Differential fluorescent staining of Listeria monocytogenes and a whey food soil for quantitative analysis of surface hygiene.
The accurate monitoring of surface cleanliness in terms of bacterial contamination is usually carried out using methods such as plate counts or replica plating. However these methods take at least eighteen hours to obtain results and do not determine the presence or amount of residual organic material on a surface, which may interfere with cleaning and disinfection. This work describes the application of fluorescent stains to cells (Listeria monocytogenes) and food soil (solubilized whey) to optimize a dual staining method that can be used in the quantitative analysis of surface cleanability. Seven different stains were tested at a range of concentrations (0.3%-0.001 mg/ml) and application methods. The best stain combination for differential staining of L. monocytogenes and whey food soil was 0.1 mg/ml rhodamine B with 0.1 g/ml DAPI. Differential staining of the cells and soil occurred regardless of the application method. This method has been successfully used to demonstrate the hygienic status of surfaces in an industrial situation. This novel work enables quantitative assessment of soils and cells on surfaces.
Whitehead
Kathryn A
KA
School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Chester St, Manchester M1 5GD, UK. K.A.Whitehead@mmu.ac.uk
Benson
Paul
P
Verran
Joanna
J
D015169:Colony Count, Microbial; D004783:Environmental Microbiology; D004866:Equipment Contamination; D005516:Food Microbiology; D005525:Food-Processing Industry / Q000592:standards*; D006920:Hygiene; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008856:Microscopy, Fluorescence; D008894:Milk Proteins / Q000302:isolation & purification*; D013193:Stainless Steel; D013499:Surface Properties; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins; D013193:Stainless Steel