[Effect of gushen pelyuan recipe on Bcl-2 and Bax mRNA expressions in cochlea tissue of rats with Shen-yang deficiency syndrome].
OBJECTIVE
To study the effect of Gushen Peiyuan Recipe (GPR) on the expression of Bcl-2 and Bax mRNA in Shen-yang-deficiency (SYD) rats suffering from cochlea apoptosis, thus providing a theoretical basis to the treatment and prevention of sensorineural hearing loss and fill Chinese medine's theory of kidney-ear-correlation with new substance.


METHODS
Rats were induced into experimental SYD animal models by injecting cetacort into their buttocks. Rats in the blank and model groups were given 10 mL/kg normal saline by gastrogavage, and 31 g/kg, 15.5 g/kg and 7.5 g/kg GPR were given to the rats in the high, medium and low dose groups by gastrogavage respectively. RT-PCR was adopted to detect the mRNA expressions of Bcl-2 and Bax.


RESULTS
Levels of Bcl-2 mRNA expression and Bcl-2/Bax enhanced, and mRNA expression of Bax attenuated in the model rats after GPR treatment, the Bcl-2/Bax ratio increased, showing insignificant difference when compared with the blank control group (P > 0.05).


CONCLUSIONS
GPR plays a significant role in regulating the mRNA expressions of Bcl-2 and Bax, therefore improving the hearing of SYD rats and protecting the structure and function of cochlea.
Li
Li
L
Clinical Department of Integrative Medicine, Shanxi Traditional Chinese Medine College, Taiyuan. lili306871@126.com
Wang
Jun-Feng
JF
Xiong
Da-Jing
DJ
D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006319:Hearing Loss, Sensorineural / Q000188:drug therapy / Q000378:metabolism; D007674:Kidney Diseases / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008517:Phytotherapy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016711:Yang Deficiency / Q000188:drug therapy* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
C494828:Bax protein, rat; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein