title: Comparative toxicity of mycotoxins to navel orangeworm (Amyelois transitella) and corn earworm (Helicoverpa zea).
abstract: Mycotoxins, such as aflatoxins and ochratoxins, are widely distributed in nature and are frequently problematic crop contaminants that cause millions of dollars of annual losses in the United States. Insect infestations of crop plants significantly exacerbate mycotoxin contamination. Damage to a variety of nut species by Amyelois transitella Walker (navel orangeworm, NOW) is associated with infection by Aspergillus species and concomitant production of aflatoxins and ochratoxins. Resistance to aflatoxins in this lepidopteran is compared here with the levels of resistance in Helicoverpa zea (corn earworm, CEW), another lepidopteran that routinely encounters aflatoxins in its diet, albeit at lower levels. Measured as the developmental delay caused by aflatoxin B1 (AFB1), it is apparent that the LC(50) (defined as the concentration preventing 50% of newly hatched larvae from entering the 2nd instar within 48 h) for AFB1 is 100 times greater for A. transitella than for H. zea. Similarly, A. transitella 1st instars display substantially higher tolerance to ochratoxin A, another mycotoxin contaminant produced by Aspergillus species, than do H. zea. Our studies indicate that A. transitella, although a hostplant generalist, may well be highly specialized for mycotoxin detoxification.
authors.affiliation: Department of Entomology, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Guodong
authors.initials: G
authors.lastname: Niu
authors.forename: Joel
authors.initials: J
authors.lastname: Siegel
authors.forename: Mary A
authors.initials: MA
authors.lastname: Schuler
authors.forename: May R
authors.initials: MR
authors.lastname: Berenbaum
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000383:Agriculture; D000818:Animals; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007915:Lepidoptera / Q000187:drug effects / Q000254:growth & development*; D009793:Ochratoxins / Q000633:toxicity*
chemical_list: D009793:Ochratoxins; D016604:Aflatoxin B1
references.citation: Carcinogenesis. 1984 Feb;5(2):269-76
references.pmid: 6421504
references.citation: Annu Rev Pharmacol Toxicol. 1994;34:135-72
references.pmid: 8042848
references.citation: J Agric Food Chem. 2001 Sep;49(9):4513-9
references.pmid: 11559163
references.citation: Biomed Chromatogr. 2008 Sep;22(9):1013-20
references.pmid: 18506683
references.citation: Pharmacol Ther. 1995 Feb;65(2):163-92
references.pmid: 7540767
references.citation: Mutat Res. 1998 Jun 18;402(1-2):121-8
references.pmid: 9675258
references.citation: Biochem Biophys Res Commun. 1992 Oct 30;188(2):597-603
references.pmid: 1445304
references.citation: J Insect Sci. 2004;4:40
references.pmid: 15861255
references.citation: Mycopathologia. 2006 Sep;162(3):215-23
references.pmid: 16944288
references.citation: Annu Rev Pharmacol Toxicol. 2005;45:51-88
references.pmid: 15822171
references.citation: J Invertebr Pathol. 1967 Mar;9(1):132-3
references.pmid: 6073675
references.citation: Toxicol Lett. 1995 Dec;82-83:861-7
references.pmid: 8597154
references.citation: Semin Liver Dis. 2004 Feb;24(1):77-88
references.pmid: 15085488
references.citation: Appl Microbiol. 1975 Jan;29(1):48-53
references.pmid: 1110492
references.citation: Chem Res Toxicol. 2005 Jul;18(7):1091-7
references.pmid: 16022501
references.citation: Arch Insect Biochem Physiol. 2008 Sep;69(1):32-45
references.pmid: 18615618
references.citation: J Invertebr Pathol. 1976 Mar;27(2):255-8
references.pmid: 815441
references.citation: Toxicol Appl Pharmacol. 1988 Jun 15;94(1):118-27
references.pmid: 3131927
references.citation: Adv Exp Med Biol. 2002;504:277-85
references.pmid: 11922095
references.citation: J Agric Food Chem. 2000 Feb;48(2):489-92
references.pmid: 10691662
references.citation: Arch Insect Biochem Physiol. 2000 Dec;45(4):166-74
references.pmid: 11223936
references.citation: J Invertebr Pathol. 1978 Jan;31(1):37-40
references.pmid: 415091
references.citation: J Chem Ecol. 2006 Jul;32(7):1459-71
references.pmid: 16830213
references.citation: Biochemistry. 1997 Mar 18;36(11):3056-60
references.pmid: 9115980
references.citation: Pest Manag Sci. 2003 Jun-Jul;59(6-7):629-42
references.pmid: 12846313
references.citation: Am J Clin Nutr. 2004 Nov;80(5):1106-22
references.pmid: 15531656