Deefgea chitinilytica sp. nov., isolated from a wetland.
A bacterial strain, designated Nsw-4(T), was isolated from a water sample of Niao-Song Wetland Park in Taiwan and was characterized by using a polyphasic taxonomic approach. Strain Nsw-4(T) was Gram-negative, aerobic, ivory-coloured, rod-shaped and motile by means of a polar flagellum. Growth occurred at 15-37 degrees C, pH 6.0-8.0 and 0-2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Deefgea and that its closest neighbour was Deefgea rivuli WB 3.4-79(T) (96.9 %). The results of physiological and biochemical tests allowed the clear phenotypic differentiation of this isolate from D. rivuli WB 3.4-79(T). The major fatty acids were C16 : 1omega7c and C16 : 0. The G+C content of the genomic DNA was 53.7 mol%. On the basis of 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain Nsw-4(T) should be classified as representing a novel species and the second member of the genus Deefgea, for which the name Deefgea chitinilytica sp. nov. is proposed. The type strain is Nsw-4(T) (=BCRC 17934(T)=LMG 24817(T)).
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, HaiChuan Rd, NanTzu, Kaohsiung City 811, Taiwan, ROC.
Chung
Yu-Ni
YN
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, HaiChuan Rd, NanTzu, Kaohsiung City 811, Taiwan, ROC.
Chiu
Tzu-Fang
TF
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, HaiChuan Rd, NanTzu, Kaohsiung City 811, Taiwan, ROC.
Cheng
Chih-Yu
CY
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, HaiChuan Rd, NanTzu, Kaohsiung City 811, Taiwan, ROC.
Arun
A B
AB
Yenepoya Research Center, Yenepoya University, University Rd, Deralakatee, Mangalore, Karnataka state, India.
Sheu
Shih-Yi
SY
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, HaiChuan Rd, NanTzu, Kaohsiung City 811, Taiwan, ROC.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006601:Hexoses / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D014871:Water Microbiology; D053833:Wetlands
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004798:Enzymes; D005227:Fatty Acids; D006601:Hexoses