Production of glyceric acid by Gluconobacter sp. NBRC3259 using raw glycerol.
Gluconobacter sp. NBRC3259 converted glycerol to glyceric acid (GA). The enantiomeric composition of the GA produced was a mixture of DL-forms with a 77% enantiomeric excess of D-GA. After culture conditions, such as initial glycerol concentration, types and amounts of nitrogen sources, and initial pH, were optimized, Gluconobacter sp. NBRC3259 produced 54.7 g/l of GA as well as 33.7 g/l of dihydroxyacetone (DHA) from 167 g/l of glycerol during 4 d of incubation in a jar fermentor with pH control. GA production from raw glycerol samples, the main by-product of the transesterification process in the biodiesel production and oleochemical industries, was also evaluated after proper pretreatment of the samples. Using a raw glycerol sample with activated charcoal pretreatment, 45.9 g/l of GA and 28.2 g/l of DHA were produced from 174 g/l of glycerol.
Habe
Hiroshi
H
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. hiroshi.habe@aist.go.jp
Shimada
Yuko
Y
Fukuoka
Tokuma
T
Kitamoto
Dai
D
Itagaki
Masayuki
M
Watanabe
Kunihiro
K
Yanagishita
Hiroshi
H
Sakaki
Keiji
K
D003470:Culture Media / Q000494:pharmacology; D004098:Dihydroxyacetone / Q000378:metabolism; D020576:Gluconobacter / Q000187:drug effects / Q000378:metabolism*; D005988:Glyceric Acids / Q000737:chemistry / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics
D003470:Culture Media; D005988:Glyceric Acids; C042971:glyceric acid; D004098:Dihydroxyacetone; D005990:Glycerol