title: Long-term responses of canine lungs to acidic particles.
abstract: Sixteen beagle dogs were housed in four large chambers under minimum restraint. They were exposed for 16 months to clean air and individual baseline data of markers were obtained. For 13 months, eight dogs were further exposed to clean air and eight dogs for 6 h/d to 1-microm MMAD (mass median aerodynamic diameter) acidic sulfate particles carrying 25 micromol H(+) m(-3) into their lungs. To establish functional responses (lung function, cell and tissue integrity, redox balance, and non-specific respiratory defense capacity), each exposed animal served as its own control. To establish structural responses, the eight non-exposed animals served as controls. Acidic particles were produced by nebulization of aqueous sodium hydrogen sulfate at pH 1.5. Only subtle exposure-related changes of lung function and structure were detected. A significant increase in respiratory burst function of alveolar macrophages points to a marginal inflammatory response. This can be explained by the significant production of prostaglandin E(2), activating cyclooxygenase-dependent mechanisms in epithelia and thus inhibiting lung inflammation. The non-specific defense capacity was slightly affected, giving increased tracheal mucus velocity and reduced in vivo dissolution of moderately soluble test particles. Hypertrophy and hyperplasia of bronchial epithelia were not observed, but there was an increase in volume density of bronchial glands and a shift from neutral to acidic staining of epithelial secretory cells in distal airways. The acidic exposure had thus no pathophysiological consequences. It is therefore unlikely that long-term inhalation of acidic particles is associated with a health risk.
authors.affiliation: Institute for Inhalation Biology, Helmholtz Zentrum München-German Research Center for Environmental Health, Neuherberg/Munich, Germany. joachim.heyder@helmholtz-muenchen.de
authors.forename: J
authors.initials: J
authors.lastname: Heyder
authors.forename: I
authors.initials: I
authors.lastname: Beck-Speier
authors.forename: G A
authors.initials: GA
authors.lastname: Ferron
authors.forename: M
authors.initials: M
authors.lastname: Josten
authors.forename: E
authors.initials: E
authors.lastname: Karg
authors.forename: W G
authors.initials: WG
authors.lastname: Kreyling
authors.forename: A-G
authors.initials: AG
authors.lastname: Lenz
authors.forename: K L
authors.initials: KL
authors.lastname: Maier
authors.forename: P
authors.initials: P
authors.lastname: Reitmeier
authors.forename: L
authors.initials: L
authors.lastname: Ruprecht
authors.forename: S
authors.initials: S
authors.lastname: Takenaka
authors.forename: T
authors.initials: T
authors.lastname: Wohland
authors.forename: A
authors.initials: A
authors.lastname: Ziesenis
authors.forename: H
authors.initials: H
authors.lastname: Schulz
mesh_terms: D000143:Acids / Q000633:toxicity*; D000336:Aerosols; D000818:Animals; D001273:Atmosphere Exposure Chambers; D004285:Dogs; D019570:Inhalation Exposure; D008168:Lung / Q000473:pathology*; D008171:Lung Diseases / Q000139:chemically induced / Q000473:pathology; D008297:Male; D010084:Oxidation-Reduction; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D012129:Respiratory Function Tests; D013431:Sulfates / Q000737:chemistry / Q000633:toxicity
chemical_list: D000143:Acids; D000336:Aerosols; D052638:Particulate Matter; D013431:Sulfates