title: A single nucleotide polymorphism analysis of the LAMA1 gene in Japanese patients with high myopia.
abstract: Although a myopia susceptibility gene has not yet been elucidated, ten candidate regions (MYP1-MYP10) have been associated with myopia by linkage analysis employing large pedigrees. We report herein on the results of our analysis pertaining to polymorphisms of LAMA1 (alpha subunit of laminin), a promising candidate gene for high myopia present in the MYP2 region of Japanese subjects with high myopia. Three hundred and thirty Japanese subjects with high myopia at a level of greater than -9.25 D and ethnically and sex matched 330 normal controls without high myopia was enrolled in this study. The thirteen SNPs located on the LAMA1 gene were analyzed using PCR and SNP-specific fluorogenic probes. Two of the SNPs were monomorphic and none of the 11 SNPs showed statistically significant association with high myopia in the Japanese population. There is no convincing evidence to prove a connection between nucleotide sequence variations in LAMA1 and high myopia. The pairwise linkage disequilibrium (LD) mapping disclosed a strong value (D' > 0.8) and narrow ranged block within these SNPs.
authors: Department of Ophthalmology, Yokohama City University School of Medicine, Kanagawa, Japan.
authors: Sayaka
authors: S
authors: Sasaki
authors: Masao
authors: M
authors: Ota
authors: Akira
authors: A
authors: Meguro
authors: Ritsuko
authors: R
authors: Nishizaki
authors: Eiichi
authors: E
authors: Okada
authors: Jeewon
authors: J
authors: Mok
authors: Tetusya
authors: T
authors: Kimura
authors: Akira
authors: A
authors: Oka
authors: Yoshihiko
authors: Y
authors: Katsuyama
authors: Shigeaki
authors: S
authors: Ohno
authors: Hidetoshi
authors: H
authors: Inoko
authors: Nobuhisa
authors: N
authors: Mizuki