title: Recovery of cell nuclei from 15,000 years old mammoth tissues and its injection into mouse enucleated matured oocytes.
abstract: Here, we report the recovery of cell nuclei from 14,000-15,000 years old mammoth tissues and the injection of those nuclei into mouse enucleated matured oocytes by somatic cell nuclear transfer (SCNT). From both skin and muscle tissues, cell nucleus-like structures were successfully recovered. Those nuclei were then injected into enucleated oocytes and more than half of the oocytes were able to survive. Injected nuclei were not taken apart and remained its nuclear structure. Those oocytes did not show disappearance of nuclear membrane or premature chromosome condensation (PCC) at 1 hour after injection and did not form pronuclear-like structures at 7 hours after injection. As half of the oocytes injected with nuclei derived from frozen-thawed mouse bone marrow cells were able to form pronuclear-like structures, it might be possible to promote the cell cycle of nuclei from ancient animal tissues by suitable pre-treatment in SCNT. This is the first report of SCNT with nuclei derived from mammoth tissues.
authors: Institute of Advanced Technology, Kinki University, Wakayama, Japan. iritani@waka.kindai.ac.jp
authors: Hiromi
authors: H
authors: Kato
authors: Masayuki
authors: M
authors: Anzai
authors: Tasuku
authors: T
authors: Mitani
authors: Masahiro
authors: M
authors: Morita
authors: Yui
authors: Y
authors: Nishiyama
authors: Akemi
authors: A
authors: Nakao
authors: Kenji
authors: K
authors: Kondo
authors: Petr A
authors: PA
authors: Lazarev
authors: Tsuyoshi
authors: T
authors: Ohtani
authors: Yasuyuki
authors: Y
authors: Shibata
authors: Akira
authors: A
authors: Iritani
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D004606:Elephants; D005260:Female; D005580:Fossils; D007267:Injections; D051379:Mice; D008969:Molecular Sequence Data; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D055110:Radiometric Dating; D013997:Time Factors