title: [Effects of fusion gene of ubiquitin and porcine reproductive and respiratory syndrome virus M gene on the immune response in inoculated mice].
abstract: OBJECTIVE
  To evaluate the effects of the fusion gene of ubiquitin (Ub) and Porcine Reproductive and Respiratory Syndrome virus (PRRSV) M gene on the immune response in inoculated mice.
  
  
  METHODS
  Mouse Ub gene and PRRSV M gene were amplified by RT-PCR from BALB/c mice spleen cells and PRRSV Ch-1a strain, respectively, and the M and Ub gene (U-M) was fused by SOE PCR. Therefore, pVAX1-U-M and pVAX1-M recombinant plasmid were constructed for eukaryotic expression.
  
  
  RESULTS
  The fusion U-M and M protein expressions were verified in transfected BHK-21 cells by indirect fluorescence assay. Furthermore, both pVAX1-M and pVAX1-U-M induced specific humoral and cellar immune responses against PRRSV in the recombinant plasmid injected mice. However, pVAX1-U-M was able to induce higher level of T cell response then that of pVAX1-M (P<0.05), but lower level of antibody (P<0.05).
  
  
  CONCLUSIONS
  Expression of U-M fusion gene had ability to enhance specific T cell response against PRRSV, but no effect on stimulation of humoral response in inoculated mice.
authors: State Key Laboratory of Veterinary Biotechnology, The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. zwj-216@163.com
authors: Weijun
authors: W
authors: Zhang
authors: Tiegang
authors: T
authors: Tong
authors: Yu
authors: Y
authors: Bai
authors: Qun
authors: Q
authors: Wang
authors: Shulan
authors: S
authors: Xu
authors: Qingge
authors: Q
authors: Sun
authors: Ye
authors: Y
authors: Tian
authors: Tao
authors: T
authors: Yang
authors: Guangzhi
authors: G
authors: Tong
authors: Donglai
authors: D
authors: Wu
mesh_terms: D000818:Animals; D015870:Gene Expression; D050939:Gene Fusion / Q000276:immunology*; D055633:Immune System Phenomena / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000494:pharmacology*; D013552:Swine; D014162:Transfection; D025801:Ubiquitin / Q000235:genetics* / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage
chemical_list: D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; D014763:Viral Matrix Proteins; D014765:Viral Vaccines