Avian reoviruses: propagation, quantification, and storage.
Avian reoviruses (ARVs) are pathogens that cause significant morbidity among commercial poultry. ARVs are prototypic representatives of non-enveloped viruses that can cause cell-cell fusion. They belong to the Reoviridae family, which contains many highly pathogenic viruses. ARVs are ubiquitous in commercial poultry and are frequently isolated from the gastrointestinal and respiratory tracts of chickens with acute infections. The virus causes a range of disease states in chicken, including viral arthritis/tenosynovitis, gastroenteritis, hepatitis, myocarditis, "pale bird syndrome," runting-stunting syndrome, and respiratory illness. This unit describes avian reovirus propagation, quantification, and storage.
Tran
Anh
A
University of Manitoba and Manitoba Centre for Proteomics and Systems Biology, Winnipeg, Manitoba, Canada.
Berard
Alicia
A
Coombs
Kevin M
KM
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D045744:Cell Line, Tumor; D003264:Containment of Biohazards; D030101:Orthoreovirus, Avian / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D011309:Preservation, Biological / Q000379:methods; D011784:Quail; D013194:Staining and Labeling / Q000379:methods; D010948:Viral Plaque Assay / Q000379:methods; D014776:Virus Cultivation / Q000379:methods*