[Detection of APC gene promoter methylation in hematological malignant cell lines by nested-methylation specific polymerase chain reaction].
This study was aimed to investigate the efficiency of nested methylation specific polymerase chain reaction (nMS-PCR) for detecting the APC gene promoter methylation and to clarify the roles of methylation in genesis and development of hematologic malignancies, as well as to screen the hematologic malignant cell lines with hypermethylation of APC gene promoter to use as an ideal cell model for exploring the relationship between gen methylation and gene expression. The genome DNA of 10 cell lines modified with bisulfide was amplified and the methylation status of APC gene promoter was detected by using nMS-PCR. The results showed that the methylation of APC gene promoter was detected in Jurkat cells, while could not be detected in CA46, U266, Molt4, K562, HL-60, CEM, AKR, U937 and Raji cell lines. In conclusion, APC gene methylation in hematological malignant cell lines can be accurately detected by nMS-PCP method, which is simple method for detecting methylation status of various hematological malignant cell lines.
Wu
Xue-Mei
XM
Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujiam Province, China.
Shen
Jian-Zhen
JZ
Yu
Ai-Fang
AF
Fan
Li-Ping
LP
Zhou
Hua-Rong
HR
Fu
Hai-Ying
HY
Shen
Song-Fei
SF
Wu
Dan-Sen
DS
D019175:DNA Methylation; D017491:Genes, APC; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic