title: Molecular and serologic markers of acute dengue infection in naive and flavivirus-vaccinated travelers.
abstract: A total of 520 European travelers with suspected dengue fever were examined, and acute dengue virus infection was confirmed in 127 of them. Molecular and serological tests for dengue diagnosis, according to their usefulness in the different stages of the disease, were performed. The accuracy of the IgM/IgG ratio and the IgG avidity index was confirmed during the early phase of the illness to discriminate the serologic status (primary versus secondary immune response) of patients who were either naive or previously vaccinated against other flaviviruses. Virologic markers of secondary infection were detected in 11.8% of nonvaccinated infected patients and in 92.6% of yellow fever vaccinated patients. The proper use of these simple methodologies could help in the identification of patients affected with secondary infection, who may be at a higher risk of developing a hemorrhagic disorder.
authors.affiliation: Diagnostic Microbiology Service, National Center for Microbiology, Instituto de Salud Carlos III, 28220 Madrid, Spain. domingo-carrascoc@rki.de
authors.forename: Cristina
authors.initials: C
authors.lastname: Domingo
authors.forename: Fernando
authors.initials: F
authors.lastname: de Ory
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanz
authors.forename: Noelia
authors.initials: N
authors.lastname: Reyes
authors.forename: Joaquim
authors.initials: J
authors.lastname: Gascón
authors.forename: Ole
authors.initials: O
authors.lastname: Wichmann
authors.forename: Sabino
authors.initials: S
authors.lastname: Puente
authors.forename: Mirjam
authors.initials: M
authors.lastname: Schunk
authors.forename: Rogelio
authors.initials: R
authors.lastname: López-Vélez
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ruiz
authors.forename: Antonio
authors.initials: A
authors.lastname: Tenorio
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D000915:Antibody Affinity; D015415:Biomarkers; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000235:genetics* / Q000276:immunology*; D005416:Flavivirus / Q000276:immunology*; D005819:Genetic Markers; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D014195:Travel; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D015415:Biomarkers; D005819:Genetic Markers; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014765:Viral Vaccines