Survival of Lactobacillus reuteri DSM 17938 and Lactobacillus rhamnosus GG in the human gastrointestinal tract with daily consumption of a low-fat probiotic spread.
Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. Therefore, probiotic strains should be able to survive passage through the human gastrointestinal tract. Human gastrointestinal tract survival of probiotics in a low-fat spread matrix has, however, never been tested. The objective of this randomized, double-blind, placebo-controlled human intervention study was to test the human gastrointestinal tract survival of Lactobacillus reuteri DSM 17938 and Lactobacillus rhamnosus GG after daily consumption of a low-fat probiotic spread by using traditional culturing, as well as molecular methods. Forty-two healthy human volunteers were randomly assigned to one of three treatment groups provided with 20 g of placebo spread (n = 13), 20 g of spread with a target dose of 1 x 10(9) CFU of L. reuteri DSM 17938 (n = 13), or 20 g of spread with a target dose of 5 x 10(9) CFU of L. rhamnosus GG (n = 16) daily for 3 weeks. Fecal samples were obtained before and after the intervention period. A significant increase, compared to the baseline, in the recovery of viable probiotic lactobacilli in fecal samples was demonstrated after 3 weeks of daily consumption of the spread containing either L. reuteri DSM 17938 or L. rhamnosus GG by selective enumeration. In the placebo group, no increase was detected. The results of selective enumeration were supported by quantitative PCR, detecting a significant increase in DNA resulting from the probiotics after intervention. Overall, our results indicate for the first time that low-fat spread is a suitable carrier for these probiotic strains.
Dommels
Yvonne E M
YE
Unilever R&D Vlaardingen, 3130 AC Vlaardingen, The Netherlands. yvonne.dommels@unilever.com
Kemperman
Robèr A
RA
Zebregs
Yvonne E M P
YE
Draaisma
René B
RB
Jol
Arne
A
Wolvers
Danielle A W
DA
Vaughan
Elaine E
EE
Albers
Ruud
R
D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D015169:Colony Count, Microbial; D004311:Double-Blind Method; D005243:Feces / Q000382:microbiology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006805:Human Experimentation; D006801:Humans; D052200:Limosilactobacillus reuteri / Q000502:physiology*; D052201:Lacticaseibacillus rhamnosus / Q000502:physiology*; D008297:Male; D050296:Microbial Viability; D008875:Middle Aged; D010919:Placebos / Q000008:administration & dosage; D019936:Probiotics / Q000008:administration & dosage* / Q000494:pharmacology*; D055815:Young Adult
D010919:Placebos
Am J Clin Nutr. 2006 Jun;83(6):1256-64; quiz 1446-7
16762934
J Nutr. 1994 Jan;124(1):18-23
8283290
Lett Appl Microbiol. 1997 May;24(5):361-4
9172443
Int J Food Sci Nutr. 2003 Jul;54(4):281-9
12850889
Int J Food Microbiol. 1995 Apr;25(2):199-203
7547151
Int J Food Microbiol. 1998 May 26;41(2):85-101
9704859
Antonie Van Leeuwenhoek. 2002 Aug;82(1-4):29-58
12369195
Syst Appl Microbiol. 2001 Jul;24(2):227-31
11518325
Ann Nutr Metab. 1992;36(3):162-6
1530285
Eur J Nutr. 2004 Jun;43 Suppl 2:II118-II173
15221356
Int J Food Microbiol. 2004 Aug 15;95(1):103-6
15240080
J Appl Microbiol. 2009 Feb;106(2):506-14
19200317
J Appl Microbiol. 2005;98(6):1410-7
15916653
Food Chem Toxicol. 1998 Dec;36(12):1085-94
9862651
Eur J Clin Nutr. 2006 Nov;60(11):1284-93
16721394
Appl Environ Microbiol. 2004 Feb;70(2):1176-81
14766603
Appl Environ Microbiol. 1999 Nov;65(11):4949-56
10543808
Nutr Rev. 2003 Mar;61(3):91-9
12723641
Ann Med. 1990 Feb;22(1):57-9
2184848
Gastroenterology. 2009 Jan;136(1):65-80
19026645
Appl Environ Microbiol. 2008 Oct;74(19):6032-40
18689509
Dig Dis Sci. 1992 Jan;37(1):121-8
1728516
Oral Microbiol Immunol. 2006 Apr;21(2):129-31
16476023
FEMS Microbiol Lett. 2005 Dec 1;253(1):75-82
16242863
J Appl Microbiol. 1998 Jul;85(1):88-94
9721659
Int J Food Microbiol. 2003 Jul 25;84(2):189-96
12781941
Appl Environ Microbiol. 1999 Jan;65(1):351-4
9872808
FEMS Microbiol Lett. 2000 Jun 15;187(2):167-73
10856652
J Microbiol Methods. 1999 Jun;36(3):181-92
10379804