Haladaptatus cibarius sp. nov., an extremely halophilic archaeon from seafood, and emended description of the genus Haladaptatus.
A novel, extremely halophilic archaeon, D43(T), was isolated from traditional salt-fermented seafood in Korea. The cells were Gram-negative-staining and motile. The strain grew at 15-50 degrees C, 10-30 % (w/v) NaCl and pH 6.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D43(T) is affiliated with the family Halobacteriaceae in the domain Archaea and had 95.5 % 16S rRNA gene sequence similarity with Haladaptatus paucihalophilus DX253(T). The sequence from strain D43(T) formed a clade with those from Hap. paucihalophilus regardless of which tree-generating algorithm was used. DNA-DNA hybridization experiments showed 25.8 % relatedness between the isolate and Hap. paucihalophilus KCTC 4006(T). Major lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two unidentified glycolipids. The DNA G+C content of the isolate was 56.5 mol%. On the basis of this polyphasic taxonomic study, strain D43(T) represents a novel species in the genus Haladaptatus, for which the name Haladaptatus cibarius sp. nov. is proposed. The type strain is D43(T) (=DSM 19505(T) =JCM 15962(T)).
Roh
Seong Woon
SW
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Lee
Myung-Lip
ML
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Bae
Jin-Woo
JW
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
D000818:Animals; D001482:Base Composition; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005285:Fermentation; D020459:Genes, rRNA; D005838:Genotype; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017747:Seafood / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012758:Shellfish / Q000382:microbiology*; D012965:Sodium Chloride; D013045:Species Specificity
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride