title: Anion exchange liquid chromatography for the determination of nucleotides in baby and/or functional foods.
abstract: A sensitive, selective and solvent-free procedure is proposed for the rapid determination of monophosphate nucleotides (cytidine 5'-monophosphate, uridine 5'-monophosphate, adenosine 5'-monophosphate, and guanosine 5'-monophosphate) in baby foods. The method is based on the deproteinization of foods and direct analysis by anion exchange liquid chromatography (LC). Nucleotides were separated on an SAX anion exchange column with isocratic elution using 0.01 M dihydrogenphosphate buffer (pH 3.5) as mobile phase at a flow-rate of 1 mL min(-1) and detected by diode-array detection (DAD). The LC method rapidly separated the nucleotides (9 min) and was validated for linearity, detection and quantitation limits, selectivity, accuracy, and precision. The recoveries obtained for spiked samples were satisfactory for all the analytes. The proposed procedure allowed the only authorized nucleotides in infant formulas to be determined and was successfully applied to the analysis of different baby and/or functional food samples, including infant formulas, fermented milk, cereals, and purees.
authors: Departamento de Quimica Analitica, Facultad de Quimica, Universidad de Murcia, Murcia, Spain.
authors: Pilar
authors: P
authors: Viñas
authors: Natalia
authors: N
authors: Campillo
authors: Ignacio
authors: I
authors: López-García
authors: Sara
authors: S
authors: Martínez-López
authors: Isabel
authors: I
authors: Vasallo
authors: Manuel
authors: M
authors: Hernández-Córdoba
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D055951:Functional Food / Q000032:analysis*; D006801:Humans; D007225:Infant Food / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*
chemical_list: D009711:Nucleotides