Long-lasting infiltration anaesthesia by lidocaine-loaded biodegradable nanoparticles in hydrogel in rats.
BACKGROUND
Infiltration of a long-lasting anaesthetic is helpful during the post-operative period. The recently developed local drug delivery system, biodegradable nanoparticles in a thermo-sensitive hydrogel (nanogel system), may possibly provide an extended duration of drugs. Therefore, we evaluated whether prolonged infiltration anaesthesia could be achieved by loading lidocaine into this delivery system.


METHODS
Thirty male rats were randomized into five groups of six rats each: saline; 2% hydrochloride lidocaine solution; lidocaine-loaded nanogel system and its compositing formulations, namely lido-nano gel; lido-nano; and lidogel. Durations of local anaesthesia with subcutaneously injected agents were measured by tail flick latency tests in a randomized, blind fashion.


RESULTS
Lido-nano gel produced effective anaesthesia for 360+/-113 min, compared with 150+/-33 min by lidogel, 180+/-37 min by lido-nano, and 110+/-45 min by lidocaine solution (P<0.001, means+/-SD), and elicited complete sensory blockade for 300+/-114 min, compared with 75+/-37 min by lidogel, 105+/-53 min by lido-nano, and 60+/-33 min by lidocaine solution (P<0.001, means+/-SD) without severe skin/systemic toxicity.


CONCLUSIONS
Lidocaine-loaded biodegradable nanoparticles in hydrogel produced prolonged infiltration anaesthesia in rats without severe toxicity, indicating a possible way to develop long-lasting local anaesthetics.
Yin
Q-Q
QQ
Laboratory of Anaesthesiology and Critical Care Medicine, Department of Anaesthesiology, West China Hospital, Sichuan University, Chengdu, China.
Wu
L
L
Gou
M-L
ML
Qian
Z-Y
ZY
Zhang
W-S
WS
Liu
J
J
D000772:Anesthesia, Local / Q000009:adverse effects; D000779:Anesthetics, Local / Q000009:adverse effects; D000818:Animals; D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D003877:Dermatitis, Contact / Q000473:pathology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007202:Indicators and Reagents; D008012:Lidocaine / Q000009:adverse effects; D008297:Male; D053758:Nanoparticles; D010147:Pain Measurement / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D000779:Anesthetics, Local; D003692:Delayed-Action Preparations; D007202:Indicators and Reagents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008012:Lidocaine