title: What Makes each Aux/IAA Gene Unique in its Gene Family, Expression Pattern or Properties of the Gene Product?
abstract: In the auxin signal transduction, two protein families, Aux/IAAs and auxin response factors, play a crucial role just downstream of auxin F-box receptors. Distinct and overlapping phenotypes of the dominant Aux/IAA mutants suggest some functional differentiation of the Aux/IAA genes in auxin signaling. Taking advantage of unique phenotypes of the msg2/iaa19 mutants, we carried out promoter-exchange experiments, where cDNA of the msg2, axr2/iaa7 or slr/iaa14 gene was driven by the MSG2 or AXR2 promoter. The cDNAs were translationally fused to the green fluorescent protein gene to measure levels of expressed protein. Results showed that many abnormal phenotypes of the dominant Aux/IAA mutants were governed by their promoter activity, but some were dependent on their gene products. The latter result highlights the possible importance of Aux/IAA protein level controled by auxin F-box receptors.
authors.affiliation: Department of Biological Sciences; Faculty of Science; Hokkaido University; Sapporo Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Muto
authors.forename: Masaaki K
authors.initials: MK
authors.lastname: Watahiki
authors.forename: Kotaro T
authors.initials: KT
authors.lastname: Yamamoto
references.citation: Plant Physiol. 2000 Jun;123(2):563-74
references.pmid: 10859186
references.citation: Plant Physiol. 1997 Oct;115(2):419-26
references.pmid: 9342863
references.citation: Plant Cell. 2001 Apr;13(4):829-41
references.pmid: 11283339
references.citation: Plant Cell. 2004 Feb;16(2):379-93
references.pmid: 14729917
references.citation: Plant Cell. 2005 Feb;17(2):444-63
references.pmid: 15659631
references.citation: Development. 2003 Dec;130(23):5769-77
references.pmid: 14534134
references.citation: EMBO J. 2005 May 18;24(10):1874-85
references.pmid: 15889151
references.citation: Plant Physiol. 2007 May;144(1):187-96
references.pmid: 17369427
references.citation: Plant Cell Physiol. 2006 Aug;47(8):1095-101
references.pmid: 16854942
references.citation: Plant J. 2005 Jul;43(1):118-30
references.pmid: 15960621
references.citation: Development. 2005 Sep;132(18):4107-18
references.pmid: 16107481
references.citation: Plant J. 2005 Nov;44(3):382-95
references.pmid: 16236149
references.citation: Nature. 2007 Apr 5;446(7136):640-5
references.pmid: 17410169
references.citation: Genes Dev. 2002 Jul 1;16(13):1610-5
references.pmid: 12101120
references.citation: Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11786-91
references.pmid: 9342315
references.citation: Development. 2004 Mar;131(5):1089-100
references.pmid: 14973283
references.citation: Nature. 2005 May 26;435(7041):446-51
references.pmid: 15917798
references.citation: Plant Cell. 2002 Feb;14(2):301-19
references.pmid: 11884676
references.citation: Nature. 2005 May 26;435(7041):441-5
references.pmid: 15917797
references.citation: Science. 1997 Jun 20;276(5320):1865-8
references.pmid: 9188533