title: The expression of tumstatin is down-regulated in renal carcinoma.
abstract: Tumstatin is the 28 kDa NC1 domain of the alpha3 chain of type IV collagen that inhibits pathological angiogenesis and suppresses endothelial cell proliferation and tumor growth. In the present paper, we expressed and purified recombinant human tumstatin protein and then prepared the anti-tumstatin polyclonal antibody. To investigate the expression of tumstatin in renal carcinoma, tumstatin protein was detected by western blotting using the prepared anti-tumstatin antibody and tumstatin mRNA levels were assayed by RT-PCR. The results showed that the expression of tumstatin gene was down-regulated in renal carcinoma tissues and cells. Our study suggests that as a novel endogenous angiogenesis inhibitor, tumstatin gene expression may be a marker for diagnosis, therapy and prognosis of renal carcinoma.
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, 44#, Wenhua Xi Road, 250012 Jinan, Shandong, China.
authors.forename: Chun-xiao
authors.initials: CX
authors.lastname: Xu
authors.forename: Xian-xi
authors.initials: XX
authors.lastname: Liu
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Hou
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Yan
authors.forename: Shi-min
authors.initials: SM
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Guang-shui
authors.initials: GS
authors.lastname: Jiang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Jia-xuan
authors.initials: JX
authors.lastname: Xin
mesh_terms: D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D001324:Autoantigens / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D024141:Collagen Type IV / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007680:Kidney Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology
chemical_list: D000912:Antibodies, Neoplasm; D001324:Autoantigens; D024141:Collagen Type IV; C057112:type IV collagen alpha3 chain
references.citation: Cell. 1997 Jan 24;88(2):277-85
references.pmid: 9008168
references.citation: J Urol. 2000 Jan;163(1):343-7
references.pmid: 10604387
references.citation: Pattern Recognit. 2009 Jun 1;42(6):1149-1161
references.pmid: 19492069
references.citation: Cell. 1996 Aug 9;86(3):353-64
references.pmid: 8756718
references.citation: Protein Expr Purif. 2006 Jun;47(2):461-6
references.pmid: 16503164
references.citation: J Biol Chem. 2001 Aug 24;276(34):31959-68
references.pmid: 11399763
references.citation: Cancer Cell. 2003 Jun;3(6):589-601
references.pmid: 12842087
references.citation: Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4766-71
references.pmid: 12682293
references.citation: J Biol Chem. 2001 May 4;276(18):15240-8
references.pmid: 11278365
references.citation: Nat Med. 1995 Jan;1(1):27-31
references.pmid: 7584949
references.citation: Nature. 2005 Dec 15;438(7070):932-6
references.pmid: 16355210
references.citation: N Engl J Med. 1971 Nov 18;285(21):1182-6
references.pmid: 4938153
references.citation: J Biol Chem. 2000 Aug 4;275(31):23745-50
references.pmid: 10837460
references.citation: Nature. 2000 Sep 14;407(6801):249-57
references.pmid: 11001068
references.citation: J Biol Chem. 1992 Jun 5;267(16):10931-4
references.pmid: 1375931
references.citation: J Biol Chem. 1993 Dec 15;268(35):26033-6
references.pmid: 8253711
references.citation: Exp Cell Res. 2004 Dec 10;301(2):251-65
references.pmid: 15530861
references.citation: J Natl Cancer Inst. 1995 Nov 1;87(21):1603-12
references.pmid: 7563203
references.citation: Oral Oncol. 2008 Dec;44(12):1118-26
references.pmid: 18485794
references.citation: Eur J Surg Oncol. 2003 Dec;29(10):879-83
references.pmid: 14624781
references.citation: J Biochem Mol Biol. 2003 Jan 31;36(1):49-59
references.pmid: 12542975
references.citation: Differentiation. 1998 Jul;63(3):125-30
references.pmid: 9697306
references.citation: Biochem Biophys Res Commun. 2005 Jul 29;333(2):292-8
references.pmid: 15979458
references.citation: Oncology. 2005;69 Suppl 3:4-10
references.pmid: 16301830
references.citation: Science. 2002 Jan 4;295(5552):140-3
references.pmid: 11778052
references.citation: J Biol Chem. 1997 Jul 4;272(27):17023-32
references.pmid: 9202017
references.citation: J Biol Chem. 2000 Jul 14;275(28):21340-8
references.pmid: 10766752