title: Inhibitory effect of essential oils of Allium sativum and Piper longum on spontaneous muscular activity of liver fluke, Fasciola gigantica.
abstract: Effects of essential oil of Allium sativum (garlic) and Piper longum (Indian long pepper) were evaluated on muscular activity of whole Fasciola gigantica and its strip preparation. The whole flukes and longitudinal strip preparations of the flukes were isometrically mounted to record the spontaneous muscular activity (SMA) and to evaluate effects of cumulative doses (0.1, 0.3, 1.0 and 3.0mg/ml) of the plant essential oils. Whole flukes and the strip preparations exhibited continuous SMA without any significant difference in its baseline tension, frequency and amplitude for 2h. Essential oil of A. sativum produced significant reduction in the frequency and the amplitude of the SMA of whole fluke at 1 and 3mg/ml concentrations. It caused complete paralysis of the fluke after 15 min of administration of 3mg/ml concentration. Similar to whole fluke, essential oil of A. sativum (3mg/ml) also produced flaccid paralysis in the strip preparations of the flukes. Essential oil of P. longum firstly induced marked excitatory effect and then there was flaccid paralysis of the whole fluke following 15 min exposure at 3mg/ml concentration. Complete flaccid paralysis of the strip preparation was also ensued after 15 min of administration of 3mg/ml concentration of P. longum. In both the essential oils, the whole fluke and strip preparations did not recover from paralysis following 2-3 washes. In conclusion, the observations demonstrated irreversible paralytic effect of essential oils of A. sativum and P. longum on F. giganticain vitro which might possibly help to developing herbal-based anthelmintic.
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243122 (U.P), India. tusingh80@gmail.com
authors.forename: Thakur Uttam
authors.initials: TU
authors.lastname: Singh
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.forename: Surendra Kumar
authors.initials: SK
authors.lastname: Tandan
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Mishra
mesh_terms: D000818:Animals; D002020:Buffaloes; D005209:Fasciola / Q000187:drug effects* / Q000502:physiology; D005737:Garlic / Q000737:chemistry*; D009068:Movement / Q000187:drug effects; D009132:Muscles / Q000187:drug effects / Q000502:physiology; D009822:Oils, Volatile / Q000494:pharmacology*; D031701:Piper / Q000737:chemistry*; D010938:Plant Oils / Q000494:pharmacology*
chemical_list: D009822:Oils, Volatile; D010938:Plant Oils