title: Aberrant processing of plasma vitronectin and high-molecular-weight kininogen precedes the onset of preeclampsia.
abstract: To date, there is no reliable test to identify women in early pregnancy at risk of developing preeclampsia. Difference gel electrophoresis (DIGE) identified the plasma proteins vitronectin (VN) and high-molecular-weight kininogen (HK) in association with preeclampsia. In a longitudinal proteomics study, the plasma of preeclamptic patients (n = 6) was compared to healthy control participants (n = 6) before the onset of preeclampsia (week 20) and at the time of presentation with clinical disease (weeks 33-36). The 75-kd single-chain VN molecule increased 1.6- to 1.9-fold in preeclampsia, whereas the 65-kd moiety of the 2-chain VN molecule decreased 1.5- to 1.7-fold compared to healthy controls (P < .05). Immunoblots revealed differences in proteolytic processing of VN and/or HK in women who develop preeclampsia or preeclampsia further complicated by small-for-gestational-age. Vitronectin and HK may prove to be useful as early markers of fibrinolytic activity and neutrophil activation, which are known to be associated with preeclampsia.
authors: School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand. m.blumenstein@auckland.ac.nz
authors: Marion
authors: M
authors: Blumenstein
authors: Roneel
authors: R
authors: Prakash
authors: Garth J S
authors: GJ
authors: Cooper
authors: Robyn A
authors: RA
authors: North
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D019679:Kininogen, High-Molecular-Weight / Q000097:blood* / Q000737:chemistry; D008137:Longitudinal Studies; D008970:Molecular Weight; D011225:Pre-Eclampsia / Q000097:blood* / Q000276:immunology; D011247:Pregnancy; D011256:Pregnancy Outcome; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011499:Protein Processing, Post-Translational; D012720:Severity of Illness Index; D053719:Tandem Mass Spectrometry; D019096:Vitronectin / Q000097:blood* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D019679:Kininogen, High-Molecular-Weight; D019096:Vitronectin