Circulating level of microparticles and their correlation with arterial elasticity and endothelium-dependent dilation in patients with type 2 diabetes mellitus.
OBJECTIVE
This study was designed to measure the circulating level of microparticles (MP) in patients with type 2 diabetes mellitus versus healthy volunteers and to evaluate their correlation with arterial elasticity and endothelium-dependent dilation.


METHODS
Flow cytometry was used to measure the circulating levels of MP, including annexinV+MP, platelet-derived microparticles (PMP), leukocyte-derived microparticles (LMP) and endothelial microparticles (EMP), in 63 patients with type 2 diabetes mellitus and 29 healthy volunteers. Brachial ankle pulse wave velocity (baPWV) and endothelium-dependent flow-mediated dilation (FMD) of the brachial artery were also measured.


RESULTS
The levels of annexinV+MP, PMP, LMP, CD31+/CD42-EMP and CD51+EMP increased significantly in diabetic patients compared with healthy controls (P<0.001). Correlation analysis showed that HbA(1c) was positively correlated to CD31+/CD42-EMP (r(s)=0.337, P=0.008) and CD51+EMP (r(s)=0.266, P=0.038). FMD in diabetic patients was significantly lower than that in healthy individuals (P=0.007). FMD was negatively correlated to CD31+/CD42-EMP (r(s)=-0.441, P=0.008) and CD51+EMP (r(s)=-0.405, P=0.016). baPWV level in diabetic patients was significantly higher than that in healthy individuals (P<0.001). baPWV was positively correlated to CD31+/CD42-EMP (r(s)=0.497, P<0.001) and CD51+EMP (r(s)=0.428, P=0.001). Multiple regression analysis indicated that EMP was an independent risk factor of FMD and baPWV.


CONCLUSIONS
The circulating level of microparticles increases in patients with type 2 diabetes. The level of endothelial microparticles is closely associated with vascular dysfunction.
Feng
Bo
B
Department of Endocrinology, East Hospital, Tongji University, 150 Jimo Road, Shanghai, China. fengbo2010@sohu.com
Chen
Yinghua
Y
Luo
Yun
Y
Chen
Ming
M
Li
Xu
X
Ni
Yafang
Y
D001158:Arteries / Q000503:physiopathology*; D055252:Cell-Derived Microparticles; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology*; D004548:Elasticity; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged