A novel GABAergic afferent input to the pontine reticular formation: the mesopontine GABAergic column.
Pharmacological manipulations of gamma-aminobutyric acid (GABA) neurotransmission in the nucleus pontis oralis (PnO) of the rat brainstem produce alterations in sleep/wake behavior. Local applications of GABA(A) receptor antagonists and agonists increase REM sleep and wake, respectively. These findings support a role for GABAergic mechanisms of the PnO in the control of arousal state. We have been investigating sources of GABA innervation of the PnO that may interact with local GABA(A) receptors in the control of state. Utilizing a retrograde tracer, cholera toxin-B subunit (CTb), injected into the PnO and dual-label immunohistochemistry with an antibody against glutamic acid decarboxalase-67 (GAD67), we report on a previously unidentified GABAergic neuronal population projecting to the contralateral PnO appearing as a column of cells, with long-axis in the sagittal plane, extending through the midbrain and pons. We refer to these neurons as the mesopontine GABAergic column (MPGC). The contiguous, columnar, anatomical distribution suggests operation as a functional neural system, which may influence expression of REM sleep, wake and other behaviors subserved by the PnO.
Liang
Chang-Lin
CL
Department of Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical Center, Dallas, TX 75216, USA.
Marks
Gerald A
GA
D000344:Afferent Pathways / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D001931:Brain Mapping / Q000379:methods; D002772:Cholera Toxin / Q000378:metabolism; D005133:Eye Movements / Q000502:physiology; D007839:Functional Laterality / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism*; D056347:Neuroanatomical Tract-Tracing Techniques / Q000379:methods; D056325:Neuronal Tract-Tracers / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011149:Pons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012154:Reticular Formation / Q000166:cytology / Q000378:metabolism*; D012890:Sleep / Q000502:physiology; D014851:Wakefulness / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D056325:Neuronal Tract-Tracers; D005680:gamma-Aminobutyric Acid; D002772:Cholera Toxin; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
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