Immune challenge and satiety-related activation of both distinct and overlapping neuronal populations in the brainstem indicate parallel pathways for viscerosensory signaling.
Caudal brainstem viscerosensory nuclei convey information about the body's internal state to forebrain regions implicated in feeding behavior and responses to immune challenge, and may modulate ingestive behavior following immune activation. Illness-induced appetite loss might be attributed to accentuated "satiety" pathways, activation of a distinct "danger channel" separate from satiety pathways, or both. To evaluate neural substrates that could mediate the effects of illness on ingestive behavior, we analyzed the pattern and phenotypes of medullary neurons responsive to consumption of a preferred food, sweetened milk, and to intraperitoneal lipopolysaccharide challenge that reduced sweetened milk intake. Brainstem sections were stained for c-Fos, dopamine beta-hydroxylase, phenylethanolamine-N-methyltransferase, and glucagon-like peptide-1 (GLP-1) immunoreactivity. Sweetened milk intake activated many neurons throughout the nucleus of the solitary tract (NTS), including A2 noradrenergic neurons in the caudal half of the NTS. LPS challenge activated a similar population of neurons in the NTS, in addition to rostral C2 adrenergic and mid-level A2 noradrenergic neurons in the NTS, many C1 and A1 neurons in the ventrolateral medulla, and in GLP-1 neurons in the dorsal medullary reticular nucleus. Increased numbers of activated GLP-1 neurons in the NTS were only associated with sweetened milk ingestion. Evidence for parallel processing was reflected in the parabrachial nucleus, where sweetened milk intake resulted in activation of the inner external lateral, ventrolateral and central medial portions, whereas LPS challenge induced c-Fos expression in the outer external lateral portions. Thus, signals generated in response to potentially dangerous physiological conditions seem to be propagated via specific populations of catecholaminergic neurons in the NTS and VLM, and likely include a pathway through the external lateral PBN. The data indicate that immune challenge engages multiple ascending neural pathways including both a distinct catecholaminergic "danger" pathway, and a possibly multimodal pathway derived from the NTS.
Gaykema
Ronald P A
RP
Laboratory of Neuroimmunology and Behavior, Center for the Study of Complementary and Alternative Therapies, University of Virginia School of Nursing, P.O. Box 800782 Charlottesville, VA 22908-0782, USA.
Daniels
Teresa E
TE
Shapiro
Nathan J
NJ
Thacker
Gregory C
GC
Park
Su-Mi
SM
Goehler
Lisa E
LE
D000818:Animals; D002452:Cell Count; D004327:Drinking Behavior / Q000502:physiology; D004837:Epinephrine / Q000378:metabolism; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D008526:Medulla Oblongata / Q000276:immunology* / Q000502:physiology*; D009434:Neural Pathways / Q000276:immunology / Q000502:physiology; D009474:Neurons / Q000276:immunology* / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism; D010465:Perception / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012527:Satiation / Q000502:physiology*; D017552:Solitary Nucleus / Q000276:immunology / Q000502:physiology
D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D052216:Glucagon-Like Peptide 1; D009638:Norepinephrine; D004837:Epinephrine