title: Metabolic changes of salicylic acid-elicited Catharanthus roseus cell suspension cultures monitored by NMR-based metabolomics.
abstract: The effect of salicylic acid (SA) on the metabolic profile of Catharanthus roseus suspension cells throughout a time course (0, 6, 12, 24, 48 and 72 h after treatment) was investigated using NMR spectroscopy and multivariate data analysis. When compared to control cell lines, SA-treated cells showed a high level of sugars (glucose and sucrose) up to 48 h after treatment, followed by a dynamic change in amino acids, phenylpropanoids, and tryptamine. Additionally, one compound--2,5-dihydroxybenzoic-5-O-glucoside--was detected solely in SA-treated cells.
authors.affiliation: Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.
authors.forename: Natali Rianika
authors.initials: NR
authors.lastname: Mustafa
authors.forename: Hye Kyong
authors.initials: HK
authors.lastname: Kim
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Choi
authors.forename: Robert
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D030008:Catharanthus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D005841:Gentisates / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055432:Metabolomics; D020005:Propanols / Q000032:analysis; D020156:Salicylic Acid / Q000378:metabolism*; D013997:Time Factors; D014363:Tryptamines / Q000032:analysis
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D005841:Gentisates; D020005:Propanols; D014363:Tryptamines; C439395:1-phenylpropanol; C030820:tryptamine; D020156:Salicylic Acid
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