title: Comprehensive proteomic analysis of human endometrial fluid aspirate.
abstract: The endometrial fluid is a noninvasive sample which contains numerous secreted proteins representative of endometrial function and reflects the state of the endometrium. In this study, we describe, for the first time, a comprehensive catalogue of proteins of the endometrial fluid during the secretory phase of the menstrual cycle. To achieve this objective, three different but complementary strategies were used: First, in-solution digestion followed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS); second, protein separation by denaturing one-dimensional electrophoresis (SDS-PAGE) followed by HPLC-MS/MS analysis. Finally, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) followed by MALDI-TOF/TOF analysis. The combination of the three strategies led to the successful identification of 803 different proteins in the International Protein Index (IPI) human database (v3.48). An extensive description of the endometrial fluid proteome will help provide the basis for a better understanding of a number of diseases and processes, including endometriosis, endometrial cancer and embryo implantation. We believe that the thorough catalogue of proteins presented here can serve as a valuable reference for the study of embryo implantation and for future biomarker discovery involved in pathologic alterations of endometrial function.
authors: Proteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed, Technology Park of Bizkaia, Derio, Spain.
authors: Juan
authors: J
authors: Casado-Vela
authors: Eva
authors: E
authors: Rodriguez-Suarez
authors: Ibon
authors: I
authors: Iloro
authors: Amagoia
authors: A
authors: Ametzazurra
authors: Nere
authors: N
authors: Alkorta
authors: Juan Antonio
authors: JA
authors: García-Velasco
authors: Roberto
authors: R
authors: Matorras
authors: Begoña
authors: B
authors: Prieto
authors: Sandra
authors: S
authors: González
authors: Daniel
authors: D
authors: Nagore
authors: Laureano
authors: L
authors: Simón
authors: Felix
authors: F
authors: Elortza
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D001707:Biopsy, Needle; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D010064:Embryo Implantation; D016889:Endometrial Neoplasms; D004715:Endometriosis; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008183:Luteal Phase / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism; D010455:Peptides; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D000071165:Trefoil Factor-3
chemical_list: D009077:Mucins; D010455:Peptides; D020543:Proteome; C082495:TFF3 protein, human; D000071165:Trefoil Factor-3