title: Non-invasive diagnosis of early pulmonary disease in PECAM-deficient mice using infrared pulse oximetry.
abstract: Pulse oximetry is a common tool for detecting reduced pulmonary function in human interstitial lung diseases. It has not previously been used in a mouse model of interstitial lung disease. Further, platelet endothelial cell adhesion molecule deficient mice rarely show symptoms until disease is advanced. Using blood oxygen saturation, different stages of disease could be identified in a non-invasive manner. These stages could be correlated to pathology. Collagen deposition, using Picrosirius Red, did correlate with blood oxygen saturation. These studies are the first to show the use of an infrared pulse oximetry system to analyze the progression of a fibrotic interstitial lung disease in a mouse model of the human diseases. Further, these studies show that an early alveolar damage/enlargement event precedes the fibrosis in this mouse model, a stage that represents the best targets for disease analysis and prevention. This stage does not have extensive collagen deposition. Most importantly, targeting this earliest stage of disease for therapeutic intervention may lead to novel treatment for human disease.
authors: Department of Microbiology, Immunology & Pathology, Colorado State University, 1682 Campus Delivery Fort Collins, CO 80523-1682, USA.
authors: Merideth A
authors: MA
authors: Early
authors: Marta
authors: M
authors: Lishnevsky
authors: John M
authors: JM
authors: Gilchrist
authors: David M
authors: DM
authors: Higgins
authors: Ian M
authors: IM
authors: Orme
authors: William A
authors: WA
authors: Muller
authors: Mercedes
authors: M
authors: Gonzalez-Juarerro
authors: Alan R
authors: AR
authors: Schenkel
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D042241:Early Diagnosis; D007150:Immunohistochemistry; D007259:Infrared Rays; D017563:Lung Diseases, Interstitial / Q000175:diagnosis* / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D010092:Oximetry; D010100:Oxygen / Q000097:blood; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D010100:Oxygen