Conditioned medium from neonatal rat olfactory ensheathing cells promotes the survival and proliferation of spiral ganglion cells.
CONCLUSIONS
Neonatal olfactory ensheathing cells conditioned medium (OEC-CM) can promote growth and proliferation of neonatal spiral ganglion cells (SGCs), as well as protecting them from apoptosis.


OBJECTIVE
To investigate the effects of neonatal OEC-CM on the survival, apoptosis, and proliferation of spiral ganglion.


METHODS
SGCs were cultured with OEC-CM, the conditioned medium from olfactory nerve fibroblasts (ONF-CM) or control medium containing 10% serum (DF12-10S). After different periods of culture, the cell types present were identified by immunofluorescent confocal imaging analysis, cell proliferation was determined by BrdU incorporation, and apoptosis was detected with the double-labeled NeuroTACS in situ Apoptosis Detection Kit.


RESULTS
SGCs cultured with regular control medium died off via apoptosis. Culturing cells with ONF-CM had no significant effects on the natural process of survival and apoptosis of SGCs. However, culture with OEC-CM significantly increased survival and decreased the apoptotic percentage of SGCs. In addition, OEC-CM temporarily stimulated the proliferative capacity of SGCs on the third day of culture.
Yu
Hongmeng
H
Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai.
Ye
Jing
J
Li
Huawei
H
Zhang
Jian
J
Jiang
Hongqun
H
Dai
Chunfu
C
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology; D002452:Cell Count; D002455:Cell Division / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured / Q000166:cytology; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000166:cytology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins; D009832:Olfactory Nerve / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000032:analysis; D013136:Spiral Ganglion / Q000166:cytology*
D017077:Culture Media, Conditioned; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; C068313:Ngfr protein, rat