Allosteric modulation of DNA by small molecules.
Many human diseases are caused by dysregulated gene expression. The oversupply of transcription factors may be required for the growth and metastatic behavior of human cancers. Cell permeable small molecules that can be programmed to disrupt transcription factor-DNA interfaces could silence aberrant gene expression pathways. Pyrrole-imidazole polyamides are DNA minor-groove binding molecules that are programmable for a large repertoire of DNA motifs. A high resolution X-ray crystal structure of an 8-ring cyclic Py/Im polyamide bound to the central 6 bp of the sequence d(5'-CCAGGCCTGG-3')2 reveals a 4 A widening of the minor groove and compression of the major groove along with a >18 degrees bend in the helix axis toward the major groove. This allosteric perturbation of the DNA helix provides a molecular basis for disruption of transcription factor-DNA interfaces by small molecules, a minimum step in chemical control of gene networks.
Chenoweth
David M
DM
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Dervan
Peter B
PB
D000494:Allosteric Regulation / Q000187:drug effects; D019943:Amino Acid Substitution; D001483:Base Sequence; D018360:Crystallography, X-Ray; D003500:Cyclization; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009757:Nylons / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
D009757:Nylons; D014867:Water; D004247:DNA