title: Chromatographic Examinations of Tea's Protection Against Lipid Oxidative Modifications.
abstract: Ethanol metabolism is accompanied by generation of free radicals that damage cell components, especially lipids. The present study was designed to investigate the efficacy of the preventive effect of black tea on the lipid oxidative modifications in different tissues (plasma, liver, brain, kidney, stomach, lung, intestine, and spleen) of 12-month-old rats chronically intoxicated with ethanol. Ethanol intoxication caused changes in the level/activity of antioxidants that led to the significant increase in the level of lipid oxidative modification products. Oxidative modifications were estimated by measuring lipid hydroperoxides, malondialdehyde, and 4-hydroxynonenal by high-performance liquid chromatography (HPLC) and by spectrophotometric determination of conjugated dienes. These lipid-modification marker levels were increased in almost all examined tissues (3%-71%) after ethanol intoxication. Described changes were in accordance with the liver level of the most often used marker of arachidonic acid oxidation, isoprostane (8-isoPGF(2alpha)), determined by the LC/MS system. Administration of black tea to ethanol-intoxicated rats remarkably prevents the significant increase (by about 15%-42%) in concentrations of all measured parameters regarding all examined tissues, but especially the plasma, liver, brain, stomach, and spleen. The preventive effect of black tea in the other organs (kidney, lung, intestine) caused a decrease in examined markers in a smaller degree (by about 7%-28%). To determine in the liver the major constituents of black tea mainly responsible for antioxidative action such as catechins and theaflavins, which were absorbed in organism, the present study indicates their protective effect against ethanol-induced oxidative modifications of lipids.
authors.affiliation: Department of Analytical Chemistry, Medical University of Białystok, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Luczaj
authors.forename: Tomasz
authors.initials: T
authors.lastname: Welerowicz
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Skrzydlewska
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
references.citation: Free Radic Biol Med. 1999 Jun;26(11-12):1383-7
references.pmid: 10401601
references.citation: Chem Pharm Bull (Tokyo). 1986 Apr;34(4):1694-700
references.pmid: 3719870
references.citation: Toxicol Appl Pharmacol. 1987 Mar 15;87(3):403-10
references.pmid: 3564015
references.citation: J Nutr. 2003 Oct;133(10):3285S-3292S
references.pmid: 14519827
references.citation: Carcinogenesis. 2003 May;24(5):927-36
references.pmid: 12771038
references.citation: Cancer Res. 2000 Mar 15;60(6):1507-11
references.pmid: 10749113
references.citation: Adv Pharmacol. 1997;38:601-28
references.pmid: 8895826
references.citation: Br Med Bull. 1993 Jul;49(3):604-20
references.pmid: 8221026
references.citation: J Nutr. 1998 Apr;128(4):758-63
references.pmid: 9521640
references.citation: Toxicol Sci. 2003 Dec;76(2):262-70
references.pmid: 14600287
references.citation: Mutat Res. 1999 Mar 8;424(1-2):83-95
references.pmid: 10064852
references.citation: Chem Res Toxicol. 1993 Mar-Apr;6(2):134-46
references.pmid: 8477003
references.citation: J Chromatogr B Biomed Sci Appl. 2001 Sep 5;760(2):271-9
references.pmid: 11530986
references.citation: Arch Biochem Biophys. 1999 Dec 15;372(2):355-9
references.pmid: 10600175
references.citation: Exp Mol Pathol. 1984 Aug;41(1):44-57
references.pmid: 6468635
references.citation: Alcohol Alcohol. 1992 May;27(3):233-9
references.pmid: 1449558
references.citation: Free Radic Biol Med. 1996;20(7):933-56
references.pmid: 8743980
references.citation: Mutat Res. 1990 May;238(3):223-33
references.pmid: 2342513
references.citation: J Chromatogr. 1979 Mar 1;162(3):408-13
references.pmid: 528606
references.citation: J Lab Clin Med. 1967 Jul;70(1):158-69
references.pmid: 6066618
references.citation: Biofactors. 2000;13(1-4):73-9
references.pmid: 11237203
references.citation: Alcohol Alcohol. 1993 Jul;28(4):431-6
references.pmid: 8104400
references.citation: J Nutr. 2001 Jun;131(6):1731-7
references.pmid: 11385060
references.citation: Prev Med. 2005 Jun;40(6):910-8
references.pmid: 15850895
references.citation: Gastroenterology. 1990 Jan;98(1):203-10
references.pmid: 2293579
references.citation: Pathophysiology. 2000 Apr;7(1):25-32
references.pmid: 10825682
references.citation: J Nutr. 2003 Oct;133(10):3275S-84S
references.pmid: 14519826
references.citation: Br J Nutr. 1999 Apr;81(4):289-95
references.pmid: 10999016
references.citation: Prev Med. 1992 May;21(3):334-50
references.pmid: 1614995
references.citation: J Nutr Biochem. 2001 Jul;12(7):404-421
references.pmid: 11448616
references.citation: Methods Enzymol. 1984;105:331-7
references.pmid: 6727673
references.citation: J Stud Alcohol. 2006 Jul;67(4):510-8
references.pmid: 16736070
references.citation: J Agric Food Chem. 2000 Dec;48(12):5834-41
references.pmid: 11312761
references.citation: Alcohol. 2004 Jan;32(1):25-32
references.pmid: 15066700
references.citation: J Chromatogr A. 1996 Apr 5;729(1-2):207-10
references.pmid: 9004941
references.citation: Biochem J. 1994 Sep 15;302 ( Pt 3):773-9
references.pmid: 7945202
references.citation: Alcohol Clin Exp Res. 1996 Feb;20(1 Suppl):77A-85A
references.pmid: 8659698
references.citation: J Toxicol Environ Health A. 2004 Apr 9;67(7):595-606
references.pmid: 15129554
references.citation: Food Chem Toxicol. 2004 Dec;42(12):2045-51
references.pmid: 15500941
references.citation: Anal Biochem. 2000 Mar 15;279(2):164-9
references.pmid: 10706785
references.citation: Biochim Biophys Acta. 1996 Dec 13;1304(3):210-22
references.pmid: 8982267
references.citation: J Lipid Res. 2003 Oct;44(10):1940-5
references.pmid: 12867540
references.citation: Biochem Pharmacol. 1990 Aug 15;40(4):715-21
references.pmid: 2117455
references.citation: J Nutr. 2001 Sep;131(9):2248-51
references.pmid: 11533262
references.citation: Biol Pharm Bull. 1994 Jan;17(1):146-9
references.pmid: 8148805
references.citation: Free Radic Biol Med. 1991;11(1):81-128
references.pmid: 1937131
references.citation: Hepatology. 1997 Feb;25(2):351-5
references.pmid: 9021946
references.citation: J Agric Food Chem. 2000 Jul;48(7):2736-43
references.pmid: 10898615
references.citation: Alcohol Alcohol. 1994 Sep;29(5):513-22
references.pmid: 7811335
references.citation: Free Radic Biol Med. 1997;23(6):943-54
references.pmid: 9378374
references.citation: Carcinogenesis. 1996 Oct;17(10):2105-11
references.pmid: 8895475