In vitro covalent binding proteins of zerumbone, a chemopreventive food factor.
Zerumbone (ZER), a sesquiterpene in Zingiber zerumbet Smith, has been implicated as a promising chemopreventive agent. Here we found that ZER suppressed expression of pro-inflammatory genes (COX-2 and iNOS) and induced detoxification genes (GSTP1 and NQO1) in RAW264.7 macrophages. Using the ZER-bound Sepharose gel, it appeared ZER was covalently bound to proteins, Keap1 and HuR, that play key roles in these molecular mechanisms.
Ohnishi
Kohta
K
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan.
Irie
Kazuhiro
K
Murakami
Akira
A
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D018890:Chemoprevention; D005502:Food; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D030521:Zingiberaceae / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D011506:Proteins; D012717:Sesquiterpenes; D013438:Sulfhydryl Compounds; C403304:zerumbone