Detection and molecular characterization of Giardia isolated  from recreational lake water in Malaysia.
Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8% of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.
Lim
Yvonne A L
YA
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. limailian@um.edu.my
Ramasame
Sharmila D
SD
Mahdy
Mohammed A K
MA
Sulaiman
Wan Yusoff W
WY
Smith
Huw V
HV
D000818:Animals; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000469:parasitology*; D020459:Genes, rRNA; D005872:Giardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008296:Malaysia; D008969:Molecular Sequence Data; D010274:Parasitology / Q000379:methods; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
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