title: [Expression of H5N1 avian influenza virus haemagglutinin protein in pichia pastoris by high-density cell fermentation].
abstract: We produced high pathogenic avian influenza H5N1 haemagglutinin protein HA1 in recombinant Pichia pastoris in a 10 L fermentor, to establish a high-density cell fermentation method. We studied the effects of different factors such as culture temperature, induced temperature, methanol feeding methods, trace elements on the growth of Pichia pastoris, the yield and the biologic activity of recombinant HA1 protein. The culture temperature in pre-induced and induced stage were optimized at 25 degrees C to adapt cell growth and recombinant protein expression, and induced temperature at 25 degrees C also resulted in higher biologic activity of rHA1 than at 30 degrees C. The binding activity of rHA1 against a wide-spectrum neutralizing antibody was susceptible to the presence of any trace elements, although trace elements would essentially benefit for the cell fermentation. As a conclusion, the expression level of rHA1 produced with optimized fermentation process reached 120 mg/L, which was 10.5 times higher than the one produced in regular shaking flask. The resultant high-density cell fermentation can likely produce rHA1 of H5N1 in large scale.
authors: National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Sciences, Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005, China.
authors: Kunyu
authors: K
authors: Yang
authors: Fangping
authors: F
authors: He
authors: Shaowei
authors: S
authors: Li
authors: Jiahong
authors: J
authors: Zhang
authors: Qingshan
authors: Q
authors: Lin
authors: Zhenqin
authors: Z
authors: Chen
authors: Zhongyi
authors: Z
authors: Li
authors: Jun
authors: J
authors: Zhang
authors: Ningshao
authors: N
authors: Xia
mesh_terms: D005285:Fermentation; D006389:Hemagglutinins, Viral / Q000096:biosynthesis* / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D006389:Hemagglutinins, Viral; D011994:Recombinant Proteins