Relationship between two estrogen receptor-alpha gene polymorphisms and angiographic coronary artery disease.
OBJECTIVE
To investigate the association of estrogen receptor-alpha PvuII and BtgI polymorphisms with angiographic presence and severity of coronary artery disease (CAD).


METHODS
Our cross-sectional study included 140 patients with >or=50% coronary stenoses (CAD group) and 47 patients with normal angiograms (CAD-free group) (total n=187, age 59.6+/- 13.2 years; 66 women). PvuII and BtgI genotype and allele distributions were determined by standard method of polymerase chain reaction and restriction fragment length polymorphism. The CAD subgroups by the number of diseased vessels were also defined. Variable associations and group differences were analyzed by independent t test, one-way ANOVA, Pearson's Chi-square, Spearman's correlation tests and logistic regression analyses.


RESULTS
While there was no association between PvuII polymorphism and angiographic CAD (p=0.384), BtgI polymorphism was more prevalent in CAD-free group (23.4% vs. 10% (CAD group), OR=2.75, 95% CI=1.150 to 6.579, p=0.019). This difference was more pronounced in women (28.6% vs. 4.4%; OR=8.6; 95% CI=1.564 to 47.303; p=0.005) compared to men (p=0.391). Logistic regression analysis confirmed BtgI polymorphism as the most important predictor for a normal coronary angiogram among parameters such as body mass index, diabetes and age (OR 8.13, 95% CI 1.257 to 52.627, p=0.028). However, no significant association between BtgI polymorphism and the number of stenotic arteries was detected.


CONCLUSIONS
ESR1 PvuII polymorphism is not associated with angiographically significant CAD. ESR1 BtgI polymorphism is strongly associated with the presence of normal coronary angiograms in women, which suggests protective effect. Further confirmation of these findings is required.
Karadağ
Bilgehan
B
Department of Cardiology, Cerrahpaşa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Güven
Mehmet
M
Hacioğlu
Yalçin
Y
Oz
Erdinç
E
Batar
Bahadir
B
Domaniç
Nergiz
N
Ulutin
Turgut
T
Vural
Vural Ali
VA
D016022:Case-Control Studies; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging* / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D003430:Cross-Sectional Studies; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D012737:Sex Factors
C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha