title: Development and biocompatibility of a novel corrodible fluoride-coated magnesium-calcium alloy with improved degradation kinetics and adequate mechanical properties for cardiovascular applications.
abstract: Recently, corrodible magnesium-based alloys have been introduced for use as cardiovascular stents and orthopedic implants. However, rapid corrosion rates have raised questions about their biocompatibility. Therefore, we developed a binary fluoride-coated magnesium-calcium alloy with improved degradation kinetics. Biocompatibility of the alloys was evaluated with metabolic assays (colorimetric WST-1 test). Furthermore, five different probes of magnesium-calcium alloys (MgCa 0.4, 0.6, 0.8, 1.2, and 2.0 wt %) were cocultivated with human smooth muscle cells and endothelial cells. To investigate the decomposition kinetics in a physiological environment the alloys were used untreated and fluoride coated (MgF(2)). Mg and Ca decreased the metabolic activity in vascular cells dose-dependently, with cytotoxic effects only at unphysiological concentrations. Uncoated magnesium alloys showed signs of decomposition after a short incubation time of 24 h in contrast to MgF(2) coated alloys. After 10 days smooth muscle and endothelial cells around the alloys were still alive, whereas colonization of the surfaces was only observed for smooth muscle cells. The fluoride-coated MgCa alloys exhibited good results concerning mechanical properties, degradation kinetics, and biocompatibility in vitro. We conclude that a binary fluoride magnesium-calcium alloy is a promising candidate for the production of cardiovascular stents.
authors: Department of Pediatric Cardiology and Intensive Care, Children's Hospital, University of Rostock, Rembrandtstrasse 16/17, 18057 Rostock, Germany.
authors: Andreas
authors: A
authors: Drynda
authors: Thomas
authors: T
authors: Hassel
authors: René
authors: R
authors: Hoehn
authors: Angela
authors: A
authors: Perz
authors: Friedrich-Wilhelm
authors: FW
authors: Bach
authors: Matthias
authors: M
authors: Peuster
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D003343:Corrosion; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005459:Fluorides / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D015607:Stents; D013499:Surface Properties
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D008274:Magnesium; D005459:Fluorides; D002118:Calcium