Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl)aminomethane buffer between 390 and 880 nm.
The refractivity of wild-type bacteriorhodopsin (bR(WT)) suspended in tris(hydroxymethyl)aminomethane (TRIS) buffer has been measured in the spectral range of 390-840 nm by the method of angle of minimal deviation with the use of a hollow glass prism. The refractive indices of pure bR(WT) as well as of TRIS buffer have been determined from the concentration dependent refraction values. Sellmeier-type dispersion equations have been fitted for both the TRIS buffer and pure bR(WT).
Heiner
Zsuzsanna
Z
Department of Optics and Quantum Electronics, University of Szeged, P.O. Box 406, Szeged 6701, Hungary. zheiner@brc.hu
Osvay
Károly
K
D001436:Bacteriorhodopsins / Q000737:chemistry*; D002021:Buffers; D004305:Dose-Response Relationship, Drug; D005898:Glass; D006217:Halobacterium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015233:Models, Statistical; D055095:Optics and Photonics; D011312:Pressure; D012031:Refractometry; D015203:Reproducibility of Results; D013696:Temperature; D014325:Tromethamine / Q000737:chemistry*
D002021:Buffers; D014325:Tromethamine; D001436:Bacteriorhodopsins