title: [Relationship between p53 gene and chromosome 13q14 variations and prognosis in primary intestinal lymphoma].
abstract: OBJECTIVE
  Some research has shown that primary intestinal lymphoma with the same immunophenotype has different prognosis. It suggests that the prognosis of this disease is not determined by a single factor but may be related to genetic or chromosomal variations. The p53 gene is an important tumor suppressor gene, and 13q14 deletion is a common chromosomal abnormality of lymphocyte proliferation diseases. This study aimed to explore the role of the p53 gene and chromosome 13q14 variations in the assessment of prognosis in primary intestinal lymphoma.
  
  
  METHODS
  p53 gene and chromosome 13q14 expression in paraffin sections of 30 cases of primary intestinal lymphoma and 10 cases of lymph node reactive hyperplasia were ascertained using an improved FISH technique.
  
  
  RESULTS
  p53 gene deletion was found in 22.7% of patients with primary intestinal lymphoma at stage I-II and in 75.0% of patients at stage III-IV (x2=6.903, p<0.01). The 30 patients with primary intestinal lymphoma were pathologically classified into-mucosa-associated lymphoid tissue (MALT) (n=14) and non-MALT types (n=16). The MALT lymphoma group had significantly lower incidence of p53 gene deletion (14.3% vs 56.3%; x2=5.662, p<0.05). Average survival time in patients with p53 gene deletion was 13.41 months, being shorter than the patients with normal p53 gene (36.1 months) (t=2.637, p<0.05). 13q14 deletion was found in 40.0% of patients with primary intestinal lymphoma, but none of patients with lymph node reactive hyperplasia showed 13q14 deletion. 13q14 deletion was not significantly related to the pathological type and the clinical stage of primary intestinal lymphoma as well as the survival time. There was no significant correlation between p53 gene and 13q14 deletions.
  
  
  CONCLUSIONS
  There was a high incidence of p53 gene deletion in patients with non-MALT lymphoma or at stage III-IV. p53 gene deletion is related to a high tumor malignant degree and a poor prognosis, while-chromosome 13q14 variation is not associated with the prognosis in patients with primary intestinal lymphoma.
authors: Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, China. fengljz@sina.com
authors: Li-Juan
authors: LJ
authors: Feng
authors: Guo-Ping
authors: GP
authors: Zhang
authors: Min
authors: M
authors: Xie
authors: Peng-Fei
authors: PF
authors: Cao
authors: Chun-Yan
authors: CY
authors: Fu
authors: Zhong-Liang
authors: ZL
authors: Hu
authors: Mao
authors: M
authors: Dai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002869:Chromosome Aberrations; D002882:Chromosomes, Human, Pair 13; D016158:Genes, p53; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007414:Intestinal Neoplasms / Q000235:genetics* / Q000401:mortality; D008223:Lymphoma / Q000235:genetics* / Q000401:mortality; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics / Q000401:mortality; D008875:Middle Aged; D011379:Prognosis