Development of rapid diagnostic reagents for measles.
Measles diagnosis has thus far been based on clinical symptoms in daily clinical practice. However, atypical cases are not uncommon; a simple and rapid method of measles diagnosis is clinically required. Lateral flow-based rapid diagnosis reagents are widely used for point-of-care testing in Japanese clinics, because it does not require special skills and facilities. We have developed a rapid diagnostic reagent for measles that employs the lateral flow method. The lower limit of detection of this reagent was almost the same for recombinant proteins of wild-type and vaccine strain, at 5.1 x 10(3) copies/test. This lower limit of detection and the specificity of this reagent suggest its efficacy for the diagnosis of measles infection.
Hasegawa
Takehiro
T
Department of Virology III, National Institute of Infectious Disease, Tokyo, Japan. Hasegawa.Takehiro@sysmex.co.jp
Asaeda
Ayumi
A
Hamaguchi
Yukio
Y
Numazaki
Kei
K
D000818:Animals; D002624:Chemistry, Clinical / Q000295:instrumentation* / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005838:Genotype; D047468:Immunoprecipitation; D007564:Japan; D008457:Measles / Q000175:diagnosis* / Q000276:immunology*; D008458:Measles Vaccine / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000378:metabolism; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D000939:Epitopes; D008458:Measles Vaccine; D012367:RNA, Viral; D011994:Recombinant Proteins