Wandonia haliotis gen. nov., sp. nov., a marine bacterium of the family Cryomorphaceae, phylum Bacteroidetes.
A novel, strictly aerobic, Gram-stain-negative, yellow-orange-pigmented, rod-shaped bacterium was isolated from abalone (Haliotis discus) under aquaculture in seawater off the Wando coast, Southern Korea, and subjected to a polyphasic taxonomic study. Cells of strain Haldis-1(T) were catalase- and oxidase-positive rods with flexirubin pigments. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Haldis-1(T) formed a distinct lineage within the family Cryomorphaceae and could be distinguished from the related genera Lishizhenia and Fluviicola. Strain Haldis-1(T) shared 16S rRNA gene sequence similarities of 92.5 and 92.4 % with Lishizhenia caseinilytica UST040201-001(T) and Fluviicola taffensis RW262(T), respectively. The DNA G+C content was 38.1 mol% and the major respiratory quinone was MK-7. The predominant cellular fatty acids were iso-C(15 : 0) (38.6 %), C(15 : 0) 2-OH (20.3 %) and C(15 : 0) (10.7 %). Growth was observed at 25-42 degrees C (optimum 30-37 degrees C) and at pH 6.5-9.5 (optimum pH 6.5-8.0). On the basis of polyphasic analysis of phenotypic, genotypic and phylogenetic data, strain Haldis-1(T) represents a novel genus and species within the family Cryomorphaceae in the phylum Bacteroidetes, for which the name Wandonia haliotis gen. nov., sp. nov. is proposed. The type strain is Haldis-1(T) (=KCTC 22610(T) =NBRC 105642(T)).
Lee
Dong-Heon
DH
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Choi
Eun-Kyoung
EK
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Moon
Sung-Ran
SR
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Ahn
Samyoung
S
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Lee
Young Sun
YS
Department of Life Science, Sunchon National University, Sunchon 540-742, Republic of Korea.
Jung
Jae Sung
JS
Department of Life Science, Sunchon National University, Sunchon 540-742, Republic of Korea.
Jeon
Che Ok
CO
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Whang
Kyung-Sook
KS
Division of Bio and Health Science, Mokwon University, Daejeon 302-729, Republic of Korea.
Kahng
Hyung-Yeel
HY
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
D000818:Animals; D017756:Aquaculture; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D049851:Gastropoda / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S