title: Adiponectin and adiponectin receptors in human pheochromocytoma.
abstract: OBJECTIVE
  Recent studies have demonstrated that serum adiponectin and its receptors in adipose and muscle tissues are suppressed in diabetic or obese individuals. Patients with pheochromocytoma are frequently diabetic.
  
  
  METHODS
  Using real-time PCR, we examined mRNA expressions of adiponectin (Adp) and adiponectin receptor 1 (AdpR1) and AdpR2 in pheochromocytoma tissues from 49 patients. We also measured levels of serum total and high molecular weight (HMW) adiponectin levels in 10 pheochromocytomas and 33 normal volunteers.
  
  
  RESULTS
  In pheochromocytoma tissue, AdpR1 mRNA expression was higher in adrenaline (A)-type tumors than in noradrenaline (NA)-type tumors. AdpR1 expression was significantly higher in A-type non-diabetics than in NA-type non-diabetics (p<0.05). AdpR1 mRNA expression was significantly associated with the tumor tissue adrenaline content (p<0.005) in linear regression analysis, which suggest that adrenaline positively regulates AdpR1 mRNA expression.Serum total and HMW Adp levels in patients with NA-type pheochromocytomas were approximately 3 times higher than those of healthy volunteers. After adrenalectomy, levels of adiponectin normalized.
  
  
  CONCLUSIONS
  Our results indicate that serum total and HMW Adp, and AdpR1 gene expressions in pheochromocytoma tissue, are associated with the level of catecholamine produced in the tumor. It is tempting to speculate that catecholamine induces adiponectin production and signaling.
authors: Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan. k-isobe@md.tsukuba.ac.jp
authors: Kazumasa
authors: K
authors: Isobe
authors: Ling
authors: L
authors: Fu
authors: Ichirou
authors: I
authors: Tatsuno
authors: Hideto
authors: H
authors: Takahashi
authors: Sumiko
authors: S
authors: Nissato
authors: Hisato
authors: H
authors: Hara
authors: Toru
authors: T
authors: Yashiro
authors: Kazumi
authors: K
authors: Suzukawa
authors: Kazuhiro
authors: K
authors: Takekoshi
authors: Hitoshi
authors: H
authors: Shimano
authors: Yasushi
authors: Y
authors: Kawakami
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000310:Adrenal Gland Neoplasms / Q000737:chemistry*; D000328:Adult; D016022:Case-Control Studies; D002395:Catecholamines / Q000032:analysis / Q000096:biosynthesis; D004837:Epinephrine / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010673:Pheochromocytoma / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D054419:Receptors, Adiponectin / Q000235:genetics*
chemical_list: C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D052242:Adiponectin; D002395:Catecholamines; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D004837:Epinephrine