title: Goat whey fermentation by Kluyveromyces marxianus and Lactobacillus rhamnosus release tryptophan and tryptophan-lactokinin from a cryptic zone of alpha-lactalbumin.
abstract: Angiotensin-I-Converting Enzyme (ACE) inhibitors peptides were produced from unsupplemented acid goat whey fermented aerobically for 168 h by Kluyveromyces marxianus and Lactobacillus rhamnosus. This yeast-lactobacillus association is GRAS. Two novel lactokinins were identified: NYW and W with IC50 of 20 and 0.86 mum respectively. They both were resistant toward simulated gastrointestinal digestion. In addition, WLAHK was found in the hydrolysate. These three sequences belong to f(99-110) of alpha-la which seems to be a lactokinin cryptic zone. W was the major molecule released by the fermentation process. Considering that W is the precursor of serotonin, the hydrolysate produced could be of interest for the generation of functional health ingredient involved in regulation of affective disorders and hypertension.
authors: Laboratoire LIENSs, CNRS UMR 6250, site Marie Curie, UFR Sciences Technologies Santé, Avenue Michel Crépeau, F-17042 La Rochelle cedex 01, France. vhamme01@univ-lr.fr
authors: Vanessa
authors: V
authors: Hamme
authors: Frederic
authors: F
authors: Sannier
authors: Jean-Marie
authors: JM
authors: Piot
authors: Stephanie
authors: S
authors: Bordenave-Juchereau
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D005260:Female; D005285:Fermentation; D006041:Goats; D007716:Kluyveromyces / Q000378:metabolism*; D007768:Lactalbumin / Q000737:chemistry / Q000378:metabolism; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D008892:Milk / Q000378:metabolism / Q000382:microbiology*; D008894:Milk Proteins / Q000737:chemistry / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism*; D000067816:Whey Proteins
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D008894:Milk Proteins; D000067816:Whey Proteins; C121294:lactokinins; D014364:Tryptophan; D007768:Lactalbumin