title: Muscarinic acetylcholine receptor subtypes in the male reproductive tract: expression and function in rat efferent ductules and epididymis.
abstract: In mammals, at least five different muscarinic acetylcholine receptor subtypes (mAChRs; M(1)-M(5)) are known to be widely expressed and distributed in different tissues from different species. They mediate distinct physiological functions according to their location and receptor subtype. Multiple events are associated with the regulation of intracellular signaling by mAChRs, and a coordinated balance of the molecular mechanisms governing receptor signaling, desensitization, resensitization, and mitogenic signaling is known to occur in various cell types. Most of the actions of acetylcholine (ACh) in the male reproductive tract are induced by its effects on mAChRs, but the role of specific mAChR subtypes on male reproductive function and fertility are still not well understood. The rat efferent ductules and epididymis are androgen-dependent tissues of the male reproductive tract, with important roles in the process to form a viable and fertile sperm. In the present study, aspects of the expression, localization, and potential function of mAChR subtypes in rat efferent ductules and epididymis are reviewed. Furthermore, evidences for the implication of mAChRs in the regulation of protein synthesis and secretion in these tissues are presented. Taken together, the studies contribute to our understanding about physiological aspects of mAChR and mechanisms by which the cholinergic system affects male reproduction.
authors.affiliation: Section of Experimental Endocrinology, Department of Pharmacology, Universidade Federal de São Paulo-Escola Paulista de Medicina, INFAR, Vila Clementino, São Paulo, São Paulo 04044-020, Brazil. avellar@unifesp.br
authors.forename: Maria Christina W
authors.initials: MC
authors.lastname: Avellar
authors.forename: Erica R
authors.initials: ER
authors.lastname: Siu
authors.forename: Fabiana
authors.initials: F
authors.lastname: Yasuhara
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Maróstica
authors.forename: Catarina S
authors.initials: CS
authors.lastname: Porto
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000728:Androgens / Q000378:metabolism; D000818:Animals; D004822:Epididymis / Q000166:cytology / Q000294:innervation / Q000378:metabolism*; D004847:Epithelial Cells / Q000502:physiology; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology; D021122:Protein Subunits / Q000378:metabolism; D051381:Rats; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology; D013737:Testis / Q000166:cytology / Q000294:innervation / Q000378:metabolism*
chemical_list: D000728:Androgens; D021122:Protein Subunits; D011976:Receptors, Muscarinic; D000109:Acetylcholine
references.citation: Pharmacol Rev. 1998 Jun;50(2):279-90
references.pmid: 9647869
references.citation: J Androl. 2007 Jan-Feb;28(1):136-49
references.pmid: 16988324
references.citation: Mol Reprod Dev. 2005 Oct;72(2):250-8
references.pmid: 15948184
references.citation: Gen Pharmacol. 1994 Dec;25(8):1643-7
references.pmid: 7721040
references.citation: Gen Pharmacol. 1995 Mar;26(2):387-91
references.pmid: 7590092
references.citation: Nat Rev Drug Discov. 2007 Sep;6(9):721-33
references.pmid: 17762886
references.citation: Br J Pharmacol. 2008 Aug;154(8):1558-71
references.pmid: 18500366
references.citation: Anim Reprod Sci. 2004 Jul;82-83:321-39
references.pmid: 15271463
references.citation: J Investig Dermatol Symp Proc. 1997 Aug;2(1):41-8
references.pmid: 9487015
references.citation: Br J Pharmacol. 1991 Feb;102(2):540-4
references.pmid: 1849774
references.citation: Biol Reprod. 2004 Aug;71(2):410-6
references.pmid: 15056565
references.citation: Theriogenology. 2005 Jan 15;63(2):319-41
references.pmid: 15626402
references.citation: Invest Urol. 1978 Jan;15(4):288-90
references.pmid: 627473
references.citation: Biol Reprod. 1992 Oct;47(4):588-97
references.pmid: 1391346
references.citation: Microsc Res Tech. 1994 Dec 15;29(6):432-67
references.pmid: 7873793
references.citation: Life Sci. 2005 Jun 24;77(6):656-69
references.pmid: 15921997
references.citation: J Reprod Fertil. 1965 Oct;10(2):209-20
references.pmid: 5836268
references.citation: Reprod Toxicol. 2002 Nov-Dec;16(6):757-65
references.pmid: 12401503
references.citation: Prostate. 1991;18(4):289-301
references.pmid: 1676157
references.citation: Mol Reprod Dev. 1991 Jan;28(1):55-61
references.pmid: 1994980
references.citation: Am J Anat. 1967 Jul;121(1):1-14
references.pmid: 6069971
references.citation: Endocrinology. 1987 Nov;121(5):1678-88
references.pmid: 3665841
references.citation: J Neurophysiol. 1987 Mar;57(3):702-11
references.pmid: 3559698
references.citation: Endocrinology. 2001 Nov;142(11):4701-10
references.pmid: 11606435
references.citation: Biochem Pharmacol. 1981 Jun 15;30(12):1521-8
references.pmid: 7271845
references.citation: Biochem Pharmacol. 1976 Jul 15;25(14):1617-22
references.pmid: 945986
references.citation: Eur J Pharmacol. 1998 Feb 19;343(2-3):151-6
references.pmid: 9570462
references.citation: Biol Reprod. 1981 Dec;25(5):1099-117
references.pmid: 7326301
references.citation: Adv Exp Med Biol. 1995;377:87-101
references.pmid: 7484449
references.citation: Chem Biol Interact. 1984 Apr;49(1-2):39-53
references.pmid: 6722939
references.citation: Arch Int Pharmacodyn Ther. 1962 Aug 1;138:354-63
references.pmid: 13943237
references.citation: Pharmacol Ther. 2002 Apr-May;94(1-2):93-112
references.pmid: 12191596
references.citation: Science. 1987 Jul 31;237(4814):527-32
references.pmid: 3037705
references.citation: J Androl. 2002 May-Jun;23(3):374-83
references.pmid: 12002439
references.citation: Annu Rev Pharmacol Toxicol. 2004;44:423-50
references.pmid: 14744253
references.citation: Br J Pharmacol. 2000 Jul;130(5):1013-20
references.pmid: 10882385
references.citation: Acta Anat (Basel). 1985;121(1):59-62
references.pmid: 3969824
references.citation: J Reprod Fertil. 1981 Sep;63(1):205-9
references.pmid: 6115943
references.citation: J Cell Physiol. 1971 Feb;77(1):7-15
references.pmid: 4323201
references.citation: J Reprod Fertil Suppl. 1998;53:119-36
references.pmid: 10645272
references.citation: J Auton Pharmacol. 2000 Aug;20(4):193-206
references.pmid: 11260358
references.citation: Proc Natl Acad Sci U S A. 1991 May 1;88(9):3676-80
references.pmid: 2023918
references.citation: Life Sci. 1997;60(13-14):963-8
references.pmid: 9121362
references.citation: Biol Reprod. 1991 Jun;44(6):1171-6
references.pmid: 1873391
references.citation: Pharmacol Ther. 2008 Jul;119(1):33-43
references.pmid: 18558434
references.citation: J Toxicol Sci. 2005 Aug;30(3):207-12
references.pmid: 16141654
references.citation: Biol Reprod. 2001 Oct;65(4):1120-6
references.pmid: 11566733
references.citation: Pharmacol Ther. 1998 Jan;77(1):59-79
references.pmid: 9500159
references.citation: Cell Tissue Res. 2006 Jan;323(1):157-66
references.pmid: 16160857
references.citation: J Urol. 1989 May;141(5):1225-9
references.pmid: 2709516
references.citation: Annu Rev Pharmacol Toxicol. 1990;30:633-73
references.pmid: 2188581
references.citation: Mol Cell Endocrinol. 2006 Mar 9;247(1-2):192-8
references.pmid: 16481100
references.citation: Endocrinology. 2004 May;145(5):2498-506
references.pmid: 14764638
references.citation: Life Sci. 2004 Aug 20;75(14):1761-73
references.pmid: 15268975
references.citation: Auton Autacoid Pharmacol. 2006 Jul;26(3):219-33
references.pmid: 16879488
references.citation: Biol Reprod. 1978 Sep;19(2):271-9
references.pmid: 719088
references.citation: Z Zellforsch Mikrosk Anat. 1968;90(1):81-95
references.pmid: 5722959
references.citation: J Urol. 1985 Jul;134(1):179-82
references.pmid: 4009819
references.citation: Cell Biol Int. 2006 Sep;30(9):741-6
references.pmid: 16814576
references.citation: J Androl. 1998 Jan-Feb;19(1):1-4
references.pmid: 9537285
references.citation: Auton Neurosci. 2001 Sep 17;92(1-2):1-10
references.pmid: 11570697
references.citation: Brain Res. 2003 Oct 31;989(1):52-7
references.pmid: 14519511
references.citation: Biochem Med Metab Biol. 1989 Aug;42(1):66-70
references.pmid: 2775563
references.citation: J Reprod Fertil. 1984 May;71(1):181-8
references.pmid: 6144794
references.citation: Dev Biol. 1982 May;91(1):121-30
references.pmid: 7201425
references.citation: Urol Res. 1997;25(6):433-8
references.pmid: 9443654
references.citation: Cell Tissue Res. 1976 Apr 9;167(4):515-25
references.pmid: 1268923