title: Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase.
abstract: Natural products have the potential to be developed into new drugs for the treatment of various diseases. The aim of the present study was to screen the antioxidant activities of some common edible fruits, garden plants and medicinal plants indigenous to Taiwan. This was performed by assessing the activities of lipoxygenase, xanthine oxidase and tyrosinase following incubation with extracts from these plants. A further aim was to use HPLC-DAD and tyrosinase to chromatographically identify the antioxidative constituents obtained from an extract exhibiting strong antioxidative properties. The acetone extracts of 27 cultivated plant species from Taiwan were tested for antioxidant activities towards xanthine oxidase, tyrosinase and lipoxygenase using spectrophotometric assays. Koelreuteria henryi, Prunus campanulata, and Rhodiola rosea showed the highest xanthine oxidase inhibitory activities. Camellia sinensis, Rhodiola rosea, and Koelreuteria henryi exhibited good tyrosinase inhibitory activities and potent anti-lipoxygenase activities. As Koelreuteria henryi had notable significant inhibitory activities towards xanthine oxidase, tyrosinase, and lipoxygenase, it was further tested with tyrosinase and HPLC-DAD. The results from this part of the study revealed that the more powerful the antioxidant capability of the extracted component, the greater the decrease in peak height obtained after reacting with tyrosinase. Additional studies are warranted to further characterize the compounds responsible for the antioxidant properties of the examined extracts.
authors.affiliation: College of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 11031,Taiwan. chinhui@mail.ypu.edu.tw
authors.forename: Chin-Hui
authors.initials: CH
authors.lastname: Chen
authors.forename: Hsiu-Chen
authors.initials: HC
authors.lastname: Chan
authors.forename: Yi-Tsu
authors.initials: YT
authors.lastname: Chu
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Ho
authors.forename: Pi-Yu
authors.initials: PY
authors.lastname: Chen
authors.forename: Tzong-Huei
authors.initials: TH
authors.lastname: Lee
authors.forename: Ching-Kuo
authors.initials: CK
authors.lastname: Lee
mesh_terms: D027901:Acanthaceae / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008084:Lipoxygenase / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D027861:Prunus / Q000737:chemistry; D029771:Rhodiola / Q000737:chemistry; D013624:Taiwan; D014969:Xanthine Oxidase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D008084:Lipoxygenase; D014442:Monophenol Monooxygenase; D014969:Xanthine Oxidase
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