Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate.
Goedl
Christiane
C
Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, 8010 Graz, Austria.
Nidetzky
Bernd
B
D019943:Amino Acid Substitution; D020134:Catalytic Domain; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism; D016695:Glycosyltransferases / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D013329:Structure-Activity Relationship
D016695:Glycosyltransferases; D005964:Glucosyltransferases; C025901:sucrose phosphorylase