title: EBV-positive human sera contain antibodies against the EBV BMRF-2 protein.
abstract: We previously showed that the EBV glycoprotein BMRF-2 contains a functional integrin-binding Arg-Gly-Asp (RGD) domain that plays an important role in viral infection and cell-to-cell spread of progeny virions in oral epithelial cells. In this study, we found that EBV-seropositive human sera contain antibodies against BMRF-2. The inhibitory effect of EBV-positive sera on EBV infection of oral epithelial cells was substantially reduced by pre-incubation of serum samples with the BMRF-2 RGD peptide, suggesting that anti-BMRF-2 human antibodies possess neutralizing activity. EBV-specific sera reacted strongly with the BMRF-2 extracellular domain (170-213 aa) containing the RGD motif, whereas they reacted only weakly or not at all with a mutated form of the BMRF-2 extracellular domain containing AAA instead of RGD. These data indicate that RGD motif of BMRF-2 is part of an immunodominant antigenic determinant within the extracellular domain of BMRF-2 that may contribute to EBV neutralization during EBV reactivation.
authors.affiliation: Department of Medicine, University of California, San Francisco, CA 94143-0654, USA.
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Xiao
authors.forename: Joel M
authors.initials: JM
authors.lastname: Palefsky
authors.forename: Rossana
authors.initials: R
authors.lastname: Herrera
authors.forename: Carl
authors.initials: C
authors.lastname: Sunshine
authors.forename: Sharof M
authors.initials: SM
authors.lastname: Tugizov
mesh_terms: D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D004847:Epithelial Cells / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D009500:Neutralization Tests; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; C109482:BMRF-2 protein, Human herpesvirus 4; D008562:Membrane Glycoproteins; D014764:Viral Proteins
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