title: Development of reverse-transcription loop-mediated isothermal amplification for the detection of infectious bursal disease virus.
abstract: To establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of infectious bursal disease virus (IBDV), four primers specific to six regions of the VP3 gene were designed; the VP3 region was selected because it is a conserved part of the IBDV genome. After amplification in an isothermal water bath for 70 min, samples containing IBDV generated the expected ladder-like products while other viruses generated no product. The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation. The assay was significantly more sensitive than normal gel-based RT-PCR. Because it is specific and simple, the RT-LAMP assay can be widely applied in clinical laboratories for rapid detection of IBVD.
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
authors: Jiangtao
authors: J
authors: Xu
authors: Zhenmei
authors: Z
authors: Zhang
authors: Yanbo
authors: Y
authors: Yin
authors: Shangjin
authors: S
authors: Cui
authors: Shouzhen
authors: S
authors: Xu
authors: Yanyan
authors: Y
authors: Guo
authors: Jida
authors: J
authors: Li
authors: Jianlin
authors: J
authors: Wang
authors: Xingcai
authors: X
authors: Liu
authors: Limin
authors: L
authors: Han
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D002642:Chick Embryo; D002645:Chickens / Q000821:virology; D017931:DNA Primers; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; C478244:VP3 protein, infectious bursal disease virus; D015678:Viral Structural Proteins