In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror.
We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice.
Piyawattanametha
Wibool
W
James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, Stanford, California 94305, USA.
Cocker
Eric D
ED
Burns
Laurie D
LD
Barretto
Robert P
RP
Jung
Juergen C
JC
Ra
Hyejun
H
Solgaard
Olav
O
Schnitzer
Mark J
MJ
D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000166:cytology*; D002196:Capillaries / Q000166:cytology*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007909:Lenses; D051379:Mice; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity