Acidocella aluminiidurans sp. nov., an aluminium-tolerant bacterium isolated from Panicum repens grown in a highly acidic swamp in actual acid sulfate soil area of Vietnam.
An aluminium-tolerant bacterium, strain AL46(T), was isolated from a waterweed, Panicum repens, grown in a highly acidic swamp (pH 3) at an actual acid sulfate soil area of Vietnam. Cells were Gram-negative, aerobic, non-spore-forming, non-motile rods (0.3 microm wide and 1.2-1.6 microm long). 16S rRNA gene sequence analysis indicated that strain AL46(T) belongs to the genus Acidocella, class Alphaproteobacteria. Strain AL46(T) was related most closely to the type strains of Acidocella facilis and Acidocella aminolytica (99.4 and 97.8 % 16S rRNA gene sequence similarity, respectively). Levels of DNA-DNA relatedness between strain AL46(T) and the above type strains were 40 %. The results of physiological and biochemical tests allowed the novel strain to be differentiated phenotypically from the two recognized Acidocella species. Data for predominant cellular fatty acids (cyclopropyl C(19 : 0) and C(18 : 1)), major isoprenoid quinone (Q-10) and DNA G+C content (65.6 mol%) were in accordance with those reported for the genus Acidocella. Therefore, strain AL46(T) is considered to represent a novel species of the genus Acidocella, for which the name Acidocella aluminiidurans sp. nov. is proposed. The type strain is AL46(T) (=NBRC 104303(T) =VTCC-D9-1(T)).
Kimoto
Ken-Ichiro
KI
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Aizawa
Tomoko
T
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Urai
Makoto
M
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Bao Ve
Nguyen
N
Department of Crop Science, College of Agriculture, Can Tho University, Campus II, 3/2 Street, Can Tho, Vietnam.
Suzuki
Ken-Ichiro
KI
Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0812, Japan.
Nakajima
Mutsuyasu
M
Environmental Program, Nihon University Advanced Research Institute for the Sciences and Humanities, 12-5 Goban-cho Chiyoda, Tokyo 102-8251, Japan.|Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Sunairi
Michio
M
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
D016947:Acetobacteraceae / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000535:Aluminum / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D008897:Panicum / Q000254:growth & development / Q000382:microbiology; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis*; D013045:Species Specificity; D013431:Sulfates / Q000032:analysis; D014744:Vietnam; D053833:Wetlands
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D013431:Sulfates; D000535:Aluminum