Constituents of Tinospora sinensis and their antileishmanial activity against Leishmania donovani.
Two new compounds 4-methyl-heptadec-6-enoic acid ethyl ester (2) and 3-hydroxy-2,9,11-trimethoxy-5,6-dihydro isoquino[3,2-a]isoquinolinylium (7) were isolated from an ethanolic extract of the stems of Tinospora sinensis, along with six known compounds (1, 3-6 and 8). The structures of new compounds were established on the basis of detailed spectroscopic studies. Compound 7 exhibited the highest in vitro antileishmanial activity against Leishmania donovani promastigotes and intracellular amastigotes, whereas compounds 2, 4, 5 and 6 demonstrated moderate activity. Other compounds were found to be inactive.
Maurya
Rakesh
R
Division of Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow, India. mauryarakesh@rediffmail.com
Gupta
Prasoon
P
Chand
Kailash
K
Kumar
Manmeet
M
Dixit
Preety
P
Singh
Nasib
N
Dube
Anuradha
A
D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007893:Leishmania donovani / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031610:Tinospora / Q000737:chemistry*
D000981:Antiprotozoal Agents; D010936:Plant Extracts