title: Application of iTRAQ to catalogue the skeletal muscle proteome in pigs and assessment of effects of gender and diet dephytinization.
abstract: In this study iTRAQ was used to produce a highly confident catalogue of 542 proteins identified in porcine muscle (false positive<5%). To our knowledge this is the largest reported set of skeletal muscle proteins in livestock. Comparison with human muscle proteome demonstrated a low level of false positives with 83% of the proteins common to both proteomes. In addition, for the first time we assess variations in the muscle proteome caused by sexually dimorphic gene expression and diet dephytinization. Preliminary analysis identified 19 skeletal muscle proteins differentially expressed between male and female pigs (> or = 1.2-fold, p<0.05), but only one of them, GDP-dissociation inhibitor 1, was significant (p<0.05) after false discovery rate correction. Diet dephytinization affected expression of 20 proteins (p<0.05). This study would contribute to an evaluation of the suitability of the pig as a model to study human gender-related differences in gene expression. Transgenic pigs used in this study might also serve as a useful model to understand changes in human physiology resulting from diet dephytinization.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, ON, Canada.
authors.forename: Hatam A
authors.initials: HA
authors.lastname: Hakimov
authors.forename: Sandra
authors.initials: S
authors.lastname: Walters
authors.forename: Tom C
authors.initials: TC
authors.lastname: Wright
authors.forename: Roy G
authors.initials: RG
authors.lastname: Meidinger
authors.forename: Chris P
authors.initials: CP
authors.lastname: Verschoor
authors.forename: Moshe
authors.initials: M
authors.lastname: Gadish
authors.forename: David K Y
authors.initials: DK
authors.lastname: Chiu
authors.forename: Martina V
authors.initials: MV
authors.lastname: Strömvik
authors.forename: Cecil W
authors.initials: CW
authors.lastname: Forsberg
authors.forename: Serguei P
authors.initials: SP
authors.lastname: Golovan
mesh_terms: D010832:6-Phytase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D004032:Diet; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D018482:Muscle, Skeletal / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D012737:Sex Factors; D013552:Swine
chemical_list: D020543:Proteome; D010833:Phytic Acid; D010832:6-Phytase