No change in skeletal muscle satellite cells in young and aging rat soleus muscle.
Satellite cells are muscle stem cells capable of replenishing or increasing myonuclear number. It is postulated that a reduction in satellite cells may contribute to age-related sarcopenia. Studies investigating an age-related decline in satellite cells have produced equivocal results. This study compared the satellite cell content of young and aging soleus muscle in rat, using four different methods: dystrophin-laminin immunohistochemistry, MyoD immunohistochemistry, electron microscopy, and light microscopy of semi-thin sections. The absolute quantity of satellite cells increase with age, but satellite cell percentages were similar in young and aging soleus muscles. There were no differences in satellite cell quantity among MyoD immunohistochemistry, electron microscopy, and semi-thin sections. All three methods had significantly more satellite cells than with dystrophin-laminin immunohistochemistry. We conclude that satellite cell number does not decrease with age and postulate that satellite cell functionality may be responsible for age-related sarcopenia.
Brooks
Naomi E
NE
Department of Physiological Sciences, Stellenbosch University, Private Bag XI, Matieland, Stellenbosch, 7602, South Africa. nbrooks@sun.ac.za
Schuenke
Mark D
MD
Hikida
Robert S
RS
D000375:Aging / Q000502:physiology*; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology* / Q000648:ultrastructure; D009929:Organ Size; D051381:Rats; D011916:Rats, Inbred F344; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
Anat Rec. 1971 Aug;170(4):421-35
5118594
Am J Anat. 1976 Sep;147(1):49-70
970346
Mech Ageing Dev. 1982 Dec;20(4):377-83
7166986
Am J Physiol. 1997 Aug;273(2 Pt 1):C579-87
9277355
Semin Cell Dev Biol. 2005 Aug-Oct;16(4-5):612-22
16087370
J Appl Physiol (1985). 2004 Sep;97(3):1082-90
15121742
Am J Physiol Cell Physiol. 2002 Aug;283(2):C484-8
12107058
Muscle Nerve. 1994 Jun;17(6):608-13
8196703
Anat Rec. 1997 Mar;247(3):350-4
9066912
Anat Rec. 1978 Apr;190(4):891-903
637326
N Engl J Med. 1994 Jun 23;330(25):1769-75
8190152
J Cell Sci. 2008 Sep 15;121(Pt 18):2975-82
18768931
Am J Physiol. 1997 Feb;272(2 Pt 1):C565-71
9124300
J Appl Physiol (1985). 1999 Nov;87(5):1776-85
10562622
Eur J Clin Nutr. 2000 Jun;54 Suppl 3:S15-20
11041070
Muscle Nerve. 2004 Jan;29(1):120-7
14694507
Am J Physiol Cell Physiol. 2001 Nov;281(5):C1706-15
11600435
J Appl Physiol (1985). 2004 Nov;97(5):1715-22
15234958
Stem Cells. 2007 Apr;25(4):885-94
17218401
J Histochem Cytochem. 2003 Jul;51(7):853-63
12810836
Acta Physiol (Oxf). 2009 Sep;197(1):55-63
19254285
Histochem Cell Biol. 2000 Feb;113(2):99-103
10766262
Muscle Nerve. 1983 Oct;6(8):574-80
6646160
Muscle Nerve. 1999 Oct;22(10):1350-60
10487900
J Appl Physiol (1985). 1996 Jul;81(1):145-51
8828656
Scand J Med Sci Sports. 1995 Jun;5(3):129-42
7552755
Cell Tissue Res. 1977 Dec 19;185(3):399-408
597854
Neuromuscul Disord. 2005 Jan;15(1):57-64
15639122
Exerc Sport Sci Rev. 2008 Apr;36(2):51-7
18362685
Dev Biol. 1986 May;115(1):140-7
3699242
J Physiol. 2004 Aug 1;558(Pt 3):1005-12
15218062
J Appl Physiol (1985). 1998 Apr;84(4):1407-12
9516210
J Gerontol A Biol Sci Med Sci. 2000 Jul;55(7):B347-54
10898248
Muscle Nerve. 1998 Feb;21(2):173-83
9466592
Dev Biol. 2006 Jun 1;294(1):50-66
16554047
J Appl Physiol (1985). 2001 Aug;91(2):534-51
11457764
Anat Rec. 2000 Dec 1;260(4):351-8
11074399
J Cell Biol. 2000 Dec 11;151(6):1221-34
11121437
Science. 2003 Nov 28;302(5650):1575-7
14645852
Cell. 2003 May 16;113(4):422-3
12757701
Nature. 1989 Feb 9;337(6207):570-3
2915707
Exp Gerontol. 2002 Dec;37(12):1513-4
12559420
Nature. 2005 Feb 17;433(7027):760-4
15716955
Cell. 1989 Dec 1;59(5):771-2
2686838
Biogerontology. 2005;6(5):335-43
16463110
J Histochem Cytochem. 2004 Feb;52(2):179-85
14729869