[Two-dimensional liquid phase chromatographic proteome profiles in Uighur women with uterine cervix cancer].
OBJECTIVE
To establish differential protein expression profile specific for serum proteome of cervical carcinoma in Uighur women by protein 2-dimensional liquid phase chromatography.


METHODS
We collected and prepared sera of cervical carcinoma from Uighur women, and separated and processed data using 2-dimensional liquid phase chromatography system specific for protein (ProteomeLab PF-2D).


RESULTS
A differential expression profile of serum proteome based on characteristics of protein isoelectric point and hydrophobic features was successfully established. Fifty-six differentially expressed protein spots were found, among which there was an obvious difference in 12 peaks.


CONCLUSIONS
Two-dimensional liquid phase chromatography is an effective and feasible method to establish differential expression profile of serum proteome and to offer a new way for the screening of serum protein markers of Uighur women with cervical carcinoma.
Guo
Xia
X
Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases, Xinjiang Medical University, Urumqi 830011, China.
Abulizi
Abudula
A
Wu
Guizhen
G
Liu
Kaijiang
K
D014408:Biomarkers, Tumor / Q000097:blood; D002681:China / Q000208:ethnology; D002853:Chromatography, Liquid / Q000379:methods*; D005006:Ethnicity; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D009363:Neoplasm Proteins / Q000097:blood*; D020543:Proteome / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism*
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020543:Proteome