Optical imaging of molecular signatures in pulmonary inflammation.
Biomedical imaging has become an important tool in the study of "-omics" fields by allowing the noninvasive visualization of functional and molecular events using in vivo staining and reporter gene approaches. This capacity can go beyond the understanding of the genetic basis and phenotype of such respiratory conditions as acute bronchitis, adult respiratory distress syndrome (ARDS), chronic obstructive pulmonary disease (COPD), and asthma and investigate the development of disease and of therapeutic events longitudinally and in unperturbed environments. Herein, we show how the application of novel quantitative optical imaging methods, using transillumination and fluorescence molecular tomography (FMT), can allow visualization of pulmonary inflammation in small animals in vivo. The results confirm prior observations using a protease-sensitive probe. We discuss how this approach enables in vivo insights at the system level as to the dynamic role of proteases in respiratory pathophysiology and their potential as therapeutic targets. Overall, the proposed imaging method can be used with a significantly wider range of possible targets and applications in lung imaging.
Ntziachristos
Vasilis
V
Institute for Biological and Medical Imaging, Helmholtz Zentrum München and Technische Universität München, Munich, Germany. v.ntziachristos@tum.de
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005453:Fluorescence; D008163:Luminescent Measurements; D008171:Lung Diseases / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D057054:Molecular Imaging; D010447:Peptide Hydrolases / Q000627:therapeutic use; D014054:Tomography; D014172:Transillumination
D010447:Peptide Hydrolases
Med Phys. 2003 May;30(5):901-11
12772999
J Biomed Opt. 2006 Nov-Dec;11(6):064017
17212540
Nat Med. 2002 Jul;8(7):757-60
12091907
J Appl Physiol (1985). 2008 Mar;104(3):795-802
18202169
Cell Mol Biol (Noisy-le-grand). 2003 Sep;49(6):875-84
14656045
Phys Rev Lett. 2006 May 5;96(17):173903
16712298
IEEE Trans Med Imaging. 2004 Apr;23(4):492-500
15084074
Chem Immunol. 2000;78:72-80
12847720
J Opt Soc Am A Opt Image Sci Vis. 2004 Feb;21(2):231-41
14763766
Eur Respir J. 2003 Apr;21(4):567-73
12762337
Nat Biotechnol. 2005 Mar;23(3):313-20
15765087
Opt Lett. 2001 Jun 15;26(12):893-5
18040483
Circulation. 2007 Mar 20;115(11):1384-91
17339546
IEEE Trans Med Imaging. 2005 Oct;24(10):1377-86
16229423