title: Osteopontin, CD44, and NFkappaB expression in gastric adenocarcinoma.
abstract: OBJECTIVE
  Osteopontin (OPN) binds to CD44 and nuclear factor-kappaB (NFkappaB) and OPN mediates tumorigenesis, invasion and metastasis, but the interrelationships between OPN, CD44 and NFkappaB are not fully understood, and especially in gastric carcinogenesis. We examined the expressions of OPN, CD44, and NFkappaB in untreated gastric adenocarcinomas.
  
  
  METHODS
  The materials from 211 cases of gastric adenocarcinoma were immunostained for OPN, CD44 and NFkappaB by using a tissue microarray. The OPN mRNA expression was measured in 10 cases by performing real-time RT-PCR.
  
  
  RESULTS
  The expression of OPN, CD44 and NFkappaB was noted in 61.7%, 11.4% and 26.6% of the adenocarcinoma tissues, respectively. No significant correlation was detected among the expressions of these proteins. The OPN protein expression was negatively correlated with angioinvasion (p<0.05) and patient survival (p<0.05), whereas the CD44 and NFkappaB protein expressions were not correlated with any of the clinicopathological factors we examined. The depth of invasion, lymph node status and perineural invasions were prognostic factors based on the Cox analysis. The OPN mRNA expression showed no significant difference between the adenocarcinoma and the paired normal mucosa on real-time RT-PCR.
  
  
  CONCLUSIONS
  OPN may have a currently undetermined role in gastric carcinogenesis, and CD44 and NFkappaB may have minor roles in gastric adenocarcinoma.
authors.affiliation: Department of Pathology, Inje University Sanggye Paik Hospital, Seoul, Korea.
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Byung-Noe
authors.initials: BN
authors.lastname: Bae
authors.forename: Kyoung Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Eunah
authors.initials: E
authors.lastname: Shin
authors.forename: Kyeongmee
authors.initials: K
authors.lastname: Park
references.citation: J Biol Chem. 2005 Jan 28;280(4):2708-13
references.pmid: 15557322
references.citation: Cancer Res. 2007 Mar 1;67(5):2089-97
references.pmid: 17332338
references.citation: Appl Immunohistochem Mol Morphol. 2004 Mar;12(1):67-70
references.pmid: 15163022
references.citation: J Gastroenterol Hepatol. 2008 Mar;23(3):351-65
references.pmid: 18318820
references.citation: J Mammary Gland Biol Neoplasia. 2001 Oct;6(4):419-29
references.pmid: 12013531
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Biochim Biophys Acta. 2001 Dec 28;1552(2):61-85
references.pmid: 11825687
references.citation: J Biol Chem. 2003 Apr 18;278(16):14487-97
references.pmid: 12473670
references.citation: J Clin Oncol. 2005 May 20;23(15):3526-35
references.pmid: 15908663
references.citation: Oncogene. 2003 Feb 27;22(8):1198-205
references.pmid: 12606946
references.citation: Clin Exp Metastasis. 2004;21(6):515-23
references.pmid: 15679049
references.citation: Clin Cancer Res. 2004 Jan 1;10(1 Pt 1):184-90
references.pmid: 14734468
references.citation: Mol Carcinog. 2005 Aug;43(4):225-36
references.pmid: 15864800
references.citation: Gut. 2007 Jun;56(6):782-9
references.pmid: 17148500
references.citation: Scand J Surg. 2006;95(4):225-31
references.pmid: 17249269
references.citation: J Biol Chem. 2004 Feb 6;279(6):4541-50
references.pmid: 14623895
references.citation: Carcinogenesis. 2005 Apr;26(4):741-51
references.pmid: 15661802
references.citation: J Biol Chem. 2009 Jan 30;284(5):2657-2671
references.pmid: 19047049
references.citation: Genes Dev. 2002 Dec 1;16(23):3074-86
references.pmid: 12464636
references.citation: J Biol Chem. 2001 Nov 30;276(48):44926-35
references.pmid: 11564733
references.citation: J Clin Endocrinol Metab. 2005 Sep;90(9):5270-8
references.pmid: 15998773
references.citation: Mol Cancer. 2007 Jan 16;6:6
references.pmid: 17227585
references.citation: J Oral Pathol Med. 2007 Jan;36(1):30-4
references.pmid: 17181739
references.citation: J Neurooncol. 2008 Feb;86(3):285-96
references.pmid: 17928956