title: The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA.
abstract: There are three stages of transcribing DNA into RNA. These stages are initiation, elongation and termination, and they are well-understood biochemically. However, despite the plethora of structural information made available on RNA polymerase in the last decade, little is available for RNA polymerase in complex with transcription elongation factors. To understand the mechanisms of transcriptional regulation, we describe the first structure, to our knowledge, for a bacterial RNA polymerase in complex with an essential transcription elongation factor. The resulting structure formed between the RNA polymerase and NusA from Bacillus subtilis provides important insights into the transition from an initiation complex to an elongation complex, and how NusA is able to modulate transcription elongation and termination.
authors.affiliation: School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Seeseei
authors.initials: S
authors.lastname: Molimau
authors.forename: Geoff P
authors.initials: GP
authors.lastname: Doherty
authors.forename: Elecia B
authors.initials: EB
authors.lastname: Johnston
authors.forename: Jon
authors.initials: J
authors.lastname: Marles-Wright
authors.forename: Rosalba
authors.initials: R
authors.lastname: Rothnagel
authors.forename: Ben
authors.initials: B
authors.lastname: Hankamer
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Lewis
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Lewis
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008958:Models, Molecular; D010445:Peptide Elongation Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D010445:Peptide Elongation Factors; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit; C105078:RNA polymerase beta subunit
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