Elimination of the mycotoxin citrinin production in the industrial important strain Monascus purpureus SM001.
The application of the high-producing pigments industrial strain Monascus purpureus SM001 has been greatly limited by the synchronous production of mycotoxin citrinin. Here we have tried both traditional mutagenesis and metabolic engineering methods to eliminate the production of citrinin. Traditional chemical and physical mutagens were applied to induce mutagenesis, and a bio-screening method based on the antibacterial activity of citrinin against Bacillus subtilis was designed to select mutants. Among the resulting four citrinin-free mutants, only mutant MU2411 can maintain the similar pigments yield. A binary vector system was constructed and successfully disrupted the polyketide synthase gene pksCT in M. purpureus SM001 through the Agrobacterium tumefaciens-mediated transformation. The resulting citrinin-free DeltapksCT mutants maintained the same level of pigments yield. The established Monascus genetic system was comprehensively evaluated and showed high efficiency and specificity, which provides us a potential approach to manipulate and improve industrial Monascus strains.
Jia
Xiao Qin
XQ
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, China.
Xu
Zhi Nan
ZN
Zhou
Li Ping
LP
Sung
Chang Keun
CK
D001412:Bacillus subtilis / Q000254:growth & development; D002953:Citrinin; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D032741:Monascus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D009183:Mycotoxins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D010860:Pigments, Biological / Q000096:biosynthesis / Q000235:genetics; D048630:Polyketide Synthases / Q000235:genetics* / Q000378:metabolism
D005656:Fungal Proteins; D009183:Mycotoxins; D010860:Pigments, Biological; D002953:Citrinin; D048630:Polyketide Synthases