Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species.
Centromeres play an important role in chromosome transmission in eukaryotes and comprise specific DNA and proteins that form complexes called kinetochores. In tobacco, although a centromere-specific histone H3 (NtCENH3) and centromeric DNA sequence (Nt2-7) have been identified, no other kinetochore components have been determined. In this study, we isolated and characterized cDNAs encoding two centromeric proteins CENP-C and MIS12 from Nicotiana tabaccum. Two CENP-C homologues, NtCENP-C-1 and -2, isolated from N. tabaccum were similar to CENP-C from N. sylvestris and N. tomentosiformis, respectively. Similarly, two Mis12 homologues, NtMIS12-1 and -2, in N. tabaccum were shown to originate from N. sylvestris and N. tomentosiformis, respectively. Both respective homologues for CENP-C and Mis12 were expressed at the same level. This indicates that in a tetraploid species, N. tabaccum, two ancestral genes encoding the centromeric proteins participate equally in the functioning of centromeres.
Nagaki
Kiyotaka
K
Research Institute for Bioresources, Okayama University, Kurashiki, Japan. nagaki@rib.okayama-u.ac.jp
Kashihara
Kazunari
K
Murata
Minoru
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D015151:Immunoblotting; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; C492664:MIS12 protein, human; D008869:Microtubule-Associated Proteins; D010446:Peptide Fragments; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C060905:centromere protein C
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