title: The Karimojong from Uganda: genetic characterization using an X-STR decaplex system.
abstract: The Karimojong, an African group from the Karamoja region of Northeast Uganda, were genetically analysed using a decaplex system for X chromosome short tandem repeats (X-STRs). A total of 255 individuals (117 males and 138 females) were genotyped for the following loci: DXS8378, DXS9898, DXS7133, GATA31E08, GATA172D05, DXS7423, DXS6809, DXS7132, DXS9902 and DXS6789. Allele frequencies and parameters for forensic evaluation were calculated for each STR. No association was found between any pairs of loci studied. DXS6789 was the most polymorphic marker in this sample, followed by DXS6809, with gene diversities of 84.79% and 83.94%, respectively. The less discriminating locus observed was DXS7133, with a gene diversity of 39.79%. High overall values of power of discrimination were obtained for female (1 in 1.8 x 10(10)) and male samples (1 in 1.6 x 10(6)), as well as high power of exclusion in father/mother/daughter trios (99.9997%), in father daughter duos (99.9862%) and in half sisters with same father (99.0331%). These results confirm the potential of this 10-plex in parentage testing and in human identification.
authors: IPATIMUP, Institute of Pathology and Molecular Immunology, University of Porto, 4200-465 Porto, Portugal.
authors: Iva
authors: I
authors: Gomes
authors: Vânia
authors: V
authors: Pereira
authors: Verónica
authors: V
authors: Gomes
authors: Maria João
authors: MJ
authors: Prata
authors: Nádia
authors: N
authors: Pinto
authors: Angel
authors: A
authors: Carracedo
authors: António
authors: A
authors: Amorim
authors: Leonor
authors: L
authors: Gusmão
mesh_terms: D000483:Alleles; D044383:Black People / Q000235:genetics; D041321:Chromosomes, Human, X; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011786:Quality Control; D014454:Uganda
chemical_list: D004247:DNA