title: Mesenchymal stem cells and inorganic bovine bone mineral in sinus augmentation: comparison with augmentation by autologous bone in adult sheep.
abstract: Our aim was to compare the osteogenic potential of mononuclear cells harvested from the iliac crest combined with bovine bone mineral (BBM) (experimental group) with that of autogenous cancellous bone alone (control group). We studied bilateral augmentations of the sinus floor in 6 adult sheep. BBM and mononuclear cells (MNC) were mixed and placed into one side and autogenous bone in the other side. Animals were killed after 8 and 16 weeks. Sites of augmentation were analysed radiographically and histologically. The mean (SD) augmentation volume was 3.0 (1.0) cm(3) and 2.7 (0.3) cm(3) after 8 and 16 weeks in the test group, and 2.8 (0.3) cm(3) (8 weeks) and 2.8 (1.2) cm(3) (16 weeks) in the control group, respectively. After 8 weeks, histomorphometric analysis showed 24 (3)% BBM, and 19 (11)% of newly formed bone in the test group. The control group had 20 (13%) of newly formed bone. Specimens after 16 weeks showed 29 (12%) of newly formed bone and 19 (3%) BBM in the test group. The amount of newly formed bone in the control group was 16 (6%). The results show that mononuclear cells, including mesenchymal stem cells, in combination with BBM as the biomaterial, have the potential to form bone.
authors: Universitätsklinik für Zahn-, Mund- und Kieferheilkunde, Abteilung Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hugstetter Str. 55, D-79106 Freiburg, Germany.
authors: Ralf
authors: R
authors: Gutwald
authors: Jörg
authors: J
authors: Haberstroh
authors: Jens
authors: J
authors: Kuschnierz
authors: Carola
authors: C
authors: Kister
authors: Dominikus A
authors: DA
authors: Lysek
authors: Michele
authors: M
authors: Maglione
authors: S P
authors: SP
authors: Xavier
authors: Toshiyuki
authors: T
authors: Oshima
authors: Rainer
authors: R
authors: Schmelzeisen
authors: Sebastian
authors: S
authors: Sauerbier
mesh_terms: D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001857:Bone Matrix / Q000473:pathology / Q000637:transplantation*; D016723:Bone Remodeling / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use*; D002417:Cattle; D002452:Cell Count; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006253:Haversian System / Q000473:pathology; D008437:Maxilla / Q000473:pathology / Q000601:surgery; D008443:Maxillary Sinus / Q000473:pathology / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008903:Minerals / Q000627:therapeutic use*; D010010:Osteoclasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D012756:Sheep; D013997:Time Factors; D014057:Tomography, X-Ray Computed; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008903:Minerals; C007740:fluorexon