HIV type 1 diversity from newly diagnosed patients in Santos metropolitan area/Brazil.
HIV-1 from infected subjects has been characterized in order to provide a more accurate view of the strains that are currently found in a given region. In this report, we focused on characterizing the pol gene diversity obtained from newly diagnosed patients in Santos metropolitan area, Brazil. This region is composed of nine cities and an international port. Analysis of the 33 samples revealed that 22 strains belonged to subtype B, 4 to subtype F, and 2 to subtype C; 5 strains were B/F recombinants. Our results demonstrated that 18.2% of samples were primary antiretroviral resistance genotypic mutations, with high-level resistance to reverse transcriptase inhibitors in both subtypes B and F and in recombinant forms B/F. Our data revealed that the primary antiretroviral resistance genotypic mutations should be carefully investigated in developing countries with widespread access to antiretrovirals, such as Brazil.
de Sa-Filho
Dercy José
DJ
Centro Universitário Lusíada (UNILUS) , Núcleo Acadêmico de Estudos e Pesquisas em Virologia/Laboratório de Biologia Molecular, Boqueirão, Santos SP, Brazil 11045-101. dercy.sa@unifesp.br
Ambar
Rafael Favero
RF
Duarte
Natalia Brenneken
NB
Matias
Rafael Bragança Rodrigues
RB
Candido
Valéria
V
Gagliani
Luiz Henrique
LH
Caseiro
Marcos Montani
MM
D000328:Adult; D019380:Anti-HIV Agents / Q000494:pharmacology; D001938:Brazil; D016000:Cluster Analysis; D024882:Drug Resistance, Viral; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014505:Urban Population; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D054302:pol Gene Products, Human Immunodeficiency Virus