Liquid chromatography-mass spectrometry in metabolite profiling.
Recent advances in the use of liquid chromatography-mass spectrometry for the study of metabolomics are reviewed. Sample preparations of biofluids and practical aspects of ultra-high pressure liquid chromatography are discussed. Applicability of different kinds of mass spectrometers for metabolite profiling is described. New tools-ion mobility spectroscopy and automated chip-based nanoelectrospray system with potentials to be applied in the metabolomics analysis are described.
Juo
Chiun-Gung
CG
Molecular Medicine Research Center, Chang Gung University, Kwei-san, Tao-yuan, Taiwan.
Chiu
Daniel Tsun-Yee
DT
Shiao
Ming-Shi
MS
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*