Fluorinated lipid constructs permit facile passage of molecular cargo into living cells.
Fluorinated lipids get rapidly internalized into living cells and are also displayed on the cell surface. The uptake of lipids is energy dependent and is likely via the clathrin-mediated endocytic pathway. Fluorinated lipids are 3-5-fold more efficient in acting as molecular transporters of noncovalently bound proteins than their hydrocarbon counterparts. These materials could serve as efficient molecular transporters for molecules that function in the cytoplasm such as short interfering RNAs (siRNAs).
Dafik
Laila
L
Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Kalsani
Venkateshwarlu
V
Leung
Anthony Kar Lun
AK
Kumar
Krishna
K
D001692:Biological Transport; D002470:Cell Survival; D004705:Endocytosis; D005434:Flow Cytometry; D054879:Halogenation; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D010714:Phosphatidylethanolamines / Q000737:chemistry* / Q000378:metabolism*
D010714:Phosphatidylethanolamines; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine