Alkalibacillus flavidus sp. nov., isolated from a marine solar saltern.
A Gram-stain-positive, motile, rod-shaped bacterial strain, ISL-17(T), was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-17(T) grew optimally at pH 8.5-9.0, at 37 degrees C and in the presence of approximately 10 % (w/v) NaCl. It contained meso-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan, MK-7 as the predominant menaquinone and iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(16 : 0) as the major fatty acids. The DNA G+C content was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-17(T) fell within the genus Alkalibacillus, clustering with Alkalibacillus salilacus BH163(T) with a bootstrap resampling value of 100 %. Strain ISL-17(T) exhibited 98.2 % 16S rRNA gene sequence similarity to A. salilacus BH163(T) and 95.8-96.5 % similarity to the type strains of the other Alkalibacillus species. The mean DNA-DNA relatedness value between strain ISL-17(T) and A. salilacus KCTC 3916(T) was 19 %. The phenotypic properties of strain ISL-17(T), together with its phylogenetic and genetic distinctiveness, enable this strain to be differentiated from recognized Alkalibacillus species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-17(T) represents a novel species within the genus Alkalibacillus, for which the name Alkalibacillus flavidus sp. nov. is proposed; the type strain is ISL-17(T) (=KCTC 13258(T) =CCUG 56753(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Kang
So-Jung
SJ
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Jung
Yong-Taek
YT
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Lee
Mi-Hwa
MH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
D001406:Bacillaceae / Q000145:classification* / Q000235:genetics; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry; D007723:Korea; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000737:chemistry; D012623:Seawater / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride; D014871:Water Microbiology
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts; D012965:Sodium Chloride