title: Connexin expression pattern in the endometrium of baboons is influenced by hormonal changes and the presence of endometriotic lesions.
abstract: Experimentally induced endometriosis in baboons serves as an elegant model to discriminate between endometrial genes which are primarily associated with normal endometrial function and those that are changed by the presence of endometriotic lesions. Since connexin genes are characteristic of the hormonally regulated differentiation of the endometrium, we have examined connexin expression in baboon endometrium to delineate if they are altered in response to the presence of endometriotic lesions. Connexin expression in the endometrium of cycling baboons is similar to that of the human endometrium with Connexin(Cx)43 being primarily seen in the stromal compartment and Cx26 and Cx32 being present predominantly in the epithelium. Although Cx32 is up-regulated during the secretory phase, Cx26 and Cx43 are down-regulated. In the baboon model of induced endometriosis a change in connexin pattern was evident in the presence of endometriotic lesions. In the secretory phase, Cx26 and Cx32 are no longer present in the epithelium but Cx26 is now observed primarily in the stromal cells. Infusion of chorionic gonadotrophin in a manner that mimics blastocyst transit in utero failed to rescue the aberrant stromal expression of Cx26 that is associated with the presence of endometriotic lesions suggesting an impairment of the implantation process. The altered connexin pattern coupled with a loss of the channel protein in the epithelium and a gain of Cx26 in the stromal compartment suggests that the presence of lesions changes the uterine environment and thereby the differentiation programme. This aberrant expression of connexins may be an additional factor that contributes to endometriosis-associated infertility.
authors: Institute of Molecular Biology, University of Duisburg-Essen, 45122 Essen, Germany. elke.winterhager@uk-essen.de
authors: E
authors: E
authors: Winterhager
authors: R
authors: R
authors: Grümmer
authors: P A
authors: PA
authors: Mavrogianis
authors: C J P
authors: CJ
authors: Jones
authors: J M
authors: JM
authors: Hastings
authors: A T
authors: AT
authors: Fazleabas
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000378:metabolism; D000072259:Connexin 26; D018031:Connexin 43 / Q000378:metabolism; D017630:Connexins / Q000378:metabolism*; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D008597:Menstrual Cycle / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction
chemical_list: D006063:Chorionic Gonadotropin; D018031:Connexin 43; D017630:Connexins; C000607042:GJB2 protein, human; D000072259:Connexin 26