title: Synthesis and physicochemical characterization of a novel amphiphilic polylactic acid-hyperbranched polyglycerol conjugate for protein delivery.
abstract: Amphiphilic copolymers with polylactic acid (PLA) chains grafted onto hyperbranched polyglycerol (HPG) have been synthesized and characterized. The copolymer nanoparticles with corona and core structure were formed by self-assembly in aqueous solution. The loading capacity and association efficiency were up to 23% and 86%, respectively. Protein release profiles with different copolymer compositions and BSA concentrations all showed a burst effect followed by a continuous release phase. The released BSA from the copolymer nanoparticles remained in its original structure over a period of 4 days, as testified by circular dichroism spectroscopy. Furthermore, cell viability research suggested good biocompatibility of the copolymer nanoparticles, which have a promising potential for protein delivery system.
authors.affiliation: Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Gao
authors.forename: Xinge
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Wu
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Chaoxing
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002942:Circular Dichroism; D004337:Drug Carriers; D004339:Drug Compounding; D005990:Glycerol / Q000138:chemical synthesis* / Q000633:toxicity; D007700:Kinetics; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000633:toxicity; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D053758:Nanoparticles; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis* / Q000633:toxicity; D011487:Protein Conformation; D055550:Protein Stability; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility
chemical_list: D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; C043941:polyglycerol; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C033616:poly(lactide); D005990:Glycerol