Prophylactic role of taurine on arsenic mediated oxidative renal dysfunction via MAPKs/ NF-kappaB and mitochondria dependent pathways.
The present study has been designed and carried out to investigate the protective role of taurine (2-aminoethanesulphonic acid) against NaAsO(2) induced nephrotoxicity. Oral administration of arsenic increased the productions of ROS and RNS, enhanced lipid peroxidation, protein carbonylation and decreased intracellular antioxidant defence in the kidney tissue. Investigating the responsible signalling cascades, it was found that NaAsO(2) administration activates mitogen-activated protein kinases (MAPKs) and NF-kappaB in oxidative stress mediated renal dysfunction and induced apoptotic cell death by the reciprocal regulation of Bcl-2/Bad in association with reducing mitochondrial membrane potential and increased cytosolic cytochrome C as well. Treatment with taurine prior to arsenic administration effectively ameliorated As-induced oxidative renal dysfunctions and apoptotic cell death. Histological studies also support the experimental findings. Combining, results suggest that taurine possesses the ability to ameliorate arsenic-induced oxidative insult and renal damage, probably due to its antioxidant activity and functioning via MAPKs/NF-kappaB and mitochondria dependent pathways.
Roy
Anandita
A
Division of Molecular Medicine, Bose Institute, Kolkata, India.
Manna
Prasenjit
P
Sil
Parames C
PC
D000818:Animals; D018053:Arsenites / Q000037:antagonists & inhibitors* / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D017670:Sodium Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity; D013054:Spectrophotometry, Atomic; D013654:Taurine / Q000494:pharmacology*
D018053:Arsenites; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D017670:Sodium Compounds; D013654:Taurine; C017947:sodium arsenite; D020928:Mitogen-Activated Protein Kinases