Expression pattern of mda-7/IL-24 receptors in liver cancer cell lines.
BACKGROUND
The mda-7/IL-24 receptor belongs to the type II cytokine receptor family, and its two heterodimeric receptors are IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Mda-7/IL-24 receptor expression in liver cancer cell lines has not yet been described. This information may be helpful for further clinical gene therapy.


METHODS
With normal skin total RNA as template, the cDNA sequences of IL-20R1, IL-20R2 and IL-22R were amplified by RT-PCR. Total RNA was extracted from cultured liver cancer cell lines and a normal liver cell line, then detected by northern blotting, and the expression of mda-7/IL-24 receptors was analyzed.


RESULTS
PLC/PRF/5 and SMMC-7721 expressed IL-20R1; BEL-7402, Hep3B, HepG2, and PLC/PRF/5 expressed IL-20R2; and HepG2 and PLC/PRF/5 expressed IL-22R. Only HepG2 expressed the IL-22R/IL-20R2 receptor complex. PLC/PRF/5 completely expressed both heterodimeric receptors. Huh-7, QGY-7701 and WRL-68 did not express the IL-24 receptor.


CONCLUSIONS
Complete mda-7/IL-24 receptors are seldom expressed in liver cancer cell lines.
Zhu
Hong
H
Abdominal Cancer Centre, Third Affiliated Hospital, Kunming Medical College, Kunming 650102, China.
Yang
Zhi-Bin
ZB
D015152:Blotting, Northern; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D055503:Protein Multimerization; D018123:Receptors, Interleukin / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099180:Interleukin-24
D007378:Interleukins; D018123:Receptors, Interleukin; D000099180:Interleukin-24; C492510:interleukin-20 receptor; C415369:interleukin-22 receptor