Xenophilus aerolatus sp. nov., isolated from air.
A novel aerobic, Gram-negative, motile, rod-shaped bacterial strain designated 5516S-2(T) was isolated from an air sample taken in Suwon, Republic of Korea. Colonies were yellow-pigmented and circular with entire margins. 16S rRNA gene sequence analysis showed that strain 5516S-2(T) was closely related to Xylophilus ampelinus DSM 7250(T) (97.6 % sequence similarity), Variovorax soli KACC 11579(T) (97.5 %) and Xenophilus azovorans DSM 13620(T) (97.1 %). However, the phylogenetic tree indicated that strain 5516S-2(T) formed a separate clade from Xenophilus azovorans. Strain 5516S-2(T) displayed 42, 31 and 30 % DNA-DNA relatedness to the type strains of Xenophilus azovorans, Xylophilus ampelinus and V. soli, respectively. The major fatty acids (>10 % of total fatty acids) were C(16 : 0) (33.3 %), C(17 : 0) cyclo (18.8 %), C(18 : 1)omega7c (17.5 %) and summed feature 3 (comprising C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 13.0 %). The DNA G+C content was 69 mol%. The major quinone was ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminophospholipids. Genotypic and phenotypic characteristics clearly distinguished strain 5516S-2(T) from closely related species and indicated that it represents a novel species within the genus Xenophilus, for which the name Xenophilus aerolatus sp. nov. is proposed. The type strain is 5516S-2(T) (=KACC 12602(T)=DSM 19424(T)).
Kim
Soo-Jin
SJ
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Rural Development Administration, Suwon 441-707, Republic of Korea.
Kim
Yi-Seul
YS
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Rural Development Administration, Suwon 441-707, Republic of Korea.
Weon
Hang-Yeon
HY
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Rural Development Administration, Suwon 441-707, Republic of Korea.
Anandham
Rangasamy
R
Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai, India.
Noh
Hyung-Jun
HJ
Mushroom Research Division, National Institute of Horticultural & Medicinal Crop, Rural Development Administration, Suwon 441-707, Republic of Korea.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Rural Development Administration, Suwon 441-707, Republic of Korea.
D000391:Air Microbiology; D001482:Base Composition; D001483:Base Sequence; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S