title: Photoregulation of thrombin aptamer activity using Bhc caging strategy.
abstract: Thrombin aptamer was attempted to cage with Bhc (6-bromo-7-hydroxycoumarin-4-ylmethyl) group for controlling its specific affinity to target molecular through photolysis. By multiple-caging strategy, aptamer could be rendered biologically inert and partially restored with subsequently illumination. This provides a convenient method for photoregulating aptamer activity with exact spatiotemporal resolution.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Luo
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Chen
authors.forename: Fengliang
authors.initials: F
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Wu
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Guo
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D008027:Light; D010782:Photolysis; D011485:Protein Binding; D013917:Thrombin / Q000378:metabolism; D014468:Umbelliferones / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D014468:Umbelliferones; C081676:thrombin aptamer; C031477:7-hydroxycoumarin; D013917:Thrombin