Histone deacetylase inhibitor trichostatin A modulates cell cycles in A2780 human ovarian cancer cell lines.
OBJECTIVE
To analyze the effect and mechanism of TSA on cell cycles in human ovarian cancer cells.


METHODS
cells were cultured in RPMI 1640 supplemented. Flow cytometry analysis and RT-PCR were used to examine the distribution of cell cycles and the level of P21(WAF/CIPI) mRNA.


RESULTS
TSA induced increase of the G2/M phase accompanied by decrease of the S phase and enhanced level of P21(WAF/CIPI) mRNA in a concentration and time-dependent manner in A2780 cells.


CONCLUSIONS
Trichostatin A affects the activity of cyclin-dependent kinase through increased expression of P21(WAF/CIPI) mRNA. TSA causes A2780 cell blockage in the G2/M phase and inhibits cell proliferation of A2780 cells. The minimum level of active TSA is 100 nM and the minimum time is 12 hours. The effect relies on time and concentration.
Ma
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Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China. maxiaoxin666@yahoo.com.cn
Jin
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Miao
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Li
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S
He
Y Q
YQ
Lv
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HW
D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D050759:Cyclin-Dependent Kinase Inhibitor p21; D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A