title: The effect of anchoring sutures on medicinal leech mortality.
abstract: OBJECTIVE
  The implementation of leech therapy for surgical flaps is not always logistically easy or comfortable for patients or healthcare providers. We examine different methods of placing sutures in the medicinal leech, Hirudo medicinalis, to make the implementation of leech therapy easier.
  
  
  METHODS
  Sixteen leeches were randomly divided into 3 groups: a control group, a deep anchoring suture group, and a superficial anchoring suture group. The leeches were observed to determine if either of these methods had an adverse effect on survival compared with the control group.
  
  
  RESULTS
  No difference in survival time was observed across the different groups.
  
  
  CONCLUSIONS
  The placement of anchoring sutures in leeches can ease the implementation of leech therapy by allowing for greater control of the leeches and thus increased patient comfort.
authors.affiliation: New Jersey Medical School, University of Medicine & Dentistry of New Jersey, Newark, USA. davilavj@umdnj.edu
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Davila
authors.forename: Ian C
authors.initials: IC
authors.lastname: Hoppe
authors.forename: Rocco
authors.initials: R
authors.lastname: Landi
authors.forename: Frank S
authors.initials: FS
authors.lastname: Ciminello
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