Gulbenkiania indica sp. nov., isolated from a sulfur spring.
A novel bacterium, designated strain HT27(T), was isolated from a sulfur spring sample collected from Athamallik, Orissa, India, and was characterized by using a polyphasic approach. Cells were Gram-negative, strictly aerobic, rod-shaped and motile by means of a single polar flagellum. Strain HT27(T) was oxidase- and catalase-positive. Growth was observed at pH 5.0-11.0 and at 15-45 degrees C; the highest growth yield was observed at pH 7.5-8.0 and 30-37 degrees C. The G+C content of the genomic DNA of strain HT27(T) was 63 mol%. The major cellular fatty acids were C(16 : 1)omega7c (44.24 %), C(16 : 0) (27.65 %), C(18 : 1)omega7c (13.98 %), C(12 : 0) (2.60 %) and C(12 : 0) 3-OH (2.22 %). 16S rRNA gene sequence analysis indicated that strain HT27(T) clustered with the genus Gulbenkiania and showed 99.0 % similarity to Gulbenkiania mobilis E4FC31(T). However, the level of DNA-DNA relatedness between strain HT27(T) and G. mobilis E4FC31(T) was 30 %. On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA-DNA hybridization data, strain HT27(T) is considered to represent a novel species of the genus Gulbenkiania, for which the name Gulbenkiania indica sp. nov. is proposed. The type strain is HT27(T) (=DSM 17901(T) =JCM 15969(T)).
Jyoti
V
V
Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar 751 023, India.
Narayan
Kunwar Digvijay
KD
Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar 751 023, India.
Das
Subrata K
SK
Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar 751 023, India.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D045482:Hot Springs / Q000737:chemistry / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013455:Sulfur
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013455:Sulfur