Correlation between proliferation markers: PCNA, Ki-67, MCM-2 and antiapoptotic protein Bcl-2 in colorectal cancer.
BACKGROUND
Colorectal cancer is a major oncological problem. The rate of cancer growth depends on proliferative activity and tumor cell death rate. Therefore, the study objective was to estimate the expression of Bcl-2 as an apoptotic protein representative, as well as other proteins involved in proliferation PCNA (proliferating cell nuclear antigen), Ki-67 and MCM-2 (minichromosome maintaince), and to analyze the correlations between them and chosen anatomo-clinical parameters.


METHODS
The expressions of these proteins were analyzed in 47 patients with pT3, G2 colorectal cancer by immunohistochemistry.


RESULTS
No association was found between the expressions of Bcl-2, PCNA, Ki-67, MCM-2 and histological type of tumor, distant metastasis, gender or age of patients. However, we observed a correlation of PCNA, Ki-67 and MCM-2 expressions, but not of Bcl-2, in the main mass of tumor in patients with lymph node metastases. Moreover, a very strong relationship was noted between the expression of Bcl-2 and PCNA, Ki-67 and MCM-2 in the main mass of tumor. Conlusion: These investigations seem to suggest that the expression of proliferative proteins (PCNA, Ki-67, MCM-2) in pT3, G2 of colorectal cancer may indicate the development of lymph node metastases and that inhibited apoptosis by Bcl-2 protein can enhance the action of these proteins in tumor progression.
Guzińska-Ustymowicz
Katarzyna
K
Department of General Pathomorphology, Medical University of Białystok, ul. Waszyngtona 13, 15-269 Białystok, Poland. kasia.guzinska@gmail.com
Pryczynicz
Anna
A
Kemona
Andrzej
A
Czyzewska
Jolanta
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000378:metabolism*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2