Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells.
Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. In particular we consider the effect of a non spatially coherent source on the phase-image signal-to-noise ratio. Precise measurements of the phase-shift introduced by microscopic beads or giant unilamellar vesicles validate the principle and show the accuracy of the methods. Diffraction limited images of living COS-7 cells are then presented, with a particular focus on the membrane and organelle dynamics.
Bon
Pierre
P
Aix-Marseille Université, Ecole Centrale Marseille, CNRS, Institut Fresnel, Campus de Saint-Jérôme, 13013 Marseille, France. pierre.bon@fresnel.fr
Maucort
Guillaume
G
Wattellier
Benoit
B
Monneret
Serge
S
D000465:Algorithms; D000818:Animals; D019556:COS Cells; D018929:Cell Culture Techniques / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D004574:Electromagnetic Fields; D004867:Equipment Design; D007368:Interferometry / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D015233:Models, Statistical; D055095:Optics and Photonics; D015388:Organelles / Q000378:metabolism