title: Effects of protein concentration and detergent on endotoxin reduction by ultrafiltration.
abstract: Lipopolysaccharide (LPS), found in the outer membrane of Gram negative bacteria, only exerts its toxic effects when in free form. LPS has three major parts, lipid A, the toxic component, along with a core polysaccharide and O-specific polysaccharide. LPS monomers are known to have molecular masses between 10 to 30 kDa. Under physiological conditions, LPS exists in equilibrium between monomer and vesicle forms. LPS removal by 100 kDa ultrafiltration was more efficient (99.6% of LPS removed) with a low concentration of protein (2.0 mg/ml) compared to a high concentration (20.1 mg/ml). In the presence of different detergents (0.5% Tween 20, 1.0% taurodeoxycholate and 1.0% Triton X-100), LPS removal was more efficient at low protein concentrations (2.0 mg/ml) compared to high protein concentrations (20.1 mg/ml).
authors: 1R&D Center, Komipharm Co., Shihung, Korea.
authors: Hyun
authors: H
authors: Jang
authors: Hyo-Seung
authors: HS
authors: Kim
authors: Seung-Cheol
authors: SC
authors: Moon
authors: Young-Rae
authors: YR
authors: Lee
authors: Kang-Yeoul
authors: KY
authors: Yu
authors: Byeong-Kil
authors: BK
authors: Lee
authors: Hyun Zo
authors: HZ
authors: Youn
authors: Young-Ju
authors: YJ
authors: Jeong
authors: Byeong-Soo
authors: BS
authors: Kim
authors: Sung-Ho
authors: SH
authors: Lee
authors: Jong-Suk
authors: JS
authors: Kim
mesh_terms: D003902:Detergents / Q000494:pharmacology*; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification*; D007218:Industrial Microbiology / Q000379:methods; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification; D008970:Molecular Weight; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D014462:Ultrafiltration / Q000379:methods; D018508:Water Purification / Q000379:methods
chemical_list: D003902:Detergents; D004731:Endotoxins; D008070:Lipopolysaccharides; D011506:Proteins