Generation of an OMgp allelic series in mice.
The very limited ability to regenerate axons after injury in the mature mammalian central nervous system (CNS) has been partly attributed to the growth restrictive nature of CNS myelin. Oligodendrocyte myelin glycoprotein (OMgp) was identified as a major myelin-derived inhibitor of axon growth. However, its role in axon regeneration in vivo is poorly understood. Here we describe the generation and molecular characterization of an OMgp allelic series. With a single gene targeting event and Cre/FLP mediated recombination, we generated an OMgp null allele with a LacZ reporter, one without a reporter gene, and an OMgp conditional allele. This allelic series will aid in the study of OMgp in adult CNS axon regeneration using mouse models of spinal cord injury. The conditional allele will overcome developmental compensation when employed with an inducible Cre, and allows for the study of temporal and tissue/cell type-specific roles of OMgp in CNS injury-induced axonal plasticity.
Lee
Jae K
JK
Department of Neurosciences, University of California San Diego, School of Medicine, La Jolla, California 92093-0691, USA.
Case
Lauren C
LC
Chan
Andrea F
AF
Zhu
Yuhong
Y
Tessier-Lavigne
Marc
M
Zheng
Binhai
B
D000483:Alleles; D000818:Animals; D001369:Axons; D015153:Blotting, Western; D058851:GPI-Linked Proteins; D018390:Gene Targeting; D006720:Homozygote; D051379:Mice; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000235:genetics*; D063308:Myelin-Oligodendrocyte Glycoprotein; D013119:Spinal Cord Injuries / Q000473:pathology; D013629:Tamoxifen / Q000008:administration & dosage
D058851:GPI-Linked Proteins; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; C560529:Omg protein, mouse; D013629:Tamoxifen