title: A rapidly resorbable hemostatic biomaterial based on dihydroxyacetone.
abstract: We have developed a rapid acting, rapidly resorbable, non-toxic, topical hemostatic agent comprised of a PEGylated, polymerized sequence of dihydroxyacetone (MPEG-pDHA) that is highly effective in vivo. Twenty-eight Sprague-Dawley rats underwent left lateral hepatectomy. To the cut edge of the liver, rats received MPEG-pDHA (50 mg), normal saline (0.5 mL), or Instat (50 mg), a commercially available hemostatic compound. Bleeding time and total blood loss were quantified. Coagulation studies and scanning electron microscopy were performed on phlebotomized blood combined with MPEG-pDHA. Rats treated with MPEG-pDHA had significantly decreased bleeding time (97 s) and total blood loss (1.35 g) compared to normal saline (464 s and 3.83 g, p < 0.05 for each), and a significantly shorter bleeding time compared to Instat (165 s, p < 0.05). Histology confirmed that all MPEG-pDHA was metabolized within 3 weeks. The addition of MPEG-pDHA to whole blood did not significantly affect prothrombin time (12.0 s vs. 13.2 s, p = 0.130), partial thromboplastin time (27.0 s vs. 21.8 s, p = 0.118), or thrombin clotting time. MPEG-pDHA is an effective and rapidly resorbable hemostatic agent that may find broad hemostatic application in a wide range of surgical procedures.
authors: Division of Plastic and Reconstructive Surgery, Laboratory for Bioregenerative Medicine and Surgery, Weill Cornell Medical College, New York, New York, USA.
authors: Peter W
authors: PW
authors: Henderson
authors: Daniel J M
authors: DJ
authors: Kadouch
authors: Sunil P
authors: SP
authors: Singh
authors: Peter N
authors: PN
authors: Zawaneh
authors: Jennifer
authors: J
authors: Weiser
authors: Sara
authors: S
authors: Yazdi
authors: Andrew
authors: A
authors: Weinstein
authors: Ursula
authors: U
authors: Krotscheck
authors: Bennett
authors: B
authors: Wechsler
authors: David
authors: D
authors: Putnam
authors: Jason A
authors: JA
authors: Spector
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001780:Blood Coagulation Tests; D004098:Dihydroxyacetone / Q000737:chemistry / Q000378:metabolism; D006490:Hemostatics / Q000737:chemistry / Q000378:metabolism; D006498:Hepatectomy; D008099:Liver / Q000293:injuries; D008297:Male; D008422:Materials Testing; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001672:Biocompatible Materials; D006490:Hemostatics; D011092:Polyethylene Glycols; D004098:Dihydroxyacetone