Transcriptional memory and switching in the Plasmodium falciparumrif gene family.
The human malaria parasite Plasmodium falciparum expresses erythrocyte-surface directed variant antigens which are important virulence factors. Many are transcribed from multigene families and presumably their mode of expression is strictly controlled to guarantee immune evasion in the human host. In order to elucidate the dynamics of rif transcription and to investigate if rif switching is comparable to var switching we monitored rif variant gene expression in parasites with different cytoadhesive properties as well as after a number of reinvasions. We found identical transcripts in parasite lines with different adhesive phenotypes suggesting that rif genes do not have a critical role in determining the cytoadhesion specificity of infected erythrocytes. We show for the first time that rif genes may show a conserved mode of transcription, maintaining the previously dominant rif transcript in subsequent reinvasions, but also observed rapid switching at rates up to 45% per generation, much higher than for the var gene family.
Cabral
Fernanda Janku
FJ
Department of Parasitology, Institute for Biomedical Sciences, Av. Prof. Lineu Prestes, 1374, Room 7, University of São Paulo, São Paulo, ZIP 05508-900, Brazil.
Wunderlich
Gerhard
G
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis*; D002448:Cell Adhesion; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis; D010963:Plasmodium falciparum / Q000502:physiology*; D015800:Protozoan Proteins / Q000096:biosynthesis*
D000953:Antigens, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins