Identification of a Candida parapsilosis strain producing extracellular serine peptidase with keratinolytic activity.
A yeast strain isolated from feather waste from a chicken processing plant was identified as Candida parapsilosis by biochemical tests and morphological studies. The yeast was able to grow in phosphate-buffered saline supplemented with 1% native feather as the sole carbon and nitrogen source. A keratin substrate was obtained from the feathers by dimethylsulphoxide extraction. A 20-fold concentrated culture supernatant from Candida parapsilosis grown on feathers was analysed by SDS-PAGE electrophoresis containing either 1% gelatin or 1% keratin as copolymerised substrates. The presence of a single band with an approximate molecular mass of 60 kDa with gelatinolytic and keratinolytic activities was observed. This proteolytic activity was fully inhibited by phenylmethylsulphonyl fluoride. These results suggest that the extracellular enzyme belongs to the serine peptidase class. This is the first report of an extracellular serine peptidase produced by C. parapsilosis with keratinolytic activity. The role of this enzyme in yeast-host interactions is discussed.
Vermelho
Alane Beatriz
AB
Laboratório Protease de Microrganismos, Departamento de Microbiologia Geral, Instituto de Microbiologia Prof Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. abvermelho@micro.ufrj.br
Mazotto
Ana Maria
AM
de Melo
Ana Cristina Nogueira
AC
Vieira
Flávia Helena Cardoso
FH
Duarte
Thalita Rodrigues
TR
Macrae
Andrew
A
Nishikawa
Marília Martins
MM
da Silva Bon
Elba Pinto
EP
D000818:Animals; D002175:Candida / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D002645:Chickens; D005110:Extracellular Space / Q000201:enzymology*; D005241:Feathers / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D007220:Industrial Waste; D007633:Keratins / Q000378:metabolism; D008970:Molecular Weight; D014009:Onychomycosis / Q000201:enzymology / Q000382:microbiology; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D057057:Serine Proteases / Q000737:chemistry / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013379:Substrate Specificity
D007220:Industrial Waste; D015842:Serine Proteinase Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D007633:Keratins; D005780:Gelatin; D010447:Peptide Hydrolases; D057057:Serine Proteases; C035855:keratinase