Amnesic shellfish poisoning toxin stimulates the transcription of CYP1A possibly through AHR and ARNT in the liver of red sea bream Pagrus major.
To investigate the role of detoxification-related liver genes in amnesic shellfish poisoning toxin metabolism, red sea bream Pagrus major were exposed to domoic acid (DA, 2mugg(-1) wet weight) for 24h. Hepatic mRNA expression levels of AHR, ARNT, CYP1 and GSTs were determined by semi-quantitative RT-PCR. The cytosolic factors aryl hydrocarbon receptor (AHR) and aryl hydrocarbon receptor nuclear translocator (ARNT) mRNA levels of DA exposure group were substantially enhanced by 113.3% and 90.9%, respectively. Consistent with this result, the phase I xenobiotic metabolizing enzyme (XME) cytochrome P-450 1A (CYP1A) was significantly induced. In contrast, the transcriptions of three major phase II XME glutathione S-transferases as well as heat shock protein 70 were not significantly affected by DA exposure. These results suggest a possible role of CYP1A after DA exposure in the toxin metabolism of marine fish, possibly through the AHR/ARNT signaling pathway.
Wang
Lin
L
College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Liang
Xu-Fang
XF
Zhang
Wen-Bing
WB
Mai
Kang-Sen
KS
Huang
Yan
Y
Shen
Dan
D
D000647:Amnesia / Q000139:chemically induced / Q000378:metabolism; D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000145:classification / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D019363:Cytochrome P-450 CYP1A1 / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008387:Marine Toxins / Q000633:toxicity*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D021541:Sea Bream / Q000235:genetics / Q000378:metabolism*; D057096:Shellfish Poisoning; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D008387:Marine Toxins; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D014157:Transcription Factors; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D019363:Cytochrome P-450 CYP1A1; C012301:domoic acid; D007608:Kainic Acid