title: Toward a microscopic model of bidirectional synaptic plasticity.
abstract: We show that a 2-step phospho/dephosphorylation cycle for the alpha-amino-3-hydroxy-5-methyl-4-isoxazole proprionic acid receptor (AMPAR), as used in in vivo learning experiments to assess long-term potentiation (LTP) induction and establishment, exhibits bistability for a wide range of parameters, consistent with values derived from biological literature. The AMPAR model we propose, hence, is a candidate for memory storage and switching behavior at a molecular-microscopic level. Furthermore, the stochastic formulation of the deterministic model leads to a mesoscopic interpretation by considering the effect of enzymatic fluctuations on the Michelis-Menten average dynamics. Under suitable hypotheses, this leads to a stochastic dynamical system with multiplicative noise whose probability density evolves according to a Fokker-Planck equation in the Stratonovich sense. In this approach, the probability density associated with each AMPAR phosphorylation state allows one to compute the probability of any concentration value, whereas the Michaelis-Menten equations consider the average concentration dynamics. We show that bistable dynamics are robust for multiplicative stochastic perturbations and that the presence of both noise and bistability simulates LTP and long-term depression (LTD) behavior. Interestingly, the LTP part of this model has been experimentally verified as a result of in vivo, one-trial inhibitory avoidance learning protocol in rats, that produced the same changes in hippocampal AMPARs phosphorylation state as observed with in vitro induction of LTP with high-frequency stimulation (HFS). A consequence of this model is the possibility of characterizing a molecular switch with a defined biochemical set of reactions showing bistability and bidirectionality. Thus, this 3-enzymes-based biophysical model can predict LTP as well as LTD and their transition rates. The theoretical results can be, in principle, validated by in vitro and in vivo experiments, such as fluorescence measurements and electrophysiological recordings at multiple scales, from molecules to neurons. A further consequence is that the bistable regime occurs only within certain parametric windows, which may simulate a "history-dependent threshold". This effect might be related to the Bienenstock-Cooper-Munro theory of synaptic plasticity.
authors: Department of Physics and National Institute of Nuclear Physics, University of Bologna, 40126 Bologna, Italy.
authors: Gastone C
authors: GC
authors: Castellani
authors: Armando
authors: A
authors: Bazzani
authors: Leon N
authors: LN
authors: Cooper
mesh_terms: D000465:Algorithms; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006624:Hippocampus / Q000502:physiology*; D007700:Kinetics; D017774:Long-Term Potentiation / Q000502:physiology*; D036881:Long-Term Synaptic Depression / Q000502:physiology; D008954:Models, Biological; D008959:Models, Neurological; D009010:Monte Carlo Method; D009473:Neuronal Plasticity / Q000502:physiology*; D010766:Phosphorylation; D011336:Probability; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism
chemical_list: D018091:Receptors, AMPA; D002118:Calcium