Resistance of a recombinant Escherichia coli to dehydration.
Dehydration of microorganisms, rendering them anhydrobiotic, is often an efficient method for the short and long term conservation of different strain-producers. However, some biotechnologically important recombinant bacterial strains are extremely sensitive to conventional treatment. We describe appropriate conditions during dehydration of the recombinant Escherichia coli strain HB 101 (GAPDH) that can result dry cells having a approximately 88% viability on rehydration. The methods entails air-drying after addition of 100 mM trehalose to the cultivation medium or distilled water (for short term incubation).
Khroustalyova
Galina
G
Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, LV-1586 Riga, Latvia.
Boudrant
Joseph
J
Rapoport
Alexander
A
D003890:Desiccation; D004926:Escherichia coli / Q000502:physiology*; D050296:Microbial Viability; D011309:Preservation, Biological; D011995:Recombination, Genetic; D014199:Trehalose
D014199:Trehalose