The in vitro elution characteristics of vancomycin from calcium phosphate-calcium sulfate beads.
The purpose of this study was to determine the elution characteristics of vancomycin when combined with a calcium phosphate-calcium sulfate mixture to form antibiotic beads in an in vitro environment. The beads were placed in phosphate-buffered saline and kept at 36 degrees C for 6 weeks. Three separate assays were undertaken, after which the phosphate-buffered saline was assayed at 24-hour intervals. Elution of the antibiotic was sustained at a gradual rate for 22 days. The amount of vancomycin assayed nearly equaled the calculated amount of antibiotic per bead measured during bead construction. In conclusion, calcium phosphate-calcium sulfate composite beads, when combined with vancomycin, elute vancomycin in a gradual and sustained fashion for 22 days in vitro.


METHODS
2.
Scharer
Brandon M
BM
Wheaton Franciscan Healthcare, Milwaukee, WI 53222, USA. Brandon.Scharer@dmu.edu
Sanicola
Shawn M
SM
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000737:chemistry* / Q000627:therapeutic use; D002133:Calcium Sulfate / Q000737:chemistry* / Q000627:therapeutic use; D003764:Dental Materials / Q000737:chemistry* / Q000627:therapeutic use; D016503:Drug Delivery Systems; D004359:Drug Therapy, Combination; D006801:Humans; D066298:In Vitro Techniques; D014640:Vancomycin / Q000737:chemistry* / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D003764:Dental Materials; D014640:Vancomycin; C020243:calcium phosphate; D002133:Calcium Sulfate