Studies on the mechanism of resistance to Bipolaris sorokiniana in the barley lesion mimic mutant bst1.
SUMMARY The Bipolaris sorokiniana tolerant 1 (bst1) barley mutant is derived from fast neutron-irradiated seeds of wild-type Bowman(Rph3). The induced mutation was genetically localized to a position on chromosome 5HL distal to the centromere using amplified fragment length polymorphism markers. In addition, the defence responses and related gene expression in the bst1 mutant after fungal challenge were compared with those occurring in wild-type plants. Hydrogen peroxide generation, determined by 3,3-diaminobenzidine staining, revealed a clearly reduced level of bst1, compared with the wild-type, during the entire experimental time: 8-120 h post-inoculation (hpi). At 48 hpi, the wild-type samples displayed twice as much fungal mass and three times greater H(2)O(2) production than bst1. At the same time, staining of B. sorokiniana showed less fungal growth in the spontaneous lesions of bst1 compared with the wild-type. Monitoring of defence-related genes at 48 hpi demonstrated strong expression of PR-1a, PR-2, PR-5 and PR-10 in bst1. A gene coding for a unique oxidoreductase enzyme, designated as HCP1, was expressed at much higher levels in inoculated leaves of the bst1 mutant than in those of the wild-type plant. Taken together, the results suggest that the defence to B. sorokiniana largely relies on salicylic acid-responsive pathogenesis-related (PR) genes, as well as selected reactive oxygen species and unknown HCP1-associated factors.
Persson
Mattias
M
Department of Plant Biology and Forest Genetics, Uppsala BioCenter, SLU, PO Box 7080, 750 07 Uppsala, Sweden. mattias.persson@vbsg.slu.se
Falk
Anders
A
Dixelius
Christina
C
D001203:Ascomycota / Q000502:physiology*; D016923:Cell Death / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008040:Genetic Linkage; D001467:Hordeum / Q000166:cytology / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D006861:Hydrogen Peroxide
Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10870-5
12122207
Nucleic Acids Res. 1995 Nov 11;23(21):4407-14
7501463
J Exp Bot. 2006;57(10):2277-89
16831849
Plant J. 2001 Mar;25(5):563-74
11309146
Plant Physiol. 2006 Jun;141(2):404-11
16603664
Curr Opin Plant Biol. 2007 Aug;10(4):372-9
17646123
J Cell Biol. 2005 Jan 3;168(1):17-20
15631987
Plant J. 2001 May;26(4):447-59
11439131
Nat Biotechnol. 2000 Jul;18(7):779-83
10888849
Plant J. 2002 Feb;29(4):427-37
11846876
Plant Physiol. 2007 Mar;143(3):1282-92
17277089
Mol Plant Pathol. 2002 Jul 1;3(4):185-95
20569326
J Exp Bot. 2007;58(12):3197-212
17761729
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9323-8
10900264
Annu Rev Phytopathol. 2004;42:385-414
15283671
Plant J. 1999 Mar;17(5):535-45
10205906
Plant Cell. 2002 Dec;14(12):3149-62
12468733
Anal Biochem. 1976 May 7;72:248-54
942051
Trends Plant Sci. 2003 Jun;8(6):263-71
12818660
Annu Rev Phytopathol. 2005;43:205-27
16078883
Mol Plant Microbe Interact. 2007 Apr;20(4):335-45
17427804
Bioessays. 2006 Nov;28(11):1091-101
17041898
Plant J. 2002 May;30(4):467-80
12028576
Annu Rev Plant Biol. 2008;59:21-39
18031216
Mol Genet Genomics. 2006 Feb;275(2):159-68
16341885
Eur J Biochem. 1997 Nov 1;249(3):748-57
9395322
Nat Genet. 2005 Oct;37(10):1130-4
16170317
Mol Plant Microbe Interact. 2003 Apr;16(4):352-9
12744464
Plant J. 2008 Jun;54(5):785-93
18248595
Plant Physiol. 2002 Jul;129(3):1076-85
12114562
Plant Cell. 1996 Oct;8(10):1793-1807
12239362
Mol Gen Genet. 1993 May;239(1-2):122-8
8510641
Cell. 2006 Feb 24;124(4):803-14
16497589
Proteomics. 2008 Feb;8(4):893-908
18210371
FEBS Lett. 2006 Jun 12;580(14):3498-504
16730712
Plant Cell. 2004 Aug;16(8):2217-32
15269331
EMBO J. 1987 Dec 20;6(13):3901-7
3327686
Plant Physiol. 2007 Oct;145(2):465-77
17720753
Cell. 1994 May 20;77(4):565-77
8187176
Nature. 2006 Nov 16;444(7117):323-9
17108957
Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7819-24
9636234
Mol Plant Microbe Interact. 2000 Aug;13(8):869-76
10939258
Plant Cell. 1994 Nov;6(11):1583-1592
12244227
Protoplasma. 2003 May;221(1-2):137-43
12768351
Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):364-9
12509512
Plant Cell. 2001 Oct;13(10):2211-24
11595797
Cell. 1997 Mar 7;88(5):695-705
9054509
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828-32
1682921
Plant J. 2006 Aug;47(4):532-46
16813576
Genetics. 1998 Aug;149(4):2039-56
9691056
Mol Plant Pathol. 2008 Jan;9(1):45-57
18705883
Theor Appl Genet. 2003 Oct;107(6):1094-101
12928776
Plant Cell. 1990 May;2(5):437-45
2152169