Notch2 expression is decreased in colorectal cancer and related to tumor differentiation status.
BACKGROUND
The significance of Notch2 expression in colorectal cancer (CRC) has not been clearly investigated. We investigated the expression of Notch2 and its relationship with differentiation status and tumor stage by studying clinical CRC specimens with matched adjacent normal tissues and normal control colorectal specimens.


METHODS
Immunohistochemistry, real-time polymerase chain reaction, and Western blot analysis were performed to assess the expression of Notch2 in clinical CRC specimens. Also, Notch2 levels in an induced differentiation model of CRC cell lines were investigated.


RESULTS
It was found that Notch2 expression was decreased in cancer tissues compared with adjacent normal tissue and normal control tissues. Also, a tendency for decreased expression was observed when going from well to poorly differentiated carcinomas, as well as going from tumor, node, metastasis system stage I to stage IV. With the differentiation of colon cancer cells, the expression of Notch2 increased. To support this observation, colon cancer cell lines HT29 and SW620 were induced to differentiate in culture, and expression of Notch2 was investigated. A clear increase expression of Notch2 was observed.


CONCLUSIONS
Notch2 expression correlated closely with CRC and may play a role in tumor inhibition in colon carcinogenesis.
Chu
Dake
D
The State Key Laboratory of Cancer Biology, Department of Gastrointestinal Surgery, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, China.
Zheng
Jianyong
J
Wang
Weizhong
W
Zhao
Qingchuan
Q
Li
Yunming
Y
Li
Jipeng
J
Xie
Huahong
H
Zhang
Hongwei
H
Dong
Guanglong
G
Xu
Chunsheng
C
Li
Mengbin
M
Chen
Dongli
D
Ji
Gang
G
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D002454:Cell Differentiation; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051883:Receptor, Notch2 / Q000235:genetics / Q000378:metabolism*; D012007:Rectum / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; C497764:NOTCH2 protein, human; D012333:RNA, Messenger; D051883:Receptor, Notch2