title: Morphological and proliferative characteristics of vero and MDCK cells during culturing in nutrient media on the basis of hydrolysates of plant proteins.
abstract: Morphological and proliferative characteristics of cultured Vero and MDCK cells were compared after growth in nutrient media of the basis of enzymatic hydrolysates of rice flour proteins and soybean flour proteins or control media (DMEM and Axcevir-MDCK). These media had a strong stimulatory effect on the growth of cultured Vero cells (addition of 3% fetal bovine serum, Gibco), but did not modulate the morphology of this culture. Culturing of MDCK cells in nutrient media on the basis of enzymatic hydrolysates of rice and soybean flour proteins with low content of fetal bovine serum (2%, Gibco) was accompanied by insignificant changes in the index of proliferation and morphological characteristics of cultured cells.
authors.affiliation: Federal State Institution of Science, State Research Center of Virology and Biotechnology Vector, Russian Consumer Supervision, Novosibirsk oblast, Kol'tsovo.
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: E I
authors.initials: EI
authors.lastname: Ryabchikova
authors.forename: G P
authors.initials: GP
authors.lastname: Troshkova
authors.forename: T N
authors.initials: TN
authors.lastname: Getmanova
authors.forename: T P
authors.initials: TP
authors.lastname: Sumkina
authors.forename: L N
authors.initials: LN
authors.lastname: Shishkina
authors.forename: A N
authors.initials: AN
authors.lastname: Sergeev
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D002522:Chlorocebus aethiops; D003470:Culture Media; D004285:Dogs; D005260:Female; D046529:Microscopy, Electron, Transmission; D008858:Microscopy, Phase-Contrast; D012275:Oryza; D011492:Protein Hydrolysates; D013025:Glycine max; D014709:Vero Cells
chemical_list: D003470:Culture Media; D011492:Protein Hydrolysates