Cell-selective metabolic labeling of proteins.
Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.
Ngo
John T
JT
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.
Champion
Julie A
JA
Mahdavi
Alborz
A
Tanrikulu
I Caglar
IC
Beatty
Kimberly E
KE
Connor
Rebecca E
RE
Yoo
Tae Hyeon
TH
Dieterich
Daniela C
DC
Schuman
Erin M
EM
Tirrell
David A
DA
D000345:Affinity Labels / Q000378:metabolism*; D000409:Alanine / Q000031:analogs & derivatives / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000382:microbiology; D008718:Methionine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009646:Norleucine / Q000031:analogs & derivatives / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*
D000345:Affinity Labels; D011506:Proteins; D009646:Norleucine; D000604:Amino Acyl-tRNA Synthetases; D008718:Methionine-tRNA Ligase; D000409:Alanine