title: Correlation between usage of macrolide antibiotic and resistance of Streptococcus pneumoniae clinic isolates from Chongqing children's hospital.
abstract: To investigate the reasons of growing resistance problem of Streptococcus pneumoniae against macrolide in Chongqing, a retrospective method was employed to measure the minimal inhibition concentrations (MIC) of macrolide antibiotic against 1,210 S. pneumoniae clinic isolates. The defined daily doses (DDDs) of macrolide antibiotic were calculated. Polymerase chain reaction (PCR) was used to determine the presence of the erythromycin-resistant genes in 100 macrolide-resistant S. pneumoniae isolates. A decrease in macrolide consumption, from 371,100 DDDs in 2002 to 182,500 DDDs in 2005 (51% reduction); however, the rate of erythromycin resistance in S. pneumoniae showed continued increase from 88.0% in 2002 to 96.0% in 2005. No linear correlation was observed between the decline in macrolide consumption and continued increase in resistant rate in S. pneumoniae. In 100 macrolide-resistant S. pneumoniae isolates, 68 had both erm(B) and mef(A) genotypes, 10 only had the erm(B), 20 only had the mef(A). Co-existences of ribosomal modification coded by erm(B) gene and efflux effects coded by mef(A) gene were the main resistance mechanism against macrolides and might be attributed to the high drug resistance of S. pneumoniae in Chongqing.
authors: Chongqing Children's Hospital, Chongqing Medical University, Chongqing, China.
authors: Jiong
authors: J
authors: Chen
authors: Lan
authors: L
authors: Liu
authors: Gang
authors: G
authors: Wang
authors: Yuan
authors: Y
authors: Chen
authors: Zhengxiu
authors: Z
authors: Luo
authors: Ying
authors: Y
authors: Huang
authors: Zhou
authors: Z
authors: Fu
authors: Yonghong
authors: Y
authors: Yang
authors: Enmei
authors: E
authors: Liu
mesh_terms: D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects* / Q000235:genetics; D006776:Hospitals, Pediatric; D006801:Humans; D018942:Macrolides / Q000627:therapeutic use*; D011008:Pneumococcal Infections / Q000188:drug therapy* / Q000453:epidemiology; D015995:Prevalence; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics
chemical_list: D018942:Macrolides