Sphingosinicella vermicomposti sp. nov., isolated from vermicompost, and emended description of the genus Sphingosinicella.
A Gram-stain-negative, rod-shaped bacterium, designated strain YC7378(T) was isolated from vermicompost (VC) collected at Masan, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain YC7378(T) grew optimally at 30 degrees C and at pH 6.5-8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC7378(T) belongs to the genus Sphingosinicella in the family Sphingomonadaceae. The most closely related strains are Sphingosinicella soli KSL-125(T) (95.7 %), Sphingosinicella xenopeptidilytica 3-2W4(T) (95.6 %) and Sphingosinicella microcystinivorans Y2(T) (95.5 %). Strain YC7378(T) contained ubiquinone Q-10 as the major respiratory quinone system and sym-homospermidine as the major polyamine. The major fatty acids of strain YC7378(T) were C(18 : 1)omega7c, C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, C(14 : 0) 2-OH and C(16 : 0). The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The total DNA G+C content was 59.4 mol%. The phenotypic, phylogenetic and chemotaxonomic data showed that strain YC7378(T) represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella vermicomposti sp. nov. is proposed. The type strain is YC7378(T) (=KCTC 22446(T) =DSM 21593(T)).
Yasir
Muhammad
M
Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Aslam
Zubair
Z
Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Song
Geun Cheol
GC
Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Jeon
Che Ok
CO
Department of Life Sciences, Chung-Ang University, Seoul 156-756, Korea.
Chung
Young Ryun
YR
Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S