Phage display selection, analysis, and prediction of B cell epitopes.
Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided.
Freund
Natalia Tarnovitski
NT
Tel Aviv University, Tel Aviv, Israel.
Enshell-Seijffers
David
D
Harvard Medical School, Charlestown, Massachusetts.
Gershoni
Jonathan M
JM
Tel Aviv University, Tel Aviv, Israel.
D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D001671:Biochemistry; D032701:Biomimetics; D019295:Computational Biology; D003198:Computer Simulation; D018604:Epitope Mapping / Q000379:methods*; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005818:Genetic Engineering; D006801:Humans; D017912:Inovirus; D018716:Molecular Mimicry; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D019151:Peptide Library
D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D010446:Peptide Fragments; D019151:Peptide Library