title: Raphanus sativus extract protects against Zearalenone induced reproductive toxicity, oxidative stress and mutagenic alterations in male Balb/c mice.
abstract: Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. It has been implicated in several mycotoxicosis in farm animals and in humans. There is unequivocal evidence of reproductive toxicity of ZEN in male mice although the mechanism of action is unknown. Several reports suggest that exposure to ZEN resulted in oxidative stress, genotoxicity and perturbation of reproductive parameters. Therefore, the aim of the current study was to evaluate the protective effects of aqueous extract of Raphanus sativus growing in Tunisia against ZEN-induced reproductive toxicity and oxidative stress. Fifty male Balb/c mice were divided into five groups and treated for 28 days as follows: the control group, olive oil-treated groups, another treated with ZEN (40 mg/kg b.w), the last one treated with R. sativus extract alone (15 mg/kg b.w) and the other with ZEN + R. sativus extract. Testis samples were collected for the epididymal sperm count, testosterone concentration, and MDA level, GPx, CAT and SOD activities. Blood samples were collected for different biochemical analyses. Also, RAPD-PCR method was performed to assess the antigenotoxic effect of the extract in germ cells. The results indicated that ZEN-induced toxicological effects in accordance to those reported in the literature: decreasing in the sperm number, testosterone level and antioxidant enzyme status. The RAPD-PCR analysis revealed an alteration in the DNA bands patterns between control and ZEN-treated mice. The extract alone, rich in many antioxidant compounds, was safe and succeeded in counteracting the oxidative stress and protect against the toxicity resulting from ZEN.
authors: Laboratory of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences Bizerte, 7021 Zarzouna, Tunisia. jalila.bensalah@yahoo.fr
authors: Jalila
authors: J
authors: Ben Salah-Abbès
authors: Samir
authors: S
authors: Abbès
authors: Mosaad A
authors: MA
authors: Abdel-Wahhab
authors: Ridha
authors: R
authors: Oueslati
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D009929:Organ Size; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D020011:Protective Agents / Q000494:pharmacology*; D031224:Raphanus / Q000737:chemistry*; D012098:Reproduction / Q000187:drug effects*; D013076:Sperm Count; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D013739:Testosterone; D008315:Malondialdehyde; D015025:Zearalenone; D002374:Catalase; D013482:Superoxide Dismutase