Toxicity of hydrolyzed vicilins toward Callosobruchus maculatus and phytopathogenic fungi.
Studies have shown that vicilins (7S storage proteins) from seeds were able to bind to the surface of the Callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of Diatraea saccharalis and Tenebrio molitor , inhibiting larval development. Vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. The present work studies the association of peptides from vicilins of genotypes of Vigna unguiculata (susceptible and resistant to bruchid) with acetylated chitin and the toxicity of vicilin fragments and chitin-binding vicilin fragments to C. maculatus and phytopathogenic fungi. Hydrolysis of vicilins with alpha-chymotrypsin results in a complex mixture of fragments that were separated by chitin-affinity chromatography. Chitin-binding peptides from both genotypes were toxic to C. maculatus larvae, and alpha-chymotrypsin-hydrolyzed vicilins were deleterious to the above insect and to Fusarium oxysporum , Colletotrichum musae , and Saccharomyces cerevisiae fungi.
Uchôa
Adriana Ferreira
AF
Universidade Estadual do Norte Fluminense, UENF Parque California, Campos dos Goytacazes, RJ, Brazil.
de Miranda
Maria Raquel Alcântara
MR
de Souza
Amanda Jardim
AJ
Gomes
Valdirene Moreira
VM
Fernandes
Kátia Valevski Sales
KV
Lemos
Francisco José Alves
FJ
Oliveira
Antonia Elenir Amancio
AE
Xavier-Filho
José
J
D000818:Animals; D002686:Chitin / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D001517:Coleoptera; D020231:Colletotrichum / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects; D007306:Insecticides; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D055314:Seed Storage Proteins / Q000378:metabolism / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
D005659:Fungicides, Industrial; D007306:Insecticides; D010446:Peptide Fragments; D055314:Seed Storage Proteins; D002686:Chitin; C015575:vicilin protein, plant; D002918:Chymotrypsin; C117608:alpha-chymotrypsin