EL5 is involved in root development as an anti-cell death ubiquitin ligase.
Ubiquitin ligase (E3) plays a central role in substrate recognition during ubiquitination, a post-translational modification of proteins. Rice EL5 is an E3 with a RING-H2 finger domain (RFD) and its transcript is upregulated by a chitin elicitor. The EL5-RFD has been intensively studied and demonstrated to exhibit E3 activity. Its three-dimensional structure was determined for the first time in plant E3, and the amino acid residues required for the interaction with the ubiquitin-conjugating enzyme (E2) were identified. Recent analyses revealed that EL5 plays a crucial role as an E3 in the maintenance of cell viability during root development in rice. In this addendum, we report that the EL5-RFD catalyzes polyubiquitination via the Lys48 residue of ubiquitin. We also discuss the possible role of EL5 as an anti-cell death enzyme. We hypothesize that EL5 might be responsible for mediating the degradation of cytotoxic proteins produced in root cells after the actions of phytohormones.
Nishizawa
Yoko
Y
Division of Plant Sciences; National Institute of Agrobiological Sciences; Tsukuba, Japan.
Katoh
Shizue
S
Koiwai
Hanae
H
Katoh
Etsuko
E
FEBS Lett. 2001 Dec 7;509(2):174-6
11741583
Nat Rev Mol Cell Biol. 2003 Jun;4(6):491-7
12778128
Science. 2003 Dec 12;302(5652):1956-60
14671301
Cell. 2000 Jul 7;102(1):67-76
10929714
J Mol Evol. 2006 Apr;62(4):434-45
16557337
Annu Rev Biochem. 1996;65:801-47
8811196
Annu Rev Cell Dev Biol. 2005;21:485-509
16212504
J Biol Chem. 2005 Dec 9;280(49):41015-24
16186120
Plant Mol Biol. 2006 Feb;60(3):343-63
16514559
Plant J. 2007 Jul;51(1):92-104
17559513
Plant Cell Physiol. 2005 Jun;46(6):923-30
15829512
Nat Rev Mol Cell Biol. 2001 Mar;2(3):169-78
11265246
Plant Sci. 2001 Mar;160(4):577-583
11448732
Development. 2003 Apr;130(7):1429-38
12588857
J Biol Chem. 2003 Apr 25;278(17):15341-8
12588869
Plant J. 2002 May;30(4):447-55
12028574
Mol Gen Genet. 1996 Oct 16;252(5):587-96
8914520
Plant Physiol. 2000 Dec;124(4):1718-27
11115888
Plant Physiol. 2002 Jul;129(3):954-6
12114551