Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos.
OBJECTIVE
To report a successful delivery after the transfer of a re-cryopreserved day-7 hatched blastocyst.


RESULTS
A 30-year-old woman underwent a long-treatment protocol for ovarian stimulation. Fourteen mature oocytes were obtained, and twelve were fertilized. On day 3, two cleaved embryos were transferred, but no implantation occurred. The remaining embryos were vitrified. Subsequently, two vitrified day-3 embryos were transferred. The woman became pregnant and delivered a healthy baby. Subsequently, two vitrified day-3 embryos were transferred, but no pregnancy occurred. Subsequently, all the remaining vitrified day-3 embryos were cultured. On day 5, no blastocyst was obtained. The remaining embryos were continued to be cultured. On day 7, a hatched blastocyst was obtained and re-vitrified. Subsequently, the re-vitrified day-7 hatched blastocyst was transferred. The woman became pregnant and delivered a healthy female.


CONCLUSIONS
The day-7 hatched blastocyst developed from vitrified embryos can be re-vitrified and have pregnancy potential after re-warming.
Hiraoka
Kenichiro
K
Kinutani Women's Clinic, Naka-ku, Hiroshima 730-0035, Japan. hiraoka@chive.ocn.ne.jp
Hiraoka
Kaori
K
Horiuchi
Toshitaka
T
Kusuda
Tomoyo
T
Okano
Shinichiro
S
Kinutani
Masayuki
M
Kinutani
Kazuo
K
D000328:Adult; D001755:Blastocyst / Q000166:cytology*; D015925:Cryopreservation / Q000379:methods*; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome
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