Imaging vasculature and lymphatic flow in mice using quantum dots.
Quantum dots are ideal probes for fluorescent imaging of vascular and lymphatic tissues. On injection into appropriate sites, red- and near-infrared-emitting quantum dots provide excellent definition of vasculature, lymphoid organs, and lymph nodes draining both normal tissues and tumors. We detail methods for use with commercially available quantum dots and discuss common difficulties.
Ballou
Byron
B
Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, PA, USA.
Ernst
Lauren A
LA
Andreko
Susan
S
Fitzpatrick
James A J
JA
Lagerholm
B Christoffer
BC
Waggoner
Alan S
AS
Bruchez
Marcel P
MP
D000818:Animals; D001808:Blood Vessels / Q000503:physiopathology*; D005453:Fluorescence; D042601:Lymphatic Vessels / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology; D045663:Quantum Dots; D021701:Sentinel Lymph Node Biopsy