Preparation of conophylline affinity nano-beads and identification of a target protein.
Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing beta-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of beta-cells, and this probe should be useful to find other target proteins.
Suzuki
Eriko
E
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-0061, Japan.
Ogura
Hideki
H
Kato
Kuniki
K
Takei
Izumi
I
Kabe
Yasuaki
Y
Handa
Hiroshi
H
Umezawa
Kazuo
K
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D050417:Insulin-Secreting Cells / Q000166:cytology; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D014748:Vinca Alkaloids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology
D048868:Adaptor Proteins, Signal Transducing; C545031:Arl6ip1 protein, rat; D007004:Hypoglycemic Agents; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014748:Vinca Alkaloids; C099167:conophylline