Malarial hemozoin is a Nalp3 inflammasome activating danger signal.
BACKGROUND
Characteristic symptoms of malaria include recurrent fever attacks and neurodegeneration, signs that are also found in patients with a hyperactive Nalp3 inflammasome. Plasmodium species produce a crystal called hemozoin that is generated by detoxification of heme after hemoglobin degradation in infected red blood cells. Thus, we hypothesized that hemozoin could activate the Nalp3 inflammasome, due to its particulate nature reminiscent of other inflammasome-activating agents.


RESULTS
We found that hemozoin acts as a proinflammatory danger signal that activates the Nalp3 inflammasome, causing the release of IL-1beta. Similar to other Nalp3-activating particles, hemozoin activity is blocked by inhibiting phagocytosis, K(+) efflux and NADPH oxidase. In vivo, intraperitoneal injection of hemozoin results in acute peritonitis, which is impaired in Nalp3-, caspase-1- and IL-1R-deficient mice. Likewise, the pathogenesis of cerebral malaria is dampened in Nalp3-deficient mice infected with Plasmodium berghei sporozoites, while parasitemia remains unchanged.


CONCLUSIONS
The potent pro-inflammatory effect of hemozoin through inflammasome activation may possibly be implicated in plasmodium-associated pathologies such as cerebral malaria.
Dostert
Catherine
C
Department of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Guarda
Greta
G
Romero
Jackeline F
JF
Menu
Philippe
P
Gross
Olaf
O
Tardivel
Aubry
A
Suva
Mario-Luca
ML
Stehle
Jean-Christophe
JC
Kopf
Manfred
M
Stamenkovic
Ivan
I
Corradin
Giampietro
G
Tschopp
Jurg
J
D000818:Animals; D006420:Hemeproteins / Q000502:physiology*; D053583:Interleukin-1beta / Q000378:metabolism; D051379:Mice; D010587:Phagocytosis; D010962:Plasmodium berghei / Q000502:physiology*; D015800:Protozoan Proteins / Q000502:physiology*
D006420:Hemeproteins; D053583:Interleukin-1beta; D015800:Protozoan Proteins; C020753:hemozoin