Development of a broadly reactive nested reverse transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence of murine noroviruses in laboratory mice in Japan.
A broadly reactive nested RT-PCR assay to detect MNV was developed and subsequently used to investigate the prevalence of MNV in laboratory mice in Japan. MNV were detected in 8 (22%) of 37 murine stool specimens by second-round PCR, although no positive band was obtained from any specimen by first-round PCR. Genetic analysis of the second round PCR products showed that MNV sequences detected in this study were closely matched (97.2 approximately 99.1%) to that of MNV-3 (DQ223042). This is the first report demonstrating the prevalence of MNV in Japan.
Kitajima
Masaaki
M
Laboratory of Public Health, Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. kitajima@env.t.u-tokyo.ac.jp
Oka
Tomoichiro
T
Tohya
Yukinobu
Y
Katayama
Hiroyuki
H
Takeda
Naokazu
N
Katayama
Kazuhiko
K
D000818:Animals; D000830:Animals, Laboratory / Q000821:virology; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D007564:Japan / Q000453:epidemiology; D051379:Mice; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D048348:Reverse Transcription; D012376:Rodent Diseases / Q000453:epidemiology / Q000821:virology*