title: Residual structures in the acid-unfolded states of Vlambda6 proteins affect amyloid fibrillation.
abstract: Many proteins form amyloid-like fibrils in vitro under partially or highly unfolding conditions. Recently, we showed that the residual structure in highly unfolded state is closely related to amyloid fibril formation in hen lysozyme. Thus, to better understand the role of the residual structure on amyloid fibril formation, we focused on AL amyloidosis, which results from the extracellular deposition of monoclonal immunoglobulin light-chain variable domains (V(L)s) as insoluble fibrils. We examined the relationship between the residual structure and amyloid fibril formation on three lambda6 recombinant V(L) (rVlambda6) proteins, wild type, Jto, and Wil. Although rVlambda6 proteins are highly unfolded in pH 2, (15)N NMR transverse relaxation experiments revealed nonrandom structures in regions, which include some hydrophobic residues and a single disulfide bond, indicating the existence of residual structure in rVlambda6 proteins. However, the residual structure of Wil was markedly disrupted compared with those of the other proteins, despite there being no significant differences in amino acid sequences. Fibrillation experiments revealed that Wil had a longer lag time for fibril formation than the others. When the single disulfide bond was reduced and alkylated, the residual structure was largely disrupted and fibril formation was delayed in all three rVlambda6 proteins. It was suggested that the residual structure in highly unfolded state has a crucial role in amyloid fibril formation in many proteins, even pathogenic ones.
authors: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors: Tomonori
authors: T
authors: Mishima
authors: Takatoshi
authors: T
authors: Ohkuri
authors: Akira
authors: A
authors: Monji
authors: Takaaki
authors: T
authors: Kanemaru
authors: Yoshito
authors: Y
authors: Abe
authors: Tadashi
authors: T
authors: Ueda
mesh_terms: D000143:Acids / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007146:Immunoglobulin lambda-Chains / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D017510:Protein Folding / Q000187:drug effects*; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D011994:Recombinant Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D000143:Acids; D000682:Amyloid; D007146:Immunoglobulin lambda-Chains; D011994:Recombinant Proteins