Nonviral transfection of adipose tissue stromal cells: an experimental study.
Delivery of plasmid DNA and interfering RNA into adipose tissue stromal cells was carried out by the methods of lipofection, calcium phosphate method, and by electroporation. The percent of transfected cells after delivery of plasmid DNA by the calcium phosphate method and lipofection was 0 and 15%, respectively, vs. more than 50% after electroporation. Similar results were obtained for delivery of short-strand RNA into cells. These data indicate that electroporation is the most effective method of nonviral transfection of adipose tissue stromal cells.
Lopatina
T V
TV
Faculty of Basic Medicine, M. V. Lomonosov Moscow State University. t.lopatina@gmail.com
Kalinina
N I
NI
Parfyonova
E V
EV
D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D000071186:Beclin-1; D002130:Calcium Phosphates / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D018274:Electroporation / Q000379:methods*; D051379:Mice; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D051899:Smad2 Protein / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D013997:Time Factors; D014162:Transfection / Q000379:methods*
D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C115010:Becn1 protein, mouse; D002130:Calcium Phosphates; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051899:Smad2 Protein; C497783:Smad2 protein, mouse; D004247:DNA; C020243:calcium phosphate