title: Dynamic progesterone responses to simulation of a natural pulse of a metabolite of prostaglandin F(2alpha) in heifers.
abstract: The effects of prostaglandin F(2alpha) (PGF) on circulating progesterone concentration were studied in four groups (n=4) of Holstein heifers 9d after ovulation. The progesterone response to simulation of a pulse of 13,14-dihydro-15-keto-PGF (PGFM) by a 2-h intrauterine (IU) infusion of 0.5mg of PGF was compared with the response to a PGF-bolus IU injection of 4mg. The beginning of infusion and time of injection were designated Minute 0. Progesterone concentration did not change significantly between Minute 0 and Hour 48 in control or IU vehicle-treated groups. In the PGF-bolus group, progesterone concentration increased (P<0.05) between Minutes 0 and 10 and then decreased. In the PGF-infusion group, simulation of a PGFM pulse was not associated with an initial transient increase in progesterone. The first significant decrease (P<0.05) in progesterone began at Minute 20 and continued until Hour 1. The progesterone concentration then began to rebound (P<0.05) at Hour 1 and peaked at Hour 3 at almost the same concentration as at the start of PGF infusion. The progesterone again decreased after Hour 3 and increased again between Hours 24 and 48. In summary: (1) an initial transient increase in progesterone was not detected in association with an individual simulated pulse of PGF, indicating that the frequently reported pronounced transient increase after a bolus luteolytic dose of PGF is a nonphysiological response and (2) simulation of a PGFM pulse resulted in a distinct transient rebound in progesterone beginning at Hour 1 of the PGF infusion.
authors: Eutheria Foundation, Cross Plains, WI 53528, USA.
authors: H K
authors: HK
authors: Shrestha
authors: M A
authors: MA
authors: Beg
authors: M A R
authors: MA
authors: Siddiqui
authors: O J
authors: OJ
authors: Ginther
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood*; D015237:Dinoprost / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D007700:Kinetics; D010060:Ovulation; D011374:Progesterone / Q000097:blood*; D014599:Uterus / Q000187:drug effects
chemical_list: C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D015237:Dinoprost