Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice.
Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for DNA and genome engineering. Cre has long been recognized as the best SSR for genome engineering, particularly in mice. Obtaining another SSR that is as good as Cre will be a valuable addition to the genomic toolbox. To this end, we have developed and validated reagents for the Dre-rox system. These include an Escherichia coli-inducible expression vector based on the temperature-sensitive pSC101 plasmid, a mammalian expression vector based on the CAGGs promoter, a rox-lacZ reporter embryonic stem (ES) cell line based on targeting at the Rosa26 locus, the accompanying Rosa26-rox reporter mouse line, and a CAGGs-Dre deleter mouse line. We also show that a Dre-progesterone receptor shows good ligand-responsive induction properties. Furthermore, we show that there is no crossover recombination between Cre-rox or Dre-loxP. Hence, we add another set of efficient tools to the genomic toolbox, which will enable the development of more sophisticated mouse models for the analysis of gene function and disease.
Anastassiadis
Konstantinos
K
Center for Regenerative Therapies Dresden, BioInnovationsZentrum Technische Universitaet Dresden, Am Tatzberg 47, 01307 Dresden, Germany.
Fu
Jun
J
Patsch
Christoph
C
Hu
Shengbiao
S
Weidlich
Stefanie
S
Duerschke
Kristin
K
Buchholz
Frank
F
Edenhofer
Frank
F
Stewart
A Francis
AF
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D018390:Gene Targeting; D017930:Genes, Reporter; D019426:Integrases / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D015735:Mifepristone / Q000494:pharmacology; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011374:Progesterone / Q000378:metabolism; D011387:Prokaryotic Cells / Q000187:drug effects / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D045522:Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics; D015203:Reproducibility of Results
C546029:Dre recombinase, E coli; D029968:Escherichia coli Proteins; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D008024:Ligands; D011506:Proteins; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins; D045522:Recombinases; D015735:Mifepristone; D011374:Progesterone; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C077570:Site-specific recombinase