Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples.
A highly sensitive, specific, simple, and rapid chemiluminescence enzyme immunoassay (CLEIA) was developed for the determination of microcystin-LR (MC-LR). Several physicochemical parameters such as the chemiluminescent assay mediums, the dilution ratio of MC-LR-OVA conjugate, monoclonal antibody concentration, and peroxidase labeled antibody concentration were studied and optimized. Under optimum conditions, calibration curve obtained for MC-LR had detection limits of 0.032+/-0.003 microg L(-1), the 50% inhibition concentration (IC50) was 0.20+/-0.02 microg L(-1) and the quantitative detection range was 0.062-0.65 microg L(-1). The proposed methods was successfully applied to the monitoring of MC-LR in spiked water samples without significant effect of the matrix, and the recovery of MC-LR added to water samples at different concentrations ranged from 80% to 115% with the coefficients of variation (CVs) less than 9%. The LOD attained from the calibration curves and the results obtained for the real samples demonstrate the potential use of CLEIA as a screening tool for the analysis of MC-LR in environmental samples.
Long
F
F
Environmental Simulate and Pollution Control State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China. longf04@mails.tsinghua.edu.cn
Shi
H C
HC
He
M
M
Sheng
J W
JW
Wang
J F
JF
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D004784:Environmental Monitoring; D007124:Immunoenzyme Techniques / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D014867:Water / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; D014867:Water; C057862:cyanoginosin LR