[Effect of Flammulina velutipes polysaccharides on production of cytokines by murine immunocytes and serum levels of cytokines in tumor-bearing mice].
OBJECTIVE
To study the effect of Flammulina velutipes polysaccharides (FVP) on the production of tumor necrosis factor alpha (TNF-alpha), interferon-gamma (INF-gamma) and interleukin 2 (IL-2) by murine immunocytes.


METHODS
The cell's metabolic activity was determined with methylthiazolyl tetrazolium (MTT) colorimetry assay and the amounts of TNF-alpha, INF-gamma and IL-2 were measured by ELISA.


RESULTS
FVP (200, 100, 50 microg/mL) could promote the metabolic activity of murine splenocytes and peritoneal exudate cells (PEC) and increase the amounts of TNF-alpha, INF-gamma and IL-2 in the supernatants of splenocyte cultures, and the amount of TNF-alpha in PEC cultures, with the most marked increase on TNF-alpha level. FVP (100, 50, 25 mg/kg) could raise the serum levels of TNF-alpha and INF-gamma in S180 tumor-bearing mice.


CONCLUSIONS
FVP may regulate murine immune function through promoting the production of TNF-alpha, INF-gamma and IL-2.
Chang
Hua-Lei
HL
Institute of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. chlshe@sohu.com
Lei
Lin-Sheng
LS
Yu
Chuan-Lin
CL
Zhu
Zheng-Guang
ZG
Chen
Na-Na
NN
Wu
Shu-Guang
SG
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood; D007376:Interleukin-2 / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D012510:Sarcoma 180 / Q000097:blood* / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000097:blood
D016207:Cytokines; D007376:Interleukin-2; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma