Liver X receptor β: maintenance of epidermal expression in intrinsic and extrinsic skin aging.
Aging in human skin is the composite of time-dependent intrinsic aging plus photoaging induced by chronic exposure to ultraviolet radiation. Nuclear hormone receptors coordinate diverse processes including metabolic homeostasis. Liver X receptor β (LXRβ) is a close human homologue of daf-12, a regulator of nematode longevity. LXRβ is positively regulated by sirtuin-1 and resveratrol, while LXRβ-null mice show transcriptional profiles similar to those seen in aged human skin. In these studies, we examined LXRβ expression in aged and photoaged human skin. Volunteers were recruited to assess intrinsic aging and photoaging. Epidermal LXRβ mRNA was examined by in situ hybridization while protein was identified by immunofluorescence. No significant changes were observed in either LXRβ mRNA or protein expression between young and aged volunteers (mRNA p = 0.90; protein p = 0.26). Similarly, LXRβ protein expression was unaltered in photoaged skin (p = 0.75). Our data therefore suggest that, while not playing a major role in skin aging, robust cutaneous expression implies a fundamental role for LXRβ in epidermal biology.
Ford
Christopher T
CT
Dermatological Sciences, The University of Manchester, 1.443 Stopford Building, Oxford Road, Manchester, UK.
Sherratt
Michael J
MJ
Griffiths
Christopher E M
CE
Watson
Rachel E B
RE
D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D015153:Blotting, Western; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D000071518:Liver X Receptors; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D057093:Orphan Nuclear Receptors / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis; D015595:Skin Aging / Q000235:genetics*; D055815:Young Adult
D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear
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