title: Investigation of the multixenobiotic resistance mechanism in the freshwater fishes western mosquitofish, Gambusia affinis, and bluegill sunfish, Lepomis macrochirus.
abstract: The purpose of the study was to evaluate multixenobiotic resistance mechanism expression as a biomarker for contaminant exposure in freshwater fishes. Exposure to a known mammalian inhibitor (verapamil, 10 1M) and inducer (rhodamine 123, 3 1M) on the transport protein P-glycoprotein (Pgp) was investigated in the liver of the western mosquitofish and bluegill sunfish. No differences in the activity or expression of Pgp were measured in either species using a fluorometric accumulation assay and western blot analyses. The preliminary results from this study indicate that this detoxification mechanism may not be a sensitive indicator of contaminant exposure in certain teleost species.
authors.affiliation: College of Pharmacy, The University of Louisiana at Monroe, Monroe, LA 71209-0470, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Damaré
authors.forename: A K
authors.initials: AK
authors.lastname: Kaddoumi
authors.forename: K N
authors.initials: KN
authors.lastname: Baer
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D005399:Fishes; D005618:Fresh Water; D020112:Rhodamine 123 / Q000494:pharmacology; D013045:Species Specificity; D014700:Verapamil / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015415:Biomarkers; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; D020112:Rhodamine 123; D014700:Verapamil