title: A role for AMPK in the inhibition of glucose-6-phosphate dehydrogenase by polyunsaturated fatty acids.
abstract: Both polyunsaturated fatty acids and AMPK promote energy partitioning away from energy consuming processes, such as fatty acid synthesis, towards energy generating processes, such as beta-oxidation. In this report, we demonstrate that arachidonic acid activates AMPK in primary rat hepatocytes, and that this effect is p38 MAPK-dependent. Activation of AMPK mimics the inhibition by arachidonic acid of the insulin-mediated induction of G6PD. Similar to intracellular signaling by arachidonic acid, AMPK decreases insulin signal transduction, increasing Ser(307) phosphorylation of IRS-1 and a subsequent decrease in AKT phosphorylation. Overexpression of dominant-negative AMPK abolishes the effect of arachidonic acid on G6PD expression. These data suggest a role for AMPK in the inhibition of G6PD by polyunsaturated fatty acids.
authors: Department of Biochemistry, West Virginia University, Morgantown, 26506, USA.
authors: Alison B
authors: AB
authors: Kohan
authors: Indrani
authors: I
authors: Talukdar
authors: Callee M
authors: CM
authors: Walsh
authors: Lisa M
authors: LM
authors: Salati
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D000818:Animals; D016718:Arachidonic Acid / Q000096:biosynthesis / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology*; D007328:Insulin / Q000378:metabolism; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D008297:Male; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D005231:Fatty Acids, Unsaturated; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D016718:Arachidonic Acid; D012694:Serine; D005954:Glucosephosphate Dehydrogenase; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases