Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis.
BACKGROUND
Timely identification of pathogens is crucial to minimize mortality in patients with severe infections. Detection of bacterial and fungal pathogens in blood by nucleic acid amplification promises to yield results faster than blood cultures (BC). We analyzed the clinical impact of a commercially available multiplex PCR system in patients with suspected sepsis.


METHODS
Blood samples from patients with presumed sepsis were cultured with the Bactec 9240 system (Becton Dickinson, Heidelberg, Germany) and aliquots subjected to analysis with the LightCycler SeptiFast (SF) Test (Roche Diagnostics, Mannheim, Germany) at a tertiary care centre. For samples with PCR-detected pathogens, the actual impact on clinical management was determined by chart review. Furthermore a comparison between the time to a positive blood culture result and the SF result, based on a fictive assumption that it was done either on a once or twice daily basis, was made.


RESULTS
Of 101 blood samples from 77 patients, 63 (62%) yielded concordant negative results, 14 (13%) concordant positive and 9 (9%) were BC positive only. In 14 (13%) samples pathogens were detected by SF only, resulting in adjustment of antibiotic therapy in 5 patients (7,7% of patients). In 3 samples a treatment adjustment would have been made earlier resulting in a total of 8 adjustments in all 101 samples (8%).


CONCLUSIONS
The addition of multiplex PCR to conventional blood cultures had a relevant impact on clinical management for a subset of patients with presumed sepsis.
Dierkes
Christine
C
Department of Internal Medicine I, University Hospital Regensburg, 93042 Regensburg, Germany. Christine.dierkes@klinik.uni-r.de
Ehrenstein
Boris
B
Siebig
Sylvia
S
Linde
Hans-Jörg
HJ
Reischl
Udo
U
Salzberger
Bernd
B
D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000628:therapy; D004269:DNA, Bacterial / Q000032:analysis; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009181:Mycoses / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000628:therapy; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D018805:Sepsis / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology / Q000628:therapy
D004269:DNA, Bacterial; D004271:DNA, Fungal