Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor.
BACKGROUND
In response to pathogen attack, grapevine synthesizes phytoalexins belonging to the family of stilbenes. Grapevine cell cultures represent a good model system for studying the basic mechanisms of plant response to biotic and abiotic elicitors. Among these, modified beta-cyclodextrins seem to act as true elicitors inducing strong production of the stilbene resveratrol.


RESULTS
The transcriptome changes of Vitis riparia x Vitis berlandieri grapevine cells in response to the modified beta-cyclodextrin, DIMEB, were analyzed 2 and 6 h after treatment using a suppression subtractive hybridization experiment and a microarray analysis respectively. At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases. At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.


CONCLUSIONS
We report the first large-scale study of the molecular effects of DIMEB, a resveratrol inducer, on grapevine cell cultures. This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.
Zamboni
Anita
A
IASMA Research and Innovation Center, Fondazione Edmund Mach, S. Michele a/Adige I-38010, Italy. anita.zamboni@univr.it
Gatto
Pamela
P
Cestaro
Alessandro
A
Pilati
Stefania
S
Viola
Roberto
R
Mattivi
Fulvio
F
Moser
Claudio
C
Velasco
Riccardo
R
D002455:Cell Division; D002473:Cell Wall / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D018749:RNA, Plant / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000494:pharmacology*
D018076:DNA, Complementary; D018749:RNA, Plant; D013267:Stilbenes; D047392:beta-Cyclodextrins; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin; D000077185:Resveratrol