title: Polarized expression of integrin beta1 in diencephalic roof plate during chick development, a possible receptor for SCO-spondin.
abstract: The roof plate of the caudal diencephalon is formed by the posterior commissure (PC) and the underlying secretory ependyma, the subcommissural organ (SCO). The SCO is composed by radial glial cells bearing processes that cross the PC and attach to the meningeal basement membrane. Since early development, the SCO synthesizes SCO-spondin, a glycoprotein that shares similarities to axonal guidance proteins. In vitro, SCO-spondin promotes neuritic outgrowth through a mechanism mediated by integrin beta1. However, the secretion of SCO-spondin toward the extracellular matrix that surrounds the PC axons and the expression of integrins throughout PC development have not been addressed. Here we provide immunohistochemical evidence to suggest that during chick development SCO cells secrete SCO-spondin through their basal domain, where it is deposited into the extracellular matrix in close contact with axons of the PC that express integrin beta1. Our results suggest that SCO-spondin has a role in the development of the PC through its interaction with integrin beta1.
authors.affiliation: Department of Cell Biology, University of Concepción, Chile.
authors.forename: Teresa
authors.initials: T
authors.lastname: Caprile
authors.forename: Germán
authors.initials: G
authors.lastname: Osorio
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Henríquez
authors.forename: Hernán
authors.initials: H
authors.lastname: Montecinos
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D004027:Diencephalon / Q000033:anatomy & histology / Q000196:embryology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D039503:Integrin alpha6 / Q000235:genetics / Q000378:metabolism; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis / Q000502:physiology; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D013351:Subcommissural Organ / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D039503:Integrin alpha6; D019012:Integrin beta1; D019006:Neural Cell Adhesion Molecules; C108402:SCO-spondin; D014746:Vimentin