title: Bacillus marmarensis sp. nov., an alkaliphilic, protease-producing bacterium isolated from mushroom compost.
abstract: A Gram-stain-positive, obligately alkaliphilic bacterium designated strain GMBE 72(T) was isolated from mushroom compost from Yalova, located in the Marmara region of Turkey. Cells were aerobic, straight rods and they formed subterminal to terminal ellipsoidal endospores. The isolate was catalase-positive, oxidase-negative and motile and contained a type A1gamma peptidoglycan based on meso-diaminopimelic acid. The strain grew at pH 8.0-12.5. The major cellular fatty acid was anteiso-C(15 : 0). The genomic DNA G+C content was 40.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequencing showed that strain GMBE 72(T) belonged to the genus Bacillus and exhibited 98.2 % sequence similarity to Bacillus pseudofirmus DSM 8715(T). DNA-DNA reassociation was 56 % between GMBE 72(T) and B. pseudofirmus DSM 8715(T). According to our polyphasic characterization, strain GMBE 72(T) represents a novel species of the genus Bacillus, for which the name Bacillus marmarensis sp. nov. is proposed. The type strain is GMBE 72(T) (=DSM 21297(T) =JCM 15719(T)).
authors.affiliation: Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, PO Box 21, 41470 Gebze - Kocaeli, Turkey.
authors.forename: Aziz Akın
authors.initials: AA
authors.lastname: Denizci
authors.affiliation: Marmara University, Faculty of Engineering, Bioengineering Department, Göztepe Campus, 34722 Kadıköy, İstanbul, Turkey.|Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, PO Box 21, 41470 Gebze - Kocaeli, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Kazan
authors.affiliation: Kocaeli University, Faculty of Arts and Sciences, Department of Chemistry, Section of Biochemistry, Umuttepe Central Campus, 41380 İzmit - Kocaeli, Turkey.
authors.forename: Altan
authors.initials: A
authors.lastname: Erarslan
mesh_terms: D000363:Agaricales; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D001483:Base Sequence; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012987:Soil