title: Purification and characterization of a tuliposide-converting enzyme from bulbs of Tulipa gesneriana.
abstract: An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral pH and moderate temperature. The enzyme preferentially acted on such glucose esters as 6-tuliposides, and to a lesser extent on p-nitrophenylacetate.
authors: Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Japan. ykato@pu-toyama.ac.jp
authors: Yasuo
authors: Y
authors: Kato
authors: Kazuaki
authors: K
authors: Shoji
authors: Makoto
authors: M
authors: Ubukata
authors: Kengo
authors: K
authors: Shigetomi
authors: Yukio
authors: Y
authors: Sato
authors: Noriyuki
authors: N
authors: Nakajima
authors: Shinjiro
authors: S
authors: Ogita
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism; D055162:Biocatalysis; D006027:Glycosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D018520:Plant Shoots / Q000201:enzymology* / Q000378:metabolism; D012978:Sodium Oxybate / Q000031:analogs & derivatives* / Q000378:metabolism; D013696:Temperature; D031447:Tulipa / Q000201:enzymology* / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D006027:Glycosides; C046169:6-tuliposide A; D012978:Sodium Oxybate