title: Functional properties of hemocyanin from Oncomelania hupensis, the intermediate host of Schistosoma japonicum.
abstract: The gastropod mollusc, Oncomelania hupensis is a unique intermediate host for the human parasite Schistosoma japonicum. It is a primary factor for the epidemic of schistosomiasis and its distribution is consistent with the epidemic area of schistosomiasis. Here we report the functional properties of hemocyanin of O. hupensis (OhH), a copper-containing respiratory protein which was isolated from its hemolymph and purified by ammonium sulfate fractionation and ultracentrifugation. We identified the protein characters including UV absorption at 340 nm, copper content and quaternary structure. Furthermore, by induction of phenoloxidase and enzyme-linked immunosorbent assay we show that OhH exhibited o-diphenoloxidase activity after limited proteolysis, and shared carbohydrate epitopes with glycoconjugates of S. japonicum.
authors: Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Gannan Normal University, Ganzhou 341000, People's Republic of China.
authors: Daoyi
authors: D
authors: Guo
authors: Yun
authors: Y
authors: Zhang
authors: Dan
authors: D
authors: Zeng
authors: Hua
authors: H
authors: Wang
authors: Xun
authors: X
authors: Li
authors: Yongdong
authors: Y
authors: Li
authors: Xiaolin
authors: X
authors: Fan
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D003300:Copper / Q000032:analysis; D049851:Gastropoda / Q000737:chemistry / Q000469:parasitology / Q000502:physiology*; D006433:Hemocyanins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D006458:Hemolymph / Q000737:chemistry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D018716:Molecular Mimicry; D014442:Monophenol Monooxygenase / Q000032:analysis; D012549:Schistosoma japonicum / Q000502:physiology*; D012554:Schistosomiasis japonica / Q000635:transmission
chemical_list: D002241:Carbohydrates; D007106:Immune Sera; D003300:Copper; D006433:Hemocyanins; D014442:Monophenol Monooxygenase