Isoptericola jiangsuensis sp. nov., a chitin-degrading bacterium.
A Gram-positive-staining, non-motile, rod- or coccoid-shaped bacterium, strain CLG(T), was isolated from beach sand from Lianyungang, Jiangsu Province, PR China. The organism grew optimally at 30 degrees C and pH 7.0. Strain CLG(T) contained L-Lys-D-Asp as the peptidoglycan type and galactose, rhamnose and xylose as the whole-cell sugars. It contained anteiso-C(15 : 0) and iso-C(15 : 0) as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphatidylinositol, three phosphoglycolipids and one unidentified lipid. The DNA G+C content was 70.3 mol%. The 16S rRNA gene sequence similarity of strain CLG(T) with Isoptericola dokdonensis DS-3(T) was 99.4 % but the DNA-DNA relatedness between strain CLG( T) and I. dokdonensis DS-3(T) was 58.7 %. There were also many phenotypic properties that distinguished strain CLG( T) from recognized species of the genus Isoptericola. The results showed that strain CLG(T) represents a novel species of the genus Isoptericola, for which the name Isoptericola jiangsuensis sp. nov. is proposed. The type strain is CLG(T) (=DSM 21863( T)=CCTCC AB208287(T)).
Wu
Ying
Y
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of Ministry of Education, and Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Tian
Wei
W
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Zhang
Li-Ping
LP
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Xu
Lian
L
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Shen
Qi-Rong
QR
College of Natural Resource and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China.
Shen
Biao
B
College of Natural Resource and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001493:Bathing Beaches; D002681:China; D002686:Chitin / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D012988:Soil Microbiology; D013045:Species Specificity
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D002686:Chitin; D012822:Silicon Dioxide