title: Imprinting of the Y chromosome influences dosage compensation in roX1 roX2 Drosophila melanogaster.
abstract: Drosophila melanogaster males have a well-characterized regulatory system that increases X-linked gene expression. This essential process restores the balance between X-linked and autosomal gene products in males. A complex composed of the male-specific lethal (MSL) proteins and RNA is recruited to the body of transcribed X-linked genes where it modifies chromatin to increase expression. The RNA components of this complex, roX1 and roX2 (RNA on the X1, RNA on the X2), are functionally redundant. Males mutated for both roX genes have dramatically reduced survival. We show that reversal of sex chromosome inheritance suppresses lethality in roX1 roX2 males. Genetic tests indicate that the effect on male survival depends upon the presence and source of the Y chromosome, revealing a germ line imprint that influences dosage compensation. Conventional paternal transmission of the Y chromosome enhances roX1 roX2 lethality, while maternal transmission of the Y chromosome suppresses lethality. roX1 roX2 males with both maternal and paternal Y chromosomes have very low survival, indicating dominance of the paternal imprint. In an otherwise wild-type male, the Y chromosome does not appreciably affect dosage compensation. The influence of the Y chromosome, clearly apparent in roX1 roX2 mutants, thus requires a sensitized genetic background. We believe that the Y chromosome is likely to act through modulation of a process that is defective in roX1 roX2 mutants: X chromosome recognition or chromatin modification by the MSL complex.
authors.affiliation: Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA.
authors.forename: Debashish U
authors.initials: DU
authors.lastname: Menon
authors.forename: Victoria H
authors.initials: VH
authors.lastname: Meller
mesh_terms: D000818:Animals; D004303:Dosage Compensation, Genetic; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D007150:Immunohistochemistry; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008957:Models, Genetic; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C092595:Pabp2 protein, Drosophila; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C082132:msl-1 protein, Drosophila; C454144:roX1 protein, Drosophila
references.citation: Annu Rev Genet. 2005;39:615-51
references.pmid: 16285873
references.citation: Genes Dev. 2006 Apr 1;20(7):858-70
references.pmid: 16547172
references.citation: Annu Rev Genet. 1973;7:225-37
references.pmid: 4205904
references.citation: Science. 2006 Nov 24;314(5803):1292-5
references.pmid: 17124320
references.citation: Genes Dev. 2006 Apr 1;20(7):871-83
references.pmid: 16547175
references.citation: Dev Genes Evol. 2009 Feb;219(2):59-66
references.pmid: 19031081
references.citation: Genetics. 1998 Sep;150(1):283-99
references.pmid: 9725847
references.citation: Cell. 1995 Jun 16;81(6):867-77
references.pmid: 7781064
references.citation: Cell. 1999 Aug 20;98(4):513-22
references.pmid: 10481915
references.citation: Genetics. 2002 Nov;162(3):1245-58
references.pmid: 12454070
references.citation: PLoS Genet. 2008 May 02;4(5):e1000066
references.pmid: 18451980
references.citation: EMBO Rep. 2002 Mar;3(3):242-7
references.pmid: 11882543
references.citation: Genetics. 1999 Apr;151(4):1503-16
references.pmid: 10101173
references.citation: Mol Cell Biol. 2000 Jan;20(1):312-8
references.pmid: 10594033
references.citation: Science. 2008 Jan 4;319(5859):91-3
references.pmid: 18174442
references.citation: Mol Cell Biol. 2008 Aug;28(16):4952-62
references.pmid: 18541664
references.citation: Cell. 1997 Feb 21;88(4):445-57
references.pmid: 9038336
references.citation: Genetica. 2000;109(1-2):35-44
references.pmid: 11293793
references.citation: J Biol Chem. 2001 Aug 24;276(34):31483-6
references.pmid: 11445559
references.citation: EMBO J. 2002 Mar 1;21(5):1084-91
references.pmid: 11867536
references.citation: Nat Rev Neurosci. 2007 Nov;8(11):832-43
references.pmid: 17925812
references.citation: Development. 2006 Nov;133(22):4475-83
references.pmid: 17035293
references.citation: Genetics. 2009 Jun;182(2):481-91
references.pmid: 19307603
references.citation: Genes Dev. 1995 May 15;9(10):1263-77
references.pmid: 7758950
references.citation: Cytogenet Cell Genet. 1998;80(1-4):142-8
references.pmid: 9678349
references.citation: Cell. 2008 Aug 22;134(4):599-609
references.pmid: 18724933
references.citation: Genes Dev. 2006 Apr 1;20(7):848-57
references.pmid: 16547173
references.citation: Curr Biol. 1998 Nov 19;8(23):1273-6
references.pmid: 9822579
references.citation: Genetics. 2006 Nov;174(3):1151-9
references.pmid: 16951071
references.citation: Genetics. 1990 May;125(1):115-27
references.pmid: 2111262
references.citation: Mech Dev. 2005 Oct;122(10):1094-105
references.pmid: 16125915
references.citation: Nat Rev Genet. 2004 Feb;5(2):114-22
references.pmid: 14735122
references.citation: Annu Rev Genet. 1983;17:345-93
references.pmid: 6421227
references.citation: Nature. 2000 Sep 21;407(6802):405-9
references.pmid: 11014199
references.citation: Genetics. 2006 Dec;174(4):1859-66
references.pmid: 17028315
references.citation: Annu Rev Genet. 1995;29:577-605
references.pmid: 8825487
references.citation: Annu Rev Genet. 2003;37:349-70
references.pmid: 14616065
references.citation: Genome. 2000 Apr;43(2):285-92
references.pmid: 10791816
references.citation: Chromosoma. 2007 Apr;116(2):95-106
references.pmid: 17124606
references.citation: Cell. 1986 Apr 11;45(1):127-36
references.pmid: 3955655
references.citation: J Cell Sci. 2005 Nov 1;118(Pt 21):5047-57
references.pmid: 16234327