Ohr, an in vivo-induced gene in Actinobacillus pleuropneumoniae, is located on a genomic island and requires glutathione-S-transferase for activity.
Actinobacillus pleuropneumoniae is the causative agent of severe necrotizing pneumonia in swine. Previously, we identified the ohr gene encoding organic hydroperoxide reductase as specifically induced during infection of pigs, induced in vitro by organic peroxides but not other oxygen radicals, and present in A. pleuropneumoniae serotypes 1, 9 and 11 but not in other serotypes (Shea & Mulks, 2002). Through analysis of flanking genomic sequence, we identify a homologue of gst, which encodes glutathione-S-transferase, immediately downstream of ohr and demonstrate that ohr-gst confers low but uninducible Ohr activity to serotype 5. We further identify a genomic island of 9.3 kb, flanked by lysR and araC homologues, in serotypes 1, 9 and 11, which contains ohr and gst. In serotypes 2-8, 10 and 12, this region of the genome contains a 1.1-kb islet with a putative transposase flanked by lysR and araC.
Wolfram
Thaddeus J
TJ
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
Leveque
Rhiannon M
RM
Kastenmayer
Robin J
RJ
Mulks
Martha H
MH
D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D044404:Genomic Islands; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C447142:Ohr protein, Actinobacillus pleuropneumoniae; D010544:Peroxidases; D005982:Glutathione Transferase