Deep tissue multiphoton microscopy using longer wavelength excitation.
We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain. We achieved high contrast imaging of blood vessels at approximately twice the depth with 1280-nm excitation as with 775-nm excitation. An imaging depth of 1 mm can be achieved in in vivo imaging of adult mouse brains at 1280 nm with approximately 1-nJ pulse energy at the sample surface. Blood flow speed measurements at a depth of 900 mum are performed.
Kobat
Demirhan
D
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA. dk287@cornell.edu
Durst
Michael E
ME
Nishimura
Nozomi
N
Wong
Angela W
AW
Schaffer
Chris B
CB
Xu
Chris
C
D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008027:Light; D008029:Lighting / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D008962:Models, Theoretical; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity