title: A photochromic photoreceptor from a eubacterium.
abstract: Sensory rhodopsin I (SRI) is one of the most interesting photo- sensory receptors because of its function in using the photochromic reaction to mediate opposing signals which depend on the color of light. It was initially thought that SRI exists only in the archaea, but we recently reported for the first time a newly functional SRI from a eubacterium, Salinibacter ruber (SrSRI). The amino acid sequence of SrSRI shows 43% identity with the well-known SRI (HsSRI) and contains most of the amino acid residues identified as necessary for SRI function. The photochemical properties of SrSRI are similar to those of HsSRI. In addition, SrSRI is a highly stable protein, even in dilute salt conditions. Thus, SrSRI could be a key protein for characterizing its association with the SrSRI transducer protein, SrHtrI, and for elucidating structural changes of SRI and HtrI that occur during their function. Recently, new approaches to manipulate cellular functions with rhodopsins have been established. SRI can activate and deactivate a kinase, CheA, by the photochromic reaction. Kinases are key molecules for signal transduction in various organisms, and SRI could potentially manipulate their cellular functions.
authors.affiliation: Division of Biological Science; Graduate School of Science.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Suzuki
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kitajima-Ihara
authors.forename: Yuji
authors.initials: Y
authors.lastname: Furutani
authors.forename: Kunio
authors.initials: K
authors.lastname: Ihara
authors.forename: Hideki
authors.initials: H
authors.lastname: Kandori
authors.forename: Michio
authors.initials: M
authors.lastname: Homma
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sudo
references.citation: Trends Microbiol. 2006 Nov;14(11):463-9
references.pmid: 17008099
references.citation: Mol Microbiol. 2003 Mar;47(6):1513-22
references.pmid: 12622809
references.citation: Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8034-9
references.pmid: 10393943
references.citation: Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10131-6
references.pmid: 11504917
references.citation: Trends Microbiol. 2006 Nov;14(11):480-7
references.pmid: 17005405
references.citation: Mol Microbiol. 1998 Jun;28(6):1051-8
references.pmid: 9680197
references.citation: Proc Natl Acad Sci U S A. 1986 Oct;83(19):7272-6
references.pmid: 3463965
references.citation: Curr Opin Struct Biol. 2002 Aug;12(4):516-22
references.pmid: 12163076
references.citation: Proc Natl Acad Sci U S A. 1982 Oct;79(20):6250-4
references.pmid: 6959114
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8689-94
references.pmid: 12060707
references.citation: Annu Rev Biophys Biomol Struct. 1997;26:223-58
references.pmid: 9241419
references.citation: Science. 1995 Jul 7;269(5220):73-5
references.pmid: 7604281
references.citation: Nature. 1984 Dec 6-12;312(5994):509-13
references.pmid: 6504161
references.citation: Biochemistry. 2008 Mar 4;47(9):2875-83
references.pmid: 18220358
references.citation: EMBO J. 1995 Sep 1;14(17):4249-57
references.pmid: 7556066
references.citation: J Biol Chem. 2008 Aug 29;283(35):23533-41
references.pmid: 18566451
references.citation: Nat Neurosci. 2008 Jun;11(6):631-3
references.pmid: 18432196
references.citation: Biochemistry (Mosc). 2001 Nov;66(11):1197-209
references.pmid: 11743865
references.citation: Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18147-52
references.pmid: 16330755
references.citation: Science. 2000 Sep 15;289(5486):1902-6
references.pmid: 10988064
references.citation: Biophys J. 2006 Aug 15;91(4):1471-9
references.pmid: 16731558
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16129-34
references.pmid: 17050685
references.citation: Nature. 2007 Apr 5;446(7136):633-9
references.pmid: 17410168
references.citation: J Biol Chem. 2007 May 25;282(21):15550-8
references.pmid: 17387174
references.citation: Annu Rev Cell Dev Biol. 1997;13:457-512
references.pmid: 9442881
references.citation: Photochem Photobiol Sci. 2006 Jun;5(6):538-46
references.pmid: 16761082
references.citation: Science. 2002 Jun 28;296(5577):2395-8
references.pmid: 12089443
references.citation: Proc Natl Acad Sci U S A. 1973 Oct;70(10):2853-7
references.pmid: 4517939
references.citation: J Biol Chem. 2006 Nov 10;281(45):34239-45
references.pmid: 16968701