ACL/MCL transection affects knee ligament insertion distance of healing and intact ligaments during gait in the Ovine model.
The objective of this study was to assess the impact of combined transection of the anterior cruciate and medial collateral ligaments on the intact and healing ligaments in the ovine stifle joint. In vivo 3D stifle joint kinematics were measured in eight sheep during treadmill walking (accuracy: 0.4+/-0.4mm, 0.4+/-0.4 degrees ). Kinematics were measured with the joint intact and at 2, 4, 8, 12, 16 and 20 weeks after either surgical ligament transection (n=5) or sham surgery without transection (n=3). After sacrifice at 20 weeks, the 3D subject-specific bone and ligament geometry were digitized, and the 3D distances between insertions (DBI) of ligaments during the dynamic in vivo motion were calculated. Anterior cruciate ligament/medial collateral ligament (ACL/MCL) transection resulted in changes in the DBI of not only the transected ACL, but also the intact lateral collateral ligament (LCL) and posterior cruciate ligament (PCL), while the DBI of the transected MCL was not significantly changed. Increases in the maximal ACL DBI (2 week: +4.2mm, 20 week: +5.7mm) caused increases in the range of ACL DBI (2 week: 3.6mm, 20 week: +3.8mm) and the ACL apparent strain (2 week: +18.9%, 20 week: +24.0%). Decreases in the minimal PCL DBI (2 week: -3.2mm, 20 week: -4.3mm) resulted in increases in the range of PCL DBI (2 week: +2.7mm, 20 week: +3.2mm). Decreases in the maximal LCL DBI (2 week: -1.0mm, 20 week: -2.0mm) caused decreased LCL apparent strain (2 week: -3.4%, 20 week: -6.9%). Changes in the mechanical environment of these ligaments may play a significant role in the biological changes observed in these ligaments.
Tapper
Janet E
JE
Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada. jtapper@ucalgary.ca
Funakoshi
Yusei
Y
Hariu
Mitsuhiro
M
Marchuk
Linda
L
Thornton
Gail M
GM
Ronsky
Janet L
JL
Zernicke
Ron
R
Shrive
Nigel G
NG
Frank
Cyril B
CB
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery*; D001696:Biomechanical Phenomena; D005684:Gait / Q000502:physiology*; D007718:Knee Injuries / Q000601:surgery; D007719:Knee Joint / Q000601:surgery; D008023:Ligaments, Articular; D017888:Medial Collateral Ligament, Knee / Q000601:surgery*; D012756:Sheep; D016138:Walking; D014945:Wound Healing