Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia.
A moderately halophilic, motile, Gram-negative, rod-shaped bacterium, strain LC6(T), was isolated from a water sample of lake Laguna Colorada in the Bolivian Andes. The major cellular fatty acids were C(18 : 1)omega7c, iso-C(16 : 1)omega7c 2-OH, C(16 : 0) and C(12 : 0) 3-OH. The respiratory ubiquinones found in strain LC6(T) were Q-9 (97 %) and Q-8 (3 %). Strain LC6(T) was aerobic, heterotrophic, and able to utilize various carbohydrates and other substrates as carbon source. The G+C content of the genomic DNA of strain LC6(T) was 52.5 mol%. The organism was able to grow at pH 6.0-11.0 (optimum, pH 7.0-8.0), at 4-45 degrees C (optimum, 30-35 degrees C) and in the presence of 0.5-20 % (w/v) NaCl (optimum, 1-3 %, w/v). Based on 16S rRNA gene sequence analysis, strain LC6(T) was most closely related to Halomonas hydrothermalis DSM 15725(T) and Halomonas venusta DSM 4743(T) (98.8 % similarity), followed by Halomonas aquamarina DSM 30161(T), Halomonas axialensis DSM 15723(T) and Halomonas meridiana DSM 5425(T) (98.4 %). However, levels of DNA-DNA relatedness between strain LC6(T) and the above type strains were low (<31 %). Strain LC6(T) resembled recognized Halomonas species with respect to various physiological, biochemical and nutritional characteristics. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that strain LC6(T) represents a novel species of the genus Halomonas, for which the name Halomonas andesensis is proposed. The type strain is LC6(T) (=CCUG 54844(T)=LMG 24243(T)=DSM 19434(T)).
Guzmán
Daniel
D
Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia.|Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Quillaguamán
Jorge
J
Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia.|Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Muñoz
Marlene
M
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Hatti-Kaul
Rajni
R
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D001838:Bolivia; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D020459:Genes, rRNA; D020632:Halomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride