Analgesic and anti-inflammatory activities of a water extract of Trachelospermum jasminoides (Apocynaceae).
OBJECTIVE
This study investigated the analgesic and anti-inflammatory effects of a water extract of Trachelospermum jasminoides (WET) in ICR mice.


METHODS
In HPLC analysis, the fingerprint chromatogram of WET was established. Acetic acid-induced writhing response and formalin-induced pain were examined the analgesics effects of WET. WET on lambda-Carrageenan(carr)-induced paw edema was performed. We investigate the anti-inflammatory mechanism of WET via studies of the activities of glutathione peroxidase (GPx), glutathione reductase (GRx) in the liver and the levels of malondialdehyde (MDA) and nitrite oxide (NO) in the edema paw. Serum NO and TNF-alpha were also measured.


RESULTS
The fingerprint chromatogram of WET was established through HPLC analysis, and implies that WET contains the active ingredient gallic acid, chlorgenic acid, caffeic acid, taxifolin, isoquercitrin and quercetin. WET significantly inhibited the numbers of acetic acid-induced writhing responses and the formalin-induced pain in the late phase. In the anti-inflammatory test, WET inhibited the development of paw edema induced by carr. WET decreased the paw edema at the third, fourth and fifth hour after carr administration, and increased the activities of SOD, GPx and GRx in the liver tissue and decreased the MDA level in the edema paw at the third hour after carr injection. WET decreased the level of NO in edematous paw tissue and in serum level, and diminished the level of serum TNF-alpha at the fifth hour after carr injection.


CONCLUSIONS
These results demonstrated that WET is an effective anti-inflammatory agent in carr-induced inflammation. WET probably exerts anti-inflammatory effects by suppressing TNF-alpha and NO. The anti-inflammatory mechanism of WET might be related to the decrease in the level of MDA in the edema paw via increasing the activities of SOD, GPx and GRx in the liver.
Sheu
Ming-Jyh
MJ
School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. soybean13mtdtw@gmail.com
Chou
Pei-Yu
PY
Cheng
Hsu-Chen
HC
Wu
Chieh-Hsi
CH
Huang
Guan-Jhong
GJ
Wang
Bor-Sen
BS
Chen
Jwo-Sheng
JS
Chien
Yi-Chung
YC
Huang
Ming-Hsing
MH
D019342:Acetic Acid; D000700:Analgesics / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D029062:Apocynaceae / Q000737:chemistry*; D001522:Behavior, Animal / Q000187:drug effects*; D002351:Carrageenan; D004487:Edema / Q000188:drug therapy; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D005557:Formaldehyde; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000188:drug therapy; D010636:Phenols / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D005557:Formaldehyde; D008315:Malondialdehyde; D002351:Carrageenan; D019342:Acetic Acid