Half-life mapping of nitroxyl radicals with three-dimensional electron paramagnetic resonance imaging at an interval of 3.6 seconds.
This technical note reports a continuous-wave electron paramagnetic resonance (CW-EPR) imager that can visualize the distribution of free radicals with a half-life of subminutes in three-dimensional (3D) space. A total of 46 EPR spectra under magnetic field gradients, called projections, were obtained for image reconstruction at an interval of 3.6 s. A shortened data-acquisition time was achieved with the use of analog signals that drove field gradient coils in the imager. 3D mapping of the half-lives of nitroxyl radicals (4-hydroxyl-2,2,6,6-tetramethyl-piperidinyl-1-oxyl) was demonstrated in their reduction reaction with ascorbic acid. Inhomogeneous half-lives were clearly mapped pixel-by-pixel in a sample tube.
Sato-Akaba
Hideo
H
Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan.
Kuwahara
Yoko
Y
Fujii
Hirotada
H
Hirata
Hiroshi
H
D001205:Ascorbic Acid / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006207:Half-Life; D021621:Imaging, Three-Dimensional / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D009589:Nitrogen Oxides / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013997:Time Factors
D009589:Nitrogen Oxides; C039900:nitroxyl; D001205:Ascorbic Acid