[Oral immunization of Lugurus lugurus with the plasmid nanoemulsion of zona pellucida 3 to enhance infertility effects].
Immunocontraceptive technology is beginning to attract more attention for the control of wild mouse or other pest animals. Oral vaccination of animals and man, to provide effective mucosal and/or systemic immunity, is a good delivery system, but it remains poor bioavailablity, one of the most promising strategies to improve the oral delivery relies on polymeric nanoparticles carriers to encapsulate vaccine. Nanoemulsion was used as the carriers of oral immunization of Lugurus Lugurus zona pellucida 3, the immune response and antifertility potential of this vaccine is valuated. Nanoemulsion-encapsulated plasmid pcDNA3.0-Aat-COMP-lzp3-C3d3 (pACLC DNA vaccine) was prepared and evaluated the encapsulation efficiency by anion exchange chromatography. The mice were orally delivered with nanoemulsion-encapsulated plasmid, to detect specific IgG in serum and IgA in vaginal fluid by indirect ELISA, and to perform fertility experiment, to do histological analysis of immunized mice ovary. The results showed that the specific IgG and IgA antibody is elicited, the fertility rate is deceased at a certain extent. In conclusion, it is feasible that nanoemulsion is used as the carriers of oral immunization.
Zhang
Ai Lian
AL
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Zhuang
Shu Zhen
SZ
Wu
Dao Cheng
DC
Li
Yi Jie
YJ
Zhang
Fu Chun
FC
D000284:Administration, Oral; D000818:Animals; D003269:Contraception, Immunologic / Q000379:methods*; D004655:Emulsions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D007246:Infertility / Q000276:immunology*; D008099:Liver / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D049329:Nanostructures; D010957:Plasmids / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015044:Zona Pellucida / Q000276:immunology*
D004655:Emulsions; D019444:Vaccines, DNA