Thalassospira xianhensis sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium.
A polycyclic aromatic hydrocarbon-degrading marine bacterium, designated strain P-4(T), was isolated from oil-polluted saline soil in Xianhe, Shangdong Province, China. Strain P-4(T) was Gram-negative-staining with curved to spiral rod-shaped cells and grew optimally with 3-6 % (w/v) NaCl and at 30 degrees C. The predominant fatty acids were C(18 : 1)omega7c (35.0 %), C(16 : 0) (25.0 %), C(16 : 1)omega7c (17.9 %), C(14 : 0) (6.2 %) and C(17 : 0) cyclo (5.2 %). The major respiratory quinone was Q-9 and the genomic DNA G+C content was 61.2+/-1.0 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain P-4(T) belonged to the genus Thalassospira of the class Alphaproteobacteria. DNA-DNA hybridization with Thalassospira xiamenensis DSM 17429(T) showed relatedness of 36.0 %, and lower values were obtained with respect to other Thalassospira species. Based on physiological and biochemical tests and 16S rRNA gene sequence analysis as well as DNA-DNA relatedness, strain P-4(T) should be placed in the genus Thalassospira within a novel species. The name Thalassospira xianhensis sp. nov. is proposed, with P-4(T) (=CGMCC 1.6849(T) =JCM 14850(T)) as the type strain.
Zhao
Baisuo
B
Department of Environmental Science and Engineering, State Key Joint Laboratory on Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, PR China.
Wang
Hui
H
Department of Environmental Science and Engineering, State Key Joint Laboratory on Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, PR China.
Li
Ruirui
R
Department of Environmental Science and Engineering, State Key Joint Laboratory on Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, PR China.
Mao
Xinwei
X
Department of Environmental Science and Engineering, State Key Joint Laboratory on Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, PR China.
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum; D010641:Phenotype; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012989:Soil Pollutants; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D012965:Sodium Chloride