title: Apolipophorin III interaction with model membranes composed of phosphatidylcholine and sphingomyelin using differential scanning calorimetry.
abstract: Apolipophorin III (apoLp-III) from Locusta migratoria was employed as a model apolipoprotein to gain insight into binding interactions with lipid vesicles. Differential scanning calorimetry (DSC) was used to measure the binding interaction of apoLp-III with liposomes composed of mixtures of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and sphingomyelin (SM). Association of apoLp-III with multilamellar liposomes occurred over a temperature range around the liquid crystalline phase transition (L(alpha)). Qualitative and quantitative data were obtained from changes in the lipid phase transition upon addition of apoLp-III. Eleven ratios of DMPC and SM were tested from pure DMPC to pure SM. Broadness of the phase transition (T(1/2)), melting temperature of the phase transition (T(m)) and enthalpy were used to determine the relative binding affinity to the liposomes. Multilamellar vesicles composed of 40% DMPC and 60% SM showed the greatest interaction with apoLp-III, indicated by large T(1/2) values. Pure DMPC showed the weakest interaction and liposomes with lower percentage of DMPC retained domains of pure DMPC, even upon apoLp-III binding indicating demixing of liposome lipids. Addition of apoLp-III to rehydrated liposomes was compared to codissolved trials, in which lipids were rehydrated in the presence of protein, forcing the protein to interact with the lipid system. Similar trends between the codissolved and non-codissolved trials were observed, indicating a similar binding affinity except for pure DMPC. These results suggested that surface defects due to non-ideal packing that occur at the phase transition temperature of the lipid mixtures are responsible for apolipoprotein-lipid interaction in DMPC/SM liposomes.
authors: Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.
authors: Michael H
authors: MH
authors: Chiu
authors: Chung-Ping Leon
authors: CP
authors: Wan
authors: Paul M M
authors: PM
authors: Weers
authors: Elmar J
authors: EJ
authors: Prenner
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000737:chemistry / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism*; D007202:Indicators and Reagents / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers; D008081:Liposomes; D048188:Locusta migratoria / Q000737:chemistry; D013109:Sphingomyelins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001053:Apolipoproteins; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D008081:Liposomes; D013109:Sphingomyelins; C042470:apolipophorin III; D004134:Dimyristoylphosphatidylcholine