title: Filter-exchange PGSE NMR determination of cell membrane permeability.
abstract: A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.
authors: Physical Chemistry, Lund University, Lund, Sweden. ingrid.aslund@fkem1.lu.se
authors: Ingrid
authors: I
authors: Aslund
authors: Agnieszka
authors: A
authors: Nowacka
authors: Markus
authors: M
authors: Nilsson
authors: Daniel
authors: D
authors: Topgaard
mesh_terms: D000465:Algorithms; D002462:Cell Membrane / Q000737:chemistry*; D002463:Cell Membrane Permeability; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water