Agromyces atrinae sp. nov., isolated from fermented seafood.
A Gram-staining-positive, aerobic, non-motile and rod-shaped bacterium, designated P27(T), was isolated from a traditional fermented seafood. The isolate grew optimally with 0-2.0 % (w/v) NaCl and at pH 6-7 and 30 degrees C. The predominant menaquinones were MK-12 and MK-11. The major cellular fatty acids were anteiso-C(17 : 0), anteiso-C(15 : 0) and iso-C(16 : 0). The major cell-wall sugars were galactose, mannose and rhamnose. The peptidoglycan amino acids of strain P27(T) were 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The genomic DNA G+C content of strain P27(T) was 69.0 mol%. Based on its 16S rRNA gene sequence, strain P27(T) showed highest pairwise similarity with Agromyces cerinus subsp. cerinus JCM 9083(T) (97.0 % similarity). Based on phenotypic, genotypic and phylogenetic studies, strain P27(T) represents a novel species in the genus Agromyces, for which the name Agromyces atrinae sp. nov. is proposed. The type strain is P27(T) (=KCTC 19593(T) =JCM 15913(T)).
Park
Eun-Jin
EJ
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Kim
Min-Soo
MS
UST, Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.|Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Jung
Mi-Ja
MJ
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Roh
Seong Woon
SW
UST, Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.|Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Chang
Ho-Won
HW
UST, Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
Shin
Kee-Sun
KS
UST, Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
Bae
Jin-Woo
JW
UST, Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.|Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017747:Seafood / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S