Redox and ATP control of photosynthetic cyclic electron flow in Chlamydomonas reinhardtii (I) aerobic conditions.
Assimilation of atmospheric CO2 by photosynthetic organisms such as plants, cyanobacteria and green algae, requires the production of ATP and NADPH in a ratio of 3:2. The oxygenic photosynthetic chain can function following two different modes: the linear electron flow which produces reducing power and ATP, and the cyclic electron flow which only produces ATP. Some regulation between the linear and cyclic flows is required for adjusting the stoichiometric production of high-energy bonds and reducing power. Here we explore, in the green alga Chlamydomonas reinhardtii, the onset of the cyclic electron flow during a continuous illumination under aerobic conditions. In mutants devoid of Rubisco or ATPase, where the reducing power cannot be used for carbon fixation, we observed a stimulation of the cyclic electron flow. The present data show that the cyclic electron flow can operate under aerobic conditions and support a simple competition model where the excess reducing power is recycled to match the demand for ATP.
Alric
Jean
J
UMR 7141, CNRS et Université Pierre et Marie Curie (Paris VI), Institut de Biologie Physico-Chimique, 13 Rue Pierre et Marie Curie 75005 Paris, France. jean.alric@ibpc.fr
Lavergne
Jérôme
J
Rappaport
Fabrice
F
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000332:Aerobiosis; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000254:growth & development / Q000378:metabolism*; D000460:Chlorophyta / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport; D007700:Kinetics; D008027:Light; D010084:Oxidation-Reduction; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology*; D010944:Plants / Q000378:metabolism; D013053:Spectrophotometry
D002245:Carbon Dioxide; D000255:Adenosine Triphosphate; D004237:Diuron