Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil.
Two novel yeast species, Candida aechmeae sp. nov. and Candida vrieseae sp. nov., were isolated from bromeliads in Itapuã Park, Rio Grande do Sul, Brazil. These species are genetically isolated from all other currently recognized ascomycetous yeasts based on their sequence divergence in the D1/D2 domain of the LSU rRNA gene. C. aechmeae sp. nov. is phylogenetically close to Candida ubatubensis, a species also isolated from bromeliads in Brazil, but the novel species can be differentiated on the basis of differences in the D1/D2 domain and positive results for the assimilation of l-arabinose, raffinose, inulin and citrate. Candida vrieseae sp. nov. is phylogenetically placed in a clade near Candida membranifaciens that is composed of several species associated with insects, but the novel species can be differentiated from them by the D1/D2 and ITS gene sequences, positive results for the assimilation of nitrite and a negative result for the assimilation of ethylamine. The type strain for Candida aechmeae sp. nov. is BI153(T) (=CBS 10831(T)=NRRL Y-48456(T)) and the type strain for C. vrieseae sp. nov. is BI146(T) (=CBS 10829(T)=NRRL Y-48461(T)).
Landell
Melissa Fontes
MF
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.|Departamento de Microbiologia, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
Billodre
Raisa
R
Departamento de Microbiologia, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
Ramos
Jesus P
JP
Fundação Oswaldo Cruz, Rio de Janeiro RJ, Brazil.
Leoncini
Orílio
O
Instituto do Câncer, Rio de Janeiro RJ, Brazil.
Vainstein
Marilene H
MH
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.|Departamento de Microbiologia, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
Valente
Patrícia
P
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.|Departamento de Microbiologia, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
D001938:Brazil; D031231:Bromelia / Q000382:microbiology*; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S