Georgenia soli sp. nov., isolated from iron-ore-contaminated soil in India.
A Gram-positive actinobacterium (CC-NMPT-T3(T)) was isolated from iron-ore-contaminated soil near New Mangalore Port, Karnataka, India. Based on 16S rRNA gene sequence similarity studies, strain CC-NMPT-T3(T) belongs to the genus Georgenia and is most closely related to Georgenia muralis (98.6 %), Georgenia ruanii (97.4 %) and Georgenia thermotolerans (97.4 %). The peptidoglycan is of the type A4alpha l-Lys<--l-Glu. The predominant isoprenoid quinone is menaquinone MK-8(H(4)) and the polar lipid profile is composed of the predominant compound diphosphatidylglycerol, moderate amounts of a phosphatidylinositol-mannoside, phosphatidylinositol and minor amounts of another phosphatidylmannoside and phosphatidylglycerol. The major fatty acids of strain CC-NMPT-T3(T) are anteiso-C(15 : 0) and iso-C(15 : 0). The results of DNA-DNA hybridizations, and physiological and biochemical tests allowed a clear phenotypic differentiation of strain CC-NMPT-T3(T) from all other Georgenia species. Strain CC-NMPT-T3(T) represents a novel species, for which the name Georgenia soli sp. nov. is proposed. The type strain is CC-NMPT-T3(T) (=DSM 21838(T)=CCM 7658(T)).
Kämpfer
P
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Arun
A B
AB
Yenepoya Research Centre, Yenepoya University, University Road, Deralakatee, Mangalore, Karnataka State, India.
Busse
H-J
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Langer
S
S
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Young
C-C
CC
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Chen
W-M
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
Schumann
P
P
Deutsche Sammlung von Mikroorganismen und Zellkulturen, D-38124 Braunschweig, Germany.
Syed
A A
AA
Yenepoya Research Centre, Yenepoya University, University Road, Deralakatee, Mangalore, Karnataka State, India.
Rekha
P D
PD
Yenepoya Research Centre, Yenepoya University, University Road, Deralakatee, Mangalore, Karnataka State, India.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007194:India; D007501:Iron; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D007501:Iron