Effects of extracts from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on MSU crystal-induced rats gouty arthritis.
The effects of extract of Paederia scandens (LOUR.) MERRILL (Rubiaceae) (EPS), a Chinese traditional herbal medicine, on inflammatory and immune responses and their mechanisms in MSU crystals-induced (GA) rats were studied. GA rats were established. Ankle joint volume of rats was measured by volume meter; the level of TNF-alpha and IL-1beta was determined by radioimmunoassay. mRNA expressions of TNF-alpha and IL-1beta in synovial tissue of GA rats were analyzed by RT-PCR, and the expression of NF-kappaB was detected by immunohistochemistry. The administration of EPS (2.25, 4.5 g/kg, ig 9 days) inhibited the inflammatory response in GA rats. The mRNA expressions of TNF-alpha and IL-1beta were also significantly suppressed in synovial tissue. In addition, EPS (2.25, 4.5 g/kg, ig 9 days) inhibited the expression of TNF-alpha and IL-1beta and the biological activity of NF-kappaB. These results suggested that EPS possesses antiinflammatory effects by modulating pro-inflammatory mediators' production in synovial tissue and inactivating NF-kappaB pathway transmembrane signal transduction which plays a crucial role in the pathogenesis of this disease.
Ma
Ying
Y
Department of Pharmacology, Anhui Medical University, Hefei 230032, China.
Zhou
Lan-Lan
LL
Yan
Hai-Yan
HY
Liu
Mei
M
D000818:Animals; D015210:Arthritis, Gouty / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003460:Crystallization; D000081004:Cyclopentane Monoterpenes; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011714:Pyrans / Q000737:chemistry / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019666:Rubiaceae / Q000737:chemistry*; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014527:Uric Acid / Q000737:chemistry / Q000633:toxicity
D000081004:Cyclopentane Monoterpenes; D005960:Glucosides; D053583:Interleukin-1beta; D016328:NF-kappa B; D010936:Plant Extracts; D011714:Pyrans; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C543145:paederosidic acid methyl ester; D014527:Uric Acid; C077956:asperuloside