title: Peptidylarginine deiminase gene is differentially expressed in freshwater and brackish water rainbow trout.
abstract: Peptidylarginine deiminase (PADI)-like cDNA sequence was isolated from rainbow trout (Oncorhynchus mykiss). It consists of a 111-bp 5'-untranslated region, a 731-bp 3'-UTR, and a 2,010-bp open reading frame encoding a protein of 669 amino acids. In the presence of calcium ions, PADI enzymes catalyze the post-translational modification reaction generating citrulline residues. Mammalian PADI enzymes are involved in a number of regulatory processes during cell differentiation and development such as skin keratinization, myelin maturation, and histone deimination. Though five PADI isotypes have been isolated from mammals, in bony fish only one PADI enzyme is present, which contains conserved amino acid residues responsible for catalysis and calcium ion-binding. Sequence identity of piscine PADI protein sequences available at gene databases exceeds 67%. Phylogenetic analyses revealed that not only piscine, but also amphibian and avian PADI-like proteins share most identical amino acid residues with mammalian PADI2. mRNA level of trout PADI-like gene is high in skin, fin, gills, brain, and spleen of rainbow trout. Quantitative Real-Time RT-PCR revealed that PADI gene is differentially expressed in liver, trunk kidney, and spleen of two trout strains, the freshwater-cultured STEELHEAD trout and the brackish water strain BORN.
authors.affiliation: Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany. rebl@fbn-dummerstorf.de
authors.forename: Alexander
authors.initials: A
authors.lastname: Rebl
authors.forename: Bernd
authors.initials: B
authors.lastname: Köllner
authors.forename: Eckhard
authors.initials: E
authors.lastname: Anders
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.forename: Tom
authors.initials: T
authors.lastname: Goldammer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005618:Fresh Water; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006867:Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D000076342:Protein-Arginine Deiminases; D012623:Seawater
chemical_list: D018076:DNA, Complementary; D006867:Hydrolases; D000076342:Protein-Arginine Deiminases
references.citation: J Neurochem. 2002 Apr;81(2):335-43
references.pmid: 12064481
references.citation: Apoptosis. 2007 Mar;12(3):475-87
references.pmid: 17216583
references.citation: J Mol Biol. 2004 Jul 16;340(4):783-95
references.pmid: 15223320
references.citation: J Invest Dermatol. 2005 Feb;124(2):384-93
references.pmid: 15675958
references.citation: Brief Bioinform. 2004 Jun;5(2):150-63
references.pmid: 15260895
references.citation: Biochim Biophys Acta. 1998 Jul 28;1386(1):227-32
references.pmid: 9675292
references.citation: Gene. 2004 Apr 14;330:19-27
references.pmid: 15087120
references.citation: Arthritis Res. 2000;2(4):249-51
references.pmid: 11094435
references.citation: J Exp Med. 2008 Sep 1;205(9):2085-97
references.pmid: 18710930
references.citation: Bioessays. 2003 Nov;25(11):1106-18
references.pmid: 14579251
references.citation: Eur J Biochem. 1999 Feb;259(3):660-9
references.pmid: 10092850
references.citation: J Biomol Struct Dyn. 2008 Oct;26(2):197-201
references.pmid: 18597541
references.citation: J Mol Biol. 1994 Sep 30;242(4):309-20
references.pmid: 7932691
references.citation: Dev Comp Immunol. 2007;31(5):499-510
references.pmid: 17070576
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Biochim Biophys Acta. 1988 Sep 8;966(3):375-83
references.pmid: 3416014
references.citation: Fish Shellfish Immunol. 2008 Jul;25(1-2):153-62
references.pmid: 18502148
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Methods Mol Biol. 1999;112:531-52
references.pmid: 10027275
references.citation: Nat Struct Mol Biol. 2004 Aug;11(8):777-83
references.pmid: 15247907
references.citation: Blood. 2008 Oct 1;112(7):2648-56
references.pmid: 18645041