title: In vivo detection of lamellocytes in Drosophila melanogaster.
abstract: Drosophila has recently become a powerful model organism for studies of innate immunity. The cellular elements of innate immunity in Drosophila, the hemocytes, have been characterized by morphological criteria, molecular markers, and cell-type-specific immunological markers. Here we suggest that an MiET1 GFP-reporter element insertion in the untranslated region of a gene (l1-atilla) - expressed in a subset of hemocytes, the lamellocytes - allows in vivo investigations of lamellocyte differentiation and facilitates genetic screens.
authors.forename: Viktor
authors.initials: V
authors.lastname: Honti
authors.forename: Eva
authors.initials: E
authors.lastname: Kurucz
authors.forename: Gábor
authors.initials: G
authors.lastname: Csordás
authors.forename: Barbara
authors.initials: B
authors.lastname: Laurinyecz
authors.forename: Róbert
authors.initials: R
authors.lastname: Márkus
authors.forename: István
authors.initials: I
authors.lastname: Andó
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004251:DNA Transposable Elements / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional
chemical_list: D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins