Decomposition of alpha-Tocopheryl Glycosides in Rat Tissues.
BACKGROUND: The aim of our investigation was to estimate the stability of alpha-tocopheryl O-glycosides in relation to activity of exoglycosidases in selected rat tissues. MATERIAL AND METHODS: Acetylated glycosides were obtained in glucosidation of alpha-tocopherol using the Helferich method. The activity of exoglycosidases was determined by the Zwierz et al. method. Protein concentrations were determined by the biuret method. The concentration of released alpha-tocopherol was determined with the HPLC method. RESULTS: The comparison of the amount of released alpha-tocopherol with the amount of released p-nitrophenol shows that glycoside bound in 2a-5a derivatives of alpha-tocopherol undergoes hydrolysis significantly harder than in appropriate 2b-5bp-nitrophenyl derivatives. CONCLUSION: The results indicate that tocopheryl O-glycosides are more resistant to enzymatic hydrolysis than appropriate p-nitrophenol O-glycosides 2a-5a. Among examined tocopheryl O-glycosides, galactoside 4 is the only compound that caused the significant increase in tocopherol concentration, as compared to its endogenic content.
Knaś
Małgorzata
M
Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Poland.
Wałejko
Piotr
P
Maj
Jadwiga
J
Hryniewicka
Agnieszka
A
Witkowski
Stanisław
S
Borzym-Kluczyk
Małgorzata
M
Dudzik
Danuta
D
Zwierz
Krzysztof
K
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