title: Heterologous prime/boost immunizations of rhesus monkeys using chimpanzee adenovirus vectors.
abstract: Pre-existing immunity to human adenovirus serotype 5 (AdHu5) has been shown to suppress the immunogenicity of recombinant Ad5 (rAdHu5) vector-based vaccines for human immunodeficiency virus type 1 (HIV-1) in both preclinical studies and clinical trials. As a potential solution to this problem we developed adenovirus vaccine vectors of chimpanzee origin. In the present study we assessed the immunogenicity of various chimpanzee adenovirus vectors in a prime/boost regimen to HIV-1 envelope and SIV Gag-Pol in rhesus monkeys and their ability to protect against pathogenic viral challenge. Although rAdHu5-primed monkeys had higher magnitude T cell responses than rAdC7 or rAdC68 prior to challenge, the rAdC7-rAdC1/C5 and rAdHu5-rAdC1/C5 immunizations resulted in comparable magnitude recall cellular immune responses and comparable level of control of viremia post-challenge.
authors: Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
authors: Sampa
authors: S
authors: Santra
authors: Yue
authors: Y
authors: Sun
authors: Birgit
authors: B
authors: Korioth-Schmitz
authors: Julie
authors: J
authors: Fitzgerald
authors: Cherie
authors: C
authors: Charbonneau
authors: Giannina
authors: G
authors: Santos
authors: Michael S
authors: MS
authors: Seaman
authors: Sarah J
authors: SJ
authors: Ratcliffe
authors: David C
authors: DC
authors: Montefiori
authors: Gary J
authors: GJ
authors: Nabel
authors: Hildegund C J
authors: HC
authors: Ertl
authors: Norman L
authors: NL
authors: Letvin
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000260:Adenoviruses, Human / Q000276:immunology; D000261:Adenoviruses, Simian / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005822:Genetic Vectors / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D008253:Macaca mulatta; D009500:Neutralization Tests; D002679:Pan troglodytes / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral; D016207:Cytokines; D012367:RNA, Viral; D054299:env Gene Products, Human Immunodeficiency Virus