Effect of supplemental Bacillus cultures on rumen fermentation and milk yield in Chinese Holstein cows.
Two experiments were conducted to study the effect of supplemental 100 g/day of live Bacillus cultures (2 x 10(11) cell of Bacillus subtilis and Bacillus licheniformis) on rumen fermentation as well as milk yield and composition in Chinese Holstein cows. In experiment 1, investigating 3 x 10 cows, milk yield and milk protein were increased by using B. licheniformis (p < 0.05) in comparison with an unsupplemented group and the B. subtilis group. Body weight was not significantly affected by Bacillus culture supplementation (p > 0.05). Percentage of milk fat and lactose was not significantly different between treatments (p > 0.05). But milk protein increased with B. licheniformis supplementation (p < 0.05). In experiment 2, carried out with three non-lactating ruminally and duodenally fistulated cows, results showed that B. licheniformis supplementation increased microbial crude protein flow into duodenum (p < 0.05) and decreased the ammonia nitrogen concentration in ruminal fluid at 0.5 h, 1 h, 3 h, 6 h after morning feeding (p < 0.05). Bacillus licheniformis supplementation increased total VFA and acetate concentration in ruminal fluid at 0.5 h, 1 h, 3 h, 6 h after morning feeding (p < 0.05). Bacillus subtilis had no significant effect on rumen fermentation characteristics, duodenal microbial N flow and ruminal apparent nutrient digestibility (p > 0.05). Bacillus licheniformis increased ruminal apparent nutrient digestibility of neutral detergent fibre, acid detergent fibre, and organic matter (p < 0.05).
Qiao
G H
GH
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, China.
Shan
A S
AS
Ma
N
N
Ma
Q Q
QQ
Sun
Z W
ZW
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology; D000818:Animals; D001407:Bacillus / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D002417:Cattle / Q000382:microbiology / Q000502:physiology*; D004063:Digestion; D005260:Female; D005285:Fermentation; D007774:Lactation; D008892:Milk / Q000737:chemistry / Q000378:metabolism*; D008894:Milk Proteins / Q000032:analysis; D009584:Nitrogen / Q000378:metabolism; D019936:Probiotics; D012417:Rumen / Q000378:metabolism / Q000382:microbiology*
D001426:Bacterial Proteins; D008894:Milk Proteins; D009584:Nitrogen