Serratia glossinae sp. nov., isolated from the midgut of the tsetse fly Glossina palpalis gambiensis.
We report the isolation of a novel bacterium, strain C1(T), from the midgut of the tsetse fly Glossina palpalis gambiensis, one of the vector insects responsible for transmission of the trypanosomes that cause sleeping sickness in sub-Saharan African countries. Strain C1(T) is a motile, facultatively anaerobic, rod-like bacterium (0.8-1.0 microm in diameter; 2-6 microm long) that grows as single cells or in chains. Optimum growth occurred at 25-35 degrees C, at pH 6.7-8.4 and in medium containing 5-20 g NaCl l(-1). The bacterium hydrolysed urea and used L-lysine, L-ornithine, citrate, pyruvate, D-glucose, D-mannitol, inositol, D-sorbitol, melibiose, amygdalin, L-arabinose, arbutin, aesculin, D-fructose, D-galactose, glycerol, maltose, D-mannose, raffinose, trehalose and d-xylose; it produced acetoin, reduced nitrate to nitrite and was positive for beta-galactosidase and catalase. The DNA G+C content was 53.6 mol%. It was related phylogenetically to members of the genus Serratia, family Enterobacteriaceae, the type strain of Serratia fonticola being its closest relative (99 % similarity between 16S rRNA gene sequences). However, DNA-DNA relatedness between strain C1(T) and S. fonticola DSM 4576(T) was only 37.15 %. Therefore, on the basis of morphological, nutritional, physiological and fatty acid analysis and genetic criteria, strain C1(T) is proposed to be assigned to a novel Serratia species, Serratia glossinae sp. nov. (type strain C1(T) =DSM 22080(T) =CCUG 57457(T)).
Geiger
A
A
UMR 177, IRD-CIRAD, CIRAD TA A-17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
Fardeau
M-L
ML
Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Falsen
E
E
CCUG, Culture Collection, University of Göteborg, Guldhedsgatan 10, SE-413 46 Göteborg, Sweden.
Ollivier
B
B
Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Cuny
G
G
UMR 177, IRD-CIRAD, CIRAD TA A-17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001482:Base Composition / Q000235:genetics; D002050:Burkina Faso; D002374:Catalase / Q000378:metabolism; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011679:Pupa / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012705:Serratia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014353:Trypanosomiasis, African / Q000635:transmission; D014370:Tsetse Flies / Q000382:microbiology* / Q000472:pathogenicity; D014508:Urea / Q000378:metabolism
D017931:DNA Primers; D004269:DNA, Bacterial; D009566:Nitrates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014508:Urea; D002374:Catalase