title: The glutathione-S-transferase gene polymorphisms (Gstt1, Gstm1, and Gstp1) in patients with non-allergic nasal polyposis.
abstract: There is an ongoing dilemma about the pathogenesis of nasal polyp (NP). The etiology of NP is multifactorial. Reactive oxygen species and oxidative stress are also suggested to be among the possible factors in NP development. Glutathione-S-transferase (GST) is one of the important detoxifying enzymes. It is not known whether GST plays any role in NP development. We aimed to investigate the relationship between GST subgroup (GSTT1, GSTM1, and GSTP1) polymorphisms, and NP development. Seventy-five patients with NP with or without asthma (NP with asthma: 22, NP without asthma: 53) were used as a study group. As much as 167 healthy individuals were involved as the control group. NP diagnosis was made by nasal endoscopy and paranasal sinus computed tomography (CT). NP was defined as the presence of endoscopically visible bilateral polyps originated from the middle meatus to the nasal cavity and affecting more than one paranasal sinus confirmed by CT. Blood was collected in EDTA-containing tubes and DNA was extracted from the leukocytes. The genotyping of polymorphisms of GSTT1, GSTM1, and GSTP1 were done using real time polymerase chain reaction. Chi-square (chi(2)) and Fisher's (F) exact tests were used for statistical evaluation. A 2-fold increased risk of NP could be found in individuals with the GSTT1 null genotype (OR = 2.03, 95% CI = 1.03-4.011). The distribution of GSTM1 null genotypes was not significantly different between the NP patients and controls and there was also no significance between the GSTP1 genotypes and NP. In conclusion, GST gene polymorphisms may be important in pathogenesis of NP. Additional studies which include larger study groups in different geographic localizations may be more useful to evaluate association with GST polymorphism and NP.
authors.affiliation: Department of Otorhinolaryngology, School of Medicine, Mersin University, Ihsaniye mahallesi 123. cad. 4931 sokak, 33079 Mersin, Turkey. cozcan@mersin.edu.tr
authors.forename: Cengiz
authors.initials: C
authors.lastname: Ozcan
authors.forename: Lülüfer
authors.initials: L
authors.lastname: Tamer
authors.forename: Nurcan Aras
authors.initials: NA
authors.lastname: Ates
authors.forename: Kemal
authors.initials: K
authors.lastname: Görür
mesh_terms: D000328:Adult; D004247:DNA / Q000235:genetics*; D004724:Endoscopy; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D009298:Nasal Polyps / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014057:Tomography, X-Ray Computed
chemical_list: D004247:DNA; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human
references.citation: Curr Opin Otolaryngol Head Neck Surg. 2008 Feb;16(1):18-21
references.pmid: 18197016
references.citation: Mol Aspects Med. 1991;12(2):137-47
references.pmid: 2072822
references.citation: Thorax. 2008 Apr;63(4):322-8
references.pmid: 18057098
references.citation: Allergy. 2006 Feb;61(2):265-7
references.pmid: 16409207
references.citation: Am J Otolaryngol. 2009 Jan-Feb;30(1):24-32
references.pmid: 19027509
references.citation: Rhinology. 2006 Mar;44(1):14-8
references.pmid: 16550944
references.citation: Allergy. 2006 Nov;61(11):1290-8
references.pmid: 17002704
references.citation: Laryngoscope. 2006 Mar;116(3):417-22
references.pmid: 16540901
references.citation: Clin Exp Allergy. 2005 Sep;35(9):1155-61
references.pmid: 16164441
references.citation: Curr Opin Allergy Clin Immunol. 2003 Feb;3(1):1-6
references.pmid: 12582307
references.citation: Eur Arch Otorhinolaryngol. 2005 Jan;262(1):55-60
references.pmid: 15004706
references.citation: Carcinogenesis. 1998 Feb;19(2):275-80
references.pmid: 9498276
references.citation: Free Radic Res. 1995 Mar;22(3):193-207
references.pmid: 7757196
references.citation: Adv Pharmacol. 1994;27:37-69
references.pmid: 8068560
references.citation: Cell Biochem Funct. 2006 Sep-Oct;24(5):461-5
references.pmid: 16287205
references.citation: Int J Pediatr Otorhinolaryngol. 2003 Apr;67(4):383-8
references.pmid: 12663110
references.citation: Auris Nasus Larynx. 2009 Aug;36(4):427-30
references.pmid: 19010623
references.citation: Cell Mol Biol (Noisy-le-grand). 2008 Oct 28;54 Suppl:OL1043-5
references.pmid: 18954553
references.citation: J Laryngol Otol. 1992 Dec;106(12):1084-5
references.pmid: 1487667
references.citation: Pharmacol Res. 2002 Jan;45(1):5-9
references.pmid: 11820854
references.citation: Arch Otolaryngol Head Neck Surg. 1994 Sep;120(9):993-6
references.pmid: 8074828
references.citation: Respirology. 2004 Nov;9(4):493-8
references.pmid: 15612961
references.citation: J Laryngol Otol. 2003 Jan;117(1):1-9
references.pmid: 12590849
references.citation: Eur Arch Otorhinolaryngol. 2000;257(3):137-9
references.pmid: 10839486
references.citation: Basic Res Cardiol. 2004 May;99(3):223-9
references.pmid: 15088107
references.citation: J Allergy Clin Immunol. 2003 Dec;112(6):1057-63
references.pmid: 14657858
references.citation: Laryngoscope. 2004 Jul;114(7):1200-3
references.pmid: 15235348
references.citation: Mediators Inflamm. 2007;2007:19564
references.pmid: 17497028
references.citation: Otolaryngol Head Neck Surg. 2001 May;124(5):570-2
references.pmid: 11337664