title: Catalytically incompetent by design.
abstract: Sondermann and colleagues have characterized FimX, a protein with degenerate GGDEF and EAL domains. The study confirms the expected domain folds lacking conserved catalytic residues for c-di-GMP synthesis/degradation, and also defines domain arrangements, providing insight to regulatory mechanisms.
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.forename: Ann M
authors.initials: AM
authors.lastname: Stock
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D018360:Crystallography, X-Ray; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP
references.citation: Structure. 2009 Aug 12;17(8):1104-16
references.pmid: 19679088
references.citation: Nature. 2009 Jun 18;459(7249):1015-8
references.pmid: 19536266
references.citation: Structure. 2007 Aug;15(8):915-27
references.pmid: 17697997
references.citation: J Bacteriol. 2005 Jul;187(14):4774-81
references.pmid: 15995192
references.citation: BMC Microbiol. 2005 Jun 14;5:35
references.pmid: 15955239
references.citation: J Biol Chem. 2005 Sep 2;280(35):30829-37
references.pmid: 15994307
references.citation: Annu Rev Microbiol. 2009;63:133-54
references.pmid: 19575571
references.citation: Nat Rev Microbiol. 2009 Apr;7(4):263-73
references.pmid: 19287449
references.citation: J Biol Chem. 2009 May 8;284(19):13174-84
references.pmid: 19244251
references.citation: J Bacteriol. 2009 Aug;191(15):4722-31
references.pmid: 19376848
references.citation: Genes Dev. 2009 Jan 1;23(1):93-104
references.pmid: 19136627
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3461-6
references.pmid: 19218451