High-resolution genome-wide array comparative genomic hybridization in splenic marginal zone B-cell lymphoma.
Splenic marginal zone B-cell lymphoma is characterized by high genetic heterogeneity, and hepatitis C virus infection seems to be involved in a subset of patients. The aims of the analysis were to identify potential genetic alterations related to hepatitis C virus status, IgV(H) gene mutational status, and prognostic categories identified in a multicenter study (Blood 2006;107:4643). Genome-wide array comparative genomic hybridization at a 100-kilobase (kb) resolution was performed in 34 patients with splenic marginal zone B-cell lymphoma, 12 of whom were hepatitis C virus positive. Array-comparative genomic hybridization experiments revealed no copy number alterations in 10 patients (4 were hepatitis C virus positive). A median of 5.6 and 3.8 copy number alterations were detected in hepatitis C virus-positive and in hepatitis C virus-negative patients, respectively. The most frequent copy number alterations involved chromosomes 7 and 17 (21% and 24%, respectively). Except for Xp gain (P = .01), no differences in common alterations were found between hepatitis C virus-positive and hepatitis C virus-negative cases. Unmutated status of the IgV(H) gene was related to del(7q) (P = .04) and dup(12q) (P = .03). The high-risk group identified according to the new splenic marginal zone B-cell lymphoma prognostic score was associated with del(7q) (P = .01) and del(17p) (P = .02). Hepatitis C virus-positive splenic marginal zone B-cell lymphoma patients have no specific chromosome alterations. Patients with poor prognosis are characterized by distinctive imbalances.
Novara
Francesca
F
Department of Human end Hereditary Pathology, Section of Medical Genetics, University of Pavia, Pavia 27100, Italy.
Arcaini
Luca
L
Merli
Michele
M
Passamonti
Francesco
F
Zibellini
Silvia
S
Rizzi
Silvia
S
Rattotti
Sara
S
Rumi
Elisa
E
Pascutto
Cristiana
C
Vetro
Annalisa
A
Astori
Cesare
C
Boveri
Emanuela
E
Lucioni
Marco
M
Paulli
Marco
M
Zuffardi
Orsetta
O
Lazzarino
Mario
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055028:Comparative Genomic Hybridization; D004252:DNA Mutational Analysis; D005260:Female; D018628:Gene Dosage; D055106:Genome-Wide Association Study; D006526:Hepatitis C / Q000150:complications*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009154:Mutation
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region