PCR-RFLP analysis of chitinase genes enables efficient genotyping of Metarhizium anisopliae var. anisopliae.
A new genotyping tool has been developed and evaluated for Metarhizium anisopliae var. anisopliae. The tool is based on Restriction Fragment Length Polymorphism (RFLP) analysis of three chitinase genes that are functionally linked to insect-pathogenicity of this fungus. It allowed for discrimination of 14 genotypes among 22 M. anisopliae var. anisopliae strains of a world wide collection. Analyses revealed that the approach may also be applicable to other Metarhizium varieties. The new tool will be useful for genetic characterization of M. anisopliae var. anisopliae strains, and it is applicable for laboratories with limited access to molecular diagnostic equipment.
Enkerli
Jürg
J
Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, 8046 Zürich, Switzerland. juerg.enkerli@art.admin.ch
Ghormade
Vandana
V
Oulevey
Catherine
C
Widmer
Franco
F
D000818:Animals; D002688:Chitinases / Q000235:genetics*; D002965:Classification; D004271:DNA, Fungal / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D007300:Insect Control / Q000379:methods; D052981:Metarhizium / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D010572:Pest Control, Biological / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012988:Soil Microbiology; D013045:Species Specificity
D004271:DNA, Fungal; D002688:Chitinases