title: Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor.
abstract: BACKGROUND
  In response to pathogen attack, grapevine synthesizes phytoalexins belonging to the family of stilbenes. Grapevine cell cultures represent a good model system for studying the basic mechanisms of plant response to biotic and abiotic elicitors. Among these, modified beta-cyclodextrins seem to act as true elicitors inducing strong production of the stilbene resveratrol.
  
  
  RESULTS
  The transcriptome changes of Vitis riparia x Vitis berlandieri grapevine cells in response to the modified beta-cyclodextrin, DIMEB, were analyzed 2 and 6 h after treatment using a suppression subtractive hybridization experiment and a microarray analysis respectively. At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases. At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.
  
  
  CONCLUSIONS
  We report the first large-scale study of the molecular effects of DIMEB, a resveratrol inducer, on grapevine cell cultures. This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.
authors: IASMA Research and Innovation Center, Fondazione Edmund Mach, S. Michele a/Adige I-38010, Italy. anita.zamboni@univr.it
authors: Anita
authors: A
authors: Zamboni
authors: Pamela
authors: P
authors: Gatto
authors: Alessandro
authors: A
authors: Cestaro
authors: Stefania
authors: S
authors: Pilati
authors: Roberto
authors: R
authors: Viola
authors: Fulvio
authors: F
authors: Mattivi
authors: Claudio
authors: C
authors: Moser
authors: Riccardo
authors: R
authors: Velasco
mesh_terms: D002455:Cell Division; D002473:Cell Wall / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D018749:RNA, Plant / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000494:pharmacology*
chemical_list: D018076:DNA, Complementary; D018749:RNA, Plant; D013267:Stilbenes; D047392:beta-Cyclodextrins; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin; D000077185:Resveratrol