title: Antimicrobial activity of vanadium chloroperoxidase on planktonic Streptococcus mutans cells and Streptococcus mutans biofilms.
abstract: The aim of this study was to investigate the antimicrobial activity of vanadium chloroperoxidase (VCPO) reaction products on planktonic and biofilm cells of Streptococcus mutans C180-2. Planktonic and biofilm cells were incubated in a buffered reaction mixture containing VCPO, halide (either chloride or bromide) and hydrogen peroxide, and the killing efficacy was assessed by CFU counts. The enzymatic products formed by VCPO significantly reduced the viability of planktonic and biofilm cells compared to their negative controls and the effect on the biofilm cells was more effective than a 0.2% chlorhexidine digluconate treatment. We conclude that VCPO and its reaction products form a potent antimicrobial system against S. mutans.
authors: Department of Cariology, Endodontology, Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. m.hoogenkamp@acta.nl
authors: M A
authors: MA
authors: Hoogenkamp
authors: W
authors: W
authors: Crielaard
authors: J M
authors: JM
authors: ten Cate
authors: R
authors: R
authors: Wever
authors: A F
authors: AF
authors: Hartog
authors: R
authors: R
authors: Renirie
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002711:Chloride Peroxidase / Q000494:pharmacology*; D015169:Colony Count, Microbial; D010933:Plankton / Q000187:drug effects / Q000382:microbiology; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; C418625:vanadium chloroperoxidase; D002711:Chloride Peroxidase