title: Validation of radioimmunoassay screening methods for beta-agonists in bovine liver according to Commission Decision 2002/657/EC.
abstract: Validation studies were carried out on a multi-residue screening method for anilinic type beta-agonists (clenbuterol, mabuterol, brombuterol, cimaterol, cimbuterol, mapenterol, clenpenterol) and a method for the phenolic type beta-agonist, salbutamol, in bovine liver. The validation was performed according to the European Union Commission Decision 2002/657/EC (European Commission 2002), which establishes criteria and procedures for the determination of parameters such as the detection capability (CCbeta), specificity, stability of standard solutions and stability of the analyte in matrix. CCbeta values for the eight target compounds were between 0.25 and 0.5 microg kg(-1). The stability of standard solutions and analytes in matrix and the specificity of the antibody were characterized. The methods are applicable for qualitative screening of beta-agonists for regulatory programmes according to European Union performance requirements, or as a semi-quantitative research tool for known target compounds.
authors.affiliation: Microbioticos Laboratories, Campinas, Brazil. rodrigo@microbioticos.com
authors.forename: R H M M
authors.initials: RH
authors.lastname: Granja
authors.forename: A M
authors.initials: AM
authors.lastname: Montes Niño
authors.forename: F
authors.initials: F
authors.lastname: Rabone
authors.forename: R E
authors.initials: RE
authors.lastname: Montes Niño
authors.forename: A
authors.initials: A
authors.lastname: Cannavan
authors.forename: A Gonzalez
authors.initials: AG
authors.lastname: Salerno
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods / Q000592:standards; D005506:Food Contamination / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D011786:Quality Control; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000318:Adrenergic beta-Agonists