Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces.
In this study, a total of 94 lactic acid bacterial (LAB) isolates of porcine small intestinal and fecal origin were screened for their probiotic properties. The aim was to evaluate whether their isolation site and putative species identity play a role in these characteristics and whether either of these can be used as a predictive factor for the probiotic potential of bacterial isolates. The isolates were preliminarily identified by partial 16S rRNA gene sequencing and characterized in vitro for their pH and bile tolerance, adhesion capacity towards porcine enterocytes isolated from five intestinal sites and for antimicrobial activity towards five indicator pathogens. The interdependence of these characteristics was statistically evaluated. The isolates tolerated low pH and bile well. Adherence to the enterocytes of different origins did not correlate with the strain isolation site. In general, higher adherence was observed to colon cells in comparison to the small intestinal enterocytes. Culture filtrates of the isolates caused a decrease of up to three orders of magnitude in the intestinal pathogen cell numbers. The inhibition was mostly due to lactic and other organic acids. The predominating phylotypes identified were Lactobacillus reuteri and Lactobacillus salivarius, of which the former generally had the best adhesion capacity, whereas the latter appeared to be the best inhibitor. Based on the results, several strains of the pig Lactobacillus isolates tested may function as promising candidates for use in probiotic products. However, it was not possible to use the isolation site or the species identity of the isolates as reliable preliminary screening factors.
Lähteinen
Tanja
T
Department of Basic Veterinary Sciences (DBVS), Division of Veterinary Microbiology and Epidemiology, Faculty of Veterinary Medicine, P.O. Box 66, University of Helsinki (UH), Helsinki 00014, Finland. tanja.lahteinen@helsinki.fi
Malinen
Erja
E
Koort
Joanna M K
JM
Mertaniemi-Hannus
Ulla
U
Hankimo
Tanja
T
Karikoski
Ninja
N
Pakkanen
Soile
S
Laine
Hanna
H
Sillanpää
Hanna
H
Söderholm
Henna
H
Palva
Airi
A
D000818:Animals; D001422:Bacterial Adhesion; D020895:Enterocytes / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology*; D019936:Probiotics; D013552:Swine