title: [Advanced glycation end products induce expression of PAI-1 in cultured human proximal tubular epithelial cells through NADPH oxidase dependent pathway].
abstract: OBJECTIVE
  To investigate the effects of advanced glycation end products (AGES) on secretion of plasminogen activator inhibitor-1(PAI-1)by human proximal tubular epithelial cells and its NADPH oxidase dependent pathway.
  
  
  METHODS
  Human proximal tubular epithelial cells were cultured in vitro with indicated concentration of AGES modified human serum albumin (AGES-HSA). NADPH oxidase activity were detected by lucigenin-enhanced chemiluminescence. The production of PAI-1 was evaluated by enzyme-linked immunoadsorbent assay (ELISA). The PAI-1 mRNA expression was assayed by reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  AGES-HSA were associated with enhanced oxidative stress and NADPH oxidase activity. AGES-HSA upregulated the expression of PAI-1 mRNA and protein with dose and time dependent fashion. AGES-HSA-induced PAI-1 expression were significantly suppressed by the NAD(P)H oxidase inhibitors DPI, apocynin or O2- scavenger SOD.
  
  
  CONCLUSIONS
  AGES-HSA stimulate tubular epithelial cells to produce PAI-1 through activation of NADPH oxidase.
authors: Department of Nephrology, Nanfang Hospital, Southern Medical Unversity, Guangzhou 510515, China. fblfbl1982@163.com
authors: Bi-ling
authors: BL
authors: Fu
authors: Zhi-jian
authors: ZJ
authors: Guo
authors: Jian-wei
authors: JW
authors: Tian
authors: Zhi-qiang
authors: ZQ
authors: Liu
authors: Wei
authors: W
authors: Cao
mesh_terms: D002460:Cell Line; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D017127:Glycation End Products, Advanced; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics* / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D017127:Glycation End Products, Advanced; D017395:Plasminogen Activator Inhibitor 1; D012709:Serum Albumin; D019255:NADPH Oxidases