title: Chryseoglobus frigidaquae gen. nov., sp. nov., a novel member of the family Microbacteriaceae.
abstract: A motile, rod-shaped, yellow-pigmented bacterium, designated strain CW1(T), was isolated from a water-cooling system in the Republic of Korea. Cells were Gram-stain-positive, aerobic, catalase-positive and oxidase-negative. Strain CW1(T) formed slender rods with unusual bulbous protuberances. The major fatty acids were iso-C(16 : 1) (33.7 %), anteiso-C(15 : 0) (27.2 %), iso-C(14 : 0) (13.3 %) and C(16 : 0) (10.8 %). The cell-wall peptidoglycan was of type B2beta, containing lysine as the diamino acid. The respiratory quinones were menaquinones with 12, 13 and 14 isoprene units. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW1(T) formed an evolutionary lineage within the radiation enclosing members of the family Microbacteriaceae and was related to, but distant from, members of the genera Microcella and Yonghaparkia. On the basis of the evidence presented, strain CW1(T) is considered to represent a novel species of a new genus in the family Microbacteriaceae, for which the name Chryseoglobus frigidaquae gen. nov., sp. nov. is proposed. The type strain of Chryseoglobus frigidaquae is CW1(T) (=KCTC 13142(T) =JCM 14730(T)).
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Keun Sik
authors: KS
authors: Baik
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Seong Chan
authors: SC
authors: Park
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Ho Jun
authors: HJ
authors: Kim
authors: Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong, Daejeon 305-333, Republic of Korea.
authors: Kang Hyun
authors: KH
authors: Lee
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Chi Nam
authors: CN
authors: Seong
mesh_terms: D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D006601:Hexoses / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000145:classification; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D056910:Republic of Korea
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006601:Hexoses; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002374:Catalase