The BB genotype of heat-shock protein (HSP) 70-2 gene is associated with gastric pre-malignant condition in H. pylori-infected older patients.
BACKGROUND
Heat-shock protein (HSP) 70 plays essential roles in cellular response to a variety of environmental stresses or unfavorable conditions. A to G transition at the 1267 position of the HSP70-2 gene confers different levels of HSP70 mRNA expression. We aimed to clarify the effect of HSP70-2 polymorphism on the risk of premalignant condition, on the degree of acute or chronic inflammation in the stomach.


METHODS
A total of 378 individuals participated in this study. Restriction fragment length polymorphism analysis was performed for polymorphisms at 1267 of HSP70-2 gene. Prevalence of intestinal metaplasia was investigated histologically and the degree of histological gastritis in the antrum was classified according to the updated Sydney System.


RESULTS
Although a direct association was not observed between HSP70-2 polymorphism and prevalence of intestinal metaplasia, a significant association was found between the BB genotype and lower metaplasia score in individuals who were Helicobacter pylori (H. pylori) positive and aged 60 years or older (BB vs. A carriers; 0.84+/-0.95 vs. 1.23+/-1.01, p=0.0197). When individuals were divided into 3 groups according to the severity of gastric mucosal atrophy: non-atrophic gastritis (NA) group (atrophy score=0 and metaplasia score=0), severe atrophic gastritis (SA) group (atrophy score > or =2 or metaplasia score > or =2), and mild atrophic gastritis (MA) group (all others), the BB genotype was associated with a lower risk of severe atrophy in the SA sub-group (adjusted odds ratio=0.37, 95% confidence interval =0.16-0.84, p=0.0172).


CONCLUSIONS
The BB genotype of HSP70-2 gene is associated with a reduced risk of gastric pre-malignant condition in H. pylori-infected older individuals.
Tahara
Tomomitsu
T
Department of Gastroenterology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan. tomomiccyu@yahoo.co.jp
Shibata
Tomoyuki
T
Arisawa
Tomiyasu
T
Nakamura
Masakatsu
M
Yoshioka
Daisuke
D
Okubo
Masaaki
M
Maruyama
Naoko
N
Kamano
Toshiaki
T
Kamiya
Yoshio
Y
Fujita
Hiroshi
H
Nagasaka
Mitsuo
M
Iwata
Masami
M
Yamashita
Hiromi
H
Nakano
Hiroshi
H
Hirata
Ichiro
I
D004273:DNA, Neoplasm / Q000235:genetics; D018900:Dinucleotide Repeats / Q000235:genetics; D005260:Female; D005757:Gastritis, Atrophic / Q000235:genetics* / Q000382:microbiology; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D016481:Helicobacter Infections / Q000235:genetics* / Q000382:microbiology; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D007410:Intestinal Diseases / Q000235:genetics* / Q000382:microbiology; D008297:Male; D008679:Metaplasia / Q000235:genetics* / Q000382:microbiology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics* / Q000382:microbiology; D012307:Risk Factors; D013270:Stomach / Q000382:microbiology / Q000473:pathology
D004273:DNA, Neoplasm; D018840:HSP70 Heat-Shock Proteins; C525621:HSPA1B protein, human