[Effect of Panax notoginseng saponins on syp and tau gene expression in brain of senescence accelerated mouse].
OBJECTIVE
To study the effect of Panax notoginseng saponins (PNS) on (synaptophysin, syp) and tau gene expression in the brain tissue in senescence accelerated mouse prone 8 (SAMP 8).


METHODS
SAMP8 were randomly divided into 4 groups: PNS 23.38, 93.50 mg x kg(-1) group, huperzin A 0.038 6 mg x kg(-1) x d(-1) group and blank control group; the drug groups were treated with the designed drugs respectively per day by intragastric administration for 4 consecutive weeks, and double distilled water was given to blank control group. After treatment, the mRNA content of tau and syp were assayed by reverse transcription (RT) and real-time polymerase chain reaction (real-time PCR).


RESULTS
Compared with blank control group, the syp mRNA contents were increased in PNS groups (P < 0.05 or P < 0.01), and the tau mRNA content were not significant difference in all groups.


CONCLUSIONS
This study suggests that PNS can up-regulate syp gene expression at transcriptional level in the brain of SAMP 8.
Lv
Liang
L
Center of Research and Development of New Drugs, Guangxi Chinese Traditional Medical University, Nanning 530001, China.
Zhong
Zhenguo
Z
Wu
Dengpan
D
Chai
Limin
L
Zhang
Wenyan
W
D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D053723:Panax notoginseng / Q000737:chemistry*; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D016875:tau Proteins / Q000235:genetics / Q000378:metabolism
D012503:Saponins; D016875:tau Proteins; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; C516725:Ptpn11 protein, mouse