
Rubio
Luis
L
López
Luis
L
Soler
Salvador
S
Aramburu
José
J
D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral; D019179:Ilarvirus / Q000235:genetics* / Q000302:isolation & purification*; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013030:Spain; D020506:Untranslated Regions; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D020506:Untranslated Regions; D014764:Viral Proteins