title: Fine mapping and marker development for the crossability gene SKr on chromosome 5BS of hexaploid wheat (Triticum aestivum L.).
abstract: Most elite wheat varieties cannot be crossed with related species thereby restricting greatly the germplasm that can be used for alien introgression in breeding programs. Inhibition to crossability is controlled genetically and a number of QTL have been identified to date, including the major gene Kr1 on 5BL and SKr, a strong QTL affecting crossability between wheat and rye on chromosome 5BS. In this study, we used a recombinant SSD population originating from a cross between the poorly crossable cultivar Courtot (Ct) and the crossable line MP98 to characterize the major dominant effect of SKr and map the gene at the distal end of the chromosome near the 5B homeologous GSP locus. Colinearity with barley and rice was used to saturate the SKr region with new markers and establish orthologous relationships with a 54-kb region on rice chromosome 12. In total, five markers were mapped within a genetic interval of 0.3 cM and 400 kb of BAC contigs were established on both sides of the gene to lay the foundation for map-based cloning of SKr. Two SSR markers completely linked to SKr were used to evaluate a collection of crossable wheat progenies originating from primary triticale breeding programs. The results confirm the major effect of SKr on crossability and the usefulness of the two markers for the efficient introgression of crossability in elite wheat varieties.
authors: Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal, Unité Mixte de Recherche 1095, Genetics Diversity and Ecophysiology of Cereals, 63100 Clermont-Ferrand, France.
authors: Walid
authors: W
authors: Alfares
authors: Annaig
authors: A
authors: Bouguennec
authors: François
authors: F
authors: Balfourier
authors: Georges
authors: G
authors: Gay
authors: Hélène
authors: H
authors: Bergès
authors: Sonia
authors: S
authors: Vautrin
authors: Pierre
authors: P
authors: Sourdille
authors: Michel
authors: M
authors: Bernard
authors: Catherine
authors: C
authors: Feuillet
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D020451:Contig Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D011110:Polymorphism, Genetic; D011123:Polyploidy; D017422:Sequence Analysis, DNA; D026801:Synteny; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins