Screening non-coding RNAs in transcriptomes from neglected species using PORTRAIT: case study of the pathogenic fungus Paracoccidioides brasiliensis.
BACKGROUND
Transcriptome sequences provide a complement to structural genomic information and provide snapshots of an organism's transcriptional profile. Such sequences also represent an alternative method for characterizing neglected species that are not expected to undergo whole-genome sequencing. One difficulty for transcriptome sequencing of these organisms is the low quality of reads and incomplete coverage of transcripts, both of which compromise further bioinformatics analyses. Another complicating factor is the lack of known protein homologs, which frustrates searches against established protein databases. This lack of homologs may be caused by divergence from well-characterized and over-represented model organisms. Another explanation is that non-coding RNAs (ncRNAs) may be caught during sequencing. NcRNAs are RNA sequences that, unlike messenger RNAs, do not code for protein products and instead perform unique functions by folding into higher order structural conformations. There is ncRNA screening software available that is specific for transcriptome sequences, but their analyses are optimized for those transcriptomes that are well represented in protein databases, and also assume that input ESTs are full-length and high quality.


RESULTS
We propose an algorithm called PORTRAIT, which is suitable for ncRNA analysis of transcriptomes from poorly characterized species. Sequences are translated by software that is resistant to sequencing errors, and the predicted putative proteins, along with their source transcripts, are evaluated for coding potential by a support vector machine (SVM). Either of two SVM models may be employed: if a putative protein is found, a protein-dependent SVM model is used; if it is not found, a protein-independent SVM model is used instead. Only ab initio features are extracted, so that no homology information is needed. We illustrate the use of PORTRAIT by predicting ncRNAs from the transcriptome of the pathogenic fungus Paracoccidoides brasiliensis and five other related fungi.


CONCLUSIONS
PORTRAIT can be integrated into pipelines, and provides a low computational cost solution for ncRNA detection in transcriptome sequencing projects.
Arrial
Roberto T
RT
Biology Institute, University of Brasília, Brazil. rtarrial@gmail.com
Togawa
Roberto C
RC
Brigido
Marcelo de M
Mde M
D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D005822:Genetic Vectors; D010228:Paracoccidioides / Q000235:genetics*; D012331:RNA, Fungal / Q000032:analysis* / Q000235:genetics; D022661:RNA, Untranslated / Q000032:analysis* / Q000235:genetics; D012984:Software; D014158:Transcription, Genetic
D012331:RNA, Fungal; D022661:RNA, Untranslated