Sphingobium chinhatense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH dumpsite.
A yellow-pigmented, hexachlorocyclohexane (HCH)-degrading bacterium, strain IP26(T), was isolated from an HCH dumpsite and subjected to a polyphasic analysis in order to determine its taxonomic position. Strain IP26(T) showed maximum 16S rRNA gene sequence similarity with Sphingobium francense Sp+(T) (98.5 %), Sphingobium japonicum UT26(T) (98.4 %) and Sphingobium indicum B90A(T) (98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences also showed that strain IP26(T) formed a cluster with these three HCH-degrading strains. Chemotaxonomic data (major polyamine, spermidine; major quinone, ubiquinone with ten isoprene units; major polar lipids, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphotidylcholine; and presence of 2-hydroxy fatty acid) supported inclusion of strain IP26(T) in the genus Sphingobium. However, the results of DNA-DNA hybridization and morphological and biochemical tests clearly allowed phenotypic and genotypic differentiation of strain IP26(T) from recognized species of the genus Sphingobium. Strain IP26(T) thus represents a novel species of the genus Sphingobium for which the name Sphingobium chinhatense sp. nov. is proposed. The type strain is IP26(T) (=MTCC8598(T) =CCM 7432(T)).
Dadhwal
Mandeep
M
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
Jit
Simran
S
Kumari
Hansi
H
Lal
Rup
R
D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D007220:Industrial Waste; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D001556:Hexachlorocyclohexane