title: Pathological and parasitological protection in goats immunised with recombinant cathepsin L1 and challenged with Fasciola hepatica.
abstract: Fluke burdens, faecal egg output and hepatic damage were assessed in goats immunised with recombinant cathepsin L1 (rCL1) plus Quil A (n=7) or Quil A alone (control; n=7) and challenged with Fasciola hepatica. There were no significant differences in fluke burdens (56+/-26 vs. 92+/-53), average fluke length (20.9+/-3.0 vs. 21.0+/-3.4mm) or faecal egg output (525+/-533 vs. 758+/-677.8 eggs per gram at 17 weeks post-infection) between vaccinated and infected control groups, respectively, but high individual variability was evident. Morphometric and histopathological studies showed reduced hepatic damage in the vaccinated group compared to the infected control, but high individual variability was also recorded. Further vaccine trials in goats should be carried out using a larger number of animals to evaluate rCL1 as a vaccine for fasciolosis in goats.
authors: Department of Anatomy and Comparative Pathology, School of Veterinary Medicine, University of Córdoba, Edificio de Sanidad Animal, Campus de Rabanales, Córdoba, Spain.
authors: Rafael A
authors: RA
authors: Pérez-Ecija
authors: Ricardo E
authors: RE
authors: Mendes
authors: Rafael
authors: R
authors: Zafra
authors: Leandro
authors: L
authors: Buffonni
authors: Alvaro
authors: A
authors: Martínez-Moreno
authors: José
authors: J
authors: Pérez
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D002403:Cathepsins / Q000276:immunology*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D015511:Goat Diseases / Q000276:immunology / Q000469:parasitology / Q000473:pathology / Q000517:prevention & control*; D006041:Goats; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D008099:Liver / Q000469:parasitology / Q000473:pathology; D010270:Parasite Egg Count / Q000662:veterinary; D062605:Quillaja Saponins; D011994:Recombinant Proteins / Q000276:immunology; D012503:Saponins / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D062605:Quillaja Saponins; D011994:Recombinant Proteins; D012503:Saponins; C046386:Quil A; D002403:Cathepsins; C509464:cathepsin L1, Fasciola hepatica