title: Phage display selection, analysis, and prediction of B cell epitopes.
abstract: Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided.
authors: Tel Aviv University, Tel Aviv, Israel.
authors: Natalia Tarnovitski
authors: NT
authors: Freund
authors: Harvard Medical School, Charlestown, Massachusetts.
authors: David
authors: D
authors: Enshell-Seijffers
authors: Tel Aviv University, Tel Aviv, Israel.
authors: Jonathan M
authors: JM
authors: Gershoni
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D001671:Biochemistry; D032701:Biomimetics; D019295:Computational Biology; D003198:Computer Simulation; D018604:Epitope Mapping / Q000379:methods*; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005818:Genetic Engineering; D006801:Humans; D017912:Inovirus; D018716:Molecular Mimicry; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D019151:Peptide Library
chemical_list: D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D010446:Peptide Fragments; D019151:Peptide Library