Establishment of an indicator cell line for monitoring bovine immunodeficiency virus infection and inhibitor susceptibility.
Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BIV long terminal repeat was constructed. The BIV indicator cell line, designated BIVE, can detect BIV infection more easily and effectively than the established method, which involves the observation of cell cytopathic effects. Furthermore, viral titration using an assay based on the indicator cells is 100 times more sensitive than the assay based on cytopathic effect. The finding that BIV can infect the hamster cell line expands the known host range of BIV in vitro. The BIV indicator cell line could also be used for the evaluation of the inhibitory effect of antiviral agents. The fusion inhibition effect of the heptad repeat 2 region of the BIV envelope protein could also be quantified.
Yao
Xue
X
Key Laboratory of Molecular Microbiology and Biotechnology, Ministry of Education, Tianjin, China.
Su
Yang
Y
Liu
Chang
C
Tan
Juan
J
Liu
Li
L
Geng
Yun-Qi
YQ
Qiao
Wen-Tao
WT
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics; D016087:Immunodeficiency Virus, Bovine / Q000187:drug effects / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity
D000998:Antiviral Agents; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins