title: [Tissue microarray (TMA) validated progression markers in colorectal cancer using antibody microarrays].
abstract: BACKGROUND
  The exact molecular biological background of colorectal cancer development and progression are not hitherto known. Using microarray systems, hundreds or thousands of parameters could be examined simultaneously for answering the mentioned questions.
  
  
  OBJECTIVE
  To identify possible protein markers of colorectal cancer development and progression using antibody array, and the validation of these markers on tissue microarrays done with colorectal cancer samples. Furthermore, to determine colorectal cancer diagnostic marker combination in protein level.
  
  
  METHODS
  Surgically resected samples from ten Dukes B and six Dukes D stage patients containing both diseased and un-involved parts of the colon were freshly frozen. The samples were homogenized and the extracted proteins were used for Clontech AB500 arrays. Twelve selected genes were validated on tissue microarrays.
  
  
  RESULTS
  The expression of 67 proteins was altered (p < 0.05) between the normal colon and cancerous samples. These genes are related to apoptosis (n = 5), cell cycle regulation (n = 7), transcription (n = 4), DNA replication (n = 6) and other cell functions, such as transport and cell adhesion (n = 45). Twelve potential markers were immunohistochemically validated on morphological level by using tissue microarrays (CYCA1, HSP60, TOP1, APC, CBP, ERK, EGFR, C-myc, Cald, DARPP32, MRE11A, AR, EPS8).
  
  
  CONCLUSIONS
  Based on these results, validated colorectal cancer development related protein markers are involved in a wide spectrum of cell functions such as apoptosis, cell cycle regulation, and signal transduction. A set of six proteins has been determined, which helps to differentiate between normal specimen, early and late stage colorectal cancer with high sensitivity and specificity.
authors: Semmelweis Egyetem, Altalános Orvostudományi Kar, II. Belgyógyászati Klinika, Budapest. spisak@abc.hu
authors: Sándor
authors: S
authors: Spisák
authors: Barnabás
authors: B
authors: Galamb
authors: Barnabás
authors: B
authors: Wichmann
authors: Ferenc
authors: F
authors: Sipos
authors: Orsolya
authors: O
authors: Galamb
authors: Norbert
authors: N
authors: Solymosi
authors: Balázs
authors: B
authors: Nemes
authors: Zsolt
authors: Z
authors: Tulassay
authors: Béla
authors: B
authors: Molnár
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology* / Q000601:surgery; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology* / Q000601:surgery; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor