Isolating a cytoprotective compound from Ganoderma tsugae: effects on induction of Nrf-2-related genes in endothelial cells.
Ganoderma tsugae is a medicinal fungus with several biological activities. It has long been used as a folk remedy for the promotion of health and longevity in China and other oriental countries. Here, a bioactive fraction of G. tsugae was progressively purified to be enriched in the activity of cytoprotective enzymes. The highest bioactivity was detected in the 20% EtOH-precipitated fraction, which was prepared from submerged fermentation filtrate of G. tsugae. Following further purification by gel filtration chromatography and acetone extraction, the most bioactive fraction, F5-2, was identified as a peptidoglycan-like compound. Extracts of G. tsugae (F5-2) induced heme oxygenase-1 (HO-1) and thioredoxin reductase-1 (TrxR1) expression in endothelial cells by increasing NF-E2-related factor-2 (Nrf2) nuclear translocation. Pretreatment with F5-2 increased intracellular glutathione (GSH) and protected against H(2)O(2), suggesting that induction of these antioxidant enzymes is important in protection against oxidative stress. Hence the bioactive peptidoglycan-like compound from G. tsugae might protect endothelial cells.
Wei
Yu-Shan
YS
Institute of Biotechnology, National Chiayi University, Taiwan.
Wung
Being-Sun
BS
Lin
Yuan-Chun
YC
Hsieh
Chia-Wen
CW
D000818:Animals; D001681:Biological Assay; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology; D002417:Cattle; D019610:Cytoprotection; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D032902:Ganoderma / Q000737:chemistry*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D017930:Genes, Reporter; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051267:NF-E2-Related Factor 2 / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects; D010457:Peptidoglycan / Q000302:isolation & purification* / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects; D013879:Thioredoxins / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects*
D002316:Cardiotonic Agents; D006023:Glycoproteins; D051267:NF-E2-Related Factor 2; D010457:Peptidoglycan; D013879:Thioredoxins; D008156:Luciferases; D051547:Heme Oxygenase-1