Amycolatopsis tucumanensis sp. nov., a copper-resistant actinobacterium isolated from polluted sediments.
A novel actinomycete strain, ABO(T), isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABO(T) developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern (meso-diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family Pseudonocardiaceae and that it forms a monophyletic clade with Amycolatopsis eurytherma NT202(T). The DNA-DNA relatedness of strain ABO(T) to A. eurytherma DSM 44348(T) was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABO(T) represents a novel species in the genus Amycolatopsis, for which the name proposed is Amycolatopsis tucumanensis sp. nov. The type strain is ABO(T) (=DSM 45259(T) =LMG 24814(T)).
Albarracín
Virginia Helena
VH
Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina.|Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CONICET, Av. Belgrano y Pasaje Caseros, 4000 Tucumán, Argentina.
Alonso-Vega
Pablo
P
Departamento de Microbiología y Genética, Edificio Departamental, Lab. 205, Campus Miguel de Unamuno, Universidad de Salamanca, Salamanca, Spain.
Trujillo
Martha E
ME
Departamento de Microbiología y Genética, Edificio Departamental, Lab. 205, Campus Miguel de Unamuno, Universidad de Salamanca, Salamanca, Spain.
Amoroso
María Julia
MJ
Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina.|Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CONICET, Av. Belgrano y Pasaje Caseros, 4000 Tucumán, Argentina.
Abate
Carlos Mauricio
CM
Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina.|Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CONICET, Av. Belgrano y Pasaje Caseros, 4000 Tucumán, Argentina.|Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina.
D000192:Actinomycetales / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D003300:Copper / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology; D014877:Water Pollution, Chemical
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D003300:Copper