title: Cotransplantation with xenogenetic neonatal porcine sertoli cells significantly prolongs islet allograft survival in nonimmunosuppressive rats.
abstract: BACKGROUND
  In addition to possessing immune privileged properties, Sertoli cells are known to actively suppress responses to cotransplanted cells. An important question is whether this "bystander suppression" is limited to cells of the same origin as the Sertoli cells or whether suppression extends to unrelated cells.
  
  
  METHODS
  Neonatal porcine Sertoli cells (NPSCs) were transplanted with allogeneic islets (Sprague-Dawley rat) into immune competent Wistar rats subsequent to induction of diabetes by alloxan administration.
  
  
  RESULTS
  Although allogeneic islets alone had a mean survival time of 5.67+/-0.94 days, islets cotransplanted with 1.5 x 10 xenogeneic NPSCs displayed a survival of 8.33+/-0.58 days. Increasing the concentration of NPSCs to 1.0 x10 yielded a further increase in survival to 16.33+/-1.53 days. Augmented islet survival was associated with reduced lymphocytic infiltrate and elevated numbers of Sox9 positive cells. Mechanistically, it seemed that Fas ligand was not involved in prolongation of survival because in contrast to adult Sertoli cells, NPSCs lacked expression of this gene.
  
  
  CONCLUSIONS
  These data suggest that xenogeneic Sertoli cells exert a global immune suppressive effect that extends across species barriers in a stringent model of alloimmune rejection. The combination of NPSCs with other immune modulatory regimes may yield novel approaches toward prevention of allo-islet transplant rejection.
authors: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Zhuzeng
authors: Z
authors: Yin
authors: Dong
authors: D
authors: Chen
authors: Feng
authors: F
authors: Hu
authors: Yongle
authors: Y
authors: Ruan
authors: Junhua
authors: J
authors: Li
authors: Lu
authors: L
authors: Wang
authors: Ying
authors: Y
authors: Xiang
authors: Lin
authors: L
authors: Xie
authors: Ximo
authors: X
authors: Wang
authors: Thomas E
authors: TE
authors: Ichim
authors: Shi
authors: S
authors: Chen
authors: Gang
authors: G
authors: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D024201:Bystander Effect; D002470:Cell Survival; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000378:metabolism / Q000601:surgery*; D053222:Fas Ligand Protein / Q000378:metabolism; D005260:Female; D006085:Graft Survival; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007165:Immunosuppression Therapy / Q000379:methods*; D007328:Insulin / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D055758:SOX9 Transcription Factor / Q000378:metabolism; D012708:Sertoli Cells / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D013552:Swine; D013997:Time Factors; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous
chemical_list: D053222:Fas Ligand Protein; D007328:Insulin; D019253:Proto-Oncogene Proteins c-bcl-2; D055758:SOX9 Transcription Factor; D051547:Heme Oxygenase-1