Production of flavonoid o-glucoside using sucrose synthase and flavonoid o-glucosyltransferase fusion protein.
Enzymatic glucosylation with glycosyltransferases can be used to regulate the water solubility of aglycone. The drawback of this process is the demand of UDP-glucose as a sugar donor. We made an in-frame fusion of the flavonoid O-glucosyltransferase (OsUGT-3) and sucrose synthase (AtSUS) genes. The resulting fusion protein, OsUGT3-AtSUS, was expressed in E. coli and purified. When sucrose and UDP were supplied, SUS-UGT was able to convert quercetin into quercetin O-glucoside without the addition of UDP-glucose. In addition, UDPglucose was recycled when sucrose was added to the reaction mixture. This fusion protein is useful for the enzymatic production of flavonoid O-glucosides.
Son
Mi Hye
MH
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea.
Kim
Bong-Gyu
BG
Kim
Dae Hwan
DH
Jin
Mirim
M
Kim
Kwangpyo
K
Ahn
Joong-Hoon
JH
D004926:Escherichia coli / Q000378:metabolism; D005419:Flavonoids / Q000096:biosynthesis*; D005798:Genes, Bacterial; D005960:Glucosides; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D011794:Quercetin / Q000378:metabolism / Q000031:analogs & derivatives; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D013395:Sucrose / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D005419:Flavonoids; D005960:Glucosides; D011993:Recombinant Fusion Proteins; C532085:quercetin 3'-O-glucoside; D013395:Sucrose; D011794:Quercetin; D005964:Glucosyltransferases; C022699:sucrose synthase; D014532:Uridine Diphosphate Glucose