title: Antinociceptive activity and toxicology of the lectin from Canavalia boliviana seeds in mice.
abstract: The aim of the present study was to evaluate the potential antinociceptive and toxicity of Canavalia boliviana lectin (CboL) using different methods in mice. The role of carbohydrate-binding sites was also investigated. CboL given to mice daily for 14 days at doses of 5 mg/kg did not cause any observable toxicity. CboL (1, 5, and 10 mg/kg) administered to mice intravenously inhibited abdominal constrictions induced by acetic acid and the two phases of the formalin test. In the hot plate and tail immersion tests, the same treatment of CboL induced significant increase in the latency period. In the hot plate test, the effect of CboL (5 mg/kg) was reversed by naloxone (1 mg/kg), indicating the involvement of the opioid system. In the open-field and rota-rod tests, the CboL treatment did not alter animals' motor function. These results show that CboL presents antinociceptive effects of both central and peripheral origin, involving the participation of the opioid system via lectin domain.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60451-970, Fortaleza, CE, Brazil.
authors.forename: Jozi Godoy
authors.initials: JG
authors.lastname: Figueiredo
authors.forename: Flávio
authors.initials: F
authors.lastname: da Silveira Bitencourt
authors.forename: Ingrid Gonçalves
authors.initials: IG
authors.lastname: Beserra
authors.forename: Cícero Silvano
authors.initials: CS
authors.lastname: Teixeira
authors.forename: Patrícia Bastos
authors.initials: PB
authors.lastname: Luz
authors.forename: Eduardo Henrique Salviano
authors.initials: EH
authors.lastname: Bezerra
authors.forename: Mário Rogério Lima
authors.initials: MR
authors.lastname: Mota
authors.forename: Ana Maria Sampaio
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Fernando
authors.initials: F
authors.lastname: de Queiroz Cunha
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.forename: Nylane Maria Nunes
authors.initials: NM
authors.lastname: de Alencar
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D032449:Canavalia / Q000737:chemistry*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D037121:Plant Lectins / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D012639:Seeds
chemical_list: D000700:Analgesics; D037121:Plant Lectins; C039268:lectin, Canavalia
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