title: Tistlia consotensis gen. nov., sp. nov., an aerobic, chemoheterotrophic, free-living, nitrogen-fixing alphaproteobacterium, isolated from a Colombian saline spring.
abstract: A Gram-negative, aerobic, mesophilic, non-spore-forming, chemotrophic, chlorophyll-lacking, nitrogen-fixing bacterium, designated strain USBA 355(T), was isolated from the saline spring 'Salado de Consotá' situated in the Colombian Andes. The non-flagellated cells of strain USBA 355(T) were straight to slightly curved rods (0.6-0.7 x 3.0-3.5 microm). Growth occurred optimally at 30 degrees C (growth temperature range between 20 and 40 degrees C), at pH 6.5-6.7 (pH growth range between 5.0 and 8.0) and at 0.5 % NaCl (w/v) (range between 0 and 4 %). The major quinone present was Q-10 and the predominant fatty acids identified were C(19 : 0) cyclo omega8c, C(18 : 1)omega7c and C(18 : 0). The G+C content of the chromosomal DNA was 71+/-1 mol%. 16S rRNA gene sequence analysis indicated that strain USBA 355(T) formed a distant phylogenetic line of descent with members of the genus Thalassobaculum, family Rhodospirillaceae, class Alphaproteobacteria (90 % gene sequence similarity). Comparison of the phylogenetic, chemotaxonomic and physiological features of strain USBA 355(T) with all other members of the family Rhodospirillaceae suggested that it represents a novel genus and species for which the name Tistlia consotensis gen. nov., sp. nov. is proposed. The type strain of the type species is USBA 355(T) (=JCM 15529(T)=KCTC 22406(T)).
authors: Unidad de Saneamiento y Biotecnología Ambiental, Departamento de Biología, Pontificia Universidad Javeriana, POB 56710, Bogotá, Colombia.
authors: C
authors: C
authors: Díaz-Cárdenas
authors: Microbial Gene Research and Resources Facility, School of Biomolecular and Physical Sciences, Griffith University, Brisbane 4111, Australia.
authors: B K C
authors: BKC
authors: Patel
authors: Unidad de Saneamiento y Biotecnología Ambiental, Departamento de Biología, Pontificia Universidad Javeriana, POB 56710, Bogotá, Colombia.
authors: S
authors: S
authors: Baena
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D003105:Colombia; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D005798:Genes, Bacterial; D045482:Hot Springs / Q000382:microbiology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial