title: Rapid decay of host-specific fecal Bacteroidales cells in seawater as measured by quantitative PCR with propidium monoazide.
abstract: We investigated the persistence of feces-derived Bacteroidales cells and their DNA in seawater under natural conditions using an optimized chemical method based on co-extraction of nucleic acids with propidium monoazide (PMA), which interferes with PCR amplification of molecular markers from extracellular DNA and dead bacterial cells. The previously validated Bacteroidales assays BacUni-UCD, BacHum-UCD, BacCow-UCD, and BacCan-UCD were utilized to determine concentrations of Bacteroidales genetic markers targeting all warm-blooded animals, humans, cows and dogs, specifically, over a period of 24d. Microcosms containing mixed feces in dialysis tubing were exposed to seawater under flow-through conditions at ambient temperature in the presence and absence of sunlight. Using a two-stage plus linear decay model, the average T(99) (two-log reduction) of host-specific Bacteroidales cells was 28h, whereas that of host-specific Bacteroidales DNA was 177h. Natural sunlight did not affect the survival of uncultivable Bacteroidales cells and their DNA with the exception of the BacCow-UCD marker. Bacteroidales DNA, as measured by quantitative PCR (qPCR) without PMA, persisted for as long as 24d at concentrations close to the limit of detection. Culturable Enterococcus cells were detected for only 70h, whereas Enterococcus cells measured by qPCR with and without PMA persisted for 450h. In conclusion, measuring Bacteroidales DNA without differentiating between intact and dead cells or extracellular DNA may misinform about the extent of recent fecal pollution events, particularly in the case of multiple sources of contamination with variable temporal and spatial scales due to the relatively long persistence of DNA in the environment. In contrast, applying qPCR with and without PMA can provide data on the fate and transport of fecal Bacteroidales in water, and help implement management practices to protect recreational water quality.
authors: Department of Civil and Environmental Engineering, 2001 EU III, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors: Sungwoo
authors: S
authors: Bae
authors: Stefan
authors: S
authors: Wuertz
mesh_terms: D001386:Azides; D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods*; D016133:Polymerase Chain Reaction; D011419:Propidium / Q000031:analogs & derivatives; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D014873:Water Pollutants / Q000302:isolation & purification*
chemical_list: D001386:Azides; D012336:RNA, Ribosomal, 16S; D014873:Water Pollutants; C533957:propidium monoazide; D011419:Propidium