2,6,8-Trisubstituted 3-hydroxychromone derivatives as fluorophores for live-cell imaging.
We present the synthesis and photophysical characterisation of a series of structurally diverse, fluorescent 2,6,8-trisubstituted 3-hydroxychromone derivatives with high fluorescence quantum yields and molar extinction coefficients. Two of these derivatives (9 and 10 a) have been studied as fluorophores for cellular imaging in HeLa cells and show excellent permeability and promising fluorescence properties in a cellular environment. In addition, we have demonstrated by photophysical characterisation of 3-isobutyroxychromone derivatives that esterification of the 3-hydroxyl group results in acceptable and useful fluorescence properties.
Dyrager
Christine
C
Department of Chemistry-Medicinal Chemistry, University of Gothenburg, 412 96 Göteborg, Sweden.
Friberg
Annika
A
Dahlén
Kristian
K
Fridén-Saxin
Maria
M
Börjesson
Karl
K
Wilhelmsson
L Marcus
LM
Smedh
Maria
M
Grøtli
Morten
M
Luthman
Kristina
K
D002867:Chromones / Q000138:chemical synthesis / Q000737:chemistry*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D010777:Photochemistry
C479534:3-hydroxychromone; D002867:Chromones; D005456:Fluorescent Dyes