Conformational stabilities of guinea pig OCP1 and OCP2.
OCP1 and OCP2, the most abundant proteins in the cochlea, are evidently subunits of an SCF E3 ubiquitin ligase. Although transcribed from a distinct gene, OCP2 is identical to Skp1. OCP1 is equivalent to the F-box protein known as Fbs1, Fbx2, or NFB42 - previously shown to bind N-glycosylated proteins and believed to function in the retrieval and recycling of misfolded proteins. The high concentrations of OCP1 and OCP2 in the cochlea suggest that the OCP1-OCP2 heterodimer may serve an additional function independent of its role in a canonical SCF complex. At 25 degrees C, urea-induced denaturation of OCP1 is slow, but reversible. The data suggest that the protein possesses one or more disordered regions, a conclusion supported by analysis of the far-UV circular dichroism spectrum and the appearance of the (1)H, (15)N-HSQC spectrum. Thermal denaturation of OCP1 is irreversible, evidently due to formation of high molecular weight aggregates. Analysis with a kinetic model yields an estimate for the activation energy for unfolding of 49 kcal/mol. Urea denaturation data for OCP2 returns DeltaG(o) and m values of 6.2 kcal/mol and 1.5 kcal mol(-)(1) M(-1), respectively. In contrast to OCP1, thermal denaturation of OCP2 is reversible. In phosphate-buffered saline, at pH 7.40, the protein exhibits a DeltaH(vH)/DeltaH(cal) ratio of 1.69, suggesting that denaturation proceeds largely from the native dimer directly to the unfolded state. OCP1 and OCP2 associate tightly at room temperature. However, DSC data for the complex suggest that they denature independently, consistent with the highly exothermic enthalpy of complex formation reported previously.
Tan
Anmin
A
Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States.
Henzl
Michael T
MT
D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D002152:Calorimetry, Differential Scanning; D044783:F-Box Proteins / Q000737:chemistry*; D006168:Guinea Pigs; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009925:Organ of Corti / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D055503:Protein Multimerization; D055550:Protein Stability; D017433:Protein Structure, Secondary; D044786:S-Phase Kinase-Associated Proteins / Q000737:chemistry*; D044843:SKP Cullin F-Box Protein Ligases / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry
D044783:F-Box Proteins; D044786:S-Phase Kinase-Associated Proteins; D014508:Urea; D044843:SKP Cullin F-Box Protein Ligases