title: Hydrogen sulfide derived from periadventitial adipose tissue is a vasodilator.
abstract: BACKGROUND
  Recently, periadventitial adipose tissue (PAT) was found to secrete bioactive factors playing an important role in the regulation of vascular tension. Hydrogen sulfide (H(2)S), a novel cardiovascular gasotransmitter, relaxes vessels through the K(ATP) channel in a calcium-dependent and endothelial-independent manner.
  
  
  METHODS
  We first identified the endogenous H(2)S-generated key enzyme cystathionase (CSE) expressed in adipocytes of PAT and H(2)S released from PAT in rats.
  
  
  RESULTS
  The CSE inhibitors DL-propargylglycine and/or beta-cyano-L-alanine largely blocked the vasorelaxing effects on aorta rings (removed adventitia), induced by PAT, the culture medium of PAT or isolated adipocytes from PAT. Phenylephrine, serotonin and angiotensin II inhibited endogenous H(2)S production from the aortic medium but increased its release from PAT. Endogenous H(2)S generated in the aorta and PAT was decreased but the level of CSE protein was increased with the aging of rats. In rats with hypertension induced by abdominal aortic banding, H(2)S generation and CSE protein expression were significantly increased in PAT but not aortic tissues. Transplanting PAT into periadventitia of stenotic aortas ameliorated the elevated arterial blood pressure and decreased angiotensin II level in aorta.
  
  
  CONCLUSIONS
  These results suggested PAT could endogenously generate H(2)S, which might act as an adipocyte-derived relaxing factor and contribute to the pathogenesis of hypertension.
authors: Institute of Cardiovascular Research, First Hospital of Peking University, West District, Beijing, PR China.
authors: Liping
authors: L
authors: Fang
authors: Jing
authors: J
authors: Zhao
authors: Yu
authors: Y
authors: Chen
authors: Tiemin
authors: T
authors: Ma
authors: Guoheng
authors: G
authors: Xu
authors: Chaoshu
authors: C
authors: Tang
authors: Xinmin
authors: X
authors: Liu
authors: Bin
authors: B
authors: Geng
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000502:physiology; D015153:Blotting, Western; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000502:physiology*
chemical_list: D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide