title: Early antiallergic inflammatory effects of Rhus verniciflua Stokes on human mast cells.
abstract: Rhus verniciflua Stokes (RVS) is a traditional medicine used in Korea, Japan and China to treat various diseases including catharsis, diaphoretic gastritis and stomach cancer. However, the effects of RVS on allergic inflammatory diseases are unknown to date. This study showed the antiallergic inflammatory effects of RVS on human mast cells (HMC-1) which were stimulated by phorbol myristate acetate (PMA) and calcium ionophore A23187. RVS inhibited the expressions of TNF-alpha, IL-6 and IL-8 that were stimulated by treatment with both PMA and A23187. Among the mitogen-activated protein kinases (MAPKs), extracts of RVS suppressed the phosphorylation of ERK and p38, whereas RVS increased the phosphorylation of JNK in HMC-1. Consistent with the regulation of MAPKs, it was found that RVS inhibited the nuclear translocation of nuclear factor (NF)-kappaB via inhibition of the phosphorylation of IkappaB-alpha, which are important processes in controlling inflammatory responses. Taken together, these results suggest that RVS modulates the expressions of signal molecules related to allergic inflammatory responses mainly through the ERK signaling pathway, suggesting that RVS could be used as a treatment for mast cell-derived allergic inflammatory diseases.
authors: Department of Preventive Medicine, College of Oriental Medicine, Kyunghee University, Seoul, Republic of Korea.
authors: Myung Hee
authors: MH
authors: Hong
authors: Jeong-Hyun
authors: JH
authors: Kim
authors: Sea Yun
authors: SY
authors: Lee
authors: Ho Yeon
authors: HY
authors: Go
authors: Ji Hye
authors: JH
authors: Kim
authors: Yong-Cheol
authors: YC
authors: Shin
authors: Sung-Hoon
authors: SH
authors: Kim
authors: Seong-Gyu
authors: SG
authors: Ko
mesh_terms: D018926:Anti-Allergic Agents / Q000494:pharmacology*; D000001:Calcimycin; D002460:Cell Line; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D029045:Rhus / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D020928:Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate