title: Effects of platelet-rich plasma gel on skin healing in surgical wound in horses.
abstract: OBJECTIVE
  To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine.
  
  
  METHODS
  To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair.
  
  
  RESULTS
  The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation.
  
  
  CONCLUSIONS
  Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine.
authors.affiliation: Department of Veterinary Medicine, Surgery and Anesthesiology, Faculty of Veterinary Medicine and Animal Science, UFMS, Mato Grosso do Sul, Brazil. derossi@nin.ufms.br
authors.forename: Rafael
authors.initials: R
authors.lastname: DeRossi
authors.forename: Anna Carolina Anciliero de Oliveira
authors.initials: AC
authors.lastname: Coelho
authors.forename: Gisele Silveira de
authors.initials: GS
authors.lastname: Mello
authors.forename: Fabrício Oliveira
authors.initials: FO
authors.lastname: Frazílio
authors.forename: Cássia Rejane Brito
authors.initials: CR
authors.lastname: Leal
authors.forename: Gilberto Gonçalves
authors.initials: GG
authors.lastname: Facco
authors.forename: Karine Bonucielli
authors.initials: KB
authors.lastname: Brum
mesh_terms: D000818:Animals; D001706:Biopsy; D002125:Calcium Gluconate / Q000627:therapeutic use*; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D005260:Female; D005782:Gels; D006490:Hemostatics / Q000627:therapeutic use*; D006736:Horses; D008297:Male; D053657:Platelet-Rich Plasma; D012867:Skin / Q000473:pathology / Q000503:physiopathology; D013917:Thrombin / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014947:Wounds and Injuries / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*
chemical_list: D005782:Gels; D006490:Hemostatics; D013917:Thrombin; D002125:Calcium Gluconate