Relationship between expression of voltage-dependent anion channel (VDAC) isoforms and type of hexokinase binding sites on brain mitochondria.
Voltage-dependent anion channels (VDAC) are pore-forming proteins found in the outer mitochondrial membrane of eukaryotes. VDACs are known to play an essential role in cellular metabolism and in early stages of apoptosis. In mammals, three VDAC isoforms have been identified. A proteomic approach was exploited to study the expression of VDAC isoforms in rat, bovine, and chicken brain mitochondria. Given the importance of mitochondrially bound hexokinase in regulation of aerobic glycolysis in brain, we studied the possibility that differences in the relative expression of VDAC isoforms may be a factor in determining the species-dependent ratio of type A/type B hexokinase binding sites on brain mitochondria. The spots were characterized, and the signal intensities among spots were compared. VDAC1 was the most abundantly expressed of the three isoforms. Moreover the expression of VDAC1 plus VDAC2 was significantly higher in bovine than in rat brain. Chicken brain mitochondria showed the highest VDAC1 expression and the lowest of VDAC2. Bovine brain mitochondria had the highest VDAC2 levels. We concluded that the nature of hexokinase binding site is not determined by the expression of a single VDAC isoform.
Poleti
Mirele Daiana
MD
Laboratory of Neuroscience and Proteomics, School of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte 225, 13635-900, Pirassununga, SP, Brazil.
Tesch
Andrea Cristina
AC
Crepaldi
Carla Rossini
CR
Souza
Gustavo Henrique Martins Ferreira
GH
Eberlin
Marcos Nogueira
MN
de Cerqueira César
Marcelo
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional; D006593:Hexokinase / Q000378:metabolism*; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics / Q000378:metabolism*; D050996:Voltage-Dependent Anion Channel 2 / Q000235:genetics / Q000378:metabolism*
D024101:Mitochondrial Proteins; D020033:Protein Isoforms; D050996:Voltage-Dependent Anion Channel 2; D050995:Voltage-Dependent Anion Channel 1; D006593:Hexokinase
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