title: Chances and limitations of wild bird monitoring for the avian influenza virus H5N1--detection of pathogens highly mobile in time and space.
abstract: Highly pathogenic influenza virus (HPAIV) H5N1 proved to be remarkably mobile in migratory bird populations where it has led to extensive outbreaks for which the true number of affected birds usually cannot be determined. For the evaluation of avian influenza monitoring and HPAIV early warning systems, we propose a time-series analysis that includes the estimation of confidence intervals for (i) the prevalence in outbreak situations or (ii) in the apparent absence of disease in time intervals for specified regional units. For the German outbreak regions in 2006 and 2007, the upper 95% confidence limit allowed the detection of prevalences below 1% only for certain time intervals. Although more than 25,000 birds were sampled in Germany per year, the upper 95% confidence limit did not fall below 5% in the outbreak regions for most of the time. The proposed analysis can be used to monitor water bodies and high risk areas, also as part of an early-warning system. Chances for an improved targeting of the monitoring system as part of a risk-based approach are discussed with the perspective of reducing sample sizes.
authors.affiliation: Institute of Epidemiology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Wusterhausen, Germany.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Wilking
authors.forename: Mario
authors.initials: M
authors.lastname: Ziller
authors.forename: Christoph
authors.initials: C
authors.lastname: Staubach
authors.forename: Anja
authors.initials: A
authors.lastname: Globig
authors.forename: Timm C
authors.initials: TC
authors.lastname: Harder
authors.forename: Franz J
authors.initials: FJ
authors.lastname: Conraths
mesh_terms: D000818:Animals; D000835:Animals, Wild; D001717:Birds / Q000821:virology*; D004784:Environmental Monitoring / Q000379:methods*; D005858:Germany; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification*
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