[Studies on content determination of polysaccharides in Periploca forrestii].
OBJECTIVE
To establish a method for content determination of polysaccharides in Periplocaforrestii.


METHODS
The conversion coefficient of P. forrestii. polysaccharide to glucose was obtained by refined polysaccharides, and then the content of crude polysaccharides was determined by sulfuric-phenol method.


RESULTS
The content of crude polysaccharides in P. forrestii. was 54.68% (RSD 3.0%, n = 6), and the average recovery was 101.7% (RSD 6.0%, n =6).


CONCLUSIONS
The method was simple, rapid, and accurate.
Sun
Lei
L
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Qiao
Shanyi
S
Zhao
Yimin
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D031109:Periploca / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D004365:Drugs, Chinese Herbal; D010636:Phenols; D011134:Polysaccharides