Jeotgalicoccus huakuii sp. nov., a halotolerant bacterium isolated from seaside soil.
A Gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, halotolerant, coccoid bacterium, designated strain NY-2(T), was isolated from a seaside soil sample from Shandong Province, China. Strain NY-2(T) was able to grow in the presence of 0-23 % (w/v) NaCl and at pH 4.5-10.0 and 5-42 degrees C; optimum growth was observed with 3-8 % (w/v) total salts and at pH 6.5-8.0 and 28-37 degrees C. Chemotaxonomic analyses, including fatty acid profiles, menaquinones and polar lipids, supported the affiliation of strain NY-2(T) to the genus Jeotgalicoccus. The predominant menaquinone of strain NY-2(T) was menaquinone 7 (MK-7) (100 %) and the major cellular fatty acids were iso-C(15 : 0) (49.0 %) and anteiso-C(15 : 0) (19.6 %). Cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids. The DNA G+C content of strain NY-2(T) was 36.8 mol%. Based on 16S rRNA gene sequence analysis, strain NY-2(T) formed a coherent cluster with Jeotgalicoccus marinus JSM 076033(T), Jeotgalicoccus halotolerans YKJ-101(T) and Jeotgalicoccus psychrophilus YKJ-115(T). Phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data indicated that strain NY-2(T) (=CCTCC AB 208288(T) =JCM 15687(T)) should be classified as the type strain of a novel species of the genus Jeotgalicoccus, for which the name Jeotgalicoccus huakuii sp. nov. is proposed.
Guo
Xin-Qiang
XQ
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Li
Rong
R
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Zheng
Liu-Qiang
LQ
The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Lin
Dong-Qing
DQ
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Sun
Ji-Quan
JQ
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Li
Shun-Peng
SP
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Jiang
Jian-Dong
JD
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012623:Seawater; D012988:Soil Microbiology; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S