Characterization of mitochondrial control region, two intergenic spacers and tRNAs of Zaprionus indianus (Diptera: Drosophilidae).
The control region in insects is the major noncoding region in animal mitochondrial DNA (mtDNA), and is responsible for a large part of the variation in the DNA sequence and size of the genome of this organelle. In this study, the mtDNA control region, two intergenic spacers and tRNA genes of a Zaprionus indianus strain were cloned, sequenced and compared with other Drosophila species. The overall A+T content in the Z. indianus control region is 94.3%, and a comparison with other Drosophila species demonstrated that the most conserved region appears to be the 420 base pairs nearest to the tRNA(ile), similar to the findings of other authors. We also describe conserved sequence blocks, including a poly-T involved in the replication process of Drosophila mtDNA; a putative secondary structure also involved in the replication process and repeated sequences. tRNA(ile) sequence demonstrated the greatest variability when the tRNA sequences of species were compared.
da Silva
Norma Machado
NM
Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, P.O. Box 6010, Campinas, SP, 13083-875, Brazil. normams@unicamp.br
de Souza Dias
Aline
A
da Silva Valente
Vera Lúcia
VL
Valiati
Victor Hugo
VH
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D017124:Conserved Sequence; D021901:DNA, Intergenic / Q000235:genetics*; D018428:Drosophilidae / Q000235:genetics*; D005260:Female; D019901:Locus Control Region / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D021901:DNA, Intergenic; D012343:RNA, Transfer
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