Ret-dependent cell rearrangements in the Wolffian duct epithelium initiate ureteric bud morphogenesis.
While the genetic control of renal branching morphogenesis has been extensively described, the cellular basis of this process remains obscure. GDNF/RET signaling is required for ureter and kidney development, and cells lacking Ret are excluded from the tips of the branching ureteric bud in chimeric kidneys. Here, we find that this exclusion results from earlier Ret-dependent cell rearrangements in the caudal Wolffian duct, which generate a specialized epithelial domain that later emerges as the tip of the primary ureteric bud. By juxtaposing cells with elevated or reduced RET activity, we find that Wolffian duct cells compete, based on RET signaling levels, to contribute to this domain. At the same time, the caudal Wolffian duct transiently converts from a simple to a pseudostratified epithelium, a process that does not require Ret. Thus, both Ret-dependent cell movements and Ret-independent changes in the Wolffian duct epithelium contribute to ureteric bud formation.
Chi
Xuan
X
Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA.
Michos
Odyssé
O
Shakya
Reena
R
Riccio
Paul
P
Enomoto
Hideki
H
Licht
Jonathan D
JD
Asai
Naoya
N
Takahashi
Masahide
M
Ohgami
Nobutaka
N
Kato
Masashi
M
Mendelsohn
Cathy
C
Costantini
Frank
F
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002678:Chimera / Q000033:anatomy & histology / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology; D004848:Epithelium / Q000033:anatomy & histology / Q000196:embryology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009024:Morphogenesis / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D019076:Transgenes; D014513:Ureter / Q000033:anatomy & histology / Q000196:embryology*; D014928:Wolffian Ducts / Q000033:anatomy & histology* / Q000196:embryology* / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D008164:Luminescent Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; C425468:Spry1 protein, mouse; D051096:Proto-Oncogene Proteins c-ret; C494999:Ret protein, mouse
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