title: Molecular epidemiology of Brucella genotypes in patients at a major hospital in central Peru.
abstract: The multiple-locus variable-number repeat analysis of 90 human Brucella melitensis isolates from a large urban area in central Peru revealed variations at 4 (Bruce07, Bruce09, Bruce18, and Bruce42) out of 16 loci investigated, of which 1 (Bruce42) also is used for species identification. Ten genotypes were identified, separated by the number of Bruce42 repeats into two groups that may have distinct phenotypic characteristics. Whereas genotypes with five or six Bruce42 repeats were cultured mainly from adult patients, genotypes with three Bruce42 repeats were isolated from children and young adolescents as well as from adults. In addition, the isolates with three Bruce42 repeats were obtained more often from patients with splenomegaly (P = 0.02) or hepatomegaly (P = 0.006). An annual variation in the diversity of genotypes was observed, possibly reflecting changes in sources of fresh dairy products, supply routes to city shops and markets, and the movement of infected dairy goat herds.
authors: Federal Institute for Risk Assessment, Berlin, Germany.
authors: Karsten
authors: K
authors: Nöckler
authors: Ryan
authors: R
authors: Maves
authors: David
authors: D
authors: Cepeda
authors: Angelika
authors: A
authors: Draeger
authors: Anne
authors: A
authors: Mayer-Scholl
authors: Jesus
authors: J
authors: Chacaltana
authors: María
authors: M
authors: Castañeda
authors: Benjamin
authors: B
authors: Espinosa
authors: Rosa
authors: R
authors: Castillo
authors: Eric
authors: E
authors: Hall
authors: Sascha
authors: S
authors: Al Dahouk
authors: Robert H
authors: RH
authors: Gilman
authors: Franco
authors: F
authors: Cabeza
authors: Henk L
authors: HL
authors: Smits
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D017347:Brucella melitensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000453:epidemiology* / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D003611:Dairy Products / Q000382:microbiology; D005838:Genotype; D006041:Goats / Q000382:microbiology; D006761:Hospitals; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D010568:Peru / Q000453:epidemiology; D011110:Polymorphism, Genetic; D014505:Urban Population
chemical_list: D004269:DNA, Bacterial