title: Hypermetabolism, hyperphagia, and reduced adiposity in tankyrase-deficient mice.
abstract: OBJECTIVE
  Tankyrase (TNKS) is a Golgi-associated poly-ADP-ribose polymerase that is implicated in the regulation of GLUT4 trafficking in 3T3-L1 adipocytes. Its chromosomal locus 8p23.1 is linked to monogenic forms of diabetes in certain kindred. We hypothesize that TNKS is involved in energy homeostasis in mammals.
  
  
  METHODS
  Gene-trap techniques were used to ablate TNKS expression in mice. Homozygous and wild-type littermates maintained on standard chow were compared.
  
  
  RESULTS
  Wild-type mice express the TNKS protein abundantly in adipose tissue, the brain, and the endocrine pancreas but scarcely in the exocrine pancreas and skeletal muscle. TNKS-deficient mice consume increased amounts of food (by 34%) but have decreased plasma leptin levels and a >50% reduction in epididymal and perirenal fat pad size. Their energy expenditure is increased as assessed by metabolic cage studies and core body temperatures. These changes are not attributable to an increase in physical activity or uncoupled respiration (based on oxygraph analyses of mitochondria isolated from brown fat and skeletal muscle). The heightened thermogenesis of TNKS-deficient mice is apparently fueled by increases in both fatty acid oxidation (based on muscle and liver gene expression analyses and plasma ketone levels) and insulin-stimulated glucose utilization (determined by hyperinsulinemic-euglycemic clamps). Although TNKS deficiency does not compromise insulin-stimulated GLUT4 translocation in primary adipocytes, it leads to the post-transcriptional upregulation of GLUT4 and adiponectin in adipocytes and increases plasma adiponectin levels.
  
  
  CONCLUSIONS
  TNKS-deficient mice exhibit increases in energy expenditure, fatty acid oxidation, and insulin-stimulated glucose utilization. Despite excessive food intake, their adiposity is substantially decreased.
authors: Department of Medicine, Endocrine Division, University of California, San Diego, La Jolla, California, USA.
authors: Tsung-Yin J
authors: TY
authors: Yeh
authors: Kristina K
authors: KK
authors: Beiswenger
authors: Pingping
authors: P
authors: Li
authors: Krista E
authors: KE
authors: Bolin
authors: Ray M
authors: RM
authors: Lee
authors: Tsu-Shuen
authors: TS
authors: Tsao
authors: Anne N
authors: AN
authors: Murphy
authors: Andrea L
authors: AL
authors: Hevener
authors: Nai-Wen
authors: NW
authors: Chi
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D002001:Adipose Tissue, Brown / Q000378:metabolism; D000818:Animals; D001755:Blastocyst; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D053595:Embryonic Stem Cells / Q000378:metabolism; D004734:Energy Metabolism / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006019:Glycolysis / Q000187:drug effects; D006579:Heterozygote; D006801:Humans; D006963:Hyperphagia / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D020738:Leptin / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008931:Mitochondria, Muscle / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D010101:Oxygen Consumption; D038501:Tankyrases / Q000172:deficiency* / Q000235:genetics
chemical_list: D005227:Fatty Acids; D007328:Insulin; D020738:Leptin; D038501:Tankyrases