Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells.
A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency in comparison to heat shock method and is the first reported chemically-induced transformation. New data about interactions between cholates and biomembranes is revealed that may contribute to better understanding of bacterial transformation.
Ravnikar
Matjaz
M
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000, Ljubljana, Slovenia. matjaz.ravnikar@ffa.uni-lj.si
Irman
Andreja
A
Radić
Natasa
N
Lunder
Mojca
M
Strukelj
Borut
B
D002463:Cell Membrane Permeability / Q000187:drug effects*; D020355:Cholates / Q000378:metabolism*; D012503:Saponins / Q000378:metabolism*; D014169:Transformation, Bacterial
D020355:Cholates; D012503:Saponins