title: Induced Endocytosis of the Receptor Kinase FLS2.
abstract: Receptor-like kinases (RLKs) that function as pattern-recognition receptors (PRRs) play a key role in plant immune responses. The receptor recognizing flagellin in Arabidopsis, FLS2, is encoded by a membrane resident RLK. FLS2 is involved in preinvasive immunity against bacterial infection. Recent observations revealed that upon flagellin perception FLS2 accumulates in intracellular mobile vesicles and is then degraded. Reminiscent of ligand-induced receptor endocytosis in animals, FLS2 internalization is Wortmannin-sensitive. Mutation of the potentially phosphorylated residue threonine-867 impaired FLS2 endocytosis and flagellin-triggered responses. Furthermore, mutation of a PEST-motif abolished FLS2 endocytosis and downstream flagellin-elicited responses were affected. Thus, FLS2 endocytosis likely involves phosphorylation and ubiquitination events and appears to be interconnected with flagellin signaling. Similarly, TLR4, the mammalian PRR recognizing bacterial lipopolysaccharides (LPS) is internalized in a ligand specific manner. In this addendum, we discuss endocytic processes of plant RLKs focussing on FLS2 and provide a brief comparison with TLR4 endocytosis.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung; Köln, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Salomon
authors.forename: Silke
authors.initials: S
authors.lastname: Robatzek
references.citation: EMBO J. 2006 Feb 22;25(4):683-92
references.pmid: 16467847
references.citation: Plant Cell. 2004 Dec;16(12):3216-29
references.pmid: 15548744
references.citation: Nat Rev Mol Cell Biol. 2002 Aug;3(8):600-14
references.pmid: 12154371
references.citation: Plant Cell. 2006 Feb;18(2):465-76
references.pmid: 16377758
references.citation: Microbes Infect. 2004 Dec;6(15):1382-7
references.pmid: 15596124
references.citation: Nat Immunol. 2003 Dec;4(12):1247-53
references.pmid: 14625549
references.citation: Plant Cell. 2004 May;16(5):1220-34
references.pmid: 15105442
references.citation: Trends Biochem Sci. 2003 Nov;28(11):598-603
references.pmid: 14607090
references.citation: Nature. 2004 Apr 15;428(6984):764-7
references.pmid: 15085136
references.citation: Genes Dev. 2002 Jul 1;16(13):1707-20
references.pmid: 12101128
references.citation: Genes Dev. 2006 Mar 1;20(5):537-42
references.pmid: 16510871
references.citation: Nature. 2002 Feb 28;415(6875):977-83
references.pmid: 11875555
references.citation: Plant Cell. 2001 May;13(5):1155-63
references.pmid: 11340188
references.citation: Cell. 2006 Sep 8;126(5):969-80
references.pmid: 16959575
references.citation: Plant Cell. 2004 Jun;16(6):1604-15
references.pmid: 15155877
references.citation: Cell. 2006 May 19;125(4):749-60
references.pmid: 16713565
references.citation: Adv Drug Deliv Rev. 2003 Nov 14;55(11):1405-19
references.pmid: 14597138