title: Antiobesity and antidiabetic effects of biotransformed blueberry juice in KKA(y) mice.
abstract: OBJECTIVE
  Biotransformation of blueberry juice by the Serratia vaccinii bacterium gave rise to adenosine monophosphate-activated protein kinase (AMPK) phosphorylation and glucose uptake in muscle cells and adipocytes, but inhibited adipogenesis. This study investigated the antiobesity and antidiabetic potential of biotransformed blueberry juice (BJ) in KKA(y) mice, rodent model of leptin resistance.
  
  
  METHODS
  BJ was incorporated in drinking water of KKA(y) mice. Parameters of body weight, food intake, plasma glucose, insulin, leptin, and adiponectin were measured. Before and after therapy, animals were subjected to an oral glucose tolerance test. At the end of treatment, liver, muscle, kidney, epididymal fat pad, abdominal fat pad, and dorsal fat pad were collected and weighed.
  
  
  RESULTS
  Incorporating BJ in drinking water protected young KKA(y) mice from hyperphagia and significantly reduced their weight gain. Moreover, BJ protected young KKA(y) mice against the development of glucose intolerance and diabetes mellitus. Chronic BJ administration in obese and diabetic KKA(y) mice reduced food intake and body weight. This effect could not fully explain the associated antidiabetic effect because BJ-treated mice still showed lower blood glucose level when compared with pair-fed controls. The adipokines pathway also seems to be involved because BJ significantly increased adiponectin levels in obese mice.
  
  
  CONCLUSIONS
  This study shows that BJ decreases hyperglycemia in diabetic mice, at least in part by reversing adiponectin levels. BJ also protects young pre-diabetic mice from developing obesity and diabetes. Thus, BJ may represent a novel complementary therapy and a source of novel therapeutic agents against diabetes mellitus.
authors.affiliation: Department of Pharmacology, Université de Montréal, Montréal, Quebec, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Vuong
authors.forename: A
authors.initials: A
authors.lastname: Benhaddou-Andaloussi
authors.forename: A
authors.initials: A
authors.lastname: Brault
authors.forename: D
authors.initials: D
authors.lastname: Harbilas
authors.forename: L C
authors.initials: LC
authors.lastname: Martineau
authors.forename: D
authors.initials: D
authors.lastname: Vallerand
authors.forename: C
authors.initials: C
authors.lastname: Ramassamy
authors.forename: C
authors.initials: C
authors.lastname: Matar
authors.forename: P S
authors.initials: PS
authors.lastname: Haddad
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000818:Animals; D001628:Beverages; D029798:Blueberry Plants; D001835:Body Weight; D003920:Diabetes Mellitus / Q000097:blood* / Q000517:prevention & control; D006943:Hyperglycemia / Q000097:blood / Q000517:prevention & control*; D006963:Hyperphagia / Q000517:prevention & control; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D020738:Leptin / Q000097:blood*; D008297:Male; D051379:Mice; D009765:Obesity / Q000097:blood / Q000517:prevention & control*
chemical_list: D052242:Adiponectin; D007004:Hypoglycemic Agents; D020738:Leptin