Partial functional redundancy of MreB isoforms, MreB, Mbl and MreBH, in cell morphogenesis of Bacillus subtilis.
MreB proteins are bacterial actin homologues thought to have a role in cell shape determination by positioning the cell wall synthetic machinery. Many bacteria, particularly Gram-positives, have more than one MreB isoform. Bacillus subtilis has three, MreB, Mbl and MreBH, which colocalize in a single helical structure. We now show that the helical pattern of peptidoglycan (PG) synthesis in the cylindrical part of the rod-shaped cell is governed by the redundant action of the three MreB isoforms. Single mutants for any one of mreB isoforms can still incorporate PG in a helical pattern and generate a rod shape. However, after depletion of MreB in an mbl mutant (or depletion of all three isoforms) lateral wall PG synthesis was impaired and the cells became spherical and lytic. Overexpression of any one of the MreB isoforms overcame the lethality as well as the defects in lateral PG synthesis and cell shape. Furthermore, MreB and Mbl can associate with the peptidoglycan biosynthetic machinery independently. However, no single MreB isoform was able to support normal growth under various stress conditions, suggesting that the multiple isoforms are used to allow cells to maintain proper growth and morphogenesis under changing and sometimes adverse conditions.
Kawai
Yoshikazu
Y
Institute for Cell and Molecular Biosciences, Newcastle University, Medical School, Newcastle Upon Tyne NE2 4HH, UK.
Asai
Kei
K
Errington
Jeffery
J
D001412:Bacillus subtilis / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D050296:Microbial Viability; D009024:Morphogenesis; D009154:Mutation; D010457:Peptidoglycan / Q000096:biosynthesis*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D010457:Peptidoglycan; D020033:Protein Isoforms; C424044:mbl protein, Bacillus subtilis