title: Quantitative analysis of patulin in apple juice by thin-layer chromatography using a charge coupled device detector.
abstract: A method was developed and validated in-house for the detection and quantification of patulin in apple juice concentrate using a charge coupled device (CCD) on thin-layer chromatography (TLC) plates. Samples were extracted with ethyl acetate and then cleaned-up by extraction with a sodium carbonate solution. The method showed a mean recovery of 95%. The quantification and detection limit were 14 microg l(-1) and 0.005 microg per spot, respectively. The CCD camera is sufficiently sensitive to detect changes in spot fluorescence intensity caused by small differences in mycotoxin concentration under homogeneous illumination from a UV light source. The results of validation confirmed the efficiency of the method, which is sensitive enough to be used to quantify patulin in apple juice by producers or for government monitoring/survey programs. The method was applied to the analysis of 16 apple juice concentrate samples and patulin levels ranged from 15 to 46 microg l(-1). This study demonstrated the applicability of the TLC-CCD technique as a tool for monitoring patulin in apple juice.
authors.affiliation: Institute of Food Science and Technology, Federal University of Rio Grande do Sul, 91570-901 Porto Alegre, RS, Brazil. juliwelke@yahoo.com.br
authors.forename: Juliane Elisa
authors.initials: JE
authors.lastname: Welke
authors.forename: Michele
authors.initials: M
authors.lastname: Hoeltz
authors.forename: Horacio Alberto
authors.initials: HA
authors.lastname: Dottori
authors.forename: Isa Beatriz
authors.initials: IB
authors.lastname: Noll
mesh_terms: D001628:Beverages / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D010365:Patulin