title: Loss of Toll-like receptor 2 and 4 leads to differential induction of endoplasmic reticulum stress and proapoptotic responses in the intestinal epithelium under conditions of chronic inflammation.
abstract: Toll-like receptors (TLRs) play an important role in the recognition of microbial molecular patterns of infectious and commensal bacteria and their expression in various tissues including the intestinal epithelium orchestration of the innate and adaptive immune defense mechanisms. Changes in the TLR signaling pathways due to host genetic predispositions may turn a physiological response into a pathological situation including failure of bacterial clearance and development of chronic inflammation. The aim of this study was to characterize the role of TLR2 or TLR4 deficiency in epithelial cell stress responses under noninflamed and inflamed conditions using TLR-deficient mice and TLR(-/-) cross-bred IL-10-deficient mice as a model for genetically driven experimental colitis. Primary intestinal epithelial cells (IEC) were isolated from specific-pathogen-free wild-type, TLR2-, TLR4-, IL-10-, IL-10XTLR2- and IL-10XTLR4-deficient mice at the age of 1, 8, and 16 weeks. Histopathological analysis showed absence of tissue pathology (score 0-12) in distal colon sections of TLR2- and TLR4-deficient mice. In addition, TLR2- but not TLR4-deficient mice cross-bred to the IL-10-deficient background develop moderate colitis, suggesting different effects of these pattern recognition receptors in regulating disease mechanisms. Proteome analysis revealed significantly regulated proteins associated with endoplasmic reticulum (ER) and mitochondrial stress responses in the epithelium. In contrast to TLR2(-/-) and IL-10XTLR2(-/-) mice, the induction of the ER-associated chaperone grp-78 was dissociated from the activation of proapoptotic caspase 3 cleavage in noninflamed TLR4(-/-) and IL10XTLR4(-/-) mice. These results suggest that ER-associated cellular stress responses play an important role in epithelial cells homeostasis leading to beneficial but also deleterious effects. We hypothesize that ER stress-associated processes in the absence of TLR2 and TLR4 differentially affect host responses and epithelial functions under conditions of genetically driven chronic intestinal inflammation.
authors.affiliation: Chair for Biofunctionality, CDD-Center for Diet and Disease, ZIEL-Research Center for Nutrition and Food Science, Technische Universität München, Am Forum 5, Freising-Weihenstephan, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Messlik
authors.forename: Silke
authors.initials: S
authors.lastname: Schmechel
authors.forename: Sigrid
authors.initials: S
authors.lastname: Kisling
authors.forename: Stefan
authors.initials: S
authors.lastname: Bereswill
authors.forename: Markus M
authors.initials: MM
authors.lastname: Heimesaat
authors.forename: Andre
authors.initials: A
authors.lastname: Fischer
authors.forename: Ulf
authors.initials: U
authors.lastname: Göbel
authors.forename: Dirk
authors.initials: D
authors.lastname: Haller
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D002908:Chronic Disease; D003092:Colitis / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006651:Histocytochemistry; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
chemical_list: C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D016753:Interleukin-10