title: Prostaglandin metabolizing enzymes in correlation with vitamin D receptor in benign and malignant breast cell lines.
abstract: BACKGROUND
  The antiproliferative effects of calcitriol (1,25(OH)2D3) mediated via the vitamin D receptor (VDR), render the biologically active form of vitamin D a promising target in breast cancer therapy. Furthermore, breast cancer is associated with inflammatory processes based on an up-regulation of cyclooxygenase-2 (COX-2) expression, the prostaglandin E2 (PGE2) synthesizing enzyme. The PGE2 metabolizing enzyme, 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is described as a tumor suppressor in cancer. First references suggest a correlation between vitamin D and prostaglandin metabolism through the impact of 1,25(OH)2D3 on the expression of COX-2 and 15-PGDH.
  
  
  METHODS
  The expression of VDR, COX-2 and 15-PGDH in benign MCF-10F and malignant MCF-7 breast cells was determined by real-time PCR (RT-PCR) and Western blot analysis.
  
  
  RESULTS
  Although the RT-PCR data were divergent from those obtained from the Western blot analysis, the COX-2 protein expression was MCF-7 2-fold higher in the MCF-7 compared to the MCF-10F cells. Moreover, a correlation of 15-PGDH to VDR by RT-PCR was found in both cell lines. The VDR protein levels were inversely correlated to the 15-PGDH protein levels and revealed that the MCF-10F cells had the highest VDR expression.
  
  
  CONCLUSIONS
  A possible link between VDR-associated target genes and prostaglandin metabolism is suggested.
authors: Department of Obstetrics and Gynecology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany. marc.thill@uk-sh.de
authors: Marc
authors: M
authors: Thill
authors: Dorothea
authors: D
authors: Fischer
authors: Steffi
authors: S
authors: Becker
authors: Tim
authors: T
authors: Cordes
authors: Christine
authors: C
authors: Dittmer
authors: Klaus
authors: K
authors: Diedrich
authors: Darius
authors: D
authors: Salehin
authors: Michael
authors: M
authors: Friedrich
mesh_terms: D001940:Breast / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D018167:Receptors, Calcitriol / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011453:Prostaglandins; D018167:Receptors, Calcitriol; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D051546:Cyclooxygenase 2