title: A MOFRL family glycerate kinase from the thermophilic crenarchaeon, Sulfolobus tokodaii, with unique enzymatic properties.
abstract: A glycerate kinase gene (ST2037) from the hyperthermophilic crenarchaeon Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The purified homodimeric protein (45 kDa) specifically catalyzed the formation of 2-phosphoglycerate with D-glycerate as substrate. The thermostable enzyme displayed maximum activity (over 20 min) at 90 degrees C and pH 4.5. The maximal activity was in the presence of Co(2+). The MOFRL family glycerate kinase used AMP as phosphate donor with maximal activity towards GTP. These characteristics of the enzyme suggested its potential in the catalytic production of 2-phosphoglycerate.
authors.affiliation: College of Food and Bioengineering, Shandong Institute of Light Industry, Jinan, 250353, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Tianming
authors.initials: T
authors.lastname: Liu
authors.forename: Ye
authors.initials: Y
authors.lastname: Hong
authors.forename: Yulong
authors.initials: Y
authors.lastname: Shen
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Ni
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D003001:Cloning, Molecular; D003035:Cobalt / Q000494:pharmacology; D003067:Coenzymes / Q000494:pharmacology; D019281:Dimerization; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005988:Glyceric Acids / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016944:Sulfolobus / Q000201:enzymology* / Q000235:genetics*; D013696:Temperature
chemical_list: D003067:Coenzymes; D005988:Glyceric Acids; D011994:Recombinant Proteins; C008885:2-phosphoglycerate; D003035:Cobalt; D000249:Adenosine Monophosphate; C042971:glyceric acid; D006160:Guanosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C020670:glycerate kinase