[Determination of content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes by RP-HPLC method].
OBJECTIVE
To establish a RP-HPLC method for content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes.


METHODS
The separation was performed with a COSMOSIL 5 C18-MS-II column (4.6 mm x 250 mm, 5 mmicrom) using methanol-water (95:5) as the mobile phase and detected at 203 nm. The flow rate was 1.0 mL x min(-1) and 50 microL sample solution was injected for each time.


RESULTS
The calibration curve was linear within the range 0.1-0.5 mg x mL(-1) (r = 0. 9999) , the intra-day RSD and inter-day RSD were less than 2% and the average recovery was between 101.44%-103.11% (n = 3).


CONCLUSIONS
The method is simple, accurate, sensitive and applicable for determination of content and entrapment efficiency of 20 (S)-protopanaxadiol pharmacosomes.
Han
Meihua
M
Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100193, China. hanmeihua727@163.com
Chen
Jing
J
Chen
Shilin
S
Wang
Xiangtao
X
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005894:Panax / Q000737:chemistry*; D015203:Reproducibility of Results; D012502:Sapogenins / Q000032:analysis* / Q000302:isolation & purification*
D004365:Drugs, Chinese Herbal; D012502:Sapogenins; C062916:protopanaxadiol