title: Tissue expression and subcellular localization of Mipu1, a novel myocardial ischemia-related gene.
abstract: Myocardial ischemic preconditioning up-regulated protein 1 (Mipu1), a novel zinc finger protein, was originally cloned using bioinformatic analysis and 5' RACE technology of rat heart after a transient myocardial ischemia/reperfusion procedure in our laboratory. In order to investigate the functions of Mipu1, the recombinant prokaryotic expression vector pQE31-Mipu1 was constructed and transformed into Escherichia coli M15(pREP4), and Mipu1-6His fusion protein was expressed and purified. The identity of the purified protein was confirmed by mass spectrometry. The molecular mass of the Mipu1 protein was 70.03779 kDa. The fusion protein was intracutaneously injected to immunize New Zealand rabbits to produce a polyclonal antibody. The antibody titer was approximately 1:16,000. The antibody was tested by Western blotting for specificity and sensitivity. Using the antibody, it was found that Mipu1 was highly expressed in the heart and brain of rats and was localized in the nucleus of H9c2 myogenic cells. The present study lays the foundation for further study of the biological functions of Mipu1.
authors: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
authors: G
authors: G
authors: Wang
authors: X
authors: X
authors: Zuo
authors: L
authors: L
authors: Jiang
authors: K
authors: K
authors: Wang
authors: X
authors: X
authors: Wei
authors: B
authors: B
authors: Zhang
authors: X
authors: X
authors: Xiao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D017202:Myocardial Ischemia / Q000235:genetics*; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000737:chemistry*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; C520710:Mipu1 protein, rat; D009687:Nuclear Proteins; D012097:Repressor Proteins