Expression of genes involved in redox homeostasis and antioxidant defense in a marine macroalga Ulva fasciata by excess copper.
The expression of genes involved in the control of redox homeostasis and antioxidant defense was studied in macroalga Ulva fasciata Delile in response to 5 and 50 microM CuSO(4). Redox-related genes, methionine sulfoxide reductase A (UfMsrA), thioredoxin (UfTrx), cyclophilin (UfCyp), and ferritin (UfFer) that were up-regulated by excess Cu [Wu, T.M., Lee, T.M., 2008. Regulation of activity and gene expression of antioxidant enzymes in Ulva fasciata Delile (Ulvales, Chlorophyta) in response to excess copper. Phycologia 47, 346-360] were cloned and their expression was compared to superoxide dismutase (UfMnsod and UfFesod), ascorbate peroxidase (UfApx), glutathione reductase (UfGr), and catalase (UfCat). Transcripts of UfMsrA, UfCyp, and UfFer were increased by excess Cu with a peak at 3h and that of UfTrx increased after 6-9h, but not affected by 4-day exposure to excess Cu, except an increase in UfMsrA transcript. Transcripts of UfMnsod, UfFesod, UfApx, UfGr and UfCat can be increased by 4-day exposure to Cu excess [Wu, T.M., Lee, T.M., 2008. Regulation of activity and gene expression of antioxidant enzymes in Ulva fasciata Delile (Ulvales, Chlorophyta) in response to excess copper. Phycologia 47, 346-360] but not by short-term excess Cu treatment, except UfGr whose transcript increased after 3h. Reactive oxygen species involved in up-regulation of antioxidant defense enzymes genes. These results suggest that the expression of genes of antioxidant defense enzymes and UfMsrA are associated with long-term adaptation of U. fasciata to Cu excess and transcription of redox-related genes and UfGr is up-regulated for short-term acclimation.
Wu
Tsung-Meng
TM
Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Hsu
Yuan-Ting
YT
Sung
Ming-Shiuan
MS
Hsu
Yi-Ting
YT
Lee
Tse-Min
TM
D000975:Antioxidants / Q000378:metabolism*; D003300:Copper / Q000633:toxicity*; D019327:Copper Sulfate / Q000378:metabolism / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects* / Q000502:physiology; D006706:Homeostasis / Q000187:drug effects* / Q000235:genetics*; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D044445:Ulva / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D003300:Copper; D013482:Superoxide Dismutase; D019327:Copper Sulfate