A third functional isoform enriched in mushroom body neurons is encoded by the Drosophila 14-3-3zeta gene.
14-3-3 proteins are highly conserved across eukaryotes, typically encoded by multiple genes in most species. Drosophila has only two such genes, 14-3-3zeta (leo), encoding two isoforms LEOI and LEOII, and 14-3-3epsilon. We report a bona fide third functional isoform encoded by leo divergent from the other two in structurally and functionally significant areas, thus increasing 14-3-3 diversity in Drosophila. Furthermore, we used a novel approach of spatially restricted leo abrogation by RNA-interference and revealed differential LEO distribution in adult heads, with LEOIII enrichment in neurons essential for learning and memory in Drosophila.
Messaritou
Georgia
G
Institute of Molecular Biology and Genetics, Biomedical Sciences Research Centre Alexander Fleming, Vari, Greece.
Leptourgidou
Flora
F
Franco
Maribel
M
Skoulakis
Efthimios M C
EM
D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D048948:14-3-3 Proteins; D029721:Drosophila Proteins; D020033:Protein Isoforms