Functional analysis of Drosophila polytene chromosomes decompacted unit: the interband.
Differential compaction of the interphase chromosomes is important for proper functioning of the eukaryotic genome. Such non-uniform compaction is most easily observed in Drosophila salivary gland polytene chromosomes as a reproducible banding pattern. Functional mechanisms underlying the establishment and maintenance of the banding pattern remain unclear but have been hypothesized to involve transcription and chromatin insulators. We tested functional properties of DNA fragments from several transcriptionally inert interband regions that behave as autonomous decompacted units of polytene chromosomes. Our results suggest that, in the absence of transcription, the decondensed state of interband regions does not depend on the presence of insulator elements but instead correlates with the presence of transcriptional enhancers.
Berkaeva
Maria
M
Department of Molecular and Cell Biology, Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentyev ave, 8, 630090 Novosibirsk, Russia. berkaeva@bionet.nsc.ru
Demakov
Sergei
S
Schwartz
Yuri B
YB
Zhimulev
Igor
I
D000818:Animals; D030801:Animals, Genetically Modified; D002843:Chromatin / Q000235:genetics*; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D017344:Genes, Insect; D038101:Insulator Elements / Q000235:genetics; D014158:Transcription, Genetic
D002843:Chromatin; D004247:DNA
Cell. 1991 Mar 8;64(5):941-50
1848159
Annu Rev Genet. 2001;35:193-208
11700282
Genetika. 1998 Aug;34(8):1081-9
9777355
Mol Cell Biol. 2005 Jul;25(13):5590-8
15964814
EMBO J. 2003 Jun 16;22(12):3113-21
12805225
Mol Cell Biol. 1998 Dec;18(12):7478-86
9819433
Mol Cell. 2008 Oct 10;32(1):1-9
18851828
Mol Gen Genet. 1981;181(3):319-24
6787391
Chromosoma. 1980;80(3):253-75
6777121
Chromosoma. 2001 Jul;110(3):186-96
11513293
Cell. 1984 Nov;39(1):149-56
6207936
J Biol Chem. 1996 Oct 25;271(43):26971-80
8900183
Genes Dev. 1993 Oct;7(10):1966-78
7916729
Science. 2001 Jan 19;291(5503):495-8
11161206
Mol Cell. 2007 Dec 14;28(5):761-72
18082602
Cell. 2003 Apr 4;113(1):25-36
12679032
J Biol Chem. 1993 Jan 25;268(3):2092-9
8093616
Int Rev Cytol. 2004;241:203-75
15548421
PLoS Biol. 2008 Dec 23;6(12):2896-910
19108610
Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):683-7
1731341
J Biol Chem. 1995 Jun 30;270(26):15808-14
7797583
Genetica. 2004 Nov;122(3):311-24
15609554
Mol Cell. 2005 Oct 7;20(1):105-16
16209949
Science. 1982 Oct 22;218(4570):348-53
6289436
Cell. 2008 Jul 11;134(1):74-84
18614012
Chromosoma. 1999 Nov;108(6):375-83
10591997
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5223-8
12700350
Exp Mol Med. 2002 Sep 30;34(4):259-64
12515390
Development. 2000 Feb;127(4):779-90
10648236
Cell. 1995 Jun 16;81(6):879-89
7781065
Mol Cell. 2007 Jul 20;27(2):332-8
17643381
Genetica. 2008 Mar;132(3):267-79
17657571
Genetics. 2000 Jul;155(3):1297-311
10880489