Salsuginibacillus halophilus sp. nov., a halophilic bacterium isolated from a soda lake.
A Gram-stain-positive, rod-shaped, endospore-forming, halophilic, alkalitolerant bacterium, designated halo-1(T), was isolated from sediment of Xiarinaoer soda lake, located in the Inner Mongolia Autonomous Region of China. Strain halo-1(T) grew in the presence of 9-30 % (w/v) NaCl (optimum 19 %) and at pH 5-10 (optimum pH 9). The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids of the isolate were anteiso-C(15 : 0) (58.35 %), anteiso-C(17 : 0) (12.89 %) and C(16 : 0) (6.52 %). The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and a phospholipid of unknown structure. The DNA G+C content of the strain was 46.4 mol%. On the basis of 16S rRNA gene sequence similarity, strain halo-1(T) showed the highest similarity (93.9 %) to Salsuginibacillus kocurii CH9d(T). Strain halo-1(T) could be clearly differentiated from its closest phylogenetic relative on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain halo-1(T) represents a novel species, for which the name Salsuginibacillus halophilus sp. nov. is proposed, with the type strain halo-1(T) (=CGMCC 1.7653(T) =NBRC 104934(T)).
Cao
Shu-Juan
SJ
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Qu
Jian-Hang
JH
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Yuan
Hong-Li
HL
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Li
Bao-Zhen
BZ
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
D004777:Environment; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D016987:Gram-Positive Rods / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010858:Pigmentation; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012965:Sodium Chloride / Q000378:metabolism*; D014871:Water Microbiology
D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride