PTEN expression is down-regulated in liver tissues of rats with hepatic fibrosis induced by biliary stenosis.
The gene phosphatase and tensin homolog deleted on chromosome 10 (PTEN) codes for a tumor-suppressor phospholipid phosphatase. Deletion, mutation or abnormal expression of PTEN is commonly found in many kinds of malignant tumors. At the time of this study, though, the role of PTEN expression in the pathology of hepatic fibrosis remains unclear. In this study, we investigate the dynamic expression of PTEN in a rat model of hepatic fibrosis, with special emphasis on the activation and proliferation of hepatic stellate cells (HSC) in vivo. The rat model of hepatic fibrosis used in this study employed common bile duct ligation. At four time points, the expression of PTEN in hepatic tissues and activated HSC in rat liver tissues was measured by immunohistochemical staining, Western blotting, real-time fluorescent quantitative PCR and immunofluorescence confocal laser scanning microscopy, respectively. Further, alpha-smooth muscle actin (alpha-SMA), an activated HSC marker in rat liver tissues, was detected by immunohistochemical staining. This study showed that aggravation of hepatic fibrosis led to gradually decreasing expression of PTEN in the hepatic tissues. Further, as hepatic fibrosis worsens, PTEN-expressing activated HSC accounts for an increasingly smaller percentage of all activated HSC. In contrast, the percentage of alpha-SMA-expressing HSC cells increases significantly. In conclusion, expression of PTEN mRNA and protein is down-regulated in fibrogenic rat liver tissue, and its expression in HSC in vivo also decreases with progression of fibrosis. Thus, these results show that the dynamic expression of PTEN in hepatic tissues negatively correlates with activation and proliferation of HSC.
Hao
Li Sen
LS
Department of Gastroenterology, The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, Shijiazhuang, Hebei Province, China.
Zhang
Xiao Lan
XL
An
Jun Yan
JY
Karlin
Justin
J
Tian
Xiao Peng
XP
Dun
Zhi Na
ZN
Xie
Shu Rui
SR
Chen
Shuang
S
D000199:Actins / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D049109:Cell Proliferation; D003135:Common Bile Duct; D017931:DNA Primers / Q000235:genetics; D015536:Down-Regulation; D055166:Hepatic Stellate Cells / Q000378:metabolism / Q000473:pathology; D008026:Ligation; D008106:Liver Cirrhosis, Experimental / Q000209:etiology / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000199:Actins; D017931:DNA Primers; D051059:PTEN Phosphohydrolase; D012333:RNA, Messenger; C498862:Acta2 protein, rat; C487216:Pten protein, rat