title: In vivo monitoring of mRNA movement in Drosophila body wall muscle cells reveals the presence of myofiber domains.
abstract: BACKGROUND
  In skeletal muscle each muscle cell, commonly called myofiber, is actually a large syncytium containing numerous nuclei. Experiments in fixed myofibers show that mRNAs remain localized around the nuclei in which they are produced.
  
  
  RESULTS
  In this study we generated transgenic flies that allowed us to investigate the movement of mRNAs in body wall myofibers of living Drosophila embryos. We determined the dynamic properties of GFP-tagged mRNAs using in vivo confocal imaging and photobleaching techniques and found that the GFP-tagged mRNAs are not free to move throughout myofibers. The restricted movement indicated that body wall myofibers consist of three domains. The exchange of mRNAs between the domains is relatively slow, but the GFP-tagged mRNAs move rapidly within these domains. One domain is located at the centre of the cell and is surrounded by nuclei while the other two domains are located at either end of the fiber. To move between these domains mRNAs have to travel past centrally located nuclei.
  
  
  CONCLUSIONS
  These data suggest that the domains made visible in our experiments result from prolonged interactions with as yet undefined structures close to the nuclei that prevent GFP-tagged mRNAs from rapidly moving between the domains. This could be of significant importance for the treatment of myopathies using regenerative cell-based therapies.
authors.affiliation: Laboratory of Gene Expression and Imaging, Department of Molecular Cell Biology, Leiden University Medical C, Leiden, The Netherlands.
authors.forename: Alice M C
authors.initials: AM
authors.lastname: van Gemert
authors.forename: Annelies M A
authors.initials: AM
authors.lastname: van der Laan
authors.forename: Gonneke S K
authors.initials: GS
authors.lastname: Pilgram
authors.forename: Lee G
authors.initials: LG
authors.lastname: Fradkin
authors.forename: Jasprina N
authors.initials: JN
authors.lastname: Noordermeer
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Tanke
authors.forename: Carolina R
authors.initials: CR
authors.lastname: Jost
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017931:DNA Primers; D004330:Drosophila / Q000196:embryology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009132:Muscles / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins
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