Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments.
Thermal convection and high intensity solvent resonances can significantly hamper diffusion estimates in pulsed gradient spin-echo nuclear magnetic resonance diffusion experiments on biomolecule samples. To overcome these two problems, a new double functional NMR diffusion sequence, double echo PGSTE-WATERGATE, is presented. The new sequence provides excellent convection compensation and solvent suppression (with a suppression factor in excess of at least 10(5) in a single scan) in biomolecular NMR diffusion experiments. Due to its stimulated echo nature, the new sequence is much less susceptible to spin-spin relaxation than Hahn spin-echo based sequences. Furthermore, the new sequence is not susceptible to spin diffusion due to the application of bipolar pulsed gradients. The new sequence is also much easier to set up compared to previously developed stimulated echo based convection compensation and solvent suppression sequence. The utility of the new sequence is demonstrated on an aqueous lysozyme sample.
Zheng
Gang
G
Nanoscale Organisation and Dynamics Group, College of Health and Science, University of Western Sydney, Penrith South DC, NSW, 1797, Australia.
Price
William S
WS
D004058:Diffusion; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D011506:Proteins; D012997:Solvents
J Magn Reson. 2008 Sep;194(1):108-14
18619877
J Magn Reson. 1999 Aug;139(2):334-41
10423370
J Magn Reson. 2000 Oct;146(2):283-9
11001844
J Magn Reson. 2004 Oct;170(2):322-8
15388096
J Magn Reson. 2001 May;150(1):35-8
11330980
J Magn Reson B. 1994 May;104(1):1-10
8025810
J Magn Reson. 2004 Apr;167(2):328-33
15040990
J Magn Reson. 2001 Jun;150(2):126-31
11384170
J Magn Reson. 2005 Dec;177(2):203-11
16140552
J Magn Reson. 2008 Mar;191(1):159-63
18086542
J Biomol NMR. 2004 Jul;29(3):299-308
15213428
Anal Bioanal Chem. 2007 May;388(1):173-7
17393152
J Magn Reson. 2000 Jul;145(1):125-31
10873503
J Magn Reson. 2000 Feb;142(2):323-5
10648149
Anal Chem. 2001 Aug 1;73(15):3528-34
11510814
J Magn Reson. 2005 Jun;174(2):229-36
15862239
J Magn Reson. 2004 Jul;169(1):92-101
15183361
J Magn Reson. 1998 Mar;131(1):126-30
9533914
J Magn Reson. 2006 Jun;180(2):266-73
16563821
J Magn Reson. 2001 Nov;153(1):48-55
11700080