title: Toxicity of hydrolyzed vicilins toward Callosobruchus maculatus and phytopathogenic fungi.
abstract: Studies have shown that vicilins (7S storage proteins) from seeds were able to bind to the surface of the Callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of Diatraea saccharalis and Tenebrio molitor , inhibiting larval development. Vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. The present work studies the association of peptides from vicilins of genotypes of Vigna unguiculata (susceptible and resistant to bruchid) with acetylated chitin and the toxicity of vicilin fragments and chitin-binding vicilin fragments to C. maculatus and phytopathogenic fungi. Hydrolysis of vicilins with alpha-chymotrypsin results in a complex mixture of fragments that were separated by chitin-affinity chromatography. Chitin-binding peptides from both genotypes were toxic to C. maculatus larvae, and alpha-chymotrypsin-hydrolyzed vicilins were deleterious to the above insect and to Fusarium oxysporum , Colletotrichum musae , and Saccharomyces cerevisiae fungi.
authors: Universidade Estadual do Norte Fluminense, UENF Parque California, Campos dos Goytacazes, RJ, Brazil.
authors: Adriana Ferreira
authors: AF
authors: Uchôa
authors: Maria Raquel Alcântara
authors: MR
authors: de Miranda
authors: Amanda Jardim
authors: AJ
authors: de Souza
authors: Valdirene Moreira
authors: VM
authors: Gomes
authors: Kátia Valevski Sales
authors: KV
authors: Fernandes
authors: Francisco José Alves
authors: FJ
authors: Lemos
authors: Antonia Elenir Amancio
authors: AE
authors: Oliveira
authors: José
authors: J
authors: Xavier-Filho
mesh_terms: D000818:Animals; D002686:Chitin / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D001517:Coleoptera; D020231:Colletotrichum / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects; D007306:Insecticides; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D055314:Seed Storage Proteins / Q000378:metabolism / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D005659:Fungicides, Industrial; D007306:Insecticides; D010446:Peptide Fragments; D055314:Seed Storage Proteins; D002686:Chitin; C015575:vicilin protein, plant; D002918:Chymotrypsin; C117608:alpha-chymotrypsin