title: Transepithelial transport of Cerulenin across Caco-2 cell monolayers.
abstract: The transepithelial transport of Cerulenin across Caco-2 cell monolayers was examined in this study. The permeated amounts of Cerulenin were measured by HPLC method to calculate the permeation rate and the apparent permeability coefficient (P(app)). The transport of Cerulenin was independent on apical pH and exhibited concentration-dependent and nonsatuable even at 10 mM Cerulenin. The permeation rate at 1 mM Cerulenin in the apical-to-basolateral direction was 0.151 ng/min/mg of protein and the P(app) was 3.76 x 10(-6) cm/second. The permeation rate of Cerulenin was affected by neither metabolic inhibitors nor inhibitors for P-glycoprotein, as was the same case in monolayers treated with cytochalasin D. All these data from experiments indicated that transport of Cerulenin across Caco-2 cell monolayers was not mediated by ATP-dependent transport systems nor via paracellular pathway, but via passive diffusion without efflux by P-glycoprotein.
authors: Department of Medicine, Zhangzhou Health College, Zhangzhou Fujian, P.R. China.
authors: Da-Hua
authors: DH
authors: Fu
authors: Zhi-Li
authors: ZL
authors: Liu
authors: Jun-Shi
authors: JS
authors: Liu
authors: Yun
authors: Y
authors: Luo
authors: Yong
authors: Y
authors: Shu
authors: Shan-Hu
authors: SH
authors: Huang
authors: Zhi-Min
authors: ZM
authors: Han
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D001692:Biological Transport; D018938:Caco-2 Cells; D002569:Cerulenin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D054872:Fatty Acid Synthesis Inhibitors / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D010539:Permeability
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D054872:Fatty Acid Synthesis Inhibitors; D002569:Cerulenin