[Immunologic function of palatine tonsil B lymphocyte].
OBJECTIVE
To study cellular immune function of palatine tonsil B lymph cell.


METHODS
The phenotype of palatine tonsil cells (PTC) and that of peripheral blood mononuclear cell (PBMC) were compared using fluorescence staining and flow cytometry (FCM) analysis, then immunomagnetic beads were used to separate CD3- cell in PTC and PBMC. The proliferation function of CD3- lymph cell of PTC and PBMC was tested after stimulated by CD20mAb.


RESULTS
FCM analysis founding that 71.2% PTC express CD20 with higher mean fluorescence intensity, MFI, compared to the 15.5% in PBMC. There's no significant difference between the proliferation of PTC and PBMC B lymph cell.


CONCLUSIONS
CD20 expression is different in PTC and PBMC, but corresponding function is still unknown.
Min
Mike
M
Department of Otolaryngology, No. 117 Hospital of PLA, Hangzhou, 310004, China. meek@263.net
Ma
Chaowu
C
Jin
Boquan
B
Xu
Jianzhong
J
Zhou
Yu
Y
Gan
Xinfei
X
D000328:Adult; D018951:Antigens, CD20 / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology*
D018951:Antigens, CD20