title: Hypothalamic-pituitary-adrenal axis and smoking and drinking onset among adolescents: the longitudinal cohort TRacking Adolescents' Individual Lives Survey (TRAILS).
abstract: OBJECTIVE
  We examined within a prospective longitudinal study whether cortisol levels were associated with smoking or drinking behaviours, taking parental substance use into account.
  
  
  METHODS
  The influence of parental substance use on cortisol levels of their adolescent offspring at age 10-12 years was examined. Next, cortisol levels of adolescents who initiated smoking or drinking at the first data collection (age 10-12) were compared to non-users. Finally, we examined whether cortisol levels could predict new onset and frequency of smoking and drinking 2 years later.
  
  
  METHODS
  First and second assessment data of the TRacking Adolescents' Individual Lives Survey (TRAILS) were used, including 1768 Dutch adolescents aged 10-12 years, who were followed-up across a period of 2 years.
  
  
  METHODS
  Cortisol was measured in saliva samples at awakening, 30 minutes later, and at 8 p.m. at age 10-12. Self-reported substance use at age 10-12 and 13-14, and parental self-reported substance use were used.
  
  
  RESULTS
  Only maternal substance use was related to slightly lower adolescent cortisol levels at 8 p.m. Both maternal and paternal substance use were associated with adolescent smoking and drinking at age 13-14, although fathers' use only predicted the amount used and not the chance of ever use. Finally, higher cortisol levels were related moderately to current smoking and future frequency of smoking, but not to alcohol use.
  
  
  CONCLUSIONS
  In a general population, parental heavy substance use does not seem to affect cortisol levels consistently in their offspring. We found some evidence for higher, instead of lower, hypothalamic-pituitary-adrenal axis activity as a predictor of smoking in early adolescence.
authors.affiliation: Department of Child and Adolescent Psychiatry, Erasmus Medical Center, 3000 CB Rotterdam, the Netherlands. a.c.huizink@erasmusmc.nl
authors.forename: Anja C
authors.initials: AC
authors.lastname: Huizink
authors.forename: Kirstin
authors.initials: K
authors.lastname: Greaves-Lord
authors.forename: Albertine J
authors.initials: AJ
authors.lastname: Oldehinkel
authors.forename: Johan
authors.initials: J
authors.lastname: Ormel
authors.forename: Frank C
authors.initials: FC
authors.lastname: Verhulst
mesh_terms: D000293:Adolescent; D000294:Adolescent Behavior / Q000502:physiology; D000428:Alcohol Drinking / Q000453:epidemiology*; D002648:Child; D004812:Epidemiologic Methods; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology*; D008297:Male; D009426:Netherlands / Q000453:epidemiology; D010287:Parent-Child Relations; D016487:Parenting; D010290:Parents; D010913:Pituitary-Adrenal System / Q000502:physiology*; D012463:Saliva / Q000378:metabolism*; D012907:Smoking / Q000453:epidemiology*; D013315:Stress, Psychological / Q000378:metabolism; D015813:Substance Abuse Detection / Q000379:methods
chemical_list: D006854:Hydrocortisone