[Effects of alcohol and high-fat diet on the expression of phosphatidylinositol 3-kinase in rat adipose tissue].
OBJECTIVE
To investigate the effects of alcohol and high-fat diet on the expression of PI-3K-P85alpha in rat adipose tissue, and in order to explore the effects on insulin sensitivity and the molecular mechanisms induced by combination of alcohol and high-fat diet.


METHODS
The rats were treated by alcohol and high-fat. Then the contents of fasting plasma glucose and insulin, and HOMA-IR index of each group were calculated. Expression of PI-3K-P85alpha were detectedby RT-PCR and Western blotting.


RESULTS
In comparison with normal diet group, HOMA-IR index of high-fat groups increased, in comparison with high-fat control group, the contents of fasting plasma glucose increased in alcohol combined with high-fat groups, and HOMA-IR increased both in the low-dose and middle-dose alcohol combined with high-fat model groups. Expressions of PI-3K-P85alpha remarkably decreased in all the alcohol and high-fat model groups (P < 0.05).


CONCLUSIONS
High-fat diet could induce insulin resistance. Compared with high-fat diet alone, alcohol combined with high-fat could aggravate insulin resistance. The changes of PI-3K-P85alpha expression in adipose tissue may be one of the molecular mechanism of insulin resistance induced by alcohol combined high-fat diet.
Yu
Dong
D
Department of Nutrion and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Peng
Xiaolin
X
Zhao
Lina
L
Tian
Liyue
L
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects*; D007333:Insulin Resistance; D008297:Male; D058539:Phosphatidylinositol 3-Kinase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D012333:RNA, Messenger; D000431:Ethanol; D058539:Phosphatidylinositol 3-Kinase