title: Peritoneal resorption capacity for the inflammatory mediators in acute experimental Staphylococcus aureus peritonitis.
abstract: BACKGROUND
  Elevations in both endotoxin and interleukin-6 (IL-6) concentrations in peritoneal exudates are a thousand times higher than their respective concentrations in the peripheral blood in patients with gram-positive or gram-negative peritonitis. We aimed in this study to evaluate the resorption capacity of the peritoneum for endotoxin and IL-6 in a model of bacterial (gram-positive) peritonitis.
  
  
  METHODS
  Intraperitoneal (i.p.) injection of mucin-pretreated staphylococci in phosphate buffered saline (PBS) or of PBS alone was performed in 93 male Wistar rats. Studies of resorption were undertaken at time points of 4 hours (h), 8h, 12h and 24h. Endotoxin was intraperitoneally injected in 44 rats and IL-6 in 49 rats. After 0, 5, 10, 15, 30 and 60 minutes (min), blood was sampled. Endotoxin and IL-6 were measured using the limulus-amoebocyte-lysate (LAL) test and ELISA technique, respectively.
  
  
  RESULTS
  No endotoxin or IL-6 was measured in the blood of controls. Plasma endotoxin and IL-6 levels were significantly high in the peritonitis groups. There was no further increase in endotoxin plasma levels after i.p. injection of endotoxin. Following i.p. injection of IL-6, there was an increase in IL-6 level over the time of sampling in the peripheral blood at 4h of peritonitis.
  
  
  CONCLUSIONS
  There was a clear reduction in peritoneal resorption of endotoxin and IL-6 in this acute model of gram-positive peritonitis.
authors: Department of Surgery, UAE University, Al-Ain, United Arab Emirates. ftorab@uaeu.ac.ae
authors: Fawaz Chikh
authors: FC
authors: Torab
authors: Fikri M
authors: FM
authors: Abu-Zidan
authors: Suhail
authors: S
authors: Al-Salam
authors: Dieter
authors: D
authors: Berger
authors: Frank James
authors: FJ
authors: Branicki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004731:Endotoxins / Q000493:pharmacokinetics*; D007274:Injections, Intraperitoneal; D015850:Interleukin-6 / Q000493:pharmacokinetics*; D008297:Male; D010537:Peritoneum / Q000378:metabolism*; D010538:Peritonitis / Q000097:blood* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000097:blood* / Q000503:physiopathology
chemical_list: D004731:Endotoxins; D015850:Interleukin-6