title: Characterisation of normal and cancer stem cells: one experimental paradigm for two kinds of stem cells.
abstract: The characterisation of normal stem cells and cancer stem cells uses the same paradigm. These cells are isolated by a fluorescence-activated cell sorting step and their stemness is assayed following implantation into animals. However, differences exist between these two kinds of stem cells. Therefore, the translation of the experimental procedures used for normal stem cell isolation into the research field of cancer stem cells is a potential source of artefacts. In addition, normal stem cell therapy has the objective of regenerating a tissue, while cancer stem cell-centred therapy seeks the destruction of the cancer tissue. Taking these differences into account is critical for anticipating problems that might arise in cancer stem cell-centred therapy and for upgrading the cancer stem cell paradigm accordingly.
authors: Centre de Recherches du Service de Santé des Armées, P.O. Box 87, 38702 La Tronche, France.
authors: Jean-François
authors: JF
authors: Mayol
authors: Corinne
authors: C
authors: Loeuillet
authors: Francis
authors: F
authors: Hérodin
authors: Didier
authors: D
authors: Wion
mesh_terms: D000818:Animals; D015415:Biomarkers; D002469:Cell Separation; D006801:Humans; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000378:metabolism*; D010641:Phenotype; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism*
chemical_list: D015415:Biomarkers