[Screening study of the kinetogenic effects of serum containing four Chinese materia medicas on the colonic smooth muscle cells in rats].
OBJECTIVE
To study the kinetogenic effects of serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala on the colonic smooth muscle cells of rats.


METHODS
Serum containing Chinese materia medicas was made according to standard methods. Smooth muscle cells were isolated from the muscle layers of Wistar rat's colon, referred to modified Bitar's method. The contractile response of colonic smooth muscle cells to serum containing Chinese materia medicas (10%, 50%, 100% concentration) and other medicines (blank and 1 x 10(-3) mol/L acetylcholine) were separately observed. The contractility was presented by the decrease of the cell length between the drug groups and the control.


RESULTS
Serum containing each Chinese materia medica can make dose-dependent contraction at different concentrations (P < 0.05), but the strongest effect of each serum had no significant difference (P > 0.05).


CONCLUSIONS
Serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala can make notable contraction on colonic smooth muscle cells in rats.
Shi
Yan-Ting
YT
The Second Affiliated Hospital of Shandong University, Jinan. jianqiangg@sdu.edu.cn
Si
Chun-Feng
CF
Liu
Bin
B
D000818:Animals; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D051381:Rats
D004365:Drugs, Chinese Herbal