title: Comparative analysis of uncoupling protein 4 distribution in various tissues under physiological conditions and during development.
abstract: UCP4 is a member of the mitochondrial uncoupling protein subfamily and one of the three UCPs (UCP2, UCP4, UCP5), associated with the nervous system. Its putative functions include thermogenesis, attenuation of reactive oxidative species (ROS), regulation of mitochondrial calcium concentration and involvement in cell differentiation and apoptosis. Here we investigate UCP4's subcellular, cellular and tissue distribution, using an antibody designed specially for this study, and discuss the findings in terms of the protein's possible functions. Western blot and immunohistochemistry data confirmed that UCP4 is expressed predominantly in the central nervous system (CNS), as previously shown at mRNA level. No protein was found in heart, spleen, stomach, intestine, lung, thymus, muscles, adrenal gland, testis and liver. The reports revealing UCP4 mRNA in kidney and white adipose tissue were not confirmed at protein level. The amount of UCP4 varies in the mitochondria of different brain regions, with the highest protein content found in cortex. We show that UCP4 is present in fetal murine brain tissue as early as embryonic days 12-14 (E12-E14), which coincides with the beginning of neuronal differentiation. The UCP4 content in mitochondria decreases as the age of mice increases. UCP4 preferential expression in neurons and its developmental expression pattern under physiological conditions may indicate a specific protein function, e.g. in neuronal cell differentiation.
authors: Institute of Cell Biology and Neurobiology, Charité - Universitätsmedizin, Berlin, Germany.
authors: Alina
authors: A
authors: Smorodchenko
authors: Anne
authors: A
authors: Rupprecht
authors: Irina
authors: I
authors: Sarilova
authors: Olaf
authors: O
authors: Ninnemann
authors: Anja U
authors: AU
authors: Bräuer
authors: Kristin
authors: K
authors: Franke
authors: Stefan
authors: S
authors: Schumacher
authors: Sandra
authors: S
authors: Techritz
authors: Robert
authors: R
authors: Nitsch
authors: Markus
authors: M
authors: Schuelke
authors: Elena E
authors: EE
authors: Pohl
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D007473:Ion Channels / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000071245:Mitochondrial Uncoupling Proteins; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D014018:Tissue Distribution
chemical_list: D007074:Immunoglobulin G; D007473:Ion Channels; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D012333:RNA, Messenger; C494853:Slc25a27 protein, rat