title: [Establishment of RT- LAMP for rapid detection of foot-and-mouth disease virus].
abstract: A rapid detection of foot-and-mouth disease virus (FMDV) was established by using reverse transcription loop-mediated isothermal amplification (RT-LAMP) method, meanwhile its specificity and sensitivity were assessed. The results showed that the FMDV RNA could be amplified by incubation at 65degrees C for only 1h using six primers designed based on FMDV polyprotein gene and the amplification products could be detected easily by naked-eye. There is no cross reaction with other virus such as SVDV, SFV and PPV by detecting their RNA samples. The detection limit of this method was found to be 10(-5) dilution of RNA sample which was 100-fold higher than that of PCR and 10-fold higher than real-time PCR.
authors.affiliation: Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai, China. lijiancq@yahoo.com.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Xue-En
authors.initials: XE
authors.lastname: Fang
mesh_terms: D000818:Animals; D017931:DNA Primers; D005536:Foot-and-Mouth Disease / Q000517:prevention & control / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013555:Swine Vesicular Disease / Q000821:virology
chemical_list: D017931:DNA Primers; D012367:RNA, Viral