title: Improved isolation methods for mucosal leukocytes from small and large intestines in rats.
abstract: We optimized the isolation protocol for intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) from the rat small intestine, and LPLs from even the rat large intestine. The major population of IELs in the small intestine was considered to be from the villus epithelia. The cytotoxicity of mucosal leukocytes was comparable among isolated fractions from both the small and large intestines, regardless of the population differences. Further analyses of the cells collected from other lymphoid tissues demonstrated that CD161(+) cells selectively accumulated in the intestinal lamina propria and did not recirculate through the lymph ducts. Our modified isolation protocol enables the collection of mucosal immune cells from the rat intestines without any deterioration of cell function and could contribute to a better understanding of dietary influences on the mucosal immune system.
authors: Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors: Jae-Sung
authors: JS
authors: Lee
authors: Kohsuke
authors: K
authors: Oka
authors: Mie
authors: M
authors: Obara
authors: Megumi
authors: M
authors: Nishimukai
authors: Yung-Choon
authors: YC
authors: Yoo
authors: Kaori
authors: K
authors: Yamada
authors: Takamitsu
authors: T
authors: Tsukahara
authors: Keizo
authors: K
authors: Nakayama
authors: Hiroshi
authors: H
authors: Hara
authors: Satoshi
authors: S
authors: Ishizuka
mesh_terms: D000818:Animals; D002432:Cecum / Q000166:cytology; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D003106:Colon / Q000166:cytology; D007413:Intestinal Mucosa / Q000166:cytology*; D007420:Intestine, Large / Q000166:cytology*; D007421:Intestine, Small / Q000166:cytology*; D007962:Leukocytes / Q000166:cytology*; D051379:Mice; D051381:Rats; D015203:Reproducibility of Results