Description of Olleya aquimaris sp. nov., isolated from seawater, and emended description of the genus Olleya Mancuso Nichols et al. 2005.
A Gram-stain-negative, non-flagellated, motile (by gliding), yellow-pigmented, rod-shaped bacterial strain, designated L-4(T), was isolated from seawater of Baekdo harbour in the East Sea, Korea. Strain L-4(T) grew optimally at 37 degrees C, at pH 6.5-7.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain L-4(T) clustered with Olleya marilimosa CAM030(T), a member of the family Flavobacteriaceae. Strain L-4(T) exhibited 16S rRNA gene sequence similarity values of 97.2 % to O. marilimosa CAM030(T) and less than 95.8 % to other members of the family Flavobacteriaceae. Strain L-4(T) and O. marilimosa CIP 108537(T) contained MK-6 as the predominant menaquinone. The fatty acid and polar lipid profiles of strain L-4(T) were similar to those of O. marilimosa CIP 108537(T ). The DNA G+C content of strain L-4(T) was 35 mol% and DNA-DNA relatedness between strain L-4(T) and O. marilimosa CIP 108537(T) was 7 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, enable strain L-4(T) to be distinguished from O. marilimosa . On the basis of these data, strain L-4(T) is considered to represent a novel species of the genus Olleya for which the name Olleya aquimaris sp. nov. is proposed; the type strain is L-4(T) (=KCTC 22661(T) =CCUG 58074(T)). An emended description of the genus Olleya is also provided.
Lee
Soo-Young
SY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Park
Sooyeon
S
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S