title: Temporal controls of the asymmetric cell division cycle in Caulobacter crescentus.
abstract: The asymmetric cell division cycle of Caulobacter crescentus is orchestrated by an elaborate gene-protein regulatory network, centered on three major control proteins, DnaA, GcrA and CtrA. The regulatory network is cast into a quantitative computational model to investigate in a systematic fashion how these three proteins control the relevant genetic, biochemical and physiological properties of proliferating bacteria. Different controls for both swarmer and stalked cell cycles are represented in the mathematical scheme. The model is validated against observed phenotypes of wild-type cells and relevant mutants, and it predicts the phenotypes of novel mutants and of known mutants under novel experimental conditions. Because the cell cycle control proteins of Caulobacter are conserved across many species of alpha-proteobacteria, the model we are proposing here may be applicable to other genera of importance to agriculture and medicine (e.g., Rhizobium, Brucella).
authors.affiliation: Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Li
authors.forename: Paul
authors.initials: P
authors.lastname: Brazhnik
authors.forename: Bruno
authors.initials: B
authors.lastname: Sobral
authors.forename: John J
authors.initials: JJ
authors.lastname: Tyson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016935:Caulobacter crescentus / Q000166:cytology / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D015870:Gene Expression; D020407:Internet; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins
references.citation: EMBO J. 2002 Sep 2;21(17):4420-8
references.pmid: 12198144
references.citation: Annu Rev Genet. 1999;33:423-48
references.pmid: 10690414
references.citation: J Theor Biol. 1991 Aug 7;151(3):359-66
references.pmid: 1943147
references.citation: J Bacteriol. 1987 Nov;169(11):5231-40
references.pmid: 3667530
references.citation: Science. 2003 Sep 26;301(5641):1874-7
references.pmid: 14512618
references.citation: J Biotechnol. 2001 Sep 13;91(1):1-34
references.pmid: 11522360
references.citation: Science. 2002 Dec 6;298(5600):1942-6
references.pmid: 12471245
references.citation: Mol Syst Biol. 2007;3:131
references.pmid: 17667953
references.citation: Genes Dev. 2008 Jan 15;22(2):212-25
references.pmid: 18198338
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13160-5
references.pmid: 12237413
references.citation: J Bacteriol. 2007 Nov;189(22):8308-20
references.pmid: 17827294
references.citation: J Bacteriol. 1999 May;181(10):3039-50
references.pmid: 10322004
references.citation: J Bacteriol. 2006 Apr;188(7):2473-82
references.pmid: 16547034
references.citation: J Theor Biol. 1989 Oct 23;140(4):453-77
references.pmid: 2559257
references.citation: Nat Rev Mol Cell Biol. 2001 Dec;2(12):908-16
references.pmid: 11733770
references.citation: Genes Dev. 2009 Jan 1;23(1):93-104
references.pmid: 19136627
references.citation: Mol Microbiol. 2007 May;64(4):938-52
references.pmid: 17501919
references.citation: EMBO J. 2006 Jan 25;25(2):346-56
references.pmid: 16395331
references.citation: Cell. 1999 Apr 2;97(1):111-20
references.pmid: 10199407
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):10935-40
references.pmid: 16829582
references.citation: J Math Biol. 1980 Jun;9(4):389-98
references.pmid: 7005363
references.citation: Mol Microbiol. 2003 Feb;47(4):929-41
references.pmid: 12581350
references.citation: Genetics. 1991 Nov;129(3):623-30
references.pmid: 1752411
references.citation: J Theor Biol. 2004 Oct 21;230(4):563-79
references.pmid: 15363676
references.citation: PLoS Comput Biol. 2008 Jan;4(1):e9
references.pmid: 18225942
references.citation: J Cell Sci. 2007 Oct 15;120(Pt 20):3501-7
references.pmid: 17928306
references.citation: Annu Rev Genet. 2007;41:429-41
references.pmid: 18076330
references.citation: Cell Cycle. 2006 Mar;5(5):522-9
references.pmid: 16552176
references.citation: Mol Microbiol. 1998 May;28(3):421-34
references.pmid: 9632248
references.citation: J Cell Biochem. 2006 Feb 15;97(3):459-73
references.pmid: 16288461
references.citation: Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):630-4
references.pmid: 8421698
references.citation: Curr Biol. 2006 Oct 24;16(20):R881-4
references.pmid: 17055971
references.citation: Biophys Chem. 1998 May 5;72(1-2):169-84
references.pmid: 9652093
references.citation: Cell. 2006 Jul 14;126(1):147-62
references.pmid: 16839883
references.citation: Science. 2000 Dec 15;290(5499):2144-8
references.pmid: 11118148
references.citation: J Bacteriol. 1989 Jan;171(1):392-401
references.pmid: 2536661
references.citation: Bioessays. 2006 Apr;28(4):355-61
references.pmid: 16547950
references.citation: Nature. 2006 Dec 14;444(7121):899-904
references.pmid: 17136100
references.citation: Acta Biochim Pol. 2005;52(1):1-34
references.pmid: 15827603
references.citation: Trends Biotechnol. 2002 Nov;20(11):467-72
references.pmid: 12413821
references.citation: Mol Microbiol. 2004 Oct;54(1):60-74
references.pmid: 15458405
references.citation: Dev Cell. 2003 Jul;5(1):149-59
references.pmid: 12852859
references.citation: Science. 2004 May 14;304(5673):983-7
references.pmid: 15087506
references.citation: Biophys J. 2006 Jun 15;90(12):4361-79
references.pmid: 16581849
references.citation: J Theor Biol. 1995 Oct 7;176(3):411-30
references.pmid: 8538219
references.citation: PLoS One. 2008 Feb 06;3(2):e1555
references.pmid: 18253502
references.citation: Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):120-5
references.pmid: 9419339
references.citation: Chaos. 2001 Mar;11(1):277-286
references.pmid: 12779461
references.citation: Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4632-7
references.pmid: 11930012
references.citation: Biophys Chem. 1998 May 5;72(1-2):185-200
references.pmid: 9652094
references.citation: Cell. 2006 Feb 10;124(3):535-47
references.pmid: 16469700
references.citation: EMBO J. 2000 Sep 1;19(17):4503-12
references.pmid: 10970844
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4681-6
references.pmid: 11287652
references.citation: EMBO J. 2006 Jan 25;25(2):377-86
references.pmid: 16395329
references.citation: Mol Microbiol. 2005 Jun;56(6):1467-80
references.pmid: 15916599
references.citation: Proc Natl Acad Sci U S A. 1989 Jan;86(1):119-23
references.pmid: 2911562
references.citation: Mol Microbiol. 2001 Nov;42(3):741-55
references.pmid: 11722739
references.citation: Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):17111-6
references.pmid: 17942674
references.citation: Mol Microbiol. 2002 Aug;45(3):605-16
references.pmid: 12139609
references.citation: Mol Biol Cell. 2004 Aug;15(8):3841-62
references.pmid: 15169868
references.citation: J Cell Sci. 1993 Dec;106 ( Pt 4):1153-68
references.pmid: 8126097
references.citation: Cell. 1996 Jan 12;84(1):83-93
references.pmid: 8548829
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11340-5
references.pmid: 18685108
references.citation: Annu Rev Microbiol. 2003;57:225-47
references.pmid: 14527278
references.citation: FEMS Microbiol Rev. 2000 Apr;24(2):177-91
references.pmid: 10717313
references.citation: J Biol Rhythms. 2007 Dec;22(6):542-53
references.pmid: 18057329
references.citation: Nat Rev Microbiol. 2004 Apr;2(4):325-37
references.pmid: 15031731
references.citation: Mol Cell. 2002 Aug;10(2):427-34
references.pmid: 12191487
references.citation: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8235-40
references.pmid: 12824468
references.citation: Microbiol Rev. 1991 Sep;55(3):459-75
references.pmid: 1943997
references.citation: Biotechnol Bioeng. 2004 Dec 5;88(5):575-84
references.pmid: 15470709
references.citation: J Bacteriol. 1994 Jan;176(2):401-8
references.pmid: 8288535
references.citation: Cell. 1997 Mar 7;88(5):675-84
references.pmid: 9054507
references.citation: Res Microbiol. 1991 Feb-Apr;142(2-3):161-7
references.pmid: 1925015
references.citation: Cell. 2008 May 2;133(3):452-61
references.pmid: 18455986
references.citation: Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):85-6
references.pmid: 9419330
references.citation: Mol Cell. 1999 Nov;4(5):683-94
references.pmid: 10619016
references.citation: Results Probl Cell Differ. 2006;42:227-56
references.pmid: 16903213
references.citation: Mol Microbiol. 2003 Mar;47(5):1279-90
references.pmid: 12603734
references.citation: J Bacteriol. 1999 Jul;181(13):3967-73
references.pmid: 10383964
references.citation: J Theor Biol. 1984 Nov 7;111(1):183-99
references.pmid: 6392751
references.citation: EMBO J. 2002 Sep 16;21(18):4969-77
references.pmid: 12234936
references.citation: Cell. 1997 Aug 8;90(3):415-24
references.pmid: 9267022
references.citation: Annu Rev Biochem. 2003;72:367-94
references.pmid: 12651741
references.citation: Mol Microbiol. 2001 May;40(4):795-803
references.pmid: 11401687
references.citation: Mol Microbiol. 2005 Dec;58(5):1340-53
references.pmid: 16313620
references.citation: EMBO J. 1998 Oct 1;17(19):5658-69
references.pmid: 9755166
references.citation: Trends Microbiol. 2007 Mar;15(3):101-8
references.pmid: 17275310