title: Histamine modulates the cellular stress response in yeast.
abstract: The cellular stress response is a universal protective reaction to adverse environmental or microenvironmental conditions, such as heat and drugs, associated in part with the highly conserved heat shock proteins (HSPs). Histamine is a key inflammatory mediator derived from L: -histidine that governs vital cellular processes beyond inflammation, while recent evidence implies additional actions in both prokaryotes and eukaryotes. This study explored the possible role of histamine in the heat shock response in yeast, an established experimental model for the pharmacological investigation of the cellular stress response. The response was evaluated by determining growth and viability of post-logarithmic phase grown yeast cultures after heat shock at 53 degrees C for 30 min. Thermal preconditioning at 37 degrees C for 2 h served as a positive control. The effect of histamine was investigated following long-term administration through the post-logarithmic phase of growth or short-term administration for 2 h prior to heat shock. Short-term treatment with 1 mM histamine resulted in de novo protein synthesis-dependent acquisition of thermotolerance, while lower doses or long-term administration of histamine failed to induce the heat-resistant phenotype. Preliminary investigation of HSP104, HSP70 and HSP60 expression by western blotting showed an increase of these proteins after thermal preconditioning. However, a differential HSP and tubulin expression appeared to underlie the response of yeast cells to histamine. In conclusion, histamine was capable of inducing the adaptive phenotype, while the contribution of HSPs and tubulin and the potential implications remain largely elusive.
authors: Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece.
authors: Basil
authors: B
authors: Delitheos
authors: Konstantinos
authors: K
authors: Papamichael
authors: Ekaterini
authors: E
authors: Tiligada
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D018834:Chaperonin 60 / Q000378:metabolism; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006632:Histamine / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018834:Chaperonin 60; C504495:HSP60 protein, S cerevisiae; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D020011:Protective Agents; C090869:SSA3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014404:Tubulin; C070972:HsP104 protein, S cerevisiae; D006632:Histamine