Crystallization and preliminary crystallographic analysis of the bacterial capsule assembly-regulating tyrosine phosphatases Wzb of Escherichia coli and Cps4B of Streptococcus pneumoniae.
Bacterial tyrosine kinases and their cognate phosphatases are key players in the regulation of capsule assembly and thus are important virulence determinants of these bacteria. Examples of the kinase/phosphatase pairing are found in Gram-negative bacteria such as Escherichia coli (Wzc and Wzb) and in Gram-positive bacteria such as Streptococcus pneumoniae (CpsCD and CpsB). Although Wzb and Cps4B are both predicted to dephosphorylate the C-terminal tyrosine cluster of their cognate tyrosine kinase, they appear on the basis of protein sequence to belong to quite different enzyme classes. Recombinant purified proteins Cps4B of S. pneumoniae TIGR4 and Wzb of E. coli K-30 have been crystallized. Wzb crystals belonged to space-group family P3(x)21 and diffracted to 2.7 A resolution. Crystal form I of Cps4B belonged to space-group family P4(x)2(1)2 and diffracted to 2.8 A resolution; crystal form II belonged to space group P2(1)2(1)2(1) and diffracted to 1.9 A resolution.
Huang
Hexian
H
Centre for Biomolecular Sciences, The University of St Andrews, Fife KY16 9RH, Scotland.
Hagelueken
Gregor
G
Whitfield
Chris
C
Naismith
James H
JH
D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D013296:Streptococcus pneumoniae / Q000201:enzymology*
D017931:DNA Primers; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D010749:Phosphoprotein Phosphatases; C428435:Wzb protein, E coli; D017027:Protein Tyrosine Phosphatases