Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., to accommodate Acinetobacter genomic species 10 and 11, respectively.
Acinetobacter genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA-DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP) analysis rpoB sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA-DNA reassociation. Results indicate that A. bereziniae and A. guillouiae represent two phenetically and phylogenetically distinct groups within the genus Acinetobacter. Based on the comparative analysis of housekeeping genes (16S rRNA and rpoB genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize d-glucose and to grow at 38 degrees C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, A. bereziniae strains were positive for both tests, whereas A. guillouiae strains were negative in these tests. The strains of A. bereziniae originated mainly from human clinical specimens, whereas A. guillouiae strains were isolated from different environmental sources in addition to human specimens. The type strain of A. bereziniae sp. nov. is LMG 1003(T) (=CIP 70.12(T) =ATCC 17924(T)) and that of A. guillouiae sp. nov. is LMG 988(T) (=CIP 63.46( T) =ATCC 11171(T) =CCUG 2491(T)).
Nemec
Alexandr
A
Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, CZ-100 42 Prague 10, Czech Republic.
Musílek
Martin
M
National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Šrobárova 48, CZ-100 42 Prague 10, Czech Republic.
Šedo
Ondrej
O
Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5 (Building A2), CZ-625 00 Brno, Czech Republic.
De Baere
Thierry
T
Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, 3 Blok A, De Pintelaan 185, B-9000 Gent, Belgium.
Maixnerová
Martina
M
Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, CZ-100 42 Prague 10, Czech Republic.
van der Reijden
Tanny J K
TJK
Department of Infectious Diseases, Leiden University Medical Center C5-P, PO Box 9600, 2300 RC Leiden, The Netherlands.
Zdráhal
Zbyněk
Z
Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5 (Building A2), CZ-625 00 Brno, Czech Republic.
Vaneechoutte
Mario
M
Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, 3 Blok A, De Pintelaan 185, B-9000 Gent, Belgium.
Dijkshoorn
Lenie
L
Department of Infectious Diseases, Leiden University Medical Center C5-P, PO Box 9600, 2300 RC Leiden, The Netherlands.
D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D000151:Acinetobacter Infections / Q000382:microbiology*; D054458:Amplified Fragment Length Polymorphism Analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D020459:Genes, rRNA; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014946:Wound Infection / Q000382:microbiology*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage