title: Dynamics of Nampt/visfatin and high molecular weight adiponectin in response to oral glucose load in obese and lean women.
abstract: BACKGROUND
  High molecular weight adiponectin (HMWA) is the active circulating form of adiponectin. Nampt/visfatin is the enzyme secreted from adipocytes in an active form and is one of the putative regulators of insulin secretion.
  
  
  OBJECTIVE
  To investigate the dynamics of total adiponectin (TA), HMWA and Nampt/visfatin in obese and lean women during oral glucose tolerance test (OGTT).
  
  
  METHODS
  We studied normal glucose-tolerant (NGT), age-matched, 30 obese and 30 lean women. All subjects underwent a standard 75 g, 2-h OGTT, and area under the curve (AUC) during OGTT for glucose, insulin, Nampt/visfatin, TA and HMWA was calculated. Body fat mass was assessed by bioimpedance analysis. Results Obese women had significantly higher basal and AUC values for insulin and Nampt/visfatin, whereas basal and AUC-HMWA were significantly lower in this group. Alternatively, obese and lean groups had similar basal and AUC values for glucose and TA. Basal insulin levels were negatively correlated with HMWA levels, but not with basal Nampt/visfatin. AUC-insulin was correlated positively with AUC-visfatin, and negatively with AUC-HMWA. Total and truncal body fat mass showed positive correlation with basal and AUC-visfatin, and negative correlation with basal and AUC-HMWA.
  
  
  CONCLUSIONS
  In the NGT state, obese women have higher Nampt/visfatin and lower HMWA levels, both basally and in response to oral glucose challenge. The dynamics of Nampt/visfatin and HMWA during OGTT appear to be linked with insulin and adiposity. Counter-regulatory adaptations in HMWA and Nampt/visfatin might have an impact on suggested adipoinsular axis, contributing to maintenance of normal glucose tolerance.
authors: Department of Internal Medicine, Endocrinology and Metabolism Unit, Hacettepe University School of Medicine, Hacettepe, Ankara, Turkey. ugurunluturk@gmail.com
authors: Uğur
authors: U
authors: Unlütürk
authors: Ayla
authors: A
authors: Harmanci
authors: Bülent Okan
authors: BO
authors: Yildiz
authors: Miyase
authors: M
authors: Bayraktar
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D015992:Body Mass Index; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D009765:Obesity / Q000503:physiopathology*; D013851:Thinness / Q000503:physiopathology*
chemical_list: D052242:Adiponectin; D007328:Insulin; D054409:Nicotinamide Phosphoribosyltransferase