Gas chromatography/mass spectrometry screening of serum metabolomic biomarkers in hepatitis B virus infected cirrhosis patients.
BACKGROUND
Metabolomic studies have been applied to disease biomarkers selection. With the metabolomic technique, gas chromatography/mass spectrometry (GC/MS), human serum metabolites can be detected and identified. The purpose of this study was to investigate the serum metabolic profile of hepatitis B virus (HBV) infected cirrhosis patients and to detect disease biomarkers.


METHODS
HBV infected non-cirrhosis male subjects (n=20) and HBV infected cirrhosis male patients (n=20) participated in this experiment. Serum metabolome was detected through chemical derivatization followed by GC/MS. The high-flux metabolomic data were analyzed by stepwise discriminant analysis.


RESULTS
Out of the 41 metabolites detected in serum, we selected metabolites, including acetic acid, sorbitol, D-lactic acid, hexanoic acid, 1-naphthalenamine, butanoic acid, phosphoric acid, D-glucitol, and glucose, which in combination with each other could segregate the two groups. The error count was 0% for the non-cirrhosis group and 25% for the cirrhosis group.


CONCLUSIONS
This technique can be used to select biomarkers for hepatic cirrhosis.
Xue
Ruyi
R
Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
Dong
Ling
L
Wu
Hao
H
Liu
Taotao
T
Wang
Jiyao
J
Shen
Xizhong
X
D000328:Adult; D015415:Biomarkers / Q000097:blood* / Q000378:metabolism; D003937:Diagnosis, Differential; D008401:Gas Chromatography-Mass Spectrometry; D006509:Hepatitis B / Q000097:blood* / Q000150:complications / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000150:complications / Q000175:diagnosis / Q000821:virology; D008111:Liver Function Tests; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D012680:Sensitivity and Specificity
D015415:Biomarkers