title: Virulence genes and molecular typing of different groups of Escherichia coli O157 strains in cattle.
abstract: Characterization of an Escherichia coli O157 strain collection (n = 42) derived from healthy Hungarian cattle revealed the existence of diverse pathotypes. Enteropathogenic E. coli (EPEC; eae positive) appeared to be the most frequent pathotype (n = 22 strains), 11 O157 strains were typical enterohemorrhagic E. coli (EHEC; stx and eae positive), and 9 O157 strains were atypical, with none of the key stx and eae virulence genes detected. EHEC and EPEC O157 strains all carried eae-gamma, tir-gamma, tccP, and paa. Other virulence genes located on the pO157 virulence plasmid and different O islands (O island 43 [OI-43] and OI-122), as well as espJ and espM, also characterized the EPEC and EHEC O157 strains with similar frequencies. However, none of these virulence genes were detected by PCR in atypical O157 strains. Interestingly, five of nine atypical O157 strains produced cytolethal distending toxin V (CDT-V) and carried genes encoding long polar fimbriae. Macro-restriction fragment enzyme analysis (pulsed-field gel electrophoresis) revealed that these E. coli O157 strains belong to four main clusters. Multilocus sequence typing analysis revealed that five housekeeping genes were identical in EHEC and EPEC O157 strains but were different in the atypical O157 strains. These results suggest that the Hungarian bovine E. coli O157 strains represent at least two main clones: EHEC/EPEC O157:H7/NM (nonmotile) and atypical CDT-V-producing O157 strains with H antigens different from H7. The CDT-V-producing O157 strains represent a novel genogroup. The pathogenic potential of these strains remains to be elucidated.
authors.affiliation: Veterinary Medical Research Institute of the Hungarian Academy of Sciences, 1143 Budapest, Hungary. tothi@vmri.hu
authors.forename: István
authors.initials: I
authors.lastname: Tóth
authors.forename: Herbert
authors.initials: H
authors.lastname: Schmidt
authors.forename: Gábor
authors.initials: G
authors.lastname: Kardos
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Lancz
authors.forename: Kristina
authors.initials: K
authors.lastname: Creuzburg
authors.forename: Ivelina
authors.initials: I
authors.lastname: Damjanova
authors.forename: Judit
authors.initials: J
authors.lastname: Pászti
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.forename: Béla
authors.initials: B
authors.lastname: Nagy
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D005838:Genotype; D006814:Hungary; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D037521:Virulence Factors
references.citation: Cell Microbiol. 2008 Jul;10(7):1429-41
references.pmid: 18331467
references.citation: Biochem Soc Trans. 2003 Dec;31(Pt 6):1326-9
references.pmid: 14641055
references.citation: Infect Immun. 2003 Jun;71(6):3634-8
references.pmid: 12761152
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):17043-8
references.pmid: 12481030
references.citation: Vet Res. 2005 May-Jun;36(3):289-311
references.pmid: 15845227
references.citation: J Clin Microbiol. 2005 Apr;43(4):1552-63
references.pmid: 15814965
references.citation: Infect Immun. 1998 Feb;66(2):480-5
references.pmid: 9453599
references.citation: Clin Infect Dis. 2007 Jul 1;45(1):39-45
references.pmid: 17554698
references.citation: Int J Med Microbiol. 2005 Oct;295(6-7):405-18
references.pmid: 16238016
references.citation: Vet Res. 2008 May-Jun;39(3):38
references.pmid: 18316019
references.citation: Infect Immun. 2002 Dec;70(12):6761-9
references.pmid: 12438351
references.citation: Cell Microbiol. 2008 May;10(5):1104-15
references.pmid: 18201246
references.citation: Clin Infect Dis. 2008 Jul 15;47(2):208-17
references.pmid: 18564929
references.citation: J Clin Microbiol. 1995 May;33(5):1375-7
references.pmid: 7615759
references.citation: Clin Infect Dis. 2005 Sep 15;41(6):785-92
references.pmid: 16107974
references.citation: J Clin Microbiol. 1998 Feb;36(2):598-602
references.pmid: 9466788
references.citation: J Clin Microbiol. 2003 Nov;41(11):4930-40
references.pmid: 14605120
references.citation: Infect Immun. 2004 Mar;72(3):1812-6
references.pmid: 14977993
references.citation: Appl Environ Microbiol. 2007 May;73(10):3144-50
references.pmid: 17400784
references.citation: Nat Rev Microbiol. 2004 Feb;2(2):123-40
references.pmid: 15040260
references.citation: J Clin Microbiol. 1994 Oct;32(10):2460-3
references.pmid: 7814482
references.citation: DNA Res. 2001 Feb 28;8(1):11-22
references.pmid: 11258796
references.citation: J Clin Microbiol. 2001 Dec;39(12):4541-3
references.pmid: 11724879
references.citation: Nature. 2001 Jan 25;409(6819):529-33
references.pmid: 11206551
references.citation: J Clin Microbiol. 2006 May;44(5):1844-6
references.pmid: 16672418
references.citation: Clin Microbiol Rev. 1998 Jan;11(1):142-201
references.pmid: 9457432
references.citation: FEMS Microbiol Lett. 2006 Oct;263(1):32-40
references.pmid: 16958848
references.citation: Appl Environ Microbiol. 1996 Dec;62(12):4314-7
references.pmid: 8953703
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4535-6
references.pmid: 18353979
references.citation: Appl Environ Microbiol. 1999 Sep;65(9):3855-61
references.pmid: 10473386
references.citation: J Clin Microbiol. 2003 Sep;41(9):4285-91
references.pmid: 12958258
references.citation: J Clin Microbiol. 2005 Feb;43(2):546-50
references.pmid: 15695643
references.citation: J Bacteriol. 2002 Sep;184(17):4690-8
references.pmid: 12169592
references.citation: Cell Microbiol. 2009 Feb;11(2):217-29
references.pmid: 19016787
references.citation: Microbiology (Reading). 2000 May;146 ( Pt 5):1085-1090
references.pmid: 10832635
references.citation: J Clin Microbiol. 1997 May;35(5):1066-70
references.pmid: 9114382
references.citation: Appl Environ Microbiol. 2001 Sep;67(9):4335-7
references.pmid: 11526041
references.citation: Infect Immun. 2007 Feb;75(2):604-12
references.pmid: 17101643
references.citation: Infect Immun. 1998 Sep;66(9):4496-8
references.pmid: 9712806
references.citation: Appl Environ Microbiol. 2003 Dec;69(12):7242-7
references.pmid: 14660372
references.citation: Infect Immun. 2000 Jan;68(1):64-71
references.pmid: 10603369
references.citation: Epidemiol Infect. 1990 Dec;105(3):511-20
references.pmid: 2249715
references.citation: Acta Vet Hung. 2003;51(1):29-44
references.pmid: 12688124
references.citation: Int J Med Microbiol. 2002 Sep;292(3-4):207-14
references.pmid: 12398211
references.citation: Mol Phylogenet Evol. 1992 Sep;1(3):242-52
references.pmid: 1342941
references.citation: Bioinformatics. 1998;14(1):68-73
references.pmid: 9520503
references.citation: Emerg Infect Dis. 2004 Aug;10(8):1482-5
references.pmid: 15496255
references.citation: Vet Microbiol. 1996 Sep;52(1-2):153-64
references.pmid: 8914259
references.citation: Appl Environ Microbiol. 1996 Sep;62(9):3462-5
references.pmid: 8795238
references.citation: Infect Immun. 2003 Jun;71(6):3343-8
references.pmid: 12761117
references.citation: J Clin Microbiol. 2005 Nov;43(11):5715-20
references.pmid: 16272509
references.citation: J Med Microbiol. 2009 Aug;58(Pt 8):988-995
references.pmid: 19528152
references.citation: BMC Microbiol. 2008 Oct 01;8:166
references.pmid: 18828904
references.citation: Microbiol Immunol. 1993;37(7):543-8
references.pmid: 8231968
references.citation: J Clin Pathol. 1958 Jan;11(1):71-7
references.pmid: 13502467