Simultaneous detection of DNA from 10 food allergens by ligation-dependent probe amplification.
The simultaneous detection of DNA from different allergenic food ingredients by a ligation-dependent probe amplification (LPA) system is described. The approach allows detection of several targets in a one-tube assay. Synthetic oligonucleotides were designed to detect DNA from peanuts, cashews, pecans, pistachios, hazelnuts, sesame seeds, macadamia nuts, almonds, walnuts and brazil nuts. The specificity of the system was tested with DNA from more than 50 plant and animal species. The sensitivity of the method was suitable to detect allergenic ingredients in the low mg kg(-1) range. The limit of detection (LOD) for single allergens in different food matrices was 5 mg kg(-1). The novel analytical strategy represents a useful tool for the surveillance of established legislation on food allergens within the European Union.
Ehlert
Alexandra
A
Department of General Food Technology, Technische Universität München, Freising-Weihenstephan D-85350, Germany.
Demmel
Anja
A
Hupfer
Christine
C
Busch
Ulrich
U
Engel
Karl-Heinz
KH
D000485:Allergens / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D016133:Polymerase Chain Reaction / Q000379:methods*
D000485:Allergens; D017931:DNA Primers; D015342:DNA Probes; D004247:DNA