Shortening-induced force depression is primarily caused by cross-bridges in strongly bound states.
The steady-state isometric force following active muscle shortening is smaller than the corresponding force obtained for purely isometric contractions. This so-called residual force depression has been observed consistently for more than half a century, however its mechanism remains a matter of scientific debate. [Maréchal, G., Plaghki, L., 1979. The deficit of the isometric tetanic tension redeveloped after a release of frog muscle at a constant velocity. J. Gen. Physiol. 73, 453-467] suggested that force depression might be caused by alterations in the cross-bridge kinetics following muscle shortening, but there is no research studying force depression systematically for altered cross-bridge kinetic conditions. The purpose of this study was to investigate if force depression affects so-called weakly and strongly bound cross-bridges to the same degree. In order to achieve this aim, we modified the ratio of weakly to strongly bound cross-bridges with 2,3-butanedione monoxime (BDM) in single frog fibers. BDM inhibits the formation of strongly bound cross-bridges in a dose-dependent manner, thus the ratio of weakly to strongly bound cross-bridges could be altered in a systematic way. We found that the absolute amount of force depression was decreased by 50% while the relative amount was decreased by 12% in BDM exposed fibers compared to fibers in normal Ringer's solution. Furthermore, force depression was accompanied by a decrease in stiffness that was much greater in normal compared to BDM exposed fibers, leading to the conclusion that force depression was caused by an inhibition of cross-bridge attachment following fiber shortening and that this inhibition primarily affected cross-bridges in the strongly bound states.
Lee
Eun-Jeong
EJ
Faculty of Kinesiology, University of Calgary 2500 University Drive N.W., Calgary AB T2N 1N4, Canada.
Herzog
Walter
W
D000818:Animals; D002478:Cells, Cultured; D003198:Computer Simulation; D007537:Isometric Contraction / Q000502:physiology*; D008953:Models, Anatomic; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000502:physiology*; D011894:Rana pipiens; D012518:Sarcomeres / Q000502:physiology* / Q000648:ultrastructure*; D013314:Stress, Mechanical