title: Collagen/silk fibroin bi-template induced biomimetic bone-like substitutes.
abstract: A novel bi-template induced co-assembly method was employed to fabricate biomimetic bone substitute materials, collagen (COL)-fibroin/hydroxyapatite (COL-SF/HA) composite by using a combination of Type I COL and silk fibroin (SF) molecular templates. As a control, COL/HA and SF/HA composites were also synthesized via single-template assembly technology. The structure and morphology of the resulting assembly composites were investigated by X-ray diffractometer, Fourier transform infrared spectra, transmission electron microscope, and thermogravimetric analysis. Their sizes and size distributions were measured by DLS. The results indicated that the mineral phases in COL-SF/HA, COL-HA, and SF-HA composites were needle-like nano-HA crystals. In comparison to those in COL-HA and SF-HA, the mineral phase in COL-SF/HA displayed smaller size and more narrow distribution. Of all above biomimetic composites, the HA was well assembled with molecular template(s), and the organic content of the composite was about 12%-20%, which was quite similar to the natural bone in composition. CD and SDS-PAGE were used to examine the secondary structure and subunit composition of template proteins. The results revealed that the spatial structure of co-assembly template proteins played a pivotal role in controlling and regulating HA crystal nucleation and growth. Based on the experimental results above, a possible co-assembly mechanism for the HA growing on fibrous bi-template proteins was suggested.
authors.affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Shenqi
authors.initials: S
authors.lastname: Wang
authors.forename: Shengmin
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D012831:Bombyx; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000737:chemistry; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D003460:Crystallization; D018360:Crystallography, X-Ray; D017886:Durapatite / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005349:Fibroins / Q000737:chemistry* / Q000648:ultrastructure; D008027:Light; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D018786:Bone Substitutes; D012996:Solutions; D003094:Collagen; D005349:Fibroins; D017886:Durapatite