title: Dynamics of T cell, antigen-presenting cell, and pathogen interactions during recall responses in the lymph node.
abstract: Memory T cells circulate through lymph nodes where they are poised to respond rapidly upon re-exposure to a pathogen; however, the dynamics of memory T cell, antigen-presenting cell, and pathogen interactions during recall responses are largely unknown. We used a mouse model of infection with the intracellular protozoan parasite, Toxoplasma gondii, in conjunction with two-photon microscopy, to address this question. After challenge, memory T cells migrated more rapidly than naive T cells, relocalized toward the subcapsular sinus (SCS) near invaded macrophages, and engaged in prolonged interactions with infected cells. Parasite invasion of T cells occurred by direct transfer of the parasite from the target cell into the T cell and corresponded to an antigen-specific increase in the rate of T cell invasion. Our results provide insight into cellular interactions during recall responses and suggest a mechanism of pathogen subversion of the immune response.
authors.affiliation: Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Chtanova
authors.forename: Seong-Ji
authors.initials: SJ
authors.lastname: Han
authors.forename: Marie
authors.initials: M
authors.lastname: Schaeffer
authors.forename: Giel G
authors.initials: GG
authors.lastname: van Dooren
authors.forename: Paul
authors.initials: P
authors.lastname: Herzmark
authors.forename: Boris
authors.initials: B
authors.lastname: Striepen
authors.forename: Ellen A
authors.initials: EA
authors.lastname: Robey
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000469:parasitology; D039521:CD11c Antigen / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000276:immunology*; D007156:Immunologic Memory; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000276:immunology
chemical_list: D039521:CD11c Antigen; D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse
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