title: Evaluation of genetic diversity among 'Candidatus Liberibacter asiaticus' isolates collected in Southeast Asia.
abstract: The aim of this study was to investigate the genetic diversity and relationships among 'Candidatus Liberibacter asiaticus' isolates from different hosts and distinct geographical areas in Southeast Asia. Genetic diversity among 'Ca. Liberibacter asiaticus' was estimated by sequencing four well-characterized DNA fragments: the 16S ribosomal DNA (rDNA) and 16S/23S intergenic spacer regions; the outer membrane protein (omp) gene region; the trmU-tufB-secE-nusG-rplKAJL-rpoB region (gene cluster region); and the bacteriophage-type DNA polymerase region. The sequences of the 16S rDNA and 16S/23S intergenic spacer regions were identical among all 'Ca. Liberibacter asiaticus' isolates. In contrast, nucleotide substitutions were observed in both the omp gene and the gene cluster regions. However, extended bacteriophage-type DNA polymerase sequences acquired by thermal asymmetric interlaced polymerase chain reaction provided the most sequence diversity among isolates. Phylogenetic analysis of the bacteriophage-type DNA polymerase sequences revealed three clusters in the Southeast Asian 'Ca. Liberibacter asiaticus' population. All Indonesian 'Ca. Liberibacter asiaticus' isolates clustered in one group. The other clusters were not correlated with geographic distribution. The differences in genetic sequences did not reflect differences in the original citrus host (mandarin or pummelo). These results suggest that the bacteriophage-type DNA polymerase region would be useful for molecular differentiation between different Southeast Asian 'Ca. Liberibacter asiaticus' isolates.
authors: Kuchinotsu Citrus Research Station, National Institute of Fruit Tree Science, Kuchinotsu, Minami-shimabara, Nagasaki 859-2501, Japan.
authors: Kenta
authors: K
authors: Tomimura
authors: Shin-Ichi
authors: S
authors: Miyata
authors: Noriko
authors: N
authors: Furuya
authors: Kenji
authors: K
authors: Kubota
authors: Mitsuru
authors: M
authors: Okuda
authors: Siti
authors: S
authors: Subandiyah
authors: Ting-Hsuan
authors: TH
authors: Hung
authors: Hong-Ji
authors: HJ
authors: Su
authors: Toru
authors: T
authors: Iwanami
mesh_terms: D001210:Asia, Southeastern; D001483:Base Sequence; D017931:DNA Primers; D014644:Genetic Variation; D008969:Molecular Sequence Data; D020560:Proteobacteria / Q000145:classification*; D013045:Species Specificity
chemical_list: D017931:DNA Primers