Induction of insulin resistance by phosphatidylinositol-3-kinase inhibitor in porcine granulosa cells.
Insulin resistance on porcine granulosa cells was induced by wortmannin, the phosphatidylinositol-3-kinase inhibitor, and insulin signaling key molecules were investigated including glut(4) and mitogen-activated protein kinase. Granulosa insulin resistance decreased glut(4) expression but increased mitogen-activated protein kinase, indicating the cross talk between the metabolic and mitogenic pathways of insulin signaling in the ovary.
Yan
Miaoe
M
Department of Obstetrics and Gynecology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, People's Republic of China.
Wang
Jing
J
Wu
Xiaoke
X
Hou
Lihui
L
Kuang
Haixue
H
Wang
Yongyan
Y
D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D013552:Swine; D000077191:Wortmannin
D000730:Androstadienes; D051275:Glucose Transporter Type 4; D010726:Phosphodiesterase Inhibitors; D000081082:Phosphoinositide-3 Kinase Inhibitors; D000077191:Wortmannin