title: Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).
abstract: Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China.
authors.forename: Feng-Shan
authors.initials: FS
authors.lastname: Gao
authors.forename: Gui-Xue
authors.initials: GX
authors.lastname: Hu
authors.forename: Xian-zhu
authors.initials: XZ
authors.lastname: Xia
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Gao
authors.forename: Ya-Duo
authors.initials: YD
authors.lastname: Bai
authors.forename: Xiao-Huan
authors.initials: XH
authors.lastname: Zou
mesh_terms: D000595:Amino Acid Sequence; D000820:Animal Diseases / Q000821:virology*; D000818:Animals; D000836:Animals, Zoo / Q000821:virology*; D003333:Coronaviridae Infections / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000235:genetics / Q000302:isolation & purification*; D017809:Fatal Outcome; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D001503:Ursidae / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D014764:Viral Proteins
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