Alamethicin topology in phospholipid membranes by oriented solid-state NMR and EPR spectroscopies: a comparison.
Alamethicin, a hydrophobic peptide that is considered a paradigm for membrane channel formation, was uniformly labeled with 15N, reconstituted into oriented phosphatidylcholine bilayers at concentrations of 1 or 5 mol %, and investigated by solid-state NMR spectroscopy as a function of temperature. Whereas the peptide adopts a transmembrane alignment in POPC bilayers at all temperatures investigated, it switches from a transmembrane to an in-plane orientation in DPPC membranes when passing the phase transition temperature. This behavior can be explained by an increase in membrane hydrophobic thickness and the resulting hydrophobic mismatch condition. Having established the membrane topology of alamethicin at temperatures above and below the phase transition, ESEEM EPR was used to investigate the water accessibility of alamethicin synthetic analogues carrying the electron spin label TOAC residue at one of positions 1, 8, or 16. Whereas in the transmembrane alignment the labels at positions 8 and 16 are screened from the water phase, this is only the case for the latter position when adopting an orientation parallel to the surface. By comparing the EPR and solid-state NMR data of membrane-associated alamethicin it becomes obvious that the TOAC spin labels and the cryo-temperatures required for EPR spectroscopy have less of an effect on the alamethicin-POPC interactions when compared to DPPC. Finally, at P/L ratios of 1/100, spectral line broadening due to spin-spin interactions and thereby peptide oligomerization within the membrane were detected for transmembrane alamethicin.
Salnikov
Evgeniy S
ES
Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, 630090 Novosibirsk, Russian Federation.
De Zotti
Marta
M
Formaggio
Fernando
F
Li
Xing
X
Toniolo
Claudio
C
O'Neil
Joe D J
JD
Raap
Jan
J
Dzuba
Sergei A
SA
Bechinger
Burkhard
B
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000408:Alamethicin / Q000737:chemistry*; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D004583:Electrons; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013113:Spin Labels; D013499:Surface Properties; D014242:Trichoderma / Q000378:metabolism
D008055:Lipids; D010455:Peptides; D010713:Phosphatidylcholines; D010743:Phospholipids; D013113:Spin Labels; D015060:1,2-Dipalmitoylphosphatidylcholine; D000408:Alamethicin; C028694:1-palmitoyl-2-oleoylphosphatidylcholine