Structure of the choline-binding domain of Spr1274 in Streptococcus pneumoniae.
Spr1274 is a putative choline-binding protein that is bound to the cell wall of Streptococcus pneumoniae through noncovalent interactions with the choline moieties of teichoic and lipoteichoic acids. Its function is still unknown. The crystal structure of the choline-binding domain of Spr1274 (residues 44-129) was solved at 2.38 A resolution with three molecules in the asymmetric unit. It may provide a structural basis for functional analysis of choline-binding proteins.
Zhang
Zhenyi
Z
Protein Research Institute, Tongji University, Shanghai 200092, People's Republic of China.
Li
Wenzhe
W
Frolet
Cecile
C
Bao
Rui
R
di Guilmi
Anne Marie
AM
Vernet
Thierry
T
Chen
Yuxing
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002794:Choline / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013296:Streptococcus pneumoniae / Q000378:metabolism*
D001426:Bacterial Proteins; D002794:Choline