Successive bouts of cycling stimulates genes associated with mitochondrial biogenesis.
Exercise increases mRNA for genes involved in mitochondrial biogenesis and oxidative enzyme capacity. However, little is known about how these genes respond to consecutive bouts of prolonged exercise. We examined the effects of 3 h of intensive cycling performed on three consecutive days on the mRNA associated with mitochondrial biogenesis in trained human subjects. Forty trained cyclists were tested for VO(2max) (54.7 +/- 1.1 ml kg(-1) min(-1)). The subjects cycled at 57% watts(max) for 3 h using their own bicycles on CompuTrainer Pro Model trainers (RacerMate, Seattle, WA) on three consecutive days. Muscle biopsies were obtained from the vastus lateralis pre- and post-exercise on days one and three. Muscle samples were analyzed for mRNA content of peroxisome proliferator receptor gamma coactivator-1 alpha (PGC-1alpha), sirtuin 1 (Sirt-1), cytochrome c, and citrate synthase. Data were analyzed using a 2 (time) x 2 (day) repeated measures ANOVA. Of the mRNA analyzed, the following increased from pre to post 3 h rides: cytochrome c (P = 0.006), citrate synthase (P = 0.03), PGC-1alpha (P < 0.001), and Sirt-1 (P = 0.005). The following mRNA showed significant effects from days one to three: cytochrome c (P < 0.001) and citrate synthase (P = 0.01). These data show that exhaustive cycling performed on three consecutive days resulted in both acute and chronic stimuli for mRNA associated with mitochondrial biogenesis in already trained subjects. This is the first study to illustrate an increase in sirtuin-1 mRNA with acute and chronic exercise. These data contribute to the understanding of mRNA expression during both acute and successive bouts of prolonged exercise.
Dumke
Charles L
CL
Department of Health and Human Performance, University of Montana, Missoula, MT 59812, USA. charles.dumke@mso.umt.edu
Mark Davis
J
J
Angela Murphy
E
E
Nieman
David C
DC
Carmichael
Martin D
MD
Quindry
John C
JC
Travis Triplett
N
N
Utter
Alan C
AC
Gross Gowin
Sarah J
SJ
Henson
Dru A
DA
McAnulty
Steven R
SR
McAnulty
Lisa S
LS
D000328:Adult; D001642:Bicycling / Q000502:physiology*; D002950:Citrate (si)-Synthase / Q000235:genetics; D045304:Cytochromes c / Q000235:genetics; D015444:Exercise / Q000502:physiology; D050259:Genes, Mitochondrial; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics; D010507:Periodicity; D010807:Physical Endurance / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D056564:Sirtuin 1 / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D045304:Cytochromes c; D002950:Citrate (si)-Synthase; D056564:Sirtuin 1