title: Modic changes and interleukin 1 gene locus polymorphisms in occupational cohort of middle-aged men.
abstract: According to recent systematic reviews, Modic changes are associated with low-back pain. However, their pathophysiology remains largely unknown. A previous study of Northern Finnish males implicated that IL1A and MMP3 polymorphisms play a role in type II Modic changes. The purpose of the current study was to examine the association of IL1 cluster polymorphisms with Modic changes amongst middle-aged men in Southern Finland. The final study sample consisted of 108 men from three different occupations, who underwent magnetic resonance imaging (MRI) with a 0.1 T-scanner. Six single nucleotide polymorphisms (SNP) in the IL1 gene cluster (IL1A c.1-889C>T; IL1B c.3954C>T; IL1RN c.1812G>A; IL1RN c.1887G>C; IL1RN c.11100T>C; IL1RN c.1506G>A) were genotyped with the SNP-TRAP method or by allele-specific primer extension on modified microarray. In all, 45 subjects had Modic changes at one or more disc levels. The presence of the minor allele of IL1A (c.1-889C>T) was associated with these changes (any Modic change p = 0.031, type II changes p = 0.036). The carriers of the T-allele had a 2.5-fold risk of Modic change and the association was independent of the other IL1 gene cluster loci studied. In addition, a minor haplotype, with a frequency of 7.5% in the study population, including the minor alleles of IL1A c.1-889C>T, IL1RN c.1812G>A, and IL1RN c.1506G>A, was significantly associated with Modic changes. This observation is in accordance with the previous finding from a different geographical area, and thus confirms the importance of the IL1A gene in the pathophysiology of Modic changes.
authors.affiliation: Finnish Institute of Occupational Health, Helsinki, Finland. jaro.karppinen@ttl.fi
authors.forename: Jaro
authors.initials: J
authors.lastname: Karppinen
authors.forename: Svetlana
authors.initials: S
authors.lastname: Solovieva
authors.forename: Katariina
authors.initials: K
authors.lastname: Luoma
authors.forename: Raili
authors.initials: R
authors.lastname: Raininko
authors.forename: Päivi
authors.initials: P
authors.lastname: Leino-Arjas
authors.forename: Hilkka
authors.initials: H
authors.lastname: Riihimäki
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D015331:Cohort Studies; D004252:DNA Mutational Analysis; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing; D005838:Genotype; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D007403:Intervertebral Disc / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D055959:Intervertebral Disc Degeneration / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D017116:Low Back Pain / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D011110:Polymorphism, Genetic / Q000235:genetics*; D013131:Spine / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D013166:Spondylitis / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D005819:Genetic Markers; D007375:Interleukin-1
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