Phyllaemblicin B inhibits Coxsackie virus B3 induced apoptosis and myocarditis.
Coxsackie virus B3 (CVB3) is believed to be a major contributor to viral myocarditis since virus-associated apoptosis plays a role in the pathogenesis of experimental myocarditis. In this study, we investigated the in vitro and in vivo antiviral activities of Phyllaemblicin B, the main ellagitannin compound isolated from Phyllanthus emblica, a Chinese herb medicine, against CVB3. Herein we report that Phyllaemblicin B inhibited CVB3-mediated cytopathic effects on HeLa cells with an IC(50) value of 7.75+/-0.15microg/mL. In an in vivo assay, treatment with 12mgkg(-1)d(-1) Phyllaemblicin B reduced cardiac CVB3 titers, decreased the activities of LDH and CK in murine serum, and alleviated pathological damages of cardiac muscle in myocarditic mice. Moreover, Phyllaemblicin B clearly inhibited CVB3-associated apoptosis effects both in vitro and in vivo. These results show that Phyllaemblicin B exerts significant antiviral activities against CVB3. Therefore, Phyllaemblicin B may represent a potential therapeutic agent for viral myocarditis.
Wang
Ya-Feng
YF
Institute of Pharmacology Science, Jinan University Guangdong, Guangzhou, China.
Wang
Xiao-Yan
XY
Ren
Zhe
Z
Qian
Chui-Wen
CW
Li
Yi-Cheng
YC
Kaio
Kitazato
K
Wang
Qing-Duan
QD
Zhang
Yan
Y
Zheng
Li-Yun
LY
Jiang
Jin-Hua
JH
Yang
Chong-Ren
CR
Liu
Qing
Q
Zhang
Ying-Jun
YJ
Wang
Yi-Fei
YF
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D003384:Coxsackievirus Infections / Q000188:drug therapy* / Q000821:virology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D006367:HeLa Cells; D006321:Heart / Q000821:virology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy* / Q000821:virology; D009206:Myocardium
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D006027:Glycosides; C418445:phyllaemblicin B