title: A new liquid chromatography-tandem mass spectrometry method for determination of bisoprolol in human plasma samples.
abstract: Liquid chromatography (LC) coupled with mass spectrometry (MS) detection is one of the most powerful analytical tools for organic compound analysis. The advantages of using LC/MS methods over HPLC methods include: selectivity, chromatographic integrity, peak assignment, structural information, and rapid method development. In this paper, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of bisoprolol in human plasma samples, using metoprolol as internal standard and liquid-liquid extraction procedure. The assay has proven to be sensitive, specific and reproducible, suitable to determine the bisoprolol concentration, following a single oral administration of a 10 mg bisoprolol tablet in 22 healthy volunteers, in the bioequivalence study of Bisoprolol 10 mg coated tablets, produced by Antibiotice S.A. versus Concor 10 mg, produced by Merck.
authors.affiliation: Center for Drug Evaluation, Antibiotice S.A., Iasi, Romania. gabrielapeste@yahoo.com
authors.forename: Gabriela
authors.initials: G
authors.lastname: Peste
authors.forename: Nela
authors.initials: N
authors.lastname: Bibire
authors.forename: Mihai
authors.initials: M
authors.lastname: Apostu
authors.forename: Aurel
authors.initials: A
authors.lastname: Vlase
authors.forename: Corneliu
authors.initials: C
authors.lastname: Oniscu
mesh_terms: D017298:Bisoprolol / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013810:Therapeutic Equivalency
chemical_list: D017298:Bisoprolol
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