title: [Preparation and identification of a polyclonal antibody against novel cysteinyl leukotriene receptor GPR17].
abstract: OBJECTIVE
  To prepare and identify a polyclonal antibody (pAb) against GPR17, a novel cysteinyl leukotriene receptor.
  
  
  METHODS
  Rabbits were immunized with KLH-coupled GPR17 peptide to prepare the pAb. The titer of the pAb in rabbit plasma was detected by indirect ELISA, and the specificity of the pAb was tested by antigen blockade. GPR17 tissue distribution was detected by Western blot with the pAb.
  
  
  RESULTS
  The pAb showed a titer as high as 1:16 364,and was not cross-reacted with the antigens of CysLT(1) and CysLT(2) receptors. A higher expression of GPR17 in the rat brain and heart was detected using the newly prepared pAb. The molecular weigh of GPR17 protein was about 43 kD.
  
  
  CONCLUSIONS
  The prepared GPR17 pAb has high sensitivity and specificity,and can be used in Western blot for detecting GPR17.
authors.affiliation: Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: QI
authors.forename: Yun-bi
authors.initials: YB
authors.lastname: LU
authors.forename: Wen-zhen
authors.initials: WZ
authors.lastname: SHI
authors.forename: Chun-zhen
authors.initials: CZ
authors.lastname: ZHAO
authors.forename: Yong-mei
authors.initials: YM
authors.lastname: ZHANG
authors.forename: Li-ping
authors.initials: LP
authors.lastname: CHEN
authors.forename: Li-hui
authors.initials: LH
authors.lastname: ZHANG
authors.forename: San-hua
authors.initials: SH
authors.lastname: FANG
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: BAO
authors.forename: Jian-gen
authors.initials: JG
authors.lastname: SHEN
authors.forename: Er-qing
authors.initials: EQ
authors.lastname: WEI
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011817:Rabbits; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000276:immunology*; D018077:Receptors, Leukotriene / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C121670:GPR17 protein, human; D043562:Receptors, G-Protein-Coupled; D018077:Receptors, Leukotriene; C083169:leukotriene D4 receptor