title: Elasticity-based patterning of red blood cells on undulated lipid membranes supported on porous topographic substrates.
abstract: We describe elasticity-based patterning of human red blood cells (RBCs) into a microarray form on supported lipid membranes (SLMs) prepared on a solid substrate having two types of topographic patterns, porous and flat regions. The underlying concept is to precisely control the interplay between adhesion and the bending rigidity of the RBCs that interact with the SLMs. Attachment of the RBCs on highly undulated SLMs formed on the porous region is not energetically favorable, since membrane bending of the RBCs costs a high curvature elastic energy which exceeds adhesion. The RBCs are thus selectively confined within relatively flat regions of the SLMs without causing considerable elastic distortions. It was found that the population of the RBCs in a single corral is linearly proportional to the area of one element in our microarray.
authors: School of Electrical Engineering #032, Seoul National University, Kwanak, Seoul, South Korea.
authors: Sang-Wook
authors: SW
authors: Lee
authors: Cherlhyun
authors: C
authors: Jeong
authors: Sin-Doo
authors: SD
authors: Lee
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D004548:Elasticity; D004912:Erythrocytes / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D046228:Microarray Analysis; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers