title: In vitro cytotoxicity of cyanobacteria from water ecosystems of Serbia.
abstract: OBJECTIVE
  The purpose of this study was to investigate whether water samples from water ecosystems of Serbia, unknown so far with regard to cyanotoxin levels, are the source of toxic compounds originating from the biological activity of cyanobacteria.
  
  
  METHODS
  The growth inhibition activity was evaluated using in vitro toxicity assay in Neuro-2a (mouse neuroblastoma) and MRC-5 (human fetal lung) cell lines, after 48 h of exposure time. Cell growth was evaluated by the colorimetric sulforhodamine B (SRB) assay.
  
  
  RESULTS
  Our experiments revealed that some of the investigated water samples are toxigenic and alter cell growth of Neuro-2 and MRC-5 cell lines in vitro.
  
  
  CONCLUSIONS
  Neuro-2a and MRC-5 cell lines responded to the presence of secondary metabolites of cyanobacteria. Significant cytotoxic effects were detected in the samples from lakes (Ludos and Palic), reservoirs (Zobnatica) and rivers (Krivaja).
authors: Oncology Institute of Vojvodina, Sremska Kamenica, Serbia. ddaaggeerr@gmail.com
authors: D
authors: D
authors: Cetojevic-Simin
authors: Z
authors: Z
authors: Svircev
authors: V V
authors: VV
authors: Baltic
mesh_terms: D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D000458:Cyanobacteria / Q000502:physiology*; D017753:Ecosystem; D005333:Fetus / Q000166:cytology / Q000382:microbiology*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000382:microbiology*; D051379:Mice; D009447:Neuroblastoma / Q000382:microbiology / Q000473:pathology*; D012235:Rhodamines / Q000032:analysis; D055771:Serbia; D014867:Water / Q000737:chemistry*
chemical_list: D012235:Rhodamines; D014867:Water; C022027:lissamine rhodamine B