Midline crossing and Slit responsiveness of commissural axons require USP33.
Commissural axons cross the ventral midline of the neural tube in a Slit-dependent manner. The underlying molecular mechanisms remain unclear. We found that the deubiquitinating enzyme USP33 interacts with the Robo1 receptor. USP33 was essential for midline crossing by commissural axons and for their response to Slit. Our results reveal a previously unknown role for USP33 in vertebrate commissural axon guidance and in Slit signaling.
Yuasa-Kawada
Junichi
J
Department of Neurology, Lurie Comprehensive Cancer Center, Center for Genetic Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
Kinoshita-Kawada
Mariko
M
Wu
Guan
G
Rao
Yi
Y
Wu
Jane Y
JY
D000818:Animals; D001369:Axons / Q000502:physiology*; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D020439:Growth Cones / Q000502:physiology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013116:Spinal Cord / Q000502:physiology; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism*; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein
D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D043222:Ubiquitin Thiolesterase; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein; C449799:USP33 protein, human
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