title: Quantum dot-based HIV capture and imaging in a microfluidic channel.
abstract: Globally, over 33.2 million people who mostly live in developing countries with limited access to the appropriate medical care suffer from the human immunodeficiency virus (HIV) infection. We developed an on-chip HIV capture and imaging method using quantum dots (Qdots) from fingerprick volume (10 microl) of unprocessed HIV-infected patient whole blood in anti-gp120 antibody-immobilized microfluidic chip. Two-color Qdots (Qdot525 and Qdot655 streptavidin conjugates) were used to identify the captured HIV by simultaneous labeling the envelope gp120 glycoprotein and its high-mannose glycans. This dual-stain imaging technique using Qdots provides a new and effective tool for accurate identification of HIV particles from patient whole blood without any pre-processing. This on-chip HIV capture and imaging platform creates new avenues for point-of-care diagnostics and monitoring applications of infectious diseases.
authors: Bio-Acoustic MEMS in Medicine Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, United States.
authors: Yun-Gon
authors: YG
authors: Kim
authors: Sangjun
authors: S
authors: Moon
authors: Daniel R
authors: DR
authors: Kuritzkes
authors: Utkan
authors: U
authors: Demirci
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology*; D005453:Fluorescence; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D045663:Quantum Dots; D013997:Time Factors
chemical_list: D055910:Antibodies, Immobilized; D015699:HIV Envelope Protein gp120