[Coix lachryma jobi L varma-yuan induces apoptosis of jurkat cell line in acute T lymphoblast leukemia and its mechanism].
The aim of the present study was to investigate the anti-proliferation and pro-apoptosis effect of Coix lachrymajobi L varma-yuan on acute T lymphoblast leukemia cell line Jurkat cells and its mechanism. Jurkat cells were treated with Coix lachrymajobi L varma-yuan of various concentrations (0, 0.4, 0.8, 1.6 mg/ml) for 24h. The inhibitory ratio was measured by Cell Counting Kit-8. The effects of Coix lachrymajobi L varma-yuan on apoptosis of Jurkat cells were determined by Hoechst 33258, PI and Annexin V-FITC/PI double staining. The mitochondrial membrane potential was analyzed by JC-1 staining. The results demonstrated that Coix lachrymajobi L varma-yuan inhibited the proliferation of Jurkat cells, and induced chromatin condensation and fragmentation (characteristic of apoptosis) and loss of mitochondrial membrane potential. In conclusion, Coix lachrymajobi L varma-yuan can inhibit the cell proliferation and induce the apoptosis of Jurkat cells. These effects relate to loss of mitochondrial membrane potential. These results suggest that Coix lachrymajobi L varma-yuan may be of value in treating lymphoma.
Yao
Gen-Hong
GH
Department of Blood Transfusion, Nanjing General Hospital, Nanjing Military Area of PLA, School of Medicine, Nanjing University, Nanjing 210002, Jiangsu Province, China.
Zhang
Guo-Dong
GD
Luan
Jian-Feng
JF
Ye
Dong
D
Yan
Jin-Mei
JM
Zhu
Pei-Yuan
PY
Lei
Qian-Hong
QH
D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D031742:Coix / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D053078:Membrane Potential, Mitochondrial; D010938:Plant Oils / Q000494:pharmacology*
D010938:Plant Oils