Antiviral activity of the hemolymph of Lonomia obliqua (Lepidoptera: Saturniidae).
Potent antiviral activity against measles, influenza and polio viruses was observed in the hemolymph of Lonomia obliqua. The antiviral protein responsible for this activity was isolated, purified by gel filtration chromatography using a gel filtration column system (Superdex 75) and further fractionated using a Resource-Q ion exchange column system. Experiments with the purified protein led to a 157-fold reduction (from 3.3+/-1.25 x 10(7) to 2.1+/-1.5 x 10(5) TCID(50)mL(-1)) in measles virus production and a 61-fold reduction (from 2.8+/-1.08 x 10(9) to 4.58+/-1.42 x 10(7)mL(-1)) in polio virus production. Heating and freezing seem to have no influence over its antiviral activity. Also, the protein does not display virucidal activity and does not act on receptors on the cell membrane. The observations suggest an intracellular mechanism of action and that the protein may act as a constitutive agent that affects the innate antiviral immune response.
Greco
Katia N
KN
Laboratório de Parasitologia e Entomologia, Instituto Butantan, São Paulo, Brazil.
Mendonça
Rita Maria Z
RM
Moraes
Roberto H P
RH
Mancini
Dalva A P
DA
Mendonça
Ronaldo Z
RZ
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004285:Dogs; D006458:Hemolymph / Q000737:chemistry / Q000276:immunology*; D019476:Insect Proteins / Q000276:immunology* / Q000302:isolation & purification; D009036:Moths / Q000737:chemistry* / Q000276:immunology*; D055550:Protein Stability; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects; D014780:Viruses / Q000187:drug effects
D019476:Insect Proteins