The antifungal activity of Sarcococca saligna ethanol extract and its combination effect with fluconazole against different resistant Aspergillus species.
Microbial resistance is a major drawback in chemotherapy of microbial or fungal infection disease. In this study, the antifungal activity of ethanol extract of a selected plant (Sarcococca saligna) has been investigated against clinical isolates of Aspergillus niger, Aspergillus treus, Aspergillus flavus, and Aspergillus fumigatus. Also, the enhancement of the antifungal activity of fluconazole by this extract was further evaluated against mentioned test strains. Conventional disk diffusion method was used to assay the antifungal activity of S. saligna ethanol extract in the absence and presence of fluconazole. The highest antifungal activity was observed against A. treus. The ethanol extract of S. saligna enhanced the antifungal activity of fluconazole against A. niger and A. treus and A. flavus. At the highest tested contents (4 mg/disk), 1.15-, 0.64-, and 2.47-fold increases in inhibition zone surface area were observed for A. niger, A. treus, and A. flavus, respectively. However, no enhancing effect was observed for this plant extract against Aspergillus fumigates at tested contents (0.5, 1, 2, 3, and 4 mg/disk). In a separate experiment, the general cytotoxicity of the ethanol extract of S. saligna was examined with brine shrimp assay. This plant extract showed low cytotoxicity against Artemia salina (LC(50) = 186 microg/ml).
Mollazadeh Moghaddam
Kamyar
K
Student Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Arfan
Mohammad
M
Rafique
Jamal
J
Rezaee
Sassan
S
Jafari Fesharaki
Parisa
P
Gohari
Ahmad Reza
AR
Shahverdi
Ahmad Reza
AR
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology* / Q000633:toxicity; D001156:Artemia / Q000187:drug effects; D001230:Aspergillus / Q000187:drug effects*; D031234:Buxaceae / Q000737:chemistry*; D052978:Disk Diffusion Antimicrobial Tests; D004347:Drug Interactions; D025141:Drug Resistance, Fungal / Q000187:drug effects*; D000431:Ethanol / Q000737:chemistry*; D015725:Fluconazole / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity
D000935:Antifungal Agents; D010936:Plant Extracts; D000431:Ethanol; D015725:Fluconazole