Proteomic analysis of date palm (Phoenix dactylifera L.) responses to endophytic colonization by entomopathogenic fungi.
The entomopathogenic fungi Beauveria bassiana, Lecanicillium dimorphum and L. cf. psalliotae can survive and colonize living palm tissue as endophytes. The molecular interaction between these biocontrol agent fungi and the date palm Phoenix dactylifera L. was investigated using proteomic techniques. Field date palms inoculated with these fungi were analyzed 15 and 30 days after inoculation in two independent bioassays. In vitro date palms were also inoculated with B. bassiana or L. cf. psalliotae. Qualitative and quantitative differences in protein accumulation between controls (not inoculated) and inoculated palms were found using 2-DE analysis, and some of these responsive proteins could be identified using MALDI/TOF-TOF. Proteins involved in plant defence or stress response were induced in P. dactylifera leaves as a response to endophytic colonization by entomopathogenic fungi in field date palms. Proteins related with photosynthesis and energy metabolism were also affected by entomopathogenic fungi colonization. A myosin heavy chain-like protein was accumulated in in vitro palms inoculated with these fungi. This suggests that endophytic colonization by these entomopathogenic fungi modulates plant defence responses and energy metabolism in field date palms and possibly modulates the expression of cell division-related proteins in in vitro palms at proteomic level.
Gómez-Vidal
Sonia
S
Multidisciplinary Institute for Environmental Studies "Ramón Margalef," Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, Spain.
Salinas
Jesús
J
Multidisciplinary Institute for Environmental Studies "Ramón Margalef," Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, Spain.
Tena
Manuel
M
Department of Biochemistry and Molecular Biology, ETSIAM, University of Córdoba, Córdoba, Spain.
Lopez-Llorca
Luis Vicente
LV
Multidisciplinary Institute for Environmental Studies "Ramón Margalef," Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, Spain.
D028023:Arecaceae / Q000378:metabolism* / Q000382:microbiology*; D052982:Beauveria / Q000254:growth & development*; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D006999:Hypocreales / Q000254:growth & development*; D010788:Photosynthesis; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis / Q000502:physiology*
D010940:Plant Proteins
Howard, F. W., Moore, D., Giblin-Davis, R. M., Abad, R. G., Insects on Palms, CAB International, Oxon, UK 2001.
Armengol, J., Abad, P., García-Jiménez, J., Boletin SEF 2005, 51, 3-5.
Armengol, J., Moretti, A., Perrone, G., Vicent, A., Bengoechea, J. A., García-Jiménez, J., 2005b. Eur. J. Plant. Pathol. 2005, 112, 123-131.
Abdullah, S. K., Asensio, L., Monfort, E., Gómez-Vidal, S., Palma-Guerrero, J., Salinas, J., Lopez-Llorca, L. V. et al., J. Phytopathol. 2005, 153, 417-422.
Asensio, L., Lopez-Llorca, L. V., López-Jiménez, J. A., Micron 2005, 36, 169-175.
Gómez-Vidal, S., Lopez-Llorca, L. V., Jansson, H.-B., Salinas, J., Micron 2006, 37, 624-632.
Wagner, B. L., Lewis, L. C., Microbiology 2000, 66, 3468-3473.
Quesada-Moraga, E., Landa, B. B., Muñoz-Ledesma, J., Jiménez-Diáz, R. M., Santiago-Álvarez, C., Mycopathologia 2006, 161, 323-329.
Akello, J., Dubois, T., Gold, C. S., Coyne, D., Akavuma J., Paparu, P., J. Invertebr. Pathol. 2007, 96, 34-42.
Posada, F., Aime, M. C., Peterson, F. W., Rehner, S. A., Vega, F. E., Mycol. Res. 2007, 3, 748-757.
Cherry, A. J., Banito, A., Djegui, D., Lomer, C., Int. J. Pest Manage. 2004, 50, 67-73.
Lopez-Llorca, L. V., Jansson, H.-B., Maciá-Vicente, J. G., Salinas, J., in: Schulz, B., Boyle, C., Sieber, T. (Eds.), Nematophagous Fungi as Root Endophytes in Microbial Root Endophytes, Springer, Berlin, Germany 2006, pp. 191-206.
Cánovas, F. M., Dumas-Gaudot, E., Recorbet, G., Jorrin, J., Mock, H.-P., Rossignol, M., Proteomics 2004, 4, 285-298.
Rossignol, M., Peltier, J. B., Mock, H. P., Matros, A., Maldonado, A. M., Jorrín, J. V., Proteomics 2006, 6, 5529-5548.
Jorrín, J. V., Maldonado, A. M., Castillejo, M. A., Proteomics 2007, 7, 2947-2962.
Chinnasamy, G., in: Siddiqui Z. A. (Ed.), A Proteomics Perspective on Biocontrol and Plant Defense Mechanism in PGPR: Biocontrol and Biofertilization, Springer Science, Dordrecht, The Netherlands 2005, pp. 233-255.
Marra, R., Ambrosino, P., Carbone, V., Vinale, F., Woo, S. L., Ruocco, M., Ciliento, R. et al., Curr. Genet. 2006, 50, 307-321.
Segarra, G., Casanova, E., Bellido, D., Odena, M. A., Oliveira, E., Trillas, I., Proteomics 2007, 7, 3943-3952.
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., Lorito, M., Soil Biol. Biochem. 2008, 40, 1-10.
Gómez-Vidal, S., Tena, M., Lopez-Llorca, L. V., Salinas, J., Electrophoresis 2008, 29, 448-456.
Laemmli, U. K., Nature 1970, 227, 680-685.
Heukeshoven, J., Dernick, R., Electrophoresis 1985, 6, 103-112.
Ding, J., Cheng, H., Araki, H., Yang, Y., Tian D., Genetica 2007, 129, 235-242.
Martin, G. B., Bogdanove, A. J., Sessa, G., Annu. Rev. Plant Biol. 2003, 54, 23-61.
Jones, J. D. G., Dangl, J. L., Nature 2006, 444, 323-329.
Eveno, E., Collada, C., Guevara, M. A., Léger, V., Soto, A., Díaz, L., Léger, P. et al., Mol. Biol. Evol. 2008, 25, 417-437.
Bermúdez-Moretti, M., Maskin, L., Gudesblat, G., Correa-García, S., Lusem, N. D., Physiol. Plant 2006, 127, 111-118.
Collada, C., Gomez, L., Casado, R., Aragoncillo, C., Plant Physiol. 1997, 115, 71-77.
Wang, D., Eyles, A., Mandich, D., Bonello, P., Physiol. Mol. Plant Pathol. 2006, 68, 149-157.
Roose, J. L., Wegener, K. M., Pakrasi, H. B., Photosynth. Res. 2007, 92, 369-387.
Portis, A. R., Jr., Photosynth. Res. 2003, 75, 11-27.
Torres, M. A., Jones, J. D. G., Dangl, J. L., Plant Physiol. 2006, 141, 373-378.
Ojangu, E.-L., Jarve, K., Paves, H., Truve, E., Protoplasma 2007, 230, 193-202.
Ernst, M., Mendgen, K. W., Wirsel, S. G. R., Mol. Plant Microbe Interact. 2003, 16, 580-587.
Conrath, U., Beckers, G. J. M., Flors, V., García-Agustín, P., Jakab, G., Mauch, F., Newman, M.-A. et al., Mol. Plant Microbe Interact. 2006, 19, 1062-1071.