Molecular typing of Escherichia coli strains isolated from food in Casablanca (Morocco).
Serotyping of O- and H- antigens is regarded as the gold standard in classification of E. coli for taxonomic and epidemiological purposes similar to the Kaufmann-White scheme for Salmonella enterica. Molecular methods to replace or to support the serotyping were recently applied. Using the molecular polymorphism of the somatic (O- antigen) gene rfb cluster and flagella (H- antigen) gene fliC, 74 E. coli strains carrying the virulent genes isolated from food were characterised by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that 49 (66%) of the isolates revealed a reproducible and clear cut classification, with a very good correlation to the collection of reference strains, were found.
Badri
S
S
Institut Pasteur du Maroc Laboratoire de Microbiologie et Hygiène des Aliments et de l'Environnement, Casablanca, Maroc. samira_bdm@yahoo.fr
Fassouane
A
A
Filliol
I
I
Hassar
M
M
Cohen
N
N
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics*; D005408:Flagellin; D005516:Food Microbiology; D009018:Morocco; D005810:Multigene Family; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; C079397:rfb protein, Bacteria; D005408:Flagellin; C024413:endodeoxyribonuclease MboII; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases