Stable surface expression of a gene for Helicobacter pylori toxic porin protein with pBAD expression system.
Helicobacter pylori (H. pylori) infection causes peptic and duodenal diseases in humans. Among a 32-protein family of outer membrane proteins, a porin-like protein, HopE, has been a subject of note, mainly for its conservative nature among H. pylori, and for its potential as a vaccine candidate. To achieve stable surface expression of this host cell-toxic protein, hopE gene was introduced into pBAD expression system. After induction with arabinose, all 15 randomly-chosen E. coli LMG 194 colonies from 3 successive passages could express HopE protein, while only 1 from 5 E. coli colonies that contained lac operon-regulated plasmid encoding hopE gene could express HopE. Indirect immunofluorescence confirmed the expression of HopE on E. coli cell surface.
Peng
Zhixiang
Z
Department of Endodontics, Guanghua College of Stomatology, Sun Yat-Sen University, Guangzhou, 510055, China. pengzhixiang@gmail.com
Wei
Xi
X
Lin
Zhengmei
Z
D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D002352:Carrier Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology; D018272:Porins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D000942:Antigens, Bacterial; C016242:AraF protein, E coli; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D018272:Porins; D011994:Recombinant Proteins