Marichromatium fluminis sp. nov., a slightly alkaliphilic, phototrophic gammaproteobacterium isolated from river sediment.
An anoxygenic, phototrophic gammaproteobacterium designated strain JA418(T) was isolated from a sediment sample collected from the Baitarani River, Orissa, India. The bacterium was a Gram-negative, motile rod with a single polar flagellum. Bacteriochlorophyll a and rhodopin were the major photosynthetic pigments. The organism grew best at slightly alkaline pH (8-8.5) and lacked chemotrophic growth. The major fatty acids were C(16 : 0), C(16 : 1)omega7c/C(16 : 1)omega6c and C(18 : 1)omega7c. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain JA418(T) clusters with species of the genus Marichromatium belonging to the class Gammaproteobacteria. The highest 16S rRNA gene sequence similarities of strain JA418(T) were found with the type strains of Marichromatium gracile (95.9 %), Marichromatium indicum (95.6 %), Marichromatium purpuratum (95.5 %) and Marichromatium bheemlicum (95.6 %). The DNA base composition of strain JA418(T) was 71.4 mol% G+C (by HPLC). Based on the 16S rRNA gene sequence analysis and physiological and chemotaxonomic characteristics, strain JA418(T) is sufficiently different from other Marichromatium species to merit the description of a novel species, Marichromatium fluminis sp. nov., to accommodate it. The type strain is JA418(T) (=KCTC 5717(T) =NBRC 105221(T)).
Sucharita
K
K
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
Shiva Kumar
E
E
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
Sasikala
Ch
C
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
Panda
B B
BB
Department of Botany, Bharampur University, Bharampur, Orissa, India.
Takaichi
S
S
Department of Biology, Nippon Medical School, Kosugi-cho, Nakahara, Kawasaki 211-0063, Japan.
Ramana
Ch V
CV
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002841:Chromatiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007194:India; D008969:Molecular Sequence Data; D010641:Phenotype; D052817:Phototrophic Processes; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S