Effects of protein concentration and detergent on endotoxin reduction by ultrafiltration.
Lipopolysaccharide (LPS), found in the outer membrane of Gram negative bacteria, only exerts its toxic effects when in free form. LPS has three major parts, lipid A, the toxic component, along with a core polysaccharide and O-specific polysaccharide. LPS monomers are known to have molecular masses between 10 to 30 kDa. Under physiological conditions, LPS exists in equilibrium between monomer and vesicle forms. LPS removal by 100 kDa ultrafiltration was more efficient (99.6% of LPS removed) with a low concentration of protein (2.0 mg/ml) compared to a high concentration (20.1 mg/ml). In the presence of different detergents (0.5% Tween 20, 1.0% taurodeoxycholate and 1.0% Triton X-100), LPS removal was more efficient at low protein concentrations (2.0 mg/ml) compared to high protein concentrations (20.1 mg/ml).
Jang
Hyun
H
1R&D Center, Komipharm Co., Shihung, Korea.
Kim
Hyo-Seung
HS
Moon
Seung-Cheol
SC
Lee
Young-Rae
YR
Yu
Kang-Yeoul
KY
Lee
Byeong-Kil
BK
Youn
Hyun Zo
HZ
Jeong
Young-Ju
YJ
Kim
Byeong-Soo
BS
Lee
Sung-Ho
SH
Kim
Jong-Suk
JS
D003902:Detergents / Q000494:pharmacology*; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification*; D007218:Industrial Microbiology / Q000379:methods; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification; D008970:Molecular Weight; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D014462:Ultrafiltration / Q000379:methods; D018508:Water Purification / Q000379:methods
D003902:Detergents; D004731:Endotoxins; D008070:Lipopolysaccharides; D011506:Proteins