title: Tethering of ferredoxin:NADP+ oxidoreductase to thylakoid membranes is mediated by novel chloroplast protein TROL.
abstract: Working in tandem, two photosystems in the chloroplast thylakoid membranes produce a linear electron flow from H(2)O to NADP(+). Final electron transfer from ferredoxin to NADP(+) is accomplished by a flavoenzyme ferredoxin:NADP(+) oxidoreductase (FNR). Here we describe TROL (thylakoid rhodanese-like protein), a nuclear-encoded component of thylakoid membranes that is required for tethering of FNR and sustaining efficient linear electron flow (LEF) in vascular plants. TROL consists of two distinct modules; a centrally positioned rhodanese-like domain and a C-terminal hydrophobic FNR binding region. Analysis of Arabidopsis mutant lines indicates that, in the absence of TROL, relative electron transport rates at high-light intensities are severely lowered accompanied with significant increase in non-photochemical quenching (NPQ). Thus, TROL might represent a missing thylakoid membrane docking site for a complex between FNR, ferredoxin and NADP(+). Such association might be necessary for maintaining photosynthetic redox poise and enhancement of the NPQ.
authors.affiliation: Department of Molecular Biology, Ruder Bosković Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
authors.forename: Snjezana
authors.initials: S
authors.lastname: Jurić
authors.forename: Kroata
authors.initials: K
authors.lastname: Hazler-Pilepić
authors.forename: Ana
authors.initials: A
authors.lastname: Tomasić
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Lepedus
authors.forename: Branka
authors.initials: B
authors.lastname: Jelicić
authors.forename: Sujith
authors.initials: S
authors.lastname: Puthiyaveetil
authors.forename: Tihana
authors.initials: T
authors.lastname: Bionda
authors.forename: Lea
authors.initials: L
authors.lastname: Vojta
authors.forename: John F
authors.initials: JF
authors.lastname: Allen
authors.forename: Enrico
authors.initials: E
authors.lastname: Schleiff
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Fulgosi
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D004579:Electron Transport / Q000502:physiology; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000502:physiology; D016415:Sequence Alignment; D015398:Signal Transduction; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; C547211:TROL protein, Arabidopsis; D005287:Ferredoxin-NADP Reductase