Hippocampal protein levels related to spatial memory are different in the Barnes maze and in the multiple T-maze.
The Multiple T-maze (MTM) and the Barnes maze (BM) are land mazes used for the evaluation of spatial memory. The observation that mice are performing differently in individual mazes made us test the hypothesis that differences in cognitive performances in the two land mazes would be accompanied by differences in hippocampal protein levels. C57BL/6J mice were tested in the BM and in the MTM, hippocampi were extirpated 6 h following the probe trials each, and proteins were extracted for gel-based proteomic analysis. Mice learned the task in both paradigms. Levels of hippocampal proteins from several pathways including signaling, chaperone, and metabolic cascades were significantly different between the two spatial memory tasks. Protein levels were linked to spatial memory specifically as yoked controls were used.
Zheng
Jun-Fang
JF
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
Patil
Sudarshan S
SS
Chen
Wei-Qiang
WQ
An
Wei
W
He
Jun-Qi
JQ
Höger
Harald
H
Lubec
Gert
G
D000818:Animals; D019703:Calcineurin / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D018782:Maze Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology*; D013028:Space Perception / Q000502:physiology; D018709:Statistics, Nonparametric
D011506:Proteins; D019703:Calcineurin