Activity-linked labeling of enzymes by self-immolative polymers.
The development and application of tools that allow labeling of proteins in vitro and in vivo is a highly active and interdisciplinary area of research. Self-immolative polymers (SIPs) are a unique type of molecules that respond to external stimuli by undergoing head-to-tail disassembly through a self-immolative fragmentation. We have demonstrated how these polymers can be applied as activity-linked labeling probes for proteins with catalytic activity. A real-time detection of the protein labeling can be monitored due to a change in the fluorescence emission wavelength of the probe during the polymer disassembly process. The SIP-based labeling approach showed considerable conservation of catalytic activity of the labeled protein and significant preference toward labeling of the activating protein.
Weinstain
Roy
R
School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
Baran
Phil S
PS
Shabat
Doron
D
D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism
D004798:Enzymes; D005456:Fluorescent Dyes; D015335:Molecular Probes; D011108:Polymers