Interfacing Sca-1(pos) mesenchymal stem cells with biocompatible scaffolds with different chemical composition and geometry.
An immortalized murine mesenchymal stem cell line (mTERT-MSC) enriched for Lin(neg)/Sca-1(pos) fraction has been obtained through the transfection of MSC with murine TERT and single-cell isolation. Such cell line maintained the typical MSC self-renewal capacity and continuously expressed MSC phenotype. Moreover, mTERT-MSC retained the functional features of freshly isolated MSC in culture without evidence of senescence or spontaneous differentiation events. Thus, mTERT-MSC have been cultured onto PLA films, 30 and 100 microm PLA microbeads, and onto unpressed and pressed HYAFF-11 scaffolds. While the cells adhered preserving their morphology on PLA films, clusters of mTERT-MSC were detected on PLA beads and unpressed fibrous scaffolds. Finally, mTERT-MSC were not able to colonize the inner layers of pressed HYAFF-11. Nevertheless, such cell line displayed the ability to preserve Sca-1 expression and to retain multilineage potential when appropriately stimulated on all the scaffolds tested.
Forte
G
G
Laboratorio di Cardiologia Molecolare e Cellulare, Dipartimento di Medicina Interna, Università di Roma Tor Vergata, 00133 Roma, Italy.
Franzese
O
O
Pagliari
S
S
Pagliari
F
F
Di Francesco
A M
AM
Cossa
P
P
Laudisi
A
A
Fiaccavento
R
R
Minieri
M
M
Bonmassar
E
E
Di Nardo
P
P
D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D019070:Cell Lineage / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010641:Phenotype; D011108:Polymers / Q000737:chemistry; D019098:Telomerase / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*; D014161:Transduction, Genetic
D000950:Antigens, Ly; D001672:Biocompatible Materials; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D011108:Polymers; D006861:Hydrogen Peroxide; D019098:Telomerase