[Microfluidic cell culture array chip for drug screening assays].
We developed a novel microfluidic cell chip, which enabled drug delivery, fluid control and cell co-culture. The device consisted of an array of 6x6 cell culture chambers, a drug gradient generator and fluidic control valves. Micro-dam structures of the chambers were able to trap cells while loading and drug gradient network generated drug gradient of 6 different concentrations. Also we applied hydraulic valves to control the microfluid and simulate the microenvironment of cells. We had investigated the viability of co-culturing cells in the chip and the ability for drug screening. This microfluidic cell chip has the potential in cell-based research of high throughput drug screening.
Zheng
Yunhuan
Y
Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Wu
Jianzhang
J
Shao
Jianbo
J
Jin
Qinghui
Q
Zhao
Jianlong
J
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D014471:Umbilical Veins / Q000166:cytology