title: Chronic stress affects the expression of brain-derived neurotrophic  factor in rat salivary glands.
abstract: Plasma brain-derived neurotrophic factor (BDNF) levels are associated with several neural disorders. Previously, we reported that BDNF is produced from salivary glands under acute immobilization stress. Additionally, salivary glands are the origin of plasma BDNF during stress; however, the association between the expression of BDNF by the salivary glands under chronic stress conditions is not known. In the present study, we investigated whether plasma BDNF levels in chronic stress depend on the salivary glands. Expression of BDNF mRNA and protein were identified in the submandibular glands when male rats were exposed to chronic restraint stress (12 h daily for 22 days). Chronic stress significantly increased plasma BDNF concentration, as well as adrenocorticotropic hormone and corticosterone levels, but was not altered under chronic stress in bilaterally sialoadenectomized rats. Since chronic stress increases plasma BDNF levels in the sialoadenectomized rat model, the plasma BDNF level was not dependent on BDNF from the salivary glands. Although the salivary glands were the source of plasma BDNF in acute stress conditions in our previous study, it seems that that the increased BDNF expression in the salivary glands in chronic stress does not contribute importantly to the increased circulating BDNF level. The increased plasma BDNF levels may play important roles in homeostasis under stress conditions.
authors: Department of Craniofacial Growth and Development Dentistry, Division of Orthodontics, Kanagawa Dental College, Yokosuka, Kanagawa, Japan.
authors: Juri
authors: J
authors: Saruta
authors: Taeki
authors: T
authors: Lee
authors: Masayoshi
authors: M
authors: Shirasu
authors: Takeshi
authors: T
authors: Takahashi
authors: Chikatoshi
authors: C
authors: Sato
authors: Sadao
authors: S
authors: Sato
authors: Keiichi
authors: K
authors: Tsukinoki
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D003345:Corticosterone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D010913:Pituitary-Adrenal System / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000502:physiology*; D013363:Submandibular Gland / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone