Phosphorylation-induced conformational changes in Rap1b: allosteric effects on switch domains and effector loop.
Rap1b has been implicated in the transduction of the cAMP mitogenic response. Agonists that increase intracellular cAMP rapidly activate (i.e. GTP binding) and phosphorylate Rap1b on Ser(179) at its C terminus. cAMP-dependent protein kinase (PKA)-mediated phosphorylation of Rap1b is required for cAMP-dependent mitogenesis, tumorigenesis, and inhibition of AKT activity. However, the role of phosphorylation still remains unknown. In this study, we utilized amide hydrogen/deuterium exchange mass spectroscopy (DXMS) to assess potential conformational changes and/or mobility induced by phosphorylation. We report here DXMS data comparing exchange rates for PKA-phosphorylated (Rap1-P) and S179D phosphomimetic (Rap1-D) Rap1b proteins. Rap1-P and Rap1-D behaved exactly the same, revealing an increased exchange rate in discrete regions along the protein; these regions include a domain around the phosphorylation site and unexpectedly the two switch loops. Thus, local effects induced by Ser(179) phosphorylation communicate allosterically with distal domains involved in effector interaction. These results provide a mechanistic explanation for the differential effects of Rap1 phosphorylation by PKA on effector protein interaction.
Edreira
Martin M
MM
Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
Li
Sheng
S
Hochbaum
Daniel
D
Wong
Sergio
S
Gorfe
Alemayehu A
AA
Ribeiro-Neto
Fernando
F
Woods
Virgil L
VL
Altschuler
Daniel L
DL
D000494:Allosteric Regulation; D000595:Amino Acid Sequence; D002460:Cell Line; D041961:Deuterium Exchange Measurement; D006801:Humans; D013058:Mass Spectrometry; D008958:Models, Molecular; D010766:Phosphorylation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D020668:rap GTP-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*
C116753:RAP1B protein, human; D020668:rap GTP-Binding Proteins