Influence of an aerobic fungus grown on solid culture on ruminal degradability and on a mixture culture of anaerobic cellulolytic bacteria.
In this work, the effect of a solid fungal culture of Aspergillus niger (An) grown on coffee pulp on the in situ ruminal degradability (RD) of corn stover was evaluated. In addition, the effect of its extracts on the in vitro dry matter disappearance (IVDMD) and on a mixed culture of anaerobic cellulolytic bacteria (MCACB) was also investigated. The solid ferment was a crude culture of An, grown on coffee pulp. Regarding in situ RD, a significant difference (p < 0.05) was found between treatment with 200 g/day of the solid culture and control (no solid culture added) on dry matter, crude protein and neutral detergent fibre on RD. All the water extracts (pH 4, 7 and 10) enhanced IVDMD and stimulated the cellulolytic activity on a MCACB. Ultrafiltration results showed that active compounds with a molecular weight lower than 30 kDa were responsible for the effect on MCACB. Such results suggest that the effects of the solid An culture in RD are related to the presence of water soluble compounds having a molecular weight lower than 30 kDa.
Hernández-Díaz
R
R
ICAP, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km. 1, Rancho Universitario, Tulancingo, Hgo., México.
Pimentel-González
D J
DJ
Figueira
A C
AC
Viniegra-González
G
G
Campos-Montiel
R G
RG
D000332:Aerobiosis; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001234:Aspergillus niger / Q000502:physiology*; D001421:Bacteria, Anaerobic / Q000502:physiology*; D002417:Cattle / Q000502:physiology*; D002482:Cellulose / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004063:Digestion; D005260:Female; D012417:Rumen / Q000502:physiology*; D003313:Zea mays
D002482:Cellulose