title: Permethrin induces overexpression of cytochrome c oxidase subunit 3 in Aedes aegypti.
abstract: Using quantitative polymerase chain reaction (QPCR), the relative transcriptional levels of cytochrome c oxidase subunit 3 (CO3) were studied in Aedes aegypti in response to treatment with acetone, permethrin, and fipronil. The transcriptional levels of CO3 were significantly (P < 0.05) higher in acetone-treated Ae. aegypti compared with that in untreated samples. Using ribosomal L24, heat shock protein (HSP), and actin as reference genes, relative transcription levels of CO3 in acetone-treated Ae. aegypti were 2.88 +/- 0.38-, 2.60 +/- 0.60-, and 3.24 +/- 0.70-fold higher, respectively, compared with that in untreated mosquitoes. Transcriptional levels of CO3 were induced significantly higher (6.54 +/- 1.22-, 4.62 +/- 0.74-, and 9.47 +/- 3.71-fold, respectively) by permethrin at LD10 compared with acetone (P < 0.05). Taken together, our results suggest that overexpression of CO3 is tightly regulated in Ae. aegypti in response to xenobiotic treatment.
authors: Center for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, 1600 SW, 23rd Dr., Gainesville, FL 32608, USA. Julia.Pridgeon@ars.usda.gov
authors: Julia W
authors: JW
authors: Pridgeon
authors: James J
authors: JJ
authors: Becnel
authors: Gary G
authors: GG
authors: Clark
authors: Kenneth J
authors: KJ
authors: Linthicum
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D007306:Insecticides / Q000494:pharmacology*; D026023:Permethrin / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D007306:Insecticides; D011720:Pyrazoles; D012333:RNA, Messenger; D026023:Permethrin; D003576:Electron Transport Complex IV; C082360:fipronil