Elastic constants in tetragonal hen egg-white lysozyme crystals containing large amount of water.
Transverse sound velocity of cross-linked tetragonal hen egg-white (HEW) lysozyme crystals containing large amount of water in the crystal was measured using ultrasonic pulse-echo method. All elastic constants of cross-linked crystals were observed to be C11=C22=5.50 GPa, C12=4.33 GPa, C13=C23=3.94 GPa, C33=5.22 GPa, C44=C55=0.68 GPa, and C66=0.84 GPa, respectively. We found that the elastic constants of the cross-linked crystals are identical to those of the intrinsic ones without cross-linking. Moreover, we found that tetragonal HEW lysozyme crystals that enclose large amount of water show decreased elastic constants (softening). In particular, the shear elastic constants C44=C55 and C66 showed more softening effect comparing with other elastic components.
Koizumi
H
H
International Graduate School of Arts and Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
Tachibana
M
M
Kojima
K
K
D000042:Absorption; D000818:Animals; D002645:Chickens; D003198:Computer Simulation; D003460:Crystallization; D004548:Elasticity; D005260:Female; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry*; D013314:Stress, Mechanical; D014867:Water / Q000737:chemistry*
D014867:Water; C421864:hen egg lysozyme; D009113:Muramidase