Analysis of chromosome dynamics and chromosomal proteins in Drosophila spermatocytes.
A wide variety of techniques have been utilized to determine the localization of various proteins from premeiotic through meiotic stages in Drosophila males. Live imaging has been instrumental in monitoring chromosome pairing and the localization of fusion proteins. Immunofluorescence has been a widely utilized technique to examine the localization and colocalization of the many proteins involved in meiosis. Recently, an immuno-FISH protocol was developed to observe the co-localization of DNA probes and proteins. In this chapter, detailed protocols outlining these three types of experiments are presented.
Thomas
Sharon E
SE
Department of Biochemistry, Cellular & Molecular Biology, University of Tennessee, Knoxville, TN, USA.
McKee
Bruce D
BD
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods; D023902:Chromosome Pairing / Q000502:physiology*; D020732:Cytogenetic Analysis / Q000379:methods*; D004330:Drosophila / Q000166:cytology* / Q000235:genetics*; D008297:Male; D008954:Models, Biological; D013090:Spermatocytes / Q000166:cytology* / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone