Antitumor activity and DNA-binding investigations of the Zn(II) and Cu(II) complexes with isoeuxanthone.
Two new complexes ZnL(2) (1) and CuL(2) (2) (here, HL=isoeuxanthone) have been synthesized and characterized by elemental analyses, molar conductance, infrared spectra (IR), (1)H-NMR and UV-Vis measurements. The interactions of them with calf thymus DNA (ct DNA) have been investigated by absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy and viscosity measurements. Experimental results revealed an intercalative interaction with DNA for the complexes; furthermore the binding affinity of 2 is higher than that of 1 according to the calculated binding constant values. In addition, they were evaluated for their cytotoxic activities toward human esophageal cancer (ECA109) and human gastric cancer (SGC7901) cells by MTT assay. Both of them showed significant cytotoxic potency.
Wang
Huifang
H
College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, P.R. China.
Shen
Rui
R
Wu
Jincai
J
Tang
Ning
N
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004563:Electrochemistry; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014783:Viscosity; D044004:Xanthones / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
D000970:Antineoplastic Agents; D008024:Ligands; D009942:Organometallic Compounds; D044004:Xanthones; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D015032:Zinc