The promoting role of an isolate of dark-septate fungus on its host plant Saussurea involucrata Kar. et Kir.
A dark-septate endophytic (DSE) fungus EF-37 was isolated from the roots of Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant. The molecular identification of the fungus was based on internal transcribed spacer regions and the result showed that EF-37 was congeneric to Mycocentrospora. This study was conducted to clarify the influence of the root endophyte EF-37 on the host plant S. involucrata using material grown in a sterile culture bottle. After cultivation for 40 days, fungal hyphae were found to be branching repeatedly and forming "hyphae nets" in the epidermal layers. Significant differences were detected between the study groups in plant dry weight, plant height, root dry weight, shoot dry weight, and the number of hair root tips. There was a positive effect of endophyte EF-37 on plant root development, with results showing that cortical cells dissolved and formed aerate structures. There was a positive effect of endophyte EF-37 on plant growth, but chlorophyll fluorescence analysis showed that there were no significant differences between the study groups. In addition, analysis of the chemical composition of seedlings showed that the level of rutin was higher in plants cultivated with the EF-37 fungus compared to the controls. This study helps to establish a basis for germplasm conservation and for further investigation of the interaction between dark-septate fungi and this alpine plant.
Wu
Li-qin
LQ
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, People's Republic of China. wulq1999@yahoo.com.cn
Lv
Ya-li
YL
Meng
Zhi-xia
ZX
Chen
Juan
J
Guo
Shun-Xing
SX
D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018533:Biomass; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D018520:Plant Shoots / Q000737:chemistry; D012431:Rutin / Q000032:analysis; D030441:Saussurea / Q000254:growth & development* / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012431:Rutin
Yao Xue Xue Bao. 1980 Jun;15(6):368-70
7457157
Plant Cell Rep. 2007 Mar;26(3):261-5
16988830
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1999 Dec;21(6):460-5
12567494
J Pharm Biomed Anal. 2007 May 9;44(1):288-92
17391889
J Exp Bot. 2000 Apr;51(345):659-68
10938857
Mycorrhiza. 2008 Feb;18(2):79-85
18188612
Planta Med. 1999 Feb;65(1):64-7
10083848
Evolution. 1985 Jul;39(4):783-791
28561359
Planta Med. 1994 Oct;60(5):434-7
7997472
Annu Rev Phytopathol. 1999;37:447-471
11701831
Oecologia. 1991 Sep;88(1):15-21
28312726
World J Microbiol Biotechnol. 1993 May;9(3):342-4
24420039
Fungal Genet Biol. 1998 Jun-Jul;24(1-2):240-51
9742204
Plant Cell Rep. 2006 Jan;24(12):750-4
16136313
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
Plant Physiol. 1987 Jun;84(2):218-24
16665420
Plant Cell Rep. 2003 Jul;21(11):1129-33
12836008
Mycologia. 2007 Mar-Apr;99(2):175-84
17682770
Mycorrhiza. 1998 Feb;7(5):261-5
24578052
Mol Phylogenet Evol. 1996 Oct;6(2):287-94
8899729
Plant Physiol. 1985 Jul;78(3):438-41
16664262
Comput Appl Biosci. 1996 Aug;12(4):357-8
8902363