title: CnrN regulates Dictyostelium group size using a counting factor-independent mechanism.
abstract: One of the simplest examples of a complex behavior is the aggregation of solitary Dictyostelium discoideum amoebae to form a 20,000-cell fruiting body. A field of starving amoebae first breaks up into territories. In each territory, the cells form a spider-like pattern of streams of cells. As part of a negative feedback loop, counting factor (CF), a secreted protein complex whose concentration increases with the size of the stream, prevents over-sized fruiting bodies from being formed by increasing cell motility and decreasing cell-cell adhesion, which causes the breakup of excessively large streams. Cells lacking the phosphatase CnrN (cnrN(-) cells) form small aggregation territories and few streams.1 In this report, we present computer simulations that suggest that in the absence of stream formation, CF should be unable to affect group size. As predicted, cnrN(-) group size is insensitive to the addition or depletion of CF. Together, the data indicate that CnrN regulates group size by regulating both the break-up of a field of cells into aggregation territories and stream formation during development, and that CnrN-mediated and CF-mediated group size regulation use different mechanisms.
authors.affiliation: Department of Biochemistry and Cell Biology; Rice University; Houston, Texas USA.
authors.forename: Yitai
authors.initials: Y
authors.lastname: Tang
authors.forename: Richard H
authors.initials: RH
authors.lastname: Gomer
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