title: Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments.
abstract: Thermal convection and high intensity solvent resonances can significantly hamper diffusion estimates in pulsed gradient spin-echo nuclear magnetic resonance diffusion experiments on biomolecule samples. To overcome these two problems, a new double functional NMR diffusion sequence, double echo PGSTE-WATERGATE, is presented. The new sequence provides excellent convection compensation and solvent suppression (with a suppression factor in excess of at least 10(5) in a single scan) in biomolecular NMR diffusion experiments. Due to its stimulated echo nature, the new sequence is much less susceptible to spin-spin relaxation than Hahn spin-echo based sequences. Furthermore, the new sequence is not susceptible to spin diffusion due to the application of bipolar pulsed gradients. The new sequence is also much easier to set up compared to previously developed stimulated echo based convection compensation and solvent suppression sequence. The utility of the new sequence is demonstrated on an aqueous lysozyme sample.
authors.affiliation: Nanoscale Organisation and Dynamics Group, College of Health and Science, University of Western Sydney, Penrith South DC, NSW, 1797, Australia.
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
authors.forename: William S
authors.initials: WS
authors.lastname: Price
mesh_terms: D004058:Diffusion; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D011506:Proteins; D012997:Solvents
references.citation: J Magn Reson. 2008 Sep;194(1):108-14
references.pmid: 18619877
references.citation: J Magn Reson. 1999 Aug;139(2):334-41
references.pmid: 10423370
references.citation: J Magn Reson. 2000 Oct;146(2):283-9
references.pmid: 11001844
references.citation: J Magn Reson. 2004 Oct;170(2):322-8
references.pmid: 15388096
references.citation: J Magn Reson. 2001 May;150(1):35-8
references.pmid: 11330980
references.citation: J Magn Reson B. 1994 May;104(1):1-10
references.pmid: 8025810
references.citation: J Magn Reson. 2004 Apr;167(2):328-33
references.pmid: 15040990
references.citation: J Magn Reson. 2001 Jun;150(2):126-31
references.pmid: 11384170
references.citation: J Magn Reson. 2005 Dec;177(2):203-11
references.pmid: 16140552
references.citation: J Magn Reson. 2008 Mar;191(1):159-63
references.pmid: 18086542
references.citation: J Biomol NMR. 2004 Jul;29(3):299-308
references.pmid: 15213428
references.citation: Anal Bioanal Chem. 2007 May;388(1):173-7
references.pmid: 17393152
references.citation: J Magn Reson. 2000 Jul;145(1):125-31
references.pmid: 10873503
references.citation: J Magn Reson. 2000 Feb;142(2):323-5
references.pmid: 10648149
references.citation: Anal Chem. 2001 Aug 1;73(15):3528-34
references.pmid: 11510814
references.citation: J Magn Reson. 2005 Jun;174(2):229-36
references.pmid: 15862239
references.citation: J Magn Reson. 2004 Jul;169(1):92-101
references.pmid: 15183361
references.citation: J Magn Reson. 1998 Mar;131(1):126-30
references.pmid: 9533914
references.citation: J Magn Reson. 2006 Jun;180(2):266-73
references.pmid: 16563821
references.citation: J Magn Reson. 2001 Nov;153(1):48-55
references.pmid: 11700080