title: Profiling the autoantibody repertoire by screening phage-displayed human cDNA libraries.
abstract: The advent of the serological identification of antigens by procedures such as cDNA cloning and recombinant protein expression has allowed the direct molecular definition of immunogenic proteins. The phage-display technology provides several advantages over conventional immunoscreening procedures based on plasmid or lambda-phage cDNA libraries. So far, attempts to display open reading frames, such as those encoded by cDNA fragments, on filamentous phages have not been very successful. We managed to develop a strategy based on "folding reporters" which allows filtering out open reading frames from DNA and displaying them on filamentous phages in such a way that they are amenable to subsequent selection or screening. Once the cDNA library of interest is created, phage-display technology is used for selection of novel putative antigens; these are then validated by printing isolated protein on microarray and screening with patients' sera.
authors: Department of Life Sciences, University of Trieste, Trieste, Italy.
authors: Roberto
authors: R
authors: Di Niro
authors: Sara
authors: S
authors: D'Angelo
authors: Paola
authors: P
authors: Secco
authors: Roberto
authors: R
authors: Marzari
authors: Claudio
authors: C
authors: Santoro
authors: Daniele
authors: D
authors: Sblattero
mesh_terms: D001323:Autoantibodies / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008954:Models, Biological; D016366:Open Reading Frames; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology
chemical_list: D001323:Autoantibodies; D019151:Peptide Library; D011994:Recombinant Proteins