title: Study of the immunological profile towards Mycobacterium bovis antigens in naturally infected cattle.
abstract: A number of studies have determined the contribution of Th1 and Th2 responses to the protective immunity and pathology of Mycobacterium bovis infection. However, much of that information is derived from experimentally infecting cattle with M. bovis and few data from naturally infected animals are available. The aim of this study was to characterize the immunological profile towards M. bovis antigens of naturally infected cattle by measurement of cytokine mRNA expression in PBMC, and to determine which lymphocyte subsets are involved in recall responses of PBMC from M. bovis infected cattle to M. bovis antigens. Consistent with data from cattle experimentally infected with M. bovis, naturally infected animals were found to display a Th1 cytokine profile in response to M. bovis PPDB stimulation. Production of IFN-gamma mRNA by PBMC after PPDB stimulation statistically distinguishes between infected and healthy herds, suggesting that this molecule is usable as an M. bovis-infection marker. As happens in experimentally infected cows, CD4, CD8 and gammadeltaTCR cells from a herd naturally infected with M. bovis are the predominant T cell subsets expanded in response to PPDB.
authors: Institute of Biotechnology, Centre of Agriculture and Veterinary Research, National Institute for Agriculture, Buenos Aires, Argentina.
authors: Federico C
authors: FC
authors: Blanco
authors: Pablo
authors: P
authors: Schierloh
authors: María Verónica
authors: MV
authors: Bianco
authors: Karina
authors: K
authors: Caimi
authors: Virginia
authors: V
authors: Meikle
authors: Alicia E
authors: AE
authors: Alito
authors: Angel A
authors: AA
authors: Cataldi
authors: María Del Carmen
authors: Mdel C
authors: Sasiain
authors: Fabiana
authors: F
authors: Bigi
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014380:Tuberculosis, Bovine / Q000235:genetics / Q000276:immunology* / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines