title: [Studies on the oxidation of LDL induced by Cu2 at different time by spectroscopic method].
abstract: Oxidized low density lipoprotein (oxLDL) is thought to be a key proatherogenic event. The oxidation of LDL induced by Cu+ at different time was studied by UV-Vis absorption spectra and circular dichroism (CD) spectra. It was shown that long oxidation time is accompanied by an increased MDA content and fluorescence intensity at 430 nm, which suggested new product. The intensity of UV absorption peak of LDL increased, while fluorescence and synchronous fluorescence intensities, which typified amino acids, decreased. CD data displayed that alpha-helical content of the protein decreased and the secondary structure changed. The results indicated that during the process of oxidation of LDL Cu2+ induced not only the product but also the change of the conformation of apoB-100.
authors: College of Bioengineering, Dalian University, Dalian 116622, China. sx_221@yahoo.com.cn
authors: Xia
authors: X
authors: Sun
authors: Qing-ping
authors: QP
authors: Liu
authors: Xue-fang
authors: XF
authors: Zheng
authors: Zhe
authors: Z
authors: Liu
authors: Ren-jun
authors: RJ
authors: Wang
authors: Zhen-yu
authors: ZY
authors: Huang
authors: Fan
authors: F
authors: Zhang
mesh_terms: D000042:Absorption; D002942:Circular Dichroism; D003300:Copper / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D008077:Lipoproteins, LDL; D008315:Malondialdehyde; D003300:Copper