title: Intracellular polysilicon barcodes for cell tracking.
abstract: During the past decade, diverse types of barcode have been designed in order to track living cells in vivo or in vitro, but none of them offer the possibility to follow an individual cell up to ten or more days. Using silicon microtechnologies a barcode sufficiently small to be introduced into a cell, yet visible and readily identifiable under an optical microscope, is designed. Cultured human macrophages are able to engulf the barcodes due to their phagocytic ability and their viability is not affected. The utility of the barcodes for cell tracking is demonstrated by following individual cells for up to ten days in culture and recording their locomotion. Interestingly, silicon microtechnology allows the mass production of reproducible codes at low cost with small features (bits) in the micrometer range that are additionally biocompatible.
authors: Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, 08193-Bellaterra, Barcelona, Spain.
authors: Elisabet
authors: E
authors: Fernandez-Rosas
authors: Rodrigo
authors: R
authors: Gómez
authors: Elena
authors: E
authors: Ibañez
authors: Leonardo
authors: L
authors: Barrios
authors: Marta
authors: M
authors: Duch
authors: Jaume
authors: J
authors: Esteve
authors: Carme
authors: C
authors: Nogués
authors: José Antonio
authors: JA
authors: Plaza
mesh_terms: D002478:Cells, Cultured; D001330:Electronic Data Processing; D005240:Feasibility Studies; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D012825:Silicon / Q000737:chemistry*
chemical_list: D012825:Silicon