Post-derivatization procedure for determination of hippuric acid after extraction by an automated micro solid phase extraction system and monitoring by gas chromatography.
A rapid, simple and high sensitive method is described here for extraction of hippuric acid (HA) from human urine samples by using an automated micro solid phase extraction system (mu-SPE). However in order to increase sensitivity of gas chromatography with flame ionization detector, a post derivatization procedure was developed. In this work, a polypyrrole was synthesized by chemical oxidation of the pyrrole monomer in non-aqueous solution and applied as an excellent and efficient sorbent for mu-SPE. The calibration curves were linear in the range of 0.018-8.95microg mL(-1) for HA, in both water and urine samples with correlation coefficients 0.9973 and 0.9946, respectively; limits of detections were 12.1ngmL(-1) and 16.5ngmL(-1), respectively. This method was successfully used to analyze trace amounts of HA in human urine samples without any interference from coexisting substances.
Ahmadi
F
F
Department of Medicinal Chemistry, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67145-1673, IR Iran. fahmadi@kums.ac.ir
Asgharloo
H
H
Sadeghi
S
S
Gharehbagh-Aghababa
V
V
Adibi
H
H
D002849:Chromatography, Gas / Q000379:methods*; D004867:Equipment Design; D006626:Hippurates / Q000652:urine*; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*
D006626:Hippurates; D012965:Sodium Chloride; D006851:Hydrochloric Acid; C030514:hippuric acid; D000432:Methanol