MicroRNA expression profile in non-cancerous colonic tissue associated with lymph node metastasis of colon cancer.
OBJECTIVE
To identify microRNA expression patterns associated with the lymph node metastasis of colon cancer.


METHODS
MicroRNA were isolated from six frozen non-cancerous surrounding colonic tissues derived from stage II-III colon cancer patients with (n = 3) and without (n = 3) lymph node metastasis. We compared the microRNA expression profiles of the six non-cancerous colonic tissues from two colon cancer patient groups; those with confirmed lymph node metastasis, termed the lymph node positive group, and those without detectable lymph node metastasis, termed the lymph node negative group. MicroRNA expression was analyzed with Agilent microarrays containing 723 human microRNA probes. We validated the expression level of differentially expressed microRNA using quantitative real-time PCR analysis.


RESULTS
Two microRNA (hsa-miR-129*, hsa-miR-137) were differentially expressed in the lymph node positive group compared with the lymph node negative group. The expression level of hsa-miR-137 was quantified via quantitative real-time PCR analysis for validation. Hsa-miR-137 expression was significantly upregulated nearly 6.6-fold in lymph node positive specimens (P = 0.036). The quantitative real-time PCR result correlates with the microarray finding.


CONCLUSIONS
The non-cancerous colonic tissues from colon cancer patients with lymph node metastasis have a significantly different microRNA expression profile compared to that from colon cancer patients without lymph node metastasis. The differentially expressed microRNA could have relevance to the lymph node metastasis of colon cancer and may provide a simple profiling method to assist in identifying patients with lymph node metastasis. Besides, these data might offer new ideas for preventing and controlling lymphatic metastasis in colon cancer.
Huang
Zhi Ming
ZM
Department of Gastroenterology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang Province, China.
Yang
Ji
J
Shen
Xiao Ying
XY
Zhang
Xiao Yan
XY
Meng
Fan Sheng
FS
Xu
Jiang Tao
JT
Zhang
Bao Feng
BF
Gao
Heng Jun
HJ
D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
C545567:MIRN137 microRNA, human; D035683:MicroRNAs; C541915:Mirn129 microRNA, human