title: Image-based adaptive optics for two-photon microscopy.
abstract: We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues.
authors.affiliation: Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, United Kingdom. delphine.debarre@polytechnique.edu
authors.forename: Delphine
authors.initials: D
authors.lastname: Débarre
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Botcherby
authors.forename: Tomoko
authors.initials: T
authors.lastname: Watanabe
authors.forename: Shankar
authors.initials: S
authors.lastname: Srinivas
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Booth
authors.forename: Tony
authors.initials: T
authors.lastname: Wilson
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D007089:Image Enhancement / Q000379:methods*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D055620:Optical Phenomena; D017785:Photons
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