title: Kinetic analysis of amyloid formation in the presence of heparan sulfate: faster unfolding and change of pathway.
abstract: A number of human diseases are associated with the conversion of proteins from their native state into well defined fibrillar aggregates, depositing in the extracellular space and generally termed amyloid fibrils. Heparan sulfate (HS), a glycosaminoglycan normally present in the extracellular matrix, has been found to be universally associated with amyloid deposits and to promote amyloid fibril formation by all studied protein systems. We have studied the impact of HS on the amyloidogenesis of human muscle acylphosphatase, monitoring the process with an array of techniques, such as normal and stopped-flow far-UV circular dichroism, thioflavin T fluorescence, static and dynamic light scattering, and atomic force microscopy. The results show that HS accelerates the conversion of the studied protein from the native state into the amyloidogenic, yet monomeric, partially folded state. They also indicate that HS does not simply accelerate the conversion of the resulting partially folded state into amyloid species but splits the process into two distinct pathways occurring in parallel: a very fast phase in which HS interacts with a fraction of protein molecules, causing their rapid aggregation into ThT-positive and beta-sheet containing oligomers, and a slow phase resulting from the normal aggregation of partially folded molecules that cannot interact with HS. The HS-mediated aggregation pathway is severalfold faster than that observed in the absence of HS. Two aggregation phases are generally observed when proteins aggregate in the presence of HS, underlying the importance of a detailed kinetic analysis to fully understand the effect of this glycosaminoglycan on amyloidogenesis.
authors.affiliation: Department of Biochemistry, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors.forename: Neda
authors.initials: N
authors.lastname: Motamedi-Shad
authors.forename: Elodie
authors.initials: E
authors.lastname: Monsellier
authors.forename: Silvia
authors.initials: S
authors.lastname: Torrassa
authors.forename: Annalisa
authors.initials: A
authors.lastname: Relini
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Chiti
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000737:chemistry* / Q000378:metabolism; D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism; D052160:Benzothiazoles; D002942:Circular Dichroism; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D006497:Heparan Sulfate / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009124:Muscle Proteins / Q000737:chemistry* / Q000378:metabolism; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D013844:Thiazoles / Q000737:chemistry*; D000097024:Acylphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D000682:Amyloid; D052160:Benzothiazoles; D006497:Heparan Sulfate; D009124:Muscle Proteins; D013844:Thiazoles; D000097024:Acylphosphatase; C009462:thioflavin T
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