[Isolation and immunology identification of spermagglutinating antibodies from human serum].
OBJECTIVE
Isolation of spermagglutinating antibodies and their assesment.


METHODS
Retrospective study.


METHODS
Special consulting for reptoduction immunology, Department of Obstetrics and Gynecology, Charles University and Faculty Hospital, Plzen.


METHODS
Fractionation of serum samples by liquid exclusion chromatography, examination of full sera and their chromatographic fractions by Friberg teste (Tray Agglutination Test--TAT), indirect antiimmunoglobulin reaction test (i-MAR test) and by supplementar radial immunodiffusiona (RID).


RESULTS
Isolation of spermagglutinating fractions of antisperm antibodies positive sera preserved spermagglutinating aktivity and confirmed great spermagglutinating potential of IgM.


CONCLUSIONS
According to assesment of the presence of IgG and IgG we reported possible states of immunisation: actual immunisation with IgM activity, perpetual stimulation (IgG and IgM) and, finaly, anamnestic titres in IgG. These findings can help us to choose an optimal way of treatment. Excluding gel chromatography is suitable method for serum proteins fractionation, but not their identification--presence of antisperm antibodies does not affect the chromatographic spectrum, nor the RID patterns.
Cibulka
J
J
Gynekologicko-porodnická klinika LF UK a FN Plzen. cibulkaj@fnplzen.cz
Ulcova-Gallová
Z
Z
Balvín
M
M
Bibková
K
K
Micanová
Z
Z
D000372:Agglutination Tests; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D007246:Infertility / Q000276:immunology*; D008297:Male; D013073:Sperm Agglutination / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
D001323:Autoantibodies