title: [Fetal bovine serum enhances expression of PEDF in epidermal keratinocytes and dermal fibroblasts].
abstract: OBJECTIVE
  To investigate the effect of fetal bovine serum (FBS) on expression of pigment epithelium-derived factor (PEDF) in normal epidermal keratinocytes and dermal fibroblasts.
  
  
  METHODS
  Keratinocytes and fibroblasts were incubated with 10% FBS. PEDF protein level in the cells was determined by immunofluorescence and Western blot.
  
  
  RESULTS
  PEDF was localized mostly in the cytoplasm,while some in the nuclei. The distribution of PEDF in cytoplasm was in a granular pattern. 10% FBS increased the expression of PEDF both in keratinocytes and fibroblasts,but histamine and Phorbol 12-myristate 13-acetate (PMA) did not interfere the distribution of PEDF in cells.
  
  
  CONCLUSIONS
  10% FBS can upregulate expression of PEDF in epidermal keratinocytes and dermal fibroblasts.
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Xiao-yong
authors.initials: XY
authors.lastname: MAN
authors.forename: Yong-gang
authors.initials: YG
authors.lastname: YAO
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: YANG
authors.forename: Sui-qing
authors.initials: SQ
authors.lastname: CAI
authors.forename: Min
authors.initials: M
authors.lastname: ZHENG
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D044967:Serum / Q000502:physiology*; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D015854:Up-Regulation; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor