title: Localization and characteristics of DNA underreplication zone in the 75C region of intercalary heterochromatin in Drosophila melanogaster polytene chromosomes.
abstract: In Drosophila polytene chromosomes, regions of intercalary heterochromatin are scattered throughout the euchromatic arms. Here, we present data on the first fine analysis of the individual intercalary heterochromatin region, 75C1-2, located in the 3L chromosome. By using electron microscopy, we demonstrated that this region appears as three closely adjacent condensed bands. Mapping of the region on the physical map by means of the chromosomal rearrangements with known breakpoints showed that the length of the region is about 445 kb. Although it seems that the SUUR protein binds to the whole 75C1-2 region, the proximal part of the region is fully polytenized, so the DNA underreplication zone is asymmetric and located in the distal half of the region. Finally, we speculate that intercalary heterochromatin regions of Drosophila polytene chromosomes are organized into three different types with respect to the localization of the underreplication zone.
authors.affiliation: Department of Molecular and Cellular Biology, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Natalya G
authors.initials: NG
authors.lastname: Andreyenkova
authors.forename: Elena B
authors.initials: EB
authors.lastname: Kokoza
authors.forename: Valery F
authors.initials: VF
authors.lastname: Semeshin
authors.forename: Elena S
authors.initials: ES
authors.lastname: Belyaeva
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Demakov
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Pindyurin
authors.forename: Eugenia N
authors.initials: EN
authors.lastname: Andreyeva
authors.forename: Elena I
authors.initials: EI
authors.lastname: Volkova
authors.forename: Igor F
authors.initials: IF
authors.lastname: Zhimulev
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D053903:DNA Breaks, Double-Stranded; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism* / Q000648:ultrastructure; D006570:Heterochromatin / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D020161:Physical Chromosome Mapping; D021381:Protein Transport
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006570:Heterochromatin; C464850:SuUR protein, Drosophila
references.citation: Genetics. 2007 Sep;177(1):615-29
references.pmid: 17720900
references.citation: J Cell Sci. 2007 Jul 15;120(Pt 14):2344-51
references.pmid: 17606990
references.citation: Genes Dev. 2009 Mar 1;23(5):589-601
references.pmid: 19270159
references.citation: Methods Mol Biol. 2004;247:305-24
references.pmid: 14707355
references.citation: Cell. 1990 Oct 5;63(1):97-107
references.pmid: 2208283
references.citation: Genetics. 2003 Nov;165(3):1209-20
references.pmid: 14668376
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12819-24
references.pmid: 17640911
references.citation: Genetics. 2004 Jun;167(2):797-813
references.pmid: 15238529
references.citation: Chromosoma. 1981;84(1):33-47
references.pmid: 7297250
references.citation: Proc Natl Acad Sci U S A. 1939 Nov;25(11):571-7
references.pmid: 16577949
references.citation: Chromosoma. 1987;95(4):227-35
references.pmid: 3113852
references.citation: Chromosoma. 1985;91(3-4):210-33
references.pmid: 3920013
references.citation: Nucleic Acids Res. 2002 Sep 1;30(17):3698-705
references.pmid: 12202754
references.citation: Curr Biol. 2002 Jan 22;12(2):131-5
references.pmid: 11818064
references.citation: Nucleic Acids Res. 1994 Nov 25;22(23):5068-75
references.pmid: 7800501
references.citation: Adv Genet. 1998;37:1-566
references.pmid: 9352629
references.citation: Mol Cell Biol. 2000 Sep;20(17):6308-16
references.pmid: 10938107
references.citation: Genes Dev. 2004 Dec 15;18(24):3094-105
references.pmid: 15601823
references.citation: Chromosoma. 2006 Oct;115(5):355-66
references.pmid: 16583218
references.citation: Chromosome Res. 2008;16(6):851-62
references.pmid: 18704724
references.citation: Genetics. 1991 Nov;129(3):783-9
references.pmid: 1752422
references.citation: Chromosoma. 2001 Dec;110(7):487-500
references.pmid: 11862456
references.citation: J Cell Sci. 2005 Dec 1;118(Pt 23):5465-77
references.pmid: 16278293
references.citation: Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7532-7
references.pmid: 9636184
references.citation: J Cell Sci. 2003 Jan 1;116(Pt 1):169-76
references.pmid: 12456726
references.citation: Proc Natl Acad Sci U S A. 2005 Jun 7;102(23):8269-74
references.pmid: 15928082
references.citation: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D390-5
references.pmid: 15608223
references.citation: Chromosoma. 2003 Mar;111(6):377-98
references.pmid: 12644953
references.citation: Chromosoma. 2008 Oct;117(5):411-8
references.pmid: 18491121
references.citation: Genetics. 2002 Mar;160(3):1023-34
references.pmid: 11901119