Fingerprinting alterations of secondary metabolites of tangerine peels during growth by HPLC-DAD and chemometric methods.
Tangerine peels are herbal materials of two coupled traditional Chinese medicines, Pericarpium Citri Reticulatae (PCR) and Pericarpium Citri Reticulatae Viride (PCRV). In this paper, high-performance liquid chromatographic fingerprints of tangerine peels during growth were firstly measured for deliberately collected 34 samples from three species (Citrus reticulata 'Chachi', Citrus reticulata 'Dahongpao' and Citrus erythrosa Tanaka). After sixteen characteristic components which have similar change trends in the grown process were screened out with the help of heuristic evolving latent projection (HELP) method, score plots of principal component analysis (PCA) successfully presented the grown footprints of tangerine peels. It implied that July might be the best harvest time for PCRV, November and December were better for PCR. Furthermore, hesperidin, nobiletin and tangeretin were screened as chemical markers by loadings of PCA. The HPLC-HELP-PCA strategy has shown its potential in optimization of harvest time and chemical markers' screening, which will have wide perspective in the analysis of "coupled TCMs".
Yi
Lun-zhao
LZ
Research Center of Modernization of Chinese Medicines, Central South University, Changsha 410083, China. yilunzhao@mail.csu.edu.cn
Yuan
Da-lin
DL
Liang
Yi-zeng
YZ
Xie
Pei-shan
PS
Zhao
Yu
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry* / Q000254:growth & development; D047309:Flavones / Q000032:analysis; D006569:Hesperidin / Q000032:analysis; D008516:Medicine, Chinese Traditional; D055432:Metabolomics; D025341:Principal Component Analysis; D012621:Seasons
D047309:Flavones; C008661:nobiletin; D006569:Hesperidin; C059006:tangeretin