title: Characterizing the polymeric status of Helicobacter pylori heat shock protein 60.
abstract: Helicobacter pylori heat shock protein 60 (HpHsp60) was first identified as an adhesion molecule associated with H. pylori infection. Here we have analyzed the structure of HpHsp60 via amino acid BLAST, circular dichroism, and electrophoresis and the results indicate that most recombinant HpHsp60 molecules exist as dimers or tetramers, which is quite different from Escherichia coli Hsp60. Treatment of human monocytic cells THP-1 with HpHsp60 was found to up-regulate a panel of cytokines including IL-1alpha, IL-8, IL-10, IFN-gamma, TNF-alpha, TGF-beta, GRO, and RANTES. Carboxymethylated HpHsp60 molecules with a switched oligomeric status were able to further enhance NF-kappaB-mediated IL-8 and TNF-alpha secretion in THP-1 cells compared to unmodified HpHsp60 molecules. These results indicated that the oligomeric status of HpHsp60s might have an important role in regulating host inflammation and thus help facilitate H. pylori persistent infection.
authors: Department of Biological Science and Technology, National Chiao-Tung University, Hsin-Chu, Taiwan.
authors: Ching-Yi
authors: CY
authors: Lin
authors: Yu-Shan
authors: YS
authors: Huang
authors: Chi-Han
authors: CH
authors: Li
authors: Yuan-Ting
authors: YT
authors: Hsieh
authors: Nu-Man
authors: NM
authors: Tsai
authors: Pei-Juin
authors: PJ
authors: He
authors: Wei-Tung
authors: WT
authors: Hsu
authors: Yi-Chen
authors: YC
authors: Yeh
authors: Fang-Hsing
authors: FH
authors: Chiang
authors: Ming-Shiang
authors: MS
authors: Wu
authors: Chia-Ching
authors: CC
authors: Chang
authors: Kuang-Wen
authors: KW
authors: Liao
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D018834:Chaperonin 60 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D003545:Cysteine / Q000737:chemistry; D016207:Cytokines / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011108:Polymers / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: C078096:60K protein, Helicobacter pylori; D001426:Bacterial Proteins; D018834:Chaperonin 60; D016207:Cytokines; D011108:Polymers; D011994:Recombinant Proteins; D003545:Cysteine