Spectroelectrochemical characterization of pain biomarkers.
This article reports the first electrochemical characterization of pain biomarkers that include arachidonic acid (AA), prostaglandin G(2) (PGG(2)), and cyclooxygenase 2 (COX-2). These biomarkers are mediators of pathophysiology of pain, inflammation, and cell proliferation in cancer. The article also reports the development of an electrochemical immunosensor for monitoring these pain biomarkers. The results revealed that direct electron transfer between AA metabolites and the electrode could be easily monitored and that an enzyme-modified electrode dramatically enhanced bioelectrocatalytic activity toward AA. Cyclic voltammetric analysis of AA revealed a concentration-dependent anodic current with a slope of 2.37 and a limit of detection (LOD) of 0.25nM. This unique AA/gold electrode electron transfer provides a good electrochemical sensing platform for prostaglandin H(2) (PGH(2)) as the basis for quantitation of pain. An amperometric signal intensity of a COX-2 antibody-modified gold electrode was linear with COX-2 concentration in the range of 0.1-0.5microg/ml and an LOD of 0.095microg/ml. The results also revealed a linear correlation of the concentration of PGG(2) with an LOD of 0.227microM.
Omole
Marcells A
MA
Department of Chemistry, Center for Advanced Sensors and Environmental Monitoring, State University of New York at Binghamton, 13902, USA.
Noah
Naumih
N
Zhou
Lisa
L
Almaletti
Anas
A
Sadik
Omowunmi A
OA
Asemota
Helen N
HN
William
Elvira S
ES
Gilchrist
Jason
J
D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000032:analysis*; D055664:Electrochemical Techniques; D006801:Humans; D055767:Immobilized Proteins; D007124:Immunoenzyme Techniques; D007249:Inflammation; D008839:Microelectrodes; D010147:Pain Measurement / Q000379:methods; D011462:Prostaglandins G / Q000032:analysis*; D013053:Spectrophotometry
D015415:Biomarkers; D055767:Immobilized Proteins; D011462:Prostaglandins G; D016718:Arachidonic Acid; C038291:prostaglandin G2; D051546:Cyclooxygenase 2