Strand invasion of extended, mixed-sequence B-DNA by gammaPNAs.
In this communication, we show that peptide nucleic acids (PNAs) with lengths of 15-20 nucleotides, when preorganized into a right-handed helix, can invade mixed-sequence double-helical B-form DNA (B-DNA). Strand invasion occurs in a highly sequence-specific manner through direct Watson-Crick base pairing. Unlike the previously developed double-duplex invasion strategy, which requires simultaneous binding of two strands of pseudocomplementary PNAs to DNA, only a single strand of gammaPNA is required for invasion in this case, and no nucleobase substitution is needed.
He
Gaofei
G
Department of Chemistry and Center for Nucleic Acids Science and Technology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA.
Rapireddy
Srinivas
S
Bahal
Raman
R
Sahu
Bichismita
B
Ly
Danith H
DH
D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D007700:Kinetics; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D020135:Peptide Nucleic Acids; D004247:DNA; D004262:DNA Restriction Enzymes