Expressive proteomics profile changes of injured human brain cortex due to acute brain trauma.
OBJECTIVE
To find the expressive proteomics changes in damaged human brain cortex after traumatic brain injury (TBI).


METHODS
By rapid high-throughput and precise proteomic techniques, the traumatic injured human frontal cortexes were compared with non-trauma controls.


RESULTS
On 2-DE PAGE, 138 protein spots were found significantly different on expressive level of quantitative mature. Most of these proteins expressed in a fluctuant fashion within 18 hours after trauma, with mean levels lower than control. Eighty-two protein spots were identified by MALDI-MS TOF, which were products of 71 proteins and could be grouped into 10 categories based on possible functions: cytoskeleton (n = 10), metabolism (n = 13), electron transport (n = 8), signalling transduction (n = 4), stress response (n = 6), protein synthesis and turnover (n = 8), transporter (n = 5), cell cycle (n = 1), other (n = 8) and unknown (n = 9).


CONCLUSIONS
After traumatic brain injury, there are significant proteins expressing changes in damaged brain tissue. These proteins may play a critical role in TBI. Although some of these proteins functions are not fully understood, they may become novel biomarkers and novel therapy targets in the future.
Yang
Xinyu
X
Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, PR China.
Yang
Shuyuan
S
Wang
Jie
J
Zhang
Xuemin
X
Wang
Chen
C
Hong
Guoliang
G
D000328:Adult; D000368:Aged; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012016:Reference Values; D055815:Young Adult
D020543:Proteome