title: Induction of tetraploids from petiole explants through colchicine treatments in Echinacea purpurea L.
abstract: Petiole explants were obtained from in vitro grown diploid (2x = 22) Echinacea purpurea plantlets. Shoots were regenerated by culturing the explants on MS basal medium containing 0.3 mg/L benzyladenine (BA), 0.01 mg/L naphthaleneacetic acid (NAA) and four concentrations (30, 60, 120, and 240 mg/L) of colchicine for 30 days, or 120 mg/L of colchicine for various durations (7, 14, 21, and 28 days). The regenerated shoots were induced to root on MS basal medium with 0.01 mg/L NAA, and then the root-tips of the regenerated shoots were sampled for count of chromosome number. It was found that a treatment duration of >7 days was necessary for induction of tetraploid (4x = 44) shoots, and treatment with 120 mg/L colchicine for 28 days was the most efficient for induction of tetraploids, yielding 23.5% of tetraploids among all the regenerated shoots. Chimeras were observed in almost all the treatments. However, the ratio of tetraploid to diploid cells in a chimeric plant was usually low. In comparison with diploid plants, tetraploid plants in vitro had larger stomata and thicker roots with more root branches, and had prominently shorter inflorescence stalk when mature.
authors.affiliation: Genetic Engineering Laboratory, College of Life Sciences, South China Agricultural University, Guangzhou, China.
authors.forename: Dahanayake
authors.initials: D
authors.lastname: Nilanthi
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Chen
authors.forename: Fu-Cheng
authors.initials: FC
authors.lastname: Zhao
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D003078:Colchicine / Q000494:pharmacology*; D003470:Culture Media; D004171:Diploidy; D020900:Echinacea / Q000166:cytology / Q000187:drug effects* / Q000235:genetics*; D018515:Plant Leaves / Q000187:drug effects* / Q000235:genetics*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D054046:Plant Stomata / Q000033:anatomy & histology / Q000187:drug effects; D030821:Plants, Genetically Modified; D011123:Polyploidy; D012038:Regeneration / Q000187:drug effects; D013997:Time Factors
chemical_list: D003470:Culture Media; D003078:Colchicine
references.citation: Cochrane Database Syst Rev. 2000;(2):CD000530
references.pmid: 10796553
references.citation: Plant Cell Rep. 1998 Jul;17(10):799-803
references.pmid: 30736595
references.citation: Plant Cell Rep. 2005 Dec;24(11):671-6
references.pmid: 16094528
references.citation: Plant Cell Rep. 2000 Jan;19(3):274-278
references.pmid: 30754907
references.citation: Arch Fam Med. 1998 Nov-Dec;7(6):541-5
references.pmid: 9821828
references.citation: Biotechnol Lett. 2007 Aug;29(8):1179-82
references.pmid: 17589811
references.citation: J Pharm Pharmacol. 2005 Aug;57(8):929-54
references.pmid: 16102249