title: S100A4 mRNA is a diagnostic and prognostic marker in pancreatic carcinoma.
abstract: OBJECTIVE
  The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer.
  
  
  METHODS
  We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028).
  
  
  CONCLUSIONS
  S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.
authors: Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Fukuoka, 812-8582, Japan.
authors: Naoki
authors: N
authors: Ikenaga
authors: Kenoki
authors: K
authors: Ohuchida
authors: Kazuhiro
authors: K
authors: Mizumoto
authors: Jun
authors: J
authors: Yu
authors: Hayato
authors: H
authors: Fujita
authors: Kohei
authors: K
authors: Nakata
authors: Junji
authors: J
authors: Ueda
authors: Norihiro
authors: N
authors: Sato
authors: Eishi
authors: E
authors: Nagai
authors: Masao
authors: M
authors: Tanaka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D021441:Carcinoma, Pancreatic Ductal / Q000175:diagnosis* / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000235:genetics*; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C077591:S100A4 protein, human