Identification of tumor stem-like cells in a mouse myeloma cell line.
We used colony formation assay in the soft agar media or the serum-free media, the methods of identifying BrdU-label-retaining cells and the SP cells as well as the tumorigenicity test in BALB/c mice, respectively, to analyze tumor stem like cells in the SP2/0 cell line. The results showed that a few SP2/0 cells were capable of forming colonies in the soft agar media, contained BrdU-label-immortal strand in the SP2/0 cell line. The SP2/0 cells in the serum-free media gained higher tumorigenicity in the BALB/c mice than the SP2/0 cells cultivated in the complete media did. Overall, only a few of the SP2/0 cells were found to possess the characteristics of tumor stem-like cells, such as high proliferative potency, more self-renewal and stronger tumorigenesis, or greater similarity to the tumor stem cells (TSCs) traits. The biology of tumor stem-like cells contributes to the identification of molecular targets important for future tumor therapy.
Dou
J
J
Southeast University Department of Pathogenic Biology and Immunology; School of Basic Medical Science, Nanjing, China. njdoujun@yahoo.com.cn
Li
Y
Y
Zhao
F
F
Hu
W
W
Wen
P
P
Tang
Q
Q
Chu
L
L
Wang
Y
Y
Cao
M
M
Jiang
C
C
Gu
N
N
D000818:Animals; D001973:Bromodeoxyuridine / Q000008:administration & dosage / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003470:Culture Media / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D047368:Tumor Burden; D014410:Tumor Stem Cell Assay / Q000379:methods*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D001973:Bromodeoxyuridine