Surface modification of a titanium alloy with a phospholipid polymer prepared by a plasma-induced grafting technique to improve surface thromboresistance.
To improve the thromboresistance of a titanium alloy (TiAl(6)V(4)) surface which is currently utilized in several ventricular assist devices (VADs), a plasma-induced graft polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) was carried out and poly(MPC) (PMPC) chains were covalently attached onto a TiAl(6)V(4) surface by a plasma induced technique. Cleaned TiAl(6)V(4) surfaces were pretreated with H(2)O-vapor-plasma and silanated with 3-methacryloylpropyltrimethoxysilane (MPS). Next, a plasma-induced graft polymerization with MPC was performed after the surfaces were pretreated with Ar plasma. Surface compositions were verified by X-ray photoelectron spectroscopy (XPS). In vitro blood biocompatibility was evaluated by contacting the modified surfaces with ovine blood under continuous mixing. Bulk phase platelet activation was quantified by flow cytometric analysis, and surfaces were observed with scanning electron microscopy after blood contact. XPS data demonstrated successful modification of the TiAl(6)V(4) surfaces with PMPC as evidenced by increased N and P on modified surfaces. Platelet deposition was markedly reduced on the PMPC grafted surfaces and platelet activation in blood that contacted the PMPC-grafted samples was significantly reduced relative to the unmodified TiAl(6)V(4) and polystyrene control surfaces. Durability studies under continuously mixed water suggested no change in surface modification over a 1-month period. This modification strategy shows promise for further investigation as a means to reduce the thromboembolic risk associated with the metallic blood-contacting surfaces of VADs and other cardiovascular devices under development.
Ye
Sang Ho
SH
McGowan Institute for Regenerative Medicine, University of Pittsburgh, 100 Technology Dr., Suite 200, Pittsburgh, PA 15219, USA.
Johnson
Carl A
CA
Woolley
Joshua R
JR
Oh
Heung-Il
HI
Gamble
Lara J
LJ
Ishihara
Kazuhiko
K
Wagner
William R
WR
D000497:Alloys / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000378:metabolism; D006041:Goats; D008422:Materials Testing / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D015539:Platelet Activation; D011109:Polymethacrylic Acids; D013057:Spectrum Analysis; D013500:Surface Tension; D013927:Thrombosis / Q000503:physiopathology / Q000517:prevention & control*; D014025:Titanium / Q000737:chemistry*
D000497:Alloys; D001672:Biocompatible Materials; D008689:Methacrylates; D011109:Polymethacrylic Acids; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; D014025:Titanium