Prototheca cutis sp. nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin.
A strain of a novel pathogenic, achlorophyllic alga belonging to the genus Prototheca was isolated from the inflamed skin of a patient with protothecosis in a Japanese hospital. The pathogen was detected and isolated in biopsy specimens by histopathology and culture-based examination. Analyses of the nuclear 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences and chemotaxonomic studies indicated that this strain represents a novel species with a close phylogenetic relationship to Prototheca wickerhamii and Auxenochlorella protothecoides. This strain grew well at 28-30 degrees C, showed slow and weak growth at 37 degrees C, and no growth at 40 degrees C. This strain grew in vitamin-free medium and assimilated acetate (pH 5.1), l-arabinose and soluble starch as a carbon source. The taxonomic description of Prototheca cutis sp. nov. is proposed (type strain JCM 15793(T) =CBS 11262(T) =DSM 22084(T)) as a pathogen of dermatitis.
Satoh
Kazuo
K
Japan Health Sciences Foundation, Chuo-ku, Tokyo, Japan.|Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan.
Ooe
Kenji
K
Asahi General Hospital, Asahi, Chiba, Japan.
Nagayama
Hirotoshi
H
Asahi General Hospital, Asahi, Chiba, Japan.
Makimura
Koichi
K
Genome Research Center, Graduate School of Medicine and Faculty of Medicine, Teikyo University, Hachioji, Tokyo, Japan.|Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan.
D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D003872:Dermatitis / Q000382:microbiology* / Q000473:pathology; D020459:Genes, rRNA; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011525:Prototheca / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology* / Q000473:pathology; D012874:Skin Diseases, Infectious / Q000382:microbiology* / Q000473:pathology; D013045:Species Specificity
D003470:Culture Media; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C065096:RNA, ribosomal, 26S