[Influce of lysophosphatidic acid on proliferation, adhesion, migration and apoptosis of cervical cancer HeLa cells].
OBJECTIVE
To observe the effect of lysophosphatidic acid on cervical cancer cell line HeLa cells proliferation, adhesion, migration and apoptosis and study related signaling pathway.


METHODS
After HeLa cells affected by LPA, the level of proliferation was drawing growth curve after cell couting; the sensitivity to cisplatin was evaluated by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry was used to examine cell apoptosis; the potentiality of migration was evaluated by transwell chamber, then to observe the effect of Y27632, LY294002 and PD98059 on biological function of LPA.


RESULTS
LPA could promote HeLa cell proliferate on time-dependent and dose-dependent relationship. MEK1 inhibitor could inhibit LPA-induced cell proliferation; LPA promoted the adhesion and migration of HeLa cells in dose-dependence; Rho inhibitor could inhibit the LPA-induced adhesion and migration; LPA could reduce the chemotherapy sensibility of cervical cancer to DDP in dose-dependence, then PI3K inhibitor could resist this effect.


CONCLUSIONS
LPA could promote HeLa cells' proliferation, adhesion, migration and anti-apoptosis through multiple signaling pathways.
Sun
Hong
H
Department of Oncology, the First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
Zhu
Qing
Q
Ren
Juan
J
Wu
Lei
L
Kong
Fang-zhong
FZ
Li
Guang
G
Hu
Hai-feng
HF
D017209:Apoptosis; D002448:Cell Adhesion; D049109:Cell Proliferation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008246:Lysophospholipids / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000503:physiopathology*
D008246:Lysophospholipids; C032881:lysophosphatidic acid