title: Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats.
abstract: OBJECTIVE
  Evaluation of the Mean Arterial Pressure (MAP) and body temperature changes in a new model of liver ischemia-reperfusion applying topical Hypothermia and Ischemic Preconditioning (IPC).
  
  
  METHODS
  Rats (n= 32) were divided in 5 groups: Control (C), Normothermic Ischemia (NI), Ischemic Preconditioning (IPC), Hypothermia 26 degrees C plus IPC (H+IPC) and Hypothermia 26 degrees C (H). MAP and body temperature were recorded at 30 minutes intervals throughout the entire experiment. The study groups underwent 90 minutes partial hepatic ischemia followed by 120 minutes of reperfusion. The median and lateral left lobes were isolated and topical 26 degrees C hypothermia was induced by superfusion of cooled saline solution in H+IPC and H groups. A 10 minutes protocol of ischemia and reperfusion was applied in the IPC and H+IPC groups before the major ischemic insult.
  
  
  RESULTS
  There was no significant difference in MAP and body temperature means between the groups throughout the experiments.
  
  
  CONCLUSIONS
  The present model allows the induction of topical hepatic hypothermia associated or not to IPC. New studies to evaluate the possible synergistic effects of these tools can be reproduced without significant changes in macrohemodynamics and body temperature, or in other words, under stable conditions.
authors.affiliation: Department of Surgery, UFRGS, Brazil. tomgrez@uol.com.br
authors.forename: Tomaz de Jesus Maria
authors.initials: Tde J
authors.lastname: Grezzana Filho
authors.forename: Tais Burmann de
authors.initials: TB
authors.lastname: Mendonça
authors.forename: Gémerson
authors.initials: G
authors.lastname: Gabiatti
authors.forename: Cleber Dario Pinto
authors.initials: CD
authors.lastname: Kruel
authors.forename: Carlos Otávio
authors.initials: CO
authors.lastname: Corso
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D001831:Body Temperature / Q000502:physiology*; D004195:Disease Models, Animal; D007036:Hypothermia, Induced / Q000379:methods*; D007511:Ischemia / Q000503:physiopathology*; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply*; D008297:Male; D051381:Rats; D017208:Rats, Wistar