Dual-isotope single-photon emission computed tomography for dopamine and serotonin transporters in normal and Parkinsonian monkey brains.
BACKGROUND
Parkinson's disease (PD) affects both dopaminergic and serotonergic systems. In this study, we simultaneously evaluated dopamine and serotonin transporters in primates using dual-isotope single-photon emission computed tomography (SPECT) imaging and compared the results with traditional single-isotope imaging.


METHODS
Four healthy and one 6-OHDA-induced PD monkeys were used for this study. SPECT was performed over 4 h after individual or simultaneous injection of [(99m)Tc]TRODAT-1 (a dopamine transporter imaging agent) and [(123)I]ADAM (a serotonin transporter imaging agent).


RESULTS
The results showed that the image quality and uptake ratios in different brain regions were comparable between single- and dual-isotope studies. The striatal [(99m)Tc]TRODAT-1 uptake in the PD monkey was markedly lower than that in normal monkeys. The uptake of [(123)I]ADAM in the midbrain of the PD monkey was comparable to that in the normal monkeys, but there were decreased uptakes in the thalamus and striatum of the PD monkey.


CONCLUSIONS
Our results suggest that dual-isotope SPECT using [(99m)Tc]TRODAT-1 and [(123)I]ADAM can simultaneously evaluate changes in dopaminergic and serotonergic systems in a PD model.
Li
I-Hsun
IH
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan.
Huang
Wen-Sheng
WS
Yeh
Chin-Bin
CB
Liao
Mei-Hsiu
MH
Chen
Chia-Chieh
CC
Shen
Lie-Hang
LH
Liu
Jiang-Chuan
JC
Ma
Kuo-Hsing
KH
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism; D002928:Cinanserin / Q000031:analogs & derivatives* / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism*; D005240:Feasibility Studies; D008251:Macaca; D015609:Organotechnetium Compounds / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology; D010300:Parkinson Disease / Q000000981:diagnostic imaging* / Q000209:etiology / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D013997:Time Factors; D015899:Tomography, Emission-Computed, Single-Photon; D014326:Tropanes / Q000378:metabolism
C413491:2-((2-((dimethylamino)methyl)phenyl)thio)-5-iodophenylamine; D050483:Dopamine Plasma Membrane Transport Proteins; D015609:Organotechnetium Compounds; D050486:Serotonin Plasma Membrane Transport Proteins; D014326:Tropanes; C104196:technetium Tc 99m TRODAT-1; D016627:Oxidopamine; D002928:Cinanserin