title: Side-chain structure of cell surface polysaccharide, mannan, affects hypocholesterolemic activity of yeast.
abstract: We previously reported that Kluyveromyces marxianus YIT 8292 exhibited more potent hypocholesterolemic activity than other yeasts containing Saccharomyces cerevisiae . To clarify the reason for the higher hypocholesterolemic activity, we examined the side-chain structure of cell surface polysaccharide, mannan, of K. marxianus YIT 8292. The result shows that K. marxianus YIT 8292 had shorter alpha-(1,2)-linked oligomannosyl side chains and lower phosphate content in mannan than S. cerevisiae. The association between its structural features and hypocholesterolemic activity was investigated by comparing the hypocholesterolemic activities of S. cerevisiae mannan mutants in rats fed a high-cholesterol diet. S. cerevisiae mnn5 mutant with deficiencies in the phosphorylation and elongation of mannan side chains showed higher hypocholesterolemic activity than the wild-type strain. These results show that the side-chain length and phosphate contents of mannan affect hypocholesterolemic activity.
authors: Yakult Central Institute for Microbiological Research, Kunitachi-shi, Tokyo 186-8650, Japan. yasuto-yoshida@yakult.co.jp
authors: Yasuto
authors: Y
authors: Yoshida
authors: Eiichiro
authors: E
authors: Naito
authors: Harumi
authors: H
authors: Mizukoshi
authors: Yoko
authors: Y
authors: Watanabe
authors: Kazumasa
authors: K
authors: Kimura
authors: Wakae
authors: W
authors: Yokoi
authors: Tadashi
authors: T
authors: Sato
authors: Takekazu
authors: T
authors: Okumura
authors: Masahiko
authors: M
authors: Ito
authors: Haruji
authors: H
authors: Sawada
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents; D002473:Cell Wall / Q000737:chemistry*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D007716:Kluyveromyces / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D008055:Lipids / Q000097:blood; D008297:Male; D008351:Mannans / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D010710:Phosphates / Q000032:analysis; D019936:Probiotics / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D013329:Structure-Activity Relationship
chemical_list: D000924:Anticholesteremic Agents; D002791:Cholesterol, Dietary; D008055:Lipids; D008351:Mannans; D010710:Phosphates