title: Effects of moderate versus deep hypothermic circulatory arrest and selective cerebral perfusion on cerebrospinal fluid proteomic profiles in a piglet model of cardiopulmonary bypass.
abstract: OBJECTIVE
  Our objective was to compare protein profiles of cerebrospinal fluid between control animals and those subjected to cardiopulmonary bypass after moderate versus deep hypothermic circulatory arrest with selective cerebral perfusion.
  
  
  METHODS
  Immature Yorkshire piglets were assigned to one of four study groups: (1) deep hypothermic circulatory arrest at 18 degrees C, (2) deep hypothermic circulatory arrest at 18 degrees C with selective cerebral perfusion, (3) moderate hypothermic circulatory arrest at 25 degrees C with selective cerebral perfusion, or (4) age-matched control animals without surgery. Animals undergoing cardiopulmonary bypass were cooled to their assigned group temperature and exposed to 1 hour of hypothermic circulatory arrest. After arrest, animals were rewarmed, weaned off bypass, and allowed to recover for 4 hours. Cerebrospinal fluid collected from surgical animals after the recovery period was compared with cerebrospinal fluid from controls by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Protein spectra were analyzed for differences between groups by Mann-Whitney U test and false discovery rate analysis.
  
  
  RESULTS
  Baseline and postbypass physiologic parameters were similar in all surgical groups. A total of 194 protein peaks were detected. Compared with controls, groups 1, 2, and 3 had 64, 100, and 13 peaks that were significantly different, respectively (P < .05). Three of these peaks were present in all three groups. Cerebrospinal fluid protein profiles in animals undergoing cardiopulmonary bypass with moderate hypothermic circulatory arrest (group 3) were more similar to controls than either of the groups subjected to deep hypothermia.
  
  
  CONCLUSIONS
  The mass spectra of cerebrospinal fluid proteins are altered in piglets exposed to cardiopulmonary bypass and hypothermic circulatory arrest. Moderate hypothermic circulatory arrest (25 degrees C) with selective cerebral perfusion compared with deep hypothermic circulatory arrest (18 degrees C) is associated with fewer changes in cerebrospinal fluid proteins, when compared with nonbypass controls.
authors: Department of Pediatrics, Wilford Hall USAF Medical Center, Lackland Air Force Base, San Antonio, TX 78236-5300, USA. taslim@gmail.com
authors: Taslim
authors: T
authors: Allibhai
authors: Robert
authors: R
authors: DiGeronimo
authors: John
authors: J
authors: Whitin
authors: Jorge
authors: J
authors: Salazar
authors: Tom To-Sang
authors: TT
authors: Yu
authors: Xuefeng Bruce
authors: XB
authors: Ling
authors: Harvey
authors: H
authors: Cohen
authors: Patricia
authors: P
authors: Dixon
authors: Ashima
authors: A
authors: Madan
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007036:Hypothermia, Induced / Q000379:methods*; D013058:Mass Spectrometry; D010477:Perfusion; D012039:Regional Blood Flow; D013552:Swine
chemical_list: D002556:Cerebrospinal Fluid Proteins