Critical role of ESCRT machinery in EGFR recycling.
The molecular mechanisms of EGFR vesicular trafficking to lysosomes have recently received considerable attention. It is now clear that endosomal sorting complexes required for transport (ESCRTs) are critical for EGFR degradation. Although an increasing number of membrane receptors also undergo recycling via specific pathways, little information is available regarding regulated recycling of EGFR. In this study, we investigated the roles of ESCRTs in EGFR recycling after stimulation with amphiregulin (AR). We used ESCRT small interfering RNA (siRNA) duplexes to demonstrate that AR-induced EGFR intracellular processing involves active sorting to the recycling pathway through specific members of the ESCRT family.
Baldys
Aleksander
A
Nephrology Division, Department of Medicine, Medical University of South Carolina, and Medical and Research Services, Ralph H Johnson Veterans Affairs Medical Center, Charleston, South Carolina 29425, USA.
Raymond
John R
JR
D066258:Amphiregulin; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors; D066255:EGF Family of Proteins; D004705:Endocytosis / Q000502:physiology; D056827:Endosomal Sorting Complexes Required for Transport / Q000037:antagonists & inhibitors; D011992:Endosomes / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D066246:ErbB Receptors / Q000737:chemistry* / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000502:physiology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000502:physiology; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry / Q000502:physiology; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D010750:Phosphoproteins / Q000037:antagonists & inhibitors; D021381:Protein Transport / Q000502:physiology; D050721:Proto-Oncogene Proteins c-cbl / Q000037:antagonists & inhibitors / Q000737:chemistry; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000037:antagonists & inhibitors; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D000099177:Tsg101 Protein
D066258:Amphiregulin; D004268:DNA-Binding Proteins; D066255:EGF Family of Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D066246:ErbB Receptors; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D010750:Phosphoproteins; D050721:Proto-Oncogene Proteins c-cbl; D020794:Receptor Protein-Tyrosine Kinases; D014157:Transcription Factors; D025801:Ubiquitin; D033921:Vesicular Transport Proteins; D000099177:Tsg101 Protein; C587848:AREG protein, human; C498543:CHMP3 protein, human; C544455:Vps25 protein, human; C096164:hepatocyte growth factor-regulated tyrosine kinase substrate