title: Measuring tendon properties in mdx mice: cell viability and viscoelastic characteristics.
abstract: Muscular dystrophy is a genetic disorder of skeletal muscle characterized by progressive muscle weakness. Here we assessed whether muscle wasting affects cell viability and mechanical properties of extensor digitorum longus (EDL) and of tibialis anterior (TA) tendons from mdx dystrophic mice compared to wild type (WT) mice. mdx mice represent the classical animal model for human Duchenne muscular dystrophy, and show several signs of the pathology, including a decrease in specific force and an increase of fibrotic index. Cell viability of tendons was evaluated by histological analysis, and viscoelastic properties have been assessed by a rapid measurement protocol that allowed us to compute, at the same time, tissue complex compliance for all the frequencies of interest. Confocal microscopy and mechanical properties measurements revealed that mdx tendons, compared to WT ones, have an increase in the number of dead cells and a significant reduction in tissue elasticity for all the frequencies that were tested. These findings indicate a reduced quality of the tissue. Moreover, mdx tendons have an increase in the viscous response, indicating that during dynamic loading, they dissipate more energy compared to WT. Our results demonstrate that muscular dystrophy involves not only muscle wasting, but also alteration in the viscoelastic properties of tendons, suggesting a paracrine effect of altered skeletal muscle on tendinous tissue.
authors: Institute Pasteur Cenci-Bolognetti, Department of Histology and Medical Embryology, CE-BEMM, Sapienza University of Rome, 00161 Rome, Italy.
authors: E
authors: E
authors: Rizzuto
authors: A
authors: A
authors: Musarò
authors: A
authors: A
authors: Catizone
authors: Z
authors: Z
authors: Del Prete
mesh_terms: D000818:Animals; D002470:Cell Survival; D003198:Computer Simulation; D004195:Disease Models, Animal; D055119:Elastic Modulus; D006801:Humans; D051379:Mice; D018101:Mice, Inbred mdx; D008954:Models, Biological; D009136:Muscular Dystrophies / Q000473:pathology* / Q000503:physiopathology*; D013314:Stress, Mechanical; D013710:Tendons / Q000473:pathology* / Q000503:physiopathology*; D013718:Tensile Strength; D014783:Viscosity