title: Immunohistochemical analysis of ocular platelet basic protein expression during infection with Pseudomonas aeruginosa.
abstract: Platelet basic protein (PBP) and several of its derivatives are known to express a wide range of biological characteristics. It is the precursor of connective tissue activating peptide (CTAP-III), beta thromboglobulin (beta-TG) and neutrophil activating peptide (NAP-2), which is the proteolytic derived end product. The temporal ocular expression of the chemokine PBP before and during corneal infection over several days by Pseudomonas aeruginosa was examined by immunohistochemistry. Prior to corneal infection, immunohistochemical staining demonstrated the constitutive expression of PBP in the cornea, lens and retina. PBP expression was present in the corneal epithelium, stromal fibroblasts and endothelium. There was a temporal increase in PBP expression in the cornea after infection. The entire cornea exhibited extensive cellular infiltration by positive PBP staining infiltrating cells within 6 days post-infection. The cornea, lens and retina underwent extensive degradation within 5-6 days post-infection with some apparent selective increase in PBP staining in the lens and retina.
authors: Richard S
authors: RS
authors: Berk
authors: William J
authors: WJ
authors: Crossland
authors: Mary Ann
authors: MA
authors: Kosir
authors: Minghuan
authors: M
authors: Yu
authors: Yuying
authors: Y
authors: Wang
authors: Sallie
authors: S
authors: Alousi
authors: James
authors: J
authors: Hatfield
authors: Zhong
authors: Z
authors: Dong
mesh_terms: D000818:Animals; D019743:Chemokines, CXC / Q000378:metabolism*; D005123:Eye / Q000378:metabolism*; D015818:Eye Infections, Bacterial / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism*; D007634:Keratitis / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011552:Pseudomonas Infections / Q000378:metabolism*
chemical_list: D019743:Chemokines, CXC; C446301:Cxcl7 protein, mouse; D005136:Eye Proteins