Genes of cathepsin L-like proteases in Trypanosoma rangeli isolates: markers for diagnosis, genotyping and phylogenetic relationships.
We have sequenced genes encoding cathepsin L-like (CatL-like) cysteine proteases from isolates of Trypanosoma rangeli from humans, wild mammals and Rhodnius species of Central and South America. Phylogenetic trees of sequences encoding mature CatL-like enzymes of T. rangeli and homologous genes from other trypanosomes, Leishmania spp. and bodonids positioned sequences of T. rangeli (rangelipain) closest to T. cruzi (cruzipain). Phylogenetic tree of kinetoplastids based on sequences of CatL-like was totally congruent with those derived from SSU rRNA and gGAPDH genes. Analysis of sequences from the CatL-like catalytic domains of 17 isolates representative of the overall phylogenetic diversity and geographical range of T. rangeli supported all the lineages (A-D) previously defined using ribosomal and spliced leader genes. Comparison of the proteolytic activities of T. rangeli isolates revealed heterogeneous banding profiles of cysteine proteases in gelatin gels, with differences even among isolates of the same lineage. CatL-like sequences proved to be excellent targets for diagnosis and genotyping of T. rangeli by PCR. Data from CatL-like encoding genes agreed with results from previous studies of kDNA markers, and ribosomal and spliced leader genes, thereby corroborating clonal evolution, independent transmission cycles and the divergence of T. rangeli lineages associated with sympatric species of Rhodnius.
Ortiz
P A
PA
Department of Parasitology, University of São Paulo (USP), São Paulo, SP, 05508-900, Brazil.
Maia da Silva
F
F
Cortez
A P
AP
Lima
L
L
Campaner
M
M
Pral
E M F
EM
Alfieri
S C
SC
Teixeira
M M G
MM
D000818:Animals; D001483:Base Sequence; D056668:Cathepsin L / Q000235:genetics* / Q000302:isolation & purification; D002489:Central America; D016000:Cluster Analysis; D004586:Electrophoresis / Q000379:methods; D005838:Genotype; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013020:South America; D014345:Trypanosoma / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000175:diagnosis* / Q000469:parasitology*
D015800:Protozoan Proteins; D056668:Cathepsin L