Involvement of Pta1, Pcf11 and a KlCYC1 AU-rich element in alternative RNA 3'-end processing selection in yeast.
This work reports the involvement of yeast RNA processing factors Pta1 and Pcf11 in alternative 3'-end RNA processing. The pta1-1 and pcf11-2 mutations changed the predominance of KlCYC1 1.14 and 1.5 kb transcript isoforms. Mutation of the KlCYC1 3'-UTR AU-rich sequence at positions 679-690 (mutant M1) altered transcript predominance. Moreover, expression of M1 in the yeast mutants partially suppressed their effects in the predominance pattern. The combination of the M1 and M2 (694-698 deletion) mutations abolished the alternative processing. Pta1 involvement in this selection was confirmed using the Pta1-td degron strain.
Seoane
Silvia
S
Universidade da Coruña, Facultad de Ciencias, Campus da Zapateira S/N, 15071 A Coruña, Spain.
Lamas-Maceiras
Mónica
M
Rodríguez-Torres
Ana M
AM
Freire-Picos
María A
MA
D020413:3' Untranslated Regions; D017398:Alternative Splicing; D001483:Base Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D026723:Polyadenylation; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; C512637:PCF11 protein, S cerevisiae; C076229:PTA1 protein, S cerevisiae; D012322:RNA Precursors; D029701:Saccharomyces cerevisiae Proteins; D039221:mRNA Cleavage and Polyadenylation Factors