Fluorescence properties of trans-ethyl-p-(dimethylamino) cinnamate in presence of bile acid host.
The knowledge of the formation of bile acid micellar aggregates is of great importance because of the biological significance of these compounds and their pharmacological applications. The intramolecular charge transfer (ICT) fluorescence property of trans-ethyl-p-(dimethylamino) cinnamate is used to study the micelles formed by aggregation of three most important bile acids, viz. cholic acid, deoxycholic acid and chenodeoxycholic acid by steady state and picosecond time-resolved fluorescence spectroscopy. The ICT fluorescence band intensity was found to increase with concomitant blue shift with the addition of bile acids. The blue shift in ICT fluorescence maxima as well as decrease in nonradiative decay constants in presence of bile acids indicate the passage of the probe towards the micro domains formed from the aggregated bile acids. Binding constant of the probe with micelles as well as critical micelle concentration and average polarity parameter of the micellar environments were obtained from the variation of fluorescence intensity on increasing concentration of bile acids in the medium.
Singh
T S
TS
Department of Chemistry, North-Eastern Hill University, Shillong 793 022, India.
Mitra
Sivaprasad
S
D001647:Bile Acids and Salts / Q000737:chemistry*; D002234:Carbodiimides / Q000737:chemistry; D002635:Chenodeoxycholic Acid / Q000737:chemistry; D019826:Cholic Acid / Q000737:chemistry; D002934:Cinnamates / Q000737:chemistry*; D003840:Deoxycholic Acid / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008823:Micelles; D013050:Spectrometry, Fluorescence
D001647:Bile Acids and Salts; D002234:Carbodiimides; D002934:Cinnamates; D005456:Fluorescent Dyes; D008823:Micelles; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C074139:1-ethyl-3-(3-dimethylaminoethyl)carbodiimide; D019826:Cholic Acid