Correlation analysis between mtDNA 4977-bp deletion and ageing.
Mitochondrial DNA 4977-bp deletion (common deletion) has been associated with ageing human tissues and a few studies have demonstrated their presence in breast cancer patients too; however, no previous publication evaluated both topics in the same samples group. First, when analyzing 95 breast cancer patients, we found a higher percentage of cases carrying the deletion in non-tumoral tissue 73.68% (70/95) than in the tumoral counterparts 45.26% (43/95), showing that the 4977-bp deletion is a phenomenon which commonly appear first in the non-tumoral breast tissue rather than tumoral tissue. Secondly, we analyzed and compared the ageing related distribution of the common deletion rates, in a control group of 199 samples (ages ranging from 10 to 80 years), with those found in cancer patients. Significant correlation was observed in control cases, between individual age ranges and rates of deletion (chi2: 23.21 and p<0.01). In contrast, non-tumoral breast tissue did not show a pattern of correlation, chi2: 0.042 and p: 0.837. However, interestingly, we found that the risk of having deleted mtDNAs was higher for non-tumoral breast samples than for the control group samples, OR: 2.32 [1.22-4.42, 95% CI]; this could be reflecting that other mechanisms are involved in mitochondrial genome deletion increased rate detected in breast cancer cases, than the normal ageing process.
Pavicic
Walter H
WH
IMBICE, Instituto Multidisciplinario de Biología Celular, La Plata, Argentina.
Richard
Silvina M
SM
D000293:Adolescent; D000328:Adult; D000375:Aging / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004272:DNA, Mitochondrial; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D009061:Mouth Mucosa; D017384:Sequence Deletion; D055815:Young Adult
D004272:DNA, Mitochondrial