[Local expression of tumor necrosis factor-alpha on premature rupture of membranes].
OBJECTIVE
to compare the expression of tumor necrosis factor-alpha (TNF-alpha) in ovular membranes with premature rupture (MPR) and with opportune rupture; to verify the association between the expression of the TNF-alpha in ovular membranes and the degree of chorioamnionitis, correlating the expression of the TNF-alpha and the membranes' time of rupture.


METHODS
ovular membranes from 31 parturients with MPR, with gestational ages over 34 weeks, and from parturients with opportune membranes' rupture, with gestational ages equal or over 37 weeks. Chorioamnionitis detection has been done by histopathological analysis. The evaluation of the TNF-alpha expression has been done by immune-histochemical technique, using the labile streptavidin-biotin-peroxidase (LSAB) method.


RESULTS
the average rupture time was 16.6 hours. The ratio of the TNF-alpha expression in the Control and Study Groups did not show a significant difference (chi(2)=6.6; p=0.08). In the Study Group, there was no correlation between the degree of chorioamnionitis and the intensity of TNF-alpha expression (Spearman's coefficient (Rs)=0.4; p=0.02).


CONCLUSIONS
there was no significant difference between the TNF-alpha expression in ovular membranes with premature or opportune rupture; in the Study Group, there was significant association between TNF-alpha expression and the degree of chorioamnionitis, and there was no association between rupture time and the intensity of TNF-alpha expression.
Roveran
Valquíria
V
Departamento de Obstetrícia e Ginecologia, Irmandade da Santa Casa de Misericórdia de São Paulo, São Paulo, SP, Brasil.
Silva
Maria Antonieta Longo Galvão
MA
Yamano
Lucia
L
Rodrigues
Lilian Paiva
LP
Vasquez
Mônica Lopez
ML
Piato
Sebastião
S
D002821:Chorioamnionitis / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism*; D006801:Humans; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D055815:Young Adult
D014409:Tumor Necrosis Factor-alpha