Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood-derived mesenchymal stem cells.
Live imaging is a powerful technique that can be used to characterize the fate and location of stem cells in animal models. Here we investigated the characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, MNPs@SiO2(RITC). We also conducted various in vivo-uptake tests with nanoparticle-labeled human MSCs. MNPs@SiO2(RITC) showed photostability against ultraviolet light exposure and were nontoxic to human MSCs, based on the MTT, apoptosis, and cell cycle arrest assays. In addition, MNPs@SiO2(RITC) did not affect the surface phenotype or morphology of human MSCs. We also demonstrated that MNPs@SiO2(RITC) have stable retention properties in MSCs in vitro. Furthermore, using optical and magnetic resonance imaging, we successfully detected a visible signal from labeled human MSCs that were transplanted into NOD.CB17-Prkdc(SCID) (NOD-SCID) mice. These results demonstrate that MNPs@SiO2(RITC) are biocompatible and useful tools for human MSC labeling and bioimaging.


BACKGROUND
The characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, RITC were investigated in this study. RITC showed photostability against ultraviolet light exposure and was nontoxic to human MSCs. Using both optical and magnetic resonance imaging, successful detection of signal from labeled human MSCs transplanted into mice is demonstrated.
Park
Ki-Soo
KS
Department of Molecular Medicine, Sungkyunkwan University, Seoul, South Korea.
Tae
Jinsung
J
Choi
Bongkum
B
Kim
Young-Seok
YS
Moon
Cheol
C
Kim
Sa-Hyun
SH
Lee
Han-Sin
HS
Kim
Jinhyun
J
Kim
Junsung
J
Park
Jaeberm
J
Lee
Jung-Hee
JH
Lee
Jong Eun
JE
Joh
Jae-Won
JW
Kim
Sungjoo
S
D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry*; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D053758:Nanoparticles / Q000009:adverse effects*; D012235:Rhodamines / Q000009:adverse effects; D012822:Silicon Dioxide / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
D003287:Contrast Media; D004337:Drug Carriers; D012235:Rhodamines; D012822:Silicon Dioxide; C027755:rhodamine isothiocyanate