title: Use of caged Nucella lapillus and Crassostrea gigas to monitor tributyltin-induced bioeffects in Irish coastal waters.
abstract: Caging studies have been previously reported to be useful for providing valuable information on biological effects of mollusks over short periods of time where resident species are absent. The degree of imposex in caged dog whelk (Nucella lapillus), was measured using the vas deferens sequence index (VSDI) and the Relative Penis Size Index (RPSI) and the extent of shell thickening in caged Pacific oyster (Crassostrea gigas) was investigated at t = 0 and t = 18 weeks. Nucella lapillus, when provided with mussels as a food source at the control site at Omey Island on the west Irish coast, did not demonstrate imposex features, whereas those transplanted to port areas did. Dunmore East exhibited the highest level of imposex (3.25 VDSI and 2.37 RPSI). Shell thickening was evident in C. gigas transplanted to Dunmore East, with low effects evident at the control location, Omey Island, and Dublin Bay at t = 18 weeks. Dry weight whole-body concentrations of organotins were most elevated in all species held at Dunmore East compared with other locations. Greatest delta15N and delta13C enrichment was observed within the tissues of the predatory N. lapillus in all three test sites. Increased assimilation in the Dublin Bay oysters might have been influenced by the presence of more nutrients at this location. Surficial sediment organotin levels were most elevated in the Dunmore East <2-mm fraction (22,707 microg tributyltin/kg dry weight), whereas low organotin levels were determined from Dublin and Omey Island sediments. The valuable application of cost-effective caging techniques to deliver integrated biological effects and chemical measurements in the absence of resident gastropod populations in potential organotin/tributyltin hotspot locations is discussed.
authors: Marine Institute, Rinville, Oranmore, County Galway, Ireland. giltrapm@tcd.ie
authors: Michelle
authors: M
authors: Giltrap
authors: Ailbhe
authors: A
authors: Macken
authors: Maria
authors: M
authors: Davoren
authors: Dan
authors: D
authors: Minchin
authors: Evin
authors: E
authors: McGovern
authors: Barry
authors: B
authors: Foley
authors: Jakob
authors: J
authors: Strand
authors: Brendan
authors: B
authors: McHugh
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000187:drug effects; D049896:Crassostrea / Q000737:chemistry / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods*; D049851:Gastropoda / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D019015:Geologic Sediments / Q000737:chemistry; D007494:Ireland; D009792:Oceans and Seas; D014220:Trialkyltin Compounds / Q000032:analysis / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin