Comparative study of in situ hybridization and immunohistochemistry for the detection of porcine circovirus 2 in formalin-fixed, paraffin-embedded tissues.
In situ hybridization and immunohistochemistry with different types of antibody (monoclonal vs. polyclonal, natural vs. synthetic) was compared to detect porcine circovirus 2 (PCV2) in formalin-fixed, paraffin-embedded tissues from pigs with experimentally and naturally occurring postweaning multisystemic wasting syndrome. PCV2 DNA and antigen was detected in tissues from both experimentally and naturally infected pigs by in situ hybridization and immunohistochemistry, respectively. Statistical evaluation revealed that more PCV2 positive signals were significantly detected in both experimentally and naturally infected pigs by in situ hybridization compared with immunohistochemistry (P<0.05). The results of this study demonstrated that in situ hybridization proved more sensitive than immunohistochemistry for the detection of PCV2 in formalin-fixed, paraffin-embedded lymph node tissues.
Kim
Duyeol
D
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
Ha
Yooncheol
Y
Lee
Yong-Hoon
YH
Chae
Sungwon
S
Lee
Kichan
K
Han
Kiwon
K
Kim
Junghyun
J
Lee
Ju-Ho
JH
Kim
Sung-Hoon
SH
Hwang
Kyu-Kye
KK
Chae
Chanhee
C
D000818:Animals; D017925:Circovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D007150:Immunohistochemistry / Q000662:veterinary*; D017403:In Situ Hybridization / Q000662:veterinary*; D008198:Lymph Nodes / Q000821:virology*; D016612:Paraffin Embedding; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
D004279:DNA, Viral