title: [Screening study of the kinetogenic effects of serum containing four Chinese materia medicas on the colonic smooth muscle cells in rats].
abstract: OBJECTIVE
  To study the kinetogenic effects of serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala on the colonic smooth muscle cells of rats.
  
  
  METHODS
  Serum containing Chinese materia medicas was made according to standard methods. Smooth muscle cells were isolated from the muscle layers of Wistar rat's colon, referred to modified Bitar's method. The contractile response of colonic smooth muscle cells to serum containing Chinese materia medicas (10%, 50%, 100% concentration) and other medicines (blank and 1 x 10(-3) mol/L acetylcholine) were separately observed. The contractility was presented by the decrease of the cell length between the drug groups and the control.
  
  
  RESULTS
  Serum containing each Chinese materia medica can make dose-dependent contraction at different concentrations (P < 0.05), but the strongest effect of each serum had no significant difference (P > 0.05).
  
  
  CONCLUSIONS
  Serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala can make notable contraction on colonic smooth muscle cells in rats.
authors: The Second Affiliated Hospital of Shandong University, Jinan. jianqiangg@sdu.edu.cn
authors: Yan-Ting
authors: YT
authors: Shi
authors: Chun-Feng
authors: CF
authors: Si
authors: Bin
authors: B
authors: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal