Nitrophenolates spray can alter boll abscission rate in cotton through enhanced peroxidase activity and increased ascorbate and phenolics levels.
Field studies were conducted from 2002 to 2005 to evaluate foliar spray of Atonik (a plant growth regulator (PGR) containing nitrophenolates) on cotton boll abscission rate by assessing various reactive oxygen species (ROS) contents, antioxidant content and antioxidant enzyme activity from 1 to 9 days after anthesis (DAA). The result indicated that the nitrophenolate spray reduced hydrogen peroxide (H(2)O(2)), superoxide anion (O(2)(-)) accumulation, lipid peroxidation (malondialdehyde--MDA), lipoxygenase (LOX) activity and membrane permeability relative to the control. Antioxidant enzyme activity (superoxide dismutase, SOD; ascorbate peroxidase, APX; peroxidase, POX; glutathione peroxidase, GSH-Px) was significantly increased by the nitrophenolate spray. The POX (217%) and GSH-Px (242%) activities were enhanced compared with APX (7.7%) activity at 9 DAA. Enhanced accumulation of ascorbate (245%), phenol (253%) and proline (150%) was observed in nitrophenolate-sprayed plants compared with control at 9 DAA. Because ascorbate content is increased by higher dehydroascorbate reductase (DHAR) enzyme activity, the ascorbate was able to replenish reducing equivalents to phenoxyl radicals, resulting in an increase of phenolic compounds. The increased phenolic acid content may be involved in scavenging the ROS produced in developing cotton boll. The role of DHAR and glutathione reductase (GR) in keeping higher levels of reduced ascorbate and low levels of endogenous H(2)O(2) in the developing cotton boll may be the prerequisite for boll retention. Based on the present work, we conclude that nitrophenolate-sprayed plants counteracted the deleterious effects of ROS by the peroxide/phenolics/ascorbate system, which causes reduced boll abscission and increased yield.
Djanaguiraman
M
M
Department of Crop Physiology, Tamil Nadu Agricultural University, India. djanaguiraman@yahoo.co.in
Sheeba
J Annie
JA
Devi
D Durga
DD
Bangarusamy
U
U
Prasad
P V V
PV
D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003683:Dehydroascorbic Acid / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D003368:Gossypium / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008084:Lipoxygenase / Q000378:metabolism; D009596:Nitrophenols / Q000378:metabolism / Q000494:pharmacology*; D016877:Oxidants / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000033:anatomy & histology / Q000187:drug effects; D011392:Proline / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D009596:Nitrophenols; D016877:Oxidants; D013481:Superoxides; D011392:Proline; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D008084:Lipoxygenase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; C020666:glutathione dehydrogenase (ascorbate); D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid