title: Carbon nanotubes based electrochemical biosensor for detection of formaldehyde released from a cancer cell line treated with formaldehyde-releasing anticancer prodrugs.
abstract: This paper reports the development of an electrochemical biosensor for the detection of formaldehyde in aqueous solution, based on the coupling of the enzyme formaldehyde dehydrogenase and a carbon nanotubes (CNT)-modified screen-printed electrode (SPE). We monitored the amperometric response to formaldehyde released from U251 human glioblastoma cells situated in the biosensor chamber in response to treatment with various anticancer prodrugs of formaldehyde and butyric acid. The current response was higher for prodrugs that release two molecules of formaldehyde (AN-193) than for prodrugs that release only one molecule of formaldehyde (AN-1, AN-7). Homologous prodrugs that release one (AN-88) or two (AN-191) molecules of acetaldehyde, showed no signal. The sensor is rapid, sensitive, selective, inexpensive and disposable, as well as simple to manufacture and operate.
authors: Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Israel.
authors: Lilach
authors: L
authors: Bareket
authors: Ada
authors: A
authors: Rephaeli
authors: Gili
authors: G
authors: Berkovitch
authors: Abraham
authors: A
authors: Nudelman
authors: Judith
authors: J
authors: Rishpon
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020148:Butyric Acid / Q000378:metabolism*; D045744:Cell Line, Tumor; D004563:Electrochemistry; D004566:Electrodes; D005557:Formaldehyde / Q000378:metabolism*; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D009243:NAD / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011355:Prodrugs / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D037742:Nanotubes, Carbon; D011355:Prodrugs; D009243:NAD; D020148:Butyric Acid; D005557:Formaldehyde; D000079:Acetaldehyde