Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms.
Analysis of receptor-ligand interactions in vivo is key to biology but poses a considerable challenge to quantitative microscopy. Here we combine static-volume, two-focus and dual-color scanning fluorescence correlation spectroscopy to solve this task at cellular resolution in complex biological environments. We quantified the mobility of fibroblast growth factor receptors Fgfr1 and Fgfr4 in cell membranes of living zebrafish embryos and determined their in vivo binding affinities to their ligand Fgf8.
Ries
Jonas
J
Biotechnology Center, Technical University of Dresden, Dresden, Germany.
Yu
Shuizi Rachel
SR
Burkhardt
Markus
M
Brand
Michael
M
Schwille
Petra
P
D000818:Animals; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D011485:Protein Binding; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism*; D051499:Receptor, Fibroblast Growth Factor, Type 4 / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D015027:Zebrafish / Q000196:embryology; D029961:Zebrafish Proteins / Q000378:metabolism*
D017468:Receptors, Fibroblast Growth Factor; D029961:Zebrafish Proteins; C517774:fgf8a protein, zebrafish; D005346:Fibroblast Growth Factors; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051499:Receptor, Fibroblast Growth Factor, Type 4; C496375:fgfr4 protein, zebrafish