title: [Effects of 8-Methoxypsoralen on intracellular [Ca(2+)]i and cytoskeleton actin organization in human melanocytes in vitro].
abstract: OBJECTIVE
  To investigate the effects of 8-methoxypsoralen on human melanocytes [Ca(2+)]i and cytoskeleton actin organization in vitro.
  
  
  METHODS
  Human melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca(2+)]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin.
  
  
  RESULTS
  8-methoxypsoralen increased [Ca(2+)]i and induced organization of actin stress fiber cytoskeleton.
  
  
  CONCLUSIONS
  8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca(2+) concentration and cytoskeleton actin reorganization.
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Xian-qi
authors.initials: XQ
authors.lastname: ZHANG
authors.forename: Min
authors.initials: M
authors.lastname: ZHENG
authors.forename: Kuan-hou
authors.initials: KH
authors.lastname: MOU
authors.forename: Jie
authors.initials: J
authors.lastname: FENG
mesh_terms: D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008730:Methoxsalen / Q000494:pharmacology*; D012867:Skin / Q000166:cytology
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D002118:Calcium; D008730:Methoxsalen