Detection of myocardial injury in patients with unstable angina using a novel nanoparticle cardiac troponin I assay: observations from the PROTECT-TIMI 30 Trial.
BACKGROUND
At least 30% of patients with non-ST-elevation acute coronary syndrome present without evidence of myonecrosis using current generation troponin assays. A new generation of research assays for troponin that offer a >10-fold increase in analytical sensitivity has emerged.


METHODS
To perform a pilot study to evaluate the clinical sensitivity of a new ultra-sensitive nanoparticle assay for cardiac troponin I (nano-cTnI), we identified 50 patients with unstable angina (serial negative cTnI) and 50 patients with non-ST-elevation myocardial infarction with an initially negative current generation cTnI result. We measured cTnI using an assay (Nanosphere, Northbrook, IL) that can detect pg/mL concentrations of cTnI (detection-limit 0.0002 microg/L).


RESULTS
Measured at 0, 2, and 8 hours with the nano-cTnI assay 44%, 62%, and 82% of patients with unstable angina defined by the current-generation assay had an elevated nano-cTnI result (> or =0.003 microg/L, 99 th percentile decision-limit, coefficient of variation <10%). In patients with definite myocardial injury (current-generation cTnI > or =0.1 microg/L) but an initially negative cTnI, 72% and 98% had a nano-cTnI > or =0.003 microg/L at 0 and 2 hours. No patient had a positive current-generation cTnI without an elevated nano-cTnI level.


CONCLUSIONS
In this pilot study using a nanoparticle assay for cTnI, myocardial injury was detectable in a substantial proportion of patients presently classified as having unstable angina, suggesting that ischemia with rest pain without injury is rare. The emergence of a new generation of troponin assays has the potential to lead to new clinical applications based on enhanced analytical performance at very low concentrations of troponin.
Wilson
Sean R
SR
TIMI Study Group, Brigham and Women's Hospital and Department of Medicine, Boston, MA 02115, USA.
Sabatine
Marc S
MS
Braunwald
Eugene
E
Sloan
Sarah
S
Murphy
Sabina A
SA
Morrow
David A
DA
D054058:Acute Coronary Syndrome / Q000097:blood / Q000175:diagnosis / Q000628:therapy; D000368:Aged; D000789:Angina, Unstable / Q000097:blood* / Q000175:diagnosis* / Q000628:therapy; D015906:Angioplasty, Balloon, Coronary; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis* / Q000628:therapy; D009206:Myocardium / Q000378:metabolism; D053758:Nanoparticles; D010865:Pilot Projects; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000097:blood*
D019210:Troponin I