Genome wide expression analysis of the effect of Pinelliae Rhizoma extract on psychological stress.
Pinelliae Rhizoma has been used traditionally as an antidepressant in Oriental medicine. In this study, the effect of Pinelliae Rhizoma extract (PRe) on psychological stress was investigated in mice. The results of an elevated plus-maze experiment revealed that application of psychological stress to mice led to the development of an abnormal behavioral pattern. However, oral administration of PRe significantly reduced the abnormal behavior of mice with a recovery rate of 75.5%. To elucidate the molecular mechanism by PRe, a microarray analysis of the brains of mice was conducted. The results of this analysis revealed that 456 genes were up-regulated and 392 genes were down-regulated in response to psychological stress. The expression of most of the genes that were altered in response to psychological stress was restored to normal levels in PRe treated mice, with a recovery rate of 81.5% and 85.2% being observed for up- and down-regulated genes, respectively. Finally, when the interaction network information was analysed, the recovery rate of the core node genes (46 up- and 29 down-regulated genes) in PRe treated mice was found to be over 95%, which indicates that this final set of genes may be the effective target of PRe.
Kim
Bu-Yeo
BY
Department of Constitutional Medicine Research, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejon 305-701, Republic of Korea.
Cho
Su Jin
SJ
Kim
Hyung-Woo
HW
Kim
Su-Youn
SY
Lim
Se-Hyun
SH
Kim
Kyeong-Ok
KO
Choi
Chang-Won
CW
Lim
Chi-Yeon
CY
Byun
Boo-Hyeong
BH
Cho
Su-In
SI
Lee
Guem-San
GS
D000818:Animals; D001921:Brain / Q000378:metabolism; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D008517:Phytotherapy; D030021:Pinellia / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D013315:Stress, Psychological / Q000188:drug therapy*
D010936:Plant Extracts