Synthesis and physicochemical characterization of a novel amphiphilic polylactic acid-hyperbranched polyglycerol conjugate for protein delivery.
Amphiphilic copolymers with polylactic acid (PLA) chains grafted onto hyperbranched polyglycerol (HPG) have been synthesized and characterized. The copolymer nanoparticles with corona and core structure were formed by self-assembly in aqueous solution. The loading capacity and association efficiency were up to 23% and 86%, respectively. Protein release profiles with different copolymer compositions and BSA concentrations all showed a burst effect followed by a continuous release phase. The released BSA from the copolymer nanoparticles remained in its original structure over a period of 4 days, as testified by circular dichroism spectroscopy. Furthermore, cell viability research suggested good biocompatibility of the copolymer nanoparticles, which have a promising potential for protein delivery system.
Gao
Xiujun
X
Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
Zhang
Xinge
X
Wu
Zhongming
Z
Zhang
Xuejiao
X
Wang
Zhen
Z
Li
Chaoxing
C
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002942:Circular Dichroism; D004337:Drug Carriers; D004339:Drug Compounding; D005990:Glycerol / Q000138:chemical synthesis* / Q000633:toxicity; D007700:Kinetics; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000633:toxicity; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D053758:Nanoparticles; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis* / Q000633:toxicity; D011487:Protein Conformation; D055550:Protein Stability; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility
D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; C043941:polyglycerol; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C033616:poly(lactide); D005990:Glycerol