In vivo histocompatibility evaluation of polyurethane membrane modified by superfine silk-fibroin powder.
In this study, a novel polyurethane membrane, modified by superfine silk-fibroin powder, was prepared for small-diameter vascular grafting. Scanning electron microscopy, transmission electron microscopy, and histological examination were applied to evaluate histocompatibility of this polyurethane membrane. The polyurethane membrane was compared with polytetrafluoroethylene material. A pseudomembrane and gap formed between polytetrafluoroethylene and the surrounding tissues, and no cells infiltrated or grew into the polytetrafluoroethylene material. On the contrary, superfine silk-fibroin powder/polyurethane blend membrane merged tightly with the surrounding tissues without gaps, and cells infiltrated and grew into the material. Moreover, the negative effects of superfine silk-fibroin powder/polyurethane blend membrane on cells were less than those of its polytetrafluoroethylene counterpart. Our findings indicated that the superfine silk-fibroin powder/polyurethane blend membrane has better histocompatibility than polytetrafluoroethylene membrane. It is concluded that the superfine silk-fibroin powder/polyurethane blend membrane is a promising biomaterial for small-diameter prosthesis.
Ouyang
Chenxi
C
Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ouyangchx@yahoo.com
Xu
Haiye
H
Wang
Weici
W
Yang
Hongjun
H
Xu
Weilin
W
D000818:Animals; D001672:Biocompatible Materials; D005349:Fibroins / Q000737:chemistry*; D020007:Implants, Experimental; D008567:Membranes, Artificial; D051379:Mice; D018482:Muscle, Skeletal / Q000601:surgery; D011140:Polyurethanes / Q000737:chemistry*; D011208:Powders; D011897:Random Allocation; D058017:Vascular Grafting
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011140:Polyurethanes; D011208:Powders; D005349:Fibroins