High resistance to lipid peroxidation of bird heart mitochondria and microsomes: Effects of mass and maximum lifespan.
The aim of this investigation was to study the connection between body size, fatty acid composition and sensitivity to lipid peroxidation of heart mitochondria and microsomes isolated from different size bird species: manon (Lonchura striata), quail (Coturnix coturnix var japonica), pigeon (Columba livia), duck (Cairina moschata) and goose (Anser anser), representing a 372-fold range of body mass. Fatty acids of total lipids were determined using gas chromatography and lipid peroxidation was evaluated with a chemiluminescence assay. The fatty acids present in heart organelles of the different bird species analyzed showed a small number of significant allometric trends. In mitochondria, from the individual fatty acid data, palmitoleic acid (C16:1 n7) increased allometrically (r=0.878), while stearic acid (C18:0) was negatively related to body mass (r=-0.903). Interestingly, none of the calculated fatty acid variables, the average fatty acid saturated, monounsaturated, polyunsaturated (PUFA) and the unsaturation index (UI) was established to show significant body size-related variations. In heart microsomes, the content of C18:0 was significantly smaller (r=-0.970) in the birds of greater size. A significant allometric increase in linoleic acid (C18:2 n6) (r=0.986), polyunsaturated (r=0.990) and UI (r=0.904) was observed in the larger birds. The total n6 fatty acids of heart mitochondria did not show significant differences when it was correlated to body mass of the birds. Moreover, positive allometric relationships were shown for microsomes. The total n3 fatty acids of heart mitochondria and microsomes indicated no significant correlations to body mass of birds. The C16:1 n7, C18:0 in mitochondria and C18:0, C18:2 n6, PUFA, UI and PUFA n6 in microsomes showed significant differences when they were correlated to maximum life span (MLSP) of birds. As light emission=chemiluminescence originated from heart organelles was not statistically significant, a lack of correlation between the sensitivity to lipid peroxidation and body size or maximum life span was obtained. These results indicate that the high resistance of bird hearts to the attack by free radicals is body size-independent and would be related to the preservation of cardiac function.
Gutiérrez
A M
AM
Cátedra de Fisiología Animal, Facultad de Ciencias Naturales y Museo, Argentina.
Reboredo
G R
GR
Mosca
S M
SM
Catalá
A
A
D000818:Animals; D001717:Birds / Q000502:physiology*; D049628:Body Size; D010856:Columbidae / Q000502:physiology; D003370:Coturnix / Q000502:physiology; D004372:Ducks / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005777:Geese / Q000502:physiology; D006321:Heart / Q000033:anatomy & histology; D015227:Lipid Peroxidation; D008136:Longevity; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism
D005227:Fatty Acids