title: Common crowding agents have only a small effect on protein-protein interactions.
abstract: Studies of protein-protein interactions, carried out in polymer solutions, are designed to mimic the crowded environment inside living cells. It was shown that crowding enhances oligomerization and polymerization of macromolecules. Conversely, we have shown that crowding has only a small effect on the rate of association of protein complexes. Here, we investigated the equilibrium effects of crowding on protein heterodimerization of TEM1-beta-lactamase with beta-lactamase inhibitor protein (BLIP) and barnase with barstar. We also contrasted these with the effect of crowding on the weak binding pair CyPet-YPet. We measured the association and dissociation rates as well as the affinities and thermodynamic parameters of these interactions in polyethylene glycol and dextran solutions. For TEM1-BLIP and for barnase-barstar, only a minor reduction in association rate constants compared to that expected based on solution viscosity was found. Dissociation rate constants showed similar levels of reduction. Overall, this resulted in a binding affinity that is quite similar to that in aqueous solutions. On the other hand, for the CyPet-YPet pair, aggregation, and not enhanced dimerization, was detected in polyethylene glycol solutions. The results suggest that typical crowding agents have only a small effect on specific protein-protein dimerization reactions. Although crowding in the cell results from proteins and other macromolecules, one may still speculate that binding in vivo is not very different from that measured in dilute solutions.
authors: Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
authors: Yael
authors: Y
authors: Phillip
authors: Eilon
authors: E
authors: Sherman
authors: Gilad
authors: G
authors: Haran
authors: Gideon
authors: G
authors: Schreiber
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D002151:Calorimetry; D003911:Dextrans / Q000737:chemistry; D019855:Ethylene Glycol / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008027:Light; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D055503:Protein Multimerization; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance; D014783:Viscosity; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003911:Dextrans; D011108:Polymers; D065093:beta-Lactamase Inhibitors; C057438:barstar protein, Bacillus amyloliquefaciens; D011092:Polyethylene Glycols; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease; C121000:TEM24 beta-lactamase; D001618:beta-Lactamases; D019855:Ethylene Glycol; D005947:Glucose