title: Effects of cysteine during in vitro maturation of porcine oocytes under low oxygen tension on their subsequent in vitro fertilization and development.
abstract: In this study, we evaluated the effect of different concentrations of cysteine in in vitro maturation (IVM) medium during IVM under low oxygen tension (5% O(2)) of porcine oocytes on the intracellular content of glutathione (GSH) and subsequent in vitro fertilization (IVF) and development. Cumulus oocyte complexes (COCs) were collected from ovaries obtained at a local slaughterhouse, cultured in IVM medium supplemented with 0 (control), 0.05, 0.1, 0.2 or 0.6 mM cysteine for 44-46 h, fertilized in vitro and subsequently cultured for 6 days in total. The GSH content of the IVM oocytes exposed to 0, 0.05, 0.1, 0.2 or 0.6 mM cysteine increased significantly (P<0.05) as the concentration of cysteine increased (12.2, 14.0, 15.1, 16.4 and 16.4 pmol/oocyte, respectively). However, the rates of oocyte maturation, sperm penetration, male pronuclear formation, monospermy and even cleavage on Day 2 (the day of IVF was defined as Day 0) and blastocyst formation on Day 6 did not differ among the groups. Moreover, the cell numbers of blastomeres in blastocysts were uniform among the groups. These results indicate that supplementation with 0.05-0.6 mM cysteine during IVM under 5% O(2) tension significantly increased the intracellular GSH contents of IVM oocytes; however, it had no promoting effects on nuclear maturation, fertilization, male pronucleus formation and subsequent embryonic development to the blastocyst stage.
authors: National Institute of Livestock and Grassland Science, Ibaraki, Japan.
authors: Nguyen
authors: N
authors: Viet Linh
authors: Thanh Quang
authors: TQ
authors: Dang-Nguyen
authors: Bui Xuan
authors: BX
authors: Nguyen
authors: Noboru
authors: N
authors: Manabe
authors: Takashi
authors: T
authors: Nagai
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D003545:Cysteine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008623:Mercaptoethanol; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013552:Swine; D015053:Zygote / Q000187:drug effects
chemical_list: D000975:Antioxidants; D008623:Mercaptoethanol; D005978:Glutathione; D003545:Cysteine