Nanostructured zinc oxide platform for mycotoxin detection.
Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium-tin-oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as ~5.0nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006-0.01nM/dm(3), detection limit of 0.006nM/dm(3), response time as 25s and sensitivity of 189Omega/nM/dm(3)cm(-2) with a regression coefficient of 0.997.
Ansari
Anees A
AA
Department of Science & Technology Centre on Biomolecular Electronics, New Delhi, India. aneesaansari@gmail.com
Kaushik
Ajeet
A
Solanki
Pratima R
PR
Malhotra
B D
BD
D017097:Electric Impedance; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D007074:Immunoglobulin G / Q000276:immunology; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D009183:Mycotoxins / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D009793:Ochratoxins / Q000032:analysis; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds / Q000737:chemistry*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D009183:Mycotoxins; D009793:Ochratoxins; D017971:Tin Compounds; D012710:Serum Albumin, Bovine; C109984:indium tin oxide; D015034:Zinc Oxide