title: Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester.
abstract: Screening was carried out for microorganisms able to produce N-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing APM production was purified and identified as dipeptidase E.
authors: AminoScience Laboratories, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan. ikuo_kira@ajinomoto.com
authors: Ikuo
authors: I
authors: Kira
authors: Yasuhisa
authors: Y
authors: Asano
authors: Kenzo
authors: K
authors: Yokozeki
mesh_terms: D001216:Asparagine / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D002384:Catalysis; D002954:Citrobacter / Q000201:enzymology; D004150:Dipeptidases / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012486:Salmonella typhimurium / Q000201:enzymology; D012763:Shigella flexneri / Q000201:enzymology
chemical_list: D011994:Recombinant Proteins; C034515:phenylalanine methyl ester; D010649:Phenylalanine; D001216:Asparagine; D004150:Dipeptidases