title: Optical mapping of Langendorff-perfused rat hearts.
abstract: Optical mapping of the cardiac surface with voltage-sensitive fluorescent dyes has become an important tool to investigate electrical excitation in experimental models that range in scale from cell cultures to whole-organs([1, 2]). Using state-of-the-art optical imaging systems, generation and propagation of action potentials during normal cardiac rhythm or throughout initiation and maintenance of arrhythmias can be visualized almost instantly([1]). The latest commercially-available systems can provide information at exceedingly high spatiotemporal resolutions and were based on custom-built equipment initially developed to overcome the obstacles imposed by more conventional electrophysiological methods([1]). Advancements in high-resolution and high-speed complementary metal-oxide-semiconductor (CMOS) cameras and intensely-bright, light-emitting diodes (LEDs) as well as voltage-sensitive dyes, optics, and filters have begun to make electrical signal acquisition practical for cardiovascular cell biologists who are more accustomed to working with microscopes. Although the newest generation of CMOS cameras can acquire 10,000 frames per second on a 16,384 pixel array, depending on the type of sample preparation, long-established fluorescence acquisition technologies such as photodiode arrays, laser scanning systems, and cooled charged-coupled device (CCD) cameras still have some distinct advantages with respect to dynamic range, signal-to-noise ratio, and quantum efficiency([1, 3]). In the present study, Lewis rat hearts were perfused ex vivo with a crystalloid perfusate (Krebs-Henseleit solution) at 37 degrees C on a modified Langendorff apparatus. After a 20 minute stabilization period, the hearts were intermittently perfused with 11 mMol/L 2,3-butanedione monoxime to eliminate contraction-associated motion during image acquisition. For optical mapping, we loaded hearts with the fast-response potentiometric probe di-8-ANEPPS([4]) (5 microMol/L) and briefly illuminated the preparation with 475+/-15 nm excitation light. During a typical 2 second period of illumination, >605 nm light emitted from the cardiac preparation was imaged with a high-speed CMOS camera connected to a horizontal macroscope. For this demonstration, hearts were paced at 300 beats per minute with a coaxial electrode connected to an isolated electrical stimulation unit. Simultaneous bipolar electrographic recordings were acquired and analyzed along with the voltage signals using readily-available software. In this manner, action potentials on the surface of Langendorff-perfused rat hearts can be visualized and registered with electrographic signals.
authors.affiliation: Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston and Harvard Medical School, USA.
authors.forename: Bjoern
authors.initials: B
authors.lastname: Sill
authors.forename: Peter E
authors.initials: PE
authors.lastname: Hammer
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Cowan
mesh_terms: D000818:Animals; D018780:Body Surface Potential Mapping / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006321:Heart / Q000502:physiology*; D006329:Heart Conduction System / Q000502:physiology; D066298:In Vitro Techniques; D010477:Perfusion; D011726:Pyridinium Compounds / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds
references.citation: Circ Res. 2004 Jul 9;95(1):21-33
references.pmid: 15242982
references.citation: IEEE Trans Biomed Eng. 2004 Feb;51(2):333-41
references.pmid: 14765706
references.citation: Prog Biophys Mol Biol. 2006 Oct;92(2):232-57
references.pmid: 16330086
references.citation: Heart Rhythm. 2007 May;4(5):619-26
references.pmid: 17467631
references.citation: Am J Pathol. 2006 Jul;169(1):72-85
references.pmid: 16816362
references.citation: Anesthesiology. 2001 Dec;95(6):1396-405
references.pmid: 11748398
references.citation: J Pharmacol Toxicol Methods. 2007 Mar-Apr;55(2):113-26
references.pmid: 16844390
references.citation: Circulation. 2008 Mar 18;117(11):1474-7
references.pmid: 18347223