Aedes aegypti densonucleosis virus amplifies, spreads, and reduces host populations in laboratory cage studies.
Aedes densonucleosis virus (family Parcoviridae, genus Brevidensovirus, AeDNV) is a mosquito pathogen that increases Aedes aegypti larval mortality and reduces adult life span. We conducted three laboratory population cage trials, each lasting 16-25 wk. We tested two broad hypotheses. First, Ae. aegypti raised in containers seeded with 10(8) AeDNV genome equivalents/ml (geq/ ml), a concentration feasible for field application, increase AeDNV to concentrations that cause significant adult and larval mortality. Second, infected female mosquitoes disperse AeDNV to uninfected larval habitats. In hypothesis 1, we addressed the rate at which infected larvae secrete virus, how AeDNV titers change in seeded containers over time, whether AeDNV decays over time, and whether AeDNV exposed populations are reduced. In hypothesis 2, we monitored AeDNV concentrations in novel containers after oviposition by infected females. Both hypotheses were supported. Larvae increased AeDNV, secreting virus at a rate of 2.14 x 10(6) geq/larva/d when exposed to 10(8) geq/ml. AeDNV titers reached an asymptote of 10(10) geq/ml by week 10 in seeded containers. AeDNV decayed by 1 log every 4 d as indicated by a reduction in larval mortality. Adult population size was reduced in treated populations. Infected females dispersed AeDNV to novel containers, with titers reaching 10(8) geq/ml. The parameters were used in a Leslie-Lewis matrix model. This model predicted that AeDNV negatively affects Ae. aegypti densities and population structure and thus vectorial capacity.
Suchman
Erica L
EL
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA. Erica.suchman@colostate.edu
Piper
Joseph
J
De Valdez
Megan Wise
MW
Kleker
Brian
B
Neeper
Lenden
L
Plake
Emily
E
Black
William C
WC
Carlson
Jonathan
J
D000330:Aedes / Q000254:growth & development / Q000821:virology*; D000818:Animals; D019223:Densovirinae / Q000502:physiology*; D005260:Female; D007814:Larva / Q000821:virology; D008954:Models, Biological; D009032:Mosquito Control / Q000379:methods; D011156:Population Density; D013997:Time Factors; D014779:Virus Replication