Neural stem cells express Oct-3/4.
There remain controversy and disagreement on whether Oct-3/4 is expressed in neural stem cells or not. Although many reports had shown expression of Oct-3/4 in somatic stem and progenitor cells, conditional KO mice of Oct-3/4 in neural stem cells did not show any phenotype. Even pseudogenes are suspected for the "false positive" results. However, we here report that Oct-3/4 but not pseudogenes is actually expressed in neural stem cells. Western blot analysis with multiple Oct-3/4 antibodies also showed protein expression of Oct-3/4. The subnuclear localization of Oct-3/4 is clearly different from that of P19 cells, suggesting that Oct-3/4 might be inactivated by other mechanisms than transcriptional repression.
Chin
Ji-Hyang
JH
Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Shiwaku
Hiroki
H
Goda
Olga
O
Komuro
Akihiko
A
Okazawa
Hitoshi
H
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis* / Q000235:genetics; D011544:Pseudogenes; D013234:Stem Cells / Q000378:metabolism*; D014158:Transcription, Genetic
D050814:Octamer Transcription Factor-3