Bioavailability and metabolism of fucoxanthin in rats: structural characterization of metabolites by LC-MS (APCI).
This study reports bioavailability and metabolism of fucoxanthin (FUCO) from brown algae Padina tetrastromatica in rats. Rats were divided into two groups (n = 25/group). Group one was fed basal diet (control) while the group two received retinol deficient diet (RD group) for 8 weeks. After confirmed RD in blood (0.53 micromol/l), rats were further sub-grouped (n = 5/sub group), intubated a dose of FUCO (0.83 micromol) and killed after 0, 2, 4, 6 and 8 h. The plasma levels (area under curve/8 h) of FUCO (fucoxanthinol (FUOH) + amarouciaxanthin (AAx)) was 2.93 (RD group) and 2.74 pmol/dl (control), respectively. No newly formed retinol was detected in RD rats intubated with FUCO. Besides FUOH (m/z 617 (M+H)(+)) and AAx (m/z 617 (M+H(-))(+)), other deacetylated, hydrolyzed and demethylated metabolites of bearing molecular mass at m/z 600.6 (FUOH-H(2)O), m/z 597 (AAx-H(2)O), m/z 579 (AAx-2H(2)O+1), m/z 551 (AAx-2H(2)O-2CH(3)+2) and m/z 523 (AAx-2H(2)O-4CH(3)+4) were also detected in plasma and liver by LC-MS (APCI). Although biological functions of FUCO metabolites need thorough investigation, this is the first detailed report on FUCO metabolites in rats.
Sangeetha
Ravi Kumar
RK
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, CSIR, Mysore, 570 020, India.
Bhaskar
Narayan
N
Divakar
Sounder
S
Baskaran
Vallikannan
V
D000818:Animals; D001682:Biological Availability; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D008660:Metabolism; D055432:Metabolomics / Q000379:methods; D015394:Molecular Structure; D000459:Phaeophyceae / Q000737:chemistry; D051381:Rats; D053719:Tandem Mass Spectrometry; D024341:Xanthophylls / Q000378:metabolism / Q000493:pharmacokinetics*
D024341:Xanthophylls; C025164:fucoxanthin
Mol Cell Biochem. 1997 Oct;175(1-2):43-8
9350032
Arch Biochem Biophys. 2004 Oct 1;430(1):110-9
15325918
Mol Cell Biochem. 2006 Aug;288(1-2):7-15
16845492
Drug Metab Dispos. 2004 Feb;32(2):205-11
14744942
J Nutr. 2001 Dec;131(12):3303-6
11739884
J Nutr. 2006 Jun;136(6):1552-7
16702320
J Nutr. 2002 May;132(5):946-51
11983819
Biochem Biophys Res Commun. 2005 Jul 1;332(2):392-7
15896707
Free Radic Biol Med. 2008 Oct 1;45(7):982-93
18640265
J Nutr. 2004 Jan;134(1):237S-240S
14704326
Mol Cell Biochem. 2009 Nov;331(1-2):59-67
19421712
Eur J Nutr. 2008 Dec;47(8):432-41
18853231
Exp Eye Res. 2005 Oct;81(4):422-8
15950219
J Nutr. 1977 Jul;107(7):1340-8
874577
World Rev Nutr Diet. 1985;45:199-220
3887776
J Agric Food Chem. 2007 Oct 17;55(21):8516-22
17894451
Indian J Biochem Biophys. 1981 Feb;18(1):88
7275161
Invest Ophthalmol Vis Sci. 2007 Apr;48(4):1435-40
17389468
Invest Ophthalmol Vis Sci. 2002 Nov;43(11):3383-92
12407147
Comp Biochem Physiol A Mol Integr Physiol. 1998 Apr;119(4):963-74
9773489