title: [Activated T cells promote differentiation of mesenchymal stem cells into osteoblasts].
abstract: This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1beta played an important role in the effect of activated T cells on MSCs, while TNF-alpha, TGF-beta1 were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interactive influence between MSCs and immune cells can be mediated through cytokines.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Jiang
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Su
authors.forename: Si-Wei
authors.initials: SW
authors.lastname: Huo
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiu-Sen
authors.initials: XS
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Mao
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D053583:Interleukin-1beta