title: Genome-wide screens: novel mechanisms in colicin import and cytotoxicity.
abstract: Only two new genes (fkpA and lepB) have been identified to be required for colicin cytotoxicity in the last 25 years. Genome-wide screening using the 'Keio collection' to test sensitivity to colicins (col) A, B, D, E1, E2, E3, E7 and N from groups A and B, allowed identification of novel genes affecting cytotoxicity and provided new information on mechanisms of action. The requirement of lipopolysaccharide for colN cytotoxicity resides specifically in the lipopolysaccharide inner-core and first glucose. ColA cytotoxicity is dependent on gmhB and rffT genes, which function in the biosynthesis of lipopolysaccharide and enterobacterial common antigen. Of the tol genes that function in the cytoplasmic membrane translocon, colE1 requires tolA and tolR but not tolQ for activity. Peptidoglycan-associated lipoprotein, which interacts with the Tol network, is not required for cytotoxicity of group A colicins. Except for TolQRA, no cytoplasmic membrane protein is essential for cytotoxicity of group A colicins, implying that TolQRA provides the sole pathway for their insertion into/through the cytoplasmic membrane. The periplasmic protease that cleaves between the receptor and catalytic domains of colE7 was not identified, implying either that the responsible gene is essential for cell viability, or that more than one gene product has the necessary proteolysis function.
authors: Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907, USA.
authors: Onkar
authors: O
authors: Sharma
authors: Kirill A
authors: KA
authors: Datsenko
authors: Sara C
authors: SC
authors: Ess
authors: Mariya V
authors: MV
authors: Zhalnina
authors: Barry L
authors: BL
authors: Wanner
authors: William A
authors: WA
authors: Cramer
mesh_terms: D003087:Colicins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D015723:Gene Library; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016680:Genome, Bacterial
chemical_list: D003087:Colicins