Effects of platelet-rich plasma gel on skin healing in surgical wound in horses.
OBJECTIVE
To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine.


METHODS
To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair.


RESULTS
The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation.


CONCLUSIONS
Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine.
DeRossi
Rafael
R
Department of Veterinary Medicine, Surgery and Anesthesiology, Faculty of Veterinary Medicine and Animal Science, UFMS, Mato Grosso do Sul, Brazil. derossi@nin.ufms.br
Coelho
Anna Carolina Anciliero de Oliveira
AC
Mello
Gisele Silveira de
GS
Frazílio
Fabrício Oliveira
FO
Leal
Cássia Rejane Brito
CR
Facco
Gilberto Gonçalves
GG
Brum
Karine Bonucielli
KB
D000818:Animals; D001706:Biopsy; D002125:Calcium Gluconate / Q000627:therapeutic use*; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D005260:Female; D005782:Gels; D006490:Hemostatics / Q000627:therapeutic use*; D006736:Horses; D008297:Male; D053657:Platelet-Rich Plasma; D012867:Skin / Q000473:pathology / Q000503:physiopathology; D013917:Thrombin / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014947:Wounds and Injuries / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*
D005782:Gels; D006490:Hemostatics; D013917:Thrombin; D002125:Calcium Gluconate