Microsphere-based co-immunoprecipitation in multiplex.
Co-immunoprecipitation (co-IP) is a prominent technique for evaluating protein-protein interactions. Currently, large quantities of protein are required to perform co-IP followed by mass spectrometric or Western blot analyses of the interacting proteins. Here catenin-cadherin complexes were employed to establish a multiplexed microsphere-based co-immunoprecipitation (micro co-IP) protocol that allows the analysis of different complexes of a given protein with various interacting proteins within a single experiment using a limited amount of sample material.
Poetz
Oliver
O
NMI Natural and Medical Sciences Institute, University of Tübingen, 72770 Reutlingen, Germany. poetz@nmi.de
Luckert
Katrin
K
Herget
Thomas
T
Joos
Thomas O
TO
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods*; D015820:Cadherins / Q000378:metabolism*; D051177:Catenins / Q000378:metabolism*; D047468:Immunoprecipitation / Q000379:methods*; D051379:Mice; D008863:Microspheres; D011485:Protein Binding
D000906:Antibodies; D015820:Cadherins; D051177:Catenins