LDL oxidation, antioxidant capacity and growth of cultured grey mullet ( Mugil cephalus ) fed dietary sorghum distillery residue pretreated with polyethylene glycol.
Dietary sorghum distillery residue (SDR) showed antioxidant and blood thinning effects on grey mullet during winter, but inhibited their growth. The objective of this study was to establish a preliminary treatment of the dietary SDR with polyethylene glycol (PEG), a tannin-binding agent, to enhance growth and blood antioxidant capacity of grey mullet ( Mugil cephalus ) feed. The feeding trial was carried out from June to November. The water temperature was between 25 and 30 degrees C; the specific growth rate of mullet was reduced significantly by feeding diet containing 20% SDR in comparison to fish fed the control diet or diet containing 20% SDR and PEG. In the period of October-November, the water temperature decreased to 19-25 degrees C; the specific growth rates of the 20% SDR-PEG group and the 20% SDR group were 0.13 and 0.19% day(-1), respectively, significantly higher than those fed the control diet (0.07% day(-1)). Feeding with 20% SDR or 20% SDR-PEG diets resulted in prolonged lag phase of low-density lipoprotein (LDL) oxidation compared to fish fed the control diet. The total antioxidant capacity of the plasma of the grey mullet fed 20% SDR-PEG was 1.24 mmol/L, significantly higher than those in the fish fed 20% SDR diet (0.84 mmol/L) or the control (0.72 mmol/L). In vivo observations found that preliminary treatment of SDR with PEG eliminated the endogenous undesirable growth inhibitory factors but maintained its protective effects against LDL oxidation in blood and improved the total antioxidant capacity and cold adaptation of grey mullet. The ethanol extract of SDR contained 31.9 +/- 7.8 mg/g gallic acids equivalent. The concentration needed to scavenge 50% of the DPPH radicals (IC(50)) was 0.86 mg/mL. Increased gallic acid equivalent and decreased IC(50) of DPPH scavenging activity of SDR fed to fish increased the total antioxidant capacity in blood plasma of grey mullet significantly.
Lee
Shin Mei
SM
Department of Food Science, National Kinmen Institute of Technology, Kinmen, Taiwan, Republic of China.
Cheng
Hui Ling
HL
Pan
Bonnie Sun
BS
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D004032:Diet; D007220:Industrial Waste; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood*; D011092:Polyethylene Glycols; D012621:Seasons; D023701:Smegmamorpha / Q000097:blood / Q000254:growth & development*; D045868:Sorghum / Q000737:chemistry*; D013696:Temperature
D000975:Antioxidants; D007220:Industrial Waste; D008077:Lipoproteins, LDL; D011092:Polyethylene Glycols