Scorpion venom peptides accelerate hematopoietic recovery of myelosuppression in irradiated mice.
Sublethally irradiated mice were administered with scorpion venom peptides (SVP) or with PBS in the saline control group, 3 days before and 7 consecutive days after irradiation. Hematopoietic recovery was assessed by bone marrow (BM) cell proliferation index (PI) and colony forming unit-granulocyte/macrophage (CFU-GM), spleen weight index (SI) and thymus weight index (TI), colony-forming unit-spleen (CFU-S) and peripheral leukocyte counts. In addition, IL-1alpha and SCF levels in BM, IL-6 and GM-CSF levels in serum were determined. In SVP treated groups, PI was improved dramatically versus control mice on day 22 after irradiation. The number of CFU-GM colonies in all SVP treated groups was higher than the control groups. The difference of the number of CFU-GM colonies between SVPV group (0.2 mg/kg) and the control was significant on day 5 and 10 after irradiation (p < 0.05). SVPIV (0.2 mg/kg) could activate the CFU-S formation on day 10 after irradiation. SI was in peak value on day 15 after irradiation in all groups and the SI value of SVPV treated group was higher than control group (p < 0.05). Our results suggest that SVP may be valuable natural peptides that relieve myelosuppression caused by radiation. The effect of SVP accelerating the hematopoietic recovery was potentially through a mechanism of stimulating the release of cytokines.
Dong
Weihua
W
Department of Pathophysiology, Guangzhou Medical College, Guangzhou, Guangdong, 510082, China. whdong@yahoo.cn
Wang
Lina
L
Kong
Tianhan
T
He
Yanjie
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D055014:Granulocyte-Macrophage Progenitor Cells / Q000187:drug effects / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D053582:Interleukin-1alpha / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D038042:Myelopoiesis / Q000187:drug effects* / Q000528:radiation effects; D009929:Organ Size / Q000187:drug effects / Q000528:radiation effects; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D012604:Scorpion Venoms / Q000378:metabolism*; D013154:Spleen / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D019089:Stem Cell Factor / Q000097:blood; D013950:Thymus Gland / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D013997:Time Factors; D014916:Whole-Body Irradiation
D053582:Interleukin-1alpha; D015850:Interleukin-6; D010455:Peptides; D012604:Scorpion Venoms; D019089:Stem Cell Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor