Diffusive movement of processive kinesin-1 on microtubules.
The processive motor kinesin-1 moves unidirectionally toward the plus end of microtubules. This process can be visualized by total internal reflection fluorescence microscopy of kinesin bound to a carboxylated quantum dot (Qdot), which acts both as cargo and label. Surprisingly, when kinesin is bound to an anti-HIS Qdot, it shows diffusive movement on microtubules, which decreased in favor of processive runs with increasing salt concentration. This observation implies that kinesin movement on microtubules is governed by its conformation, as it is well established that kinesin undergoes a salt-dependent transition from a folded (inactive) to an extended (active) molecule. A truncated kinesin lacking the last 75 amino acids (kinesin-Delta C) showed both processive and diffusive movement on microtubules. The extent of each behavior depends on the relative amounts of ADP and ATP, with purely diffusive movement occurring in ADP alone. Taken together, these data imply that folded kinesin.ADP can exist in a state that diffuses along the microtubule lattice without expending energy. This mechanism may facilitate the ability of kinesin to pick up cargo, and/or allow the kinesin/cargo complex to stay bound after encountering obstacles.
Lu
Hailong
H
Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA.
Ali
M Yusuf
MY
Bookwalter
Carol S
CS
Warshaw
David M
DM
Trybus
Kathleen M
KM
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004058:Diffusion; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D009068:Movement; D011487:Protein Conformation; D017510:Protein Folding; D021381:Protein Transport; D045663:Quantum Dots
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C475469:Kif5b protein, mouse; D016547:Kinesins
J Biol Chem. 2007 Sep 14;282(37):27192-27197
17640878
Nat Cell Biol. 2001 Apr;3(4):425-8
11283618
J Cell Biol. 2008 Aug 11;182(3):421-8
18678707
Nat Cell Biol. 2000 May;2(5):257-60
10806475
Nat Cell Biol. 1999 Sep;1(5):293-7
10559942
J Cell Biol. 2007 Jan 1;176(1):11-7
17200414
Nano Lett. 2006 Jul;6(7):1491-5
16834436
Nature. 2006 May 4;441(7089):115-9
16672973
Proc Natl Acad Sci U S A. 2008 Jul 1;105(26):8938-43
18579780
Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):640-5
10639132
J Cell Biol. 2008 May 5;181(3):411-9
18443223
Curr Opin Cell Biol. 2009 Feb;21(1):68-73
19185482
Biophys J. 2000 Apr;78(4):1955-64
10733974
Biophys J. 2007 Jun 15;92(12):4137-44
17400704
Biochemistry. 1994 Feb 22;33(7):1951-60
8110800
Curr Opin Cell Biol. 2007 Feb;19(1):31-5
17184986
PLoS Biol. 2009 Mar 31;7(3):e72
19338388
J Cell Biol. 2007 Jan 1;176(1):51-63
17200416
Proc Natl Acad Sci U S A. 1988 Sep;85(17):6314-8
2970638
Trends Biochem Sci. 2004 Jun;29(6):301-9
15276184
Biophys J. 2001 Nov;81(5):2838-50
11606295
Exp Cell Res. 2008 Nov 15;314(19):3563-9
18848542
J Biol Chem. 2008 Dec 26;283(52):36465-73
18984586
J Biol Chem. 1992 Apr 25;267(12):8696-701
1569110
Curr Biol. 2002 Feb 19;12(4):301-6
11864570
Biophys J. 1997 Oct;73(4):2012-22
9336196
J Cell Biol. 2008 Aug 11;182(3):417-9
18678712
J Cell Biol. 2000 Nov 27;151(5):1081-92
11086009
Nat Chem Biol. 2006 Sep;2(9):480-5
16892050
Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1183-8
14734813
Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4332-6
17360524
J Neurochem. 2009 May;109(3):683-93
19250341
J Biol Chem. 1997 Jan 10;272(2):724-30
8995356
Science. 2003 Jun 27;300(5628):2061-5
12791999
Curr Biol. 2008 Jan 22;18(2):152-7
18207739
Biophys J. 2005 Nov;89(5):3223-34
16100283