The efficacy of new colchicine derivatives and viability of the T-Lymphoblastoid cells in three-dimensional culture using 19F MRI and HPLC-UV ex vivo.
The paper describes ex vivo applications of colchicine derivatives for the treatment of human T-Lymphoblastoid (CEM) cells. Moreover, the role of the substitutions of ring A at C-1 and C-7 side chain of colchicine analogues was probed by the synthesis and examination of their effects on the three-dimensional (3-D) CEM cells' growth. The CEM cells were cultured in the hollow fiber bioreactor (HFB) device. We used (1)H and (19)F magnetic resonance imaging (MRI) to monitor changes in 3-D CEM cell culture. (19)F MRI was used for visualization of the cellular uptake of new fluorine derivatives. Before and after treatment CEM cells profile was investigated with high performance liquid chromatography (HPLC-UV).
Bartusik
Dorota
D
National Research Council Canada, Institute for Biodiagnostics (West), Calgary, Alberta, Canada T2N 4N1. Dorota.Bartusik@gmail.com
Tomanek
Boguslaw
B
Lattová
Erika
E
Perreault
Hélène
H
Tuszynski
Jack
J
Fallone
Gino
G
D019149:Bioreactors; D018929:Cell Culture Techniques; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D003078:Colchicine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D005461:Fluorine / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008279:Magnetic Resonance Imaging; D013056:Spectrophotometry, Ultraviolet; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D005461:Fluorine; D003078:Colchicine