An electrospray ionization mass spectrometric study of iron binding to amyloid-beta peptides.
Iron and other metal ions appear to play an important role in protein aggregation and are therefore likely to provide a link between protein aggregation and oxidative damage. This work reports on iron binding to amyloid- beta peptide (Abeta1-40), which affords a very specific electrospray ionization mass spectrometric (MS) spectrum. Both MS and MS/MS study confirmed that amyloid-beta peptide displays a high affinity toward iron(III) ions, producing multi charged molecular ions and peptide aggregates. Finally, the circular dichroism spectra indicate an unexpected modification of Abeta1-40 peptide conformation upon iron binding.
Drochioiu
Gabi
G
Al. I. Cuza University of Iasi, Faculty of Chemistry, 11 Carol I, Iasi-700506, Romania. gabidr@uaic.ro
D016229:Amyloid beta-Peptides / Q000378:metabolism*; D002942:Circular Dichroism; D005290:Ferric Compounds / Q000378:metabolism*; D055503:Protein Multimerization / Q000187:drug effects; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D016229:Amyloid beta-Peptides; D005290:Ferric Compounds