title: Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells.
abstract: Responses of plant cells to environmental stresses often involve morphological changes, differentiation and redistribution of various organelles and cytoskeletal network. Tobacco BY-2 cells provide excellent model system for in vivo imaging of these intracellular events. Treatment of the cell cycle-synchronized BY-2 cells with a proteinaceous oomycete elicitor, cryptogein, induces highly synchronous programmed cell death (PCD) and provide a model system to characterize vacuolar and cytoskeletal dynamics during the PCD. Sequential observation revealed dynamic reorganization of the vacuole and actin microfilaments during the execution of the PCD. We further characterized the effects cryptogein on mitotic microtubule organization in cell cycle-synchronized cells. Cryptogein treatment at S phase inhibited formation of the preprophase band, a cortical microtubule band that predicts the cell division site. Cortical microtubules kept their random orientation till their disruption that gradually occurred during the execution of the PCD twelve hours after the cryptogein treatment. Possible molecular mechanisms and physiological roles of the dynamic behavior of the organelles and cytoskeletal network in the pathogenic signal-induced PCD are discussed.
authors.affiliation: Department of Integrated Biosciences; Graduate School of Frontier Sciences; The University of Tokyo; Kashiwanoha Kashiwa, Chiba Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Higaki
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kadota
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Goh
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Hayashi
authors.forename: Natsumaro
authors.initials: N
authors.lastname: Kutsuna
authors.forename: Toshio
authors.initials: T
authors.lastname: Sano
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Hasezawa
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Kuchitsu
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