Mapping protein abundance patterns in the brain using voxelation combined with liquid chromatography and mass spectrometry.
Voxelation creates expression atlases by high-throughput analysis of spatially registered cubes or voxels harvested from the brain. The modality independence of voxelation allows a variety of bioanalytical techniques to be used to map abundance. Protein expression patterns in the brain can be obtained using liquid chromatography (LC) combined with mass spectrometry (MS). Here we describe the methodology of voxelation as it pertains particularly to LC-MS proteomic analysis: sample preparation, instrumental set up and analysis, peptide identification and protein relative abundance quantitation. We also briefly describe some of the advantages, limitations and insights into the brain that can be obtained using combined proteomic and transcriptomic maps.
Petyuk
Vladislav A
VA
Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Qian
Wei-Jun
WJ
Smith
Richard D
RD
Smith
Desmond J
DJ
D000465:Algorithms; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D010103:Oxygen Isotopes / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods; D014158:Transcription, Genetic
D010103:Oxygen Isotopes; D010455:Peptides; D020543:Proteome