[Identification of Leishmania species isolated in human cases in Mato Grosso do Sul, by means of the polymerase chain reaction].
Leishmaniases are endemic zoonoses in the State of Mato Grosso do Sul. Their etiological agents in this region of Brazil are Leishmania (Leishmania) chagasi, Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis. The polymerase chain reaction (PCR) is a tool with high specificity and sensitivity for identifying Leishmania species. This study examined 39 cryopreserved isolates of Leishmania that had been collected by bone marrow aspiration and/or lesion biopsy, depending on the clinical suspicion. The isolates were subjected to DNA extraction and PCR using the following primers: RV1/RV2 for identifying Leishmania (Leishmania) chagasi, a1/a2 for Leishmania (Leishmania) amazonensis and b1/b2 for Leishmania (Viannia) braziliensis.Leishmania (Leishmania) chagasi was the only species identified in the 37 cases of visceral leishmaniasis.Leishmania (Leishmania) amazonensis was identified in two isolates from patients with a diagnosis of cutaneous leishmaniasis. The results obtained confirm that it is possible to use these three pairs of primers as a tool for characterizing Leishmania isolates.
Lima Junior
Manoel Sebastião da Costa
MS
Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS.
Andreotti
Renato
R
Dorval
Maria Elizabeth Moraes Cavalheiros
ME
Oshiro
Elisa Teruya
ET
Oliveira
Alessandra Gutierrez de
AG
Matos
Maria de Fatima Cepa
Mde F
D000818:Animals; D001938:Brazil; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D017931:DNA Primers; D016054:DNA, Protozoan