Proteomics meets blood banking: identification of protein targets for the improvement of platelet quality.
Proteomics has brought new perspectives to the fields of hematology and transfusion medicine in the last decade. The steady improvement of proteomic technology is propelling novel discoveries of molecular mechanisms by studying protein expression, post-translational modifications and protein interactions. This review article focuses on the application of proteomics to the identification of molecular mechanisms leading to the deterioration of blood platelets during storage - a critical aspect in the provision of platelet transfusion products. Several proteomic approaches have been employed to analyse changes in the platelet protein profile during storage and the obtained data now need to be translated into platelet biochemistry in order to connect the results to platelet function. Targeted biochemical applications then allow the identification of points for intervention in signal transduction pathways. Once validated and placed in a transfusion context, these data will provide further understanding of the underlying molecular mechanisms leading to platelet storage lesion. Future aspects of proteomics in blood banking will aim to make use of protein markers identified for platelet storage lesion development to monitor proteome changes when alterations such as the use of additive solutions or pathogen reduction strategies are put in place in order to improve platelet quality for patients.
Schubert
Peter
P
Canadian Blood Services, Centre for Blood Research and the Department of Pathology and Laboratory Medicine, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada.
Devine
Dana V
DV
D015415:Biomarkers / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D001771:Blood Banks / Q000639:trends*; D001774:Blood Chemical Analysis / Q000379:methods / Q000639:trends; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001793:Blood Preservation / Q000009:adverse effects / Q000379:methods / Q000592:standards; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D040901:Proteomics / Q000379:methods*; D011786:Quality Control; D000094902:Blood Banking / Q000379:methods*
D015415:Biomarkers; D001798:Blood Proteins