title: [Effect of topotecan on MAGE gene expression of HPB-AM cells].
abstract: OBJECTIVE
  To investigate whether topotecan, a novel anti-tumor agent, down-regulates gene expression of melanoma antigen-encoding (MAGE) in HPB-AM cells.
  
  
  METHODS
  MAGE mRNA expression of HPB-AM cells was detected by RT-PCR 4, 8, 12 and 16 hrs after different concentrations (0.05, 0.10, 0.15 and 0.20 micromol/L) of topotecan treatment.
  
  
  RESULTS
  MAGE mRNA expression of HPB-AM cells decreased with increasing concentrations of topotecan 12 hrs after treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10, 0.15 and 0.20 micromol/L of topotecan was significantly lower than that in the blank control group (P<0.05). MAGE mRNA expression of HPB-AM cells was significantly reduced in a time-dependent manner after 0.10 micromol/L of topotecan treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10 micromol/L of topotecan was significantly lower than that in the blank control group 12 and 16 hrs after treatment (P<0.05).
  
  
  CONCLUSIONS
  Topotecan is capable of inhibiting the expression of MAGE mRNA of HPB-AM cells in a time- and dose-dependent manner.
authors.affiliation: Department of Pediatrics, General Hospital of People's Liberation Army, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lei
authors.forename: Suo-Qin
authors.initials: SQ
authors.lastname: Tang
authors.forename: Tian-You
authors.initials: TY
authors.lastname: Wang
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D019772:Topotecan / Q000494:pharmacology*
chemical_list: D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; C072013:MAGEA3 protein, human; C095204:MAGEA4 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D019772:Topotecan