title: Genome mining in Sorangium cellulosum So ce56: identification and characterization of the homologous electron transfer proteins of a myxobacterial cytochrome P450.
abstract: Myxobacteria, especially members of the genus Sorangium, are known for their biotechnological potential as producers of pharmaceutically valuable secondary metabolites. The biosynthesis of several of those myxobacterial compounds includes cytochrome P450 activity. Although class I cytochrome P450 enzymes occur wide-spread in bacteria and rely on ferredoxins and ferredoxin reductases as essential electron mediators, the study of these proteins is often neglected. Therefore, we decided to search in the Sorangium cellulosum So ce56 genome for putative interaction partners of cytochromes P450. In this work we report the investigation of eight myxobacterial ferredoxins and two ferredoxin reductases with respect to their activity in cytochrome P450 systems. Intriguingly, we found not only one, but two ferredoxins whose ability to sustain an endogenous So ce56 cytochrome P450 was demonstrated by CYP260A1-dependent conversion of nootkatone. Moreover, we could demonstrate that the two ferredoxins were able to receive electrons from both ferredoxin reductases. These findings indicate that S. cellulosum can alternate between different electron transport pathways to sustain cytochrome P450 activity.
authors.affiliation: Department of Biochemistry, Saarland University, D-66041 Saarbrücken, Germany.
authors.forename: Kerstin Maria
authors.initials: KM
authors.lastname: Ewen
authors.forename: Frank
authors.initials: F
authors.lastname: Hannemann
authors.forename: Yogan
authors.initials: Y
authors.lastname: Khatri
authors.forename: Olena
authors.initials: O
authors.lastname: Perlova
authors.forename: Reinhard
authors.initials: R
authors.lastname: Kappl
authors.forename: Daniel
authors.initials: D
authors.lastname: Krug
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hüttermann
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
authors.forename: Rita
authors.initials: R
authors.lastname: Bernhardt
mesh_terms: D001709:Biotechnology / Q000379:methods; D019295:Computational Biology / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D005288:Ferredoxins / Q000737:chemistry; D005292:Ferricyanides / Q000737:chemistry; D005415:Flavins / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D005821:Genetic Techniques; D016680:Genome, Bacterial; D005982:Glutathione Transferase / Q000378:metabolism; D007700:Kinetics; D009231:Myxococcales / Q000235:genetics*; D017434:Protein Structure, Tertiary
chemical_list: D005288:Ferredoxins; D005292:Ferricyanides; D005415:Flavins; C007931:hexacyanoferrate III; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase
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