Porticoccus litoralis gen. nov., sp. nov., a gammaproteobacterium isolated from the Yellow Sea.
A marine bacterium, designated IMCC2115(T), was isolated from coastal seawater (Yellow Sea, Korea) using a high throughput cultivation method based on dilution-to-extinction, and taxonomically investigated. Cells of the strain formed tiny, beige to off-white colonies and were Gram-stain-negative, obligately aerobic, chemoheterotrophic, non-motile cocci. Based on 16S rRNA gene sequence comparisons, the strain was most closely related to the genera Marinimicrobium (92.0-92.4 %) and Microbulbifer (91.6-92.8 %), but phylogenetic trees showed that the strain formed a distinct phyletic line in the class Gammaproteobacteria adjacent to the OM60 and SAR92 clades. The DNA G+C content of the strain was 47.8 mol% and the predominant cellular fatty acids were anteiso-C(15 : 0) (67.6 %), anteiso-C(17 : 0) (14.4 %) and C(16 : 0) (6.9 %). The 16S rRNA gene sequence analyses and phenotypic and chemotaxonomic tests allowed the differentiation of IMCC2115(T) from other related genera in the class Gammaproteobacteria. Therefore, strain IMCC2115(T) (=KCCM 42369(T) =NBRC 102686(T)) is proposed as the representative of a new genus and species, for which the name Porticoccus litoralis gen. nov., sp. nov. is proposed.
Oh
Hyun-Myung
HM
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Kim
Hana
H
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Kim
Kyung-Mi
KM
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Min
Gi-Sik
GS
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Cho
Jang-Cheon
JC
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S