Flow cytometry with gold nanoparticles and their clusters as scattering contrast agents: FDTD simulation of light-cell interaction.
The formulation of the finite-difference time-domain (FDTD) approach is presented in the framework of its potential applications to in-vivo flow cytometry based on light scattering. The consideration is focused on comparison of light scattering by a single biological cell alone in controlled refractive-index matching conditions and by cells labeled by gold nanoparticles. The optical schematics including phase contrast (OPCM) microscopy as a prospective modality for in-vivo flow cytometry is also analyzed. The validation of the FDTD approach for the simulation of flow cytometry may open up a new avenue in the development of advanced cytometric techniques based on scattering effects from nanoscale targets.
Tanev
Stoyan
S
Technology Innovation Management Program in the Department of Systems and Computer Engineering, Faculty of Engineering and Design, Carleton University, Ottawa, ON, Canada. tan@ib.sdu.dk
Sun
Wenbo
W
Pond
James
J
Tuchin
Valery V
VV
Zharov
Vladimir P
VP
D002477:Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D003287:Contrast Media / Q000737:chemistry*; D003593:Cytoplasm / Q000378:metabolism; D004058:Diffusion; D005110:Extracellular Space / Q000378:metabolism; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D008858:Microscopy, Phase-Contrast; D008954:Models, Biological; D012542:Scattering, Radiation; D013997:Time Factors
D003287:Contrast Media; D006046:Gold