title: Subcellular localization of overexpressed maize AChE gene in rice plant.
abstract: The ACh-mediated system consisting of acetylcholine (ACh), acetylcholine receptor (AChR) and acetylcholinesterase (AChE) is fundamental for nervous system function in animals and insects. Although plants lack a nervous system, both ACh and ACh-hydrolyzing activity have been widely recognized in the plant kingdom. The function of the plant ACh-mediated system is still unclear, despite more than 30 years of research. To understand ACh-mediated systems in plants, we previously purified maize AChE and cloned the corresponding gene from maize seedlings (Plant Physiology). In a recent paper in Planta, we also purified and cloned AChE from the legume plant siratro (Macroptilium atropurpureum). In comparison with electric eel AChE, both plant AChEs showed enzymatic properties of both animal AChE and animal butyrylcholinesterase. On the other hand, based on Pfam protein family analysis, both plant AChEs contain a consensus sequence of the lipase GDSL family, while the animal AChEs possess a distinct alpha/beta-hydrolase fold superfamily sequence, but no lipase GDSL sequence. Thus, neither plant AChE belongs to the well-known AChE family, which is distributed throughout the animal kingdom. To address the possible physiological roles of plant AChEs, we herein report our data from the immunological analysis of the overexpressed maize AChE gene in plants.
authors.affiliation: Faculty of Bioindustry; Tokyo University of Agriculture; Hokkaido, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Yoshie S
authors.initials: YS
authors.lastname: Momonoki
references.citation: Prog Neurobiol. 1993 Jul;41(1):31-91
references.pmid: 8321908
references.citation: J Neurocytol. 2003 Jun-Sep;32(5-8):743-66
references.pmid: 15034265
references.citation: Plant J. 1994 Aug;6(2):271-82
references.pmid: 7920717
references.citation: Curr Opin Cell Biol. 1995 Oct;7(5):673-80
references.pmid: 8573342
references.citation: Nat Rev Neurosci. 2001 Apr;2(4):294-302
references.pmid: 11283752
references.citation: Plant Physiol. 1997 May;114(1):47-53
references.pmid: 11536808
references.citation: Plant Physiol. 2005 Jul;138(3):1359-71
references.pmid: 15980188
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:281-309
references.pmid: 15012236
references.citation: Planta. 2008 Mar;227(4):809-22
references.pmid: 18046576
references.citation: J Membr Biol. 2002 Jul 15;188(2):107-13
references.pmid: 12172636
references.citation: Life Sci. 2007 May 30;80(24-25):2206-9
references.pmid: 17363003
references.citation: Plant Physiol. 1992 May;99(1):130-3
references.pmid: 16668839