title: Sensitivity test of derivative matrix isopotential synchronous fluorimetry and least squares fitting methods.
abstract: Determination of concentrations of spectrally overlapping compounds has special difficulties. Several methods are available to calculate the constituents' concentrations in moderately complex mixtures. A method which can provide information about spectrally hidden components in mixtures is very useful. Two methods powerful in resolving spectral components are compared in this paper. The first method tested is the Derivative Matrix Isopotential Synchronous Fluorimetry (DMISF). It is based on derivative analysis of MISF spectra, which are constructed using isopotential trajectories in the Excitation-Emission Matrix (EEM) of background solution. For DMISF method, a mathematical routine fitting the 3D data of EEMs was developed. The other method tested uses classical Least Squares Fitting (LSF) algorithm, wherein Rayleigh- and Raman-scattering bands may lead to complications. Both methods give excellent sensitivity and have advantages against each other. Detection limits of DMISF and LSF have been determined at very different concentration and noise levels.
authors.affiliation: Department of Experimental Physics, University of Pécs, Ifjúság u. 6., Pécs, H-7624, Hungary. mgeza@ddkkk.pte.hu
authors.forename: Géza
authors.initials: G
authors.lastname: Makkai
authors.forename: Andrea
authors.initials: A
authors.lastname: Buzády
authors.forename: János
authors.initials: J
authors.lastname: Erostyák
mesh_terms: D000465:Algorithms; D005470:Fluorometry / Q000379:methods* / Q000706:statistics & numerical data*; D016018:Least-Squares Analysis; D057230:Limit of Detection; D016014:Linear Models
references.citation: Anal Biochem. 2001 May 1;292(1):59-68
references.pmid: 11319818
references.citation: Water Res. 2003 Jul;37(13):3055-60
references.pmid: 14509692
references.citation: Talanta. 2007 Mar 15;71(4):1481-6
references.pmid: 19071479
references.citation: Talanta. 2004 Apr 19;62(5):971-6
references.pmid: 18969387
references.citation: J Pharm Biomed Anal. 1998 Jul;17(3):379-86
references.pmid: 9656147
references.citation: Anal Sci. 2001 Jan;17(1):167-70
references.pmid: 11993656
references.citation: Anal Chim Acta. 2007 Jan 30;583(1):55-62
references.pmid: 17386526
references.citation: Clin Chem. 2004 Oct;50(10):1797-803
references.pmid: 15308592
references.citation: Talanta. 1998 Nov;47(3):537-45
references.pmid: 18967356
references.citation: Talanta. 2006 Jul 15;69(5):1265-8
references.pmid: 18970713
references.citation: Chem Pharm Bull (Tokyo). 2006 Jan;54(1):119-22
references.pmid: 16394564
references.citation: Anal Sci. 2005 Oct;21(10):1203-6
references.pmid: 16270579
references.citation: Anal Chim Acta. 2007 May 29;592(1):82-90
references.pmid: 17499074