title: SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages.
abstract: This work was designed to study the role of surfactant protein D in the regulation of NO synthesis by "non-alveolar" microphages. We evaluated whether the effects of surfactant protein D depend on the phenotype of macrophages. In the absence of surfactant protein D, the LPS-induced iNOS response was shown to decrease in macrophages of native and proinflammatory phenotypes by 30%, and in macrophages of the antiinflammatory phenotype (by 63%). Under the influence of lipopolysaccharide in high doses (500 ng/ml), NO(2)*- production by mouse macrophages without surfactant protein D was reduced in native cells (by 25%), but increased in proinflammatory (by 40%) and antiinflammatory phenotypes (by 12% compared to mouse macrophages with surfactant protein D). Our results suggest that surfactant protein D is involved in the immune response in the whole organism, but not only in the lungs. The effect of surfactant protein D depends on the phenotype of macrophages.
authors.affiliation: Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Atochina-Vasserman
authors.forename: E V
authors.initials: EV
authors.lastname: Abramova
authors.forename: Y
authors.initials: Y
authors.lastname: Tomer
authors.forename: P
authors.initials: P
authors.lastname: Scott
authors.forename: V A
authors.initials: VA
authors.lastname: Nazarov
authors.forename: S V
authors.initials: SV
authors.lastname: Kruglov
authors.forename: M F
authors.initials: MF
authors.lastname: Beers
authors.forename: A J
authors.initials: AJ
authors.lastname: Gow
authors.forename: I Yu
authors.initials: IY
authors.lastname: Malyshev
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology / Q000503:physiopathology*; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D009573:Nitrites; D037663:Pulmonary Surfactant-Associated Protein D; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse
references.citation: J Immunol. 2001 Jun 15;166(12):7514-9
references.pmid: 11390505
references.citation: J Immunol. 2001 Nov 15;167(10):5868-73
references.pmid: 11698462
references.citation: Biochim Biophys Acta. 1998 Nov 19;1408(2-3):278-89
references.pmid: 9813367
references.citation: Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11869-74
references.pmid: 9751757
references.citation: J Immunol. 2000 Feb 1;164(3):1442-50
references.pmid: 10640760
references.citation: J Leukoc Biol. 1993 Nov;54(5):444-50
references.pmid: 8228623
references.citation: Proc Natl Acad Sci U S A. 2000 May 23;97(11):5972-7
references.pmid: 10801980
references.citation: Am J Respir Cell Mol Biol. 2004 Mar;30(3):271-9
references.pmid: 12871850
references.citation: Annu Rev Physiol. 2001;63:521-54
references.pmid: 11181966
references.citation: J Immunol. 2000 Oct 1;165(7):3934-40
references.pmid: 11034401
references.citation: J Biol Chem. 2000 Dec 8;275(49):38848-55
references.pmid: 10969075
references.citation: J Infect Dis. 2004 Apr 15;189(8):1528-39
references.pmid: 15073692
references.citation: Am J Respir Cell Mol Biol. 1995 Apr;12(4):410-5
references.pmid: 7695920
references.citation: J Immunol. 2007 Dec 15;179(12):8090-7
references.pmid: 18056350
references.citation: Am J Respir Cell Mol Biol. 1994 Apr;10(4):430-6
references.pmid: 8136158