title: Dopamine regulates the expression of the glutamate transporter GLT1 but not GLAST in developing striatal astrocytes.
abstract: Dopamine and L: -glutamate are important signals which guide the development of functional neural circuits within the striatal complex. Disequilibrium of these neurotransmitter systems is believed to be etiological for the genesis of neurological and psychiatric diseases. Since dopamine plays a crucial role for the early transmitter-regulated differentiation of striatal GABAergic neurons, we emphasized that dopaminergic transmission may also be involved in the fine tuning of intra-striatal glutamate action. In this study, we report that dopamine decreases the expression of the glutamate transporter GLT1 but not GLAST in striatal astrocytes by measuring gene and protein expression. Using glutamate-uptake approaches, we demonstrate an increase in glutamate clearance of externally added glutamate in dopamine-treated cultures compared to controls. Our findings imply that dopamine regulates the availability of L: -glutamate in the developing striatum. It is also suggested that the application of dopaminergic drugs can interfere with ontogenetic processes within the striatal complex.
authors.affiliation: Department of Cellular Neurobiology, Eberhard-Karls University of Tübingen, Institute of Anatomy, 72074 Tübingen, Germany.
authors.forename: Veronica I
authors.initials: VI
authors.lastname: Brito
authors.forename: Verena E
authors.initials: VE
authors.lastname: Rozanski
authors.forename: Cordian
authors.initials: C
authors.lastname: Beyer
authors.forename: Eva
authors.initials: E
authors.lastname: Küppers
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D027341:Excitatory Amino Acid Transporter 1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D027342:Excitatory Amino Acid Transporter 2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism* / Q000494:pharmacology; D008657:Metabolic Clearance Rate / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology
chemical_list: D027341:Excitatory Amino Acid Transporter 1; D027342:Excitatory Amino Acid Transporter 2; D012333:RNA, Messenger; C493393:Slc1a3 protein, mouse; D018698:Glutamic Acid; D004298:Dopamine
references.citation: Neuroscience. 1998 Aug;85(4):1183-94
references.pmid: 9681956
references.citation: Neurochem Int. 2008 Jun;52(7):1373-82
references.pmid: 18400334
references.citation: J Neuroendocrinol. 2007 Oct;19(10):819-22
references.pmid: 17850464
references.citation: Trends Neurosci. 1999 Oct;22(10):451-8
references.pmid: 10481192
references.citation: Glia. 1999 Feb 1;25(3):270-81
references.pmid: 9932873
references.citation: Eur J Pharmacol. 2003 Oct 31;479(1-3):237-47
references.pmid: 14612154
references.citation: Dev Neurosci. 1998;20(2-3):229-36
references.pmid: 9691196
references.citation: Glia. 2005 May;50(3):270-5
references.pmid: 15712205
references.citation: Neuroscience. 1996 Sep;74(2):453-60
references.pmid: 8865196
references.citation: Neurochem Int. 1997 Nov;31(5):683-92
references.pmid: 9364454
references.citation: Neurosignals. 2008;16(2-3):112-23
references.pmid: 18253052
references.citation: Cell. 1994 Nov 18;79(4):729-42
references.pmid: 7954836
references.citation: J Neurosci. 2007 Feb 28;27(9):2377-86
references.pmid: 17329435
references.citation: J Neurochem. 2008 Jun 1;105(6):2260-70
references.pmid: 18315563
references.citation: Cell. 1995 Dec 29;83(7):1197-209
references.pmid: 8548806
references.citation: Mol Pharmacol. 1997 Jul;52(1):6-15
references.pmid: 9224806
references.citation: Neuroscience. 1991;41(1):1-24
references.pmid: 1676137
references.citation: Eur J Neurosci. 2008 Dec;28(11):2173-82
references.pmid: 19046364
references.citation: Neurobiol Dis. 2005 Nov;20(2):450-60
references.pmid: 15896973
references.citation: Mol Cell Biol. 2008 May;28(10):3273-80
references.pmid: 18332114
references.citation: Am J Psychiatry. 1992 Apr;149(4):443-54
references.pmid: 1372794
references.citation: Neurotox Res. 2003;5(1-2):1-26
references.pmid: 12832221
references.citation: Brain Res Dev Brain Res. 1996 Jul 20;94(2):242-5
references.pmid: 8836584
references.citation: J Neurosci. 2001 Aug 1;21(15):5546-58
references.pmid: 11466426
references.citation: J Neurosci. 2004 Jul 14;24(28):6301-6
references.pmid: 15254085
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2001 Jan;25(1):5-26
references.pmid: 11263758
references.citation: J Neurochem. 2006 Aug;98(4):1007-18
references.pmid: 16800850
references.citation: Glia. 2004 May;46(3):284-95
references.pmid: 15048851
references.citation: Brain Res. 2003 Jan 10;959(2):251-62
references.pmid: 12493613
references.citation: Psychoneuroendocrinology. 2008 Jul;33(6):832-8
references.pmid: 18472350
references.citation: Neuroscience. 2008 Apr 22;153(1):329-37
references.pmid: 18353560
references.citation: J Recept Signal Transduct Res. 2004 Aug;24(3):165-205
references.pmid: 15521361
references.citation: Brain Res Mol Brain Res. 2005 Jul 29;138(1):1-7
references.pmid: 15896872
references.citation: Brain Res Bull. 2001 Jul 1;55(4):533-40
references.pmid: 11543954
references.citation: Hippocampus. 2004;14(8):975-85
references.pmid: 15390174
references.citation: Brain Res. 1991 May 10;548(1-2):215-21
references.pmid: 1678295
references.citation: Neurosci Lett. 2008 Jan 17;430(3):275-80
references.pmid: 18079058
references.citation: Neurochem Int. 2008 Jan;52(1-2):142-54
references.pmid: 17669556
references.citation: Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8629-34
references.pmid: 10900020
references.citation: Dev Neurosci. 1998;20(2-3):253-62
references.pmid: 9691199
references.citation: Mol Cell Neurosci. 1998 May;11(1-2):9-18
references.pmid: 9608529
references.citation: Neurosci Lett. 1983 Dec 30;43(2-3):191-6
references.pmid: 6143287
references.citation: Neurochem Int. 2000 Aug-Sep;37(2-3):163-70
references.pmid: 10812201
references.citation: J Neurobiol. 1999 Jun 15;39(4):515-26
references.pmid: 10380073
references.citation: Eur J Neurosci. 2004 Sep;20(5):1255-66
references.pmid: 15341597
references.citation: Neurochem Int. 1998 Dec;33(6):479-91
references.pmid: 10098717
references.citation: J Neurosci. 2004 Oct 27;24(43):9541-52
references.pmid: 15509741
references.citation: J Neurosci. 1997 Nov 1;17(21):8363-75
references.pmid: 9334410
references.citation: Cereb Cortex. 2003 Oct;13(10):1110-21
references.pmid: 12967927
references.citation: Exp Neurol. 2004 Oct;189(2):222-30
references.pmid: 15380474
references.citation: Eur J Neurosci. 2002 Sep;16(5):836-42
references.pmid: 12372019
references.citation: Brain Res Mol Brain Res. 2000 Sep 30;81(1-2):19-28
references.pmid: 11000475
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):12161-6
references.pmid: 16880397
references.citation: J Biol Chem. 2005 Oct 14;280(41):34924-32
references.pmid: 16079146
references.citation: Neuroreport. 2001 May 8;12(6):1175-9
references.pmid: 11338187