Estimation of the effective orientation of the SHG source in primary cortical neurons.
In this paper we provide, for the first time to our knowledge, the effective orientation of the SHG source in cultured cortical neuronal processes in vitro. This is done by the use of the polarization sensitive second harmonic generation (PSHG) imaging microscopy technique. By performing a pixel-level resolution analysis we found that the SHG dipole source has a distribution of angles centered at thetae =33.96 degrees , with a bandwidth of Deltathetae = 12.85 degrees . This orientation can be related with the molecular geometry of the tubulin heterodimmer contained in microtubules.
Psilodimitrakopoulos
Sotiris
S
ICFO-Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860 Castelldefels, Barcelona, Spain.
Petegnief
Valerie
V
Soria
Guadalupe
G
Amat-Roldan
Ivan
I
Artigas
David
D
Planas
Anna M
AM
Loza-Alvarez
Pablo
P
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology*; D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008962:Models, Theoretical; D009474:Neurons / Q000166:cytology*; D055096:Optical Devices; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation