title: Site-specific two-color protein labeling for FRET studies using split inteins.
abstract: Fluorescence resonance energy transfer (FRET) is an ideal tool for studying protein conformational changes and dynamics. Strategies to specifically label proteins with multiple colors at structurally important sites, a prerequisite for such studies, however, need to be devised on a protein-by-protein basis. Here we describe a simple yet widely applicable two-color site-specific protein labeling method utilizing Npu DnaE split intein.
authors: Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan.
authors: Jin-Yi
authors: JY
authors: Yang
authors: Wei Yuan
authors: WY
authors: Yang
mesh_terms: D001665:Binding Sites; D003116:Color; D003545:Cysteine; D004258:DNA Polymerase III / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D047668:Inteins; D046937:Nostoc / Q000201:enzymology; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D019154:Protein Splicing; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D013194:Staining and Labeling; D013379:Substrate Specificity
chemical_list: D010446:Peptide Fragments; D011506:Proteins; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III; D003545:Cysteine