title: Cytological analysis of meiosis in fixed Drosophila ovaries.
abstract: Methods are described to analyze two different parts of the Drosophila ovary, which correspond to early stages (pachytene) and late stages (metaphase I and beyond) of meiosis. In addition to taking into account morphology, the techniques differ by fixation conditions and the method to isolate the tissue. Most of these methods are whole mounts, which preserve the three-dimensional structure.
authors.affiliation: Waksman Institute and Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.
authors.forename: Kim S
authors.initials: KS
authors.lastname: McKim
authors.forename: Eric F
authors.initials: EF
authors.lastname: Joyce
authors.forename: Janet K
authors.initials: JK
authors.lastname: Jang
mesh_terms: D000818:Animals; D020732:Cytogenetic Analysis / Q000379:methods; D003584:Cytological Techniques / Q000379:methods*; D004330:Drosophila / Q000166:cytology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology; D005260:Female; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D010053:Ovary / Q000166:cytology* / Q000378:metabolism; D016707:Tissue Fixation / Q000379:methods