Intrinsic expression of host genes and intronic miRNAs in prostate carcinoma cells.
BACKGROUND
Recent data show aberrant and altered expression of regulatory noncoding micro (mi) RNAs in prostate cancer (PCa). A large number of miRNAs are encoded in organized intronic clusters within many protein coding genes. While expression profiling studies of miRNAs are common place, little is known about the host gene and their resident miRNAs coordinated expression in PCa cells. Furthermore, whether expression of a subset of miRNAs is distinct in androgen-responsive and androgen-independent cells is not clear. Here we have examined the expression of mature miRNAs of miR 17-92, miR 106b-25 and miR 23b-24 clusters along with their host genes C13orf25, MCM7 and AMPO respectively in PCa cell lines.


RESULTS
The expression profiling of miRNAs and host genes was performed in androgen-sensitive MDA PCa 2b and LNCaP as well as in androgen-refractory PC-3 and DU 145 cell culture models of PCa. No significant correlation between the miRNA expression and the intrinsic hormone-responsive property of PCa cells was observed. Androgen-sensitive MDA PCa 2b cells exhibited the highest level of expression of most miRNAs studied in this report. We found significant expression variations between host genes and their resident miRNAs. The expressions of C13orf25 and miR 17-92 cluster as well as MCM7 and miR 106b-25 cluster did not reveal statistically significant correlation, thus suggesting that host genes and resident miRNAs may be expressed independent of each other.


CONCLUSIONS
Our results suggest that miRNA expression profiles may not predict intrinsic hormone-sensitive environment of PCa cells. More importantly, our data indicate the possibility of additional novel mechanisms for intronic miRNA processing in PCa cells.
Sikand
Kavleen
K
Center for Gene Regulation in Health and Disease, Cleveland State University, 2121 Euclid Avenue, Cleveland, OH 44115, USA. k.sikand@csuohio.edu
Slane
Stephen D
SD
Shukla
Girish C
GC
Clin Cancer Res. 2008 May 1;14(9):2690-5
18451233
Trends Pharmacol Sci. 2008 Jan;29(1):12-5
18068232
Mamm Genome. 2006 Aug;17(8):833-40
16897339
CA Cancer J Clin. 2008 Mar-Apr;58(2):71-96
18287387
Nature. 2008 Jan 10;451(7175):147-52
18185580
Nat Rev Cancer. 2006 Nov;6(11):857-66
17060945
Nat Rev Cancer. 2001 Oct;1(1):34-45
11900250
Leukemia. 2005 Nov;19(11):2013-6
16167061
Trends Cell Biol. 2007 Mar;17(3):118-26
17197185
Nature. 2005 Feb 17;433(7027):769-73
15685193
BMC Genomics. 2007 Jun 12;8:166
17565689
Mol Cell Biol. 2006 Nov;26(21):8191-201
16940181
Nat Rev Genet. 2004 May;5(5):396-400
15143321
Mol Cell Biol. 2008 Apr;28(7):2167-74
18212054
Nat Immunol. 2008 Apr;9(4):405-14
18327259
Nature. 2005 Jun 9;435(7043):828-33
15944707
Blood. 2008 Jan 15;111(2):588-95
17906079
Cell Res. 2008 May;18(5):549-57
18379589
PLoS One. 2008 May 21;3(5):e2236
18493594
Leukemia. 2008 Jun;22(6):1274-8
17989710
Nucleic Acids Symp Ser (Oxf). 2005;(49):341-2
17150773
J Biol Chem. 2007 Aug 10;282(32):23716-24
17569667
Cancer Res. 1983 Apr;43(4):1809-18
6831420
Anal Biochem. 2006 Oct 15;357(2):299-301
16930526
Cancer Cell. 2008 Mar;13(3):272-86
18328430
Cancer Res. 2007 Sep 15;67(18):8699-707
17875710
Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2257-61
16461460
Nat Rev Cancer. 2006 Apr;6(4):259-69
16557279
Oncogene. 2008 Mar 13;27(12):1788-93
17891175
Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2889-94
18287052
Oncology. 2007;72(5-6):397-402
18196926
Urol Oncol. 2007 Sep-Oct;25(5):387-92
17826655
Nucleic Acids Res. 2008 Jan;36(Database issue):D154-8
17991681
Cancer Res. 2006 Sep 15;66(18):9090-8
16982751
Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19983-8
18056640
Genome Res. 2004 Oct;14(10A):1902-10
15364901
Mol Cancer. 2006 Jun 19;5:24
16784538
Anticancer Res. 2008 Mar-Apr;28(2A):907-11
18507035
Cancer Res. 2008 Jul 15;68(14):5540-5
18632605
Cancer Res. 2004 May 1;64(9):3087-95
15126345
Methods. 2008 Jan;44(1):31-8
18158130
RNA. 2005 Mar;11(3):241-7
15701730
RNA. 2008 Mar;14(3):417-24
18174313
Nature. 2005 Jun 9;435(7043):839-43
15944709
Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3687-92
16505370
Cancer Res. 2007 Jul 1;67(13):6130-5
17616669
Cancer Metastasis Rev. 1998-1999;17(4):361-71
10453280
DNA Cell Biol. 2007 Apr;26(4):209-18
17465887
N Engl J Med. 2003 Jul 24;349(4):366-81
12878745
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13021-6
18755890
J Clin Pathol. 2004 Oct;57(10):1057-62
15452160
Cancer Res. 2005 Nov 1;65(21):9628-32
16266980
Cancer Res. 2008 Mar 1;68(5):1362-8
18316599
Nucleic Acids Res. 2008 Sep;36(16):5391-404
18701644
Proc Natl Acad Sci U S A. 2008 May 13;105(19):7004-9
18458333
Dev Biol. 2007 Oct 15;310(2):442-53
17765889
JAMA. 2008 Jan 30;299(4):425-36
18230780
Nat Rev Cancer. 2002 May;2(5):389-96
12044015
Clin Cancer Res. 1997 Dec;3(12 Pt 1):2493-500
9815652
Mol Cell. 2004 Dec 22;16(6):861-5
15610730
Cell. 2004 Jan 23;116(2):281-97
14744438
Nature. 2008 Sep 4;455(7209):64-71
18668037
Annu Rev Genomics Hum Genet. 2007;8:279-98
17506659
Urol Oncol. 2010 Jan-Feb;28(1):39-48
18799331
Int J Cancer. 2008 Jul 15;123(2):372-379
18449891
Nucleic Acids Res. 2005 Sep 28;33(17):5394-403
16192569