Biochemical, behavioural and quantitative alterations in cerebellum of Swiss albino mice following irradiation and its modulation by Grewia asiatica.
OBJECTIVE
To study the biochemical, quantitative histopathological and behavioural changes after 5 Gy whole body irradiation and its modulation by supplementation of Grewia asiatica extract (GAE) on male Swiss albino mice up to 30 days.


METHODS
For this, healthy mice from an inbred colony were divided into five groups: (i) Control (vehicle treated); (ii) GAE treated--mice in this group were orally supplemented with GAE (700 mg/kg body weight (bw)/day) once daily for 15 consecutive days; (iii) Irradiated mice; (iv) GAE + irradiated--mice in this group were orally supplemented GAE for 15 days (once a day) prior to irradiation; and (v) Irradiated + GAE--mice in this group were administered GAE orally for 15 days (once a day) consequently after irradiation.


RESULTS
Marked radiation induced changes in the amount of cerebellar lipid peroxidation (LPO), Glutathione (GSH), protein, nucleic acids and histopathological changes could be significantly (p < 0.001) ameliorated specially at later intervals by supplementation of GAE prior to and post irradiation. Radiation induced deficits in learning and memory were also significantly (p < 0.001) ameliorated.


CONCLUSIONS
The result of present study showed that prior/post-supplementation of G. asiatica has radioprotective potential as well as neuroprotective properties against the radiation.
Sisodia
Rashmi
R
Radiation Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, India. rashsisodia@yahoo.co.in
Singh
Smita
S
D000284:Administration, Oral; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology / Q000528:radiation effects*; D005978:Glutathione / Q000378:metabolism; D032382:Grewia / Q000737:chemistry*; D007858:Learning / Q000187:drug effects / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D010936:Plant Extracts; D011837:Radiation-Protective Agents; D005978:Glutathione