title: Postnatal expression of GAD67.
abstract: N-methyl-D-aspartate receptor blockade promotes apoptosis at postnatal day 7 (P7) and is linked to loss of glutamic acid decarboxylase 67 (GAD67) expression in older animals. To more fully appreciate this relationship we must first understand how GAD67 is regulated postnatally. Thus, the brains of P7, P14 and P21 rats were examined for expression of GAD67 protein and we found that levels of this GABAergic marker increased steadily with age, such that by P21 there was as much as a 6-fold increase compared to P7 animals and a 1.5- to 2-fold increase compared to P14 animals, depending on the region sampled. At P7, GAD67 was almost exclusively detected in puncta, with very few cell bodies displaying this marker. In contrast, at P14 and especially P21, both puncta and cell bodies were robustly labeled. Our data indicate that adult-like expression of GAD67 emerges quite late in the postnatal period.
authors.affiliation: Neurobiology and Anatomy, Wake Forest University School of Medicine, 1 Medical Center Blvd, Winston-Salem, NC 27157-1010, USA. cpturner@wfubmc.edu
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Turner
authors.forename: Emily
authors.initials: E
authors.lastname: Ware
authors.forename: Robert
authors.initials: R
authors.lastname: Stowe
authors.forename: Danielle
authors.initials: D
authors.lastname: DeBenedetto
authors.forename: Caroline
authors.initials: C
authors.lastname: Walburg
authors.forename: Andrew
authors.initials: A
authors.lastname: Lee
authors.forename: John
authors.initials: J
authors.lastname: Swanson
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lambert
authors.forename: Melissa
authors.initials: M
authors.lastname: Lyle
authors.forename: Priyanka
authors.initials: P
authors.lastname: Desai
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology*; D005968:Glutamate Decarboxylase / Q000096:biosynthesis*; D006179:Gyrus Cinguli / Q000201:enzymology; D017072:Neostriatum / Q000201:enzymology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013003:Somatosensory Cortex / Q000201:enzymology
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
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