Optimization of lipase-catalyzed glucose ester synthesis in ionic liquids.
Lipase-catalyzed esterification of glucose with fatty acids in ionic liquids (ILs) mixture was investigated by using supersaturated glucose solution. The effect of ILs mixture ratio, substrate ratio, lipase content, and temperature on the activity and stability of lipase was also studied. The highest yield of sugar ester was obtained in a mixture of 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TfO]) and 1-methyl-3-octylimidazolium bis[(trifluoromethyl)-sulfonyl]amide ([Omim][Tf(2)N]) with a volume ratio of 9:1, while Novozym 435 (Candida antarctica type B lipase immobilized on acrylic resin) showed the optimal stability and activity in a mixture of [Bmim][TfO] and [Omim][Tf(2)N] with a 1:1 volume ratio. Reuse of lipase and ILs was successfully carried out at the optimized reaction conditions. After 5 times reuse of Novozym 435 and ILs, 78% of initial activity was remained.
Ha
Sung Ho
SH
ERC for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea. shha@inha.ac.kr
Hiep
Nguyen Minh
NM
Lee
Sang Hyun
SH
Koo
Yoon-Mo
YM
D002175:Candida / Q000201:enzymology; D002384:Catalysis; D004800:Enzymes, Immobilized; D004952:Esters / Q000138:chemical synthesis / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry; D007477:Ions; D008049:Lipase / Q000737:chemistry*; D008698:Mesylates / Q000737:chemistry
C532403:1-butyl-3-methylimidazolium trifluoromethanesulfonate; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D007093:Imidazoles; D007477:Ions; D008698:Mesylates; C093667:Novozyme 435; D008049:Lipase; C431349:lipase B, Candida antarctica; D005947:Glucose