title: Pulsatile shear stress increased mitochondrial membrane potential: implication of Mn-SOD.
abstract: Mitochondrial dysfunction is intimately involved in cardiovascular diseases. Mitochondrial membrane potential (DeltaPsi(m)) is coupled with oxidative phosphorylation to drive ATP synthesis. In this study, we examined the effect of physiological pulsatile shear stress (PSS) on DeltaPsi(m) and the role of Mn-SOD expression on DeltaPsi(m). Confluent human aortic endothelial cells (HAEC) were exposed to PSS, and DeltaPsi(m) was monitored using tetramethylrhodamine methyl ester (TMRM(+)), a mitochondrial membrane potential probe. PSS significantly increased DeltaPsi(m) and the change in DeltaPsi(m) was a dynamic process. DeltaPsi(m) returned to baseline level after PSS for 2h followed by static state for 4h. Mitochondrial Mn-SOD expression and activities were also significantly up-regulated in response to PSS. Silencing Mn-SOD attenuated PSS-mediated DeltaPsi(m) increase while adding Mn-SOD mimetic, MnTMPyP, increased DeltaPsi(m) to the similar extent as induced by PSS. Our findings suggest that PSS-increased mitochondrial DeltaPsi(m), in part, via Mn-SOD up-regulation.
authors: Department of Biomedical Engineering and Division of Cardiovascular Medicine, University of Southern California, Los Angeles, CA, USA.
authors: Rongsong
authors: R
authors: Li
authors: Tyler
authors: T
authors: Beebe
authors: Jeffrey
authors: J
authors: Cui
authors: Mahsa
authors: M
authors: Rouhanizadeh
authors: Lisong
authors: L
authors: Ai
authors: Pin
authors: P
authors: Wang
authors: Martin
authors: M
authors: Gundersen
authors: Wakako
authors: W
authors: Takabe
authors: Tzung K
authors: TK
authors: Hsiai
mesh_terms: D001011:Aorta / Q000201:enzymology / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011673:Pulsatile Flow / Q000187:drug effects; D012235:Rhodamines / Q000494:pharmacology; D033081:Shear Strength; D013314:Stress, Mechanical; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D012235:Rhodamines; C401833:tetramethylrhodamine methyl ester; D013482:Superoxide Dismutase