A novel sandwich enzyme-linked immunosorbent assay for feline insulin.
A novel sandwich enzyme-linked immunosorbent assay (ELISA) was established to determine the serum insulin concentrations in domestic cats. By using a solid-phase mouse anti-bovine insulin monoclonal antibody and a peroxidase-conjugated guinea pig anti-rat insulin polyclonal antibody, feline serum insulin concentrations in the range of 0.1 to 3.6 ng/ml could be measured. The intraassay CV and interassay CV were less than 6% and less than 10%, respectively. The present insulin assay will strongly help studies on feline diabetes mellitus.
Nakaya
Mariko
M
Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Kito
Yuko
Y
Matsuki
Naoaki
N
Shibata
Haruki
H
Touhata
Yuki
Y
Tamahara
Satoshi
S
Ono
Kenichiro
K
D000818:Animals; D001786:Blood Glucose; D002415:Cats / Q000097:blood*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D007328:Insulin / Q000097:blood*; D008297:Male; D051379:Mice; D051381:Rats; D012680:Sensitivity and Specificity
D001786:Blood Glucose; D007328:Insulin