title: Tolllike receptor 4 (TLR4) polymorphisms in Tunisian patients with Crohn's disease: genotype-phenotype correlation.
abstract: BACKGROUND
  The immune responses to bacterial products through the pattern recognition receptor (PRR) play a pivotal role in pathogenesis of Crohn's disease. A recent study described an association between CD and some gene coding for bacterial receptor like NOD2/CARD15 gene and TLR4. In this study, we sought to determine whether TLR4 gene was associated with Crohn's disease (CD) among the Tunisian population and its correlation with clinical manifestation of the disease.
  
  
  METHODS
  90 patients with CD and 80 healthy individuals are genotyped for the Asp299Gly and Thr399Ile polymorphisms by restriction fragment length polymorphism analysis.
  
  
  RESULTS
  The allele and genotype frequency of the TLR4 polymorphisms did not differ between patients and controls. The genotype-phenotype correlation permitted to show that the Thr399Ile polymorphism was associated with early onset disease.
  
  
  CONCLUSIONS
  this study reported the absence of association between CD and TLR4 gene in the Tunisian population, but this gene could play a role in clinical expression of the disease.
authors: Department of Gastroenterology A, La Rabta Hospital - 1007, Tunisia. w.mekki@laposte.net
authors: Lilia
authors: L
authors: Zouiten-Mekki
authors: Maher
authors: M
authors: Kharrat
authors: Sami
authors: S
authors: Karoui
authors: Mariem
authors: M
authors: Serghimi
authors: Monia
authors: M
authors: Fekih
authors: Samira
authors: S
authors: Matri
authors: Lamia
authors: L
authors: Kallel
authors: Jalel
authors: J
authors: Boubaker
authors: Azza
authors: A
authors: Filali
authors: Habiba
authors: H
authors: Chaabouni
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003424:Crohn Disease / Q000208:ethnology* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D014416:Tunisia
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4