In vitro response of cutaneous mycosis fungal agents to the most widely used systemic antifungals in dermatology.
BACKGROUND
The high frequency of cutaneous mycosis justify the need to evaluate the possible contribution of in vitro profile of susceptibility to antifungal agents.


OBJECTIVE
To evaluate whether there is variability in in vitro susceptibility by filamentous fungi, previously isolated from cutaneous mycosis, to fluconazole, ketoconazole, itraconazole and terbinafine.


METHODS
Fungi were isolated and identified by classical methods and the antifungal susceptibility test was performed using the method of broth microdilution, according to a protocol recommended by the Clinical Laboratory Standards Institute (CLSI), through M38-A document.


RESULTS
Amongst the 80 filamentous fungi identified, Trichophyton genus represented 81%. The four examined drugs showed great variation for Trichophyton spp and Microsporum spp. Fusarium spp was resistant to all tested drugs. Terbinafine was the most effective drug against the majority of the isolated fungi.


CONCLUSIONS
There was great variability in response profiles to the tested antifungals. The definition of a reference test method will offer higher objectivity for physicians to choose the appropriate therapy.
Almeida
Lívia Maria Martins de
LM
Universidade Estadual de Maringá, Maringá, PR, Brazil.
Souza
Eliane Alves de Freitas
EA
Bianchin
Débora Bertoluzzi
DB
Svidzinski
Terezinha Inez Estivalet
TI
D000935:Antifungal Agents / Q000494:pharmacology*; D003881:Dermatomycoses / Q000382:microbiology; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D003904:Mitosporic Fungi / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D009281:Naphthalenes / Q000494:pharmacology*; D000077291:Terbinafine; D014230:Triazoles / Q000494:pharmacology*
D000935:Antifungal Agents; D009281:Naphthalenes; D014230:Triazoles; D000077291:Terbinafine; D007654:Ketoconazole