Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers.
Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated that BM-MSCs expressed myogenic (desmin, myogenin, myosin IIa, and alpha-SMA), neurogenic (gamma-enolase, MAP2a,b, c-fos, nestin, GFAP and beta III tubulin), and osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, BMP-2, BMP-4 and type I collagen) markers without stimulation towards differentiation. These expression patterns indicated why these cells can easily differentiate into multiple lineages both in vitro and in vivo. Ultrastructural characteristics of rBM-MSCs showed more developed and metabolically active cells.
Karaoz
Erdal
E
Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, 41380, Kocaeli, Turkey. ekaraoz@hotmail.com
Aksoy
Ayça
A
Ayhan
Selda
S
Sariboyaci
Ayla Eker
AE
Kaymaz
Figen
F
Kasap
Murat
M
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
J Cell Sci. 2003 Aug 15;116(Pt 16):3295-302
12840074
J Tissue Eng Regen Med. 2007 Nov-Dec;1(6):417-24
18247428
Cell Biol Int. 2008 Mar;32(3):344-52
18294871
Mol Cell Biol. 2002 Jul;22(13):4952-64
12052899
J Biomed Sci. 2008 May;15(3):365-74
18256904
Mol Ther. 2007 Jul;15(7):1382-9
17519892
Dis Colon Rectum. 2008 Apr;51(4):411-20
18224375
Calcif Tissue Int. 2008 Mar;82(3):238-47
18305886
Oxf Surv Eukaryot Genes. 1989;6:33-51
2517199
J Vet Med Sci. 2007 Feb;69(2):95-102
17339751
Oncogene. 1990 Jan;5(1):69-73
2108403
Arch Oral Biol. 2007 Jan;52(1):64-73
17049335
Muscle Nerve. 1995 Dec;18(12):1417-26
7477065
Stem Cells Dev. 2008 Aug;17(4):761-73
18393634
Biomaterials. 2007 Jul;28(20):3110-20
17433434
Stem Cells. 2005 Mar;23(3):392-402
15749934
Int Orthop. 2007 Dec;31(6):791-7
17634943
Biomaterials. 2006 Aug;27(24):4333-9
16647114
Int J Mol Med. 2006 Aug;18(2):233-40
16820929
J Histochem Cytochem. 2000 Nov;48(11):1511-20
11036094
Microsc Res Tech. 2005 Feb;66(2-3):132-8
15880496