title: Application of the indirect fluorescent antibody assay in the study of malaria infection in the Yangtze River Three Gorges Reservoir, China.
abstract: BACKGROUND
  China Yangtze Three Gorges Project (TGP) is one of the biggest construction projects in the world. The areas around the Three Gorge Dam has a history of tertian malaria and subtertian malaria epidemic, but there are no overall data about malaria epidemics before the completion of the project. The objective of this study was to get a reliable baseline on malaria infection in the Yangtze River Three Gorges reservoir area and to provide reference data for future studies about the impact of the project on malaria epidemics.
  
  
  METHODS
  Two surveys of malaria infection were carried out in area, at six-month intervals in May and October 2008. About 3,600 dual specimens blood film samples for parasite diagnosis and filter paper blood spots for serology (using the immunofluorescence antibody test) were collected from the general population, including school populations, whenever possible.
  
  
  RESULTS
  The overall percentage of positive response of the same population during post-transmission periods was about twice (1.40/0.72) of that in pre-transmission. Positive individuals under 15 years of age were detected in all the localities.
  
  
  CONCLUSIONS
  A certain extent of malaria infection existed in this area. Additional studies are needed to determine the length of malaria experience, and chemotherapeutic intervention as well as the distribution of main vectors for transmission in this area.
authors.affiliation: National Institute of Parasitic Disease, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, PR China. duoquan2002@yahoo.com.cn
authors.forename: Wang
authors.initials: W
authors.lastname: Duo-Quan
authors.forename: Tang
authors.initials: T
authors.lastname: Lin-Hua
authors.forename: Gu
authors.initials: G
authors.lastname: Zhen-Cheng
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiang
authors.forename: Yang
authors.initials: Y
authors.lastname: Man-Ni
mesh_terms: D000293:Adolescent; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001769:Blood / Q000469:parasitology*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D007223:Infant; D008288:Malaria / Q000453:epidemiology* / Q000276:immunology*; D008853:Microscopy / Q000379:methods; D010961:Plasmodium / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000913:Antibodies, Protozoan
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