The evaluation of IL-12 concentration, PAF-AH, and PLA(2) activity in patients with type 1 diabetes treated with intensive insulin therapy.
OBJECTIVE
The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy.


METHODS
Studied parameters were measured in 81 patients, who were subdivided according to the HbA(1)c value, hsCRP concentration, and presence or absence of late complications.


RESULTS
PAF-AH activity was higher in the DM1 patients versus the control group (P=0.042). IL-12 concentration was the highest in subgroup with > or =3 mg/L hsCRP (P<0.05). Negative correlations were found for the IL-12 and age of patients and for apo A-I in the subgroup with poor metabolic control. In addition, positive correlation for hsCRP and PAF-AH activity in the subgroup with > or =3 mg/L CRP (P<0.05) was also found.


CONCLUSIONS
PAF-AH and IL-12 appear to be implicated in the development of a chronic inflammation in DM1. In addition, our results emphasize a protective role of apo A-I against an increase in IL-12 production.
Wegner
Małgorzata
M
Department of General Chemistry, Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, 6 Grunwaldzka St., Poznan, Poland. malgoweg@ump.edu.pl
Araszkiewicz
Aleksandra
A
Pioruńska-Mikołajczak
Anna
A
Zozulińska-Ziółkiewicz
Dorota
D
Wierusz-Wysocka
Bogna
B
Pioruńska-Stolzmann
Maria
M
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000097:blood*; D000328:Adult; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism; D005260:Female; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007328:Insulin / Q000627:therapeutic use*; D018664:Interleukin-12 / Q000097:blood*; D008297:Male; D054467:Phospholipases A2 / Q000097:blood*
D007004:Hypoglycemic Agents; D007328:Insulin; D018664:Interleukin-12; D054467:Phospholipases A2; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase