Protective effect on enamel demineralization of a CPP-ACP paste: an AFM in vitro study.
OBJECTIVE
The aim of the present in vitro study was the evaluation of a CPP-ACP paste (Tooth Mousse) on preventing dental erosion produced by a soft drink (Coca Cola), using Atomic Force Microscopy (AFM).


METHODS
Thirty extracted human central incisors free of caries were selected and divided in a treatment and a control half. The treatment halves were divided in three groups-group 1: demineralization with soft drink (4 intervals of 2 min); group 2: demineralization with soft drink (4 intervals of 2 min) plus Tooth Mousse; group 3: intact enamel plus Tooth Mousse. In groups 2 and 3 Tooth Mousse was applied for 3 min at 0, 8, 24 and 36 h. The surface of each specimen was imaged by AFM and R(rms) values were registered.


RESULTS
Among treatment specimens of groups 1 and 2, a statistically significant difference (P<0.01) in R(rms) values was registered: treatment of the specimens with CPP-ACP paste had a protective effect on enamel demineralization. In group 3 no statistically significant difference was registered between exposed and not exposed halves of the specimens.


CONCLUSIONS
The use of a CPP-ACP paste had a protective effect on enamel demineralization in an in vitro model.
Poggio
Claudio
C
Department of Operative Dentistry, University of Pavia, Piazzale Golgi 3, Pavia, Italy. nadine.schlueter@dentist.med.uni-giessen.de
Lombardini
Marco
M
Dagna
Alberto
A
Chiesa
Marco
M
Bianchi
Stefano
S
D002253:Carbonated Beverages / Q000009:adverse effects; D002364:Caseins / Q000627:therapeutic use*; D003743:Dental Enamel / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D020011:Protective Agents / Q000627:therapeutic use*; D013696:Temperature; D013997:Time Factors; D017001:Tooth Demineralization / Q000517:prevention & control*; D014077:Tooth Erosion / Q000517:prevention & control*; D014089:Tooth Remineralization / Q000379:methods
D002364:Caseins; D020011:Protective Agents; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex