title: Biochemical and molecular characterization of some Centaurea species growing in the eastern Anatolia region of Turkey.
abstract: Random amplified polymorphic DNA (RAPD) and fatty acid (FAME) profiles were used to examine phenotypic and genetic relationships among 16 Centaurea species growing wild in the eastern Anatolia region of Turkey. Thirteen decamer primers were used to examine polymorphism. According to the RAPD results, 99 amplicons in the size range of 50-1000 bp were produced from 13 primers in 16 Centaurea species. Genetically four distinct groups were determined among the species of Centaurea, which represents high genetic variation. In the 16 species, 14 fatty acids were determined according to FAME results. Both FAME and RAPD results showed that C. virgata is genetically different from other species. The differences in the composition of fatty acids among Centaurea species suggest that fatty acid profiles could be used to differentiate among some of these species. Results of this study show that RAPD and FAME analyses are consistent.
authors: Faculty of Art and Science, Department of Biology, Atatürk University, Erzurum, Turkey.
authors: Nalan
authors: N
authors: Yildirim
authors: Serap
authors: S
authors: Sunar
authors: Guleray
authors: G
authors: Agar
authors: Sedat
authors: S
authors: Bozari
authors: Ozkan
authors: O
authors: Aksakal
mesh_terms: D031179:Centaurea / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics; D008738:Methyl Ethers / Q000032:analysis; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014421:Turkey
chemical_list: D005227:Fatty Acids; D005819:Genetic Markers; D008738:Methyl Ethers