Flavobacterium fluvii sp. nov., isolated from stream sediment.
An aerobic, yellow-pigmented, Gram-staining-negative bacterium, designated strain H7(T), was isolated from sediment of Gazwa stream in Jinju city, South Korea. Cells of strain H7(T) were non-motile, straight rods that produced flexirubin pigments and showed catalase- and oxidase activities. Strain H7(T) contained iso-C(15 : 0,) anteiso-C(15 : 0) and summed feature 3 (comprising C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH) as the main fatty acids and menaquinone-6 (MK-6) as the major isoprenoid quinone. The DNA G+C content of strain H7(T) was 37.2 mol%. Comparative 16S rRNA gene sequence analysis showed that strain H7(T) formed a distinct phyletic line within the genus Flavobacterium. Based on 16S rRNA gene sequence similarities, the novel strain was most closely related to Flavobacterium limicola ST-82(T) and Flavobacterium resistens BD-b365(T) with 97.2 % and 97.0 % gene sequence similarities, respectively. The DNA-DNA relatedness of the novel strain with these species was only 18 % and 14 %, respectively. On the basis of phenotypic data and molecular properties, strain H7(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium fluvii sp. nov. is proposed. The type strain is H7(T) (=KACC 12818(T)=DSM 19978(T)).
Lee
Se Hee
SH
Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Kim
Jeong Myeong
JM
Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Lee
Jung Ro
JR
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Park
Woojun
W
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-701, Republic of Korea.
Jeon
Che Ok
CO
Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.
D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification* / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D045483:Rivers / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S