title: Flow injection-capillary electrophoresis frontal analysis method for the study of the interactions of a series of drugs with human serum albumin.
abstract: In this paper, a fast method for the study on the interactions of a series of drugs used in the treatment of hypertension with human serum albumin (HSA) by flow injection-capillary electrophoresis (FI-CE) was developed based on the principle of frontal analysis (FA). The binding parameters were determined by FI-CE-FA from Scatchard equation and compared with results obtained by non-CE methods and literature values. A multiple linear regression (MLR) model between the drug-protein binding constants (K) and structural descriptors of drugs was constructed. L-tryptophan (L-try) and phenylbutazone (PB) were used as displacement reagents to investigate the binding sites of a series of drugs on HSA. The binding synergism effect between drugs and the effects of many metal ions existing in human plasma on protein binding were also investigated systematically.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Liu
authors.forename: Shuyan
authors.initials: S
authors.lastname: Li
authors.forename: Jingshu
authors.initials: J
authors.lastname: Zhang
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D004357:Drug Synergism; D019075:Electrophoresis, Capillary / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008670:Metals / Q000737:chemistry / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000378:metabolism; D021281:Quantitative Structure-Activity Relationship; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008670:Metals; D004364:Pharmaceutical Preparations; D012709:Serum Albumin