The complete genomic sequence of an in vivo low replicating BLV strain.
DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.
Dube
Syamalima
S
Department of Medicine, Upstate Medical University, Syracuse, New York 13210, USA. dubes@upstate.edu
Abbott
Lynn
L
Dube
Dipak K
DK
Dolcini
Guillermina
G
Gutierrez
Silvina
S
Ceriani
Carolina
C
Juliarena
Marcela
M
Ferrer
Jorge
J
Perzova
Raisa
R
Poiesz
Bernard J
BJ
D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina; D001483:Base Sequence; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016583:Enzootic Bovine Leukosis / Q000821:virology; D016679:Genome, Viral; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000502:physiology; D008214:Lymphocytes / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014779:Virus Replication
D004279:DNA, Viral