title: Unique alternative translation from two open reading frames on Acpin1 mRNA yields an acrosomal protein and a salivary-gland-specific protein.
abstract: OBJECTIVE
  To examine the expression profiles of the proteins translated from Acpin1 mRNA in germ cells.
  
  
  METHODS
  Northern and western blotting of various tissues and immunohistochemical analysis of germ cells were carried out in a mouse model.
  
  
  RESULTS
  ACPIN1 protein was transcribed from the longer, 3' open reading frame (ORF) of Acpin1. An alternative-splicing variant, Acpin1vs, contained only the smaller, 5' ORF of the full-length Acpin1 gene. Its gene product, SAGSIN1, was expressed specifically in salivary glands. Retrotransposed regions of Acpin1 homology were also detected in various chromosomes, and intronless paralogous genes on the X chromosome were expressed in the testis and other tissues. The genomic structure of Acpin1 is highly conserved in mammals.
  
  
  CONCLUSIONS
  The two ORFs on the Acpin1 mRNA are independently translated in differentiated cells. Analysis of gene Acpin1 might clarify the molecular mechanism of spermatogenesis.
authors: Tanaka Project, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka, Japan.
authors: Tomohiro
authors: T
authors: Ueda
authors: Hiroyuki
authors: H
authors: Manabe
authors: Keizo
authors: K
authors: Tokuhiro
authors: Mika
authors: M
authors: Hirose
authors: Yasuhiro
authors: Y
authors: Matsuoka
authors: Yasushi
authors: Y
authors: Miyagawa
authors: Akira
authors: A
authors: Tsujimura
authors: Kyoko
authors: K
authors: Fujita
authors: Morimasa
authors: M
authors: Wada
authors: Akihiko
authors: A
authors: Okuyama
authors: Yoshitake
authors: Y
authors: Nishimune
authors: Hiromitsu
authors: H
authors: Tanaka
mesh_terms: D000177:Acrosome / Q000502:physiology*; D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D018076:DNA, Complementary / Q000235:genetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016366:Open Reading Frames / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012469:Salivary Glands / Q000502:physiology*; D013091:Spermatogenesis / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; C545285:Fam220a protein, mouse; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger