Bioassay-guided isolation of an anti-ulcer chromene from Eupatorium aschenbornianum: role of nitric oxide, prostaglandins and sulfydryls.
Eupatorium aschenbornianum is considered useful in the treatment of gastric ulcer. In the current study the validity of this practice was tested by using the experimental model of an ethanol induced gastric ulcer in rats. The results show that E. aschenbornianum had gastroprotective activity, that the hexane extract had the highest protective activity (85.65+/-4.76%), and that encecanescin isolated from this extract was the main active gastroprotective agent. The effect elicited by encecanescin was attenuated by N(G)-nitro-L-arginine methyl ester, N-ethylmaleimide and indomethacin, which suggests that NO, prostaglandins and sulfydryl groups are involved in the mechanisms of gastroprotective action.
Sánchez-Mendoza
María Elena
ME
Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Colonia Santo Tomás, Delegación Miguel Hidalgo 11340, México DF, México.
Reyes-Trejo
Benito
B
Sánchez-Gómez
Paula
P
Rodríguez-Silverio
Juan
J
Castillo-Henkel
Carlos
C
Cervantes-Cuevas
Humberto
H
Arrieta
Jesús
J
D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D001578:Benzopyrans / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D002229:Carbenoxolone / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical; D000431:Ethanol; D005033:Ethylmaleimide; D036602:Eupatorium / Q000737:chemistry*; D007213:Indomethacin; D008297:Male; D008519:Medicine, Traditional; D015394:Molecular Structure; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D013438:Sulfhydryl Compounds / Q000037:antagonists & inhibitors
D000897:Anti-Ulcer Agents; D001578:Benzopyrans; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D000431:Ethanol; C066390:encecanescin; D002229:Carbenoxolone; D005033:Ethylmaleimide; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin