Proteomic profiling of acute cardiac allograft rejection.
BACKGROUND
Proteome analysis has emerged as a valuable tool for the study of large-scale protein expression profiles. Here, we applied this novel technology to identify specific biomarkers for acute cardiac allograft rejection.


METHODS
Hearts of C57BL/10 mice were placed in fully major histocompatibility complex-mismatched C3H/He recipients. Syngeneic transplants served as controls. Intragraft protein expression analysis was performed using fluorescence two-dimensional difference gel electrophoresis on day 6 posttransplant. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. In addition, expression of selected proteins was confirmed by Western blot analysis and by immunohistochemistry.


RESULTS
Two-dimensional difference gel electrophoresis enabled detection of 1541 protein spots. For 95 protein spots, the expression level during acute rejection differed by more than 1.5-fold from that observed in syngeneic grafts. Spots with significant differential regulation identified by tandem mass spectrometry were derived from peroxiredoxin 6, pyruvate kinase isozyme M2, coronin 1A, protein disulfide isomerase A3 precursor, and aconitate hydratase.


CONCLUSIONS
These identified proteins may constitute novel biomarkers of acute cardiac allograft rejection and might hold great potential as surrogate markers for monitoring in vivo alloimmune response.
Kienzl
Katrin
K
Department of Visceral, Transplant and Thoracic Surgery, Center of Operative Medicine, Innsbruck Medical University, Innsbruck, Austria.
Sarg
Bettina
B
Golderer
Georg
G
Obrist
Peter
P
Werner
Ernst R
ER
Werner-Felmayer
Gabriele
G
Lindner
Herbert
H
Maglione
Manuel
M
Schneeberger
Stefan
S
Margreiter
Raimund
R
Brandacher
Gerald
G
D000208:Acute Disease; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006084:Graft Rejection / Q000209:etiology / Q000378:metabolism*; D016027:Heart Transplantation / Q000009:adverse effects* / Q000276:immunology / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D054465:Peroxiredoxin VI / Q000378:metabolism; D040081:Protein Array Analysis; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D011770:Pyruvate Kinase / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D015415:Biomarkers; D020543:Proteome; D054465:Peroxiredoxin VI; C515983:Prdx6 protein, mouse; D011770:Pyruvate Kinase