title: Isolation and expression analysis of a novel major latex-like protein (MLP151) gene from Panax ginseng.
abstract: This is the first reports on isolation and expression analysis of a major latex-like protein (MLP151) gene in Panax ginseng C.A. Meyer. A full-length cDNA of MLP151 was 850 bp and contained a 456 bp open reading frame encoding a polypeptide of 151 amino acids. A theoretical pI value of MLP151 was 4.86 and calculated molecular weight was about 16.87 kDa. The MLP homolog proteins are found in various plants and the neighbor-joining analysis revealed that MLP151 has the closest distance with Sn-1 (bell pepper, MLP homolog gene). We analyzed the expression of MLP151 in different levels in various organs of ginseng and plantlet. In the result, the gene was low expressed in plantlet. We treated the ginseng plantlets with nine kinds of different stresses and analyzed the expression profile of MLP151. Transcript levels were significantly induced by stress treatment of light and mannitol, whereas transcript levels were drastically decreased in dark, H(2)O(2), salicylic acid and wounding samples.
authors.affiliation: Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Kiheung-gu Yongin, Kyunggi-do 446-701, South Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Sun
authors.forename: Myung-Kyum
authors.initials: MK
authors.lastname: Kim
authors.forename: Rama Krishna
authors.initials: RK
authors.lastname: Pulla
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D005894:Panax / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D018076:DNA, Complementary; C000598969:MLP151 protein, Panax ginseng; D010940:Plant Proteins
references.citation: Plant Mol Biol. 1992 Nov;20(4):749-52
references.pmid: 1450390
references.citation: Plant Physiol Biochem. 2007 Jan;45(1):33-8
references.pmid: 17258907
references.citation: Plant Physiol. 1993 Oct;103(2):315-321
references.pmid: 12231938
references.citation: J Allergy Clin Immunol. 2007 Sep;120(3):518-25
references.pmid: 17689599
references.citation: Proteomics. 2003 Dec;3(12):2351-67
references.pmid: 14673786
references.citation: Plant Mol Biol. 1990 Dec;15(6):951-3
references.pmid: 2103486
references.citation: Plant Cell. 1989 Jan;1(1):25-35
references.pmid: 2535464
references.citation: Plant Physiol. 2002 Apr;128(4):1291-302
references.pmid: 11950978
references.citation: FEBS Lett. 1997 Apr 28;407(2):207-10
references.pmid: 9166900
references.citation: Science. 1990 Nov 16;250(4983):1004-6
references.pmid: 17746926
references.citation: Science. 1993 Dec 17;262(5141):1883-6
references.pmid: 8266079
references.citation: Gene. 1994 Feb 25;139(2):207-9
references.pmid: 8112605
references.citation: Plant Mol Biol. 1999 Sep;41(2):217-31
references.pmid: 10579489
references.citation: Planta. 1996;199(3):413-5
references.pmid: 8771801
references.citation: Plant Mol Biol. 1995 Sep;28(6):1011-25
references.pmid: 7548820
references.citation: Plant Cell. 1994 Jan;6(1):65-74
references.pmid: 12244221
references.citation: Science. 1990 Nov 16;250(4983):1002-4
references.pmid: 17746925
references.citation: Plant Cell Physiol. 2004 Apr;45(4):357-68
references.pmid: 15111710
references.citation: Plant Physiol. 1985 Oct;79(2):499-504
references.pmid: 16664439
references.citation: Planta. 1990 Mar;180(4):487-91
references.pmid: 24202092
references.citation: Fundam Clin Pharmacol. 2005 Aug;19(4):405-9
references.pmid: 16011726
references.citation: Plant Cell. 1990 Jul;2(7):673-84
references.pmid: 2152343
references.citation: Plant Mol Biol. 1998 Dec;38(6):1243-6
references.pmid: 9869429
references.citation: Plant Mol Biol. 1997 Jan;33(2):313-22
references.pmid: 9037149
references.citation: Plant Mol Biol. 1999 Feb;39(3):629-36
references.pmid: 10092188