Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress.
Increasing evidence indicates that carbon monoxide (CO) may protect against several diseases including sepsis. The ability of CO pre-treatment to provide good pre-conditioning against lipopolysaccharide (LPS)-induced injury was tested using an in vitro model of primary culture of porcine aortic endothelial cells (pAEC). pAEC were exposed to CO (250 ppm) or air for 1 h prior to the addition of LPS (10 microg/ml). Hsp70, HO-1, and Egr-1 protein levels were determined as well as vascular endothelial growth factor (VEGF) secretion after 4, 7, and 15 h. The effect of CO on LPS-induced apoptosis was also detected at 15 h. CO pre-treatment before the addition of LPS, significantly reduced LPS-induced apoptosis. LPS induced an increase in the level of VEGF in culture media after 7 and 15 h, and a larger increase was detected in CO pre-treated cells. In addition, CO pre-treatment reduced LPS-induced Hsp70, HO-1, and Egr-1 protein expression. In conclusion, CO treatment seems to provide a good pre-conditioning for the prevention of LPS-induced endothelial injury.
Bernardini
Chiara
C
Department of Veterinary Morphophysiology and Animal Production, DIMORFIPA, University of Bologna, Italy. chiara.bernardini5@unibo.it
Zannoni
Augusta
A
Bacci
Maria Laura
ML
Forni
Monica
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002248:Carbon Monoxide / Q000494:pharmacology*; D002478:Cells, Cultured; D019610:Cytoprotection; D051765:Early Growth Response Transcription Factors / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D013312:Stress, Physiological / Q000187:drug effects*; D013552:Swine; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D051765:Early Growth Response Transcription Factors; D008070:Lipopolysaccharides; D042461:Vascular Endothelial Growth Factor A; D002248:Carbon Monoxide
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