[Extraction and purification of Atractylodes macrocephala monosaccharide composition].
OBJECTIVE
To study the method of Atractylodes macrocephala monosaccharide composition (AMMC)'s extraction, purification and analysis.


METHODS
To extract AMMC with water extraction method and purify it with neutro-alumina and resin adsorption method, to observe removal impurity effect of several methods and determine the technology parameters. To detect monosaccharide's content in AMMC with hydrochloric acid-ultraviolet spectrophotometric determination and Evaporative light scattering detector (ELSD).


RESULTS
After purificated with neutro-alumina, the yield and purity of AMMC were 23% - 25.5% and 56% - 60%, respectively. According to the static decoloring curve of D900, D201 and AB-8 resins, their decolorization effect's rank was D900 > AB-8 > D201. The yield of AMMC purificated with D900 ion exchange resin was above 17%, and the purity of monosaccharide was above 76%.


CONCLUSIONS
The effect of AMMC purificated with D900 ion exchange resin after water extration is much better, the ideal method for the quantitative analysis of monosaccharide is hydrochloric acid-ultraviolet spectrophotometric determination.
Shi
Zhong-Feng
ZF
Piwei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Li
Ru-Liu
RL
Chen
Wei-Wen
WW
D000327:Adsorption; D000537:Aluminum Oxide; D000837:Anion Exchange Resins; D031174:Atractylodes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005632:Fructose / Q000032:analysis; D009005:Monosaccharides / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D027343:Rhizome / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water
D000837:Anion Exchange Resins; D009005:Monosaccharides; D012117:Resins, Synthetic; D014867:Water; D005632:Fructose; D000537:Aluminum Oxide