[Effects of shexiang baoxin pill on function and nitric oxide secretion of endothelial progenitor cells].
OBJECTIVE
To investigate the effects of Shexiang Baoxin Pill (SBP) on function of endothelial progenitor cells (EPCs) and its nitric oxide (NO) secretion.


METHODS
Total mononuclear cells were isolated from human peripheral blood by ficoll density gradient centrifugation and inoculated on the human fibro-ligandin encrusting plate. After 7 days of in vitro culture, adherent cells were collected and incubated with SBP for 24 h. The proliferation, migration, adhesive activity, vasculogenesis capacity and NO secretion of EPCs were assayed using MTT, Transwell chamber, adhesion determination, in vitro vasculogenesis kit and nitrate reductase method, respectively.


RESULTS
EPCs incubated with SBP showed the capacities higher than those of control in proliferation, migration, adhesion, in vitro vasculogenesis, and with a higher NO concentration in the culture supernatant.


CONCLUSIONS
SBP can improve the function of EPCs, which might be a novel mechanism of its effects in improving vascular endothelial function and promoting angiogenesis.
Wu
Jian-Xiang
JX
Department of Cardiology, Second Affiliated Hospital of Second Military Medical University, Shanghai.
Liang
Chun
C
Ren
Yu-Sheng
YS
D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D009569:Nitric Oxide / Q000096:biosynthesis*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology
D004365:Drugs, Chinese Herbal; C526712:shexiang baoxin; D009569:Nitric Oxide