title: Platelet-derived growth factor is involved in the differentiation of second heart field-derived cardiac structures in chicken embryos.
abstract: For the establishment of a fully functional septated heart, addition of myocardium from second heart field-derived structures is important. Platelet-derived growth factors (PDGFs) are known for their role in cardiovascular development. In this study, we aim to elucidate this role of PDGF-A, PDGF-C, and their receptor PDGFR-alpha. We analyzed the expression patterns of PDGF-A, -C, and their receptor PDGFR-alpha during avian heart development. A spatiotemporal pattern of ligands was seen with colocalization of the PDGFR-alpha. This was found in second heart field-derived myocardium as well as the proepicardial organ (PEO) and epicardium. Mechanical inhibition of epicardial outgrowth as well as chemical disturbance of PDGFR-alpha support a functional role of the ligands and the receptor in cardiac development.
authors.affiliation: Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Noortje A M
authors.initials: NA
authors.lastname: Bax
authors.forename: Heleen
authors.initials: H
authors.lastname: Lie-Venema
authors.forename: Rebecca
authors.initials: R
authors.lastname: Vicente-Steijn
authors.forename: Steven B
authors.initials: SB
authors.lastname: Bleyl
authors.forename: Nynke M S
authors.initials: NM
authors.lastname: Van Den Akker
authors.forename: Saskia
authors.initials: S
authors.lastname: Maas
authors.forename: Robert E
authors.initials: RE
authors.lastname: Poelmann
authors.forename: Adriana C
authors.initials: AC
authors.lastname: Gittenberger-de Groot
mesh_terms: D000818:Animals; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000033:anatomy & histology / Q000196:embryology; D006801:Humans; D008222:Lymphokines / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; C411423:platelet-derived growth factor C; D020796:Receptor, Platelet-Derived Growth Factor alpha