title: Software for muscle fibre type classification and analysis.
abstract: Fibre type determination requires a large series of differently stained muscle sections. The manual identification of individual fibres through the series is tedious and time consuming. This paper presents a software that enables (i) adjusting the position of individual fibres through a series of differently stained sections (image registration) and identification of individual fibres through the series as well as (ii) muscle fibre classification and (iii) quantitative analysis. The data output of the system is the following: numerical and areal proportions of fibre types, fibre type size and optical density (grey level) of the final reaction product in every fibre. The muscle fibre type can be determined stepwise, based on one set of stained sections while further, newly stained sections can be added to the already defined muscle fibre profile. Several advantages of the presented software application in skeletal muscle research are presented. The system is semiquantitative, flexible, and user friendly.
authors.affiliation: Department of Biomathematics, Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Karen
authors.forename: M
authors.initials: M
authors.lastname: Stevanec
authors.forename: V
authors.initials: V
authors.lastname: Smerdu
authors.forename: E
authors.initials: E
authors.lastname: Cvetko
authors.forename: L
authors.initials: L
authors.lastname: Kubínová
authors.forename: I
authors.initials: I
authors.lastname: Erzen
mesh_terms: D000818:Animals; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008406:Masseter Muscle / Q000166:cytology; D018485:Muscle Fibers, Skeletal / Q000145:classification* / Q000166:cytology*; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D009218:Myosins / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D012984:Software; D014584:User-Computer Interface
chemical_list: D020033:Protein Isoforms; D018995:Myosin Heavy Chains; D009218:Myosins
references.citation: J Anat. 1995 Oct;187 ( Pt 2):403-11
references.pmid: 7592003
references.citation: Histochem Cell Biol. 1996 Nov;106(5):473-9
references.pmid: 8950605
references.citation: Biochem Biophys Res Commun. 2000 May 27;272(1):303-8
references.pmid: 10872844
references.citation: Am J Physiol. 1997 Jun;272(6 Pt 1):C1881-9
references.pmid: 9227417
references.citation: J Muscle Res Cell Motil. 1996 Aug;17(4):401-9
references.pmid: 8884596
references.citation: J Muscle Res Cell Motil. 2001;22(8):647-55
references.pmid: 12222825
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2958-63
references.pmid: 10077619
references.citation: Arch Oral Biol. 2001 Nov;46(11):1039-50
references.pmid: 11543711
references.citation: J Neurosci Res. 2000 Mar 1;59(5):692-7
references.pmid: 10686598
references.citation: Cytometry. 1999 Oct 1;37(2):93-106
references.pmid: 10486521
references.citation: Pflugers Arch. 1996;431(6 Suppl 2):R211-2
references.pmid: 8739339
references.citation: Muscle Nerve. 1991 Apr;14(4):304-9
references.pmid: 1827511
references.citation: Histochemistry. 1991;95(6):579-83
references.pmid: 1856112
references.citation: Arch Neurol. 1970 Oct;23(4):369-79
references.pmid: 4248905
references.citation: Microsc Res Tech. 2009 Feb;72(2):110-9
references.pmid: 19003887
references.citation: J Neurol Sci. 1994 Feb;121(2):194-202
references.pmid: 8158215
references.citation: Histochem J. 1999 May;31(5):277-83
references.pmid: 10461862
references.citation: Eur J Histochem. 2008 Jul-Sep;52(3):179-90
references.pmid: 18840559
references.citation: Med Biol Eng Comput. 2001 Jan;39(1):51-5
references.pmid: 11214273
references.citation: Eur J Histochem. 1998;42(1):49-62
references.pmid: 9615191
references.citation: Meat Sci. 1992;32(3):267-78
references.pmid: 22059813
references.citation: J Muscle Res Cell Motil. 1989 Jun;10(3):197-205
references.pmid: 2547831
references.citation: Muscle Nerve. 2000 May;23(5):661-79
references.pmid: 10797389
references.citation: Cytometry. 1998 Aug 1;32(4):317-26
references.pmid: 9701401
references.citation: Am J Physiol. 1994 Dec;267(6 Pt 1):C1723-8
references.pmid: 7545970
references.citation: J Biophys Biochem Cytol. 1961 Jan;9:47-61
references.pmid: 13729759
references.citation: Histochemistry. 1984;80(5):429-33
references.pmid: 6237078