Hemostatic efficacy of biological self-assembling peptide nanofibers in a rat kidney model.
We evaluated the hemostatic efficacy of a biological self-assembling peptide RADA16-I in a rat kidney injury model. Adult male rats were randomized into five groups: sham operation (no renal excision), no hemostatic agent (control), commercially available gelatin sponge (Gelfoam), 1% RADA16-I, and 2% RADA16-I. After left partial nephrectomy, the anesthetized animal was anticoagulated using 300 IU x kg(-1) heparin, and the topical hemostatic agent was applied to the injury. Blood loss and mean arterial pressure (MAP) were recorded. As was the case for Gelfoam, 2% RADA16-I produced marked hemostasis versus controls (p < 0.01). Blood loss with 1% and 2% RADA16-I was significantly less than controls. The decline in MAP during surgery was less with 2% versus 1% RADA16-I. RADA16-I also resulted in less histological tissue responses than Gelfoam. These data suggest that RADA16-I can stop hemorrhage, with only minimal tissue responses, in experimental renal injury.
Song
Hong
H
Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Science Park No. 1, Ke Yuan 4th Street, Gao Peng Road, Hi-tech Industrial Development Zone, Chengdu, 610041, China.
Zhang
Lanlan
L
Zhao
Xiaojun
X
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure; D004305:Dose-Response Relationship, Drug; D006470:Hemorrhage / Q000188:drug therapy*; D006490:Hemostatics / Q000627:therapeutic use*; D007668:Kidney / Q000473:pathology / Q000601:surgery*; D008297:Male; D018625:Microscopy, Atomic Force; D057139:Nanofibers / Q000627:therapeutic use*; D009392:Nephrectomy; D010455:Peptides / Q000627:therapeutic use*; D019106:Postoperative Hemorrhage; D051381:Rats
D006490:Hemostatics; D010455:Peptides; C546681:RADA16-I