Mid-infrared absorption spectroscopy and differential damage in vitro between lipids and proteins by an all-fiber-integrated supercontinuum laser.
We identify and differentially damage lipids and proteins using wavelengths between 2.6 and 3.8 mum from a fiber-based supercontinuum (SC) laser. Absorption spectroscopy of the constituents of normal artery and atherosclerotic plaque, including adipose tissue, macrophages and foam cells, are measured by a SC laser in the mid-infrared. By using the laser light within the C-H fatty acid and cholesterol esters absorption band, we also demonstrate differential damage of lipid-rich adipose tissue without damaging the protein-rich blood vessel wall. The experiments use a novel SC laser that is all-fiber-integrated with no moving parts, covers a continuous spectrum ranging from approximately 0.8 to beyond 4.2 microm, and outputs a time-averaged power scalable up to 10.5 W.
Ke
Kevin
K
Department of Electrical Engineering and Computer Science, University of Michigan, 1301 Beal Avenue, Ann Arbor, Michigan 48109, USA.
Xia
Chenan
C
Islam
Mohammed N
MN
Welsh
Michael J
MJ
Freeman
Michael J
MJ
D000273:Adipose Tissue / Q000473:pathology; D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D002788:Cholesterol Esters / Q000737:chemistry; D004867:Equipment Design; D005227:Fatty Acids / Q000737:chemistry; D005487:Foam Cells / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D008055:Lipids / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D051379:Mice; D011506:Proteins / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013997:Time Factors
D002788:Cholesterol Esters; D005227:Fatty Acids; D008055:Lipids; D011506:Proteins