Construction and expression of a recombinant fibronectinIII10 protein for integrin-mediated cell adhesion.
Specific sequences of cell-adhesive peptide/proteins are often required for the bio-inert synthetic polymers to improve cell adhesion. We have developed a recombinant fibronectin fragment (FN(III)10), the central cell-binding domain containing RGD motif, to endow biomaterial surfaces with abilities to promote an integrin-mediated cell adhesion. Immobilized FN(III)10 stimulated adhesion of MC3TC-E1 cells in an integrin-dependent manner.
Kim
Hae-Won
HW
Department of Biomaterials Science, School of Dentistry and Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Seoul, Korea.
Kang
Wonmo
W
Jeon
Eunyi
E
Jang
Jun-Hyeog
JH
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D016023:Integrins / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D009842:Oligopeptides / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D011994:Recombinant Proteins; C049554:glycyl-arginyl-glycyl-aspartyl-serine