title: Cross-breed comparisons identified a critical 591-kb region for bovine carcass weight QTL (CW-2) on chromosome 6 and the Ile-442-Met substitution in NCAPG as a positional candidate.
abstract: BACKGROUND
  Growth-related traits have been mapped on bovine chromosome 6 (BTA 6) in various bovine breed populations. We previously mapped a significant quantitative trait locus (QTL) for carcass and body weight (CW-2) between 38 and 55 cM on BTA 6 using a Japanese Black half-sib family. Additional QTL mapping studies detected four QTL for body or carcass weight that overlapped with CW-2 in Japanese Black and Japanese Brown half-sib families. To map the region in greater detail, we applied cross-breed comparisons of haplotypes that have been shown to be powerful in canine.
  
  
  RESULTS
  We used 38 microsatellite markers to search for a shared Q (increasing carcass and/or body weight) haplotype within the 17-cM CW-2 region among five sires. Linkage disequilibrium mapping using maternal alleles of the offspring showed that an 815-kb shared Q haplotype was associated with body or carcass weight in both breeds. The addition of 43 single nucleotide polymorphism (SNP) markers narrowed the region to 591 kb containing 4 genes. The SNP changing Ile-442 to Met in NCAPG (chromosome condensation protein G) was significantly associated with carcass weight (p < 1.2 x 10-11) in a large Japanese Black population as well as in the five families. The Q allele of the SNP was also associated with a larger longissimus muscle area and thinner subcutaneous fat thickness in steers of all five families, indicating that the CW-2 locus is pleiotropic and favorable for marker-assisted selection of beef cattle.
  
  
  CONCLUSIONS
  A 591-kb critical region for CW-2 was identified. The SNP changing Ile-442 to Met in NCAPG (chromosome condensation protein G) can be used as a positional candidate of CW-2 for marker-assisted selection.
authors: Shirakawa Institute of Animal Genetics, Japan Livestock Technology Association, Odakura, Nishigo, Fukushima 961-8061, Japan. setogu-k@po.minc.ne.jp
authors: Kouji
authors: K
authors: Setoguchi
authors: Masako
authors: M
authors: Furuta
authors: Takashi
authors: T
authors: Hirano
authors: Tomoko
authors: T
authors: Nagao
authors: Toshio
authors: T
authors: Watanabe
authors: Yoshikazu
authors: Y
authors: Sugimoto
authors: Akiko
authors: A
authors: Takasuga
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics*; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics; D002874:Chromosome Mapping / Q000662:veterinary*; D055106:Genome-Wide Association Study; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D018797:Cell Cycle Proteins