Genome-wide microarray-based comparative genomic hybridization analysis of lymphoplasmacytic lymphomas reveals heterogeneous aberrations.
Lymphoplasmacytic lymphoma (LPL) is not a sharply delineated lymphoma entity, either morphologically, phenotypically, or clinically. The diagnosis is often made by excluding other small cell lymphomas with plasmacytic differentiation, thus a genetic diagnostic marker would be of great benefit. Conventional cytogenetic techniques have previously demonstrated a deletion of 6q in a proportion of cases, varying from 7 to 55%. In this report, we apply array-based comparative genomic hybridization on 11 LPL samples. Genomic aberrations were detected in 9 of 11 cases, and included gains and losses. In general, the number of genetic aberrations was relatively low (two to three abnormalities per case). Recurrent aberrations detected were deletion of 6q (two cases), deletion of chromosome 17 (two cases), gain of 3q (two cases), and gain of chromosome 7 (two cases). This report not only confirms the reported loss of 6q in a proportion of cases but also highlights the genetic heterogeneity of LPL, in accordance with the known immunophenotypical, morphological, and clinical diversity of the disease.
Buckley
Patrick G
PG
Department of Cancer Genetics, Royal College of Surgeons in Ireland, Dublin, Ireland. pbuckley@rcsi.ie
Walsh
Sarah H
SH
Laurell
Anna
A
Sundström
Christer
C
Roos
Göran
G
Langford
Cordelia F
CF
Dumanski
Jan P
JP
Rosenquist
Richard
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002874:Chromosome Mapping / Q000379:methods; D002886:Chromosomes, Human, Pair 17; D002893:Chromosomes, Human, Pair 3; D002896:Chromosomes, Human, Pair 6; D002897:Chromosomes, Human, Pair 7; D055028:Comparative Genomic Hybridization / Q000379:methods*; D005260:Female; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D008258:Waldenstrom Macroglobulinemia / Q000235:genetics* / Q000401:mortality