Drosophila suppressor of sable protein [Su(s)] promotes degradation of aberrant and transposon-derived RNAs.
RNA-binding proteins act at various stages of gene expression to regulate and fine-tune patterns of mRNA accumulation. One protein in this class is Drosophila Su(s), a nuclear protein that has been previously shown to inhibit the accumulation of mutant transcripts by an unknown mechanism. Here, we have identified several additional RNAs that are downregulated by Su(s). These Su(s) targets include cryptic wild-type transcripts from the developmentally regulated Sgs4 and ng1 genes, noncoding RNAs derived from tandemly repeated alphabeta/alphagamma elements within an Hsp70 locus, and aberrant transcripts induced by Hsp70 promoter transgenes inserted at ectopic sites. We used the alphabeta RNAs to investigate the mechanism of Su(s) function and obtained evidence that these transcripts are degraded by the nuclear exosome and that Su(s) promotes this process. Furthermore, we showed that the RNA binding domains of Su(s) are important for this effect and mapped the sequences involved to a 267-nucleotide region of an alphabeta element. Taken together, these results suggest that Su(s) binds to certain nascent transcripts and stimulates their degradation by the nuclear exosome.
Kuan
Yung-Shu
YS
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA.
Brewer-Jensen
Paul
P
Bai
Wen-Li
WL
Hunter
Cedric
C
Wilson
Carrie B
CB
Bass
Sarah
S
Abernethy
John
J
Wing
James S
JS
Searles
Lillie L
LL
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000378:metabolism / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005967:Glue Proteins, Drosophila / Q000235:genetics / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism*
D029721:Drosophila Proteins; D005967:Glue Proteins, Drosophila; D018840:HSP70 Heat-Shock Proteins; D016601:RNA-Binding Proteins; D012471:Salivary Proteins and Peptides; C424382:Sgs4 protein, Drosophila; C081602:ng1 protein, Drosophila; C416345:su(sable) protein, Drosophila; D012313:RNA; D012319:RNA Polymerase II
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