title: Crystallization and preliminary X-ray analysis of gamma-hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26.
abstract: LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from gamma-hexachlorocyclohexane (gamma-HCH) to 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN) via gamma-pentachlorocyclohexene (gamma-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P4(1) or P4(3), with unit-cell parameters a = b = 68.9, c = 101.9 A, and diffracted X-rays to 2.25 A resolution. The crystal contained three molecules in the asymmetric unit.
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors: Masahiko
authors: M
authors: Okai
authors: Keiko
authors: K
authors: Kubota
authors: Masao
authors: M
authors: Fukuda
authors: Yuji
authors: Y
authors: Nagata
authors: Koji
authors: K
authors: Nagata
authors: Masaru
authors: M
authors: Tanokura
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D008190:Lyases / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011487:Protein Conformation; D042301:Sphingomonadaceae / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D008190:Lyases