title: Detection of dog filariasis in Marajo Island, Brazil by classical and molecular methods.
abstract: Canine filariasis in domestic and wild dogs, foxes, and wolves is caused by several species of filarids. Although these filarial species inhabit different loci in the vertebrate definitive hosts, they generally release microfilariae into the bloodstream. Data about filarial infection in dogs in Brazil, especially on the Marajo Island, is scarce. For this reason, we conducted an analysis of 188 domestic dogs within two Marajo Island municipalities. The overall prevalence of microfilaremic was 32.45%; taken by blood smear and modified Knott's method. No significant difference of positivity between male and female was observed (X(2) Yates's correction = 0.341; p = 0.559). Significant age-infection ratios were detected (X(2) = 32.943; p < 0.0001). A high occult infection was detected (53.84%). PCR of rDNA and phylogenetic tree indicated that the microfilariae and adult worms found in domestic dogs from Marajo Island were Dirofilaria immitis.
authors.affiliation: Laboratório de Biologia Celular e Helmintologia Profa. Dra. Reinalda Marisa Lanfredi, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Adriano P
authors.initials: AP
authors.lastname: Furtado
authors.forename: Eder S
authors.initials: ES
authors.lastname: Do Carmo
authors.forename: Elane G
authors.initials: EG
authors.lastname: Giese
authors.forename: Antonio C R
authors.initials: AC
authors.lastname: Vallinoto
authors.forename: Reinalda M
authors.initials: RM
authors.lastname: Lanfredi
authors.forename: Jeannie N
authors.initials: JN
authors.lastname: Santos
mesh_terms: D000818:Animals; D001483:Base Sequence; D001769:Blood / Q000469:parasitology; D001938:Brazil / Q000453:epidemiology; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004183:Dirofilaria immitis / Q000033:anatomy & histology / Q000235:genetics / Q000302:isolation & purification*; D004283:Dog Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs; D005260:Female; D005368:Filariasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D008853:Microscopy / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal
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