Differential dynamics of donor DC and non-DC peripheral blood mononuclear cell microchimerism in lung transplantation.
Donor cell microchimerism induces tolerance in animal models and may increase graft survival in man. Since dendritic cells (DC) are critical for induction of both tolerance and alloreactivity we developed a method to quantitate microchimerism in donor DC and non-DC in peripheral blood mononuclear cells (PBMC) after lung transplantation. Longitudinal analysis of donor cell microchimerism in eleven sex mismatched lung transplant recipients (LTR) up to 12 months post-transplant used Y chromosome based real-time PCR on sorted cells. Total DC or a proportion of DC subsets in PBMC did not change but there were heterogeneous and dynamic changes in microchimerism in DC and non-DC. Analysis of changes in DC using a mixed model analysis showed significantly less reduction in DC compared to non-DC over time (0.49, p=0.001). Preferential DC persistence compared to non-DC may indicate tolerance induction but future studies are required to determine if DC microchimerism after transplantation affects clinical outcomes.
Van der Meulen
Joel
J
Department of Medicine, Monash University, Melbourne, Australia.
Mifsud
Nicole A
NA
Abud
Diahnn
D
Paul
Eldho
E
Varney
Michael D
MD
Lewin
Sharon R
SR
Cameron
Paul U
PU
Kotsimbos
Tom C
TC
D046528:Chimerism; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005260:Female; D050173:Genes, Y-Linked / Q000235:genetics; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D016040:Lung Transplantation / Q000276:immunology*; D008297:Male; D014184:Transplantation, Homologous / Q000276:immunology