title: Influence of a model human defensive peroxidase system on oral streptococcal antagonism.
abstract: Streptococcus is a dominant genus in the human oral cavity, making up about 20 % of the more than 800 species of bacteria that have been identified, and about 80 % of the early biofilm colonizers. Oral streptococci include both health-compatible (e.g. Streptococcus gordonii and Streptococcus sanguinis) and pathogenic strains (e.g. the cariogenic Streptococcus mutans). Because the streptococci have similar metabolic requirements, they have developed defence strategies that lead to antagonism (also known as bacterial interference). S. mutans expresses bacteriocins that are cytotoxic toward S. gordonii and S. sanguinis, whereas S. gordonii and S. sanguinis differentially produce H(2)O(2) (under aerobic growth conditions), which is relatively toxic toward S. mutans. Superimposed on the inter-bacterial combat are the effects of the host defensive mechanisms. We report here on the multifarious effects of bovine lactoperoxidase (bLPO) on the antagonism between S. gordonii and S. sanguinis versus S. mutans. Some of the effects are apparently counterproductive with respect to maintaining a health-compatible population of streptococci. For example, the bLPO system (comprised of bLPO+SCN(-)+H(2)O(2)) destroys H(2)O(2), thereby abolishing the ability of S. gordonii and S. sanguinis to inhibit the growth of S. mutans. Furthermore, bLPO protein (with or without its substrate) inhibits bacterial growth in a biofilm assay, but sucrose negates the inhibitory effects of the bLPO protein, thereby facilitating adherence of S. mutans in lieu of S. gordonii and S. sanguinis. Our findings may be relevant to environmental pressures that select early supragingival colonizers.
authors.affiliation: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Ashby
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Jens
authors.initials: J
authors.lastname: Kreth
authors.affiliation: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Muthu
authors.initials: M
authors.lastname: Soundarajan
authors.affiliation: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Laure Sita
authors.initials: LS
authors.lastname: Sivuilu
mesh_terms: D000818:Animals; D000898:Antibiosis; D018441:Biofilms / Q000254:growth & development; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D006861:Hydrogen Peroxide / Q000378:metabolism; D007784:Lactoperoxidase / Q000378:metabolism*; D009055:Mouth / Q000382:microbiology; D054773:Streptococcus gordonii / Q000254:growth & development*; D013295:Streptococcus mutans / Q000254:growth & development*; D013298:Streptococcus sanguis / Q000254:growth & development*; D013861:Thiocyanates / Q000378:metabolism
chemical_list: D013861:Thiocyanates; C014607:hypothiocyanite ion; D006861:Hydrogen Peroxide; D007784:Lactoperoxidase; D002374:Catalase
references.citation: Infect Immun. 1983 Feb;39(2):767-78
references.pmid: 6832819
references.citation: Biochim Biophys Acta. 1986 Apr 22;870(3):377-84
references.pmid: 3697357
references.citation: J Clin Microbiol. 2005 Nov;43(11):5721-32
references.pmid: 16272510
references.citation: Arch Biochem Biophys. 2006 Jan 15;445(2):261-8
references.pmid: 16111647
references.citation: Periodontol 2000. 2006;42:80-7
references.pmid: 16930307
references.citation: Caries Res. 2001 Jul-Aug;35(4):258-64
references.pmid: 11423721
references.citation: J Dent Res. 2008 Oct;87(10):900-14
references.pmid: 18809743
references.citation: Odontology. 2006 Sep;94(1):1-9
references.pmid: 16998612
references.citation: J Periodontol. 1995 Sep;66(9):766-74
references.pmid: 7500242
references.citation: J Clin Periodontol. 2005;32 Suppl 6:7-15
references.pmid: 16128825
references.citation: Biochim Biophys Acta. 1981 Apr 14;658(2):238-47
references.pmid: 7248302
references.citation: Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14547-52
references.pmid: 10588742
references.citation: J Bacteriol. 2001 Jun;183(12):3770-83
references.pmid: 11371542
references.citation: J Dent Res. 1990 Feb;69 Spec No:653-9; discussion 682-3
references.pmid: 2179326
references.citation: Nature. 2007 Oct 18;449(7164):811-8
references.pmid: 17943117
references.citation: Nat Rev Microbiol. 2008 Sep;6(9):693-9
references.pmid: 18587409
references.citation: Anal Biochem. 2003 Jan 15;312(2):224-7
references.pmid: 12531209
references.citation: J Bacteriol. 2007 Apr;189(8):3166-75
references.pmid: 17277061
references.citation: Biochemistry. 1988 Jan 12;27(1):233-9
references.pmid: 3349028
references.citation: FEMS Microbiol Ecol. 2008 Dec;66(3):637-44
references.pmid: 18785881
references.citation: Arch Biochem Biophys. 1959 May;82(1):70-7
references.pmid: 13650640
references.citation: J Gen Microbiol. 1963 Apr;31:125-33
references.pmid: 13941150
references.citation: J Dent Res. 2001 May;80(5):1436-40
references.pmid: 11437215
references.citation: J Clin Microbiol. 1985 Dec;22(6):996-1006
references.pmid: 3905855
references.citation: Microbes Infect. 2000 Nov;2(13):1599-607
references.pmid: 11113379
references.citation: Biochemistry. 1998 Jun 23;37(25):8965-72
references.pmid: 9636038
references.citation: J Dent Res. 1987 Feb;66(2):616-8
references.pmid: 3476578
references.citation: J Dairy Res. 1986 Nov;53(4):507-14
references.pmid: 3794018
references.citation: J Contemp Dent Pract. 2000 Aug 15;1(3):18-29
references.pmid: 12167880
references.citation: Appl Environ Microbiol. 2006 Oct;72(10):6734-42
references.pmid: 17021225
references.citation: Arch Oral Biol. 1975 Oct;20(10):641-8
references.pmid: 1059386
references.citation: Anal Biochem. 1979 Apr 1;94(1):75-81
references.pmid: 37780
references.citation: J Clin Microbiol. 2008 Apr;46(4):1407-17
references.pmid: 18216213
references.citation: Talanta. 2000 May 5;51(6):1049-58
references.pmid: 18967936
references.citation: Caries Res. 1993;27(1):26-30
references.pmid: 8448770
references.citation: J Anat. 1978 Sep;127(Pt 1):181-91
references.pmid: 701194
references.citation: J Clin Microbiol. 2008 Jun;46(6):2015-21
references.pmid: 18385433
references.citation: J Dent Res. 2005 Dec;84(12):1172-7
references.pmid: 16304449
references.citation: Infect Immun. 1982 Jan;35(1):20-4
references.pmid: 7033135
references.citation: J Bacteriol. 2008 Jul;190(13):4632-40
references.pmid: 18441055
references.citation: Eur J Biochem. 1977 Oct 17;80(1):209-14
references.pmid: 562752
references.citation: Oral Microbiol Immunol. 2008 Jun;23(3):213-9
references.pmid: 18402607
references.citation: J Dent Res. 1987 Feb;66(2):486-91
references.pmid: 3040825
references.citation: Periodontol 2000. 2005;38:135-87
references.pmid: 15853940
references.citation: Infect Immun. 1980 Sep;29(3):1190-2
references.pmid: 7429633
references.citation: FEMS Microbiol Lett. 2000 Dec 1;193(1):1-6
references.pmid: 11094270
references.citation: Caries Res. 2007;41(5):413-22
references.pmid: 17713343
references.citation: J Dent Res. 1977 Dec;56(12):1603-7
references.pmid: 277482
references.citation: Infect Immun. 1994 Feb;62(2):529-35
references.pmid: 8300211
references.citation: J Clin Periodontol. 2001 Jul;28(7):697-700
references.pmid: 11422593
references.citation: Appl Environ Microbiol. 1979 Nov;38(5):821-6
references.pmid: 396883
references.citation: J Appl Microbiol. 2001 Apr;90(4):489-93
references.pmid: 11309058
references.citation: Appl Environ Microbiol. 2001 Jan;67(1):15-21
references.pmid: 11133423
references.citation: Infect Immun. 1983 Apr;40(1):70-80
references.pmid: 6832837
references.citation: J Bacteriol. 1983 Jun;154(3):1236-44
references.pmid: 6304008
references.citation: Adv Dent Res. 1997 Apr;11(1):110-26
references.pmid: 9524448
references.citation: J Gen Microbiol. 1966 Apr;43(1):31-43
references.pmid: 5333458
references.citation: J Dent Res. 1977 Dec;56(12):1608-13
references.pmid: 277483
references.citation: J Dairy Sci. 1985 May;68(5):1095-102
references.pmid: 3916277
references.citation: Dent Clin North Am. 1999 Oct;43(4):599-614, v-vi
references.pmid: 10553246
references.citation: J Clin Microbiol. 2002 Mar;40(3):1001-9
references.pmid: 11880430
references.citation: Infect Immun. 1977 Jul;17(1):112-6
references.pmid: 885609
references.citation: Biochemistry. 1981 May 26;20(11):3273-80
references.pmid: 7248282
references.citation: Arch Oral Biol. 1985;30(7):519-23
references.pmid: 3864401
references.citation: J Dent Res. 1981 Apr;60(4):831-7
references.pmid: 6259229
references.citation: J Oral Pathol. 1987 Sep;16(8):412-6
references.pmid: 2826736
references.citation: Arch Oral Biol. 1995 Aug;40(8):753-63
references.pmid: 7487577