title: Interleukin-27 polymorphisms are associated with inflammatory bowel diseases in a Korean population.
abstract: OBJECTIVE
  The cytokine interleukin (IL)-27 is composed of two subunits, Epstein-Barr virus-induced gene 3 (EBI3) and p28, and IL-27 is a novel IL-12 family member that mediates between the innate and adaptive immune systems. We previously identified four polymorphisms in the human IL-27 gene and we suggested that the polymorphism of IL-27 is associated with the susceptibility to asthma. IL-27 transcripts are significantly elevated in active Crohn's disease (CD) but not in ulcerative colitis (UC). To determine whether these IL-27 single nucleotide polymorphisms are associated with the susceptibility to inflammatory bowel disease (IBD), the genotype and allelic frequencies of the IL-27 polymorphisms were analyzed between the IBD patients and the healthy controls.
  
  
  METHODS
  Genotype analysis of the IL-27 gene was performed by the single-base extension (SBE) method. The haplotype frequencies of IL-27 for multiple loci were estimated using the expectation maximization (EM) algorithm.
  
  
  RESULTS
  The genotype frequencies of the g.-964A > G polymorphism in the IBD patients were significantly different from those of the healthy control group (P = 0.001). In both the UC and CD patients, the genotype frequencies of the g.-964A > G polymorphism were also significantly different from the frequencies of the healthy control group (P = 0.009). The frequencies of the AGT and GGT haplotypes were significantly different between the healthy control group and the IBD patient group (P = 0.00004 and 0.021, respectively).
  
  
  CONCLUSIONS
  Our results suggest that the g.-964A > G polymorphism of the IL-27 gene located on the IBD1 locus might be associated with the susceptibility to IBD.
authors.affiliation: Genome Research Center for Immune Disorders, Wonkwang University School of Medicine, Iksan, Chonbuk 570-749, South Korea.
authors.forename: Chun-Shi
authors.initials: CS
authors.lastname: Li
authors.forename: Qinggao
authors.initials: Q
authors.lastname: Zhang
authors.forename: Kwang-Jae
authors.initials: KJ
authors.lastname: Lee
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Cho
authors.forename: Kee-Myung
authors.initials: KM
authors.lastname: Lee
authors.forename: Ki-Baik
authors.initials: KB
authors.lastname: Hahm
authors.forename: Suck-Chei
authors.initials: SC
authors.lastname: Choi
authors.forename: Ki-Jung
authors.initials: KJ
authors.lastname: Yun
authors.forename: Hun-Taeg
authors.initials: HT
authors.lastname: Chung
authors.forename: Soo-Cheon
authors.initials: SC
authors.lastname: Chae
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000208:ethnology / Q000235:genetics* / Q000276:immunology; D003424:Crohn Disease / Q000208:ethnology / Q000235:genetics* / Q000276:immunology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D016015:Logistic Models; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D056910:Republic of Korea / Q000453:epidemiology; D018570:Risk Assessment; D012307:Risk Factors
chemical_list: D007378:Interleukins; C464565:MYDGF protein, human