Salinarimonas rosea gen. nov., sp. nov., a new member of the alpha-2 subgroup of the Proteobacteria.
A Gram-negative, rod-shaped, facultatively anaerobic, halotolerant bacterial strain, designated YIM YD3(T), was isolated from a salt mine in Yunnan, south-west China. The taxonomy of strain YIM YD3(T) was investigated by a polyphasic approach. Strain YIM YD3(T) was motile, formed pink colonies and was positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and two unknown phospholipids. The major fatty acids (>10 % of total fatty acids) were C(18 : 1)omega7c, C(18 : 1)omega9c, C(16 : 0) and C(19 : 0) cyclo omega8c. The DNA G+C content was 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate formed a distinct line within a clade containing the genera Balneimonas, Bosea, Chelatococcus and Microvirga in the order Rhizobiales, with highest levels of 16S RNA gene sequence similarity to the type strain of Balneimonas flocculans (93.5 %). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain YIM YD3(T) represents a novel species in a new genus, for which the name Salinarimonas rosea gen. nov., sp. nov. is proposed, with strain YIM YD3(T) (=KCTC 22346(T)=CCTCC AA208038(T)) as the type strain.
Liu
Ji-Hui
JH
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Wang
Yong-Xia
YX
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Zhang
Xiao-Xia
XX
Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100080, PR China.
Wang
Zhi-Gang
ZG
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Chen
Yi-Guang
YG
College of Bio-resources and Environmental Science, Jishou University, Jishou, Hunan 416000, PR China.|Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Wen
Meng-Liang
ML
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Xu
Li-Hua
LH
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Peng
Qian
Q
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Cui
Xiao-Long
XL
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008906:Mining; D008969:Molecular Sequence Data; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S