An RNA-dependent RNA polymerase formed by TERT and the RMRP RNA.
Constitutive expression of telomerase in human cells prevents the onset of senescence and crisis by maintaining telomere homeostasis. However, accumulating evidence suggests that the human telomerase reverse transcriptase catalytic subunit (TERT) contributes to cell physiology independently of its ability to elongate telomeres. Here we show that TERT interacts with the RNA component of mitochondrial RNA processing endoribonuclease (RMRP), a gene that is mutated in the inherited pleiotropic syndrome cartilage-hair hypoplasia. Human TERT and RMRP form a distinct ribonucleoprotein complex that has RNA-dependent RNA polymerase (RdRP) activity and produces double-stranded RNAs that can be processed into small interfering RNA in a Dicer (also known as DICER1)-dependent manner. These observations identify a mammalian RdRP composed of TERT in complex with RMRP.
Maida
Yoshiko
Y
Cancer Stem Cell Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
Yasukawa
Mami
M
Furuuchi
Miho
M
Lassmann
Timo
T
Possemato
Richard
R
Okamoto
Naoko
N
Kasim
Vivi
V
Hayashizaki
Yoshihide
Y
Hahn
William C
WC
Masutomi
Kenkichi
K
D002460:Cell Line; D004722:Endoribonucleases / Q000235:genetics*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D012330:RNA, Double-Stranded / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry* / Q000378:metabolism*; D043244:Ribonuclease III / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
C544035:RMRP non-coding RNA, human; D012330:RNA, Double-Stranded; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D022661:RNA, Untranslated; D012261:Ribonucleoproteins; D012324:RNA-Dependent RNA Polymerase; C509186:TERT protein, human; D019098:Telomerase; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease; D043244:Ribonuclease III
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