Urinary excretion of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in nonsmokers and in smokers of cigarettes with different ISO tar yields.
Polycyclic aromatic hydrocarbons (PAH) are products of the incomplete combustion of organic materials, and they occur ubiquitously in the environment. They are also present in tobacco smoke. Some PAH have been classified as carcinogens; therefore, it is important to develop and assess suitable biomarkers for PAH exposure. A high-performance liquid chromatographic method with fluorescence detection was developed to determine 1- and 2-hydroxynaphthalene (1- and 2-OH-Nap), 2-hydroxyfluorene (2-OH-Flu), 2-/3-hydroxyphenanthrene (2-/3-OH-Phe), 1-/9-hydroxyphenanthrene (1-/9-OH-Phe), and 1-hydroxypyrene (1-OH-Pyr) in human urine. The method is sensitive (LOQ ranging from 0.01 ng/mL for 1-OH-Pyr to 1 ng/mL for the naphthols), precise (interday precision ranging from 1.4 to 6.9%), and accurate (97-106%). The method was applied to 108 urine samples from 25 nonsmokers and 83 smokers. Smokers excreted significantly higher amounts of 1-OH-Nap (16.1 vs. 2.9 microg/24 h), 2-OH-Nap (20.9 vs. 9.7 microg/24 h), 2-OH-Flu (1.87 vs. 0.75 microg/24 h), 2-/3-OH-Phe (0.73 vs. 0.50 microg/24 h), 1-/9-OH-Phe (0.66 vs. 0.35 microg/24 h), and 1-OH-Pyr (0.36 vs. 0.20 microg/24 h) compared to nonsmokers. In conclusion, the method is suitable for discriminating PAH exposure between different ISO tar yield cigarette smokers, and it may be applicable in evaluating future potential reduced exposure tobacco products.
Hagedorn
Heinz-Werner
HW
Analytisch-Biologisches Forschungslabor GmbH, Goethestrasse 20, 80336 Munich, Germany.
Scherer
Gerhard
G
Engl
Johannes
J
Riedel
Kirsten
K
Cheung
Francis
F
Errington
Graham
G
Shepperd
Jim
J
McEwan
Mike
M
D015415:Biomarkers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry; D006801:Humans; D011084:Polycyclic Aromatic Hydrocarbons / Q000652:urine*; D015203:Reproducibility of Results; D012907:Smoking / Q000652:urine*; D013638:Tars / Q000032:analysis*
D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D013638:Tars