title: Silicon deprivation does not significantly modify the acute white blood cell response but does modify tissue mineral distribution response to an endotoxin challenge.
abstract: An experiment with rats was conducted to determine whether silicon deprivation affects the acute-phase immune response to an endotoxin challenge. Weanling female rats were assigned to two weight-matched groups of 24; one group was fed a basal diet containing about 1.9 microg Si/kg; the other group was fed the basal diet supplemented with 35 microg Si/kg as arginine silicate inositol complex. After being fed their respective diets for 8 weeks, 12 rats in each group were injected subcutaneously with 1 mg lipopolysaccharide (LPS)/kg body weight; the other 12 rats in each group were injected with deionized water. Two hours after injection, the rats were anesthetized with ether for collection of blood (for plasma), liver and femurs, and then euthanized by decapitation. LPS injection decreased total white blood cell, lymphocyte, monocyte, eosinophil, and basophil counts by 80-90%, but did not affect neutrophil counts. LPS injection also increased plasma tumor necrosis factor-alpha and osteopontin and decreased plasma hyaluronic acid. Silicon deprivation did not significantly affect any of these responses to LPS. Silicon in liver and silicon, iron, and zinc in femur were increased by LPS injection only in silicon-deprived rats. Silicon deprivation also increased monocyte counts and osteopontin and decreased femur zinc in rats not injected with LPS. The findings indicate that silicon deprivation does not affect the acute-immune phase decrease in inflammatory cell numbers and increase in inflammatory cytokines in response to an endotoxin challenge. Silicon deprivation, however, apparently causes slight chronic inflammation and might influence inflammatory cell proliferation in the chronic-phase inflammatory response.
authors.affiliation: Grand Forks Human Nutrition Research Center, ARS, USDA, 2420 2nd Ave North Stop 9034, Grand Forks, ND 58202-9034, USA. forrest.nielsen@ars.usda.gov
authors.forename: Forrest H
authors.initials: FH
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage*; D015519:Bone Density / Q000187:drug effects*; D004731:Endotoxins / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D005269:Femur / Q000187:drug effects / Q000276:immunology; D007249:Inflammation / Q000276:immunology; D007294:Inositol / Q000008:administration & dosage*; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology / Q000633:toxicity*; D008099:Liver / Q000276:immunology; D053495:Osteopontin / Q000097:blood; D010976:Platelet Count; D051381:Rats; D017207:Rats, Sprague-Dawley; D017640:Silicates / Q000008:administration & dosage*
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D017640:Silicates; C506897:Spp1 protein, rat; D053495:Osteopontin; D007294:Inositol; D001120:Arginine