title: Determination of itopride hydrochloride by high-performance liquid chromatography with Ru(bpy)3(2+) electrogenerated chemiluminescence detection.
abstract: In this work, a stable electrogenerated chemiluminescence (ECL) detector was developed. The detector was prepared by packing cation-exchanged resin particles in a glass tube, followed by inserting Pt wires (working electrode) in this tube and sealing. The leakage of Ru(bpy)(3)(2+) can be compensated by adding a small amount of Ru(bpy)(3)(2+) into solution phase. Coupled with high-performance liquid chromatography separation, the detector has been used for determination of itopride hydrochloride in human serum. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of itopride hydrochloride in the range of 1.0 x 10(-8) g mL(-1) to 1.0 x 10(-6) g mL(-1) and the detection limit was 3 x 10(-9) g mL(-1) (S/N=3). The as-prepared ECL detector displayed good sensitivity and stability.
authors: College of Chemistry and Materials Science, Shaanxi Normal University, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Xi'an 710062, China.
authors: Yonghua
authors: Y
authors: Sun
authors: Zhujun
authors: Z
authors: Zhang
authors: Zhijun
authors: Z
authors: Xi
authors: Zuolong
authors: Z
authors: Shi
authors: Wei
authors: W
authors: Tian