Nano-flares for mRNA regulation and detection.
We build off the previously described concept of a nanoflare to develop an oligonucleotide gold nanoparticle conjugate that is capable of both detecting and regulating intracellular levels of mRNA. We characterize the binding rate and specificity of these materials using survivin, a gene associated with the diagnosis and treatment of cancer, as a target. The nanoconjugate enters cells and binds mRNA, thereby decreasing the relative abundance of mRNA in a dose- and sequence-dependent manner, resulting in a fluorescent response. This represents the first demonstration of a single material capable of both mRNA regulation and detection. Further, we investigate the intracellular biochemistry of the nanoconjugate, elucidating its mechanism of gene regulation. This work is important to the study of biologically active nanomaterials such as the nanoflare and is a first step toward the development of an mRNA responsive "theranostic".
Prigodich
Andrew E
AE
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
Seferos
Dwight S
DS
Massich
Matthew D
MD
Giljohann
David A
DA
Lane
Brandon C
BC
Mirkin
Chad A
CA
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry; D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051017:Apoptosis Regulatory Proteins; D012333:RNA, Messenger
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