title: Fibrocollagen-covered prosthesis for a noncircumferential segmental tracheal replacement.
abstract: OBJECTIVE
  Fibrocollagen-covered polyester meshes can be used as possible substitutions for tracheal segments if they become integrated into the tissue without complications. The aim of this study was to assess a fibrocollagen-covered polyester prosthesis to be used as a substitution for a tracheal segment.
  
  
  METHODS
  We performed a blind, randomized experimental assay. Adult Wistar rats were assigned to one of 2 groups. Prostheses were made by implanting polyester tubing in a group of animals to cover them with homologous collagen. They were implanted as substitutions of tracheal segments in the experimental group after creating a defect in the anterior wall of the trachea. Clinical, histomorphologic, and immunohistochemical assessments were made at 4 different time points.
  
  
  RESULTS
  The experimental group presented some respiratory distress signs during the first 7 to 10 days, such as stertors, hissing, and low motor activity. After this initial period, the symptoms subsided progressively and disappeared at the end of the first month. These respiratory symptoms caused no mortality. Initially undifferentiated monolayer cells predominated on the implant's surface, but during the last 2 months, the proportion of epithelial and ciliated cells was similar to that seen in control animals. Types I, III, and V collagen fibers were identified around the mesh. The intraluminal area of the tracheas with prostheses and prosthesis thickness were larger during the 4 months of the experiment. The increase in thickness was due to angiogenesis without evidence of fibrosis or chronic inflammation.
  
  
  CONCLUSIONS
  Polyester-collagen prostheses used as substitutions of tracheal segments in rats enabled the proliferation of normal respiratory epithelium and maintained tracheal function without collapse, inflammatory reaction, or secondary stenosis.
authors: Experimental Surgery, Experimental Medicine Division, Instituto Nacional de Pediatría, Mexico. fvadiaz@prodigy.net.mx
authors: Fernando
authors: F
authors: Villegas-Alvarez
authors: José F
authors: JF
authors: González-Zamora
authors: Angelica
authors: A
authors: González-Maciel
authors: Rosa
authors: R
authors: Soriano-Rosales
authors: Beatriz
authors: B
authors: Pérez-Guille
authors: Luis
authors: L
authors: Padilla-Sánchez
authors: Rafael
authors: R
authors: Reynoso-Robles
authors: Andrea
authors: A
authors: Ramos-Morales
authors: Edgar
authors: E
authors: Zenteno-Galindo
authors: Armando
authors: A
authors: Pérez-Torres
authors: Eduardo E
authors: EE
authors: Montalvo-Jave
mesh_terms: D000818:Animals; D003094:Collagen; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D011091:Polyesters; D011474:Prosthesis Design; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea; D016896:Treatment Outcome
chemical_list: D011091:Polyesters; D003094:Collagen