title: Determination of glycosylation sites and site-specific heterogeneity in glycoproteins.
abstract: Glycosylation is one of the most common post-translational modifications (PTMs) of proteins. At least 50% of human proteins are glycosylated with some estimates being as high as 70%. Glycoprotein analysis requires determining both the sites of glycosylation as well as the glycan structures associated with each site. Recent advances have led to the development of new analytical methods that employ mass spectrometry extensively making it possible to obtain the glycosylation site and the site microheterogeneity. These tools will be important for the eventual development of glycoproteomics.
authors.affiliation: Department of Chemistry, University of California, Davis, CA 95616, USA.
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: An
authors.forename: John W
authors.initials: JW
authors.lastname: Froehlich
authors.forename: Carlito B
authors.initials: CB
authors.lastname: Lebrilla
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000032:analysis*; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D011134:Polysaccharides / Q000032:analysis*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides
references.citation: Anal Biochem. 2004 Sep 1;332(1):178-86
references.pmid: 15301963
references.citation: Nat Biotechnol. 2003 Jun;21(6):660-6
references.pmid: 12754519
references.citation: Anal Chem. 2003 Jul 1;75(13):3256-62
references.pmid: 12964777
references.citation: Mass Spectrom Rev. 2005 Mar-Apr;24(2):232-64
references.pmid: 15389860
references.citation: J Proteome Res. 2004 May-Jun;3(3):556-66
references.pmid: 15253437
references.citation: J Proteome Res. 2006 Jul;5(7):1792-802
references.pmid: 16823988
references.citation: Mass Spectrom Rev. 2009 Mar-Apr;28(2):192-206
references.pmid: 18979527
references.citation: Anal Chem. 2007 Aug 15;79(16):6064-73
references.pmid: 17630715
references.citation: J Sep Sci. 2008 May;31(9):1585-93
references.pmid: 18435510
references.citation: Proteomics. 2001 Feb;1(2):340-9
references.pmid: 11680880
references.citation: Anal Chem. 2008 May 15;80(10):3684-92
references.pmid: 18363335
references.citation: Expert Rev Proteomics. 2005 Jan;2(1):87-101
references.pmid: 15966855
references.citation: Anal Chem. 1997 Oct 1;69(19):3986-94
references.pmid: 9322435
references.citation: J Proteome Res. 2006 Oct;5(10):2800-8
references.pmid: 17022651
references.citation: Methods Mol Biol. 2006;328:177-85
references.pmid: 16785649
references.citation: Bioessays. 1999 May;21(5):412-21
references.pmid: 10376012
references.citation: J Biomol Tech. 2005 Dec;16(4):407-13
references.pmid: 16522863
references.citation: Anal Chem. 2001 Sep 15;73(18):4530-6
references.pmid: 11575803
references.citation: Rapid Commun Mass Spectrom. 2007;21(6):1053-61
references.pmid: 17311219
references.citation: Anal Chem. 1993 Apr 1;65(7):877-84
references.pmid: 8470819
references.citation: J Proteome Res. 2007 Oct;6(10):3995-4005
references.pmid: 17727280
references.citation: Anal Biochem. 1996 Feb 1;234(1):38-49
references.pmid: 8742080
references.citation: Rapid Commun Mass Spectrom. 2005;19(21):3002-6
references.pmid: 16196021
references.citation: Curr Opin Struct Biol. 2005 Oct;15(5):517-24
references.pmid: 16143513
references.citation: Mass Spectrom Rev. 2009 Mar-Apr;28(2):273-361
references.pmid: 18825656
references.citation: J Proteome Res. 2007 Oct;6(10):4032-40
references.pmid: 17824634
references.citation: J Proteome Res. 2009 Feb;8(2):502-12
references.pmid: 19072223
references.citation: Rapid Commun Mass Spectrom. 2008 Oct;22(20):3245-54
references.pmid: 18803335
references.citation: J Proteome Res. 2006 Mar;5(3):701-8
references.pmid: 16512686
references.citation: J Am Soc Mass Spectrom. 2002 Sep;13(9):1065-77
references.pmid: 12322954
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):115-28
references.pmid: 17049937
references.citation: Biochem Biophys Res Commun. 2006 Nov 24;350(3):580-7
references.pmid: 17022936
references.citation: Glycobiology. 2009 Jul;19(7):707-14
references.pmid: 19270074
references.citation: Proteomics. 2004 Feb;4(2):454-65
references.pmid: 14760718
references.citation: J Proteomics. 2008 Aug 21;71(3):304-17
references.pmid: 18638581
references.citation: J Proteome Res. 2009 Feb;8(2):493-501
references.pmid: 19067536
references.citation: Anal Chem. 2003 Oct 15;75(20):5628-37
references.pmid: 14710847
references.citation: Mol Cell Proteomics. 2005 Dec;4(12):1977-89
references.pmid: 16170054
references.citation: Rapid Commun Mass Spectrom. 2007;21(5):691-8
references.pmid: 17279605
references.citation: Glycobiology. 2008 Apr;18(4):339-49
references.pmid: 18272656
references.citation: J Am Soc Mass Spectrom. 2003 Jul;14(7):777-84
references.pmid: 12837600
references.citation: Biochim Biophys Acta. 2006 Dec;1764(12):1853-69
references.pmid: 17134948
references.citation: Biochim Biophys Acta. 2005 May 25;1723(1-3):229-39
references.pmid: 15814299
references.citation: Analyst. 2008 Jun;133(6):731-8
references.pmid: 18493671
references.citation: Rapid Commun Mass Spectrom. 2006;20(23):3557-65
references.pmid: 17091533