Conformation, activity and proteolytic stability of acid phosphatase on clay minerals and soil colloids from an Alfisol.
The present study was carried out to investigate the conformation, enzymatic activity and proteolytic stability of acid phosphatase on montmorillonite, kaolinite and soil colloids from an Alfisol by means of circular dichroism (CD) spectroscopy, isothermal titration microcalorimetry (ITC) and biochemical assay, respectively. The results showed that the secondary structure of phosphatase was changed from disordered type to ordered form during adsorption/desorption cycle, organic substance and 2:1-clay mineral in Brown Soil benefited the formation of ordered structure. Enzymatic activity of phosphatase was inhibited while the proteolytic stability was promoted after the interaction with active particles from permanent charge soil. The decrease of enzymatic activity and the increase of proteolytic stability resulted by montmorillonite and organic colloid were both greater than that by kaolinite and inorganic colloid, which was in consistent with the extent of structural change induced by different colloid particles. Thus, one of the most significant factors responsible for the variation of enzymatic activity and proteolytic stability might be the hiding or even damage of active sites and the irrecognition of cleavage sites in enzyme molecules induced by the formation of ordered structure. The information obtained in this study is of crucial significance for the understanding of the behavior and fate of extracellular enzymes in soils with permanent charges.
Huang
Qiaoyun
Q
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. qyhuang@mail.hzau.edu.cn
Zhu
Jun
J
Qiao
Xueqing
X
Cai
Peng
P
Rong
Xingmin
X
Liang
Wei
W
Chen
Wenli
W
D000135:Acid Phosphatase / Q000737:chemistry* / Q000378:metabolism*; D000538:Aluminum Silicates / Q000378:metabolism*; D001546:Bentonite; D055162:Biocatalysis; D002942:Circular Dichroism; D000077215:Clay; D003102:Colloids; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006358:Hot Temperature; D007616:Kaolin; D008903:Minerals; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D012987:Soil; D011198:Solanum tuberosum / Q000201:enzymology*; D013997:Time Factors
D000538:Aluminum Silicates; D003102:Colloids; D004800:Enzymes, Immobilized; D008903:Minerals; D012987:Soil; D001546:Bentonite; D007616:Kaolin; D000135:Acid Phosphatase; D000077215:Clay