title: Forward and reverse genetics to identify genes involved in the age-related resistance response in Arabidopsis thaliana.
abstract: SUMMARY Age-related resistance (ARR) occurs in numerous plant species, often resulting in increased disease resistance as plants mature. ARR in Arabidopsis to Pseudomonas syringae pv. tomato is associated with intercellular salicylic acid (SA) accumulation and the transition to flowering. Forward and reverse genetic screens were performed to identify genes required for ARR and to investigate the mechanism of the ARR response. Infiltration of SA into the intercellular space of the ARR-defective mutant iap1-1 (important for the ARR pathway) partially restored ARR function. Inter- and intracellular SA accumulation was reduced in the mutant iap1-1 compared with the wild-type, and the SA regulatory gene EDS1 was also required for ARR. Combining microarray analysis with reverse genetics using T-DNA insertion lines, four additional ARR genes were identified as contributing to ARR: two plant-specific transcription factors of the NAC family [ANAC055 (At3g15500) and ANAC092 (At5g39610)], a UDP-glucose glucosyltransferase [UGT85A1 (At1g22400)] and a cytidine deaminase [CDA1 (At2g19570)]. These four genes and IAP1 are also required for ARR to Hyaloperonospora parasitica. IAP1 encodes a key component of ARR that acts upstream of SA accumulation and possibly downstream of UGT85A1, CDA1 and the two NAC transcription factors (ANAC055, ANAC092).
authors.affiliation: Department of Biology, McMaster University, Hamilton, ON, Canada, L8S 4K1.
authors.forename: Jessie L
authors.initials: JL
authors.lastname: Carviel
authors.forename: Fadi
authors.initials: F
authors.lastname: Al-Daoud
authors.forename: Melody
authors.initials: M
authors.lastname: Neumann
authors.forename: Asif
authors.initials: A
authors.lastname: Mohammad
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Provart
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Moeder
authors.forename: Keiko
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Robin K
authors.initials: RK
authors.lastname: Cameron
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000276:immunology / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005821:Genetic Techniques; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D044742:Peronospora / Q000187:drug effects / Q000254:growth & development; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D044224:Pseudomonas syringae / Q000187:drug effects; D020156:Salicylic Acid / Q000378:metabolism / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D020156:Salicylic Acid
references.citation: Cell Res. 2008 Jul;18(7):756-67
references.pmid: 18427573
references.citation: Plant Cell. 1994 Jul;6(7):959-965
references.pmid: 12244262
references.citation: Plant Physiol. 1992 Sep;100(1):457-63
references.pmid: 16652983
references.citation: Plant Mol Biol. 2002 Sep;50(2):237-48
references.pmid: 12175016
references.citation: Curr Opin Plant Biol. 2005 Aug;8(4):383-9
references.pmid: 15939664
references.citation: J Biol Chem. 2001 Feb 9;276(6):4338-43
references.pmid: 11042215
references.citation: Eur J Biochem. 1999 Aug;263(3):896-903
references.pmid: 10469156
references.citation: Plant Physiol. 1994 Apr;104(4):1109-1112
references.pmid: 12232151
references.citation: Nature. 2001 Nov 29;414(6863):562-5
references.pmid: 11734859
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3292-7
references.pmid: 10077677
references.citation: Mol Plant Pathol. 2005 Nov 1;6(6):629-39
references.pmid: 20565685
references.citation: Trends Plant Sci. 2005 Feb;10(2):71-8
references.pmid: 15708344
references.citation: Genomics. 2007 Jul;90(1):143-53
references.pmid: 17498920
references.citation: J Biol Chem. 2005 May 6;280(18):17873-9
references.pmid: 15710611
references.citation: Plant Cell. 2004 Aug;16(8):2117-27
references.pmid: 15258265
references.citation: EMBO J. 2001 Oct 1;20(19):5400-11
references.pmid: 11574472
references.citation: Plant Mol Biol. 2003 Oct;53(3):383-97
references.pmid: 14750526
references.citation: Plant Cell. 2005 Sep;17(9):2601-13
references.pmid: 16040633
references.citation: Plant Cell. 1999 Aug;11(8):1393-404
references.pmid: 10449575
references.citation: Curr Opin Plant Biol. 2000 Aug;3(4):315-9
references.pmid: 10873843
references.citation: Science. 2003 Aug 1;301(5633):653-7
references.pmid: 12893945
references.citation: J Biol Chem. 1999 Dec 10;274(50):35483-91
references.pmid: 10585420
references.citation: Science. 1994 Nov 18;266(5188):1247-50
references.pmid: 17810266
references.citation: Trends Plant Sci. 2005 Feb;10(2):79-87
references.pmid: 15708345
references.citation: Plant J. 2006 May;46(4):601-12
references.pmid: 16640597
references.citation: Plant Cell. 2005 Aug;17(8):2397-412
references.pmid: 16024588
references.citation: Plant Cell. 2002 Feb;14(2):479-90
references.pmid: 11884688
references.citation: Curr Opin Plant Biol. 1998 Aug;1(4):311-5
references.pmid: 10066598
references.citation: Plant J. 2005 Dec;44(6):903-16
references.pmid: 16359384
references.citation: Plant Cell. 2004 Jul;16(7):1938-50
references.pmid: 15208388
references.citation: Plant Cell. 2007 Jun;19(6):2039-52
references.pmid: 17573535
references.citation: Plant Cell. 2004 Sep;16(9):2481-98
references.pmid: 15319476
references.citation: Development. 1999 Apr;126(8):1563-70
references.pmid: 10079219
references.citation: Plant Physiol. 2005 Dec;139(4):1890-901
references.pmid: 16306146
references.citation: New Phytol. 2007;175(3):405-416
references.pmid: 17635216
references.citation: Immunol Rev. 2004 Apr;198:249-66
references.pmid: 15199967
references.citation: Plant J. 1999 Oct;20(2):163-170
references.pmid: 10571876
references.citation: Protein Expr Purif. 1999 Feb;15(1):8-15
references.pmid: 10024464
references.citation: Plant J. 1999 May;18(3):265-76
references.pmid: 10377992
references.citation: Plant Cell. 2004 Dec;16(12):3496-507
references.pmid: 15548740
references.citation: Science. 1993 Aug 6;261(5122):754-6
references.pmid: 17757215
references.citation: Plant Cell. 2003 Jul;15(7):1563-77
references.pmid: 12837947
references.citation: Mol Plant Microbe Interact. 1995 Nov-Dec;8(6):863-70
references.pmid: 8664495
references.citation: Curr Opin Microbiol. 2005 Aug;8(4):399-404
references.pmid: 15996507
references.citation: Plant Cell. 1991 Aug;3(8):809-18
references.pmid: 1820820
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
references.pmid: 11309499
references.citation: FEBS Lett. 1995 Dec 27;377(3):523-9
references.pmid: 8549790
references.citation: Ann Bot. 2007 Oct;100(4):681-97
references.pmid: 17513307
references.citation: Plant J. 2004 Nov;40(4):462-73
references.pmid: 15500463
references.citation: DNA Res. 2003 Dec 31;10(6):239-47
references.pmid: 15029955
references.citation: Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6602-6
references.pmid: 11607555
references.citation: Genes Dev. 2000 Dec 1;14(23):3024-36
references.pmid: 11114891
references.citation: Annu Rev Genet. 2000;34:499-531
references.pmid: 11092837
references.citation: Curr Opin Plant Biol. 2005 Aug;8(4):378-82
references.pmid: 15922650
references.citation: Plant Cell. 2002 Jan;14(1):275-86
references.pmid: 11826312
references.citation: Plant J. 2003 Mar;33(5):887-98
references.pmid: 12609030
references.citation: Cell. 1996 Apr 19;85(2):159-70
references.pmid: 8612269
references.citation: Plant Cell. 1991 Jan;3(1):49-59
references.pmid: 1824334
references.citation: J Plant Growth Regul. 2003 Dec;22(4):289-297
references.pmid: 14676968
references.citation: Mol Plant Microbe Interact. 2006 Jul;19(7):789-800
references.pmid: 16838791
references.citation: Plant Cell. 1997 Jun;9(6):841-57
references.pmid: 9212461
references.citation: Plant J. 2004 Sep;39(5):790-808
references.pmid: 15315639
references.citation: Plant Physiol. 2004 Jun;135(2):1113-28
references.pmid: 15181213