Ad-gBCMVpoly: A novel chimeric vaccine strategy for human cytomegalovirus-associated diseases.
In spite of numerous attempts at successful licensure, a human cytomegalovirus (HCMV) vaccine formulation remains elusive. To overcome the limitations of previous strategies, we have recently developed a novel chimeric vaccine which allows induction of both humoral and cellular immune response following a single vaccination. This vaccine includes the extracellular domain of HCMV-encoded glycoprotein-B (gB) covalently linked to multiple HLA class I- and class II-restricted T-cell epitopes from multiple HCMV antigens as a contiguous polypeptide in a replication-deficient adenoviral vector Ad5/F35 (referred to as Ad-gBCMVpoly). Immunisation with Ad-gBCMVpoly consistently generated strong gB-specific neutralizing antibody and a broad range of HCMV-specific pluripotent CD8(+) and CD4(+) T-cells. This immunity suppressed infection with recombinant vaccinia virus encoding HCMV antigens. Furthermore, in vitro stimulation with Ad-gBCMVpoly rapidly expanded multiple antigen-specific human CD8(+) and CD4(+) T-cells from healthy virus carriers. Here we discuss the advantages of the Ad-gBCMVpoly vaccine and its potential application in different clinical settings.
Zhong
Jie
J
Australian Centre for Vaccine Development, Queensland Institute of Medical Research, Brisbane, Australia.
Khanna
Rajiv
R
D000256:Adenoviridae; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology / Q000517:prevention & control*; D022482:Cytomegalovirus Vaccines / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005822:Genetic Vectors; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D022482:Cytomegalovirus Vaccines; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus