Effects of epitope sequence tandem repeat and proline incorporation on polyclonal antibody production against cytochrome 1A2 and 3A4.
We describe a method for producing polyclonal antibodies against peptide antigen cytochrome P450 1A2 and 3A4 using a tandem repeat of the epitope region and incorporation of proline residue between the repeated sequences. An ELISA assay revealed more efficient generation of polyclonal antibodies to tandem repeat peptide antigens than mono-epitope peptides. The incorporation of proline residues further stimulated antibody production.
Ahn
Taeho
T
Department of Biochemistry, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. thahn@chonnam.ac.kr
Yun
Chul-Ho
CH
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000502:physiology; D019388:Cytochrome P-450 CYP1A2 / Q000737:chemistry / Q000276:immunology*; D051544:Cytochrome P-450 CYP3A / Q000737:chemistry / Q000276:immunology*; D004526:Efficiency; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D011392:Proline / Q000276:immunology / Q000502:physiology*; D020449:Repetitive Sequences, Amino Acid / Q000276:immunology*; D020080:Tandem Repeat Sequences / Q000502:physiology
D000939:Epitopes; D010446:Peptide Fragments; D011392:Proline; D019388:Cytochrome P-450 CYP1A2; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human