title: The effect of Bacillus subtilis mouth rinsing in patients with periodontitis.
abstract: Bacillus subtilis is an effective probiotic product for prevention of enteric infections both in humans and animals. We hypothesized that a mouth rinse containing Bacillus subtilis should adhere to and colonize part of the oral bacteria on periodontal tissue. The rinsing ability of Extraction 300E (containing Bacillus subtilis: E-300) was compared with that of a mouth wash liquid , Neosteline Green (benzethonium chloride; NG) that is commonly used in Japan. Compared with NG rinsing, E-300 rinsing resulted in a marked change in the BANA-score. The mean BANA values (score +/- SD) over the course of the study from 0 to 30 days were 1.52 +/- 0.51 (p < or = 0.1) and 0.30 +/- 0.47 (p < or = 0.01) for E-300, and 1.56 +/- 0.51 and 0.93 +/- 0.68 for NG, respectively. Gingival Index also had improvement, while probing pocket depth and bleeding on probing showed small improvements. Mouth rinsing with E-300 significantly reduced periodontal pathogens compared with NG. These results suggest that Bacillus subtilis is an appropriate mouth rinse for patients with periodontitis.
authors: Tsubura Dental Clinic, 9-6 Enomachi, Utsunomiya, Tochigi, 320-0802, Japan. tshu@ucatv.ne.jp
authors: S
authors: S
authors: Tsubura
authors: H
authors: H
authors: Mizunuma
authors: S
authors: S
authors: Ishikawa
authors: I
authors: I
authors: Oyake
authors: M
authors: M
authors: Okabayashi
authors: K
authors: K
authors: Katoh
authors: M
authors: M
authors: Shibata
authors: T
authors: T
authors: Iizuka
authors: T
authors: T
authors: Toda
authors: T
authors: T
authors: Iizuka
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology*; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010518:Periodontitis / Q000473:pathology / Q000628:therapy*; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*