Pseudofulvimonas gallinarii gen. nov., sp. nov., a new member of the family Xanthomonadaceae.
A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming bacterium (Sa15(T)) was isolated from the air in a duck barn. On the basis of 16S rRNA gene sequence similarity studies, the organism was grouped into the class gammaproteobacteria in the neighbourhood of the genus Fulvimonas. The quinone system consisted exclusively of ubiquinone Q-8. The polar lipid profile was mainly composed of the major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidylcholine, and moderate amounts of phosphatidylglycerol and an unidentified lipid. This profile was substantially different from that of Fulvimonas soli LMG 19981(T) examined concurrently. The polyamine pattern showed the predominant amine spermidine. Major fatty acids (iso-C(15 : 0), iso-C(17 : 1)omega9c and iso-C(17 : 0)) were in agreement with its phylogenetic affiliation in the vicinity of Fulvimonas; however, differences in the polar lipid and fatty acid patterns and the polyamine profiles could be observed as well. On the basis of DNA-DNA pairing results, chemotaxonomic data and physiological and biochemical data, the strain can be clearly differentiated from Fulvimonas soli. It is evident that this organism represents a novel genus, for which the name Pseudofulvimonas gallinarii gen. nov., sp. nov. is proposed, with the type strain Sa15(T) (=DSM 21944(T) =CCM 7599(T)).
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Martin
Elena
E
Bundesanstalt für Arbeitschutz und Arbeitsmedizin, D-10317 Berlin, Germany.
Lodders
Nicole
N
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Langer
Stefan
S
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, D-38124 Braunschweig, Germany.
Jäckel
Udo
U
Bundesanstalt für Arbeitschutz und Arbeitsmedizin, D-10317 Berlin, Germany.
Busse
Hans-Jürgen
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004372:Ducks / Q000382:microbiology; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000378:metabolism; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D044166:Xanthomonadaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial; D008055:Lipids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S