In vivo myocardial distribution of multipotent progenitor cells following intracoronary delivery in a swine model of myocardial infarction.
OBJECTIVE
There are few data comparing the fate of multipotent progenitor cells (MPCs) used in cardiac cell therapy after myocardial infarction (MI). To document in vivo distribution of MPCs delivered by intracoronary (IC) injection.


RESULTS
Using an anterior MI swine model, near-infrared (NIR) fluorescence was used for in vivo tracking of labelled MPCs [mesenchymal stromal (MSCs), bone marrow mononuclear (BMMNCs), and peripheral blood mononuclear (PBMNCs)] cells early after IC injection. Signal intensity ratios (SIRs) of injected over non-injected (reference) zones were used to report NIR fluorescence emission. Following IC injection, significant differences in mean SIR were documented when MSCs were compared with BMMNCs [1.28 +/- 0.10 vs. 0.77 +/- 0.11, P < 0.001; 95% CI (0.219, 0.805), respectively] or PBMNCs [1.28 +/- 0.10 vs. 0.80 +/- 0.14, P = 0.005; 95% CI (0.148, 0.813), respectively]. Differences were maintained during the 60 min tracking period, with only the MSC-injected groups continuously emitting NIR fluorescence (SIR>1). This is correlated with greater cell retention for MSCs relative to mononuclear cells. However, there was evidence of MSC-related vessel plugging in some swine.


CONCLUSIONS
Our in vivo NIR fluorescence findings suggest that MPC distribution and retention immediately after intracoronary delivery vary depending on cell population and could potentially impact the clinical efficacy of cardiac cell therapy.
Ly
Hung Q
HQ
Mount Sinai School of Medicine, New York, NY, USA. hung.ly@icm-mhi.org
Hoshino
Kozo
K
Pomerantseva
Irina
I
Kawase
Yoshiaki
Y
Yoneyama
Ryuichi
R
Takewa
Yoshiaki
Y
Fortier
Annik
A
Gibbs-Strauss
Summer L
SL
Vooght
Carrie
C
Frangioni
John V
JV
Hajjar
Roger J
RJ
D000818:Animals; D002470:Cell Survival; D003326:Coronary Circulation / Q000502:physiology; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D007270:Injections, Intra-Articular; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000637:transplantation; D009203:Myocardial Infarction / Q000628:therapy*; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D033581:Stem Cell Transplantation / Q000379:methods*; D013552:Swine
D005456:Fluorescent Dyes
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