title: Pretreatment with arginine preserves intestinal barrier integrity and reduces bacterial translocation in mice.
abstract: OBJECTIVE
  To evaluate the effects of arginine on intestinal barrier integrity and bacterial translocation (BT) in mice undergoing intestinal obstruction.
  
  
  METHODS
  Mice were divided into 3 groups, treated for 7 d before surgical intervention with isocaloric and isoprotein diets. The ARG group received a diet containing 2% arginine, the IO (intestinal obstruction) and Sham groups, standard chow diet. On the eighth day of treatment, all animals received diethylenetriamine pentaacetic acid (DTPA) solution labeled with 99mTechnetium (99mTc-DTPA) by gavage for intestinal permeability analysis. After 90 min, the animals were anesthetized and the terminal ileum ligated. The Sham group only underwent laparotomy. After 4, 8, and 18 h, blood was collected for radioactivity determination. Samples of ileum were collected 18 h after surgery for histological analysis. In another set of animals, BT was evaluated. After 7 d of treatment, all animals received 10(8) CFU/mL of 99mTc-E.coli by gavage; 90 min later they were submitted to the surgical procedure described above. BT was determined by the uptake of 99mTc-E.coli in blood, mesenteric lymph nodes, liver, spleen, and lungs, assessed 18 h after the surgery.
  
  
  RESULTS
  The intestinal permeability and BT were higher in the IO group when compared with the Sham group (P < 0.05). Arginine supplementation reduced intestinal permeability and BT to physiologic levels. Histological analysis showed mucosal ileum preservation in animals treated with arginine.
  
  
  CONCLUSIONS
  Arginine was able to preserve barrier integrity, thus reducing BT.
authors: Programa de Pós Graduação em Ciência de Alimentos, Faculdade de Farmácia; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. mirellenut@yahoo.com.br
authors: Mirelle L
authors: ML
authors: Viana
authors: Rosana G C
authors: RG
authors: Santos
authors: Simone V
authors: SV
authors: Generoso
authors: Rosa M E
authors: RM
authors: Arantes
authors: Maria Isabel T D
authors: MI
authors: Correia
authors: Valbert N
authors: VN
authors: Cardoso
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000494:pharmacology*; D018988:Bacterial Translocation / Q000187:drug effects*; D001769:Blood / Q000187:drug effects / Q000382:microbiology; D004032:Diet; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000517:prevention & control; D007082:Ileum / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D007415:Intestinal Obstruction; D007554:Isotopes; D008099:Liver / Q000187:drug effects / Q000382:microbiology; D008168:Lung / Q000187:drug effects / Q000382:microbiology; D008198:Lymph Nodes / Q000187:drug effects / Q000382:microbiology; D051379:Mice; D004369:Pentetic Acid; D010539:Permeability; D013154:Spleen / Q000187:drug effects / Q000382:microbiology; D013667:Technetium
chemical_list: D007554:Isotopes; D013667:Technetium; D004369:Pentetic Acid; D001120:Arginine