[Tyrosinase inhibitor from Angelica dahurica roots].
OBJECTIVE
To isolate and identify the tyrosinase inhibitory constituent from Angelica dahurica.


METHODS
A tyrosinase inhibitory constituent was isolated by silica gel column chromatography combined with a bioassay-linked HPLC method.


RESULTS
An extract of heat-processed A. dahurica roots showed more potent tyrosinase inhibitory activity than that of the crude drug. Tyrosinase inhibitory furanocoumarin, 8-hydroxy-5-methoxypsoralen,was isolated. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid (IC50 = 0.0907 mmol x L(-1)) with IC50 value of 0.0086 mmol x L(-1). Therefore, it is contributive to the increased tyrosinase inhibitory activity of the heat-processed A. dahurica.


CONCLUSIONS
The active constituent was 8-hydroxy-5-methoxypsoralen from the heat-processed A. dahurica.
Piao
Xianglan
X
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China. xlpiao@edu.cn
D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D018517:Plant Roots / Q000737:chemistry*
D004791:Enzyme Inhibitors; D014442:Monophenol Monooxygenase