title: The complete genomic sequence of an in vivo low replicating BLV strain.
abstract: DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.
authors: Department of Medicine, Upstate Medical University, Syracuse, New York 13210, USA. dubes@upstate.edu
authors: Syamalima
authors: S
authors: Dube
authors: Lynn
authors: L
authors: Abbott
authors: Dipak K
authors: DK
authors: Dube
authors: Guillermina
authors: G
authors: Dolcini
authors: Silvina
authors: S
authors: Gutierrez
authors: Carolina
authors: C
authors: Ceriani
authors: Marcela
authors: M
authors: Juliarena
authors: Jorge
authors: J
authors: Ferrer
authors: Raisa
authors: R
authors: Perzova
authors: Bernard J
authors: BJ
authors: Poiesz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina; D001483:Base Sequence; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016583:Enzootic Bovine Leukosis / Q000821:virology; D016679:Genome, Viral; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000502:physiology; D008214:Lymphocytes / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014779:Virus Replication
chemical_list: D004279:DNA, Viral