title: New approach for m-cell-specific molecules screening by comprehensive transcriptome analysis.
abstract: A minor population of M cells within the follicle-associated epithelium (FAE) of intestinal Peyer's patches (PPs) serves as a major portal for entry of exogenous antigens. Characterization of the mammalian M cells, including identification of M-cell surface molecules used for bacterial uptake, has been hampered by their relative rarity. In contrast, M cells constitute virtually all of the FAE cells in the avian bursa of Fabricius. We therefore performed comparative gene expression profiling of chicken and murine FAE to identify commonly expressed genes by M cells in both species. The comprehensive transcriptome analysis revealed that 28 genes were commonly up-regulated in FAE from both species. In situ hybridization revealed that annexin A10 (Anxa10) mRNA was scattered in FAE, and co-localized with Ulex europaeus agglutinin-1 binding to M cells. Whole-mount immunostaining also revealed that cellular prion protein (PrP(C)) was expressed on the luminal side of the apical plasma membrane of M cells, and co-localized with grycoprotein 2 that recognizes only M cells in murine PP. Our findings identify new M-cell-specific molecules through using comprehensive transcriptome analysis. These conserved molecules in M cells of mice and chickens may play essential roles in M-cell function and/or differentiation.
authors.affiliation: International Graduate School of Arts and Sciences, Yokohama City University, Kanagawa 230-0045, Japan.
authors.forename: Gaku
authors.initials: G
authors.lastname: Nakato
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
authors.forename: Koji
authors.initials: K
authors.lastname: Hase
authors.forename: Ryo
authors.initials: R
authors.lastname: Goitsuka
authors.forename: Max D
authors.initials: MD
authors.lastname: Cooper
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohno
mesh_terms: D000818:Animals; D017302:Annexins / Q000378:metabolism*; D002060:Bursa of Fabricius / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D010581:Peyer's Patches / Q000166:cytology* / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism; D018621:PrPC Proteins / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D015854:Up-Regulation
chemical_list: D017302:Annexins; C485665:Anxa10 protein, mouse; D006023:Glycoproteins; D037121:Plant Lectins; D018621:PrPC Proteins; D011506:Proteins; C036850:Ulex europaeus lectins
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