title: Impact of different patterns of sperm chromosomal abnormalities on the chromosomal constitution of preimplantation embryos.
abstract: OBJECTIVE
  To evaluate the effect of sperm chromosome abnormalities--disomy for sex chromosomes and diploidy--in the chromosomal constitution of preimplantation embryos.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  Infertility clinic.
  
  
  METHODS
  Three groups: 46,XY infertile men with increased incidence of sex chromosome disomy in sperm; 46,XY infertile men with increased diploidy rates in sperm; 47,XYY infertile men with increased sex chromosome disomy and diploidy rates in sperm.
  
  
  METHODS
  Sperm collection for fluorescence in situ hybridization analysis. Embryo biopsy for preimplantation genetic screening.
  
  
  METHODS
  Frequencies of numerical abnormalities in sperm for chromosomes 13, 18, 21, X, and Y, and in embryos for chromosomes 13, 16, 18, 21, 22, X, and Y.
  
  
  RESULTS
  A significant increase of chromosomally abnormal and mosaic embryos was observed in the three study groups compared with controls. Those sperm samples with increased sex chromosome disomy rates produced significantly higher percentages of aneuploid embryos, with a threefold increase for sex chromosomes. Sperm samples with increased diploidy rates were mainly associated to the production of triploid embryos.
  
  
  CONCLUSIONS
  A strong correlation between sperm and embryo chromosomal constitution has been shown in infertile men with 46,XY and 47,XYY karyotypes.
authors.affiliation: Preimplantation Genetic Diagnosis Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain. Electronic address: lrodrigo@ivi.es.
authors.forename: Lorena
authors.initials: L
authors.lastname: Rodrigo
authors.affiliation: Preimplantation Genetic Diagnosis Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Peinado
authors.affiliation: Preimplantation Genetic Diagnosis Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain.
authors.forename: Emilia
authors.initials: E
authors.lastname: Mateu
authors.affiliation: Medical Reproduction Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Remohí
authors.affiliation: Medical Reproduction Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
authors.affiliation: Medical Reproduction Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.affiliation: Andrology Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gil-Salom
authors.affiliation: Preimplantation Genetic Diagnosis Unit, Instituto Valenciano de Infertilitad, IVI-Valencia, Valencia, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rubio
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000378:metabolism* / Q000473:pathology; D002869:Chromosome Aberrations / Q000196:embryology; D004171:Diploidy; D005260:Female; D006060:Gonadal Dysgenesis, Mixed / Q000235:genetics / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics / Q000473:pathology*; D008297:Male; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D012189:Retrospective Studies; D055101:Semen Analysis / Q000379:methods; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology*; D024182:Uniparental Disomy / Q000235:genetics / Q000473:pathology