title: Bovine serum albumin promotes IL-1beta and TNF-alpha secretion by N9 microglial cells.
abstract: Bovine serum albumin (BSA) is generally used in biomedical experiments. In the solution of some reagents, BSA is necessary to maintain the stability and concentration of the effective component. Therefore, the potential impact of BSA on experimental results should not be neglected when BSA is used. In this study, we observed that BSA induced significant upregulation of mRNA expression and release of pro-inflammatory cytokines, IL-1beta, and TNF-alpha, by N9 microglial cells. Our results suggest that the effects of BSA should be taken into account in experiments on microglia or the central nervous system when BSA is used. In light of the high similarity and homology among mammalian albumins, our findings also indicate that serum albumin may be a potent trigger of cytokine release by microglia.
authors.affiliation: Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing, 400038, China.
authors.forename: Tian-zhi
authors.initials: TZ
authors.lastname: Zhao
authors.forename: Yong-zhi
authors.initials: YZ
authors.lastname: Xia
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Zhu
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
authors.forename: Jiang-kai
authors.initials: JK
authors.lastname: Lin
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D012710:Serum Albumin, Bovine
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