title: Antioxidant activities of flavonol derivatives from the leaves and stem bark of Scutia buxifolia Reiss.
abstract: This study evaluated the antioxidant activities in the leaves and stem bark fractions of Scutia buxifolia. Cerebral lipid peroxidation (TBARS) was induced by Fe(II) and radical-scavenging activity was determined by DPPH method. Folin-Ciocalteu was used to determine phenolic contents. Quercetin, quercitrin, isoquercitrin and rutin were isolated from leaf ethyl acetate fraction and their levels were measured by high performance liquid chromatography-photodiode array detector. IC(50) (DPPH) varied from 4.35+/-1.30 to 29.55+/-0.54 microg/mL for stem bark and from 6.50+/-0.40 to 30.54+/-1.14 in the leaves. Ethyl acetate and butanolic fractions caused a sharp fall in TBARS production with IC(50) from 2.93+/-2.17 to 40.46+/-2.51 microg/mL for the leaves and 0.66+/-0.17 to 27.3+/-1.23 for the stem bark. Results obtained indicated that S. buxifolia has a great potential to prevent disease caused by the overproduction of free radicals and also it might be used as a potential source of natural antioxidant agents.
authors: Departamento de Farmácia Industrial, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors: Aline Augusti
authors: AA
authors: Boligon
authors: Romaiana Picada
authors: RP
authors: Pereira
authors: Andriéli Cassel
authors: AC
authors: Feltrin
authors: Michel Mansur
authors: MM
authors: Machado
authors: Vanessa
authors: V
authors: Janovik
authors: João Batista Teixeira
authors: JB
authors: Rocha
authors: Margareth Linde
authors: ML
authors: Athayde
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects; D010636:Phenols / Q000378:metabolism; D010851:Picrates / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D051381:Rats; D027823:Rhamnaceae / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D044948:Flavonols; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron