Antioxidant activities of flavonol derivatives from the leaves and stem bark of Scutia buxifolia Reiss.
This study evaluated the antioxidant activities in the leaves and stem bark fractions of Scutia buxifolia. Cerebral lipid peroxidation (TBARS) was induced by Fe(II) and radical-scavenging activity was determined by DPPH method. Folin-Ciocalteu was used to determine phenolic contents. Quercetin, quercitrin, isoquercitrin and rutin were isolated from leaf ethyl acetate fraction and their levels were measured by high performance liquid chromatography-photodiode array detector. IC(50) (DPPH) varied from 4.35+/-1.30 to 29.55+/-0.54 microg/mL for stem bark and from 6.50+/-0.40 to 30.54+/-1.14 in the leaves. Ethyl acetate and butanolic fractions caused a sharp fall in TBARS production with IC(50) from 2.93+/-2.17 to 40.46+/-2.51 microg/mL for the leaves and 0.66+/-0.17 to 27.3+/-1.23 for the stem bark. Results obtained indicated that S. buxifolia has a great potential to prevent disease caused by the overproduction of free radicals and also it might be used as a potential source of natural antioxidant agents.
Boligon
Aline Augusti
AA
Departamento de Farmácia Industrial, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil.
Pereira
Romaiana Picada
RP
Feltrin
Andriéli Cassel
AC
Machado
Michel Mansur
MM
Janovik
Vanessa
V
Rocha
João Batista Teixeira
JB
Athayde
Margareth Linde
ML
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects; D010636:Phenols / Q000378:metabolism; D010851:Picrates / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D051381:Rats; D027823:Rhamnaceae / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001713:Biphenyl Compounds; D044948:Flavonols; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron