Protective effect of ursolic acid from Cornus officinalis on the hydrogen peroxide-induced damage of HEI-OC1 auditory cells.
The fruits of Cornus officinalis have been used in traditional oriental medicine for treatment of inner ear diseases, such as tinnitus and hearing loss. In the present study, we investigated the protective effect of C. officinalis on hydrogen peroxide-induced cytotoxicity in HEI-OC1 auditory cells. The results from bioassay-guided fractionation of methanol extract of C. officinalis fruits showed that ursolic acid is a major active component. Ursolic acid (0.05-2 microg/ml) had protective effect against the HEI-OC1 cell damage and reduced lipid peroxidation in a dose-dependent manner. In addition, pre-treatment with ursolic acid significantly attenuated the decrease of activities of catalase (CAT) and glutathione peroxidase (GPX), but superoxide dismutase (SOD) activity was not significantly affected by ursolic acid. These results indicate that ursolic acid protects hydrogen peroxide-induced HEI-OC1 cell damage through inhibition of lipid peroxidation and induction of antioxidant enzymes, CAT and GPX, and may be one of the active components responsible for these effects of C. officinalis fruits.
Yu
Hyeon-Hee
HH
Department of Food and Nutrition, Kunsan National University, Kunsan, South Korea.
Hur
Jong-Moon
JM
Seo
Se-Jeong
SJ
Moon
Hae-Dalma
HD
Kim
Hyun-Jin
HJ
Park
Rae-Kil
RK
You
Yong-Ouk
YO
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005591:Chemical Fractionation; D029768:Cornus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000378:metabolism; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D000097245:Ursolic Acid
D002374:Catalase; D005979:Glutathione Peroxidase; D006861:Hydrogen Peroxide; D000432:Methanol; D010936:Plant Extracts; D020011:Protective Agents; D013482:Superoxide Dismutase; D014315:Triterpenes; D000097245:Ursolic Acid