Association of microsatellite polymorphisms of the GPDS1 locus with normal tension glaucoma in the Japanese population.
BACKGROUND
To investigate whether the GPDS1 locus, a potential causative locus of pigment-dispersion syndrome, is associated with normal-tension glaucoma (NTG) in Japanese patients.


METHODS
We used polymerase chain reaction amplification with sequence-specific primers to analyze 20 polymorphic microsatellite markers in and around the GPDS1 locus with an automated DNA analyzer and automated fragment detection by fluorescent-based technology. The DNA samples used for these analyses were obtained from ethnicity- and gender-matched patients, including 141 Japanese patients with NTG and 101 healthy controls. Patients exhibiting a comparatively early onset were selected as this suggests that genetic factors may show stronger involvement.


RESULTS
One allele of D7S2462 exhibited a frequency that was significantly decreased in NTG cases compared to controls (P = 0.0013, Pc = 0.019, OR = 0.48, 95% CI = 0.30-0.75). Alleles at another six microsatellite loci were positively or negatively associated with NTG, but these associations did not retain statistical significance after Bonferroni correction (P < 0.05, Pc > 0.05).


CONCLUSIONS
Our study showed a significant association between the GPDS1 locus and NTG, suggesting that there may be some genetic risk factor(s) in the development of NTG.
Nakamura
Kayo
K
Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Kanagawa, Japan.
Ota
Masao
M
Meguro
Akira
A
Nomura
Naoko
N
Kashiwagi
Kenji
K
Mabuchi
Fumihiko
F
Iijima
Hiroyuki
H
Kawase
Kazuhide
K
Yamamoto
Tetsuya
T
Nakamura
Makoto
M
Negi
Akira
A
Sagara
Takeshi
T
Nishida
Teruo
T
Inatani
Masaru
M
Tanihara
Hidenobu
H
Aihara
Makoto
M
Araie
Makoto
M
Fukuchi
Takeo
T
Abe
Haruki
H
Higashide
Tomomi
T
Sugiyama
Kazuhisa
K
Kanamoto
Takashi
T
Kiuchi
Yoshiaki
Y
Iwase
Aiko
A
Ohno
Shigeaki
S
Inoko
Hidetoshi
H
Mizuki
Nobuhisa
N