title: Proteomic analysis of the interactions between Mycoplasma hyopneumoniae and porcine tracheal ciliated cells.
abstract: Mycoplasma hyopneumoniae colonizes at the porcine respiratory-ciliated epithelial cells and causes the enzootic pneumonia of swine. The adhesion step is crucial in the colonization process. A few adhesion molecules have been characterized, and the concurrent receptors from the porcine ciliated cells have also been suggested to recognize the adhesion molecules. In the present study, the interactions between M. hyopneumoniae and porcine tracheal ciliated cells were investigated by employing the Far-Western blotting method. The results indicate that aconitase, lamin A/C, and peroxiredoxin of the porcine tracheal ciliated cell may interact specifically with the mycoplasmal proteins. We speculate that these mycoplasmal proteins could be secreted cleavage products, and their relative small size may enable them to penetrate into ciliated cells interfering with important metabolic pathways and other critical cellular processes.
authors: Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien, 974, Taiwan, Republic of China.
authors: Yuan-Zuo
authors: YZ
authors: Li
authors: Yen-Peng
authors: YP
authors: Ho
authors: Shui-Tein
authors: ST
authors: Chen
authors: David
authors: D
authors: Shiuan
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D026481:Blotting, Far-Western; D002478:Cells, Cultured; D002923:Cilia / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D054884:Host-Pathogen Interactions; D006801:Humans; D009175:Mycoplasma Infections / Q000378:metabolism; D045705:Mycoplasma hyopneumoniae / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa; D014132:Trachea / Q000166:cytology*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome