Development of SSR markers for studies of diversity in the genus Fagopyrum.
The numbers of SSR markers and their utilization have not been determined and investigated as extensively in Fagopyrum species as compared to other crop species. The current report presents 136 new SSR markers in Fagopyrum esculentum ssp. esculentum and their application to related species in the genus Fagopyrum. Of the 136 SSRs, 10 polymorphic SSR markers were utilized in a genetic diversity analysis of a common buckwheat population consisting of 41 accessions of diverse origin. The study showed observed (H(O)) and expected (H(E)) heterozygosities ranging from 0.071 to 0.924 (mean = 0.53) and from 0.073 to 0.902 (mean = 0.412), respectively. Forty-one of the 136 SSRs amplified sequences in other Fagopyrum species, including the cymosum and urophyllum groups. The phylogenetic relationships revealed using the SSRs was consistent with results obtained using other marker systems, with one exception. The sequence and diversity information obtained using these new SSRs and their cross-transferability to related Fagopyrum species will increase our understanding of genetic structures and species relationships within the Fagopyrum genus.
Ma
Kyung-Ho
KH
National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, 88-20, Seodun-Dong, Suwon, Gyunggi-do 441-707, Korea.
Kim
Nam-Soo
NS
Lee
Gi-An
GA
Lee
Sok-Young
SY
Lee
Ju Kyong
JK
Yi
Jung Yoon
JY
Park
Yong-Jin
YJ
Kim
Tae-San
TS
Gwag
Jae-Gyun
JG
Kwon
Soon-Jae
SJ
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D019612:Fagopyrum / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D006579:Heterozygote; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers
Am J Bot. 1998 Aug;85(8):1134
21684998
Genes Genet Syst. 1998 Aug;73(4):201-10
9880918
Annu Rev Plant Biol. 2003;54:357-74
14502995
Am J Hum Genet. 1989 Mar;44(3):397-401
2563634
Theor Appl Genet. 2002 Aug;105(2-3):306-312
12582533
Theor Appl Genet. 2004 Apr;108(6):1147-50
15067402
Genes Genet Syst. 2005 Apr;80(2):113-9
16172523
Genes Genet Syst. 2002 Dec;77(6):399-408
12589075
Am J Hum Genet. 1989 Mar;44(3):388-96
2916582
Genetics. 2002 May;161(1):289-98
12019242
Mol Biol Evol. 2002 Aug;19(8):1251-60
12140237
Mol Biol Evol. 1998 Oct;15(10):1275-87
9787434
Nat Genet. 1996 Aug;13(4):390-1
8696328
Genes Genet Syst. 1996 Aug;71(4):219-24
8979374
Genes Genet Syst. 1996 Aug;71(4):211-8
8979373
Genome. 2005 Jun;48(3):367-77
16121234
Bioinformatics. 2012 Oct 1;28(19):2537-9
22820204
Bioinformatics. 2005 May 1;21(9):2128-9
15705655
Nat Biotechnol. 2000 Feb;18(2):233-4
10657137
Theor Appl Genet. 2004 Aug;109(4):783-91
15205737
Crit Rev Food Sci Nutr. 1983;19(3):213-58
6380955
Theor Appl Genet. 2006 May;112(8):1573-81
16596396
Trends Biotechnol. 2005 Jan;23(1):48-55
15629858
Nat Genet. 1995 Dec;11(4):360-2
7493011
Theor Appl Genet. 2005 Aug;111(4):723-30
15997389
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9161-6
11470895
Genome. 1993 Feb;36(1):181-6
18469981