title: Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation.
abstract: To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding beta-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.
authors.affiliation: Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580-185, Jeonbuk, South Korea.
authors.forename: Lian Hua
authors.initials: LH
authors.lastname: Luo
authors.forename: Pil-Soo
authors.initials: PS
authors.lastname: Seo
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Sun-Yeon
authors.initials: SY
authors.lastname: Heo
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kim
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D054889:Fatty Acid Synthase, Type II; D005227:Fatty Acids / Q000378:metabolism*; D018628:Gene Dosage; D005818:Genetic Engineering; D006836:Hydro-Lyases / Q000235:genetics* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D000431:Ethanol; D006836:Hydro-Lyases; C047118:3-hydroxyacyl-(acyl-carrier-protein) dehydratase; D054889:Fatty Acid Synthase, Type II