Intercellular communication of cellular stress monitored by gamma-H2AX induction.
When cells are exposed to ionizing radiation (IR), unexposed cells that share media with damaged cells exhibit similar effects to irradiated cells including increased levels of DNA double-strand breaks (DSBs). Hypothesizing that this effect, known as the radiation-induced bystander effect, may be a specific instance of communication between damaged and undamaged cells regardless of damage source, we demonstrated that exposure of target cells to non-IR induces bystander damage in non-targeted cells as measured by gamma-H2AX and 53BP1 focal formation. Initially, bystander damage was found primarily in S-phase cells, but at later times, non-S-phase cells were also affected. In addition, media from undamaged malignant and senescent cells also was found to induce DSBs in primary cultures. Media conditioned on cells targeted with either ionizing or non-IR as well as on undamaged malignant and senescent cells contained elevated levels of several cytokines. One of these, transforming growth factor beta (TGF-beta), and nitric oxide (NO) were found to elevate numbers of gamma-H2AX/53BP1 foci in normal cell cultures similar to levels found in bystander cells, and this elevation was abrogated by NO synthase inhibitors, TGF-beta blocking antibody and antioxidants. These findings support the hypothesis that damage in bystander cells results from their exposure to cytokines or reactive compounds released from stressed cells, regardless of damage source. These results have implications for oncogenesis in that they indicate that damaged normal cells or undamaged tumor cells may induce genomic instability, leading to an increased risk of oncogenic transformation in other cells with which they share media or contact directly.
Dickey
Jennifer S
JS
Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20952, USA. dickeyj@mail.nih.gov
Baird
Brandon J
BJ
Redon
Christophe E
CE
Sokolov
Mykyta V
MV
Sedelnikova
Olga A
OA
Bonner
William M
WM
D001940:Breast / Q000166:cytology / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006367:HeLa Cells / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009569:Nitric Oxide / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D016196:S Phase; D013312:Stress, Physiological / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms / Q000235:genetics
D004273:DNA, Neoplasm; D004791:Enzyme Inhibitors; C468783:H2AX protein, human; D006657:Histones; D016212:Transforming Growth Factor beta; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase