Oceanibaculum pacificum sp. nov., isolated from hydrothermal field sediment of the south-west Pacific Ocean.
A taxonomic study was carried out on strain LMC2up-L3(T), which was isolated from a polycyclic aromatic hydrocarbon-degrading consortium enriched with a sediment sample collected from a hydrothermal field of the south-west Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LMC2up-L3(T) belonged to the genus Oceanibaculum, with the highest sequence similarity of 98.4 % to Oceanibaculum indicum P24(T); similarity to other strains was below 93.1 %. DNA-DNA hybridization between strain LMC2up-L3(T) and O. indicum P24(T) was 31 %. rep-PCR fingerprints also differentiated strain LMC2up-L3(T) from O. indicum P24(T). The G+C content of the chromosomal DNA was 67.7 mol%. The principal fatty acids were C(16 : 1) (17.8 %), C(16 : 0) (21.2 %), C(18 : 1)omega7c (23.6 %), C(18 : 0) (4.1 %), C(18 : 1) 2-OH (4.5 %) and C(19 : 0) cyclo omega8c (17.4 %). The combined genotypic and phenotypic data show that strain LMC2up-L3(T) represents a novel species of the genus Oceanibaculum, for which the name Oceanibaculum pacificum sp. nov. is proposed, with the type strain LMC2up-L3(T) (=CCTCC AB 209059(T) =LMG 24859(T) =MCCC 1A02656(T)). An emended description of the genus Oceanibaculum is also provided.
Dong
Chunming
C
State Key Laboratory of Agricultural Microbiology, National Engineering Research Centre of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, 430070 Wuhan, PR China.
Lai
Qiliang
Q
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, 361005 Xiamen, PR China.
Chen
Liang
L
State Key Laboratory of Agricultural Microbiology, National Engineering Research Centre of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, 430070 Wuhan, PR China.
Sun
Fengqin
F
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, 361005 Xiamen, PR China.
Shao
Zongze
Z
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, 361005 Xiamen, PR China.
Yu
Ziniu
Z
State Key Laboratory of Agricultural Microbiology, National Engineering Research Centre of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, 430070 Wuhan, PR China.
D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S