HSD11B1 polymorphisms predicted bone mineral density and fracture risk in postmenopausal women without a clinically apparent hypercortisolemia.
BACKGROUND
Endogenous glucocorticoid (GC) may participate in bone physiology, even in subjects with no glucocorticoid excess. 11beta-hydroxysteroid dehydrogenase type 1 (HSD11B1) is a primary regulator catalyzing the reduction of inactive cortisone to active cortisol. To elucidate genetic relevance of HSD11B1 variants to vertebral fracture and osteoporosis, we investigated the potential involvement of six HSD11B1 SNPs in postmenopausal women.


METHODS
All exons, their boundaries and the promoter region (approximately 1.5 kb) were directly sequenced in 24 individuals. Six polymorphisms were selected and genotyped in all study participants (n=1329). BMD was measured using dual-energy X-ray absorptiometry.


RESULTS
HSD11B1 +16374C>T and +27447G>C were associated with reduced vertebral fracture risk (p=0.016 and 0.032, respectively). Two of these (LD block2) in intron 5 (rs1000283 and rs932335) were significantly associated with bone mineral density (BMD) at the femoral neck (p=0.00005 and 0.0002, respectively). Specifically, HSD11B1 +16374C>T and +27447G>C polymorphisms were associated with higher BMD values of the femoral neck in multiple comparison (p=0.0002 and 0.0004, respectively) and Bonferroni corrected significance level (97% power). Consistent with these results, HSD11B1-ht21 and -ht22 comprising both SNPs also showed the evidence of association with BMD values of the femoral neck (p(domiant)=0.0002 and p(recessive)=0.00005, respectively).


CONCLUSIONS
Our results provide preliminary evidence supporting an association of HSD11B1 with osteoporosis in postmenopausal women. Also, these findings demonstrate that +16374C>T polymorphism may be useful genetic markers for bone metabolism.
Hwang
Joo-Yeon
JY
The Center for Genome Science, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul, 122-701, Republic of Korea.
Lee
Seung Hun
SH
Kim
Ghi Su
GS
Koh
Jung-Min
JM
Go
Min Jin
MJ
Kim
Young-Jin
YJ
Kim
Hyung-Cheol
HC
Kim
Tae-Ho
TH
Hong
Jung Min
JM
Park
Eui Kyun
EK
Lee
Jong-Young
JY
Kim
Shin-Yoon
SY
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics*; D015519:Bone Density / Q000235:genetics / Q000502:physiology*; D003480:Cushing Syndrome / Q000150:complications* / Q000201:enzymology / Q000235:genetics; D005260:Female; D005272:Femur Neck / Q000503:physiopathology; D050723:Fractures, Bone / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017698:Postmenopause / Q000235:genetics*; D012044:Regression Analysis; D012307:Risk Factors; D016103:Spinal Fractures / Q000201:enzymology / Q000235:genetics
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1