The control of cell adhesion and detachment on thin films of thermoresponsive poly[(N-isopropylacrylamide)-r-((3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)ammonium hydroxide)].
This paper describes the formation of poly(N-isopropylacrylamide) (PNIPAAm) and poly[(N-isopropylacrylamide)-r-((3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)ammonium hydroxide)] (P(NIPAAm-r-MPDSAH)) films on a glass surface via surface-initiated, atom transfer radical polymerization as a cell-culture platform. The films of PNIPAAm with various thicknesses and of P(NIPAAm-r-MPDSAH) with various ratios of NIPAAm and MPDSAH are formed to investigate the behaviors of cell adhesion and detachment. In the case of the PNIPAAm-grafted glass surfaces, the optimal film thickness, achieving the effective control of both cell adhesion and detachment, is estimated to be 11-13 nm for NIH 3T3 fibroblast cells. The adhesion and detachment behaviors of NIH 3T3 fibroblast cells are further tuned by incorporating the hydrophilic and non-biofouling MPDSAH moiety into the PNIPAAm system. The cell adhesion and detachment are controlled best, when the ratio of NIPAAm and MPDSAH is 75:1 (NIPAAm:MPDSAH).
Kong
Bokyung
B
Department of Chemistry, KAIST, Daejeon 305-701, South Korea.
Choi
Joon S
JS
Jeon
Saewha
S
Choi
Insung S
IS
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D008422:Materials Testing; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011108:Polymers / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013696:Temperature
D000178:Acrylamides; D000180:Acrylic Resins; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D008689:Methacrylates; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C520687:poly((3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)ammonium hydroxide); C052970:poly-N-isopropylacrylamide