title: Morphological, chemical and genetic differentiation of two subspecies of Cistus creticus L. (C. creticus subsp. eriocephalus and C. creticus subsp. corsicus).
abstract: Cistus creticus L., an aromatic species from the Mediterranean area, contains various diterpenes bearing the labdane skeleton. The production of essential oil from this species has potential economic value, but so far, it has not been optimized. In order to contribute to a better knowledge of this species and to its differentiation, the morphological characters, volatile chemical composition and genetic data of two subspecies (C. creticus subsp. eriocephalus and C. creticus subsp. corsicus) were investigated. The leaf trichomes were studied using scanning electron microscopy. The chemical composition of Corsican essential oil (C. creticus subsp. corsicus) has been reported using GC, GC/MS and 13C NMR; the main constituents were oxygenated labdane diterpenes (33.9%) such as 13-epi-manoyl oxide (18.5%). Using plant material (54 samples) collected from 18 geographically distinct areas of the islands of Corsica and Sardinia, the basis of variation in the headspace solid-phase microextraction volatile fraction and an inter-simple sequence repeat genetic analysis were also examined. It was shown that the two subspecies of C. creticus differed in morphology, essential oil production, volatile fraction composition and genetic data.
authors: UMR-CNRS 6134 SPE, Université de Corse, Laboratoire Chimie des Produits Naturels, 20250 Corti, France. Electronic address: paolini@univ-corse.fr.
authors: Julien
authors: J
authors: Paolini
authors: INSERM, U707, 75012 Paris, France.
authors: Alessandra
authors: A
authors: Falchi
authors: UMR-CNRS 6134 SPE, Université de Corse, Service d'Etude et de Recherche en Microscopie Electronique, 20250 Corti, France.
authors: Yann
authors: Y
authors: Quilichini
authors: UMR-CNRS 6134 SPE, Université de Corse, Laboratoire Chimie des Produits Naturels, 20250 Corti, France.
authors: Jean-Marie
authors: JM
authors: Desjobert
authors: UMR-CNRS 6134 SPE, Université de Corse, Laboratoire de Génétique Moléculaire, 20250 Corti, France.
authors: Marie-Cecile De
authors: MC
authors: Cian
authors: UMR-CNRS 6134 SPE, Université de Corse, Laboratoire de Génétique Moléculaire, 20250 Corti, France.
authors: Laurent
authors: L
authors: Varesi
authors: UMR-CNRS 6134 SPE, Université de Corse, Laboratoire Chimie des Produits Naturels, 20250 Corti, France.
authors: Jean
authors: J
authors: Costa
mesh_terms: D001483:Base Sequence; D029758:Cistus / Q000235:genetics*; D004224:Diterpenes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic
chemical_list: D004224:Diterpenes