Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G.C.G.C tetrad.
Short contiguous arrays of variant CTAGGG repeats in the human telomere are unstable in the male germline and somatic cells, suggesting formation of unusual structures by this repeat type. Here, we report on the structure of an intramolecular G-quadruplex formed by DNA sequences containing four human telomeric variant CTAGGG repeats in potassium solution. Our results reveal a new robust antiparallel G-quadruplex fold involving two G-tetrads sandwiched between a G.C base pair and a G.C.G.C tetrad, which could represent a new platform for drug design targeted to human telomeric DNA.
Lim
Kah Wai
KW
Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore.
Alberti
Patrizia
P
Guédin
Aurore
A
Lacroix
Laurent
L
Riou
Jean-François
JF
Royle
Nicola J
NJ
Mergny
Jean-Louis
JL
Phan
Anh Tuân
AT
D002151:Calorimetry; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D054856:G-Quadruplexes; D014644:Genetic Variation; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011188:Potassium / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet; D016615:Telomere / Q000737:chemistry*; D013816:Thermodynamics
D011188:Potassium
Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):18938-43
16365301
Structure. 1993 Dec 15;1(4):263-82
8081740
Bioorg Med Chem Lett. 2005 Apr 15;15(8):2047-50
15808465
Nucleic Acids Res. 2008 Oct;36(17):5482-515
18718931
Nucleic Acids Res. 2005 Aug 16;33(14):4649-59
16106044
FEBS J. 2007 Jul;274(14):3545-3556
17561958
Methods Enzymol. 2001;338:341-71
11460557
J Am Chem Soc. 2006 Aug 2;128(30):9963-70
16866556
Nucleic Acids Res. 2006;34(19):5715-9
17040899
J Mol Biol. 1998 Sep 25;282(3):637-52
9737927
Nucleic Acids Res. 2006 May 19;34(9):2723-35
16714449
Biochimie. 2008 May;90(5):686-96
18294461
Nucleic Acids Res. 2007;35(19):6517-25
17895279
Nucleic Acids Res. 2007;35(15):4927-40
17626043
Bioorg Med Chem. 2006 Aug 15;14(16):5584-91
16682210
Nucleic Acids Res. 2005 Oct 12;33(18):5851-60
16221978
J Biomol NMR. 2000 Feb;16(2):175-8
10723997
Biopolymers. 1970;9(9):1059-77
5449435
J Mol Biol. 1998 Sep 25;282(3):619-36
9737926
Nucleic Acids Res. 2005 Jul 21;33(13):4065-77
16040598
Nucleic Acids Res. 2005 Sep 12;33(16):e138
16157860
J Am Chem Soc. 2009 Apr 1;131(12):4301-9
19271707
J Am Chem Soc. 2007 Sep 12;129(36):11185-91
17705383
Nucleic Acids Res. 1989 Jun 26;17(12):4611-27
2664709
J Mol Biol. 2000 May 26;299(1):123-44
10860727
Nucleic Acids Res. 2006 May 08;34(8):2386-97
16682446
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14629-34
14645716
J Am Chem Soc. 2005 Jul 6;127(26):9439-47
15984871
EMBO J. 1995 Nov 1;14(21):5433-43
7489732
Nucleic Acids Res. 2009 Oct;37(18):6225-38
19656953
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6622-6
3413114
Nucleic Acids Res. 1997 Dec 1;25(23):4758-63
9365253
Nucleic Acids Res. 2005 Jun 02;33(10):3185-92
15933211
Nucleic Acids Res. 2008 Jul;36(12):4079-87
18535007
FEBS Lett. 1998 Sep 11;435(1):74-8
9755862
J Am Chem Soc. 2002 Feb 20;124(7):1160-1
11841271
Nature. 2002 Jun 20;417(6891):876-80
12050675
Nucleic Acids Res. 2005 Apr 07;33(6):2022-31
15817566
Nucleic Acids Res. 2003 Mar 15;31(6):1605-13
12626701
Eur J Cancer. 1997 Apr;33(5):735-49
9282112
Nucleic Acids Res. 2009 Feb;37(3):931-8
19103662
Oligonucleotides. 2003;13(6):515-37
15025917
J Mol Biol. 1995 Dec 8;254(4):638-56
7500339
J Mol Biol. 2001 Oct 5;312(5):1073-88
11580251
J Magn Reson. 2003 Jan;160(1):65-73
12565051
Nucleic Acids Res. 2007;35(9):3064-75
17452368
Nucleic Acids Res. 2007;35(7):2440-50
17395643
Biochimie. 2008 Jan;90(1):131-55
17822826