title: Kinetic study and mathematical modeling of chromium(VI) reduction and microorganism growth under mixed culture.
abstract: The kinetics of chromium(VI) reduction by Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli) was studied under both pure and mixed cultures. Initially, the study of kinetics was performed in pure culture. It was observed that the growth of the two bacteria was both inhibited in the presence of chromium(VI). The maximum specific growth rate (mu( m )) of P. aeruginosa decreased from 2.3942 h(-1) (without Cr(VI)) to 1.8551 h(-1) (with Cr(VI)). Under the mixed culture, the growth of E. coli was inhibited by P. aeruginosa. The maximum specific growth rate (mu( m )) of E. coli decreased form 0.871 h(-1) (in pure culture) to 0.153 h(-1) (in mixed culture). When the concentration of each bacterium was 4.5 x 10(8) cells ml(-1), the half-velocity reduction rate constant (K (C)) and the maximum specific reduction rate constant (v (max)) of chromium(VI) were 80.05 mg chromium(VI) l(-1) and 3.674 mg chromium(VI) cells(-1) h(-1), respectively. The results showed that the simulation appeared in good agreement with the experimental data, supporting the series of mathematical models represented the bacteria growth and chromium(VI) reduction in both pure and mixed cultures usefully.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Changsha, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Kong
authors.forename: Xiandong
authors.initials: X
authors.lastname: Zeng
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Liu
authors.forename: Xuefang
authors.initials: X
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Shenglian
authors.initials: S
authors.lastname: Luo
authors.forename: Wanzhi
authors.initials: W
authors.lastname: Wei
mesh_terms: D002857:Chromium / Q000378:metabolism*; D046508:Culture Techniques; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D008962:Models, Theoretical; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D002857:Chromium
references.citation: J Appl Microbiol. 2000 Jun;88(6):983-91
references.pmid: 10849174
references.citation: Philos Trans R Soc Lond B Biol Sci. 2015 Apr 19;370(1666):
references.pmid: 25750242
references.citation: Curr Microbiol. 2001 Aug;43(2):83-8
references.pmid: 11391468
references.citation: Biotechnol Bioeng. 1993 Jan 5;41(1):129-33
references.pmid: 18601254
references.citation: J Bacteriol. 1980 Jan;141(1):156-63
references.pmid: 6243619
references.citation: Int J Food Microbiol. 2002 Mar;73(2-3):261-74
references.pmid: 11934034
references.citation: Environ Pollut. 2001;115(2):209-18
references.pmid: 11706794
references.citation: Curr Microbiol. 2003 Jul;47(1):51-4
references.pmid: 12783193
references.citation: Curr Microbiol. 2006 Sep;53(3):249-54
references.pmid: 16874546
references.citation: J Bacteriol. 1979 Dec;140(3):888-92
references.pmid: 391805
references.citation: FASEB J. 1995 Dec;9(15):1650-7
references.pmid: 8529846