Performance characteristics of beta-2-microglobulin measurements on a Beckman Immage analyzer with the DakoCytomation reagent kit.
OBJECTIVE
Evaluation of serum beta-2-microglobulin (beta2M) in an automated analyzer.


METHODS
The DakoCytomation beta2M kit is an antibody based reagent intended for quantitative determination of beta2M in serum and plasma by rate nephelometry.


RESULTS
The limit of blank is 0.16 mg/L. The method is linear up to 17.9 mg/L. The imprecision ranged from 2.1% to 7.9% at the concentrations of 1.77 and 7.19 mg/L, respectively. Method comparison yielded slope=1.009, r=0.998. No interference was observed from hemolytic or icteric specimens. Reference interval of a healthy population was 1.13 mg/L to 3.04 mg/L.


CONCLUSIONS
The DakoCytomation reagent is acceptable to measure serum beta2M.
De
Barun K
BK
Department of Pathology, University of Arizona Health Sciences Center, Tucson, AZ 85724-5059, USA. bkde@email.arizona.edu
Jimenez
Ernest
E
De
Soumo
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult; D001613:beta 2-Microglobulin / Q000032:analysis*
D011933:Reagent Kits, Diagnostic; D001613:beta 2-Microglobulin