title: Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis.
abstract: A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.
authors: Medical Research Center for Gene Regulation and Department of Pharmacology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors: Gwang Hyeon
authors: GH
authors: Eom
authors: Kabsun
authors: K
authors: Kim
authors: Sung-Mi
authors: SM
authors: Kim
authors: Hae Jin
authors: HJ
authors: Kee
authors: Ji-Young
authors: JY
authors: Kim
authors: Hye Mi
authors: HM
authors: Jin
authors: Ju-Ryoung
authors: JR
authors: Kim
authors: Jung Ha
authors: JH
authors: Kim
authors: Nakwon
authors: N
authors: Choe
authors: Kee-Beom
authors: KB
authors: Kim
authors: Junwon
authors: J
authors: Lee
authors: Hyun
authors: H
authors: Kook
authors: Nacksung
authors: N
authors: Kim
authors: Sang-Beom
authors: SB
authors: Seo
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000254:growth & development*; D014018:Tissue Distribution; D014158:Transcription, Genetic; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D012739:Gonadal Steroid Hormones; D012097:Repressor Proteins; D000076983:Histone Methyltransferases; C543643:PRDM8 protein, mouse; D011495:Histone-Lysine N-Methyltransferase