Filter-exchange PGSE NMR determination of cell membrane permeability.
A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.
Aslund
Ingrid
I
Physical Chemistry, Lund University, Lund, Sweden. ingrid.aslund@fkem1.lu.se
Nowacka
Agnieszka
A
Nilsson
Markus
M
Topgaard
Daniel
D
D000465:Algorithms; D002462:Cell Membrane / Q000737:chemistry*; D002463:Cell Membrane Permeability; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D014867:Water