Gallaecimonas pentaromativorans gen. nov., sp. nov., a bacterium carrying 16S rRNA gene heterogeneity and able to degrade high-molecular-mass polycyclic aromatic hydrocarbons.
A Gram-negative, rod-shaped, halotolerant bacterium, designated strain CEE_131(T), which degraded high-molecular-mass polycyclic aromatic hydrocarbons of four and five rings, was isolated from intertidal sediment of Corcubion Ria in Cee, A Coruña, Spain. Direct sequencing showed ambiguities and suggested heterogeneity. Cloned 16S rRNA gene sequence PCR products yielded five different sequences varying at five positions. Strain CEE_131(T) showed rather distant relationships to its phylogenetically closest neighbours, including the genera Rheinheimera and Serratia , exhibiting 91 % sequence similarity with Rheinheimera perlucida BA131(T) and Serratia proteamaculans subsp. quinovora DSM 4597(T). The major fatty acids were C(16 : 1 )omega7c, C(16 : 0) and C(18 : 1)omega7c. The DNA G+C content was 41.7 mol%. On the basis of these distinct phenotypic and genotypic characteristics, strain CEE_131(T) is considered to represent a novel species in a new genus in the class Gammaproteobacteria, for which the name Gallaecimonas pentaromativorans gen. nov., sp. nov. is proposed. The type strain is CEE_131(T) (=DSM 21945(T)=CECT 7479(T)).
Rodríguez-Blanco
Arturo
A
CNRS, UMR7621, Laboratoire d'Océanographie Biologique de Banyuls, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.|UPMC Univ Paris 06, UMR 7621, Laboratoire ARAGO, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.
Vetion
Gilles
G
CNRS, UMR7621, Laboratoire d'Océanographie Biologique de Banyuls, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.|UPMC Univ Paris 06, UMR 7621, Laboratoire ARAGO, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.
Escande
Marie-Line
ML
CNRS, UMR7621, Laboratoire d'Océanographie Biologique de Banyuls, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.|UPMC Univ Paris 06, UMR 7621, Laboratoire ARAGO, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.
Delille
Daniel
D
CNRS, UMR7621, Laboratoire d'Océanographie Biologique de Banyuls, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.|UPMC Univ Paris 06, UMR 7621, Laboratoire ARAGO, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.
Ghiglione
Jean-François
JF
CNRS, UMR7621, Laboratoire d'Océanographie Biologique de Banyuls, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.|UPMC Univ Paris 06, UMR 7621, Laboratoire ARAGO, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013030:Spain
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S