Developmental change of Na(+)-absorptive function in Reissner's membrane epithelia.
The purpose of this study was to investigate the developmental change of epithelial Na channel function in epithelial cells of rat Reissner's membrane. The Reissner's membrane was isolated from the upper turn of the cochlea at different stages of development. The vibrating probe technique was chosen to measure transepithelial currents under short circuit conditions. Amiloride-sensitive Na current in the Reissner's membrane developed postnatally between days 3 and 5, which was completely blocked by amiloride. This tendency remained from days 5 through 14. Development of Na transport in epithelial cells of the Reissner's membrane may contribute considerably to the accomplishment of low Na concentration in the endolymph during early neonatal period.
Kim
Chang-Hee
CH
Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul National University Hospital and Sensory Organ Research Institute, Seoul National University Medical Research Center, Seoul 110-744, Korea.
Kim
Hye-Young
HY
O Chang
Sun
S
Oh
Seung-Ha
SH
Lee
Ji-Eun
JE
Lee
Jun Ho
JH
D000375:Aging; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D003051:Cochlea / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D004710:Endolymph / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D062686:Epithelial Sodium Channel Blockers; D053503:Epithelial Sodium Channels / Q000378:metabolism; D066298:In Vitro Techniques; D008839:Microelectrodes; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D026941:Sodium Channel Blockers / Q000494:pharmacology; D014732:Vibration
D062686:Epithelial Sodium Channel Blockers; D053503:Epithelial Sodium Channels; D026941:Sodium Channel Blockers; D000584:Amiloride; D012964:Sodium