title: Ecological study of lactic acid microbiota isolated from Tempranillo wines of Castilla-La Mancha.
abstract: The genetic diversity of 339 strains of lactic acid bacteria responsible for the spontaneous malolactic fermentation (MLF) in Tempranillo wines produced in six cellars located in different provinces of Castilla-La Mancha region of Spain was analyzed by using randomly amplified polymorphic DNA (RAPD). The results indicate that Oenococcus oeni was the predominant species in MLF of Tempranillo wines with a scant diversity of other lactic acid bacteria (LAB) species. The presence of a well-adapted cellar microbiota is confirmed, with MLF dominated by a limited number of O. oeni genotypes. Clustering of isolates from samples taken from different provinces showed the existence of coincident genotypes in different cellars of the region. This result suggests that a population of cosmopolitan O. oeni strains exists in the Castilla-La Mancha wine-growing region.
authors: Instituto de la Vid y del Vino de Castilla-La Mancha, Crta. Toledo-Albacete s/n, 13700, Tomelloso (Ciudad Real), Spain.
authors: Pedro Miguel Izquierdo
authors: PM
authors: Cañas
authors: Patricia Ruiz
authors: PR
authors: Pérez
authors: Susana Seseña
authors: SS
authors: Prieto
authors: Maria Llanos Palop
authors: ML
authors: Herreros
mesh_terms: D015373:Bacterial Typing Techniques; D005285:Fermentation; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D007218:Industrial Microbiology; D019344:Lactic Acid / Q000378:metabolism*; D007934:Leuconostoc / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D014920:Wine
chemical_list: D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid