Human coagulated plasma as a natural and low cost matrix for in vitro angiogenesis.
Angiogenesis, the development of new blood vessels, is an important process in tissue development and wound healing, but becomes pathologic when associated with solid tumor growth, proliferative retinopathies, and rheumatoid arthritis. Accurate and reliable qualification of neovascular (angiogenic) response, both in vitro and in vivo, is an essential requirement for the study of new blood vessel growth. The complexity of currently used three-dimensional in vitro angiogenesis systems makes it difficult to approve material in its models. Capillary-like structure occurs on basement membrane components such as collagen and/or laminin, while in other models, CLS formation occurs on transitional matrices such as fibrin. To solve this problem, we were interested in developing an angiogenesis system which allows rapid and reliable quantification of three-dimensional neovessel formation in vitro.



Human bone marrow endothelial cells were seeded on gelatin-coated microcarriers and suspended in a solution of platelet-poor plasma which was induced to polymerize by addition of calcium chloride. In this way, microcarriers were entrapped in three-dimensional coagulated plasma.



Within a few hours, endothelial cells begin to leave these supporting microcarries and migrate into the coagulated-plasma matrix and formed CLS within 48-72 hours.



We developed a convenient angiogenesis in vitro system which allows reliable quantification of capillary formation in a three-dimensional environment.
Mansouri
Kamran
K
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Mostafaei
Ali
A
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Mirshahi
Manochehr
M
Faculty of Basic Sciences, Tarbiat Modarres University, Iran.
Mohammadi Motlagh
Hamidreza
H
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Maleki
Ali
A
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Keshavarz
Maryam
M
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
D043925:Angiogenesis Inducing Agents / Q000737:chemistry / Q000494:pharmacology; D001688:Biological Products / Q000737:chemistry / Q000191:economics / Q000494:pharmacology; D001777:Blood Coagulation / Q000502:physiology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D002196:Capillaries / Q000254:growth & development / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003362:Cost-Benefit Analysis; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D010949:Plasma / Q000166:cytology / Q000502:physiology*; D054457:Tissue Scaffolds / Q000191:economics
D043925:Angiogenesis Inducing Agents; D001688:Biological Products