title: Use of polyamidoamine dendrimers to engineer BDNF-producing human mesenchymal stem cells.
abstract: We report the use of polyamidoamine (PAMAM-NH(2)) dendrimers along with other non-viral vehicles for the in vitro transfection of human bone marrow mesenchymal stem cells (hMSCs) and for engineering MSCs to secrete brain-derived neurotrophic factor (BDNF). Different generations of cationic polyamidoamine dendrimers (generations 3-6) were tested on HEK 293T cells. hMSCs were then transfected with PAMAM-NH(2) G4 dendrimers and Lipofectamine 2000, which elicited the expression of GFP reporter in around 6 and 20% of the cells, respectively. Both vehicles were then shown to elicit the expression of BDNF in MSCs from a bicistronic cassette. Non-virally induced neurotrophin expression may be a safe and easy method for adapting autologous stem cells for therapeutic treatment of diseases and neural system injuries.
authors: Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Akademicheskaya, 27, 220072, Minsk, Belarus. shakhbazau@gmail.com
authors: Antos
authors: A
authors: Shakhbazau
authors: Dzmitry
authors: D
authors: Shcharbin
authors: Ihar
authors: I
authors: Seviaryn
authors: Natalya
authors: N
authors: Goncharova
authors: Svetlana
authors: S
authors: Kosmacheva
authors: Mihail
authors: M
authors: Potapnev
authors: Barbara
authors: B
authors: Gabara
authors: Maxim
authors: M
authors: Ionov
authors: Maria
authors: M
authors: Bryszewska
mesh_terms: D000328:Adult; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D050091:Dendrimers / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D019076:Transgenes / Q000235:genetics
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D050091:Dendrimers; C104700:PAMAM Starburst; D004247:DNA