Bacillus galliciensis sp. nov., isolated from faeces of wild seahorses (Hippocampus guttulatus).
A Gram-positive-staining, motile, rod-shaped, endospore-forming bacterium (BFLP-1( T)) was isolated from faeces of wild long-snouted seahorses ( Hippocampus guttulatus) captured in north-west Spain (Toralla, Galicia). Strain BFLP-1(T) grew at 10-30 degrees C and pH 5.5-9 (optimally at 20 degrees C and pH 7.2) and with 0-7 % (w/v) NaCl (optimally with 2 % NaCl). The G+C content of the DNA was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-1(T) was a member of the genus Bacillus and was most closely related to Bacillus herbersteinensis D-1,5a(T) (96.6 %), B. shackletonii LMG 18435(T) (96.0 %) and B. isabeliae CVS-8(T) (95.9 %). Chemotaxonomic data (peptidoglycan type, meso-diaminopimelic acid; major menaquinone, MK-7; predominant fatty acids, anteiso-C(15 : 0 ), anteiso-C(17 : 0) and C(16 : 1 )omega11c; major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminoglycophospholipid) supported the affiliation of strain BFLP-1(T) to the genus Bacillus . Comparative analysis of 16S rRNA gene sequences and chemotaxonomic and phenotypic features indicated that strain BFLP-1(T) represents a novel species within the genus Bacillus, for which the name Bacillus galliciensis sp. nov. is proposed. The type strain is BFLP-1( T) (=DSM 21539(T) =LMG 24668(T)).
Balcázar
José Luis
JL
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), c/. Eduardo Cabello 6, 36208 Vigo, Spain.
Pintado
José
J
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), c/. Eduardo Cabello 6, 36208 Vigo, Spain.
Planas
Miquel
M
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), c/. Eduardo Cabello 6, 36208 Vigo, Spain.
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D023701:Smegmamorpha / Q000382:microbiology*; D013030:Spain; D013045:Species Specificity
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S