A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene.
Calpains are a ubiquitous cytoplasmic cysteine protease, the activity of which is absolutely dependent on calcium. This proteolytic system degrades myofibrillar protein under post-mortem conditions and appears to be the primary enzyme in the postmortem tenderization process. In the present study a new single nucleotide polymorphism was found in the bovine CAPNS1 gene exon 11 coding for the 3'UTR. Transition C --> T at position 6536 was detected and identified using PCR-SSCP and DNA sequencing techniques, and then analysed with PCR-RFLP using MboII nuclease. The genotype frequencies and alleles distribution were studied in 190 bulls including, Charolaise, Hereford, Limousine, Simmental, Polish Red and Fresian breeds.
Juszczuk-Kubiak
E
E
Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzebiec, Poland. e.kubiak@ighz.pl
Flisikowski
K
K
Wicińska
K
K
D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D002154:Calpain / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D021122:Protein Subunits / Q000235:genetics*
D020413:3' Untranslated Regions; D021122:Protein Subunits; D002154:Calpain