title: Simultaneous quantitative analysis of N-acylethanolamides in clinical samples.
abstract: A simple and rapid analytical method is described for the simultaneous quantitative analysis of three different N-acylethanolamides in human biological samples: anandamide (AEA), oleoylethanolamide (OEA), and palmitoylethanolamide (PEA). The method is based on a new hybrid solid phase extraction-precipitation technology followed by ultra-performance liquid chromatography/mass spectrometry (UPLC/MS) analysis using d(4)-AEA as the internal standard. The method is linear up to 100ng/ml with a limit of quantitation of 50pg/ml for AEA and 100pg/ml for OEA and PEA. Good reproducibility, accuracy, and precision were demonstrated during the method validation. Application of this new methodology to the analysis of clinical study samples is presented.
authors: Exploratory Development Department, Astellas Pharma Global Development Europe, AC Leiderdorp, The Netherlands.
authors: Aliye
authors: A
authors: Ozalp
authors: Begona
authors: B
authors: Barroso
mesh_terms: D000293:Adolescent; D000328:Adult; D000577:Amides; D000581:Amidohydrolases / Q000037:antagonists & inhibitors; D001095:Arachidonic Acids / Q000032:analysis* / Q000097:blood; D002138:Calibration; D002186:Cannabinoids; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D063388:Endocannabinoids; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D004983:Ethanolamines; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D009829:Oleic Acids / Q000032:analysis* / Q000097:blood; D010169:Palmitic Acids / Q000032:analysis* / Q000097:blood; D053284:Polyunsaturated Alkamides / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000577:Amides; D000581:Amidohydrolases; D001095:Arachidonic Acids; D002186:Cannabinoids; D063388:Endocannabinoids; D004791:Enzyme Inhibitors; D004983:Ethanolamines; D009829:Oleic Acids; D010169:Palmitic Acids; D053284:Polyunsaturated Alkamides; D000099252:Fatty Acid Amide Hydrolases; C456739:virodhamine; C488250:oleoylethanolamide; C005958:palmidrol; C078814:anandamide