[Effects of cryopreservation on expression of connective tissue growth factor in rat kidney].
OBJECTIVE
To investigate the effect of cryopreservation (CP) on the expression of connective tissue growth factor (CTGF) in the renal tubular epithelial cells.


METHODS
A total of 40 male Wistar rats (weighing 230-250 g) were used in this study. En bloc removal with in situ cooling both kidneys and hypertonic citrate adenine preservation solution were adopted. The rat kidney was be preserved 0, 12, 24, 36 and 48 hours at 0-4 degrees C (n=8), respectively. The expression of CTGF of renal tubular epithelial cells was detected by using immunohistochemistry and in situ hybridization analysis.


RESULTS
The expression of CTGF was less in CP 0 hour group and CP 12 hours group, the positive unit (PU) values of CTGF protein were 5.91 +/- 2.30 and 5.57 +/- 2.40 (P > 0.05), respectively, and the PU values of CTGF mRNA were 6.24 +/- 2.79 and 6.51 +/- 2.43 (P > 0.05), respectively. The PU values of CTGF protein increased at CP 24 hours group (10.25 +/- 2.92), CP 36 hours group (14.31 +/- 2.83) and CP 48 hours group (18.11 +/- 3.94, P < 0.05), respectively, and the PU values of CTGF mRNA increased at CP 24 hours group (15.24 +/- 3.95), CP 36 hours group (19.20 +/- 4.73) and CP 48 hours group (23.09 +/- 4.40, P < 0.05), respectively; showing significant differences when compared with CP 0 hour group and CP 12 hours group (P < 0.05).


CONCLUSIONS
CTGF expression may increase with severe cold ischemia injury, and might play an important role in regeneration and repair of renal tubular epithelial cell injury.
He
Wenfang
W
Morphological Experiments Center of Medical College, Jinan University, Guangzhou Guangdong, 510630, P.R. China. thwf@jnu.edu.cn
Ding
Hongwen
H
Su
Zexuan
Z
D000818:Animals; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis*; D015925:Cryopreservation; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
D055513:Connective Tissue Growth Factor