title: Peptide microarrays on bisphenol A polycarbonate.
abstract: Microarrays are frequently prepared on microscope glass slides. However, glass substrates can break or cut and thus can lead to the contamination of the manipulator during the analysis of biological samples. Alternately, bisphenol A polycarbonate (PC) is shock-resistant and, in addition, is easily eliminated by incineration. We show here that PC is a useful substrate for peptide microarray preparation. We describe in particular the preparation of peptide microarrays on PC using semicarbazide-functionalized silica nanoparticles and in situ semicarbazone ligation with glyoxylyl-peptides. The microarrays were used for the detection of antibodies using fluorescence detection.
authors: UMR CNRS 8161 Institut de Biologie de Lille 1, Lille Cedex, France.
authors: Vianney
authors: V
authors: Souplet
authors: Clément
authors: C
authors: Roux
authors: Oleg
authors: O
authors: Melnyk
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D006801:Humans; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011108:Polymers / Q000494:pharmacology*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology; D000098826:Bisphenol A Compounds; D000098827:Polycarbonates
chemical_list: D001559:Benzhydryl Compounds; D019151:Peptide Library; D010455:Peptides; D011108:Polymers; D012822:Silicon Dioxide; C028237:bisphenol-A-polycarbonate; D000098826:Bisphenol A Compounds; D000098827:Polycarbonates