title: AMP-activated protein kinase mediates the interferon-gamma-induced decrease in intestinal epithelial barrier function.
abstract: Impaired epithelial barrier function plays a crucial role in the pathogenesis of inflammatory bowel disease. Elevated levels of the pro-inflammatory cytokine, interferon-gamma (IFNgamma), are believed to be prominently involved in the pathogenesis of Crohn disease. Treatment of T(84) intestinal epithelial cells with IFNgamma severely impairs their barrier properties measured as transepithelial electrical resistance (TER) or permeability and reduces the expression of tight junction proteins such as occludin and zonula occludens-1 (ZO-1). However, little is known about the signaling events that are involved. The cellular energy sensor, AMP-activated protein kinase (AMPK), is activated in response to cellular stress, as occurs during inflammation. The aim of this study was to investigate a possible role for AMPK in mediating IFNgamma-induced effects on the intestinal epithelial barrier. We found that IFNgamma activates AMPK by phosphorylation, independent of intracellular energy levels. Inhibition of AMPK prevents, at least in part, the IFNgamma-induced decrease in TER. Furthermore, AMPK knockdown prevented the increased epithelial permeability, the decreased TER, and the decrease in occludin and ZO-1 caused by IFNgamma treatment of T(84) cells. However, AMPK activity alone was not sufficient to cause alterations in epithelial barrier function. These data show a novel role for AMPK, in concert with other signals induced by IFNgamma, in mediating reduced epithelial barrier function in a cell model of chronic intestinal inflammation. These findings may implicate AMPK in the pathogenesis of chronic intestinal inflammatory conditions, such as inflammatory bowel disease.
authors: Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, California 92093.
authors: Michael
authors: M
authors: Scharl
authors: Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, California 92093.
authors: Gisela
authors: G
authors: Paul
authors: Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, California 92093.
authors: Kim E
authors: KE
authors: Barrett
authors: Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, California 92093. Electronic address: dmccole@ucsd.edu.
authors: Declan F
authors: DF
authors: McCole
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology; D002478:Cells, Cultured; D017097:Electric Impedance; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000503:physiopathology; D007371:Interferon-gamma / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000473:pathology / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D062793:Occludin; D018408:Patch-Clamp Techniques; D010750:Phosphoproteins / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D062826:Zonula Occludens-1 Protein
chemical_list: D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; D010750:Phosphoproteins; D034741:RNA, Small Interfering; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D007371:Interferon-gamma; D055372:AMP-Activated Protein Kinases