Molecular cloning and characterization of LPS-binding protein/bactericidal permeability-increasing protein (LBP/BPI) from olive flounder, Paralichthys olivaceus.
A lipopolysaccharide (LPS)-binding protein/bactericidal permeability-increasing protein (LBP/BPI) homolog was isolated from peripheral blood leukocytes cDNA library of olive flounder Paralichthys olivaceus. The isolated LBP/BPI cDNA is 2806bp in length with a 1419bp open reading frame (ORF) that encodes a protein of 472 amino acid residues. The LPS-binding domain is well conserved in the N-terminal barrel, showing high sequence identities with other teleost LBP/BPI as well as those of mammals. RT-PCR analysis revealed that mRNA expression of LBP/BPI was significantly elevated in all tested tissues (liver, gill, intestine, head kidney, and spleen) after intraperitoneal injection of the gram-negative bacterium Edwardsiella tarda or the gram-positive bacterium Streptococcus iniae. This expression pattern profile corresponded to that of acute inflammatory cytokines, suggesting that it plays a role in the innate immune response, in particular, the acute phase response.
Nam
Bo-Hye
BH
Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research & Development Institute, 408-1 Sirang-ri, Gijang-eup, Gijang-gun, Busan 619-902, Republic of Korea. bhnam@nfrdi.go.kr, bhnam@hanmail.net
Ahn
Kyoung Jin
KJ
Kim
Young-Ok
YO
Kong
Hee Jeong
HJ
Kim
Woo-Jin
WJ
Kim
Hyung Soo
HS
Lee
Sang-Jun
SJ
Kim
Kyong Kil
KK
D000209:Acute-Phase Proteins / Q000737:chemistry / Q000235:genetics*; D000210:Acute-Phase Reaction / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020609:Edwardsiella tarda / Q000276:immunology / Q000472:pathogenicity; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics*; D005432:Flounder / Q000235:genetics* / Q000276:immunology*; D007113:Immunity, Innate / Q000235:genetics; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000276:immunology / Q000472:pathogenicity; D014018:Tissue Distribution; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D029941:Fish Proteins; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein; C021623:bactericidal permeability increasing protein