Disposable microfluidic device with ultraviolet detection for highly resolved screening of illicit drugs.
A disposable microfluidic device was constructed by conveniently integrating one poly(methyl methacrylate) board with four reservoirs and one fractured fused-silica capillary with 50 microm i.d. and 7.5 cm total length on a printed circuit board for applying sampling and separation voltages. The disposable microfluidic device combined with a home-made ultraviolet workstation could be conveniently used for efficient screening and quantitative detection of microg mL(-1) illicit drugs. Using eight illicit drugs as models, they could be baseline-separated within 240 s with the separation efficiency up to 600,047 plates m(-1) at the designed device. The novel device and proposed protocol were successfully used to screen illicit drugs in human urine. This work presented a simple and low-cost method to fabricate the microfluidic device and provided a powerful way for sensitive and specific multi-screening of different drugs with high resolution, fast separation and low-cost.
Qiang
Wei
W
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing, 210093, China.
Zhai
Chun
C
Lei
Jianping
J
Song
Chaojin
C
Zhang
Daming
D
Sheng
Jin
J
Ju
Huangxian
H
D015104:3,4-Methylenedioxyamphetamine / Q000302:isolation & purification; D004209:Disposable Equipment; D013759:Dronabinol / Q000302:isolation & purification; D004809:Ephedrine / Q000302:isolation & purification; D004867:Equipment Design; D003932:Heroin / Q000302:isolation & purification; D006801:Humans; D013287:Illicit Drugs / Q000652:urine*; D007649:Ketamine / Q000302:isolation & purification; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009020:Morphine / Q000302:isolation & purification; D009022:Morphine Derivatives / Q000302:isolation & purification; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000295:instrumentation / Q000379:methods; D014466:Ultraviolet Rays
D013287:Illicit Drugs; D009022:Morphine Derivatives; D015104:3,4-Methylenedioxyamphetamine; D007649:Ketamine; D003932:Heroin; D009020:Morphine; D013759:Dronabinol; D004809:Ephedrine; C026979:6-O-monoacetylmorphine