title: Gas chromatography/mass spectrometry screening of serum metabolomic biomarkers in hepatitis B virus infected cirrhosis patients.
abstract: BACKGROUND
  Metabolomic studies have been applied to disease biomarkers selection. With the metabolomic technique, gas chromatography/mass spectrometry (GC/MS), human serum metabolites can be detected and identified. The purpose of this study was to investigate the serum metabolic profile of hepatitis B virus (HBV) infected cirrhosis patients and to detect disease biomarkers.
  
  
  METHODS
  HBV infected non-cirrhosis male subjects (n=20) and HBV infected cirrhosis male patients (n=20) participated in this experiment. Serum metabolome was detected through chemical derivatization followed by GC/MS. The high-flux metabolomic data were analyzed by stepwise discriminant analysis.
  
  
  RESULTS
  Out of the 41 metabolites detected in serum, we selected metabolites, including acetic acid, sorbitol, D-lactic acid, hexanoic acid, 1-naphthalenamine, butanoic acid, phosphoric acid, D-glucitol, and glucose, which in combination with each other could segregate the two groups. The error count was 0% for the non-cirrhosis group and 25% for the cirrhosis group.
  
  
  CONCLUSIONS
  This technique can be used to select biomarkers for hepatic cirrhosis.
authors.affiliation: Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Ruyi
authors.initials: R
authors.lastname: Xue
authors.forename: Ling
authors.initials: L
authors.lastname: Dong
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Taotao
authors.initials: T
authors.lastname: Liu
authors.forename: Jiyao
authors.initials: J
authors.lastname: Wang
authors.forename: Xizhong
authors.initials: X
authors.lastname: Shen
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood* / Q000378:metabolism; D003937:Diagnosis, Differential; D008401:Gas Chromatography-Mass Spectrometry; D006509:Hepatitis B / Q000097:blood* / Q000150:complications / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000150:complications / Q000175:diagnosis / Q000821:virology; D008111:Liver Function Tests; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers