title: Demonstrated and inferred metabolism associated with cytosolic lipid droplets.
abstract: Cytosolic lipid droplets were considered until recently to be rather inert particles of stored neutral lipid. Largely through proteomics is it now known that droplets are dynamic organelles and that they participate in several important metabolic reactions as well as trafficking and interorganellar communication. In this review, the role of droplets in metabolism in the yeast Saccharomyces cerevisiae, the fly Drosophila melanogaster, and several mammalian sources are discussed, particularly focusing on those reactions shared by these organisms. From proteomics and older work, it is clear that droplets are important for fatty acid and sterol biosynthesis, fatty acid activation, and lipolysis. However, many droplet-associated enzymes are predicted to span a membrane two or more times, which suggests either that droplet structure is more complex than the current model posits, or that there are tightly bound membranes, particularly derived from the endoplasmic reticulum, which account for the association of several of these proteins.
authors.affiliation: Department of Pharmacology University of Texas Southwestern Medical School Dallas TX 75390-9041, USA. Joel.Goodman@UTSouthwestern.edu
authors.forename: Joel M
authors.initials: JM
authors.lastname: Goodman
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D008066:Lipolysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis
chemical_list: D005227:Fatty Acids; D008055:Lipids; D013261:Sterols
references.citation: J Biol Chem. 2006 Jan 6;281(1):491-500
references.pmid: 16267052
references.citation: J Lipid Res. 2007 Nov;48(11):2354-64
references.pmid: 17679730
references.citation: J Biol Chem. 2004 Nov 19;279(47):48968-75
references.pmid: 15364929
references.citation: J Biol Chem. 2004 Nov 5;279(45):46835-42
references.pmid: 15337753
references.citation: J Biol Chem. 2008 Sep 5;283(36):24525-33
references.pmid: 18606822
references.citation: Curr Biol. 2006 Sep 19;16(18):1783-95
references.pmid: 16979555
references.citation: FEMS Yeast Res. 2007 Jun;7(4):629-38
references.pmid: 17419771
references.citation: Biochemistry. 2005 Feb 8;44(5):1635-42
references.pmid: 15683247
references.citation: Nature. 2003 Oct 16;425(6959):686-91
references.pmid: 14562095
references.citation: Mol Biol Cell. 1998 Feb;9(2):375-86
references.pmid: 9450962
references.citation: Biochim Biophys Acta. 2009 Jun;1791(6):419-40
references.pmid: 19375517
references.citation: Biochem Biophys Res Commun. 1980 Sep 16;96(1):522-8
references.pmid: 6776955
references.citation: Trends Cell Biol. 2009 Feb;19(2):81-8
references.pmid: 19144519
references.citation: J Lipid Res. 1998 Sep;39(9):1889-95
references.pmid: 9741702
references.citation: J Biochem. 2006 May;139(5):921-30
references.pmid: 16751600
references.citation: Electrophoresis. 2000 Oct;21(16):3470-82
references.pmid: 11079566
references.citation: Adv Exp Med Biol. 1998;441:157-70
references.pmid: 9781323
references.citation: Mol Endocrinol. 2003 Sep;17(9):1715-25
references.pmid: 12829805
references.citation: Traffic. 2006 Sep;7(9):1254-69
references.pmid: 17004324
references.citation: Biochem Biophys Res Commun. 2007 May 11;356(3):792-8
references.pmid: 17382292
references.citation: Int Arch Allergy Immunol. 1994 Nov;105(3):245-50
references.pmid: 7920026
references.citation: J Biol Chem. 2004 May 28;279(22):23699-709
references.pmid: 15024010
references.citation: J Biol Chem. 2004 Jan 30;279(5):3787-92
references.pmid: 14597625
references.citation: J Proteome Res. 2007 Aug;6(8):3256-65
references.pmid: 17608402
references.citation: Eur J Biochem. 2004 Oct;271(20):4141-6
references.pmid: 15479243
references.citation: Endocrinology. 2003 May;144(5):2084-91
references.pmid: 12697717
references.citation: Traffic. 2008 Mar;9(3):338-52
references.pmid: 18088320
references.citation: Arch Biochem Biophys. 2002 Aug 15;404(2):263-70
references.pmid: 12147264
references.citation: J Biol Chem. 2000 Jan 7;275(1):235-40
references.pmid: 10617610
references.citation: J Bacteriol. 2002 Jan;184(2):519-24
references.pmid: 11751830
references.citation: Biochem Biophys Res Commun. 1997 Apr 7;233(1):253-7
references.pmid: 9144433
references.citation: J Biol Chem. 2005 Nov 11;280(45):37301-9
references.pmid: 16135509
references.citation: Hum Mol Genet. 2003 Nov 15;12(22):2981-91
references.pmid: 14506130
references.citation: FEBS J. 2007 Sep;274(18):4837-47
references.pmid: 17714510
references.citation: J Biol Chem. 2006 Feb 17;281(7):4446-56
references.pmid: 16361250
references.citation: J Biol Chem. 1991 Jun 15;266(17):11341-6
references.pmid: 2040638
references.citation: J Biol Chem. 1970 Apr 10;245(7):1670-4
references.pmid: 5438357
references.citation: J Lipid Res. 2007 Dec;48(12):2547-59
references.pmid: 17878492
references.citation: J Biol Chem. 2009 Jul 3;284(27):18347-56
references.pmid: 19429676
references.citation: J Biol Chem. 2002 Jan 25;277(4):2406-12
references.pmid: 11706015
references.citation: Exp Biol Med (Maywood). 2008 May;233(5):507-21
references.pmid: 18375835
references.citation: J Biol Chem. 2008 Jun 20;283(25):17065-74
references.pmid: 18430725
references.citation: J Biol Chem. 2006 Apr 28;281(17):11901-9
references.pmid: 16488886
references.citation: J Bacteriol. 1997 Dec;179(24):7611-6
references.pmid: 9401016
references.citation: Eur J Cell Biol. 1997 Dec;74(4):399-406
references.pmid: 9438137
references.citation: Arch Biochem Biophys. 2008 Nov 15;479(2):121-30
references.pmid: 18804447
references.citation: Curr Opin Lipidol. 2003 Jun;14(3):289-97
references.pmid: 12840660
references.citation: Biochim Biophys Acta. 2003 Jul 4;1633(1):68-74
references.pmid: 12842197
references.citation: J Lipid Res. 2006 Mar;47(3):665-72
references.pmid: 16357361
references.citation: FEBS Lett. 2006 Oct 9;580(23):5484-91
references.pmid: 16962104
references.citation: Biochim Biophys Acta. 2009 Jun;1791(6):408-18
references.pmid: 19118639
references.citation: Biochem Soc Trans. 2003 Dec;31(Pt 6):1120-4
references.pmid: 14641008
references.citation: Mol Cell Proteomics. 2005 May;4(5):662-72
references.pmid: 15716577
references.citation: Nat Rev Mol Cell Biol. 2006 May;7(5):373-8
references.pmid: 16550215
references.citation: Biochim Biophys Acta. 2004 Feb 2;1644(1):47-59
references.pmid: 14741744
references.citation: J Biol Chem. 2006 Apr 7;281(14):9210-8
references.pmid: 16467296
references.citation: Biochim Biophys Acta. 2007 Jun;1773(6):784-93
references.pmid: 17395284
references.citation: J Biol Chem. 2006 Dec 29;281(52):40273-82
references.pmid: 17035227
references.citation: Cell Metab. 2006 May;3(5):309-19
references.pmid: 16679289
references.citation: J Biol Chem. 2002 Aug 23;277(34):31062-71
references.pmid: 12052836
references.citation: J Cell Sci. 2008 Jun 1;121(11):1852-60
references.pmid: 18477614
references.citation: Mamm Genome. 2001 Sep;12(9):741-9
references.pmid: 11641724
references.citation: J Cell Biol. 2006 Jun 5;173(5):719-31
references.pmid: 16735577
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20890-5
references.pmid: 18093937
references.citation: J Bacteriol. 1999 Oct;181(20):6441-8
references.pmid: 10515935
references.citation: Curr Biol. 2005 Jul 26;15(14):1266-75
references.pmid: 16051169
references.citation: J Biol Chem. 2004 Nov 5;279(45):47066-75
references.pmid: 15337759
references.citation: Mol Cell Biol. 2005 Mar;25(5):1655-68
references.pmid: 15713625
references.citation: J Proteome Res. 2006 Jul;5(7):1543-54
references.pmid: 16823961
references.citation: Mol Cell. 2009 Jan 16;33(1):53-63
references.pmid: 19150427
references.citation: FASEB J. 2007 Jan;21(1):167-78
references.pmid: 17135363
references.citation: Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2244-9
references.pmid: 11226224
references.citation: J Biol Chem. 1964 Feb;239:401-9
references.pmid: 14169138
references.citation: Science. 2004 Nov 19;306(5700):1383-6
references.pmid: 15550674
references.citation: Biochim Biophys Acta. 2007 Mar;1771(3):255-70
references.pmid: 16950653
references.citation: J Biol Chem. 1980 Dec 10;255(23):11357-64
references.pmid: 7440546
references.citation: Mol Cell Proteomics. 2006 Jun;5(6):1082-94
references.pmid: 16543254
references.citation: Trends Endocrinol Metab. 2008 Jan;19(1):3-9
references.pmid: 18155916
references.citation: J Lipid Res. 2009 Jan;50(1):3-21
references.pmid: 18952573