Dielectric constants are not enough: principal component analysis of the influence of solvent properties on molecularly imprinted polymer-ligand rebinding.
The influence of the physical properties of incubation medium on the rebinding of template to bupivacaine molecularly imprinted and non-imprinted methacrylic acid-ethylene dimethacrylate co-polymers has been studied. Principal component analysis (PCA) was employed to identify the factors with the greatest influence on binding. While the dielectric constant (D) made a significant contribution to describing the observed binding, the influence of polarity as reflected in the Snyder polarity index (SPI) was also demonstrated to make a significant contribution. The use of solvents containing hydroxyl functionality in particular was observed to exert unique effects on recognition. The variation in solvent influence on binding at constant D motivates more complex analyses when studying MIP-ligand recognition.
Rosengren
Annika M
AM
Bioorganic & Biophysical Chemistry Laboratory, School of Pure & Applied Natural Sciences, University of Kalmar, SE-391 82 Kalmar, Sweden. annika.rosengren@hik.se
Golker
Kerstin
K
Karlsson
Jesper G
JG
Nicholls
Ian A
IA
D015374:Biosensing Techniques; D002045:Bupivacaine / Q000737:chemistry; D008024:Ligands; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D025341:Principal Component Analysis; D012997:Solvents
D008024:Ligands; D011108:Polymers; D011109:Polymethacrylic Acids; D012997:Solvents; D002045:Bupivacaine