title: NADH dehydrogenase subunit-2 237 Leu/Met polymorphism modulates the effects of coffee consumption on the risk of hypertension in middle-aged Japanese men.
abstract: BACKGROUND
  Habitual coffee consumption has been reported to lower blood pressure in the Japanese population. The NADH dehydrogenase subunit-2 237 leucine/methionine (ND2-237 Leu/Met) polymorphism is associated with longevity and modifies the effects of alcohol consumption on blood pressure in the Japanese population. The objective of this study was to determine whether this polymorphism also modifies the effects of coffee consumption on blood pressure or the risk of hypertension in middle-aged Japanese men.
  
  
  METHODS
  A total of 398 men (mean age +/- standard deviation, 53.8 +/- 7.8 years) were selected from among individuals visiting the hospital for regular medical check-ups. Hypertension was defined as a systolic blood pressure > or =140 mm Hg, diastolic blood pressure > or =90 mm Hg, or antihypertensive drug treatment. Polymerase chain reaction-restriction fragment length polymorphism using the restriction enzyme AluI was performed to determine ND2-237 Leu/Met genotype.
  
  
  RESULTS
  In subjects with ND2-237Leu, coffee consumption was significantly and negatively associated with diastolic blood pressure (P = 0.007). The odds ratio (OR) for hypertension was significantly lower in subjects with ND2-237Leu who consumed 2 or 3 cups of coffee per day than in those who consumed less than 1 cup of coffee per day (OR, 0.517; 95% confidence interval [CI], 0.276 to 0.968; P = 0.039). After adjustment, the OR remained significant (OR = 0.399; 95% CI, 0.184 to 0.869; P = 0.020). Moreover, after adjustment, the OR was significantly lower in subjects with ND2-237Leu who consumed more than 4 cups of coffee per day than in those who consumed less than 1 cup of coffee per day (OR, 0.246; 95% CI, 0.062 to 0.975; P = 0.046). However, the association between ND2-237Met genotype and hypertension did not depend on coffee consumption.
  
  
  CONCLUSIONS
  The present results suggest that the ND2-237 Leu/Met polymorphism modulates the effects of coffee consumption on hypertension risk in middle-aged Japanese men.
authors: Department of Public Health, Showa University School of Medicine, Tokyo, Japan. akokaze@med.showa-u.ac.jp
authors: Akatsuki
authors: A
authors: Kokaze
authors: Mamoru
authors: M
authors: Ishikawa
authors: Naomi
authors: N
authors: Matsunaga
authors: Kanae
authors: K
authors: Karita
authors: Masao
authors: M
authors: Yoshida
authors: Tadahiro
authors: T
authors: Ohtsu
authors: Takako
authors: T
authors: Shirasawa
authors: Hideaki
authors: H
authors: Sekii
authors: Taku
authors: T
authors: Ito
authors: Teruyoshi
authors: T
authors: Kawamoto
authors: Yutaka
authors: Y
authors: Takashima
mesh_terms: D001794:Blood Pressure / Q000235:genetics; D003069:Coffee / Q000009:adverse effects; D006801:Humans; D006973:Hypertension / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology; D007564:Japan / Q000453:epidemiology; D007930:Leucine / Q000235:genetics*; D008297:Male; D008715:Methionine / Q000235:genetics*; D008875:Middle Aged; D009245:NADH Dehydrogenase / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D003069:Coffee; D008715:Methionine; D009245:NADH Dehydrogenase; C521326:NADH dehydrogenase subunit 2, human; D007930:Leucine