title: [Therapeutic effect of allogenic bone marrow transplantation in acute TNBS-induced colitis].
abstract: OBJECTIVE
  Bone marrow-derived cells (BMDC) contribute to tissue maintenance under many kinds of pathologic conditions. We carried out a study to see how BMDC play a role in the treatment of experimental murine colitis.
  
  
  METHODS
  We divided the animals into 3 groups and treated them with 50% ethanol (control group), 2,4,6-trinitrobenzene sulfinic acid colitis (TNBS group), and TNBS+bone marrow transplant (BMT group). To induce colitis, TNBS (5.0 mg/mouse) dissolved in 50% ethanol was injected into anus weekly for two weeks. Bone marrow transplantations were performed using bone marrow of male transgenic mouse (donor) with green fluorescence protein (GFP) into female wild type mouse (recipient) three weeks before TNBS instillation. All animals were sacrificed, and colons were extracted one week after the last TNBS instillation. We measured microscopic scores of mucosal injury and investigated the GFP expression for bone marrow engraftment. The immunostaining of vimentin and alpha-smooth muscle actin (alpha-SMA) for myofibroblasts was performed.
  
  
  RESULTS
  The score of mucosal injury in the TNBS group was much more severe than those in control, and reduced significantly by BMT (p<0.05). GFP-positive cells were almost deposited in pericryptal niche of BMT group but not at all in both control and TNBS group. Most of myofibroblasts stained with both vimentin and SMA also infiltrated into pericryptal niche. But, the number of myofibroblasts stained with vimentin and SMA in both control and TNBS group was smaller than that in BMT group.
  
  
  CONCLUSIONS
  BMDC deposited on pericryptal niche might have a significant role in repairing acute experimental murine colitis.
authors.affiliation: Department of Internal Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Lee So
authors.initials: LS
authors.lastname: Maeng
authors.forename: Eun Duck
authors.initials: ED
authors.lastname: Chang
authors.forename: Hiun Suk
authors.initials: HS
authors.lastname: Chae
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Jeong Yo
authors.initials: JY
authors.lastname: Min
authors.forename: Hye Sook
authors.initials: HS
authors.lastname: Sohn
authors.forename: Sang Young
authors.initials: SY
authors.lastname: Rho
authors.forename: Hyung Keun
authors.initials: HK
authors.lastname: Kim
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Cho
authors.forename: Kyu Yong
authors.initials: KY
authors.lastname: Choi
authors.forename: Hae Kyung
authors.initials: HK
authors.lastname: Lee
mesh_terms: D000199:Actins / Q000378:metabolism; D000208:Acute Disease; D000818:Animals; D016026:Bone Marrow Transplantation; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D007413:Intestinal Mucosa / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D014184:Transplantation, Homologous; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity*; D014746:Vimentin / Q000378:metabolism
chemical_list: D000199:Actins; D014746:Vimentin; D014302:Trinitrobenzenesulfonic Acid