title: Variation of M3 muscarinic receptor expression in different prostate tissues and its significance.
abstract: OBJECTIVE
  To detect the expression of the muscarinic receptor (M receptor) in different prostate tissues and analyze the role of its subtype in prostatic oncogenesis.
  
  
  METHODS
  Thirty-six cases of normal prostate and benign prostatic hyperplasia, and 8 cases of prostatic tumor, were used in this study from the Shandong University, Shandong, China, between 2003-2006. The protein expressions of M1, M2, and M3 receptors in each group were determined by Western-blotting. The gene expressions of the M3 receptor and vascular endothelial growth factors (VEGF) in each group were determined by reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  The protein and gene expressions of the M3 receptor in the prostatic carcinoma group were higher than that of benign prostatic hyperplasia group (p=0.0001) and normal prostate group (p=0.0001). The M3 receptor and VEGF showed positive straight-line correlations of gene expressions with the 3 groups (r=0.4999, p=0.0001).
  
  
  CONCLUSIONS
  The M3 receptor may have a close relationship with prostatic oncogenesis.
authors: Department of Minimally Invasive Urology Center, Shandong Provincial Hospital, The Second Hospital of Shandong University, Jinan, China.
authors: Wei
authors: W
authors: Song
authors: Mingzhen
authors: M
authors: Yuan
authors: Shengtian
authors: S
authors: Zhao
mesh_terms: D000368:Aged; D015153:Blotting, Western; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D043643:Receptor, Muscarinic M1 / Q000378:metabolism; D043585:Receptor, Muscarinic M2 / Q000378:metabolism; D043587:Receptor, Muscarinic M3 / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D043643:Receptor, Muscarinic M1; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D042461:Vascular Endothelial Growth Factor A