title: [Experimental analysis of microRNAs related to cardiac development differential expression].
abstract: OBJECTIVE
  To investigate the differential expression profiles of microRNAs in the knockout Pax-8 mice by miRNA microarray analysis and study the function of microRNA during cardiac development.
  
  
  METHODS
  The knockout Pax-8 mice model was established and the total RNA derived from Pax-8 KO-/- and Pax-8 KO+/- mice heart. MicroRNA microarray containing 567 mammalian microRNA probes was used to investigate the microRNAs differential expression between Pax-8 KO-/- and Pax-8 KO+/- mice. The candidates of microRNAs were confirmed by real time RT-PCR assay.
  
  
  RESULTS
  The heart of pax-8 KO-/- mice became spheroidal. Left ventricle enlargement, left ventricular wall and interventricular septum thickening and papillary muscles in left ventricle enlargement were found. Furthermore, many apoptotic cells were discovered in left ventricular wall and interventricular septum in pax-8 KO-/- mice. The MicroRNA microarray result displayed 10 microRNAs differential expressions, in which 2 microRNAs became down-regulated and 8 microRNAs up-regulated by more than two folds in pax-8 KO-/- mice. This was in accordance with the result of real-time RT-PCR.
  
  
  CONCLUSIONS
  Some microRNAs may play important roles in cardiac development and ventricular septal defect pathogenesis.
authors: Department of Cardiology, First Affiliated Hospital, Institute for Cardiovascular Biology and Gene, Wenzhou Medical College, Wenzhou 325000, China.
authors: Xiang-xiang
authors: XX
authors: Shi
authors: Dan-dan
authors: DD
authors: Lai
authors: Jia-ying
authors: JY
authors: Zhang
authors: Mao-ping
authors: MP
authors: Chu
authors: Sai-bin
authors: SB
authors: Wang
authors: Qiu-ming
authors: QM
authors: Ni
authors: Xiao-yan
authors: XY
authors: Huang
authors: Huai-qin
authors: HQ
authors: Zhang
authors: De-ye
authors: DY
authors: Yang
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D006321:Heart / Q000254:growth & development*; D006345:Heart Septal Defects, Ventricular / Q000209:etiology / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics
chemical_list: D035683:MicroRNAs; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499496:Pax8 protein, mouse