title: Addressing the malaria drug resistance challenge using flow cytometry to discover new antimalarials.
abstract: A new flow cytometry method that uses an optimized DNA and RNA staining strategy to monitor the growth and development of the Plasmodium falciparum strain W2mef has been used in a pilot study and has identified Bay 43-9006 1, SU 11274 2, and TMC 125 5 as compounds that exhibit potent (<1 microM) overall and ring stage in vitro antimalarial activity.
authors: Center for Global Health & Diseases, Case Western Reserve University, Cleveland, OH 44106-7286, USA.
authors: Brian T
authors: BT
authors: Grimberg
authors: Maria M
authors: MM
authors: Jaworska
authors: Lindsay B
authors: LB
authors: Hough
authors: Peter A
authors: PA
authors: Zimmerman
authors: James G
authors: JG
authors: Phillips
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000032:analysis*; D055808:Drug Discovery; D004351:Drug Resistance; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry / Q000191:economics / Q000379:methods*; D016778:Malaria, Falciparum / Q000188:drug therapy*; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development; D016053:RNA, Protozoan / Q000032:analysis*; D013329:Structure-Activity Relationship
chemical_list: D000962:Antimalarials; D016054:DNA, Protozoan; D016053:RNA, Protozoan