title: Deinococcus aetherius sp. nov., isolated from the stratosphere.
abstract: A pink-red pigmented, non-motile, coccoid bacterial strain, ST0316(T), was isolated from dust samples collected from the stratosphere in Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that it belonged to the genus Deinococcus. DNA G+C content (69.8 mol%), desiccation tolerance, and resistance to gamma-rays [D(10) (dose required to reduce the bacterial population by 10-fold) >8 kGy] and UV radiation (D(10) 1000 J m(-2)) supported the affiliation of strain ST0316(T) to the genus Deinococcus. The major peptidoglycan amino acids were d-glutamic acid, glycine, d-alanine, l-alanine and ornithine. Predominant fatty acids were C(16 : 1)omega7c, C(16 : 0), C(17 : 0) and iso-C(17 : 0). Strain ST0316(T) diverged from recognized species of the genus Deinococcus, showing less than 93.0 % similarity values to its closest relatives Deinococcus apachensis, D. aerius, D. geothermalis and D. murrayi. Strain ST0316(T) also differed from the type strains of closely related species in its polar lipid profile, nitrate reduction and carbon-source assimilation tests. Therefore, we propose a new species of the genus Deinococcus, Deinococcus aetherius sp. nov. (type strain, ST0316(T) =JCM 11751(T) =DSM 21230(T)).
authors: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
authors: Yinjie
authors: Y
authors: Yang
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Takashi
authors: T
authors: Itoh
authors: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
authors: Shin-Ichi
authors: SI
authors: Yokobori
authors: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
authors: Haruo
authors: H
authors: Shimada
authors: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
authors: Shiho
authors: S
authors: Itahashi
authors: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
authors: Katsuya
authors: K
authors: Satoh
authors: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
authors: Hirofumi
authors: H
authors: Ohba
authors: Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
authors: Issay
authors: I
authors: Narumi
authors: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
authors: Akihiko
authors: A
authors: Yamagishi
mesh_terms: D000391:Air Microbiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004391:Dust / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D005720:Gamma Rays; D020459:Genes, rRNA; D007564:Japan; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D004391:Dust; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S