Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring.
A thermophilic bacterium, designated strain CR11(T), was isolated from a filamentous sample collected from a terrestrial hot spring on the south-western foothills of the Rincón volcano in Costa Rica. The Gram-negative cells are approximately 2.4-3.9 microm long and 0.5-0.6 microm wide and are motile rods with polar flagella. Strain CR11(T) grows between 65 and 85 degrees C (optimum 75 degrees C, doubling time 4.5 h) and between pH 4.8 and 7.8 (optimum pH 5.9-6.5). The isolate grows chemolithotrophically with S(0), S(2)O(2)(3)(-) or H(2) as the electron donor and with O(2) (up to 16 %, v/v) as the sole electron acceptor. The isolate can grow on mannose, glucose, maltose, succinate, peptone, Casamino acids, starch, citrate and yeast extract in the presence of oxygen (4 %) and S(0). Growth occurs only at NaCl concentrations below 0.4 % (w/v). The G+C content of strain CR11(T) is 40.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence places the strain as a close relative of Thermocrinis ruber OC 1/4(T) (95.7 % sequence similarity). Based on phylogenetic and physiological characteristics, we propose the name Thermocrinis minervae sp. nov., with CR11(T) (=DSM 19557(T) =ATCC BAA-1533(T)) as the type strain.
Caldwell
Sara L
SL
Department of Biology, Portland State University, PO Box 751, Portland, OR 97207-0751, USA.
Liu
Yitai
Y
Department of Biology, Portland State University, PO Box 751, Portland, OR 97207-0751, USA.
Ferrera
Isabel
I
Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA, CSIC, Passeig Marítim de la Barceloneta 37-49, E-08003 Barcelona, Catalunya, Spain.
Beveridge
Terry
T
Department of Microbiology, College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Reysenbach
Anna-Louise
AL
Department of Biology, Portland State University, PO Box 751, Portland, OR 97207-0751, USA.
D001482:Base Composition; D003364:Costa Rica; D004269:DNA, Bacterial / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D006859:Hydrogen / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D013455:Sulfur / Q000378:metabolism; D014871:Water Microbiology
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013455:Sulfur; D006859:Hydrogen