Bub1 and Bub3 promote the conversion from monopolar to bipolar chromosome attachment independently of shugoshin.
The eukaryotic spindle assembly checkpoint (SAC) delays anaphase in the presence of chromosome attachment errors. Bub3 has been reported to be required for SAC activity in all eukaryotes examined so far. We find that Bub3, unlike its binding partner Bub1, is not essential for the SAC in fission yeast. As Bub3 is needed for the efficient kinetochore localization of Bub1, and of Mad1, Mad2 and Mad3, this implies that most SAC proteins do not need to be enriched at the kinetochores for the SAC to function. We find that Bub3 is also dispensable for shugoshin localization to the centromeres, which is the second known function of Bub1. Instead, Bub3, together with Bub1, has a specific function in promoting the conversion from chromosome mono-orientation to bi-orientation.
Windecker
Hanna
H
Friedrich Miescher Laboratory of the Max Planck Society, Spemannstrasse 39, 72076 Tuebingen, Germany.
Langegger
Maria
M
Heinrich
Stephanie
S
Hauf
Silke
S
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism
C544653:Bub3 protein, S pombe; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029702:Schizosaccharomyces pombe Proteins; C485974:Sgo2 protein, S pombe; C495594:Bub1 protein, S pombe; D017346:Protein Serine-Threonine Kinases
Mol Cell Biol. 2002 Apr;22(8):2728-42
11909965
Genes Dev. 2000 Sep 15;14(18):2277-82
10995385
J Cell Biol. 2003 Apr 28;161(2):267-80
12719470
Genetics. 2008 Jun;179(2):785-92
18505884
Genes Dev. 2008 Sep 1;22(17):2385-99
18765790
Nat Rev Mol Cell Biol. 2002 Oct;3(10):731-41
12360190
Nat Rev Mol Cell Biol. 2006 Sep;7(9):644-56
16896351
Genetics. 1998 May;149(1):101-16
9584089
J Cell Biol. 1998 Dec 14;143(6):1603-16
9852154
Mol Biol Cell. 2008 Apr;19(4):1646-62
18256284
J Cell Biol. 2006 Jul 3;174(1):39-51
16818718
Mol Biol Cell. 2007 Jun;18(6):2216-25
17409356
Mol Biol Cell. 2008 Mar;19(3):1199-209
18094053
Mol Biol Cell. 2007 May;18(5):1657-69
17301288
J Cell Sci. 2005 Jan 1;118(Pt 1):187-98
15615783
Methods Enzymol. 1991;194:795-823
2005825
Mol Biol Cell. 2005 Jan;16(1):385-95
15525673
Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1201-6
17227844
Science. 2006 Jan 20;311(5759):388-91
16424343
J Cell Biol. 2009 Jun 1;185(5):841-58
19487456
Mol Biol Cell. 2008 Apr;19(4):1798-813
18199686
Science. 2005 Jan 7;307(5706):130-3
15637284
EMBO J. 2006 Oct 18;25(20):4888-96
17036054
Mol Biol Cell. 1999 Aug;10(8):2771-85
10436027
J Cell Sci. 2003 Feb 15;116(Pt 4):617-28
12538762
PLoS Genet. 2007 Nov;3(11):e213
18081426
Dev Cell. 2009 Jan;16(1):105-17
19154722
EMBO J. 2007 Oct 31;26(21):4475-86
17932486
J Biol Chem. 2007 Apr 13;282(15):11205-12
17289665
EMBO J. 2005 Apr 20;24(8):1621-33
15933723
Mol Biol Cell. 2009 Feb;20(4):1252-67
19109417
PLoS Biol. 2009 Jan 13;7(1):e10
19143472
Mol Cell Biol. 2004 Nov;24(22):9786-801
15509783
Cell. 1991 Aug 9;66(3):507-17
1651171
J Cell Biol. 1998 Dec 28;143(7):1775-87
9864354
Cell. 1984 Dec;39(2 Pt 1):349-58
6094012
Nat Cell Biol. 2008 Dec;10(12):1411-20
18997788
Dev Cell. 2004 Jul;7(1):45-60
15239953
J Cell Sci. 1995 Mar;108 ( Pt 3):1195-204
7622604
Genes Dev. 2007 Feb 15;21(4):420-35
17322402
Nature. 2004 Feb 5;427(6974):510-7
14730319
J Cell Sci. 2007 Oct 1;120(Pt 19):3345-51
17881496
Mol Biol Cell. 2002 Sep;13(9):3029-41
12221113
Curr Biol. 2005 Feb 8;15(3):214-25
15694304
Nat Cell Biol. 2005 Jan;7(1):93-8
15592459
Nat Rev Mol Cell Biol. 2007 May;8(5):379-93
17426725