[Effects of 8-Methoxypsoralen on intracellular [Ca(2+)]i and cytoskeleton actin organization in human melanocytes in vitro].
OBJECTIVE
To investigate the effects of 8-methoxypsoralen on human melanocytes [Ca(2+)]i and cytoskeleton actin organization in vitro.


METHODS
Human melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca(2+)]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin.


RESULTS
8-methoxypsoralen increased [Ca(2+)]i and induced organization of actin stress fiber cytoskeleton.


CONCLUSIONS
8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca(2+) concentration and cytoskeleton actin reorganization.
ZHANG
Xian-qi
XQ
Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
ZHENG
Min
M
MOU
Kuan-hou
KH
FENG
Jie
J
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008730:Methoxsalen / Q000494:pharmacology*; D012867:Skin / Q000166:cytology
D000199:Actins; D003598:Cytoskeletal Proteins; D002118:Calcium; D008730:Methoxsalen