Ribosome display and selection of human anti-placental growth factor scFv derived from ovarian cancer patients.
Ribosome display is a powerful cell-free technology to select a desired antibody together with its encoding mRNA. In this study, a human single-chain variable fragment (scFv) library was generated from the peripheral blood lymphocyte RNA of 10 ovarian cancer patients and then panned against bead-conjugated human PlGF, a protein that may contribute to the growth and metastasis of ovarian cancer. A selected scFv antibody was evaluated by Western blot and its affinity constant was determined by noncompetitive enzyme immunoassay. This study highlights the ribosomal display technology for the selection of human antibody from patient-derived gene pools.
Li
Fang
F
Department of Obstetrics and Gynecology, Affiliated Tenth Hospital, Tongji University, Shanghai, 200072, People's Republic of China. 09lifang@tongji.edu.cn
Su
Pingping
P
Lin
Chao
C
Li
Hong
H
Cheng
Jiajing
J
Shi
Donglu
D
D015153:Blotting, Western; D004587:Electrophoresis, Agar Gel; D005260:Female; D015723:Gene Library; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D012270:Ribosomes; D057127:Single-Chain Antibodies / Q000235:genetics / Q000378:metabolism*
C000608036:PGF protein, human; D011257:Pregnancy Proteins; D057127:Single-Chain Antibodies; D000072483:Placenta Growth Factor