Observation of cellular cholesterol efflux at microcavity electrodes.
Cholesterol oxidase modified platinum microcavity electrodes are used to measure cholesterol efflux from the plasma membrane surface of a single neuron in the buccal ganglion of Aplysia at room temperature. A background subtraction analog chronocoulometry method is used to measure hydrogen peroxide accumulation in the microcavity volume resulting from cellular cholesterol efflux and enzymatic oxidation. The data are consistent with the aqueous diffusion model for cellular cholesterol efflux where plasma membrane cholesterol undergoes exchange with solution phase cholesterol.
Fang
Danjun
D
Department of Chemistry, Case Western Reserve University and Rainbow Babies and Children's Hospital, Cleveland, Ohio 44106, USA.
Jiang
Dechen
D
Lu
Hui
H
Chiel
Hillel J
HJ
Kelley
Thomas J
TJ
Burgess
James D
JD
D000818:Animals; D001048:Aplysia; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D005724:Ganglia / Q000166:cytology; D008839:Microelectrodes; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*
D002784:Cholesterol