title: The glutamate switch is present in all seven clades of AAA+ protein.
abstract: Recent work has identified a "glutamate switch" in six of the seven clades of AAA+ ATPases. The glutamate switch acts to transduce information regarding substrate binding to the ATPase active site. We provide biochemical evidence that a highly conserved threonine residue acts as a glutamate switch in the replicative helicase, MCM, and, thus, reveal that the glutamate switch is a feature common to all seven AAA+ clades.
authors.affiliation: Sir William Dunn School of Pathology, Oxford University, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Mogni
authors.forename: Alessandro
authors.initials: A
authors.lastname: Costa
authors.forename: Charikleia
authors.initials: C
authors.lastname: Ioannou
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Bell
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D020816:Amino Acid Motifs; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain / Q000235:genetics; D053488:DnaB Helicases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry* / Q000378:metabolism*; D026341:Minichromosome Maintenance 1 Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D040681:Structural Homology, Protein; D013379:Substrate Specificity / Q000235:genetics; D048229:Sulfolobus solfataricus / Q000201:enzymology; D013912:Threonine / Q000737:chemistry / Q000235:genetics
chemical_list: D019843:Archaeal Proteins; D026341:Minichromosome Maintenance 1 Protein; D013912:Threonine; D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D053488:DnaB Helicases
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