Promicromonospora umidemergens sp. nov., isolated from moisture from indoor wall material.
A Gram-positive, yellow-pigmented, branched-hyphae-forming micro-organism, strain 09-Be-007(T), was isolated from the wall of an indoor environment. Based on 16S rRNA gene sequence similarity studies, strain 09-Be-007(T) belonged to the genus Promicromonospora. The novel isolate showed sequence similarities of 98.8 % to Promicromonospora aerolata V54A(T), 98.9 % to Promicromonospora vindobonensis V45(T), 98.1 % to Promicromonospora sukumoe DSM 44121(T), 98.2 % to Promicromonospora kroppenstedtii RS16(T), 96.7 % to Promicromonospora flava CC 0387(T) and 97.8 % to Promicromonospora citrea DSM 43110(T), the type strain of the type species of this genus. Cell wall sugars of strain 09-Be-007(T) were galactose, rhamnose and glucose. The diagnostic diamino acid of the cell-wall peptidoglycan was lysine. The major menaquinones detected were MK-9(H(4)) and MK-9(H(6)). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. Major fatty acids were iso-C(15 : 0) and anteiso-C(15 : 0); moderate amounts of anteiso-C(17 : 0), iso-C(16 : 0) and iso-C(15 : 1) were also found. All these data supported the affiliation of strain 09-Be-007(T) to the genus Promicromonospora. DNA-DNA hybridization values and physiological and biochemical data enabled strain 09-Be-007(T) to be differentiated genotypically and phenotypically from the six recognized species of the genus Promicromonospora. For these reasons, strain 09-Be-007(T) represents a novel species, for which the name Promicromonospora umidemergens sp. nov. is proposed, with 09-Be-007(T) (=DSM 22081(T)=CCM 7634(T)) as the type strain.
Martin
K
K
Institut Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, D-07745 Jena, Germany.
Schäfer
J
J
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Kämpfer
P
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D006794:Household Articles; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S