title: Expression and prognostic significance of THBS1, Cyr61 and CTGF in esophageal squamous cell carcinoma.
abstract: BACKGROUND
  Thrombospondin1 (THBS1), cystene-rich protein 61 (Cyr61) and connective tissue growth factor (CTGF) are all involved in the transforming growth factor-beta (TGF-beta) signal pathway, which plays an important role in the tumorigenesis. The purpose of this study is to explore the expression and prognostic significance of these proteins in esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  We used immunohistochemistry and western blotting to examine the expression status of THBS1, Cyr61 and CTGF in ESCC. Correlations of THBS1, Cyr61 and CTGF over-expressions with various clinicopathologic factors were also determined by using the Chi-square test or Fisher's exact probability test. Survival analysis was assessed by the Kaplan-Meier analysis and the log-rank test. Relative risk was evaluated by the multivariate Cox proportional hazards model.
  
  
  RESULTS
  THBS1, Cyr61 and CTGF were all over-expressed in ESCC. THBS1 over-expression was significantly associated with TNM stage (P = 0.029) and regional lymph node involvement (P = 0.026). Kaplan-Meier survival analysis showed that over-expression of THBS1, Cyr61 or CTGF was related to poor survival of ESCC patients (P = 0.042, P = 0.020, P = 0.018, respectively). Multivariate Cox analysis demonstrated that Cyr61 and CTGF were independent factors in prognosis of ESCC.
  
  
  CONCLUSIONS
  Cyr61, CTGF and THBS1 were all over-expressed in ESCC and might be new molecular markers to predict the prognosis of ESCC patients.
authors.affiliation: Department of Biochemistry and Molecular Biology, Shantou University, Shantou, PR China. s_zqzhou@163.com
authors.forename: Zhu-Qing
authors.initials: ZQ
authors.lastname: Zhou
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Cao
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Shen
authors.forename: Qing-Ying
authors.initials: QY
authors.lastname: Zhang
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Shen
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism; D055515:Cysteine-Rich Protein 61 / Q000235:genetics* / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D019700:Thrombospondin 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C501848:CCN1 protein, human; C526317:CCN2 protein, human; D055515:Cysteine-Rich Protein 61; D019700:Thrombospondin 1; D055513:Connective Tissue Growth Factor
references.citation: Int J Oncol. 1999 Sep;15(3):453-7
references.pmid: 10427124
references.citation: Cell Mol Life Sci. 2008 Mar;65(5):700-12
references.pmid: 18193162
references.citation: Gynecol Oncol. 2009 Jan;112(1):229-34
references.pmid: 19007976
references.citation: BJU Int. 2008 Sep;102(6):747-55
references.pmid: 18336597
references.citation: Arch Pathol Lab Med. 2000 Feb;124(2):228-33
references.pmid: 10656731
references.citation: Biochim Biophys Acta. 2007 Jan;1775(1):21-62
references.pmid: 16904831
references.citation: Cancer Lett. 2007 Feb 8;246(1-2):230-6
references.pmid: 16616811
references.citation: Cancer Cell Int. 2003 Aug 22;3(1):15
references.pmid: 12969515
references.citation: Surg Today. 2005;35(1):7-18
references.pmid: 15622457
references.citation: Gene. 2001 Aug 22;274(1-2):139-49
references.pmid: 11675006
references.citation: Cancer Res. 2006 Oct 1;66(19):9583-90
references.pmid: 17018615
references.citation: J Histochem Cytochem. 2008 Feb;56(2):193-9
references.pmid: 17998567
references.citation: Clin Cancer Res. 2005 Jan 15;11(2 Pt 2):937s-43s
references.pmid: 15701890
references.citation: J Cell Sci. 2003 Jan 15;116(Pt 2):217-24
references.pmid: 12482908
references.citation: Cancer Res. 2004 Mar 15;64(6):1987-96
references.pmid: 15026334
references.citation: Int J Cancer. 2008 Jul 1;123(1):14-21
references.pmid: 18425817
references.citation: Int J Oncol. 2002 Jun;20(6):1241-6
references.pmid: 12012005
references.citation: Mol Cell Biol. 2008 Jun;28(12):4142-51
references.pmid: 18426913
references.citation: J Clin Oncol. 2005 Mar 20;23(9):2078-93
references.pmid: 15774796
references.citation: J Biol Chem. 2007 Dec 14;282(50):36571-81
references.pmid: 17951630
references.citation: Biomarkers. 2006 Nov-Dec;11(6):547-61
references.pmid: 17056474
references.citation: World J Surg. 2002 Apr;26(4):420-7
references.pmid: 11910473
references.citation: Front Biosci. 2005 May 01;10:998-1009
references.pmid: 15769600
references.citation: N Engl J Med. 2003 Dec 4;349(23):2241-52
references.pmid: 14657432
references.citation: Cancer Res. 2003 Mar 1;63(5):1083-92
references.pmid: 12615726
references.citation: Anticancer Res. 1999 Sep-Oct;19(5C):4375-8
references.pmid: 10650779
references.citation: Cancer Lett. 2007 Aug 28;254(1):42-53
references.pmid: 17374440
references.citation: J Biol Chem. 2001 Apr 6;276(14):10594-601
references.pmid: 11152469
references.citation: Cell. 1998 Jun 26;93(7):1159-70
references.pmid: 9657149
references.citation: Oncogene. 2002 Jan 31;21(6):964-73
references.pmid: 11840342
references.citation: PLoS One. 2007 Jun 20;2(6):e534
references.pmid: 17579708
references.citation: J Natl Cancer Inst. 1997 Feb 5;89(3):219-27
references.pmid: 9017002
references.citation: Exp Biol Med (Maywood). 2001 Sep;226(8):726-33
references.pmid: 11520937
references.citation: J Biol Chem. 2001 Dec 14;276(50):47709-14
references.pmid: 11598125