title: [Mapping of diagnostically important antigenic regions of major outer membrane protein (MOMP) from Chlamydia trachomatis using recombinant proteins with overlapping structures].
abstract: Amino acid sequence of the Chlamydia trachomatis major outer membrane protein (MOMP) was modeled using a series of the recombinant proteins containing six 100 aa fragments of MOMP overlapped in 30 aa. Testing of recombinant antigens in EIA showed that proteins containing MOMP fragments comprising 191-286 and 191-354 aa regions had the greatest activity in the reaction with anti- C. trachomatis positive serum samples. The data obtained allows the conclusion about the possibility of the use of presented recombinant proteins for development of diagnostic test for anti- C. trachomatis antibodies detection to be made.
authors.forename: N V
authors.initials: NV
authors.lastname: Savel'eva
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Zagriadskaia
authors.forename: S V
authors.initials: SV
authors.lastname: Klimashevskaia
authors.forename: V F
authors.initials: VF
authors.lastname: Puzyrev
authors.forename: A N
authors.initials: AN
authors.lastname: Burkov
authors.forename: A P
authors.initials: AP
authors.lastname: Obriadina
authors.forename: T I
authors.initials: TI
authors.lastname: Ulanova
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D018604:Epitope Mapping; D016133:Polymerase Chain Reaction; D018272:Porins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D011994:Recombinant Proteins; C071677:omp1 protein, Chlamydia trachomatis