title: Cryobacterium roopkundense sp. nov., a psychrophilic bacterium isolated from glacial soil.
abstract: Strain RuGl7(T) was isolated from a soil sample collected at the periphery of the glacial Lake Roopkund in the Himalayan mountain range, India. Cells of RuGl7(T) were Gram-positive, aerobic, rod-shaped, motile and grew optimally between 15 and 18 degrees C. Cells of RuGl7(T) contained 2,4-diaminobutyric acid in the cell-wall peptidoglycan and the major menaquinones were MK-10, MK-11 and MK-12. The polar lipids present were diphosphatidylglycerol and phosphatidylglycerol and an unknown lipid and the major fatty acid was anteiso-C(15 : 0). Based on the above characteristics, strain RuGl7(T) was assigned to the genus Cryobacterium. Strain RuGl7(T) shared a 16S rRNA gene sequence similarity of 97.0 and 99.0 % with Cryobacterium psychrotolerans JCM 13925( T) and Cryobacterium psychrophilum JCM 1463(T), respectively. However, DNA-DNA relatedness values between strain RuGl7(T) and C. psychrotolerans and C. psychrophilum were 28 and 23 %, respectively. Furthermore, strain RuGl7(T) exhibited several phenotypic and genotypic differences when compared with C. psychrotolerans , C. psychrophilum and Cryobacterium mesophilum. Based on these differentiating characteristics, strain RuGl7(T) was identified as a novel species of the genus Cryobacterium for which the name Cryobacterium roopkundense sp. nov. is proposed. The type strain is RuGl7( T) (=DSM 21065(T)=JCM 15131(T)).
authors: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors: G S N
authors: GSN
authors: Reddy
authors: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors: Suman
authors: S
authors: Pradhan
authors: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors: Ruth
authors: R
authors: Manorama
authors: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
authors: S
authors: S
authors: Shivaji
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D003080:Cold Temperature; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D046448:Ice Cover / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S