title: Isomeric oligosaccharides analyses using negative-ion electrospray ionization ion mobility spectrometry combined with collision-induced dissociation MS/MS.
abstract: Ion mobility-mass spectrometry (IM-MS) was applied to analyses of isomeric oligosaccharides linked by alpha1-4, alpha1-6, beta1-3, and beta1-4 glycosyl linkages. Negative-ion electrospray ionization (ESI) quadrupole IM time-of-flight mass spectrometry allowed the combination of IM separation and collision-induced dissociation (CID) MS/MS product ion analysis. Multimer formations of hexa-saccharide linkage isomers differ from each other, and their molecular shapes were analyzed by ion mobility spectrometry (IMS). The product ion spectra of the oligosaccharide isomers were measured by negative-ion CID-MS/MS for each IM separated peak. The spectrum for each isomer was distinct, and their corresponding linkage structures were identified by MS/MS analysis.
authors: SUNTORY Institute for Bioorganic Research, Mishima, Osaka, Japan. yumagaki@sunbor.or.jp
authors: Tohru
authors: T
authors: Yamagaki
authors: Akihiro
authors: A
authors: Sato
mesh_terms: D007536:Isomerism; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009844:Oligosaccharides