title: Aeromonas fluvialis sp. nov., isolated from a Spanish river.
abstract: A Gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(T), was isolated from a water sample collected from the Muga river, Girona, north-east Spain. Preliminary analysis of the 16S rRNA gene sequence showed that this strain belonged to the genus Aeromonas, the nearest species being Aeromonas veronii (99.5 % similarity, with seven different nucleotides). A polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) showed isolate 717(T) to be an independent phylogenetic line, with Aeromonas sobria, Aeromonas veronii and Aeromonas allosaccharophila as the closest neighbour species. DNA-DNA reassociation experiments and phenotypic analysis identified that strain 717(T) represents a novel species, for which the name Aeromonas fluvialis sp. nov. is proposed, with type strain 717(T) (=CECT 7401(T) =LMG 24681(T)).
authors: Unit of Microbiology, Rovira i Virgili University, IISPV, Sant Llorenç 21, 43201, Reus, Spain.
authors: Anabel
authors: A
authors: Alperi
authors: Molecular Diagnostics Center (MDC), Biomolecular Technologies S.L., and Miguel Hernández University, Orihuela E-03300, Alicante. Spain.
authors: Antonio J
authors: AJ
authors: Martínez-Murcia
authors: Molecular Diagnostics Center (MDC), Biomolecular Technologies S.L., and Miguel Hernández University, Orihuela E-03300, Alicante. Spain.
authors: Arturo
authors: A
authors: Monera
authors: Department of Veterinary Sciences, CECAV-University of Trás-os-Montes e Alto Douro, Vila Real, Portugal.|Molecular Diagnostics Center (MDC), Biomolecular Technologies S.L., and Miguel Hernández University, Orihuela E-03300, Alicante. Spain.
authors: Maria J
authors: MJ
authors: Saavedra
authors: Unit of Microbiology, Rovira i Virgili University, IISPV, Sant Llorenç 21, 43201, Reus, Spain.
authors: Maria J
authors: MJ
authors: Figueras
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D013030:Spain
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S