title: Magnetic bead-based detection of autoimmune responses using protein microarrays.
abstract: In the present study, a magnetic bead-based detection approach for protein microarrays is described as an alternative approach to the commonly used fluorescence-based detection system. Using the bead-based detection approach with applied magnetic force, it was possible to perform the detection step more rapidly as a result of the accelerated binding between the captured analyte in the microspot and the detection antibody, which was coupled to the magnetic beads. The resulting strong opacity shift on the microspots could be recorded with an ordinary flatbed scanner. In the context of autoimmunity, a set of 24 serum samples was analyzed for the presence of antibodies against 12 autoantigens using standard fluorescence and magnetic bead-based detection methods. Dynamic range, sensitivity, and specificity were determined for both detection methods. We propose from our findings that the magnetic bead-based detection option provides a simplified and cost effective readout method for protein microarrays.
authors: Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
authors: Jesper
authors: J
authors: Gantelius
authors: Michael
authors: M
authors: Hartmann
authors: Jochen M
authors: JM
authors: Schwenk
authors: Johan
authors: J
authors: Roeraade
authors: Helene
authors: H
authors: Andersson-Svahn
authors: Thomas O
authors: TO
authors: Joos
mesh_terms: D015551:Autoimmunity / Q000276:immunology*; D016000:Cluster Analysis; D005453:Fluorescence; D006801:Humans; D008280:Magnetics / Q000379:methods*; D008863:Microspheres; D040081:Protein Array Analysis / Q000379:methods* / Q000592:standards; D012372:ROC Curve; D015203:Reproducibility of Results