[Differential expression of immunohistochemical markers between cardiac carcinoma and carcinoma in antrum of stomach and correlation thereof with clinicopathological factors].
OBJECTIVE
To study the differences in expression of several common immunohistochemical markers: epidermal growth factor receptor (EGFR), topoisomerase II (TOPOII), glutathione S-transferase pi (GST-pi), proliferation cell nuclear antigen ( PCNA), Her-2/Neu, P27, P21, and P53, between cardiac carcinoma and carcinoma in antrum of stomach and the correlation thereof with clinicopathological factors.


METHODS
211 paraffin-embedded tissue samples of gastric cancer were randomly chosen from the patients of cardium and stomach operated in 2005, including 110 cases of cardiac carcinoma and 101 cases of carcinoma in antrum of stomach. Immunohistochemical Envision two step method was used to detect the expression of the above mentioned markers.


RESULTS
Compared to the cardiac carcinoma, the carcinoma in antrum of stomach showed higher rate of clinicopathological staging was significantly higher, degree of differentiation lower, invasion deeper, and degree of lymph node metastasis higher (all P < 0.05). The expression rates of P21, Her-2/Neu, and GST-pi of the cardiac carcinoma group were 80.0% (88/110), 30.9% (34/110), and 92.7% (76/82) respectively, all significantly higher than those of the carcinoma in antrum of stomach group [67.0% (65/97), 16.7% (16/96), and 74.5% (41/55) respectively, P = 0.034, 0.017, and 0.003 respectively]. In the cardiac carcinoma, P21 expression was positively correlated with the expression of P27 and EGFR, the P27 expression was positively correlated with the expression of EGFR and TOPOII, and the GST-pi expression was positively correlated with the TOPOII expression (r = 0.255, P = 0.021). In the antrum of stomach carcinoma, GST-pi expression was positively correlated with the expression of EGFR, TOPOII, and Her-2/Neu, however, it was negatively correlated with the expression of PCNA, and the P27 expression was positively correlated with the P53 expression (r = 0.275, P = 0.042).


CONCLUSIONS
Different molecular mechanisms may lead to the tumorigenesis and development of stomach carcinoma at different sites.
Ye
Yan-wei
YW
Department of Abdominal Surgery, Cancer Hospital, Fudan University, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Shi
Ying-qiang
YQ
Wang
Chun-meng
CM
Li
Bai-zhou
BZ
Zhu
Lei
L
D002299:Cardia / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011706:Pyloric Antrum / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology*