Identification of seven metabolites of oxyresveratrol in rat urine and bile using liquid chromatography/tandem mass spectrometry.
Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene) is a major compound isolated from Smilax china, a Chinese herbal medicine. The rat urine and bile samples were pretreated by solid-phase extraction method after oral administration at a dose of 100 mg/kg of oxyresveratrol. Seven metabolites were identified by LC-MS/MS method with electrospray ionization in negative ion mode. The results indicated that main metabolites of oxyresveratrol were monoglucuronided and monosulfated oxyresveratrol. Based on the results, the metabolic pathway of oxyresveratrol in rat urine and bile was proposed.
Huang
Huilian
H
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 199 Guo Shoujing Road, Zhangjiang, Shanghai, People's Republic of China.
Chen
Guangtong
G
Lu
Zhiqiang
Z
Zhang
Jinqiang
J
Guo
De-An
DA
D000818:Animals; D001646:Bile / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D008297:Male; D053858:Metabolic Networks and Pathways; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D032281:Smilax / Q000737:chemistry; D052616:Solid Phase Extraction; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad