title: Significance of deep section in small gastric biopsies.
abstract: OBJECTIVE
  Chronic gastritis is defined as the presence of chronic mucosal inflammatory changes leading eventually to mucosal atrophy and epithelial metaplasia. This condition constitutes a background for dysplasia and thereby carcinoma. Detection of exact histopathology of inflammatory process is necessary in biopsy specimen. We designed the current study to determine the value of taking more sections in small gastric biopsies for better histopathologic evaluation.
  
  
  METHODS
  Gastric biopsy specimen of children who suffered from gastrointestinal (GI) symptoms was sent in 10% formalin to our laboratory. After routine processing, three slides with several sections on them were taken from the specimen: t0 he first was named the superficial section, the second was stained by Giemsa and the third was named deep section (further sections after this slide will diminish in size). The slides were not taken exactly consecutively but several sections were discarded between them. The purpose of this study is to compare the superficial and deep sections for detection of inflammatory processes.
  
  
  RESULTS
  In 1062 specimens the results of superficial section and deep section were the same (87.1%) and in 158 specimens the results were different. In 88 (7.2%) specimens deep section was diagnostic. The difference was seen usually as normal tissue in superficial sections but presence of lymphoid follicle in deep sections. The difference between superficial and deep sections was statistically significant. Although obtaining more sections will put an economic burden on the laboratory, we propose that in small gastric biopsies, it is helpful in better evaluation of histopathological changes.
authors.affiliation: Markaze Tebbi Koodakan (Children Hospital), Tehran University of Medical Sciences, Tehran, Iran. fmahjoub@sina.tums.ac.ir
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mahjoub
authors.forename: Morteza
authors.initials: M
authors.lastname: Khademalhosseini
authors.forename: Maryam
authors.initials: M
authors.lastname: Monajemzadeh
authors.forename: Mehri Najafi
authors.initials: MN
authors.lastname: Sani
mesh_terms: D001706:Biopsy / Q000379:methods*; D002648:Child; D005756:Gastritis / Q000175:diagnosis*; D006801:Humans; D010338:Pathology, Clinical / Q000379:methods*; D012680:Sensitivity and Specificity; D013270:Stomach / Q000473:pathology*