title: Generation of stable co-cultures of vascular cells in a honeycomb alginate scaffold.
abstract: Scaffold-guided vascular tissue engineering has been investigated as a means to generate functional and transplantable vascular tissue grafts that increase the efficacy of cell-based therapeutic strategies in regenerative medicine. In this study, we employed confocal microscopy and three-dimensional reconstruction to assess the engraftment and growth potential of vascular cells within an alginate scaffold with aligned pores. We fabricated honeycomb alginate scaffolds with aligned pores, whose surface was immobilized with fibronectin and subsequently coated with matrigel. Endothelial cells were seeded into aligned pore scaffolds in the presence and absence of human smooth muscle cells. We showed that endothelial cells seeded into alginate scaffolds attach on the surface of aligned pores in vitro, giving rise to stable co-cultures of vascular cells. Moreover, the three-dimensional alginate depots containing the cells were exposed to laminar flow in order to recapitulate physiological shear stress found in the vasculature in vivo. After the flow exposure, the scaffold remained intact and some cells remained adherent to the scaffold and aligned in the flow direction. These studies demonstrate that alginate scaffolds provide a suitable matrix for establishing durable angiogenic modules that may ultimately enhance organ revascularization.
authors.affiliation: Department of Genetics Medicine, Weill Medical College of Cornell University, New York, New York, USA.
authors.forename: Masaya
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Daylon
authors.initials: D
authors.lastname: James
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jason
authors.initials: J
authors.lastname: Butler
authors.forename: Shahin
authors.initials: S
authors.lastname: Rafii
authors.forename: Sina
authors.initials: S
authors.lastname: Rabbany
mesh_terms: D000464:Alginates; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D018919:Neovascularization, Physiologic; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
references.citation: Biomacromolecules. 2004 Sep-Oct;5(5):1720-7
references.pmid: 15360280
references.citation: Biotechnol Bioeng. 1998 Jan 5;57(1):46-54
references.pmid: 10099177
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19288-93
references.pmid: 19036927
references.citation: Angew Chem Int Ed Engl. 2007;46(25):4689-92
references.pmid: 17503408
references.citation: Pharm Res. 2003 Aug;20(8):1103-12
references.pmid: 12948005
references.citation: J Biomed Mater Res. 2001 Sep 5;56(3):368-75
references.pmid: 11372054
references.citation: Br J Surg. 2005 Jun;92(6):665-72
references.pmid: 15912490
references.citation: J Mater Sci Mater Med. 1999 Dec;10(12):773-7
references.pmid: 15347949
references.citation: J Biomed Mater Res. 2000 Sep 5;51(3):376-82
references.pmid: 10880079
references.citation: Nat Rev Drug Discov. 2002 Mar;1(3):177-86
references.pmid: 12120502
references.citation: Curr Top Dev Biol. 2004;64:181-205
references.pmid: 15563948
references.citation: Biomaterials. 2001 Dec;22(23):3169-78
references.pmid: 11603589
references.citation: J Biomed Mater Res. 2000 Dec 5;52(3):576
references.pmid: 11007628
references.citation: Tissue Eng. 2000 Feb;6(1):39-44
references.pmid: 10941199
references.citation: Nat Biotechnol. 2005 Jan;23(1):47-55
references.pmid: 15637621
references.citation: Biotechnol Bioeng. 2008 Apr 1;99(5):1230-40
references.pmid: 17929323
references.citation: Curr Opin Biotechnol. 2004 Oct;15(5):381-2
references.pmid: 15464365
references.citation: J Biomed Mater Res. 2000 Aug;51(2):233-40
references.pmid: 10825223
references.citation: J Vasc Surg. 2005 Feb;41(2):349-54
references.pmid: 15768021
references.citation: Eur J Vasc Endovasc Surg. 1996 Jul;12(1):46-53
references.pmid: 8696896
references.citation: Biomaterials. 1998 Aug;19(15):1405-12
references.pmid: 9758040
references.citation: Science. 1993 May 14;260(5110):920-6
references.pmid: 8493529
references.citation: Nature. 2004 Apr 1;428(6982):487-92
references.pmid: 15057821
references.citation: Biosens Bioelectron. 2007 Jun 15;22(12):2803-11
references.pmid: 17240132
references.citation: Clin Plast Surg. 2003 Oct;30(4):573-9
references.pmid: 14621305
references.citation: Am J Physiol Heart Circ Physiol. 2005 Apr;288(4):H1915-24
references.pmid: 15576436
references.citation: Artif Cells Blood Substit Immobil Biotechnol. 1996 Jan;24(1):51-63
references.pmid: 8714719
references.citation: Clin Plast Surg. 2003 Oct;30(4):589-99
references.pmid: 14621307
references.citation: J Biomed Mater Res A. 2003 Nov 1;67(2):647-57
references.pmid: 14566809
references.citation: Biorheology. 1984;21(4):617-30
references.pmid: 6487771
references.citation: J Biol Chem. 2004 Mar 19;279(12):11760-6
references.pmid: 14660586
references.citation: Anal Biochem. 1992 Nov 15;207(1):186-92
references.pmid: 1489093
references.citation: Tissue Eng. 2007 Aug;13(8):1837-44
references.pmid: 17590149
references.citation: J Vasc Surg. 2007 Jun;45 Suppl A:A104-15
references.pmid: 17544031