The evaluation of a user-friendly lateral flow assay for the serodiagnosis of human brucellosis in Kazakhstan.
Serum samples from all patients with culture-confirmed brucellosis including those with chronic disease from Kazakhstan tested positive in the serum agglutination test for titers > or = 1:25 and reacted in the Brucella immunoglobulin M/immunoglobulin G lateral flow assay (LFA) confirming the high sensitivity of these assays. The strong reactivity in the LFA observed for the majority (92.1%) of the samples from the patients with culture-confirmed brucellosis together with the user-friendliness of the assay procedure makes the LFA ideal for the confirmation of brucellosis in endemic areas in Kazakhstan. The Rose Bengal test lacked sensitivity in particular for patients with chronic brucellosis therefore limiting its value as a quick screening assay. The study emphasizes the importance of the LFA as a useful, rapid, and easy-to-perform tool in the diagnostic testing of brucellosis.
Mizanbayeva
Sulushash
S
Republican Sanitary Station and Epidemiological Station, Health Ministry of Kazakhstan Republic, 05008, Almaty, Kazakhstan.
Smits
Henk L
HL
Zhalilova
Katya
K
Abdoel
Theresia H
TH
Kozakov
Stanislaw
S
Ospanov
Kenes S
KS
Elzer
Philip H
PH
Douglas
James T
JT
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D002002:Brucella / Q000276:immunology* / Q000302:isolation & purification; D002006:Brucellosis / Q000175:diagnosis*; D005260:Female; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007623:Kazakhstan; D008297:Male; D008875:Middle Aged; D012698:Serologic Tests / Q000379:methods*; D055815:Young Adult
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M