Probing single enzyme kinetics in real-time.
Studies of reaction kinetics at the single enzyme level have become possible due to the swift development of single molecule detection techniques. In this tutorial review, which targets both general and expert research chemists in the biochemical or physical (organic) chemistry field, we briefly introduce some of these fluorescence microscopic techniques and discuss selected studies on single molecule enzymology. The nature of the frequently observed fluctuations in the reaction rate, the so-called dynamic disorder, is described based on various available models.
Chen
Qi
Q
Department of Materials Science and Technology of Polymers, University of Twente, MESA+ Institute for Nanotechnology, PO Box 217, 7500 AE Enschede, The Netherlands.
Groote
Ramon
R
Schönherr
Holger
H
Vancso
G Julius
GJ
D004798:Enzymes; D007700:Kinetics; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological
D004798:Enzymes