Variations of water uptake, lipid consumption, and dynamics during the germination of Sesamum indicum seed: a nuclear magnetic resonance spectroscopic investigation.
Germination in sesame seeds (Sesamum indicum L.) in water and in indole-3-acetic acid (IAA) solution is investigated with magic-angle-spinning (MAS) solid state nuclear magnetic resonance (NMR) spectroscopy, supplemented by liquid state NMR spectroscopy. The spectra show good resolution and can be assigned with sufficient confidence. The characteristic spectral peaks and relaxation rates were monitored during the entire course of germination for better understanding of the biophysical and biochemical mechanisms involved in the triphasic water uptake of the seed. A highly positive correlation is found between water uptake and lipid consumption during germination. No significant variation is observed in the relaxation times for the lipid protons during the first two stages of triphasic water uptake, while evident differences are observed for water proton relaxation rates in all stages. Although the total amount of water uptake is largely not changed as a result of IAA, the addition of IAA in seed-germination medium has shown some prominent effects on the germination process, e.g, it suppresses lipid consumption and water mobility, and it reduces the longitudinal and transverse relaxation times of lipid protons and causes a more scattered range for these parameters.
Sarkar
Bimal Kumar
BK
Department of Chemistry & Center for Nanoscience and Nanotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan 80424, Republic of China. ding@faculty.nsysu.edu.tw
Yang
Wei-Yuan
WY
Wu
Zhen
Z
Tang
Huiru
H
Ding
Shangwu
S
D005227:Fatty Acids / Q000032:analysis; D018525:Germination / Q000187:drug effects / Q000502:physiology*; D007210:Indoleacetic Acids / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D010937:Plant Growth Regulators / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D012715:Sesame Oil / Q000032:analysis / Q000378:metabolism; D031685:Sesamum / Q000196:embryology*; D014867:Water / Q000378:metabolism*
D005227:Fatty Acids; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D014867:Water; C030737:indoleacetic acid; D012715:Sesame Oil