Association of Fas/Apo1 gene promoter (-670 A/G) polymorphism in Tunisian patients with IBD.
OBJECTIVE
To detect a possible association between the polymorphism of the (-670 A/G) Fas/Apo1 gene promoter and susceptibility to Crohn's disease (CD) and ulcerative colitis (UC) in the Tunisian population.


METHODS
The (-670 A/G) Fas polymorphism was analyzed in 105 patients with CD, 59 patients with UC, and 100 controls using the polymerase chain reaction restriction fragment length polymorphism method.


RESULTS
Significantly lower frequencies of the Fas -670 A allele and A/A homozygous individuals were observed in CD and UC patients when compared with controls. Analysis of (-670 A/G) Fas polymorphism with respect to sex in CD and UC showed a significant difference in A/A genotypes between female patients and controls (P corrected = 0.004 in CD patients and P corrected = 0.02 in UC patients, respectively). Analysis also showed a statistically significant association between genotype AA of the (-670 A/G) polymorphism and the ileum localization of the lesions (P corrected = 0.048) and between genotype GG and the colon localization (P corrected = 0.009). The analysis of inflammatory bowel disease patients according to clinical behavior revealed no difference.


CONCLUSIONS
Fas-670 polymorphism was associated with the development of CD and UC in the Tunisian population.
Ben Aleya
Walid
W
Laboratory of Immunology, EPS Charles Nicolle, Bd 9 Avril, 1006 Bab Saadoun, Tunis, Tunisia. b_a_w@hotmail.fr
Sfar
Imen
I
Mouelhi
Leila
L
Aouadi
Houda
H
Makhlouf
Mouna
M
Ayed-Jendoubi
Salwa
S
Matri
Samira
S
Filali
Azza
A
Najjar
Taoufik
T
Ben Abdallah
Taeib
T
Ayed
Khaled
K
Gorgi
Yousr
Y
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics*; D003424:Crohn Disease / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014416:Tunisia; D055815:Young Adult; D019014:fas Receptor / Q000235:genetics*
D019014:fas Receptor