title: Role of sphingosine kinase 1 in allergen-induced pulmonary vascular remodeling and hyperresponsiveness.
abstract: BACKGROUND
  Immunologic processes might contribute to the pathogenesis of pulmonary arterial hypertension (PAH), a fatal condition characterized by progressive pulmonary arterial remodeling, increased pulmonary vascular resistance, and right ventricular failure. Experimental allergen-driven lung inflammation evoked morphologic and functional vascular changes that resembled those observed in patients with PAH. Sphingosine kinase 1 (SphK1) is the main pulmonary contributor to sphingosine-1-phosphate (S1P) synthesis, a modulator of immune and vascular functions.
  
  
  OBJECTIVE
  We sought to investigate the role of SphK1 in allergen-induced lung inflammation.
  
  
  METHODS
  SphK1-deficient mice and C57Bl/6 littermates (wild-type [WT] animals) were subjected to acute or chronic allergen exposure.
  
  
  RESULTS
  After 4 weeks of systemic ovalbumin sensitization and local airway challenge, airway responsiveness increased less in SphK1(-/-) compared with WT mice, whereas pulmonary vascular responsiveness was greatly increased and did not differ between strains. Acute lung inflammation led to an increase in eosinophils and mRNA expression for S1P phosphatase 2 and S1P lyase in lungs of WT but not SphK1(-/-) mice. After repetitive allergen exposure for 8 weeks, airway responsiveness was not augmented in SphK1(-/-) or WT mice, but pulmonary vascular responsiveness was increased in both strains, with significantly higher vascular responsiveness in SphK1(-/-) mice compared with that seen in WT mice. Increased vascular responsiveness was accompanied by remodeling of the small and intra-acinar arteries.
  
  
  CONCLUSIONS
  : The data support a role for SphK1 and S1P in allergen-induced airway inflammation. However, SphK1 deficiency increased pulmonary vascular hyperresponsiveness, which is a component of PAH pathobiology. Moreover, we show for the first time the dissociation between inflammation-induced remodeling of the airways and pulmonary vasculature.
authors: Department of Anatomy and Histology, Flinders University, Adelaide, Australia.
authors: Rainer V
authors: RV
authors: Haberberger
authors: Christoph
authors: C
authors: Tabeling
authors: Sue
authors: S
authors: Runciman
authors: Birgitt
authors: B
authors: Gutbier
authors: Peter
authors: P
authors: König
authors: Manfred
authors: M
authors: Andratsch
authors: Hartwig
authors: H
authors: Schütte
authors: Norbert
authors: N
authors: Suttorp
authors: Ian
authors: I
authors: Gibbins
authors: Martin
authors: M
authors: Witzenrath
mesh_terms: D000208:Acute Disease; D000485:Allergens / Q000276:immunology; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000276:immunology*; D002908:Chronic Disease; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D006976:Hypertension, Pulmonary / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008246:Lysophospholipids / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011651:Pulmonary Artery / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000276:immunology / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D000099157:Sphingosine Kinase
chemical_list: D000485:Allergens; D016207:Cytokines; D008246:Lysophospholipids; D010047:Ovalbumin; D017853:Phosphotransferases (Alcohol Group Acceptor); D012333:RNA, Messenger; D013110:Sphingosine; D000099157:Sphingosine Kinase; C060506:sphingosine 1-phosphate