Psychrobacter piscatorii sp. nov., a psychrotolerant bacterium exhibiting high catalase activity isolated from an oxidative environment.
A Gram-negative, non-motile, psychrotolerant bacterium exhibiting high catalase activity, designated strain T-3-2(T), was isolated from a drain of a fish-processing plant. Its catalase activity was 12 000 U (mg protein)(-1), much higher than the activity of the other Psychrobacter strains tested. The strain grew at 0-30 degrees C and in the presence of 0-12 % NaCl. The predominant isoprenoid quinone was ubiquinone-8 (Q-8), and C(16 : 1)omega9c and C(18 : 1)omega9c were the predominant cellular fatty acids. The DNA G+C content of strain T-3-2(T) was 43.9 mol%. 16S rRNA gene sequence phylogeny suggested that strain T-3-2(T) is a member of the genus Psychrobacter, with the closest relatives being the type strains of Psychrobacter nivimaris (99.2 % similarity), P. aquimaris (98.7 %) and P. proteolyticus (98.5 %). DNA-DNA hybridization showed less than 65 % relatedness with these strains. A phylogenetic tree based on gyrB gene sequences was more reliable, with higher bootstrap values than the 16S rRNA gene sequence-based tree. The result also differentiated the isolate from previously reported Psychrobacter species. Owing to the significant differences in phenotypic and chemotaxonomic characteristics and the phylogenetic and DNA-DNA relatedness data, the isolate merits classification within a novel species, for which the name Psychrobacter piscatorii sp. nov. is proposed. The type strain is T-3-2(T) (=JCM 15603(T) =NCIMB 14510(T)).
Yumoto
Isao
I
Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.|Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Hirota
Kikue
K
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Kimoto
Hideyuki
H
Department of Bioscience and Technology, School of Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.|Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Nodasaka
Yoshinobu
Y
Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan.
Matsuyama
Hidetoshi
H
Department of Bioscience and Technology, School of Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
Yoshimune
Kazuaki
K
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001482:Base Composition; D002374:Catalase / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005399:Fishes; D005511:Food Handling; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D044096:Psychrobacter / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D002374:Catalase