title: Comparative studies on the analysis of glycoproteins and lipopolysaccharides by the gel-based microchip and SDS-PAGE.
abstract: In order to determine time efficiency between the gel-based microchip (LabChip) and traditional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), glycoproteins and lipopolysaccharides were analyzed in this study. After 90 min of gel electrophoresis, glycoproteins (bovine serum albumin, lysozyme, ovalbumin, and apo-transferrin) and fluorescent lipopolysaccharides (LPS-O and LPS-S) under reducing conditions could be analyzed by SDS-PAGE, and it would take (including imaging and analyzing) more than 3 h. The same sample could also be assayed on a Bioanalyzer in combination with the LabChip, and it would only need 30 min from start to finish. The assay software automatically calculated the size and concentration of each separated peak and displayed the results in real time, thus eliminating time-consuming procedures such as imaging and analyzing. Compared to the traditional reducing SDS-PAGE, LabChip has a faster turnaround time.
authors.affiliation: Institute of Biological Chemistry and the Genomics Research Center, Academia Sinica, Nankang, Taipei 115, Taiwan.
authors.forename: Jung-Feng
authors.initials: JF
authors.lastname: Hsieh
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
references.citation: Analyst. 2005 Apr;130(4):528-33
references.pmid: 15776163
references.citation: Anal Chem. 2001 Oct 15;73(20):4994-9
references.pmid: 11681478
references.citation: Electrophoresis. 2002 Oct;23(20):3623-9
references.pmid: 12412133
references.citation: Annu Rev Biophys Biomol Struct. 2000;29:155-81
references.pmid: 10940246
references.citation: Electrophoresis. 2000 Jan;21(1):3-11
references.pmid: 10634466
references.citation: J Agric Food Chem. 2004 Jun 2;52(11):3617-25
references.pmid: 15161240
references.citation: Carbohydr Res. 2001 Jan 15;330(1):131-9
references.pmid: 11217956
references.citation: Ann Occup Hyg. 2004 Jan;48(1):65-73
references.pmid: 14718347
references.citation: Anal Chem. 2001 Mar 15;73(6):1207-12
references.pmid: 11305653
references.citation: Proteomics. 2001 Jul;1(7):841-55
references.pmid: 11503209
references.citation: Electrophoresis. 2004 Nov;25(21-22):3890-6
references.pmid: 15565674
references.citation: Eur J Biochem. 1983 Jul 1;133(3):685-8
references.pmid: 6190650
references.citation: Electrophoresis. 1988 Jun;9(6):255-62
references.pmid: 2466658
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Sep 25;795(1):1-8
references.pmid: 12957163
references.citation: Methods Enzymol. 1990;182:587-601
references.pmid: 2314259
references.citation: Electrophoresis. 2003 Feb;24(4):588-98
references.pmid: 12601726
references.citation: Graefes Arch Clin Exp Ophthalmol. 2002 Nov;240(11):900-5
references.pmid: 12486511
references.citation: Proteomics. 2005 Sep;5(14):3722-30
references.pmid: 16127733
references.citation: Nucleic Acids Res. 2006 Feb 07;34(3):e17
references.pmid: 16464820