title: Differential expression profiling of the synaptosome proteome in a rat model of antipsychotic resistance.
abstract: This study used a comparative proteomics approach to identify the effects of the antipsychotic drugs, Chlorpromazine (CPZ), Clozapine (CLZ), and Quetiapine (QTP) on the synaptosomal protein of the cerebral cortex of Sprague-Dawley (SD) rats. The multivariate statistical test partial least squares-discriminant analysis (PLS-DA) was applied to build the models for screening out the variable important plot (VIP). The PLS-DA models were able to distinguish each drug treatment group and the control group; more importantly, the univariate differentially expressed protein spots were capable of being verified by the VIP of the models. The interrelationships among the identified proteins were analyzed using Pearson's correlation analysis and pathway analysis. Through the synaptosome proteome experiments, we established that the energy production of the mitochondrial function and 'Glycolysis/Gluconeogenesis' were involved in the response to antipsychotic medications. Furthermore, the G protein-coupled signal transduction system was also inhibited by antipsychotic medications. The result of our study should contribute to the understanding of the effects of antipsychotic drugs on synaptic function.
authors: Bio-X Center, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
authors: Baohu
authors: B
authors: Ji
authors: Zhao
authors: Z
authors: Zhang
authors: Ming
authors: M
authors: Zhang
authors: Hui
authors: H
authors: Zhu
authors: Kejun
authors: K
authors: Zhou
authors: Jinglei
authors: J
authors: Yang
authors: Yang
authors: Y
authors: Li
authors: Liya
authors: L
authors: Sun
authors: Guoyin
authors: G
authors: Feng
authors: Yujiong
authors: Y
authors: Wang
authors: Lin
authors: L
authors: He
authors: Chunling
authors: C
authors: Wan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000502:physiology; D002746:Chlorpromazine / Q000494:pharmacology; D003024:Clozapine / Q000494:pharmacology; D003987:Dibenzothiazepines / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D013058:Mass Spectrometry; D015999:Multivariate Analysis; D010449:Peptide Mapping; D020543:Proteome / Q000187:drug effects* / Q000502:physiology; D040901:Proteomics; D000069348:Quetiapine Fumarate; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013574:Synaptosomes / Q000187:drug effects* / Q000502:physiology
chemical_list: D014150:Antipsychotic Agents; D003987:Dibenzothiazepines; D020543:Proteome; D000069348:Quetiapine Fumarate; D003024:Clozapine; D002746:Chlorpromazine