Highly sensitive fetal goat tongue cell line for detection and isolation of foot-and-mouth disease virus.
A fetal goat cell line (ZZ-R 127) supplied by the Collection of Cell Lines in Veterinary Medicine of the Friedrich Loeffler Institute was examined for susceptibility to infection by foot-and-mouth disease (FMD) virus (FMDV) and by two other viruses causing clinically indistinguishable vesicular conditions, namely, the viruses of swine vesicular disease and vesicular stomatitis. Primary bovine thyroid (BTY) cells are generally the most sensitive cell culture system for FMDV detection but are problematic to produce, particularly for laboratories that infrequently perform FMD diagnostic tests and for those in countries where FMD is endemic that face problems in sourcing thyroid glands from FMD-negative calves. Strains representing all seven serotypes of FMDV could be isolated in ZZ-R 127 cells with a sensitivity that was considerably higher than that of established cell lines and within 0.5 log of that for BTY cells. The ZZ-R 127 cell line was found to be a sensitive, rapid, and convenient tool for the isolation of FMDV and a useful alternative to BTY cells for FMD diagnosis.
Brehm
K E
KE
Friedrich-Loeffler-Institute, Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald-Insel Riems, Germany. katharina.brehm@fli.bund.de
Ferris
N P
NP
Lenk
M
M
Riebe
R
R
Haas
B
B
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000254:growth & development* / Q000302:isolation & purification*; D006041:Goats; D012680:Sensitivity and Specificity; D018116:Vesiculovirus / Q000254:growth & development