title: Submucosal tunneling using endoscopic submucosal dissection for peritoneal access and closure in natural orifice transluminal endoscopic surgery: a porcine survival study.
abstract: OBJECTIVE
  Safe peritoneal access and gastric closure are the most important concerns in the clinical application of natural orifice transluminal endoscopic surgery (NOTES). We aimed to clarify the feasibility of a submucosal tunnel technique using endoscopic submucosal dissection (ESD) for transgastric peritoneal access and subsequent closure for NOTES.
  
  
  METHODS
  Seven female pigs, each weighing about 40 kg were included in the study. The following procedures were performed: (i) after injection of normal saline into the submucosa, the mucosa was cut with a flex knife; (ii) the submucosal layer was dissected using an insulation-tipped electrosurgical knife to make a narrow longitudinal 50-mm submucosal tunnel; (iii) a small incision was made at the end of the tunnel and enlarged with a dilation balloon. After transgastric peritoneoscopy, the mucosal incision site was closed with clips. The following outcome measures were used: (a) evaluation of the technical feasibility of making a submucosal tunnel; (b) clinical monitoring for 7 days; (c) follow-up endoscopy and necropsy; and (d) peritoneal fluid culture.
  
  
  RESULTS
  Natural orifice transluminal endoscopic peritoneoscopy with a submucosal tunnel was successfully carried out in all pigs. The pigs recovered well, without signs of peritonitis. Follow-up endoscopy showed healing of mucosal incision sites without open defects. Necropsy revealed no findings of peritonitis, confirming completeness of gastric closure; there was a thin scar in one pig and adhesion of the omentum in six pigs. Peritoneal fluid culture demonstrated no bacterial growth.
  
  
  CONCLUSIONS
  The submucosal tunnel technique is feasible and effective for transgastric peritoneal access and closure.
authors: Department of Gastroenterological Surgery, Oita University Faculty of Medicine, Yufu, Oita, Japan. Fumitaka@med.oita-u.ac.jp
authors: F
authors: F
authors: Yoshizumi
authors: K
authors: K
authors: Yasuda
authors: K
authors: K
authors: Kawaguchi
authors: K
authors: K
authors: Suzuki
authors: N
authors: N
authors: Shiraishi
authors: S
authors: S
authors: Kitano
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004210:Dissection; D005240:Feasibility Studies; D005260:Female; D005753:Gastric Mucosa / Q000601:surgery*; D005773:Gastroscopy / Q000379:methods*; D010535:Laparoscopy / Q000379:methods*; D034421:Sus scrofa; D016896:Treatment Outcome