title: Involvement of E-cadherin cleavage in reperfusion injury.
abstract: OBJECTIVE
  E-cadherin is a major cell-to-cell adhesion molecule, of which the ectodomain is cleaved from epithelial cells to yield a soluble form after the pathological alteration of the alveolar epithelium. We investigated the excretion level of soluble E-cadherin in a rat lung isotransplant model, and demonstrated the involvement of this molecule in the pathogenesis of reperfusion injury after lung transplantation.
  
  
  METHODS
  Inbred male Lewis rats were used as both donor and recipient animals, and they were subjected to left lung isotransplantation. After 6 h of ischaemia, the left lung was transplanted into a recipient rat and reperfused for 4 h. The animals were injected intravenously with (125)I-labelled albumin at 3 h after the onset of reperfusion as a marker of pulmonary albumin leakage. We assessed pulmonary alveolar septal damage quantitatively based on the (125)I-albumin concentration ratio of bronchoalveolar lavage fluid (BALF) to plasma. Soluble E-cadherin fragments were detected in BALF on Western blot analysis using affinity-purified antibodies specific to rat E-cadherin synthetic peptides.
  
  
  RESULTS
  The BALF supernatant-to-plasma ratio of the graft lung was significantly increased compared to that of the control group. Western blot analysis showed a marked release of soluble E-cadherin into BALF, and its increase in BALF was associated with alveolar septal damage.
  
  
  CONCLUSIONS
  These results suggest that one potential mechanism of lung reperfusion injury involves the cleavage of E-cadherin.
authors: Department of General Thoracic Surgery, National Hospital Organization Tokyo Medical Center, Tokyo, Japan. tgoto@ntmc.hosp.go.jp
authors: Taichiro
authors: T
authors: Goto
authors: Akitoshi
authors: A
authors: Ishizaka
authors: Masahiko
authors: M
authors: Katayama
authors: Mitsutomo
authors: M
authors: Kohno
authors: Sadatomo
authors: S
authors: Tasaka
authors: Seitaro
authors: S
authors: Fujishima
authors: Koichi
authors: K
authors: Kobayashi
authors: Hiroaki
authors: H
authors: Nomori
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D015820:Cadherins / Q000378:metabolism*; D007457:Iodine Radioisotopes; D016040:Lung Transplantation / Q000473:pathology; D008297:Male; D011654:Pulmonary Edema / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012709:Serum Albumin
chemical_list: D015820:Cadherins; D007457:Iodine Radioisotopes; D012709:Serum Albumin