title: Effect of platelet-activating factor on motility, plasmalemma integrity, the process of capacitation and acrosome reaction of fresh and cryopreserved boar spermatozoa.
abstract: The aim of the present study was to establish the effects of platelet-activating factor (PAF) on selected movement parameters, plasmalemma integrity, capacitation process and acrosome reaction in cryopreserved boar spermatozoa. A positive effect of PAF addition to cryopreserved semen on sperm motility was demonstrated, particularly with the application of phospholipid concentration of 1 x 10(-6)M-1 x 10(-5)M. A moderate induction of plasmalemma damage of cryopreserved spermatozoa was observed when PAF was used at a low concentration (1 x 10(-8)M-1 x 10(-7)M). The rate at which PAF induced the process of capacitation was inversely proportional to its concentration in the sample (the highest for the concentration of 1 x 10(-8)M, and the lowest at 1 x 10(-5)M). In turn, the strongest induction of acrosome reaction of spermatozoa was observed in samples with the addition of PAF at a concentration of 1 x 10(-7)M. The results obtained suggest that the application of PAF supplement to post-thawed boar semen can be used as a laboratory test of the ability of spermatozoa to induce the acrosome reaction.
authors: Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn-Kortowo, Poland. wladyslaw.kordan@uwm.edu.pl
authors: W
authors: W
authors: Kordan
authors: M
authors: M
authors: Lecewicz
authors: J
authors: J
authors: Tobolski
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects*; D000818:Animals; D015925:Cryopreservation / Q000662:veterinary; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D012662:Semen Preservation / Q000662:veterinary; D013075:Sperm Capacitation / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013552:Swine / Q000502:physiology*
chemical_list: D010972:Platelet Activating Factor