A rapidly resorbable hemostatic biomaterial based on dihydroxyacetone.
We have developed a rapid acting, rapidly resorbable, non-toxic, topical hemostatic agent comprised of a PEGylated, polymerized sequence of dihydroxyacetone (MPEG-pDHA) that is highly effective in vivo. Twenty-eight Sprague-Dawley rats underwent left lateral hepatectomy. To the cut edge of the liver, rats received MPEG-pDHA (50 mg), normal saline (0.5 mL), or Instat (50 mg), a commercially available hemostatic compound. Bleeding time and total blood loss were quantified. Coagulation studies and scanning electron microscopy were performed on phlebotomized blood combined with MPEG-pDHA. Rats treated with MPEG-pDHA had significantly decreased bleeding time (97 s) and total blood loss (1.35 g) compared to normal saline (464 s and 3.83 g, p < 0.05 for each), and a significantly shorter bleeding time compared to Instat (165 s, p < 0.05). Histology confirmed that all MPEG-pDHA was metabolized within 3 weeks. The addition of MPEG-pDHA to whole blood did not significantly affect prothrombin time (12.0 s vs. 13.2 s, p = 0.130), partial thromboplastin time (27.0 s vs. 21.8 s, p = 0.118), or thrombin clotting time. MPEG-pDHA is an effective and rapidly resorbable hemostatic agent that may find broad hemostatic application in a wide range of surgical procedures.
Henderson
Peter W
PW
Division of Plastic and Reconstructive Surgery, Laboratory for Bioregenerative Medicine and Surgery, Weill Cornell Medical College, New York, New York, USA.
Kadouch
Daniel J M
DJ
Singh
Sunil P
SP
Zawaneh
Peter N
PN
Weiser
Jennifer
J
Yazdi
Sara
S
Weinstein
Andrew
A
Krotscheck
Ursula
U
Wechsler
Bennett
B
Putnam
David
D
Spector
Jason A
JA
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001780:Blood Coagulation Tests; D004098:Dihydroxyacetone / Q000737:chemistry / Q000378:metabolism; D006490:Hemostatics / Q000737:chemistry / Q000378:metabolism; D006498:Hepatectomy; D008099:Liver / Q000293:injuries; D008297:Male; D008422:Materials Testing; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D001672:Biocompatible Materials; D006490:Hemostatics; D011092:Polyethylene Glycols; D004098:Dihydroxyacetone