Oxidative stress in brain according to traumatic brain injury intensity.
BACKGROUND
The mechanisms of brain damage and neuroplasticity following traumatic brain injury (TBI) are complex and not completely understood. Thus, we investigated markers of oxidative stress in the central nervous system after mild and severe TBI in rats.


METHODS
Adult male wistar rats (five animals per group) submitted to mild (mTBI group) or severe TBI (sTBI Group) were sacrificed 30 min, 3, 6, or 12 h after the injury to quantify markers of oxidative damage in different brain regions. Levels of thiobarbituric acid reactive species and protein carbonyl in the cortex, hippocampus, striatum, and cerebellum of mTBI and sTBI groups were compared with the control group.


RESULTS
After mTBI, levels of protein oxidation were increased in all analyzed structures in several different times after injury. The increase in TBARS levels was not so consistent in mTBI. In contrast, sTBI did not induce a sustainable increase in oxidative damage markers in all analyzed structures.


CONCLUSIONS
Oxidative damage seemed to be inversely proportional to severity of traumatic brain injury.
Petronilho
Fabricia
F
Laboratorio de Fisiopatologia Experimental, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
Feier
Gustavo
G
de Souza
Bruna
B
Guglielmi
Cecília
C
Constantino
Larissa S
LS
Walz
Roger
R
Quevedo
João
J
Dal-Pizzol
Felipe
F
D000818:Animals; D015415:Biomarkers; D001930:Brain Injuries / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000503:physiopathology; D008297:Male; D018384:Oxidative Stress; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances