Citrus bergamia Risso & Poiteau juice protects against renal injury of diet-induced hypercholesterolemia in rats.
The present study was designed to evaluate the protective effect of treatment with Citrus bergamia juice (1 mL/day, for 30 days) against hypercholesterolemic diet-induced renal injury in rat.C. bergamia juice provoked a significant reduction in the plasma levels of cholesterol, triglycerides and LDL, and an increase in HDL levels, versus hyperlipidemic controls (p < 0.05). Plasma creatinine levels, measured to assess renal glomerular function, did not change compared with hyperlipidemic controls (0.37 +/- 0.11 mg/dL and 0.32 +/- 0.10 mg/dL, respectively). Moreover, in vivo lipid peroxidation was measured in kidney homogenate; C. bergamia juice administration significantly decreased MDA levels elevations compared with hyperlipidemic controls (4.10 +/- 0.10 nmol/mg protein and 4.78 +/- 0.15 nmol/mg protein, respectively).Histological observations of the kidney supported the biochemical data and indicated a protective effect of C. bergamia juice on the development of renal damage in hypercholesterolemic rats.The antioxidant potential of C. bergamia juice was examined in two in vitro systems: in the DPPH test the juice showed a noticeable effect on scavenging free radicals (IC(50) = 25.01 +/- 0.70 +/-L); in the reducing power assay it showed a strong activity, too (1.44 +/- 0.01 ASE/mL).These findings suggest that C. bergamia juice has a protective role in hypercholesterolemic diet-induced renal damage, which may be attributed to its antioxidant properties.
Trovato
Ada
A
Pharmaco-Biological Department, University of Messina, Vill SS Annunziata, 98168 Messina, Italy.
Taviano
Maria F
MF
Pergolizzi
Simona
S
Campolo
Loredana
L
De Pasquale
Rita
R
Miceli
Natalizia
N
D000818:Animals; D001713:Biphenyl Compounds; D002784:Cholesterol / Q000097:blood; D002957:Citrus / Q000737:chemistry; D003404:Creatinine / Q000097:blood; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000032:analysis; D006937:Hypercholesterolemia / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D014280:Triglycerides; D002784:Cholesterol; D003404:Creatinine; C004931:1,1-diphenyl-2-picrylhydrazyl