title: Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population.
abstract: BACKGROUND
  Nonspecific lipid transfer proteins (LTPs) represent potent pollen and food allergens. However, the allergenic properties of peanut LTP have not been studied.
  
  
  OBJECTIVE
  To identify LTP in peanut extract using sera from subjects with peanut allergy and Pru p 3-sensitized subjects from Southern Europe, clone and express this protein, and obtain information on the importance as allergen for these selected patients.
  
  
  METHODS
  Peanut LTP (Ara h 9) was cloned and sequenced by using a combination of bioinformatic and molecular biology tools (PCR, immunoblotting, Basic Local Alignment Search Tool [BLAST] searches). The immunologic properties of Ara h 9, Ara h 1, Ara h 2, and Ara h 3 were studied by using sera from subjects with peanut and peach allergy from Italy by immunoblotting and allergen microarray technology.
  
  
  RESULTS
  Two Ara h 9 isoforms-Ara h 9.01 and Ara h 9.02-were cloned and expressed. Ara h 9 represented a minor allergen for subjects with peanut allergy. However, including Ara h 9 as single component for serologic detection of sensitization to peanut by component-resolved diagnosis seems crucial, because the frequency of sensitization to the classic major peanut allergens Ara h 1, Ara h 2, and Ara h 3 was low in these patients from Southern Europe.
  
  
  CONCLUSIONS
  Ara h 9 is a new member of the LTP allergen family that seems to play an important role in peanut allergy for patients from the Mediterranean area.
authors: Research Center Borstel, Borstel, Germany.
authors: Susanne
authors: S
authors: Krause
authors: Gerald
authors: G
authors: Reese
authors: Stefanie
authors: S
authors: Randow
authors: Danila
authors: D
authors: Zennaro
authors: Donato
authors: D
authors: Quaratino
authors: Paola
authors: P
authors: Palazzo
authors: Maria Antonietta
authors: MA
authors: Ciardiello
authors: Arnd
authors: A
authors: Petersen
authors: Wolf-Meinhard
authors: WM
authors: Becker
authors: Adriano
authors: A
authors: Mari
mesh_terms: D055516:2S Albumins, Plant / Q000276:immunology / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology* / Q000378:metabolism; D052179:Antigens, Plant / Q000276:immunology / Q000378:metabolism; D010367:Arachis / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002648:Child; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D021183:Peanut Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D010940:Plant Proteins / Q000276:immunology* / Q000378:metabolism; D020033:Protein Isoforms / Q000276:immunology / Q000378:metabolism; D055314:Seed Storage Proteins / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; C500890:Ara h 2 allergen, Arachis hypogaea; C544631:Ara h 9 allergen, peanut; D002352:Carrier Proteins; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; D020033:Protein Isoforms; D055314:Seed Storage Proteins; C118207:allergen Ara h3; C030363:lipid transfer protein; D007073:Immunoglobulin E