[Effects of fusion gene of ubiquitin and porcine reproductive and respiratory syndrome virus M gene on the immune response in inoculated mice].
OBJECTIVE
To evaluate the effects of the fusion gene of ubiquitin (Ub) and Porcine Reproductive and Respiratory Syndrome virus (PRRSV) M gene on the immune response in inoculated mice.


METHODS
Mouse Ub gene and PRRSV M gene were amplified by RT-PCR from BALB/c mice spleen cells and PRRSV Ch-1a strain, respectively, and the M and Ub gene (U-M) was fused by SOE PCR. Therefore, pVAX1-U-M and pVAX1-M recombinant plasmid were constructed for eukaryotic expression.


RESULTS
The fusion U-M and M protein expressions were verified in transfected BHK-21 cells by indirect fluorescence assay. Furthermore, both pVAX1-M and pVAX1-U-M induced specific humoral and cellar immune responses against PRRSV in the recombinant plasmid injected mice. However, pVAX1-U-M was able to induce higher level of T cell response then that of pVAX1-M (P<0.05), but lower level of antibody (P<0.05).


CONCLUSIONS
Expression of U-M fusion gene had ability to enhance specific T cell response against PRRSV, but no effect on stimulation of humoral response in inoculated mice.
Zhang
Weijun
W
State Key Laboratory of Veterinary Biotechnology, The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. zwj-216@163.com
Tong
Tiegang
T
Bai
Yu
Y
Wang
Qun
Q
Xu
Shulan
S
Sun
Qingge
Q
Tian
Ye
Y
Yang
Tao
T
Tong
Guangzhi
G
Wu
Donglai
D
D000818:Animals; D015870:Gene Expression; D050939:Gene Fusion / Q000276:immunology*; D055633:Immune System Phenomena / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000494:pharmacology*; D013552:Swine; D014162:Transfection; D025801:Ubiquitin / Q000235:genetics* / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage
D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; D014763:Viral Matrix Proteins; D014765:Viral Vaccines