title: Haladaptatus litoreus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haladaptatus.
abstract: Two extremely halophilic archaea, strains RO1-28(T) and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg(2+) and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0-8.5 and a temperature range of 25-55 degrees C, with optimal pH of 7.0 and optimal temperature of 37-40 degrees C. Based on 16S rRNA gene sequence analysis, strains RO1-28(T) and RO1-22 were closely related to Haladaptatus paucihalophilus, the single species of the genus Haladaptatus, with similarities of 94.0-95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of Haladaptatus paucihalophilus JCM 13897(T). Both strains RO1-28(T) and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA-DNA hybridization value between the two strains was more than 70 % (92 %) and both strains showed low levels of DNA-DNA relatedness (32 % and 33 %) with Haladaptatus paucihalophilus JCM 13897(T). It was concluded that strains RO1-28(T) and RO1-22 represent a novel species of the genus Haladaptatus, for which the name Haladaptatus litoreus sp. nov. is proposed. The type strain is RO1-28(T) (=CGMCC 1.7737(T) =JCM 15771(T)).
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors: Heng-Lin
authors: HL
authors: Cui
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors: Fang-Fang
authors: FF
authors: Sun
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors: Xia
authors: X
authors: Gao
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors: Ying
authors: Y
authors: Dong
authors: Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
authors: Xue-Wei
authors: XW
authors: Xu
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Yu-Guang
authors: YG
authors: Zhou
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Hong-Can
authors: HC
authors: Liu
authors: Department of Plant and Environmental Sciences, The Institute of Life Sciences, and The Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel.
authors: Aharon
authors: A
authors: Oren
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Pei-Jin
authors: PJ
authors: Zhou
mesh_terms: D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006216:Halobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity; D013696:Temperature
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride