Loss of calmodulin binding to Bax inhibitor-1 affects Pseudomonas-mediated hypersensitive response-associated cell death in Arabidopsis thaliana.
Bax inhibitor-1 (BI-1) is a cell death suppressor protein conserved across a variety of organisms. The Arabidopsis atbi1-1 plant is a mutant in which the C-terminal 6 amino acids of the expressed BI-1 protein have been replaced by T-DNA insertion. This mutant BI-1 protein (AtBI-CM) produced in Escherichia coli can no longer bind to calmodulin. A promoter-reporter assay demonstrated compartmentalized expression of BI-1 during hypersensitive response, introduced by the inoculation of Pseudomonas syringae possessing the avrRTP2 gene, Pst(avrRPT2). In addition, both BI-1 knockdown plants and atbi1-1 showed increased sensitivity to Pst(avrRPT2)-induced cell death. The results indicated that the loss of calmodulin binding reduces the cell death suppressor activity of BI-1 in planta.
Kawai-Yamada
Maki
M
Department of Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570; Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; Institute for Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570. Electronic address: mkawai@mail.saitama-u.ac.jp.
Hori
Zenta
Z
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
Ogawa
Taro
T
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
Ihara-Ohori
Yuri
Y
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
Tamura
Katsunori
K
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
Nagano
Minoru
M
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
Ishikawa
Toshiki
T
Department of Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570; Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan.
Uchimiya
Hirofumi
H
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032; Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003.
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000033:anatomy & histology / Q000382:microbiology* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011485:Protein Binding; D044224:Pseudomonas syringae / Q000502:physiology*
C491862:ATBI-1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D002147:Calmodulin; D008565:Membrane Proteins
Plant J. 2009 Apr;58(1):122-34
19054355
Mol Cell. 2009 Mar 27;33(6):679-91
19328063
Plant J. 2003 Feb;33(3):425-34
12581301
Mol Cell. 2004 Aug 13;15(3):355-66
15304216
J Biol Chem. 2008 Feb 8;283(6):3200-3210
18039663
Plant J. 2000 Feb;21(4):393-9
10758491
FEBS Lett. 2002 Dec 4;532(1-2):111-4
12459473
Plant Mol Biol. 2000 Oct;44(3):321-34
11199391
Plant J. 2006 Mar;45(6):884-94
16507080
Plant Physiol. 2005 Jun;138(2):1009-17
15908609
FEBS Lett. 2007 Oct 2;581(24):4627-32
17825821
J Biol Chem. 1999 Oct 29;274(44):31727-33
10531384
Nature. 2001 Jun 14;411(6839):848-53
11459068
Cell. 2003 Feb 7;112(3):379-89
12581527
New Phytol. 2003 Sep;159(3):585-598
33873603
Trends Plant Sci. 2005 Aug;10(8):383-9
16023399
New Phytol. 1998 Feb;138(2):251-263
33863084
FEBS Lett. 1999 Dec 31;464(3):143-7
10618494
Mol Cell. 1998 Feb;1(3):337-46
9660918
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5555-60
12704231
PLoS One. 2007 Aug 08;2(8):e718
17684564
Trends Plant Sci. 2002 Jun;7(6):251-6
12049921
Plant Physiol. 2007 Feb;143(2):650-60
17142482
Arch Biochem Biophys. 1996 Mar 01;327(1):53-60
8615696
Mol Plant Microbe Interact. 2000 Mar;13(3):277-86
10707353
Plant Physiol. 2000 Feb;122(2):447-52
10677437
Plant Cell. 1991 Jan;3(1):61-72
1824335
Plant Cell. 2004 Jan;16(1):21-32
14671021
Apoptosis. 2004 May;9(3):299-307
15258461
Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12295-300
11593047
FEBS Lett. 2000 Apr 14;471(2-3):133-6
10767408