In vitro precursor-directed synthesis of polyketide analogues with coenzyme a regeneration for the development of antiangiogenic agents.
Polyketide analogues are produced via in vitro reconstruction of a precursor-directed polyketide biosynthetic pathway. Malonyl-CoA synthetase (MCS) was used in conjunction with chalcone synthase (CHS), thereby allowing efficient use of synthetic starter molecules and malonate as extender. Coenzyme-A was recycled up to 50 times. The use of a simple immobilization procedure resulted in up to a 30-fold higher yield of pyrone CHS products than that obtained with the free enzyme solutions.
Kim
Moon Il
MI
Department of Chemical and Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Kwon
Seok Joon
SJ
Dordick
Jonathan S
JS
D000217:Acyltransferases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000737:chemistry; D020533:Angiogenesis Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000378:metabolism*; D001688:Biological Products / Q000138:chemical synthesis* / Q000737:chemistry; D002384:Catalysis; D003065:Coenzyme A / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000378:metabolism*; D018942:Macrolides / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D011753:Pyrones
D020533:Angiogenesis Inhibitors; D001426:Bacterial Proteins; D001688:Biological Products; D018942:Macrolides; D011753:Pyrones; D000255:Adenosine Triphosphate; D000217:Acyltransferases; C020141:flavanone synthetase; D003066:Coenzyme A Ligases; C021533:malonyl-CoA synthetase; D003065:Coenzyme A