Synthesis and biological applications of imidazolium-based polymerized ionic liquid as a gene delivery vector.
The encouraging results of preliminary toxicological studies on imidazolium-based ionic liquids provide good opportunities for the development of ionic liquids in biomedical applications. In this work, the polymerized ionic liquid poly[3-butyl-1-vinylimidazolium L-proline salt] has been synthesized as a gene vector. The interaction of poly[3-butyl-1-vinylimidazolium L-proline salt] with DNA was studied by agarose gel electrophoresis. The cell viability was determined through PI (propidium iodine) staining and flow cytometry, showing marginal toxicity toward the cells examined. The transfection efficiency was evaluated through the in vitro transfection experiment. The results indicated that the imidazolium cation had a high binding ability to DNA, and the condensed DNA in the complexes could be effectively protected against enzymatic degradation. Poly[3-butyl-1-vinylimidazolium L-proline salt] could further transfer the reporter gene into the HeLa cell and successfully mediate the gene expression without the aid of additional agent.
Zhang
Yuanyuan
Y
Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, People's Republic of China.
Chen
Xiang
X
Lan
Jingbo
J
You
Jingsong
J
Chen
Lijuan
L
D045744:Cell Line, Tumor; D003850:Deoxyribonuclease I / Q000378:metabolism; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D011392:Proline / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D014162:Transfection
D007093:Imidazoles; D052578:Ionic Liquids; D011108:Polymers; D011145:Polyvinyls; D011392:Proline; D003850:Deoxyribonuclease I