Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales.
A 3-hydroxypropionate/4-hydroxybutyrate cycle operates during autotrophic CO(2) fixation in various members of the Crenarchaea. In this cycle, as determined using Metallosphaera sedula, malonyl-coenzyme A (malonyl-CoA) and succinyl-CoA are reductively converted via their semialdehydes to the corresponding alcohols 3-hydroxypropionate and 4-hydroxybutyrate. Here three missing oxidoreductases of this cycle were purified from M. sedula and studied. Malonic semialdehyde reductase, a member of the 3-hydroxyacyl-CoA dehydrogenase family, reduces malonic semialdehyde with NADPH to 3-hydroxypropionate. The latter compound is converted via propionyl-CoA to succinyl-CoA. Succinyl-CoA reduction to succinic semialdehyde is catalyzed by malonyl-CoA/succinyl-CoA reductase, a promiscuous NADPH-dependent enzyme that is a paralogue of aspartate semialdehyde dehydrogenase. Succinic semialdehyde is then reduced with NADPH to 4-hydroxybutyrate by succinic semialdehyde reductase, an enzyme belonging to the Zn-dependent alcohol dehydrogenase family. Genes highly similar to the Metallosphaera genes were found in other members of the Sulfolobales. Only distantly related genes were found in the genomes of autotrophic marine Crenarchaeota that may use a similar cycle in autotrophic carbon fixation.
Kockelkorn
Daniel
D
Mikrobiologie, Fakultät Biologie, Schänzlestr. 1, D-79104 Freiburg, Germany.
Fuchs
Georg
G
D000214:Acyl Coenzyme A / Q000378:metabolism*; D019843:Archaeal Proteins / Q000378:metabolism*; D052818:Autotrophic Processes; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D019344:Lactic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D008315:Malondialdehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D011994:Recombinant Proteins; D016942:Sulfolobales / Q000201:enzymology* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000378:metabolism
D000214:Acyl Coenzyme A; D019843:Archaeal Proteins; D006885:Hydroxybutyrates; D011994:Recombinant Proteins; C111420:4-hydroxybutyric acid; D019344:Lactic Acid; D008315:Malondialdehyde; D005680:gamma-Aminobutyric Acid; C039786:malonic semialdehyde; C012046:succinyl-coenzyme A; C031601:hydracrylic acid; D010088:Oxidoreductases; C009338:succinic semialdehyde
Eur J Biochem. 2003 Feb;270(4):736-44
12581213
Methods Enzymol. 1982;87:405-26
7176924
Nature. 1970 Aug 15;227(5259):680-5
5432063
Anal Biochem. 1976 May 7;72:248-54
942051
Biochemistry. 1990 Jun 19;29(24):5647-59
2200508
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8
3156376
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Arch Microbiol. 2003 Mar;179(3):160-73
12610721
Biochemistry. 1966 Feb;5(2):467-77
5942950
J Bacteriol. 2009 Jul;191(14):4572-81
19429610
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Methods Enzymol. 1972;24:53-68
4206745
Eur J Biochem. 1992 Apr 15;205(2):853-66
1572376
J Bacteriol. 2006 Dec;188(24):8551-9
17041055
Cell Mol Life Sci. 2008 Dec;65(24):3879-94
19011751
J Biol Chem. 2002 Jun 7;277(23):20277-83
11929869
Anal Biochem. 1989 Oct;182(1):157-9
2481413
J Bacteriol. 1999 Feb;181(4):1088-98
9973333
J Bacteriol. 2008 Feb;190(4):1383-9
18165310
J Bacteriol. 2008 Feb;190(4):1366-74
18065535
Comput Appl Biosci. 1994 Sep;10(5):569-70
7828077
Biochem J. 1992 Oct 15;287 ( Pt 2):361-5
1445195
J Bacteriol. 2003 Feb;185(3):938-47
12533469
J Bacteriol. 2001 Jul;183(14):4305-16
11418572
Anal Biochem. 1980 May 15;104(2):300-10
7446957
Eur J Biochem. 1993 Aug 1;215(3):633-43
8354269
J Bacteriol. 2009 Jul;191(13):4286-97
19411323
Cell Mol Life Sci. 2008 Dec;65(24):3961-70
19011745
Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7851-6
18511565
Protein Expr Purif. 1995 Apr;6(2):206-12
7606170
J Bacteriol. 2002 May;184(9):2404-10
11948153
J Bacteriol. 2007 Apr;189(7):2906-14
17259315
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D16-20
16381837
Environ Microbiol. 2007 Aug;9(8):2067-78
17635550
Appl Environ Microbiol. 2008 Feb;74(3):682-92
18083856
Arch Microbiol. 1996 Dec;166(6):368-71
9082912
J Biol Chem. 2003 Oct 17;278(42):41552-6
12882961
Gene. 1988 Sep 30;69(2):301-15
3069586
J Biol Chem. 2002 Apr 5;277(14):12137-43
11821399
Eur J Biochem. 1993 Aug 1;215(3):619-32
8354268
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
Science. 2007 Dec 14;318(5857):1782-6
18079405
J Biol Chem. 1959 Jul;234(7):1666-71
13672942