title: Proteomic analysis of wild type and arsenite-resistant Leishmania donovani.
abstract: Leishmania donovani, causative organism for visceral leishmaniasis, is responsible for considerable mortality and morbidity worldwide. Generation of drug-resistant variants continue to challenge the chemotherapy, the mainstay to fight the disease. The aim of current study was proteomic profiling of wild type (Ld-Wt) and arsenite-resistant (Ld-As20) L. donovani. Significant differences in protein profiles were observed between Ld-As20 and its parent Ld-Wt strain. Proteomic analysis of 158 spots from Ld-Wt and 144 spots from, Ld-As20 identified 77 and 74 protein entries, respectively, through MALDI-TOF/TOF based mass spectrometry and database search. A shift in the isoelectric point of few proteins was observed both in Ld-Wt and Ld-As20, which raises the possibility of continuous arsenite stress, resulting in the differences in the protein profiles of drug-resistant strain from its parent wild type strain. The comparative proteomic data holds the key for elucidation of the multifactorial and complex drug resistance mechanism, like arsenite resistance, in the parasite.
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER)(2), Sector 67, S.A.S. Nagar, Punjab 160 062, India.
authors.forename: Shruti
authors.initials: S
authors.lastname: Sharma
authors.forename: Gaganmeet
authors.initials: G
authors.lastname: Singh
authors.forename: Hemantkumar D
authors.initials: HD
authors.lastname: Chavan
authors.forename: Chinmoy S
authors.initials: CS
authors.lastname: Dey
mesh_terms: D000818:Animals; D018053:Arsenites / Q000494:pharmacology*; D004351:Drug Resistance; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007893:Leishmania donovani / Q000737:chemistry* / Q000187:drug effects; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism; D017670:Sodium Compounds / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018053:Arsenites; D004791:Enzyme Inhibitors; D015800:Protozoan Proteins; D017670:Sodium Compounds; C017947:sodium arsenite