[Growth inhibition and mechanism of cetyltrimethyl ammonium chloride on Chlorella vulgaris].
Growth inhibition of cetyltrimethyl ammonium chloride (CTAC), a cationic surfactants, on Chlorella vulgaris was investigated at batch culture in laboratory. Furthermore, the corresponding mechanisms were studied by the determination of absorption capacity, Zeta potential, activity of acid phosphatase and ultrastructure of algae. Results show that the growth inhibition by CATC is enhanced with its concentration increasing from 0.1 mg/L to 1 mg/L, and 96 h-EC50 of CTAC is 0.18 mg/L. In the presence of 0.3 mg/L CTAC in 8 d, the inhibition efficiency of biomass reaches 70.7%. Meanwhile, the absorption of nitrogen and iron is inhibited 83.9% and 86.2% respectively with Zeta potential of algae cell increasing from -12.5 mV to -6.7 mV. Furthermore, the relative activity of acid phosphatase declines to 23.1% at the same time. Plasmolysis, distortion of pyrenoid and swelling of lysosome is observed in the cell. Above phenomena indicates that CTAC increases the Zeta potential of algae cell and thus inhibites the absorption of nitrogen and iron. In addition, CTAC may affect the metabolism of phosphorus and change the ultrastructure of algae cell.
Xu
Yin
Y
Department of Environmental Science and Engineering, Xiangtan University, Xiangtan 411105, China. xuyin123@hotmail.com
Ge
Fei
F
Tao
Neng-Guo
NG
Zhu
Run-Liang
RL
Wang
Na
N
D008725:Bis-Trimethylammonium Compounds / Q000494:pharmacology*; D048408:Chlorella vulgaris / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D046508:Culture Techniques; D004784:Environmental Monitoring / Q000379:methods*; D006131:Growth Inhibitors; D013501:Surface-Active Agents / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000494:pharmacology
D008725:Bis-Trimethylammonium Compounds; D006131:Growth Inhibitors; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C525618:cethyltrimethylammonium chloride