title: Establishment of an indicator cell line for monitoring bovine immunodeficiency virus infection and inhibitor susceptibility.
abstract: Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BIV long terminal repeat was constructed. The BIV indicator cell line, designated BIVE, can detect BIV infection more easily and effectively than the established method, which involves the observation of cell cytopathic effects. Furthermore, viral titration using an assay based on the indicator cells is 100 times more sensitive than the assay based on cytopathic effect. The finding that BIV can infect the hamster cell line expands the known host range of BIV in vitro. The BIV indicator cell line could also be used for the evaluation of the inhibitory effect of antiviral agents. The fusion inhibition effect of the heptad repeat 2 region of the BIV envelope protein could also be quantified.
authors: Key Laboratory of Molecular Microbiology and Biotechnology, Ministry of Education, Tianjin, China.
authors: Xue
authors: X
authors: Yao
authors: Yang
authors: Y
authors: Su
authors: Chang
authors: C
authors: Liu
authors: Juan
authors: J
authors: Tan
authors: Li
authors: L
authors: Liu
authors: Yun-Qi
authors: YQ
authors: Geng
authors: Wen-Tao
authors: WT
authors: Qiao
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics; D016087:Immunodeficiency Virus, Bovine / Q000187:drug effects / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity
chemical_list: D000998:Antiviral Agents; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins