title: The presence of kinesin superfamily motor proteins KIFC1 and KIF17 in normal and pathological human placenta.
abstract: Kinesin superfamily proteins (KIFs) are motor proteins that participate in chromosomal and spindle movements during mitosis and meiosis, and transport membranous organelles and macromolecules fundamental for cellular functions. Although the roles of KIFs in axonal and dendritic transports have been studied extensively, their role in intracellular transport in general is less well known. The diversity of kinesins suggests that each kinesin may have a specific function. Therefore, in this study we aimed to investigate the presence and cellular localization of KIFC1 and KIF17 in normal and pathological human placentas. First-trimester (22-56 days) and normal, preeclamptic (PE), and diabetic-term placental tissues were obtained and further studied by immunohistochemistry (IHC) and Western blot methods. KIFC1 was mainly localized to the syncytiotrophoblast both in early and term placental samples. However, a stronger immunoreactivity was observed both in PE and diabetic placentas compared to normal-term placentas. KIF17 was most intensively localized in developing vascular endothelium in early pregnancy. Even though KIF17 was moderately stained in the endothelium of villi from normal human-term placentas, stronger immunoreactivity was observed in all types of villi of both PE and diabetic placentas. Western blotting of tissue extracts confirmed the IHC results. Here, we demonstrate the presence of KIFC1 and KIF17 in human placenta for the first time. The intense expression of KIFC1 in syncytiotrophoblast and KIF17 in vascular endothelium suggests that both the proteins might be important in a cargo-transport system. An increased expression pattern of both KIFC1 and KIF17 in PE and diabetes might suggest that these proteins may be involved in complex trophoblast functions and placental pathologies. Further studies will clarify the physiological role of KIFs in human placental transport and development.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: L
authors.initials: L
authors.lastname: Sati
authors.forename: Y
authors.initials: Y
authors.lastname: Seval-Celik
authors.forename: G
authors.initials: G
authors.lastname: Unek
authors.forename: E T
authors.initials: ET
authors.lastname: Korgun
authors.forename: R
authors.initials: R
authors.lastname: Demir
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D002824:Chorionic Villi / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D010920:Placenta / Q000166:cytology / Q000378:metabolism* / Q000473:pathology*; D010929:Placentation; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism; D011254:Pregnancy in Diabetics / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism; D013997:Time Factors; D014327:Trophoblasts / Q000378:metabolism
chemical_list: C485813:KIF17 protein, human; C486566:KIFC1 protein, human; D016547:Kinesins