Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain.
The supermucoid Pseudomonas aeruginosa strain PDO300Deltaalg8(pBBR1MCS-5:alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.
Hay
Iain D
ID
Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
Gatland
Kali
K
Campisano
Andrea
A
Jordens
J Zoe
JZ
Rehm
Bernd H A
BH
D000464:Alginates; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D017353:Gene Deletion; D005798:Genes, Bacterial; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000235:genetics; D006603:Hexuronic Acids; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase