title: CP250, a novel acidic coiled-coil protein of the Dictyostelium centrosome, affects growth, chemotaxis, and the nuclear envelope.
abstract: The Dictyostelium centrosome is a nucleus associated body consisting of a box-shaped core surrounded by the corona, an amorphous matrix functionally equivalent to the pericentriolar material of animal centrosomes which is responsible for the nucleation and anchoring of microtubules. Here we describe CP250 a component of the corona, an acidic coiled coil protein that is present at the centrosome throughout interphase while disappearing during prophase and reappearing at the end of late telophase. Amino acids 756-1148 of the 2110 amino acids are sufficient for centrosomal targeting and cell cycle-dependent centrosome association. Mutant cells lacking CP250 are smaller in size, growth on bacteria is delayed, chemotaxis is altered, and development is affected, which, in general, are defects observed in cytoskeletal mutants. Furthermore, loss of CP250 affected the nuclear envelope and led to reduced amounts and altered distribution of Sun-1, a conserved nuclear envelope protein that connects the centrosome to chromatin.
authors.affiliation: Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Köln, Germany.
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Blau-Wasser
authors.forename: Ursula
authors.initials: U
authors.lastname: Euteneuer
authors.forename: Huajiang
authors.initials: H
authors.lastname: Xiong
authors.forename: Berthold
authors.initials: B
authors.lastname: Gassen
authors.forename: Michael
authors.initials: M
authors.lastname: Schleicher
authors.forename: Angelika A
authors.initials: AA
authors.lastname: Noegel
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D048430:Cell Shape; D018385:Centrosome / Q000187:drug effects / Q000648:ultrastructure; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D004023:Dictyostelium / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D055786:Gene Knockout Techniques; D007399:Interphase; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008938:Mitosis; D009685:Nuclear Envelope / Q000737:chemistry*; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000502:physiology; D050257:Tubulin Modulators / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
chemical_list: D008869:Microtubule-Associated Proteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D050257:Tubulin Modulators; D000242:Cyclic AMP
references.citation: Mol Biol Cell. 1999 Jan;10(1):151-60
references.pmid: 9880333
references.citation: Cell Mol Life Sci. 2009 May;66(9):1518-33
references.pmid: 19125221
references.citation: J Cell Sci. 2007 Jul 1;120(Pt 13):2139-42
references.pmid: 17591686
references.citation: EMBO J. 1999 Dec 1;18(23):6786-92
references.pmid: 10581251
references.citation: Mol Biol Cell. 2005 Jun;16(6):2759-71
references.pmid: 15800059
references.citation: Traffic. 2008 May;9(5):708-24
references.pmid: 18266910
references.citation: Nat Rev Mol Cell Biol. 2008 Nov;9(11):860-73
references.pmid: 18946475
references.citation: J Cell Biol. 1982 Jun;93(3):576-82
references.pmid: 6811596
references.citation: Nature. 1977 Nov 3;270(5632):80-2
references.pmid: 927525
references.citation: Nature. 2008 Dec 4;456(7222):667-70
references.pmid: 18997772
references.citation: J Cell Sci. 2000 May;113 ( Pt 10):1747-58
references.pmid: 10769206
references.citation: Dev Cell. 2007 Jun;12(6):863-72
references.pmid: 17543860
references.citation: J Cell Biol. 1994 Mar;124(5):783-93
references.pmid: 8120099
references.citation: Eur J Cell Biol. 2002 Apr;81(4):175-84
references.pmid: 12018385
references.citation: Proc Natl Acad Sci U S A. 2007 May 1;104(18):7426-31
references.pmid: 17452644
references.citation: Cell. 2003 Dec 26;115(7):825-36
references.pmid: 14697201
references.citation: Z Allg Mikrobiol. 1982;22(2):127-31
references.pmid: 7201213
references.citation: Genes Dev. 2008 Aug 15;22(16):2278-90
references.pmid: 18708585
references.citation: Eur J Cell Biol. 1979 Jun;19(2):109-15
references.pmid: 467454
references.citation: Methods Cell Biol. 2001;67:337-57
references.pmid: 11550479
references.citation: EMBO J. 1991 May;10(5):1245-54
references.pmid: 2022189
references.citation: J Cell Biol. 2008 Feb 25;180(4):681-9
references.pmid: 18283114
references.citation: Trends Mol Med. 2006 Sep;12(9):415-24
references.pmid: 16890490
references.citation: J Cell Biol. 1998 Aug 10;142(3):735-50
references.pmid: 9700162
references.citation: Nucleic Acids Res. 2004 Oct 26;32(19):e143
references.pmid: 15507682
references.citation: Mol Biol Cell. 2002 Jul;13(7):2301-10
references.pmid: 12134070
references.citation: J Cell Sci. 2005 Aug 1;118(Pt 15):3419-30
references.pmid: 16079285
references.citation: Dev Cell. 2007 Aug;13(2):190-202
references.pmid: 17681131
references.citation: Mol Biol Cell. 2004 Feb;15(2):934-45
references.pmid: 14595119
references.citation: J Cell Biol. 1995 Mar;128(5):819-35
references.pmid: 7876307
references.citation: J Cell Sci. 2009 Mar 1;122(Pt 5):577-86
references.pmid: 19225124
references.citation: Eur J Cell Biol. 2001 Oct;80(10):621-30
references.pmid: 11713866
references.citation: Mol Biol Cell. 2003 Jun;14(6):2436-46
references.pmid: 12808041
references.citation: J Cell Sci. 1996 Dec;109 ( Pt 13):3103-12
references.pmid: 9004044
references.citation: Cell Motil Cytoskeleton. 1998;41(3):225-46
references.pmid: 9829777
references.citation: Dev Cell. 2004 Dec;7(6):815-29
references.pmid: 15572125
references.citation: Mol Biol Cell. 1996 Feb;7(2):261-72
references.pmid: 8688557
references.citation: Genes Dev. 1993 Apr;7(4):555-69
references.pmid: 8384581
references.citation: Genes Dev. 2008 Jan 1;22(1):91-105
references.pmid: 18086858
references.citation: J Cell Sci. 2002 May 1;115(Pt 9):1919-29
references.pmid: 11956323
references.citation: Mol Biosyst. 2007 Sep;3(9):583-9
references.pmid: 17700857
references.citation: J Cell Biol. 2006 Aug 28;174(5):665-75
references.pmid: 16923827
references.citation: DNA Cell Biol. 2006 Oct;25(10):554-62
references.pmid: 17132086
references.citation: J Cell Sci. 2008 Jun 1;121(11):1887-98
references.pmid: 18477613
references.citation: Cell Mol Life Sci. 2007 Oct;64(19-20):2702-15
references.pmid: 17805484
references.citation: Mol Genet Genomics. 2004 Jan;270(6):449-61
references.pmid: 14655046
references.citation: J Cell Sci. 1999 Oct;112 ( Pt 19):3195-203
references.pmid: 10504325
references.citation: Mol Cell Biol. 2000 Oct;20(20):7813-25
references.pmid: 11003675
references.citation: J Cell Biol. 1989 Aug;109(2):607-18
references.pmid: 2668299
references.citation: J Cell Sci. 1998 Feb;111 ( Pt 3):405-12
references.pmid: 9427688
references.citation: J Proteome Res. 2006 Mar;5(3):589-98
references.pmid: 16512674
references.citation: J Cell Biol. 1984 Jul;99(1 Pt 1):287-95
references.pmid: 6203918
references.citation: Proc Natl Acad Sci U S A. 1998 May 26;95(11):5857-64
references.pmid: 9600884
references.citation: J Cell Sci. 2004 Oct 1;117(Pt 21):5013-22
references.pmid: 15383615
references.citation: Biol Cell. 1999 Jul;91(6):471-7
references.pmid: 10519007
references.citation: J Cell Sci. 2005 Jan 15;118(Pt 2):447-59
references.pmid: 15654021
references.citation: Annu Rev Cell Dev Biol. 2005;21:411-34
references.pmid: 16212501
references.citation: EMBO J. 2002 Oct 15;21(20):5331-42
references.pmid: 12374734