[Study on the chemical constituents of Sarcandra glabra by HPLC-ESI-MS/MS].
OBJECTIVE
To study and compare the main chemical constituents of Sarcandra glabra and qingrexiaoyanning capsules which were extracted by acetic ether.


METHODS
The sample solution were analyzed by a Zorbax C18 column with a gradient mobile phase composed of acetonitrile and 0.1% formic acid solution. Both UV and mass spectrometry detector were used simutaneously, full-scan detection mode was evaluated for the identification of all LC peaks.


RESULTS
We analyzed the mass spectrum of every LC peak and identified 26 molecular mass from the ion chromatogram of Sarcandra glabra extraction and 16 molecular mass from the extractions of qingrexiaoyanning capsule. 5 compounds were identified.


CONCLUSIONS
High performance liquid chromatography-mass/mass spectrometry has special advantages on analyzing the chemical constituents of traditional Chinese medicine.
Luo
Qi-Zhi
QZ
Southern Medical University, Guangzhou 510515, China. luoqizhi@fimmun.com
Dai
Kai-Jin
KJ
Ma
An-De
AD
D002109:Caffeic Acids / Q000032:analysis / Q000737:chemistry; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis / Q000737:chemistry; D003374:Coumarins / Q000032:analysis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D019684:Magnoliopsida / Q000737:chemistry*; D008970:Molecular Weight; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D002109:Caffeic Acids; D002214:Capsules; D002934:Cinnamates; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D012997:Solvents; C029010:cinnamic acid; C040048:caffeic acid