Investigation of memory responses following Plasmodium  chabaudi AS infection in mice distinct in susceptibility  to clinical malaria.
Plasmodium chabaudi chabaudi AS blood stage infection results in various degrees of clinical symptoms to malaria depending on the mouse strain. This study aimed to investigate the development of memory responses in susceptible A/J and resistant C57BL/6 mice which differ in the degree of susceptibility to clinical malaria following P. chabaudi AS infection. A rapid increase of activated cells (CD25(+), CD44(high), and CD62L(low)) and production of both interferon-gamma and interleukin-4 was found in spleens of both malaria-infected mouse strains. After the parasitemia had been cleared, these activated cells were converted to their resting phenotypes. Although exhibiting susceptible phenotype and having lower magnitude of cellular changes during primary infection, susceptible A/J mice that had been exposed to malaria parasites and drug-cured were able to generate protective immunity capable of control parasite growth following reinfection to the same level as C57BL/6 mice. This may be due to the capability of susceptible mice to produce parasite-specific antibodies, in particular of the IgG2a and IgG3 isotypes. The results from this study may provide more insights useful for the development of vaccines against malaria.
Wipasa
Jiraprapa
J
Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. jwipasa@chiangmai.ac.th
Hemsokana
Panida
P
Ruankham
Tunlaya
T
Hongsibsong
Surat
S
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D005260:Female; D018960:Hyaluronan Receptors / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D015847:Interleukin-4 / Q000378:metabolism; D019041:L-Selectin / Q000032:analysis; D016131:Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology; D008288:Malaria / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D016788:Plasmodium chabaudi / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000913:Antibodies, Protozoan; C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; C508018:Il2ra protein, mouse; D007074:Immunoglobulin G; D053645:Interleukin-2 Receptor alpha Subunit; D019041:L-Selectin; D015847:Interleukin-4; D007371:Interferon-gamma
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