title: Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes.
abstract: We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries.
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors: Oriel M M
authors: OM
authors: Thekisoe
authors: Natasha E
authors: NE
authors: Rambritch
authors: Ryo
authors: R
authors: Nakao
authors: Raoul S
authors: RS
authors: Bazie
authors: Peter
authors: P
authors: Mbati
authors: Boniface
authors: B
authors: Namangala
authors: Imna
authors: I
authors: Malele
authors: Robert A
authors: RA
authors: Skilton
authors: Frans
authors: F
authors: Jongejan
authors: Chihiro
authors: C
authors: Sugimoto
authors: Shin-Ichiro
authors: S
authors: Kawazu
authors: Noboru
authors: N
authors: Inoue
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002020:Buffaloes; D002063:Burundi; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D007630:Kenya; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics*; D012432:Rwanda; D012680:Sensitivity and Specificity; D013019:South Africa; D013636:Tanzania; D016797:Theileria parva / Q000235:genetics / Q000302:isolation & purification*; D013801:Theileriasis / Q000175:diagnosis* / Q000469:parasitology; D013997:Time Factors; D014454:Uganda
chemical_list: D000953:Antigens, Protozoan; D017931:DNA Primers; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C095023:antigen PIM, Theileria parva