title: The conserved metalloprotease invadolysin localizes to the surface of lipid droplets.
abstract: Invadolysin is a metalloprotease conserved in many different organisms, previously shown to be essential in Drosophila with roles in cell division and cell migration. The gene seems to be ubiquitously expressed and four distinct splice variants have been identified in human cells but not in most other species examined. Immunofluorescent detection of human invadolysin in cultured cells reveals the protein to be associated with the surface of lipid droplets. By means of subcellular fractionation, we have independently confirmed the association of invadolysin with lipid droplets. We thus identify invadolysin as the first metalloprotease located on these dynamic organelles. In addition, analysis of larval fat-body morphological appearance and triglyceride levels in the Drosophila invadolysin mutant suggests that invadolysin plays a role in lipid storage or metabolism.
authors.affiliation: University of Edinburgh, Queen's Medical Research Institute, Centre for Cardiovascular Science, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.
authors.forename: Neville
authors.initials: N
authors.lastname: Cobbe
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Marshall
authors.forename: Shubha
authors.initials: S
authors.lastname: Gururaja Rao
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Chang
authors.forename: Francesca
authors.initials: F
authors.lastname: Di Cara
authors.forename: Edward
authors.initials: E
authors.lastname: Duca
authors.forename: Sharron
authors.initials: S
authors.lastname: Vass
authors.forename: Adam
authors.initials: A
authors.lastname: Kassan
authors.forename: Margarete M S
authors.initials: MM
authors.lastname: Heck
mesh_terms: D017398:Alternative Splicing / Q000187:drug effects / Q000235:genetics; D000818:Animals; D002460:Cell Line; D017124:Conserved Sequence; D004330:Drosophila / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology; D010802:Phylogeny; D021381:Protein Transport / Q000187:drug effects; D011554:Pseudopodia / Q000187:drug effects / Q000201:enzymology
chemical_list: D029721:Drosophila Proteins; D008055:Lipids; D019301:Oleic Acid; D008666:Metalloendopeptidases; C495212:invadolysin, Drosophila; C495213:invadolysin, human
references.citation: J Cell Biol. 1998 May 18;141(4):905-15
references.pmid: 9585410
references.citation: Trends Cell Biol. 2007 Aug;17(8):363-9
references.pmid: 17766117
references.citation: Bioinformatics. 2002 Mar;18(3):502-4
references.pmid: 11934758
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):3825-32
references.pmid: 1069267
references.citation: Clin Cancer Res. 2007 Jan 1;13(1):152-60
references.pmid: 17200350
references.citation: J Immunol. 2004 Jul 1;173(1):559-65
references.pmid: 15210817
references.citation: Exp Cell Res. 1999 May 25;249(1):33-45
references.pmid: 10328951
references.citation: Mol Biochem Parasitol. 2003 Nov;132(1):1-16
references.pmid: 14563532
references.citation: Cell. 1996 Sep 20;86(6):849-52
references.pmid: 8808619
references.citation: Int J Parasitol. 2001 Jul;31(9):899-908
references.pmid: 11406139
references.citation: Curr Biol. 2006 Sep 19;16(18):1783-95
references.pmid: 16979555
references.citation: Acta Trop. 1992 Feb;50(3):205-17
references.pmid: 1348597
references.citation: Science. 2005 Jul 15;309(5733):436-42
references.pmid: 16020728
references.citation: Histochem Cell Biol. 2005 Nov;124(5):445-52
references.pmid: 16151827
references.citation: Mech Dev. 2003 Sep;120(9):1071-81
references.pmid: 14550535
references.citation: Science. 2006 Jun 30;312(5782):1868-9
references.pmid: 16809506
references.citation: Nature. 2008 Aug 21;454(7207):955-60
references.pmid: 18719581
references.citation: Int Immunopharmacol. 2008 Oct;8(10):1308-15
references.pmid: 18687292
references.citation: J Bacteriol. 2005 Jun;187(11):3607-19
references.pmid: 15901682
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Eur J Cell Biol. 2006 Dec;85(12):1217-31
references.pmid: 17010475
references.citation: Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W244-8
references.pmid: 15980461
references.citation: Planta. 2007 Jul;226(2):405-16
references.pmid: 17318608
references.citation: J Mol Biol. 2000 Sep 8;302(1):205-17
references.pmid: 10964570
references.citation: Eur J Cell Biol. 2008 Sep;87(8-9):641-7
references.pmid: 18375013
references.citation: J Cell Biol. 2001 Mar 5;152(5):1079-85
references.pmid: 11238462
references.citation: Proc Natl Acad Sci U S A. 1992 May 15;89(10):4693-7
references.pmid: 1584806
references.citation: Mol Biol Cell. 2006 Jun;17(6):2674-83
references.pmid: 16597703
references.citation: Curr Opin Cell Biol. 2008 Aug;20(4):378-85
references.pmid: 18606534
references.citation: PLoS Genet. 2007 Nov;3(11):e199
references.pmid: 18081423
references.citation: Biochem Biophys Res Commun. 2007 Jun 8;357(3):792-9
references.pmid: 17451652
references.citation: J Cell Biol. 2001 Mar 5;152(5):1071-8
references.pmid: 11238461
references.citation: Biochemistry. 2008 Feb 5;47(5):1274-87
references.pmid: 18186652
references.citation: Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):94-9
references.pmid: 18160536
references.citation: Mol Biol Cell. 2004 Jan;15(1):99-110
references.pmid: 14528016
references.citation: Cancer Res. 2008 Mar 15;68(6):1732-40
references.pmid: 18339853
references.citation: Am J Respir Cell Mol Biol. 2007 Nov;37(5):562-70
references.pmid: 17585108
references.citation: Nat Cell Biol. 2007 Sep;9(9):1089-97
references.pmid: 17721513
references.citation: J Cell Biol. 2004 Nov 22;167(4):673-86
references.pmid: 15557119
references.citation: J Cell Sci. 2005 May 15;118(Pt 10):2079-82
references.pmid: 15890982
references.citation: Trends Microbiol. 2006 Jan;14(1):8-11
references.pmid: 16356717
references.citation: Mol Biol Evol. 2001 May;18(5):691-9
references.pmid: 11319253
references.citation: J Biol Chem. 1997 Apr 4;272(14):9378-87
references.pmid: 9083075
references.citation: Biochem J. 1993 Sep 1;294 ( Pt 2):305-24
references.pmid: 8373346
references.citation: FEBS Lett. 2006 Oct 9;580(23):5484-91
references.pmid: 16962104
references.citation: J Biol Chem. 2003 May 2;278(18):15998-6007
references.pmid: 12591929
references.citation: Eur J Cell Biol. 2006 Apr;85(3-4):159-64
references.pmid: 16546558
references.citation: Trends Genet. 2004 Jul;20(7):273-7
references.pmid: 15219388
references.citation: Mol Biotechnol. 2003 Jun;24(2):157-202
references.pmid: 12746556
references.citation: EMBO Rep. 2006 Jan;7(1):106-13
references.pmid: 16239926
references.citation: Structure. 1998 Aug 15;6(8):1035-46
references.pmid: 9739094
references.citation: Anal Biochem. 1993 Apr;210(1):179-87
references.pmid: 8489015
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10159-64
references.pmid: 16002471
references.citation: Mol Biol Cell. 2003 Mar;14(3):1074-84
references.pmid: 12631724
references.citation: J Cell Sci. 2003 Mar 1;116(Pt 5):847-55
references.pmid: 12571282
references.citation: Curr Biol. 2003 Apr 1;13(7):603-6
references.pmid: 12676093
references.citation: Prog Lipid Res. 2001 Sep;40(5):325-438
references.pmid: 11470496
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: Cell. 1992 Feb 21;68(4):673-82
references.pmid: 1739974
references.citation: Nat Rev Mol Cell Biol. 2002 Jul;3(7):509-19
references.pmid: 12094217
references.citation: Eur J Biochem. 2001 Aug;268(15):4217-26
references.pmid: 11488915
references.citation: Nature. 2008 Feb 14;451(7180):783-8
references.pmid: 18273011