[Activated T cells promote differentiation of mesenchymal stem cells into osteoblasts].
This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1beta played an important role in the effect of activated T cells on MSCs, while TNF-alpha, TGF-beta1 were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interactive influence between MSCs and immune cells can be mediated through cytokines.
Liu
Yuan-Lin
YL
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Jiang
Xiao-Xia
XX
Su
Yong-Feng
YF
Huo
Si-Wei
SW
Zhu
Heng
H
Wu
Ying
Y
Li
Xiu-Sen
XS
Mao
Ning
N
Zhang
Yi
Y
D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D013601:T-Lymphocytes / Q000378:metabolism*
D017077:Culture Media, Conditioned; D053583:Interleukin-1beta