The fungal glutathione S-transferase system. Evidence of new classes in the wood-degrading basidiomycete Phanerochaete chrysosporium.
The recent release of several basidiomycete genome sequences allows an improvement of the classification of fungal glutathione S-transferases (GSTs). GSTs are well-known detoxification enzymes which can catalyze the conjugation of glutathione to non-polar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. Following this mechanism, they are able to metabolize drugs, pesticides, and many other xenobiotics and peroxides. A genomic and phylogenetic analysis of GST classes in various sequenced fungi--zygomycetes, ascomycetes, and basidiomycetes--revealed some particularities in GST distribution, in comparison with previous analyses with ascomycetes only. By focusing essentially on the wood-degrading basidiomycete Phanerochaete chrysosporium, this analysis highlighted a new fungal GST class named GTE, which is related to bacterial etherases, and two new subclasses of the omega class GSTs. Moreover, our phylogenetic analysis suggests a relationship between the saprophytic behavior of some fungi and the number and distribution of some GST isoforms within specific classes.
Morel
Mélanie
M
IFR 110 Ecosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation, Unité Mixte de Recherches INRA UHP 1136 Interaction Arbres Microorganismes, Université Nancy I BP 239, 54506 Vandoeuvre-lès-Nancy Cedex, France. mmorel@scbiol.uhp-nancy.fr
Ngadin
Andrew A
AA
Droux
Michel
M
Jacquot
Jean-Pierre
JP
Gelhaye
Eric
E
D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000201:enzymology*; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014934:Wood / Q000378:metabolism*
D005656:Fungal Proteins; D020033:Protein Isoforms; D005982:Glutathione Transferase