CelluSpots: a reproducible means of making peptide arrays for the determination of SH2 domain binding specificity.
Peptide arrays differ from conventional peptide synthesis in that hundreds upon thousands of peptides are synthesized and presented on a planar surface at a time. While direct synthesis of peptide arrays on a functionalized surface is feasible, reprinting of pre-made peptides offers flexibility and reproducibility and drastically reduces cost when multiple copies of the same or related peptide arrays are needed. CelluSpot, a method developed by Intavis, opens a new route in peptide array synthesis and printing and overcomes certain limitations of the SPOT membrane. This technique was used to produce hundreds of phosphotyrosine-oriented peptide array libraries for determining the specificity of the Src homology 2 (SH2) domain.
Wu
Chenggang
C
Department of Biochemistry and the Siebens-Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.
Li
Shawn S-C
SS
D000818:Animals; D002482:Cellulose / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis / Q000493:pharmacokinetics; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D013379:Substrate Specificity; D018909:src Homology Domains
D008567:Membranes, Artificial; D010455:Peptides; D002482:Cellulose