Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens.
Chlamydophila pneumoniae is an atypical bacterial respiratory pathogen that is responsible for approximately 3-10% of community-acquired pneumonia cases. We report the evaluation of two distinct real-time PCR assays for rapid and specific detection of C. pneumoniae. We tested 401 clinical specimens, finding 5.7% positive, and confirmed a localized outbreak.
Mitchell
Stephanie L
SL
Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Budhiraja
Sona
S
Thurman
Kathleen A
KA
Lanier Thacker
W
W
Winchell
Jonas M
JM
D023521:Chlamydophila Infections / Q000175:diagnosis / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D015345:Oligonucleotide Probes; D018410:Pneumonia, Bacterial / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes