Identification of flagellar motility genes in Yersinia ruckeri by transposon mutagenesis.
Here we demonstrate that flagellar secretion is required for production of secreted lipase activity in the fish pathogen Yersinia ruckeri and that neither of these activities is necessary for virulence in rainbow trout. Our results suggest a possible mechanism for the emergence of nonmotile biotype 2 Y. ruckeri through the mutational loss of flagellar secretion.
Evenhuis
Jason P
JP
National Center for Cool and Cold Water Aquaculture, Agricultural Research Service, U.S. Department of Agriculture, Kearneysville, West Virginia 25430, USA.
Lapatra
Scott E
SE
Verner-Jeffreys
David W
DW
Dalsgaard
Inger
I
Welch
Timothy J
TJ
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology; D005407:Flagella / Q000235:genetics* / Q000502:physiology*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009068:Movement; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D017686:Oncorhynchus mykiss / Q000382:microbiology; D016366:Open Reading Frames; D014774:Virulence / Q000235:genetics / Q000502:physiology; D044124:Yersinia ruckeri / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*
D017931:DNA Primers; D004269:DNA, Bacterial; D008049:Lipase
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