Light scattering characterization of mitochondrial aggregation in single cells.
Three dimensional finite-difference time-domain (FDTD) simulations are employed to show that light scattering techniques may be used to infer the mitochondrial distributions that exist within single biological cells. Two-parameter light scattering plots of the FDTD light scattering spectra show that the small angle forward scatter can be used to differentiate the case of a random distribution of mitochondria within a cell model from that in which the mitochondria are aggregated to the nuclear periphery. Fourier transforms of the wide angle side scatter spectra show a consistent highest dominant frequency, which may be used for size differentiation of biological cells with distributed mitochondria.
Su
Xuan-Tao
XT
Department of Physics, University of Alberta, Edmonton T6G 2G7, Canada. xtsu@phys.ualberta.ca
Singh
Kirat
K
Rozmus
Wojciech
W
Backhouse
Christopher
C
Capjack
Clarence
C
D002449:Cell Aggregation / Q000502:physiology; D002452:Cell Count / Q000379:methods*; D003198:Computer Simulation; D008027:Light; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure*; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012542:Scattering, Radiation