IL-10 is a negative regulatory factor of CAWS-vasculitis in CBA/J mice as assessed by comparison with Bruton's tyrosine kinase-deficient CBA/N mice.
Candida albicans water-soluble fraction (CAWS), a mannoprotein-beta-glucan complex obtained from the culture supernatant of C. albicans NBRC1385, exhibits vasculitis-inducing activity (CAWS-vasculitis) in mice. The sensitivity to CAWS-vasculitis varies greatly among mouse strains. This study examined the factors contributing to or inhibiting CAWS-vasculitis using CAWS-vasculitis-resistant CBA/J mice and Bruton's tyrosine kinase-deficient CBA/N mice, which is a CAWS-vasculitis-sensitive strain that has the same origin as CBA/J mice. After stimulation with various kinds of pathogen-associated molecular patterns, the production of inflammatory cytokines IL-6 and IFN-gamma was induced in CBA/N mice, whereas that of immunosuppressive IL-10 was induced in CAWS-vasculitis-resistant CBA/J mice. Furthermore, the production of tissue inhibitor of metalloproteinase 1, an endogenous matrix metalloproteinase inhibitor, was observed in CBA/J mice. The results strongly suggest that the difference in the production of these cytokines is closely linked to the development of CAWS-vasculitis.
Miura
Noriko N
NN
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan.
Komai
Motohiko
M
Adachi
Yoshiyuki
Y
Osada
Naoki
N
Kameoka
Yosuke
Y
Suzuki
Kazuo
K
Ohno
Naohito
N
D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D002176:Candida albicans / Q000737:chemistry / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D020869:Gene Expression Profiling; D007113:Immunity, Innate / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000502:physiology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D020411:Oligonucleotide Array Sequence Analysis; D011505:Protein-Tyrosine Kinases / Q000172:deficiency* / Q000235:genetics; D013045:Species Specificity; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis; D014657:Vasculitis / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology / Q000517:prevention & control*
D016207:Cytokines; D019715:Tissue Inhibitor of Metalloproteinase-1; D016753:Interleukin-10; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625951:Btk protein, mouse