title: A new 2S albumin from Jatropha curcas L. seeds and assessment of its allergenic properties.
abstract: Significant effort has been made world-wide to boost biofuels with the expectation of a positive contribution to renewable fuel and greenhouse gas reduction. Jatropha curcas L. has proved to be an opportunistic crop in tropical areas, particularly in unfavorable environments. For this reason, analyses of toxicity and allergy caused by its seeds and pollen are necessary. A 12kDa, allergenic 2S albumin, denoted Jat c 1, was isolated from Physic nut (J. curcas) seeds. Jat c 1 binds IgE attached to rat mast cells, inducing histamine release. It also showed strong cross-reactivity with the major allergens from castor bean, Ric c 1 and Ric c 3.
authors.affiliation: Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense - Darcy Ribeiro, 2000 Parque Califórnia, RJ CEP 28013-602, Brazil.
authors.forename: Fábio Menezes
authors.initials: FM
authors.lastname: Maciel
authors.forename: Mariana Agra
authors.initials: MA
authors.lastname: Laberty
authors.forename: Natália Deus
authors.initials: ND
authors.lastname: Oliveira
authors.forename: Shayany Pinto
authors.initials: SP
authors.lastname: Felix
authors.forename: Alexandra Martins Dos Santos
authors.initials: AM
authors.lastname: Soares
authors.forename: Maurício Afonso
authors.initials: MA
authors.lastname: Verícimo
authors.forename: Olga Lima Tavares
authors.initials: OL
authors.lastname: Machado
mesh_terms: D000418:Albumins / Q000276:immunology* / Q000302:isolation & purification; D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D002367:Ricinus communis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D031286:Jatropha / Q000737:chemistry / Q000276:immunology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011058:Pollen / Q000276:immunology / Q000378:metabolism; D051381:Rats; D012639:Seeds / Q000737:chemistry / Q000276:immunology
chemical_list: D000418:Albumins; D000485:Allergens; D010940:Plant Proteins