title: Optical detection of specific genes for genetically modified soybean and maize using multiplex PCR coupled with primer extension on a plastic plate.
abstract: A novel DNA microarray method to detect one line of genetically modified (GM) soybean and five lines of GM maize was developed using multiplex PCR coupled with primer extension on a plastic plate. Multiplex PCR products were applied on an extension primer-immobilized plate and the spots corresponding to the DNA sequences were visualized. This method is a rapid and simple way to detect GM soybean and GM maize optically.
authors: School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan. harikai@mukogawa-u.ac.jp
authors: Naoki
authors: N
authors: Harikai
authors: Shin
authors: S
authors: Saito
authors: Midori
authors: M
authors: Abe
authors: Kazunari
authors: K
authors: Kondo
authors: Kazumi
authors: K
authors: Kitta
authors: Hiroshi
authors: H
authors: Akiyama
authors: Reiko
authors: R
authors: Teshima
authors: Kenji
authors: K
authors: Kinoshita
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D030841:Food, Genetically Modified; D017343:Genes, Plant / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D055620:Optical Phenomena; D030821:Plants, Genetically Modified; D010969:Plastics; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics*; D013997:Time Factors; D003313:Zea mays / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D010969:Plastics