Dynamic changes of territories 17 and 18 during EBV-infection of human lymphocytes.
Interphase chromosomes form distinct spatial domains called chromosome territories (CTs). The arrangement of CTs is non-random and correlated with cellular processes such as differentiation. The purpose of this study is to provide some behavior information of CTs during lymphocyte EBV-infection, which is thought to be a general extra-biological model. Three-dimensional fluorescence in situ hybridization (3D-FISH) was performed on human lymphocytes every 24 h over 96 h periods in EBV-infection. Chromosomes 17 and 18 were selected as target territories for similar size and different gene density. The data indicate that the radial position of territories 17 was altered with time, whereas territories 18 showed relative stable localization. The relative CT volume of CTs 18 to 17 also changed with infection. Our study is the first to examine the timely changes of chromatin positioning and folding in EBV-lymphocyte infection. Dynamic changes in position and folding status of target chromosomes reflected an impact of EBV infection on genome stability.
Li
Chunxiang
C
Laboratory of Medical Genetics, Harbin Medical University, 150081 Harbin, China. chunxiangl@gmail.com
Shi
Zhongcheng
Z
Zhang
Liqiu
L
Huang
Yun
Y
Liu
An
A
Jin
Yan
Y
Yu
Yang
Y
Bai
Jing
J
Chen
Dieyan
D
Gendron
Christi
C
Liu
Xinghan
X
Fu
Songbin
S
D053145:Cell Nucleus Size; D055012:Chromosomal Position Effects; D020223:Chromosome Painting; D002886:Chromosomes, Human, Pair 17 / Q000378:metabolism*; D002887:Chromosomes, Human, Pair 18 / Q000378:metabolism*; D020031:Epstein-Barr Virus Infections / Q000378:metabolism*; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism* / Q000821:virology*; D008297:Male; D008677:Metaphase; D009690:Nucleic Acid Conformation
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