title: A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8.
abstract: Plants are highly adapted to respond to a range of environmental stresses commonly by altering their gene expression and metabolism as a result of cell signalling which may be mediated by reactive oxygen species. The glycine-rich RNA-binding proteins ATGRP7 and ATGRP8 were rapidly upregulated in response to peroxide-induced oxidative stress and were amongst the most abundant RNA binding proteins isolated by oligo(dT) chromatography. The oligo(dT)-bound mRNP complexes were analysed proteomically, and were seen to contain potential isoforms of the ATGRP proteins; other proteins that contain an RNA Recognition Motif (RRM); and chloroplast RNA binding proteins. These findings suggest that ATGRP proteins have an evolutionarily conserved function in the regulation of gene expression at the posttranscriptional level in response to environmental stress.
authors: Faculty of Health and Life Sciences, University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, UK.
authors: Fabian
authors: F
authors: Schmidt
authors: Aline
authors: A
authors: Marnef
authors: Man-Kim
authors: MK
authors: Cheung
authors: Ian
authors: I
authors: Wilson
authors: John
authors: J
authors: Hancock
authors: Dorothee
authors: D
authors: Staiger
authors: Michael
authors: M
authors: Ladomery
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics* / Q000502:physiology; D040901:Proteomics / Q000379:methods; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000032:analysis* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D009838:Oligodeoxyribonucleotides; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C118496:GRP7 protein, Arabidopsis; C534507:GRP8 protein, Arabidopsis; C026452:messenger ribonucleoprotein; C027903:oligo (dT)