[Isolation and identification of Malassezia species isolated from healthy skin of malnourished and eutrophic children cared for in daycare centers in Venezuela].
The Malassezia genus contains a group of lipophilic yeasts that form part of the normal microbiota of human and animals skins. The presence of Malassezia species was studied on healthy skin in children, who attended day care centers, located in Maracaibo, Zulia state, Venezuela. Children without skin lesions that were anthropometrically classified as malnourished (48) and eutrophics (40), were studied. Samples were taken from different anatomic regions with a transparent plastic tape. The direct examination was done using blue methylene (0.25%) and then, samples were cultivated in Dixon medium with antibiotic. The identification of the isolated yeasts was done by classical methodology. The results obtained indicate that malnourished and eutrophics children were positive to Malassezia in relatively high prevalences (66.6% and 65.0% respectively). It is worthy of notice that M. furfur was predominantly present in malnourished children (77%). It was isolated from all the anatomic locations in both groups of children. M. slooffiae was mainly located in the back of the eutrophic children. M. furfur was isolated preferently in malnourished male children, while in the eutrophic children, it was observed in females. It was demonstrated that M. furfur was predominantly present in the healthy skin of malnourished and eutrophic children.
González-Morán
Evelyn
E
Laboratorio de Micología, Escuela de Bioanálisis, Universidad del Zulia, Maracaibo, Venezuela.
Rodríguez-Valero
Sofía
S
Del Monte
María L
ML
Briceño
Marlin
M
Sintjago
Skarleth
S
Mesa
Luz Mila
LM
García
Doris
D
Villalobos
Rafael
R
Pereira
Nayda
N
D001827:Body Height; D001835:Body Weight; D002648:Child; D002656:Child Day Care Centers; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008290:Malassezia / Q000145:classification / Q000302:isolation & purification*; D008297:Male; D044342:Malnutrition / Q000382:microbiology*; D009928:Organ Specificity; D012737:Sex Factors; D012867:Skin / Q000382:microbiology*; D013045:Species Specificity; D014505:Urban Population; D014687:Venezuela / Q000453:epidemiology