Generation of Lrp6 conditional gene-targeting mouse line for modeling and dissecting multiple birth defects/congenital anomalies.
Lrp6 is a key coreceptor in the canonical Wnt pathway that is widely involved in tissue/organ morphogenesis. We generated a loxP-floxed Lrp6 mouse line. Crossing with a general Cre deleter, we obtained the Lrp6-floxdel mice, in which the loxP-floxed exon 2 of Lrp6 gene has been deleted ubiquitously. The homozygotes of Lrp6-floxdel mice reproduced typical defects as seen in the conventional Lrp6-deficient mice, such as defects in eye, limb, and neural tube, and die around birth. We also found new phenotypes including cleft palate and agenesis of external genitalia in the Lrp6-floxdel mice. In addition, the Lrp6-deficient embryos are known to be defective in other systems and internal organs including the heart and brain. Thus, by selectively crossing with a lineage-specific or inducible Cre mouse line, the Lrp6 conditional gene-targeting mice will allow us to model specific types of birth defects for mechanism and prevention studies.
Zhou
Chengji J
CJ
Department of Cell Biology and Human Anatomy, University of California, Davis, School of Medicine; Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children-Northern California, Sacramento, California 95817, USA. cjzhou@ucdavis.edu
Wang
Ya-Zhou
YZ
Yamagami
Takashi
T
Zhao
Tianyu
T
Song
Lanying
L
Wang
Kai
K
D000818:Animals; D015152:Blotting, Northern; D000013:Congenital Abnormalities / Q000235:genetics*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D004195:Disease Models, Animal; D018274:Electroporation; D005091:Exons / Q000235:genetics; D018390:Gene Targeting; D015151:Immunoblotting; D026502:LDL-Receptor Related Proteins / Q000172:deficiency* / Q000235:genetics; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D051379:Mice; D008845:Microinjections; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D026502:LDL-Receptor Related Proteins; C555475:LRP6 protein, human; D060488:Low Density Lipoprotein Receptor-Related Protein-6; C555476:Lrp6 protein, mouse