A convenient method for synthesis of glyconanoparticles for colorimetric measuring carbohydrate-protein interactions.
Carbohydrate functionalized nanoparticles, i.e., the glyconanoparticles, have wide application ranging from studies of carbohydrate-protein interactions, in vivo cell imaging, biolabeling, etc. Currently reported methods for preparation of glyconanoparticles require multi-step modifications of carbohydrates moieties to conjugate to nanoparticle surface. However, the required synthetic manipulations are difficult and time consuming. We report herewith a simple and versatile method for preparing glyconanoparticles. This method is based on the utilization of clean and convenient microwave irradiation energy for one-step, site-specific conjugation of unmodified carbohydrates onto hydrazide-functionalized Au nanoparticles. A colorimetric assay that utilizes the ensemble of gold glyconanoparticles and Concanavalin A (ConA) was also presented. This feasible assay system was developed to analyze multivalent interactions and to determine the dissociation constant (K(d)) for five kind of Au glyconanoparticles with lectin. Surface plasmon changes of the Au glyconanoparticles as a function of lectin-carbohydrate interactions were measured and the dissociation constants were determined based on non-linear curve fitting. The strength of the interaction of carbohydrates with ConA was found to be as follows: maltose>mannose>glucose>lactose>MAN5.
Chuang
Yen-Jun
YJ
Faculty of Engineering, University of Georgia, Athens, GA 30602, USA.
Zhou
Xichun
X
Pan
Zhengwei
Z
Turchi
Craig
C
D002241:Carbohydrates / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D003208:Concanavalin A / Q000737:chemistry; D006046:Gold; D006834:Hydrazines / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008872:Microwaves; D009844:Oligosaccharides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D002241:Carbohydrates; D006834:Hydrazines; D009844:Oligosaccharides; D011506:Proteins; D003208:Concanavalin A; D006046:Gold
Anal Biochem. 2004 Jul 1;330(1):145-55
15183773
Langmuir. 2008 Sep 16;24(18):10293-7
18715022
Carbohydr Res. 2002 Jan 7;337(1):21-9
11755908
J Am Chem Soc. 2003 May 21;125(20):6140-8
12785845
Biosens Bioelectron. 2006 Jan 15;21(7):1069-76
15935636
Anal Chem. 2002 Apr 1;74(7):1624-8
12033254
Analyst. 2001 Jan;126(1):71-8
11205517
Biochim Biophys Acta. 2006 Apr;1760(4):636-51
16529864
J Am Chem Soc. 2005 Nov 16;127(45):15791-800
16277522
Glycobiology. 1998 Mar;8(3):227-36
9451032
Science. 1997 Aug 22;277(5329):1078-81
9262471
Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):18-23
19106304
Chembiochem. 2004 Nov 5;5(11):1593-6
15515080
Anal Chem. 2008 Sep 15;80(18):7174-8
18707133
Org Biomol Chem. 2008 Jun 21;6(12):2095-102
18528571
Carbohydr Res. 1991 Jul 18;214(1):155-68
1954629
J Inorg Biochem. 1998 May;70(2):71-82
9666569
Langmuir. 2006 Jan 31;22(3):1156-63
16430279
Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10554-9
9380673
Nature. 2000 Feb 10;403(6770):669-72
10688205
Angew Chem Int Ed Engl. 2006 Apr 3;45(15):2348-68
16557636
Angew Chem Int Ed Engl. 1998 Nov 2;37(20):2754-2794
29711117
Carbohydr Res. 2007 Feb 26;342(3-4):448-59
17173881
Angew Chem Int Ed Engl. 2008;47(6):1054-7
18098262
J Am Chem Soc. 2007 Sep 12;129(36):11177-84
17705486
Langmuir. 2006 Jul 18;22(15):6707-11
16831017