title: Desmoglein 3 is overexpressed in inverted papilloma and squamous cell carcinoma of sinonasal cavity.
abstract: OBJECTIVE
  We sought to investigate the role of desmoglein 3 in pathogenesis of sinonasal inverted papilloma (IP) and its malignant transformation.
  
  
  METHODS
  Fifteen subjects with sinonasal IP and 15 subjects of normal sphenoid sinus mucosa were enrolled. Each specimen was divided into two portions: one for mRNA expression analysis by real-time polymerase chain reaction, and the other for detection of targeted proteins by immunohistochemistry analysis. In addition, another 10 cases of IP with squamous cell carcinoma (SCC) were added for immunohistochemistry analysis.
  
  
  RESULTS
  The mRNA expression level of desmoglein 3 was significantly higher in IP tissues than in the normal sinus mucosa (P < .001). In immunohistochemistry study, desmoglein 3 was detected in plasma membrane areas of IP and IP with SCC tissues, but no obvious expression was found in normal sinus mucosa (total score; both P < .001). Positive desmoglein 3 staining was strongly present in nearly all malignant transformation areas of IP with SCC cases (90%), but only in scattered areas of some cases of IP (53%) (total score; P < .001).
  
  
  CONCLUSIONS
  Desmoglein 3 was overexpressed in IP and IP with SCC, and the overexpression was correlated with malignant transformation of IP. It may provide valuable insight into the pathobiology of this disease, and can potentially provide a venue to predict malignant transformation in sinonasal IP.
authors.affiliation: Department of Otolaryngoloy-Head and Neck Surgery, Chang Gung Memorial Hospital and Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Chi-Che
authors.initials: CC
authors.lastname: Huang
authors.forename: Ta-Jen
authors.initials: TJ
authors.lastname: Lee
authors.forename: Po-Hung
authors.initials: PH
authors.lastname: Chang
authors.forename: Yun-Shien
authors.initials: YS
authors.lastname: Lee
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Chuang
authors.forename: You-Jia
authors.initials: YJ
authors.lastname: Jhang
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Chen
authors.forename: Chiang-Wen
authors.initials: CW
authors.lastname: Chen
authors.forename: Chi-Neu
authors.initials: CN
authors.lastname: Tsai
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D051184:Desmoglein 3 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009669:Nose Neoplasms / Q000378:metabolism*; D018308:Papilloma, Inverted / Q000378:metabolism*; D010255:Paranasal Sinus Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051184:Desmoglein 3; D012333:RNA, Messenger