Establishment of differential expression profiles from invasive and non-invasive pituitary adenomas.
OBJECTIVE
To establish high resolution, reproducible 2-dimensional electrophoresis (2-DE) profiles of invasive and non-invasive pituitary adenoma tissues and to identify differentially expressed proteins between the invasive and non-invasive tissues.


METHODS
The proteome from invasive and non-invasive pituitary adenomas tissues was dissected and analyzed by: (1) immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis, (2) silver staining, (3) imageMaster 2-D software analysis, (4) peptide mass fingerprint based (PMS) on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and (5) database comparison.


RESULTS
High-resolution 2-D patterns of invasive and non-invasive pituitary adenoma tissues were successfully produced and repeated 3 times for each sample. An average of 1 080+/-24 and 1 035+/-28 spots were detected for invasive and non-invasive pituitary adenoma tissues, respectively. Additionally, 975+/-45 and 918+/-56 spots were found to have an average matching rate of 90.3% and 88.7% for invasive and non-invasive tissues, respectively. The spot positional deviation was (1.563+/-0.259) mm for IEF and (1.088+/-0.206) mm for SDS-PAGE. A total of 99 spots of differential expression were matched between the invasive and non-invasive pituitary adenoma tissues. Thirty differential proteins, some of which were involved in the regulation of cells cycle and signal transduction, were initially characterized by PMS.


CONCLUSIONS
The acquisition of well-resolved and reproducible 2-D patterns of invasive and non-invasive pituitary adenoma tissues and the identification of differentially expressed proteins provides a proteome database for invasive pituitary adenomas.
Liu
Zhixiong
Z
Department of Neurosurgery, Central South University, Changsha 410008, China.
Liu
Yunsheng
Y
Fang
Wenhua
W
Chen
Wei
W
Li
Cui
C
Xiao
Zhiqiang
Z
D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006801:Humans; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis*; D010911:Pituitary Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020543:Proteome / Q000032:analysis*
D009363:Neoplasm Proteins; D020543:Proteome