Dielectrophoretic technique for measurement of chemical and biological interactions.
We present a novel dielectrophoretic technique that can be used to characterize molecular interactions inside a microfluidic device. Our approach allows functionalized beads which are initially at rest on a functionalized surface to be pulled away from the surface by the dielectrophoretic force acting on the beads. As a result, the interaction between the molecules on the surface and the beads can be quantitatively examined. We report detailed experimental results and validate the results with a model to show that the technique can be used to measure forces of interaction between molecules under various experimental conditions.
Baek
Sang Hyun
SH
Department of Biomedical Engineering, Yonsei University, Wonju 220-710, Korea.
Chang
Woo-Jin
WJ
Baek
Ju-Yeoul
JY
Yoon
Dae Sung
DS
Bashir
Rashid
R
Lee
Sang Woo
SW
D004563:Electrochemistry / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D008239:Lysine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013387:Succinic Anhydrides / Q000737:chemistry; D013499:Surface Properties
D013387:Succinic Anhydrides; C031801:succinic anhydride; D012822:Silicon Dioxide; D008239:Lysine