A serum amyloid P-binding hydrogel speeds healing of partial thickness wounds in pigs.
During wound healing, some circulating monocytes enter the wound, differentiate into fibroblast-like cells called fibrocytes, and appear to then further differentiate into myofibroblasts, cells that play a key role in collagen deposition, cytokine release, and wound contraction. The differentiation of monocytes into fibrocytes is inhibited by the serum protein serum amyloid P (SAP). Depleting SAP at a wound site thus might speed wound healing. SAP binds to some types of agarose in the presence of Ca(2+). We found that human SAP binds to an agarose with a K(D) of 7 x 10(-8) M and a B(max) of 2.1 microg SAP/mg wet weight agarose. Mixing this agarose 1 : 5 w/v with 30 microg/mL human SAP (the average SAP concentration in normal serum) in a buffer containing 2 mM Ca(2+) reduced the free SAP concentration to approximately 0.02 microg/mL, well below the concentration that inhibits fibrocyte differentiation. Compared with a hydrogel dressing and a foam dressing, dressings containing this agarose and Ca(2+) significantly increased the speed of wound healing in partial thickness wounds in pigs. This suggests that agarose/Ca(2+) dressings may be beneficial for wound healing in humans.
Gomer
Richard H
RH
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005-1892, USA. richard@rice.edu
Pilling
Darrell
D
Kauvar
Lawrence M
LM
Ellsworth
Stote
S
Ronkainen
Sanna D
SD
Roife
David
D
Davis
Stephen C
SC
D000287:Administration, Topical; D000818:Animals; D001706:Biopsy; D002352:Carrier Proteins; D024042:Collagen Type I / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage*; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D000683:Serum Amyloid P-Component / Q000008:administration & dosage*; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013552:Swine; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D002352:Carrier Proteins; D024042:Collagen Type I; D000683:Serum Amyloid P-Component; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007633:Keratins