The effect of electrical stimulation on the differentiation of hESCs adhered onto fibronectin-coated gold nanoparticles.
To encourage stem cell differentiation, gold nanoparticles (20 nm) were used to deliver electrical stimulation to human embryonic stem cells (hESCs) in vitro. Nano-structured gold nanoparticles were designed by coating the surface of culture dishes with gold nanoparticles using a layer-by-layer (LBL) system. In this method, gold nanoparticles were continuously coated onto dishes by SEM analysis. Evaluation of gene modified hESCs that were subsequently attached onto fibronectin-coated gold nanoparticles revealed that the un-differentiation marker, Oct-4, was no longer present following electrical stimulation. In addition, the osteogenic markers of collagen type I and Cbfa1 increased in response to electrical stimulation, while those of hESCs were not observed without electrical stimulation.
Woo
Dae G
DG
College of Medicine, Pochon CHA University, CHA Stem Cell Institute 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, South Korea.
Shim
Myung-Sun
MS
Park
Ji S
JS
Yang
Han N
HN
Lee
Dong-Ryul
DR
Park
Keun-Hong
KH
D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004558:Electric Stimulation / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D005353:Fibronectins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D010012:Osteogenesis / Q000502:physiology / Q000528:radiation effects
D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D006046:Gold