Cytotoxic activities, cellular uptake, gene regulation, and optical imaging of novel platinum(II) complexes.
A new class of platinum(II) coordination complexes and their dye tagged conjugates has been synthesized from N-substituted diaminocyclohexane ligands. The in vitro anticancer activities of the platinum compounds have been validated against the breast cancer cell-line MCF-7 and the normal cell-line MCF-10A via sulforhodamine B and colony formation assay. The platinum compounds and the corresponding metal-free ligands exhibited higher drug efficiencies than cisplatin and oxaliplatin against MCF-7 cells. Cellular uptake and DNA-bound Pt were demonstrated by atomic absorption spectroscopy. The platinum complexes displayed increased cellular accumulation and DNA binding as compared with cisplatin. Real-time reverse transcription polymerase chain reaction assay was employed to investigate drug effects on mRNA expression in MCF-7 cells. The results indicated that the study compounds are effective in regulating cyclin D1, Bcl-2, and p53 genes; yet, oxaliplatin is less effective in manipulating those genes. The luminescent probe that was integrated into the platinum complexes made it possible to monitor cellular drug distribution using optical imaging. Targeting of tumor cell nuclei by the study compounds was confirmed by confocal microscopy. Taken together, these new platinum(II)-based antitumor agents are different from marketed platinum drugs in several critical aspects and could have potential in cancer therapy.
Gao
Jian
J
Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA. gaojiantamu@yahoo.com
Liu
Ya-Guang
YG
Zingaro
Ralph A
RA
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000633:toxicity; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D005786:Gene Expression Regulation; D006801:Humans; D018613:Microscopy, Confocal; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D000077150:Oxaliplatin; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
C546571:2,2'-cyclohexane-1,2-diylbis(azanediyl)bis(methylene)diphenoldichloroplatinum(II); D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D009944:Organoplatinum Compounds; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D000077150:Oxaliplatin; D019938:Cyclin D1; D004247:DNA; D002945:Cisplatin