Pontibacillus litoralis sp. nov., a facultatively anaerobic bacterium isolated from a sea anemone, and emended description of the genus Pontibacillus.
A facultatively anaerobic, moderately halophilic, Gram-positive, endospore-forming, motile, catalase- and oxidase-positive, rod-shaped bacterium, strain JSM 072002(T), was isolated from a sea anemone (Anthopleura xanthogrammica) collected from the South China Sea. Strain JSM 072002(T) was able to grow with 0.5-15 % (w/v) NaCl and at pH 6.0-10.0 and 15-50 degrees C; optimum growth was observed with 2-5 % (w/v) NaCl and at pH 7.5 and 35 degrees C. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C(15 : 0) and anteiso-C(15 : 0). The predominant respiratory quinone was menaquinone 7 and the genomic DNA G+C content was 41.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 072002(T) should be assigned to the genus Pontibacillus and revealed relatively low 16S rRNA gene sequence similarities (<97 %) with the type strains of the three recognized Pontibacillus species (Pontibacillus chungwhensis BH030062(T), 96.8 %; Pontibacillus marinus KCTC 3917(T), 96.7 %; Pontibacillus halophilus JSM 076056(T), 96.0 %). The combination of phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 072002(T) represents a novel species of the genus Pontibacillus, for which the name Pontibacillus litoralis sp. nov. is proposed. The type strain is JSM 072002(T) (=DSM 21186(T)=KCTC 13237(T)). An emended description of the genus Pontibacillus is also presented.
Chen
Yi-Guang
YG
College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, PR China.
Zhang
Yu-Qin
YQ
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Yi
Lang-Bo
LB
College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, PR China.
Li
Zhao-Yang
ZY
College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, PR China.
Wang
Yong-Xiao
YX
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Xiao
Huai-Dong
HD
College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, PR China.
Chen
Qi-Hui
QH
College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, PR China.
Cui
Xiao-Long
XL
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Li
Wen-Jun
WJ
Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
D000693:Anaerobiosis; D031883:Anemone / Q000382:microbiology*; D000818:Animals; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S