title: Nicotiana plumbaginifolia plants silenced for the ATP-binding cassette transporter gene NpPDR1 show increased susceptibility to a group of fungal and oomycete pathogens.
abstract: SUMMARY The behaviour of Nicotiana plumbaginifolia plants silenced for the ATP-binding cassette transporter gene NpPDR1 was investigated in response to fungal and oomycete infections. The importance of NpPDR1 in plant defence was demonstrated for two organs in which NpPDR1 is constitutively expressed: the roots and the petal epidermis. The roots of the plantlets of two lines silenced for NpPDR1 expression were clearly more sensitive than those of controls to the fungal pathogens Botrytis cinerea, Fusarium oxysporum sp., F. oxysporum f. sp. nicotianae, F. oxysporum f. sp. melonis and Rhizoctonia solani, as well as to the oomycete pathogen Phytophthora nicotianae race 0. The Ph gene-linked resistance of N. plumbaginifolia to P. nicotianae race 0 was totally ineffective in NpPDR1-silenced lines. In addition, the petals of the NpPDR1-silenced lines were spotted 15%-20% more rapidly by B. cinerea than were the controls. The rapid induction (after 2-4 days) of NpPDR1 expression in N. plumbaginifolia and N. tabacum mature leaves in response to pathogen presence was demonstrated for the first time with fungi and one oomycete: R. solani, F. oxysporum and P. nicotianae. With B. cinerea, such rapid expression was not observed in healthy mature leaves. NpPDR1 expression was not observed during latent infections of B. cinerea in N. plumbaginifolia and N. tabacum, but was induced when conditions facilitated B. cinerea development in leaves, such as leaf ageing or an initial root infection. This work demonstrates the increased sensitivity of NpPDR1-silenced N. plumbaginifolia plants to all of the fungal and oomycete pathogens investigated.
authors.affiliation: Département Biotechnologie, Centre Wallon de Recherches Agronomiques, Chaussée de Charleroi 234, B-5030 Gembloux, Belgium. bultreys@cra.wallonie.be
authors.forename: Alain
authors.initials: A
authors.lastname: Bultreys
authors.forename: Tomasz
authors.initials: T
authors.lastname: Trombik
authors.forename: Anna
authors.initials: A
authors.lastname: Drozak
authors.forename: Marc
authors.initials: M
authors.lastname: Boutry
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D020171:Botrytis / Q000502:physiology*; D035264:Flowers / Q000235:genetics / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D005966:Glucuronidase / Q000378:metabolism; D009868:Oomycetes / Q000502:physiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology; D014026:Nicotiana / Q000235:genetics* / Q000382:microbiology*
chemical_list: D018528:ATP-Binding Cassette Transporters; D010940:Plant Proteins; D005966:Glucuronidase
references.citation: J Biol Chem. 1998 Dec 25;273(52):34837-42
references.pmid: 9857010
references.citation: Plant Mol Biol. 2004 Apr;54(6):817-35
references.pmid: 15604654
references.citation: Plant Cell. 2000 Nov;12(11):2191-200
references.pmid: 11090218
references.citation: Plant Dis. 1997 Jun;81(6):604-606
references.pmid: 30861843
references.citation: DNA Res. 2006 Oct 31;13(5):205-28
references.pmid: 17164256
references.citation: Plant Physiol. 2005 Jun;138(2):827-36
references.pmid: 15923333
references.citation: Plant Dis. 1997 Nov;81(11):1269-1274
references.pmid: 30861732
references.citation: Phytopathology. 2003 Mar;93(3):316-22
references.pmid: 18944341
references.citation: Plant J. 2003 Jul;35(2):237-50
references.pmid: 12848828
references.citation: Plant Dis. 1999 Aug;83(8):777-780
references.pmid: 30845568
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Plant Cell. 2001 May;13(5):1095-107
references.pmid: 11340184
references.citation: J Exp Bot. 2007;58(7):1617-26
references.pmid: 17426057
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: J Mol Biol. 1999 Oct 22;293(2):381-99
references.pmid: 10529352
references.citation: FEBS Lett. 2002 May 8;518(1-3):164-8
references.pmid: 11997039
references.citation: FEBS Lett. 2006 Feb 13;580(4):1123-30
references.pmid: 16506311
references.citation: Curr Opin Struct Biol. 2004 Aug;14(4):426-31
references.pmid: 15313236
references.citation: Plant Dis. 2005 Nov;89(11):1220-1228
references.pmid: 30786447
references.citation: Plant Physiol. 2005 Sep;139(1):341-52
references.pmid: 16126865
references.citation: Annu Rev Plant Biol. 2007;58:347-75
references.pmid: 17263663
references.citation: Genetics. 2008 Dec;180(4):2237-50
references.pmid: 18845849
references.citation: Nat New Biol. 1973 Jul 4;244(131):29-30
references.pmid: 4515911
references.citation: Trends Plant Sci. 2008 Apr;13(4):151-9
references.pmid: 18299247
references.citation: Mycologia. 2002 May-Jun;94(3):437-49
references.pmid: 21156515
references.citation: Plant Dis. 2005 Mar;89(3):332-337
references.pmid: 30795358
references.citation: Phytopathology. 1999 Mar;89(3):197-203
references.pmid: 18944759
references.citation: Plant Cell Physiol. 2006 Mar;47(3):309-18
references.pmid: 16415066
references.citation: Plant Dis. 2005 Dec;89(12):1285-1294
references.pmid: 30791306
references.citation: Plant Dis. 2002 Oct;86(10):1080-1084
references.pmid: 30818499
references.citation: Plant Dis. 1998 May;82(5):530-536
references.pmid: 30856983
references.citation: J Exp Bot. 2006;57(10):2193-201
references.pmid: 16720608
references.citation: Plant Cell. 2006 Mar;18(3):731-46
references.pmid: 16473969
references.citation: Plant J. 2002 Jun;30(6):649-62
references.pmid: 12061897
references.citation: Plant Physiol. 2003 Nov;133(3):1272-84
references.pmid: 14526118
references.citation: Plant Dis. 2003 Apr;87(4):351-358
references.pmid: 30831828
references.citation: Plant Physiol. 2003 Mar;131(3):1169-77
references.pmid: 12644668
references.citation: Phytochemistry. 2001 Jul;57(5):689-92
references.pmid: 11397435
references.citation: Planta. 2002 Nov;216(1):95-106
references.pmid: 12430018