title: [Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC].
abstract: OBJECTIVE
  To develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum.
  
  
  METHODS
  Agilent TC-C18 column (4.6 mm x 250 mm, 5 microm) was used at 30 degrees C with the mobile phase of methanol-water (75:25). The flow rate was set at 0.8 mL x min(-1). The detection wavelength was 321 nm.
  
  
  RESULTS
  The linear response ranged from 3.20-28.80 microg for +/- praeruptorin A (r = 0.9999, n = 5), 1.60-14.40 g for praeruptorin B (r = 0.9995, n = 5) and 1.64-14.76 g for qianhucoumarin E (r = 0.9994, n = 5), respectively. Recoveries were 98.92% with RSD 1.6% for praeruptorin A, 99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E.
  
  
  CONCLUSIONS
  The method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.
authors.affiliation: Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Wu
authors.forename: Jiajia
authors.initials: J
authors.lastname: Chen
authors.forename: Shoujin
authors.initials: S
authors.lastname: Liu
authors.forename: Nianjun
authors.initials: N
authors.lastname: Yu
authors.forename: Chengwu
authors.initials: C
authors.lastname: Fang
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis* / Q000302:isolation & purification; D016014:Linear Models; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D003374:Coumarins; C030123:coumarin