Influence of dietary calcium content on intestinal permeability in rat.
OBJECTIVE
Agents that increase the permeability of intestinal epithelium promote the absorption of nutrients by the gut. High calcium concentration in the gut has been shown to enhance passive transport of glucose in the rat intestine. An increase in the permeability of the intestinal epithelium may account for this observation. The present study was aimed at monitoring the permeability of intestine of rats fed high or low calcium diets.


METHODS
Everted intestinal sacs were used to study transports of substances across the gut. While radioactive and non radioactive calcium isotopes were employed to study the active transport and passive transport of calcium, transport of labelled mannitol was taken as a measure of passive permeability.


RESULTS
High calcium diet increased the passive transport of mannitol and calcium while decreasing the active transport of calcium by the everted gut sacs.


CONCLUSIONS
Passive mechanisms are enhanced by high calcium diet, while low calcium diet favours active transport. Calcium in the diet may be affecting intestinal transport.
Rao
J Prakasa
JP
Department of Pharmacology, Texas Tech Univeristy Health Sciences Center, Lubbock, TX, USA. jprao2001@gmail.com
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002136:Calcium, Dietary / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000502:physiology*; D004386:Duodenum / Q000033:anatomy & histology / Q000378:metabolism; D007082:Ileum / Q000033:anatomy & histology / Q000378:metabolism; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D002136:Calcium, Dietary; D008353:Mannitol; D002118:Calcium