[Repairing of peri-implant bone defect with platelet-rich plasma and platelet-rich plasma/ osteoinduction active material composite: an experimental study in dogs].
OBJECTIVE
To investigate the effect of platelet-rich plasma (PRP) and osteoinduction active material(OAM) compounded with PRP in the repair of bone defects of peri-implant and evaluate the feasibility of PRP as a material for repair of peri-implant bone defects.


METHODS
Four Beagle dogs underwent extraction of the mandibular first, second and fourth premolars to create edentulous regions. Three months later, titanium implants were inserted and bone defects around implants were created. The bone defects were repaired with PRP/OAM compounds in experimental group A, repaired with PRP/ tricalcium phosphate in experimental group B, and repaired with tricalcium phosphate in the control group. The animals were sacrificed at the end of 8-week and 16-week, respectively. The specimens were observed with histological examination and energy disperse analysis of Ca(2+). The data were analyzed with SPSS13.0 software package for one-way ANOVA.


RESULTS
At 8-week, in experimental group A, some parts of implants were directly contacted with new bone. Some new bones formed in the experimental group B. There was fibrous connection between the bone and implant in the control group. At 16-week, there was mature Haversian system in the experimental group A. There was good osseointegration between the bone and implant in the experimental groups but only was fibrous connection in the control group. At 8-and 16-week, there was significant difference between there groups in the percentage of Ca(2+) (P<0.05).


CONCLUSIONS
PRP and PRP/OAM composite can effectively accelerate the regeneration of peri-implant bone defects and improve the osseointegration in the interface between the implant and bone.
Zhao
Wang
W
Department of Stomatology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China. happyzhao4@sina.com
Wang
Qiu-xu
QX
Liu
Wei-xian
WX
D000818:Animals; D001861:Bone Regeneration; D002130:Calcium Phosphates; D004285:Dogs; D008334:Mandible; D016348:Osseointegration; D053657:Platelet-Rich Plasma; D014025:Titanium; D014945:Wound Healing
D002130:Calcium Phosphates; D014025:Titanium; C018392:tricalcium phosphate