Techniques used to study the DNA polymerase reaction pathway.
A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.
Joyce
Catherine M
CM
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, P.O. Box 208114, New Haven, CT 06520-8114, USA. catherine.joyce@yale.edu
D000818:Animals; D001681:Biological Assay; D004247:DNA / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013379:Substrate Specificity
D004247:DNA; D004259:DNA-Directed DNA Polymerase