Identification of Solanum habrochaites loci that quantitatively influence tomato fruit ripening-associated ethylene emissions.
The phytohormone ethylene is essential for ripening of climacteric fruits such as tomato. While many of the genes responsible for ethylene synthesis and perception have been identified, the regulatory network controlling autocatalytic climacteric ethylene synthesis is not well understood. In order to better understand the regulation of ripening-associated ethylene, we have exploited the genetic variation within Solanum Sect. Lycopersicon. In particular, we have used a near-isogenic population of S. habrochaites introgression lines to identify chromosome segments affecting ethylene emissions during ripening. S. habrochaites fruits produce much larger quantities of ethylene during ripening than do cultivated S. lycopersicum tomatoes. A total of 17 segments were identified; 3 had emissions more than twice the level of the tomato parent, 11 had less than a twofold increase and 3 had significantly reduced emissions at one or more ripening stages. While several of these segments co-segregate with known ethylene-related genes, many do not correspond to known genes. Thus, they may identify novel modes of regulation. These results illustrate the utility of wild relatives and their introgression lines to understand regulation of fruit ripening-related processes.
Dal Cin
Valeriano
V
University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL, 32611-0690, USA.
Kevany
Brian
B
Fei
Zhangjun
Z
Klee
Harry J
HJ
D005030:Ethylenes / Q000378:metabolism*; D005638:Fruit / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics*; D010937:Plant Growth Regulators / Q000235:genetics; D014835:Volatilization
D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene
Theor Appl Genet. 2005 Nov;111(7):1396-408
16177901
Plant Cell. 1991 Nov;3(11):1187-93
1821764
J Exp Bot. 2009;60(1):325-37
19088332
Plant Physiol. 1998 Dec;118(4):1295-305
9847103
Science. 2004 Sep 17;305(5691):1786-9
15375271
Genetics. 1995 Nov;141(3):1147-62
8582620
Science. 1991 Oct 18;254(5030):437-9
1925603
Mol Biotechnol. 2007 May;36(1):9-13
17827532
Plant J. 2007 Nov;52(4):627-39
17877702
Theor Appl Genet. 2007 Aug;115(3):429-42
17571252
Plant Cell. 2002 Jul;14(7):1457-67
12119367
Genetics. 2001 May;158(1):413-22
11333249
Plant Cell. 2002;14 Suppl:S131-51
12045274
Proc Int Conf Intell Syst Mol Biol. 1996;4:68-77
8877506
Plant Biotechnol J. 2003 May;1(3):195-207
17156032
Genetics. 1997 Oct;147(2):861-77
9335620
Theor Appl Genet. 1996 Jun;92(8):935-51
24166620
Plant Physiol. 1981 Dec;68(6):1428-32
16662121
Theor Appl Genet. 1996 Feb;92(2):213-24
24166170
Theor Appl Genet. 2004 Aug;109(3):523-33
15150691
Nat Biotechnol. 2006 Apr;24(4):447-54
16531992
J Exp Bot. 2006;57(4):887-96
16473892
Nature. 1972 May 26;237(5352):235-6
4557321
Plant J. 1996 Apr;9(4):525-35
8624515
Science. 1995 Dec 15;270(5243):1807-9
8525371
Genome. 2000 Oct;43(5):803-13
11081970
Theor Appl Genet. 2008 Mar;116(5):647-56
18185917
Theor Appl Genet. 2002 May;104(6-7):945-958
12582599
J Mol Biol. 1991 Dec 20;222(4):937-61
1762159