Discovery of small peptides derived from embryonic lethal abnormal vision proteins structure showing RNA-stabilizing properties.
Four peptides corresponding to highly conserved motives within the first two RNA recognition motif-type domains of ELAV proteins were prepared, and their effect on the stability of NOVA-1 and VEGF ELAV-target mRNAs was evaluated. Biological results show that in the presence of phorbol 12-myristate 13-acetate (a PKC activator triggering the ELAV pathway), an equimolar mixture of peptides induces a statistically significant stabilization of the selected transcripts, suggesting a synergic effect of the two stimuli.
Rossi
Daniela
D
Department of Pharmaceutical Chemistry, University of Pavia, Viale Taramelli 12, I-27100 Pavia, Italy.
Amadio
Marialaura
M
Carnevale Baraglia
Anna
A
Azzolina
Ornella
O
Ratti
Antonia
A
Govoni
Stefano
S
Pascale
Alessia
A
Collina
Simona
S
D020816:Amino Acid Motifs; D000951:Antigens, Neoplasm / Q000235:genetics*; D045744:Cell Line, Tumor; D017124:Conserved Sequence; D051959:ELAV Proteins / Q000737:chemistry*; D020536:Enzyme Activators / Q000494:pharmacology; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics*; D000090682:Neuro-Oncological Ventral Antigen; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D020871:RNA Stability / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D000951:Antigens, Neoplasm; D051959:ELAV Proteins; D020536:Enzyme Activators; D009419:Nerve Tissue Proteins; D000090682:Neuro-Oncological Ventral Antigen; D009842:Oligopeptides; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D042461:Vascular Endothelial Growth Factor A; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate