A simple twist for signal enhancement in non-linear optical microscopy.
We describe a very simple but elegant approach to two-photon fluorescence signal enhancement by intensity modulation with immediate application in two-photon laser-scanning fluorescence microscopy. This method of enhancement shows potential application in any microscopic technique that result from non-linear photon absorption and plays a pivotal role in live cell imaging.
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Department of Chemistry, Indian Institute of Technology Kanpur, UP-208016, India.
Goswami
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D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000648:ultrastructure; D042783:Endothelial Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D014404:Tubulin / Q000648:ultrastructure
D014404:Tubulin