A thermosensitive chitosan-based hydrogel barrier for post-operative adhesions' prevention.
Post-operative peritoneal adhesions are common and serious complications for surgeons. They can cause pelvic pain, infertility, and potentially lethal bowel obstruction. We synthesized injectable hydrogels that formed by chemical modification through grafted hydrobutyl groups to chitosan chains. Gelation of hydroxybutyl chitosan (HBC) occurs in less than 60 s. Once formed, it can also be recovered completely. The residue time of hydrogels can extend to 4 weeks in Kunming mice. HBC hydrogels showed mild cytotoxicity to mice fibroblast cell (L929) and human vascular endothelial cell (ECV-304) in vitro and were biocompatible in the murine muscles, causing no adhesions for 4 weeks. HBC gels can form a durable barrier between defected cecum and abdominal wall. In a mice sidewall defect-bowel abrasion model, HBC gels showed significant efficacy in reducing adhesion formation.
Wei
Chang-Zheng
CZ
Department of Orthopedics in Changzheng Hospital of Second Military Medical University, Shanghai 200003, China.
Hou
Chun-Lin
CL
Gu
Qi-Sheng
QS
Jiang
Li-Xia
LX
Zhu
Bin
B
Sheng
Ai-Lian
AL
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D000267:Tissue Adhesions / Q000473:pathology* / Q000517:prevention & control*; D016896:Treatment Outcome
D001672:Biocompatible Materials; D020100:Hydrogels; D048271:Chitosan