Proteomic analysis of cold stress-responsive proteins in Thellungiella rosette leaves.
Low temperature is one of the most severe environmental factors that impair plant growth and agricultural production. To investigate how Thellungiella halophila, an Arabidopsis-like extremophile, adapts to cold stress, a comparative proteomic approach based on two-dimensional electrophoresis was adopted to identify proteins that changed in abundance in Thellungiella rosette leaves during short term (6 h, 2 and 5 days) and long term (24 days) exposure to cold stress. Sixty-six protein spots exhibited significant change at least at one time point and maximal cold stress induced-proteome change was found in long-term cold stress group while the minimal change was found in 6-h cold treatment group. Fifty protein spots were identified by mass spectrometry analysis. The identified proteins mainly participate in photosynthesis, RNA metabolism, defense response, energy pathway, protein synthesis, folding and degradation, cell wall and cytoskeleton and signal transduction. These proteins might work cooperatively to establish a new homeostasis under cold stress. Nearly half of the identified cold-responsive proteins were associated with various aspects of chloroplast physiology suggesting that the cold stress tolerance of T. halophila is achieved, at least partly, by regulation of chloroplast function. All protein spots involved in RNA metabolism, defense response, protein synthesis, folding and degradation were found to be upregulated markedly by cold treatment, indicating enhanced RNA metabolism, defense and protein metabolism may play crucial roles in cold tolerance mechanism in T. halophila.
Gao
Fei
F
Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, 100875 Beijing, China.
Zhou
Yijun
Y
Zhu
Weiping
W
Li
Xiaofeng
X
Fan
Liumin
L
Zhang
Genfa
G
D019607:Brassicaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003080:Cold Temperature; D004573:Electrolytes / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D013997:Time Factors
D004573:Electrolytes; D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger
Appl Biochem Biotechnol. 2005 Mar;120(3):169-74
15767691
J Photochem Photobiol B. 2006 Aug 1;84(2):150-60
16624568
Mol Cell Proteomics. 2006 Mar;5(3):484-96
16316980
Plant Cell Environ. 2007 May;30(5):529-38
17407531
Plant Physiol. 1997 Sep;115(1):171-180
12223798
Curr Top Microbiol Immunol. 2008;326:235-55
18630756
Plant Mol Biol. 2007 May;64(1-2):205-17
17318317
Plant Physiol. 2006 Apr;140(4):1437-50
16500996
Plant Cell Environ. 2006 Jul;29(7):1220-34
17080945
Nat Biotechnol. 1999 Feb;17(2):192-6
10052358
Plant Mol Biol. 2006 Mar;60(4):507-18
16525888
Electrophoresis. 2001 Aug;22(13):2824-31
11545414
Proteomics. 2003 Feb;3(2):175-83
12601810
Plant J. 2003 Oct;36(2):141-54
14535880
Plant Cell Physiol. 2007 Dec;48(12):1713-23
17956860
Plant Cell. 2002 Mar;14(3):559-74
11910004
Electrophoresis. 2000 Jul;21(12):2487-91
10939462
J Biol Chem. 2004 Feb 20;279(8):6401-13
14623884
Plant Cell. 2002 Aug;14(8):1675-90
12172015
Plant J. 2008 Aug;55(3):455-66
18410480
J Mol Biol. 2000 Jul 21;300(4):1005-16
10891285
Proteomics. 2005 Jan;5(1):235-44
15672456
Curr Opin Plant Biol. 2007 Jun;10(3):290-5
17468037
Plant Physiol. 2001 Dec;127(4):1354-60
11743073
Proteomics. 2005 Aug;5(12):3162-72
16078185
Plant Physiol. 2003 Aug;132(4):2045-57
12913160
Proteomics. 2002 Sep;2(9):1131-45
12362332
J Exp Bot. 2006;57(7):1537-46
16574749
Plant J. 2006 Sep;47(5):720-34
16923014
Arch Biochem Biophys. 2003 Jun 15;414(2):141-9
12781765
Plant J. 2003 Dec;36(5):652-63
14617066
Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1517-29
11128005
Plant Physiol. 2005 Feb;137(2):762-78
15684019
J Plant Physiol. 2007 Apr;164(4):375-84
17074409
Plant Physiol. 2000 Nov;124(3):941-8
11080272
Proteomics. 2007 Apr;7(8):1293-302
17380535
Nucleic Acids Res. 2009 Jan;37(Database issue):D969-74
18832363
Biochem Biophys Res Commun. 2004 Jul 23;320(2):523-30
15219860
Proteomics. 2007 Apr;7(7):1121-30
17352425
Plant J. 1997 Jul;12(1):179-90
9263459
Nature. 2007 May 17;447(7142):284-8
17450127
Methods. 2001 Dec;25(4):402-8
11846609