Site-specific insertion of IS492 in Pseudoalteromonas atlantica.
Reversible insertion of IS492 at a site within epsG on the Pseudoalteromonas atlantica chromosome controls peripheral extracellular polysaccharide production and biofilm formation by P. atlantica. High-frequency precise excision of IS492 from epsG requires 5 and 7 bp of flanking DNA, suggesting that IS492 transposition involves a site-specific recombination mechanism. The site specificity of IS492 insertion was examined in P. atlantica and shown to be specific for a 7-bp target, 5'-CTTGTTA-3'. Characterization of numerous insertion events at the target site in epsG indicated that insertion is also orientation specific. The frequency of IS492 insertion at the epsG target site (2.7 x 10(-7)/cell/generation), determined by quantitative PCR, is 4 to 5 orders of magnitude lower than the frequency of IS492 precise excision from the same site. Comparison of insertion sites for IS492 and the highly related ISPtu2 from Pseudoalteromonas tunicata suggests DNA sequence and/or structural features that may contribute to site recognition and recombination by the transposase of IS492.
Higgins
Brian P
BP
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
Popkowski
Adam C
AC
Caruana
Peter R
PR
Karls
Anna C
AC
D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D031161:Pseudoalteromonas / Q000235:genetics* / Q000378:metabolism*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial
Trends Ecol Evol. 2000 Mar;15(3):95-99
10675923
J Bacteriol. 1999 Feb;181(3):1021-4
9922269
Nat Rev Mol Cell Biol. 2001 Nov;2(11):806-14
11715047
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15
12824337
J Bacteriol. 1994 Jul;176(13):4160-4
8021196
Curr Opin Microbiol. 2006 Oct;9(5):526-31
16935554
Nucleic Acids Res. 1987 Sep 11;15(17):7053-65
2821490
Genome Res. 1996 Oct;6(10):986-94
8908518
Microbiol Mol Biol Rev. 1998 Sep;62(3):725-74
9729608
J Bacteriol. 1999 Aug;181(16):4937-48
10438765
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3923-7
16593937
Bioinformatics. 2006 Dec 15;22(24):3099-100
17095514
J Bacteriol. 2005 May;187(10):3431-7
15866929
Mol Genet Genomics. 2001 Aug;265(6):1004-10
11523772
J Bacteriol. 1989 Mar;171(3):1763-6
2537827
Curr Opin Struct Biol. 1996 Feb;6(1):76-83
8696976
Mol Microbiol. 1989 Oct;3(10):1307-18
2575701
J Bacteriol. 2006 Jan;188(1):37-44
16352819
Nat Rev Mol Cell Biol. 2003 Nov;4(11):865-77
14682279
J Bacteriol. 2003 Aug;185(16):4891-900
12897009
Cell. 2008 Jan 25;132(2):208-20
18243097
J Bacteriol. 1993 Oct;175(19):6186-93
8104927
J Bacteriol. 2003 Nov;185(21):6371-84
14563872
J Bacteriol. 2008 Jul;190(14):4959-70
18487340
Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1901-6
17264213
Annu Rev Biochem. 2006;75:567-605
16756503
Microbiology (Reading). 1999 Mar;145 ( Pt 3):561-568
10217489
Genome Biol. 2007;8(2):R22
17313685
Mol Microbiol. 2001 Feb;39(3):641-51
11169105
PLoS One. 2008 Sep 24;3(9):e3252
18813346
Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7393-8
10840058
J Bacteriol. 1989 Jan;171(1):602-5
2914859
Nucleic Acids Res. 1989 Nov 25;17(22):9063-73
2555783
FEMS Microbiol Lett. 2009 Jan;290(2):182-7
19025573
Res Microbiol. 2004 Jun;155(5):387-98
15207871