title: Occult hepatitis B virus infection in a cohort of HIV-positive patients: correlation with hepatitis C virus coinfection, virological and immunological features.
abstract: BACKGROUND
  An evaluation of the prevalence of occult hepatitis B virus (HBV) infection in HIV-positive individuals is important as HBV infection may have an impact on the outcome of the liver disease in these patients.
  
  
  METHODS
  Of the 1,593 HIV-positive subjects enrolled in the Italian Cohort Naïve Antiretroviral (ICONA) program, 175 (10.9%) were selected for inclusion in the study on the basis of hepatitis B surface antigen (HBsAg) negativity and antibody to hepatitis B core antigen (anti- HBc) positivity; 101/175 (58%) were also anti-hepatitis C virus (HCV) positive. HBV-DNA was detected in plasma using a highly sensitive PCR assay (detection limit: 2.6 copies/ml). Two different genomic regions were assayed. Quantification was performed by real-time PCR. The HBV genotype was determined in 20 cases with occult HBV infection. Data on the antiretroviral therapy (ART) regimen was obtained in 169 individuals: 53 (31.4%) patients were ART-naive, 46 (27.2%) were under ART without lamivudine or tenofovir, and the remaining 70 (41.4%) were under ART including lamivudine or tenofovir.
  
  
  RESULTS
  27/175 (15%) patients had detectable HBV-DNA in their plasma: 21/101 (21%) were anti-HCV positive and 6/74 (8%) were anti-HCV negative. Genotype D was invariably found in the 20 cases analyzed. Occult HBV infection was significantly higher in HCV-coinfected subjects: adjusted OR 5.02, 95% CI 1.31-19.26, p = 0.02. The value was not associated with immune status, HIV load, or ART regimen.
  
  
  CONCLUSIONS
  In relation to the high prevalence of occult HBV infection, particularly in HIV/HCV-coinfected individuals, it is necessary to clarify the clinical impact of this cryptic infection by monitoring HBV-DNA in plasma using the correct approach. Similarly to HBsAg-positive individuals of the Mediterranean area, HBV genotype D is invariably detected in this cohort of HIV-infected patients with occult HBV infection.
authors: Dept. of Infectious Diseases, S. Raffaele Scientific Institute, Milan, Italy. morsica.giulia@hsr.it
authors: G
authors: G
authors: Morsica
authors: F
authors: F
authors: Ancarani
authors: S
authors: S
authors: Bagaglio
authors: M
authors: M
authors: Maracci
authors: P
authors: P
authors: Cicconi
authors: A
authors: A
authors: Cozzi Lepri
authors: G
authors: G
authors: Antonucci
authors: R
authors: R
authors: Bruno
authors: T
authors: T
authors: Santantonio
authors: L
authors: L
authors: Tacconi
authors: F
authors: F
authors: Baldelli
authors: R
authors: R
authors: Piscopo
authors: D
authors: D
authors: Santoro
authors: A
authors: A
authors: Lazzarin
authors: A
authors: A
authors: D'Arminio Monforte
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000627:therapeutic use; D015897:Comorbidity; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000150:complications* / Q000188:drug therapy; D006509:Hepatitis B / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018937:Hepatitis C Antibodies / Q000097:blood; D006801:Humans; D007558:Italy; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies