title: Deep tissue multiphoton microscopy using longer wavelength excitation.
abstract: We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain. We achieved high contrast imaging of blood vessels at approximately twice the depth with 1280-nm excitation as with 775-nm excitation. An imaging depth of 1 mm can be achieved in in vivo imaging of adult mouse brains at 1280 nm with approximately 1-nJ pulse energy at the sample surface. Blood flow speed measurements at a depth of 900 mum are performed.
authors: School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA. dk287@cornell.edu
authors: Demirhan
authors: D
authors: Kobat
authors: Michael E
authors: ME
authors: Durst
authors: Nozomi
authors: N
authors: Nishimura
authors: Angela W
authors: AW
authors: Wong
authors: Chris B
authors: CB
authors: Schaffer
authors: Chris
authors: C
authors: Xu
mesh_terms: D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008027:Light; D008029:Lighting / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D008962:Models, Theoretical; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity