title: Molecular diagnosis and treatment monitoring of congenital transmission of Trypanosoma cruzi to twins of a triplet delivery.
abstract: Congenital transmission of Trypanosoma cruzi was diagnosed in 2 triplets born to a triamniotic bichorionic delivery. Only the sisters sharing the placenta became infected, as diagnosed by microhematocrit and/or polymerase chain reaction of 3 parasite targets. The neonates' parasitologic response to benznidazole was monitored. Molecular strategies allowed genotyping lineage IId and identical minicircle signatures in both triplets, showing they harbored the same maternal parasite populations.
authors.affiliation: Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires C1428ADL, Argentina.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Burgos
authors.forename: Jaime
authors.initials: J
authors.lastname: Altcheh
authors.forename: Noemi
authors.initials: N
authors.lastname: Petrucelli
authors.forename: Margarita
authors.initials: M
authors.lastname: Bisio
authors.forename: Mariano J
authors.initials: MJ
authors.lastname: Levin
authors.forename: Hector
authors.initials: H
authors.lastname: Freilij
authors.forename: Alejandro G
authors.initials: AG
authors.lastname: Schijman
mesh_terms: D000293:Adolescent; D000818:Animals; D014355:Chagas Disease / Q000175:diagnosis / Q000188:drug therapy* / Q000635:transmission*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D009593:Nitroimidazoles / Q000627:therapeutic use; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis* / Q000188:drug therapy*; D017422:Sequence Analysis, DNA; D014310:Triplets; D014344:Trypanocidal Agents / Q000627:therapeutic use; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan; D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C009999:benzonidazole