title: Effect of zona incision by piezo-micromanipulator (ZIP) on in vitro fertilization in 21 transgenic mice lines.
abstract: Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is effective for IVF using cryopreserved genetically modified mouse spermatozoa.
authors: Chugai Research Institute for Medical Science, Komakado, Gotemba, Shizuoka, Japan.
authors: Yosuke
authors: Y
authors: Kawase
authors: Takanori
authors: T
authors: Tachibe
authors: Toshio
authors: T
authors: Hani
authors: Hiromi
authors: H
authors: Tateishi
authors: Kou-Ichi
authors: K
authors: Jishage
authors: Hiroshi
authors: H
authors: Suzuki
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005260:Female; D005298:Fertility / Q000502:physiology; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D009865:Oocytes / Q000502:physiology*; D012662:Semen Preservation; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*