Can rhBMP-2 containing collagen sponges enhance bone repair in ovariectomized rats?: a preliminary study.
With an aging population the frequency of postmenopausal fractures is increasing. Methods to enhance the repair of osteoporotic bone repair therefore become more important to reduce the society burden of care. We asked if absorbable collagen sponges containing recombinant human bone morphogenetic protein-2 (rhBMP-2) have the potential to enhance bone repair. We randomly assigned 40 rats into the ovariectomy and sham operation groups. A segmental defect was created in the right tibia 12 weeks after ovariectomy. rhBMP-2-containing absorbable collagen sponges were implanted into the defect in half of the animals in each group. We analyzed radiographs and histological sections and performed three-point bending tests to assess repair. Radiological scores in the rhBMP-2 applied rats were higher than those in controls at the end of 8 weeks after tibial osteotomy. The specimens failed under higher loads in the rhBMP-2-applied groups and histology revealed a higher fracture healing score, including callus formation, bone union, marrow changes, and cortex remodeling. We observed no adverse tissue responses such as fibrous connective tissue formation and inflammatory cellular infiltration. rhBMP-2 in absorbable collagen sponges enhanced bone repair in segmental tibial defects of ovariectomized rats. The sponges with rhBMP-2 appeared to enhance bone repair.
Sarban
Sezgin
S
Department of Orthopaedic Surgery, Harran University Faculty of Medicine, Sanliurfa, Turkey.
Senkoylu
Alparslan
A
Isikan
U Erdem
UE
Korkusuz
Petek
P
Korkusuz
Feza
F
D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D016723:Bone Remodeling / Q000187:drug effects; D002146:Bony Callus / Q000187:drug effects / Q000503:physiopathology; D003094:Collagen; D004195:Disease Models, Animal; D004337:Drug Carriers; D005260:Female; D017102:Fracture Healing / Q000187:drug effects*; D006801:Humans; D010052:Ovariectomy; D010865:Pilot Projects; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage; D015503:Surgical Sponges; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000503:physiopathology / Q000601:surgery; D013978:Tibial Fractures / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004337:Drug Carriers; D011994:Recombinant Proteins; D003094:Collagen