[Delayed effect of isoflurane on hippocampal proteome after anesthesia in adult and aged rats].
OBJECTIVE
To investigate the delayed alteration of hippocampus proteome after anesthesia with isoflurane in adult and aged rats.


METHODS
Ten 8-month-old SD rats were randomly divided into group C adult and group I adult (5 in each group), and another ten 22-month-old SD rats were randomly divided into group C aged and group I aged (5 in each group).The rats in group I adult and group I aged received 2 h anesthesia with 1.2% isoflurane. The rats in group C adult and group C aged inhaled 40% oxygen for contrast. The hippocampal proteome of each rat was measured by 2-dimensional gel electrophoresis and mass spectrometry.


RESULTS
The vital signs of the rats in group I adult and group I aged were stable. There were 878+/-34 protein spots in group C adult, 864+/-49 protein spots in group I adult, 834+/-47 in group C aged, and 819+/-24 in group I aged. There were 12(4/8)different protein spots between group I adult and group C adult. There were 11(3/8)different protein spots between group I aged and group C aged. All of the protein spots were identified by MALDI-TOF-MS. Most of the different proteins were related to metabolism, anti-oxidation, and signal conditioning of synapse.


CONCLUSIONS
Isoflurane may cause the alteration of hippocampal proteome in rats, which is age-related.
Duan
Kaiming
K
Department of Anesthesiology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Ouyang
Wen
W
Chen
Manhong
M
Xia
Yuefeng
Y
Wang
Saiying
S
D000367:Age Factors; D000375:Aging; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D007530:Isoflurane / Q000494:pharmacology*; D013058:Mass Spectrometry; D020543:Proteome / Q000187:drug effects / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D018685:Anesthetics, Inhalation; D020543:Proteome; D007530:Isoflurane