title: Real-time PCR and enrichment culture for sensitive detection and enumeration of Escherichia coli.
abstract: Rapid enumeration of Escherichia coli strains by quantitative real-time PCR targeting the uidA gene was developed and confirmed for minced beef, tuna and raw oyster. Higher sensitivity (1 CFU/g of E. coli in all three food samples) was obtained by incubating for 7 h in TSB. Colony-directed E. coli specific TaqMan PCR assay could effectively distinguish colonies grown on various selective media within 1.5-h. Inspection of E. coli in food testing laboratories is important, and our rapid E. coli detection strategy will contribute to quality control in food industries.
authors.affiliation: Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, 5-7 Konan 4, Minato, Tokyo 108-8477, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Junko
authors.initials: J
authors.lastname: Shinozaki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Suda
authors.forename: Tateo
authors.initials: T
authors.lastname: Fujii
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins