title: Full-length normalization subtractive hybridization: a novel method for generating differentially expressed cDNAs.
abstract: Here we present a novel method termed full-length normalization subtractive hybridization (FNSH) for efficiently generating subtracted cDNA libraries with a high degree of productivity. This method has the ability to isolate full-length differentially expressed genes from target samples. Normalization and subtraction of FNSH are performed simultaneously with efficiency equal to or even higher than that of suppression subtractive hybridization. Using FNSH, we have isolated at least 40 unique cDNAs that are expressed in terminal ampullae but not in the ovaries of the prawn Macrobrachium rosenbergii from 120 randomly picked subtracted clones. Sequence analysis shows that 37 of the 40 cDNAs are full length.
authors: Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang, 310058, People's Republic of China.
authors: Zhong-Min
authors: ZM
authors: Dai
authors: Xiao-Jing
authors: XJ
authors: Zhu
authors: Wei-Jun
authors: WJ
authors: Yang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D033541:Palaemonidae / Q000235:genetics; D013382:Subtraction Technique; D014649:Vas Deferens / Q000378:metabolism
chemical_list: D018076:DNA, Complementary