Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis.
Site-directed incorporation of PEGylated nonnatural amino acids with 4, 8, and 12 repeated ethylene glycol units was examined in a cell-free translation system. PEGylated aminophenylalanine derivatives were successfully incorporated into proteins, whereas PEGylated lysines were not. The incorporation efficiency of the PEGylated amino acids decreased with an increase in PEG chain length. The present method will be useful for preparation of proteins which are PEGylated in a site-specific and quantitative manner.
Shozen
Naoki
N
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa, Japan.
Iijima
Issei
I
Hohsaka
Takahiro
T
D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics; D000926:Anticodon / Q000235:genetics; D002474:Cell-Free System; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D046188:Protein Modification, Translational; D011506:Proteins / Q000737:chemistry*; D012343:RNA, Transfer / Q000378:metabolism
D000596:Amino Acids; D000926:Anticodon; D011506:Proteins; D011092:Polyethylene Glycols; D012343:RNA, Transfer