Urinary oligosaccharides: a peripheral marker for Sida carpinifolia exposure or poisoning.
Poisoning by Sida carpinifolia belongs to a group of plant-induced phenotype which resembles lysosomal storage diseases. Saanen goats were fed aerial parts of green S. carpinifolia for up to 3 months. Concentrates complemented the nutritional requirements. Urine and blood samples were collected for oligosaccharide study (by thin layer chromatography-TLC) and hemogram analysis, respectively. Abnormal excretion of oligosaccharides was observed from the 2nd day of S. carpinifolia ingestion until one day after withdrawal of the plant from the diet. There were no changes in hemogram. Clinical signs were typical of poisoning caused by plants of this group and were seen from the 37th day on S. carpinifolia diet until seven days after withdrawal of the plant, when signs gradually became scarce and less evident. Results presented here suggest that detection of urinary oligosaccharides by TLC may be an useful method to assess swainsonine-containing plants exposure or an early diagnostic tool for poisoning by these plants.
Bedin
Marisete
M
Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2350, Serviço de Genética Médica, Bom Fim 90035-903, Porto Alegre-RS, Brazil.
Colodel
Edson M
EM
Giugliani
Roberto
R
Zlotwski
Priscila
P
Cruz
Cláudio E F
CE
Driemeier
David
D
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002855:Chromatography, Thin Layer; D006041:Goats / Q000097:blood / Q000652:urine; D019660:Malvaceae / Q000737:chemistry*; D009844:Oligosaccharides / Q000097:blood / Q000652:urine*; D017026:Swainsonine / Q000506:poisoning*; D014118:Toxins, Biological / Q000506:poisoning*
D015415:Biomarkers; D009844:Oligosaccharides; D014118:Toxins, Biological; D017026:Swainsonine