[Determination the chemical speciation of Cu, Zn, Fe and Mn in Radix Scutellariae by AAS].
An analysis method was developed to determine the chemical speciation of Cu, Zn, Fe and Mn in radix scutellariae decoction using atomic absorption spectroscopy(AAS). The decoction can be divided into suspension and soluble species by 0.45 microm filter membrane and the soluble species can be separated into organism and inorganic species by LSA-10 macroporous resin. These elements in water-soluble test samples can be divided into alcohol-soluble and water-soluble by adopting n-octyl alcohol-water allocation system in man-made gastric acidity. Then, the concentration of these elements was determined by AAS, which provided more chemical speciation information about these elements instead of the total amount of them only in radix scutellariae. Deteotion limit of Cu, Zn and Mn by using the method was all 0.01 microg x mL(-1) and was 0.02 microg x mL(-1) for Fe. The RSD was in the range of 1.5%-3.6% (n=11) and the recovery rate of soluble species and inorganic species were in range of 96.7%-105.0%. The method has been successfully applied to determine the chemical speciation of Cu, Zn, Fe and Mn in radix scutellariae, which was very important for overall study of radix scutellariae.
Miao
Shan
S
Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032 China. miaoshan@fmmu.edu.cn
Sun
Ji-yuan
JY
Xie
Yan-hua
YH
Wang
Jian-bo
JB
Shi
Xiao-peng
XP
Ding
Yuan-yuan
YY
Bi
Lin-lin
LL
Gao
Shuang-bin
SB
Wang
Si-wang
SW
D003300:Copper / Q000032:analysis / Q000737:chemistry; D007501:Iron / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D057230:Limit of Detection; D008345:Manganese / Q000032:analysis / Q000737:chemistry; D019216:Metals, Heavy / Q000032:analysis* / Q000737:chemistry*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013054:Spectrophotometry, Atomic / Q000379:methods*; D015032:Zinc / Q000032:analysis / Q000737:chemistry
D019216:Metals, Heavy; D012997:Solvents; D008345:Manganese; D003300:Copper; D007501:Iron; D015032:Zinc