title: Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes.
abstract: Histone acetyltransferases (HATs) and deacetylases (HDACs) function antagonistically to control histone acetylation. As acetylation is a histone mark for active transcription, HATs have been associated with active and HDACs with inactive genes. We describe here genome-wide mapping of HATs and HDACs binding on chromatin and find that both are found at active genes with acetylated histones. Our data provide evidence that HATs and HDACs are both targeted to transcribed regions of active genes by phosphorylated RNA Pol II. Furthermore, the majority of HDACs in the human genome function to reset chromatin by removing acetylation at active genes. Inactive genes that are primed by MLL-mediated histone H3K4 methylation are subject to a dynamic cycle of acetylation and deacetylation by transient HAT/HDAC binding, preventing Pol II from binding to these genes but poising them for future activation. Silent genes without any H3K4 methylation signal show no evidence of being bound by HDACs.
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.forename: Chongzhi
authors.initials: C
authors.lastname: Zang
authors.forename: Kairong
authors.initials: K
authors.lastname: Cui
authors.forename: Dustin E
authors.initials: DE
authors.lastname: Schones
authors.forename: Artem
authors.initials: A
authors.lastname: Barski
authors.forename: Weiqun
authors.initials: W
authors.lastname: Peng
authors.forename: Keji
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000107:Acetylation; D002460:Cell Line; D015870:Gene Expression; D015894:Genome, Human; D051548:Histone Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D008745:Methylation; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000378:metabolism
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; D051548:Histone Acetyltransferases; D012319:RNA Polymerase II; D006655:Histone Deacetylases
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