title: Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy.
abstract: Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electrophoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.
authors: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors: Yusuke
authors: Y
authors: Sato
authors: Motoyuki
authors: M
authors: Shimizu
authors: Wataru
authors: W
authors: Mizunoya
authors: Hiroyuki
authors: H
authors: Wariishi
authors: Ryuichi
authors: R
authors: Tatsumi
authors: Vladimir L
authors: VL
authors: Buchman
authors: Yoshihide
authors: Y
authors: Ikeuchi
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D009121:Muscle Denervation; D009124:Muscle Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000294:innervation / Q000378:metabolism*; D009133:Muscular Atrophy / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009210:Myofibrils / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D009124:Muscle Proteins