title: Clioquinol and other hydroxyquinoline derivatives inhibit Abeta(1-42) oligomer assembly.
abstract: Soluble oligomeric amyloid-beta (Abeta) species are toxic to many cell types and are a putative etiological factor in Alzheimer's disease. The NINDS-Custom Collection of 1040 drugs and biologically active compounds was robotically screened for inhibitors of Abeta oligomer formation with a single-site biotinylated Abeta(1-42) oligomer assembly assay. Several quinoline-like compounds were identified with IC(50)'s <10 microM, including the antiprotozoal clioquinol that has been reported to have effects on metal ion metabolism. The 2-OH, 4-OH, and 6-OH quinolines do not block Abeta oligomer formation up to a concentration of 100 microM. Analogs of clioquinol have shown activity in reducing Abeta levels and improving behavioral deficits in mouse models of Abeta pathology. The inhibitory effects of clioquinol and other 8-OH quinoline derivatives on oligomer formation in vitro are unrelated to their chelating activity. Crosslinking studies suggest that clioquinol acts at the stage of trimer formation. These preliminary data may suggest that 8-OH quinolines have the potential for suppressing Abeta oligomer formation which should be considered when assessing the effects of these compounds in animal models and clinical trials.
authors: Department of Cellular and Molecular Biochemistry, Center on Aging, University of Kentucky, 800 S. Limestone Street, Lexington, KY 40536-0230, USA. hlevine@email.uky.edu
authors: Harry
authors: H
authors: LeVine
authors: Qunxing
authors: Q
authors: Ding
authors: John A
authors: JA
authors: Walker
authors: Randal S
authors: RS
authors: Voss
authors: Corinne E
authors: CE
authors: Augelli-Szafran
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism*; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000494:pharmacology; D001360:Avidin; D019921:Biotinylation; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D007464:Clioquinol / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004591:Electrophoresis, Polyacrylamide Gel; D006912:Hydroxyquinolines / Q000737:chemistry / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D055503:Protein Multimerization / Q000187:drug effects*; D054852:Small Molecule Libraries
chemical_list: D016229:Amyloid beta-Peptides; D000981:Antiprotozoal Agents; D002614:Chelating Agents; D006912:Hydroxyquinolines; D010446:Peptide Fragments; D054852:Small Molecule Libraries; C075222:amyloid beta-protein (1-42); D001360:Avidin; D007464:Clioquinol