Differentiation of Staphylococcus aureus strains by CE, zeta potential and coagulase gene polymorphism.
Staphylococcus aureus is a common cause of infection in both hospitals and the community, and it is becoming increasingly virulent and resistant to antibiotics. Possibilities of fast, sensitive and cheap determination of these pathogenic bacteria are extremely important in antimicrobial therapy. In the present study, CE with chemically modified capillary and zeta potential measurements were used for differentiation of three different clinical strains of S. aureus. The data presented in this contribution suggested that electrophoretic behavior and the values of zeta potential should be very useful in distinguishing between closely related strains, which exhibited coagulase gene/protein polymorphism. Understanding the differences between S. aureus strains could help to improve our knowledge about S. aureus pathogenecity and to monitor for and respond to emergence of more virulent strains.
Kłodzińska
Ewa
E
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
Szumski
Michał
M
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
Hrynkiewicz
Katarzyna
K
Department of Microbiology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń, Poland.
Dziubakiewicz
Ewelina
E
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
Jackowski
Marek
M
Department of Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Toruń, Poland.
Buszewski
Bogusław
B
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
D000704:Analysis of Variance; D003030:Coagulase / Q000235:genetics*; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D011110:Polymorphism, Genetic; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000737:chemistry / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D003030:Coagulase
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