title: Evaluation of sanitizing efficacy of acetic acid on Piper betle leaves and its effect on antioxidant properties.
abstract: The sanitizing efficacy of acetic acid and its effect on health beneficial properties of Piper betle leaves were determined. Betel leaves artificially inoculated with Aeromonas, Salmonella and Yersinia were subjected to organic acid (citric acid, acetic acid and lactic acid) treatment. Pathogen populations reduced by 4 log upon individual inoculation and up to 2 log in a mixed cocktail following treatment with 2% acetic acid during storage up to 20 h at 28 degrees C, indicating a residual antimicrobial effect on pathogen during storage. Antioxidant potential ethanolic extracts of both raw and treated P. betle leaves were assayed for free radical scavenging activities against 2,2-diphenyl-1-picryhydrazyl. Polyphenols, flavonoids and the reducing power of treated and untreated P. betle were also compared. No significant (P>0.05) changes were observed in antioxidant status; flavonoids, polyphenols and reducing power of treated betel leaves. Results indicate the feasibility of a simple intervention strategy for inactivating pathogens in edible leaves of P. betle.
authors: Department of Biotechnology and Environmental Sciences, Thapar University, Patiala, Punjab, India.
authors: Richu
authors: R
authors: Singla
authors: Abhijit
authors: A
authors: Ganguli
authors: Moushumi
authors: M
authors: Ghosh
authors: Sapna
authors: S
authors: Sohal
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D000333:Aeromonas / Q000187:drug effects / Q000302:isolation & purification; D000975:Antioxidants / Q000032:analysis*; D019343:Citric Acid / Q000494:pharmacology; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D005419:Flavonoids / Q000032:analysis; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D016166:Free Radical Scavengers / Q000032:analysis; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis; D031721:Piper betle / Q000737:chemistry / Q000382:microbiology*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000382:microbiology*; D059808:Polyphenols; D012475:Salmonella / Q000187:drug effects / Q000302:isolation & purification; D012677:Sensation; D013045:Species Specificity; D013997:Time Factors; D015007:Yersinia / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D004202:Disinfectants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D019343:Citric Acid; D019344:Lactic Acid; D019342:Acetic Acid