title: The functional domain grouping of microtubule associated proteins.
abstract: Microtubules (MTs), which play crucial roles in normal cell function, are regulated by MT associated proteins (MAPs). Using a combinatorial approach that includes biochemistry, proteomics and bioinformatics, we have recently identified 270 putative MAPs from Drosophila embryos and characterized some of those required for correct progression through mitosis. Here we identify functional groups of these MAPs using a reciprocal hits sequence alignment technique and assign InterPro functional domains to 28 previously uncharacterized proteins. This approach gives insight into the potential functions of MAPs and how their roles may affect MTs.
authors.affiliation: Department of Zoology; University of Oxford; Oxford United Kingdom.
authors.forename: Katherine H
authors.initials: KH
authors.lastname: Fisher
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Deane
authors.forename: James G
authors.initials: JG
authors.lastname: Wakefield
references.citation: Genome Res. 2004 Jun;14(6):1107-18
references.pmid: 15173116
references.citation: J Cell Biol. 1989 Dec;109(6 Pt 1):2977-91
references.pmid: 2574177
references.citation: Curr Biol. 1994 Feb 1;4(2):89-99
references.pmid: 7953530
references.citation: Mol Biol Cell. 2005 Feb;16(2):891-901
references.pmid: 15591130
references.citation: J Cell Biol. 2000 Jul 24;150(2):F63-8
references.pmid: 10908588
references.citation: Mol Biol Cell. 2008 Jul;19(7):3097-110
references.pmid: 18463163
references.citation: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D247-51
references.pmid: 16381856
references.citation: Cell Mol Life Sci. 2001 Dec;58(14):2085-97
references.pmid: 11814058
references.citation: J Cell Biol. 2002 Jul 22;158(2):305-19
references.pmid: 12119357
references.citation: Genes Cells. 2008 Apr;13(4):295-312
references.pmid: 18363962
references.citation: Prog Nucleic Acid Res Mol Biol. 2004;78:227-60
references.pmid: 15210332
references.citation: Genome Res. 2002 Jun;12(6):962-8
references.pmid: 12045149
references.citation: Science. 1991 May 24;252(5009):1162-4
references.pmid: 2031185
references.citation: Ann N Y Acad Sci. 1975 Jun 30;253:107-32
references.pmid: 1056738
references.citation: PLoS Biol. 2008 Apr 22;6(4):e98
references.pmid: 18433294
references.citation: Genetics. 1996 Mar;142(3):865-78
references.pmid: 8849893
references.citation: Int Rev Cytol. 2001;210:163-226
references.pmid: 11580206
references.citation: Handb Exp Pharmacol. 2008;(186):461-82
references.pmid: 18491064
references.citation: Mol Cell Proteomics. 2004 Oct;3(10):970-83
references.pmid: 15249590
references.citation: J Cell Biol. 2005 Oct 24;171(2):197-200
references.pmid: 16247021
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D201-5
references.pmid: 15608177
references.citation: J Cell Biol. 2000 Nov 13;151(4):905-18
references.pmid: 11076973
references.citation: EMBO J. 2000 Jan 17;19(2):241-52
references.pmid: 10637228
references.citation: Chromosoma. 1998 Dec;107(6-7):376-85
references.pmid: 9914369
references.citation: Science. 2004 Jul 2;305(5680):61-6
references.pmid: 15166316
references.citation: Genetics. 2002 Nov;162(3):1211-20
references.pmid: 12454067
references.citation: Genome Biol. 2003;4(3):R22
references.pmid: 12620107