title: Differential gene expression signatures of adult peripheral blood vs cord blood monocyte-derived immature and mature dendritic cells.
abstract: OBJECTIVE
  Our previous studies have demonstrated differentially expressed genetic signature patterns in adult peripheral blood (APB) monocytes (Mos) vs cord blood (CB) Mos.
  
  
  METHODS
  In this study, we examined the differential gene expression profiles of APB vs CB immature dendritic cells (iDCs) and mature dendritic cells (mDCs) derived from Mos.
  
  
  RESULTS
  By utilizing oligonucleotide microarray, significant differential gene expression signature patterns were identified in APB vs CB mDCs, including increased expression of chemokines, cytokine receptors, and cell surface molecules. Additionally, signaling protein genes were significantly overexpressed in APB vs CB mDCs. There was also a significant amplification of expression of transcription factor interferon (IFN) regulatory factors and structure regulatory genes in APB vs CB mDCs. In contrast, there were genes expressed significantly higher in CB vs APB mDCs, including cell-cycle regulators and signaling molecule gene.
  
  
  CONCLUSIONS
  Taken together, these results suggest that specific genetic signatures might be responsible for differential DC differentiation and maturation between APB vs CB, and may provide insight into molecular mechanisms regulating differential immune responses between neonates and adults.
authors: Department of Pediatrics, Morgan Stanley Children's Hospital New York Presbyterian, Columbia University, New York, NY 10032, USA.
authors: Hong
authors: H
authors: Jiang
authors: Carmella
authors: C
authors: van de Ven
authors: Laxmi
authors: L
authors: Baxi
authors: Prakash
authors: P
authors: Satwani
authors: Mitchell S
authors: MS
authors: Cairo
mesh_terms: D000328:Adult; D017951:Antigen Presentation; D001773:Blood Cells / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D009000:Monocytes / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D018925:Chemokines; D047908:Intracellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D018121:Receptors, Cytokine; D007371:Interferon-gamma