title: Rapid production of protein-loaded biodegradable microparticles using surface acoustic waves.
abstract: We present a straightforward and rapid surface acoustic wave (SAW) atomization-based technique for encapsulating proteins into 10 mum order particles composed of a biodegradable polymeric excipient, using bovine serum albumin (BSA) as an exemplar. Scans obtained from confocal microscopy provide qualitative proof of encapsulation and show the fluorescent conjugated protein to be distributed in a relatively uniform manner within the polymer shell. An ELISA assay of the collected particles demonstrates that the BSA survives the atomization, particle formation, and collection process with a yield of approximately 55%. The SAW atomization universally gave particles with a textured morphology, and increasing the frequency and polymer concentration generally gave smaller particles (to 3 mum average) with reduced porosity.
authors.forename: Mar
authors.initials: M
authors.lastname: Alvarez
authors.forename: Leslie Y
authors.initials: LY
authors.lastname: Yeo
authors.forename: James R
authors.initials: JR
authors.lastname: Friend
authors.forename: Milan
authors.initials: M
authors.lastname: Jamriska
references.citation: Langmuir. 2008 Oct 7;24(19):10629-32
references.pmid: 18795809
references.citation: Int J Pharm. 2004 Oct 13;284(1-2):13-21
references.pmid: 15454292
references.citation: Adv Drug Deliv Rev. 2005 Jan 10;57(3):357-76
references.pmid: 15560946
references.citation: Biomicrofluidics. 2009 Jan 02;3(1):12002
references.pmid: 19693383
references.citation: Pharm Res. 2000 Nov;17(11):1374-83
references.pmid: 11205730
references.citation: J Colloid Interface Sci. 2007 Jun 15;310(2):529-35
references.pmid: 17346734
references.citation: Science. 2002 Mar 1;295(5560):1695-8
references.pmid: 11872835
references.citation: Int J Pharm. 1999 Jul 5;184(1):73-84
references.pmid: 10425353
references.citation: J Control Release. 2000 Dec 3;69(3):435-44
references.pmid: 11102683
references.citation: Anal Bioanal Chem. 2004 Aug;379(7-8):982-91
references.pmid: 15257426
references.citation: Phys Rev Lett. 2004 Apr 2;92(13):133902
references.pmid: 15089614
references.citation: J Mater Sci Mater Med. 2008 Apr;19(4):1703-11
references.pmid: 17914622
references.citation: J Control Release. 2004 Mar 5;95(2):185-95
references.pmid: 14980767
references.citation: Phys Rev Lett. 2008 Aug 22;101(8):084502
references.pmid: 18764621
references.citation: Biomaterials. 2007 Oct;28(28):4098-104
references.pmid: 17588654
references.citation: Nanotechnology. 2008 Apr 9;19(14):145301
references.pmid: 21817755
references.citation: Biomaterials. 2004 Aug;25(19):4741-8
references.pmid: 15120520
references.citation: Biomed Microdevices. 2007 Oct;9(5):647-56
references.pmid: 17530412
references.citation: Biomaterials. 2005 Nov;26(31):6122-8
references.pmid: 15893816