Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.).
Eggplant (Solanum melongena L.), also known as aubergine or brinjal, is an important vegetable in many countries. Few useful molecular markers have been reported for eggplant. We constructed simple sequence repeat (SSR)-enriched genomic libraries in order to develop SSR markers, and sequenced more than 14,000 clones. From these sequences, we designed 2,265 primer pairs to flank SSR motifs. We identified 1,054 SSR markers from amplification of 1,399 randomly selected primer pairs. The markers have an average polymorphic information content of 0.27 among eight lines of S. melongena. Of the 1,054 SSR markers, 214 segregated in an intraspecific mapping population. We constructed cDNA libraries from several eggplant tissues and obtained 6,144 expressed sequence tag (EST) sequences. From these sequences, we designed 209 primer pairs, 7 of which segregated in the mapping population. On the basis of the segregation data, we constructed a linkage map, and mapped the 236 segregating markers to 14 linkage groups. The linkage map spans a total length of 959.1 cM, with an average marker distance of 4.3 cM. The markers should be a useful resource for qualitative and quantitative trait mapping and for marker-assisted selection in eggplant breeding.
Nunome
Tsukasa
T
National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Organization (NARO), 360 Kusawa, Ano, Tsu, Mie 514-2392, Japan. nunome@affrc.go.jp
Negoro
Satomi
S
Kono
Izumi
I
Kanamori
Hiroyuki
H
Miyatake
Koji
K
Yamaguchi
Hirotaka
H
Ohyama
Akio
A
Fukuoka
Hiroyuki
H
D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D015698:Genomic Library; D018895:Microsatellite Repeats; D018598:Minisatellite Repeats; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic; D032323:Solanum melongena / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers