title: Millimeter-wave exposure promotes the differentiation of bone marrow stromal cells into cells with a neural phenotype.
abstract: This study investigated the ability of millimeter-wave (MMW) to promote the differentiation of bone marrow stromal cells (BMSCs) into cells with a neural phenotype. The BMSCs were primarily cultured. At passage 3, the cells were induced by beta-mercaptoethanol (BME) in combination with MMW or BME alone. The expressions of nucleostemin (NS) and neuron-specific enolase (NSE) were detected by immunofluorescent staining and Western blotting respectively to identify the differentiation. The untreated BMSCs predominately expressed NS. After induced by BME and MMW, the BMSCs exhibited a dramatic decrease in NS expression and increase in NSE expression. The differentiation rate of the cells treated with BME and MMW in combination was significantly higher than that of the cells treated with BME alone (P<0.05). It was concluded that MMW exposure enhanced the inducing effect of BME on the differentiation of BMSCs into cells with a neural phenotype.
authors: Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. tongyq01@yahoo.cn
authors: Yeqing
authors: Y
authors: Tong
authors: Zhaohui
authors: Z
authors: Yang
authors: Di
authors: D
authors: Yang
authors: Huikuan
authors: H
authors: Chu
authors: Min
authors: M
authors: Qu
authors: Guanlan
authors: G
authors: Liu
authors: Yan
authors: Y
authors: Wu
authors: Shenghong
authors: S
authors: Liu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects*; D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects*; D002478:Cells, Cultured; D008623:Mercaptoethanol / Q000494:pharmacology; D008872:Microwaves; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D051381:Rats; D017154:Stromal Cells / Q000166:cytology / Q000528:radiation effects*
chemical_list: D008623:Mercaptoethanol