title: Identification of the lipid mediator prostaglandin E2 in tissue immune cells of humans infected with the filaria Onchocerca volvulus.
abstract: Prostaglandins generated by multiple tissue and immune cells exhibit regulatory effects on the vascular and immune systems. Prostaglandin E(2) (PGE(2)), in particular, affects innate as well as adaptive immune mechanisms. We identified PGE(2) in host immune cells adjacent to Onchocerca volvulus in subcutaneous onchocercomas and the affected skin. Using immunohistology, PGE(2) was predominantly detected in infiltrating macrophages but also in plasma cells. Consecutive sections revealed concomitant presence of PGE(2) and transforming growth factor-beta (TGF-beta), representing a second immunoregulative mediator in macrophages and plasma cells. TGF-beta was preferentially observed in the infiltrating macrophages in patients with a generalized hyporeactive onchocerciasis and less in patients with the hyperreactive form. The presence of PGE(2) and TGF-beta in adjoining host cells infiltrating in the onchocercoma and dermis may indicate containment of inflammatory responses that could favour survival of the filarial parasite.
authors: Bernhard Nocht Institute for Tropical Medicine, D-20359 Hamburg, Germany. nbrattig@bni-hamburg.de
authors: Norbert W
authors: NW
authors: Brattig
authors: Arline
authors: A
authors: Schwohl
authors: Achim
authors: A
authors: Hoerauf
authors: Dietrich W
authors: DW
authors: Büttner
mesh_terms: D000818:Animals; D015232:Dinoprostone / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000737:chemistry*; D008297:Male; D017181:Onchocerca volvulus / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology* / Q000473:pathology; D010950:Plasma Cells / Q000737:chemistry*; D012867:Skin / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000032:analysis
chemical_list: D016212:Transforming Growth Factor beta; D015232:Dinoprostone