title: The adenine nucleotide translocator 1 acts as a type 2 transglutaminase substrate: implications for mitochondrial-dependent apoptosis.
abstract: In this study we provide in vitro and in vivo evidence showing that the protein disulphide isomerase (PDI) activity of type 2 transglutaminase (TG2) regulates the correct assembly and function of the mitochondrial ADP/ATP transporter adenine nucleotide translocator 1 (ANT1). We demonstrate, by means of biochemical and morphological analyses, that ANT1 and TG2 physically interact in the mitochondria. Under physiological conditions, TG2's PDI activity regulates the ADP/ATP transporter function by controlling the oligomerization of ANT1. In fact, mitochondria isolated from hearts of TG2(-/-) mice exhibit increased polymerization of ANT1, paralleled by an enhanced ADP/ATP carrier activity, as compared to mitochondria belonging to TG2(+/+) mice. Interestingly, upon cell-death induction, ANT1 becomes a substrate for TG2's cross-linking activity and the lack of TG2 results in a reduction of apoptosis as well as in a marked sensitivity to the ADP/ATP exchange inhibition by atractyloside. These findings suggest a complex TG2-dependent regulation of the ADP/ATP transporter and reveal new important avenues for its potential applications in the treatment of some mitochondrial-dependent diseases, including cardiovascular and neurodegenerative diseases.
authors: Department of Therapeutic Research and Medicine Evaluation, National Institute of Health, Rome, Italy.
authors: W
authors: W
authors: Malorni
authors: M G
authors: MG
authors: Farrace
authors: P
authors: P
authors: Matarrese
authors: A
authors: A
authors: Tinari
authors: L
authors: L
authors: Ciarlo
authors: P
authors: P
authors: Mousavi-Shafaei
authors: M
authors: M
authors: D'Eletto
authors: G
authors: G
authors: Di Giacomo
authors: G
authors: G
authors: Melino
authors: L
authors: L
authors: Palmieri
authors: C
authors: C
authors: Rodolfo
authors: M
authors: M
authors: Piacentini
mesh_terms: D033741:Adenine Nucleotide Translocator 1 / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008929:Mitochondria, Heart / Q000378:metabolism*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D033741:Adenine Nucleotide Translocator 1; D051028:bcl-2-Associated X Protein; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins