title: Changes in the gene expression of the white rot fungus Phanerochaete chrysosporium due to the addition of atropine.
abstract: We constructed a LongSAGE (Long Serial Analysis of Gene Expression) library from a 3-d culture of Phanerochaete chrysosporium supplemented with atropine, which inhibits the production of lignin-degrading enzymes. The library (the atropine library) contains 13,108 LongSAGE tags and 6,783 unique tags. The gene expression profile represented by the tags was compared with those of two previously constructed libraries, one of which was constructed using 2-d cultures in which the fungus had not yet produced ligninolytic enzymes (the 2-d library) and the other was constructed using 3-d cultures in which the fungus had just started to produce the enzymes (the 3-d library). We found a total of 595 genes that were at least twice more highly or at least twice less highly expressed in the 3-d library than in the 2-d library or the atropine library, and the fluctuations were statistically significant. The relationships among these 595 genes were considered using cluster analysis. Of the 595 genes, 164 showed expression patterns similar to those of four ligninolytic enzyme genes, which were more expressed on day 3 than under any other conditions. Many of these 164 genes comprised genes possibly involved in lignin degradation, lipid metabolism, xenobiotic degradation, stress response, or signal transduction pathways.
authors: School of Environmental Science, The University of Shiga Prefecture, Hikone, Shiga, Japan.
authors: Masahiko
authors: M
authors: Minami
authors: Kazumi
authors: K
authors: Suzuki
authors: Akifumi
authors: A
authors: Shimizu
authors: Tomohiro
authors: T
authors: Hongo
authors: Takaiku
authors: T
authors: Sakamoto
authors: Naoki
authors: N
authors: Ohyama
authors: Hironori
authors: H
authors: Kitaura
authors: Akiho
authors: A
authors: Kusaka
authors: Kenji
authors: K
authors: Iwama
authors: Toshikazu
authors: T
authors: Irie
mesh_terms: D001285:Atropine / Q000494:pharmacology*; D001483:Base Sequence; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D015723:Gene Library; D005800:Genes, Fungal; D008031:Lignin / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D020075:Phanerochaete / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D003470:Culture Media; D001285:Atropine; D008031:Lignin