A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction.
Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis.
Couto
Manuela Soares
MS
Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará CEP: 60.425-540, Brazil.
Cordeiro
Rossana de Aguiar
Rde A
Rocha
Marcos Fábio Gadelha
MF
Grangeiro
Thalles Barbosa
TB
Leitão Junior
Natanael Pinheiro
NP
Bandeira
Tereza de Jesus Pinheiro Gomes
Tde J
Sidrim
José Júlio Costa
JJ
Brilhante
Raimunda Sâmia Nogueira
RS
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014481:United States
D017931:DNA Primers; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer