Hospital-based prevalence and sensitivity of high-risk human papillomavirus in Thai urban population.
BACKGROUND
To study the prevalence of high-risk human papillomavirus (HPV) types 16, 18, 31 and 33 in healthy Thai women using polymerase chain reaction (PCR) technique.


METHODS
Two hundred and sixty three healthy urban women in Pathumthani, Thailand were recruited. Cervical cancer screening was performed and residual specimen from Pap smears was subjected to PCR to identify the presence of HPV types 16, 18, 31 and 33. Individuals' demographic, health-specific and sexual behavior data were also collected.


RESULTS
Colposcopic biopsy revealed cases with high-grade squamous intraepithelial lesions (HSIL) and low-grade squamous intraepithelial lesions (LSIL). HPV 16 and 18 positive results were associated with abnormal Pap smears. Genotyping gave a 6.1%, 11.8%, 12.1%, and 14.1%, prevalence for HPV types 16, 18, 31 and 33, while 25% were infected with multiple HPV types.


CONCLUSIONS
High-risk HPV screening, used with abnormal pathology of HSIL gives 100% sensitivity and negative predictive value. Data from patient showed not significant correlation with neither different religion level of education, marital status, age of first sexual experience nor the number of sexual partners. Thus high-risk HPV screening is a recommended procedure with excellent sensitivity for detecting HSIL.
Suwannarurk
Komsun
K
Department of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, Thailand. k_suwannarurk@yahoo.com
Tapanadechopol
Pairath
P
Pattaraarchachai
Junya
J
Bhamarapravati
Sutatip
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification / Q000302:isolation & purification*; D003127:Colposcopy; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D008403:Mass Screening; D008875:Middle Aged; D065006:Papanicolaou Test; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D015995:Prevalence; D012680:Sensitivity and Specificity; D012725:Sexual Behavior; D013785:Thailand / Q000453:epidemiology; D014505:Urban Population / Q000706:statistics & numerical data; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult
D004279:DNA, Viral