[Bioremediation of petroleum hydrocarbon contaminated soil by bioaugmentation products].
In an experimental investigation of bioaugmentation products affected on the petroleum contaminated soil. The influence of the bioaugmentation products dose, injections and temperature on bioremediation were studied. The results showed that the degradation rate was related positively to the amount of inoculation, when the dose was increased to 0.6 mg x kg(-1), total petroleum hydrocarbon (TPH) degradation rate was 87% in 48 days. The results of GC-MS indicated that the dominant petroleum constituents in oil-contaminated raw soil were 82.1% n-alkane, 16% alkene and little of others hydrocarbons, such as carotane, alkylnaphthalenes, hopanes, and steranes. The peaks amount of GC profile decreased from 32 to 14 after 40 days of bioremediation, this result indicated that branched alkanes, alkene, and alkylnaphthalenes were thoroughly degraded, then line alkanes, hopanes, and steranes were left in soil. In addition, the longer part of n-alkane were degraded with rate relatively higher, while the residual fraction at the end of the test is shorter part of n-alkane because bacteria degraded the longer n-alkane to shorter. The shorter n-alkane concentration decreased with increasing inoculation. One time injection of bioaugmentation products into soil clearly improved the biodegradation efficiency higher than injection of bioaugmentation products in turn. Soil temperature also affected TPH degradation rate when it was 30 degrees C, TPH rate reached 80%, where as when it was 20 degrees C, the TPH rate was lower to 60%, which indicated higher temperature improved TPH degradation and accelerated bioremediation.
Huang
Ting-Lin
TL
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. huangtinglin@xauat.edu.cn
Xu
Jin-Lan
JL
Tang
Zhi-Xin
ZX
Xiao
Zhou-Qiang
ZQ
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial / Q000379:methods; D052918:Environmental Restoration and Remediation / Q000379:methods*; D010578:Petroleum / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*
D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants