title: Depressive-like parameters in sepsis survivor rats.
abstract: The inflammatory and immune responses evoked in sepsis may create not only an acute brain dysfunction, which occurs in the majority of septic patients, but also long-term deficits such as memory impairment. In this context, we evaluated depressive-like parameters in sepsis survivor rats. For this purpose, male Wistar rats, weighing 300-350 g, underwent cecal ligation and perforation (CLP) (sepsis group) followed by "basic support", or were sham-operated (control group). After 3 days of the sepsis procedure, the animals were treated with imipramine at 10 mg/kg or saline during 14 days (days 3-17). The consumption of sweet food was measured for 7 days (days 10-17) and the body weight was measured before CLP, 10, and 17 days after CLP. Seventeen days after sepsis (immediately after sweet food consumption measurement), the animals were anesthetized and blood was withdrawn for the analyses of corticosterone and adrenocorticotropic hormone (ACTH) levels, and immediately killed by decapitation. The adrenal gland and hippocampus were immediately isolated and weighed, and the hippocampus was utilized for determining brain-derived neurotrophic factor (BDNF) levels. It was observed that animals subjected to CLP presented decreased sucrose intake. Septic rats did not increase body weight and presented an increase in the weight of adrenal gland. Both corticosterone and ACTH levels were increased, while hippocampus weight and BDNF levels in the hippocampus decreased. The treatment with imipramine reversed all the parameters described above. Our results supported the hypothesis that rats that survive sepsis show depressive-like behavior, alterations in the hypothalamus-pituitary-adrenal axis, and decreased BDNF levels in the hippocampus.
authors.affiliation: Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
authors.forename: Clarissa M
authors.initials: CM
authors.lastname: Comim
authors.forename: Omar J
authors.initials: OJ
authors.lastname: Cassol
authors.forename: Leandra C
authors.initials: LC
authors.lastname: Constantino
authors.forename: Fabrícia
authors.initials: F
authors.lastname: Petronilho
authors.forename: Larissa S
authors.initials: LS
authors.lastname: Constantino
authors.forename: Laura
authors.initials: L
authors.lastname: Stertz
authors.forename: Flávio
authors.initials: F
authors.lastname: Kapczinski
authors.forename: Tatiana
authors.initials: T
authors.lastname: Barichello
authors.forename: João
authors.initials: J
authors.lastname: Quevedo
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D003863:Depression / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D005518:Food Preferences / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007099:Imipramine / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000150:complications*
chemical_list: D000929:Antidepressive Agents, Tricyclic; D019208:Brain-Derived Neurotrophic Factor; D000324:Adrenocorticotropic Hormone; D007099:Imipramine; D003345:Corticosterone
references.citation: Neuroscience. 2006 May 12;139(2):779-89
references.pmid: 16457953
references.citation: Clin Invest Med. 1990 Dec;13(6):297-304
references.pmid: 2078909
references.citation: Curr Opin Pharmacol. 2007 Feb;7(1):18-21
references.pmid: 17049922
references.citation: Nat Clin Pract Endocrinol Metab. 2008 Sep;4(9):496-505
references.pmid: 18695699
references.citation: Prog Neurobiol. 2005 Jun;76(2):99-125
references.pmid: 16099088
references.citation: Pharmacol Biochem Behav. 2006 Dec;85(4):842-9
references.pmid: 17204313
references.citation: Neurochem Int. 2003 Jan;42(2):107-14
references.pmid: 12421590
references.citation: Mitochondrion. 2008 Sep;8(4):313-8
references.pmid: 18657632
references.citation: Neuroendocrinology. 2001 Sep;74(3):148-59
references.pmid: 11528216
references.citation: Psychopharmacology (Berl). 2006 Jul;186(4):601-11
references.pmid: 16758243
references.citation: Brain Res Brain Res Rev. 2004 May;45(2):104-14
references.pmid: 15145621
references.citation: Behav Pharmacol. 2005 May;16(3):171-80
references.pmid: 15864072
references.citation: Front Neuroendocrinol. 2004 Jul;25(2):77-107
references.pmid: 15571756
references.citation: Crit Care Med. 2001 Jul;29(7):1303-10
references.pmid: 11445675
references.citation: Psychopharmacology (Berl). 1998 Apr;136(3):272-83
references.pmid: 9566813
references.citation: Crit Rev Neurobiol. 1995;9(2-3):189-205
references.pmid: 8581983
references.citation: Nature. 2008 Oct 16;455(7215):894-902
references.pmid: 18923511
references.citation: Intensive Care Med. 2003 Oct;29(10):1782-9
references.pmid: 12783160
references.citation: Brain Behav Immun. 2006 Jan;20(1):1-8
references.pmid: 16140497
references.citation: Brain Res. 2001 Apr 27;899(1-2):227-39
references.pmid: 11311884
references.citation: Crit Care Med. 2007 Sep;35(9 Suppl):S596-601
references.pmid: 17713415
references.citation: Crit Care Med. 2003 Apr;31(4):1226-34
references.pmid: 12682497
references.citation: Psychosom Med. 2005 May-Jun;67 Suppl 1:S26-8
references.pmid: 15953796
references.citation: Biol Psychiatry. 2006 Jun 15;59(12):1116-27
references.pmid: 16631126
references.citation: Neurochem Res. 2008 Nov;33(11):2171-7
references.pmid: 18461451
references.citation: J Neurosci. 1997 Apr 1;17(7):2295-313
references.pmid: 9065491
references.citation: Biol Psychiatry. 2002 Sep 15;52(6):503-28
references.pmid: 12361666
references.citation: Curr Neurovasc Res. 2008 Nov;5(4):207-13
references.pmid: 18991655
references.citation: Immunol Today. 2000 Jun;21(6):281-9
references.pmid: 10825740
references.citation: Neurosci Biobehav Rev. 1981 Summer;5(2):231-46
references.pmid: 7022272
references.citation: Physiol Behav. 1988;44(4-5):483-90
references.pmid: 2853383
references.citation: Drug News Perspect. 2006 Dec;19(10):603-8
references.pmid: 17299602
references.citation: Psychiatry Res. 2002 Mar 15;109(2):143-8
references.pmid: 11927139
references.citation: Neurochem Int. 1997 Apr-May;30(4-5):427-31
references.pmid: 9106257
references.citation: Brain Res. 2008 Feb 27;1196:41-52
references.pmid: 18234161
references.citation: Intensive Care Med. 2007 Dec;33(12):2165-7
references.pmid: 17668182
references.citation: Neuropsychopharmacology. 2004 Apr;29(4):795-8
references.pmid: 14735133
references.citation: Arch Gen Psychiatry. 1994 Jan;51(1):8-19
references.pmid: 8279933
references.citation: Curr Top Microbiol Immunol. 1996;216:39-81
references.pmid: 8791735
references.citation: Neuroimmunomodulation. 1994 Jul-Aug;1(4):251-8
references.pmid: 7489340
references.citation: Anaesthesia. 2001 Jan;56(1):9-14
references.pmid: 11167429
references.citation: Ann N Y Acad Sci. 2000;917:876-93
references.pmid: 11268419
references.citation: Mol Cell Neurosci. 2004 Nov;27(3):280-95
references.pmid: 15519243
references.citation: Neuroscience. 2002;112(2):309-18
references.pmid: 12044449
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2009 Apr 30;33(3):450-5
references.pmid: 19439250
references.citation: J Neuroimmunol. 2006 Mar;172(1-2):38-58
references.pmid: 16375977
references.citation: Physiol Rev. 2007 Jul;87(3):873-904
references.pmid: 17615391
references.citation: Exp Neurol. 2007 Apr;204(2):733-40
references.pmid: 17306796
references.citation: Vitam Horm. 1983;40:145-74
references.pmid: 6673332
references.citation: Arch Gen Psychiatry. 1997 Jul;54(7):597-606
references.pmid: 9236543
references.citation: Annu Rev Neurosci. 1996;19:289-317
references.pmid: 8833445
references.citation: Crit Care Med. 1990 Aug;18(8):801-6
references.pmid: 2379391
references.citation: Ann N Y Acad Sci. 2002 Jun;966:20-7
references.pmid: 12114255
references.citation: Intensive Care Med. 2008 Sep;34(9):1724-31
references.pmid: 18542919
references.citation: Psychopharmacology (Berl). 1987;93(3):358-64
references.pmid: 3124165
references.citation: Neurosci Biobehav Rev. 2005 Feb;29(1):3-38
references.pmid: 15652252
references.citation: J Affect Disord. 2007 Aug;101(1-3):239-44
references.pmid: 17173978