Inflammation and taste disorders: mechanisms in taste buds.
Taste disorders, including taste distortion and taste loss, negatively impact general health and quality of life. To understand the underlying molecular and cellular mechanisms, we set out to identify inflammation-related molecules in taste tissue and to assess their role in the development of taste dysfunctions. We found that 10 out of 12 mammalian Toll-like receptors (TLRs), type I and II interferon (IFN) receptors, and their downstream signaling components are present in taste tissue. Some TLRs appear to be selectively or more abundantly expressed in taste buds than in nongustatory lingual epithelium. Immunohistochemistry with antibodies against TLRs 1, 2, 3, 4, 6, and 7 confirmed the presence of these receptor proteins in taste bud cells, of which TLRs 2, 3, and 4 are expressed in the gustducin-expressing type II taste bud cells. Administration of TLR ligands, lipopolysaccharide, and double-stranded RNA polyinosinic:polycytidylic acid, which mimics bacterial or viral infection, activates the IFN signaling pathways, upregulates the expression of IFN-inducible genes, and downregulates the expression of c-fos in taste buds. Finally, systemic administration of IFNs augments apoptosis of taste bud cells in mice. Taken together, these data suggest that TLR and IFN pathways function collaboratively in recognizing pathogens and mediating inflammatory responses in taste tissue. This process, however, may interfere with normal taste transduction and taste bud cell turnover and contributes to the development of taste disorders.
Wang
Hong
H
Monell Chemical Senses Center, Philadelphia, Pennsylvania 19104, USA. hwang@monell.org
Zhou
Minliang
M
Brand
Joseph
J
Huang
Liquan
L
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007249:Inflammation / Q000150:complications / Q000503:physiopathology*; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013650:Taste Buds / Q000503:physiopathology*; D013651:Taste Disorders / Q000150:complications / Q000503:physiopathology*; D051193:Toll-Like Receptors / Q000235:genetics / Q000502:physiology
D017931:DNA Primers; D051193:Toll-Like Receptors
J Neurosci. 2003 Sep 3;23(22):7967-73
12954857
Endocrinology. 2008 Sep;149(9):4402-12
18499758
Am J Physiol Gastrointest Liver Physiol. 2007 Jun;292(6):G1770-83
17395901
J Contemp Dent Pract. 2003 Feb 15;4(1):10-31
12595930
N C Med J. 1997 Mar-Apr;58(2):100-4
9088135
Gastroenterology. 1976 Apr;70(4):568-71
1254140
Oncol Nurs Forum. 1999 May;26(4):697-706
10337648
Handb Exp Pharmacol. 2008;(183):21-50
18071653
Pharmacol Ther. 2003 Oct;100(1):1-29
14550502
Cancer. 1986 Apr 15;57(8 Suppl):1709-15
3948143
Sci STKE. 2007 Sep 04;2007(402):pe47
17785714
Cell. 1994 Jun 17;77(6):829-39
8004672
Semin Immunol. 2007 Feb;19(1):24-32
17275323
Mol Immunol. 2007 May;44(12):3100-11
17403538
Ann Otol Rhinol Laryngol. 2003 Nov;112(11):971-8
14653367
Nat Neurosci. 1999 Dec;2(12):1055-62
10570481
Nature. 1995 Oct 19;377(6550):591-4
7566171
Clin J Oncol Nurs. 2002 Mar-Apr;6(2):73-7
11889680
Cell. 1994 Jan 28;76(2):253-62
8293462
Med Clin North Am. 1999 Jan;83(1):57-74
9927960
J Neurosci. 2007 Oct 3;27(40):10703-13
17913904
Annu Rev Immunol. 2005;23:307-36
15771573
Ann Otol Rhinol Laryngol. 1975 Sep-Oct;84(5 Pt 1):672-82
1190677
Physiol Behav. 1995 Aug;58(2):287-93
7568432
Biochem Biophys Res Commun. 2005 Dec 23;338(3):1402-9
16263085
Cancer Immunol Immunother. 1989;28(3):213-7
2784356
Am J Otolaryngol. 1983 May-Jun;4(3):187-216
6349404
Eur J Clin Nutr. 2004 Apr;58(4):629-36
15042131
J Biol Chem. 2006 Oct 13;281(41):31002-11
16880211
Physiol Behav. 1995 Jan;57(1):89-96
7878130
Nature. 1995 Jul 6;376(6535):37-43
7596430
Brain Res Bull. 1997;44(2):155-65
9292205
Behav Brain Res. 2004 May 5;151(1-2):73-82
15084423
Postgrad Med. 1987 Jan;81(1):251-7, 260
3809040