Improved resolution in the acidic and basic region of 2-DE of insect antennae proteins using hydroxyethyl disulfide.
Olfaction is essential for processing chemical signals in insects, but characterizing the proteins implicated in this process has proved challenging. We optimized 2-DE gel resolution of insect proteins by using a buffer containing two reducing agents, DTT and hydroxyethyl disulfide. This buffer clearly improved resolution and decreased spot streaking and spot trains of 2-DE in comparison to DTT alone. We described for the first time that the buffer with DTT and hydroxyethyl disulfide further to reducing streaking in the basic part of the gel eliminates false spots in the acidic gel regions that appeared when only DTT was used as reducing agent.
Acín
Patricia
P
Department of Biological Chemistry and Molecular Modeling, IQAC-CSIC, Jordi Girona, Barcelona, Spain.
Rayó
Josep
J
Guerrero
Angel
A
Quero
Carmen
C
D000818:Animals; D004220:Disulfides / Q000737:chemistry*; D004229:Dithiothreitol / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D000431:Ethanol / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D019476:Insect Proteins / Q000302:isolation & purification*; D007915:Lepidoptera / Q000737:chemistry*; D008297:Male
D004220:Disulfides; D019476:Insect Proteins; D000431:Ethanol; C031319:2-hydroxyethyl disulfide; D004229:Dithiothreitol