title: Dual-isotope single-photon emission computed tomography for dopamine and serotonin transporters in normal and Parkinsonian monkey brains.
abstract: BACKGROUND
  Parkinson's disease (PD) affects both dopaminergic and serotonergic systems. In this study, we simultaneously evaluated dopamine and serotonin transporters in primates using dual-isotope single-photon emission computed tomography (SPECT) imaging and compared the results with traditional single-isotope imaging.
  
  
  METHODS
  Four healthy and one 6-OHDA-induced PD monkeys were used for this study. SPECT was performed over 4 h after individual or simultaneous injection of [(99m)Tc]TRODAT-1 (a dopamine transporter imaging agent) and [(123)I]ADAM (a serotonin transporter imaging agent).
  
  
  RESULTS
  The results showed that the image quality and uptake ratios in different brain regions were comparable between single- and dual-isotope studies. The striatal [(99m)Tc]TRODAT-1 uptake in the PD monkey was markedly lower than that in normal monkeys. The uptake of [(123)I]ADAM in the midbrain of the PD monkey was comparable to that in the normal monkeys, but there were decreased uptakes in the thalamus and striatum of the PD monkey.
  
  
  CONCLUSIONS
  Our results suggest that dual-isotope SPECT using [(99m)Tc]TRODAT-1 and [(123)I]ADAM can simultaneously evaluate changes in dopaminergic and serotonergic systems in a PD model.
authors: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan.
authors: I-Hsun
authors: IH
authors: Li
authors: Wen-Sheng
authors: WS
authors: Huang
authors: Chin-Bin
authors: CB
authors: Yeh
authors: Mei-Hsiu
authors: MH
authors: Liao
authors: Chia-Chieh
authors: CC
authors: Chen
authors: Lie-Hang
authors: LH
authors: Shen
authors: Jiang-Chuan
authors: JC
authors: Liu
authors: Kuo-Hsing
authors: KH
authors: Ma
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism; D002928:Cinanserin / Q000031:analogs & derivatives* / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism*; D005240:Feasibility Studies; D008251:Macaca; D015609:Organotechnetium Compounds / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology; D010300:Parkinson Disease / Q000000981:diagnostic imaging* / Q000209:etiology / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D013997:Time Factors; D015899:Tomography, Emission-Computed, Single-Photon; D014326:Tropanes / Q000378:metabolism
chemical_list: C413491:2-((2-((dimethylamino)methyl)phenyl)thio)-5-iodophenylamine; D050483:Dopamine Plasma Membrane Transport Proteins; D015609:Organotechnetium Compounds; D050486:Serotonin Plasma Membrane Transport Proteins; D014326:Tropanes; C104196:technetium Tc 99m TRODAT-1; D016627:Oxidopamine; D002928:Cinanserin