Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina).
A non-pigmented, motile, Gram-negative bacterium, strain Z 271(T), was isolated from the surface of leaves of the seagrass Zostera marina which was collected in Troitza Bay (Sea of Japan, Pacific Ocean). The new isolate grew between 5 degrees C and 28 degrees C and was slightly halophilic, tolerating environments containing up to 5 % (w/v) NaCl. Strain Z 271(T) was able to degrade Tweens 20, 40 and 80 and partially degrade gelatin, but was unable to degrade casein. Phosphatidylethanolamine (36.9 %) and phosphatidylglycerol (63.1 %) were the predominant phospholipids. The major fatty acids included C(18 : 1)omega7c (43.7 %), C(16 : 1)omega7c (31.1 %) and C(16 : 0) (16.8 %). The main respiratory quinone was Q-8. The DNA-DNA relatedness value of strain Z 271(T) with Granulosicoccus antarcticus IMCC3135(T) was 35 %. The G+C content of the DNA of strain Z 271(T) was 60.2 mol%. On the basis of phenotypic and genotypic characteristics and 16S rRNA gene sequence analysis, strain Z 271(T) represents a novel species of the genus Granulosicoccus for which the name Granulosicoccus coccoides sp. nov. is proposed. The type strain is Z 271(T) (=KMM 6014(T)=CIP 109923(T)).
Kurilenko
Valerie V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
Christen
Richard
R
Université de Nice Sophia-Antipolis and Centre National de la Recherche Scientifique (CNRS) UMR 6543, Centre de Biochimie, Parc Valrose, F 06142 Nice, France.
Zhukova
Natalia V
NV
Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041 Vladivostok, Russian Federation.
Kalinovskaya
Nataliya I
NI
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
Mikhailov
Valery V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
Crawford
Russell J
RJ
Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.
Ivanova
Elena P
EP
Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D035962:Zosteraceae / Q000382:microbiology*
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S