title: Characterization of sugars from model and enzyme-mediated pulp hydrolyzates using high-performance liquid chromatography coupled to evaporative light scattering detection.
abstract: High-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector was used to quantitatively determine glucose and cellobiose in hydrolyzates from the production of cellulose nanofillers from modified lignocellulosic materials. Prevail Carbohydrate ES 5 micron column proved more suitable for achieving the chromatographic separation of the model pulp hydrolyzate into its constituent sugars than the YMC-Pack Polyamine column. Linear calibration curves for the various sugars in the mixtures were developed. Glucose and cellobiose were clearly detectable in pulp hydrolyzates obtained from enzyme-mediated hydrolysis of recycled pulp, pine and hardwood dissolving pulps. Finally, the amount of glucose in the pulp hydrolyzates was generally higher than cellobiose.
authors: West Virginia University, Davis College of Agriculture, Forestry and Consumer Sciences, Division of Forestry and Natural Resources, 323 Percival Hall, Morgantown, WV 26506, United States. pfilson@mix.wvu.edu
authors: Paul B
authors: PB
authors: Filson
authors: Benjamin E
authors: BE
authors: Dawson-Andoh
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002480:Cellulase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006868:Hydrolysis; D008027:Light; D012542:Scattering, Radiation; D013997:Time Factors; D014835:Volatilization
chemical_list: D002241:Carbohydrates; D002480:Cellulase