Arabidopsis ACBP6 is an acyl-CoA-binding protein associated with phospholipid metabolism.
In our recent paper in Plant Physiology, we showed that the Arabidopsis thaliana 10-kD acyl-CoA-binding protein, ACBP6, is subcellularly localized to the cytosol and that the overexpression of ACBP6 in transgenic Arabidopsis enhanced freezing tolerance. ACBP6-conferred freezing tolerance was independent of induced cold-regulated (COLD-RESPONSIVE) gene expression, but was correlated to an enhanced expression of phospholipase Ddelta (PLDdelta). Lipid analyses on cold-acclimated freezing-treated ACBP6-overexpressors revealed a decline in phosphatidylcholine (PC) and an elevation of phosphatidic acid (PA) in comparison to wild type. Furthermore, the His-tagged ACBP6 recombinant protein was observed using in vitro filter-binding assays to bind PC, but not PA or lysophosphatidylcholine. Taken together, our results implicate roles for ACBP6 in phospholipid metabolism that is related to gene regulation and PC-binding/transfer. This represents the first report demonstrating the in vitro binding of an ACBP to a phospholipid. The effect of ACBP6 on PLDdelta expression is reminiscent of yeast 10-kD ACBP function in the regulation of genes associated with stress responses, fatty acid synthesis and phospholipid synthesis. However, the yeast ACBP regulates the expression of genes involved in phospholipid synthesis by donation of acyl-CoA esters and its binding to phospholipids remains to be demonstrated.
Chen
Qin-Fang
QF
School of Biological Sciences; The University of Hong Kong; Hong Kong China.
Xiao
Shi
S
Chye
Mee-Len
ML
Biochim Biophys Acta. 1987 Sep 18;903(1):68-77
3651458
Eur J Biochem. 1991 Aug 1;199(3):489-509
1868841
Arch Biochem Biophys. 1996 Jul 1;331(1):55-62
8660683
J Biol Chem. 2002 Aug 30;277(35):31994-2002
12077151
Biochemistry. 1999 Oct 26;38(43):14131-7
10571986
Phytochemistry. 2004 Jun;65(12):1795-804
15276438
Plant J. 1999 Apr;18(2):205-14
10363372
Plant Mol Biol. 2004 May;55(2):297-309
15604682
Mol Biol Cell. 2006 Oct;17(10):4318-29
16870706
Curr Opin Cell Biol. 2008 Aug;20(4):360-70
18490149
Plant Mol Biol. 1998 Nov;38(5):827-38
9862500
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3531-5
6304714
J Lipid Res. 1981 Mar;22(3):391-403
7017050
Planta. 2006 Apr;223(5):871-81
16231156
Biochem J. 1997 Apr 1;323 ( Pt 1):1-12
9173866
FEBS Lett. 2006 Oct 9;580(23):5436-41
16828756
Biochem J. 2007 Oct 15;407(2):219-30
17593018
Plant J. 2008 Apr;54(1):141-51
18182029
Microbiology (Reading). 2006 Aug;152(Pt 8):2355-2363
16849800
Plant Mol Biol. 2004 Jan;54(2):233-43
15159625
Plant Mol Biol. 2003 Mar;51(4):483-92
12650615
J Exp Bot. 2006;57(11):2571-6
16804052
Nat Biotechnol. 2004 Apr;22(4):427-33
15004566
Plant Mol Biol. 2000 Dec;44(6):711-21
11202434