Bioinformatic comparisons and tissue expression of the neuronal nitric oxide synthase (nNOS) gene from the red drum (Sciaenops ocellatus).
The full length cDNA sequence for neuronal nitric oxide synthase (nNOS) gene from red drum (Sciaenops ocellatus) has been cloned, subjected to bioinformatic analysis, and examined for expression in different tissues. Red drum nNOS showed high identity to nNOS of mammals and other fish species. Notably, a unique 7-aa insertion was found in the important catalytic sites of the NO synthase domain, possibly affecting the function of red drum nNOS. Furthermore, this nNOS was expressed not only in brain but also in most of the internal organs including liver, intestine, spleen, head kidney and thymus.
Zhou
Libin
L
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou 510631, China; Department of Life Science, Huizhou University, Huizhou 516007, China.
Bai
Ru
R
Tian
Jianxiao
J
Liu
Xiaochun
X
Lu
Danqi
D
Zhu
Pei
P
Liu
Yun
Y
Zeng
Lujiao
L
Luo
Wenna
W
Zhang
Yong
Y
Wang
Anli
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D008969:Molecular Sequence Data; D052248:Nitric Oxide Synthase Type I / Q000235:genetics / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D010802:Phylogeny; D014018:Tissue Distribution
D018076:DNA, Complementary; D052248:Nitric Oxide Synthase Type I