A comprehensive analysis of the soybean genes and proteins expressed under flooding stress using transcriptome and proteome techniques.
The inducible genes and proteins were analyzed using transcriptome and proteome techniques to explore the mechanisms underlying soybean response to flooding stress. Soybean seedlings were germinated for 2 days and subjected to flooding for 12 h, and the total RNAs and proteins were extracted from the root and hypocotyl. High-coverage gene expression profiling analysis as transcriptome technique was performed. Ninety-seven out of the 29,388 peaks observed demonstrated a greater than 25-fold change following 12 h of flood-induced stress. Furthermore, 34 proteins out of 799 proteins were changed by 12 h stress. Genes associated with alcohol fermentation, ethylene biosynthesis, pathogen defense, and cell wall loosening were significantly up-regulated. Hemoglobin, acid phosphatase, and Kunitz trypsin protease inhibitor were altered at both transcriptional and translational levels. Reactive oxygen species scavengers and chaperons were changed only at the translational level. It is suggested that the early response of soybean under flooding might be important stress adaptation to ensure survival against not only hypoxia but also the direct damage of cell by water.
Komatsu
Setsuko
S
National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp
Yamamoto
Ryo
R
Nanjo
Yohei
Y
Mikami
Yoji
Y
Yunokawa
Harunobu
H
Sakata
Katsumi
K
D000438:Alcohols / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors; D015180:Electrophoresis, Gel, Two-Dimensional; D005030:Ethylenes / Q000378:metabolism; D055868:Floods; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D018546:Hypocotyl / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013025:Glycine max / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D013997:Time Factors
D000438:Alcohols; D015853:Cysteine Proteinase Inhibitors; D005030:Ethylenes; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; C036216:ethylene; D005964:Glucosyltransferases