[Expression and localization of macrophages and BMP2 in mucosa of tympanosclerosis].
OBJECTIVE
To detect the expression and localization of macrophages and BMP2 (bone morphogenetic protein 2) in the middle ear mucosa of TS, to explore its role in the pathogenesis of TS.


METHODS
Seventeen patients with TS were studied and 17 matching cases with simple chronic suppurative otitis media (COM) were enrolled as control. Immunohistochemistry was performed to detect the expression and localization of CD68 (the symbol of macrophage) and BMP2 in the middle ear mucosa. And the expression difference in the TS and COM were analyzed.


RESULTS
Positive cells of CD68 was mainly localized in the submucosa layer. Each group had 12 cases with positive expression of CD68 (70.59%) and had no difference in the number of positive cells (TS: 6.94 +/- 6.08; COM: 7.59 +/- 7.84; P=0.79). All the specimens had positive expression of BMP2 and BMP2 protein was expressed in the mucosa layer and submucosa layer. There was statistical difference in the number of BMP2 expression between TS and COM group. In TS group, all the sclerotic plaques were surrounded with macrophages and BMP2 positive cells. The cells surrounding sclerotic plaques were synchronously expressing CD68 positive and BMP2 positive.


CONCLUSIONS
Macrophages should take part in the pathogenesis of TS. BMP2 secreted by macrophage was important in formation of TS.
Liu
Xiang
X
Department of Otorhinolaryngology, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510120, China.
Li
Yuan
Y
Zheng
Yiqing
Y
Zhang
Zhigang
Z
D000293:Adolescent; D000328:Adult; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D004427:Ear Diseases / Q000378:metabolism* / Q000473:pathology; D004432:Ear, Middle / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012598:Sclerosis / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2