Preliminary investigation on the action modes of an oligosaccharide-producing multifunctional amylase.
The action modes of an oligosaccharide-producing multifunctional amylase (OPMA) were investigated using glucose and some oligosaccharides as its substrates. OPMA did not cause the hydrolysis of maltose or isomaltose, but it catalyzed the alpha-1,6-transglycosylation of maltose to produce isomaltose or did the self-condensation of isomaltose to form isomaltotetraose and 4-O-alpha-isomaltosyl isomaltose. OPMA exhibited strong alpha-1,6-transglycosylation activity in addition to its alpha-1,4-hydrolytic activity on higher oligosaccharides substrates rather than bisaccharides. OPMA displayed high acceptor specificity in its transglycosylation or condensation reaction. OPMA seemed to only take glucose or isomaltose as the acceptor molecule in its transglycosylation or condensation reaction, which made glucose or isomaltose form higher products, and as a result, glucose or isomaltose were absent in the final products. In view of the simultaneously formation of several transglycosylation or condensation products, it was predicted that there might be separate donor and acceptor sites in OPMA's active center and the fact that the catalytically active form of this enzyme included its homodimer or homotrimer supported this prediction. Accordingly, a special pathway, isomaltose pathway, for OPMA catalysis was proposed to emphasize the central or important signification of isomaltose in OPMA catalysis.
Wang
Yang
Y
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130021, People's Republic of China.
Li
Fan
F
Zhang
Yingjiu
Y
D000681:Amylases / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D004789:Enzyme Activation; D005947:Glucose / Q000737:chemistry; D006031:Glycosylation; D007534:Isomaltose / Q000138:chemical synthesis / Q000737:chemistry; D008320:Maltose / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*
D009844:Oligosaccharides; D007534:Isomaltose; D008320:Maltose; D000681:Amylases; D005947:Glucose