title: Tumourigenic effect of Schistosoma haematobium total antigen in mammalian cells.
abstract: Schistosoma haematobium is endemic in several regions of Africa and has been shown to be associated with predominantly squamous cell bladder carcinoma. The mechanisms underlying the association between S. haematobium and bladder squamous cell carcinoma is largely unknown. All the reports so far, demonstrate exclusively an epidemiological evidence linking S. haematobium infection with squamous cell bladder carcinoma. We hypothesized that these parasite antigens might induce tumourigenesis. For this, we used normal mammalian cells of Chinese hamster ovary (CHO) and treated the cells in culture with S. haematobium total antigen (Sh). Our results showed increased proliferation in Sh-treated cells in comparison with the controls. The CHO cells exposed to Sh were inoculated subcutaneously into male nude mice and formed sarcomas (n = 5/5). The cells from the sarcomas expressed vimentin filaments and were negative to cytokeratin. Our results demonstrate for the first time that S. haematobium antigens induce tumour development in nude mice.
authors: CIMP - Centre for Parasite Immunology and Biology, National Institute of Health, 4000-055 Porto, Portugal. monicabotelho@hotmail.com
authors: Monica
authors: M
authors: Botelho
authors: Paula
authors: P
authors: Oliveira
authors: Joana
authors: J
authors: Gomes
authors: Fatima
authors: F
authors: Gartner
authors: Carlos
authors: C
authors: Lopes
authors: Jose Manuel Correia
authors: JM
authors: da Costa
authors: Jose Carlos
authors: JC
authors: Machado
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000494:pharmacology*; D034961:Antigens, Nuclear / Q000032:analysis; D016466:CHO Cells; D015197:Carcinogenicity Tests; D002294:Carcinoma, Squamous Cell / Q000382:microbiology* / Q000473:pathology; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D011897:Random Allocation; D012548:Schistosoma haematobium / Q000276:immunology / Q000472:pathogenicity*; D012553:Schistosomiasis haematobia / Q000150:complications* / Q000473:pathology; D014183:Transplantation, Heterologous; D001749:Urinary Bladder Neoplasms / Q000382:microbiology* / Q000473:pathology
chemical_list: D000947:Antigens, Helminth; D034961:Antigens, Nuclear; C051963:nucleolar organizer region associated proteins