DNA vaccines expressing retrovirus-like particles are efficient immunogens to induce neutralizing antibodies.
We aimed at improving DNA vaccination efficiency for inducing neutralizing antibodies. We used plasmids encoding Gag of MLV and envelope proteins of VSV or WNV. Upon in vivo injection, they generate retrovirus-derived VLPs pseudotyped with these envelopes expressed in their wild-type conformation. We show that these plasmo-retroVLPs induce potent humoral responses, the efficacy of which could be improved by co-administration of DNA encoding adjuvant cytokines. Antibodies against VSV or WNV were detected earlier than with plasmids not generating VLPs, and had higher neutralizing activities. These results highlight the potential of this approach for vaccination strategies aiming at neutralizing antibody induction.
Bellier
Bertrand
B
UPMC Univ Paris 06, UMR7211, F-75013 Paris, France.
Huret
Christophe
C
Miyalou
Mathilde
M
Desjardins
Delphine
D
Frenkiel
Marie-Pascale
MP
Despres
Philippe
P
Tangy
Fréderic
F
Dalba
Charlotte
C
Klatzmann
David
D
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D005260:Female; D015686:Gene Products, env / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009500:Neutralization Tests; D012190:Retroviridae / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D018116:Vesiculovirus / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014902:West Nile virus / Q000276:immunology
D000914:Antibodies, Viral; C028391:G protein, vesicular stomatitis virus; D015686:Gene Products, env; D008562:Membrane Glycoproteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines