title: Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos.
abstract: OBJECTIVE
  To report a successful delivery after the transfer of a re-cryopreserved day-7 hatched blastocyst.
  
  
  RESULTS
  A 30-year-old woman underwent a long-treatment protocol for ovarian stimulation. Fourteen mature oocytes were obtained, and twelve were fertilized. On day 3, two cleaved embryos were transferred, but no implantation occurred. The remaining embryos were vitrified. Subsequently, two vitrified day-3 embryos were transferred. The woman became pregnant and delivered a healthy baby. Subsequently, two vitrified day-3 embryos were transferred, but no pregnancy occurred. Subsequently, all the remaining vitrified day-3 embryos were cultured. On day 5, no blastocyst was obtained. The remaining embryos were continued to be cultured. On day 7, a hatched blastocyst was obtained and re-vitrified. Subsequently, the re-vitrified day-7 hatched blastocyst was transferred. The woman became pregnant and delivered a healthy female.
  
  
  CONCLUSIONS
  The day-7 hatched blastocyst developed from vitrified embryos can be re-vitrified and have pregnancy potential after re-warming.
authors.affiliation: Kinutani Women's Clinic, Naka-ku, Hiroshima 730-0035, Japan. hiraoka@chive.ocn.ne.jp
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Hiraoka
authors.forename: Kaori
authors.initials: K
authors.lastname: Hiraoka
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Horiuchi
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Kusuda
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Okano
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kinutani
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kinutani
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology*; D015925:Cryopreservation / Q000379:methods*; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome
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