Ultrananocrystalline diamond thin films functionalized with therapeutically active collagen networks.
The fabrication of biologically amenable interfaces in medicine bridges translational technologies with their surrounding biological environment. Functionalized nanomaterials catalyze this coalescence through the creation of biomimetic and active substrates upon which a spectrum of therapeutic elements can be delivered to adherent cells to address biomolecular processes in cancer, inflammation, etc. Here, we demonstrate the robust functionalization of ultrananocrystalline diamond (UNCD) with type I collagen and dexamethasone (Dex), an anti-inflammatory drug, to fabricate a hybrid therapeutically active substrate for localized drug delivery. UNCD oxidation coupled with a pH-mediated collagen adsorption process generated a comprehensive interface between the two materials, and subsequent Dex integration, activity, and elution were confirmed through inflammatory gene expression assays. These studies confer a translational relevance to the biofunctionalized UNCD in its role as an active therapeutic network for potent regulation of cellular activity toward applications in nanomedicine.
Huang
Houjin
H
Chen
Mark
M
Bruno
Paola
P
Lam
Robert
R
Robinson
Erik
E
Gruen
Dieter
D
Ho
Dean
D
D000327:Adsorption; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D015195:Drug Design; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D018130:Diamond; D003094:Collagen