[Expression of B7-H1 molecule on human bone marrow mesenchymal stem cells and its effects on T lymphocyte proliferation].
The mechanisms of human bone marrow mesenchymal stem cells (hBMMSCs)-mediated immunomodulation are still not be completely clarified. In order to investigate the expression of B7-H1 on hBMMSCs and to explore whether B7-H1 mediated signaling pathway (B7-H1/PD-1) involves in the mechanisms of hBMMSCs-mediated immunomodulation, the hBMMSCs were isolated, cultured and identified, the B7-H1 expression on hBMMSCs was detected by flow cytometry, RT-PCR, and Western blot. The inhibitory effect of hBMMSCs on proliferation of T lymphocytes was observed in mixed lymphocyte culture, and then the functional anti-B7-H1 monoclonal antibody (mcAb) was used to block B7-H1, the proliferation of T lymphocytes was detected by using CCK-8. The results indicated that hBMMSCs highly expressed B7-H1 molecule, hBMMSCs effectively inhibited the proliferation of T lymphocytes with a dose-dependent manner, and the inhibitory proliferation of T lymphocytes by hBMMSCs could be partially restored when the anti-B7-H1 mAb was used to block the B7-H1, the inhibitory rate of T lymphocyte proliferation decreased from 64.1% to 38.75%. It is concluded that B7-H1 highly expresses on hBMMSCs, the B7-H1 mediated signaling pathway (B7-H1/PD-1) involves in the mechanisms for hBMMSCs-mediated immunomodulation.
Ni
Xun
X
Department of Hematology, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
Jia
Yong-Qian
YQ
Meng
Wen-Tong
WT
Zhong
Ling
L
Zeng
Yan
Y
D015703:Antigens, CD / Q000378:metabolism*; D060890:B7-H1 Antigen; D001854:Bone Marrow Cells / Q000378:metabolism*; D049109:Cell Proliferation; D006801:Humans; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology*
D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human