RNA editing of mitochondrial functional genes atp6 and cox2 in maize (Zea mays L.).
RNA editing of two mitochondrial or organs genes, atp6 and cox2, in different tissues were analyzed using homonucleic but alloplasmic, and homoplasmic but heteronucleic maize (zea mays L.) as experimental materials. A total of 18 and 26 editing sites for atp6 conservative region transcript were identified by direct and clone sequencing, respectively. By direct sequencing 23 and 22 editing sites for cox2 transcript were identified in 48-2 and Huangzaosi nuclear backgrounds, respectively. From the direct sequencing results, the occurrence rates of different transcripts generally increase in sterile lines. It is concluded that RNA editing of atp6 and cox2 might have a certain relationship with maize CMS.
Wang
Jing
J
Maize Research Institute of Sichuan Agricultural University/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education, Ya'an 625014, Sichuan, China.
Cao
Mo Ju
MJ
Pan
Guang Tang
GT
Lu
Yan Li
YL
Rong
Ting Zhao
TZ
D001483:Base Sequence; D051546:Cyclooxygenase 2 / Q000235:genetics*; D050259:Genes, Mitochondrial; D017343:Genes, Plant; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D008969:Molecular Sequence Data; D017393:RNA Editing; D017422:Sequence Analysis, DNA; D003313:Zea mays / Q000235:genetics*
D051546:Cyclooxygenase 2; D025261:Mitochondrial Proton-Translocating ATPases