Seed viability and functional properties of broccoli sprouts during germination and postharvest storage as affected by irradiation of seeds.
The viability of broccoli seeds and functional properties, such as ascorbic acid, carotenoid, chlorophyll, and total phenol contents, of broccoli sprouts grown from irradiated seeds were evaluated. The seeds were irradiated using electron beam and gamma ray at doses up to 8 kGy. High germination percentages (>90%) were observed in seeds irradiated at < or =4 kGy, but the yield ratio and sprout length decreased with increased irradiation dose. Irradiation at > or =6 kGy resulted in curling of the sprout roots. Germinated seeds contained higher amounts of nutrients than raw seeds but the nutritional quality of sprouts decreased during postharvest storage. Radiation treatment hampered the growth of irradiated seeds resulting in underdeveloped sprouts with decreased ascorbic acid, carotenoid, and chlorophyll contents. In addition, the decrease in functional content of sprouts was more substantial in samples grown from high-dose (5 kGy) irradiated seeds than that of the low-dose (1 kGy) treated ones. Seed irradiation did not negatively affect the total phenol content of sprouts. In general, electron beam and gamma irradiation of broccoli seeds showed similar effects on the viability and functional properties of sprouts.
Waje
Catherine K
CK
Dept. of Food Science and Technology, Kyungpook Natl. Univ., Daegu, Korea.
Jun
So-Yun
SY
Lee
Yeon-Kyung
YK
Moon
Kwang-Deog
KD
Choi
Yong Hee
YH
Kwon
Joong-Ho
JH
D001205:Ascorbic Acid / Q000528:radiation effects; D001937:Brassica / Q000502:physiology / Q000528:radiation effects*; D002338:Carotenoids / Q000528:radiation effects; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D002734:Chlorophyll / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005511:Food Handling / Q000379:methods*; D005514:Food Irradiation / Q000379:methods*; D018525:Germination / Q000502:physiology / Q000528:radiation effects*; D009753:Nutritive Value; D010636:Phenols / Q000528:radiation effects; D012639:Seeds / Q000502:physiology / Q000528:radiation effects*
D010636:Phenols; D002734:Chlorophyll; D002338:Carotenoids; D001205:Ascorbic Acid