Development and validation of a liquid chromatography mass spectrometry assay for the simultaneous quantification of methadone, cocaine, opiates and metabolites in human umbilical cord.
A liquid chromatography mass spectrometric selected reaction monitoring mode (SRM) method for methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), cocaine, benzoylecgonine (BE), 6-acetylmorphine, morphine and codeine quantification in human umbilical cord was developed and fully validated. Analytes were extracted from homogenized tissue (1g) by solid phase extraction. Linearity was 2.5-500ng/g, except for methadone (10-2000ng/g). Method imprecision was <12.7%CV with analytical recovery 85.9-112.7%, extraction efficiency >59.2%, matrix effect 4.5-39.5%, process efficiency 48.6-92.6% and stability >84.6%. Analysis of an umbilical cord following controlled methadone administration and illicit drug use contained in ng/g, 40.3 morphine, 3.6 codeine, 442 BE, 186 methadone and 45.9 EDDP.
de Castro
Ana
A
Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
Concheiro
Marta
M
Shakleya
Diaa M
DM
Huestis
Marilyn A
MA
D002853:Chromatography, Liquid / Q000379:methods*; D003042:Cocaine / Q000032:analysis*; D004355:Drug Stability; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D013058:Mass Spectrometry / Q000379:methods*; D008691:Methadone / Q000032:analysis*; D053610:Opiate Alkaloids / Q000032:analysis*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014470:Umbilical Cord / Q000737:chemistry*
D053610:Opiate Alkaloids; D003042:Cocaine; D008691:Methadone