The mRNA distribution of C7orf24, a gamma-glutamyl cyclotransferase, in rat tissues.
The putative protein C7orf24 is encoded by Homo sapiens chromosome 7 open reading frame 24. C7orf24 was first identified as a 21-kDa cytochrome c-releasing factor detected in the cytosolic fraction of human leukemia U937 cells after treatment with geranylgeraniol. C7orf24 protein was recently identified as a gamma-glutamyl cyclotransferase, an enzyme in the gamma-glutamyl cycle. However, the exact localization of C7orf24 mRNA in normal tissues remains unknown. The present study examined the distribution pattern of C7orf24 mRNA in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization histochemistry. The RT-PCR experiments demonstrated that C7orf24 and a variant C7orf24 mRNA were expressed in various tissues. Quantitative RT-PCR analysis revealed significantly high levels of both C7orf24 mRNAs in the liver and kidney, compared with other tissues examined. In situ hybridization histochemistry localized C7orf24 mRNA to hepatocytes in the liver and renal tubules in the kidney. The present results thus implicated an important role for C7orf24 in liver and kidney.
Oda
Keisuke
K
Professor, Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu 520-2192, Japan. kinchan@belle.shiga-med.ac.jp.
Makino
Satoshi
S
Masuda
Chiaki
C
Yoshiki
Tatsuhiro
T
Kitamura
Yoshihisa
Y
Takata
Kazuyuki
K
Yanagisawa
Daijiro
D
Taniguchi
Takashi
T
Tooyama
Ikuo
I
D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D005722:gamma-Glutamylcyclotransferase / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D005722:gamma-Glutamylcyclotransferase
Proteomics Clin Appl. 2007 Feb;1(2):192-9
21136669
Annu Rev Biochem. 1983;52:711-60
6137189
Science. 1973 Apr 6;180(4081):33-9
4144403
Genetics. 1981 Sep;99(1):109-16
6122625
FASEB J. 1999 Jul;13(10):1169-83
10385608
Biochem Biophys Res Commun. 2006 Jul 28;346(2):454-60
16765912
Mol Aspects Med. 2009 Feb-Apr;30(1-2):29-41
18786561
BMC Bioinformatics. 2006 Apr 11;7:202
16608523
J Biol Chem. 2008 Aug 8;283(32):22031-42
18515354
BMC Bioinformatics. 2007 Jul 30;8:275
17663766
Biochem Pharmacol. 1984 Jun 1;33(11):1801-7
6145422
Pharmacol Rev. 1998 Mar;50(1):107-41
9549760
J Biol Chem. 1973 Apr 25;248(8):2836-44
4735346