Gram-negative bacteria associated with brewery yeasts: reclassification of Obesumbacterium proteus biogroup 2 as Shimwellia pseudoproteus gen. nov., sp. nov., and transfer of Escherichia blattae to Shimwellia blattae comb. nov.
Phylogenetic analyses of type and reference strains of Obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16S rRNA gene sequences and partial sequences of four protein-coding genes (fusA, leuS, pyrG and rpoB). Both approaches revealed that O. proteus biogroup 1 strains were closely related to Hafnia alvei. Biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included Escherichia blattae. Phenotypic tests were consistent with the molecular classification and provided diagnostic features. It is proposed that biogroup 2 strains be placed in a new genus, Shimwellia gen. nov., as Shimwellia pseudoproteus sp. nov., with strain 521(T) (=DSM 3038(T)=LMG 24835(T)=NCIMB 14534(T)) as the type strain, and that Escherichia blattae be transferred to the genus Shimwellia as Shimwellia blattae comb. nov., with strain ATCC 29907( T) (=DSM 4481(T)) as the type strain.
Priest
Fergus G
FG
International Centre for Brewing and Distilling, Heriot Watt University, Edinburgh EH14 4AS, UK.
Barker
Margaret
M
International Centre for Brewing and Distilling, Heriot Watt University, Edinburgh EH14 4AS, UK.
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001515:Beer / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004925:Escherichia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020459:Genes, rRNA; D005838:Genotype; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S