High consistency enzymatic hydrolysis of hardwood substrates.
The feasibility of using a laboratory peg mixer to carry out high consistency enzymatic hydrolysis of lignocellulosic substrates was investigated. Two hardwood substrates, unbleached hardwood pulp (UBHW) and organosolv pretreated poplar (OPP), were used in this study. Hydrolysis of UBHW and OPP at 20% substrate consistency led to a high glucose concentration in the final hydrolysate. For example, a 48 h enzymatic hydrolysis of OPP resulted in a hydrolysate with 158 g/L of glucose. This is the highest glucose concentration ever obtained from enzymatic hydrolysis of lignocellulosic substrates. Fermentation of UBHW and OPP hydrolysates with high glucose content led to high ethanol concentrations, 50.4 and 63.1 g/L, respectively after fermentation. Our results demonstrate that using common pulping equipment to carry out high consistency hydrolysis can overcome the rheological problems and greatly increase the sugar and ethanol concentrations after the hydrolysis and fermentation.
Zhang
Xiao
X
FPInnovations Paprican Division (PAPRICAN), 570 boul. St-Jean, Pointe-Claire, Quebec, Canada H9R 3J9. xiao.zhang@fpinnovations.ca
Qin
Wenjuan
W
Paice
Michael G
MG
Saddler
John N
JN
D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D004867:Equipment Design; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D032107:Populus; D012212:Rheology; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013997:Time Factors
D002241:Carbohydrates; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D005947:Glucose