title: Serum mucosa-associated epithelial chemokine (MEC/CCL28) in atopic dermatitis: a specific marker for severity.
abstract: BACKGROUND
  Mucosa-associated epithelial chemokine (MEC; CCL28) is considered to be pivotal in mediating the migration of CC chemokine receptor 3- and 10- (CCR3- and CCR10)-expressing, skin-homing, memory, cutaneous lymphocyte-associated antigen-positive (CLA(+)) T cells. CCL28 is selectively and continuously expressed by epidermal keratinocytes, but highly upregulated in inflammatory skin diseases, such as atopic dermatitis (AD).
  
  
  OBJECTIVE
  This controlled longitudinal study was designed to evaluate the expression of CCL28 in serum in childhood AD and bronchial asthma (BA), and its possible relationship to disease severity and activity.
  
  
  METHODS
  Serum CCL28 levels were measured in 36 children with AD, 23 with BA, 14 with both conditions, and 21 healthy age- and sex-matched controls. Sixteen patients in the AD group were followed up and resampled for serum CCL28 after clinical remission. Serum CCL28 levels were correlated with some AD disease activity and severity variables.
  
  
  RESULTS
  Serum CCL28 levels in AD, whether during flare [median, 1530 pg/mL; mean +/- standard deviation (SD) = 1590.4 +/- 724.3 pg/mL] or quiescence (median, 1477 pg/mL; mean +/- SD = 1575.2 +/- 522.1 pg/mL), were significantly higher than those in healthy children (median, 301 pg/mL; mean +/- SD = 189.6 +/- 92.8 pg/mL); however, the levels during flare and quiescence were statistically comparable. The serum levels in BA (median, 340 pg/mL; mean +/- SD = 201.6 +/- 109.5 pg/mL) were significantly lower than those in the AD group, and comparable with those in healthy controls. Serum CCL28 levels in severe AD were significantly higher than those in mild and moderate cases, and correlated positively with the calculated severity scores [Leicester Sign Score (LSS) and Scoring Atopic Dermatitis (SCORAD)]. CCL28 levels during the exacerbation of AD were positively correlated with the corresponding values during remission, the peripheral absolute eosinophil counts, and serum lactate dehydrogenase levels. Serum CCL28 levels were not correlated with the serum total immunoglobulin E values in AD.
  
  
  CONCLUSIONS
  Our data reinforce the concept that CCL28 might contribute to the pathogenesis of AD, probably through the selective migration and infiltration of effector/memory T-helper-2 cells in the skin. CCL28 may also represent an objective prognostic marker for disease severity. Further studies may pave the way for CCL28 antagonism among adjuvant therapeutic strategies.
authors: Faculty of Medicine, Ain Shams University, Cairo, Egypt. ezzatmhm@hotmail.com
authors: Mohamed Hesham Mohamed
authors: MH
authors: Ezzat
authors: Mohamed Abdelnaeem
authors: MA
authors: Sallam
authors: Kareem Yehia Ali
authors: KY
authors: Shaheen
mesh_terms: D001249:Asthma / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D002465:Cell Movement / Q000276:immunology; D019742:Chemokines, CC / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology
chemical_list: D015415:Biomarkers; C414047:CCL28 protein, human; D019742:Chemokines, CC