Translocation detection in lymphoma diagnosis by split-signal FISH: a standardised approach.
Lymphomas originating from the lymphatic system comprise about 30 entities classified according to the World Health Organization (WHO). The histopathological diagnosis is generally considered difficult and prone to mistakes. Since non-random chromosomal translocations are specifically involved in different lymphoma entities, their detection will be increasingly important. Hence, a split-signal fluorescence in situ hybridisation (FISH) procedure would be helpful in discriminating the most difficult classifications. The Euro-FISH programme, a concerted action of nine European laboratories, has validated a robust, standardised protocol to improve the diagnostic approach on lymphoma entities. Therefore, 16 fluorescent probes and 10 WHO entities, supplemented with reactive cases, were selected. The results of the Euro-FISH programme show that all probes were correctly cytogenetically located, that the standardised protocol is robust, resulting in reliable results in approximately 90% of cases, and that the procedure could be implemented in every laboratory, bringing the relatively easy interpretation of split-signal probes within the reach of many pathology laboratories.
van Rijk
Anke
A
Department of Pathology-824, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands, a.vanrijk@pathol.umcn.nl.
Mason
David
D
Jones
Margaret
M
Cabeçadas
José
J
Crespo
Mateus
M
Cigudosa
Juan Cruz
JC
Garcia
Juan Fernando
JF
Leoncini
Lorenzo
L
Cocco
Mario
M
Hansmann
Martin-Leo
ML
Mottok
Anja
A
Copie Bergman
Christiane
C
Baia
Maryse
M
Anagnostou
Dimitra
D
Pouliou
Evi
E
Hamilton Dutoit
Stephen
S
Hjøllund Christiansen
Mette
M
Svenstrup Poulsen
Tim
T
Hauge Matthiesen
Steen
S
van Dongen
Jacques
J
van Krieken
J Han
JH