Inhibition of lipid peroxidation, cyclooxygenase enzyme and human tumor cell proliferation by compounds in herbal water.
A powdered mixture of dried herbs, "Panamrutham", is sold in India for the preparation of "herbal drinking water". The hot water extract of this herbal mixture gave lipid peroxidation (LPO), cyclo-oxygenase (COX-1 and -2) enzyme and human tumor cell proliferation inhibitory activities between 25 and 250 microg/mL. The bioassay-guided purification of the water extract afforded a novel compound (1), along with phenolics (2, 4, 6, and 7) and sesquiterpenoids (3 and 5). The isolates were evaluated for LPO, COX-1 and -2 enzyme and human tumor cell proliferation inhibitory activities. At 25 microg/mL, compounds 1-7 inhibited LPO by 22-73% and COX-1 and -2 enzymes by 3-14% and 14-74%, respectively. Compounds 5 and 6 at 25 microg/mL showed growth inhibition of colon, gastric, lung, breast and central nervous system human tumor cell lines by 60 and 67, 43 and 60, 24 and 64, 34 and 65, 6 and 27%, respectively. Compounds 2, 4 and 7 displayed weak or moderate growth inhibition of colon, gastric and breast human tumor cell lines. This is the first report on the LPO inhibitory activities of compounds 1 and 3-7 and the COX and tumor cell proliferation inhibitory activities of compounds 1, 3-5 and 7.
Liu
Yunbao
Y
Bioactive Natural Products and Phytoceuticals, Department of Horticulture and Toxicology Center, Michigan State University, East Lansing, Michigan, USA.
Mulabagal
Vanisree
V
Bowen-Forbes
Camille S
CS
Aviayan
Rejanish
R
Nair
Muraleedharan G
MG
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water
D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D016861:Cyclooxygenase Inhibitors; D010936:Plant Extracts; D014867:Water