The use of differential receptors to pattern peptide phosphorylation.
An array sensing scheme for the differentiation of small peptides and their phosphorylated analogues is introduced. The technique involves a series of receptors created by appending random peptides to a C(3v) symmetric scaffold that binds phosphomonoesters. Five specific peptide sequences were selected through a screening technique. In addition to cross reactivity being created by the peptides in the receptors, three metal ions and three pH indicators are used to create a suite of 45 indicator displacement assays. The colorimetric data from the 45 sensing ensembles is collected in a 96-well plate reader, and linear discriminant analysis gives patterns resulting in 100% classification of the peptides. The approach demonstrates a generalizable principle to create pattern-based recognition protocols for complex analytes.
Zhang
Tianzhi
T
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA.
Edwards
Nicola Y
NY
Bonizzoni
Marco
M
Anslyn
Eric V
EV
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques; D015195:Drug Design; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D013379:Substrate Specificity; D051844:alpha-Synuclein / Q000737:chemistry / Q000378:metabolism*
D008670:Metals; D009842:Oligopeptides; D051844:alpha-Synuclein