title: Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
abstract: Recent advances in the genetic manipulation of mice have enabled us to generate transgenic and knockout mice. However, it is not easy to maintain these genetically-modified mice with the high-quality necessary to meet both scientific and legal requirements. RIKEN BRC has collected various transgenic, knockout, and conditional knockout mice. To examine the genetic modifications in these strains quickly and thoroughly, we established a simultaneous PCR test to detect multiple transgenes. We have called this, the "KO-survey". The PCR condition was optimized to detect neo, puro, pgk-neo, lacZ, and HSVtk-neo in set I, and hyg, IRES, cre, flp, and Gfp in set II. This "KO-survey" is useful for providing users with mouse strains of the highest genetic quality and accurate information on their genetic modifications.
authors: Experimental Animal Division, RIKEN BioResource Center, Koyadai,Tsukuba, Ibaraki, Japan.
authors: Hatsumi
authors: H
authors: Nakata
authors: Tomomi
authors: T
authors: Hashimoto
authors: Yukiko
authors: Y
authors: Seki
authors: Kazuyuki
authors: K
authors: Mekada
authors: Yuichi
authors: Y
authors: Obata
authors: Atsushi
authors: A
authors: Yoshiki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005838:Genotype; D006078:Government Programs; D007564:Japan; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D008822:Mice, Transgenic / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019076:Transgenes