Determination of mangiferin in rat plasma by liquid-liquid extraction with UPLC-MS/MS.
An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method employing electrospray ionization (ESI) has been developed for the determination of mangiferin in rat plasma using diphenhydramine as the internal standard (IS). Liquid-liquid extraction (LLE) was used for sample preparation and the analysis was achieved with gradient elution on C(18) reversed phase column. The method was validated over the concentration range 0.02-5.0 microg/mL for oral administration and 0.4-100 microg/mL for intravenous administration. The intra-day and inter-day precision of mangiferin expressed as RSD<15% and the accuracy (RE) did not exceed 15%. This validated method is a novel technique for sample preparation and quantitation, which was successfully applied to estimate the bioavailability of mangiferin.
Han
Dandan
D
College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China.
Chen
Chengjun
C
Zhang
Cong
C
Zhang
Yu
Y
Tang
Xing
X
D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004155:Diphenhydramine / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D044004:Xanthones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D044004:Xanthones; C013592:mangiferin; D004155:Diphenhydramine