Schistosoma mansoni infection of juvenile Biomphalaria glabrata induces a differential stress response between resistant and susceptible snails.
Schistosomes develop successfully in susceptible snails but are encapsulated and killed in resistant ones. Mechanism(s) shaping these outcomes involves the parasites ability to evade the snail's defenses. RNA analysis from resistant (BS-90), non-susceptible (LAC2) and susceptible (NMRI) juvenile Biomphalaria glabrata to Schistosoma mansoni revealed that stress-related genes, heat shock protein 70 (Hsp 70) and reverse transcriptase (RT), were dramatically co-induced early in susceptible snails, but not in resistant/non-susceptible ones. These transcripts were, however, down regulated upon exposure to irradiated parasites although penetration behavior of irradiated vs. normal parasites were the same, indicating that Hsp 70 and RT regulation was elicited by infection and not injury. Understanding molecular events involved in stress response transcriptional regulation of Hsp 70 in juvenile snails could pave a way towards the identification of genes involved in schistosome/snail interactions.
Ittiprasert
Wannaporn
W
Biomedical Research Institute, Rockville, MD 20852, USA.
Nene
Rahul
R
Miller
André
A
Raghavan
Nithya
N
Lewis
Fred
F
Hodgson
Jacob
J
Knight
Matty
M
D000818:Animals; D001700:Biomphalaria / Q000235:genetics / Q000276:immunology* / Q000469:parasitology*; D015536:Down-Regulation / Q000276:immunology; D005720:Gamma Rays; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000276:immunology / Q000502:physiology* / Q000528:radiation effects; D015533:Transcriptional Activation / Q000276:immunology
D018840:HSP70 Heat-Shock Proteins; D012194:RNA-Directed DNA Polymerase