Desferrioxamine shows different potentials for enhancing 5-aminolaevulinic acid-based photodynamic therapy in several cutaneous cell lines.
Protoporphyrin IX (PpIX) is the photosensitizer in 5-aminolaevulinic acid (ALA)-based photodynamic therapy (PDT). Its further bioconversion to heme requires iron and can be suppressed by iron chelators such as desferrioxamine (DFO). To investigate the effectiveness of DFO in enhancing PpIX-based PDT in skin tissue, we selected fibroblasts, HaCat cells and Hep-2 cells as targets co-cultured with ALA, that have different biological characteristics for PpIX conversion. Evaluated interventions included: (1) blank control (no ALA, no DFO); (2) DFO alone; (3) ALA alone; and (4) DFO in combination with ALA. Before photodynamic irradiation, cellular PpIX level and fluorescence were measured. After irradiation, cell death ratio was calculated and morphological changes in the cells were observed. The results showed that the content and photodynamic effects of cellular PpIX presented in the order Hep-2 cells > HaCat cells > fibroblasts, either co-cultured with ALA alone or with ALA plus DFO. DFO was found to have increasing effects on both PpIX level and cell death ratio in the same order. It was found that DFO had different potentials for augmenting ALA-PDT in these cutaneous cell lines. The cells proliferating more rapidly might be more susceptible to enhancement of DFO.
Yang
Jiabin
J
Department of Dermatology, The First Affiliated Hospital of Yangtze University, Jingzhou, China.
Xia
Yumin
Y
Liu
Xiaoming
X
Jiang
Shan
S
Xiong
Layuan
L
D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003676:Deferoxamine / Q000008:administration & dosage / Q000627:therapeutic use*; D004357:Drug Synergism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007502:Iron Chelating Agents / Q000008:administration & dosage / Q000627:therapeutic use; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D011524:Protoporphyrins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use; D012871:Skin Diseases / Q000188:drug therapy
D007502:Iron Chelating Agents; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; D003676:Deferoxamine
Photodermatol Photoimmunol Photomed. 2008 Oct;24(5):237-43
18811864
Lasers Surg Med. 2004;34(1):62-72
14755426
Folia Histochem Cytobiol. 2005;43(3):129-32
16201311
J Environ Pathol Toxicol Oncol. 2007;26(2):75-82
17725533
Photochem Photobiol Sci. 2004 Mar;3(3):263-7
14993942
Br J Dermatol. 2008 Dec;159(6):1245-66
18945319
Lasers Surg Med. 2008 Feb;40(2):153-8
18306163
Eur J Pharm Biopharm. 2006 Jul;63(3):295-309
16682177
J Biomed Opt. 2008 Mar-Apr;13(2):024014
18465977
Chin Med J (Engl). 2004 Jun;117(6):922-6
15198900
J Cardiovasc Pharmacol. 2005 Jun;45(6):539-44
15897780
Int J Biochem Cell Biol. 2005 Feb;37(2):272-6
15474973
Photochem Photobiol Sci. 2007 Dec;6(12):1268-74
18046481
Photochem Photobiol. 2007 May-Jun;83(3):766-73
17576385
Photochem Photobiol Sci. 2008 Mar;7(3):283-9
18389144
Exp Dermatol. 2007 Aug;16(8):651-60
17620092
World J Gastroenterol. 2008 Apr 14;14(14):2174-8
18407590
J Invest Dermatol. 2006 Dec;126(12):2679-86
16841035
Exp Dermatol. 2004 Jul;13(7):445-51
15217365
J Pharm Pharmacol. 1999 Feb;51(2):131-4
10217310
Lasers Surg Med. 2005 Jun;36(5):398-408
15856508
Exp Oncol. 2005 Mar;27(1):81-3
15812364
Photochem Photobiol. 2002 Apr;75(4):418-23
12003133
Pharm Res. 2006 Feb;23(2):360-6
16341572
Br J Dermatol. 2008 Jul;159(1):61-7
18489589
Cancer. 2001 Dec 15;92(12):3093-110
11753989
J Environ Pathol Toxicol Oncol. 2006;25(1-2):403-9
16566731
J Gastroenterol. 2007 Jun;42(6):475-84
17671763
J Environ Pathol Toxicol Oncol. 2007;26(2):89-103
17725535
Biochem Pharmacol. 2007 Nov 1;74(9):1401-12
17714696
J Natl Compr Canc Netw. 2007 May;5(5):531-40
17509255
Dermatology. 2009;218(3):193-202
19077380
J Photochem Photobiol B. 2004 Jan 23;73(1-2):67-78
14732253