De novo follicular regeneration of the skin by wingless int 3 and bone morphogenetic protein 2 genes introduced into dermal fibroblasts and fibroblast growth factor-2 protein.
In this study, we regenerated skin and its appendages by transplanting cultured normal dermal fibroblasts, into which morphogen genes had been introduced. We cultured normal dermal fibroblasts obtained from Fisher 344 rats on the surface of hydroxyapatite beads, and then adsorbed them onto the surface of a collagen sponge, which was transplanted into a full-thickness skin defect prepared on the backs of rats. Before transplantation, genes were introduced into the dermal fibroblasts via adenovirus vector (ad)-bone morphogenetic protein 2 and ad-wingless int 3 genes in addition to fibroblast growth factor-2 protein. By Week 4, the appearance of follicle germs or primitive hair germs was observed only in the ad-bone morphogenetic protein 2+ad-wingless int 3 combined with the fibroblast growth factor-2 protein group. By Week 16, in that same group, hair follicles having mature pilosebaceous systems with equally spaced localization had formed in the ulcer wound.
Ono
Ichiro
I
Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan. ichiro@sapmed.ac.jp
Akasaka
Yoshikiyo
Y
Kamiya
Takafumi
T
Sato
Makito
M
Kobune
Masayoshi
M
Hamada
Hirofumi
H
Yamashita
Toshiharu
T
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D020405:Dermis / Q000166:cytology; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D005822:Genetic Vectors; D018859:Hair Follicle / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D012038:Regeneration / Q000502:physiology*; D016038:Skin Transplantation / Q000379:methods; D023822:Tissue Engineering; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism; D060508:Wnt3 Protein; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D029867:Xenopus Proteins
D055396:Bone Morphogenetic Protein 2; D051153:Wnt Proteins; D060508:Wnt3 Protein; D029867:Xenopus Proteins; D016222:Fibroblast Growth Factor 2