title: YidC is required for the assembly of the MscL homopentameric pore.
abstract: The mechanosensitive channel with large conductance (MscL) of Escherichia coli is formed by a homopentameric assembly of MscL proteins. Here, we describe MscL biogenesis as determined using in vivo approaches. Evidence is presented that MscL is targeted to the inner membrane via the signal recognition particle (SRP) pathway, and is inserted into the lipid bilayer independently of the Sec machinery. This is consistent with published data. Surprisingly, and in conflict with earlier data, YidC is not critical for membrane insertion of MscL. In the absence of YidC, assembly of the homopentameric MscL complex was strongly reduced, suggesting a late role for YidC in the biogenesis of MscL. The data are consistent with the view that YidC functions as a membrane-based chaperone 'module' to facilitate assembly of a subset of protein complexes in the inner membrane of E. coli.
authors: Section Molecular Microbiology, Department of Molecular Cell Biology, VU University, Amsterdam, The Netherlands.
authors: Ovidiu I
authors: OI
authors: Pop
authors: Zora
authors: Z
authors: Soprova
authors: Gregory
authors: G
authors: Koningstein
authors: Dirk-Jan
authors: DJ
authors: Scheffers
authors: Peter
authors: P
authors: van Ulsen
authors: David
authors: D
authors: Wickström
authors: Jan-Willem
authors: JW
authors: de Gier
authors: Joen
authors: J
authors: Luirink
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D007425:Intracellular Membranes / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D055503:Protein Multimerization; D018271:Signal Recognition Particle / Q000502:physiology
chemical_list: D029968:Escherichia coli Proteins; D007473:Ion Channels; D026901:Membrane Transport Proteins; C086596:MscL protein, E coli; D018271:Signal Recognition Particle; C412443:YIDC protein, E coli