title: PMA induces expression from the herpes simplex virus thymidine kinase promoter via the activation of JNK and ERK in the presence of adenoviral E1A proteins.
abstract: The herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) promoter contains elements involved in both constitutive and induced expression. We determined that phorbol 12-myristate 13-acetate (PMA) induces the HSV-1 TK promoter in HEK293 cells. However, PMA did not induce expression from the promoter in HeLa cells and did not result in a globally increased gene expression in HEK293 cells. Induction of HSV-1 TK promoter required activation of both of JNK and ERK pathways. However, activation of the two pathways alone was not sufficient for induction of HSV-1 TK promoter. By transiently transfecting into HeLa cells the adenoviral E1A gene, which exists as an integrant in HEK293 genome, we demonstrated that E1A proteins are necessary for induction of HSV-1 TK promoter by PMA. We propose mechanisms by which signaling pathways activated by the tumor-promoter PMA cooperate with the oncogene E1A to stimulate a eukaryotic promoter, namely the HSV-1 TK promoter.
authors.affiliation: Department of Internal Medicine, Albert Einstein College of Medicine, Bronx, NY, USA. shiferaa@vision.ucsf.edu
authors.forename: Amde Selassie
authors.initials: AS
authors.lastname: Shifera
authors.forename: John A
authors.initials: JA
authors.lastname: Hardin
mesh_terms: D017500:Adenovirus E1A Proteins / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D017930:Genes, Reporter; D005814:Genes, Viral; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D049410:Luciferases, Renilla / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013937:Thymidine Kinase / Q000235:genetics*
chemical_list: D017500:Adenovirus E1A Proteins; D017931:DNA Primers; D012333:RNA, Messenger; D049410:Luciferases, Renilla; D013937:Thymidine Kinase; D048031:JNK Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D013755:Tetradecanoylphorbol Acetate
references.citation: Nucleic Acids Res. 1987 Nov 11;15(21):9043-55
references.pmid: 3684579
references.citation: Cancer Res. 1968 Nov;28(11):2338-49
references.pmid: 5723975
references.citation: Virology. 1997 Jul 7;233(2):423-9
references.pmid: 9217065
references.citation: J Biol Chem. 1998 Jul 17;273(29):18623-32
references.pmid: 9660836
references.citation: Cancer Res. 1965 Dec;25(11):1871-5
references.pmid: 5856516
references.citation: J Virol. 1997 May;71(5):3545-53
references.pmid: 9094626
references.citation: Biochem Pharmacol. 1984 Mar 15;33(6):933-40
references.pmid: 6324806
references.citation: Proc Natl Acad Sci U S A. 1985 Aug;82(16):5265-9
references.pmid: 2991915
references.citation: Cell. 1981 Aug;25(2):385-98
references.pmid: 6269744
references.citation: J Virol. 1996 Dec;70(12):8459-67
references.pmid: 8970968
references.citation: Cancer Res. 1987 Feb 1;47(3):803-8
references.pmid: 2948635
references.citation: Proc Natl Acad Sci U S A. 1981 Oct;78(10):6276-80
references.pmid: 6273861
references.citation: Mol Cell Biol. 1987 Apr;7(4):1358-63
references.pmid: 3600629
references.citation: Nature. 1994 Apr 7;368(6471):520-5
references.pmid: 8139685
references.citation: Cell. 1985 Sep;42(2):559-72
references.pmid: 2992804
references.citation: Nucleic Acids Res. 1992 Feb 25;20(4):920
references.pmid: 1311835
references.citation: Bioessays. 1995 Jul;17(7):621-9
references.pmid: 7646484
references.citation: Cancer Res. 1988 Jan 1;48(1):1-8
references.pmid: 3275491
references.citation: Curr Top Microbiol Immunol. 1995;199 ( Pt 3):59-80
references.pmid: 7555084
references.citation: Mol Cells. 1999 Jun 30;9(3):277-80
references.pmid: 10420986
references.citation: J Biotechnol. 2001 Jul 12;88(3):251-8
references.pmid: 11434970
references.citation: Science. 1986 Oct 3;234(4772):53-9
references.pmid: 3018926
references.citation: J Biol Chem. 1996 May 24;271(21):12261-8
references.pmid: 8647824
references.citation: Curr Top Microbiol Immunol. 1995;199 ( Pt 3):25-32
references.pmid: 7555080
references.citation: J Cell Biochem. 2008 Apr 15;103(6):1732-46
references.pmid: 17902169
references.citation: Am J Physiol Lung Cell Mol Physiol. 2002 Sep;283(3):L619-27
references.pmid: 12169582
references.citation: Mol Cell Biol. 1984 Mar;4(3):563-6
references.pmid: 6717433
references.citation: Nature. 1984 Oct 4-10;311(5985):433-8
references.pmid: 6090941
references.citation: Nature. 1986 Oct 9-15;323(6088):555-8
references.pmid: 3020435
references.citation: Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13681-6
references.pmid: 11717429
references.citation: J Cell Sci. 2004 Dec 1;117(Pt 25):5965-73
references.pmid: 15564374
references.citation: Curr Top Microbiol Immunol. 2003;272:97-129
references.pmid: 12747548
references.citation: Mol Cell Biochem. 2008 Mar;310(1-2):181-90
references.pmid: 18080803
references.citation: J Biol Chem. 1991 Aug 25;266(24):15771-81
references.pmid: 1874734
references.citation: J Virol. 2004 Jun;78(12):6162-70
references.pmid: 15163709
references.citation: Am J Respir Cell Mol Biol. 1994 Apr;10(4):448-52
references.pmid: 8136160
references.citation: BMC Biotechnol. 2004 Apr 30;4:10
references.pmid: 15119957
references.citation: Biochem J. 2003 Dec 15;376(Pt 3):545-52
references.pmid: 14570590
references.citation: Nucleic Acids Res. 1989 Dec 25;17(24):10179-90
references.pmid: 2557588
references.citation: Biochem Biophys Res Commun. 1993 Mar 15;191(2):662-8
references.pmid: 8384849
references.citation: Curr Opin Cell Biol. 1996 Apr;8(2):168-73
references.pmid: 8791416
references.citation: FASEB J. 1999 Oct;13(13):1658-76
references.pmid: 10506570
references.citation: Biochem Soc Trans. 1997 Feb;25(1):303-8
references.pmid: 9056888
references.citation: Mol Cell Biol. 1990 Dec;10(12):6709-17
references.pmid: 2247079
references.citation: Curr Top Microbiol Immunol. 1995;199 ( Pt 3):1-23
references.pmid: 7555072
references.citation: DNA Cell Biol. 2001 Sep;20(9):563-8
references.pmid: 11747607
references.citation: Intervirology. 1991;32(2):76-92
references.pmid: 1851146
references.citation: J Biol Chem. 1994 Jul 8;269(27):17893-8
references.pmid: 8027044
references.citation: J Biol Chem. 1991 Mar 25;266(9):5670-5
references.pmid: 1900839
references.citation: J Biol Chem. 1982 Jul 10;257(13):7847-51
references.pmid: 7085651
references.citation: Biotechniques. 2001 Sep;31(3):528-30, 532
references.pmid: 11570496
references.citation: Biotechniques. 2000 Oct;29(4):782-4
references.pmid: 11056808
references.citation: Science. 1982 Jul 23;217(4557):316-24
references.pmid: 6283634
references.citation: Biochem J. 2000 Oct 1;351(Pt 1):95-105
references.pmid: 10998351
references.citation: J Cell Sci. 2004 Jan 15;117(Pt 2):131-2
references.pmid: 14676268
references.citation: Virus Res. 1990 Jan;15(1):57-68
references.pmid: 2156390
references.citation: Mol Cell Biol. 1995 May;15(5):2809-18
references.pmid: 7739562
references.citation: J Biol Chem. 1990 Jun 5;265(16):9402-12
references.pmid: 2160977
references.citation: J Steroid Biochem Mol Biol. 1999 Sep-Oct;70(4-6):197-201
references.pmid: 10622408
references.citation: Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:829-38
references.pmid: 6942952
references.citation: In Vitro Cell Dev Biol Anim. 2001 Jul-Aug;37(7):465-70
references.pmid: 11573823
references.citation: Proc Natl Acad Sci U S A. 1984 May;81(10):2975-9
references.pmid: 6328483
references.citation: Mol Cell Biol. 1992 Jun;12(6):2599-605
references.pmid: 1534142
references.citation: Nat Rev Mol Cell Biol. 2002 Jun;3(6):441-52
references.pmid: 12042766
references.citation: Am J Physiol. 1996 Apr;270(4 Pt 1):L535-40
references.pmid: 8928812
references.citation: Biochem Biophys Res Commun. 2004 Jan 9;313(2):300-7
references.pmid: 14684160
references.citation: J Virol. 1997 Dec;71(12):9600-7
references.pmid: 9371624
references.citation: EMBO J. 1993 Sep;12(9):3559-72
references.pmid: 8253081
references.citation: Prostate. 2004 May 1;59(2):115-9
references.pmid: 15042611
references.citation: Nucleic Acids Res. 1992 Feb 25;20(4):897-902
references.pmid: 1542579