Strenuous exercise increases late outgrowth endothelial cells in healthy subjects.
Endothelial progenitor cells (EPCs) and late outgrowth endothelial cells (OECs) seem to play an important role in vessel formation. While EPCs seem to exert their function mainly through a paracrine effect, the OECs can develop into mature endothelial cells and form tubular structures. Exercise is known to increase angiogenic factors that can mobilize EPCs; however, the effect on OECs is not known. We investigated the response to a single session of strenuous exercise on OECs, vascular endothelial growth factor (VEGF) and inflammatory cell levels in the healthy. Eleven healthy subjects performed 1 h of spinning exercise. Blood samples were collected at 1, 6, 24 and 48 h post-exercise for cell culture and biochemical analysis. OEC colonies doubled one hour after the spinning session (baseline 4.5 +/- 4.3 vs. 9.0 +/- 3.7, P < 0.05). Serum VEGF increased from 194 +/- 107 pg/ml at baseline to 224 +/- 111 pg/ml after 1 h, p = ns and neutrophilic granulocytes increased from 3.73 +/- 1.38 at baseline to 9.08 +/- 10.5 at 1 h (P < 0.01). The increased levels of OECs, VEGF and neutrophilic granulocytes declined gradually at the following time points. VEGF levels and neutrophilic granulocytes were highly correlated to OEC levels, r = 0.903 (VEGF) and r = 0.85 (neutrophilic granulocytes), respectively. Strenuous physical activity increases OEC colonies and is correlated to serum VEGF and neutrophilic granulocytes levels. An acute exercise-induced inflammatory response might be responsible for the VEGF release and subsequent increase of OECs. The clinical importance of these findings remains to be elucidated.
Thorell
Daniel
D
Department of Emergency and Cardiovascular Medicine, Sahlgrenska University Hospital/Ostra, Institute of Medicine, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Borjesson
Mats
M
Larsson
Pia
P
Ulfhammer
Erik
E
Karlsson
Lena
L
DuttaRoy
Smita
S
D000328:Adult; D002097:C-Reactive Protein / Q000032:analysis; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005260:Female; D006262:Health; D006801:Humans; D008297:Male; D008875:Middle Aged; D005082:Physical Exertion / Q000502:physiology*; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D055815:Young Adult
C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D002097:C-Reactive Protein