title: The effects of infrared low-level laser therapy on healing of partial osteotomy of tibia in streptozotocin-induced diabetic rats.
abstract: OBJECTIVE
  The effects of low-level laser therapy (LLLT) on a bone defect model in streptozotocin-induced diabetic (STZ-D) rats was examined.
  
  
  BACKGROUND
  LLLT accelerates bone fracture repair in healthy animals, but its effect in diabetic animals is unclear.
  
  
  METHODS
  Twenty-eight rats were divided into five groups: 1 (diabetes, no LLLT), 2 (diabetes, LLLT high dose), 3 (diabetes, LLLT low dose), 4 (no diabetes, no LLLT), and 5 (no diabetes, LLLT low dose) Diabetes was induced by a single injection of STZ in rats of groups 1, 2, and 3. A bone defect was made in the right tibia of rats in all groups. The defect in groups 2, 3, and 5 was treated with LLLT (890 nm, 70 W, 3000 Hz, circular beam shape, and 1 cm(2) spot size). Doses of 23.3 J/cm(2) (530 s) for group 2 and 11.6 J/cm(2) (265 s) for groups 3 and 5 were applied three times a week. The right tibias were collected 42 days after surgery and subjected to three-point bending test on a material testing machine (MTM) until fracture occurred. Data was automatically recorded on the MTM formed the load-deformation curve.
  
  
  RESULTS
  Mann-Whitney test showed that LLLT with 11.6 J/cm(2) significantly increased bending stiffness and maximum force in diabetic rats compared with group 1 (both p = 0.041).
  
  
  CONCLUSIONS
  LLLT in an experimental diabetic model enhanced bone repair with a higher bending stiffness and maximum force compared to the control group.
authors.affiliation: Anatomy Department, Medical Faculty, Shahid Beheshti University, MC, Tehran, Iran.
authors.forename: Farshad
authors.initials: F
authors.lastname: Javadieh
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
authors.forename: Shabnam
authors.initials: S
authors.lastname: Abdi
authors.forename: Zhaleh
authors.initials: Z
authors.lastname: Mohsenifar
authors.forename: Shila
authors.initials: S
authors.lastname: Razi
mesh_terms: D000818:Animals; D003920:Diabetes Mellitus / Q000139:chemically induced; D004195:Disease Models, Animal; D017102:Fracture Healing / Q000528:radiation effects*; D050723:Fractures, Bone / Q000532:radiotherapy*; D007259:Infrared Rays / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D008297:Male; D010027:Osteotomy; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin / Q000009:adverse effects; D013977:Tibia / Q000293:injuries* / Q000601:surgery; D014118:Toxins, Biological / Q000009:adverse effects
chemical_list: D014118:Toxins, Biological; D013311:Streptozocin