A cold-induced thioredoxin h of rice, OsTrx23, negatively regulates kinase activities of OsMPK3 and OsMPK6 in vitro.
Cytosolic thioredoxins are small conserved proteins that are involved in cellular redox regulation. Here, we report that a major and cold-induced thioredoxin h of rice, OsTrx23, has an inhibitory activity on stress-activated mitogen-activated protein kinases (MAPKs), OsMPK3 and OsMPK6 in vitro. This inhibition effects were redox-dependent and did not involve stable physical interaction. The data suggested a novel mechanism for redox regulation of MAPKs in plants.
Xie
Guosheng
G
National Agricultural Research Center for Hokkaido Region, National Agricultural and Food Research Organization, Sapporo, Japan.
Kato
Hideki
H
Sasaki
Kentaro
K
Imai
Ryozo
R
D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics; D003080:Cold Temperature; D048371:MAP Kinase Kinase 3 / Q000235:genetics / Q000378:metabolism*; D048669:MAP Kinase Kinase 6 / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D054480:Thioredoxin h / Q000145:classification / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D054480:Thioredoxin h; D048371:MAP Kinase Kinase 3; D048669:MAP Kinase Kinase 6