The glutamate switch is present in all seven clades of AAA+ protein.
Recent work has identified a "glutamate switch" in six of the seven clades of AAA+ ATPases. The glutamate switch acts to transduce information regarding substrate binding to the ATPase active site. We provide biochemical evidence that a highly conserved threonine residue acts as a glutamate switch in the replicative helicase, MCM, and, thus, reveal that the glutamate switch is a feature common to all seven AAA+ clades.
Mogni
Maria Elena
ME
Sir William Dunn School of Pathology, Oxford University, South Parks Road, Oxford OX1 3RE, UK.
Costa
Alessandro
A
Ioannou
Charikleia
C
Bell
Stephen D
SD
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D020816:Amino Acid Motifs; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain / Q000235:genetics; D053488:DnaB Helicases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry* / Q000378:metabolism*; D026341:Minichromosome Maintenance 1 Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D040681:Structural Homology, Protein; D013379:Substrate Specificity / Q000235:genetics; D048229:Sulfolobus solfataricus / Q000201:enzymology; D013912:Threonine / Q000737:chemistry / Q000235:genetics
D019843:Archaeal Proteins; D026341:Minichromosome Maintenance 1 Protein; D013912:Threonine; D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D053488:DnaB Helicases
J Struct Biol. 2004 Apr-May;146(1-2):11-31
15037234
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16679413
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16116441
Structure. 2009 Feb 13;17(2):211-22
19217392
Microbiol Mol Biol Rev. 2006 Dec;70(4):876-87
17158702
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18208401
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16689629
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18849995
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19073923
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18523437