title: Outer membrane VDAC1 controls permeability transition of the inner mitochondrial membrane in cellulo during stress-induced apoptosis.
abstract: Voltage-dependent anion channel (VDAC)1 is the main channel of the mitochondrial outer membrane (MOM) and it has been proposed to be part of the permeability transition pore (PTP), a putative multiprotein complex candidate agent of the mitochondrial permeability transition (MPT). Working at the single live cell level, we found that overexpression of VDAC1 triggers MPT at the mitochondrial inner membrane (MIM). Conversely, silencing VDAC1 expression results in the inhibition of MPT caused by selenite-induced oxidative stress. This MOM-MIM crosstalk was modulated by Cyclosporin A and mitochondrial Cyclophilin D, but not by Bcl-2 and Bcl-X(L), indicative of PTP operation. VDAC1-dependent MPT engages a positive feedback loop involving reactive oxygen species and p38-MAPK, and secondarily triggers a canonical apoptotic response including Bax activation, cytochrome c release and caspase 3 activation. Our data thus support a model of the PTP complex involving VDAC1 at the MOM, and indicate that VDAC1-dependent MPT is an upstream mechanism playing a causal role in oxidative stress-induced apoptosis.
authors: INSERM U916, Université Bordeaux 2, Institut Bergonié, 33076 Bordeaux, France.
authors: Flora
authors: F
authors: Tomasello
authors: Angela
authors: A
authors: Messina
authors: Lydia
authors: L
authors: Lartigue
authors: Laura
authors: L
authors: Schembri
authors: Chantal
authors: C
authors: Medina
authors: Simona
authors: S
authors: Reina
authors: Didier
authors: D
authors: Thoraval
authors: Marc
authors: M
authors: Crouzet
authors: François
authors: F
authors: Ichas
authors: Vito
authors: V
authors: De Pinto
authors: Francesca
authors: F
authors: De Giorgi
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D019556:COS Cells; D002463:Cell Membrane Permeability / Q000502:physiology; D002522:Chlorocebus aethiops; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D025461:Feedback, Physiological / Q000502:physiology; D020868:Gene Silencing / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D018038:Sodium Selenite / Q000494:pharmacology; D013312:Stress, Physiological / Q000502:physiology*; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D000081406:Peptidyl-Prolyl Isomerase F; C494762:VDAC1 protein, human; D016572:Cyclosporine; D050995:Voltage-Dependent Anion Channel 1; D021983:Cyclophilins; D018038:Sodium Selenite