title: Development of a stir bar sorptive extraction based HPLC-FLD method for the quantification of serotonin reuptake inhibitors in plasma, urine and brain tissue samples.
abstract: The aim of this article is to present an analytical application of stir bar sorptive extraction (SBSE) coupled to HPLC-fluorescence detection (FLD) for the quantification of fluoxetine (FLX), citalopram (CIT) and venlafaxine (VLF) and their active metabolites - norfluoxetine (NFLX), desmethyl- (DCIT) and didesmethylcitalopram (DDCIT) and o-desmethylvenlafaxine (ODV) - in plasma, urine and brain tissue samples. All the parameters influencing adsorption (pH, ion strength, organic modifier addition, volume, extraction time and temperature) and desorption (desorption solvent composition, time, temperature and desorption mode) of the analytes on the stir bar have been optimized. For each matrix, the analytical method has been assessed by studying the linearity and the intra- and interday accuracy (89-113%) and precision (RSD<13%). The improvement of the quantification limits (0.2-2 microg l(-1) for plasma, 2-20 ng g(-1) for brain tissue and 1-10 microg l(-1) for urine, depending on the respective response for analytes) and the development of a procedure for all the matrices make this method useful in clinical and forensic analysis.
authors: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, Vitoria-Gasteiz (Alava), Spain.
authors: Nora
authors: N
authors: Unceta
authors: Ana
authors: A
authors: Ugarte
authors: Alicia
authors: A
authors: Sánchez
authors: Alberto
authors: A
authors: Gómez-Caballero
authors: Maria Aránzazu
authors: MA
authors: Goicolea
authors: Ramón J
authors: RJ
authors: Barrio
mesh_terms: D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000032:analysis* / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015283:Citalopram / Q000032:analysis / Q000378:metabolism; D003511:Cyclohexanols / Q000378:metabolism; D005453:Fluorescence; D005473:Fluoxetine / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis* / Q000378:metabolism; D000069470:Venlafaxine Hydrochloride
chemical_list: D018687:Antidepressive Agents, Second-Generation; D015283:Citalopram; D003511:Cyclohexanols; D005473:Fluoxetine; D017367:Selective Serotonin Reuptake Inhibitors; D000069470:Venlafaxine Hydrochloride