Candida golubevii sp. nov., an asexual yeast related to Metschnikowia lunata.
Two strains of a novel yeast species were isolated from insect frass and a flower in Thailand and Brazil, respectively. The strain from Thailand was isolated from insect frass collected in Than-Tong waterfall, Nong Khai Province, whereas the strain from Brazil was recovered from a flower of Ipomoea sp. collected on the banks of the Paraguai River in the state of Mato Grosso do Sul. The sequences of the D1/D2 domains of the large subunit of the rDNA of both strains were identical. This novel species belonged to the Metschnikowia clade and was related to Metschnikowia lunata. No signs of sporulation were observed for the two strains on various culture media. The novel species, Candida golubevii sp. nov., is proposed to accommodate these isolates. The type strain is BCC 8332(T) (=CBS 11362(T)=NBRC 105679(T)).
Rosa
Carlos A
CA
Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
Jindamorakot
Sasitorn
S
BIOTEC Culture Collection, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
Limtong
Savitree
S
Department of Microbiology, Faculty of Science, Kasetsart University, 50 Paholyothin Rd., Bangkok 10900, Thailand.
Nakase
Takashi
T
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisaraza, Chiba 292-0818, Japan.
Pagnocca
Fernando C
FC
Departamento de Bioquímica e Microbiologia, Centro de Estudos de Insetos Sociais, Universidade Estadual Paulista, Avenida 24 A, 1515, Rio Claro, SP 13506-900, Brazil.
Lachance
Marc-André
MA
Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada.
D000818:Animals; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D035264:Flowers / Q000382:microbiology; D007313:Insecta / Q000382:microbiology*; D027741:Ipomoea / Q000382:microbiology*; D055163:Metschnikowia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012100:Reproduction, Asexual
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S