Chitooligosaccharides protect human embryonic hepatocytes against oxidative stress induced by hydrogen peroxide.
Chitooligosaccharides (COS) has many biological activities, such as antitumor activity and hepatoprotective effect. Herein, we investigated the protective effect of COS against hydrogen peroxide (H2O2)-induced oxidative stress on human embryonic hepatocytes (L02 cells) and its scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical in vitro. The results showed that the lost cell viability induced by H2O2 was markedly restored after 24 h pre-incubation with COS (0.1-0.4 mg/ml). This rescue effect could be related to the antioxidant property of COS, in which we showed that the radical scavenging activity of COS reached 80% at concentration of 2 mg/ml. In addition, COS could prevent cell apoptosis induced by H2O2, as shown by the inhibition of the cleavage of poly (adenosine diphosphate-ribose) polymerase and increased expression of the anti-apoptotic protein Bcl-xL. Furthermore, we have utilized confocal laser microscopy to observe cellular uptake of COS, an important step for COS to exert its effects on target cells. Taken together, our findings suggested that COS could effectively protect L02 cells against oxidative stress, which might be useful in clinical setting during the treatment of oxidative stress-related liver damages.
Xu
Qingsong
Q
Dalian Institute of Chemical Physics, Chinese Academy Sciences, Zhongshan Street 457, Dalian 116023, China.
Ma
Pan
P
Yu
Weiting
W
Tan
Chengyu
C
Liu
Hongtao
H
Xiong
Chuannan
C
Qiao
Ying
Y
Du
Yuguang
Y
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D002460:Cell Line; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D009844:Oligosaccharides / Q000737:chemistry* / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
D000975:Antioxidants; C494811:BCL2L1 protein, human; D005456:Fluorescent Dyes; D009844:Oligosaccharides; D051020:bcl-X Protein; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases
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