Alkylation of adenosine deaminase and thioredoxin by acrylamide in human cell cultures.
Acrylamide is an alpha,beta-unsaturated vinyl monomer that causes cytotoxicity due to its alkylating properties. In recent years several proteins have been identified that are alkylated by acrylamide in vivo. This finding might explain the neurotoxic effects of acrylamide in humans. However, the list of potential acrylamide target proteins is far from being complete. In particular, the proteins that mediate the cytotoxicity of acrylamide in cell cultures remained unknown. Here we identify two novel acrylamide target proteins in human cell cultures (Jurkat, HepG2 and Caco-2), adenosine deaminase and thioredoxin.
Schwend
Thomas
T
Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany.
Möller
Maren
M
Schabacker
Jens
J
Ruppert
Thomas
T
Wink
Michael
M
D020106:Acrylamide / Q000633:toxicity*; D000243:Adenosine Deaminase / Q000378:metabolism*; D000478:Alkylation; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D013879:Thioredoxins / Q000378:metabolism*
D020106:Acrylamide; D013879:Thioredoxins; D000243:Adenosine Deaminase