title: [Identification of differentially expressed genes in rat asthma model by suppression subtractive hybridization technology].
abstract: OBJECTIVE
  To identify differentially expressed genes related to asthma by using a rat model.
  
  
  METHODS
  Total RNA extracted from the asthmatic rats was taken as the tester and the total RNA from the control rats as the driver. Suppression subtractive hybridization (SSH) was used to isolate the cDNA fragments of differentially expressed genes. The products of SSH were inserted into pGEM-T Easy vector to establish the subtractive library. The library was amplified through E.coli transformation and positive clones of the transformants were screened. The white clones in selective medium from cDNA library were isolated and digested by EcoR I restriction endonuclease. Thirty-six positive clones were chosen randomly and sequenced. Nucleic acid similarity was subsequently analyzed by comparing with the data from GenBank (NCBI).
  
  
  RESULTS
  There were more than 300 white clones in the cDNA library. The clones were sequenced and similarity search (http://www.ncbi.hlm.nih.gov/BLAST) revealed 4 known genes, 2 ESTs without homologous genes and 3 potential new gene fragments.
  
  
  CONCLUSIONS
  The forward-subtracted cDNA library for differentially expressed in the lung of asthmatic rats has been successfully constructed and the interesting candidate genes related to asthma have been identified.
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Bo
authors.initials: B
authors.lastname: ZHONG
authors.forename: Hui-lian
authors.initials: HL
authors.lastname: WANG
authors.forename: Shahzad
authors.initials: S
authors.lastname: MUHAMMAD
authors.forename: Qi-lan
authors.initials: QL
authors.lastname: NING
authors.forename: Yan
authors.initials: Y
authors.lastname: HAN
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: YANG
authors.forename: Fu-jun
authors.initials: FJ
authors.lastname: ZHANG
authors.forename: She-min
authors.initials: SM
authors.lastname: LU
mesh_terms: D000818:Animals; D001249:Asthma / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D018076:DNA, Complementary