Differential effect of PYY1-36 and PYY3-36 on gastric emptying in man.
Peptide tyrosine-tyrosine (PYY) is a prandially controlled hormone in endocrine ileal and colonic mucosa cells. In plasma, PYY appears as full-length PYY1-36 and truncated PYY3-36. Both have different pharmacological profile, and PYY3-36 seems to inhibit food intake. We aimed at investigating the effect of intravenously administered PYY1-36 and PYY3-36 on gastric emptying and short-term metabolic control. Eight healthy adults were studied in single-blinded, randomized design. At separate occasions, intravenous infusion of saline, PYY1-36 or PYY3-36 (0.8 pmol kg(-1) min(-1)) and a radio-labelled omelette were given. Gastric emptying (scintigraphy), appetite ratings (VAS), and plasma concentrations of insulin, glucose, GLP-1 and PYY were measured. PYY3-36 and PYY1-36 both inhibited gastric emptying, PYY3-36 most effectively. Half-emptying time was prolonged from 63.1+/-5.2 (saline) to 87.0+/-11.5 min (PYY3-36), whereas retention at 120 min was 2.5+/-1.4% for saline, 10.7+/-4.4 for PYY1-36 and 15.8+/-4.4 for PYY3-36. Neither form influenced glucose or GLP-1 concentrations, but both decreased the postprandial rise in insulin. PYY3-36 induced nausea (VAS increase 47.5+/-22.6 mm) and decreased prospective consumption (VAS change 39.5+/-7.7 mm). In conclusion, PYY3-36's reducing effect upon food intake might be mediated by a decreased gastric emptying rate.
Witte
A-B
AB
Department of Medicine, Unit of Gastroenterology and Hepatology, Karolinska University Hospital Solna, Karolinska Institutet, 171 76 Stockholm, Sweden.
Grybäck
P
P
Holst
J J
JJ
Hilsted
L
L
Hellström
P M
PM
Jacobsson
H
H
Schmidt
P T
PT
D000328:Adult; D001066:Appetite / Q000187:drug effects; D001786:Blood Glucose / Q000032:analysis; D005260:Female; D005746:Gastric Emptying / Q000187:drug effects*; D052216:Glucagon-Like Peptide 1 / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D009325:Nausea / Q000139:chemically induced; D010446:Peptide Fragments; D019894:Peptide YY / Q000009:adverse effects / Q000097:blood / Q000494:pharmacology*; D016037:Single-Blind Method
D001786:Blood Glucose; D007328:Insulin; D010446:Peptide Fragments; D019894:Peptide YY; C061785:peptide YY (3-36); D052216:Glucagon-Like Peptide 1; C514549:peptide YY (1-36)