title: Improving administration regimens of CyaA-based vaccines using TRAP assays to detect antigen-specific CD8(+) T cells directly ex vivo.
abstract: Vaccination with recombinant adenylate cyclase of Bordetella pertussis (CyaA) carrying antigen is a promising approach to target antigen-presenting cells. We have used Trogocytosis Analysis Protocol (TRAP) assays to monitor immune responses raised by different vaccination regimens with recombinant CyaA carrying the ovalbumin antigen. We find that the intradermal, intramuscular or subcutaneous routes are all superior to intravenous injections, and actually lead to a sufficiently high frequency of reactive CTL to be detected and characterized directly ex vivo by TRAP assay or other standard assays. Finally, for all routes, we find a clear boosting effect upon re-injection of the vaccine.
authors: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), F-31077 Toulouse, France.
authors: Sandrine
authors: S
authors: Daubeuf
authors: Xavier
authors: X
authors: Préville
authors: Marie
authors: M
authors: Momot
authors: Yolande
authors: Y
authors: Misseri
authors: Etienne
authors: E
authors: Joly
authors: Denis
authors: D
authors: Hudrisier
mesh_terms: D037361:Adenylate Cyclase Toxin / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D037361:Adenylate Cyclase Toxin; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D010047:Ovalbumin