A diet enriched in polyphenols and polyunsaturated fatty acids, LMN diet, induces neurogenesis in the subventricular zone and hippocampus of adult mouse brain.
At present it is widely accepted that there are at least two neurogenic sites in the adult mammalian brain: the subventricular zone (SVZ) of lateral ventricles and the subgranular zone (SGZ) of the hippocampus dentate gyrus. The adult proliferation rate declines with aging and is altered in several neurodegenerative pathologies including Alzheimer's disease. The aim of this work was to study whether a natural diet rich in polyphenols and polyunsaturated fatty acids (LMN diet) can modulate neurogenesis in adult mice and give insight into putative mechanisms. Results with BrdU and PCNA demonstrated that the LMN fed mice had more newly generated cells in the SVZ and SGZ, and those with DCX (undifferentiated neurons) and tyrosine hydroxylase, calretinin, and calbindin (differentiated neurons) immunostainings and western blots demonstrated a significant effect on neuronal populations, strongly supporting a positive role of the LMN diet on adult neurogenesis. In primary rat neuron cultures, the LMN cream dramatically protected against damage caused by both hydrogen peroxide and Abeta(1-42), demonstrating a potent antioxidant effect that could play a major role in the normal adult neurogenesis and, moreover, the LMN diet could have a significant effect combating the cognitive function decline during both aging and neurodegenerative diseases such as Alzheimer's disease.
Valente
Tony
T
Departament de Bioquimica i Biologia Molecular, Institut de Neurociències, Facultat de Medicina, Torre M2, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. tonyvalente@gmail.com.
Hidalgo
Juan
J
Bolea
Irene
I
Ramirez
Bartolomé
B
Anglés
Neus
N
Reguant
Jordi
J
Morelló
José Ramón
JR
Gutiérrez
Cristina
C
Boada
Mercè
M
Unzeta
Mercedes
M
D016229:Amyloid beta-Peptides / Q000187:drug effects / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D002552:Cerebral Ventricles / Q000166:cytology* / Q000187:drug effects; D018891:Dentate Gyrus / Q000166:cytology* / Q000187:drug effects; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D004043:Dietary Fiber / Q000494:pharmacology*; D000089662:Doublecortin Protein; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D005527:Food, Fortified; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D019636:Neurodegenerative Diseases / Q000178:diet therapy / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments / Q000187:drug effects / Q000502:physiology; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols
D016229:Amyloid beta-Peptides; C000714847:Dcx protein, mouse; C000714848:Dcx protein, rat; D004042:Dietary Fats, Unsaturated; D004043:Dietary Fiber; D000089662:Doublecortin Protein; D005231:Fatty Acids, Unsaturated; D005419:Flavonoids; D010446:Peptide Fragments; D010636:Phenols; D059808:Polyphenols; C075222:amyloid beta-protein (1-42)