title: Genomics of helper component proteinase reveals effective strategy for papaya ringspot virus resistance.
abstract: Papaya ringspot virus (PRSV) causes severe economic losses in both cucurbits and papaya throughout the tropics and subtropics. Development of PRSV-resistant transgenic plants faces a major hurdle in achieving resistance against geographically distinct isolates. One of the major reasons of failing to achieve the broad-spectrum PRSV resistance is the involvement of silencing suppressor proteins of viral origin. Here, based on sequence profile of silencing suppressor protein, HcPro, we show that PRSV-HcPro, acts as a suppressor of RNA silencing through micro RNA binding in a dose- dependent manner. In planta expression of PRSV-HcPro affects developmental biology of plants, suggesting the interference of suppressor protein in micro RNA-directed regulatory pathways of plants. Besides facilitating the establishment of PRSV, it showed strong positive synergism with other heterologous viruses as well. This study provides a strategy to develop effective and stable PRSV-resistant transgenic plants.
authors: Plant Pathology Section, Directorate of Rice Research, Hyderabad, 30, India.
authors: Satendra K
authors: SK
authors: Mangrauthia
authors: Priyanka
authors: P
authors: Singh
authors: Shelly
authors: S
authors: Praveen
mesh_terms: D029441:Carica / Q000502:physiology* / Q000821:virology*; D003546:Cysteine Endopeptidases / Q000235:genetics*; D020868:Gene Silencing; D024861:Genetic Enhancement / Q000379:methods*; D018745:Genome, Plant / Q000235:genetics; D010935:Plant Diseases / Q000517:prevention & control* / Q000821:virology*; D019176:Potyviridae / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus