title: In-vitro anthelminthic activity of crude aqueous extracts of Aloe ferox, Leonotis leonurus and Elephantorrhiza elephantina against Haemonchus contortus.
abstract: Aloe ferox (Mill), Leonotis leonurus (L) R. BR; and Elephantorrhiza elephantina (Burch.) Skeels are plants frequently used by resource-limited farmers in the Eastern Cape Province to control gastrointestinal parasites in goats. A study was conducted to validate their anthelminthic activities in-vitro on the egg and larvae of the nematode parasite Haemonchus contortus. The crude aqueous extracts of leaves of A. ferox and L. leonurus; and roots of E. elephantina were used. Eggs and larvae of the parasite were incubated at 25 degrees C in aqueous extracts at concentrations of 0.625-20 mg/ml for 48 h and 7 days for the egg hatch and larval development assays respectively. Albendazole and water were the positive and negative controls respectively. Inhibition of egg hatching and larval development increased significantly (P < 0.05) with increasing concentrations of the extracts. E. elephantina and L. leonurus extracts had 100% egg hatch inhibition at concentration as low as 2.5 mg/ml and 1.25 mg/ml respectively, whereas A. ferox extracts had 100% inhibition at concentrations of 20 mg/ml. At the lowest concentration tested (0.625 mg/ml), E. elephantina inhibited egg hatching >96% and this was comparable to albendazole at the same concentration. E. elephantina and L. leonurus also totally inhibited larval development at concentrations of 1.25 mg/ml. The study provided evidence that A. ferox, E. elephantina and L. leonurus extracts possess anthelminthic activity, thus justifying their use in the treatment of GI helminthosis. There is however need to assess the safety of these plants in vivo and also to undertake in vivo efficacy studies.
authors.affiliation: Department of Livestock and Pasture Sciences, University of Fort Hare, Private Bag X 1314, Alice, 5700, South Africa.
authors.forename: Viola
authors.initials: V
authors.lastname: Maphosa
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Masika
authors.forename: Edmund S
authors.initials: ES
authors.lastname: Bizimenyera
authors.forename: J N
authors.initials: JN
authors.lastname: Eloff
mesh_terms: D000504:Aloe / Q000737:chemistry; D000818:Animals; D007887:Fabaceae / Q000737:chemistry; D005767:Gastrointestinal Diseases / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D015511:Goat Diseases / Q000188:drug therapy / Q000469:parasitology*; D006041:Goats; D006188:Haemonchiasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000254:growth & development; D019686:Lamiaceae / Q000737:chemistry; D007814:Larva / Q000254:growth & development; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D013019:South Africa; D018709:Statistics, Nonparametric
chemical_list: D010936:Plant Extracts
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