Effect of physical parameters on the main phase transition of supported lipid bilayers.
Supported lipid bilayers composed of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and 1-palmitoyl-2-oleoyl-phosphatidylglycerol (POPG) were assembled by the vesicle fusion technique on mica and studied by temperature-controlled atomic force microscopy. The role of different physical parameters on the main phase transition was elucidated. Both mixed (POPE/POPG 3:1) and pure POPE bilayers were studied. By increasing the ionic strength of the solution and the incubation temperature, a shift from a decoupled phase transition of the two leaflets, to a coupled transition, with domains in register, was obtained. The observed behavior points to a modulation of the substrate/bilayer and interleaflet coupling induced by the environment and preparation conditions of supported lipid bilayers. The results are discussed in view of the role of different interactions in the system. The influence of the substrate on the lipid bilayers, in terms of interleaflet coupling, can also help us in understanding the possible effect that submembrane elements like the cytoskeleton might have on the structure and dynamics of biomembranes.
Seeger
H M
HM
CNR-INFM-S3 National Center on Nanostructure and BioSystems at Surfaces, Modena, Italy.
Marino
G
G
Alessandrini
A
A
Facci
P
P
D003198:Computer Simulation; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*
D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; C057561:1-palmitoyl-2-oleoylphosphatidylethanolamine; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol
Biophys J. 1991 Feb;59(2):289-94
2009353
Biophys J. 2005 Mar;88(3):2154-64
15596519
Biophys J. 2004 Jun;86(6):3783-93
15189874
Langmuir. 2005 Feb 15;21(4):1377-88
15697284
Biophys J. 1997 Apr;72(4):1659-67
9083669
Biophys J. 1990 Aug;58(2):357-62
2207243
Mol Membr Biol. 2006 Jan-Feb;23(1):17-28
16600898
Biochim Biophys Acta. 1977 Oct 17;470(2):185-201
911827
Biophys J. 2003 Jul;85(1):350-60
12829489
Biophys J. 2006 Nov 1;91(9):3313-26
16905614
Methods Enzymol. 1998;295:468-94
9750233
Biophys J. 2005 Aug;89(2):1094-101
15879467
Biophys J. 2004 May;86(5):2942-50
15111410
Biophys J. 1998 Sep;75(3):1397-1402
9726940
Surf Sci Rep. 2006 Nov 15;61(10):429-444
32287559
Langmuir. 2007 May 22;23(11):5886-95
17428076
Biophys J. 2007 Feb 15;92(4):1263-70
17114215
Biophys J. 2007 Dec 15;93(12):4268-77
17766349
Biophys J. 1999 May;76(5):2600-5
10233074
Ultramicroscopy. 2003 Oct-Nov;97(1-4):217-27
12801674
Proc Natl Acad Sci U S A. 1984 Oct;81(19):6159-63
6333027
Traffic. 2004 Apr;5(4):241-6
15030565
Biophys J. 2003 Nov;85(5):3035-47
14581204
Phys Rev Lett. 2005 Jun 17;94(23):238104
16090507
Biochim Biophys Acta. 2009 Jan;1788(1):64-71
18848518
Langmuir. 2006 Dec 5;22(25):10777-83
17129059
Phys Rev Lett. 2005 Jan 21;94(2):025701
15698195
Biochemistry. 1994 Nov 29;33(47):14091-9
7947819
Chemphyschem. 2006 Jun 12;7(6):1207-10
16676368
Biochim Biophys Acta. 2005 Oct 1;1716(1):40-8
16150420
Biophys J. 2000 Jul;79(1):434-47
10866969
Langmuir. 2007 Jul 3;23(14):7644-51
17539662
Cell Biochem Biophys. 2004;41(3):391-414
15509889
Langmuir. 2008 Feb 19;24(4):1219-24
18062709
Biophys J. 1999 Sep;77(3):1445-57
10465755
Langmuir. 2005 Jan 4;21(1):299-304
15620318
Science. 1996 Jan 5;271(5245):43-8
8539599
Biochim Biophys Acta. 1986 Jun 12;864(1):95-106
2941079
Biophys J. 2008 Mar 1;94(5):L32-4
18096628
J Lipid Res. 2003 Feb;44(2):233-42
12576505
Annu Rev Cell Dev Biol. 1998;14:111-36
9891780
Biophys J. 1985 Jan;47(1):105-13
3978184
Nature. 2005 Sep 29;437(7059):656-63
16193040
J Electron Microsc (Tokyo). 2002;51(1):1-9
12003236
Nat Cell Biol. 2007 Jan;9(1):7-14
17199125
Biophys J. 1996 Mar;70(3):1419-31
8785298
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):124-8
18172219
Biophys J. 2006 Jan 1;90(1):228-37
16214871