Epilithonimonas lactis sp. nov., isolated from raw cow's milk.
A Gram-staining-negative, rod-shaped, oxidase-positive, aerobic, non-motile and yellow-pigmented bacterial strain containing flexirubin type pigments, designated H1(T), was isolated from raw cow's milk in Israel. 16S rRNA gene sequence analysis indicated that the isolate should be placed in the genus Epilithonimonas (family Flavobacteriaceae, phylum Bacteroidetes). The level of 16S rRNA gene sequence similarity between strain H1(T) and the type strain of Epilithonimonas tenax was 97.6 %. Strain H1(T) grew at 5-33 degrees C and with 0-2.0 % NaCl. The dominant cellular fatty acids of strain H1(T) were iso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1) omega7c), and the DNA G+C content was 38.0 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the milk isolate is classified as a new species in the genus Epilithonimonas , for which the name Epilithonimonas lactis sp. nov. (type strain H1(T) =LMG 24401(T) =DSM 19921(T)) is proposed.
Shakéd
Tamar
T
Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mount Carmel, Haifa 31905, Israel.
Hantsis-Zacharov
Elionora
E
Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mount Carmel, Haifa 31905, Israel.
Halpern
Malka
M
Department of Biology Education, Faculty of Science and Science Education, University of Haifa, Oranim, Tivon 36006, Israel.|Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mount Carmel, Haifa 31905, Israel.
D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S