title: Symbiont succession during embryonic development of the European medicinal leech, Hirudo verbana.
abstract: The European medicinal leech, Hirudo verbana, harbors simple microbial communities in the digestive tract and bladder. The colonization history, infection frequency, and growth dynamics of symbionts through host embryogenesis are described using diagnostic PCR and quantitative PCR. Symbiont species displayed diversity in temporal establishment and proliferation through leech development.
authors: West Virginia University, Department of Biology, 53 Campus Dr. 5106 LSB, Morgantown, WV 26506, USA. rita.rio@mail.wvu.edu
authors: Rita V M
authors: RV
authors: Rio
authors: Michele
authors: M
authors: Maltz
authors: Benjamin
authors: B
authors: McCormick
authors: Alexander
authors: A
authors: Reiss
authors: Joerg
authors: J
authors: Graf
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology*; D007865:Leeches / Q000254:growth & development* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S