Assembly of Coenzyme Q10 nanostructure resembling nascent discoidal high density lipoprotein particle.
There are tremendous drug candidates that suffer from insolubility in water. In the present study, it is shown that Coenzyme Q10 (CoQ10), a model water-insoluble compound, can be nanoparticulated into a water-soluble form using apolipoprotein A-I (apoA-I). Similar to the way that apoA-I forms nascent discoidal high density lipoprotein (ndHDL) particles by bordering acyl chain tails of phospholipids, CoQ10 could be enclosed into the circle of a disk made of apoA-Is. The resulting nanostructure of CoQ10 and apoA-I was water-soluble with a size of approximately 12 nm in diameter and was physically more robust than liposome. We expect that the strategy suggested in this study can be exploited to assemble nano-sized, water-soluble structures of various water-insoluble drug candidates.
Shin
Jae-Yoon
JY
School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Gyeongido 440-746, Republic of Korea.
Shin
Jae-Il
JI
Kim
Jun-Seob
JS
Yang
Yoo-Soo
YS
Hwang
Yunha
Y
Yang
Joo-Sung
JS
Shin
Dongwoo
D
Seo
Jin-Ho
JH
Jin
Yong-Su
YS
Park
Yong-Cheol
YC
Hwang
Jae-Sung
JS
Kweon
Dae-Hyuk
DH
D016632:Apolipoprotein A-I / Q000737:chemistry*; D008075:Lipoproteins, HDL / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry; D012995:Solubility; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D010713:Phosphatidylcholines; D014867:Water; D014451:Ubiquinone; C024989:coenzyme Q10; C028694:1-palmitoyl-2-oleoylphosphatidylcholine