title: Global transcriptional response of Salmonella enterica serovar Typhi to anti-z66 antiserum.
abstract: Recent studies have shown that flagella may modulate physiological processes by sensing environmental changes in temperature and moisture. When the z66(+) strain of Salmonella enterica serovar Typhi (S. Typhi) was exposed to an antiserum against the z66 flagellar antigen, the fljBA operon was deleted from a linear plasmid, leading to the unidirectional flagellar phase variation from FljB to FliC. We hypothesized that flagella may serve as a sensor that responded to the antiserum by altering gene expression and triggering the unidirectional flagellar phase variation. To test this hypothesis, Salmonella genomic DNA microarrays were used to determine the gene expression profile of the z66(+) wild-type strain of S. Typhi treated with the anti-z66 antiserum for 30 min. The results showed that expression levels of 187 genes were altered by more than threefold compared with the same strain treated with control serum. The microarray expression patterns of representative genes were validated by reverse transcriptase-PCR. Importantly, no significant changes in gene expression were observed in the fljB:z66 deletion mutant that was similarly treated with the anti-z66 antiserum. To the best of our knowledge, this is the first study to show the global transcriptional response of Salmonella to antiflagellin antiserum.
authors: Department of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Jiangsu, China.
authors: Haifang
authors: H
authors: Zhang
authors: Xiumei
authors: X
authors: Sheng
authors: Shungao
authors: S
authors: Xu
authors: Yulin
authors: Y
authors: Gao
authors: Hong
authors: H
authors: Du
authors: Junrong
authors: J
authors: Li
authors: Huaxi
authors: H
authors: Xu
authors: Xinxiang
authors: X
authors: Huang
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D005408:Flagellin / Q000235:genetics / Q000276:immunology; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D012485:Salmonella typhi / Q000276:immunology* / Q000502:physiology*; D013312:Stress, Physiological
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; C094427:FljB protein, Salmonella; C487817:z66 antigen, Salmonella enterica; D005408:Flagellin