Lentibacillus jeotgali sp. nov., a halophilic bacterium isolated from traditional Korean fermented seafood.
A novel, Gram-positive, non-motile, endospore-forming and moderately halophilic bacterium, strain Grbi(T), was isolated from a traditional Korean fermented seafood. The organism grew optimally in the presence of 10-15 % NaCl, at 37 degrees C and pH 8.0. The peptidoglycan of the cell wall consisted of meso-diaminopimelic acid, and the predominant menaquinone was MK-7. The major fatty acids of strain Grbi(T) were iso-C(16 : 0) (36.4 %), anteiso-C(15 : 0) (30.3 %) and iso-C(14 : 0) (18.2 %). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. The genomic DNA G+C content was 42.5 mol%. Strain Grbi(T) was most closely related to the type strain Lentibacillus kapialis JCM 12580(T), with which it shared 97.5 % 16S rRNA gene sequence similarity. The DNA-DNA hybridization value between strains Grbi(T) and L. kapialis JCM 12580(T) was 8 %. Based on phenotypic, genotypic and phylogenetic data, strain Grbi(T) should be classified as a novel species within the genus Lentibacillus, for which the name Lentibacillus jeotgali sp. nov. is proposed. The type strain is Grbi(T) (=KCTC 13300(T)=JCM 15795(T)).
Jung
Mi-Ja
MJ
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
Roh
Seong Woon
SW
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
Kim
Min-Soo
MS
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
Bae
Jin-Woo
JW
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
D000818:Animals; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D020459:Genes, rRNA; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D049897:Pectinidae / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017747:Seafood / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride