Mesorhizobium alhagi sp. nov., isolated from wild Alhagi sparsifolia in north-western China.
Eleven strains that formed symbiotic root nodules on Alhagi sparsifolia, designated previously as genospecies II, were identified as a new lineage of Mesorhizobium (Alphaproteobacteria) that could be differentiated from all previously recognized species of the genus Mesorhizobium by using 16S rRNA gene sequences (<97.8 % similarity), DNA-DNA hybridization (<45 %), dnaJ, dnaK, recA, glnA, nifH, nodA and nodC gene sequences, fatty acid profiles (C(18 : 1)omega7c, 35 %;11-methyl C(18 : 1)omega7c, 30 %) and numerical taxonomy. These strains are therefore considered to represent a novel species, for which the name Mesorhizobium alhagi sp. nov. is proposed, with isolate CCNWXJ12-2(T) (=ACCC 15461(T)=HAMBI 3019(T)) as the type strain.
Chen
Wei-Min
WM
Department of Biology, University of York, PO Box 373, York YO10 5YW, UK.|College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
Zhu
Wen-Fei
WF
College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
Bontemps
Cyril
C
Department of Biology, University of York, PO Box 373, York YO10 5YW, UK.
Young
J Peter W
JPW
Department of Biology, University of York, PO Box 373, York YO10 5YW, UK.
Wei
Ge Hong
GH
College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013559:Symbiosis
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S