title: Znrg, a novel gene expressed mainly in the developing notochord of zebrafish.
abstract: The notochord, a defining characteristic of the chordate embryo is a critical midline structure required for axial skeletal formation in vertebrates, and acts as a signaling center throughout embryonic development. We utilized the digital differential display program of the National Center for Biotechnology Information, and identified a contig of expressed sequence tags (no. Dr. 83747) from the zebrafish ovary library in Genbank. Full-length cDNA of the identified gene was cloned by 5'- and 3'- RACE, and the resulting sequence was confirmed by polymerase chain reaction and sequencing. The cDNA clone contains 2,505 base pairs and encodes a novel protein of 707 amino acids that shares no significant homology with any known proteins. This gene was expressed in mature oocytes and at the one-cell stage, and persisted until the 5th day of development, as determined by RT-PCR. Transcripts were detected by whole-mount RNA in situ hybridization from the two-cell stage to 72 h of embryonic development. This gene was uniformly distributed from the cleavage stage up to the blastula stage. During early gastrulation, it was present in the dorsal region, and became restricted to the notochord and pectoral fin at 48 and 72 h of embryonic development. Based on its abundance in the notochord, we hypothesized that the novel gene may play an important role in notochord development in zebrafish; we named this gene, zebrafish notochord-related gene, or znrg.
authors.affiliation: The Laboratory of Molecular Genetics and Developmental Biology, College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Jianzhen
authors.initials: J
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Deng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005091:Exons / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D016678:Genome / Q000235:genetics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009672:Notochord / Q000196:embryology* / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D029961:Zebrafish Proteins; C552354:zgc:113210 protein, zebrafish
references.citation: BMC Dev Biol. 2008 Apr 17;8:42
references.pmid: 18419805
references.citation: Dev Biol. 2007 Jul 15;307(2):202-13
references.pmid: 17543297
references.citation: Dev Biol. 1995 Oct;171(2):655-64
references.pmid: 7556944
references.citation: Development. 2000 Feb;127(4):869-79
references.pmid: 10648245
references.citation: Development. 2002 Jul;129(14):3311-23
references.pmid: 12091302
references.citation: Dev Biol. 1997 Mar 1;183(1):37-48
references.pmid: 9119113
references.citation: Curr Top Dev Biol. 2000;47:247-77
references.pmid: 10595307
references.citation: Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14024-9
references.pmid: 14612568
references.citation: Dev Dyn. 1999 Nov;216(3):233-43
references.pmid: 10590475
references.citation: J Cell Biol. 2003 Sep 1;162(5):899-908
references.pmid: 12952940
references.citation: Mech Dev. 2001 Jul;105(1-2):157-62
references.pmid: 11429291
references.citation: Dev Biol. 2008 Apr 1;316(1):21-35
references.pmid: 18281032
references.citation: Dev Biol. 2001 Jun 1;234(1):1-12
references.pmid: 11356015
references.citation: Curr Top Dev Biol. 2005;65:229-53
references.pmid: 15642386
references.citation: J Cell Biol. 2000 Feb 21;148(4):691-702
references.pmid: 10684251
references.citation: Nature. 1995 Nov 9;378(6553):150-7
references.pmid: 7477317
references.citation: J Cell Sci. 2003 Apr 1;116(Pt 7):1175-86
references.pmid: 12615961
references.citation: Dev Biol. 2006 Jan 1;289(1):64-76
references.pmid: 16321372
references.citation: Development. 1998 Nov;125(21):4283-92
references.pmid: 9753682
references.citation: Science. 2000 Mar 3;287(5458):1606-9
references.pmid: 10733430
references.citation: Anat Embryol (Berl). 2004 Aug;208(5):333-50
references.pmid: 15309628
references.citation: Dev Dyn. 1997 Aug;209(4):377-86
references.pmid: 9264261
references.citation: Dev Cell. 2004 Jun;6(6):781-90
references.pmid: 15177027
references.citation: Dev Dyn. 1995 Jul;203(3):253-310
references.pmid: 8589427
references.citation: Nat Protoc. 2008;3(1):59-69
references.pmid: 18193022
references.citation: Development. 2004 Feb;131(4):873-80
references.pmid: 14736741
references.citation: Bioessays. 2002 Jul;24(7):613-24
references.pmid: 12111722
references.citation: Development. 1994 Apr;120(4):1009-15
references.pmid: 7600949
references.citation: Development. 2002 Jul;129(13):3137-46
references.pmid: 12070089
references.citation: Development. 2000 May;127(10):2189-99
references.pmid: 10769242
references.citation: Development. 2005 Jun;132(11):2503-12
references.pmid: 15890825
references.citation: Dev Biol. 1997 Mar 1;183(1):108-21
references.pmid: 9119111
references.citation: Dev Biol. 1998 Sep 15;201(2):247-52
references.pmid: 9740662
references.citation: Dev Dyn. 2003 Nov;228(3):535-54
references.pmid: 14579391
references.citation: Development. 1993 Oct;119(2):447-56
references.pmid: 8287796
references.citation: EMBO J. 1998 Oct 1;17(19):5718-33
references.pmid: 9755172
references.citation: Dev Dyn. 2008 Dec;237(12):3715-26
references.pmid: 19035365
references.citation: Gene Expr Patterns. 2006 Oct;6(8):1019-26
references.pmid: 16774848
references.citation: J Anat. 2001 Jul-Aug;199(Pt 1-2):177-80
references.pmid: 11523820
references.citation: Development. 1997 Nov;124(21):4179-91
references.pmid: 9334267