title: Identification of splenic reservoir monocytes and their deployment to inflammatory sites.
abstract: A current paradigm states that monocytes circulate freely and patrol blood vessels but differentiate irreversibly into dendritic cells (DCs) or macrophages upon tissue entry. Here we show that bona fide undifferentiated monocytes reside in the spleen and outnumber their equivalents in circulation. The reservoir monocytes assemble in clusters in the cords of the subcapsular red pulp and are distinct from macrophages and DCs. In response to ischemic myocardial injury, splenic monocytes increase their motility, exit the spleen en masse, accumulate in injured tissue, and participate in wound healing. These observations uncover a role for the spleen as a site for storage and rapid deployment of monocytes and identify splenic monocytes as a resource that the body exploits to regulate inflammation.
authors: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. fswirski@mgh.harvard.edu
authors: Filip K
authors: FK
authors: Swirski
authors: Matthias
authors: M
authors: Nahrendorf
authors: Martin
authors: M
authors: Etzrodt
authors: Moritz
authors: M
authors: Wildgruber
authors: Virna
authors: V
authors: Cortez-Retamozo
authors: Peter
authors: P
authors: Panizzi
authors: Jose-Luiz
authors: JL
authors: Figueiredo
authors: Rainer H
authors: RH
authors: Kohler
authors: Aleksey
authors: A
authors: Chudnovskiy
authors: Peter
authors: P
authors: Waterman
authors: Elena
authors: E
authors: Aikawa
authors: Thorsten R
authors: TR
authors: Mempel
authors: Peter
authors: P
authors: Libby
authors: Ralph
authors: R
authors: Weissleder
authors: Mikael J
authors: MJ
authors: Pittet
mesh_terms: D000804:Angiotensin II / Q000097:blood / Q000494:pharmacology; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D001854:Bone Marrow Cells / Q000502:physiology; D002454:Cell Differentiation; D002465:Cell Movement; D048429:Cell Size; D005260:Female; D007249:Inflammation / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D009203:Myocardial Infarction / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology*; D009206:Myocardium / Q000276:immunology* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D011945:Receptors, Angiotensin / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013156:Splenectomy
chemical_list: D000950:Antigens, Ly; C494192:Ly-6C antigen, mouse; D011945:Receptors, Angiotensin; D000804:Angiotensin II