title: Backtracking and proofreading in DNA transcription.
abstract: Biological cell function crucially relies on the accuracy of RNA sequences, transcribed from the DNA genetic code. To ensure sufficiently high fidelity in the face of high spontaneous error rates during transcription, error correction mechanisms must play an important role. A particular mechanism of transcriptional error correction involves backtracking of the RNA polymerase and RNA cleavage. Motivated by recent single molecule experiments characterizing the dynamics of backtracking, we present a microscopic model of this editing process. We show that such a mechanism can yield error frequencies that are in agreement with in vivo observations.
authors: School of Computing, Department of Applied Mathematics, University of Leeds, Leeds, LS2 9JT, United Kingdom.
authors: Margaritis
authors: M
authors: Voliotis
authors: Netta
authors: N
authors: Cohen
authors: Carmen
authors: C
authors: Molina-París
authors: Tanniemola B
authors: TB
authors: Liverpool
mesh_terms: D004247:DNA / Q000235:genetics* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007700:Kinetics; D008957:Models, Genetic; D012313:RNA / Q000235:genetics / Q000378:metabolism; D013269:Stochastic Processes; D013816:Thermodynamics; D014158:Transcription, Genetic
chemical_list: D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases