Polypyrrole-peptide microarray for biomolecular interaction analysis by SPR imaging.
Nowadays, high-throughput analysis of biological events is a great challenge which could take benefit of the recent development of microarray devices. The great potential of such technology is related to the availability of a chip bearing a large set of probes, stable and easy to obtain, and suitable for ligand-binding detection. Here, we describe a new method based on polypyrrole chemistry, allowing the covalent immobilization of peptides in a microarray format and on a gold surface compatible with the use of surface plasmon resonance. This technique is then illustrated by the detection and characterization of antibodies induced by hepatitis C virus and present in patients' serums.
Villiers
Marie-Bernadette
MB
Inserm, U823, IAPC, Grenoble, France.
Cortès
Sandra
S
Brakha
Carine
C
Marche
Patrice
P
Roget
André
A
Livache
Thierry
T
D000818:Animals; D000906:Antibodies / Q000032:analysis; D006801:Humans; D007163:Immunosorbent Techniques; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D025941:Protein Interaction Mapping / Q000295:instrumentation / Q000379:methods*; D011758:Pyrroles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D000906:Antibodies; D010455:Peptides; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole