HOXD13 binds DNA replication origins to promote origin licensing and is inhibited by geminin.
HOX DNA-binding proteins control patterning during development by regulating processes such as cell aggregation and proliferation. Recently, a possible involvement of HOX proteins in replication origin activity was suggested by results showing that a number of HOX proteins interact with the DNA replication licensing regulator geminin and bind a characterized human origin of replication. The functional significance of these observations, however, remained unclear. We show that HOXD13, HOXD11, and HOXA13 bind in vivo all characterized human replication origins tested. We furthermore show that HOXD13 interacts with the CDC6 loading factor, promotes pre-replication complex (pre-RC) proteins assembly at origins, and stimulates DNA synthesis in an in vivo replication assay. HOXD13 expression in cultured cells accelerates DNA synthesis initiation in correlation with the earlier pre-RC recruitment onto origins during G(1) phase. Geminin, which interacts with HOXD13 as well, blocks HOXD13-mediated assembly of pre-RC proteins and inhibits HOXD13-induced DNA replication. Our results uncover a function for Hox proteins in the regulation of replication origin activity and reveal an unforeseen role for the inhibition of HOX protein activity by geminin in the context of replication origin licensing.
Salsi
Valentina
V
Department of Animal Biology, University of Modena and Reggio Emilia, Via G. Campi 213/d, Modena 41100, Italy.
Ferrari
Silvia
S
Ferraresi
Roberta
R
Cossarizza
Andrea
A
Grande
Alexis
A
Zappavigna
Vincenzo
V
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D016193:G1 Phase; D064248:Geminin; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D018741:Replication Origin / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
C103706:CDC6 protein, human; D018797:Cell Cycle Proteins; C577995:GMNN protein, human; D064248:Geminin; C513184:HOXD13 protein, human; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D004247:DNA
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