Subtractive proteomic approach to the endometrial carcinoma invasion front.
Tumor invasion defines the transition between tissue-restricted carcinomas, related to good outcome as optimal surgery becomes possible, and metastatic tumors associated with poor prognosis and a dramatic decrease in survival. In endometrial cancer, myometrial infiltration represents a determinant parameter highly valuable in prognosis. To date, the identification of proteins involved in endometrial carcinoma invasion has been essentially conducted by immunohistochemical methods, without a global perception on the invasive front. Laser microdissection presents nowadays limitations to the profound spatiotemporal regulation from both the tumor and the surrounding stroma occurring at the invasive front. In this work, we attempted an alternative proteomic approach to characterize specific components of the tumor invasive front or its reactive stroma, by comparing the invasive area of an endometrial carcinoma with the noninvasive superficial tumor area and normal tissue from the same patients. This strategy led us to identify proteins involved in cellular morphology, assembly and movement, differentially expressed at the invasive front, as well as pathways like cell-to-cell signaling and interaction and a modulated response to oxidative stress as events related to endometrial carcinoma invasion. In conclusion, we could identify new players of myometrial infiltration by applying a subtractive proteomic approach to the endometrial carcinoma invasion front.
Monge
Marta
M
Biomedical Research Unit, Research Institute Vall d'Hebron University Hospital, Pg. Vall d'Hebron 119-129, 08035 Barcelona, Spain.
Doll
Andreas
A
Colas
Eva
E
Gil-Moreno
Antonio
A
Castellvi
Josep
J
Garcia
Angel
A
Colome
Nuria
N
Perez-Benavente
Asummpcio
A
Pedrola
Nuria
N
Lopez-Lopez
Rafael
R
Dolcet
Xavier
X
Ramon y Cajal
Santiago
S
Xercavins
Jordi
J
Matias-Guiu
Xavier
X
Canals
Francesc
F
Reventos
Jaume
J
Abal
Miguel
M
D000368:Aged; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D016889:Endometrial Neoplasms / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism; D000072105:Superoxide Dismutase-1
D011506:Proteins; D020543:Proteome; C000606290:SOD1 protein, human; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019789:biliverdin reductase