Characteristics of a novel monoclonal antibody against interleukin-14alpha.
Interleukin-14alpha is cytokine encoded by the plus strand of il-14 gene using exons 3-10. It has been demonstrated in an IL-14-transgenic mice model that IL-14alpha could enhance an immune response to both TD and TI antigens and induce a phenotype that is very similar to SLE and Sjögren's syndrome, indicating that IL-14alpha may play an important role in autoimmune disease. To further study the role of IL-14alpha in human immune diseases, there is an urgent need to generate specific anti-human IL-14alpha monoclonal antibodies. In this study, we immunized BALB/c mice with synthesized human IL-14alpha-C polypeptide and obtained a novel monoclonal antibody, the anti-IL-14alpha-C antibody. Analysis by enzyme-linked immunosorbent assay (ELISA), spot-ELISA, Western blot, and immuoprecipitation revealed that the MAb were specifically against recombinant human IL-14alpha protein, and its subtype and affinity constant (K(aff)) were IgG2a/kappa and 1.007 x 10(8) M(-1), respectively. This antibody might facilitate studies for pathophysiology of autoimmune disease.
Peng
Xiaodong
X
Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
Zhou
Changhua
C
Wei
Dapeng
D
Luo
Zhijuan
Z
Zhang
Chongjie
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001327:Autoimmune Diseases / Q000276:immunology; D005091:Exons; D005260:Female; D006801:Humans; D007107:Immune System; D007074:Immunoglobulin G / Q000737:chemistry; D047468:Immunoprecipitation; D007378:Interleukins / Q000737:chemistry*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009101:Multiple Myeloma / Q000473:pathology; D033921:Vesicular Transport Proteins / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007378:Interleukins; C488100:TXLNA protein, human; C545985:TXLNA protein, mouse; D033921:Vesicular Transport Proteins