Design and evaluation of new analogs of the sweet protein brazzein.
We have previously modeled the interaction of the sweet protein brazzein with the extracellular domains of the sweet taste receptor. Here, we describe the application of that model to the design of 12 new highly potent analogs of brazzein. Eight of the 12 analogs have higher sweetness potency than wild-type brazzein. Results are consistent with our brazzein-receptor interaction model. The model predicts binding of brazzein to the open form of T1R2 in the T1R2-T1R3 heterodimer.
Walters
D Eric
DE
Department of Biochemistry and Molecular Biology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064, USA. eric.walters@rosalindfranklin.edu
Cragin
Tiffany
T
Jin
Zheyuan
Z
Rumbley
Jon N
JN
Hellekant
Göran
G
D000328:Adult; D000368:Aged; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D013549:Sweetening Agents / Q000737:chemistry / Q000378:metabolism*; D013650:Taste Buds / Q000378:metabolism; D055697:Taste Perception; D055815:Young Adult
D010940:Plant Proteins; D043562:Receptors, G-Protein-Coupled; D013549:Sweetening Agents; C090521:brazzein protein, Pentadiplandra brazzeana; C431926:taste receptors, type 1
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