Partial resistance of Medicago truncatula to Aphanomyces euteiches is associated with protection of the root stele and is controlled by a major QTL rich in proteasome-related genes.
A pathosystem between Aphanomyces euteiches, the causal agent of pea root rot disease, and the model legume Medicago truncatula was developed to gain insights into mechanisms involved in resistance to this oomycete. The F83005.5 French accession and the A17-Jemalong reference line, susceptible and partially resistant, respectively, to A. euteiches, were selected for further cytological and genetic analyses. Microscopy analyses of thin root sections revealed that a major difference between the two inoculated lines occurred in the root stele, which remained pathogen free in A17. Striking features were observed in A17 roots only, including i) frequent pericycle cell divisions, ii) lignin deposition around the pericycle, and iii) accumulation of soluble phenolic compounds. Genetic analysis of resistance was performed on an F7 population of 139 recombinant inbred lines and identified a major quantitative trait locus (QTL) near the top of chromosome 3. A second study, with near-isogenic line responses to A. euteiches confirmed the role of this QTL in expression of resistance. Fine-mapping allowed the identification of a 135-kb sequenced genomic DNA region rich in proteasome-related genes. Most of these genes were shown to be induced only in inoculated A17. Novel mechanisms possibly involved in the observed partial resistance are proposed.
Djébali
Naceur
N
Université de Toulose, Toulose, France.
Jauneau
Alain
A
Ameline-Torregrosa
Carine
C
Chardon
Fabien
F
Jaulneau
Valérie
V
Mathé
Catherine
C
Bottin
Arnaud
A
Cazaux
Marc
M
Pilet-Nayel
Marie-Laure
ML
Baranger
Alain
A
Aouani
Mohamed Elarbi
ME
Esquerré-Tugayé
Marie-Thérèse
MT
Dumas
Bernard
B
Huguet
Thierry
T
Jacquet
Christophe
C
D044744:Aphanomyces / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate / Q000235:genetics; D007178:Inbreeding; D046913:Medicago truncatula / Q000166:cytology / Q000235:genetics / Q000382:microbiology*; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000382:microbiology*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D015203:Reproducibility of Results
D046988:Proteasome Endopeptidase Complex