title: Expression of matrix metalloproteinases during impairment and recovery of the avian growth plate.
abstract: Tibial dyschondroplasia (TD) is a prevalent skeletal abnormality associated with rapid growth rate in many avian species. It is characterized by the presence of a nonvascularized, nonmineralized lesion that extends from the epiphyseal growth plate into the metaphysis of the proximal tibiotarsal bones. In this study, we examined the expression of 4 members of the matrix metalloproteinase (MMP) family (MMP-2, -3, -9, and -13) in thiram-induced TD lesions and in the process of recovery from TD, by in situ hybridization analysis and quantitative real-time PCR. A model for the induction and recovery of TD was established, consisting of 3 groups of broilers: (1) thiram group, chicks fed a thiram-enriched diet to induce TD; (2) recovery group, chicks fed a thiram-enriched diet during the first week of the experiment and a normal diet from the second week on; and (3) control group, chicks fed a normal diet throughout the experimental period. In agreement with our previous data, the 4 MMP were diminished in the TD lesion (P < 0.05); however, in the current study we show that the growth plate was able to repair itself and that the MMP reappeared during the process of recovery from TD. Our results strengthen the link between MMP expression and growth-plate impairment, and we suggest that gelatinase activity (MMP-2 and 9) facilitates this process.
authors: Department of Biochemistry and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Israel.
authors: H
authors: H
authors: Dan
authors: S
authors: S
authors: Simsa-Maziel
authors: A
authors: A
authors: Hisdai
authors: D
authors: D
authors: Sela-Donenfeld
authors: E
authors: E
authors: Monsonego Ornan
mesh_terms: D000818:Animals; D002645:Chickens / Q000254:growth & development*; D015971:Gene Expression Regulation, Enzymologic; D006132:Growth Plate / Q000201:enzymology* / Q000254:growth & development; D017403:In Situ Hybridization; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D009153:Mutagens; D010009:Osteochondrodysplasias / Q000201:enzymology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000201:enzymology*; D013893:Thiram / Q000494:pharmacology; D013977:Tibia / Q000201:enzymology / Q000254:growth & development
chemical_list: D009153:Mutagens; D013893:Thiram; D020782:Matrix Metalloproteinases; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2