title: Mannose-binding lectin is present in human semen and modulates cellular adhesion of Neisseria gonorrhoeae in vitro.
abstract: Mannose-binding lectin (MBL) is an innate immune molecule present in blood and some mucosal tissues, which can influence microbial attachment and inflammatory responses of host cells during infection. In this study MBL was found to be present at a low concentration in semen samples in the range 1.2-24.9 ng/ml. Co-incubation of bacteria with semen resulted in the binding of MBL to the bacterial surface. Neisseria gonorrhoeae is a common cause of genitourinary infection. MBL bound to N. gonorrhoeae with strain-to-strain variation in the intensity of binding and nature of the bacterial receptor. Pretreatment with MBL concentrations similar to those found in human serum modulated the adhesion of N. gonorrhoeae strain FA1090 but not strain MS11 to epithelial cells. This effect was dose-dependent. This work demonstrates that MBL is present in human semen and modifies cellular responses to N. gonorrhoeae in a concentration-dependent manner.
authors: Academic Unit of Infection and Immunity, University of Sheffield Medical School, Sheffield S10 2RX, UK. j.b.wing@shef.ac.uk
authors: J B
authors: JB
authors: Wing
authors: D L
authors: DL
authors: Jack
authors: M E
authors: ME
authors: Lee
authors: A A
authors: AA
authors: Pacey
authors: G R
authors: GR
authors: Kinghorn
authors: R C
authors: RC
authors: Read
mesh_terms: D001422:Bacterial Adhesion; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D006069:Gonorrhea / Q000276:immunology*; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000032:analysis / Q000378:metabolism*; D009344:Neisseria gonorrhoeae; D012661:Semen / Q000737:chemistry / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D016207:Cytokines; D037601:Mannose-Binding Lectin