title: Two novel SNPs in coding region of the caprine Fat-inducing transcript gene and their association with growth traits.
abstract: Fat-inducing transcript (FIT) are endoplasmic reticulum resident membrane proteins that induce lipid droplet accumulation. It plays a crucial role in the fundamental process of fat storing. In this study, on the basis of PCR-SSCP and DNA sequencing methods, polymorphisms of the FIT gene were detected in 254 individuals from three goat breeds. The results showed that only P(5) locus had two SNPs, resulting in a synonymous mutation (NM_001103095: m.422T > A/G resulting in S124S) and a missense mutation (NM_001103095: m.454 A > G resulting in Q135R). The associations between polymorphic loci and the growth traits of Boer and Xuhuai goat were analysed, and significant association were found in body height and chest circumference index (P < 0.05). Genotype BB of the Boer was associated with a higher chest circumference index (P < 0.05) than Genotype AC. In Xuhuai breed, Genotype BB was associated with a lower body height (P < 0.05) than Genotype AA. However, Genotype BB and AC had greater chest circumference index than Genotype AA (P < 0.01).
authors: Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, Jiangsu, People's Republic of China.
authors: Jiajie
authors: J
authors: Sun
authors: Chunlei
authors: C
authors: Zhang
authors: Hong
authors: H
authors: Chen
authors: Xingtang
authors: X
authors: Fang
authors: Qijiang
authors: Q
authors: Jin
authors: Danxia
authors: D
authors: Chen
authors: Xiuying
authors: X
authors: Shi
authors: Yu
authors: Y
authors: Du
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002681:China; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D006041:Goats / Q000235:genetics* / Q000254:growth & development*; D006239:Haplotypes / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D008565:Membrane Proteins