title: Hematopoietic progenitor cells increase remarkably in the peripheral blood of children with infection.
abstract: BACKGROUND
  Peripheral blood (PB) of children with viral infection had remarkable increase in colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colonies and clusters. The authors sought to investigate the hematopoietic changes in children with infection.
  
  
  METHODS
  The CFU-GM-derived colonies and clusters, burst-forming unit-erythroid (BFU-E)-derived colonies, and colony-forming unit-megakaryocyte (CFU-MK)-derived colonies assays and methylcellulose and/or agarose culture of PB were performed in 25 controls and 42 infected patients. Hematopoietic cell assays and cell culture of bone marrow (BM) were performed in 28 controls and 12 infected patients.
  
  
  RESULTS
  The PB controls had very few CFU-GM-derived colonies and clusters. The PB of infected children had many more CFU-GM-derived colonies and clusters. In addition, PB of infected children had abundant BFU-E-derived colonies and CFU-MK-derived colonies, whereas, from BM there were no statistical differences in the numbers of CFU-GM-derived colonies and clusters, BFU-E-derived colonies, and CFU-MK-derived colonies between infection group and control group. The numbers of CFU-GM-derived colonies and clusters were greater in PB of bacteria-infected patients than those in PB of non-bacteria-infected patients.
  
  
  CONCLUSIONS
  PB of infected children had increased numbers of colonies, especially in the bacteria-infected group.
authors: Division of Pediatric Hematology-Oncology, Department of Pediatrics, Mackay Memorial Hospital, Taipei, Taiwan.
authors: Ting-Chi
authors: TC
authors: Yeh
authors: Lee-Yung
authors: LY
authors: Shih
authors: I-Jen
authors: IJ
authors: Chai
authors: Hsi-Che
authors: HC
authors: Liu
authors: Lin-Yen
authors: LY
authors: Wang
authors: Nan-Chang
authors: NC
authors: Chiu
authors: Shu-Huey
authors: SH
authors: Chen
authors: Hsin
authors: H
authors: Chi
authors: Der-Cherng
authors: DC
authors: Liang
mesh_terms: D000293:Adolescent; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology*; D006801:Humans; D007223:Infant; D007239:Infections / Q000097:blood*; D008297:Male; D055015:Megakaryocyte-Erythroid Progenitor Cells / Q000166:cytology*