title: Low-level laser therapy using 80-Hz pulsed infrared diode laser accelerates third-degree burn healing in rat.
abstract: OBJECTIVE
  To evaluate 80-Hz pulsed infrared diode low-level laser therapy (LLLT) for third-degree burn healing in rats.
  
  
  BACKGROUND
  Review of the literature indicates that LLLT has a biostimulatory effect on wound healing, although no clear recommendation can be made yet.
  
  
  METHODS
  Forty-eight male rats were divided into groups 1 and 2. On day 0, three third-degree burns were made on the dorsum of each rat using steam. In group 1, the first burn was exposed to an 80-Hz pulsed 890-nm infrared diode laser at 0.396 J/cm2 three times a week. In group 2, the first burn was exposed to the inactivated laser. In both groups, the second burns were treated with topical application of nitrofurazone 0.2%, and third burns did not receive any treatment and were considered as control burns. Burns were clinically examined. The rats were treated for 8, 12, and 20 days in both groups. On days 8, 2, and 20, microbiological samples of burns were collected and analyzed. Data were analyzed using the chi-square method.
  
  
  RESULTS
  The paired Student t-test showed that the wound closure rate of laser-treated burns (17.6+/-1.86 days) was significantly longer than that of the control burns (19.6+/-0.81 days) (p=0.007).The number of laser-treated burns that closed was significantly higher than controls. Chi-square tests showed that there was not significant difference between each microorganism (Staphylococcus epidermis, S. aureus and Pseudomonas aeruginosa).
  
  
  CONCLUSIONS
  LLLT using an 80-Hz pulsed infrared diode laser accelerated third-degree burn healing in rat.
authors.affiliation: Basic Sciences Department, Paramedical Faculty, Shahid Beheshti University, M.C., and Pathology Department, 501 Army Hospital, Tehran, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Vasheghani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Dadpay
authors.forename: Malihe
authors.initials: M
authors.lastname: Habibie
authors.forename: Fatemehsadat
authors.initials: F
authors.lastname: Rezaei
mesh_terms: D000818:Animals; D002056:Burns / Q000382:microbiology / Q000532:radiotherapy*; D016009:Chi-Square Distribution; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000528:radiation effects*