title: Fluoroquinolone resistance in Mycobacterium tuberculosis and mutations in gyrA and gyrB.
abstract: This study evaluated cross-resistance of Mycobacterium tuberculosis strains to ofloxacin, moxifloxacin, and gatifloxacin and investigated the presence of mutations in gyrA and gyrB. Fluoroquinolone susceptibilities were determined for 41 M. tuberculosis strains by the proportion method on 7H11, and MICs were determined by the resazurin microtiter assay. Forty strains shared the same resistance results for the three fluoroquinolones. However, one strain, with an Asn-533 --> Thr mutation in gyrB, was susceptible to ofloxacin but resistant to moxifloxacin and gatifloxacin.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
authors.forename: Andrea
authors.initials: A
authors.lastname: Von Groll
authors.forename: Anandi
authors.initials: A
authors.lastname: Martin
authors.forename: Pontus
authors.initials: P
authors.lastname: Jureen
authors.forename: Sven
authors.initials: S
authors.lastname: Hoffner
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Palomino
authors.forename: Pedro Almeida
authors.initials: PA
authors.lastname: da Silva
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001372:Aza Compounds / Q000494:pharmacology; D027081:DNA Gyrase / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology*; D000077734:Gatifloxacin; D000077266:Moxifloxacin; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D015242:Ofloxacin / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D015242:Ofloxacin; D027081:DNA Gyrase; D000077734:Gatifloxacin; D000077266:Moxifloxacin
references.citation: Clin Pharmacol Ther. 2007 Nov;82(5):595-600
references.pmid: 17898708
references.citation: Antimicrob Agents Chemother. 1998 Aug;42(8):2084-8
references.pmid: 9687411
references.citation: J Clin Microbiol. 2005 Apr;43(4):1612-6
references.pmid: 15814974
references.citation: Chemotherapy. 2006;52(4):190-5
references.pmid: 16714850
references.citation: Southeast Asian J Trop Med Public Health. 2005;36 Suppl 4:228-37
references.pmid: 16438215
references.citation: Antimicrob Agents Chemother. 2002 Apr;46(4):1022-5
references.pmid: 11897584
references.citation: Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):362-6
references.pmid: 8552639
references.citation: Microb Drug Resist. 2006 Spring;12(1):7-11
references.pmid: 16584301
references.citation: Tuber Lung Dis. 1998;79(2):111-8
references.pmid: 10645449
references.citation: Int J Tuberc Lung Dis. 2008 Feb;12(2):128-38
references.pmid: 18230244
references.citation: Int J Tuberc Lung Dis. 2008 Jul;12(7):736-42
references.pmid: 18544197
references.citation: Bull Exp Biol Med. 2008 Jan;145(1):108-13
references.pmid: 19024017
references.citation: Clin Microbiol Rev. 2006 Apr;19(2):382-402
references.pmid: 16614254
references.citation: J Infect Dis. 1996 Nov;174(5):1127-30
references.pmid: 8896523
references.citation: Int J Infect Dis. 2002 Mar;6(1):48-51
references.pmid: 12044302
references.citation: J Chemother. 2005 Apr;17(2):169-73
references.pmid: 15920901
references.citation: J Antimicrob Chemother. 1999 Sep;44(3):393-5
references.pmid: 10511409
references.citation: Am J Respir Crit Care Med. 2004 Nov 15;170(10):1131-4
references.pmid: 15306535
references.citation: Antimicrob Agents Chemother. 1995 Aug;39(8):1700-3
references.pmid: 7486904
references.citation: Antimicrob Agents Chemother. 2008 Mar;52(3):852-7
references.pmid: 18070980
references.citation: Antimicrob Agents Chemother. 2000 Oct;44(10):2600-3
references.pmid: 10991830
references.citation: Int J Antimicrob Agents. 2008 Feb;31(2):115-21
references.pmid: 18164184
references.citation: Antimicrob Agents Chemother. 2004 Aug;48(8):3175-8
references.pmid: 15273144
references.citation: Antimicrob Agents Chemother. 1994 Apr;38(4):773-80
references.pmid: 8031045