title: Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells.
abstract: Microtubules are indispensable for Golgi complex assembly and maintenance, which are integral parts of cytoplasm organization during interphase in mammalian cells. Here, we show that two discrete microtubule subsets drive two distinct, yet simultaneous, stages of Golgi assembly. In addition to the radial centrosomal microtubule array, which positions the Golgi in the centre of the cell, we have identified a role for microtubules that form at the Golgi membranes in a manner dependent on the microtubule regulators CLASPs. These Golgi-derived microtubules draw Golgi ministacks together in tangential fashion and are crucial for establishing continuity and proper morphology of the Golgi complex. We propose that specialized functions of these two microtubule arrays arise from their specific geometries. Further, we demonstrate that directional post-Golgi trafficking and cell migration depend on Golgi-associated CLASPs, suggesting that correct organization of the Golgi complex by microtubules is essential for cell polarization and motility.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Miller
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Folkmann
authors.forename: Ana R R
authors.initials: AR
authors.lastname: Maia
authors.forename: Nadia
authors.initials: N
authors.lastname: Efimova
authors.forename: Andrey
authors.initials: A
authors.lastname: Efimov
authors.forename: Irina
authors.initials: I
authors.lastname: Kaverina
mesh_terms: D001692:Biological Transport; D002460:Cell Line; D002465:Cell Movement; D016764:Cell Polarity; D018385:Centrosome / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis
chemical_list: C497850:CLASP1 protein, human; C497851:CLASP2 protein, human; D008869:Microtubule-Associated Proteins; D004398:Dyneins
references.citation: J Cell Sci. 2008 Apr 15;121(Pt 8):1159-64
references.pmid: 18349074
references.citation: Biochim Biophys Acta. 1998 Aug 14;1404(1-2):127-37
references.pmid: 9714774
references.citation: J Cell Sci. 2005 May 15;118(Pt 10):2085-92
references.pmid: 15890984
references.citation: Dev Cell. 2007 Jun;12(6):917-30
references.pmid: 17543864
references.citation: Curr Biol. 2004 Jan 20;14(2):88-98
references.pmid: 14738729
references.citation: J Cell Sci. 1999 Dec;112 ( Pt 23):4243-55
references.pmid: 10564643
references.citation: J Cell Biol. 2005 Jan 3;168(1):141-53
references.pmid: 15631994
references.citation: Curr Biol. 2006 Nov 21;16(22):2259-64
references.pmid: 17113391
references.citation: Mol Biol Cell. 1996 Apr;7(4):631-50
references.pmid: 8730104
references.citation: Cell. 1986 May 9;45(3):329-42
references.pmid: 3516413
references.citation: Biochim Biophys Acta. 1998 Aug 14;1404(1-2):113-26
references.pmid: 9714769
references.citation: Mol Biol Cell. 2008 Jul;19(7):2696-707
references.pmid: 18434598
references.citation: J Biol Chem. 2008 Mar 7;283(10):6085-94
references.pmid: 18156178
references.citation: Development. 2004 Feb;131(4):863-71
references.pmid: 14736750
references.citation: EMBO J. 2009 Apr 22;28(8):1016-28
references.pmid: 19242490
references.citation: J Cell Biol. 1997 Oct 20;139(2):469-84
references.pmid: 9334349
references.citation: Curr Opin Cell Biol. 2003 Feb;15(1):40-7
references.pmid: 12517702
references.citation: Cell. 1992 Mar 6;68(5):829-40
references.pmid: 1547485
references.citation: J Cell Sci. 2007 May 15;120(Pt 10):1717-22
references.pmid: 17502482
references.citation: Nat Cell Biol. 2006 Mar;8(3):238-48
references.pmid: 16489344
references.citation: Curr Opin Cell Biol. 2004 Feb;16(1):106-12
references.pmid: 15037313
references.citation: J Cell Biol. 2005 Jun 20;169(6):929-39
references.pmid: 15955847
references.citation: Histochemistry. 1986;86(2):135-40
references.pmid: 3546220
references.citation: Mol Biol Cell. 2009 Mar;20(6):1728-36
references.pmid: 19158377
references.citation: Exp Cell Res. 1999 Feb 1;246(2):263-79
references.pmid: 9925741
references.citation: Cell. 2004 Aug 6;118(3):323-35
references.pmid: 15294158
references.citation: Dev Cell. 2006 Jul;11(1):21-32
references.pmid: 16824950
references.citation: Trends Cell Biol. 1998 Jan;8(1):16-20
references.pmid: 9695802
references.citation: J Cell Biol. 1996 Oct;135(1):201-14
references.pmid: 8858174
references.citation: J Cell Biol. 1988 Apr;106(4):1213-20
references.pmid: 3283150
references.citation: J Cell Sci. 1999 Dec;112 ( Pt 24):4673-85
references.pmid: 10574715
references.citation: Nat Cell Biol. 2002 Oct;4(10):E236-42
references.pmid: 12360306
references.citation: Mol Biol Cell. 2009 Jan;20(1):209-17
references.pmid: 18946081
references.citation: Nat Rev Mol Cell Biol. 2008 Nov;9(11):874-86
references.pmid: 18946476
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):2070-5
references.pmid: 12538853
references.citation: Annu Rev Cell Dev Biol. 2002;18:379-420
references.pmid: 12142281
references.citation: J Cell Biol. 1984 May;98(5):1730-45
references.pmid: 6725396
references.citation: J Cell Sci. 2004 Mar 15;117(Pt 8):1381-97
references.pmid: 14996905
references.citation: Curr Opin Cell Biol. 2005 Aug;17(4):369-75
references.pmid: 15975779
references.citation: Cell. 2004 Aug 6;118(3):271-2
references.pmid: 15294150
references.citation: Biochem J. 2004 Aug 15;382(Pt 1):13-25
references.pmid: 15186216
references.citation: Int Rev Cytol. 2008;265:111-58
references.pmid: 18275887
references.citation: J Cell Biol. 2002 Oct 28;159(2):245-54
references.pmid: 12391026