title: Epitope mapping immunodominant regions of the PilA protein of nontypeable Haemophilus influenzae (NTHI) to facilitate the design of two novel chimeric vaccine candidates.
abstract: We designed and tested three PilA-derived vaccine candidates in a chinchilla model of ascending nontypeable Haemophilus influenzae (NTHI)-induced otitis media (OM). Delivery of antiserum directed against each immunogen conferred varying degrees of protection. Presentation of a B-cell epitope derived from the OMP P5 adhesin at the N-terminus of recombinant soluble PilA protein (as opposed to the C-terminus), resulted in a protective chimeric immunogen that combined epitopes from two distinct NTHI adhesins (type IV pili and OMP P5). Incorporating protective epitopes derived from two NTHI adhesins/virulence determinants into a single pediatric vaccine candidate to prevent OM has multiple potential inherent advantages.
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Center for Microbial Pathogenesis and The Ohio State University College of Medicine, 700 Children's Drive, W591, Columbus, OH 43205, United States.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Novotny
authors.forename: Leanne D
authors.initials: LD
authors.lastname: Adams
authors.forename: D Richard
authors.initials: DR
authors.lastname: Kang
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Wiet
authors.forename: Xueya
authors.initials: X
authors.lastname: Cai
authors.forename: Sanjay
authors.initials: S
authors.lastname: Sethi
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Murphy
authors.forename: Lauren O
authors.initials: LO
authors.lastname: Bakaletz
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D015374:Biosensing Techniques; D002675:Child, Preschool; D002682:Chinchilla; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control*; D006193:Haemophilus influenzae / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D007223:Infant; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010034:Otitis Media with Effusion / Q000276:immunology / Q000517:prevention & control*; D017434:Protein Structure, Tertiary; D029424:Pulmonary Disease, Chronic Obstructive / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D018985:Epitopes, B-Lymphocyte; D016056:Immunodominant Epitopes
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