Screening of polycyclic polyprenylated acylphloroglucinols from Garcinia species using precursor ion discovery (PID) scan and ultra performance liquid chromatography electrospray ionization Q-TOF tandem mass spectrometry.
A strategy was newly developed to rapidly screen polycyclic polyprenylated acyl-phloroglucinols (PPAPs) from the plant matrices of nine Garcinia species using ultra-performance liquid chromatography (UPLC) coupled with comprehensive mass spectrometric approaches including precursor ion discovery (PID) and tandem mass (MS/MS) scans. The PPAPs share the same diagnostic product ion at m/z 177.02 in positive MS/MS scan, which may be increased as the base peak by ramping the cone voltage from 45 to 100 V. With this ramping cone voltage PID scan, it is feasible to selectively screen the PPAPs from 29 samples of nine Garcinia species. This approach has proven to be a powerful, highly selective, and sensitive tool for rapid screening and detection of nontargeted components in natural products before the purification and structural elucidation process.
Zhou
Yan
Y
Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, China.
Huang
Sheng-Xiong
SX
Song
Jing-Zheng
JZ
Qiao
Chun-Feng
CF
Li
Song-Lin
SL
Han
Quan-Bin
QB
Xu
Hong-Xi
HX
D001577:Benzophenones / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029760:Garcinia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000737:chemistry*; D054876:Prenylation; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013729:Terpenes / Q000737:chemistry
D001577:Benzophenones; D010936:Plant Extracts; D011083:Polycyclic Compounds; D013729:Terpenes