Deinococcus aetherius sp. nov., isolated from the stratosphere.
A pink-red pigmented, non-motile, coccoid bacterial strain, ST0316(T), was isolated from dust samples collected from the stratosphere in Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that it belonged to the genus Deinococcus. DNA G+C content (69.8 mol%), desiccation tolerance, and resistance to gamma-rays [D(10) (dose required to reduce the bacterial population by 10-fold) >8 kGy] and UV radiation (D(10) 1000 J m(-2)) supported the affiliation of strain ST0316(T) to the genus Deinococcus. The major peptidoglycan amino acids were d-glutamic acid, glycine, d-alanine, l-alanine and ornithine. Predominant fatty acids were C(16 : 1)omega7c, C(16 : 0), C(17 : 0) and iso-C(17 : 0). Strain ST0316(T) diverged from recognized species of the genus Deinococcus, showing less than 93.0 % similarity values to its closest relatives Deinococcus apachensis, D. aerius, D. geothermalis and D. murrayi. Strain ST0316(T) also differed from the type strains of closely related species in its polar lipid profile, nitrate reduction and carbon-source assimilation tests. Therefore, we propose a new species of the genus Deinococcus, Deinococcus aetherius sp. nov. (type strain, ST0316(T) =JCM 11751(T) =DSM 21230(T)).
Yang
Yinjie
Y
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
Itoh
Takashi
T
Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Yokobori
Shin-Ichi
SI
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
Shimada
Haruo
H
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
Itahashi
Shiho
S
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
Satoh
Katsuya
K
Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
Ohba
Hirofumi
H
Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
Narumi
Issay
I
Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
Yamagishi
Akihiko
A
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
D000391:Air Microbiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004391:Dust / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D005720:Gamma Rays; D020459:Genes, rRNA; D007564:Japan; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004275:DNA, Ribosomal; D004391:Dust; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S