Postnatal expression of GAD67.
N-methyl-D-aspartate receptor blockade promotes apoptosis at postnatal day 7 (P7) and is linked to loss of glutamic acid decarboxylase 67 (GAD67) expression in older animals. To more fully appreciate this relationship we must first understand how GAD67 is regulated postnatally. Thus, the brains of P7, P14 and P21 rats were examined for expression of GAD67 protein and we found that levels of this GABAergic marker increased steadily with age, such that by P21 there was as much as a 6-fold increase compared to P7 animals and a 1.5- to 2-fold increase compared to P14 animals, depending on the region sampled. At P7, GAD67 was almost exclusively detected in puncta, with very few cell bodies displaying this marker. In contrast, at P14 and especially P21, both puncta and cell bodies were robustly labeled. Our data indicate that adult-like expression of GAD67 emerges quite late in the postnatal period.
Turner
Christopher P
CP
Neurobiology and Anatomy, Wake Forest University School of Medicine, 1 Medical Center Blvd, Winston-Salem, NC 27157-1010, USA. cpturner@wfubmc.edu
Ware
Emily
E
Stowe
Robert
R
DeBenedetto
Danielle
D
Walburg
Caroline
C
Lee
Andrew
A
Swanson
John
J
Lambert
Alexandra
A
Lyle
Melissa
M
Desai
Priyanka
P
Liu
Chun
C
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology*; D005968:Glutamate Decarboxylase / Q000096:biosynthesis*; D006179:Gyrus Cinguli / Q000201:enzymology; D017072:Neostriatum / Q000201:enzymology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013003:Somatosensory Cortex / Q000201:enzymology
D016194:Receptors, N-Methyl-D-Aspartate; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
Dev Psychobiol. 2007 Sep;49(6):606-18
17680608
Neurosci Lett. 2009 Sep 22;462(2):152-6
19596402
Neurochem Res. 1991 Mar;16(3):235-42
1780026
Eur J Neurosci. 1995 Mar 1;7(3):419-30
7773439
Brain Res Dev Brain Res. 1997 Nov 12;103(2):127-41
9427477
Neurotoxicol Teratol. 2007 Mar-Apr;29(2):264-72
17137750
Dev Psychobiol. 2008 Nov;50(7):665-79
18688810
Eur J Neurosci. 1994 Jun 1;6(6):983-97
7952285
Science. 1999 Jan 1;283(5398):70-4
9872743
J Comp Neurol. 1997 Jun 30;383(2):199-219
9182849
Exp Neurol. 2002 Nov;178(1):21-32
12460605
J Comp Neurol. 1995 Apr 24;355(1):81-92
7636016
J Neurosci. 2003 Jul 16;23(15):6315-26
12867516
Exp Brain Res. 2010 Mar;201(3):479-88
19885653
Dev Neurosci. 2007;29(3):213-31
16921238
J Neural Transm (Vienna). 2002;109(1):101-17
11793166
J Comp Neurol. 1992 Dec 22;326(4):501-26
1484122
Anat Embryol (Berl). 1979;157(1):1-14
92901
J Neurosci. 2006 Aug 23;26(34):8691-701
16928857
Arch Gen Psychiatry. 1995 Dec;52(12):998-1007
7492260
Neurosci Lett. 2009 Feb 6;450(3):292-5
19070650
Neuroscience. 2006 Sep 1;141(3):1351-63
16782280
Dev Biol. 1992 Sep;153(1):158-64
1516745
Anesthesiology. 2009 Apr;110(4):796-804
19293700
J Comp Neurol. 1989 Nov 8;289(2):213-27
2808764
Psychopharmacology (Berl). 2007 Jun;192(3):303-16
17340116
Neuropsychopharmacology. 2008 Sep;33(10):2442-55
18059437