Effect of nitric oxide donor, a modulator of tumor drug resistance, on cell death and p53 protein expression.
Exogenous NO donor 3,3-bis-(nitroxymethyl)oxetane (NMO) was synthesized at the Institute for Problems of Chemical Physics (Russian Academy of Sciences). This compound was shown to inhibit cell death (apoptosis and necrosis) in cyclophosphamide-sensitive and cyclophosphamide-resistant P388 murine tumor. p53 protein was expressed in both lines of tumor cells. NO donor NMO had little effect on p53 protein expression in cells of both stains. Our results suggest that the proapoptotic effect of NMO is mediated by the p53-independent molecular mechanisms.
Rajewskaya
T A
TA
Institute for Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia. tara@icp.ac.ru
Goncharova
S A
SA
Konovalova
N P
NP
Terent'ev
A A
AA
Lapshina
M A
MA
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002452:Cell Count; D045744:Cell Line, Tumor; D003520:Cyclophosphamide / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D004988:Ethers, Cyclic / Q000494:pharmacology*; D007941:Leukemia P388 / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D009336:Necrosis / Q000188:drug therapy / Q000503:physiopathology; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
C479364:3,3-bis(nitroxymethyl)oxetane; D018906:Antineoplastic Agents, Alkylating; D004988:Ethers, Cyclic; D020030:Nitric Oxide Donors; D016159:Tumor Suppressor Protein p53; D003520:Cyclophosphamide