Screening and identification of unexpected red cell antibodies by simultaneous LISS/Coombs and NaCl/Enzyme gel methods.
We evaluated the clinical usefulness of simultaneous LISS/Coombs and NaCl/Enzyme testing using the gel method for screening and identification of unexpected antibodies in 15,014 samples. When unexpected antibodies were detected by either screening test, those antibodies were identified using both the LISS/Coombs and the NaCl/Enzyme gel test. The positive screening rates of the LISS/Coombs, NaCl/Enzyme, and combined tests (excluding 25 autoantibody cases) were 0.48%, 1.29%, and 1.39%, respectively. Among the 57 samples positive by both screening methods, the antibodies in 19.3% could be identified only by the NaCl/Enzyme method. Among the 137 samples positive only by NaCl/Enzyme screening, 74.5% showed positive results in antibody identification only by the NaCl/Enzyme test, although 7.3% were also positive in the LISS/Coombs test. The NaCl/Enzyme method thus showed about threefold higher detection rates than the LISS/Coombs method, especially in screening for Rh antibodies, and higher exact identification rates and discriminatory power for identifying mixed antibodies. Addition of the NaCl/Enzyme method to routine laboratory procedures may detect and identify considerable numbers of significant antibodies that might be missed if only the LISS/Coombs method is used.
Shin
Jeong Hwan
JH
Department of Laboratory Medicine, College of Medicine, Inje University, Busan, Korea.
Lee
Ja Young
JY
Kim
Jae Hyen
JH
Kim
Hye Ran
HR
Lee
Jeong Nyeo
JN
D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D003298:Coombs Test; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis; D011933:Reagent Kits, Diagnostic
D000906:Antibodies; D007518:Isoantibodies; C033242:RH-antibodies; D011933:Reagent Kits, Diagnostic