Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity.
Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.
Yuki
Yoshikazu
Y
The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. yukiy@ims.u-tokyo.ac.jp
Tokuhara
Daisuke
D
Nochi
Tomonori
T
Yasuda
Hiroshi
H
Mejima
Mio
M
Kurokawa
Shiho
S
Takahashi
Yuko
Y
Kataoka
Nobuhiro
N
Nakanishi
Ushio
U
Hagiwara
Yukari
Y
Fujihashi
Kohtaro
K
Takaiwa
Fumio
F
Kiyono
Hiroshi
H
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012275:Oryza / Q000235:genetics / Q000276:immunology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D002772:Cholera Toxin