Steroidal glycosides from Agave utahensis and their cytotoxic activity.
Eight new spirostanol saponins (1-8) and three new furostanol saponins (9-11) were isolated from the whole plants of Agave utahensis. The structures of 1-11 were determined by analysis of extensive spectroscopic data. The saponins were evaluated for their cytotoxic activity against HL-60 human promyelocytic leukemia cells. Compound 1 showed cytotoxicity against HL-60 cells with an IC(50) value of 4.9 microg/mL, induced apoptosis in HL-60 cells, and markedly activated caspase-3.
Yokosuka
Akihito
A
Laboratory of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Horinouchi 1432-1, Hachiouji, Tokyo 192-0392, Japan. yokosuka@toyaku.ac.jp
Jitsuno
Maki
M
Yui
Satoru
S
Yamazaki
Masatoshi
M
Mimaki
Yoshihiro
Y
D027882:Agave / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D053148:Caspase 3 / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013237:Stereoisomerism
D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D013150:Spirostans; D053148:Caspase 3