Long-term immunogenicity and protection against Mycoplasma agalactiae induced by an oil adjuvant vaccine in sheep.
The long-term protective immunity of an inactivated mineral-oil adjuvanted Mycoplasma agalactiae vaccine was evaluated in sheep. The antigen suspension was emulsified with a mixture of three mineral oils (Montanide ISA-563, Marcol-52, Montane-80 at the ratio of 30%, 63%, and 7%, respectively). Twenty-two animals were divided in 2 groups (A and B) and immunised with two doses of the vaccine (group A, n=14) or used as unvaccinated control (group B, n=8). Five months after the second vaccination, seven animals of group A and four animals of group B were challenged by nasal route with M. agalactiae. The remaining seven vaccinated and four control animals were challenged intranasally eight months after vaccination. The vaccine was able to induce a full-protective immunity preventing the clinical signs of contagious agalactia and the infection by M. agalactiae in all groups of animals irrespective of the time of challenge after booster administration.
Buonavoglia
Domenico
D
Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy. d.buonavoglia@veterinaria.uniba.it
Greco
Grazia
G
Corrente
Marialaura
M
Greco
Maria Fiorella
MF
D'Abramo
Maria
M
Latronico
Francesca
F
Fasanella
Antonio
A
Decaro
Nicola
N
D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D008899:Mineral Oil; D009175:Mycoplasma Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D045644:Mycoplasma agalactiae / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012756:Sheep / Q000276:immunology / Q000382:microbiology; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D008899:Mineral Oil