The efficacy of Ankaferd Blood Stopper in antithrombotic drug-induced primary and secondary hemostatic abnormalities of a rat-bleeding model.
Ankaferd comprises a standardized mixture of plants Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum and Urtica dioica. Ankaferd Blood Stopper (ABS) as a medicinal product has been approved in the management of external hemorrhage and dental surgery bleedings in Turkey. This study aimed to evaluate the in-vivo hemostatic effect of ABS in rats pretreated with acetylsalicylic acid or enoxaparin. Wistar rats (210-270 g) of both sexes were used in this study. The animals were pretreated with acetylsalicylic acid (10 mg/kg) orally for 4 days or enoxaparin sodium (8 mg/kg) subcutaneously for 3 days or did not receive any anticoagulant before tail cut at 4th day. ABS was administered topically [a total of 4 ml (1 ml/puff x 4)] to the cut tail in the studied animals. The duration of bleeding and the amount of bleeding were measured in order to evaluate the hemostatic effect of ABS. In acetylsalicylic acid-treated animals, topical ABS reduced both the duration and also the amount of bleeding volume by 68.4 and 54.6%, respectively. It was also effective in shortening the duration of bleeding (30.6%) and decreasing the amount of bleeding (32.8%) in enoxaparin-treated animals. ABS, a traditional folkloric medicinal plant extract, has in-vivo hemostatic actions, which may provide a therapeutic potential for the management of patients with deficient hemostasis in the clinical medicine.
Kosar
Ali
A
Department of Hematology, Faculty of Medicine, University of Fatih, Istanbul, Turkey.
Cipil
Handan S
HS
Kaya
Arif
A
Uz
Burak
B
Haznedaroglu
Ibrahim C
IC
Goker
Hakan
H
Ozdemir
Oktay
O
Ercetin
Sevil
S
Kirazli
Serafettin
S
Firat
Huseyin Cahit
HC
D000704:Analysis of Variance; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D004195:Disease Models, Animal; D017984:Enoxaparin / Q000494:pharmacology; D005260:Female; D006470:Hemorrhage / Q000097:blood* / Q000188:drug therapy* / Q000209:etiology; D006487:Hemostasis / Q000187:drug effects; D006490:Hemostatics / Q000494:pharmacology*; D022125:Lacerations / Q000188:drug therapy; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000925:Anticoagulants; D017984:Enoxaparin; D006490:Hemostatics; D010936:Plant Extracts; C539751:ankaferd blood stopper; D001241:Aspirin