Polysaccharide/polyaminoacid composite scaffolds for modified DNA release.
In this work composite polymeric films or sponges, based on hyaluronic acid (HA) covalently crosslinked with alpha,beta-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)-D,L-aspartamide (PE), have been prepared and characterized as local gene delivery systems. In particular, HA/PE scaffolds have been loaded with PE/DNA interpolyelectrolyte complexes, employing PE as a macromolecular crosslinker for HA and as a non-viral vector for DNA. In vitro studies showed that HA/PE films and sponges have high compatibility with human dermal fibroblasts and they give a sustained DNA release, whose trend can be easily tailored by varying the crosslinking ratio between HA and PE. Electrophoresis analysis and transfection studies on B16-F10 cells revealed that DNA is released as a complex with PE and it retains its bioactivity.
Pitarresi
G
G
Dipartimento di Chimica e Tecnologie Farmaceutiche, Università di Palermo, via Archirafi 32, 90123, Palermo, Italy. giopitar@unipa.it
Calabrese
R
R
Palumbo
F S
FS
Licciardi
M
M
Giammona
G
G
D000818:Animals; D001218:Aspartame / Q000031:analogs & derivatives* / Q000737:chemistry; D001692:Biological Transport; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D007700:Kinetics; D008546:Melanoma, Experimental / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011073:Polyamines / Q000737:chemistry*; D012995:Solubility; D014162:Transfection / Q000379:methods*
D003432:Cross-Linking Reagents; D011073:Polyamines; C509366:alpha,beta-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)-d,l-aspartamide; D006820:Hyaluronic Acid; D004247:DNA; D006821:Hyaluronoglucosaminidase; D001218:Aspartame