[Mapping of diagnostically important antigenic regions of major outer membrane protein (MOMP) from Chlamydia trachomatis using recombinant proteins with overlapping structures].
Amino acid sequence of the Chlamydia trachomatis major outer membrane protein (MOMP) was modeled using a series of the recombinant proteins containing six 100 aa fragments of MOMP overlapped in 30 aa. Testing of recombinant antigens in EIA showed that proteins containing MOMP fragments comprising 191-286 and 191-354 aa regions had the greatest activity in the reaction with anti- C. trachomatis positive serum samples. The data obtained allows the conclusion about the possibility of the use of presented recombinant proteins for development of diagnostic test for anti- C. trachomatis antibodies detection to be made.
Savel'eva
N V
NV
Zagriadskaia
Iu E
IuE
Klimashevskaia
S V
SV
Puzyrev
V F
VF
Burkov
A N
AN
Obriadina
A P
AP
Ulanova
T I
TI
D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D018604:Epitope Mapping; D016133:Polymerase Chain Reaction; D018272:Porins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D011994:Recombinant Proteins; C071677:omp1 protein, Chlamydia trachomatis