[Effect of 5F from Pteris semipinnata on expression of Nr1d1 in HO-8910PM cell line].
OBJECTIVE
To investigate the effects of PsL5F (ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid, an extract from Pteris semipinnata L) on the expression of nuclear receptor subfamily 1, group D, member 1 (Nr1d1) in highly metastatic ovarian carcinoma HO-8910PM cells, and its mechanisms.


METHODS
Microarray Chip was used to examine the level of Nr1d1 mRNA expression on HO-8910PM cells treated with PsL5F. Fluorescent quantitative real-time PCR assay and Western blot were performed to verify the effects of PsL5F on Nr1d1 mRNA and protein expression.


RESULTS
After 24 h treatment of 100 micromol x L(-1) PsL5F, the mRNA and protein levels of Nr1d1 in HO-8910PM cells were 35.34 +/- 1.07 and 7.71 +/- 0.43 times compared to those of control group (P < 0.01, P < 0.01), respectively.


CONCLUSIONS
PsL5F can up-regulate significantly the expression of Nr1d1 in HO-8910PM cells. Antitumor effects and its mechanisms of PsL5F in HO-8910PM cells may be involved in the up-regulation of Nr1d1 expression.
He
Taiping
T
School of Laboratory, Guangdong Medical College, Zhanjiang 524023, China.
Wu
Kefeng
K
Lv
Yingnian
Y
Gong
Xianling
X
Chen
George Gong
GG
Liang
Nianci
N
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000473:pathology; D010881:Piperidones / Q000494:pharmacology*; D032495:Pteris / Q000737:chemistry*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism
C446054:3-(undec-10-enoylamino)glutarimide; D000972:Antineoplastic Agents, Phytogenic; D010881:Piperidones; D012333:RNA, Messenger