title: Design of functional metalloproteins.
abstract: Metalloproteins catalyse some of the most complex and important processes in nature, such as photosynthesis and water oxidation. An ultimate test of our knowledge of how metalloproteins work is to design new metalloproteins. Doing so not only can reveal hidden structural features that may be missing from studies of native metalloproteins and their variants, but also can result in new metalloenzymes for biotechnological and pharmaceutical applications. Although it is much more challenging to design metalloproteins than non-metalloproteins, much progress has been made in this area, particularly in functional design, owing to recent advances in areas such as computational and structural biology.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA. yi-lu@illinois.edu
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Natasha
authors.initials: N
authors.lastname: Yeung
authors.forename: Nathan
authors.initials: N
authors.lastname: Sieracki
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Marshall
mesh_terms: D001709:Biotechnology; D015195:Drug Design; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D008667:Metalloproteins / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D015202:Protein Engineering; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D008667:Metalloproteins
references.citation: J Inorg Biochem. 2004 Nov;98(11):1971-4
references.pmid: 15522423
references.citation: Chembiochem. 2007 Nov 5;8(16):1974-80
references.pmid: 17924375
references.citation: Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3760-5
references.pmid: 12552128
references.citation: Inorg Chem. 2006 Dec 11;45(25):9930-40
references.pmid: 17140190
references.citation: Nano Lett. 2005 Nov;5(11):2306-9
references.pmid: 16277473
references.citation: J Am Chem Soc. 2004 Sep 8;126(35):10812-3
references.pmid: 15339144
references.citation: J Am Chem Soc. 2007 Sep 5;129(35):10732-40
references.pmid: 17691729
references.citation: Protein Eng. 2003 Sep;16(9):699-706
references.pmid: 14560056
references.citation: J Am Chem Soc. 2007 Sep 19;129(37):11378-83
references.pmid: 17722917
references.citation: J Am Chem Soc. 2002 Jun 19;124(24):6828-9
references.pmid: 12059195
references.citation: Curr Opin Struct Biol. 2008 Aug;18(4):484-90
references.pmid: 18554898
references.citation: Nature. 2009 Mar 19;458(7236):305-9
references.pmid: 19295603
references.citation: Biochemistry. 2009 Jan 20;48(2):462-70
references.pmid: 19099413
references.citation: Annu Rev Biochem. 1999;68:779-819
references.pmid: 10872466
references.citation: Inorg Chem. 2006 Dec 11;45(25):9941-58
references.pmid: 17140191
references.citation: J Am Chem Soc. 2002 Sep 25;124(38):11234-5
references.pmid: 12236714
references.citation: Curr Opin Chem Biol. 2005 Apr;9(2):118-26
references.pmid: 15811795
references.citation: J Am Chem Soc. 2006 Jun 28;128(25):8320-8
references.pmid: 16787096
references.citation: Angew Chem Int Ed Engl. 2006 Apr 28;45(18):2864-8
references.pmid: 16596690
references.citation: Curr Opin Struct Biol. 1998 Aug;8(4):471-5
references.pmid: 9729739
references.citation: Angew Chem Int Ed Engl. 2009;48(13):2339-41
references.pmid: 19199314
references.citation: FEBS Lett. 1995 May 22;365(1):92-4
references.pmid: 7774723
references.citation: Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16566-71
references.pmid: 18940928
references.citation: J Am Chem Soc. 2002 Mar 13;124(10):2084-5
references.pmid: 11878940
references.citation: J Am Chem Soc. 2003 May 21;125(20):6165-71
references.pmid: 12785848
references.citation: Annu Rev Biochem. 2003;72:249-89
references.pmid: 12626339
references.citation: Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):461-4
references.pmid: 8552661
references.citation: J Mol Biol. 2007 Nov 23;374(2):411-25
references.pmid: 17936298
references.citation: Nature. 1994 Mar 31;368(6470):425-32
references.pmid: 8133888
references.citation: Chem Biol. 1997 Mar;4(3):215-21
references.pmid: 9115415
references.citation: J Am Chem Soc. 2007 Nov 7;129(44):13374-5
references.pmid: 17929927
references.citation: Biochemistry. 1994 Nov 29;33(47):14126-35
references.pmid: 7947824
references.citation: Acc Chem Res. 2004 Oct;37(10):754-62
references.pmid: 15491122
references.citation: Inorg Chem. 2006 Dec 25;45(26):10530-6
references.pmid: 17173408
references.citation: Chembiochem. 2001 Oct 1;2(10):735-40
references.pmid: 11948855
references.citation: J Am Chem Soc. 2005 Apr 27;127(16):5804-5
references.pmid: 15839675
references.citation: J Am Chem Soc. 2002 Jul 10;124(27):8042-54
references.pmid: 12095348
references.citation: Methods Enzymol. 1997;289:3-13
references.pmid: 9353714
references.citation: J Am Chem Soc. 2006 May 31;128(21):6766-7
references.pmid: 16719438
references.citation: Proc Natl Acad Sci U S A. 2004 Apr 13;101(15):5536-41
references.pmid: 15056758
references.citation: Angew Chem Int Ed Engl. 2004 May 3;43(19):2527-30
references.pmid: 15127443
references.citation: Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3629-34
references.pmid: 12655052
references.citation: Biochemistry. 1999 May 4;38(18):5677-83
references.pmid: 10231517
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11566-70
references.pmid: 15292507
references.citation: J Am Chem Soc. 2008 Jul 23;130(29):9188-9
references.pmid: 18572936
references.citation: J Mol Biol. 2002 Aug 30;321(5):923-38
references.pmid: 12206771
references.citation: Biochemistry. 1998 May 19;37(20):7070-6
references.pmid: 9585516
references.citation: J Mol Biol. 2001 Feb 23;306(3):607-28
references.pmid: 11178917
references.citation: Chemistry. 2009;15(15):3761-72
references.pmid: 19229934
references.citation: Curr Opin Biotechnol. 1995 Aug;6(4):411-8
references.pmid: 7579651
references.citation: J Am Chem Soc. 2003 Jul 23;125(29):8760-8
references.pmid: 12862470
references.citation: J Am Chem Soc. 2004 Mar 17;126(10):3006-7
references.pmid: 15012103
references.citation: J Am Chem Soc. 2005 Feb 9;127(5):1346-7
references.pmid: 15686346
references.citation: Inorg Chem. 2004 Aug 9;43(16):4793-5
references.pmid: 15285646
references.citation: Curr Opin Chem Biol. 2005 Apr;9(2):97-103
references.pmid: 15811792
references.citation: J Mol Biol. 2007 Aug 17;371(3):739-53
references.pmid: 17585935
references.citation: Biochemistry. 2001 Jan 30;40(4):938-45
references.pmid: 11170415
references.citation: Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):899-902
references.pmid: 9927665
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11969-74
references.pmid: 17609383
references.citation: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6298-305
references.pmid: 10841536
references.citation: Angew Chem Int Ed Engl. 2008;47(8):1400-4
references.pmid: 18176932
references.citation: J Am Chem Soc. 2008 Oct 8;130(40):13194-5
references.pmid: 18788806
references.citation: Chem Biodivers. 2008 Aug;5(8):1437-48
references.pmid: 18729107
references.citation: Chem Commun (Camb). 2008 Apr 14;(14):1665-7
references.pmid: 18368158
references.citation: Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5869-74
references.pmid: 16581913
references.citation: J Am Chem Soc. 2003 Jul 30;125(30):9030-1
references.pmid: 15369356
references.citation: Eur J Biochem. 1995 Dec 1;234(2):363-81
references.pmid: 8536678
references.citation: Protein Eng. 1998 Apr;11(4):253-61
references.pmid: 9680187
references.citation: Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6404-8
references.pmid: 7541540
references.citation: Science. 1994 Nov 4;266(5186):776-9
references.pmid: 7973629
references.citation: Chem Rev. 2001 Oct;101(10):3047-80
references.pmid: 11710062
references.citation: Science. 1989 Apr 14;244(4901):182-8
references.pmid: 2649980
references.citation: Science. 2006 Jan 27;311(5760):535-8
references.pmid: 16439663
references.citation: Chem Rev. 2004 Feb;104(2):617-49
references.pmid: 14871137
references.citation: Angew Chem Int Ed Engl. 2007;46(44):8414-8
references.pmid: 17886313
references.citation: Acc Chem Res. 2002 Jan;35(1):35-43
references.pmid: 11790087
references.citation: J Am Chem Soc. 2005 Nov 30;127(47):16541-7
references.pmid: 16305243
references.citation: Biochemistry. 1995 Aug 8;34(31):10094-100
references.pmid: 7632681
references.citation: J Inorg Biochem. 2007 Nov;101(11-12):1820-6
references.pmid: 17765314
references.citation: Curr Opin Struct Biol. 2003 Aug;13(4):490-9
references.pmid: 12948779
references.citation: Inorg Chem. 2004 May 3;43(9):2852-8
references.pmid: 15106972
references.citation: Chem Rev. 2001 Oct;101(10):3081-111
references.pmid: 11710063
references.citation: Dalton Trans. 2009 Apr 7;(13):2271-80
references.pmid: 19290357
references.citation: J Pept Res. 2004 Apr;63(4):347-53
references.pmid: 15102052
references.citation: J Biol Chem. 2003 Nov 21;278(47):47269-74
references.pmid: 12970350
references.citation: Angew Chem Int Ed Engl. 2003 Mar 3;42(9):1005-8
references.pmid: 12616550