title: Unique features of HLA-mediated HIV evolution in a Mexican cohort: a comparative study.
abstract: BACKGROUND
  Mounting evidence indicates that HLA-mediated HIV evolution follows highly stereotypic pathways that result in HLA-associated footprints in HIV at the population level. However, it is not known whether characteristic HLA frequency distributions in different populations have resulted in additional unique footprints.
  
  
  METHODS
  The phylogenetic dependency network model was applied to assess HLA-mediated evolution in datasets of HIV pol sequences from free plasma viruses and peripheral blood mononuclear cell (PBMC)-integrated proviruses in an immunogenetically unique cohort of Mexican individuals. Our data were compared with data from the IHAC cohort, a large multi-center cohort of individuals from Canada, Australia and the USA.
  
  
  RESULTS
  Forty three different HLA-HIV codon associations representing 30 HLA-HIV codon pairs were observed in the Mexican cohort (q < 0.2). Strikingly, 23 (53%) of these associations differed from those observed in the well-powered IHAC cohort, strongly suggesting the existence of unique characteristics in HLA-mediated HIV evolution in the Mexican cohort. Furthermore, 17 of the 23 novel associations involved HLA alleles whose frequencies were not significantly different from those in IHAC, suggesting that their detection was not due to increased statistical power but to differences in patterns of epitope targeting. Interestingly, the consensus differed in four positions between the two cohorts and three of these positions could be explained by HLA-associated selection. Additionally, different HLA-HIV codon associations were seen when comparing HLA-mediated selection in plasma viruses and PBMC archived proviruses at the population level, with a significantly lower number of associations in the proviral dataset.
  
  
  CONCLUSIONS
  Our data support universal HLA-mediated HIV evolution at the population level, resulting in detectable HLA-associated footprints in the circulating virus. However, it also strongly suggests that unique genetic backgrounds in different HIV-infected populations may influence HIV evolution in a particular direction as particular HLA-HIV codon associations are determined by specific HLA frequency distributions. Our analysis also suggests a dynamic HLA-associated evolution in HIV with fewer HLA-HIV codon associations observed in the proviral compartment, which is likely enriched in early archived HIV sequences, compared to the plasma virus compartment. These results highlight the importance of comparative HIV evolutionary studies in immunologically different populations worldwide.
authors: Center for Research in Infectious Diseases, National Institute of Respiratory Diseases, Mexico City, Mexico. santiago.avila@cieni.org.mx
authors: Santiago
authors: S
authors: Avila-Rios
authors: Christopher E
authors: CE
authors: Ormsby
authors: Jonathan M
authors: JM
authors: Carlson
authors: Humberto
authors: H
authors: Valenzuela-Ponce
authors: Juan
authors: J
authors: Blanco-Heredia
authors: Daniela
authors: D
authors: Garrido-Rodriguez
authors: Claudia
authors: C
authors: Garcia-Morales
authors: David
authors: D
authors: Heckerman
authors: Zabrina L
authors: ZL
authors: Brumme
authors: Simon
authors: S
authors: Mallal
authors: Mina
authors: M
authors: John
authors: Enrique
authors: E
authors: Espinosa
authors: Gustavo
authors: G
authors: Reyes-Teran
mesh_terms: D000220:Adaptation, Biological; D015331:Cohort Studies; D019143:Evolution, Molecular; D005260:Female; D005787:Gene Frequency; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D016333:HIV Protease / Q000235:genetics*; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D012641:Selection, Genetic
chemical_list: D006680:HLA Antigens; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1