Evaluation of potential genotoxicity of virgin olive oil (VOO) using the drosophila wing-spot test.
Edible and nonedible grades of virgin olive oil (VOO), differing in quality characteristics, were evaluated for potential genotoxicity in the Drosophila somatic mutation and recombination test (SMART) before and after heating at high temperatures. Drosophila larvae were fed on medium containing 6 and 12% v/v of each of the examined oils. Edible VOOs did not exhibit any mutagenic or recombination activity even after thermal treatment. Lower grade VOO gave negative results at the concentration of 6% and inconclusive ones at 12%. However, after its thermal treatment, a statistically significant increase of large single spots was observed, giving a positive result for this spot category at both concentrations. Evaluation of the possible contribution of olive phenolic compounds to the nongenotoxic effects observed was carried out using a polar olive leaf extract and pure oleuropein. No significant increase in the frequency of any category of mutant spots was recorded for leaf extract (0.8-12 mg of total polar phenols/dose) or pure oleuropein (0.8-8 mg/dose). These results are expected to contribute to the ongoing interest in the inherent properties of VOO as part of the everyday diet.
Kounatidis
Ilias
I
Department of Genetics, Faculty of Science, School of Biology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Papoti
Vassiliki T
VT
Nenadis
Nikolaos
N
Franzios
Gerasimos
G
Oikonomou
Mariangella
M
Partheniou
Fedra
F
Tsimidou
Maria
M
Mavragani-Tsipidou
Penelope
P
D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D005260:Female; D007814:Larva / Q000187:drug effects / Q000235:genetics; D008297:Male; D016296:Mutagenesis / Q000187:drug effects; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D031658:Olea / Q000737:chemistry; D000069463:Olive Oil; D010936:Plant Extracts / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity*; D011995:Recombination, Genetic / Q000187:drug effects; D014921:Wings, Animal
D009153:Mutagens; D000069463:Olive Oil; D010936:Plant Extracts; D010938:Plant Oils