SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods.
Antibody-conjugated gold nanorods (GNRs) have been used for the targeting and imaging of specific cancer markers expressed on the surface membrane of cancer cells. GNRs with various aspect ratios were fabricated, and their surface-enhanced Raman scattering enhancement effects were evaluated. To attach the GNRs selectively onto the targets in cancer cells, specific antibodies were immobilized on the surface of GNRs using the layer-by-layer deposition method. First, Raman reporter molecules, mercaptopyridine, were attached to the surface of GNRs, and their surface charge was modified using poly(sodium 4-styrene-sulfonate) to make the surface charge negative. Then, anti-rabbit IgGs were immobilized onto the surface of the GNRs by electrostatic interactions. HER2 markers, expressed on the cell surface, were treated with anti-HER2 primary antibodies. Finally, the functionalized nanoprobes, conjugated with secondary antibodies, were attached to the markers on cancer cells by antibody-antibody interactions. In the present study, MCF7 cells overexpressing breast cancer marker HER2 were used as the optical imaging targets. Our experimental results demonstrate the potential feasibility of antibody-conjugated GNRs for the highly sensitive targeting and imaging of biomarkers expressed on the surface membrane of cancer cells.
Park
Hyejin
H
Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
Lee
Sangyeop
S
Chen
Lingxin
L
Lee
Eun Kyu
EK
Shin
Soon Young
SY
Lee
Young Han
YH
Son
Sang Wook
SW
Oh
Chil Hwan
CH
Song
Joon Myong
JM
Kang
Seong Ho
SH
Choo
Jaebum
J
D000906:Antibodies; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D015854:Up-Regulation
D000906:Antibodies; D015415:Biomarkers; D006046:Gold; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human