title: Control of MRSA infection and colonisation in an intensive care unit by GeneOhm MRSA assay and culture methods.
abstract: BACKGROUND
  Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major nosocomial pathogens. Due to the diffusion of MRSA strains in both hospital and community settings, prevention and control strategies are receiving increased attention. Approximately 25% to 30% of the population is colonised with S. aureus and 0.2% to 7% with MRSA. The BD GeneOhm MRSA real-time PCR assay offers quicker identification of MRSA-colonised patients than do culture methods.
  
  
  METHODS
  Ninety-five patients admitted to the Intensive Care Unit of IRCCS Policlinico San Matteo of Pavia (Italy) for a period > 24 h were screened for MRSA colonisation with both the culture method and the GeneOhm assay.
  
  
  RESULTS
  Of the 246 nasal swabs collected from 95 patients, 36 samples were found to be positive by both methods (true-positive). 30% of colonised patients had developed the MRSA infection.
  
  
  CONCLUSIONS
  Our results show that the GeneOhm MRSA assay is a valuable diagnostic tool for detecting MRSA quickly in nasal swabs. This study confirms that colonisation represents a high risk factor for MRSA infection, and that good MRSA surveillance in an Intensive Care Unit is therefore an excellent way to prevent MRSA infection.
authors.affiliation: Laboratorio di Batteriologia e Micologia, Area Infettivologica, Fondazione IRCCS S, Matteo, Pavia, Italia. claudia_dallavalle01@yahoo.it
authors.forename: Claudia
authors.initials: C
authors.lastname: Dalla Valle
authors.forename: Maria Rosalia
authors.initials: MR
authors.lastname: Pasca
authors.forename: Debora
authors.initials: D
authors.lastname: De Vitis
authors.forename: Federico Capra
authors.initials: FC
authors.lastname: Marzani
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Emmi
authors.forename: Piero
authors.initials: P
authors.lastname: Marone
mesh_terms: D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D017053:Infection Control / Q000379:methods*; D007362:Intensive Care Units; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D009296:Nasal Cavity / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control*
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