title: Age-dependent differences in galanin-dependent colonic fluid secretion after infection with Salmonella typhimurium.
abstract: Epithelial cells lining the colon do not normally express galanin type 1 receptors (Gal1Rs). However, subsequent to infection with enteric pathogens such as Salmonella typhimurium, the Gal1R is rapidly upregulated in colonocytes where it contributes to the excess fluid production associated with diarrhoea. Humans infected with non-typhoid Salmonella respond differently according to age: infants develop diarrhoea but not bacteraemia and survive, while the elderly become bacteraemic and die. Thus the aim of this study was to determine if age-related differences exist in response to S typhimurium infection in mice, and whether these differences are due to altered Gal1R expression. Wild-type C57BL/6J mice that were 2 and 15 months old, as well as 2-month-old Gal1R knockout mice, were infected by gavage. Young wild-type mice expressed Gal1R in response to infection, had increased colonic fluid secretion, low rates of bacteraemia and survived. In contrast, 15-month-old wild-type mice expressed fewer Gal1Rs in response to infection, had attenuated increases in colonic fluid secretion, high rates of bacteraemia and died. A similar profile was noted in 2-month-old Gal1R knockout mice. Addition of polyethylene glycol to the drinking water of 15-month-old wild-type mice increased colonic fluid secretion and reduced rates of bacteraemia to those observed in 2-month-old wild-type mice and eliminated fatalities. The difference in response to S typhimurium infection with age may be due, at least in part, to decreased Gal1R expression and decreased amounts of colonic fluid secretion.
authors: Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors: K
authors: K
authors: Matkowskyj
authors: S V
authors: SV
authors: Royan
authors: A
authors: A
authors: Blunier
authors: G
authors: G
authors: Hecht
authors: M
authors: M
authors: Rao
authors: R V
authors: RV
authors: Benya
mesh_terms: D000367:Age Factors; D000818:Animals; D016470:Bacteremia; D003106:Colon; D003967:Diarrhea / Q000378:metabolism; D017615:Enteric Nervous System / Q000378:metabolism; D019004:Galanin / Q000032:analysis / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000276:immunology; D007419:Intestinal Secretions / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism; D011092:Polyethylene Glycols / Q000627:therapeutic use; D044091:Receptor, Galanin, Type 1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality; D012486:Salmonella typhimurium; D013047:Specific Pathogen-Free Organisms; D013501:Surface-Active Agents / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D044091:Receptor, Galanin, Type 1; D013501:Surface-Active Agents; D014409:Tumor Necrosis Factor-alpha; D011092:Polyethylene Glycols; D019004:Galanin