Immunofluorescent microscopic study of meiosis in zebrafish.
In recent years, zebrafish (Danio rerio) has been used as a model vertebrate organism for studies of human disease, development, and genetics. This chapter describes detailed methods for the preparation of whole-mount meiotic oocytes and spermatocytes as well as cryostat sectioning of ovaries and testes for immunofluorescence microscopy studies of zebrafish.
Kochakpour
Nazafarin
N
Department of Biology, York University, Toronto, Ontario, Canada.
D000818:Animals; D003584:Cytological Techniques / Q000379:methods; D008540:Meiosis / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000379:methods; D016707:Tissue Fixation / Q000379:methods; D015027:Zebrafish / Q000235:genetics*