title: Modulation of the conductance-voltage relationship of the BK(Ca) channel by shortening the cytosolic loop connecting two RCK domains.
abstract: Calcium-dependent gating of large-conductance calcium-activated potassium (BK(Ca)) channels is mediated by the intracellular carboxyl terminus, which contains two domains of regulator of K(+) conductance (RCK). In mammalian BK(Ca) channels, the two RCK domains are separated by a protein segment of 101 residues that is poorly conserved in evolution and predicted to have no regular secondary structures. We investigated the functional importance of this loop using a series of deletion mutations. We found that the length, rather than the specific sequence at the central region of the segment, is critical for the functionality of the channel. As the length of the loop is progressively shorted, the conductance-voltage relationship gradually shifts toward more positive voltages with a minimum length of 70 amino acids, in an apparent response to increased tension within the loop. Thus, the functional activity of the BK(Ca) channel can be modulated by altering the tension of this loop region.
authors: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors: Ju-Ho
authors: JH
authors: Lee
authors: Hyun-Ju
authors: HJ
authors: Kim
authors: Hae-Deun
authors: HD
authors: Kim
authors: Byoung-Cheol
authors: BC
authors: Lee
authors: Jang-Soo
authors: JS
authors: Chun
authors: Chul-Seung
authors: CS
authors: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019921:Biotinylation; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003720:Densitometry; D015151:Immunoblotting; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D008564:Membrane Potentials / Q000502:physiology; D009154:Mutation; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D051381:Rats; D017385:Sequence Homology
chemical_list: C494842:Kcnma1 protein, rat; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D015221:Potassium Channels