[Effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro].
OBJECTIVE
To investigate the effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro.


METHODS
Human epidermal squamous cell carcinoma cell line A431 was cultured. After the treatment with triptolide, the inhibition of cellular growth was determined by measuring MTT dye absorption of the living cells. Light microscope showed morphological changes. The cell cycle and apoptosis rate were assessed by flow cytometry.


RESULTS
Triptolide could significantly inhibit the proliferation of A431 cells in a dose- and time-dependent manner. Triptolide could also cause cell morphological changes (the number of floating cells and nuclear pyknosis increase), induce cell apoptosis, and change the distribution of cell cycle phase in A431 cells. Compared with the control group, the G( 0) /G( 1) phase A431 cell rate increased and the rate of S phase cell decreased in TP-treated group. Cell cycles were obviously inhibited by triptolide in G( 0) /G( 1) phase ( both P<0.05).


CONCLUSIONS
TP could play an anti-tumor role by effectively inducing cell apoptosis and inhibiting the proliferation of A431 cells.
Chen
Mingliang
M
Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Tan
Shuai
S
Zhang
Guiying
G
Yi
Mei
M
Jian
Dan
D
Xie
Hongfu
H
Chen
Xiang
X
D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D010616:Phenanthrenes / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000473:pathology*
D018906:Antineoplastic Agents, Alkylating; D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide