Genotyping of Candida albicans by fragment analysis of microsatellites combined with 25S rDNA and RPS-based strategies.
Because of its high discriminatory potential, fragment analysis of microsatellites has been frequently used for genotyping of Candida albicans at the strain level. In order to evaluate a genotyping system based on the fragment analysis of microsatellites combined with PCRs targeting 25S rDNA and RPS, 456 independent strains of C. albicans were subjected to genotype analysis using 4 microsatellite markers (CDC3, HIS3, CA I and CA III), followed by 25S rDNA and RPS-based genotyping. The fragment analysis using CA I showed the highest discriminatory potential (DP=0.9782), followed by HIS3 (DP=0.8780). Using combined microsatellite markers, 456 C. albicans strains were divided into 384 genotypes (DP=0.9984). PCRs targeting 25S rDNA and RPS were performed to differentiate the strains that showed identical genotypes in the fragment analysis, resulting in 434 genotypes (DP=0.9996). The combined genotyping system showed high discriminatory power at the strain level, and therefore is useful for rapid genotyping in molecular epidemiological studies of candidiasis.
Adachi
Hidesada
H
Department of Dermatology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Shimizu
Kazue
K
Hattori
Hisao
H
Tanaka
Reiko
R
Chibana
Hiroji
H
Takagi
Yuki
Y
Tomita
Yasushi
Y
Kanbe
Toshio
T
D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S