title: Identification of a new nucleopolyhedrovirus from naturally-infected Condylorrhiza vestigialis (Guenée) (Lepidoptera: Crambidae) larvae on poplar plantations in South Brazil.
abstract: A baculovirus was isolated from larvae of Condylorrhiza vestigialis (Guenée) (Lepidoptera: Crambidae), a pest of a forest species known as Poplar (family Salicaceae, genus: Populus) with high economic value. Electron microscopy analysis of the occlusion body obtained from diseased larvae showed polyhedra containing multiple nucleocapsids per envelope. This baculovirus was thus named Condylorrhiza vestigialis multiple nucleopolyhedrovirus (CoveMNPV) and characterized by its DNA restriction endonuclease pattern, polyhedral protein, viral protein synthesis, and infectivity in insect cell lines. Restriction endonuclease profiles of viral DNA digested with five restriction enzymes were obtained and the CoveMNPV genome size was estimated to be 81+/-2.5 kbp. The isolation of the polyhedra (OBs) was done from the crude extract of infected larvae by ultracentrifugation through sucrose gradients. These viral particles were analyzed by denaturing polyacrylamide gel electrophoresis (SDS-PAGE), which showed a strong band with approximately 33 kDa, corresponding to the main protein of the occlusion bodies (polyhedrin). Also, a similar band was observed for CoveMNPV infected Spodoptera frugiperda cells (SF-21 AE) pulse-labeled with [(35)S] methionine and fractionated by SDS-PAGE. Of the four insect cell lines tested for susceptibility to CoveMNPV infection, the SF-21 AE was the most susceptible with occlusion bodies produced in most of the inoculated cells. This is the first record of an NPV from C. vestigialis.
authors: Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica W5 Norte Final, 70770-917 Brasília-DF, Brazil. elita@cenargen.embrapa.br
authors: Maria Elita B
authors: ME
authors: Castro
authors: Zilda Maria A
authors: ZM
authors: Ribeiro
authors: Ana Cláudia B
authors: AC
authors: Santos
authors: Marlinda L
authors: ML
authors: Souza
authors: Edilene B
authors: EB
authors: Machado
authors: Nilton J
authors: NJ
authors: Sousa
authors: Flávio
authors: F
authors: Moscardi
mesh_terms: D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D007300:Insect Control; D007814:Larva / Q000648:ultrastructure / Q000821:virology*; D009036:Moths / Q000648:ultrastructure / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D000077183:Occlusion Body Matrix Proteins; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000469:parasitology*; D032107:Populus; D015678:Viral Structural Proteins / Q000032:analysis / Q000235:genetics
chemical_list: D004279:DNA, Viral; D000077183:Occlusion Body Matrix Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus