The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA.
There are three stages of transcribing DNA into RNA. These stages are initiation, elongation and termination, and they are well-understood biochemically. However, despite the plethora of structural information made available on RNA polymerase in the last decade, little is available for RNA polymerase in complex with transcription elongation factors. To understand the mechanisms of transcriptional regulation, we describe the first structure, to our knowledge, for a bacterial RNA polymerase in complex with an essential transcription elongation factor. The resulting structure formed between the RNA polymerase and NusA from Bacillus subtilis provides important insights into the transition from an initiation complex to an elongation complex, and how NusA is able to modulate transcription elongation and termination.
Yang
Xiao
X
School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia.
Molimau
Seeseei
S
Doherty
Geoff P
GP
Johnston
Elecia B
EB
Marles-Wright
Jon
J
Rothnagel
Rosalba
R
Hankamer
Ben
B
Lewis
Richard J
RJ
Lewis
Peter J
PJ
D001412:Bacillus subtilis / Q000737:chemistry* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008958:Models, Molecular; D010445:Peptide Elongation Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D010445:Peptide Elongation Factors; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit; C105078:RNA polymerase beta subunit
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