In silico analysis of the structure of variable domains of mouse single-chain antibodies specific to the human recombinant interferon beta1b.
In silico analysis of the DNA encoding single-chain Fv antibodies (ScFv) specific to the human recombinant interferon beta1b and alpha2b (rhIFN-beta1b, rhIFN-alpha2b) has been carried out. The V-, D- and J-gene segments, the complementarity-determining (CDR) and framework (FR) regions, n-nucleotides as well as mutation rates which take place during the affinity maturation of the examined sequences have been determined. For the panel of ScFv against rhIFN-alpha1b isolated from an immune combinatorial cDNA library uniqueness of the CDRH3 loop by the length and amino acid composition has been shown. Multiple alignments with the nearest homologies from the NCBI databases have revealed that the sequences of ScFv obtained are new.
Flyak
A I
AI
National Taras Shevchenko University of Kyiv, Ukraine. flyaka@mail.ru
Pavlova
M V
MV
Gilchuk
P V
PV
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D057127:Single-Chain Antibodies; C055966:immunoglobulin Fv; D000068576:Interferon beta-1b; D016899:Interferon-beta