Development of orthogonal two-dimensional hydrophilic interaction chromatography systems with the introduction of novel stationary phases.
Two different offline 2-D hydrophilic interaction chromatography (2D-HILIC/HILIC) systems have been developed. In the two systems, a click maltose column was used in the first dimension, an amide column or a click beta-CD column was used in the second dimension, respectively. Both of the systems were used for the analysis of very polar components in Carthamus tinctorius Linn., which is a traditional Chinese medicine. Excellent orthogonality and separation results were obtained in both 2D-HILIC/HILIC systems, while the peak capacity of the system based on click maltose and amide column was higher for its adoption of stationary phase with smaller particle size in the second dimension.
Liu
Yanming
Y
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, PR China.
Guo
Zhimou
Z
Feng
Jiatao
J
Xue
Xingya
X
Zhang
Feifang
F
Xu
Qing
Q
Liang
Xinmiao
X