Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.
Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.
Takimoto
Jeffrey K
JK
The Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA.
Adams
Katrina L
KL
Xiang
Zheng
Z
Wang
Lei
L
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001386:Azides / Q000378:metabolism; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000378:metabolism; D013045:Species Specificity; D014162:Transfection
D000596:Amino Acids; D001386:Azides; D003432:Cross-Linking Reagents; C027162:4-azidophenylalanine; D010649:Phenylalanine; D000604:Amino Acyl-tRNA Synthetases