Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells.
Human umbilical cord blood-derived mesenchymal stem cells (MSCs) are known to possess the potential for multiple differentiations abilities in vitro and in vivo. In canine system, studying stem cell therapy is important, but so far, stem cells from canine were not identified and characterized. In this study, we successfully isolated and characterized MSCs from the canine umbilical cord and its fetal blood. Canine MSCs (cMSCs) were grown in medium containing low glucose DMEM with 20% FBS. The cMSCs have stem cells expression patterns which are concerned with MSCs surface markers by fluorescence- activated cell sorter analysis. The cMSCs had multipotent abilities. In the neuronal differentiation study, the cMSCs expressed the neuronal markers glial fibrillary acidic protein (GFAP), neuronal class III beta tubulin (Tuj-1), neurofilament M (NF160) in the basal culture media. After neuronal differentiation, the cMSCs expressed the neuronal markers Nestin, GFAP, Tuj-1, microtubule-associated protein 2, NF160. In the osteogenic & chondrogenic differentiation studies, cMSCs were stained with alizarin red and toluidine blue staining, respectively. With osteogenic differentiation, the cMSCs presented osteoblastic differentiation genes by RT-PCR. This finding also suggests that cMSCs might have the ability to differentiate multipotentially. It was concluded that isolated MSCs from canine cord blood have multipotential differentiation abilities. Therefore, it is suggested that cMSCs may represent a be a good model system for stem cell biology and could be useful as a therapeutic modality for canine incurable or intractable diseases, including spinal cord injuries in future regenerative medicine studies.
Seo
Min-Soo
MS
Adult Stem Cell Research Center, Department of Veterinary Public Health, College of Veterinery Medicine, Seoul National University, Seoul 151-742, Korea.
Jeong
Yun-Hyeok
YH
Park
Jeung-Ran
JR
Park
Sang-Bum
SB
Rho
Kyoung-Hwan
KH
Kim
Hyung-Sik
HS
Yu
Kyung-Rok
KR
Lee
Seung-Hee
SH
Jung
Ji-Won
JW
Lee
Yong-Soon
YS
Kang
Kyung-Sun
KS
D000818:Animals; D002454:Cell Differentiation; D020219:Chondrogenesis; D004285:Dogs / Q000097:blood / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D010012:Osteogenesis
Stem Cells. 2006 May;24(5):1294-301
16410387
Bone. 2007 Feb;40(2):382-90
17049329
Stem Cells Dev. 2007 Aug;16(4):597-604
17784833
Blood. 2002 May 15;99(10):3838-43
11986244
Cytotherapy. 2004;6(6):543-53
15770794
Lab Anim Sci. 1990 Jan;40(1):11-5
2153851
Ann N Y Acad Sci. 1998 Jun 30;850:276-87
9668549
Spinal Cord. 2007 Jan;45(1):15-24
16773039
Stem Cells. 2005;23(1):3-9
15625118
Blood. 2004 Mar 1;103(5):1669-75
14576065
J Biomed Mater Res. 1999;48(6):913-27
10556859
Stem Cells. 2006 Apr;24(4):1054-64
16322639
Semin Hematol. 2007 Oct;44(4):259-66
17961725
Vet Immunol Immunopathol. 2004 Sep;101(1-2):31-47
15261691
Curr Protoc Immunol. 2005 Nov;Chapter 22:Unit 22A.1
18432946
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10614-9
9724752
J Neurosci Res. 2009 Feb;87(2):318-32
18803285
Am J Vet Res. 2005 Oct;66(10):1729-37
16273904
Cytotherapy. 2006;8(6):570-9
17148034
Trends Cell Biol. 2002 Nov;12(11):502-8
12446111
Haematologica. 2004 Jul;89(7):837-44
15257936
J Soc Biol. 2008;202(1):3-6
18460303
Biochem Biophys Res Commun. 2004 Aug 13;321(1):102-8
15358221
J Neurosci Res. 2006 Mar;83(4):627-37
16435396
Nature. 2002 Jul 4;418(6893):41-9
12077603
FASEB J. 2006 May;20(7):950-2
16585061
Stem Cell Rev. 2008 Spring;4(1):3-11
18286392
Blood. 2000 Jan 15;95(2):581-5
10627466
Biometals. 2006 Jun;19(3):315-22
16799869
Stem Cells. 2006 Aug;24(8):1869-78
16675597
J Allergy Clin Immunol. 2006 Apr;117(4):725-38; quiz 739
16630926
Exp Hematol. 2002 Jan;30(1):42-8
11823036
Science. 1999 Apr 2;284(5411):143-7
10102814
Stem Cells. 2004;22(4):625-34
15277708
Tissue Eng. 2005 May-Jun;11(5-6):787-802
15998219
Nat Cell Biol. 2004 Jun;6(6):532-9
15133469
Ann Hematol. 2004 Dec;83(12):733-8
15372203