Efficient incorporation of free oxygen into volicitin in Spodoptera litura common cutworm larvae.
Volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] has previously been identified from the lepidopteran larval regurgitant as an elicitor of plant volatile emission. The efficient incorporation of free oxygen into volicitin by Spodoptera litura larvae is demonstrated here by rearing them under (18)O(2) for three days. (18)O-labeling of the hydroxyl group of volicitin was confirmed by liquid chromatography/mass spectrometry-ion trap-time-of-flight (LC/MS-IT-TOF) and suggests the activity of a monooxygenase in volicitin biosynthesis.
Ishikawa
Chihiro
C
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
Yoshinaga
Naoko
N
Aboshi
Takako
T
Nishida
Ritsuo
R
Mori
Naoki
N
D000818:Animals; D005973:Glutamine / Q000031:analogs & derivatives* / Q000378:metabolism; D006900:Hydroxylation; D007553:Isotope Labeling; D007814:Larva / Q000378:metabolism; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D018411:Spodoptera / Q000378:metabolism*; D017962:alpha-Linolenic Acid / Q000031:analogs & derivatives* / Q000378:metabolism
C419781:volicitin; D017962:alpha-Linolenic Acid; D005973:Glutamine; D010100:Oxygen