title: In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror.
abstract: We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice.
authors: James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, Stanford, California 94305, USA.
authors: Wibool
authors: W
authors: Piyawattanametha
authors: Eric D
authors: ED
authors: Cocker
authors: Laurie D
authors: LD
authors: Burns
authors: Robert P
authors: RP
authors: Barretto
authors: Juergen C
authors: JC
authors: Jung
authors: Hyejun
authors: H
authors: Ra
authors: Olav
authors: O
authors: Solgaard
authors: Mark J
authors: MJ
authors: Schnitzer
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000166:cytology*; D002196:Capillaries / Q000166:cytology*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007909:Lenses; D051379:Mice; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity