title: Identification of genes responsive to solar simulated UV radiation in human monocyte-derived dendritic cells.
abstract: Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC-mediated immune responses.
authors.affiliation: Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Hortensia
authors.initials: H
authors.lastname: de la Fuente
authors.forename: Amalia
authors.initials: A
authors.lastname: Lamana
authors.forename: María
authors.initials: M
authors.lastname: Mittelbrunn
authors.forename: Silvia
authors.initials: S
authors.lastname: Perez-Gala
authors.forename: Salvador
authors.initials: S
authors.lastname: Gonzalez
authors.forename: Amaro
authors.initials: A
authors.lastname: García-Diez
authors.forename: Miguel
authors.initials: M
authors.lastname: Vega
authors.forename: Francisco
authors.initials: F
authors.lastname: Sanchez-Madrid
mesh_terms: D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism / Q000528:radiation effects*; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D009000:Monocytes / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000187:drug effects
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