The development of genipin-crosslinked poly(caprolactone) (PCL)/gelatin nanofibers for tissue engineering applications.
Composite nanofibers of poly(caprolactone) (PCL) and gelatin crosslinked with genipin are prepared. The contact angles and mechanical properties of crosslinked PCL-gelatin nanofibers decrease as the gelatin content increases. The proliferation of myoblasts is higher in the crosslinked PCL-gelatin nanofibers than in the PCL nanofibers, and the formation of myotubes is only observed on the crosslinked PCL-gelatin nanofibers. The expression level of myogenin, myosin heavy chain, and troponin T genes is increased as the gelatin content is increased. The results suggest that PCL-gelatin nanofibers crosslinked with genipin can be used as a substrate to modulate proliferation and differentiation of myoblasts, presenting potential applications in muscle tissue engineering.
Kim
Min Sup
MS
Department of Biomedical Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Korea.
Jun
Indong
I
Shin
Young Min
YM
Jang
Wonhee
W
Kim
Sun I
SI
Shin
Heungsoo
H
D015153:Blotting, Western; D003432:Cross-Linking Reagents / Q000737:chemistry*; D017931:DNA Primers / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005780:Gelatin / Q000138:chemical synthesis* / Q000737:chemistry; D057889:Iridoid Glycosides; D039823:Iridoids / Q000737:chemistry*; D024510:Muscle Development / Q000502:physiology*; D018008:Myogenin / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D020107:Troponin T / Q000378:metabolism
D003432:Cross-Linking Reagents; D017931:DNA Primers; D057889:Iridoid Glycosides; D039823:Iridoids; D018008:Myogenin; D011091:Polyesters; D020107:Troponin T; C016240:polycaprolactone; D005780:Gelatin; C007834:genipin; D018995:Myosin Heavy Chains