title: Dimerization of hepatitis E virus capsid protein E2s domain is essential for virus-host interaction.
abstract: Hepatitis E virus (HEV), a non-enveloped, positive-stranded RNA virus, is transmitted in a faecal-oral manner, and causes acute liver diseases in humans. The HEV capsid is made up of capsomeres consisting of homodimers of a single structural capsid protein forming the virus shell. These dimers are believed to protrude from the viral surface and to interact with host cells to initiate infection. To date, no structural information is available for any of the HEV proteins. Here, we report for the first time the crystal structure of the HEV capsid protein domain E2s, a protruding domain, together with functional studies to illustrate that this domain forms a tight homodimer and that this dimerization is essential for HEV-host interactions. In addition, we also show that the neutralizing antibody recognition site of HEV is located on the E2s domain. Our study will aid in the development of vaccines and, subsequently, specific inhibitors for HEV.
authors: National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Sciences, Xiamen University, Xiamen, China.
authors: Shaowei
authors: S
authors: Li
authors: Xuhua
authors: X
authors: Tang
authors: J
authors: J
authors: Seetharaman
authors: Chunyan
authors: C
authors: Yang
authors: Ying
authors: Y
authors: Gu
authors: Jun
authors: J
authors: Zhang
authors: Hailian
authors: H
authors: Du
authors: J Wai Kuo
authors: JW
authors: Shih
authors: Choy-Leong
authors: CL
authors: Hew
authors: J
authors: J
authors: Sivaraman
authors: Ningshao
authors: N
authors: Xia
mesh_terms: D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D016752:Hepatitis E virus / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006790:Host-Parasite Interactions / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins