Immune response of horses to vaccination with the recombinant Hc domain of botulinum neurotoxin types C and D.
Botulinum neurotoxins, predominantly serotypes C and D, cause equine botulism through forage poisoning. The C-terminal part of the heavy chain of botulinum neurotoxin types C and D (HcBoNT/C and D) was expressed in Escherichia coli and evaluated as a recombinant mono- and bivalent vaccine in twelve horses in comparison to a commercially available toxoid vaccine. A three-dose subcutaneous immunization of adult horses elicited robust serum antibody response in an ELISA using the immunogen as a capture antigen. Immune sera showed dose-dependent high potency in neutralizing specifically the active BoNT/C and D in the mouse protection assay. The aluminium hydroxide based mono- and bivalent recombinant HcBoNT/C and D vaccines were characterized by good compatibility and the ability to elicit protective antibody titers similar or superior to the commercially available toxoid vaccine.
Stahl
Christina
C
Equine Clinic, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Berne, Länggassstrasse 124, CH-3012 Berne, Switzerland.
Unger
Lucia
L
Mazuet
Christelle
C
Popoff
Michel
M
Straub
Reto
R
Frey
Joachim
J
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000992:Antitoxins / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001905:Botulinum Toxins / Q000235:genetics / Q000276:immunology*; D001906:Botulism / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D006736:Horses; D007117:Immunization, Secondary / Q000379:methods; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D017434:Protein Structure, Tertiary; D014614:Vaccines, Synthetic / Q000008:administration & dosage
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000992:Antitoxins; D001428:Bacterial Vaccines; D014614:Vaccines, Synthetic; C096325:botulinum toxin type D; D000536:Aluminum Hydroxide; D001905:Botulinum Toxins; C096324:botulinum toxin type C