title: Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain.
abstract: The supermucoid Pseudomonas aeruginosa strain PDO300Deltaalg8(pBBR1MCS-5:alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.
authors: Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
authors: Iain D
authors: ID
authors: Hay
authors: Kali
authors: K
authors: Gatland
authors: Andrea
authors: A
authors: Campisano
authors: J Zoe
authors: JZ
authors: Jordens
authors: Bernd H A
authors: BH
authors: Rehm
mesh_terms: D000464:Alginates; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D017353:Gene Deletion; D005798:Genes, Bacterial; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000235:genetics; D006603:Hexuronic Acids; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase