Real-time PCR and enrichment culture for sensitive detection and enumeration of Escherichia coli.
Rapid enumeration of Escherichia coli strains by quantitative real-time PCR targeting the uidA gene was developed and confirmed for minced beef, tuna and raw oyster. Higher sensitivity (1 CFU/g of E. coli in all three food samples) was obtained by incubating for 7 h in TSB. Colony-directed E. coli specific TaqMan PCR assay could effectively distinguish colonies grown on various selective media within 1.5-h. Inspection of E. coli in food testing laboratories is important, and our rapid E. coli detection strategy will contribute to quality control in food industries.
Takahashi
Hajime
H
Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, 5-7 Konan 4, Minato, Tokyo 108-8477, Japan.
Kimura
Bon
B
Tanaka
Yuichiro
Y
Shinozaki
Junko
J
Suda
Takayuki
T
Fujii
Tateo
T
D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins