Intraspecific hybrids of Arabidopsis thaliana revealed no gross alterations in endopolyploidy, DNA methylation, histone modifications and transcript levels.
Arabidopsis accessions Col-0 and C24 and their reciprocal hybrids were employed as a model system to investigate the potential relationship between changes in DNA methylation, chromatin structure, endopolyploidization and gene expression in heterotic genotypes. Nucleolus size, endopolyploidization level and distribution of DNA and histone H3 methylation at the microscopic level does not differ between parents and their hybrids. Methylation sensitive amplified polymorphism revealed a largely constant pattern of DNA methylation (97% of signals analyzed) after intraspecific crosses. The parental expression profile of selected genes was maintained in hybrid offspring. No correlation was found between expression pattern and DNA methylation levels at restriction sites within 5' regulatory regions. Thus, the results revealed only minor changes of chromatin properties and other nuclear features in response to intraspecific hybridization in Arabidopsis thaliana.
Banaei Moghaddam
Ali Mohammad
AM
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr 3, 06466 Gatersleben, Germany.
Fuchs
Jörg
J
Czauderna
Tobias
T
Houben
Andreas
A
Mette
Michael Florian
MF
D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002466:Cell Nucleolus / Q000648:ultrastructure; D019175:DNA Methylation; D006657:Histones / Q000378:metabolism*; D006823:Hybrid Vigor; D006824:Hybridization, Genetic; D007178:Inbreeding; D008745:Methylation; D011123:Polyploidy; D012333:RNA, Messenger / Q000378:metabolism*
D029681:Arabidopsis Proteins; D006657:Histones; D012333:RNA, Messenger
J Hered. 1977 Nov-Dec;68(6):413-5
608948
Genetics. 2007 Oct;177(2):749-60
17660570
Plant Cell. 2004 Feb;16(2):510-22
14729913
Plant Physiol. 2002 Jun;129(2):733-46
12068115
Nat Rev Genet. 2005 Nov;6(11):836-46
16304599
Plant J. 2003 Mar;33(6):967-73
12631322
Theor Appl Genet. 2006 Sep;113(5):831-45
16868764
Mol Genet Genomics. 2002 Dec;268(4):543-52
12471452
Methods. 2001 Dec;25(4):402-8
11846609
Mol Cell. 2000 Oct;6(4):791-802
11090618
Mol Genet Genomics. 2003 Nov;270(3):281-6
14551762
Cell. 2007 Feb 23;128(4):693-705
17320507
Planta. 2005 Oct;222(2):301-6
15968513
Nat Rev Mol Cell Biol. 2003 Aug;4(8):641-9
12923526
Genome. 2000 Aug;43(4):679-88
10984181
Chromosoma. 2004 Nov;113(5):258-69
15480725
Nat Methods. 2005 Mar;2(3):213-8
16163802
Plant Physiol. 1991 Jul;96(3):985-9
16668285
Mol Biol Evol. 2002 Aug;19(8):1218-27
12140233
Stain Technol. 1980 Mar;55(2):87-90
6157230
Mol Gen Genet. 1999 Apr;261(3):439-46
10323223
Plant Cell. 2003 Oct;15(10):2236-9
14523245
Genetics. 2002 Sep;162(1):355-63
12242246
Trends Plant Sci. 2007 Sep;12(9):427-32
17720610
Mol Ecol. 2005 Apr;14(4):1163-75
15773943
Genetics. 2006 Jan;172(1):507-17
16172500
Genetics. 2008 Jul;179(3):1275-83
18562640
Genome. 2008 Oct;51(10):789-99
18923530
Proc Natl Acad Sci U S A. 2006 May 2;103(18):6805-10
16641103
Mol Cell. 2004 Feb 27;13(4):599-609
14992728
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6753-8
11371624
Genome. 2001 Jun;44(3):321-30
11444689
PLoS Biol. 2007 Jul;5(7):e174
17579518
Genetics. 1987 Oct;117(2):285-95
17246404
Cytogenet Genome Res. 2005;109(1-3):236-49
15753583
Science. 2008 Apr 25;320(5875):489-92
18436779
PLoS Genet. 2008 Apr 04;4(4):e1000048
18389059
Mol Biol (Mosk). 2008 Mar-Apr;42(2):195-205
18610827
Planta. 2009 Feb;229(3):471-83
18998158
Growth. 1979 Dec;43(4):252-62
546689
Cell. 2006 Sep 22;126(6):1189-201
16949657
Mech Ageing Dev. 1981 Oct;17(2):141-50
6796784
Plant Mol Biol. 2007 Feb;63(3):381-91
17082872
Biochem Genet. 1976 Apr;14(3-4):281-92
962844
Plant Mol Biol. 2000 Jun;43(2-3):387-99
10999418
Stain Technol. 1985 Sep;60(5):285-94
2412317
Plant Mol Biol. 2007 Jan;63(1):21-34
17006594
Plant Physiol. 2004 Apr;134(4):1813-23
15064384
Trends Plant Sci. 2006 Apr;11(4):199-208
16546438
Curr Opin Plant Biol. 2003 Dec;6(6):544-53
14611952
Trends Genet. 2004 Jun;20(6):244-51
15145577