Nonequilibrium separation of short DNA using nanoslit arrays.
A nonequilibrium regime of size-based separation was observed experimentally for double-stranded deoxyribonucleic acid (DNA) molecules with lengths below 1 kbp moving electrokinetically through nanofluidic nanoslit arrays. The breakdown of Ogston sieving was supplanted at higher electric fields to recover rapid separation with a reversed elution order and elution times one to two orders of magnitude faster than with Ogston sieving at lower fields. A simple kinetic model describes the experimental results.
Strychalski
Elizabeth A
EA
Lau
Henry W
HW
Archer
Lynden A
LA
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