Amycolatopsis halophila sp. nov., a halophilic actinomycete isolated from a salt lake.
A novel halophilic actinomycete, designated strain YIM 93223(T), was isolated from a salt lake in Xinjiang Province, north-west China, and was subjected to a polyphasic taxonomic study. The isolate grew at 25-45 degrees C, at pH 6-8 and in the presence of 1-15 % (w/v) NaCl; no growth was observed in the absence of NaCl. Strain YIM 93223(T) contained meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids, and glucose and galactose as major whole-cell-wall sugars. Major fatty acids were iso-C(16 : 0), C(16 : 0) and C(16 : 1)omega7c/iso-C(15 : 0) 2-OH. MK-8(H(4)) was the predominant menaquinone. The genomic DNA G+C content was 66.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 93223(T) belongs to the genus Amycolatopsis. It shared highest 16S rRNA gene sequence similarity with Amycolatopsis palatopharyngis 1BDZ(T) (96.6 %) and Amycolatopsis marina Ms392A(T) (96.4 %), but lower values (94.5-96.2 %) with the type strains of other recognized species of the genus Amycolatopsis. On the basis of the data from this polyphasic study, strain YIM 93223(T) is considered to represent a novel species of the genus Amycolatopsis, for which the name Amycolatopsis halophila sp. nov. is proposed. The type strain is YIM 93223(T) (=DSM 45216(T) =KCTC 19403(T)).
Tang
Shu-Kun
SK
The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Wang
Yun
Y
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang 830091, PR China.
Guan
Tong-Wei
TW
Key Laboratary of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar, Xinjiang 843300, PR China.
Lee
Jae-Chan
JC
Functional Metabolite Research Center KRIBB, 52 Eoeun-dong, Yuseong gu, Daejeon 305-806, Republic of Korea.
Kim
Chang-Jin
CJ
Functional Metabolite Research Center KRIBB, 52 Eoeun-dong, Yuseong gu, Daejeon 305-806, Republic of Korea.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride