title: Signal peptide of Arabinosidase enhances secretion of interferon-alpha2b protein by Bifidobacteria longum.
abstract: Bifidobacteria can potentially be used for gene therapy. Here, we reported that 65% of the total hIFN-alpha2b produced from Bifidobacteria longum transformed with pBAD-SPIFN plasmids encoding a fusion protein of the arabinosidase signal peptide and human IFN-alpha2b (hIFN-alpha2b), was secreted. For B. longum transformed with pBAD-IFN plasmids (hIFN-alpha2b without the signal peptide), only 15% of the total IFN-alpha2b was secreted and western blotting and N-terminal amino-acid sequence analysis revealed cleavage of the arabinosidase signal peptide from the secreted hIFN-alpha2b. Moreover, the active level of the secreted hIFN-alpha2b in the supernatant of B. longum transformed with pBAD-SPIFN plasmids was over 1,000 IU/ml commercial rhIFN-alpha2b. Hence, the arabinosidase signal peptide can enhance the secretion efficiency of IFN-alpha2b from B. longum.
authors: Department of Infectious Diseases, The Affiliated Shenzhen Nanshan Hospital of Guangdong Medical College, Nanshan District, 518052 Shenzhen, China. qiwendeng@hotmail.com
authors: Qiwen
authors: Q
authors: Deng
authors: Weiseng
authors: W
authors: Zeng
authors: Zhijian
authors: Z
authors: Yu
mesh_terms: D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D006026:Glycoside Hydrolases / Q000235:genetics*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D021382:Protein Sorting Signals / Q000235:genetics*; D021381:Protein Transport; D011994:Recombinant Proteins; D014169:Transformation, Bacterial
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase