[Changes of DDAs content affected by different processing time and its relationship with safety of processed Fuzi].
OBJECTIVE
To study the correlation between content changes of Diester diterpenoid alkaloids (DDAs) content and safety of the processed Fuzi.


METHODS
Sequential and Bliss methods were used to evaluate the safety of 7 kinds of Fuzi processed with different processing time. The relationship between ED50, TD50, TI and content changes of DDAs of those processed Fuzi was studied, the correlation between the content changes and effect of different processed Fuzi was analyzed, and the toxicity of those processed Fuzi with multiple linear regression was tested.


RESULTS
Fuzi with good efficiency and safety contains proper hypaconitine (HA) and mesaconitine (MA). Aconitine (AC) interfered efficacy of Fuzi (negative correlation), HA showed positive correlation with toxicity and efficacy of Fuzi.


CONCLUSIONS
HA and MA kept in determinate proportion are very important for the safety and effectivity of processed Fuzi.
Li
Zhiyong
Z
School of Chinese Pharmacy of Beijing University of Chinese Medicine, Beijing 100102, China.
Zhang
Shuofeng
S
Chang
Hongsheng
H
Sun
Jianning
J
Li
Fei
F
D000157:Aconitine / Q000031:analogs & derivatives / Q000378:metabolism; D000470:Alkaloids / Q000737:chemistry*; D000818:Animals; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D064420:Drug-Related Side Effects and Adverse Reactions; D004365:Drugs, Chinese Herbal / Q000009:adverse effects* / Q000378:metabolism* / Q000494:pharmacology; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D051381:Rats; D013997:Time Factors
D000470:Alkaloids; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine