title: Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.).
abstract: RNA-dependent RNA polymerases (RdRPs) in plants have been reported to be involved in post-transcriptional gene silencing (PTGS) and antiviral defense. In this report, an RdRP gene from maize (ZmRdRP1) was obtained by rapid amplification of cDNA ends (RACE) and RT-PCR. The mRNA of ZmRdRP1 was composed of 3785 nucleotides, including a 167 nt 5' untranslated region (UTR), a 291 nt 3'UTR and a 3327 nt open reading frame (ORF), which encodes a putative protein of 1108 amino acids with an estimated molecular mass of 126.9 kDa and a predicated isoelectric point (pI) of 8.37. Real-time quantitative RT-PCR analysis showed that ZmRdRP1 was elicited by salicylic acid (SA) treatment, methyl jasmonate (MeJA) treatment and sugarcane mosaic virus (SCMV) infection. We silenced ZmRdRP1 by constitutively expressing an inverted-repeat fragment of ZmRdRP1 (ir-RdRP1) in transgenic maize plants. Further studies revealed that the ir-RdRP1 transgenic plants were more susceptible to SCMV infection than wild type plants. Virus-infected transgenic maize plants developed more serious disease symptoms and accumulated more virus than wild type plants. These findings suggested that ZmRdRP1 was involved in antiviral defense in maize.
authors.affiliation: State Key Laboratory of Agrobiotecnology, College of Biology Science, China Agricultural University, 100193, Beijing, China.
authors.forename: Junguang
authors.initials: J
authors.lastname: He
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Dong
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Jia
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Guoying
authors.initials: G
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018390:Gene Targeting; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000502:physiology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology; D030821:Plants, Genetically Modified; D034622:RNA Interference; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D019562:Viral Load / Q000235:genetics; D003313:Zea mays / Q000235:genetics* / Q000276:immunology / Q000502:physiology / Q000821:virology
chemical_list: D012324:RNA-Dependent RNA Polymerase
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