title: Interaction trap/two-hybrid system to identify interacting proteins.
abstract: The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania.
authors.forename: Erica A
authors.initials: EA
authors.lastname: Golemis
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania.
authors.forename: Ilya
authors.initials: I
authors.lastname: Serebriiskii
authors.affiliation: Wayne State University School of Medicine, Detroit, Michigan.
authors.forename: Russell L
authors.initials: RL
authors.lastname: Finley
authors.affiliation: Wayne State University School of Medicine, Detroit, Michigan.
authors.forename: Mikhail G
authors.initials: MG
authors.lastname: Kolonin Hunt By Interaction Mating
authors.affiliation: Mitotix, Inc, Cambridge, Massachusetts.
authors.forename: Jeno
authors.initials: J
authors.lastname: Gyuris
authors.affiliation: The Molecular Sciences Institute, Berkeley, California.
authors.forename: Roger
authors.initials: R
authors.lastname: Brent
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D010957:Plasmids; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins