title: Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex.
abstract: The Nup84 complex constitutes a key building block in the nuclear pore complex (NPC). Here we present the crystal structure of one of its 7 components, Nup120, which reveals a beta propeller and an alpha-helical domain representing a novel fold. We discovered a previously unidentified interaction of Nup120 with Nup133 and confirmed the physiological relevance in vivo. As mapping of the individual components in the Nup84 complex places Nup120 and Nup133 at opposite ends of the heptamer, our findings indicate a head-to-tail arrangement of elongated Nup84 complexes into a ring structure, consistent with a fence-like coat for the nuclear pore membrane. The attachment site for Nup133 lies at the very end of an extended unstructured region, which allows for flexibility in the diameter of the Nup84 complex ring. These results illuminate important roles of terminal unstructured segments in nucleoporins for the architecture, function, and assembly of the NPC.
authors.affiliation: Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA.
authors.forename: Hyuk-Soo
authors.initials: HS
authors.lastname: Seo
authors.forename: Yingli
authors.initials: Y
authors.lastname: Ma
authors.forename: Erik W
authors.initials: EW
authors.lastname: Debler
authors.forename: Daniel
authors.initials: D
authors.lastname: Wacker
authors.forename: Stephan
authors.initials: S
authors.lastname: Kutik
authors.forename: Günter
authors.initials: G
authors.lastname: Blobel
authors.forename: André
authors.initials: A
authors.lastname: Hoelz
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014961:X-Ray Diffraction
chemical_list: C097740:NUP120 protein, S cerevisiae; C091271:NUP133 protein, S cerevisiae; C097894:NUP84 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins
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