Identification of vestibuloocular projection neurons in the developing chicken medial vestibular nucleus.
Biocytin was injected into the oculomotor, trochlear, or abducens nucleus on one side using isolated chicken brainstem preparations or brain slices to identify the medial vestibular nucleus (MVN) neurons projecting to these targets. Oculomotor nucleus injections produced retrogradely labeled neurons in the contralateral ventrolateral MVN (MVN(VL)), with few labeled neurons in the ipsilateral MVN(VL) and rarely in the dorsomedial MVN on either side. Labeled MVN(VL) neurons were identified as stellate (95%) and elongate (5%) cells. Trochlear nucleus injections produced a similar pattern of MVN neuron labeling. Abducens nucleus injections resulted in retrogradely labeled stellate (87%) and elongate (13%) neurons in the MVN(VL), which had smaller cell bodies than those projecting to the oculomotor nucleus. Anteroposteriorly, labeled MVN(VL) neurons were coextensive with the tangential nucleus, with neurons projecting to the oculomotor nucleus distributed lateral to and intermixed with the more medially situated neurons projecting to the abducens nucleus. The fundamental pattern of vestibuloocular projecting neurons was similar at both embryonic ages studied, E16 and E13. In contrast to the case in mammals, where most vestibuloocular projection neurons reside within the MVN, most retrogradely labeled neurons in these chicken preparations were found within the ventrolateral vestibular, descending vestibular, and tangential nuclei. The morphological identification and mapping of vestibuloocular projection neurons in the chicken MVN described here represents the first step in a systematic evaluation of the relationship between avian vestibuloocular neuron structure and function.
Gottesman-Davis
Adria
A
Department of Anatomy and Regenerative Biology, George Washington University Medical Center, Washington, DC 20037, USA.
Peusner
Kenna D
KD
D000818:Animals; D030161:Avian Proteins / Q000378:metabolism; D001933:Brain Stem / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens; D007839:Functional Laterality; D066298:In Vitro Techniques; D008239:Lysine / Q000031:analogs & derivatives; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009434:Neural Pathways / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D056325:Neuronal Tract-Tracers; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D013045:Species Specificity; D014726:Vestibular Nuclei / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism
D030161:Avian Proteins; D008869:Microtubule-Associated Proteins; D056325:Neuronal Tract-Tracers; C013411:biocytin; D008239:Lysine
Brain Res Bull. 2008 Mar 18;75(2-4):419-23
18331909
J Comp Neurol. 1987 Aug 15;262(3):429-45
2443544
J Comp Neurol. 1991 Apr 8;306(2):273-89
1711055
Exp Brain Res. 1988;70(1):181-91
3402563
Brain Behav Evol. 1983;22(2-3):153-63
6303494
J Neurosci. 2007 Feb 28;27(9):2318-30
17329429
Anat Rec. 1998 Aug;251(4):431-8
9713981
J Physiol. 1967 Jan;188(2):31P-32P
6030525
J Comp Neurol. 1988 Feb 1;268(1):13-28
3346381
Exp Brain Res. 1990;83(1):9-21
2073953
Exp Brain Res. 1984;55(3):574-8
6332031
J Comp Neurol. 2000 Sep 11;425(1):97-120
10940945
Prog Neurobiol. 2005 Aug;76(6):349-92
16263204
Prog Brain Res. 2006;151:157-203
16221589
Brain Behav Evol. 1991;37(2):111-24
2054584
J Comp Neurol. 2001 Apr 30;433(2):255-71
11283963
Hear Res. 2000 Nov;149(1-2):91-105
11033249
J Morphol. 1951 Jan;88(1):49-92
24539719
J Comp Neurol. 1978 May 15;179(2):393-405
641224
J Neurophysiol. 2003 Aug;90(2):1182-92
12904504
J Comp Neurol. 1994 Aug 1;346(1):19-42
7962710
Exp Brain Res. 2007 Feb;176(4):665-71
17216144
J Comp Neurol. 1997 Aug 11;384(4):621-33
9259493
J Neurophysiol. 1992 Aug;68(2):471-84
1527570
J Comp Neurol. 1994 Aug 1;346(1):1-18
7962705
J Neurophysiol. 2006 Sep;96(3):1215-26
16775212
J Comp Neurol. 1991 Aug 1;310(1):82-93
1719038
Neuroscience. 1977;2(2):209-27
904775
Eur J Biochem. 1996 May 15;238(1):1-27
8665925
Exp Brain Res. 1980;40(4):468-73
7439286
Dev Dyn. 1994 Oct;201(2):191-202
7873790
J Neurosci Res. 2008 Mar;86(4):832-44
17941057
J Comp Neurol. 1987 May 1;259(1):140-9
3584553
J Hirnforsch. 1991;32(2):203-9
1791300
Acta Anat (Basel). 1967;66(3):420-45
6058705
J Neurophysiol. 2006 May;95(5):3012-23
16436481
Brain Res Bull. 2002 Feb-Mar 1;57(3-4):307-12
11922978
Exp Brain Res. 1986;63(1):35-48
3732448
J Comp Neurol. 1989 Apr 22;282(4):523-34
2723150
J Neurophysiol. 1983 Dec;50(6):1265-80
6319618
J Physiol Paris. 2003 Jan;97(1):17-25
14706687
J Comp Neurol. 1988 May 15;271(3):451-60
2454968
J Physiol. 2000 Mar 1;523 Pt 2:413-24
10699085
Neuroscience. 2006 Aug 25;141(2):711-726
16690214
Neuroscience. 1977;2(2):189-207
904774
J Comp Neurol. 1990 Feb 8;292(2):231-45
2319011
Neuroscience. 2004;127(1):81-90
15219671
J Comp Neurol. 2007 May 1;502(1):19-37
17335047
J Comp Neurol. 1990 Jul 8;297(2):283-97
2164534
J Comp Neurol. 1990 Jun 8;296(2):324-41
2358540
J Neurosci Methods. 1996 Dec;70(1):91-102
8982986