title: MOV10 as a novel telomerase-associated protein.
abstract: A novel telomerase-associated protein was isolated from porcine testis. The 115-kDa protein, purified with telomerase activity, was molecular cloned using human cDNA library, and identified as MOV10. The expression levels of both MOV10 mRNA and MOV10 protein in cancer cells were 2-3 times higher than that of the normal cells, and MOV10 mRNA was highly expressed in human testis and ovary. The anti-MOV10 antibody precipitated the telomerase activity from cancer cell extracts, and inhibited the telomerase activity in vitro. Sf9-expressed MOV10 protein bound to G-rich strand of both single- and double-stranded telomere-sequenced DNA, but not to single C-rich strand. ChIP assay showed the binding of MOV10 to telomere region in vivo. These data suggest that MOV10 is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.
authors: Department of Biology, Faculty of Science, Ochanomizu University, Tokyo 112-8610, Japan.
authors: Mariko
authors: M
authors: Nakano
authors: Yasutaka
authors: Y
authors: Kakiuchi
authors: Yukiko
authors: Y
authors: Shimada
authors: Mika
authors: M
authors: Ohyama
authors: Yasuko
authors: Y
authors: Ogiwara
authors: Narie
authors: N
authors: Sasaki-Higashiyama
authors: Naoko
authors: N
authors: Yano
authors: Fumi
authors: F
authors: Ikeda
authors: Ei
authors: E
authors: Yamada
authors: Akihiro
authors: A
authors: Iwamatsu
authors: Kazuo
authors: K
authors: Kobayashi
authors: Kiyoto
authors: K
authors: Nishiyama
authors: Sohsuke
authors: S
authors: Ichikawa
authors: Kazuhiko
authors: K
authors: Kaji
authors: Toshinori
authors: T
authors: Ide
authors: Hiromu
authors: H
authors: Murofushi
authors: Kimiko
authors: K
authors: Murakami-Murofushi
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D008297:Male; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000201:enzymology*; D013737:Testis / Q000201:enzymology
chemical_list: D004247:DNA; C530476:Mov10 protein, human; D019098:Telomerase; D020365:RNA Helicases