Murdochiella asaccharolytica gen. nov., sp. nov., a Gram-stain-positive, anaerobic coccus isolated from human wound specimens.
Two strains of previously unknown Gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, WAL 1855C(T) and WAL 2038E, are genotypically homogeneous and constitute a novel lineage within Clostridium cluster XIII. There was 13-14 % 16S rRNA gene sequence divergence between the novel strains and the most closely related species, Parvimonas micra, Finegoldia magna and species of Helcococcus. Based on the phenotypic and phylogenetic findings, a novel genus and species, Murdochiella asaccharolytica gen. nov., sp. nov., are proposed. Strain WAL 1855C(T) (=ATCC BAA-1631(T) =CCUG 55976(T)) is the type strain of Murdochiella asaccharolytica.
Ulger-Toprak
Nurver
N
Marmara University, Faculty of Medicine, Department of Microbiology, Istanbul, Turkey.|Research Service, VA Medical Center West Los Angeles, Los Angeles, CA 90073, USA.
Liu
Chengxu
C
Research Service, VA Medical Center West Los Angeles, Los Angeles, CA 90073, USA.
Summanen
Paula H
PH
Research Service, VA Medical Center West Los Angeles, Los Angeles, CA 90073, USA.
Finegold
Sydney M
SM
Departments of Medicine and of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA, USA.|Infectious Diseases Section, VA Medical Center West Los Angeles, Los Angeles, CA 90073, USA.
D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006095:Gram-Positive Cocci / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014946:Wound Infection / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S