title: Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis.
abstract: BACKGROUND
  Pathogenic yersiniae, including Y. pestis, share a type III secretion system (T3SS) that is composed of a secretion machinery, a set of translocation proteins, a control system, and six Yop effector proteins including YpkA and YopJ. The cyclic AMP receptor protein (CRP), a global regulator, was recently found to regulate the laterally acquired genes (pla and pst) in Y. pestis. The regulation of T3SS components by CRP is unknown.
  
  
  RESULTS
  The sycO, ypkA and yopJ genes constitute a single operon in Y. pestis. CRP specifically binds to the promoter-proximate region of sycO, and represses the expression of the sycO-ypkA-yopJ operon. A single CRP-dependent promoter is employed for the sycO-ypkA-yopJ operon, but two CRP binding sites (site 1 and site 2) are detected within the promoter region. A CRP box homologue is found in site 1 other than site 2. The determination of CRP-binding sites, transcription start site and core promoter element (-10 and -35 regions) promotes us to depict the structural organization of CRP-dependent promoter, giving a map of CRP-promoter DNA interaction for sycO-ypkA-yopJ.
  
  
  CONCLUSIONS
  The sycO-ypkA-yopJ operon is under the direct and negative regulation of CRP in Y. pestis. The sycO-ypkA-yopJ promoter-proximate regions are extremely conserved in Y. pestis, Y. pseudotuberculosis and Y. enterocolitica. Therefore, data presented here can be generally applied to the above three pathogenic yersiniae.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China. zhanlingjun773@sina.com.cn
authors.forename: Lingjun
authors.initials: L
authors.lastname: Zhan
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Yingli
authors.initials: Y
authors.lastname: Li
authors.forename: He
authors.initials: H
authors.lastname: Gao
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Zhang
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D002373:Cyclic AMP Receptor Protein / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D024363:Transcription Initiation Site; D015010:Yersinia pestis / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D012329:RNA, Bacterial; C109227:YopP protein, Yersinia; C079893:ypkA protein, Yersinia; D017346:Protein Serine-Threonine Kinases
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