title: Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas.
abstract: Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18-20 % (w/v) NaCl, pH 6.0-8.5 and 37-40 degrees C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA-DNA hybridization values between Nmn. pharaonis DSM 2160(T) and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11(T) (=JCM 14361(T) =CECT 7526(T) =DSM 18674(T)). An emended description of the genus Natronomonas is also provided.
authors: Microbiological Diagnostic Unit, University of Melbourne, Parkville 3052, Australia.
authors: David G
authors: DG
authors: Burns
authors: Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North 4442, New Zealand.
authors: Peter H
authors: PH
authors: Janssen
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan.
authors: Takashi
authors: T
authors: Itoh
authors: Bio-Nano Electronics Research Centre, Toyo University, Kawagoe 350-8585, Japan.
authors: Hiroaki
authors: H
authors: Minegishi
authors: Bio-Nano Electronics Research Centre, Toyo University, Kawagoe 350-8585, Japan.
authors: Ron
authors: R
authors: Usami
authors: Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba 278-0043, Japan.
authors: Masahiro
authors: M
authors: Kamekura
authors: Max Planck Institute of Biochemistry, Department of Membrane Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
authors: Mike L
authors: ML
authors: Dyall-Smith
mesh_terms: D003460:Crystallization; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride