Structural characterisation of the pectic polysaccharide rhamnogalacturonan II using an acidic fingerprinting methodology.
Rhamnogalacturonan II (RG-II) is a structurally complex cell wall pectic polysaccharide. Despite its complexity, both the structure of RG-II and its ability to dimerise via a borate diester are conserved in vascular plants suggesting that RG-II has a fundamental role in primary cell wall organisation and function. The selection and analysis of new mutants affected in RG-II formation represents a promising strategy to unravel these functions and to identify genes encoding enzymes involved in RG-II biosynthesis. In this paper, a novel fingerprinting strategy is described for the screening of RG-II mutants based on the mild acid hydrolysis of RG-II coupled to the analysis of the resulting fragments by mass spectrometry. This methodology was developed using RG-II fractions isolated from citrus pectins and then validated for RG-II isolated from the Arabidopsis mur1 mutant and irx10 irx10-like double mutant.
Séveno
Martial
M
EA 4358, IFRMP 23, University of Rouen, 76821 Mont Saint Aignan, France.
Voxeur
Aline
A
Rihouey
Christophe
C
Wu
Ai-Min
AM
Ishii
Tadashi
T
Chevalier
Christian
C
Ralet
Marie Christine
MC
Driouich
Azeddine
A
Marchant
Alan
A
Lerouge
Patrice
P
D017360:Arabidopsis / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D002957:Citrus / Q000737:chemistry*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D013696:Temperature; D014269:Trifluoroacetic Acid / Q000737:chemistry*
C042492:rhamnogalacturonan II; D010368:Pectins; D014269:Trifluoroacetic Acid