title: [Determination of content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes by RP-HPLC method].
abstract: OBJECTIVE
  To establish a RP-HPLC method for content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes.
  
  
  METHODS
  The separation was performed with a COSMOSIL 5 C18-MS-II column (4.6 mm x 250 mm, 5 mmicrom) using methanol-water (95:5) as the mobile phase and detected at 203 nm. The flow rate was 1.0 mL x min(-1) and 50 microL sample solution was injected for each time.
  
  
  RESULTS
  The calibration curve was linear within the range 0.1-0.5 mg x mL(-1) (r = 0. 9999) , the intra-day RSD and inter-day RSD were less than 2% and the average recovery was between 101.44%-103.11% (n = 3).
  
  
  CONCLUSIONS
  The method is simple, accurate, sensitive and applicable for determination of content and entrapment efficiency of 20 (S)-protopanaxadiol pharmacosomes.
authors.affiliation: Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100193, China. hanmeihua727@163.com
authors.forename: Meihua
authors.initials: M
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Wang
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005894:Panax / Q000737:chemistry*; D015203:Reproducibility of Results; D012502:Sapogenins / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D012502:Sapogenins; C062916:protopanaxadiol