A role for forkhead box A1 in acute lung injury.
Forkhead box protein A1 (FoxA1) is an evolutionarily conserved winged helix transcription factor with diverse regulatory functions. However, little is known about the role of FoxA1 in acute lung injury (ALI) and pulmonary cell injury. In this study, an in vivo model was employed whereby rats were administered an intravenous injection of oleic acid (OA, 0.1 ml/kg), and alveolar type II epithelial cells (AT-2 cells) injury was induced by hydrogen peroxide (H(2)O(2)) in vitro. OA injection resulted in lung injury and AT-2 cells apoptosis in vivo. OA injection and H(2)O(2) upregulated FoxA1 mRNA and protein in lung tissue of the in vivo ALI model and in H(2)O(2) challenged AT-2 cells. Overexpression of FoxA1 promoted apoptosis, whereas FoxA1 deficiency, induced by antisense oligonucleotides, decreased AT-2 cells apoptosis induced by H(2)O(2), as shown by flow cytometry. These results suggest that FoxA1 may play an important role in ALI by promoting apoptosis of pulmonary epithelial cells.
Song
Lan
L
Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Hunan, People's Republic of China.
Zhang
Bin
B
Feng
Yansheng
Y
Luo
Xinjing
X
Wei
Xing
X
Xiao
Xianzhong
X
D055371:Acute Lung Injury / Q000473:pathology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D051541:Hepatocyte Nuclear Factor 3-alpha / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D008297:Male; D016877:Oxidants / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D051541:Hepatocyte Nuclear Factor 3-alpha; D016877:Oxidants; D006861:Hydrogen Peroxide; D053148:Caspase 3