title: Cryptococcus agrionensis sp. nov., a basidiomycetous yeast of the acidic rock drainage ecoclade, isolated from an acidic aquatic environment of volcanic origin.
abstract: Seventy-one strains were isolated from the River Agrio-Lake Caviahue acidic aquatic environment in Argentina. Strains were isolated mainly from the most acidic section of the river (pH 1.8-2.7). According to the mini/microsatellite-primed PCR technique and physiological data, these strains are representatives of a single novel species of the genus Cryptococcus. Analysis of the D1/D2 region of the large-subunit rRNA gene showed that the strains belong to the order Filobasidiales of the subphylum Agaricomycotina. The novel isolates formed a phylogenetic group with Cryptococcus ibericus, Cryptococcus aciditolerans and Cryptococcus metallitolerans, the most closely related species. This group, which is phylogenetically related to the Gastricus clade, is considered to be an ecoclade due to its peculiar ecology and physiology. The name Cryptococcus agrionensis sp. nov. is proposed to accommodate these isolates, with strain CRUB 1317(T) (=CBS 10799(T)=JCM 15321(T)) as the type strain.
authors: Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA-CONICET), Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue (UNCo), Bariloche, Argentina.
authors: Gabriel
authors: G
authors: Russo
authors: Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA-CONICET), Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue (UNCo), Bariloche, Argentina.
authors: Diego
authors: D
authors: Libkind
authors: Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA-CONICET), Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue (UNCo), Bariloche, Argentina.
authors: Ricardo J
authors: RJ
authors: Ulloa
authors: Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA-CONICET), Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue (UNCo), Bariloche, Argentina.
authors: Virginia
authors: V
authors: de García
authors: Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors: Jose P
authors: JP
authors: Sampaio
authors: Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA-CONICET), Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue (UNCo), Bariloche, Argentina.
authors: María R
authors: MR
authors: van Broock
mesh_terms: D001118:Argentina; D003454:Cryptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D018707:Volcanic Eruptions
chemical_list: D004271:DNA, Fungal; D012336:RNA, Ribosomal, 16S