Antiproliferative effects of withanolides from Withania adpressa.
Extracts of Withania adpressa Coss. (Solanaceae), a medicinal plant endemic to Moroccan Sahara, were tested for their cytotoxicity towards a panel of cancer cell lines (Hep2, HT29, RD, Vero and MDCK), using the (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) [MTT assay, Sigma-Aldrich]. The bioassay-guided fractionation of this plant extracts results a novel withanolide 14alpha,15alpha,17beta ,20beta-tetrahydroxy-1-oxo-(22R)-witha-2,5,24-trienolide and the already identified withanolides F and J extract, semi-purified fractions and pure compounds exhibits potent cytotoxicity against human cancer cell lines tested, in dose-dependent manner. Morphological features of treated Hep2 cells with the novel withanolide and characteristic DNA fragmentation revealed that the cytotoxicity was due to induction of apoptosis. Taken together, the results suggest that withanolides from W. adpressa Coss. hold potential as antiproliferative agents.
Abdeljebbar
Lalla Hasna
LH
Laboratoire de Biochimie et Immunologie, Faculté des Sciences, Université Mohammed V-Agdal, Rabat, Maroc.
Benjouad
Abdelaziz
A
Morjani
Hamid
H
Merghoub
Nawal
N
El Haddar
Saoussan
S
Humam
Munir
M
Christen
Philippe
P
Hostettmann
Kurt
K
Bekkouche
Khalid
K
Amzazi
Saaïd
S
D000972:Antineoplastic Agents, Phytogenic; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D032341:Withania / Q000737:chemistry*; D054358:Withanolides / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; D054358:Withanolides; C022616:thiazolyl blue