Saccharopolyspora gloriosae sp. nov., an endophytic actinomycete isolated from the stem of Gloriosa superba L.
A Gram-stain-positive, aerobic actinomycete, strain YIM 60513(T), was isolated from the stem of Gloriosa superba L. collected from tropical rainforest at Xishuangbanna, Yunnan Province, south-west China. 16S rRNA gene sequence analysis indicated that strain YIM 60513(T) belonged to the genus Saccharopolyspora and was closely related to Saccharopolyspora gregorii NCIB 12823(T) (99.1 % similarity) and Saccharopolyspora cebuensis SPE 10-1(T) (97.3 % similarity). Data for the predominant quinone [MK-9(H(4))], major fatty acids (iso-C(16 : 0), anteiso-C(17 : 0) and C(17 : 1) cis9) and G+C content of the genomic DNA (71.6 mol%) were similar to those for members of the genus Saccharopolyspora. The level of DNA-DNA relatedness between strain YIM 60513(T) and S. gregorii NCIB 12823(T) was 43 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic characteristics and DNA-DNA hybridization data supported the view that strain YIM 60513(T) should be distinguished from S. gregorii NCIB 12823(T) and S. cebuensis SPE 10-1(T). Strain YIM 60513(T) therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora gloriosae sp. nov. is proposed. The type strain is YIM 60513(T) (=KCTC 19243(T) =CCTCC AA 207006(T)).
Qin
Sheng
S
The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
Chen
Hua-Hong
HH
Department of Chemistry and Biology Science, Chuxiong Normal University, Chuxiong, Yunnan, 675000, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
Klenk
Hans-Peter
HP
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, D-38124 Braunschweig, Germany.
Kim
Chang-Jin
CJ
Functional Metabolite Research Center, KRIBB, 52 Eoeun-dong, Yuseong gu, Daejeon 305-333, Republic of Korea.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D019668:Liliaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018547:Plant Stems / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016930:Saccharopolyspora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014197:Trees; D014329:Tropical Climate
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S