title: Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine.
abstract: The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Korea.
authors.forename: Juyeon
authors.initials: J
authors.lastname: Lee
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Jihoon
authors.initials: J
authors.lastname: Kim
authors.forename: Chanwoo
authors.initials: C
authors.lastname: Jung
authors.forename: Keun-Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Joo
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D002908:Chronic Disease; D037502:Galectin 3 / Q000378:metabolism*; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000382:microbiology / Q000473:pathology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000254:growth & development / Q000302:isolation & purification; D010283:Paratuberculosis / Q000473:pathology*; D012319:RNA Polymerase II / Q000235:genetics
chemical_list: D037502:Galectin 3; D012319:RNA Polymerase II
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