title: Evaluation of potential genotoxicity of virgin olive oil (VOO) using the drosophila wing-spot test.
abstract: Edible and nonedible grades of virgin olive oil (VOO), differing in quality characteristics, were evaluated for potential genotoxicity in the Drosophila somatic mutation and recombination test (SMART) before and after heating at high temperatures. Drosophila larvae were fed on medium containing 6 and 12% v/v of each of the examined oils. Edible VOOs did not exhibit any mutagenic or recombination activity even after thermal treatment. Lower grade VOO gave negative results at the concentration of 6% and inconclusive ones at 12%. However, after its thermal treatment, a statistically significant increase of large single spots was observed, giving a positive result for this spot category at both concentrations. Evaluation of the possible contribution of olive phenolic compounds to the nongenotoxic effects observed was carried out using a polar olive leaf extract and pure oleuropein. No significant increase in the frequency of any category of mutant spots was recorded for leaf extract (0.8-12 mg of total polar phenols/dose) or pure oleuropein (0.8-8 mg/dose). These results are expected to contribute to the ongoing interest in the inherent properties of VOO as part of the everyday diet.
authors.affiliation: Department of Genetics, Faculty of Science, School of Biology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors.forename: Ilias
authors.initials: I
authors.lastname: Kounatidis
authors.forename: Vassiliki T
authors.initials: VT
authors.lastname: Papoti
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Nenadis
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Franzios
authors.forename: Mariangella
authors.initials: M
authors.lastname: Oikonomou
authors.forename: Fedra
authors.initials: F
authors.lastname: Partheniou
authors.forename: Maria
authors.initials: M
authors.lastname: Tsimidou
authors.forename: Penelope
authors.initials: P
authors.lastname: Mavragani-Tsipidou
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D005260:Female; D007814:Larva / Q000187:drug effects / Q000235:genetics; D008297:Male; D016296:Mutagenesis / Q000187:drug effects; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D031658:Olea / Q000737:chemistry; D000069463:Olive Oil; D010936:Plant Extracts / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity*; D011995:Recombination, Genetic / Q000187:drug effects; D014921:Wings, Animal
chemical_list: D009153:Mutagens; D000069463:Olive Oil; D010936:Plant Extracts; D010938:Plant Oils