title: Increased production of ADAMTS13 in hepatic stellate cells contributes to enhanced plasma ADAMTS13 activity in rat models of cholestasis and steatohepatitis.
abstract: Although hepatic stellate cells, endothelial cells, glomerular podocytes and plateles were reported to be a source of ADAMTS13, it is not clarified which source is involved in the regulation of plasma ADAMTS13 activity. It was demonstrated previously that selective hepatic stellate cell damage in rats caused decreased plasma ADAMTS13 activity. To further elucidate the potential contribution of hepatic stellate cells to the regulation of plasma ADAMTS13 activity, this study examined plasma ADAMTS13 activity when hepatic stellate cells proliferate during the process of liver fibrosis by employing rat models of liver fibrosis due to cholestasis, bile duct ligation, and steatohepatitis, a choline-deficient L-amino acid-defined-diet. ADAMTS13 expression was increased with co-localisation with smooth muscle alpha-actin, a marker of hepatic stellate cells, in bile duct-ligated livers up to four weeks, in which a close correlation between ADAMTS13 and smooth muscle alpha-actin mRNA expressions was determined. Plasma ADAMTS13 activity, measured by a sandwich ELISA involving a specific substrate to ADAMTS13, was increased in bile duct-ligated rats with a significant correlation with ADAMTS13 mRNA expression levels in the liver. Furthermore, ADAMTS13 mRNA expression was increased with enhanced mRNA expression in smooth muscle alpha-actin in the livers of rats fed a choline-deficient L-amino acid-defined-diet for 16 weeks, in which increased plasma ADAMTS13 activity was determined. Thus, increased plasma ADAMTS13 activity in cholestasis and steatohepatitis in rats may be due, at least in part, to enhanced ADAMTS13 production in the liver, suggesting a significant role of hepatic stellate cells in the regulation of plasma ADAMTS13 activity.
authors: Department of Clinical Laboratory Medicine, The University of Tokyo, Tokyo 113-8655, Japan.
authors: Naoko
authors: N
authors: Watanabe
authors: Hitoshi
authors: H
authors: Ikeda
authors: Yukio
authors: Y
authors: Kume
authors: Yumiko
authors: Y
authors: Satoh
authors: Makoto
authors: M
authors: Kaneko
authors: Daiya
authors: D
authors: Takai
authors: Kazuaki
authors: K
authors: Tejima
authors: Masakazu
authors: M
authors: Nagamine
authors: Hirosato
authors: H
authors: Mashima
authors: Tomoaki
authors: T
authors: Tomiya
authors: Eisei
authors: E
authors: Noiri
authors: Masao
authors: M
authors: Omata
authors: Masanori
authors: M
authors: Matsumoto
authors: Yoshihiro
authors: Y
authors: Fujimura
authors: Yutaka
authors: Y
authors: Yatomi
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics; D000071120:ADAMTS13 Protein; D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002779:Cholestasis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D017931:DNA Primers / Q000235:genetics; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D055166:Hepatic Stellate Cells / Q000201:enzymology* / Q000473:pathology; D006520:Hepatitis, Animal / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; D000199:Actins; D017931:DNA Primers; D012333:RNA, Messenger; C498862:Acta2 protein, rat; C512067:Adamts13 protein, rat