title: Genome wide expression analysis of the effect of Pinelliae Rhizoma extract on psychological stress.
abstract: Pinelliae Rhizoma has been used traditionally as an antidepressant in Oriental medicine. In this study, the effect of Pinelliae Rhizoma extract (PRe) on psychological stress was investigated in mice. The results of an elevated plus-maze experiment revealed that application of psychological stress to mice led to the development of an abnormal behavioral pattern. However, oral administration of PRe significantly reduced the abnormal behavior of mice with a recovery rate of 75.5%. To elucidate the molecular mechanism by PRe, a microarray analysis of the brains of mice was conducted. The results of this analysis revealed that 456 genes were up-regulated and 392 genes were down-regulated in response to psychological stress. The expression of most of the genes that were altered in response to psychological stress was restored to normal levels in PRe treated mice, with a recovery rate of 81.5% and 85.2% being observed for up- and down-regulated genes, respectively. Finally, when the interaction network information was analysed, the recovery rate of the core node genes (46 up- and 29 down-regulated genes) in PRe treated mice was found to be over 95%, which indicates that this final set of genes may be the effective target of PRe.
authors: Department of Constitutional Medicine Research, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejon 305-701, Republic of Korea.
authors: Bu-Yeo
authors: BY
authors: Kim
authors: Su Jin
authors: SJ
authors: Cho
authors: Hyung-Woo
authors: HW
authors: Kim
authors: Su-Youn
authors: SY
authors: Kim
authors: Se-Hyun
authors: SH
authors: Lim
authors: Kyeong-Ok
authors: KO
authors: Kim
authors: Chang-Won
authors: CW
authors: Choi
authors: Chi-Yeon
authors: CY
authors: Lim
authors: Boo-Hyeong
authors: BH
authors: Byun
authors: Su-In
authors: SI
authors: Cho
authors: Guem-San
authors: GS
authors: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D008517:Phytotherapy; D030021:Pinellia / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D013315:Stress, Psychological / Q000188:drug therapy*
chemical_list: D010936:Plant Extracts