Differential expression of proteins in Listeria monocytogenes under thermotolerance-inducing, heat shock, and prolonged heat shock conditions.
Listeria monocytogenes is a foodborne pathogen capable of employing stress adaptive responses to evade a variety of stressors including temperature stress. We employed two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight analysis to study the differential expression of L. monocytogenes (ATCC 43256) soluble proteins at heat shock (60 degrees C) conditions, prolonged heat shock (60 degrees C for 9 minutes) conditions, and thermotolerance-inducing (48 degrees C for 30 minutes followed by 60 degrees C for 9 minutes) conditions. We compared the proteome of L. monocytogenes under these conditions to the proteome at 37 degrees C. Eighteen proteins were differentially expressed at 60 degrees C (6 up-regulated and 12 down-regulated), 21 proteins were differentially expressed (12 up-regulated and 9 down-regulated) when the cells were exposed to 60 degrees C for 9 minutes, and 20 proteins were differentially expressed (10 up-regulated and 10 down-regulated) when cells were initially exposed to 48 degrees C for 30 minutes before 60 degrees C for 9 minutes. There was one unidentifiable protein with observed molecular weight of 50 kDa which was differentially expressed across the three temperature treatments. Thermotolerance-inducing conditions caused the up-regulation of a protein by as much as 12-fold. DnaN, a previously identified stress protein, was up-regulated almost threefold at 60 degrees C. TcsA, a lipoprotein (CD4 T cell-stimulating antigen), and Gap (glyceraldehyde-3-phosphate-dehydrogenase) were selectively expressed under prolonged heat shock conditions suggesting their potential as candidate marker proteins targets for identifying temperature-stressed L. monocytogenes cells.
Agoston
Réka
R
Corvinus University of Budapest, Budapest, Hungary.
Soni
Kamlesh
K
Jesudhasan
Palmy R
PR
Russell
William K
WK
Mohácsi-Farkas
Csilla
C
Pillai
Suresh D
SD
D001426:Bacterial Proteins / Q000378:metabolism*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D006358:Hot Temperature / Q000009:adverse effects*; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological; D015854:Up-Regulation
D001426:Bacterial Proteins; D020543:Proteome