title: Fluorescence cross sections of bioaerosols and suspended biological agents.
abstract: Laser-induced fluorescence is used to investigate fluorescence properties of unwashed Bacillus thuringiensis and Bacillus subtilis spores, ovalbumin, and washed bacteriophage MS2. A fluorescence detector is calibrated to obtain absolute fluorescence cross sections. Fluorescence maps of biological aerosols and suspensions are measured at a wide excitation range from 210 to 419 nm and a wide detection range from 315 to 650 nm. The dominant features of the measured spectra are the amino acid peaks, having excitation maxima at 220 and 280 nm. The peaks are similar for the bacterial spores, both for aerosols and suspensions, whereas the peaks are shifted toward the shorter emission wavelengths for the suspended ovalbumin and MS2. Moreover, the fluorescence emission, excited above 320 nm is more intensive for the aerosols than the suspensions.
authors: Optics Laboratory, Department of Physics, Tampere University of Technology, P.O. Box 692, FI-33101 Tampere, Finland. albert.manninen@tut.fi
authors: Albert
authors: A
authors: Manninen
authors: Matti
authors: M
authors: Putkiranta
authors: Jaakko
authors: J
authors: Saarela
authors: Antti
authors: A
authors: Rostedt
authors: Tapio
authors: T
authors: Sorvajärvi
authors: Juha
authors: J
authors: Toivonen
authors: Marko
authors: M
authors: Marjamäki
authors: Jorma
authors: J
authors: Keskinen
authors: Rolf
authors: R
authors: Hernberg
mesh_terms: D000336:Aerosols; D000391:Air Microbiology; D001412:Bacillus subtilis / Q000378:metabolism*; D001413:Bacillus thuringiensis / Q000378:metabolism*; D001435:Bacteriophages / Q000378:metabolism; D004867:Equipment Design; D005453:Fluorescence; D055095:Optics and Photonics; D010047:Ovalbumin / Q000737:chemistry; D010316:Particle Size; D013050:Spectrometry, Fluorescence / Q000379:methods; D013171:Spores, Bacterial / Q000378:metabolism
chemical_list: D000336:Aerosols; D010047:Ovalbumin