Kazachstania taianensis sp. nov., a novel ascomycetous yeast species from orchard soil.
Three teleomorphic ascomycetous yeast isolates (TA11TR-1(T), TA11TR-4 and TA11TR-6) from orchard soil from Tai'an, Shandong province, China, were shown to represent a novel species within the genus Kazachstania based on phenotypic characterization and sequence analyses of the 18S rRNA gene, internal transcribed spacer (ITS) regions and 26S rDNA gene D1/D2 domain. The name Kazachstania taianensis sp. nov. (type strain TA11TR-1(T) =AS 2.4160(T) =CBS 11405(T)) is proposed. K. taianensis sp. nov. clustered in a branch together with Kazachstania sinensis, Kazachstania naganishii and the Kazachstania telluris complex with moderate bootstrap support in the neighbour-joining tree reconstructed from combined 18S and D1/D2 sequences. The novel species possessed unusual ITS 1 (338 bp) and ITS 2 (488 bp) sequences. The total length of the ITS-5.8S rDNA gene region of the species was 983 bp, being much longer than those of other ascomycetous yeast species described so far.
Chen
Ru
R
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
Wei
Shao-Chong
SC
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
Jiang
Yuan-Mao
YM
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, PR China.
Wang
Qi-Ming
QM
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
Bai
Feng-Yan
FY
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D005638:Fruit; D007621:Karyotyping; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity; D014197:Trees
D012331:RNA, Fungal; D012336:RNA, Ribosomal, 16S