title: Arylacetamide deacetylase attenuates fatty-acid-induced triacylglycerol accumulation in rat hepatoma cells.
abstract: Mobilization of hepatic triacylglycerol stores provides substrates for mitochondrial beta-oxidation and assembly of VLDLs; however, the identity of lipolytic enzymes involved in the regulation of this process remains largely unknown. Arylacetamide deacetylase (AADA) shares homology with hormone-sensitive lipase and therefore could potentially participate in hepatic lipid metabolism, including the regulation of hepatic triacylglycerol levels. We have established McArdle-RH7777 (rat hepatoma) cell lines stably expressing mouse AADA cDNA and performed metabolic labeling as well as lipid mass analyses. Expression of AADA cDNA in McArdle-RH7777 cells significantly reduced intracellular triacylglycerol levels and apolipoprotein B secretion and increased fatty acid oxidation. These results suggest that fatty acids released by AADA-mediated hydrolysis of lipids are channeled for -oxidation rather than for the assembly of lipoproteins.
authors: Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
authors: Vivien
authors: V
authors: Lo
authors: Bruce
authors: B
authors: Erickson
authors: Michaela
authors: M
authors: Thomason-Hughes
authors: Kerry W S
authors: KW
authors: Ko
authors: Vernon W
authors: VW
authors: Dolinsky
authors: Randy
authors: R
authors: Nelson
authors: Richard
authors: R
authors: Lehner
mesh_terms: D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005227:Fatty Acids / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D021381:Protein Transport; D051381:Rats; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D014280:Triglycerides; C439668:Aadac protein, mouse; D002265:Carboxylic Ester Hydrolases; D008049:Lipase