Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis.
A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.
Eom
Gwang Hyeon
GH
Medical Research Center for Gene Regulation and Department of Pharmacology, Chonnam National University Medical School, Gwangju, Republic of Korea.
Kim
Kabsun
K
Kim
Sung-Mi
SM
Kee
Hae Jin
HJ
Kim
Ji-Young
JY
Jin
Hye Mi
HM
Kim
Ju-Ryoung
JR
Kim
Jung Ha
JH
Choe
Nakwon
N
Kim
Kee-Beom
KB
Lee
Junwon
J
Kook
Hyun
H
Kim
Nacksung
N
Seo
Sang-Beom
SB
D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000254:growth & development*; D014018:Tissue Distribution; D014158:Transcription, Genetic; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D012739:Gonadal Steroid Hormones; D012097:Repressor Proteins; D000076983:Histone Methyltransferases; C543643:PRDM8 protein, mouse; D011495:Histone-Lysine N-Methyltransferase