Quantitative proteomics of seed filling in castor: comparison with soybean and rapeseed reveals differences between photosynthetic and nonphotosynthetic seed metabolism.
Seed maturation or seed filling is a phase of development that plays a major role in the storage reserve composition of a seed. In many plant seeds photosynthesis plays a major role in this process, although oilseeds, such as castor (Ricinus communis), are capable of accumulating oil without the benefit of photophosphorylation to augment energy demands. To characterize seed filling in castor, a systematic quantitative proteomics study was performed. Two-dimensional gel electrophoresis was used to resolve and quantify Cy-dye-labeled proteins expressed at 2, 3, 4, 5, and 6 weeks after flowering in biological triplicate. Expression profiles for 660 protein spot groups were established, and of these, 522 proteins were confidently identified by liquid chromatography-tandem mass spectrometry by mining against the castor genome. Identified proteins were classified according to function, and the most abundant groups of proteins were involved in protein destination and storage (34%), energy (19%), and metabolism (15%). Carbon assimilatory pathways in castor were compared with previous studies of photosynthetic oilseeds, soybean (Glycine max) and rapeseed (Brassica napus). These comparisons revealed differences in abundance and number of protein isoforms at numerous steps in glycolysis. One such difference was the number of enolase isoforms and their sum abundance; castor had approximately six times as many isoforms as soy and rapeseed. Furthermore, Rubisco was 11-fold less prominent in castor compared to rapeseed. These and other differences suggest some aspects of carbon flow, carbon recapture, as well as ATP and NADPH production in castor differs from photosynthetic oilseeds.
Houston
Norma L
NL
Interdisciplinary Plant Group and Department of Biochemistry, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA. houstonn@missouri.edu
Hajduch
Martin
M
Thelen
Jay J
JJ
D029741:Brassica rapa / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002367:Ricinus communis / Q000254:growth & development / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D010788:Photosynthesis / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*
D010940:Plant Proteins; D002244:Carbon
Proteomics. 2001 Mar;1(3):377-96
11680884
Plant Physiol. 2009 May;150(1):27-41
19279196
Anal Chem. 1995 Apr 15;67(8):1426-36
7741214
Annu Rev Plant Biol. 2004;55:69-84
15725057
Arch Biochem Biophys. 1994 Aug 1;312(2):326-35
8037444
Plant Physiol. 2005 Feb;137(2):762-78
15684019
Plant Physiol. 1995 Dec;109(4):1461-1469
12228683
Plant Physiol. 2008 Nov;148(3):1655-67
18820081
Plant Physiol. 1992 Jun;99(2):748-50
16668948
Plant Physiol. 1961 Sep;36(5):540-4
16655549
Plant Physiol. 2008 Mar;146(3):1346-57
18184736
Plant J. 2008 May;54(4):640-55
18476869
J Exp Bot. 2006;57(10):2363-77
16798850
Plant Mol Biol. 2004 Jul;55(5):645-62
15604707
Arch Biochem Biophys. 2004 Sep 15;429(2):134-44
15313216
Plant J. 1998 Apr;14(2):147-57
9628012
J Am Soc Mass Spectrom. 1994 Nov;5(11):976-89
24226387
Plant Physiol. 1991 Dec;97(4):1334-8
16668552
Plant Physiol. 2006 May;141(1):32-46
16543413
Planta. 2005 Dec;222(6):1051-62
16049677
Plant Mol Biol. 1993 Jan;21(2):331-40
8425060
Plant J. 2009 Apr;58(2):220-34
19077167
Plant Physiol. 2005 Apr;137(4):1397-419
15824287
J Mol Evol. 2003 Jul;57(1):16-26
12962302
Eur J Biochem. 2001 May;268(9):2678-86
11322889
Plant Physiol. 1995 Dec;109(4):1327-35
8539293
Plant J. 2007 Oct;52(2):296-308
17683473
Plant Physiol. 2008 Sep;148(1):504-18
18599654
Electrophoresis. 1997 Oct;18(11):2071-7
9420172
Plant J. 1994 Mar;5(3):353-61
8180621
Planta. 2006 Apr;223(5):948-64
16307285
Nature. 1998 Jan 29;391(6666):485-8
9461215
Plant Cell. 2002 Jun;14(6):1191-206
12084821
Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:273-298
15012290
Planta. 1975 Jan;123(2):163-74
24435083
Plant Cell Environ. 2008 Apr;31(4):506-17
18194425
Plant J. 2000 Sep;23(6):723-33
10998184
J Biol Chem. 2003 Aug 8;278(32):29442-53
12759349
J Exp Bot. 2005 May;56(415):1297-303
15767323
Plant Physiol. 1977 Jun;59(6):1128-32
16660007
Plant Physiol. 1992 Apr;98(4):1233-8
16668781
Plant Physiol. 1997 Jul;114(3):851-856
12223747
Biochem J. 1991 Oct 15;279 ( Pt 2):495-501
1953644
Mol Cell Proteomics. 2007 Dec;6(12):2165-79
17848586
Plant J. 2003 Apr;34(1):77-93
12662311
J Biol Chem. 1979 Jul 10;254(13):6094-8
109446
Planta. 2005 Aug;221(6):937-52
15830207
Plant Physiol. 1992 Jun;99(2):445-9
16668905
Plant Physiol. 1979 Jul;64(1):31-7
16660910
Curr Opin Plant Biol. 2003 Jun;6(3):236-46
12753973
Plant Physiol. 2003 Oct;133(2):664-82
12972662
Plant Physiol. 1986 Feb;80(2):301-4
16664617
J Proteome Res. 2007 Aug;6(8):3232-41
17580850
Planta. 2001 Mar;212(4):499-507
11525506
Protein Sci. 1994 Oct;3(10):1883-8
7849603