title: Does the clock make the poison? Circadian variation in response to pesticides.
abstract: BACKGROUND
  Circadian clocks govern daily physiological and molecular rhythms, and putative rhythms in expression of xenobiotic metabolizing (XM) genes have been described in both insects and mammals. Such rhythms could have important consequences for outcomes of chemical exposures at different times of day. To determine whether reported XM gene expression rhythms result in functional rhythms, we examined daily profiles of enzyme activity and dose responses to the pesticides propoxur, deltamethrin, fipronil, and malathion.
  
  
  RESULTS
  Published microarray expression data were examined for temporal patterns. Male Drosophila were collected for ethoxycoumarin-O-deethylase (ECOD), esterase, glutathione-S-transferase (GST), and, and uridine 5'-diphosphoglucosyltransferase (UGT) enzyme activity assays, or subjected to dose-response tests at four hour intervals throughout the day in both light/dark and constant light conditions. Peak expression of several XM genes cluster in late afternoon. Significant diurnal variation was observed in ECOD and UGT enzyme activity, however, no significant daily variation was observed in esterase or GST activity. Daily profiles of susceptibility to lethality after acute exposure to propoxur and fipronil showed significantly increased resistance in midday, while susceptibility to deltamethrin and malathion varied little. In constant light, which interferes with clock function, the daily variation in susceptibility to propoxur and in ECOD and UGT enzyme activity was depressed.
  
  
  CONCLUSIONS
  Expression and activities of specific XM enzymes fluctuate during the day, and for specific insecticides, the concentration resulting in 50% mortality varies significantly during the day. Time of day of chemical exposure should be an important consideration in experimental design, use of pesticides, and human risk assessment.
authors: Department of Zoology, Oregon State University, Corvallis, Oregon, United States of America. hoovenl@onid.orst.edu
authors: Louisa A
authors: LA
authors: Hooven
authors: Katherine A
authors: KA
authors: Sherman
authors: Shawn
authors: S
authors: Butcher
authors: Jadwiga M
authors: JM
authors: Giebultowicz
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D004330:Drosophila / Q000201:enzymology / Q000502:physiology*; D008027:Light; D008297:Male; D010575:Pesticides / Q000494:pharmacology*
chemical_list: D010575:Pesticides