Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord.
We isolated mesenchymal stem cells (MSC) from arteries (UCA), veins (UCV), and Wharton's jelly (UCWJ) of human umbilical cords (UC) and determined their relative capacities for sustained proliferation and multilineage differentiation. Individual UC components were dissected, diced into 1-2 mm(3) fragments, and aligned in explant cultures from which migrating cells were isolated using trypsinization. Preparations from 13 UCs produced 13 UCWJ, 11 UCV, and 10 UCA cultures of fibroblast-like, spindle-shaped cells negative for CD31, CD34, CD45, CD271, and HLA-class II, but positive for CD13, CD29, CD44, CD73, CD90, CD105, and HLA-class I. UCV cells exhibited a significantly higher frequency of colony-forming units fibroblasts than did UCWJ and UCA cells. Individual MSCs could be selectively differentiated into osteoblasts, chondrocytes, and adipocytes. When compared for osteogenic potential, UCWJ cells were the least effective precursors, whereas UCA-derived cells developed alkaline phosphatase activity with or without an osteogenic stimulus. UC components, especially blood vessels, could provide a promising source of MSCs with important clinical applications.
Ishige
Ikuo
I
Department of Cell Processing and Transfusion, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.|Division of Stem Cell Therapy, Center for Stem Cell and Regenerative Medicine, The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.
Nagamura-Inoue
Tokiko
T
Department of Cell Processing and Transfusion, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan. tokikoni@ims.u-tokyo.ac.jp.
Honda
Masaki J
MJ
Department of Anatomy, School of Dentistry, Nihon University, Kanda-Surugadai 1-8-13, Chiyoda-ku, Tokyo, 101-8310, Japan.
Harnprasopwat
Ratanakanit
R
Division of Molecular of Therapy, Center for Advanced Medical Research, The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.
Kido
Michiko
M
Department of Obstetrics, Japanese Red Cross Medical Center, Hiroo 4-1-22, Shibuya-ku, Tokyo, 150-8935, Japan.
Sugimoto
Mitsuhiro
M
Department of Obstetrics, Japanese Red Cross Medical Center, Hiroo 4-1-22, Shibuya-ku, Tokyo, 150-8935, Japan.
Nakauchi
Hiromitsu
H
Division of Stem Cell Therapy, Center for Stem Cell and Regenerative Medicine, The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.
Tojo
Arinobu
A
Department of Cell Processing and Transfusion, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.|Division of Molecular of Therapy, Center for Advanced Medical Research, The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.
D017667:Adipocytes / Q000166:cytology; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014469:Umbilical Arteries; D014470:Umbilical Cord; D014471:Umbilical Veins
D015415:Biomarkers