A two-dimensional electrophoretic profile of the proteins secreted by Herbaspirillum seropedicae strain Z78.
Herbaspirillum seropedicae is an endophytic bacterium that associates with rice, sugarcane and other economically important crops. Secreted proteins play a key role in the plant-bacterial interaction. Using 2D electrophoresis and peptide mass fingerprint mass spectrometry, 63 protein spots representing 41 different secreted proteins were identified during growth of H. seropedicae under nitrogen-sufficient conditions. In silico analysis showed that 25.4% of the proteins had signal peptides and 15.9% were predicted to be non-classically secreted. Among the most abundant were flagellar components and ABC-type transport system proteins. Nine secreted proteins had also been identified in the cellular proteome, suggesting that they also play a role in the extracellular environment. No type III secreted proteins were detected by comparison of the wild type strain with an hrcN mutant strain.
Chaves
Daniela Fojo Seixas
DF
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, C. Postal 19046, 81531-990, Curitiba, PR, Brazil.
de Souza
Emanuel Maltempi
EM
Monteiro
Rose Adele
RA
de Oliveira Pedrosa
Fábio
F
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D042803:Herbaspirillum / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D013045:Species Specificity
D001426:Bacterial Proteins; D020543:Proteome