The effect of hydrolyzed gardeniae fructus extract hydrogel on the treatment of ecchymoses in a rat model.
BACKGROUND
Gardeniae fructus (GF) has been used in traditional medicine for the treatment of inflammatory disease.


OBJECTIVE
To evaluate the therapeutic effect of GF gel on the resolution of ecchymoses in rats.


METHODS
Fifty hind limbs (in 25 Sprague-Dawley rats) were evaluated. The ecchymoses were produced by dropping a 100-g weight from a height of 20 cm on the posterior side of the hind limbs 25 times. Then, 0.5 g of hydrolyzed GF extracted gel was applied to the right hind limbs and 0.5 g of gel without GF extract powder was applied to the left hind limbs. The area of the ecchymosis was measured, and histological analysis was performed.


RESULTS
The area affected by the ecchymosis after 5 days was 15% in the control group and 2% in the GF gel group (p=.002). The mean duration for the ecchymosis was 5.8 days in the control group and 4.5 days in the GF gel group. The extravasated red blood cells and inflammation were less prominent in the GF gel group than in the control group.


CONCLUSIONS
The results of this study showed that hydrolyzed gel of GF extract, containing genipin, was effective for the treatment of ecchymoses in a rat model.
Hwang
Kun
K
Department of Plastic Surgery, College of Medicine, Inha University, Incheon, Korea. jokerhg@inha.ac.kr
Choi
Hyuk Gyu
HG
Kim
Dae Joong
DJ
Hwang
Se Ho
SH
D000818:Animals; D004195:Disease Models, Animal; D004438:Ecchymosis / Q000188:drug therapy*; D032062:Gardenia; D020100:Hydrogels; D057889:Iridoid Glycosides; D039823:Iridoids / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D018514:Plant Structures; D051381:Rats; D017207:Rats, Sprague-Dawley
D020100:Hydrogels; D057889:Iridoid Glycosides; D039823:Iridoids; D010936:Plant Extracts; C007834:genipin