[Effects of borneol on pharmacokinetics of protocatechuic acid in rabbits].
OBJECTIVE
To study the effect of borneol on the pharmacokinetics of protocatechuic acid (PA) in rabbits.


METHODS
A method for determinating PA in rabbit plasma by high performance liquid chromatography (HPLC) was established. The 12 healthy New Zealand rabbits were randomly divided into 2 groups. PA concentration in plasma samples of rabbits was determined by HPLC after oral administration of PA (30 mg x kg(-1)) and PA combined with borneol (protocatechuic acid 30 mg x kg(-1) + borneol 15 mg x kg(-1)). The data were processed by DAS 2.0 software to calculate the pharmacokinetic parametes.


RESULTS
Chromatographic peaks were separated well by the HPLC method. Regression analysis of the data of PA concentration against its peak area showed a good straight line in the range of 0.04-2.0 mg x L(-1) (r = 0.9983). In comparison with PA alone, the pharmacokinetics parameters of PA (t1/2Ka, T(max), V1/F, CL/F) in the PA + borneol combination group were decreased, while the AUC(0-infinity) was increased.


CONCLUSIONS
Borneol could significantly promote absorption, increase the dose absorption and slow elimination of PA in rabbits. Borneol could improve the bioavailability of PA.
Yang
Jie
J
The College of Life Sciences, Northwest University, Xi'an 710069, China.
Wang
Shixiang
S
Lan
Wei
W
Liu
Chunzhi
C
Zheng
Xiaohui
X
D000818:Animals; D001892:Camphanes / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D062385:Hydroxybenzoates / Q000097:blood / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D011817:Rabbits; D012044:Regression Analysis; D015203:Reproducibility of Results
D001892:Camphanes; D062385:Hydroxybenzoates; C009091:protocatechuic acid; C022871:isoborneol