Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidicnanofluidic devices.
This paper presents a study of electrokinetic transport in single nanopores integrated into vertically stacked three-dimensional hybrid microfluidicnanofluidic structures. In these devices, single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e., DNA) polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electro-osmotic transport is predominant over electrophoresis in single nanopores with d>180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.
King
Travis L
TL
Gatimu
Enid N
EN
Bohn
Paul W
PW
Phys Rev E Stat Nonlin Soft Matter Phys. 2006 Oct;74(4 Pt 1):041901
17155090
Analyst. 2003 Jun;128(6):537-42
12866863
J Phys Chem B. 2008 Nov 27;112(47):14687-91
18973375
Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9127-32
15961544
Biophys J. 2008 Jul;95(1):273-86
18339746
Lab Chip. 2006 May;6(5):667-74
16652183
Sens Actuators B Chem. 2008 Jun 16;132(2):593-600
19584949
Langmuir. 2007 Dec 18;23(26):13209-22
17999544
Anal Chem. 2006 Apr 1;78(7):2335-41
16579617
Nano Lett. 2008 Jun;8(6):1683-8
18459741
Lab Chip. 2008 Oct;8(10):1625-31
18813383
Phys Rev Lett. 2007 Jun 8;98(23):238104
17677940
J Am Chem Soc. 2005 Oct 12;127(40):13928-33
16201814
J Am Chem Soc. 2007 Jun 20;129(24):7620-6
17530757
Angew Chem Int Ed Engl. 2004 Mar 26;43(14):1862-5
15054797
Anal Chem. 2004 Nov 1;76(21):6419-25
15516136
Rev Sci Instrum. 2008 Jun;79(6):063702
18601408
Phys Rev Lett. 2008 Apr 18;100(15):158302
18518160
J Phys Chem B. 2007 Jul 26;111(29):8477-85
17604391
Biophys J. 2006 Jun 15;90(12):4538-45
16732056
J Chem Phys. 2007 Apr 21;126(15):154904
17461664