title: Establishment of a primary culture method for mouse intestinal epithelial cells by organ culture of fetal small intestine.
abstract: Studies of the physiological functions of intestinal epithelial cells (IECs) have been limited by the difficulty of primary culture of IEC. We established a method for primary culture of mouse IEC by culturing fragments of fetal small intestines pretreated with EDTA. This method reproducibly resulted in the expansion of cytokeratin-positive epithelial cells, and vigorous expansion of the epithelial cells was observed only from intestinal fragments of embryonic days 15-16. These cells expressed alkaline phosphatase activity and major histocompatibility complex (MHC) class II molecules, indicating the mature phenotype of IEC in a small intestine. The cells also presented antigens to CD4(+) T cells. Furthermore, the cells expressed various cytokines and chemokines, and the expression was enhanced by bacterial stimulation. These results indicate that the primary-cultured mouse IEC prepared by the method established here can be a beneficial tool in study of the functions of IECs, especially in mucosal immunity.
authors: Department of Applied Biological Chemistry, The University of Tokyo, Japan.
authors: Kiyoshi
authors: K
authors: Yamada
authors: Kanako
authors: K
authors: Sato
authors: Satoru
authors: S
authors: Morishita
authors: Shuichi
authors: S
authors: Kaminogawa
authors: Mamoru
authors: M
authors: Totsuka
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D018925:Chemokines / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology* / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D005333:Fetus; D005786:Gene Expression Regulation / Q000276:immunology; D007421:Intestine, Small; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009924:Organ Culture Techniques; D010455:Peptides / Q000276:immunology; D011247:Pregnancy
chemical_list: D015415:Biomarkers; D018925:Chemokines; D008070:Lipopolysaccharides; D010455:Peptides