title: Characterization of mesenchymal stem cells (MSCs) from human fetal lung: potential differentiation of germ cells.
abstract: Pluripotent mesenchymal stem-like cell lines were established from lungs of 3-4 months old aborted fetus. The cells present the high ex vivo expansion potential of MSC, a typical fibroblast-like morphology and proliferate up to 15 passages without displaying clear changes in morphology. Immunological localization and flow cytometry analyses showed that these cells are positive for OCT4, c-Kit, CD11, CD29, CD44, telomerase, CD106, CD105, CD166, and SSEA1, weakly expression or negative for SSEA1, SSEA3, SSEA4, CD34, CD105 and CD106. These cells can give rise to the adipogenic as evidenced by accumulation of lipid-rich vacuoles within cells identified by Oil-red O when they were induced with 0.5 mM isobutylmethylxanthine, 200 microM indomethacin, 10(-6)M dexamethasone, and 10 microg/ml of insulin in high-glucose DMEM. Osteogenic lineage cells were generated in 0.1 microM dexamethasone, 50 microg/ml ascorbic acid, 10 mM beta-glycerophosphate, which are shaped as the osteoblastic morphology, expression of alkaline phosphatase (AP), and the formation of a mineralized extracellular matrix identified by Alizarin Red staining. Neural cells are observed when the cultures were induced with 2-mercapometal, which are positive for nestin, NF-100, MBP and GFAP. Additionally, embryoid bodies (EBs) and sperm like cells are obtained in vitro differentiation of these lung MSCs induced with 10(-5)M retinoic acid (RA). These results demonstrated that these MSCs are pluripotent and may provide an in vitro model to study germ-cell formation and also as a potential source of sperms for male infertility.
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest, A&F University, Yangling, Shaanxi, China. jinlianhua@nwsuaf.edu.cn
authors: Jinlian
authors: J
authors: Hua
authors: Haisheng
authors: H
authors: Yu
authors: Wuzi
authors: W
authors: Dong
authors: Chunrong
authors: C
authors: Yang
authors: Zhimin
authors: Z
authors: Gao
authors: Anmin
authors: A
authors: Lei
authors: Yadong
authors: Y
authors: Sun
authors: Shaohui
authors: S
authors: Pan
authors: Yuanyuan
authors: Y
authors: Wu
authors: Zhongying
authors: Z
authors: Dou
mesh_terms: D037881:Aborted Fetus / Q000166:cytology* / Q000378:metabolism; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells
chemical_list: D000943:Antigens, Differentiation