title: Systematic antigenic profiling of hematopoietic antigens on ovarian carcinoma cells identifies membrane proteins for targeted therapy development.
abstract: OBJECTIVE
  We studied ovarian cancers for the expression of membrane markers of hematopoietic origin.
  
  
  METHODS
  We used flow cytometry to systematically characterize the expression of more than 30 hematologic antigens on ovarian carcinoma cell lines and to assess their stability under estrogen exposure. The expression of the antigens was validated by a bioinformatics survey and immunohistochemical staining of ovarian cancer specimens.
  
  
  RESULTS
  Several antigens were expressed by the majority of the cells, such as CD15, CD71, and CD138, whereas others were found on small and distinct cellular subpopulations. The expression patterns of the different markers were unaffected by estrogen exposure, indicating their stability.
  
  
  CONCLUSIONS
  The antigens described in our work may serve as potential targets for new and existing targeted drugs.
authors: Department of Obstetrics and Gynecology, Tel Aviv Sourasky Medical Center, Tel-Aviv, Israel.
authors: Limor
authors: L
authors: Helpman
authors: Ben-Zion
authors: BZ
authors: Katz
authors: Tamar
authors: T
authors: Safra
authors: Letizia
authors: L
authors: Schreiber
authors: Zurit
authors: Z
authors: Levine
authors: Sergey
authors: S
authors: Nemzer
authors: Yaron
authors: Y
authors: Kinar
authors: Dan
authors: D
authors: Grisaru
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D019295:Computational Biology; D015195:Drug Design; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006410:Hematopoiesis; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000628:therapy*; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism; D053668:Syndecan-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; C068126:CD71 antigen; D016256:Lewis X Antigen; D011990:Receptors, Transferrin; C508241:SDC1 protein, human; D053668:Syndecan-1