The activation of matrix metalloproteinase-2 induced by protein kinase C alpha in decidualization.
This study investigated the protein kinase C (PKC) and matrix metalloproteinase-2 (MMP-2) in the development of deciduomata in pseudo-pregnant and pregnant rats. The results showed that the expression of MMP-2 was significantly increased from day 2 to day 5 in pseudo-pregnancy and from day 7 to day 9 in pregnancy. To further investigate the correlation between MMP-2 and protein kinase C alpha (PKC alpha), the expression of MMP-2 in the 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated organotypic culture of decidual tissue was determined. The results showed that the active form of MMP-2 was significantly increased in the TPA-treated cultures. Moreover, this response was inhibited by the PKC inhibitor H7, the PKC alpha specific inhibitor Gö-6976 and the translation inhibitor cycloheximide, but not by the transcription inhibitor actinomycin D or the replication inhibitor mitomycin C. In addition, TPA also reversed the MMP-2 expression after by progesterone pretreatment in the primary decidual cells. These findings indicate that PKC alpha may play an important role in the regulation of the MMP-2 expression during decidualization.
Tsai
Jen-Hsiang
JH
Department of Physical Therapy, School of Medical and Health Sciences, Fooyin University, Kaohsiung 83102, Taiwan.
Hwang
Jin-Ming
JM
Ying
Tsung-Ho
TH
Shyu
Jyh-Cherng
JC
Tsai
Chin-Chiu
CC
Hsieh
Yih-Shou
YS
Wang
Yi-Wei
YW
Liu
Jer-Yuh
JY
Kao
Shao-Hsuan
SH
D000818:Animals; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009924:Organ Culture Techniques; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism
D047428:Protein Kinase Inhibitors; D014157:Transcription Factors; D011374:Progesterone; D051571:Protein Kinase C-alpha; D020778:Matrix Metalloproteinase 2; D013755:Tetradecanoylphorbol Acetate