Mammalian reoviruses: propagation, quantification, and storage.
Mammalian reoviruses are pathogens that cause gastrointestinal and respiratory infections. In humans, the mammalian reoviruses usually cause mild or subclinical disease, and they are ubiquitous, with most people mounting immunity at a young age. Reoviruses are prototypic representations of the Reoviridae family, which contains many highly pathogenic viruses. This unit describes techniques for culturing mouse fibroblast L929 cell lines, the preferred cell line in which most mammalian reovirus studies take place. In addition, mammalian reovirus propagation, quantification, purification, and storage are described.
Berard
Alicia
A
University of Manitoba and Manitoba Centre for Proteomics and Systems Biology, Winnipeg, Manitoba, Canada.
Coombs
Kevin M
KM
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D003264:Containment of Biohazards; D051379:Mice; D011309:Preservation, Biological / Q000379:methods; D012087:Reoviridae / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods; D010948:Viral Plaque Assay / Q000379:methods; D014776:Virus Cultivation / Q000379:methods*