VP7 and VP4 sequence analyses of rotavirus strains from Italian children with viraemia and acute diarrhoea.
Information on genotypes or variants associated with systemic rotavirus infection is lacking. Seven viraemic children with acute rotavirus diarrhoea were evaluated. All faecal strains were genotyped (5 G1P[8] type, 1 G9P[8], 1 G1-G4P[8]). Only 3 of 7 blood strains were typeable (G1P[8], G4P8, and G1P[ND]). A discrepancy between faecal and blood VP7 amino acid sequences (Ser-->Asp at position 94) was found in the first child. Two amino acid substitutions (Ile-->Val and Asn-->Ser at position 106 and 113) in the VP4 sequences were demonstrated in the second child. Complete correspondence was found in the third child. All of the observed amino acid substitutions may be involved in rotavirus neutralisation. We speculate that during co-infection by multiple strains, a preferential extraintestinal dissemination of some variants may occur, possibly because of differential viral characteristics.
Chiappini
Elena
E
Department of Paediatrics, University of Florence, Florence, Italy.
Arista
Serenella
S
Moriondo
Maria
M
De Grazia
Simona
S
Giammanco
Giovanni Maurizio
GM
Azzari
Chiara
C
Galli
Luisa
L
de Martino
Maurizio
M
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000596:Amino Acids / Q000235:genetics*; D000956:Antigens, Viral / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D003967:Diarrhea / Q000209:etiology* / Q000821:virology; D005243:Feces; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007558:Italy; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000097:blood / Q000150:complications / Q000821:virology; D014766:Viremia / Q000097:blood / Q000209:etiology* / Q000821:virology
D000596:Amino Acids; D000956:Antigens, Viral; D036022:Capsid Proteins