Magic Angle Spinning (MAS) NMR: a new tool to study the spatial and electronic structure of photosynthetic complexes.
In the last two decades, Magic Angle Spinning (MAS) NMR has created its own niche in studies involving photosynthetic membrane protein complexes, owing to its ability to provide structural and functional information at atomic resolution of membrane proteins when in the membrane, in the natural environment. The light-harvesting two (LH2) transmembrane complex from Rhodopseudomonas acidophila is used to illustrate the procedure of the technique applicable in photosynthesis research. One- and two-dimensional solid-state NMR experiments involving (13)C- and (15)N-labeled LH2 complexes allow to make a sequence-specific assignment of NMR signals, which forms the basis for resolving structural details and the assessment of charge transfer, electronic delocalization effects, and functional strain in the ground state.
Alia
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Leiden Institute of Chemistry, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Ganapathy
Swapna
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de Groot
Huub J M
HJ
D000595:Amino Acid Sequence; D004583:Electrons; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D011522:Protons
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D011522:Protons