Methanobacterium veterum sp. nov., from ancient Siberian permafrost.
A methanogenic archaeon, strain MK4(T), was isolated from ancient permafrost after long-term selective anaerobic cultivation. The cells were rods, 2.0-8.0 microm long and 0.40-0.45 microm wide, and stained Gram-negative. Optimal growth was observed at 28 degrees C and pH 7.0-7.2 and in 0.05 M NaCl. The isolate used H(2) plus CO(2), methylamine plus H(2) and methanol plus H(2) as sources for growth and methanogenesis. Phylogenetic analysis of the 16S rRNA gene sequence of the strain showed close affinity with Methanobacterium bryantii (similarity >99 % to the type strain). On the basis of the level of DNA-DNA hybridization (62 %) between strain MK4(T) and Methanobacterium bryantii VKM B-1629(T) and phenotypic and phylogenetic differences, strain MK4(T) was assigned to a novel species of the genus Methanobacterium, Methanobacterium veterum sp. nov., with the type strain MK4(T) (=DSM 19849(T) =VKM B-2440(T)).
Krivushin
Kirill V
KV
Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russian Federation.
Shcherbakova
Viktoria A
VA
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russian Federation.
Petrovskaya
Lada E
LE
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya, 16/10, 117997 GSP, Moscow V-437, Russian Federation.
Rivkina
Elizaveta M
EM
Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russian Federation.
D001483:Base Sequence; D005615:Freezing; D008697:Methane / Q000378:metabolism; D017013:Methanobacteriaceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012800:Siberia; D012988:Soil Microbiology
D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D008697:Methane