title: Enzymatic activity versus structural dynamics: the case of acetylcholinesterase tetramer.
abstract: The function of many proteins, such as enzymes, is modulated by structural fluctuations. This is especially the case in gated diffusion-controlled reactions (where the rates of the initial diffusional encounter and of structural fluctuations determine the overall rate of the reaction) and in oligomeric proteins (where function often requires a coordinated movement of individual subunits). A classic example of a diffusion-controlled biological reaction catalyzed by an oligomeric enzyme is the hydrolysis of synaptic acetylcholine (ACh) by tetrameric acetylcholinesterase (AChEt). Despite decades of efforts, the extent to which enzymatic efficiency of AChEt (or any other enzyme) is modulated by flexibility is not fully determined. This article attempts to determine the correlation between the dynamics of AChEt and the rate of reaction between AChEt and ACh. We employed equilibrium and nonequilibrium electro-diffusion models to compute rate coefficients for an ensemble of structures generated by molecular dynamics simulation. We found that, for the static initial model, the average reaction rate per active site is approximately 22-30% slower in the tetramer than in the monomer. However, this effect of tetramerization is modulated by the intersubunit motions in the tetramer such that a complex interplay of steric and electrostatic effects either guides or blocks the substrate into or from each of the four active sites. As a result, the rate per active site calculated for some of the tetramer structures is only approximately 15% smaller than the rate in the monomer. We conclude that structural dynamics minimizes the adverse effect of tetramerization, allowing the enzyme to maintain similar enzymatic efficiency in different oligomerization states.
authors: Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, Houston, Texas, USA. alemayehu.g.abebe@uth.tmc.edu
authors: Alemayehu A
authors: AA
authors: Gorfe
authors: Benzhuo
authors: B
authors: Lu
authors: Zeyun
authors: Z
authors: Yu
authors: J Andrew
authors: JA
authors: McCammon
mesh_terms: D000109:Acetylcholine / Q000737:chemistry / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism*; D003198:Computer Simulation; D004058:Diffusion; D004548:Elasticity; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D008958:Models, Molecular; D016012:Poisson Distribution; D011487:Protein Conformation; D055503:Protein Multimerization; D055672:Static Electricity
chemical_list: D000110:Acetylcholinesterase; D000109:Acetylcholine