title: Tag-probe labeling methods for live-cell imaging of membrane proteins.
abstract: Instead of using reconstituted proteoliposomes, in situ investigations of membrane proteins in living cell membranes are important because the heterogeneous and dynamic nature of biomembranes significantly affects their behavior. Protein-specific labeling is a key technique for the detection of a target protein by fluorescence measurements, particularly fluorescence microscopy. However, conventional genetic fusion with fluorescent proteins has several shortcomings. Post-translational labeling methods using a genetically encodable tag and synthetic probes targeting to the tag can overcome these limitations. This review summarizes emerging tag-probe techniques for labeling specific membrane proteins and their applications, including endocytotic internalization, partitioning to specific membrane domains, interprotein interactions, and conformational changes.
authors: Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
authors: Yoshiaki
authors: Y
authors: Yano
authors: Katsumi
authors: K
authors: Matsuzaki
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D008565:Membrane Proteins