At4g24160, a soluble acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase.
Human CGI-58 (for comparative gene identification-58) and YLR099c, encoding Ict1p in Saccharomyces cerevisiae, have recently been identified as acyl-CoA-dependent lysophosphatidic acid acyltransferases. Sequence database searches for CGI-58 like proteins in Arabidopsis (Arabidopsis thaliana) revealed 24 proteins with At4g24160, a member of the alpha/beta-hydrolase family of proteins being the closest homolog. At4g24160 contains three motifs that are conserved across the plant species: a GXSXG lipase motif, a HX(4)D acyltransferase motif, and V(X)(3)HGF, a probable lipid binding motif. Dendrogram analysis of yeast ICT1, CGI-58, and At4g24160 placed these three polypeptides in the same group. Here, we describe and characterize At4g24160 as, to our knowledge, the first soluble lysophosphatidic acid acyltransferase in plants. A lipidomics approach revealed that At4g24160 has additional triacylglycerol lipase and phosphatidylcholine hydrolyzing enzymatic activities. These data establish At4g24160, a protein with a previously unknown function, as an enzyme that might play a pivotal role in maintaining the lipid homeostasis in plants by regulating both phospholipid and neutral lipid levels.
Ghosh
Ananda K
AK
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Chauhan
Neha
N
Rajakumari
Sona
S
Daum
Guenther
G
Rajasekharan
Ram
R
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
D000214:Acyl Coenzyme A; D029681:Arabidopsis Proteins; D010743:Phospholipids; D011994:Recombinant Proteins; D014280:Triglycerides; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase
Microbiol Mol Biol Rev. 2000 Mar;64(1):153-79
10704478
Mol Biol Cell. 2006 Apr;17(4):1946-58
16436516
Can J Biochem Physiol. 1959 Aug;37(8):911-7
13671378
Gene Expr Patterns. 2004 Jul;4(4):413-21
15183308
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9508-13
15197253
FEBS Lett. 2005 Nov 7;579(27):6067-73
16226259
Plant Cell. 2005 Apr;17(4):1073-89
15772283
Curr Genet. 1989 Dec;16(5-6):339-46
2692852
Curr Opin Plant Biol. 2004 Jun;7(3):329-36
15134755
PLoS One. 2007 Aug 08;2(8):e718
17684564
Nucleic Acids Res. 2000 Jan 1;28(1):263-6
10592242
J Biol Chem. 2006 Apr 7;281(14):9210-8
16467296
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
J Biol Chem. 2008 Sep 5;283(36):24525-33
18606822
J Biol Chem. 2008 Apr 11;283(15):9768-75
18252723
Trends Plant Sci. 2005 Aug;10(8):368-75
16023886
Plant Physiol. 2001 Dec;127(4):1728-38
11743116
Curr Opin Struct Biol. 1999 Dec;9(6):732-7
10607665
Plant Physiol. 2004 Jan;134(1):129-36
14730067
Methods Enzymol. 1996;266:418-27
8743697
Science. 2001 Nov 30;294(5548):1942-5
11729323
Science. 1991 Apr 5;252(5002):80-7
17739077
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
J Bacteriol. 1998 Mar;180(6):1425-30
9515909
Biochim Biophys Acta. 1997 Sep 4;1348(1-2):10-6
9370311
Appl Microbiol Biotechnol. 2006 Jun;71(1):75-9
16493551
Plant Cell. 2006 Feb;18(2):321-39
16415209
J Biol Chem. 2001 Apr 6;276(14):10847-52
11283027
J Biol Chem. 2008 Apr 4;283(14):8846-54
18234677
Structure. 2006 Jul;14(7):1157-65
16843897
Plant Physiol. 2006 Aug;141(4):1533-43
16798944
EMBO J. 2004 Feb 11;23(3):572-81
14749726
J Biol Chem. 2001 Mar 30;276(13):10290-8
11139581
Mol Biol Evol. 2007 Aug;24(8):1596-9
17488738
Trends Plant Sci. 2004 Aug;9(8):378-84
15358268
J Biol Chem. 2004 Nov 19;279(47):48968-75
15364929
Plant Physiol. 2002 Mar;128(3):988-96
11891254
Appl Environ Microbiol. 2000 Nov;66(11):4883-9
11055939
Biochim Biophys Acta. 1968 Mar 4;152(2):307-15
4296335
Proc Natl Acad Sci U S A. 1988 Jun;85(12):4143-7
16593939
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
Science. 2006 Apr 14;312(5771):264-6
16614222
Plant Physiol. 2004 Mar;134(3):1206-16
14976237
J Lipid Res. 2009 Jan;50(1):3-21
18952573
Plant Cell. 2006 Mar;18(3):665-75
16473965