Brucella inopinata sp. nov., isolated from a breast implant infection.
A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1(T)) was isolated recently from a breast implant infection of a 71-year-old female patient with clinical signs of brucellosis. Affiliation of strain BO1(T) to the genus Brucella was confirmed by means of polyamine pattern, polar lipid profile, fatty acid profile, quinone system, DNA-DNA hybridization studies and by insertion sequence 711 (IS711)-specific PCR. Strain BO1(T) harboured four to five copies of the Brucella-specific insertion element IS 711, displaying a unique banding pattern, and exhibited a unique 16S rRNA gene sequence and also grouped separately in multilocus sequence typing analysis. Strain BO1(T) reacted with Brucella M-monospecific antiserum. Incomplete lysis was detected with bacteriophages Tb (Tbilisi), F1 and F25. Biochemical profiling revealed a high degree of enzymic activity and metabolic capabilities. In multilocus VNTR (variable-number tandem-repeat) analysis, strain BO1(T) showed a very distinctive profile and clustered with the other 'exotic' Brucella strains, including strains isolated from marine mammals, and Brucella microti, Brucella suis biovar 5 and Brucella neotomae. Comparative omp2a and omp2b gene sequence analysis revealed the most divergent omp2 sequences identified to date for a Brucella strain. The recA gene sequence of strain BO1(T) differed in seven nucleotides from the Brucella recA consensus sequence. Using the Brucella species-specific multiplex PCR assay, strain BO1(T) displayed a unique banding pattern not observed in other Brucella species. From the phenotypic and molecular analysis it became evident that strain BO1( T) was clearly different from all other Brucella species, and therefore represents a novel species within the genus Brucella. Because of its unexpected isolation, the name Brucella inopinata with the type strain BO1(T) (=BCCN 09-01(T)=CPAM 6436(T)) is proposed.
Scholz
Holger C
HC
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany.
Nöckler
Karsten
K
Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, Germany.
Göllner
Cornelia
C
Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, Germany.
Bahn
Peter
P
Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, Germany.
Vergnaud
Gilles
G
Université Paris-Sud 11, CNRS, UMR8621, Institut de Génétique et Microbiologie, 91405 Orsay, France.|DGA/D4S - Mission pour la Recherche et l'Innovation Scientifique, 7, rue des Mathurins, 92220 Bagneux, France.
Tomaso
Herbert
H
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany.
Al Dahouk
Sascha
S
RWTH Aachen University, Department of Internal Medicine III, Pauwelsstraße 30, D-52074 Aachen, Germany.
Kämpfer
Peter
P
Institute for Applied Microbiology, Justus-Liebig-Universitat Giessen, IFZ, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Cloeckaert
Axel
A
INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, F-37380 Nouzilly, France.
Maquart
Marianne
M
INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, F-37380 Nouzilly, France.
Zygmunt
Michel S
MS
INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, F-37380 Nouzilly, France.
Whatmore
Adrian M
AM
Veterinary Laboratories Agency, Woodham Lane, Addlestone KT15 3NB, UK.
Pfeffer
Martin
M
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany.
Huber
Birgit
B
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Busse
Hans-Jürgen
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
De
Barun Kumar
BK
Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA.
D000368:Aged; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D019928:Breast Implantation / Q000009:adverse effects; D018427:Breast Implants / Q000382:microbiology*; D002002:Brucella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002006:Brucellosis / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D020459:Genes, rRNA; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D001425:Bacterial Outer Membrane Proteins; D004275:DNA, Ribosomal; D005227:Fatty Acids; C063931:Omp2 protein, bacteria; D012336:RNA, Ribosomal, 16S