title: Natural competence in strains of Actinobacillus pleuropneumoniae.
abstract: We have identified a highly transformable strain of Actinobacillus pleuropneumoniae whose competence is regulated by the competence-activator Sxy as in other Pasteurellaceae. Other strains were poorly transformable or nontransformable. The genomes of two poorly transformable strains contain intact sets of competence genes. Moreover, we show that the low competence of one of these strains is not due to an inability to induce sxy expression or to a defect in Sxy function, suggesting that some other component of the competence system is defective. Although the A. pleuropneumoniae sxy gene has only 24% identity to its Haemophilus influenzae homologue, both genes fully complemented an H. influenzae sxy knockout, demonstrating that Sxy function is conserved throughout the Pasteurellaceae.
authors: Molecular Infectious Disease Group, Department of Paediatrics, Imperial College London, London, UK. j.bosse@imperial.ac.uk
authors: Janine T
authors: JT
authors: Bossé
authors: Sunita
authors: S
authors: Sinha
authors: Timo
authors: T
authors: Schippers
authors: J Simon
authors: JS
authors: Kroll
authors: Rosemary J
authors: RJ
authors: Redfield
authors: Paul R
authors: PR
authors: Langford
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000235:genetics*; D001426:Bacterial Proteins / Q000502:physiology; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006193:Haemophilus influenzae / Q000235:genetics; D015534:Trans-Activators / Q000502:physiology; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators