An improved method to knock out the asd gene of Salmonella enterica serovar Pullorum.
An asd-deleted (Deltaasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene. Based on the balanced lethal host-vector system, the phenotype of the Deltaasd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.
Geng
Shi-Zhong
SZ
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Jiao
Xin-An
XA
Pan
Zhi-Ming
ZM
Chen
Xiao-Juan
XJ
Zhang
Xiao-Ming
XM
Chen
Xiang
X
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D055786:Gene Knockout Techniques / Q000379:methods*; D009154:Mutation; D016133:Polymerase Chain Reaction; D019779:Salmonella enterica / Q000235:genetics* / Q000254:growth & development
D001426:Bacterial Proteins