title: Identification of a novel vimentin promoter and mRNA isoform.
abstract: The intermediate filament protein vimentin is involved in a variety of cellular functions both during the normal developmental processes and in human malignancies. We here describe the identification of an alternative vimentin transcript initiating upstream for the canonical vimentin gene promoter and spliced using the vimentin promoter sequence as an intron. Expression analysis showed that the alternative vimentin promoter had the same expression profile as the canonical vimentin gene promoter. The presented data suggest that alternative promoter usage and alternative splicing could be regulatory mechanisms participating in vimentin gene regulation.
authors.affiliation: Department of Human Genetics, University of Aarhus, Aarhus, Denmark.
authors.forename: Zhangle
authors.initials: Z
authors.lastname: Zhou
authors.forename: Søren
authors.initials: S
authors.lastname: Kahns
authors.forename: Anders Lade
authors.initials: AL
authors.lastname: Nielsen
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014746:Vimentin / Q000235:genetics* / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D014746:Vimentin
references.citation: Neurochem Res. 1999 May;24(5):709-14
references.pmid: 10344602
references.citation: Bioessays. 1998 Jan;20(1):79-86
references.pmid: 9504050
references.citation: Eur J Biochem. 1992 Dec 1;210(2):509-19
references.pmid: 1459133
references.citation: Genome Res. 2006 Jan;16(1):55-65
references.pmid: 16344560
references.citation: Exp Cell Res. 2007 Jun 10;313(10):2050-62
references.pmid: 17512929
references.citation: Cancer Res. 1994 Jun 15;54(12):3092-5
references.pmid: 8205522
references.citation: Nature. 2007 Feb 8;445(7128):666-70
references.pmid: 17237763
references.citation: Nat Genet. 2006 Jun;38(6):626-35
references.pmid: 16645617
references.citation: Oncogene. 2004 Jan 8;23(1):168-78
references.pmid: 14712222
references.citation: Nature. 2005 Aug 11;436(7052):876-80
references.pmid: 15988478
references.citation: Mol Cancer Ther. 2009 Mar;8(3):499-508
references.pmid: 19276168
references.citation: J Biol Chem. 2002 Aug 16;277(33):29983-91
references.pmid: 12058025
references.citation: Gene. 1999 Apr 1;230(1):111-20
references.pmid: 10196480
references.citation: Gene. 1999 Jul 22;235(1-2):69-75
references.pmid: 10415334
references.citation: Gene. 1996 Feb 12;168(2):261-6
references.pmid: 8654956
references.citation: Differentiation. 1999 Nov;65(3):171-80
references.pmid: 10631814
references.citation: J Cell Sci. 1999 Dec;112 ( Pt 24):4615-25
references.pmid: 10574710
references.citation: Annu Rev Biochem. 2004;73:749-89
references.pmid: 15189158
references.citation: J Neurosci Res. 1999 May 1;56(3):219-28
references.pmid: 10336251
references.citation: J Cell Sci. 1994 Jun;107 ( Pt 6):1593-607
references.pmid: 7962200
references.citation: Curr Protoc Mol Biol. 2005 Feb;Chapter 21:Unit 21.3
references.pmid: 18265358
references.citation: Nature. 2007 Aug 2;448(7153):553-60
references.pmid: 17603471
references.citation: Am J Pathol. 1997 Feb;150(2):483-95
references.pmid: 9033265
references.citation: Q Rev Biophys. 1999 May;32(2):99-187
references.pmid: 10845237
references.citation: Cells Tissues Organs. 2007;185(1-3):191-203
references.pmid: 17587825
references.citation: Mol Cell Biol. 1987 Nov;7(11):3908-15
references.pmid: 3431546
references.citation: J Muscle Res Cell Motil. 2002;23(5-6):535-40
references.pmid: 12785103
references.citation: Cell. 2007 Jul 13;130(1):77-88
references.pmid: 17632057
references.citation: J Biol Chem. 2003 Feb 28;278(9):7422-30
references.pmid: 12493776
references.citation: Genes Cells. 2007 Aug;12(8):905-18
references.pmid: 17663720
references.citation: Pathol Biol (Paris). 1998 Jan;46(1):39-45
references.pmid: 9769935
references.citation: Int J Dev Biol. 1995 Jun;39(3):443-57
references.pmid: 7577435
references.citation: Nucleic Acids Res. 2007;35(22):7636-50
references.pmid: 17981838
references.citation: Nucleic Acids Res. 1988 Aug 25;16(16):8057-76
references.pmid: 3419902
references.citation: Nat Genet. 2007 Mar;39(3):311-8
references.pmid: 17277777
references.citation: Gene. 1988;62(1):7-16
references.pmid: 3371665
references.citation: Exp Cell Res. 1986 Apr;163(2):509-17
references.pmid: 3956586
references.citation: Transgenic Res. 2009 Aug;18(4):545-58
references.pmid: 19184503
references.citation: Cell Growth Differ. 1994 Aug;5(8):839-46
references.pmid: 7986748
references.citation: Mol Psychiatry. 2003 Sep;8(9):786-96
references.pmid: 12931206
references.citation: FEBS Lett. 2000 Mar 24;470(2):198-202
references.pmid: 10734233