Human beta defensin-3 engineered keratinocyte sheets constructed by a magnetic force-based tissue engineering technique.
A multilayered keratinocyte sheet overexpressing human beta defensin-3 (HBD-3) gene was prepared by the magnetic force-based tissue engineering technique. The cell sheet demonstrated significant antimicrobial activity, indicating that the therapeutic introduction of HBD-3 gene into cell sheets may provide a new gene therapy strategy for infectious diseases.
Ito
Akira
A
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Takahashi
Tetsuya
T
Kawabe
Yoshinori
Y
Kamihira
Masamichi
M
D023181:Antimicrobial Cationic Peptides / Q000737:chemistry; D016923:Cell Death; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D008280:Magnetics; D008858:Microscopy, Phase-Contrast / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D023083:beta-Defensins / Q000378:metabolism*
D023181:Antimicrobial Cationic Peptides; C422950:DEFB103A protein, human; D012333:RNA, Messenger; D023083:beta-Defensins