[Expression of iPLA2 in human pancreatic islets and its important role in glucose-stimulated insulin secretion].
OBJECTIVE
To assess the role of calcium-independent phospholipase A2 (iPLA2) in human pancreatic islets.


METHODS
The immunohistochemical analysis and Western blot were employed to examine iPLA2 expression in human pancreatic islets. Bromoenol lactone (BEL), a selective inhibitor of iPLA2, was used in a randomized controlled trial to compare its influence to glucose-stimulated insulin secretion.


RESULTS
iPLA2 was expressed predominantly in islet cells co-stained by insulin but was barely detected in the exocrine acinar cells. Western blot results indicated that islet cells expressed an iPLA2-immunoreactive band at the 80 kDa region. Glucose-stimulated insulin secretory response was dramatically reduced in islets pretreated with BEL (0.8285 +/- 0.0803 ng x islet(-1) x h(-1)) as compared with the control (1.2264 +/- 0.0568 ng x islet(-1) x h(-1)) (P < 0.01). BEL inhibited glucose stimulated insulin secretion from isolated human islets.


CONCLUSIONS
iPLA2 signaling plays an important role in glucose-stimulated insulin secretion under the physiological conditions.
Wang
Rui-tao
RT
Department of General Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China.
Song
Ke-ying
KY
Shi
Jie-yi
JY
Lu
Lie-sheng
LS
Yang
Jing
J
Wang
Qing-ping
QP
Li
Ke
K
D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000378:metabolism*; D009281:Naphthalenes / Q000494:pharmacology; D054512:Phospholipases A2, Calcium-Independent / Q000378:metabolism*; D011753:Pyrones / Q000494:pharmacology; D015398:Signal Transduction
D007328:Insulin; D009281:Naphthalenes; D011753:Pyrones; C068314:6-(bromomethylene)tetrahydro-3-(1-naphthaleneyl)-2H-pyran-2-one; D054512:Phospholipases A2, Calcium-Independent; D005947:Glucose