title: Composition of the fresh leaves and stems of Melissa officinalis and evaluation of skin irritation in a reconstituted human epidermis model.
abstract: The composition of a centrifuged product obtained from the fresh leaves and stems of Melissa officinalis and skin irritation in the reconstituted human epidermis (Episkin model) have been investigated in comparison to the EtOH-H(2)O (1:1) extract obtained by Soxhlet from the dried plant. Two new sulfated triterpenes (1 and 2) and two ionol derivatives have been isolated for the first time from Melissa officinalis together with caffeic and rosmarinic acids. The structures of compounds 1 and 2 were established by analysis of their spectroscopic data. Both the centrifuged material and its major constituents neither affected cell viability nor caused the release of pro-inflammatory mediators or the decrease of trans-epithelial electrical resistance (TEER) in the reconstituted human epidermis.
authors: Dipartimento di Scienze Farmaceutiche, University of Salerno, Via Ponte Don Melillo, 84084, Fisciano, Salerno, Italy.
authors: Teresa
authors: T
authors: Mencherini
authors: Patrizia
authors: P
authors: Picerno
authors: Paola
authors: P
authors: Russo
authors: Marisa
authors: M
authors: Meloni
authors: Rita
authors: R
authors: Aquino
mesh_terms: D004817:Epidermis / Q000187:drug effects; D006801:Humans; D007509:Irritants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031334:Melissa / Q000737:chemistry*; D008954:Models, Biological; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins; D012871:Skin Diseases / Q000209:etiology; D013463:Sulfuric Acid Esters / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: C543804:23-hydroxytormentic acid, 23 sulfate ester; D007509:Irritants; D012503:Saponins; D013463:Sulfuric Acid Esters; D014315:Triterpenes; C543803:niga-ichigoside F1, 23 sulfate ester