title: Development of jejunal graft damage during intestinal transplantation.
abstract: BACKGROUND
  Intestinal transplantation (ITx) represents difficult life-saving intervention reserved for patients with irreversible intestinal failure. A serious complication of ITx is jejunal graft (JG) damage. The aim of the study was to evaluate the development of JG damage during ITx and determine the share of pathological elements (mechanical manipulation, ischemia, reperfusion) in this damage.
  
  
  METHODS
  Male Wistar rats (n=60; 30 donors and 30 recipients) were used. The harvest of JG as well as heterotopic allotransplantation was performed using a technique adapted from Balaz et al. (2003). In all transplantations, three samples of JG were obtained: immediately after harvest (Sa1), after preservation (Sa2) and 60min after transplantation (Sa3). The samples were stained using the Hematoxylin&Eosin method and histopathological injury index (HII) was assessed using Park/Chiu classification. For detection and quantification of neuroendocrine cells (NECs) Singh's modification of the Masson-Hamperl argentaffin technique was used.
  
  
  RESULTS
  The lowest level of HII was detected in Sa1=0.25+/-0.18; higher after preservation Sa2=1.42+/-0.38 and the highest HII was observed after transplantation Sa3=3.08+/-0.38. The percentage share of mechanical manipulation with the graft in jejunal damage during ITx was 8.11% (Sa1), the share of the ischemic element represented 37.98% (Sa2) and reperfusion had 53.91% of the share in jejunal damage (Sa3). The activity of NECs had sinusoidal character (Sa1=0.5+/-0.1; Sa2=1.4+/-0.0; Sa3=0.35+/-0.05).
  
  
  CONCLUSIONS
  The development of JG damage during ITx had progressive character. Mechanical manipulation had minimal influence on jejunal damage. One third of damage was caused by the ischemic component and the largest impact on JG damage resulted from reperfusion.
authors: Department of Histology and Embryology, Faculty of Medicine, P. J. Safárik University, Kosice, Slovakia. janko.varga@gmail.com
authors: Ján
authors: J
authors: Varga
authors: Pavel
authors: P
authors: Stasko
authors: Stefan
authors: S
authors: Tóth
authors: Zuzana
authors: Z
authors: Pristásová
authors: Martin
authors: M
authors: Bujdos
authors: Mikulás
authors: M
authors: Pomfy
mesh_terms: D000818:Animals; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D007422:Intestines / Q000473:pathology / Q000637:transplantation*; D007511:Ischemia / Q000473:pathology; D007583:Jejunum / Q000098:blood supply / Q000293:injuries* / Q000473:pathology / Q000637:transplantation; D008297:Male; D055099:Neuroendocrine Cells / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology; D014184:Transplantation, Homologous