Addressing the malaria drug resistance challenge using flow cytometry to discover new antimalarials.
A new flow cytometry method that uses an optimized DNA and RNA staining strategy to monitor the growth and development of the Plasmodium falciparum strain W2mef has been used in a pilot study and has identified Bay 43-9006 1, SU 11274 2, and TMC 125 5 as compounds that exhibit potent (<1 microM) overall and ring stage in vitro antimalarial activity.
Grimberg
Brian T
BT
Center for Global Health & Diseases, Case Western Reserve University, Cleveland, OH 44106-7286, USA.
Jaworska
Maria M
MM
Hough
Lindsay B
LB
Zimmerman
Peter A
PA
Phillips
James G
JG
D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000032:analysis*; D055808:Drug Discovery; D004351:Drug Resistance; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry / Q000191:economics / Q000379:methods*; D016778:Malaria, Falciparum / Q000188:drug therapy*; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development; D016053:RNA, Protozoan / Q000032:analysis*; D013329:Structure-Activity Relationship
D000962:Antimalarials; D016054:DNA, Protozoan; D016053:RNA, Protozoan