Rapid detection of conotoxin SO(3) in serum using Cu-chelated magnetic beads coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
A novel method based on Cu-chelated magnetic beads (Cu-Magbeads) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was developed for the rapid detection of peptide toxins in serum. The peptides in the serum were efficiently adsorbed by the Cu-Magbeads, eluted with methanol solution, and assayed by MALDI-TOF-MS. Specific peptides were identified according to their characteristic mass-to-charge ratio values. Conotoxin SO(3), a synthesized peptide, was used as a model to evaluate the method. Conotoxin SO(3) was detected in human serum, as well as bovine and murine serum, with a detection sensitivity in the low femtomole range. The assay was performed within 40 min, without the need for a specific antibody or an expensive reagent. It shows potential for future use in clinical and emergency rescue practice because of its simplicity, high speed, and high sensitivity.
Fang
Junjian
J
National Center of Biomedical Analysis, Beijing 100039, China.
Dong
Fangting
F
Wang
Na
N
He
Kun
K
Liu
Bingyu
B
Wu
Shengming
S
Li
Ailing
A
Zhang
Xuemin
X
D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry; D020916:Conotoxins / Q000097:blood* / Q000138:chemical synthesis; D003300:Copper / Q000737:chemistry*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008024:Ligands; D008297:Male; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D002614:Chelating Agents; D020916:Conotoxins; D008024:Ligands; D003300:Copper