Determination of chloramphenicol in urine, feed water, milk and honey samples using molecular imprinted polymer clean-up.
A method for determination of low concentrations of chloramphenicol in urine, feed water, milk and honey was developed. A comparison was carried out between a routinely used analytical method based on solid phase extraction (SPE-C18) for cleaning the extract and the new procedure for the sample preparation using columns based on the molecular imprinted polymers (MIP) principle. The extracts obtained from the MIP clean-up procedure were clean enough for chromatografic analyses. Confirmatory analyses were conducted using GC/MS-NCI after derivatisation (silylation). The described method was fully validated according to CD 2002/657/EC. This method is considerably robust and allows very dirty samples to be processed. The described MIP procedure is very simple and low-time-consuming, and provides high throughput of the samples examined. This could be used for routine screening and confirmatory analyses as well.
Rejtharová
Martina
M
Institute for State Control of Veterinary Biologicals and Medicaments, CZ 621 00 Brno, Czech Republic. rejtharova@uskvbl.cz
Rejthar
Libor
L
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000652:urine; D002417:Cattle; D002701:Chloramphenicol / Q000652:urine*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D006722:Honey / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D013552:Swine; D014867:Water / Q000032:analysis*
D000900:Anti-Bacterial Agents; D011108:Polymers; D014867:Water; D002701:Chloramphenicol