Self-resistance mechanism in Streptomyces peucetius: overexpression of drrA, drrB and drrC for doxorubicin enhancement.
The resistance genes drrABC from Streptomyces peucetius ATCC 27952 were cloned into the pIBR25 expression vector under a strong ermE* promoter to enhance doxorubicin (DXR) production. The recombinant expression plasmids, pDrrAB25, pDrrC25 and pDrrABC25, were constructed to overexpress drrAB, drrC and drrABC, respectively, in S. peucetius ATCC 27952. The recombinant strains produced more DXR than the parental strain: a 2.2-fold increase with pDrrAB25, a 5.1-fold increase with pDrrC25, and a 2.4-fold increase with pDrrABC25. We also studied the relative ratios of doxorubicin, daunorubicin and epsilon-rhodomycinone produced in these recombinant strains.
Malla
Sailesh
S
Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, SunMoon University, # 100 Kalsan-ri, Asansi, Chungnam 336-708, Republic of Korea.
Niraula
Narayan Prasad
NP
Liou
Kwangkyoung
K
Sohng
Jae Kyung
JK
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004317:Doxorubicin / Q000096:biosynthesis* / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D004317:Doxorubicin; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C104037:DrrA protein, Streptomyces peucetius; C104038:DrrB protein, Streptomyces peucetius