title: Medial auditory thalamic input to the lateral pontine nuclei is necessary for auditory eyeblink conditioning.
abstract: Auditory and visual conditioned stimulus (CS) pathways for eyeblink conditioning were investigated with reversible inactivation of the medial (MPN) or lateral (LPN) pontine nuclei. In Experiment 1, Long-Evans rats were given three phases of eyeblink conditioning. Phase 1 consisted of three training sessions with electrical stimulation of the medial auditory thalamic nuclei (MATN) paired with a periorbital shock unconditioned stimulus (US). An additional session was given with a muscimol (0.5muL, 10mM) or saline infusion targeting the LPN followed by a recovery session with no infusions. The same training and testing sequence was then repeated with either a tone or light CS in phases 2 and 3 (counterbalanced). Experiment 2 consisted of the same training as Experiment 1 except that muscimol or saline was infused in the MPN during the retention tests. Muscimol infusions targeting the LPN severely impaired retention of eyeblink conditioned responses (CRs) to the MATN stimulation and tone CSs but only partially reduced CR percentage to the light CS. Muscimol infusions that targeted the MPN had a larger effect on CR retention to the light CS relative to MATN stimulation or tone CSs. The results provide evidence that the auditory CS pathway necessary for delay eyeblink conditioning includes the MATN-LPN projection and the visual CS pathway includes the MPN.
authors.affiliation: Department of Psychology, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Hunter E
authors.initials: HE
authors.lastname: Halverson
authors.forename: John H
authors.initials: JH
authors.lastname: Freeman
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001306:Auditory Pathways / Q000502:physiology*; D001307:Auditory Perception / Q000502:physiology*; D002404:Catheterization; D003215:Conditioning, Eyelid / Q000502:physiology*; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004597:Electroshock; D018755:GABA Agonists / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D008568:Memory / Q000502:physiology*; D008839:Microelectrodes; D009118:Muscimol / Q000008:administration & dosage / Q000494:pharmacology; D011149:Pons / Q000502:physiology*; D051381:Rats; D020318:Rats, Long-Evans; D013787:Thalamic Nuclei / Q000502:physiology*
chemical_list: D018755:GABA Agonists; D009118:Muscimol
references.citation: Learn Mem. 1997 Mar-Apr;3(6):519-31
references.pmid: 10456114
references.citation: Behav Neurosci. 1992 Jun;106(3):509-17
references.pmid: 1616617
references.citation: Science. 1984 Jan 20;223(4633):296-9
references.pmid: 6701513
references.citation: Neuron. 1999 Sep;24(1):179-85
references.pmid: 10677036
references.citation: J Neurosci. 2007 Mar 7;27(10):2493-502
references.pmid: 17344387
references.citation: Physiol Behav. 1972 May;8(5):915-26
references.pmid: 5046247
references.citation: Behav Brain Res. 1993 Jul 30;56(1):23-30
references.pmid: 7691076
references.citation: Learn Mem. 2005 May-Jun;12(3):255-9
references.pmid: 15930504
references.citation: Behav Neural Biol. 1994 May;61(3):218-24
references.pmid: 8067977
references.citation: Hear Res. 1978 Oct;1(1):67-77
references.pmid: 757232
references.citation: Learn Mem. 2003 Sep-Oct;10(5):346-54
references.pmid: 14557607
references.citation: Neuropharmacology. 1998 Apr-May;37(4-5):471-80
references.pmid: 9704988
references.citation: Behav Neural Biol. 1992 Mar;57(2):103-15
references.pmid: 1586349
references.citation: Integr Physiol Behav Sci. 2004 Jul-Sep;39(3):180-91
references.pmid: 15929500
references.citation: Learn Mem. 1997 Mar-Apr;3(6):545-56
references.pmid: 10456116
references.citation: J Neurosci. 2005 Jan 26;25(4):889-95
references.pmid: 15673669
references.citation: J Neurosci. 1988 Feb;8(2):417-27
references.pmid: 3339424
references.citation: Behav Brain Res. 1999 Nov 15;105(2):189-98
references.pmid: 10563492
references.citation: Behav Neurosci. 1986 Dec;100(6):878-87
references.pmid: 3814342
references.citation: Physiol Behav. 1988;43(6):855-7
references.pmid: 3237801
references.citation: Neurosci Lett. 1991 Jun 24;127(2):160-4
references.pmid: 1881625
references.citation: Physiol Behav. 1971 Apr;6(4):273-8
references.pmid: 5148739
references.citation: Behav Neurosci. 2009 Apr;123(2):292-302
references.pmid: 19331453
references.citation: Behav Neurosci. 2000 Apr;114(2):254-61
references.pmid: 10832787
references.citation: Behav Neurosci. 2003 Apr;117(2):292-303
references.pmid: 12708526
references.citation: Proc Natl Acad Sci U S A. 1986 Jul;83(14):5349-53
references.pmid: 3460097
references.citation: Physiol Behav. 1977 May;18(5):931-7
references.pmid: 905402
references.citation: Learn Mem. 2008 Jul 11;15(7):532-8
references.pmid: 18626096
references.citation: Proc Natl Acad Sci U S A. 1982 Apr;79(8):2731-5
references.pmid: 6953427
references.citation: Learn Mem. 2007 Dec 17;14(12):842-6
references.pmid: 18086827
references.citation: Annu Rev Psychol. 2005;56:1-23
references.pmid: 15709927
references.citation: Am Psychol. 1976 Mar;31(3):209-27
references.pmid: 766675
references.citation: Science. 1993 May 14;260(5110):989-91
references.pmid: 8493536
references.citation: Behav Neurosci. 1998 Apr;112(2):267-85
references.pmid: 9588477
references.citation: J Neurosci Methods. 1980 Dec;3(2):129-49
references.pmid: 6110810
references.citation: Learn Behav. 2008 May;36(2):159-65
references.pmid: 18543716
references.citation: Learn Mem. 2003 Nov-Dec;10(6):427-55
references.pmid: 14657256
references.citation: Learn Mem. 2003 Sep-Oct;10(5):337-45
references.pmid: 14557606
references.citation: Brain Res. 1985 Dec 16;359(1-2):120-30
references.pmid: 4075140
references.citation: Brain Res. 1975 Sep 23;95(2-3):309-22
references.pmid: 1156878
references.citation: J Neurosci. 2001 Oct 1;21(19):7715-23
references.pmid: 11567061
references.citation: J Exp Psychol Anim Behav Process. 1991 Jan;17(1):26-35
references.pmid: 2002305
references.citation: Learn Mem. 2007 Mar 08;14(3):152-9
references.pmid: 17351138
references.citation: Behav Neurosci. 2006 Aug;120(4):880-7
references.pmid: 16893294
references.citation: Learn Mem. 1997 Mar-Apr;3(6):532-44
references.pmid: 10456115
references.citation: Behav Neurosci. 2002 Feb;116(1):22-36
references.pmid: 11895180
references.citation: Brain Res. 1987 Jun 9;413(1):170-4
references.pmid: 3594255
references.citation: J Comp Neurol. 1989 Jan 22;279(4):629-52
references.pmid: 2465324
references.citation: Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):8120-3
references.pmid: 18523018
references.citation: J Neurosci. 1984 Nov;4(11):2811-22
references.pmid: 6502205
references.citation: Exp Brain Res. 1974;20(3):303-6
references.pmid: 4426354
references.citation: Proc Natl Acad Sci U S A. 1987 May;84(10):3531-5
references.pmid: 3554242
references.citation: Behav Neurosci. 1987 Apr;101(2):151-7
references.pmid: 3580118