Comparison of cytokine expression in mesenchymal stem cells from human placenta, cord blood, and bone marrow.
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROalpha(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.
Hwang
Jong Ha
JH
Department of Obstetrics and Gynecology, School of Medicine, Korea University, Seoul, Korea.
Shim
Soung Shin
SS
Seok
Oye Sun
OS
Lee
Hang Young
HY
Woo
Sang Kyu
SK
Kim
Bong Hui
BH
Song
Hae Ryong
HR
Lee
Jae Kwan
JK
Park
Yong Kyun
YK
D001854:Bone Marrow Cells / Q000166:cytology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D040081:Protein Array Analysis
D016207:Cytokines