title: Halomonas stevensii sp. nov., Halomonas hamiltonii sp. nov. and Halomonas johnsoniae sp. nov., isolated from a renal care centre.
abstract: A total of 14 Halomonas strains were isolated from the blood of two patients and from dialysis machines of a renal care centre. The strains were Gram-negative, halophilic, motile and non-spore-forming rods. They produced cream-coloured colonies and contained Q-9 as the predominant ubiquinone and C(18 : 1)omega7c and C(16 : 0) as the major fatty acids. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the 14 isolates were most closely related to Halomonas magadiensis 21 MI(T) with 98.1-98.9 % sequence similarity and that they formed three separate lineages among themselves. Combined phenotypic and DNA-DNA hybridization data support the conclusion that they represent three novel species of the genus Halomonas, for which the names Halomonas stevensii sp. nov. (type strain S18214(T)=KCTC 22148(T)=DSM 21198(T)), Halomonas hamiltonii sp. nov. (type strain W1025(T)=KCTC 22154(T)=DSM 21196(T)) and Halomonas johnsoniae sp. nov. (type strain T68687(T)=KCTC 22157(T)=DSM 21197(T)) are proposed.
authors: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Kwang Kyu
authors: KK
authors: Kim
authors: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Keun Chul
authors: KC
authors: Lee
authors: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Hee-Mock
authors: HM
authors: Oh
authors: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Jung-Sook
authors: JS
authors: Lee
mesh_terms: D016470:Bacteremia / Q000209:etiology / Q000382:microbiology*; D001482:Base Composition; D001483:Base Sequence; D002140:California; D004269:DNA, Bacterial / Q000737:chemistry; D004865:Equipment and Supplies, Hospital / Q000382:microbiology*; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006436:Hemodialysis Units, Hospital; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D006435:Renal Dialysis / Q000295:instrumentation*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S