title: Laboratory maintenance of Mycobacterium smegmatis.
abstract: This unit gives background information on Mycobacterium smegmatis, a mycobacterial model system, and covers all the laboratory maintenance for this species including growth in liquid and on solid medium. It also contains recommendations concerning long-term strain storage. Although M. smegmatis is a Biosafety Level 1 organism, some rare infections in humans have been reported, and, thus all of the required safety measures are discussed here.
authors: University of Paris Descartes, Paris, France.
authors: Anil Kumar
authors: AK
authors: Singh
authors: Jean-Marc
authors: JM
authors: Reyrat
mesh_terms: D003264:Containment of Biohazards; D003470:Culture Media / Q000032:analysis; D046508:Culture Techniques / Q000379:methods*; D005612:Freeze Drying / Q000379:methods; D020102:Mycobacterium smegmatis / Q000254:growth & development*
chemical_list: D003470:Culture Media