title: At4g24160, a soluble acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase.
abstract: Human CGI-58 (for comparative gene identification-58) and YLR099c, encoding Ict1p in Saccharomyces cerevisiae, have recently been identified as acyl-CoA-dependent lysophosphatidic acid acyltransferases. Sequence database searches for CGI-58 like proteins in Arabidopsis (Arabidopsis thaliana) revealed 24 proteins with At4g24160, a member of the alpha/beta-hydrolase family of proteins being the closest homolog. At4g24160 contains three motifs that are conserved across the plant species: a GXSXG lipase motif, a HX(4)D acyltransferase motif, and V(X)(3)HGF, a probable lipid binding motif. Dendrogram analysis of yeast ICT1, CGI-58, and At4g24160 placed these three polypeptides in the same group. Here, we describe and characterize At4g24160 as, to our knowledge, the first soluble lysophosphatidic acid acyltransferase in plants. A lipidomics approach revealed that At4g24160 has additional triacylglycerol lipase and phosphatidylcholine hydrolyzing enzymatic activities. These data establish At4g24160, a protein with a previously unknown function, as an enzyme that might play a pivotal role in maintaining the lipid homeostasis in plants by regulating both phospholipid and neutral lipid levels.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Ananda K
authors.initials: AK
authors.lastname: Ghosh
authors.forename: Neha
authors.initials: N
authors.lastname: Chauhan
authors.forename: Sona
authors.initials: S
authors.lastname: Rajakumari
authors.forename: Guenther
authors.initials: G
authors.lastname: Daum
authors.forename: Ram
authors.initials: R
authors.lastname: Rajasekharan
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D029681:Arabidopsis Proteins; D010743:Phospholipids; D011994:Recombinant Proteins; D014280:Triglycerides; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase
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