Systematic antigenic profiling of hematopoietic antigens on ovarian carcinoma cells identifies membrane proteins for targeted therapy development.
OBJECTIVE
We studied ovarian cancers for the expression of membrane markers of hematopoietic origin.


METHODS
We used flow cytometry to systematically characterize the expression of more than 30 hematologic antigens on ovarian carcinoma cell lines and to assess their stability under estrogen exposure. The expression of the antigens was validated by a bioinformatics survey and immunohistochemical staining of ovarian cancer specimens.


RESULTS
Several antigens were expressed by the majority of the cells, such as CD15, CD71, and CD138, whereas others were found on small and distinct cellular subpopulations. The expression patterns of the different markers were unaffected by estrogen exposure, indicating their stability.


CONCLUSIONS
The antigens described in our work may serve as potential targets for new and existing targeted drugs.
Helpman
Limor
L
Department of Obstetrics and Gynecology, Tel Aviv Sourasky Medical Center, Tel-Aviv, Israel.
Katz
Ben-Zion
BZ
Safra
Tamar
T
Schreiber
Letizia
L
Levine
Zurit
Z
Nemzer
Sergey
S
Kinar
Yaron
Y
Grisaru
Dan
D
D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D019295:Computational Biology; D015195:Drug Design; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006410:Hematopoiesis; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000628:therapy*; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism; D053668:Syndecan-1 / Q000235:genetics / Q000378:metabolism
D015703:Antigens, CD; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; C068126:CD71 antigen; D016256:Lewis X Antigen; D011990:Receptors, Transferrin; C508241:SDC1 protein, human; D053668:Syndecan-1