title: [Influence of electroacupuncture on hepatic cytochrome P450 1 A 1 expression and lipid peroxidation in nonalcoholic fatty liver rats].
abstract: OBJECTIVE
  To study the effect of electroacupuncture (EA) on liver cytochrome P450 1 A 1 immunoactivity, superoxide dismutase (SOD) and malondialdehyde (MDA) contents in nonalcoholic fatty liver disease (NAFLD) rats.
  
  
  METHODS
  Thirty male SD rats were randomized into normal control (n = 10), model (n = 10) and EA (n = 10) groups. NAFLD model was established by feeding the animal with high-fat forage for 8 weeks. EA (1.6-2 Hz, 1-4 mA) was applied to bilateral "Zusanli" (ST 36), "Fenglong" (ST 40), "Sanyinjiao" (SP 6) and "Taichong" (LR 3) for 15 min, once daily for 4 weeks. Then the rats anesthetized with ether were killed for collecting liver tissue. Following homogenate and centrifugalization of the partial liver tissue, the supernatant was collected for assaying superoxide dismutase (SOD) and malondialdehyde (MDA) contents by xanthinoxidase chromometry and thio-malonylurea chromometry respectively. The other partial liver tissue samples were fixed in 10% formalin, followed by paraffin imbedding and sectioning (4 microm), and staining with streptavidin-perosidase methods respectively for displaying hepatic pathological changes and cytochrom P450 1 A 1 immunoreaction.
  
  
  RESULTS
  Compared with control group, hepatic SOD content of model group was significantly lower (P < 0.05), and MDA level and cytochrome P450 1 A 1 (CYP 1 A 1) integrated optic density (IOD) value were obviously higher in model group (P < 0.05). In comparison with model group, liver SOD level of EA group increased considerably (P < 0.05), while MDA level and CYP 1 A 1 IOD of EA group decreased evidently in EA group (P < 0.05).
  
  
  CONCLUSIONS
  EA can effectively reduce lipid peroxidation and up-regulate CYP 1 A 1 expression in nonalcoholic fatty liver tissue, which may contribute to its effect in improving fatty liver.
authors.affiliation: School of Chinese Medicine, Chongqing Medical University, Chongqing 400050, China. moonly1981@163.com
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: Feng
authors.forename: Qun-Ying
authors.initials: QY
authors.lastname: Liu
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Zeng
authors.forename: Lian-Shi
authors.initials: LS
authors.lastname: Zhou
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis*; D015671:Electroacupuncture; D005234:Fatty Liver / Q000378:metabolism / Q000628:therapy*; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase