title: Virgibacillus byunsanensis sp. nov., isolated from a marine solar saltern.
abstract: A Gram-variable, motile, endospore-forming and rod-shaped bacterial strain, ISL-24(T), was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by a polyphasic study. Strain ISL-24(T) grew optimally at pH 7.0-8.0, at 30-37 degrees C and in the presence of 8 % (w/v) NaCl. It contained MK-7 as the predominant menaquinone and anteiso-C(15 : 0) as the predominant fatty acid. The DNA G+C content was 37.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-24(T) fell within the genus Virgibacillus, clustering with Virgibacillus carmonensis LMG 20964(T) and Virgibacillus necropolis LMG 19488(T), with a bootstrap resampling value of 92.3 %, and exhibiting 97.3 and 97.4 % 16S rRNA gene sequence similarity, respectively, to these strains. Strain ISL-24(T) exhibited 94.8-96.8 % 16S rRNA gene sequence similarity to the type strains of the other Virgibacillus species. Mean DNA-DNA relatedness values between strain ISL-24(T) and V. carmonensis DSM 14868(T) and V. necropolis DSM 14866(T) were 11 and 19 %, respectively. Differential phenotypic properties of strain ISL-24(T), together with the phylogenetic and genetic distinctiveness, revealed that this strain is different from recognized Virgibacillus species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-24(T) represents a novel species of the genus Virgibacillus, for which the name Virgibacillus byunsanensis sp. nov. is proposed. The type strain is ISL-24(T) (=KCTC 13259(T) =CCUG 56754(T)).
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors: So-Jung
authors: SJ
authors: Kang
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors: Yong-Taek
authors: YT
authors: Jung
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors: Keun Chul
authors: KC
authors: Lee
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors: Hyun Woo
authors: HW
authors: Oh
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors: Tae-Kwang
authors: TK
authors: Oh
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000502:physiology; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007723:Korea; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000737:chemistry; D012623:Seawater / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts