title: [Effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro].
abstract: OBJECTIVE
  To investigate the effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro.
  
  
  METHODS
  Human epidermal squamous cell carcinoma cell line A431 was cultured. After the treatment with triptolide, the inhibition of cellular growth was determined by measuring MTT dye absorption of the living cells. Light microscope showed morphological changes. The cell cycle and apoptosis rate were assessed by flow cytometry.
  
  
  RESULTS
  Triptolide could significantly inhibit the proliferation of A431 cells in a dose- and time-dependent manner. Triptolide could also cause cell morphological changes (the number of floating cells and nuclear pyknosis increase), induce cell apoptosis, and change the distribution of cell cycle phase in A431 cells. Compared with the control group, the G( 0) /G( 1) phase A431 cell rate increased and the rate of S phase cell decreased in TP-treated group. Cell cycles were obviously inhibited by triptolide in G( 0) /G( 1) phase ( both P<0.05).
  
  
  CONCLUSIONS
  TP could play an anti-tumor role by effectively inducing cell apoptosis and inhibiting the proliferation of A431 cells.
authors: Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors: Mingliang
authors: M
authors: Chen
authors: Shuai
authors: S
authors: Tan
authors: Guiying
authors: G
authors: Zhang
authors: Mei
authors: M
authors: Yi
authors: Dan
authors: D
authors: Jian
authors: Hongfu
authors: H
authors: Xie
authors: Xiang
authors: X
authors: Chen
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D010616:Phenanthrenes / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000473:pathology*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide