title: The fungal glutathione S-transferase system. Evidence of new classes in the wood-degrading basidiomycete Phanerochaete chrysosporium.
abstract: The recent release of several basidiomycete genome sequences allows an improvement of the classification of fungal glutathione S-transferases (GSTs). GSTs are well-known detoxification enzymes which can catalyze the conjugation of glutathione to non-polar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. Following this mechanism, they are able to metabolize drugs, pesticides, and many other xenobiotics and peroxides. A genomic and phylogenetic analysis of GST classes in various sequenced fungi--zygomycetes, ascomycetes, and basidiomycetes--revealed some particularities in GST distribution, in comparison with previous analyses with ascomycetes only. By focusing essentially on the wood-degrading basidiomycete Phanerochaete chrysosporium, this analysis highlighted a new fungal GST class named GTE, which is related to bacterial etherases, and two new subclasses of the omega class GSTs. Moreover, our phylogenetic analysis suggests a relationship between the saprophytic behavior of some fungi and the number and distribution of some GST isoforms within specific classes.
authors: IFR 110 Ecosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation, Unité Mixte de Recherches INRA UHP 1136 Interaction Arbres Microorganismes, Université Nancy I BP 239, 54506 Vandoeuvre-lès-Nancy Cedex, France. mmorel@scbiol.uhp-nancy.fr
authors: Mélanie
authors: M
authors: Morel
authors: Andrew A
authors: AA
authors: Ngadin
authors: Michel
authors: M
authors: Droux
authors: Jean-Pierre
authors: JP
authors: Jacquot
authors: Eric
authors: E
authors: Gelhaye
mesh_terms: D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000201:enzymology*; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014934:Wood / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D020033:Protein Isoforms; D005982:Glutathione Transferase