Gene silencing to investigate the roles of receptor-like proteins in Arabidopsis.
Receptor-like proteins (RLPs) are cell surface receptors that play important roles in various processes. In several plant species RLPs have been found to play a role in disease resistance, including the tomato Cf and Ve proteins and the apple HcrVf proteins that mediate resistance against the fungal pathogens Cladosporium fulvum, Verticillium spp., and Venturia inaequalis, respectively. The Arabidopsis genome contains 57 AtRLP genes. Two of these, CLV2 (AtRLP10) and TMM (AtRLP17), have well-characterized functions in meristem and stomatal development, respectively, while AtRLP52 is required for defense against powdery mildew. We recently reported the assembly of a genome-wide collection of T-DNA insertion lines for the Arabidopsis AtRLP genes. This collection was functionally analyzed with respect to plant growth, development and sensitivity to various stress responses including pathogen susceptibility. Only few new phenotypes were discovered; while AtRLP41 was found to mediate abscisic acid sensitivity, AtRLP30 (and possibly AtRLP18) was found to be required for full non-host resistance to a bacterial pathogen. Possibly, identification of novel phenotypes is obscured by functional redundancy. Therefore, RNA interference (RNAi) to target the expression of multiple AtRLP genes simultaneously was employed followed by functional analysis of the RNAi lines.
Ellendorff
Ursula
U
Laboratory of Phytopathology, Wageningen University, Wageningen The Netherlands.
Zhang
Zhao
Z
Thomma
Bart Phj
BP
Plant Physiol. 2008 Jun;147(2):503-17
18434605
Science. 2002 May 31;296(5573):1697-700
12040198
Curr Opin Immunol. 2001 Feb;13(1):63-8
11154919
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6511-5
11331751
Plant Mol Biol. 2000 Apr;42(6):819-32
10890530
Genes Dev. 2001 Oct 15;15(20):2755-66
11641280
Mol Plant Microbe Interact. 2006 Jun;19(6):567-76
16776290
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
Plant Physiol. 2005 Jun;138(2):1027-36
15923325
Mol Plant Pathol. 2005 Jul 1;6(4):379-93
20565665
Mol Plant Pathol. 2005 Jan 1;6(1):85-97
20565641
Plant Physiol. 2005 Jun;138(2):611-23
15955925
Annu Rev Entomol. 2006;51:663-89
16332227
Trends Genet. 2004 Mar;20(3):116-22
15049302
Plant Cell. 1999 Oct;11(10):1925-34
10521522
Mol Plant Microbe Interact. 2008 Apr;21(4):448-58
18321190
Plant J. 1998 Dec;16(6):735-43
10069079
Plant Cell. 2000 Nov;12(11):2075-86
11090210
Plant Cell. 2004 Jun;16(6):1604-15
15155877
Annu Rev Phytopathol. 1999;37:335-367
11701827
Mol Plant Pathol. 2006 Mar 1;7(2):71-86
20507429