[In vitro interferon-gamma induction in whole blood samples is a test for tuberculous infection in children and adolescents].
By applying the interferon-gamma (IFN-gamma) induction technique in the whole blood samples exposed to short-term (22-24-hour) incubation in the presence of Mycobacterium tuberculosis antigens--PPD tuberculin and specific recombinant ESAT-6 lacking in the cells of vaccine BCG and other non-tuberculous mycobacteria, the authors studied the groups of children and adolescents with a negative Mantoux test (n = 31), with postvaccine BCG allergy (n = 40), as well as patients with primary tuberculous infection (n = 84) and those with pulmonary tuberculosis (n = 44). Patients with primary tuberculous infection and a high sensitivity (94%) and a high specificity (97%) may be differentiated from children and adolescents with postvaccinal allergy when the recombinant ESAT-6 antigen and the critical IFN-gamma level (greater than 70 pg/ml) detectable in the plasma samples after incubation with the antigen. It has been also shown that in adolescents with local forms of pulmonary tuberculosis specific IFN-gamma induction may be suppressed in number of cases, which is ascribed to decreased specific immunity.
Mordovskaia
L I
LI
Vladimirskiĭ
M A
MA
Aksenova
V A
VA
Efremov
E E
EE
Ignashenkova
G I
GI
Vlasik
T N
TN
D000293:Adolescent; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007111:Immunity, Cellular; D007223:Infant; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification; D011379:Prognosis; D014374:Tuberculin Test; D014376:Tuberculosis / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D032581:Tuberculosis Vaccines / Q000276:immunology
D000942:Antigens, Bacterial; D032581:Tuberculosis Vaccines; D007371:Interferon-gamma