[Preliminary study on DNA damage of ZrO(2)/LaPO(4) diphase ceramics on human peripheral blood lymphocytes in vitro].
OBJECTIVE
To detect the genotoxicity of dental machinable ZrO(2)/LaPO(4) diphase ceramics on human peripheral blood lymphocytes in vitro.


METHODS
The evaluation of DNA damage on human lymphocytes was performed by comet assay for three groups of ZrO(2)/LaPO(4) diphase ceramics with 30wt% of LaPO(4) (with 3wt% and 5wt% of Y(2)O(3)) and 40wt% of LaPO(4) (with 5wt% of Y(2)O(3)). The results were analyzed with SPSS16.0 software package for one-factor ANOVA and LSD.


RESULTS
Three experimental groups with different concentration of LaPO(4) of ZrO(2)/LaPO(4) diphase ceramics, the negative control of IPS Empress II ceramics and the blank behaved little migration of the DNA strands respectively after six-day test, and there was no significant difference in all the groups except the positive control (P>0.05).


CONCLUSIONS
The study indicates little effect of DNA damage of ZrO(2)/LaPO(4) diphase ceramics.
Zhu
Hui-fang
HF
Department of Prosthodontics, College of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. hyogawinter@163.com
Chen
Li-ping
LP
Zhang
Xiu-li
XL
Zhang
Bao-wei
BW
D000538:Aluminum Silicates; D002516:Ceramics; D004249:DNA Damage; D003764:Dental Materials; D003776:Dental Porcelain; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes
D000538:Aluminum Silicates; D003764:Dental Materials; C075815:IPS-Empress ceramic; D003776:Dental Porcelain