title: High consistency enzymatic hydrolysis of hardwood substrates.
abstract: The feasibility of using a laboratory peg mixer to carry out high consistency enzymatic hydrolysis of lignocellulosic substrates was investigated. Two hardwood substrates, unbleached hardwood pulp (UBHW) and organosolv pretreated poplar (OPP), were used in this study. Hydrolysis of UBHW and OPP at 20% substrate consistency led to a high glucose concentration in the final hydrolysate. For example, a 48 h enzymatic hydrolysis of OPP resulted in a hydrolysate with 158 g/L of glucose. This is the highest glucose concentration ever obtained from enzymatic hydrolysis of lignocellulosic substrates. Fermentation of UBHW and OPP hydrolysates with high glucose content led to high ethanol concentrations, 50.4 and 63.1 g/L, respectively after fermentation. Our results demonstrate that using common pulping equipment to carry out high consistency hydrolysis can overcome the rheological problems and greatly increase the sugar and ethanol concentrations after the hydrolysis and fermentation.
authors: FPInnovations Paprican Division (PAPRICAN), 570 boul. St-Jean, Pointe-Claire, Quebec, Canada H9R 3J9. xiao.zhang@fpinnovations.ca
authors: Xiao
authors: X
authors: Zhang
authors: Wenjuan
authors: W
authors: Qin
authors: Michael G
authors: MG
authors: Paice
authors: John N
authors: JN
authors: Saddler
mesh_terms: D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D004867:Equipment Design; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D032107:Populus; D012212:Rheology; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013997:Time Factors
chemical_list: D002241:Carbohydrates; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D005947:Glucose