title: Molecular characterization of Giardia duodenalis isolated from Semai Pahang Orang Asli (Peninsular Malaysia aborigines).
abstract: This study was conducted to determine the genotypes of Giardia duodenalis isolated from human faecal samples at Pos Betau, Pahang, Malaysia. Faecal specimens were collected and examined for G. duodenalis cysts using Trichrome staining techniques. Molecular identification was carried out by the amplification of a region of the small subunit of the nuclear ribosomal RNA (SSU rRNA) gene using nested PCR and subsequent sequencing. The sequences from 15 isolates from G. duodenalis were subjected to phylogenetic analysis (including appropriate outgroups) using the neighbor-joining and maximum parsimony methods. The trees identified G. duodenalis assemblages A and B, with a predominance of assemblage B. The predominance of anthroponotic genotypes indicates the possibility of anthroponotic transmission of these protozoa in this Semai Pahang Orang Asli community.
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. alsharaby9@yahoo.com
authors: A K Mohammed
authors: AK
authors: Mahdy
authors: Johari
authors: J
authors: Surin
authors: A
authors: A
authors: Mohd-Adnan
authors: K-L
authors: KL
authors: Wan
authors: Y A L
authors: YA
authors: Lim
mesh_terms: D000818:Animals; D044466:Asian People; D005243:Feces / Q000469:parasitology; D005838:Genotype; D005872:Giardia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000208:ethnology* / Q000469:parasitology; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012424:Rural Population; D017422:Sequence Analysis, DNA
chemical_list: D016053:RNA, Protozoan; D012335:RNA, Ribosomal