Nicotine can skew the characterization of the macrophage type-1 (MPhi1) phenotype differentiated with granulocyte-macrophage colony-stimulating factor to the MPhi2 phenotype.
Macrophages (MPhis) exhibit functional heterogeneity and plasticity in the local microenvironment. Recently, it was reported that MPhis can be divided into proinflammatory MPhis (MPhi1) and anti-inflammatory MPhis (MPhi2) based on their polarized functional properties. Here, we report that nicotine, the major ingredient of cigarette smoke, can modulate the characteristics of MPhi1. Granulocyte-macrophage colony-stimulating factor-driven MPhi1 with nicotine (Ni-MPhi1) showed the phenotypic characteristics of MPhi2. Like MPhi2, Ni-MPhi1 exhibited antigen-uptake activities. Ni-MPhi1 suppressed IL-12, but maintained IL-10 and produced high amounts of MCP-1 upon lipopolysaccharide stimulation compared with MPhi1. Moreover, we observed strong proliferative responses of T cells to lipopolysaccharide-stimulated MPhi1, whereas Ni-MPhi1 reduced T cell proliferation and inhibited IFN-gamma production by T cells. These results suggest that nicotine can change the functional characteristics of MPhi and skew the MPhi1 phenotype to MPhi2. We propose that nicotine is a potent regulator that modulates immune responses in microenvironments.
Yanagita
Manabu
M
Department of Periodontology, Osaka University Graduate School of Dentistry, Japan.
Kobayashi
Ryohei
R
Murakami
Shinya
S
D000941:Antigens / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D006801:Humans; D007109:Immunity / Q000187:drug effects*; D018664:Interleukin-12 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D009538:Nicotine / Q000494:pharmacology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D008070:Lipopolysaccharides; D018664:Interleukin-12; D009538:Nicotine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor