Identification and characterization of human LYPD6, a new member of the Ly-6 superfamily.
The Ly-6 protein superfamily is usually identified as a group of proteins with a LU protein domain. LU domain is about 80 amino acids long and characterized by a conserved pattern of 10 cysteine residues. Here we report the cloning and characterization of a novel human LU domain containing gene, LYPD6, isolated from human testis cDNA library, and mapped to 2q23.1-23.2 by searching the UCSC genomic database. The LYPD6 cDNA sequence of 3,501 base pairs contains an open reading frame encoding 171 amino acids. Subcellular localization of LYPD6 demonstrated that the protein was localized in the cytoplasm when overexpressed in COS-7 cells. RT-PCR analysis showed that LYPD6 was widely expressed in human tissues and the expression levels in brain and heart were relatively high. Furthermore, the subsequent analysis based on reporter gene assays suggested that overexpression of LYPD6 in HEK 293T cells was able to suppress the transcriptional activities of AP1.
Zhang
Yifeng
Y
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Handan Road 220, 200433, Shanghai, People's Republic of China.
Lang
Qingyu
Q
Li
Jie
J
Xie
Fang
F
Wan
Bo
B
Yu
Long
L
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D002522:Chlorocebus aethiops; D002877:Chromosomes, Human / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005091:Exons / Q000235:genetics; D058851:GPI-Linked Proteins; D020869:Gene Expression Profiling; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013347:Subcellular Fractions / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D000950:Antigens, Ly; D058851:GPI-Linked Proteins; C549815:LYPD6 protein, human; D018808:Transcription Factor AP-1