title: Acid-sensing ionic-channel functional expression in the vestibular endorgans.
abstract: In the vestibular system, the electrical discharge of the afferent neurons has been found to be highly sensitive to external pH changes, and acid-sensing ionic-channels (ASIC) have been found to be functionally expressed in afferent neurons. No previous attempt to assay the ASIC function in vestibular afferent neurons has been done. In our work we studied the electrical discharge of the afferent neuron of the isolated inner ear of the axolotl (Ambystoma tigrinum) to determine the participation of proton-gated currents in the postransductional information processing in the vestibular system. Microperfusion of FMRF-amide significantly increased the resting activity of the afferent neurons of the semicircular canal indicating that ASIC currents are tonically active in the resting condition. The use of ASIC antagonists, amiloride and acetylsalicylic acid (ASA), significantly reduced the vestibular-nerve discharge, corroborating the idea that the afferent neurons of the vestibular system express ASICs that are sensitive to amiloride, ASA, and to FMRF-amide. The sensitivity of the vestibular afferent-resting discharge to the microperfusion of ASIC acting agents indicates the participation of these currents in the establishment of the afferent-resting discharge.
authors: Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, Apartado Postal 406, Puebla, Pue. 72000, Mexico. axolotl@siu.buap.mx
authors: Rosario
authors: R
authors: Vega
authors: Uxmal
authors: U
authors: Rodríguez
authors: Enrique
authors: E
authors: Soto
mesh_terms: D062565:Acid Sensing Ion Channels; D000557:Ambystoma; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D004594:Electrophysiology; D019835:FMRFamide / Q000494:pharmacology; D005682:Gadolinium / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000502:physiology; D009475:Neurons, Afferent / Q000378:metabolism*; D015222:Sodium Channels / Q000096:biosynthesis* / Q000502:physiology; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000378:metabolism*
chemical_list: D062565:Acid Sensing Ion Channels; D009419:Nerve Tissue Proteins; D015222:Sodium Channels; D019835:FMRFamide; D000584:Amiloride; D005682:Gadolinium; C038958:gadolinium chloride; D001241:Aspirin