Clinical pathology alterations in pregnant and non-pregnant rats following scorpion envenomation.
Scorpion envenomation is a growing problem in many countries, especially among women and children. Existing diagnostic criteria are not sufficiently specific to allow antivenin administration in the absence of a confirmed scorpion sting. This study was performed to evaluate conventional haematological and serum chemical measurements as potential indices of scorpion envenomation. Adult, cycling nulliparous and near-term primiparous, white Wistar rats received a single subcutaneous injection of crude venom (600 µg/kg) from the Buthidae scorpion (Buthus occitanus tunetanus). All envenomed rats were observed for external signs and symptoms of toxicity until necropsy, which entailed terminal blood collection at either 0.5, 1, 2, or 4 hr after venom administration (n = 6 per reproductive state per time-point) for evaluation of selected clinical chemistry and haematological analytes. Control cohorts (matched for age and reproductive state) received saline injections subcutaneously and were necropsied at 0.5 hr. Almost all envenomed rats but no control animals displayed physical symptoms of intoxication, including agitation, mastication with hypersalivation, and/or vocalizing. Reproducible alterations in clinical pathology parameters were lacking in venom-treated rats regardless of reproductive status, although modest but significant Rho correlations suggested that mild haemoconcentration, haemolysis, renal function deficits and possibly coagulation difficulties developed over time.
Nasr
Hmed Ben
HB
Laboratory of Pharmacology, Medical School of Sfax, Sfax, Tunisia. hmedbnasr@gmail.com
Bolon
Brad
B
Hammami
Serria Turky
ST
Sahnoun
Zouhier
Z
Jamoussi
Kamel
K
Lahyani
Amina
A
Zeghal
Khaled Mounir
KM
D000818:Animals; D000997:Antivenins / Q000627:therapeutic use; D001786:Blood Glucose / Q000032:analysis; D004195:Disease Models, Animal; D004573:Electrolytes / Q000097:blood; D005260:Female; D006403:Hematologic Tests / Q000379:methods; D010338:Pathology, Clinical; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012604:Scorpion Venoms / Q000008:administration & dosage* / Q000097:blood* / Q000633:toxicity*; D012605:Scorpions / Q000737:chemistry
D000997:Antivenins; D001786:Blood Glucose; D004573:Electrolytes; D012604:Scorpion Venoms