title: Enhancement of human gamma-interferon production in recombinant E. coli using batch cultivation.
abstract: Development of inexpensive and simple culture media and appropriate induction conditions are always favorable for industry. In this research, chemical composition and stoichiometric data for gamma-interferon production and recombinant Escherichia coli growth were used in order to achieve a simple medium and favorable induction conditions. To achieve this goal, the effects of medium composition and induction conditions on the production of gamma-interferon were investigated in batch culture of E. coli BL21 (DE3) [pET3a-ifngamma]. These conditions were considered as suitable conditions for the production of gamma-interferon: 2.5x M9 medium, supplemented with a mixture of amino acids (milligram per liter), including glutamic acid 215, aspartic acid 250, lysine 160, and phenylalanine 90, and induction at late-log phase (OD(600) = 4.5). Under these conditions, dry cell weight of 6 +/- 0.2 g/l and gamma-interferon concentration of 2.15 +/- 0.1 g/l were obtained. Later, without changing the concentration ratio of amino acids and glucose, the effect of increase in the primary glucose concentration on productivity of gamma-interferon was investigated. It was found that 25 g/l glucose will result in maximum attainable biomass and recombinant human gamma-interferon. At improved conditions, a dry cell weight of 14 +/- 0.2 g/l, concentration and overall productivity of gamma-interferon 4.2 +/- 0.1 g/l and 420 +/- 10 mg/l h, respectively, were obtained.
authors: Group of Biotechnology, Department of Chemical Engineering, Faculty of Engineering, University of Tarbiat Modares, PO Box 14115-143, Tehran, Iran.
authors: Valiollah
authors: V
authors: Babaeipour
authors: Seyed Abbas
authors: SA
authors: Shojaosadati
authors: Rasoul
authors: R
authors: Khalilzadeh
authors: Nader
authors: N
authors: Maghsoudi
authors: Amir Mohammad
authors: AM
authors: Farnoud
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D007371:Interferon-gamma; D005947:Glucose