title: Antimicrobial susceptibility, beta-lactamase and enterotoxin production in Bacillus cereus isolates from clinical and food samples.
abstract: The antimicrobial susceptibility of 30 clinical and 30 food Bacillus cereus isolates was determined. All isolates were susceptible to streptomycin, ciprofloxacin and gentamicin, 90 % of them to clindamycin and vancomycin, and 67 % to erythromycin. All isolates were resistant to amoxicillin with clavulanic acid, ampicillin, cefotaxime, ciprofloxacin, cloxacillin, cefotaxime with clavulanic acid and penicillin. The MIC values (determined by E-tests) were 48-256 mg/L for ampicillin, 0.19-1.5 mg/L for gentamicin, 0.125-1.0 mg/L for clindamycin, 0.047-4.0 mg/L for erythromycin and 1.5-16 mg/L for vancomycin. The MICs 4.6-18.75 g/L were observed for penicillin using the microdilution method. The presence of metallo-beta-lactamases was detected by E-test for 100 % of strains. Nonhemolytic diarrheal enterotoxin (NHE) was produced by 98.3 % of strains, while 31.7 % of them produced hemolytic diarrheal enterotoxin (HBL). Clinical isolates produced 10 % more HBL than food isolates. The psychrotrophic strains isolated from food samples produced NHE at 6.5 degrees C in 73 % of cases.
authors: Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia. karmen.torkar@vsz.uni-lj.si
authors: K
authors: K
authors: Godic Torkar
authors: K
authors: K
authors: Seme
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D024901:Drug Resistance, Multiple, Bacterial; D004768:Enterotoxins / Q000378:metabolism*; D005506:Food Contamination; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D013696:Temperature; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004768:Enterotoxins; D001618:beta-Lactamases