title: Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism.
abstract: The venom proteomes of Bothrops atrox from Colombia, Brazil, Ecuador, and Perú were characterized using venomic and antivenomic strategies. Our results evidence the existence of two geographically differentiated venom phenotypes. The venom from Colombia comprises at least 26 different proteins belonging to 9 different groups of toxins. PI-metalloproteinases and K49-PLA(2) molecules represent the most abundant toxins. On the other hand, the venoms from Brazilian, Ecuadorian, and Peruvian B. atrox contain predominantly PIII-metalloproteinases. These toxin profiles correlate with the venom phenotypes of adult and juvenile B. asper from Costa Rica, respectively, suggesting that paedomorphism represented a selective trend during the trans-Amazonian southward expansion of B. atrox through the Andean Corridor. The high degree of crossreactivity of a Costa Rican polyvalent (Bothrops asper, Lachesis stenophrys, Crotalus simus) antivenom against B. atrox venoms further evidenced the close evolutionary kinship between B. asper and B. atrox. This antivenom was more efficient immunodepleting proteins from the venoms of B. atrox from Brazil, Ecuador, and Perú than from Colombia. Such behaviour may be rationalized taking into account the lower content of poorly immunogenic toxins, such as PLA(2) molecules and PI-SVMPs in the paedomorphic venoms. The immunological profile of the Costa Rican antivenom strongly suggests the possibility of using this antivenom for the management of snakebites by B. atrox in Colombia and the Amazon regions of Ecuador, Perú and Brazil.
authors: Programa de Ofidismo/Escorpionismo, Universidad de Antioquia, Medellín 1226, Colombia; Escuela de Microbiología, Universidad de Antioquia, Medellín 1226, Colombia.
authors: Vitelbina
authors: V
authors: Núñez
authors: Pedro
authors: P
authors: Cid
authors: Libia
authors: L
authors: Sanz
authors: Pilar
authors: P
authors: De La Torre
authors: Yamileth
authors: Y
authors: Angulo
authors: Bruno
authors: B
authors: Lomonte
authors: José María
authors: JM
authors: Gutiérrez
authors: Juan J
authors: JJ
authors: Calvete
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000997:Antivenins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D017837:Bothrops / Q000378:metabolism*; D001938:Brazil; D003105:Colombia; D003364:Costa Rica; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D004484:Ecuador; D005843:Geography; D010568:Peru; D010641:Phenotype; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D012911:Snakes / Q000276:immunology / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000997:Antivenins; D003435:Crotalid Venoms; D020543:Proteome