Micromonospora marina sp. nov., isolated from sea sand.
Two actinomycete strains, JSM1-1(T) and JSM1-3, were isolated from sea sand collected in Thailand. Their taxonomic position was determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the genus Micromonospora, i.e. the presence of meso-diaminopimelic acid and N-glycolyl muramic acid in the peptidoglycan, whole cell sugar pattern D, phospholipids type II, and cellular fatty acid type 3b. Phylogenetic analysis of 16S rRNA gene sequences revealed a close relationship between strains JSM1-1(T) and JSM1-3 (99.8 %), and between JSM1-1(T) and Micromonospora aurantiaca JCM 10878(T) (99.3 %), Micromonospora chalcea JCM 3031(T) (99.0 %), and Micromonospora coxensis JCM 13248 (T) (99.0 %). However, strains JSM1-1(T) and JSM1-3 could be clearly distinguished from these type strains by a low DNA-DNA relatedness and by phenotypic differences. On the basis of the data presented, a new species, Micromonospora marina sp. nov., is proposed. The type strain is JSM1-1(T) (=JCM 12870(T) =PCU 269(T) =TISTR 1566(T)).
Tanasupawat
Somboon
S
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Jongrungruangchok
Suchada
S
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Kudo
Takuji
T
Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013785:Thailand
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S