title: A new setup for in vivo fluorescence imaging of photosynthetic activity.
abstract: Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or petri dishes. In the choice of CCD, we have opted for a 12-bits high frame rate [150 fps (frames per second)] at the expense of definition (640 x 480 pixels). Although the choice of digital camera is always a compromise between these two related features, we have designed a flexible system allowing the fast sampling of images (down to 100 micros) with a maximum spatial resolution. This image readout system, synchronized with actinic light and saturating pulses, allows a precise determination of F(0) and F(M), which is required to monitor PSII activity. This new imaging system, together with image processing techniques, is useful to investigate the heterogeneity of photosynthetic activity within leaves or to screen large numbers of unicellular algal mutant colonies to identify those with subtle changes in photosynthetic electron flow.
authors.affiliation: Institut de Biologie Physico-Chimique, UMR 7141, CNRS et Université Pierre et Marie Curie (Paris VI), Paris, France.
authors.forename: Xenie
authors.initials: X
authors.lastname: Johnson
authors.forename: Guillaume
authors.initials: G
authors.lastname: Vandystadt
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bujaldon
authors.forename: Francis-André
authors.initials: FA
authors.lastname: Wollman
authors.forename: Rémi
authors.initials: R
authors.lastname: Dubois
authors.forename: Pierre
authors.initials: P
authors.lastname: Roussel
authors.forename: Jean
authors.initials: J
authors.lastname: Alric
authors.forename: Daniel
authors.initials: D
authors.lastname: Béal
mesh_terms: D015153:Blotting, Western; D002696:Chlamydomonas / Q000502:physiology; D045346:Cytochrome b6f Complex / Q000378:metabolism; D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D010084:Oxidation-Reduction; D010788:Photosynthesis / Q000502:physiology*; D013997:Time Factors
chemical_list: D045346:Cytochrome b6f Complex
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