Expression of a plant defensin in rice confers resistance to fungal phytopathogens.
Transgenic rice (Oryza sativa L. cv. Pusa basmati 1), overexpressing the Rs-AFP2 defensin gene from the Raphanus sativus was generated by Agrobacterium tumefaciens-mediated transformation. Expression levels of Rs-AFP2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. It was observed that constitutive expression of Rs-AFP2 suppresses the growth of Magnaporthe oryzae and Rhizoctonia solani by 77 and 45%, respectively. No effect on plant morphology was observed in the Rs-AFP2 expressing rice lines. The inhibitory activity of protein extracts prepared from leaves of Rs-AFP2 plants on the in vitro growth of M. oryzae indicated that the Rs-AFP2 protein produced by transgenic rice plants was biologically active. Transgene expression of Rs-AFP2 was not accompanied by an induction of pathogenesis-related (PR) gene expression, suggesting that the expression of Rs-AFP2 directly inhibits the pathogens. Here, we demonstrate that transgenic rice plants expressing the Rs-AFP2 gene show enhanced resistance to M. oryzae and R. solani, two of the most important pathogens of rice.
Jha
Sanjay
S
Centre for Genome Research, Department of Microbiology and Biotechnology Centre, Faculty of Science, The M S. University of Baroda, Vadodara, Gujarat, 390002, India.
Chattoo
Bharat B
BB
D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D023082:Defensins / Q000378:metabolism* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D007113:Immunity, Innate / Q000235:genetics*; D007150:Immunohistochemistry; D020082:Magnaporthe / Q000187:drug effects / Q000254:growth & development; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D031224:Raphanus / Q000235:genetics; D012232:Rhizoctonia / Q000187:drug effects / Q000254:growth & development
D023082:Defensins; D010936:Plant Extracts
Plant Cell. 1995 Jan;7(1):29-42
12242350
Plant Cell. 1995 May;7(5):573-88
7780308
J Biol Chem. 1992 Aug 5;267(22):15301-9
1639777
J Biol Chem. 2004 Feb 6;279(6):3900-5
14604982
Plant J. 1994 Aug;6(2):271-82
7920717
J Clin Invest. 2006 Jun;116(6):1651-9
16741577
Yeast. 1993 Jul;9(7):715-22
8368005
Planta. 2002 Dec;216(2):193-202
12447532
Plant J. 1996 Jul;10(1):165-74
8758986
Plant Physiol. 1995 Aug;108(4):1353-8
7659744
Curr Genet. 1992 Jan;21(1):83-4
1735128
Transgenic Res. 2009 Feb;18(1):59-69
18618285
FEBS Lett. 2002 Aug 14;525(1-3):59-64
12163162
Nature. 2004 Sep 30;431(7008):582-6
15457264
Plant Physiol. 2002 Apr;128(4):1346-58
11950983
Biochem Biophys Res Commun. 2002 Oct 18;298(1):46-53
12379218
Anal Biochem. 1976 May 7;72:248-54
942051
Transgenic Res. 1996 May;5(3):213-8
8673150
Theor Appl Genet. 2002 Nov;105(6-7):809-814
12582903
FEBS Lett. 1995 Jul 17;368(2):257-62
7628617
Plant J. 1995 Jul;8(1):97-109
7655510
Anal Biochem. 1987 May 15;163(1):16-20
2441623
J Mol Microbiol Biotechnol. 2007;13(4):243-7
17827975