Changes in membrane fatty acids and murein composition of Bacillus cereus and Salmonella Typhi induced by gamma irradiation treatment.
This present study was carried out to evaluate the effects of gamma irradiation on the fatty acids (FA) and mureins composition of two different radiotolerant bacteria. Bacillus cereus LSPQ 2872 and Salmonella Typhi ATCC 19430 were used for this study. The bacterial strains were treated with a sublethal radiation dose of 1kGy to cause a cellular damage. Gas chromatography (GC) and high performance liquid chromatography (HPLC) analysis were performed to demonstrate respectively the modification of the FA composition and the changes in muropeptide profile. Results obtained show, for both bacteria, that this treatment had a significant effect (P< or =0.05) on the FA content with an increase of unsaturated FA percentage. Substantial changes were also noticed for the relative percentage and the number of the muropeptides. This study represents one of the few to demonstrate the modifications on bacterial membrane as a cellular response to survive the ionising radiation stress.
Ayari
Samia
S
National Center for Nuclear Sciences and Technologies, Tunis Cedex, 2020, Tunisia.
Dussault
Dominic
D
Millette
Mathieu
M
Hamdi
Mokhtar
M
Lacroix
Monique
M
D001409:Bacillus cereus / Q000528:radiation effects*; D002462:Cell Membrane / Q000737:chemistry / Q000528:radiation effects*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004307:Dose-Response Relationship, Radiation; D005227:Fatty Acids / Q000032:analysis / Q000528:radiation effects; D005514:Food Irradiation; D005720:Gamma Rays; D010457:Peptidoglycan / Q000032:analysis / Q000528:radiation effects; D012485:Salmonella typhi / Q000528:radiation effects*
D005227:Fatty Acids; D010457:Peptidoglycan