title: Copper acquisition by the SenC protein regulates aerobic respiration in Pseudomonas aeruginosa PAO1.
abstract: Aerobic respiration of Pseudomonas aeruginosa involves four terminal oxidases belonging to the heme-copper family (that is, three cytochrome c oxidases and one quinol oxidase) plus one copper-independent, cyanide-insensitive quinol oxidase (CIO). The PA0114 gene encoding an SCO1/SenC-type protein, which is known to be important for copper delivery to cytochrome c in yeast, Rhodobacter spp. and Agrobacterium tumefaciens, was found to be important for copper acquisition and aerobic respiration in P. aeruginosa. A PA0114 (senC) mutant grew poorly in low-copper media and had low cytochrome cbb(3)-type oxidase activity, but expressed CIO at increased levels, by comparison with the wild-type PAO1. Addition of copper reversed these phenotypes, suggesting that periplasmic copper capture by the SenC protein helps P. aeruginosa to adapt to copper deprivation.
authors: Département de Microbiologie Fondamentale, Université de Lausanne, Lausanne, Switzerland.
authors: Emanuela
authors: E
authors: Frangipani
authors: Dieter
authors: D
authors: Haas
mesh_terms: D000332:Aerobiosis; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D003300:Copper; D010088:Oxidoreductases