title: Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis.
abstract: Site-directed incorporation of PEGylated nonnatural amino acids with 4, 8, and 12 repeated ethylene glycol units was examined in a cell-free translation system. PEGylated aminophenylalanine derivatives were successfully incorporated into proteins, whereas PEGylated lysines were not. The incorporation efficiency of the PEGylated amino acids decreased with an increase in PEG chain length. The present method will be useful for preparation of proteins which are PEGylated in a site-specific and quantitative manner.
authors: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa, Japan.
authors: Naoki
authors: N
authors: Shozen
authors: Issei
authors: I
authors: Iijima
authors: Takahiro
authors: T
authors: Hohsaka
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics; D000926:Anticodon / Q000235:genetics; D002474:Cell-Free System; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D046188:Protein Modification, Translational; D011506:Proteins / Q000737:chemistry*; D012343:RNA, Transfer / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000926:Anticodon; D011506:Proteins; D011092:Polyethylene Glycols; D012343:RNA, Transfer