Purification and characterization of mycoferritin  from Fusarium verticillioides MRC 826.
The fungus Fusarium verticillioides MRC 826 (ascomycetes species), a toxigenic isolate is capable of synthesizing mycoferritin only upon induction with iron in yeast extract sucrose medium. The molecular mass, yield, iron and carbohydrate contents of the purified mycoferritin were 460 kDa, 0.010 mg/g of wet mycelia, 1.0 and 40.2%, respectively. Native gel electrophoresis of the mycoferritin revealed two bands possibly representing isoforms of ferritin. Subunit analysis by SDS-PAGE showed a single protein subunit of ~24 kDa suggesting similar sized subunits in the structure of apoferritin shell. Immunological cross reactivity was observed with the anti-fish liver ferritin. Transmission electron microscopy revealed an apparent particle size of 100 Å. N-terminal amino acid sequencing of mycoferritin showed identities with other eukaryotic ferritin sequences. The spectral characteristics were similar to equine spleen ferritin. However, circular dichroic spectra revealed a higher degree of helicity. Functionally, induction of mycoferritin minimizes the prooxidant role of iron.
Validandi
Vakdevi
V
Department of Biochemistry, University College of Science, Osmania University, Hyderabad, Andhra Pradesh, India.
Rupula
Karuna
K
Beedu
Sashidhar Rao
SR
Deshpande
Vijay
V
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005399:Fishes; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005670:Fusarium / Q000737:chemistry; D006736:Horses; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010316:Particle Size; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013154:Spleen / Q000737:chemistry
D000975:Antioxidants; D005656:Fungal Proteins; D020033:Protein Isoforms; D021122:Protein Subunits; D005293:Ferritins; D007501:Iron