title: Expressive proteomics profile changes of injured human brain cortex due to acute brain trauma.
abstract: OBJECTIVE
  To find the expressive proteomics changes in damaged human brain cortex after traumatic brain injury (TBI).
  
  
  METHODS
  By rapid high-throughput and precise proteomic techniques, the traumatic injured human frontal cortexes were compared with non-trauma controls.
  
  
  RESULTS
  On 2-DE PAGE, 138 protein spots were found significantly different on expressive level of quantitative mature. Most of these proteins expressed in a fluctuant fashion within 18 hours after trauma, with mean levels lower than control. Eighty-two protein spots were identified by MALDI-MS TOF, which were products of 71 proteins and could be grouped into 10 categories based on possible functions: cytoskeleton (n = 10), metabolism (n = 13), electron transport (n = 8), signalling transduction (n = 4), stress response (n = 6), protein synthesis and turnover (n = 8), transporter (n = 5), cell cycle (n = 1), other (n = 8) and unknown (n = 9).
  
  
  CONCLUSIONS
  After traumatic brain injury, there are significant proteins expressing changes in damaged brain tissue. These proteins may play a critical role in TBI. Although some of these proteins functions are not fully understood, they may become novel biomarkers and novel therapy targets in the future.
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, PR China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Yang
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Guoliang
authors.initials: G
authors.lastname: Hong
mesh_terms: D000328:Adult; D000368:Aged; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012016:Reference Values; D055815:Young Adult
chemical_list: D020543:Proteome