title: Synthesis and characterization of a redox-active ion channel supporting cation flux in lipid bilayers.
abstract: The synthesis, cation binding and transmembrane conductive properties of a novel synthetic ion channel containing a redox-active ferrocene unit are described. Fluorescence spectroscopy was used to demonstrate that the channel supports multiple ion coordination and association constants for 1:1 and 1:2 (channel:cation) coordination for both Na(+) and K(+) were evaluated. Experiments using a black lipid membrane preparation revealed that this compound functioned effectively as an ion channel for both Na(+) and K(+). Concomitant (23)Na NMR spectroscopy studies supported this finding and revealed a Na(+) flux, at least 5 times higher than ion transport rates by monensin. Furthermore, oxidation of the redox-active centre (Fe(2+) to Fe(3+)) effectively inhibited ion transport.
authors.affiliation: Center for Heterocyclic Compounds, Department of Chemistry, 126 Sisler Hall, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Maia
authors.initials: M
authors.lastname: Tsikolia
authors.forename: Adam C
authors.initials: AC
authors.lastname: Hall
authors.forename: Cristina
authors.initials: C
authors.lastname: Suarez
authors.forename: Zazi O
authors.initials: ZO
authors.lastname: Nylander
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Wardlaw
authors.forename: Molly E
authors.initials: ME
authors.lastname: Gibson
authors.forename: Kerry L
authors.initials: KL
authors.lastname: Valentine
authors.forename: Louisa N
authors.initials: LN
authors.lastname: Onyewadume
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Ahove
authors.forename: Maya
authors.initials: M
authors.lastname: Woodbury
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Mongare
authors.forename: C Dennis
authors.initials: CD
authors.lastname: Hall
authors.forename: Zuoquan
authors.initials: Z
authors.lastname: Wang
authors.forename: Bogdan
authors.initials: B
authors.lastname: Draghici
authors.forename: Alan R
authors.initials: AR
authors.lastname: Katritzky
mesh_terms: D001692:Biological Transport; D002412:Cations / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004553:Electric Conductivity; D005296:Ferrous Compounds / Q000737:chemistry; D007473:Ion Channels / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D000075163:Metallocenes; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence
chemical_list: D002412:Cations; D005296:Ferrous Compounds; D007473:Ion Channels; D008051:Lipid Bilayers; D000075163:Metallocenes; C004998:ferrocene