title: Genomic analysis reveals few genetic alterations in pediatric acute myeloid leukemia.
abstract: Pediatric de novo acute myeloid leukemia (AML) is an aggressive malignancy with current therapy resulting in cure rates of only 60%. To better understand the cause of the marked heterogeneity in therapeutic response and to identify new prognostic markers and therapeutic targets a comprehensive list of the genetic mutations that underlie the pathogenesis of AML is needed. To approach this goal, we examined diagnostic leukemic samples from a cohort of 111 children with de novo AML using single-nucleotide-polymorphism microarrays and candidate gene resequencing. Our data demonstrate that, in contrast to pediatric acute lymphoblastic leukemia (ALL), de novo AML is characterized by a very low burden of genomic alterations, with a mean of only 2.38 somatic copy-number alterations per leukemia, and less than 1 nonsynonymous point mutation per leukemia in the 25 genes analyzed. Even more surprising was the observation that 34% of the leukemias lacked any identifiable copy-number alterations, and 28% of the leukemias with recurrent translocations lacked any identifiable sequence or numerical abnormalities. The only exception to the presence of few mutations was acute megakaryocytic leukemias, with the majority of these leukemias being characterized by a high number of copy-number alterations but rare point mutations. Despite the low overall number of lesions across the patient cohort, novel recurring regions of genetic alteration were identified that harbor known, and potential new cancer genes. These data reflect a remarkably low burden of genomic alterations within pediatric de novo AML, which is in stark contrast to most other human malignancies.
authors: Department of Pathology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.
authors: Ina
authors: I
authors: Radtke
authors: Charles G
authors: CG
authors: Mullighan
authors: Masami
authors: M
authors: Ishii
authors: Xiaoping
authors: X
authors: Su
authors: Jinjun
authors: J
authors: Cheng
authors: Jing
authors: J
authors: Ma
authors: Ramapriya
authors: R
authors: Ganti
authors: Zhongling
authors: Z
authors: Cai
authors: Salil
authors: S
authors: Goorha
authors: Stanley B
authors: SB
authors: Pounds
authors: Xueyuan
authors: X
authors: Cao
authors: Caroline
authors: C
authors: Obert
authors: Jianling
authors: J
authors: Armstrong
authors: Jinghui
authors: J
authors: Zhang
authors: Guangchun
authors: G
authors: Song
authors: Raul C
authors: RC
authors: Ribeiro
authors: Jeffrey E
authors: JE
authors: Rubnitz
authors: Susana C
authors: SC
authors: Raimondi
authors: Sheila A
authors: SA
authors: Shurtleff
authors: James R
authors: JR
authors: Downing
mesh_terms: D002648:Child; D005260:Female; D018628:Gene Dosage; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D016547:Kinesins / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D009154:Mutation; D009218:Myosins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011518:Proto-Oncogene Proteins / Q000235:genetics; D062085:RNA, Long Noncoding; D000075142:RUNX1 Translocation Partner 1 Protein; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: C095516:AFDN protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011518:Proto-Oncogene Proteins; D062085:RNA, Long Noncoding; D000075142:RUNX1 Translocation Partner 1 Protein; C082222:RUNX1T1 protein, human; D014157:Transcription Factors; C000613877:long noncoding RNA CCDC26, human; D009218:Myosins; D016547:Kinesins