title: The complete mitochondrial genomes of two species from Sinocyclocheilus (Cypriniformes: Cyprinidae) and a phylogenetic analysis within Cyprininae.
abstract: We determined the complete mitochondrial DNA sequences for two species of surface- and cave-dwelling-cyprinid fishes, Sinocyclocheilus grahami and S. altishoulderus. Sequence comparison of 13 protein-coding genes shows that the mutation pattern of each single gene is quite similar to those of other vertebrate animal species. Analysis of the ratios of Ka/Ks at these loci between Sinocyclocheilus and two other cyprinid species (Cyprinus carpio and Procypris rabaudi) show that Ka/Ks ratios are differed, consistent with purifying selection and variation in functional constraint among genes. Bayesian analysis and maximum likelihood analysis of the concatenated mitochondrial protein sequences for 14 cyprinid taxa support the monophyly of the family Cyprininae, and further confirm the monophyly of the genus Sinocyclocheilus. The two Sinocyclocheilus species fall within the Cyprinion-Onychostoma lineage, including Cyprinus, Carassius, and Procypris, rather than among the Barbinae, as previously suggested on morphological grounds.
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resource, Yunnan University, Kunming 650091, China. wuxiaoyun79@gmail.com
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Shanyuan
authors.initials: S
authors.lastname: Chen
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Zan
authors.forename: Heng
authors.initials: H
authors.lastname: Xiao
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Zhang
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000596:Amino Acids / Q000235:genetics; D000818:Animals; D003530:Cyprinidae / Q000145:classification* / Q000235:genetics*; D050259:Genes, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D054629:Genome, Mitochondrial / Q000235:genetics*; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D009711:Nucleotides
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