Gastroprotection of (-)-alpha-bisabolol on acute gastric mucosal lesions in mice: the possible involved pharmacological mechanisms.
(-)-Alpha-Bisabolol is an unsaturated, optically active sesquiterpene alcohol obtained by the direct distillation essential oil from plants such as Vanillosmopsis erythropappa and Matricaria chamomilla. (-)-Alpha-Bisabolol has generated considerable economic interest, since it possesses a delicate floral odor and has been shown to have anti-septic and anti-inflammatory activity. The aim of this work was to evaluate the gastroprotective action of (-)-alpha-bisabolol on ethanol and indomethacin-induced ulcer models in mice, and further investigate the pharmacological mechanisms involved in this action. The oral administration of (-)-alpha-bisabolol 100 and 200 mg/kg was able to protect the gastric mucosa from ethanol (0.2 mL/animal p.o.) and indomethacin-induced ulcer (20 mg/kg p.o.). Administration of L-NAME (10 mg/kg i.p.), glibenclamide (10 mg/kg i.p.) or indomethacin (10 mg/kg p.o.) was not able to revert the gastroprotection promoted by (-)-alpha-bisabolol 200 mg/kg on the ethanol-induced ulcer. Dosage of gastric reduced glutathione (GSH) levels showed that ethanol and indomethacin reduced the content of non-protein sulfhydryl (NP-SH) groups, while (-)-alpha-bisabolol significantly decreased the reduction of these levels on ulcer-induced mice, but not in mice without ulcer. In conclusion, gastroprotective effect on ethanol and indomethacin-induced ulcer promoted by (-)-alpha-bisabolol may be associated with an increase of gastric sulfydryl groups bioavailability leading to a reduction of gastric oxidative injury induced by ethanol and indomethacin.
Moura Rocha
Nayrton Flávio
NF
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Rua Cel Nunes de Melo 1127, CEP 60430-270 Fortaleza, Brazil.
Venâncio
Edith Teles
ET
Moura
Brinell Arcanjo
BA
Gomes Silva
Maria Izabel
MI
Aquino Neto
Manoel Rufino
MR
Vasconcelos Rios
Emiliano Ricardo
ER
de Sousa
Damião Pergentino
DP
Mendes Vasconcelos
Silvânia Maria
SM
de França Fonteles
Marta Maria
MM
de Sousa
Francisca Cléa Florenço
FC
D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D007213:Indomethacin / Q000633:toxicity; D008297:Male; D051379:Mice; D023421:Models, Animal; D000081222:Monocyclic Sesquiterpenes; D010437:Peptic Ulcer / Q000473:pathology / Q000517:prevention & control*; D012717:Sesquiterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000897:Anti-Ulcer Agents; D000081222:Monocyclic Sesquiterpenes; D012717:Sesquiterpenes; D013438:Sulfhydryl Compounds; C004497:bisabolol; D000431:Ethanol; D005978:Glutathione; D007213:Indomethacin