Effects of Teladorsagia (Ostertagia) circumcincta infection on lambs selected for high fleece weight.
The physiological processes leading to the expression of the resilient phenotype, which allow animals to maintain a relatively higher production level during infection, have been investigated in lambs from a closed flock selected for 40 generations for high fleece weight (HFW), but with higher FEC and worm burdens than their unselected control (C) flock run in parallel. After recovery from surgery to implant abomasal cannulae, eight parasite-naive lambs from each flock were infected intraruminally at 4.5 months-of-age with 50,000 Teladorsagia circumcincta L3. Blood, abomasal fluid and faecal samples were collected daily for measurement of serum gastrin and pepsinogen concentrations, blood eosinophils, abomasal pH and FEC. Four lambs from each flock were euthanased on Day 8 post-infection and the other four on Day 28 post-infection. At necropsy, abomasal contents and tissues were collected for worm counts, abomasal lymph nodes and fundic tissue for cytokine gene expression and fundic tissue for histopathology. Expression of resilience appeared to be age-dependent as there were no significant differences in either FEC or worm burden between lambs from the two flocks, unlike older HFW lambs in a previous study. Abomasal secretion did not differ between flocks. Histopathological changes were typical of parasitism: inflammatory cells, mainly eosinophils and lymphocytes, were numerous in nodular areas and there were fewer TGF-alpha positive parietal cells, many of which were vacuolated. By Day 28 p.i., globule leucocytes were present. Mucosal thickness was significantly greater on Day 8 than Day 28 p.i. (p=0.000) and in C than HFW lambs. There were fewer parietal cells on Day 28 than on Day 8 p.i. (p=0.003) for pooled data. Circulating eosinophil counts increased moderately in both groups, significantly less in the HFW lambs. Fewer tissue and blood eosinophils in the HFW than C group on Day 8 p.i. were consistent with cytokine gene expression patterns, particularly lower IL-5 levels. Worm count decreased by 90% by Day 28 p.i., along with declining tissue eosinophil counts and IL-13 gene expression and increasing IL-10 and IL-4 gene expression. Food intake was depressed less in the HFW lambs, suggesting that maintenance of appetite could be an important aspect of the physiological basis for resilience. Although the resilient phenotype was not apparent at the younger age, lesser effects on food intake, differences in ALN cytokine profiles and lower blood and tissue eosinophil numbers in the HFW lambs may lead to the expression of resilience when older.
Simpson
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HV
Institute of Veterinary, Animal & Biomedical Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand. H.V.Simpson@massey.ac.nz
Przemeck
S M C
SM
Scott
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I
Pernthaner
A
A
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004435:Eating / Q000276:immunology; D004804:Eosinophils / Q000166:cytology; D005243:Feces / Q000469:parasitology; D005786:Gene Expression Regulation; D006197:Hair; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D010028:Ostertagia / Q000502:physiology; D010029:Ostertagiasis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D010270:Parasite Egg Count; D012641:Selection, Genetic; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D013997:Time Factors
D016207:Cytokines