G-quadruplex DNA bound by a synthetic ligand is highly dynamic.
Using single molecule fluorescence resonance energy transfer, we investigated the interaction between a quadruplex-binding ligand and the human telomeric G-quadruplex. The binding of quinolinecarboxamide macrocycle to telomeric DNA was essentially irreversible and selectively induced and favored one quadruplex conformation. The ligand-quadruplex complex displayed intramolecular dynamics including quadruplex folding and unfolding in the absence of ligand association and dissociation. We report that the G-quadruplex can be stabilized without preventing the intrinsic intramolecular dynamics of telomeric DNA.
Jena
Prakrit V
PV
Department of Physics, Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Shirude
Pravin S
PS
Okumus
Burak
B
Laxmi-Reddy
Katta
K
Godde
Frédéric
F
Huc
Ivan
I
Balasubramanian
Shankar
S
Ha
Taekjip
T
D000818:Animals; D001360:Avidin / Q000378:metabolism; D001483:Base Sequence; D001710:Biotin / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D054856:G-Quadruplexes; D006801:Humans; D008024:Ligands; D011791:Quartz / Q000737:chemistry; D016615:Telomere / Q000235:genetics
D008024:Ligands; C093483:neutravidin; D001360:Avidin; D011791:Quartz; D001710:Biotin; D004247:DNA
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