title: Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity.
abstract: Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.
authors: The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. yukiy@ims.u-tokyo.ac.jp
authors: Yoshikazu
authors: Y
authors: Yuki
authors: Daisuke
authors: D
authors: Tokuhara
authors: Tomonori
authors: T
authors: Nochi
authors: Hiroshi
authors: H
authors: Yasuda
authors: Mio
authors: M
authors: Mejima
authors: Shiho
authors: S
authors: Kurokawa
authors: Yuko
authors: Y
authors: Takahashi
authors: Nobuhiro
authors: N
authors: Kataoka
authors: Ushio
authors: U
authors: Nakanishi
authors: Yukari
authors: Y
authors: Hagiwara
authors: Kohtaro
authors: K
authors: Fujihashi
authors: Fumio
authors: F
authors: Takaiwa
authors: Hiroshi
authors: H
authors: Kiyono
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012275:Oryza / Q000235:genetics / Q000276:immunology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D002772:Cholera Toxin