title: In vivo analysis of the Notch receptor S1 cleavage.
abstract: A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lake
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Grimm
authors.forename: Alexey
authors.initials: A
authors.lastname: Veraksa
authors.forename: Andrew
authors.initials: A
authors.lastname: Banos
authors.forename: Spyros
authors.initials: S
authors.lastname: Artavanis-Tsakonas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D004330:Drosophila; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D021381:Protein Transport; D051880:Receptors, Notch / Q000737:chemistry / Q000378:metabolism*
chemical_list: D051880:Receptors, Notch
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