Pitfalls in the use of arachidonic acid oxidation products to assign lipoxygenase activity in cancer cells.
Arachidonic acid (AA) reaction with cyclooxygenase (COX) and lipoxygenases (LOX) yield eicosanoids that can mediate prostate cancer proliferation and enhance both tumour vascularization and metastasis. Increasingly measurement of eicosanoids with liquid chromatography is employed to implicate LOX activity in different biological systems and in particular link LOX activity to the progression of cancer in experimental models. This study demonstrates that simply identifying patterns of eicosanoid regio-isomerism is insufficient to designate LOX activity in prostate cancer cells and the analysis must include complete stereochemical assignment of the various isomers in order to validate the assignment of LOX activity.
Niknami
Marzieh
M
Cancer Biology Group, Department of Endocrinology and Sydney Cancer Centre, Central Clinical School, The University of Sydney, NSW, Australia.
Dong
Qihan
Q
Witting
Paul K
PK
D016718:Arachidonic Acid / Q000378:metabolism*; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D015777:Eicosanoids / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D013237:Stereoisomerism
D015777:Eicosanoids; D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases