title: DNA strands from denatured duplexes are translocated through engineered protein nanopores at alkaline pH.
abstract: Nanopores are under development for the detection of a variety of analytes and the investigation of chemical reactions at the single molecule level. In particular, the analysis of nucleic acid molecules is under intense investigation, including the development of systems for rapid, low-cost DNA sequencing. Here, we show that DNA can be translocated through an engineered alphaHL protein pore at pH 11.7, a value at which dsDNA is denatured. Therefore, the alphaHL pore is sufficiently stable to entertain the possibility of direct nanopore sequencing of genomic dsDNA samples, which are more readily obtained and handled than ssDNA.
authors: Department of Chemistry, University of Oxford, Oxford, OX1 3TA, United Kingdom.
authors: Giovanni
authors: G
authors: Maglia
authors: Marsiyana
authors: M
authors: Henricus
authors: Romain
authors: R
authors: Wyss
authors: Qiuhong
authors: Q
authors: Li
authors: Stephen
authors: S
authors: Cheley
authors: Hagan
authors: H
authors: Bayley
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D006460:Hemolysin Proteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology; D009691:Nucleic Acid Denaturation; D011506:Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D006460:Hemolysin Proteins; D011506:Proteins; D004247:DNA