title: Light scattering characterization of mitochondrial aggregation in single cells.
abstract: Three dimensional finite-difference time-domain (FDTD) simulations are employed to show that light scattering techniques may be used to infer the mitochondrial distributions that exist within single biological cells. Two-parameter light scattering plots of the FDTD light scattering spectra show that the small angle forward scatter can be used to differentiate the case of a random distribution of mitochondria within a cell model from that in which the mitochondria are aggregated to the nuclear periphery. Fourier transforms of the wide angle side scatter spectra show a consistent highest dominant frequency, which may be used for size differentiation of biological cells with distributed mitochondria.
authors: Department of Physics, University of Alberta, Edmonton T6G 2G7, Canada. xtsu@phys.ualberta.ca
authors: Xuan-Tao
authors: XT
authors: Su
authors: Kirat
authors: K
authors: Singh
authors: Wojciech
authors: W
authors: Rozmus
authors: Christopher
authors: C
authors: Backhouse
authors: Clarence
authors: C
authors: Capjack
mesh_terms: D002449:Cell Aggregation / Q000502:physiology; D002452:Cell Count / Q000379:methods*; D003198:Computer Simulation; D008027:Light; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure*; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012542:Scattering, Radiation