Curcumin inhibits ACTH- and angiotensin II-stimulated cortisol secretion and Ca(v)3.2 current.
Adrenocorticotropic hormone and angiotensin II stimulate cortisol secretion from bovine adrenal zona fasciculata cells by the activation of adenylate cyclase and phospholipase C-coupled receptors. Curcumin (1- 20 muM), a compound found in the spice turmeric, inhibited cortisol secretion stimulated by ACTH, AngII, and 8CPT-cAMP. Curcumin also suppressed ACTH-stimulated increases in mRNAs coding for steroid acute regulatory protein and CYP11a1 steroid hydroxylase. In whole cell patch clamp recordings from AZF cells, curcumin at slightly higher concentrations also inhibited Ca(v)3.2 current. These results identify curcumin as an effective inhibitor of ACTH- and AngII-stimulated cortisol secretion. The inhibition of Ca(v)3.2 current by curcumin may contribute to its suppression of secretion.
Enyeart
Judith A
JA
Department of Neuroscience, The Ohio State University College of Medicine and Public Health, Columbus, Ohio 43210-1239, USA.
Liu
Haiyan
H
Enyeart
John J
JJ
D000324:Adrenocorticotropic Hormone / Q000187:drug effects* / Q000378:metabolism; D000804:Angiotensin II / Q000187:drug effects*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020747:Calcium Channels, T-Type / Q000187:drug effects*; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000037:antagonists & inhibitors; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D015394:Molecular Structure
C479952:CACNA1H protein, human; D002121:Calcium Channel Blockers; D020747:Calcium Channels, T-Type; D000804:Angiotensin II; D000324:Adrenocorticotropic Hormone; D002786:Cholesterol Side-Chain Cleavage Enzyme; D003474:Curcumin; D006854:Hydrocortisone