Evaluation of seminal citrate level by 1H nuclear magnetic resonance spectroscopy in men with spinal cord injury.
METHODS
A case-control evaluating seminal citrate in patients with spinal cord injury (SCI).


OBJECTIVE
Several studies have shown neurological prostatic dysfunction in patients with SCI, as confirmed by low levels of seminal prostate-specific antigen (PSA), which is used as a parameter of gland activity. However, seminal citrate, produced almost exclusively by the prostate, could also be used as a marker of prostate function. Thus, the objective of this study was to determine whether SCI causes any changes in seminal citrate concentration and to compare the results obtained for patients and healthy men (controls).


METHODS
The study was carried out in Brazil.


METHODS
We studied 30 men with SCI aged on average 37.77+/-10.04 years and 30 controls aged on average 38.03+/-10.06 years. Blood and semen samples were collected after 3 days of abstinence from ejaculation. Fifteen minutes after collection, semen was stored in liquid nitrogen and the samples were submitted to (1)H nuclear magnetic resonance ((1)H NMR). Serum was stored at a controlled temperature of -70 to -79 degrees C and later used for the determination of testosterone, prolactin and total PSA using an AxSYM instrument and Abbott reagents.


RESULTS
The median concentration of seminal citrate was significantly lower in patients than in controls (521.65 versus 858.30 mg per 100 ml, P<0.001).


CONCLUSIONS
Patients with SCI have a significant reduction of seminal citrate as a consequence of neurological dysfunction of the prostate.
Alexandrino
A P
AP
Disciplina de Urologia, Departamento de Clínica Cirúrgica-UEL, Londrina, Paraná, Brasil. m.aurelio@sercomtel.com.br
Rodrigues
M A F
MA
Matsuo
T
T
Schuquel
I T A
IT
Costa
W F
WF
Santilli
J C
JC
D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D019343:Citric Acid / Q000032:analysis / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology; D006801:Humans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011469:Prostatic Diseases / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology*; D012661:Semen / Q000737:chemistry / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012735:Sexual Dysfunction, Physiological / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000150:complications*
D015415:Biomarkers; D019343:Citric Acid; D006859:Hydrogen