title: Fluxomics with ratiometric metabolite dyes.
abstract: Today's major excitement in biology centers on signaling: How can a cell or organism measure the myriad of environmental cues, integrate it, and acclimate to the new conditions? Hormonal signals and second messengers are in the focus of most of these studies, e.g., regulation of glucose transporter GLUT4 cycling by insulin, or regulation of plant growth by auxin or brassinosteroids.1-3 In comparison, we generally assume that we know almost everything about basic metabolism since it has been studied for many decades; for example we know since the early 80s that allosteric regulation by fructose-2,6-bisphophate plays an important role in regulating glycolysis in plants and animals.4 This may be the reason why studies of metabolism appear to be a bit out of fashion. But if we look to other organisms such as E. coli or yeast, we rapidly realize that metabolism is controlled by complex interconnected signaling networks, and that we understand little of these signaling networks in humans and plants.5,6 As it turns out, the cell registers many metabolites, and flux through the pathways is regulated using complex signaling networks that involve calcium as well as hormones.
authors.affiliation: Carnegie Institution; Stanford, California USA.
authors.forename: Bhavna
authors.initials: B
authors.lastname: Chaudhuri
authors.forename: Totte
authors.initials: T
authors.lastname: Niittylä
authors.forename: Friederike
authors.initials: F
authors.lastname: Hörmann
authors.forename: Wolf B
authors.initials: WB
authors.lastname: Frommer
references.citation: Microbiol Mol Biol Rev. 2006 Mar;70(1):253-82
references.pmid: 16524925
references.citation: Sci STKE. 2003 Feb 11;2003(169):PL4
references.pmid: 12582202
references.citation: Plant Physiol. 1987 Jun;84(2):201-4
references.pmid: 16665415
references.citation: J Biol Chem. 1985 Mar 25;260(6):3440-50
references.pmid: 3838314
references.citation: Plant Cell. 2001 Aug;13(8):1907-18
references.pmid: 11487701
references.citation: Nature. 1997 Aug 28;388(6645):882-7
references.pmid: 9278050
references.citation: J Biol Chem. 2006 Oct 13;281(41):30875-83
references.pmid: 16912038
references.citation: Yeast. 2004 Jul 15;21(9):769-79
references.pmid: 15282800
references.citation: Nat Methods. 2005 Aug;2(8):615-9
references.pmid: 16170893
references.citation: FEBS Lett. 2006 Oct 30;580(25):5885-93
references.pmid: 17034793
references.citation: Plant Cell. 2006 Sep;18(9):2314-25
references.pmid: 16935985
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9846-51
references.pmid: 12097642
references.citation: Biophys J. 2006 Mar 1;90(5):1790-6
references.pmid: 16339891
references.citation: J Biol Chem. 1997 May 16;272(20):13270-4
references.pmid: 9148946
references.citation: Science. 2004 Jan 2;303(5654):87-9
references.pmid: 14704427
references.citation: Plant Methods. 2006 Apr 06;2:7
references.pmid: 16600023
references.citation: J Fluoresc. 2004 Sep;14(5):603-9
references.pmid: 15617267
references.citation: Cell Res. 2006 May;16(5):427-34
references.pmid: 16699538
references.citation: Protein Sci. 2005 Sep;14(9):2304-14
references.pmid: 16131659
references.citation: J Biol Chem. 2005 Jun 3;280(22):21437-43
references.pmid: 15805107
references.citation: Curr Biol. 2006 Jun 6;16(11):R424-33
references.pmid: 16753558
references.citation: Mol Cell Biol. 2005 Dec;25(24):11102-12
references.pmid: 16314530
references.citation: J Biol Chem. 2006 Apr 28;281(17):11815-8
references.pmid: 16513634
references.citation: Trends Biochem Sci. 2004 Oct;29(10):556-64
references.pmid: 15450611
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9614-6
references.pmid: 12119424