title: The adjuvant effect of ambient particulate matter is closely reflected by the particulate oxidant potential.
abstract: BACKGROUND
  It has been demonstrated that ambient particulate matter (PM) can act as an adjuvant for allergic sensitization. Redox-active organic chemicals on the particle surface play an important role in PM adverse health effects and may determine the adjuvant effect of different particle types according to their potential to perturb redox equilibrium in the immune system.
  
  
  OBJECTIVE
  We determined whether the adjuvant effect of ambient fine particles versus ultrafine particles (UFPs) is correlated to their prooxidant potential.
  
  
  METHODS
  We have established an intranasal sensitization model that uses ambient PM as a potential adjuvant for sensitization to ovalbumin (OVA), which enhances the capacity for secondary OVA challenge to induce allergic airway inflammation.
  
  
  RESULTS
  UFPs with a greater polycyclic aromatic hydrocarbon (PAH) content and higher oxidant potential enhanced OVA sensitization more readily than did fine particles. This manifests as enhanced allergic inflammation upon secondary OVA challenge, leading to eosinophilic inflammation and mucoid hyperplasia starting at the nasal turbinates all the way down to the small pulmonary airways. The thiol antioxidant N-acetyl cysteine was able to suppress some of these sensitization events.
  
  
  CONCLUSIONS
  The adjuvant effects of ambient UFP is determined by their oxidant potential, which likely plays a role in changing the redox equilibrium in the mucosal immune system.
authors: Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors: Ning
authors: N
authors: Li
authors: Meiying
authors: M
authors: Wang
authors: Lori A
authors: LA
authors: Bramble
authors: Debra A
authors: DA
authors: Schmitz
authors: James J
authors: JJ
authors: Schauer
authors: Constantinos
authors: C
authors: Sioutas
authors: Jack R
authors: JR
authors: Harkema
authors: Andre E
authors: AE
authors: Nel
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000378:metabolism; D009666:Nose / Q000187:drug effects; D010047:Ovalbumin / Q000276:immunology*; D018384:Oxidative Stress / Q000187:drug effects; D052638:Particulate Matter / Q000276:immunology* / Q000633:toxicity*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D052638:Particulate Matter; D010047:Ovalbumin