[DNA-PKcs silencing inhibit the DNA repair induced by low dose radiation on human breast epithelial cells].
DNA-PKcs, the catalytic subunit of DNA-dependent protein kinase (DNA-PK), plays an important role in the nonhomologous end-joining (NHEJ) pathway of DNA double-strand breaks (DSBs) repair. To investigate the effects of DNA-PKcs down-regulation on cell growth and sensitization to low dose radiation (LDR), we transfected DNA-PKcs siRNA into human mammary epithelia cells MCF10F, then, detected the proliferation curve of the cells and the expression of protiens in DNA repair pathways. The results showed that DNA-PKcs gene silencing, induced by the transfection of DNA-PKcs siRNA could suppress significantly the cell proliferation and inhibit the expression of the DNA repair proteins, such as Ku80, ATM and P53 after 50 cGy 137Cs gamma-irradiation.The results suggested that DNA-PKcs gene silencing could increase the sensitivity of human breast epithelial cells to the LDR, which might be relative with the decrease of the proteins.
Zou
Wei
W
College of Life Sciences, Liaoning Normal University, Dalian 116029, China. weizou60@hotmail.com
Che
Jian
J
Wang
Chongjie
C
Cui
Yuying
Y
Zhang
Qinming
Q
D001940:Breast / Q000166:cytology*; D002460:Cell Line; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D051747:DNA-Activated Protein Kinase / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D004847:Epithelial Cells / Q000378:metabolism / Q000528:radiation effects*; D020868:Gene Silencing; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human