Cu2+-selective naked-eye and fluorescent probe: its crystal structure and application in bioimaging.
A new fluorescent and colorimetric Cu2+ probe was synthesized, and realized optical imaging in RAW264.7 macrophages. The design strategy was based on a change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine-based dyes, and its crystal structure was presented to explain the binding mode. Upon treatment with Cu2+, the weakly fluorescent probe exhibited a strong fluorescence response, high selectivity and was quantitative for Cu2+ under physiological conditions. In addition, the off-on-type fluorescent change upon the addition of Cu2+ was also applied in bioimaging.
Yu
Fabiao
F
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Shandong Normal University, 88 East Wenhua Road, Jinan 250014, P. R. China.
Zhang
Wenshen
W
Li
Ping
P
Xing
Yanlong
Y
Tong
Lili
L
Ma
Jianping
J
Tang
Bo
B
D000818:Animals; D002460:Cell Line; D003124:Colorimetry / Q000379:methods*; D003300:Copper / Q000032:analysis*; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry
D005456:Fluorescent Dyes; D012235:Rhodamines; C026188:rhodamine 6G; D003300:Copper