title: Development of Schistosoma mansoni in the laboratory rat analyzed by light and confocal laser scanning microscopy.
abstract: The kinetic of maturation (schistogram) of Schistosoma mansoni worms grown in laboratory rats was studied by light and confocal laser scanning microscopy. Infected rats with the BH strain were weekly euthanized 3-9weeks pi. Recovered flukes stained with hydrochloric carmine were preserved as whole-mounts and analyzed by confocal and brightfield microscopy. Worms displayed varying degrees of maturation of the reproductive system at weeks 3-6. Male worms showed complete maturation of the reproductive system at week 6, while female worms completed their maturation at week 7. Males presented few tubercles in tegument in all weeks. Despite the presence of a developing embryo within the ootype, no uterine egg was found. The schistogram in rats follows a pattern similar to that observed in mice hosts.
authors: Laboratory of Helminthology Romero Lascasas Porto, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Biomedical Center, Vila Isabel, 20511-070 Rio de Janeiro, Brazil.
authors: F W
authors: FW
authors: Silva-Leitão
authors: C L
authors: CL
authors: Biolchini
authors: R H
authors: RH
authors: Neves
authors: J R
authors: JR
authors: Machado-Silva
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006790:Host-Parasite Interactions; D008297:Male; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D012550:Schistosoma mansoni / Q000033:anatomy & histology / Q000254:growth & development*; D012555:Schistosomiasis mansoni / Q000469:parasitology*; D012744:Sex Ratio; D012741:Sexual Maturation