An amphipathic alpha-helix at the C terminus of hepatitis C virus nonstructural protein 4B mediates membrane association.
Nonstructural protein 4B (NS4B) plays an essential role in the formation of the hepatitis C virus (HCV) replication complex. It is an integral membrane protein that has been only poorly characterized to date. It is believed to comprise a cytosolic N-terminal part, a central part harboring four transmembrane passages, and a cytosolic C-terminal part. Here, we describe an amphipathic alpha-helix at the C terminus of NS4B (amino acid residues 229 to 253) that mediates membrane association and is involved in the formation of a functional HCV replication complex.
Gouttenoire
Jérôme
J
Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, BU44/07/2421, Rue du Bugnon 44, CH-1011 Lausanne, Switzerland.
Montserret
Roland
R
Kennel
Audrey
A
Penin
François
F
Moradpour
Darius
D
D000595:Amino Acid Sequence; D002460:Cell Line; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053586:Virus Internalization; D014779:Virus Replication
D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins
Biochemistry. 2009 Feb 10;48(5):906-16
19146391
J Virol. 2003 May;77(9):5428-38
12692244
J Virol. 2004 Dec;78(23):13278-84
15542678
J Gen Virol. 2006 Nov;87(Pt 11):3263-3272
17030859
J Virol. 2004 Oct;78(20):11393-400
15452261
Hepatology. 2005 Oct;42(4):962-73
16149085
J Virol. 2004 Oct;78(20):11288-95
15452248
Hepatology. 1998 Jul;28(1):192-201
9657112
Nat Struct Biol. 1996 Oct;3(10):842-8
8836100
J Virol. 2001 Nov;75(22):10651-62
11602707
J Gen Virol. 2005 May;86(Pt 5):1415-1421
15831953
J Virol. 2003 May;77(9):5487-92
12692249
J Biol Chem. 2002 Mar 8;277(10):8130-9
11744739
J Biomol NMR. 1999 Mar;13(3):289-302
10212987
J Mol Graph. 1996 Feb;14(1):33-8, 27-8
8744570
Nat Struct Mol Biol. 2007 Feb;14(2):138-46
17220896
Trends Biochem Sci. 2000 Mar;25(3):147-50
10694887
Science. 1997 Jul 25;277(5325):570-4
9228008
J Virol. 2002 Jun;76(12):5974-84
12021330
J Biomol NMR. 1995 Jan;5(1):14-24
7881270
J Biol Chem. 2004 Sep 24;279(39):40835-43
15247283
Nat Rev Microbiol. 2007 Jun;5(6):453-63
17487147
J Virol. 2009 Jun;83(12):6257-68
19357161
J Mol Biol. 1997 Jul 18;270(3):496-510
9237914
J Virol. 2007 May;81(10):5270-83
17344294
Nat Biotechnol. 2008 Sep;26(9):1019-27
18758449
Biochemistry. 1998 Oct 20;37(42):14713-8
9778346
Virol J. 2009 May 25;6:62
19467155
Virology. 2001 May 25;284(1):70-81
11352669
J Virol. 2006 Jun;80(12):6013-23
16731940
J Magn Reson. 2003 Jan;160(1):65-73
12565051
Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14545-50
18799730
J Biol Chem. 2001 Nov 23;276(47):44052-63
11557752
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417