title: Comparison of photodynamic efficacy of tetraarylporphyrin pegylated or encapsulated in liposomes: in vitro studies.
abstract: Two photosensitizing systems: (1) tetrakis(4-hydroxyphenyl)porphyrin (p-THPP) encapsulated in sterically stabilized liposomes (SSL) and (2) p-THPP functionalized by covalent attachment of poly(ethylene glycol) (p-THPP-PEG(2000)) were studied in vitro. The dark and photo cytotoxicity of these systems were evaluated on two cell lines: HCT 116, a human colorectal carcinoma cell line, and DU 145, a prostate cancer cell line and compared with these determined for free p-THPP. It was demonstrated that both encapsulation in liposomes as well as attachment of PEG chain result in pronounced reduction of the dark cytotoxicity of the parent porphyrin. The liposomal formulation showed higher than p-THPP-PEG(2000) photocytotoxicity towards both cell lines used in the studies.
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland.
authors: Kinga
authors: K
authors: Nawalany
authors: Aleksandra
authors: A
authors: Rusin
authors: Mariusz
authors: M
authors: Kepczyński
authors: Alexei
authors: A
authors: Mikhailov
authors: Gabriela
authors: G
authors: Kramer-Marek
authors: Mirosław
authors: M
authors: Snietura
authors: Jan
authors: J
authors: Połtowicz
authors: Zdzisław
authors: Z
authors: Krawczyk
authors: Maria
authors: M
authors: Nowakowska
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008027:Light; D008081:Liposomes; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011166:Porphyrins / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*
chemical_list: D008081:Liposomes; D017319:Photosensitizing Agents; D011166:Porphyrins; C481734:meso-tetra-(4-hydroxyphenyl)porphyrin; D011092:Polyethylene Glycols