Electrospun gelatin/polyurethane blended nanofibers for wound healing.
In this study, we prepared a blended nanofiber scaffold using synthetic and natural polymers, polyurethane (PU) and gelatin respectively, using the electrospinning method to prepare a material for wound dressing. In order to confirm the properties of this gelatin/PU blended nanofiber scaffold, we performed scanning electron microscopy, atomic force microscopy, attenuated total reflectance Fourier-transform infrared spectroscopy, thermal gravimetric analysis, contact angle, water uptake, mechanical property, recovery, and degradation tests, and cellular response. The results obtained indicate that the mean diameter of these nanofibers was uniformly electrospun and ranged from 0.4 to 2.1 microm. According to the results, when the amount of gelatin in the blended solution decreased, the contact angle increased and water uptake of the scaffold decreased concurrently. In the mechanical tests, the blended nanofibrous scaffolds were elastic, and elasticity increased as the total amount of PU increased. Moreover, as the total amount of gelatin increased, the cell proliferation increased with the same amount of culture time. Therefore, this gelatin/PU blended nanofiber scaffold has potential application for use as a wound dressing.
Kim
Sung Eun
SE
Department of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Korea.
Heo
Dong Nyoung
DN
Lee
Jung Bok
JB
Kim
Jong Ryul
JR
Park
Sang Hyuk
SH
Jeon
Seong Ho
SH
Kwon
Il Keun
IK
D000818:Animals; D049109:Cell Proliferation; D005780:Gelatin / Q000032:analysis / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing / Q000502:physiology*
D011108:Polymers; D011140:Polyurethanes; D005780:Gelatin