The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity.
The sensitive to lysis D (SlyD) protein from Escherichia coli is related to the FK506-binding protein family, and it harbours both peptidyl-prolyl cis-trans isomerase (PPIase) and chaperone-like activity, preventing aggregation and promoting the correct folding of other proteins. Whereas a functional role of SlyD as a protein-folding catalyst in vivo remains unclear, SlyD has been shown to be an essential component for [Ni-Fe]-hydrogenase metallocentre assembly in bacteria. Interestingly, the isomerase activity of SlyD is uniquely modulated by nickel ions, which possibly regulate its functions in response to external stimuli. In this work, we investigated the solution structure of SlyD and its interaction with nickel ions, enabling us to gain insights into the molecular mechanism of this regulation. We have revealed that the PPIase module of SlyD contains an additional C-terminal alpha-helix packed against the catalytic site of the domain; unexpectedly, our results show that the interaction of SlyD with nickel ions entails participation of the novel structural features of the PPIase domain, eliciting structural alterations of the catalytic pocket. We suggest that such conformational rearrangements upon metal binding underlie the ability of nickel ions to regulate the isomerase activity of SlyD.
Martino
Luigi
L
Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.
He
Yangzi
Y
Hands-Taylor
Katherine L D
KL
Valentine
Elizabeth R
ER
Kelly
Geoff
G
Giancola
Concetta
C
Conte
Maria R
MR
D002151:Calorimetry; D020134:Catalytic Domain; D029968:Escherichia coli Proteins / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009532:Nickel / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012996:Solutions; D013816:Thermodynamics
D029968:Escherichia coli Proteins; C085606:SlyD protein, E coli; D012996:Solutions; D009532:Nickel; D019696:Peptidylprolyl Isomerase