Optical detection of specific genes for genetically modified soybean and maize using multiplex PCR coupled with primer extension on a plastic plate.
A novel DNA microarray method to detect one line of genetically modified (GM) soybean and five lines of GM maize was developed using multiplex PCR coupled with primer extension on a plastic plate. Multiplex PCR products were applied on an extension primer-immobilized plate and the spots corresponding to the DNA sequences were visualized. This method is a rapid and simple way to detect GM soybean and GM maize optically.
Harikai
Naoki
N
School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan. harikai@mukogawa-u.ac.jp
Saito
Shin
S
Abe
Midori
M
Kondo
Kazunari
K
Kitta
Kazumi
K
Akiyama
Hiroshi
H
Teshima
Reiko
R
Kinoshita
Kenji
K
D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D030841:Food, Genetically Modified; D017343:Genes, Plant / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D055620:Optical Phenomena; D030821:Plants, Genetically Modified; D010969:Plastics; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics*; D013997:Time Factors; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D010969:Plastics