Oxidative variables and antioxidant enzymes activities in the mdx mouse brain.
Dystrophin is a protein found at the plasmatic membrane in muscle and postsynaptic membrane of some neurons, where it plays an important role on synaptic transmission and plasticity. Its absence is associated with Duchenne's muscular dystrophy (DMD), in which cognitive impairment is found. Oxidative stress appears to be involved in the physiopathology of DMD and its cognitive dysfunction. In this regard, the present study investigated oxidative parameters (lipid and protein peroxidation) and antioxidant enzymes activities (superoxide dismutase and catalase) in prefrontal cortex, cerebellum, hippocampus, striatum and cortex tissues from male dystrophic mdx and normal C57BL10 mice. We observed (1) reduced lipid peroxidation in striatum and protein peroxidation in cerebellum and prefrontal cortex; (2) increased superoxide dismutase activity in cerebellum, prefrontal cortex, hippocampus and striatum; and (3) reduced catalase activity in striatum. It seems by our results, that the superoxide dismutase antioxidant mechanism is playing a protective role against lipid and protein peroxidation in mdx mouse brain.
Comim
Clarissa M
CM
Laboratório de Neurociências and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil.
Cassol
Omar J
OJ
Constantino
Leandra C
LC
Constantino
Larissa S
LS
Petronilho
Fabrícia
F
Tuon
Lisiane
L
Vainzof
Mariz
M
Dal-Pizzol
Felipe
F
Quevedo
João
J
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism* / Q000503:physiopathology; D001928:Brain Diseases, Metabolic / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism / Q000503:physiopathology; D003072:Cognition Disorders / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D003342:Corpus Striatum / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D016189:Dystrophin / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology; D050356:Lipid Metabolism / Q000235:genetics; D015227:Lipid Peroxidation / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D020388:Muscular Dystrophy, Duchenne / Q000150:complications / Q000378:metabolism / Q000503:physiopathology; D018384:Oxidative Stress / Q000235:genetics*; D017397:Prefrontal Cortex / Q000378:metabolism / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
D016189:Dystrophin; D002374:Catalase; D013482:Superoxide Dismutase