[Quantification of functional bacteria in one-step completely autotrophic nitrogen removal process influenced by dissolved oxygen].
OBJECTIVE
To study the influence of dissolved oxygen (DO) on bacterial population in a one-step autotrophic nitrogen removal process and to improve the system operation.


METHODS
Ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and anaerobic ammonium oxidizing bacteria (ANAMMOX) were amplified directly from the one-step completely autotrophic nitrogen removal reactor by using the specific PCR primers. The purified PCR products were cloned into T-vector and identified as the target fragments of AOB, NOB and ANAMMOX by sequencing. Recombined plasmid was used as standard molecule sample in Real-time PCR for quantification.


RESULTS
High DO was beneficial to AOB and NOB. The population of both AOB and NOB were higher in activated sludge than biofilm samples. High DO had the directly influence on ANAMMOX population whereas low DO had the inhibitory effect of ANAMMOX activity if the system was lack of NO3- and NO2-.


CONCLUSIONS
DO of 2.0 (aeration)/0.4(non-aeration) mg/L was the best operation concentration. Meanwhile, ANAMMOX population reached the peak in this condition and AOB, NOB as well as ANAMMOX composes of a steady co-metabolisms system.
Qin
Yu
Y
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China. qinyu54001@163.com
Fang
Fang
F
Guo
Jinsong
J
Yang
Guohong
G
Dong
Li
L
D052818:Autotrophic Processes; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012995:Solubility
D012336:RNA, Ribosomal, 16S; D009584:Nitrogen; D010100:Oxygen