title: In-depth characterization of prebiotic galacto-oligosaccharides by a combination of analytical techniques.
abstract: A commercial prebiotic galacto-oligosaccharide mixture (Vivinal GOS) was extensively characterized using a combination of analytical techniques. The different techniques were integrated to give complementary information on specific characteristics of the oligosaccharide mixture, ranging from global information on degree of polymerization (DP) to the identity and concentration of individual oligosaccharides. The coupling of high-performance anion-exchange chromatography (HPAEC) to mass spectrometry (MS) was determined to be especially suitable to assign the DP of individual oligosaccharides on the basis of their m/z values as well as their quantification using external standards. The combination of NMR spectroscopy and methylation analysis after isolation using size exclusion chromatography (SEC) and hydrophilic interaction liquid chromatography (HILIC) was used for identification. All DP2 compounds could be identified and quantified in this way as well as the main DP3 compounds.
authors.affiliation: TNO Quality of Life, Utrechtseweg 48, 3704 HE, Zeist, The Netherlands. leon.coulier@tno.nl
authors.forename: Leon
authors.initials: L
authors.lastname: Coulier
authors.forename: Johan
authors.initials: J
authors.lastname: Timmermans
authors.forename: Richard
authors.initials: R
authors.lastname: Bas
authors.forename: Ron
authors.initials: R
authors.lastname: Van Den Dool
authors.forename: Ingrid
authors.initials: I
authors.lastname: Haaksman
authors.forename: Bert
authors.initials: B
authors.lastname: Klarenbeek
authors.forename: Ted
authors.initials: T
authors.lastname: Slaghek
authors.forename: William
authors.initials: W
authors.lastname: Van Dongen
mesh_terms: D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005690:Galactose / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D056692:Prebiotics / Q000032:analysis*
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics; D005690:Galactose