Streptomyces artemisiae sp. nov., isolated from surface-sterilized tissue of Artemisia annua L.
The taxonomic position of an actinomycete strain YIM 63135(T), which was isolated from the surface-sterilized tissue of Artemisia annua L. collected from Yunnan province, south-west China, was determined by using a polyphasic approach. Morphological and chemical characteristics of the novel strain were consistent with those of the genus Streptomyces. It developed a pinkish aerial mycelium and pinkish-brown substrate mycelium on oatmeal agar. The cell wall of the strain contained ll-diaminopimelic acid. The menaquinones comprised MK-9(H(6)) (62.8 %), MK-9(H(8)) (31.4 %) and MK-9(H(4)) (5.9 %). The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, an unknown glucosamine-containing phospholipid (GluNu), phosphatidylinositol mannosides and four unknown ninhydrin-negative phospholipids. The major fatty acids were iso-C(16 : 0) (30.0 %), anteiso-C(17 : 0) (27.3 %) and anteiso-C(15 : 0) (17.0 %). The DNA G+C content of strain YIM 63135(T) was 72.6 mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain YIM 63135(T) is a member of the genus Streptomyces and exhibited 99.9 % gene sequence similarity to Streptomyces armeniacus NBRC 12555(T), while low sequence similarity values (<97.0 %) distinguished strain YIM 63135(T) from all other Streptomyces species. DNA-DNA hybridization studies suggested that strain YIM 63135(T) represents a different genomic species. On the basis of phenotypic and phylogenetic characteristics, strain YIM 63135(T) was considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces artemisiae sp. nov. is proposed, with YIM 63135(T) (=CCTCC AA 208059(T) =DSM 41953(T)) as the type strain.
Zhao
Guo-Zhen
GZ
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Li
Jie
J
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Qin
Sheng
S
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Huang
Hai-Yu
HY
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Zhu
Wen-Yong
WY
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
D031172:Artemisia annua / Q000382:microbiology*; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S