title: Gene expression profiles of lens regeneration and development in Xenopus laevis.
abstract: Seven hundred and thirty-four unique genes were recovered from a cDNA library enriched for genes up-regulated during the process of lens regeneration in the frog Xenopus laevis. The sequences represent transcription factors, proteins involved in RNA synthesis/processing, components of prominent cell signaling pathways, genes involved in protein processing, transport, and degradation (e.g., the ubiquitin/proteasome pathway), matrix metalloproteases (MMPs), as well as many other proteins. The findings implicate specific signal transduction pathways in the process of lens regeneration, including the FGF, TGF-beta, MAPK, Retinoic acid, Wnt, and hedgehog signaling pathways, which are known to play important roles in eye/lens development and regeneration in various systems. In situ hybridization revealed that the majority of genes recovered are expressed during embryogenesis, including in eye tissues. Several novel genes specifically expressed in lenses were identified. The suite of genes was compared to those up-regulated in other regenerating tissues/organisms, and a small degree of overlap was detected.
authors.affiliation: University of Illinois, Department of Cell and Developmental Biology, Urbana, Illinois 61801, USA.
authors.forename: Erica L
authors.initials: EL
authors.lastname: Malloch
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Perry
authors.forename: Lisa
authors.initials: L
authors.lastname: Fukui
authors.forename: Verity R
authors.initials: VR
authors.lastname: Johnson
authors.forename: Jason
authors.initials: J
authors.lastname: Wever
authors.forename: Caroline W
authors.initials: CW
authors.lastname: Beck
authors.forename: Michael W
authors.initials: MW
authors.lastname: King
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Henry
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007908:Lens, Crystalline / Q000196:embryology* / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism*
chemical_list: D029867:Xenopus Proteins; D020782:Matrix Metalloproteinases
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