Combination treatment with resveratrol and sulforaphane induces apoptosis in human U251 glioma cells.
Resveratrol is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Sulforaphane belongs to the family of isothiocyanates and is highly enriched in cruciferous vegetables. Our previous study showed that resveratrol, when used at high concentrations, inhibited cell proliferation, caused the cell cycle arrest and induced apoptotic cell death in glioma cells. In the current study, we tested the effect of combination treatment with resveratrol and sulforaphane, when both were used at low concentrations, on cell proliferation, migration and death in human U251 glioma cells. Our study shows that combination treatment with resveratrol and sulforaphane inhibits cell proliferation and migration, reduces cell viability, induces lactate dehydrogenase release, decreases pro-survival Akt phosphorylation and increases caspase-3 activation. The use of combination of bioactive food components, such as resveratrol and sulforaphane, may be a viable approach for the treatment of glioma.
Jiang
Hao
H
Department of Neurology, Henry Ford Hospital, Detroit, MI 48202, USA. hjiang1@hfhs.org
Shang
Xia
X
Wu
Hongtao
H
Huang
Grace
G
Wang
Yiyang
Y
Al-Holou
Shaza
S
Gautam
Subhash C
SC
Chopp
Michael
M
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005910:Glioma / Q000473:pathology*; D006801:Humans; D017879:Isothiocyanates; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology*
D017879:Isothiocyanates; D013267:Stilbenes; D013454:Sulfoxides; D013861:Thiocyanates; C016766:sulforaphane; D000077185:Resveratrol
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