title: Detection of human papillomavirus and adenovirus in benign and malignant lesions of the larynx.
abstract: OBJECTIVE
  To investigate the role of human papillomavirus (HPV) and adenovirus (AdV) infections in the oncogenesis of squamous cell carcinomas of the larynx and of laryngeal dysplasia.
  
  
  METHODS
  Cross-sectional study with planned data collection.
  
  
  METHODS
  Department of Otorhinolaryngology G. Ferreri and Department of Experimental Medicine and Pathology-Section of Virology, Sapienza University of Rome.
  
  
  METHODS
  Biopsy samples were taken from 68 patients with benign and malignant lesions of the larynx. All tissue samples were analyzed by means of polymerase chain reaction with two groups of primers for HPV and with a pair of primers for AdV.
  
  
  RESULTS
  All cases of carcinomas and dysplasia as well as all control cases were negative for both viruses. Four of the five cases of laryngeal papillomas were positive for only HPV, confirming the role of these viral types in the origin of papillomas.
  
  
  CONCLUSIONS
  The absence of viral genomes in laryngeal carcinomas as in the other cases studied suggests the existence of other factors that play a more important role than viral infection in the carcinogenesis of these lesions.
authors: Department of Otorhinolaryngology, G. Ferreri, Sapienza University, Rome, Italy. andrea.gallo@uniroma.it
authors: Andrea
authors: A
authors: Gallo
authors: Anna Marta
authors: AM
authors: Degener
authors: Giulio
authors: G
authors: Pagliuca
authors: Alessandra
authors: A
authors: Pierangeli
authors: Federica
authors: F
authors: Bizzoni
authors: Antonio
authors: A
authors: Greco
authors: Marco
authors: M
authors: de Vincentiis
mesh_terms: D000046:Academic Medical Centers; D000256:Adenoviridae / Q000302:isolation & purification*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000821:virology; D007818:Laryngeal Diseases / Q000821:virology; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012307:Risk Factors; D012384:Rome; D014412:Tumor Virus Infections / Q000150:complications* / Q000821:virology