title: CelluSpots: a reproducible means of making peptide arrays for the determination of SH2 domain binding specificity.
abstract: Peptide arrays differ from conventional peptide synthesis in that hundreds upon thousands of peptides are synthesized and presented on a planar surface at a time. While direct synthesis of peptide arrays on a functionalized surface is feasible, reprinting of pre-made peptides offers flexibility and reproducibility and drastically reduces cost when multiple copies of the same or related peptide arrays are needed. CelluSpot, a method developed by Intavis, opens a new route in peptide array synthesis and printing and overcomes certain limitations of the SPOT membrane. This technique was used to produce hundreds of phosphotyrosine-oriented peptide array libraries for determining the specificity of the Src homology 2 (SH2) domain.
authors: Department of Biochemistry and the Siebens-Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.
authors: Chenggang
authors: C
authors: Wu
authors: Shawn S-C
authors: SS
authors: Li
mesh_terms: D000818:Animals; D002482:Cellulose / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis / Q000493:pharmacokinetics; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D013379:Substrate Specificity; D018909:src Homology Domains
chemical_list: D008567:Membranes, Artificial; D010455:Peptides; D002482:Cellulose