Genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition.
In this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (PGP) traits at higher salt (NaCl) concentrations (2, 4, 6, and 8%). The results revealed that 24 rhizobacterial isolates were tolerant at 8% NaCl. Although all the 24 salt tolerable isolates produced indole-3-acetic acid (IAA), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. However, only three isolates showed the production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase. Diversity was analyzed through 16S rDNA-RFLP, and of these isolates with three tetra cutter restriction enzymes (HaeIII, AluI, and MspI), the representative cluster groups were identified by 16S rDNA sequencing. Bacillus and Bacillus-derived genera were dominant which showed PGP attributes at 8% NaCl concentration. Out of 24 isolates, nitrogen fixing ability (nif H gene) was detected in the two isolates, SU18 (Arthrobacter sp.) and SU48.
Upadhyay
Sudhir K
SK
Department of Environmental Sciences, B.B. Ambedkar University, Vidya Vihar, Rai Bareilly Road, Lucknow, Uttar Pradesh, 226 025, India. sudhirk7upadhyay@yahoo.co.in
Singh
Devendra P
DP
Saikia
Ratul
R
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism*; D012988:Soil Microbiology; D014908:Triticum / Q000254:growth & development / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
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