title: Sera from anti-Jo-1-positive patients with polymyositis and interstitial lung disease induce expression of intercellular adhesion molecule 1 in human lung endothelial cells.
abstract: OBJECTIVE
  To investigate whether sera or purified IgG from patients with polymyositis (PM) and patients with dermatomyositis (DM), with or without interstitial lung disease (ILD), can activate endothelial cells (ECs).
  
  
  METHODS
  Patients' sera were selected based on the presence or absence of anti-Jo-1, anti-SSA, or anti-U1 small nuclear RNP autoantibodies. The presence of autoantibodies was determined by line blot assays. Cultured human microvascular ECs derived from lung tissue (HMVEC-L) were incubated with sera or purified IgG from 22 patients with PM, 7 patients with DM, and 10 healthy individuals as controls. Assessment of intercellular adhesion molecule 1 (ICAM-1) expression was conducted by immunofluorescence (n=22) and by cell-based enzyme-linked immunosorbent assay (ELISA) (n=20). Serum levels of soluble ICAM-1 (sICAM-1) were determined by ELISA.
  
  
  RESULTS
  Sera from PM patients with ILD who were positive for anti-Jo-1 autoantibodies had a significantly stronger effect on the expression of ICAM-1 by HMVEC-L in comparison with sera from healthy controls and patients with other autoantibodies. Purified IgG did not induce ICAM-1 expression. Higher serum levels of sICAM-1 were found in patients with myositis compared with healthy controls.
  
  
  CONCLUSIONS
  EC activation with ICAM-1 expression could contribute to the multiorgan involvement, including the development of myositis and ILD, in patients carrying anti-Jo-1 autoantibodies. The EC-activating factors are not the autoantibodies themselves, but might be systemic factors associated with these autoantibodies.
authors: Karolinska University Hospital Solna, Rheumatology Unit, Karolinska Institutet, Stockholm, Sweden. Sevim.Barbasso@ki.se
authors: Sevim
authors: S
authors: Barbasso Helmers
authors: Pernilla
authors: P
authors: Englund
authors: Marianne
authors: M
authors: Engström
authors: Erik
authors: E
authors: Ahlin
authors: Maryam
authors: M
authors: Fathi
authors: Sabina
authors: S
authors: Janciauskiene
authors: Mikael
authors: M
authors: Heimbürger
authors: Johan
authors: J
authors: Rönnelid
authors: Ingrid E
authors: IE
authors: Lundberg
mesh_terms: D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000097:blood* / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006643:Histidine-tRNA Ligase / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology; D007074:Immunoglobulin G / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood / Q000378:metabolism*; D008168:Lung / Q000098:blood supply; D017563:Lung Diseases, Interstitial / Q000097:blood* / Q000276:immunology; D008297:Male; D055806:Microvessels / Q000166:cytology; D008875:Middle Aged; D017285:Polymyositis / Q000097:blood* / Q000276:immunology; D055815:Young Adult
chemical_list: D000974:Antibodies, Antinuclear; D007106:Immune Sera; D007074:Immunoglobulin G; C046750:Jo-1 antibody; D018799:Intercellular Adhesion Molecule-1; D006643:Histidine-tRNA Ligase