title: Controlled intracellular release of peptides from microcapsules enhances antigen presentation on MHC class I molecules.
abstract: To understand the time course of action of any small molecule inside a single cell, one would deposit a defined amount inside the cell and initiate its activity at a defined moment. An elegant way to achieve this is to encapsulate the molecule in a micrometer-sized reservoir, introduce it into a cell, remotely open its wall by a laser pulse, and then follow the biological response by microscopy. The validity of this approach is validated here using microcapsules with defined walls that are doped with metallic nanoparticles so as to enable them to be opened with an infrared laser. The capsules are loaded with a fluorescent antigenic peptide and introduced into mammalian cultured cells where, upon laser-induced release, the peptide binds to major histocompatibility complex (MHC) class I proteins and elicits their cell surface transport. The concept of releasing a drug inside a cell and following its action is applicable to many problems in cell biology and medicine.
authors: Biophysics, Jacobs University Bremen, 28759 Bremen, Germany.
authors: Raghavendra
authors: R
authors: Palankar
authors: André G
authors: AG
authors: Skirtach
authors: Oliver
authors: O
authors: Kreft
authors: Matthieu
authors: M
authors: Bédard
authors: Malgorzata
authors: M
authors: Garstka
authors: Keith
authors: K
authors: Gould
authors: Helmuth
authors: H
authors: Möhwald
authors: Gleb B
authors: GB
authors: Sukhorukov
authors: Matthias
authors: M
authors: Winterhalter
authors: Sebastian
authors: S
authors: Springer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001692:Biological Transport; D016466:CHO Cells; D002214:Capsules; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003911:Dextrans / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D042541:Intracellular Space / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011108:Polymers / Q000737:chemistry; D013997:Time Factors; D014709:Vero Cells
chemical_list: D002214:Capsules; D003911:Dextrans; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D011108:Polymers; D049452:Green Fluorescent Proteins