A novel agonist anti-human OX40L monoclonal antibody that stimulates T cell proliferation and enhances cytokine secretion.
OX40L (CD252), a membrane-bound member of the tumor necrosis factor superfamily, is known to be a critical co-stimulatory molecule during immune response. Here, we describe an agonistic mouse anti-human OX40L monoclonal antibody (clone 1E7) recognizing a novel epitope of OX40L antigen. Using this antibody, we found that OX40L was transiently upregulated on CD4(+) T cells during the early stage of activation. Cross-linking of OX40L with monoclonal antibody 1E7 markedly promoted T cell proliferation and activation and enhanced cytokine production, demonstrating that OX40L transmitted a signal to T cells. Thus, OX40L may play an important role in the early phase of T cell activation and proliferation.
Wang
Qin
Q
Institute of Medical Biotechnology, Soochow University, Jiangsu, China.
Chen
Yongjing
Y
Xie
Fang
F
Ge
Yan
Y
Wang
Xuefeng
X
Zhang
Xueguang
X
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology; D000939:Epitopes; D006801:Humans; D007107:Immune System; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D053278:OX40 Ligand / Q000737:chemistry*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D003432:Cross-Linking Reagents; D016207:Cytokines; D000939:Epitopes; D053278:OX40 Ligand