Transient anomalous diffusion of telomeres in the nucleus of mammalian cells.
We measured individual trajectories of fluorescently labeled telomeres in the nucleus of eukaryotic cells in the time range of 10(-2)-10(4)sec by combining a few acquisition methods. At short times the motion is subdiffusive with r2 approximately talpha and it changes to normal diffusion at longer times. The short times diffusion may be explained by the reptation model and the transient diffusion is consistent with a model of telomeres that are subject to a local binding mechanism with a wide but finite distribution of waiting times. These findings have important biological implications with respect to the genome organization in the nucleus.
Bronstein
I
I
Physics Department & Institute for Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel.
Israel
Y
Y
Kepten
E
E
Mai
S
S
Shav-Tal
Y
Y
Barkai
E
E
Garini
Y
Y
D001859:Bone Neoplasms; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry* / Q000235:genetics; D004058:Diffusion; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D008956:Models, Chemical; D012516:Osteosarcoma; D013194:Staining and Labeling / Q000379:methods; D016615:Telomere / Q000737:chemistry* / Q000235:genetics
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI