title: The TOM complex is involved in the release of superoxide anion from mitochondria.
abstract: Available data indicate that superoxide anion (O(2)(*-) ) is released from mitochondria, but apart from VDAC (voltage dependent anion channel), the proteins involved in its transport across the mitochondrial outer membrane still remain elusive. Using mitochondria of the yeast Saccharomyces cerevisiae mutant depleted of VDAC (Deltapor1 mutant) and the isogenic wild type, we studied the role of the TOM complex (translocase of the outer membrane) in the efflux of O(2)(*-) from the mitochondria. We found that blocking the TOM complex with the fusion protein pb(2)-DHFR decreased O(2)(*-) release, particularly in the case of Deltapor1 mitochondria. We also observed that the effect of the TOM complex blockage on O(2)(*-) release from mitochondria coincided with the levels of O(2)(*-) release as well as with levels of Tom40 expression in the mitochondria. Thus, we conclude that the TOM complex participates in O(2)(*-) release from mitochondria.
authors.affiliation: Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Budzińska
authors.forename: Hanna
authors.initials: H
authors.lastname: Gałgańska
authors.forename: Andonis
authors.initials: A
authors.lastname: Karachitos
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wojtkowska
authors.forename: Hanna
authors.initials: H
authors.lastname: Kmita
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D015640:Ion Channel Gating / Q000502:physiology*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D029701:Saccharomyces cerevisiae Proteins; D013481:Superoxides
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