title: Estimation of the effective orientation of the SHG source in primary cortical neurons.
abstract: In this paper we provide, for the first time to our knowledge, the effective orientation of the SHG source in cultured cortical neuronal processes in vitro. This is done by the use of the polarization sensitive second harmonic generation (PSHG) imaging microscopy technique. By performing a pixel-level resolution analysis we found that the SHG dipole source has a distribution of angles centered at thetae =33.96 degrees , with a bandwidth of Deltathetae = 12.85 degrees . This orientation can be related with the molecular geometry of the tubulin heterodimmer contained in microtubules.
authors: ICFO-Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860 Castelldefels, Barcelona, Spain.
authors: Sotiris
authors: S
authors: Psilodimitrakopoulos
authors: Valerie
authors: V
authors: Petegnief
authors: Guadalupe
authors: G
authors: Soria
authors: Ivan
authors: I
authors: Amat-Roldan
authors: David
authors: D
authors: Artigas
authors: Anna M
authors: AM
authors: Planas
authors: Pablo
authors: P
authors: Loza-Alvarez
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology*; D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008962:Models, Theoretical; D009474:Neurons / Q000166:cytology*; D055096:Optical Devices; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation