title: Interaction between superantigen and T-cell receptor Vbeta element determines levels of superantigen-dependent cell-mediated cytotoxicity of CD8(+) T cells in induction and effector phases.
abstract: Specific superantigens activate different T-cell fractions with distinct TCR V beta elements in association with MHC class II molecules and also induce SDCC against MHC class II(+) target cells. In the present study, to determine whether the responsiveness of each CD8(+) T-cell fraction expressing a different TCR V beta element is primarily determined by the TCR V beta, we compared the levels of proliferation and SDCC in V beta3(+) and V beta11(+) T cells upon stimulation with SEA. Upon stimulation with SEA(wt), the levels of proliferation were higher in V beta3(+) T cells than in V beta11(+) T cells. The levels of SDCC were also higher for the combination of V beta3(+) T cells and SEA(wt) than for the combination of V beta11(+) T cells and SEA(wt) during both the induction phase and the effector phase. In addition, upon stimulation with SEA(m), the levels of proliferation were higher in V beta11(+) T cells than in V beta3(+) T cells. And then, the levels of SDCC were also higher for the combination of V beta11(+) T cells and SEA(m) than for the combination of Vbeta3(+) T cells and SEA(m) during both the induction phase and the effector phase. These results suggest that the SAG-responsiveness of each CD8(+) T-cell fraction expressing a different TCR V beta element is primarily determined by the interaction between the TCR V beta element and the SAG.
authors: Department of Microbiology and Immunology, Tokyo Women's Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors: Zhong-Juan
authors: ZJ
authors: Li
authors: Katsuhiko
authors: K
authors: Omoe
authors: Kunihiro
authors: K
authors: Shinagawa
authors: Junji
authors: J
authors: Yagi
authors: Ken'ichi
authors: K
authors: Imanishi
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013203:Staphylococcal Infections / Q000276:immunology* / Q000382:microbiology; D013210:Staphylococcus / Q000235:genetics / Q000276:immunology*; D018089:Superantigens / Q000235:genetics / Q000276:immunology*
chemical_list: D004768:Enterotoxins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C026066:enterotoxin A, Staphylococcal