Side-chain structure of cell surface polysaccharide, mannan, affects hypocholesterolemic activity of yeast.
We previously reported that Kluyveromyces marxianus YIT 8292 exhibited more potent hypocholesterolemic activity than other yeasts containing Saccharomyces cerevisiae . To clarify the reason for the higher hypocholesterolemic activity, we examined the side-chain structure of cell surface polysaccharide, mannan, of K. marxianus YIT 8292. The result shows that K. marxianus YIT 8292 had shorter alpha-(1,2)-linked oligomannosyl side chains and lower phosphate content in mannan than S. cerevisiae. The association between its structural features and hypocholesterolemic activity was investigated by comparing the hypocholesterolemic activities of S. cerevisiae mannan mutants in rats fed a high-cholesterol diet. S. cerevisiae mnn5 mutant with deficiencies in the phosphorylation and elongation of mannan side chains showed higher hypocholesterolemic activity than the wild-type strain. These results show that the side-chain length and phosphate contents of mannan affect hypocholesterolemic activity.
Yoshida
Yasuto
Y
Yakult Central Institute for Microbiological Research, Kunitachi-shi, Tokyo 186-8650, Japan. yasuto-yoshida@yakult.co.jp
Naito
Eiichiro
E
Mizukoshi
Harumi
H
Watanabe
Yoko
Y
Kimura
Kazumasa
K
Yokoi
Wakae
W
Sato
Tadashi
T
Okumura
Takekazu
T
Ito
Masahiko
M
Sawada
Haruji
H
D000818:Animals; D000924:Anticholesteremic Agents; D002473:Cell Wall / Q000737:chemistry*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D007716:Kluyveromyces / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D008055:Lipids / Q000097:blood; D008297:Male; D008351:Mannans / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D010710:Phosphates / Q000032:analysis; D019936:Probiotics / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D013329:Structure-Activity Relationship
D000924:Anticholesteremic Agents; D002791:Cholesterol, Dietary; D008055:Lipids; D008351:Mannans; D010710:Phosphates