Proteome analysis of chloroplast proteins in stage albinism line of winter wheat (triticum aestivum) FA85.
The "stage albinism line of winter wheat" FA85 was a specific natural mutant strain on leaf color. This physiological mutation was controlled by cytogene. In order to reveal the genetic and biochemical mechanism of albinism, 2-DE was used to investigate the difference of chloroplast protein expression pattern between FA85 and its parent wheat Aibian 1. From the results of 2-DE gels analysis, approximately 683 spots were detected on each gel, and 57 spots were expressed differently at least two-fold. Using MALDI-TOF/TOF MS, 14 of 57 spots were identified, which could be categorized into four classes: carbon metabolism, energy metabolism, defense/stress response and signal transduction. Compared with the parent wheat, the expression of ATPase-gamma and GP1-alpha was up-regulated in FA85, and of other proteins was down-regulated. Together, we concluded that the expression of chloroplast proteins had changed obviously in FA85, which might be related to the leaf color mutant.
Hou
Dian-Yun
DY
College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
Xu
Hong
H
Du
Guang-Yuan
GY
Lin
Jun-Tang
JT
Duan
Min
M
Guo
Ai-Guang
AG
D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D053858:Metabolic Networks and Pathways / Q000502:physiology; D010858:Pigmentation / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome