title: Dual targeting of a thermosensitive nanogel conjugated with transferrin and RGD-containing peptide for effective cell uptake and drug release.
abstract: In this paper, both arginine-glycine-aspartic acid (RGD)-containing peptide and transferrin (Tf) were conjugated to the thermosensitive poly(N-isopropylacrylamide-co-propyl acrylic acid) (poly(NIPAAm-co-PAAc)) nanogel to prepare a dual-targeting drug carrier. The obtained nanogel was characterized in terms of fluorescence spectroscopy, UV-vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). In order to track the dual-ligand conjugated nanogel, fluorescein isothiocyanate (FITC) was further conjugated to the nanogel. A cell internalization experiment showed that the dual-ligand conjugated nanogel exhibited obviously enhanced endocytosis by HeLa cells as compared with non-tumorous cells (COS-7 cells). The drug-loaded dual-ligand conjugated nanogel could be transported efficiently into the target tumor cells and the anti-tumor effect was enhanced significantly, suggesting that the dual-ligand conjugated nanogel has great potential as a tumor targeting drug carrier.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Chang-Yun
authors.initials: CY
authors.lastname: Quan
authors.forename: Cong
authors.initials: C
authors.lastname: Chang
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Chang-Sheng
authors.initials: CS
authors.lastname: Chen
authors.forename: Xiao-Ding
authors.initials: XD
authors.lastname: Xu
authors.forename: Si-Xue
authors.initials: SX
authors.lastname: Cheng
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
mesh_terms: D000178:Acrylamides; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002021:Buffers; D019556:COS Cells; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004317:Doxorubicin / Q000494:pharmacology*; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D000080385:Nanogels; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014168:Transferrin / Q000378:metabolism*
chemical_list: D000178:Acrylamides; D000970:Antineoplastic Agents; D002021:Buffers; D000080385:Nanogels; D009842:Oligopeptides; D010455:Peptides; D012996:Solutions; D014168:Transferrin; C512582:poly(N-isopropylacrylamide-co-propylacrylic acid); C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D004317:Doxorubicin; D011094:Polyethyleneimine; D016650:Fluorescein-5-isothiocyanate