[Expression of tissue transglutaminase on renal interstitial fibrosis rats and intervention of GBE].
OBJECTIVE
To investigate the expression of tissue transglutaminase( tTG) in unilateral ureteral obstruction (UUO) rats and the intervention of Folium Ginkgo (GBE).


METHODS
The animal models of unilateral ureteral obstruction (UUO) were used. Wistar rats were randomly divided into four groups, the Sham-operated group, the UUO group, the benazepril (Bena)-treated UUO group and the GBE-treated group. The rats were sacrificed at day 14. Histological changes in renal tubular interstitium were observed with HE and Masson staining, and the mRNA and protein levels of tTG and FN were detected by RT-PCR and immunohistochemistry.


RESULTS
Compared with the sham group, the expression of tTG and FN were significantly increased (P < 0.01), and decreased after been treated by Bena and GBE (P < 0.05).


CONCLUSIONS
Bena and GBE may suppress the development of fibrosis partially via down-regulation of tTG expression.
Wang
Dan
D
Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, Changchun 130021, China.
Li
Xiangjun
X
Yu
Xiaoyan
X
Shi
Yan
Y
Yin
Li
L
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005355:Fibrosis; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D020441:Ginkgo biloba / Q000737:chemistry*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011503:Transglutaminases / Q000235:genetics* / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D011503:Transglutaminases