title: Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.
abstract: A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands. M.Balk@nioo.knaw.nl
authors.forename: Melike
authors.initials: M
authors.lastname: Balk
authors.forename: Hans G H J
authors.initials: HG
authors.lastname: Heilig
authors.forename: Miriam H A
authors.initials: MH
authors.lastname: van Eekert
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: Stams
authors.forename: Irene C
authors.initials: IC
authors.lastname: Rijpstra
authors.forename: Jaap S
authors.initials: JS
authors.lastname: Sinninghe-Damsté
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.forename: Servé W M
authors.initials: SW
authors.lastname: Kengen
mesh_terms: D001482:Base Composition; D002248:Carbon Monoxide / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005285:Fermentation; D019015:Geologic Sediments / Q000382:microbiology*; D045482:Hot Springs / Q000382:microbiology*; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D046968:Thermoanaerobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014421:Turkey
chemical_list: D004269:DNA, Bacterial; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002248:Carbon Monoxide
references.citation: Mikrobiologiia. 2003 Mar-Apr;72(2):161-7
references.pmid: 12751237
references.citation: Int J Syst Evol Microbiol. 2002 Jul;52(Pt 4):1361-1368
references.pmid: 12148651
references.citation: Eur J Biochem. 1970 Jan;12(1):133-42
references.pmid: 4984993
references.citation: Arch Microbiol. 2003 May;179(5):315-20
references.pmid: 12637975
references.citation: Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1471-8
references.pmid: 10555328
references.citation: Antonie Van Leeuwenhoek. 1964;30:225-38
references.pmid: 14218435
references.citation: Biopolymers. 1980 Jul;19(7):1315-27
references.pmid: 7397315
references.citation: Syst Appl Microbiol. 1983;4(2):184-92
references.pmid: 23194591
references.citation: Extremophiles. 2007 Jan;11(1):145-57
references.pmid: 17021657
references.citation: Int J Syst Evol Microbiol. 2006 Apr;56(Pt 4):797-800
references.pmid: 16585697
references.citation: Appl Environ Microbiol. 2002 Apr;68(4):1914-8
references.pmid: 11916712
references.citation: Int J Syst Evol Microbiol. 2001 Jul;51(Pt 4):1539-1548
references.pmid: 11491356
references.citation: Nucleic Acids Res. 2004 Feb 25;32(4):1363-71
references.pmid: 14985472
references.citation: Int J Syst Bacteriol. 1995 Oct;45(4):783-9
references.pmid: 7547300
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: J Bacteriol. 2001 Sep;183(17):5134-44
references.pmid: 11489867
references.citation: Int J Syst Evol Microbiol. 2000 Nov;50 Pt 6:2141-2149
references.pmid: 11155990
references.citation: Anal Biochem. 1977 Aug;81(2):461-6
references.pmid: 907108
references.citation: Arch Microbiol. 1997 Aug;168(2):114-9
references.pmid: 9238102
references.citation: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2191-2193
references.pmid: 17911280
references.citation: Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):467-474
references.pmid: 15023962
references.citation: Appl Environ Microbiol. 1993 Apr;59(4):1114-9
references.pmid: 16348912
references.citation: Int J Syst Bacteriol. 1996 Jan;46(1):123-7
references.pmid: 8573487
references.citation: Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):2069-2073
references.pmid: 16166711
references.citation: Antonie Van Leeuwenhoek. 1995 Nov;68(4):309-15
references.pmid: 8821786
references.citation: Appl Environ Microbiol. 1988 Jun;54(6):1472-80
references.pmid: 16347658
references.citation: Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2353-2359
references.pmid: 15545483
references.citation: Extremophiles. 1997 Nov;1(4):171-82
references.pmid: 9680298
references.citation: Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):141-149
references.pmid: 11211251
references.citation: J Lipid Res. 1994 Jun;35(6):1057-65
references.pmid: 8077844
references.citation: Int J Syst Evol Microbiol. 2007 Jul;57(Pt 7):1429-1434
references.pmid: 17625170
references.citation: Int J Syst Evol Microbiol. 2002 Nov;52(Pt 6):1961-7
references.pmid: 12508854
references.citation: Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2565-73
references.pmid: 18984694
references.citation: Appl Environ Microbiol. 2004 Dec;70(12):7236-40
references.pmid: 15574922