title: Mapping quantitative trait loci (QTLs) for fatty acid composition in an interspecific cross of oil palm.
abstract: BACKGROUND
  Marker Assisted Selection (MAS) is well suited to a perennial crop like oil palm, in which the economic products are not produced until several years after planting. The use of DNA markers for selection in such crops can greatly reduce the number of breeding cycles needed. With the use of DNA markers, informed decisions can be made at the nursery stage, regarding which individuals should be retained as breeding stock, which are satisfactory for agricultural production, and which should be culled. The trait associated with oil quality, measured in terms of its fatty acid composition, is an important agronomic trait that can eventually be tracked using molecular markers. This will speed up the production of new and improved oil palm planting materials.
  
  
  RESULTS
  A map was constructed using AFLP, RFLP and SSR markers for an interspecific cross involving a Colombian Elaeis oleifera (UP1026) and a Nigerian E. guinneensis (T128). A framework map was generated for the male parent, T128, using Joinmap ver. 4.0. In the paternal (E. guineensis) map, 252 markers (199 AFLP, 38 RFLP and 15 SSR) could be ordered in 21 linkage groups (1815 cM). Interval mapping and multiple-QTL model (MQM) mapping (also known as composite interval mapping, CIM) were used to detect quantitative trait loci (QTLs) controlling oil quality (measured in terms of iodine value and fatty acid composition). At a 5% genome-wide significance threshold level, QTLs associated with iodine value (IV), myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2) content were detected. One genomic region on Group 1 appears to be influencing IV, C14:0, C16:0, C18:0 and C18:1 content. Significant QTL for C14:0, C16:1, C18:0 and C18:1 content was detected around the same locus on Group 15, thus revealing another major locus influencing fatty acid composition in oil palm. Additional QTL for C18:0 was detected on Group 3. A minor QTL for C18:2 was detected on Group 2.
  
  
  CONCLUSIONS
  This study describes the first successful detection of QTLs for fatty acid composition in oil palm. These QTLs constitute useful tools for application in breeding programmes.
authors.affiliation: Advanced Biotechnology and Breeding Centre, Biology Division, Malaysian Palm Oil Board (MPOB), No, 6, Persiaran Institusi, Selangor DE, Malaysia. rajinder@mpob.gov.my
authors.forename: Rajinder
authors.initials: R
authors.lastname: Singh
authors.forename: Soon G
authors.initials: SG
authors.lastname: Tan
authors.forename: Jothi M
authors.initials: JM
authors.lastname: Panandam
authors.forename: Rahimah Abdul
authors.initials: RA
authors.lastname: Rahman
authors.forename: Leslie C L
authors.initials: LC
authors.lastname: Ooi
authors.forename: Eng-Ti L
authors.initials: ET
authors.lastname: Low
authors.forename: Mukesh
authors.initials: M
authors.lastname: Sharma
authors.forename: Johannes
authors.initials: J
authors.lastname: Jansen
authors.forename: Suan-Choo
authors.initials: SC
authors.lastname: Cheah
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D028023:Arecaceae / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D012150:Polymorphism, Restriction Fragment Length; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D005227:Fatty Acids; D005819:Genetic Markers
references.citation: BMC Plant Biol. 2008 May 29;8:62
references.pmid: 18507865
references.citation: Theor Appl Genet. 2002 Feb;104(2-3):338-349
references.pmid: 12582706
references.citation: Mol Genet Genomics. 2003 May;269(2):243-51
references.pmid: 12756536
references.citation: Nucleic Acids Res. 1996 Nov 1;24(21):4369-71
references.pmid: 8932400
references.citation: Theor Appl Genet. 2003 Jul;107(2):239-47
references.pmid: 12845439
references.citation: Genetics. 1990 Mar;124(3):743-56
references.pmid: 1968875
references.citation: Heredity (Edinb). 2003 Jan;90(1):39-48
references.pmid: 12522424
references.citation: Nat Genet. 1995 Nov;11(3):241-7
references.pmid: 7581446
references.citation: Theor Appl Genet. 2003 Nov;107(8):1433-41
references.pmid: 14513216
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Genetics. 1994 Aug;137(4):1121-37
references.pmid: 7982566
references.citation: Genome Res. 1998 Mar;8(3):175-85
references.pmid: 9521921
references.citation: Theor Appl Genet. 1995 Jun;90(7-8):933-47
references.pmid: 24173047
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: Theor Appl Genet. 2006 Aug;113(3):497-507
references.pmid: 16767448
references.citation: BMC Genomics. 2007 Oct 22;8:381
references.pmid: 17953740
references.citation: Genome. 2001 Jun;44(3):413-25
references.pmid: 11444700
references.citation: Theor Appl Genet. 2005 Feb;110(4):754-65
references.pmid: 15723275
references.citation: Genome. 1995 Oct;38(5):894-901
references.pmid: 18470215
references.citation: Genome. 1997 Feb;40(1):116-22
references.pmid: 18464812