title: HIV type 1 diversity from newly diagnosed patients in Santos metropolitan area/Brazil.
abstract: HIV-1 from infected subjects has been characterized in order to provide a more accurate view of the strains that are currently found in a given region. In this report, we focused on characterizing the pol gene diversity obtained from newly diagnosed patients in Santos metropolitan area, Brazil. This region is composed of nine cities and an international port. Analysis of the 33 samples revealed that 22 strains belonged to subtype B, 4 to subtype F, and 2 to subtype C; 5 strains were B/F recombinants. Our results demonstrated that 18.2% of samples were primary antiretroviral resistance genotypic mutations, with high-level resistance to reverse transcriptase inhibitors in both subtypes B and F and in recombinant forms B/F. Our data revealed that the primary antiretroviral resistance genotypic mutations should be carefully investigated in developing countries with widespread access to antiretrovirals, such as Brazil.
authors.affiliation: Centro Universitário Lusíada (UNILUS) , Núcleo Acadêmico de Estudos e Pesquisas em Virologia/Laboratório de Biologia Molecular, Boqueirão, Santos SP, Brazil 11045-101. dercy.sa@unifesp.br
authors.forename: Dercy José
authors.initials: DJ
authors.lastname: de Sa-Filho
authors.forename: Rafael Favero
authors.initials: RF
authors.lastname: Ambar
authors.forename: Natalia Brenneken
authors.initials: NB
authors.lastname: Duarte
authors.forename: Rafael Bragança Rodrigues
authors.initials: RB
authors.lastname: Matias
authors.forename: Valéria
authors.initials: V
authors.lastname: Candido
authors.forename: Luiz Henrique
authors.initials: LH
authors.lastname: Gagliani
authors.forename: Marcos Montani
authors.initials: MM
authors.lastname: Caseiro
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000494:pharmacology; D001938:Brazil; D016000:Cluster Analysis; D024882:Drug Resistance, Viral; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014505:Urban Population; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D054302:pol Gene Products, Human Immunodeficiency Virus