Increased isoprostane levels in oleic acid-induced lung injury.
The present study was performed to examine a role of oxidative stress in oleic acid-induced lung injury model. Fifteen anesthetized sheep were ventilated and instrumented with a lung lymph fistula and vascular catheters for blood gas analysis and measurement of isoprostanes (8-epi prostaglandin F2alpha). Following stable baseline measurements, oleic acid (0.08 ml/kg) was administered and observed 4 h. Isoprostane was measured by gas chromatography mass spectrometry with the isotope dilution method. Isoprostane levels in plasma and lung lymph were significantly increased 2 h after oleic acid administration and then decreased at 4 h. The percent increases in isoprostane levels in plasma and lung lymph at 2 h were significantly correlated with deteriorated oxygenation at the same time point, respectively. These findings suggest that oxidative stress is involved in the pathogenesis of the pulmonary fat embolism-induced acute lung injury model in sheep and that the increase relates with the deteriorated oxygenation.
Ono
Koichi
K
Department of Anesthesiology and Resuscitation, Shinshu University School of Medicine, Matsumoto, Japan.
Koizumi
Tomonobu
T
Tsushima
Kenji
K
Yoshikawa
Sumiko
S
Yokoyama
Toshiki
T
Nakagawa
Rikimaru
R
Obata
Toru
T
D055371:Acute Lung Injury / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D028421:Isoprostanes / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D019301:Oleic Acid / Q000633:toxicity*; D012756:Sheep
D028421:Isoprostanes; D019301:Oleic Acid