Electrical contacting of redox enzymes by means of oligoaniline-cross-linked enzyme/carbon nanotube composites.
The electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and thioaniline-modified glucose oxidase (GOx) on a thioaniline monolayer-modified Au electrode yields a CNTs/GOx composite cross-linked by the redox-active bis-aniline units. The three-dimensional conductivity of the CNTs matrix and the electron-transfer (relay) properties of the bis-aniline components lead to the effective electrical wiring of the enzyme units with the electrode, and to the efficient bioelectrocatalytic oxidation of glucose (turnover rate ca. k(et) = 1025 s(-1)). The conditions to synthesize the three-dimensional CNTs/GOx composite and the parameters influencing the bioelectrocatalytic functions of the modified electrode are discussed.
Baravik
Ilina
I
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Tel-Vered
Ran
R
Ovits
Oded
O
Willner
Itamar
I
D000814:Aniline Compounds / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction
D000814:Aniline Compounds; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D005949:Glucose Oxidase; C023650:aniline