[Experimental analysis of microRNAs related to cardiac development differential expression].
OBJECTIVE
To investigate the differential expression profiles of microRNAs in the knockout Pax-8 mice by miRNA microarray analysis and study the function of microRNA during cardiac development.


METHODS
The knockout Pax-8 mice model was established and the total RNA derived from Pax-8 KO-/- and Pax-8 KO+/- mice heart. MicroRNA microarray containing 567 mammalian microRNA probes was used to investigate the microRNAs differential expression between Pax-8 KO-/- and Pax-8 KO+/- mice. The candidates of microRNAs were confirmed by real time RT-PCR assay.


RESULTS
The heart of pax-8 KO-/- mice became spheroidal. Left ventricle enlargement, left ventricular wall and interventricular septum thickening and papillary muscles in left ventricle enlargement were found. Furthermore, many apoptotic cells were discovered in left ventricular wall and interventricular septum in pax-8 KO-/- mice. The MicroRNA microarray result displayed 10 microRNAs differential expressions, in which 2 microRNAs became down-regulated and 8 microRNAs up-regulated by more than two folds in pax-8 KO-/- mice. This was in accordance with the result of real-time RT-PCR.


CONCLUSIONS
Some microRNAs may play important roles in cardiac development and ventricular septal defect pathogenesis.
Shi
Xiang-xiang
XX
Department of Cardiology, First Affiliated Hospital, Institute for Cardiovascular Biology and Gene, Wenzhou Medical College, Wenzhou 325000, China.
Lai
Dan-dan
DD
Zhang
Jia-ying
JY
Chu
Mao-ping
MP
Wang
Sai-bin
SB
Ni
Qiu-ming
QM
Huang
Xiao-yan
XY
Zhang
Huai-qin
HQ
Yang
De-ye
DY
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D006321:Heart / Q000254:growth & development*; D006345:Heart Septal Defects, Ventricular / Q000209:etiology / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics
D035683:MicroRNAs; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499496:Pax8 protein, mouse