title: Microarray analysis of MCF-7 breast cancer cells treated with 1,25-dihydroxyvitamin D3 or a 17-methyl-D-ring analog.
abstract: BACKGROUND
  1alpha,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] is the biological active form of vitamin D. Its antiproliferative capacities make it a potential drug to treat diseases such as cancer. The clinical use of 1,25-(OH)2D3 as an antiproliferative agent is hampered by its calcemic effects. Hence, structural analogs such as the seco-9,11-bisnor-17-methyl analog, WY1112, have been developed with superagonistic capacities. This study aims to distinct the molecular activities of 1,25-(OH)2D3 and WY1112 and identify possible differences in gene expression.
  
  
  METHODS
  Total RNA was extracted from MCF-7 breast cancer cells treated with 1,25-(OH)2D3 or WY1112 and was used for microarray analysis.
  
  
  RESULTS
  The experiments revealed that WY1112 induces the same genes as 1,25-(OH)2D3, but the induction level of the individual genes is higher. Microarray analysis did not reveal genes that were exclusively regulated by WY1112.
  
  
  CONCLUSIONS
  The superagonistic vitamin D analog WY1112 induces the same set of genes as 1,25-(OH)2D3, but the level of induction of the individual genes is higher.
authors: Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Katholieke Universiteit Leuven, Gasthuisberg, 3000 Leuven, Belgium.
authors: Els
authors: E
authors: Vanoirbeek
authors: Guy
authors: G
authors: Eelen
authors: Lieve
authors: L
authors: Verlinden
authors: Kathleen
authors: K
authors: Marchal
authors: Kristof
authors: K
authors: Engelen
authors: Bart
authors: B
authors: De Moor
authors: Ine
authors: I
authors: Beullens
authors: Suzanne
authors: S
authors: Marcelis
authors: Pierre
authors: P
authors: De Clercq
authors: Roger
authors: R
authors: Bouillon
authors: Annemieke
authors: A
authors: Verstuyf
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D002117:Calcitriol / Q000031:analogs & derivatives / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D002117:Calcitriol