Molecular immune signatures of HIV-1 vaccines in human PBMCs.
The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.
Monaco
Alessandro
A
Infectious Disease and Immunogenetics Section, Department of Transfusion Medicine, Clinical Center, National Institutes of Health Bethesda, MD, USA.
Marincola
Francesco M
FM
Sabatino
Marianna
M
Pos
Zoltan
Z
Tornesello
Maria Lina
ML
Stroncek
David F
DF
Wang
Ena
E
Lewis
George K
GK
Buonaguro
Franco M
FM
Buonaguro
Luigi
L
D016915:AIDS Vaccines / Q000494:pharmacology*; D016022:Case-Control Studies; D018925:Chemokines / Q000096:biosynthesis; D020869:Gene Expression Profiling / Q000379:methods*; D015658:HIV Infections; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D019562:Viral Load
D016915:AIDS Vaccines; D018925:Chemokines