[Gene expression differences of toxigenic and nontoxigenic Vibrio cholerae strains in mannitol fermentation medium and Luria-Bertani broth].
OBJECTIVE
To analyze gene expression differences of toxigenic and nontoxigenic strains of El Tor Vibrio cholerae growing separately in mannitol fermentation medium and LB (Luria-Bertani) broth.


METHODS
Total RNA was extracted from the mannitol slow-fermenting strain N16961 (toxigenic) and the mannitol fast-fermenting strain 93097 (nontoxigenic) at 1 h of fermentation. The large scale gene expression profiles were detected and compared with high throughout microarray.


RESULTS
By comparing the strains growing in different cultures, we found 142 differentially expressed genes in N16961 and 418 genes in 93097. Most of these genes were grouped into six functional classes. They were mainly related to transport and binding, energy metabolism, protein biosynthesis, and protein fate.


CONCLUSIONS
The expression levels of genes in N16961 and 93097 were affected by culture conditions, which can serve as basis for further studying the mechanism of metabolism of mannitol.
Zhang
Hongzhi
H
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. wxws46@vip.sohu.net
Pang
Bo
B
Zhang
Li
L
Kan
Biao
B
D000818:Animals; D002771:Cholera / Q000235:genetics / Q000378:metabolism*; D017077:Culture Media, Conditioned; D005285:Fermentation; D049851:Gastropoda / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008353:Mannitol / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism
D017077:Culture Media, Conditioned; D008353:Mannitol