[The growth promoting activity of two different kinds of reconstructed terminators in transgenic common carp].
The transgenic promoter directly affects the activity of transgenic gene. Choice of terminator has been proved to affect the activity of transgenic gene. Since the relevant theoretical research is rare, using the terminator of transgenic gene or the promoter gene to construct the transgenic vector has not come to the conclusion. In order to construct a better transgenic vector to nurture "all fish" growth hormone transgenic fish which has a rapid growth character, two different transgenic vectors were constructed separately by the terminator of beta-actin gene and the terminator of growth hormone gene. After micro-injection, we obtained the P0 generation of "all fish" transgenic fish farming groups. Comparing the activities of the two different transgenic vectors, the weight distribution of the breeding groups from growth hormone gene terminator transgenic vector felt into a normal distribution; however, the weight distribution of the breeding groups originated from beta-actin gene terminator exhibited a non-normal mode and in a rightist trend. The average weight of GH (Growth hormone) gene terminator transgenic groups was significantly higher than that of the beta-actin gene terminator transgenic groups. Interestingly, a fast-growth transgenic carp was found from a mixed farming group, which was positive for the growth hormone gene terminator. This result showed that "all fish" GH gene transgenic carp could grow fast, and the transgenic gene could inherit from generation to generation. Our results suggest that the growth hormone gene terminator transgenic vector has stronger growth-promoting activity than the beta-actin gene terminator transgenic vector.
Zhong
Shan
S
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. zhongshan@whu.edu.cn
Luo
Da-Ji
DJ
Wu
Gang
G
Xu
Jing
J
Wang
Ya-Ping
YP
Zhu
Zuo-Yan
ZY
D000199:Actins / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D002347:Carps / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism; D013728:Terminator Regions, Genetic
D000199:Actins; D013006:Growth Hormone