Virgibacillus byunsanensis sp. nov., isolated from a marine solar saltern.
A Gram-variable, motile, endospore-forming and rod-shaped bacterial strain, ISL-24(T), was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by a polyphasic study. Strain ISL-24(T) grew optimally at pH 7.0-8.0, at 30-37 degrees C and in the presence of 8 % (w/v) NaCl. It contained MK-7 as the predominant menaquinone and anteiso-C(15 : 0) as the predominant fatty acid. The DNA G+C content was 37.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-24(T) fell within the genus Virgibacillus, clustering with Virgibacillus carmonensis LMG 20964(T) and Virgibacillus necropolis LMG 19488(T), with a bootstrap resampling value of 92.3 %, and exhibiting 97.3 and 97.4 % 16S rRNA gene sequence similarity, respectively, to these strains. Strain ISL-24(T) exhibited 94.8-96.8 % 16S rRNA gene sequence similarity to the type strains of the other Virgibacillus species. Mean DNA-DNA relatedness values between strain ISL-24(T) and V. carmonensis DSM 14868(T) and V. necropolis DSM 14866(T) were 11 and 19 %, respectively. Differential phenotypic properties of strain ISL-24(T), together with the phylogenetic and genetic distinctiveness, revealed that this strain is different from recognized Virgibacillus species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-24(T) represents a novel species of the genus Virgibacillus, for which the name Virgibacillus byunsanensis sp. nov. is proposed. The type strain is ISL-24(T) (=KCTC 13259(T) =CCUG 56754(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Kang
So-Jung
SJ
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Jung
Yong-Taek
YT
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Lee
Keun Chul
KC
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Oh
Hyun Woo
HW
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000502:physiology; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007723:Korea; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000737:chemistry; D012623:Seawater / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts