[Study on the enrichment and purification of total flavonoids in Microcos paniculata by macroporous adsorption resin].
OBJECTIVE
To establish an optimum enrichment and purification process of total flavonoids in Microcos paniculata by macroporous resins.


METHODS
Five kinds of resins were compared and the best one was chosen. Then the parameters of the process were optimized by single factor tests, uniform design and statistical methods.


RESULTS
DI01 was selected for its excellent adsorption and desorption properties, 70% ethanol was found to be the best elution solution. As far as the yield was considered, the best result was based on the followings: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-7.88 mg/mL, eluting agent amount-2.0 BV, pH value 4.8; then the yield reached 90.18% and the purity was 54.37%. If the purity was considered, the best parameters wereas follows: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-2.0 mg/mL, eluting agent amount-2.8 BV, pH value 7.8; then the purity reached 61.77% and the yield was 80.25%.


CONCLUSIONS
The total flavonoids of Microcos paniculata can be effectively purificated and separated by D101 macroporous resin.
Li
Kun-Ping
KP
Guangdong Pharmaceutical University, Guangzhou 510006, China.
Pan
Tian-Ling
TL
Gao
Chong-Kai
CK
Li
Wei-Min
WM
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D029646:Tiliaceae / Q000737:chemistry*
D005419:Flavonoids; D012117:Resins, Synthetic; D000431:Ethanol