Tetraploidization of diploid Dioscorea results in activation of the antioxidant defense system and increased heat tolerance.
Polyploidy is reported to show increased tolerance to environmental stress. In this work, tetraploid plants of Dioscorea zingiberensis were obtained by colchicine treatment of shoots propagated in vitro. The highest tetraploid induction rate was achieved by treatment with 0.15% colchicine for 24h. Diploid and tetraploid plants were exposed to normal (28 degrees C) and high temperature (42 degrees C) for 5d during which physiological indices were measured. Compared with diploid plants, relative electrolyte leakage and contents of malondialdehyde, superoxide anions and hydrogen peroxide were lower in tetraploids, while activities of antioxidant enzymes, such as superoxide dismutase, peroxidase, catalase, ascorbate peroxidase and glutathione reductase, were stimulated and antioxidants (ascorbic acid and glutathione) were maintained at high concentrations. These results indicate that tetraploid plants possess a stronger antioxidant defense system and increased heat tolerance.
Zhang
Xiao-Yi
XY
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Hu
Chun-Gen
CG
Yao
Jia-Ling
JL
D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D027742:Dioscorea / Q000235:genetics* / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D011123:Polyploidy; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D001205:Ascorbic Acid