title: Cell-selective metabolic labeling of proteins.
abstract: Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.
authors: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.
authors: John T
authors: JT
authors: Ngo
authors: Julie A
authors: JA
authors: Champion
authors: Alborz
authors: A
authors: Mahdavi
authors: I Caglar
authors: IC
authors: Tanrikulu
authors: Kimberly E
authors: KE
authors: Beatty
authors: Rebecca E
authors: RE
authors: Connor
authors: Tae Hyeon
authors: TH
authors: Yoo
authors: Daniela C
authors: DC
authors: Dieterich
authors: Erin M
authors: EM
authors: Schuman
authors: David A
authors: DA
authors: Tirrell
mesh_terms: D000345:Affinity Labels / Q000378:metabolism*; D000409:Alanine / Q000031:analogs & derivatives / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000382:microbiology; D008718:Methionine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009646:Norleucine / Q000031:analogs & derivatives / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D011506:Proteins; D009646:Norleucine; D000604:Amino Acyl-tRNA Synthetases; D008718:Methionine-tRNA Ligase; D000409:Alanine