Use of polyamidoamine dendrimers to engineer BDNF-producing human mesenchymal stem cells.
We report the use of polyamidoamine (PAMAM-NH(2)) dendrimers along with other non-viral vehicles for the in vitro transfection of human bone marrow mesenchymal stem cells (hMSCs) and for engineering MSCs to secrete brain-derived neurotrophic factor (BDNF). Different generations of cationic polyamidoamine dendrimers (generations 3-6) were tested on HEK 293T cells. hMSCs were then transfected with PAMAM-NH(2) G4 dendrimers and Lipofectamine 2000, which elicited the expression of GFP reporter in around 6 and 20% of the cells, respectively. Both vehicles were then shown to elicit the expression of BDNF in MSCs from a bicistronic cassette. Non-virally induced neurotrophin expression may be a safe and easy method for adapting autologous stem cells for therapeutic treatment of diseases and neural system injuries.
Shakhbazau
Antos
A
Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Akademicheskaya, 27, 220072, Minsk, Belarus. shakhbazau@gmail.com
Shcharbin
Dzmitry
D
Seviaryn
Ihar
I
Goncharova
Natalya
N
Kosmacheva
Svetlana
S
Potapnev
Mihail
M
Gabara
Barbara
B
Ionov
Maxim
M
Bryszewska
Maria
M
D000328:Adult; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D050091:Dendrimers / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D019076:Transgenes / Q000235:genetics
D019208:Brain-Derived Neurotrophic Factor; D050091:Dendrimers; C104700:PAMAM Starburst; D004247:DNA