PGF isoforms, PLGF-1 and PGF-2, in colorectal cancer and the prognostic significance.
OBJECTIVE
This study examined the expression of PLGF-1 and PGF-2 in human colorectal cancer and how changes in expression may be linked to prognosis of the disease.


METHODS
Expression of PLGF transcripts in primary colorectal tumours (n=94) and background tissue (n=80) were analysed by Q-PCR. Immunohistochemistry was carried out on a number of matched background and tumour sections.


RESULTS
There was a progressive increase in Dukes A to C (430+/-134 versus 674+/-2680) and with TNM 1 to 3 as compared to background tissues (479+/-152 versus 777+/-327 p=0.032). Interestingly, there was an increase in PLGF-1 expression in patients with poor outcome compared with patients remaining disease-free. PGF-2 expression was significantly elevated in tumours compared to normal (4558+/-240 versus 859+/-67.3 p<0.0001). Moreover, expression was increased in patients with poor prognosis compared to those remaining disease free (4585+/-285 versus 3909+/-446).


CONCLUSIONS
There is increased expression of the PLGF isoforms in human colorectal cancer.
Escudero-Esparza
Astrid
A
Metastasis & Angiogenesis Research Group, Department of Surgery, Cardiff School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK. escudero-esparzaa@cardiff.ac.uk
Martin
Tracey A
TA
Davies
Mansel Leigh
ML
Jiang
Wen G
WG
D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016896:Treatment Outcome
C000608036:PGF protein, human; D011257:Pregnancy Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D000072483:Placenta Growth Factor