title: Effect of desensitizing agents on dentin permeability.
abstract: OBJECTIVE
  To investigate the in vitro efficacy of two dentin desensitizing products at reducing liquid permeability through human dentin discs. The tested hypothesis was that the products, in spite of different chemical mechanisms were not different at reducing or eliminating flow through dentin discs.
  
  
  METHODS
  Dentin slices (1 mm thick) were prepared from 16 extracted human third molars and their permeability was indirectly recorded in a split chamber model, using a chemiluminescence technique, after EDTA treatment (control), after soaking with albumin, and after desensitizer application. Two products were studied: MS Coat, a self-curing resin-containing oxalate product, and Gluma Desensitizer, a glutaraldehyde/HEMA-based agent without initiator. The dentin slices were mounted between an upper chamber, filled with an aqueous solution of 1% potassium ferricyanide and 0.3% hydrogen peroxide, and a lower chamber filled with 1% sodium hydroxide solution and 0.02% luminol. The upper solution was pressurized, and upon contact with the luminol solution a photochemical signal was generated and recorded as a measure of permeability throughout two consecutive pressurizing cycles at 2.5 and 13 kPa (26 and 133 cm H2O), respectively.
  
  
  RESULTS
  The permeability of the control and albumin-soaked samples was similarly high. After application of the desensitizing agents, dentin permeability was reduced to virtually zero at both pressure levels (P < 0.001).
authors: Department of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors: Hiroshi
authors: H
authors: Ishihata
authors: Masafumi
authors: M
authors: Kanehira
authors: Tomoko
authors: T
authors: Nagai
authors: Werner J
authors: WJ
authors: Finger
authors: Hidetoshi
authors: H
authors: Shimauchi
authors: Masashi
authors: M
authors: Komatsu
mesh_terms: D003806:Dentin Permeability / Q000187:drug effects*; D003807:Dentin Sensitivity / Q000188:drug therapy*; D019234:Dentinal Fluid / Q000502:physiology; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D049449:Luminescence; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology*; D010070:Oxalates / Q000494:pharmacology*
chemical_list: D008689:Methacrylates; D010070:Oxalates; C121034:Pain-Free; C111486:gluma desensitizer; D005976:Glutaral