Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells.
The effect of Tunisian Capparis spinosa L. aromatic plant extract on melanogenesis regulation in B16 murine melanoma cells was investigated. B16 cells were treated with 0.0005, 0.005, and 0.05% (w/v) C. spinosa extract after which the melanin content and cell viability were measured. To clarify the mechanism behind melanogenesis regulation, the expression of tyrosinase was determined. Results showed that the extract had a significant stimulative effect on melanogenesis in B16 cells in a dose-dependent manner without cytotoxicity. Western blot analysis showed that expression of tyrosinase in cells treated with 0.03% (w/v) C. spinosa extract increased by 12.5- and 20-fold after 24 and 48 h of incubation, respectively, compared with untreated cells. HPLC analysis of the extract revealed the presence of 1% quercetin, a known melanogenesis stimulator, indicating that our findings may be attributed to quercetin; however, other compounds present in the extract may also have an effect on the overall ability of the extract to stimulate melanogenesis. We report here that Tunisian C. spinosa leaf extract can stimulate melanogenesis in a dose-dependent manner without cytotoxicity by increasing tyrosinase protein expression and has the potential to be used as a possible tanning agent or as a treatment for hair depigmentation.
Matsuyama
Kyoko
K
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
Villareal
Myra O
MO
El Omri
Abdelfatteh
A
Han
Junkyu
J
Kchouk
Mohamed Elyes
ME
Isoda
Hiroko
H
D000818:Animals; D015153:Blotting, Western; D031484:Capparis / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D014416:Tunisia
D008543:Melanins; D010936:Plant Extracts; D014442:Monophenol Monooxygenase
Phytochemistry. 2000 Nov;55(6):481-504
11130659
Biosci Biotechnol Biochem. 2005 May;69(5):873-82
15914904
Pigment Cell Res. 2004 Feb;17(1):66-73
14717847
Pigment Cell Res. 2001 Apr;14(2):132-9
11310793
Pigment Cell Res. 1995 Apr;8(2):97-104
7659683
Phytother Res. 2008 Jul;22(7):867-72
18567053
Acta Pharmacol Sin. 2006 Nov;27(11):1467-73
17049123
Phytother Res. 2006 Sep;20(9):748-54
16807878
J Mol Histol. 2004 Feb;35(2):157-65
15328920
Biochem Biophys Res Commun. 1999 Mar 24;256(3):657-63
10080955
Biochem Pharmacol. 1992 Mar 17;43(6):1167-79
1562270
Fitoterapia. 2000 Feb;71(1):46-9
11449469
Pigment Cell Res. 2005 Dec;18(6):439-46
16280009
News Physiol Sci. 2000 Feb;15:11-15
11390869
Exp Mol Med. 2001 Sep 30;33(3):131-5
11642548
Dermatology. 2001;202(1):22-6
11244223
Cancer Res. 1985 Apr;45(4):1474-8
2983883
Am J Pathol. 2003 Jul;163(1):333-43
12819038
Biochem J. 1998 Mar 15;330 ( Pt 3):1235-9
9494091