Differential gene expression signatures of adult peripheral blood vs cord blood monocyte-derived immature and mature dendritic cells.
OBJECTIVE
Our previous studies have demonstrated differentially expressed genetic signature patterns in adult peripheral blood (APB) monocytes (Mos) vs cord blood (CB) Mos.


METHODS
In this study, we examined the differential gene expression profiles of APB vs CB immature dendritic cells (iDCs) and mature dendritic cells (mDCs) derived from Mos.


RESULTS
By utilizing oligonucleotide microarray, significant differential gene expression signature patterns were identified in APB vs CB mDCs, including increased expression of chemokines, cytokine receptors, and cell surface molecules. Additionally, signaling protein genes were significantly overexpressed in APB vs CB mDCs. There was also a significant amplification of expression of transcription factor interferon (IFN) regulatory factors and structure regulatory genes in APB vs CB mDCs. In contrast, there were genes expressed significantly higher in CB vs APB mDCs, including cell-cycle regulators and signaling molecule gene.


CONCLUSIONS
Taken together, these results suggest that specific genetic signatures might be responsible for differential DC differentiation and maturation between APB vs CB, and may provide insight into molecular mechanisms regulating differential immune responses between neonates and adults.
Jiang
Hong
H
Department of Pediatrics, Morgan Stanley Children's Hospital New York Presbyterian, Columbia University, New York, NY 10032, USA.
van de Ven
Carmella
C
Baxi
Laxmi
L
Satwani
Prakash
P
Cairo
Mitchell S
MS
D000328:Adult; D017951:Antigen Presentation; D001773:Blood Cells / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D009000:Monocytes / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism
D018797:Cell Cycle Proteins; D018925:Chemokines; D047908:Intracellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D018121:Receptors, Cytokine; D007371:Interferon-gamma