Demequina salsinemoris sp. nov., isolated on agar media supplemented with ascorbic acid or rutin.
Three strains, KV-810(T), KV-811 and KV-816, were isolated from mangrove soil from a southern island in Japan on media supplemented with ascorbic acid or rutin. These strains contained l-ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan and DMK-9(H(4)) as the predominant menaquinone. The G+C content of the DNA was 70-72 mol%. These characteristics in combination with 16S rRNA gene sequence analysis revealed that the novel strains belonged to the genus Demequina. The DNA-DNA hybridization values showed that the three new strains belonged to the same species, a novel species of the genus Demequina. Therefore strains KV-810(T), KV-811 and KV-816 are proposed as representing a novel species, Demequina salsinemoris sp. nov. The type strain is KV-810(T) (=DSM 22060(T)=NBRC 105323(T)).
Matsumoto
Atsuko
A
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Nakai
Kengo
K
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Morisaki
Kurimi
K
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Ōmura
Satoshi
S
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Takahashi
Yōko
Y
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D000362:Agar; D001205:Ascorbic Acid / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012431:Rutin / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012431:Rutin; D000362:Agar; D001205:Ascorbic Acid