Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori.
A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line.
Pan
Min-Hui
MH
The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, 216 Tiansheng Rd., Beibei District, Chongqing 400716, China.
Cai
Xiu-Juan
XJ
Liu
Min
M
Lv
Jun
J
Tang
Hui
H
Tan
Juan
J
Lu
Cheng
C
D000818:Animals; D012831:Bombyx / Q000166:cytology* / Q000502:physiology / Q000821:virology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D004171:Diploidy; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology / Q000821:virology; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity; D010053:Ovary / Q000166:cytology* / Q000502:physiology / Q000821:virology; D014162:Transfection / Q000379:methods
D049452:Green Fluorescent Proteins