title: The plant NADPH oxidase RBOHD mediates rapid systemic signaling in response to diverse stimuli.
abstract: Cell-to-cell communication and long-distance signaling play a key role in the response of plants to pests, mechanical wounding, and extreme environmental conditions. Here, we report on a rapid systemic signal in Arabidopsis thaliana that traveled at a rate of 8.4 centimeters per minute and was dependent on the respiratory burst oxidase homolog D (RbohD) gene. Signal propagation was accompanied by the accumulation of reactive oxygen species (ROS) in the extracellular spaces between cells and was inhibited by the suppression of ROS accumulation at locations distant from the initiation site. The rapid systemic signal was triggered by wounding, heat, cold, high-intensity light, and salinity stresses. Our results reveal the profound role that ROS play in mediating rapid, long-distance, cell-to-cell propagating signals in plants.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 89557, USA.
authors.forename: Gad
authors.initials: G
authors.lastname: Miller
authors.forename: Karen
authors.initials: K
authors.lastname: Schlauch
authors.forename: Rachel
authors.initials: R
authors.lastname: Tam
authors.forename: Diego
authors.initials: D
authors.lastname: Cortes
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Torres
authors.forename: Vladimir
authors.initials: V
authors.lastname: Shulaev
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Dangl
authors.forename: Ron
authors.initials: R
authors.lastname: Mittler
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000469:parasitology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions; D006358:Hot Temperature; D008027:Light; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D012965:Sodium Chloride; D008156:Luciferases; D019255:NADPH Oxidases