title: Differential expression of cell surface proteins in human bone marrow mesenchymal stem cells cultured with or without basic fibroblast growth factor containing medium.
abstract: Mesenchymal stem cells (MSCs) are multipotent cells, which have the capability to differentiate into various mesenchymal tissues such as bone, cartilage, fat, tendon, muscle, and marrow stroma. However, they lose the capability of multi-lineage differentiation after several passages. It is known that basic fibroblast growth factor (bFGF) increases growth rate, differentiation potential, and morphological changes of MSCs in vitro. In this report, we have used 2-DE coupled to MS to identify differentially expressed proteins at the cell membrane level in MSCs growing in bFGF containing medium. The cell surface proteins isolated by the biotin-avidin affinity column were separated by 2-DE in triplicate experiments. A total of 15 differentially expressed proteins were identified by quadrupole-time of flight tandem MS. Nine of the proteins were upregulated and six proteins were downregulated in the MSCs cultured with bFGF containing medium. The expression level of three actin-related proteins, F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2, was confirmed by Western blot analysis. The results indicate that the expression levels of F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2 are important in bFGF-induced morphological change of MSCs.
authors: Mass Spectrometry Research Center, Korea Basic Science Institute, Daejeon, Republic of Korea.
authors: Sang Kwang
authors: SK
authors: Lee
authors: Yongtae
authors: Y
authors: Kim
authors: Sung-Soo
authors: SS
authors: Kim
authors: Jeong Hwa
authors: JH
authors: Lee
authors: Kun
authors: K
authors: Cho
authors: Sang Sook
authors: SS
authors: Lee
authors: Zee-Won
authors: ZW
authors: Lee
authors: Kyung-Hoon
authors: KH
authors: Kwon
authors: Young Hye
authors: YH
authors: Kim
authors: Haeyoung
authors: H
authors: Suh-Kim
authors: Jong Shin
authors: JS
authors: Yoo
authors: Young Mok
authors: YM
authors: Park
mesh_terms: D000199:Actins / Q000378:metabolism; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000096:biosynthesis*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D000199:Actins; D003470:Culture Media; D008565:Membrane Proteins; D011506:Proteins; D016222:Fibroblast Growth Factor 2