title: An ancestral glutamine-dependent NAD(+) synthetase revealed by poor kinetic synergism.
abstract: NAD(+) synthetase catalyzes the formation of NAD(+) from ATP, nicotinic acid adenine dinucleotide and ammonia. Glutamine-dependent NAD(+) synthetase obtains ammonia through the hydrolysis of glutamine to glutamate, which takes place in the glutaminase domain. The ammonia is subsequently transported to the synthetase domain through an interdomain ammonia tunnel. NAD(+) synthetase from the thermophilic bacteria Thermotoga maritima was cloned and expressed. Steady-state kinetics and stoichiometric analysis of product formation revealed an enzyme that is significantly inefficient in the synchronization of the two active sites resulting in wasteful hydrolysis of glutamine and that is not specific for glutamine over ammonia. Phylogenetic analysis of glutamine-dependent NAD(+) synthetases identifies three main groups remotely related. The T. maritima NAD(+) synthetase's group is proposed to represent the ancestral group based on the phylogenetic analysis and on the kinetic characterizations. The phylogenetic results nicely correlate also with the degree of catalytic efficiency measured for M. tuberculosis, S. cerevisiae and T. maritima NAD(+) synthetases. Furthermore, the data here reported in combination with structural data available for glutamine-dependent NAD(+) synthetase lays the foundation for further investigation on the mechanism of active site coupling in these enzymes.
authors: Department of Chemistry and Biochemistry, Building 091, University of Maryland, College Park, MD 20742-2021, USA.
authors: Melissa
authors: M
authors: Resto
authors: Jason
authors: J
authors: Yaffe
authors: Barbara
authors: B
authors: Gerratana
mesh_terms: D019732:Amide Synthases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D005973:Glutamine / Q000378:metabolism; D006358:Hot Temperature; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D009243:NAD / Q000031:analogs & derivatives; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D020124:Thermotoga maritima / Q000201:enzymology
chemical_list: D005973:Glutamine; D009243:NAD; C018348:nicotinic acid adenine dinucleotide; D019732:Amide Synthases; C038479:NAD+ synthase