title: Insertion shuttle with carboxyl terminated self-assembled monolayer coatings for implanting flexible polymer neural probes in the brain.
abstract: Penetrating microscale microelectrodes made from flexible polymers tend to bend or deflect and may fail to reach their target location. The development of flexible neural probes requires methods for reliable and controlled insertion into the brain. Previous approaches for implanting flexible probes into the cortex required modifications that negate the flexibility, limit the functionality, or restrict the design of the probe. This study investigated the use of an electronegative self-assembled monolayer (SAM) as a coating on a stiff insertion shuttle to carry a polymer probe into the cerebral cortex, and then the detachment of the shuttle from the probe by altering the shuttle's hydrophobicity. Polydimethylsiloxane (PDMS) and polyimide probes were inserted into an agarose in vitro brain model using silicon insertion shuttles. The silicon shuttles were coated with a carboxyl terminal SAM. The precision of insertion using the shuttle was measured by the percentage displacement of the probe upon shuttle removal after the probe was fully inserted. The average relative displacement of polyimide probes inserted with SAM-coated shuttles was (1.0+/-0.66)% of the total insertion depth compared to (26.5+/-3.7)% for uncoated silicon shuttles. The average relative displacement of PDMS probes was (2.1+/-1.1)% of the insertion depth compared to 100% (complete removal) for uncoated silicon shuttles. SAM-coated shuttles were further validated through their use to reliably insert PDMS probes in the cerebral cortex of rodents. This study found that SAM-coated silicon shuttles are a viable method for accurately and precisely inserting flexible neural probes in the brain.
authors: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. tkozai@umich.edu
authors: Takashi D Yoshida
authors: TD
authors: Kozai
authors: Daryl R
authors: DR
authors: Kipke
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery*; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005227:Fatty Acids / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008422:Materials Testing; D008839:Microelectrodes; D044085:Microfluidics; D008953:Models, Anatomic; D009757:Nylons / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012117:Resins, Synthetic / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013238:Stereotaxic Techniques / Q000295:instrumentation*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
chemical_list: C505222:11-mercaptoundecanoic acid; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D005227:Fatty Acids; D009757:Nylons; D011108:Polymers; D012117:Resins, Synthetic; D013438:Sulfhydryl Compounds; C085707:poly(dimethylsiloxane)-polyamide copolymer; C029945:polyimide resin; D012685:Sepharose; D012825:Silicon