Dactylosporangium maewongense sp. nov., isolated from soil.
Morphological and chemotaxonomic characterization of actinomycete strain MW2-25(T), isolated from tropical forest soil in Nakhon Sawan Province, Thailand, clearly demonstrated that this strain belongs to the genus Dactylosporangium. Phylogenetic analysis using 16S rRNA gene sequences also indicated that this strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium aurantiacum IFO 12592(T) (99.3 % sequence similarity). DNA-DNA hybridization values and some physiological and biochemical properties indicated that this strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Dactylosporangium maewongense sp. nov. is proposed. The type strain is MW2-25(T) (=BCC 34832(T)=JCM 15933(T)).
Chiaraphongphon
Suthamat
S
Department of Applied Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Suriyachadkun
Chanwit
C
BIOTEC Culture Collection, BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
Tamura
Tomohiko
T
Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0812, Japan.
Thawai
Chitti
C
Department of Applied Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D013785:Thailand
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S