Proteolysis activation and proteome alterations in murine skeletal muscle submitted to 1 week of hindlimb suspension.
The aim of this study was to investigate the temporal involvement of different proteolytic systems and muscle proteome changes during experimental disuse atrophy (up to 1 week hindlimb suspension, HS) in murine gastrocnemius muscle. The results showed that proteolysis, cytoprotection mechanisms and signs of cellular infiltration occurred very early. After 1 day of HS, signals of lysosomal activation, rather than programmed cell death (apoptosis), seem to trigger protein breakdown in the whole skeletal muscle. Moreover, the ubiquitin-proteasome pathway remained elevated later whereas all other proteolytic parameters returned to control values when atrophy was fully established. Using proteomics, evidence is provided for metabolic alterations toward glycolysis and for cytoskeleton remodelling suggestive of reduced capacity for force generation. Overall, our data highlight an early and coordinated time-dependent activation of proteolysis, which explains the global proteome alterations observed in gastrocnemius under atrophic conditions.
Ferreira
Rita
R
CIAFEL, Faculty of Sports Sciences, University of Porto, Porto, Portugal. ritaferreira@ua.pt
Vitorino
Rui
R
Neuparth
Maria João
MJ
Appell
Hans-Joachim
HJ
Duarte
José Alberto
JA
Amado
Francisco
F
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D019417:Hindlimb Suspension / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D009133:Muscular Atrophy / Q000378:metabolism / Q000473:pathology; D009929:Organ Size; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics
D015415:Biomarkers; D020543:Proteome
Physiol Rev. 2003 Jul;83(3):731-801
12843408
Proteomics. 2008 Sep;8(17):3588-604
18686300
Eur J Biochem. 2003 Feb;270(4):589-99
12581199
Arch Biochem Biophys. 2004 May 1;425(1):42-50
15081892
Exp Physiol. 2007 May;92(3):561-73
17272355
Am J Physiol Cell Physiol. 2004 Oct;287(4):C834-43
15355854
Physiol Rev. 2002 Apr;82(2):373-428
11917093
Am J Physiol Regul Integr Comp Physiol. 2005 Feb;288(2):R337-44
15637170
Physiol Rev. 2000 Jul;80(3):1215-65
10893434
J Clin Invest. 2004 Jan;113(1):115-23
14702115
J Nutr. 1999 Jan;129(1S Suppl):227S-237S
9915905
Pflugers Arch. 1998 May;435(6):804-9
9518509
Electrophoresis. 2000 Nov;21(17):3666-72
11271485
Am J Physiol Cell Physiol. 2007 May;292(5):C1723-31
17182728
Muscle Nerve. 2006 Feb;33(2):155-65
16228971
Am J Physiol Regul Integr Comp Physiol. 2005 Jul;289(1):R4-14
15956763
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Apr 5;769(2):323-32
11996498
J Physiol. 2005 May 15;565(Pt 1):309-23
15774533
FASEB J. 2001 Mar;15(3):684-92
11259386
Electrophoresis. 1998 May;19(5):758-60
9629911
J Biol Chem. 1990 May 25;265(15):8550-7
2187867
Eur J Appl Physiol. 2007 Jul;100(4):445-55
17429681
FASEB J. 2005 Jul;19(9):1143-5
15831715
Cell. 2006 Apr 7;125(1):187-99
16615899
Int J Biochem Cell Biol. 2005 Oct;37(10):2098-114
16125113
Anal Biochem. 2007 Jul 15;366(2):156-69
17540331
Am J Physiol Endocrinol Metab. 2005 Dec;289(6):E969-80
16046454
Int J Biochem Cell Biol. 2005 Oct;37(10):1974-84
16087388
J Neurosci. 1999 Sep 15;19(18):7860-9
10479688
J Biol Chem. 1996 Oct 25;271(43):26690-7
8900146
Am J Physiol Regul Integr Comp Physiol. 2005 Jun;288(6):R1423-31
15886351
J Appl Physiol (1985). 1995 Nov;79(5):1796-802
8594043
Am J Physiol Cell Physiol. 2000 Sep;279(3):C639-47
10942714
Pflugers Arch. 2007 Jul;454(4):625-33
17334780
J Appl Physiol (1985). 1999 Jul;87(1):386-90
10409599
Science. 2001 Nov 23;294(5547):1704-8
11679633
J Appl Physiol (1985). 2002 Apr;92(4):1367-77
11895999
Biochem J. 2006 Jul 1;397(1):149-58
16483253
Mol Cell Biol. 2003 Dec;23(24):9127-35
14645524
J Appl Physiol Respir Environ Exerc Physiol. 1979 Nov;47(5):974-7
511723
Int J Biochem Cell Biol. 2005 Oct;37(10):1962-73
15905114
Biochem J. 1996 May 15;316 ( Pt 1):65-72
8645234
Eur J Appl Physiol. 2007 Oct;101(3):331-40
17624543
Am J Phys Med Rehabil. 2002 Nov;81(11 Suppl):S127-47
12409818
Am J Physiol Cell Physiol. 2001 Oct;281(4):C1285-90
11546666