[Expression of human beta-defensin-3 induced by lipopolysaccharide in human bronchial epithelial cells].
OBJECTIVE
To study the expression of human beta-defensin-3 (hBD-3) induced by lipopolysaccharide (LPS) in human bronchial epithelial (HBE) cells, and explore the role of hBD-3 in respiratory infection.


METHODS
HBE cells were stimulated with different concentrations of LPS (0.01, 0.1, 1 and 10 microg/mL). hBD-3 mRNA expression was detected by RT-PCR 2 hrs later. hBD-3 protein expression was detected by Western blot 4 hrs later.


RESULTS
hBD-3 mRNA and protein was weakly expressed in normal HBE cells. LPS stimulation resulted in a significant increase of hBD-3 mRNA and protein expression (p<0.01). hBD-3 mRNA and protein expression increased with increasing LPS concentrations. There were significant differences in the hBD-3 mRNA and protein expression in cells stimulated by different concentrations of LPS (p<0.05).


CONCLUSIONS
LPS can induce hBD-3 expression in a dose-dependent manner. hBD-3 might play a role in initial defensive reaction against bacterial invasion.
Li
Jia
J
Department of Pediatrics, Hunan Provincial People's Hospital, Changsha 410005, China.
Zhang
Bing
B
Zhong
Li-Li
LL
D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D023083:beta-Defensins / Q000032:analysis / Q000235:genetics*
C422950:DEFB103A protein, human; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D023083:beta-Defensins