title: Statistical methods for analysis of high-throughput RNA interference screens.
abstract: RNA interference (RNAi) has become a powerful technique for reverse genetics and drug discovery, and in both of these areas large-scale high-throughput RNAi screens are commonly performed. The statistical techniques used to analyze these screens are frequently borrowed directly from small-molecule screening; however, small-molecule and RNAi data characteristics differ in meaningful ways. We examine the similarities and differences between RNAi and small-molecule screens, highlighting particular characteristics of RNAi screen data that must be addressed during analysis. Additionally, we provide guidance on selection of analysis techniques in the context of a sample workflow.
authors: The RNAi Global Initiative, Lafayette, Colorado, USA. amanda.birmingham@thermofisher.com
authors: Amanda
authors: A
authors: Birmingham
authors: Laura M
authors: LM
authors: Selfors
authors: Thorsten
authors: T
authors: Forster
authors: David
authors: D
authors: Wrobel
authors: Caleb J
authors: CJ
authors: Kennedy
authors: Emma
authors: E
authors: Shanks
authors: Javier
authors: J
authors: Santoyo-Lopez
authors: Dara J
authors: DJ
authors: Dunican
authors: Aideen
authors: A
authors: Long
authors: Dermot
authors: D
authors: Kelleher
authors: Queta
authors: Q
authors: Smith
authors: Roderick L
authors: RL
authors: Beijersbergen
authors: Peter
authors: P
authors: Ghazal
authors: Caroline E
authors: CE
authors: Shamu
mesh_terms: D000818:Animals; D003198:Computer Simulation; D015233:Models, Statistical; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics*; D012107:Research Design / Q000706:statistics & numerical data*; D054852:Small Molecule Libraries
chemical_list: D034741:RNA, Small Interfering; D054852:Small Molecule Libraries