title: DNA damage, repair and photoadaptation in a Xiphophorus fish hybrid.
abstract: Exposure to sunlight is responsible for most cutaneous malignant melanomas in the human population. It is very likely that DNA damage is an initial event in melanomagenesis, however, the role played by this damage is an open question. To this end, we used a hemipigmented F(1) hybrid of the fish genus Xiphophorus and HPLC tandem mass spectrometry to examine the effects of melanin on the induction and repair of the predominant UV-induced photoproducts formed in skin cell DNA. We found that heavily pigmented skin cells had about half the damage of nonpigmented cells and the relative induction of the major photoproducts was independent of the degree of pigmentation. The efficiency of photoenzymatic repair was the same in nonpigmented and pigmented areas of the fish. We found no evidence of residual damage at 10 days after the last exposure. Most striking was that repeated exposure to multiple doses of UVB caused a very significant photoadaptive response. Rather than an accumulation of damage after five doses of UVB we saw a significant reduction in the amount of damage induced after the final dose compared with the initial dose. The relevance of these observations is discussed in the context of melanoma susceptibility and UVB thresholds.
authors: Department of Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX, USA. dmitchell@mdanderson.org
authors: David L
authors: DL
authors: Mitchell
authors: Lakshmi
authors: L
authors: Paniker
authors: Thierry
authors: T
authors: Douki
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D002678:Chimera; D003532:Cyprinodontiformes / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair; D019281:Dimerization; D004195:Disease Models, Animal; D006801:Humans; D008543:Melanins / Q000528:radiation effects*; D011743:Pyrimidines / Q000737:chemistry; D013472:Sunlight; D014466:Ultraviolet Rays
chemical_list: D008543:Melanins; D011743:Pyrimidines; C030986:pyrimidine