title: The Drosophila foraging gene mediates adult plasticity and gene-environment interactions in behaviour, metabolites, and gene expression in response to food deprivation.
abstract: Nutrition is known to interact with genotype in human metabolic syndromes, obesity, and diabetes, and also in Drosophila metabolism. Plasticity in metabolic responses, such as changes in body fat or blood sugar in response to changes in dietary alterations, may also be affected by genotype. Here we show that variants of the foraging (for) gene in Drosophila melanogaster affect the response to food deprivation in a large suite of adult phenotypes by measuring gene by environment interactions (GEI) in a suite of food-related traits. for affects body fat, carbohydrates, food-leaving behavior, metabolite, and gene expression levels in response to food deprivation. This results in broad patterns of metabolic, genomic, and behavioral gene by environment interactions (GEI), in part by interaction with the insulin signaling pathway. Our results show that a single gene that varies in nature can have far reaching effects on behavior and metabolism by acting through multiple other genes and pathways.
authors.affiliation: Department of Biology, University of Toronto Mississauga, Ontario, Canada.
authors.forename: Clement F
authors.initials: CF
authors.lastname: Kent
authors.forename: Tim
authors.initials: T
authors.lastname: Daskalchuk
authors.forename: Lisa
authors.initials: L
authors.lastname: Cook
authors.forename: Marla B
authors.initials: MB
authors.lastname: Sokolowski
authors.forename: Ralph J
authors.initials: RJ
authors.lastname: Greenspan
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D017869:Cyclic GMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D017753:Ecosystem; D005223:Fats / Q000378:metabolism; D005508:Food Deprivation; D015870:Gene Expression; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; D005223:Fats; D017869:Cyclic GMP-Dependent Protein Kinases; C528566:for protein, Drosophila
references.citation: Learn Mem. 2008 Oct 02;15(10):792-805
references.pmid: 18832566
references.citation: Nature. 2004 Jul 15;430(6997):317-22
references.pmid: 15220933
references.citation: J Neurochem. 1997 Feb;68(2):443-56
references.pmid: 9003029
references.citation: Genes Dev. 2007 Feb 1;21(3):235-7
references.pmid: 17289913
references.citation: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9440-5
references.pmid: 12883005
references.citation: Int J Biochem Cell Biol. 2009 May;41(5):1006-10
references.pmid: 18992839
references.citation: Nat Cell Biol. 2002 Mar;4(3):251-5
references.pmid: 11862217
references.citation: J Exp Biol. 2003 Jul;206(Pt 14):2507-15
references.pmid: 12796464
references.citation: Development. 2002 Sep;129(17):4103-9
references.pmid: 12163412
references.citation: J Neurosci. 1999 Oct 1;19(19):RC28
references.pmid: 10493773
references.citation: J Exp Biol. 1949 Aug;26(2):150-63, illust
references.pmid: 15395188
references.citation: Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15020-5
references.pmid: 11752451
references.citation: FEBS Lett. 1997 Jun 23;410(1):3-10
references.pmid: 9247112
references.citation: Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5044-6
references.pmid: 8506349
references.citation: Heredity (Edinb). 1999 Dec;83 ( Pt 6):637-43
references.pmid: 10651907
references.citation: Genes Dev. 2003 Aug 15;17(16):2006-20
references.pmid: 12893776
references.citation: Science. 1997 Aug 8;277(5327):834-6
references.pmid: 9242616
references.citation: J Neurosci. 1998 Mar 15;18(6):2254-67
references.pmid: 9482810
references.citation: Ther Drug Monit. 2005 Dec;27(6):747-51
references.pmid: 16404815
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16829-34
references.pmid: 12486227
references.citation: Nat Genet. 2002 Aug;31(4):349-53
references.pmid: 12042820
references.citation: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D354-7
references.pmid: 16381885
references.citation: Learn Mem. 2000 Sep-Oct;7(5):341-52
references.pmid: 11040266
references.citation: Genetics. 1989 Sep;123(1):131-44
references.pmid: 2530131
references.citation: Planta. 2007 Apr;225(5):1085-106
references.pmid: 17077972
references.citation: Physiol Genomics. 2007 Mar 14;29(1):24-34
references.pmid: 17090700
references.citation: J Insect Physiol. 2003 Oct;49(10):933-43
references.pmid: 14511826
references.citation: Genetics. 2005 Apr;169(4):2151-63
references.pmid: 15687281
references.citation: Mol Ecol. 2005 Mar;14(3):813-8
references.pmid: 15723672
references.citation: Integr Comp Biol. 2004 Feb;44(1):28-36
references.pmid: 21680483
references.citation: J Exp Biol. 2007 Oct;210(Pt 20):3547-58
references.pmid: 17921156
references.citation: Curr Biol. 2006 May 23;16(10):958-70
references.pmid: 16713952
references.citation: Dev Cell. 2002 Feb;2(2):239-49
references.pmid: 11832249
references.citation: Genetics. 1989 Aug;122(4):837-45
references.pmid: 2503423
references.citation: J Neurosci. 1996 May 15;16(10):3486-99
references.pmid: 8627381
references.citation: J Exp Biol. 1997 Jun;200(Pt 12):1821-32
references.pmid: 9225453
references.citation: Nat Rev Genet. 2001 May;2(5):383-7
references.pmid: 11331904
references.citation: PLoS One. 2007 Aug 22;2(8):e773
references.pmid: 17712421
references.citation: Dev Cell. 2005 Aug;9(2):271-81
references.pmid: 16054033
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15911-5
references.pmid: 17043223
references.citation: Genome Biol. 2003;4(5):P3
references.pmid: 12734009
references.citation: Cell Metab. 2008 Mar;7(3):249-57
references.pmid: 18316030
references.citation: Nat Genet. 2006 Sep;38(9):1023-31
references.pmid: 16906161
references.citation: Curr Opin Neurobiol. 1997 Dec;7(6):805-11
references.pmid: 9464983
references.citation: Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13051-5
references.pmid: 17640898
references.citation: BMC Dev Biol. 2003 Jul 5;3:5
references.pmid: 12844367
references.citation: Genetics. 1989 Sep;123(1):157-63
references.pmid: 2509284
references.citation: J Biol Chem. 1989 Jun 25;264(18):10738-48
references.pmid: 2732245
references.citation: Proc Biol Sci. 2004 Jun 22;271(1545):1225-34
references.pmid: 15306346
references.citation: Trends Ecol Evol. 1995 May;10(5):212-7
references.pmid: 21237012
references.citation: J Biol. 2003;2(3):20
references.pmid: 12908874
references.citation: Learn Mem. 2004 May-Jun;11(3):303-11
references.pmid: 15169860
references.citation: Cell. 1999 Jun 25;97(7):865-75
references.pmid: 10399915
references.citation: Rev Neurosci. 2005;16(1):23-41
references.pmid: 15810652
references.citation: Cereb Cortex. 2003 Aug;13(8):864-9
references.pmid: 12853373
references.citation: Cell Metab. 2008 Apr;7(4):321-32
references.pmid: 18396138
references.citation: Curr Biol. 1999 Sep 23;9(18):1019-29
references.pmid: 10508611
references.citation: Science. 2001 Apr 6;292(5514):104-6
references.pmid: 11292874
references.citation: Bioessays. 2002 Mar;24(3):267-74
references.pmid: 11891763
references.citation: Nat Rev Genet. 2001 Jan;2(1):11-20
references.pmid: 11253063
references.citation: Genetics. 2008 Jun;179(2):1079-88
references.pmid: 18505870
references.citation: J Insect Physiol. 2008 Sep;54(9):1371-6
references.pmid: 18706419
references.citation: Genes Dev. 2005 Oct 15;19(20):2435-46
references.pmid: 16230533
references.citation: Heredity (Edinb). 2007 Jan;98(1):28-37
references.pmid: 16955112
references.citation: Science. 2008 Apr 25;320(5875):495-7
references.pmid: 18436781
references.citation: Gen Comp Endocrinol. 2003 Jun 1;132(1):10-20
references.pmid: 12765639
references.citation: Nat Rev Genet. 2004 Sep;5(9):681-90
references.pmid: 15372091
references.citation: Learn Mem. 2007 May 03;14(5):342-9
references.pmid: 17522025
references.citation: Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6351-6
references.pmid: 19332792
references.citation: Nature. 2004 Jun 3;429(6991):562-6
references.pmid: 15175753
references.citation: J Insect Physiol. 1970 Oct;16(10):1839-44
references.pmid: 5470528
references.citation: Science. 2003 Oct 10;302(5643):249-55
references.pmid: 12934013
references.citation: J Exp Biol. 2008 Oct;211(Pt 19):3160-6
references.pmid: 18805815
references.citation: Bioinformatics. 2003 Jan 22;19(2):185-93
references.pmid: 12538238
references.citation: Evolution. 1985 May;39(3):505-522
references.pmid: 28561964
references.citation: J Comp Neurol. 2007 Oct 10;504(5):570-82
references.pmid: 17701979
references.citation: Pharmacol Ther. 2002 Feb-Mar;93(2-3):203-15
references.pmid: 12191612
references.citation: Nature. 2007 May 10;447(7141):210-2
references.pmid: 17495926
references.citation: FEBS Lett. 2003 Dec 4;555(2):307-10
references.pmid: 14644433
references.citation: Genome. 1993 Feb;36(1):94-101
references.pmid: 8458574
references.citation: Evolution. 1991 May;45(3):630-648
references.pmid: 28568816
references.citation: Annu Rev Entomol. 2006;51:1-24
references.pmid: 16332201
references.citation: Curr Biol. 2001 Feb 20;11(4):213-21
references.pmid: 11250149