MOV10 as a novel telomerase-associated protein.
A novel telomerase-associated protein was isolated from porcine testis. The 115-kDa protein, purified with telomerase activity, was molecular cloned using human cDNA library, and identified as MOV10. The expression levels of both MOV10 mRNA and MOV10 protein in cancer cells were 2-3 times higher than that of the normal cells, and MOV10 mRNA was highly expressed in human testis and ovary. The anti-MOV10 antibody precipitated the telomerase activity from cancer cell extracts, and inhibited the telomerase activity in vitro. Sf9-expressed MOV10 protein bound to G-rich strand of both single- and double-stranded telomere-sequenced DNA, but not to single C-rich strand. ChIP assay showed the binding of MOV10 to telomere region in vivo. These data suggest that MOV10 is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.
Nakano
Mariko
M
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo 112-8610, Japan.
Kakiuchi
Yasutaka
Y
Shimada
Yukiko
Y
Ohyama
Mika
M
Ogiwara
Yasuko
Y
Sasaki-Higashiyama
Narie
N
Yano
Naoko
N
Ikeda
Fumi
F
Yamada
Ei
E
Iwamatsu
Akihiro
A
Kobayashi
Kazuo
K
Nishiyama
Kiyoto
K
Ichikawa
Sohsuke
S
Kaji
Kazuhiko
K
Ide
Toshinori
T
Murofushi
Hiromu
H
Murakami-Murofushi
Kimiko
K
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D008297:Male; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000201:enzymology*; D013737:Testis / Q000201:enzymology
D004247:DNA; C530476:Mov10 protein, human; D019098:Telomerase; D020365:RNA Helicases