The role of miR-124a in early development of the Xenopus eye.
It has been reported that miR-124a is abundant in the central nervous system including the eye, and is related to neurogenesis in several species. However, the role of miR-124a in the eye remains unclear. In this study, we show that the expression of miR-124a in Xenopus laevis begins along the neural fold, including the protruding eye anlagen, at a low level at around stage 18; its expression level gradually increases in the neural tube and the eye as embryos develop into later stages and then maintains at a high level in eye to adult stages. Microinjection of a miR-124a precursor at the 8-cell stage leads to malformation of the optic nerve and optic cup, indicating the importance of maintaining low levels of miR-124a during early embryonic development. In addition, miR-124a overexpression markedly down regulates the expression of its predicted targets Lhx2, Hairy2, Gli3, NeuroD1 and Otx2 in/around the eye anlagen, and the interaction of miR-124a with the 3' UTR of Lhx2 represses gene expression as shown by luciferase assays. Moreover, excess miR-124a inhibits cell proliferation in the eye of Xenopus embryos during retinogenesis. These results indicate that miR-124a acts as a post-transcriptional regulator in the genetic network controlling eye morphogenesis and neurogenesis. The mechanism of miR-124a's early interaction with the genetic network may also persist in its later role in the maturing and adult eye and brain.
Qiu
Rong
R
The State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China.
Liu
Kaili
K
Liu
Ying
Y
Mo
Weichuan
W
Flynt
Alex S
AS
Patton
James G
JG
Kar
Amar
A
Wu
Jane Y
JY
He
Rongqiao
R
D000818:Animals; D001483:Base Sequence; D049109:Cell Proliferation; D017931:DNA Primers; D015536:Down-Regulation; D047108:Embryonic Development / Q000235:genetics*; D005123:Eye / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000502:physiology*; D014981:Xenopus / Q000196:embryology*
D017931:DNA Primers; D035683:MicroRNAs
Development. 2003 Apr;130(8):1565-77
12620982
Int J Dev Biol. 2007;51(3):201-9
17486540
Development. 1997 Aug;124(15):2935-44
9247336
Nucleic Acids Res. 2008 Mar;36(4):1153-62
18158304
Stem Cells. 2006 Apr;24(4):857-64
16357340
Development. 2003 Nov;130(21):5155-67
12944429
Development. 2005 Apr;132(7):1737-48
15753216
Nat Genet. 2005 May;37(5):495-500
15806104
Dev Biol. 2005 Sep 1;285(1):101-15
16040025
Science. 2005 Jul 8;309(5732):310-1
15919954
Mol Neurobiol. 2004 Aug;30(1):35-47
15247487
RNA. 2004 Oct;10(10):1507-17
15383676
Nature. 2005 Feb 17;433(7027):769-73
15685193
Science. 2003 Oct 31;302(5646):793-5
14593157
Dev Dyn. 2006 Apr;235(4):1133-41
16470628
Methods Cell Biol. 1991;36:685-95
1811161
Cell. 1999 Aug 6;98(3):341-52
10458609
PLoS Biol. 2004 Nov;2(11):e363
15502875
Mech Dev. 2001 Jan;100(1):115-8
11118895
Dev Dyn. 2006 Sep;235(9):2538-48
16736490
Dev Dyn. 2006 Nov;235(11):3156-65
17013880
PLoS Genet. 2007 Dec;3(12):e215
18085825
Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2422-7
16461918
Genome Biol. 2004;5(9):R68
15345052
Nat Methods. 2006 Jan;3(1):27-9
16369549
J Biol Chem. 2007 Jul 6;282(27):19575-88
17462994
Nat Genet. 2007 Oct;39(10):1191-2
17898777
Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18017-22
16330759
Cancer Res. 2007 Oct 1;67(19):8994-9000
17908999
Dev Biol. 2005 Nov 15;287(2):456-68
16226737
Mol Cell. 2007 Aug 3;27(3):435-48
17679093
Histochem Cell Biol. 2006 Jul;126(1):1-8
16397794
FEBS Lett. 2005 Jan 17;579(2):318-24
15642338
RNA. 2003 Oct;9(10):1274-81
13130141
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9667-72
17535905
Dev Biol. 2008 Oct 15;322(2):368-80
18710660
Neuron. 2001 Nov 20;32(4):591-604
11719201
Cell. 2005 Jan 14;120(1):15-20
15652477
FEBS Lett. 2005 Oct 31;579(26):5911-22
16111679
Genes Dev. 2007 Mar 1;21(5):531-6
17344415
Development. 1999 Jun;126(11):2451-60
10226004
Cell. 2003 Dec 26;115(7):787-98
14697198
Dev Dyn. 2006 Aug;235(8):2185-91
16804893
Genome Biol. 2006;7(9):R85
17002790
RNA. 2008 May;14(5):872-7
18367714
Development. 2006 Feb;133(4):725-35
16436624
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4
16381832
Genes Dev. 2007 Oct 15;21(20):2558-70
17901217
Nature. 2005 Mar 17;434(7031):338-45
15735639
Annu Rev Neurosci. 2006;29:77-103
16776580
Genes Dev. 2007 Apr 1;21(7):744-9
17403776