Fed-batch culture of astaxanthin-rich Haematococcus pluvialis by exponential nutrient feeding and stepwise light supplementation.
A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO(2)-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 micromol photon/m(2)/s, while keeping the other conditions in the CO(2)-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.
Kang
Chang Duk
CD
Nano Optics and Biomolecular Engineering National Laboratory, Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea.
Han
Se Jong
SJ
Choi
Seung Phill
SP
Sim
Sang Jun
SJ
D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods; D000460:Chlorophyta / Q000166:cytology / Q000254:growth & development*; D008027:Light; D024341:Xanthophylls / Q000096:biosynthesis / Q000302:isolation & purification
D024341:Xanthophylls; C005948:astaxanthine