Characterization of native Bacillus thuringiensis strains and selection of an isolate active against Spodoptera frugiperda and Peridroma saucia.
Twelve Bacillus thuringiensis (Bt) strains, isolated from larvae and soil samples in Argentina, were molecularly and phenotypically characterized and their insecticidal activities against Spodoptera frugiperda and Peridroma saucia were determined. One isolate--Bt RT--produced more than 93% mortality on first instar larvae of both species, which was higher than that produced by the reference strain Bt 4D1. Bt RT carried a different cry gene profile than Bt 4D1. Scanning electron microscopy showed the presence of bipyramidal and cuboidal crystals. Phenotypic characterization revealed lytic enzymes that could contribute to Bt pathogenicity.
Alvarez
Analía
A
Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, Miguel Lillo 205, T4001 MVB, Tucumán, Argentina. alvanalia@gmail.com
Virla
Eduardo G
EG
Pera
Licia M
LM
Baigorí
Mario D
MD
D000818:Animals; D001118:Argentina; D001413:Bacillus thuringiensis / Q000302:isolation & purification* / Q000472:pathogenicity* / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism / Q000633:toxicity* / Q000648:ultrastructure; D004731:Endotoxins / Q000378:metabolism / Q000633:toxicity*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D006460:Hemolysin Proteins / Q000378:metabolism / Q000633:toxicity* / Q000648:ultrastructure; D007814:Larva / Q000382:microbiology; D007915:Lepidoptera / Q000187:drug effects* / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D012988:Soil Microbiology; D018411:Spodoptera / Q000187:drug effects / Q000382:microbiology; D016019:Survival Analysis
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis