Effect of zona incision by piezo-micromanipulator (ZIP) on in vitro fertilization in 21 transgenic mice lines.
Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is effective for IVF using cryopreserved genetically modified mouse spermatozoa.
Kawase
Yosuke
Y
Chugai Research Institute for Medical Science, Komakado, Gotemba, Shizuoka, Japan.
Tachibe
Takanori
T
Hani
Toshio
T
Tateishi
Hiromi
H
Jishage
Kou-Ichi
K
Suzuki
Hiroshi
H
D000818:Animals; D015925:Cryopreservation; D005260:Female; D005298:Fertility / Q000502:physiology; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D009865:Oocytes / Q000502:physiology*; D012662:Semen Preservation; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*