Niastella populi sp. nov., isolated from soil of Euphrates poplar (Populus euphratica) forest, and emended description of the genus Niastella.
A novel bacterial strain, designated THYL-44(T), was isolated from the soil of a Euphrates poplar (Populus euphratica) forest in Xinjiang, China. The cells were strictly aerobic, Gram-staining-negative, non-flagellated, non-motile and filamentous. Growth occurred at 17-37 degrees C (optimum 30 degrees C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-1 % NaCl (w/v; optimum 0 %). Flexirubin pigments were not produced. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain THYL-44(T) was closely related to Niastella koreensis KACC 11465(T) (95.5 % sequence similarity). The major respiratory quinone was MK-7 and the predominant cellular fatty acids were iso-C(15 : 0) (28.6 %), iso-C(17 : 0) 3-OH (23.9 %) and iso-C(15 : 1) G (17.4 %). The DNA G+C content was 45.2 mol%. Therefore, the phylogenetic, physiological and chemotaxonomic data demonstrated that strain THYL-44(T) represents a novel species of the genus Niastella , for which the name Niastella populi sp. nov. is proposed. The type strain is THYL-44(T) (=CCTCC AB 208238(T)=KCTC 22560(T)). On the basis of new data, an emended description of the genus Niastella is also proposed.
Zhang
Kundi
K
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Wang
Yang
Y
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Tang
Yali
Y
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Dai
Jun
J
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Zhang
Lei
L
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
An
Hongli
H
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Luo
Guangchao
G
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Rahman
Erkin
E
College of Life Science and Technology, Xinjiang University, Urumchi 830046, PR China.
Fang
Chengxiang
C
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S