Diversity and evolution of MHII beta genes in a non-model percid species -- the Eurasian perch (Perca fluviatilis L.).
This study provides the first investigation of the diversity, structure, and molecular evolution of MHII beta genes in a non-model percid species -- the Eurasian perch (Perca fluviatilis L.). PCR primers developed here were highly specific, and documented a high diversity of the MHII beta1 domain in perch. Our results suggest a minimum of eight MHII beta loci in this species -- a finding congruent with several studies suggesting that many Euteleostei posses multiple MHII beta loci. As for other vertebrates, both positive selection and gene-conversion contribute to the reported high allelic diversity. Similarly, the MHII beta1 domain in perch exhibits a characteristic MHC fold known from other vertebrates. In addition, our results suggest some teleost specific differences of the MHII beta1 domain, including: differences in chemical properties of specific amino acids in the beta1 domain, the absence of the tetrapod specific glycolisation signal, and differences in the positions of some of the positively selected codons in the MHII beta1 domain, which are presumably involved in antigen binding. Future studies should investigate the teleost MHII beta genes in more details in order to confirm the suggested differences, and to determine the extent to which these differences prevail in different teleost lineages.
Michel
Christian
C
Program MGU, Man-Society-Environment, Department of Environmental Sciences, University of Basel, Basel, Switzerland.
Bernatchez
Louis
L
Behrmann-Godel
Jasminca
J
D000818:Animals; D001665:Binding Sites / Q000502:physiology; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics* / Q000276:immunology; D005785:Gene Conversion / Q000502:physiology*; D006031:Glycosylation; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D010471:Perches / Q000235:genetics* / Q000276:immunology; D017434:Protein Structure, Tertiary / Q000502:physiology; D040641:Quantitative Trait Loci / Q000235:genetics* / Q000276:immunology
D029941:Fish Proteins; D000949:Histocompatibility Antigens Class II