Changes in biomass activity and characteristics of activated sludge exposed to low ozone dose.
In this paper, the response mechanism of activated sludge exposed to low-dose ozone at less than 20mgO(3)g(-1) total suspended solids (TSS) was studied by analyzing the changes in sludge activity and the evolution of C, N, P and metals from sludge following ozonation. The intracellular ATP concentration was not affected at less than 5mgO(3)g(-1) TSS and thereafter decreased rapidly to around 60% when the ozone dose increased to 20mgO(3)g(-1) TSS. Similarly, the efficiency of sludge solubilization initially changed a little and then increased rapidly to around 30% at an ozone dose of 20mgO(3)g(-1) TSS. However, the activities of superoxide dismutase and protease decreased immediately upon exposure to ozone. These findings indicate that ozone firstly destroys the floc, leading to the disruption of the compact aggregates, which does not affect cells viability but induces a decrease in enzyme activities. Ozone then attacks the bacterial cells of the sludge, causing a decrease in cells viability. During ozonation, the content of carbon, nitrogen and phosphorus in the sludge matrix decreased, while the content of these elements in the micro-solids and supernatant gradually increased. Most of the released metals from the sludge matrix were found in the micro-solids.
Chu
Libing
L
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, China. chulibing@tsinghua.edu.cn
Wang
Jianlong
J
Wang
Bo
B
Xing
Xin-Hui
XH
Yan
Sangtian
S
Sun
Xulin
X
Jurcik
Benjamin
B
D000255:Adenosine Triphosphate / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D019216:Metals, Heavy / Q000378:metabolism; D010126:Ozone / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D013482:Superoxide Dismutase / Q000378:metabolism
D019216:Metals, Heavy; D012722:Sewage; D010126:Ozone; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D010447:Peptide Hydrolases