Wide-field extended-resolution fluorescence microscopy with standing surface-plasmon-resonance waves.
The resolution of conventional surface-plasmon-resonance (SPR) imaging has been limited by the diffraction nature of light. A wide-field extended-resolution optical imaging technique, standing-wave SPR fluorescence (SW-SPRF) microscopy, has been developed. Based on evanescent SPR standing waves, SW-SPRF provides lateral resolution approaching 100 nm and offers the advantages of significant signal enhancement and background-noise reduction. SW-SPRF has the potential for sensitive biomolecular detection, nanoscale imaging, and lithographic applications.
Chung
Euiheon
E
Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Kim
Yang-Hyo
YH
Tang
Wai Teng
WT
Sheppard
Colin J R
CJ
So
Peter T C
PT
D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*