[Effects of ethyl pyruvate on injuries of sepsis in mice].
OBJECTIVE
To explore the effect of ethyl pyruvate (EP) and alkaline phosphatase (ALP) on injuries of sepsis and the mechanism involved.


METHODS
A murine sepsis model of cecal ligation and puncture was reproduced, and 90 male Kunming mice were divided randomly into sham-operation, model and EP-intervention groups. 75 mg/kg EP was intraperitoneally injected in EP groups 1 hour after establishment of model, and the mice in model group were given a same volume of Ringer's solution. The eyeballs were removed in the latter two groups, and mice were sacrificed at 15 minutes and 1, 3 and 6 hours in subgroups of 10 mice each. ALP, uric acid (UA) and ratio of lactic acid and pyruvic acid were determined in serum and homogenized lung tissue by autonomous biochemical analyzer, and pathological changes in intestine were observed by hematoxylin-eosin (HE) staining.


RESULTS
Compared with sham-operation group, serum ALP in model groups and EP groups decreased significantly (P<0.05 or P<0.01), and ALP level of EP group was significantly lower than model group at 6 hours after injury (P<0.05). Compared with sham-operation group, serum UA in model group increased significantly at 1 hour, and reached the highest level at 3 hours (both P<0.05) but decreased significantly later. UA in EP group was significantly lower than that in model group at 1 hour and 3 hours (both P<0.05). Lactic acid/pyruvic acid ratio in lung homogenate of EP group was significantly lower than that of the model group at all the time points (all P<0.05). Intestinal structural damages were distinctly improved in EP group compared with model group at 3 hours and 6 hours (both P<0.05 ).


CONCLUSIONS
EP promotes the utilization of serum ALP, decreases serum UA, ameliorates acidosis and intestinal damages, thus exerting a protective effect on sepsis-induced organ injuries.
Deng
Zi-hui
ZH
Research Laboratory of Biochemistry, Institute of Basic Medicine, General Hospital of PLA, Beijing 100853, China.
Ti
Dong-dong
DD
Xue
Hui
H
Lin
Ji
J
Wang
Lu-huan
LH
Yan
Guang-tao
GT
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D007422:Intestines / Q000187:drug effects / Q000473:pathology; D019344:Lactic Acid / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D011773:Pyruvates / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000378:metabolism; D018805:Sepsis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D014527:Uric Acid / Q000097:blood
D011773:Pyruvates; C046522:ethyl pyruvate; D014527:Uric Acid; D019344:Lactic Acid; D019289:Pyruvic Acid; D000469:Alkaline Phosphatase