Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation.
To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding beta-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.
Luo
Lian Hua
LH
Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580-185, Jeonbuk, South Korea.
Seo
Pil-Soo
PS
Seo
Jeong-Woo
JW
Heo
Sun-Yeon
SY
Kim
Dae-Hyuk
DH
Kim
Chul Ho
CH
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D054889:Fatty Acid Synthase, Type II; D005227:Fatty Acids / Q000378:metabolism*; D018628:Gene Dosage; D005818:Genetic Engineering; D006836:Hydro-Lyases / Q000235:genetics* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D000431:Ethanol; D006836:Hydro-Lyases; C047118:3-hydroxyacyl-(acyl-carrier-protein) dehydratase; D054889:Fatty Acid Synthase, Type II