Labelling of mammalian cells for visualisation by MRI.
Through labelling of cells with magnetic contrast agents it is possible to follow the fate of transplanted cells in vivo with magnetic resonance imaging (MRI) as has been demonstrated in animal studies as well as in a clinical setting. A large variety of labelling strategies are available that allow for prolonged and sensitive detection of the labelled cells with MRI. The various protocols each harbour specific advantages and disadvantages. In choosing a particular labelling strategy it is also important to ascertain that the labelling procedure does not negatively influence cell functionality, for which a large variety of assays are available. In order to overcome the challenges still faced in fully exploiting the benefits of in vivo cell tracking by MRI a good understanding and standardisation of the procedures and assays used will be crucial.
Bernsen
Monique R
MR
Department of Radiology, Erasmus MC-University Medical Center Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands. m.bernsen@erasmusmc.nl
Moelker
Amber D
AD
Wielopolski
Piotr A
PA
van Tiel
Sandra T
ST
Krestin
Gabriel P
GP
D000818:Animals; D002478:Cells, Cultured / Q000166:cytology*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods* / Q000639:trends*; D015336:Molecular Probe Techniques / Q000639:trends*; D013194:Staining and Labeling / Q000379:methods / Q000639:trends