title: Wound healing in the jugal mucosa of rats with a cold blade scalpel and an ultrasonic harmonic scalpel.
abstract: Ultrasound harmonic scalpel has been recently introduced in otorhinolaryngological procedures.
  
  
  OBJECTIVE
  to assess macro and microscopic evolution of the healing process of wounds created in the jugal mucosa of rats by the use of ultrasound scalpel.
  
  
  METHODS
  we used 30 Wistar rats in which we made mucosal incisions on the right jugal mucosa with the ultrasound harmonic scalpel (USHS) and on the left side with the cold blade scalpel (CBS). Macroscopic and microscopic evaluations were carried out on the third, seventh and fourteenth days of postoperative. For the microscopic evaluation we used HE to asses the inflammatory process and the Sirius Red approach for collagens type I and III. Anti-CD 3 antibodies and anti-factor VIII assessed the concentration of T-lymphocytes and neovessels.
  
  
  RESULTS
  the USHS caused greater cell damage with reepitelization delay. Microscopy showed more intense inflammatory reactions and a loss in collagen build up, delay in scar maturation and a greater vessel neoformation.
  
  
  CONCLUSIONS
  USHS brings about a greater lesion in the incision area; delayed regeneration; promotes greater inflammatory process and angiogenic activity; delays in fibroplasia and scar tissue maturation on the rats' jugal mucosa when compared to cold blade scalpel.
authors: Federal University of Paraná.
authors: Elen Carolina David João
authors: EC
authors: De Masi
authors: Sergio Luis
authors: SL
authors: Rocha
authors: Marcos
authors: M
authors: Mocellin
authors: João Luis Garcia de
authors: JL
authors: Faria
mesh_terms: D000818:Animals; D005260:Female; D006488:Hemostasis, Surgical / Q000295:instrumentation; D009061:Mouth Mucosa / Q000473:pathology / Q000601:surgery*; D011446:Prospective Studies; D051381:Rats; D017208:Rats, Wistar; D013525:Surgical Instruments; D014464:Ultrasonic Therapy / Q000295:instrumentation*; D014945:Wound Healing