title: Genomic analyses of recombinant adenovirus type 11a in China.
abstract: Whole-genome sequencing of human adenovirus type 11 (HAdV-11) strain QS, isolated in China, was conducted, and its sequence was compared with the sequences of strains within the species of HAdVs. The HAdV-11 QS genome contains 34,755 nucleotides. Similar to the other HAdV subgenus B sequences, the HAdV-11 QS genome coded 37 functional proteins and could be divided into four early, two intermediate, and five late transcription regions. The amino acid sequences of the fiber and the hypervariable regions (HVRs) within the hexon gene of HAdV-11 QS were identical to the corresponding sequences of the HAdV-11a strain; further analyses that compared those amino acid sequences with the amino acid sequences of the HAdV species subgenus B:2 strains revealed that the highest degree of homology (>99.2%) existed between HAdV-11 QS and the prototypical HAdV-14 strain, except for a few coding sequences of HVRs within the hexon gene, DNA polymerase, pVI, and pre-terminal protein. This indicate that HAdV-11 strain QS, isolated in China, is a recombinant adenovirus of HAdV-14, and the recombination analyses also confirmed this finding. It is difficult to clarify the time and manner of the recombination, and further investigations are required to determine whether the emergence of recombination between HAdV-11a and HAdV-14 might increase virulence, thereby posing a new global challenge with regard to acute respiratory diseases in the near future.
authors.affiliation: State Key Laboratory for Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 27, Nanwei Road, Xuanwu District, Beijing 100050, People's Republic of China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Liuying
authors.initials: L
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Tan
authors.forename: Pengbo
authors.initials: P
authors.lastname: Yu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Tian
authors.forename: Jingjun
authors.initials: J
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dexin
authors.initials: D
authors.lastname: Li
authors.forename: Wenbo
authors.initials: W
authors.lastname: Xu
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins
references.citation: J Virol. 2005 Feb;79(4):2559-72
references.pmid: 15681456
references.citation: Mol Cell. 2005 Jan 7;17(1):121-35
references.pmid: 15629723
references.citation: J Med Virol. 2005 Oct;77(2):232-7
references.pmid: 16121380
references.citation: Mol Ther. 2000 Jan;1(1):18-30
references.pmid: 10933908
references.citation: Hum Gene Ther. 2004 Dec;15(12):1167-76
references.pmid: 15684694
references.citation: J Virol. 2002 Nov;76(21):10608-16
references.pmid: 12368303
references.citation: J Virol. 2002 Oct;76(19):10056-9
references.pmid: 12208985
references.citation: J Clin Microbiol. 2009 Mar;47(3):697-703
references.pmid: 19109466
references.citation: J Gen Virol. 2004 Sep;85(Pt 9):2615-2625
references.pmid: 15302955
references.citation: J Gen Virol. 2008 Feb;89(Pt 2):380-388
references.pmid: 18198368
references.citation: Clin Infect Dis. 2008 Feb 1;46(3):421-5
references.pmid: 18173356
references.citation: J Virol. 2003 Sep;77(17):9183-91
references.pmid: 12915534
references.citation: Cell. 1993 Apr 23;73(2):309-19
references.pmid: 8477447
references.citation: Virology. 2003 Apr 25;309(1):152-65
references.pmid: 12726735
references.citation: J Virol. 1976 Aug;19(2):643-58
references.pmid: 957484
references.citation: Intervirology. 1991;32(6):338-50
references.pmid: 1657824
references.citation: J Biol Chem. 1991 Aug 15;266(23):15075-9
references.pmid: 1869542
references.citation: J Virol. 2001 Nov;75(21):10446-54
references.pmid: 11581412
references.citation: J Virol. 1998 Oct;72(10):7860-70
references.pmid: 9733823
references.citation: J Virol. 2007 Jun;81(11):5978-84
references.pmid: 17360747
references.citation: J Virol. 1996 Mar;70(3):1396-405
references.pmid: 8627656
references.citation: J Virol. 2001 Aug;75(15):7131-41
references.pmid: 11435594
references.citation: J Trop Pediatr. 2004 Oct;50(5):279-84
references.pmid: 15510759
references.citation: Virology. 1995 Feb 20;207(1):32-45
references.pmid: 7871747
references.citation: J Virol. 1990 Jun;64(6):2732-42
references.pmid: 2335816
references.citation: EMBO J. 1992 Feb;11(2):761-8
references.pmid: 1537347
references.citation: Epidemiol Infect. 1988 Dec;101(3):503-9
references.pmid: 2850936
references.citation: J Gen Virol. 2000 Nov;81(Pt 11):2573-2604
references.pmid: 11038369
references.citation: J Virol. 1989 Sep;63(9):3612-21
references.pmid: 2760976
references.citation: J Mol Biol. 1989 Jul 5;208(1):195-8
references.pmid: 2769752
references.citation: Biochemistry. 2002 Jan 15;41(2):652-9
references.pmid: 11781106
references.citation: EMBO J. 1983;2(8):1357-65
references.pmid: 10872331
references.citation: J Infect Dis. 2007 Nov 15;196(10):1465-73
references.pmid: 18008225
references.citation: J Med Virol. 1996 Feb;48(2):151-6
references.pmid: 8835348