Effect of histological processing and methacrylate sectioning on the area of gill tissue in teleost.
Deformation of biological tissues may occur during histological processing and results in loss of accuracy when quantitative information about cells, tissues and organs is necessary. In this study, the gill tissue from armored catfish (Pterygoplichthys anisitsi) was quantified in each step of processing using the stereological principles. During processing for glycol methacrylate embedding, gill tissue from shrinks significantly but regains its original dimensions after sectioning.
Cruz
A L
AL
Departamento de Ciências Fisiológicas, Universidade Federal de São Carlos, São Carlos, SP, Brazil, 13565-905.
Fernandes
M N
MN
Perry
S F
SF
D000818:Animals; D002397:Catfishes / Q000033:anatomy & histology*; D005880:Gills / Q000033:anatomy & histology*; D008689:Methacrylates / Q000737:chemistry*; D016610:Tissue Embedding / Q000379:methods*
D008689:Methacrylates; C005044:hydroxyethyl methacrylate