Thymic stromal lymphopoietin is a critical mediator of IL-13-driven allergic inflammation.
Both thymic stromal lymphopoietin (TSLP) and IL-13 are essential cytokines for the development of allergic inflammation. However, a causal link between TSLP and IL-13 has not yet been fully elucidated. This study aimed to investigate whether IL-13 induces TSLP expression and whether the induction contributes to the development of allergic inflammation. We found that IL-13 induced TSLP expression in mouse nasal tissue specimens in a Stat6-dependent manner. In addition, intranasal challenge of mice with IL-13 induced TSLP expression in the nasal epithelium. Importantly, intranasal IL-13 challenge induced eosinophilia and goblet cell hyperplasia in the nasal mucosa in mice, which was inhibited by the blockade of TSLP activity with anti-TSLP Ab. These findings suggest that TSLP is an important mediator of IL-13-driven allergic inflammation in the nasal mucosa. Taken together with the recent findings that IL-13 is a critical downstream element for TSLP-driven allergic inflammation, TSLP may function both upstream and downstream of IL-13, thus providing an additional rationale as to why TSLP plays such a central role in the development of allergic inflammation.
Miyata
Masanori
M
Department of Immunology, University of Yamanashi Faculty of Medicine, Yamanashi, Japan.
Nakamura
Yuki
Y
Shimokawa
Naomi
N
Ohnuma
Yuko
Y
Katoh
Ryohei
R
Matsuoka
Shuji
S
Okumura
Ko
K
Ogawa
Hideoki
H
Masuyama
Keisuke
K
Nakao
Atsuhito
A
D000818:Animals; D016207:Cytokines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006967:Hypersensitivity / Q000276:immunology*; D007249:Inflammation / Q000276:immunology*; D018793:Interleukin-13 / Q000276:immunology; D051379:Mice; D009297:Nasal Mucosa / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000094632:Thymic Stromal Lymphopoietin
D016207:Cytokines; D018793:Interleukin-13; D000094632:Thymic Stromal Lymphopoietin; C000721967:TSLP protein, mouse