title: Amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation for hydrolysis of Cassava starch.
abstract: The optimization of process parameters for high amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation was carried out using central composite design. Finally, the optimal parameters obtained with the response surface methodology (RSM) were moisture 610.0 ml/kg, inoculum 30.0 ml (OD(600 nm) = 20.0)/kg, the amount ratio of wheat bran to rice husk 0.42, cassava starch concentration 20.0 g/kg, temperature 28 degrees C, and natural pH. Under the optimized conditions, 4,296 U/g of dry substrate of amylase activity was reached in the solid-state fermentation culture of the yeast strain A11 within 160 h, whereas the predicted maximum amylase activity of 4,222 U/g of dry substrate of amylase activity was derived from the RSM regression. It was found that cassava starch can be actively converted into monosaccharides and oligosaccharides by the crude amylase.
authors.affiliation: Key Laboratory of Marine Genetics and Gene Resource Exploitation (Ministry of Education), Ocean University of China, Yushan Road, No.5, Qingdao, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chi
authors.forename: Mei
authors.initials: M
authors.lastname: Li
mesh_terms: D000681:Amylases / Q000096:biosynthesis*; D000704:Analysis of Variance; D005285:Fermentation; D006868:Hydrolysis; D002365:Manihot / Q000737:chemistry*; D015203:Reproducibility of Results; D012440:Saccharomyces / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*
chemical_list: D013213:Starch; D000681:Amylases