Expression and Localization of PRiMA-linked globular form acetylcholinesterase in vertebrate neuromuscular junctions.
Acetylcholinesterase (AChE) is well known to process different molecular forms via the distinct interacting partners. Proline-rich membrane anchor (PRiMA)-linked tetrameric globular AChE (G4 AChE) is mainly found in the vertebrate brain; however, recent studies from our laboratory have suggested its existence at neuromuscular junctions (nmjs). Both muscle and motor neuron express AChE at the nmjs. In muscle, the expression of PRiMA-linked AChE is down-regulated during myogenic differentiation and by motor neuron innervation. As compared with muscle, spinal cord possessed higher total AChE activity and contained PRiMA-linked AChE forms. The spinal cord expression of this form increased during development. More importantly, PRiMA-linked G4 AChE identified as aggregates localized at nmjs. These findings suggest that the restricted localization of PRiMA-linked G4 AChE at the nmjs could be contributed by the pre-synaptic motor neuron and/or the post-synaptic muscle fiber.
Tsim
Karl W K
KW
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, SAR, China.
Leung
K Wing
KW
Mok
Ka Wai
KW
Chen
Vicky P
VP
Zhu
Kevin Y
KY
Zhu
Judy T T
JT
Guo
Ava J Y
AJ
Bi
Cathy W C
CW
Zheng
Ken Y Z
KY
Lau
David T W
DT
Xie
Heidi Q
HQ
Choi
Roy C Y
RC
D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D009046:Motor Neurons / Q000201:enzymology / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000201:enzymology / Q000254:growth & development / Q000294:innervation; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009469:Neuromuscular Junction / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000201:enzymology / Q000648:ultrastructure; D011487:Protein Conformation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011940:Receptor Aggregation / Q000502:physiology; D013116:Spinal Cord / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D013570:Synaptic Membranes / Q000201:enzymology / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C508016:PRiMA1 protein, rat; D012333:RNA, Messenger; D000110:Acetylcholinesterase
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