Comparative cytogenetics of cichlid fishes through genomic in-situ hybridization (GISH) with emphasis on Oreochromis niloticus.
Cichlidae is the most species-rich freshwater family of Perciformes and has attracted the attention of aquarium hobbyists, aquaculturists, and sport fisherman. Oreochromis niloticus is very important in aquaculture today and is currently used in varied areas of study as an 'experimental model'. Oreochromis niloticus has been characterized using classical and molecular cytogenetic techniques, with special attention paid to heterochromatin structure and the identification of sex chromosomes. In this study, we compare the genome of O. niloticus with that of other cichlids from Africa and South America using genomic in-situ hybridization (GISH). Our results show that at least some elements comprising the pericentromeric heterochromatin of Nile tilapia are species-specific and that the sequence of the majority of the long arm of the largest chromosome pair is conserved within the tilapiine group, which is composed of the genera Tilapia, Oreochromis, and Sarotherodon. It is suggested that the extensive regions of repeated DNA in the largest chromosome pair of O. niloticus resulted from chromosome rearrangement or accumulation caused by recombination suppression during the evolutionary history of the tilapiines.
Targino Valente
Guilherme
G
Laboratório de Genômica Integrativa, Departamento de Morfologia, Instituto de Biociências, UNESP - Universidade Estadual Paulista, Botucatu, SP, Brazil.
Henrique Schneider
Carlos
C
Claudia Gross
Maria
M
Feldberg
Eliana
E
Martins
Cesar
C
D000818:Animals; D023681:Cichlids / Q000235:genetics*; D020732:Cytogenetic Analysis; D004247:DNA / Q000235:genetics*; D017403:In Situ Hybridization; D010802:Phylogeny
D004247:DNA
Proc Natl Acad Sci U S A. 1969 Oct;64(2):600-4
5261036
Science. 2003 Apr 11;300(5617):325-9
12649486
Mol Phylogenet Evol. 2005 Jan;34(1):227-44
15579395
Nature. 1994 Sep 15;371(6494):215-20
8078581
Cell. 1998 May 1;93(3):317-20
9590164
Cytogenet Genome Res. 2002;99(1-4):157-63
12900559
Anim Genet. 2007 Feb;38(1):85-6
17257199
Chromosome Res. 1999;7(4):267-75
10461872
Evolution. 1979 Mar;33(1Part1):1-14
28568056
Nature. 2007 Jun 7;447(7145):714-9
17554307
Anim Genet. 2003 Oct;34(5):379-83
14510676
Trends Genet. 1998 May;14(5):200-4
9613205
Genetica. 2002 Jun;115(2):169-77
12403171
Nature. 2004 Oct 21;431(7011):946-57
15496914
J Mol Evol. 2001 Aug;53(2):89-103
11479680
Nat Rev Genet. 2007 Jan;8(1):35-46
17173056
Chromosoma. 1999 Dec;108(7):457-68
10654084
Sex Dev. 2008;2(1):43-54
18418034
Curr Biol. 2007 Oct 9;17(19):R827-31
17925206
J Exp Zool. 2000 Apr 15;288(1):76-92
10750055
Cytogenet Genome Res. 2003;101(3-4):314-9
14685001
Chromosoma. 1997 Sep;106(4):226-32
9254724
Nat Rev Genet. 2004 Apr;5(4):288-98
15131652
Chromosoma. 2002 Mar;111(1):45-52
12068922
Micron. 2008 Jun;39(4):411-8
17395473
J Mol Evol. 1999 Jun;48(6):703-11
10229574
Curr Opin Plant Biol. 2006 Dec;9(6):647-53
17015032
Zebrafish. 2008 Summer;5(2):125-30
18554176
Z Tierpsychol. 1967 Dec;24(6):684-754
5591457