Azide solid support for 3'-conjugation of oligonucleotides and their circularization by click chemistry.
A solid support bearing an azido linker was used to synthesize a 3'-azido-alkyl-oligonucleotide by phosphoramidite chemistry. The resulting oligonucleotide was either conjugated by 1,3-dipolar cycloaddition on solid support or in solution with mannose-propargyl derivative and in solution with dansyl propargyl. Besides, after introduction of an alkyne function at the 5'-end, the resulting oligonucleotide bearing both 3'-azide and 5'-alkyne functions was circularized.
Pourceau
Gwladys
G
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université Montpellier 1, Université Montpellier 2, Place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
Meyer
Albert
A
Vasseur
Jean-Jacques
JJ
Morvan
François
F
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry*; D002625:Chemistry, Organic / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D008956:Models, Chemical; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
D000480:Alkynes; D001386:Azides; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA; D008358:Mannose