title: Efficacy of an indirect immunofluorescence test in the diagnosis of canine leishmaniosis.
abstract: Of 146 dogs from a visceral leishmaniosis-endemic area that tested seronegative by indirect immunofluorescence (IIF) on blood samples collected on filter paper (IIFp), 51 (34.9%) and 10 (6.8%) tested positive by IIF on serum samples (IIFs) and enzyme immunoassay, respectively. Three samples (2.0%) tested positive by PCR. Leishmania chagasi was isolated from the skin of five (3.4%) dogs. Amastigote forms were identified in two of these five animals following histopathological and immunohistochemical examination. The findings highlight that detection methods such as IIFp can permit dogs infected with L. chagasi to remain undetected in endemic areas with attendant consequences for the epidemiology of infection both in the canine and human populations.
authors: Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. fabiano.figueiredo@ipec.fiocruz.br
authors: F B
authors: FB
authors: Figueiredo
authors: M F
authors: MF
authors: Madeira
authors: R C
authors: RC
authors: Menezes
authors: R S
authors: RS
authors: Pacheco
authors: M Q
authors: MQ
authors: Pires
authors: M C
authors: MC
authors: Furtado
authors: A G
authors: AG
authors: Pinto
authors: T M P
authors: TM
authors: Schubach
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D019353:Endemic Diseases / Q000517:prevention & control / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary*; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity