Analysis of nonderivatized steroids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using C70 fullerene as matrix.
Neutral steroid hormones are currently analyzed by gas or liquid chromatography/mass spectrometry based methods. Most of the steroid compounds, however, lack volatility and do not contain polar groups, which results in inadequate chromatographic behavior and low ionization efficiency. Derivatization of the steroids to form more volatile, thermostable, and charged products solves this difficulty, but the derivatization of compounds with unknown chemical moieties is not an easy task. In this study, a rapid, high-throughput, sensitive matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method is described using C(70) fullerene as a matrix compound. The application of the method is demonstrated for five general sex steroids and for synthetic steroid compounds in both negative and positive ionization modes.
Montsko
Gergely
G
Institute of Biochemistry and Medical Chemistry, University of Pecs, 12 Szigeti str, 7624, Pecs, Hungary.
Vaczy
Alexandra
A
Maasz
Gabor
G
Mernyak
Erzsebet
E
Frank
Eva
E
Bay
Csaba
C
Kadar
Zalan
Z
Ohmacht
Robert
R
Wolfling
Janos
J
Mark
Laszlo
L
D000328:Adult; D004958:Estradiol / Q000652:urine; D004964:Estriol / Q000652:urine; D005260:Female; D037741:Fullerenes; D006801:Humans; D011247:Pregnancy; D011374:Progesterone / Q000652:urine; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*; D013256:Steroids / Q000032:analysis* / Q000652:urine*
D037741:Fullerenes; D013256:Steroids; C069836:fullerene C70; D011374:Progesterone; D004958:Estradiol; D004964:Estriol