Effects of ascorbic acid on calcium signaling in tumor cells.
Effects of ascorbic acid on calcium homeostasis of human laryngeal carcinoma cells were studied. Intracellular concentration of free calcium and intracellular pH were measured by fluorescent analysis. Ascorbic acid in concentrations of 3-10 mM caused pH drop and sharply increased concentrations of free Ca ions in HEp-2 cells. Intracellular concentration of free Ca ions resulted from Ca ion release from the thapsigargin-sensitive Ca depots.
Martinovich
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Department of Biophysics, Byelorussian State University, Minsk, Byelorussia. martinovichgg@bsu.by
Martinovich
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Cherenkevich
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D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D002277:Carcinoma / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects; D005453:Fluorescence; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology; D013997:Time Factors
D000975:Antioxidants; D004791:Enzyme Inhibitors; D016877:Oxidants; D019284:Thapsigargin; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D002118:Calcium