title: Live-cell imaging reveals periarbuscular membrane domains and organelle location in Medicago truncatula roots during arbuscular mycorrhizal symbiosis.
abstract: In the arbuscular mycorrhizal symbiosis, the fungal symbiont colonizes root cortical cells, where it establishes differentiated hyphae called arbuscules. As each arbuscule develops, the cortical cell undergoes a transient reorganization and envelops the arbuscule in a novel symbiosis-specific membrane, called the periarbuscular membrane. The periarbuscular membrane, which is continuous with the plant plasma membrane of the cortical cell, is a key interface in the symbiosis; however, relatively little is known of its composition or the mechanisms of its development. Here, we used fluorescent protein fusions to obtain both spatial and temporal information about the protein composition of the periarbuscular membrane. The data indicate that the periarbuscular membrane is composed of at least two distinct domains, an "arbuscule branch domain" that contains the symbiosis-specific phosphate transporter, MtPT4, and an "arbuscule trunk domain" that contains MtBcp1. This suggests a developmental transition from plasma membrane to periarbuscular membrane, with biogenesis of a novel membrane domain associated with the repeated dichotomous branching of the hyphae. Additionally, we took advantage of available organelle-specific fluorescent marker proteins to further evaluate cells during arbuscule development and degeneration. The three-dimensional data provide new insights into relocation of Golgi and peroxisomes and also illustrate that cells with arbuscules can retain a large continuous vacuolar system throughout development.
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York 14853, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Pumplin
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Harrison
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D046913:Medicago truncatula / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D021962:Membrane Microdomains / Q000378:metabolism*; D008954:Models, Biological; D038821:Mycorrhizae / Q000166:cytology* / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013559:Symbiosis / Q000502:physiology*
chemical_list: D015415:Biomarkers; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins
references.citation: Planta. 2005 Nov;222(4):688-98
references.pmid: 16133217
references.citation: Planta. 2001 Oct;213(6):864-8
references.pmid: 11722122
references.citation: Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11841-3
references.pmid: 11607500
references.citation: Plant Cell. 2005 Apr;17(4):1128-40
references.pmid: 15772281
references.citation: Science. 2000 Sep 15;289(5486):1920-1
references.pmid: 10988069
references.citation: Plant J. 2005 Nov;44(3):516-29
references.pmid: 16236160
references.citation: New Phytol. 1998 Dec;140(4):745-752
references.pmid: 33862958
references.citation: Genes Dev. 2001 May 1;15(9):1115-27
references.pmid: 11331607
references.citation: Nat Rev Microbiol. 2008 Oct;6(10):763-75
references.pmid: 18794914
references.citation: Plant Physiol. 2003 Dec;133(4):1691-701
references.pmid: 14681532
references.citation: Biochim Biophys Acta. 2006 Dec;1763(12):1478-95
references.pmid: 17030442
references.citation: Plant J. 2007 Sep;51(6):1126-36
references.pmid: 17666025
references.citation: Curr Biol. 1996 Mar 1;6(3):325-30
references.pmid: 8805250
references.citation: Plant Physiol. 2005 Apr;137(4):1283-301
references.pmid: 15778460
references.citation: Plant Cell. 2004 Jan;16(1):229-40
references.pmid: 14671020
references.citation: New Phytol. 2007;173(1):11-26
references.pmid: 17176390
references.citation: Trends Plant Sci. 2008 Sep;13(9):492-8
references.pmid: 18701339
references.citation: Electrophoresis. 2004 Feb;25(3):519-31
references.pmid: 14760646
references.citation: Plant Physiol. 2003 Sep;133(1):16-20
references.pmid: 12970469
references.citation: J Cell Biol. 1989 Nov;109(5):2145-56
references.pmid: 2478564
references.citation: Plant Physiol. 2005 Sep;139(1):329-40
references.pmid: 16126866
references.citation: Plant Cell. 2008 May;20(5):1407-20
references.pmid: 18515499
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:361-389
references.pmid: 15012214
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17812-7
references.pmid: 19004783
references.citation: BMC Plant Biol. 2009 Jan 22;9:10
references.pmid: 19161626
references.citation: Plant J. 2007 May;50(3):529-44
references.pmid: 17419842
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1720-5
references.pmid: 17242358
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13324-9
references.pmid: 12271140
references.citation: Plant Cell. 2003 Sep;15(9):2106-23
references.pmid: 12953114
references.citation: Annu Rev Microbiol. 2005;59:19-42
references.pmid: 16153162
references.citation: Plant Cell. 2005 Jun;17(6):1749-63
references.pmid: 15849274
references.citation: Plant Physiol. 2008 Dec;148(4):1985-95
references.pmid: 18931145
references.citation: Protoplasma. 2001;217(4):154-65
references.pmid: 11732307
references.citation: Plant J. 2005 Apr;42(2):236-50
references.pmid: 15807785
references.citation: Science. 1989 Sep 29;245(4925):1499-501
references.pmid: 2571189
references.citation: Plant Cell. 2002 Oct;14(10):2413-29
references.pmid: 12368495
references.citation: Mol Plant Microbe Interact. 2001 Jun;14(6):695-700
references.pmid: 11386364
references.citation: Physiol Plant. 2004 Feb;120(2):319-327
references.pmid: 15032867
references.citation: Planta. 2007 Feb;225(3):541-50
references.pmid: 16944200
references.citation: Annu Rev Cell Dev Biol. 2004;20:481-504
references.pmid: 15473849
references.citation: Plant Cell. 2005 Dec;17(12):3489-99
references.pmid: 16284314
references.citation: Plant Cell. 1996 Oct;8(10):1871-1883
references.pmid: 12239368
references.citation: New Phytol. 2006;171(4):699-718
references.pmid: 16918543
references.citation: J Biol Chem. 2004 Aug 13;279(33):34624-30
references.pmid: 15159398
references.citation: Plant J. 2003 Aug;35(4):545-55
references.pmid: 12904216
references.citation: Plant Physiol. 1999 Dec;121(4):1127-42
references.pmid: 10594100
references.citation: Planta. 2000 Oct;211(5):609-13
references.pmid: 11089672
references.citation: Proteomics. 2006 Apr;6 Suppl 1:S145-55
references.pmid: 16511816