title: A modified technique for heterotopic heart transplantation in rats.
abstract: BACKGROUND
  Heterotopic heart transplantation in rats has been widely used in various experiments since Ono and Lindsey published their technique [2]. Many modifications have been reported to improve this animal model. In this study, we described a new modified Ono and Lindsey technique.
  
  
  METHODS
  BN and Lewis rats weighing 200 to 250 g were used as donors and recipients. Donor heart was transplanted into the recipients' abdominal cavity, anastomosing the donor bracheocephalic trunk (BT) and the right pulmonary artery (RPA) to the recipient's abdominal artery (AA) and inferior vena cava (IVC). Graft function was evaluated by pulse palpation and histopathologic examination; intragraft cytokine mRNA expression was detected by quantitative PCR; the hemodynamics indexes of the recipients receiving our modified approach and traditional Ono and Lindsey technique were compared.
  
  
  RESULTS
  Thirty heart transplantations were carried out in our modified technique with three graft losses. The total operation can be finished within 1h. Both histopathologic findings and quantitative PCR results confirmed the presence of acute graft rejection. In addition, the hemodynamics indexes of recipients receiving our modified approach were nearer normal physiologic situation than those of recipients receiving traditional technique.
  
  
  CONCLUSIONS
  Our modified technique is an easily learned and reproduced procedure that allows less recipient surgical stress, a shorter operation time, and leads to a high graft and recipient survival rate.
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University Chengdu, Sichuan, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Shan
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.forename: Chengwen
authors.initials: C
authors.lastname: Li
authors.forename: Nengwen
authors.initials: N
authors.lastname: Ke
authors.forename: Chuntao
authors.initials: C
authors.lastname: Zhang
authors.forename: Youping
authors.initials: Y
authors.lastname: Li
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D006084:Graft Rejection / Q000503:physiopathology; D006339:Heart Rate / Q000502:physiology; D016027:Heart Transplantation / Q000379:methods*; D008297:Male; D011183:Postoperative Complications / Q000503:physiopathology; D011651:Pulmonary Artery / Q000601:surgery*; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D020858:Tissue and Organ Harvesting / Q000379:methods*; D016042:Transplantation, Heterotopic; D014682:Vena Cava, Inferior / Q000601:surgery*