Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater.
A novel marine, Gram-staining-negative, yellow-pigmented, rod-shaped bacterial strain, designated CNU004(T), was isolated from a seawater sample collected on the coastline of Jeju Island, South Korea. The strain was strictly aerobic, non-flagellated, non-gliding and oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CNU004(T) belongs to a distinct lineage in the family Flavobacteriaceae. Strain CNU004(T) exhibited levels of 16S rRNA gene sequence similarity of 93.8-93.9 % to its nearest phylogenetic neighbours, members of the genera Gaetbulibacter, Yeosuana and Algibacter. The new isolate required sea salts or artificial seawater for growth. The optimum ranges of temperature and pH for growth were 30-35 degrees C and pH 7.0-8.0. The DNA G+C content of strain CNU004(T) was 37.7 mol%. The major fatty acids were iso-C(15 : 0), iso-C(15 : 1) G, iso-C(17 : 0) 3-OH and iso-C(15 : 0) 3-OH. Menaquinone-6 was the major respiratory quinone. Zeaxanthin was the major carotenoid pigment produced, and flexirubin-type pigments were not produced. Strain CNU004(T) was able to degrade starch and agar. Based on its phenotypic and genotypic characteristics and on the phylogenetic evidence presented, strain CNU004(T) is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Hyunsoonleella jejuensis gen. nov., sp. nov. is proposed. The type strain of Hyunsoonleella jejuensis sp. nov. is CNU004(T) (=KCTC 22242(T) =DSM 21035(T)).
Yoon
Byoung-Jun
BJ
Department of Biology, College of Natural Science, Jeju National University, Jeju 690-756, Republic of Korea.
Lee
Dong-Heon
DH
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.|Department of Biology, College of Natural Science, Jeju National University, Jeju 690-756, Republic of Korea.
Kang
Bong-Jo
BJ
Jeju Province Fisheries Resources Research Institute, Jeju 699-915, Republic of Korea.
Kahng
Hyung-Yeel
HY
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Oh
You-Sung
YS
Department of Biology, College of Natural Science, Jeju National University, Jeju 690-756, Republic of Korea.
Sohn
Jung-Hoon
JH
Laboratory System Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Republic of Korea.
Choi
Eui-Sung
ES
Laboratory System Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Republic of Korea.
Oh
Duck-Chul
DC
Department of Biology, College of Natural Science, Jeju National University, Jeju 690-756, Republic of Korea.
D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000502:physiology; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S