title: Structural characterisation of the pectic polysaccharide rhamnogalacturonan II using an acidic fingerprinting methodology.
abstract: Rhamnogalacturonan II (RG-II) is a structurally complex cell wall pectic polysaccharide. Despite its complexity, both the structure of RG-II and its ability to dimerise via a borate diester are conserved in vascular plants suggesting that RG-II has a fundamental role in primary cell wall organisation and function. The selection and analysis of new mutants affected in RG-II formation represents a promising strategy to unravel these functions and to identify genes encoding enzymes involved in RG-II biosynthesis. In this paper, a novel fingerprinting strategy is described for the screening of RG-II mutants based on the mild acid hydrolysis of RG-II coupled to the analysis of the resulting fragments by mass spectrometry. This methodology was developed using RG-II fractions isolated from citrus pectins and then validated for RG-II isolated from the Arabidopsis mur1 mutant and irx10 irx10-like double mutant.
authors: EA 4358, IFRMP 23, University of Rouen, 76821 Mont Saint Aignan, France.
authors: Martial
authors: M
authors: Séveno
authors: Aline
authors: A
authors: Voxeur
authors: Christophe
authors: C
authors: Rihouey
authors: Ai-Min
authors: AM
authors: Wu
authors: Tadashi
authors: T
authors: Ishii
authors: Christian
authors: C
authors: Chevalier
authors: Marie Christine
authors: MC
authors: Ralet
authors: Azeddine
authors: A
authors: Driouich
authors: Alan
authors: A
authors: Marchant
authors: Patrice
authors: P
authors: Lerouge
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D002957:Citrus / Q000737:chemistry*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D013696:Temperature; D014269:Trifluoroacetic Acid / Q000737:chemistry*
chemical_list: C042492:rhamnogalacturonan II; D010368:Pectins; D014269:Trifluoroacetic Acid