Effect of quercetin on platelet spreading on collagen and fibrinogen and on multiple platelet kinases.
alpha(2)beta(1) and alpha(IIb)beta(3) integrins, that support platelet adhesion to collagen and fibrinogen, respectively, share common signaling molecules. The effect of quercetin on platelet static adhesion to collagen and fibrinogen was assessed and correlated with its kinase inhibitory activity. Quercetin strongly abrogated PI3K and Src kinases, mildly inhibited Akt1/2, and slightly affected PKC, p38 and ERK1/2. Quercetin or the combined use of adenosine diphosphate and thromboxane A(2) inhibitors abrogated platelet spreading on these surfaces to a similar extent. We suggest that the inhibitory effect of quercetin on platelet kinases blocks early signaling events preventing a complete platelet spreading.
Navarro-Núñez
Leyre
L
Unit of Haematology and Medical Oncology, Centro Regional de Hemodonación, University of Murcia, Spain. mleyrenn@um.es
Lozano
María L
ML
Martínez
Constantino
C
Vicente
Vicente
V
Rivera
José
J
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen; D005340:Fibrinogen; D006801:Humans; D016023:Integrins / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000187:drug effects*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013928:Thromboxane A2 / Q000037:antagonists & inhibitors
D016023:Integrins; D047428:Protein Kinase Inhibitors; D013928:Thromboxane A2; D000244:Adenosine Diphosphate; D005340:Fibrinogen; D003094:Collagen; D011794:Quercetin; D011494:Protein Kinases; D019869:Phosphatidylinositol 3-Kinases