title: Digital transcriptome profiling using selective hexamer priming for cDNA synthesis.
abstract: We developed a procedure for the preparation of whole transcriptome cDNA libraries depleted of ribosomal RNA from only 1 microg of total RNA. The method relies on a collection of short, computationally selected oligonucleotides, called 'not-so-random' (NSR) primers, to obtain full-length, strand-specific representation of nonribosomal RNA transcripts. In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing.
authors: Department of Molecular Informatics, Rosetta Inpharmatics LLC, a wholly owned subsidiary of Merck and Co., Inc., Seattle, Washington, USA. christopher_armour@merck.com
authors: Christopher D
authors: CD
authors: Armour
authors: John C
authors: JC
authors: Castle
authors: Ronghua
authors: R
authors: Chen
authors: Tomas
authors: T
authors: Babak
authors: Patrick
authors: P
authors: Loerch
authors: Stuart
authors: S
authors: Jackson
authors: Jyoti K
authors: JK
authors: Shah
authors: John
authors: J
authors: Dey
authors: Carol A
authors: CA
authors: Rohl
authors: Jason M
authors: JM
authors: Johnson
authors: Christopher K
authors: CK
authors: Raymond
mesh_terms: D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D006801:Humans; D012313:RNA / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; C034477:RNA primers; D012313:RNA