[Molecular identification on Waxy genes in wheat using multiple-PCR].
Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected simultaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality properties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat.
Lu
Long-Dou
LD
College of Life Science, Henan Normal University, Xinxiang 453007, China. lld5910@yahoo.com
Hou
Cai-Ling
CL
Chen
Long
L
Yin
Gui-Hong
GH
Deng
Chuan-Liang
CL
Gao
Wu-Jun
WJ
Yang
Xu-Qin
XQ
Tan
Guang-Xuan
GX
D001947:Breeding; D017931:DNA Primers / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D013214:Starch Synthase / Q000235:genetics* / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D010940:Plant Proteins; C060756:waxy protein, plant; D013214:Starch Synthase