Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester.
Screening was carried out for microorganisms able to produce N-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing APM production was purified and identified as dipeptidase E.
Kira
Ikuo
I
AminoScience Laboratories, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan. ikuo_kira@ajinomoto.com
Asano
Yasuhisa
Y
Yokozeki
Kenzo
K
D001216:Asparagine / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D002384:Catalysis; D002954:Citrobacter / Q000201:enzymology; D004150:Dipeptidases / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012486:Salmonella typhimurium / Q000201:enzymology; D012763:Shigella flexneri / Q000201:enzymology
D011994:Recombinant Proteins; C034515:phenylalanine methyl ester; D010649:Phenylalanine; D001216:Asparagine; D004150:Dipeptidases