title: Revisiting delta-6 desaturase regulation by C18 unsaturated fatty acids, depending on the nutritional status.
abstract: Dietary polyunsaturated fatty acids (PUFA) play a key role in regulating delta-6 desaturase (D6D), the key enzyme for long-chain PUFA biosynthesis. Nevertheless, the extent of their effects on this enzyme remains controversial and difficult to assess. It has been generally admitted that C18 unsaturated fatty acids (UFAs) regulate negatively delta-6 desaturase (D6D). This inhibition has been evidenced in regard to a high glucose/fat free (HG/FF) diet used in reference. However, several nutritional investigations did not evidence any inhibition of desaturases when feeding fatty acids. Because the choice of the basal diet appeared to be of primary importance in such experiments, our goal was to reconsider the specific role of dietary UFAs on D6D regulation, depending on nutritional conditions. For that, sixteen adult Wistar rats were fed purified linoleic acid, alpha-linolenic acid or oleic acid, included in one of two diets at 4% by weight: an HG/FF or a high starch base (HS) where the pure UFAs replaced a mixed vegetable oil. Our results showed first that D6D specific activity was significantly greater when measured in presence of an HG/FF than with an HS/4% vegetable oil diet. Secondly, we found that linoleic and alpha-linolenic acids added to HG/FF reduced the specific activity of D6D. In contrast, when pure UFAs were added to an HS base, D6D specific activities remained unchanged or increased. Concordant results were obtained on D6D mRNA expression. Altogether, this study evidenced the importance of the nutritional status in D6D regulation by C18 UFAs: when used as control, HG/FF diet stimulates D6D compared with a standard control diet containing starch and 4% fats, leading to an overestimation of the D6D regulation by UFAs. Then, UFAs should be considered as repressors for unsaturated fatty acid biosynthesis only in very specific nutritional conditions.
authors.affiliation: INSERM UMR 866/Université de Bourgogne, Lipides Nutrition Cancer, 6 Bd Gabriel, 21000 Dijon, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Skrzypski
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bellenger
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bellenger
authors.forename: Andrew
authors.initials: A
authors.lastname: Sinclair
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Poisson
authors.forename: Christian
authors.initials: C
authors.lastname: Tessier
authors.forename: Mickaël
authors.initials: M
authors.lastname: Rialland
authors.forename: Michel
authors.initials: M
authors.lastname: Narce
mesh_terms: D000818:Animals; D004041:Dietary Fats; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology* / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D050602:Linoleoyl-CoA Desaturase