Cloning and characterization of peroxiredoxin in Babesia bovis.
We have identified the 2-Cys peroxiredoxin (Prx) from a bovine Babesia parasite, B. bovis. Prx is a recently described family of antioxidant enzymes that are highly conserved in eukaryotes and prokaryotes. B. bovis 2-Cys Prx (BbTPx-1) contained two conserved cysteine residues that corresponded to Cys47 and Cys170 of the yeast Prx and the amino acid sequences of two catalytic domains showed significant similarities to those of mammalian typical 2-Cys Prx. The antioxidant activity of the recombinant BbTPx-1 protein expressed in E. coli was demonstrated by a thiol mixed-function oxidation assay. Furthermore, we confirmed that BbTPx-1 was expressed in the cytoplasm of intra-erythrocytic B. bovis merozites. These results suggest that B. bovis likely uses TPx-1 as a way to reduce peroxides as a control of its intracellular redox balance so that it can live and grow in the host cell.
Tanaka
Miho
M
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Sakurai
Tatsuya
T
Yokoyama
Naoaki
N
Inoue
Noboru
N
Kawazu
Shin-ichiro
S
D000595:Amino Acid Sequence; D000818:Animals; D016793:Babesia bovis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000662:veterinary; D020134:Catalytic Domain; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003593:Cytoplasm / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment
D054464:Peroxiredoxins
J Biol Chem. 1994 Nov 4;269(44):27670-8
7961686
Mol Microbiol. 2004 Sep;53(5):1291-305
15387810
Mol Biochem Parasitol. 1992 Oct;55(1-2):85-94
1279421
Parasitology. 2005 May;130(Pt 5):489-92
15991491
Parasitol Today. 1999 Sep;15(9):357-60
10461161
Int J Parasitol. 2004 Feb;34(2):163-89
15037104
Antioxid Redox Signal. 2006 Jul-Aug;8(7-8):1227-39
16910770
Parasitol Int. 2008 Mar;57(1):1-7
17890140
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7017-21
8041738
Parasitol Today. 1998 Apr;14(4):139-45
17040731
Parasitology. 1992 Oct;105 ( Pt 2):177-82
1454416
Methods Enzymol. 1999;300:219-26
9919524
Parasitol Res. 1998;84(1):75-7
9491431
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
J Vet Med Sci. 1997 Jun;59(6):479-81
9234227
Angew Chem Int Ed Engl. 2005 Jan 21;44(5):690-715
15657967
Biochem Biophys Res Commun. 1995 Mar 28;208(3):964-9
7702627