Paenibacillus hunanensis sp. nov., isolated from rice seeds.
Four Gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains FeL05(T), FeL11, Fek19 and Fek21, were isolated from seeds of hybrid rice (Oryza sativa L. Jinyou 611), and their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains were members of the genus Paenibacillus. They showed 95.4 % sequence similarity or less with strains of other Paenibacillus species. The G+C content of strain FeL05(T) was found to be 53.3 mol%. Its predominant respiratory quinone was MK-7. The predominant cellular fatty acids were anteiso-C(15 : 0) (61.7 %), C(16 : 0) (10.9 %), iso-C(16 : 0) (7.0 %), anteiso-C(17 : 0) (6.7 %) and iso-C(15 : 0) (5.2 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain FeL05(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hunanensis sp. nov. is proposed. The type strain is strain FeL05(T) (=ACCC 10718(T) =CGMCC 1.8907(T) =DSM 22170(T)).
Liu
Yang
Y
College of Life Sciences, Capital Normal University, Beijing 100048, PR China.
Liu
Lin
L
College of Life Sciences, Capital Normal University, Beijing 100048, PR China.
Qiu
Fubin
F
College of Public Health, Shanxi Medical University, Taiyuan 030001, PR China.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Shi
Yu
Y
Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Zou
Yuanyuan
Y
College of Life Sciences, Capital Normal University, Beijing 100048, PR China.
Zhang
Xiaoxia
X
Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Song
Wei
W
College of Life Sciences, Capital Normal University, Beijing 100048, PR China.
D001482:Base Composition; D016227:Benzoquinones / Q000378:metabolism; D002681:China; D017931:DNA Primers; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D056507:Paenibacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012639:Seeds / Q000382:microbiology*
D016227:Benzoquinones; D017931:DNA Primers; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C004532:quinone