title: Bacillus thuringiensis serovar mogi (flagellar serotype 3a3b3d), a novel serogroup with a mosquitocidal activity.
abstract: The flagellated vegetative cells of the Bacillus thuringiensis strain K4 were agglutinated with the H3 reference antiserum and further, agglutinated with 3b and 3d monospecific factor sera but non-reactive for 3c and 3e factor sera. This creates a new serogroup with flagellar antigenic structure of 3a3b3d: B. thuringiensis serovar mogi. The strain K4 showed high activity against dipteran larvae, Anopheles sinensis and Culex pipienspallens while no lepidopteran toxicity. It produced ovoidal parasporal inclusions (crystals) whose SDS-PAGE protein profile consisted of several bands ranging from 75 to 30kDa. Through the protein identification by nano-LC-ESI-IT MS analysis, the putative peptides of Cry19Ba, Cry40ORF2, Cry27Aa and Cry20Aa were detected.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jong Yul
authors.initials: JY
authors.lastname: Roh
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Dae Weon
authors.initials: DW
authors.lastname: Lee
authors.forename: Xueying
authors.initials: X
authors.lastname: Tao
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Shim
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Jong Bok
authors.initials: JB
authors.lastname: Seo
authors.forename: Michio
authors.initials: M
authors.lastname: Ohba
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizuki
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
mesh_terms: D000818:Animals; D000852:Anopheles / Q000382:microbiology*; D001413:Bacillus thuringiensis / Q000145:classification / Q000378:metabolism / Q000502:physiology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D003465:Culex / Q000382:microbiology*; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D009032:Mosquito Control; D012703:Serotyping
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis