Combined use of chiral ionic liquid and CD for MEKC: Part II. Determination of binding constants.
A competitive inhibition mechanism is proposed to investigate the interactions among 2,3,6-tri-O-methyl-beta-CD (TM-beta-CD), cationic ionic liquid type surfactants, N-undecenoxy-carbonyl-L-leucinol bromide (L-UCLB) and profens using affinity CE. The apparent binding constant of TM-beta-CD to L-UCLB was estimated by nonlinear and linear plotting methods. The binding constants of one representative profen (e.g. fenoprofen) to TM-beta-CD and L-UCLB were estimated by a secondary plotting approach. The R- and S-fenoprofens have different binding constant values, resulting in the enantioseparation due to the synergistic effect of the two chiral selectors, TM-beta-CD and L-UCLB.
Wang
Bin
B
Department of Chemistry, Center of Biotechnology and Drug Design, Georgia State University, Atlanta, GA 30303, USA.
He
Jun
J
Bianchi
Victoria
V
Shamsi
Shahab A
SA
D001667:Binding, Competitive; D002412:Cations / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry*; D007700:Kinetics; D016014:Linear Models; D008956:Models, Chemical; D017711:Nonlinear Dynamics; D010666:Phenylpropionates / Q000302:isolation & purification*; D013237:Stereoisomerism; D013501:Surface-Active Agents / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D002412:Cations; D052578:Ionic Liquids; D010666:Phenylpropionates; D013501:Surface-Active Agents; D047392:beta-Cyclodextrins; C000609658:heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin
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