Characterization of Lactobacillus spp. isolated from the feces of breast-feeding piglets.
Lactobacillus spp., referred to as IJ-1 and IJ-2, were isolated from the feces of breast-feeding piglets and analyzed for probiotic properties. According to the analyses of 16S rDNA sequence, Lactobacillus sp. IJ-1 showed greater than 99% homology with Lactobacillus reuteri DSM 20016(T), and Lactobacillus sp. IJ-2 had greater than 99% homology with the L. gasseri ATCC 33323(T) and L. johnsonii ATCC 33200(T). The pH changes in the culture media of Lactobacillus sp. IJ-1 and Lactobacillus sp. IJ-2 were from 6.5 to 4.2 and 4.6, respectively. Their respective resistance against artificial gastric acid and artificial bile acid led to survival rates of nearly 186+/-44% and 13+/-5%. Neither strain produced the carcinogenic enzyme beta-glucuronidase. Both strains inhibited the growth of pathogenic microorganisms, such as Listeria monocytogenes ATCC 19111, Salmonella enterica KCTC 12401, Salmonella enteritidis ATCC 13076, Staphylococcus aureus KCTC 3881, and Bacillus cereus 3711, within 24 h of growth.
Cho
Il Jae
IJ
Department of Biotechnology, Chung-Ang University, Anseong 456-756, South Korea.
Lee
Nam Keun
NK
Hahm
Young Tae
YT
D000818:Animals; D000831:Animals, Newborn; D000890:Anti-Infective Agents / Q000494:pharmacology; D001409:Bacillus cereus / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005243:Feces; D005744:Gastric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D019779:Salmonella enterica / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism; D013552:Swine
D000890:Anti-Infective Agents; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S