title: Synthesis and biological evaluation of a novel cardiolipin affinity matrix.
abstract: Cardiolipin (1) is a dimeric phospholipid found in the mitochondrial membranes of both plants and animals. In order to understand better its role, we report the preparation of an immobilised analogue (2) using phosphoramidite chemistry; the probe has been used successfully to bind a recombinant protein containing a cardiolipin-binding domain.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: Melloney K
authors.initials: MK
authors.lastname: Johns
authors.forename: Meng-Xin
authors.initials: MX
authors.lastname: Yin
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Conway
authors.forename: Diane E J E
authors.initials: DE
authors.lastname: Robinson
authors.forename: Leon S-M
authors.initials: LS
authors.lastname: Wong
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bamert
authors.forename: Richard E H
authors.initials: RE
authors.lastname: Wettenhall
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Holmes
mesh_terms: D000818:Animals; D002308:Cardiolipins / Q000737:chemistry* / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000737:chemistry; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012685:Sepharose
chemical_list: D002308:Cardiolipins; D009943:Organophosphorus Compounds; D011994:Recombinant Proteins; C434331:phosphoramidite; D012685:Sepharose; C061570:protein kinase N; D011493:Protein Kinase C