Development and validation of an ultra-high-performance liquid chromatography coupled to time-of-flight mass spectrometry method to quantify benzoic acid and long-chain monocarboxylic acids (C12-C28) in atmospheric aerosols.
An ultra-high-performance liquid chromatography/time-of-flight mass spectrometry (with negative ion electrospray ionization) methodology was developed for the simultaneous quantification of benzoic acid and 15 long-chain monocarboxylic acids (MCAs) in ambient aerosols. A fast and quantitative pressurized fluid extraction procedure was optimized using experimental design and the extracts were analyzed without any further clean-up step. Chromatographic separation was achieved on a BEH-C18 column with a mobile phase consisting of 5 mM formic acid in water/acetonitrile (90:10, v/v) and methanol. Excellent precision and accuracies in the MS mass measurements were observed. The method was validated using actual samples spiked with a solution containing either standards or 13C-surrogates. Matrix effects were observed for the C14 MCA only. It was then applied to air particulate reference materials and atmospheric samples collected by a low-volume sampler.
Mirivel
Giovanni
G
Univ. Lille Nord de France, F-59500 Lille, France.
Riffault
Véronique
V
Galloo
Jean-Claude
JC
D000336:Aerosols / Q000737:chemistry; D000393:Air Pollutants / Q000032:analysis*; D053000:Analytic Sample Preparation Methods; D019817:Benzoic Acid / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis*; D005602:France; D008832:Microchemistry / Q000379:methods; D008851:Micropore Filters; D011791:Quartz; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization
D000336:Aerosols; D000393:Air Pollutants; D005227:Fatty Acids; D012997:Solvents; D011791:Quartz; D019817:Benzoic Acid