title: Evidence for high prevalence of Pneumocystis jirovecii exposure among Cameroonians.
abstract: Cameroon lacks the capacity for routine Pneumocystis pneumonia (PcP) diagnosis, thus, the prevalence of Cameroonian exposure to this microbe is unknown. It is known that Pneumocystis infecting different mammalian host species represent diverse phylogenetic backgrounds and are now designated as separate species. The highly sensitive nature of ELISA and the specificity afforded by using human-derived P. jirovecii Msg peptides has been shown to be useful for serological analysis of human sera. Thus, sera from patients in Yaoundé, the capital city of Cameroon, were analyzed for anti-P. jirovecii antibodies by enzyme-linked immunosorbent assay (ELISA) using three recombinant major surface glycoprotein (Msg) peptide fragments, MsgA1, MsgB, and MsgC1. Based on serum recognition of one or more of the three fragments, 82% of the total samples analyzed was positive for antibodies to P. jirovecii Msg, indicating high prevalence of P. jirovecii infection or colonization among Cameroonians. Different Msg fragments appear to be recognized more frequently by sera from different geographic regions of the globe. Antibodies in the Cameroonian serum samples recognized MsgA1>MsgC1>MsgB, suggesting that different P. jirovecii strains exist in different parts of the world and/or human populations differ in their response to P. jirovecii. Also, HIV(+) patients diagnosed with respiratory infections (such as TB and pneumonia) and maintained on trimethoprim/sulfamethoxazol prophylaxis had relatively lower anti-Msg titers. Whether PcP prophylaxis has significant effects on the quality of life among HIV(+) patients in Cameroon warrants further investigation.
authors: Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221-0006, USA.
authors: Stephenson W
authors: SW
authors: Nkinin
authors: Kieran R
authors: KR
authors: Daly
authors: Peter D
authors: PD
authors: Walzer
authors: Edward S
authors: ES
authors: Ndzi
authors: Tazoacha
authors: T
authors: Asonganyi
authors: Nieves
authors: N
authors: Respaldiza
authors: Francisco J
authors: FJ
authors: Medrano
authors: Edna S
authors: ES
authors: Kaneshiro
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000908:Antibodies, Fungal / Q000097:blood; D000946:Antigens, Fungal; D002163:Cameroon / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015658:HIV Infections / Q000150:complications; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016720:Pneumocystis Infections / Q000453:epidemiology*; D045363:Pneumocystis carinii / Q000276:immunology*; D015995:Prevalence; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal