[The effect of nitric oxide on the proliferation and ALP activity of human dental pulp cells in vitro].
OBJECTIVE
To study the effect of nitric oxide on the biological characteristic of human dental pulp cells.


METHODS
The dental pulp cells were cultured with nitric oxide at different concentration of nitric oxide (10 micromol/L, 50 micromol/L, 100 micromol/L) by tissue explant. The effect of nitric oxide were studied with MTT assay and ALPase immunoassay. The data were statistically analyzed with SPSS13.0 software package.


RESULTS
NOC-18 suppressed the proliferation of pulp cells in a concentration-dependent manner.The pulp cells showed higher ALP activity and NOC-18 promoted the ALP activity in a concentration-dependent manner when cultured in mineralization-promoting medium.While NOC-18 did not affect the ALP activity of cells in the normal medium.


CONCLUSIONS
It is suggested that there is a significant effect of nitric oxide on dental pulp cells proliferation and ALP activity.
Ding
Ji-fen
JF
Shandong Medical College, Linyi 276002, Shandong Province, China. spzh2001@yahoo.com.cn
D000469:Alkaline Phosphatase; D002478:Cells, Cultured; D003782:Dental Pulp; D006801:Humans; D066298:In Vitro Techniques; D009569:Nitric Oxide; D009603:Nitroso Compounds
C093285:NOC 18; D009603:Nitroso Compounds; D009569:Nitric Oxide; D000469:Alkaline Phosphatase