title: Survival of avian H5N1 influenza A viruses in Calliphora nigribarbis (Diptera: Calliphoridae).
abstract: In a previous study, the highly pathogenic avian influenza (HPAI) H5N1 viruses were isolated from blow flies collected at the Tamba Town of Kyoto prefecture during the outbreak period in March 2004. In this study, we carried out virus exposure experiments to investigate whether the H5N1 virus would survive in a blow fly, Calliphora nigribarbis. The virus exposure experiments showed that the H5N1 influenza virus was isolated from the crop and intestine of C. nigribarbis for at least 24 h, and the viruses remained viable with titers ranging from 0.5 to 4.63 TCID50. This result suggests that C. nigribarbis could possibly transport the H5N1 virus over a distance of 2 km, which is the distance they can migrate within 24 h.
authors: Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. sawabe@nih.go.jp
authors: Kyoko
authors: K
authors: Sawabe
authors: Kiyoshi
authors: K
authors: Tanabayashi
authors: Akitoyo
authors: A
authors: Hotta
authors: Keita
authors: K
authors: Hoshino
authors: Haruhiko
authors: H
authors: Isawa
authors: Toshinori
authors: T
authors: Sasaki
authors: Akio
authors: A
authors: Yamada
authors: Hiromu
authors: H
authors: Kurahashi
authors: Chieko
authors: C
authors: Shudo
authors: Mutsuo
authors: M
authors: Kobayashi
mesh_terms: D000818:Animals; D004175:Diptera / Q000821:virology*; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification / Q000502:physiology*; D007303:Insect Vectors / Q000821:virology*; D007422:Intestines / Q000821:virology; D013997:Time Factors; D014779:Virus Replication