Microwave processing and ethanol-based fixation in forensic pathology: an addendum of further scanning electron microscope observations.
Scanning electron microscope microscopy on samples of tissue fixed with alcohol-based fixative and processed using a microwave device confirmed the validity of the fixation procedure. The details are clearer with respect to those obtainable with formalin fixatives. It was interesting to work on sections prepared for normal histologic processing because the metallization indispensable for scanning electron microscope occurred without difficulty. All together, the procedure seems to be very flexible and adapted to the complexity of forensic investigation, above all, when the tissue is altered by autolytic phenomena.
Balbi
Tiziana
T
Institute of Surgical Pathology, S. Orsola-Malpighi Hospital, Bologna, Italy.
Cicognani
Alberto
A
Esposti
Paola Degli
PD
Pierini
Giovanni
G
D004817:Epidermis / Q000648:ultrastructure; D000431:Ethanol; D005404:Fixatives; D049429:Forensic Pathology / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008168:Lung / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D018482:Muscle, Skeletal / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure; D013048:Specimen Handling / Q000379:methods*
D005404:Fixatives; D000431:Ethanol