title: Vertical hydrodynamic focusing in glass microchannels.
abstract: Vertical hydrodynamic focusing in microfluidic devices is investigated through simulation and through direct experimental verification using a confocal microscope and a novel form of stroboscopic imaging. Optimization for microfluidic cytometry of biological cells is examined. By combining multiple crossing junctions, it is possible to confine cells to a single analytic layer of interest. Subtractive flows are investigated as a means to move the analysis layer vertically in the channel and to correct the flatness of this layer. The simulation software (ADINA and Coventor) is shown to accurately capture the complex dependencies of the layer interfaces, which vary strongly with channel geometry and relative flow rates.
authors.forename: Tony A
authors.initials: TA
authors.lastname: Lin
authors.forename: A E
authors.initials: AE
authors.lastname: Hosoi
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Ehrlich
references.citation: Anal Chem. 2000 Jul 15;72(14):3388-91
references.pmid: 10939418
references.citation: J Am Chem Soc. 2004 Mar 10;126(9):2674-5
references.pmid: 14995164
references.citation: J Chromatogr A. 2006 Apr 14;1111(2):206-13
references.pmid: 16569579
references.citation: Nat Biotechnol. 2005 Jan;23(1):83-7
references.pmid: 15608628
references.citation: Lab Chip. 2009 Jan 21;9(2):305-10
references.pmid: 19107289