Identification of Phytophthora sojae genes involved in asexual sporogenesis.
To explore the molecular mechanisms involved in asexual spore development in Phytophthora sojae, the zoospores of strain PS26 were treated with ultraviolet (UV) irradiation. After selection, a mutant progeny, termed PS26-U03, was obtained and demonstrated to exhibit no oospore production. A suppression subtractive hybridization (SSH) approach was developed to investigate differences in gene expression between PS26 and PS26-U03 during asexual sporogenesis. Of the 126 sequences chosen for examination, 39 putative unigenes were identified that exhibit high expression in PS26. These sequences are predicted to encode proteins involved in metabolism, cell cycle, protein biosynthesis, cell signalling, cell defence, and transcription regulation. Seven clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. Three of the selected genes, developmental protein DG1037 (UB88), glycoside hydrolase (UB149) and a hypothetical protein (UB145), were expressed only in PS26, whereas the transcripts of phosphatidylinositol-4-phosphate 5-kinase (UB36), FAD-dependent pyridine nucleotide-disulphide oxidoreductase (UB226) and sugar transporter (UB256) were expressed at very low levels in PS26-U03 but at high levels in PS26.
Wang
Ziying
Z
Department of Life Science, Hefei Teachers College, Hefei, Anhui 230061, People's Republic of China. sjywzy@yahoo.com.cn
Wang
Zhaoxia
Z
Shen
Jie
J
Wang
Guangyue
G
Zhu
Xiaoxi
X
Lu
Hongxia
H
D003001:Cloning, Molecular; D018076:DNA, Complementary; D005800:Genes, Fungal; D009693:Nucleic Acid Hybridization; D010838:Phytophthora / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013172:Spores, Fungal; D013382:Subtraction Technique
D018076:DNA, Complementary
Annu Rev Physiol. 2001;63:289-325
11181958
Fungal Genet Biol. 2003 Feb;38(1):54-62
12553936
Science. 1993 Feb 12;259(5097):946-51
8438152
Mol Plant Microbe Interact. 1997 Dec;10(9):1045-53
9390419
Fungal Genet Biol. 1999 Nov;28(2):94-106
10587472
Mol Microbiol. 2002 Aug;45(4):1057-66
12180924
Fungal Genet Biol. 2004 Feb;41(2):115-28
14732258
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
9843981
J Biol Chem. 2000 Jan 14;275(2):1495-501
10625703
J Cell Sci. 2002 Dec 1;115(Pt 23):4565-75
12415001
Mol Plant Microbe Interact. 2005 Mar;18(3):229-43
15782637
Nucleic Acids Res. 2002 Jan 1;30(1):31-4
11752246
EMBO Rep. 2001 Nov;2(11):1013-7
11713193
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6025-30
8650213
Mol Plant Pathol. 2007 Jan;8(1):1-8
20507474
Mol Plant Microbe Interact. 2002 Feb;15(2):102-8
11876422
Science. 2006 Sep 1;313(5791):1261-6
16946064
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:281-309
15012236
Fungal Genet Biol. 2003 Mar;38(2):187-97
12620255
Fungal Genet Biol. 2001 Dec;34(3):217-27
11728159
Nature. 2002 Jul 11;418(6894):244-51
12110901
Nature. 1998 Feb 19;391(6669):806-11
9486653
FEMS Microbiol Lett. 2006 Nov;264(2):198-204
17064373
Curr Genet. 2005 May;47(5):307-15
15815927
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694