title: Quassinoid constituents of Quassia amara L. leaf herbal tea. Impact on its antimalarial activity and cytotoxicity.
abstract: OBJECTIVE
  Our objective was to assess whether it could be contemplated to recommend Quassia amara young leaf tea for treatment against malaria, and if yes, set up a standard protocol for preparing the herbal tea.
  
  
  METHODS
  The leaf tea was extracted with methylene chloride and the organic extract was fractionated with HPLC. Pure compounds were characterized and their in vitro cytotoxicity and antiplasmodial activity was determined.
  
  
  CONCLUSIONS
  We discovered that antimalarial Quassia amara young leaf tea contains several quassinoids: simalikalactone D (SkD, 1), picrasin B (2), picrasin H (3), neoquassin (4), quassin (5), picrasin I (6) and picrasin J (7). These last two compounds are new. In addition, our experiments demonstrate that both biological activity and cytotoxicity of the remedy may be attributed solely to the presence of SkD.
  
  
  CONCLUSIONS
  In conclusion, this preparation should not be recommended for treatment of malaria until a clinical study in humans is performed with SkD.
authors: CNRS, UMR Ecofog, Université des Antilles et de la Guyane, Cayenne, France.
authors: Emeline
authors: E
authors: Houël
authors: Stéphane
authors: S
authors: Bertani
authors: Geneviève
authors: G
authors: Bourdy
authors: Eric
authors: E
authors: Deharo
authors: Valérie
authors: V
authors: Jullian
authors: Alexis
authors: A
authors: Valentin
authors: Séverine
authors: S
authors: Chevalley
authors: Didier
authors: D
authors: Stien
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D001628:Beverages / Q000592:standards*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D016488:Medicine, African Traditional; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects*; D032271:Quassia / Q000737:chemistry*; D036702:Quassins / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D010936:Plant Extracts; D036702:Quassins