Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy.
In this study, the topography of human topoisomerase I (TOPO I) on mica surfaces in air and in liquid has been studied by atomic force microscopy (AFM). The average height of TOPO I on mica surface in air measured by AFM was 2.59+/-0.32 nm. After adsorption of the 0.3 U/microl TOPO I on mica surfaces for 2 h, and then imaged in liquid by AFM, well-separated single TOPO I was observed. The average height of TOPO I on mica surfaces in liquid measured by AFM was 2.93+/-0.42 nm. After adsorption of the 4 U/microl TOPO I on mica surfaces for 1.5 h, TOPO I monolayer can be formed. The produced TOPO I monolayer on mica was flat and exhibited good stability.
Liu
Zhiguo
Z
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin, China.
Meng
Ronghua
R
Zu
Yuangang
Y
Li
Qingyong
Q
Yao
Liping
L
D000327:Adsorption; D000388:Air; D000538:Aluminum Silicates; D004264:DNA Topoisomerases, Type I / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D014867:Water
D000538:Aluminum Silicates; D014867:Water; D004264:DNA Topoisomerases, Type I; C011934:mica