Rat monoclonal antibodies specific for LST1 proteins.
The LST1 gene is located in the human MHC class III region and encodes transmembrane and soluble isoforms that have been suggested to play a role in the regulation of the immune response and are associated with inflammatory diseases such as rheumatoid arthritis. Here we describe the generation and characterization of the first monoclonal antibodies against LST1. Two hybridoma lines secreting monoclonal antibodies designated 7E2 and 8D12 were established. The 7E2 antibody detects recombinant and endogenous LST1 by Western blot analysis while 8D12 reacts with recombinant and endogenous LST1 in immunoprecipitation and flow cytometry procedures. The newly established antibodies were used to survey LST1 protein expression in human cell lines, which was found to be tightly regulated, allowing the expression of transmembrane isoforms but suppressing soluble isoforms.
Schiller
Christian
C
Department of Biology II, Ludwig-Maximilians-Universität München, Germany.
Nitschké
Maximilian J E
MJ
Seidl
Alexander
A
Kremmer
Elisabeth
E
Weiss
Elisabeth H
EH
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007249:Inflammation; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology*; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D051381:Rats; D017386:Sequence Homology, Amino Acid; D020298:U937 Cells
D000911:Antibodies, Monoclonal; D047908:Intracellular Signaling Peptides and Proteins; C093604:LST1 protein, human; D008565:Membrane Proteins; D020033:Protein Isoforms