Mechanotransductive surfaces for reversible biocatalysis activation.
Fibronectin, like other proteins involved in mechanotransduction, has the ability to exhibit recognition sites under mechanical stretch. Such cryptic sites are buried inside the protein structure in the native fold and become exposed under an applied force, thereby activating specific signalling pathways. Here, we report the design of new active polymeric nanoassembled surfaces that show some similarities to these cryptic sites. These nanoassemblies consist of a first polyelectrolyte multilayer stratum loaded with enzymes and capped with a second polyelectrolyte multilayer acting as a mechanically sensitive nanobarrier. The biocatalytic activity of the film is switched on/off reversibly by mechanical stretching, which exposes enzymes through the capping barrier, similarly to mechanisms involved in proteins during mechanotransduction. This first example of a new class of biologically inspired surfaces should have great potential in the design of various devices aimed to trigger and modulate chemical reactions by mechanical action with applications in the field of microfluidic devices or mechanically controlled biopatches for example.
Mertz
Damien
D
Institut National de la Santé et de la Recherche Médicale, INSERM Unité 977, Biomaterials and Tissue Engineering, 11 rue Humann, 67085 Strasbourg Cedex, France.
Vogt
Cédric
C
Hemmerlé
Joseph
J
Mutterer
Jérôme
J
Ball
Vincent
V
Voegel
Jean-Claude
JC
Schaaf
Pierre
P
Lavalle
Philippe
P
D000327:Adsorption; D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D001696:Biomechanical Phenomena; D011756:Diphosphates / Q000737:chemistry; D004573:Electrolytes; D004789:Enzyme Activation; D019793:Fluorescein / Q000737:chemistry / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry; D010084:Oxidation-Reduction; D011107:Polylysine / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties
D011756:Diphosphates; D004573:Electrolytes; D011107:Polylysine; D006820:Hyaluronic Acid; D000469:Alkaline Phosphatase; D019793:Fluorescein