Electrical signaling, stomatal conductance, ABA and ethylene content in avocado trees in response to root hypoxia.
Avocado (Persea americana Mill.) trees are among the most sensitive of fruit tree species to root hypoxia as a result of flooded or poorly drained soil. Similar to drought stress, an early physiological response to root hypoxia in avocado is a reduction of stomatal conductance. It has been previously determined in avocado trees that an extracellular electrical signal between the base of stem and leaves is produced and related to reductions in stomatal conductance in response to drought stress. The current study was designed to determine if changes in the extracellular electrical potential between the base of the stem and leaves in avocado trees could also be detected in response to short-term (min) or long-term (days) root hypoxia, and if these signals could be related to stomatal conductance (gs), root and leaf ABA and ACC concentrations, ethylene emission from leaves and leaf abscission. In contrast to previous observations for drought-stressed trees, short-term or long-term root hypoxia did not stimulate an electrical potential difference between the base of the stem and leaves. Short-term hypoxia did not result in a significant decrease in gs compared with plants in the control treatment, and no differences in ABA concentration were found between plants subjected to hypoxia and control plants. Long-term hypoxia in the root zone resulted in a significant decrease in gs, increased leaf ethylene and increased leaf abscission. The results indicate that for avocado trees exposed to root hypoxia, electrical signals do not appear to be the primary root-to-shoot communication mechanism involved in signaling for stomatal closure as a result of hypoxia in the root zone.
Gil
Pilar M
PM
Departamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Casilla, Santiago, Chile. pgil@inia.cl
Gurovich
Luis
L
Schaffer
Bruce
B
García
Nicolás
N
Iturriaga
Rodrigo
R
D000040:Abscisic Acid / Q000032:analysis*; D015687:Cell Hypoxia; D055864:Droughts; D055724:Electrophysiological Phenomena; D005030:Ethylenes / Q000032:analysis*; D027421:Persea / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology; D018517:Plant Roots / Q000502:physiology*; D018520:Plant Shoots / Q000502:physiology; D054046:Plant Stomata / Q000502:physiology*; D014197:Trees / Q000502:physiology
D005030:Ethylenes; D000040:Abscisic Acid; C036216:ethylene