title: Association of a novel polymorphism in the bovine PPARGC1A gene with growth, slaughter and meat quality traits in Brangus steers.
abstract: The PPARGC1A gene (peroxysome proliferator-activated receptor-gamma coactivator 1alpha gene) controls muscle fiber type and brown adipocyte differentiation; therefore, it is a candidate gene for beef quality traits (tenderness and fat content). Two SNPs (Single Nucleotide Polymorphisms) were identified within exon 8 by multiple alignment of DNA sequences obtained from 24 bulls: a transition G/A (SNP 1181) and a transversion A/T (SNP 1299). The SNP 1181 is a novel SNP, corresponding to a non-conservative substitution (AGT/AAT) that could be the cause of amino acid substitution ((364)Serine/(364)Asparagine). A Mismatch PCR method was designed to determine genotypes of 73 bulls and 268 steers for SNP 1181. Growth, slaughter and meat quality information were available for the group of steers. Allele A of SNP 1181 was not found in Angus. In 243 steers, no significant differences (P > 0.05) were found for either final live body weight, gain in backfat thickness in Spring, kidney fat weight, kidney fat percentage, Warner-Bratzler shear force at 7 days postmortem, intramuscular fat percentage or meat colour between genotype GG and AG. This SNP could be included in breed composition and population admixture analyses because there are marked differences in allelic frequencies between Bos taurus and Bos indicus breeds.
authors: Area Genética, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Chorroarín 280, (1427) Ciudad Autónoma de Buenos Aires, Argentina. lsoria@fvet.uba.ar
authors: L A
authors: LA
authors: Soria
authors: P M
authors: PM
authors: Corva
authors: A
authors: A
authors: Branda Sica
authors: E L
authors: EL
authors: Villarreal
authors: L M
authors: LM
authors: Melucci
authors: C A
authors: CA
authors: Mezzadra
authors: J
authors: J
authors: Papaleo Mazzucco
authors: G
authors: G
authors: Fernández Macedo
authors: C
authors: C
authors: Silvestro
authors: A
authors: A
authors: Schor
authors: M C
authors: MC
authors: Miquel
mesh_terms: D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics; D002417:Cattle / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D008460:Meat / Q000592:standards; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1