The influence of membrane physical properties on microvesicle release in human erythrocytes.
Exposure of human erythrocytes to elevated intracellular calcium causes fragments of the cell membrane to be shed as microvesicles. This study tested the hypothesis that microvesicle release depends on microscopic membrane physical properties such as lipid order, fluidity, and composition. Membrane properties were manipulated by varying the experimental temperature, membrane cholesterol content, and the activity of the trans-membrane phospholipid transporter, scramblase. Microvesicle release was enhanced by increasing the experimental temperature. Reduction in membrane cholesterol content by treatment with methyl-beta-cyclodextrin also facilitated vesicle shedding. Inhibition of scramblase with R5421 impaired vesicle release. These data were interpreted in the context of membrane characteristics assessed previously by fluorescence spectroscopy with environment-sensitive probes such as laurdan, diphenylhexatriene, and merocyanine 540. The observations supported the following conclusions: 1) calcium-induced microvesicle shedding in erythrocytes relates more to membrane properties detected by diphenylhexatriene than by the other probes; 2) loss of trans-membrane phospholipid asymmetry is required for microvesicle release.PACS Codes: 87.16.dj, 87.16.dt.
Gonzalez
Laurie J
LJ
Department of Physiology and Developmental Biology, Brigham Young University Provo, Utah 84602, USA. john_bell@byu.edu.
Gibbons
Elizabeth
E
Bailey
Rachel W
RW
Fairbourn
Jeremy
J
Nguyen
Thaothanh
T
Smith
Samantha K
SK
Best
Katrina B
KB
Nelson
Jennifer
J
Judd
Allan M
AM
Bell
John D
JD
Biochim Biophys Acta. 1980 Feb 15;596(1):108-17
7353003
J Biol Chem. 1996 Jul 19;271(29):17205-10
8663431
Blood Cells. 1987;12(3):565-88
3651613
Biochim Biophys Acta. 1994 Mar 23;1190(2):217-24
8142419
J Immunol. 1992 Apr 1;148(7):2207-16
1545126
J Biol Chem. 1987 Mar 5;262(7):3291-9
3818642
Eur J Biochem. 1983 Jan 17;130(1):19-25
6297895
Arterioscler Thromb Vasc Biol. 2007 Feb;27(2):414-21
17110600
J Biol Chem. 2001 Jun 22;276(25):22732-41
11294854
Biochem J. 1998 Aug 1;333 ( Pt 3):591-9
9677317
J Lipid Res. 2008 Jun;49(6):1202-15
18299615
Biochim Biophys Acta. 1993 Feb 23;1146(1):136-44
8443220
J Biol Chem. 1999 Apr 23;274(17):11494-504
10206954
Apoptosis. 2007 Sep;12(9):1703-20
17610065
Biophys J. 2006 Dec 1;91(11):4091-101
16980369
Biophys J. 2007 Oct 1;93(7):2350-62
17545239
Biophys J. 1994 Mar;66(3 Pt 1):763-8
8011908
Biophys J. 2005 Apr;88(4):2692-705
15681653
Biochim Biophys Acta. 1992 Jun 30;1107(2):245-54
1504069
J Clin Invest. 1988 Aug;82(2):394-400
3403710
Adv Cancer Res. 2007;98:149-90
17433910
Biochemistry. 1998 Nov 3;37(44):15383-91
9799499
Biophys J. 2004 Apr;86(4):2251-60
15041664
Blood. 1992 Jan 15;79(2):380-8
1730083
J Immunol. 1994 May 15;152(10):4832-42
8176206
Biochim Biophys Acta. 2002 Sep 20;1565(1):123-8
12225860
Biochemistry. 2002 Nov 26;41(47):13982-8
12437355
Biochem J. 1981 Sep 15;198(3):433-40
6798976
FEBS Lett. 1999 Mar 5;446(1):113-6
10100625
Biochim Biophys Acta. 1990 Jul 24;1026(2):153-60
2116169
Immunol Lett. 1995 Dec;48(3):159-66
8867846
Blood. 2002 Apr 1;99(7):2569-77
11895795
J Leukoc Biol. 2001 Sep;70(3):405-12
11527990
Int J Hematol. 2000 Oct;72(3):298-309
11185985
Can J Physiol Pharmacol. 1996 Jun;74(6):706-11
8909783
Blood. 1998 Mar 15;91(6):2133-8
9490700
Biophys J. 1992 May;61(5):1427-34
1600086
Biophys J. 2008 Apr 15;94(8):3084-93
18192373
J Biol Chem. 1997 Jul 18;272(29):18240-4
9218461
Mol Immunol. 2007 Mar;44(9):2292-302
17173971
Biochim Biophys Acta. 2007 Jun;1768(6):1311-24
17493580
J Biol Chem. 1977 Nov 10;252(21):7500-10
914824
Biochemistry. 2001 Aug 7;40(31):9438-46
11478914
Scanning Microsc. 1996;10(3):795-803; discussion 803-4
9813640
J Mol Biol. 1977 Dec 25;117(4):1109-13
606835
Biophys J. 1991 Jul;60(1):179-89
1883937
Acta Biochim Pol. 2000;47(3):565-78
11310960
J Immunol Methods. 2002 Mar 1;261(1-2):129-39
11861071
J Membr Biol. 1981 Mar 15;59(1):57-63
6787204
J Struct Biol. 2005 May;150(2):200-10
15866743
Blood. 1997 Feb 15;89(4):1121-32
9028933
Br J Haematol. 1997 Dec;99(4):959-67
9432050