title: [Investigation on degradation process of collagens during manufacturing procedure of E'jiao].
abstract: OBJECTIVE
  To investigate the degradation process of collagens and identify the key unit operation during manufacturing process of E'jiao.
  
  
  METHODS
  Samples in different unit operations were withdrawn, and their amino acid compositions and the molecular weight ranges were determined. The peptide composition was analyzed by high-performance liquid chromatography/mass spectrometry.
  
  
  RESULTS
  The content of sample during atmospheric condensation unit increased by 16.8% compared to the thermal extraction unit. Gel filtration chromatographic analysis indicated that the degradation process of collagen primarily occurred during the atmospheric condensation unit. The peptides in samples mainly resulted from the degradation of collagens and cytoskeleton proteins such as tubulin, actinin, and so on. The relative abundance of degraded collagens increased with the decrease of no-collagen proteins.
  
  
  CONCLUSIONS
  Collagen degradation mainly occurred during the atmospheric condensation unit, which was the key process affecting the composition of E-jiao.
authors: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.
authors: Guifeng
authors: G
authors: Zhang
authors: Tao
authors: T
authors: Liu
authors: Qian
authors: Q
authors: Wang
authors: Jian
authors: J
authors: Luo
authors: Ru
authors: R
authors: Xie
authors: Yufeng
authors: Y
authors: Qin
authors: Jinhua
authors: J
authors: You
authors: Shousheng
authors: S
authors: Tian
authors: Zhiguo
authors: Z
authors: Su
mesh_terms: D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*
chemical_list: D010455:Peptides; D003094:Collagen