Polyelectrolyte multilayers capped with polyelectrolytes bearing phosphorylcholine and triethylene glycol groups: parameters influencing antifouling properties.
In this paper, we investigate the design of antifouling surfaces by the deposition of polyelectrolytes modified by grafting of antifouling groups onto a (PSS/PAH)n precursor multilayer film [PSS, poly(styrenesulfonate); PAH, poly(allylamine)]. Different polyelectrolytes and different antifouling moieties are investigated, in particular, (EO)3 and (EO)3PC moieties (EO, ethylene oxide; PC, phosphorylcholine group). We find that protein adsorption can strongly be reduced and even practically suppressed through the deposition of only one layer of polyelectrolyte modified with PC and/or (EO)3 groups. We discuss the influence of various parameters such as the nature of the polyelectrolyte backbone, the nature of the antifouling moiety, and the grafting ratio on the reduction of protein adsorption. We find in particular that (EO)3 and (EO)3PC moieties grafted on poly(acrylic acid) (PAA) totally prevent protein adsorption for grafting ratios of 25% or more, at least within the detection limits of the used quartz crystal microbalance and optical waveguide light mode spectroscopy devices. The mechanism that leads to the antifouling property is discussed and compared to that leading to the antifouling properties of ethylene oxide self-assembled monolayers. Finally, by incorporating biotin on top of the precursor film, we show that one layer of PAA-(EO)3PC is not sufficient to prevent interaction with streptavidin but a PAA-(EO)3PC/PAH/PAA-(EO)3PC multilayer largely protects the biotin from interacting with streptavidin.
Reisch
Andreas
A
Institut Charles Sadron, CNRS, 23 rue du Loess, BP 84047, 67037 Strasbourg Cedex 2, France.
Voegel
Jean-Claude
JC
Gonthier
Eric
E
Decher
Gero
G
Senger
Bernard
B
Schaaf
Pierre
P
Mésini
Philippe J
PJ
D000327:Adsorption; D001696:Biomechanical Phenomena; D003102:Colloids / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry*; D008956:Models, Chemical; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010767:Phosphorylcholine / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011312:Pressure; D013499:Surface Properties
D003102:Colloids; D004573:Electrolytes; D010767:Phosphorylcholine; C028914:triethylene glycol; D011092:Polyethylene Glycols