title: Cis-regulatory changes at FLOWERING LOCUS T mediate natural variation in flowering responses of Arabidopsis thaliana.
abstract: Flowering time, a critical adaptive trait, is modulated by several environmental cues. These external signals converge on a small set of genes that in turn mediate the flowering response. Mutant analysis and subsequent molecular studies have revealed that one of these integrator genes, FLOWERING LOCUS T (FT), responds to photoperiod and temperature cues, two environmental parameters that greatly influence flowering time. As the central player in the transition to flowering, the protein coding sequence of FT and its function are highly conserved across species. Using QTL mapping with a new advanced intercross-recombinant inbred line (AI-RIL) population, we show that a QTL tightly linked to FT contributes to natural variation in the flowering response to the combined effects of photoperiod and ambient temperature. Using heterogeneous inbred families (HIF) and introgression lines, we fine map the QTL to a 6.7 kb fragment in the FT promoter. We confirm by quantitative complementation that FT has differential activity in the two parental strains. Further support for FT underlying the QTL comes from a new approach, quantitative knockdown with artificial microRNAs (amiRNAs). Consistent with the causal sequence polymorphism being in the promoter, we find that the QTL affects FT expression. Taken together, these results indicate that allelic variation at pathway integrator genes such as FT can underlie phenotypic variability and that this may be achieved through cis-regulatory changes.
authors: Plant Biology Laboratory, Howard Hughes Medical Institute, The Salk Institute for Biological Sciences, La Jolla, California 92037, USA.
authors: Christopher
authors: C
authors: Schwartz
authors: Sureshkumar
authors: S
authors: Balasubramanian
authors: Norman
authors: N
authors: Warthmann
authors: Todd P
authors: TP
authors: Michael
authors: Janne
authors: J
authors: Lempe
authors: Sridevi
authors: S
authors: Sureshkumar
authors: Yasushi
authors: Y
authors: Kobayashi
authors: Julin N
authors: JN
authors: Maloof
authors: Justin O
authors: JO
authors: Borevitz
authors: Joanne
authors: J
authors: Chory
authors: Detlef
authors: D
authors: Weigel
mesh_terms: D017360:Arabidopsis / Q000145:classification / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010641:Phenotype; D017440:Photoperiod; D011401:Promoter Regions, Genetic / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; C418905:FT protein, Arabidopsis