Characterizing the polymeric status of Helicobacter pylori heat shock protein 60.
Helicobacter pylori heat shock protein 60 (HpHsp60) was first identified as an adhesion molecule associated with H. pylori infection. Here we have analyzed the structure of HpHsp60 via amino acid BLAST, circular dichroism, and electrophoresis and the results indicate that most recombinant HpHsp60 molecules exist as dimers or tetramers, which is quite different from Escherichia coli Hsp60. Treatment of human monocytic cells THP-1 with HpHsp60 was found to up-regulate a panel of cytokines including IL-1alpha, IL-8, IL-10, IFN-gamma, TNF-alpha, TGF-beta, GRO, and RANTES. Carboxymethylated HpHsp60 molecules with a switched oligomeric status were able to further enhance NF-kappaB-mediated IL-8 and TNF-alpha secretion in THP-1 cells compared to unmodified HpHsp60 molecules. These results indicated that the oligomeric status of HpHsp60s might have an important role in regulating host inflammation and thus help facilitate H. pylori persistent infection.
Lin
Ching-Yi
CY
Department of Biological Science and Technology, National Chiao-Tung University, Hsin-Chu, Taiwan.
Huang
Yu-Shan
YS
Li
Chi-Han
CH
Hsieh
Yuan-Ting
YT
Tsai
Nu-Man
NM
He
Pei-Juin
PJ
Hsu
Wei-Tung
WT
Yeh
Yi-Chen
YC
Chiang
Fang-Hsing
FH
Wu
Ming-Shiang
MS
Chang
Chia-Ching
CC
Liao
Kuang-Wen
KW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D018834:Chaperonin 60 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D003545:Cysteine / Q000737:chemistry; D016207:Cytokines / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011108:Polymers / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
C078096:60K protein, Helicobacter pylori; D001426:Bacterial Proteins; D018834:Chaperonin 60; D016207:Cytokines; D011108:Polymers; D011994:Recombinant Proteins; D003545:Cysteine