[Effects of fluid shear stress time on carbonic anhydrase in rat osteoclasts].
OBJECTIVE
To observe the effects of fluid shear stress on carbonic anhydrase II in rat osteoclasts.


METHODS
Osteoclasts were subject to fluid shear stress for 0,15,30,60 and 120 minutes respectively, which was 2.9dyne/cm(2). mRNA level of carbonic anhydrase II was detected through real-time fluorescent quantitation PCR. The data were analyzed with SPSS 12.0 software package.


RESULTS
mRNA level of carbonic anhydrase II in control group, 15 minutes,30 minutes,60 minutes and 120 minutes group was 7.88+/-0.09, 11.14+/-0.12, 15.83+/-0.18, 1.94+/-0.02, and 1.37+/-0.01(E+5 copy number), respectively. There was significant difference between any two groups (P<0.05).


CONCLUSIONS
In this present study, mRNA level of carbonic anhydrase II has an increasing tendency with various action times of fluid shear stress, it reaches to a peak value at 30 minutes. We speculated that there might be a certain time-effect relation between fluid shear stress and mRNA level of carbonic anhydrase II, but this mechanism need be further studied.
Zhu
Bao-min
BM
Department of Prosthetics, Hangzhou Stomatological Hospital, Hangzhou 310006, China.
Zhang
Qing-hong
QH
Wang
Wei-qian
WQ
D000818:Animals; D001729:Bismuth; D002256:Carbonic Anhydrases; D002478:Cells, Cultured; D009942:Organometallic Compounds; D010010:Osteoclasts; D012333:RNA, Messenger; D051381:Rats; D012459:Salicylates; D013314:Stress, Mechanical
D009942:Organometallic Compounds; D012333:RNA, Messenger; D012459:Salicylates; C015715:bismuth subsalicylate; D002256:Carbonic Anhydrases; D001729:Bismuth