Heterologous prime/boost immunizations of rhesus monkeys using chimpanzee adenovirus vectors.
Pre-existing immunity to human adenovirus serotype 5 (AdHu5) has been shown to suppress the immunogenicity of recombinant Ad5 (rAdHu5) vector-based vaccines for human immunodeficiency virus type 1 (HIV-1) in both preclinical studies and clinical trials. As a potential solution to this problem we developed adenovirus vaccine vectors of chimpanzee origin. In the present study we assessed the immunogenicity of various chimpanzee adenovirus vectors in a prime/boost regimen to HIV-1 envelope and SIV Gag-Pol in rhesus monkeys and their ability to protect against pathogenic viral challenge. Although rAdHu5-primed monkeys had higher magnitude T cell responses than rAdC7 or rAdC68 prior to challenge, the rAdC7-rAdC1/C5 and rAdHu5-rAdC1/C5 immunizations resulted in comparable magnitude recall cellular immune responses and comparable level of control of viremia post-challenge.
Santra
Sampa
S
Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Sun
Yue
Y
Korioth-Schmitz
Birgit
B
Fitzgerald
Julie
J
Charbonneau
Cherie
C
Santos
Giannina
G
Seaman
Michael S
MS
Ratcliffe
Sarah J
SJ
Montefiori
David C
DC
Nabel
Gary J
GJ
Ertl
Hildegund C J
HC
Letvin
Norman L
NL
D016915:AIDS Vaccines / Q000276:immunology*; D000260:Adenoviruses, Human / Q000276:immunology; D000261:Adenoviruses, Simian / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005822:Genetic Vectors / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D008253:Macaca mulatta; D009500:Neutralization Tests; D002679:Pan troglodytes / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D016207:Cytokines; D012367:RNA, Viral; D054299:env Gene Products, Human Immunodeficiency Virus