Enzyme-release capillary as a facile enzymatic biosensing part for a capillary-assembled microchip.
A simple capillary enzymatic biosensor was developed. This was prepared by simply coating a dissolvable membrane containing enzyme/s on the inner wall of a square glass capillary. An easy measurement was carried out by capillary force sample introduction with concurrent enzyme release and a reaction with a certain substrate. Enzyme-release capillary (ERC) biosensors showed long-term storage stability of at least two weeks for a beta-galactoside derivative and glucose. Moreover, this could be integrated on a capillary-assembled microchip (CAs-CHIP) to broaden its multiple analyte sensing potential for clinical diagnostic applications.
Henares
Terence G
TG
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Japan.
Maekawa
Eiji
E
Okubo
Fumito
F
Mizutani
Fumio
F
Yao
Toshio
T
Sekizawa
Ryuichi
R
Hisamoto
Hideaki
H
D015374:Biosensing Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D005697:Galactosides / Q000032:analysis*; D005947:Glucose; D056656:Lab-On-A-Chip Devices
D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D005697:Galactosides; C007816:beta-galactoside; D005947:Glucose