Relocalization of a microtubule-anchoring protein, ninein, from the centrosome to dendrites during differentiation of mouse neurons.
Microtubules in typical cells form radial arrays with their plus-ends pointing toward the cell periphery. In contrast, microtubules in dendrites of neurons are free from centrosomes and have a unique arrangement in which about half have a polarity with a minus-end distal orientation. Mechanisms for generation and maintenance of the microtubule arrangement in dendrites are not well understood. Here, we examined dendritic localization of a centrosomal protein, ninein, which has microtubule-anchoring and stabilizing functions. Immunohistochemical analysis of developing mouse cerebral and cerebellar cortices showed that ninein is localized at the centrosome in undifferentiated neural precursors. In contrast, ninein was barely detected in migrating neurons, such as those in the intermediate layer of the cerebral cortex and the internal granular layer of the cerebellar cortex. High expression was observed in thick dendrite-bearing neurons such as pyramidal neurons of the cerebral cortex and Purkinje neurons in the cerebellar cortex. Ninein was not detected at the centrosome of these cells, but was diffusely present in cell soma and dendrites. In cultured cortical neurons, ninein formed granular structures in soma and dendrites, being not associated with gamma-tubulin. About 60% of these structures showed resistance to detergent and association with microtubules. Our observations suggest that the minus-ends of microtubules may be anchored and stabilized by centrosomal proteins localized in dendrites.
Ohama
Yusaku
Y
Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo, 102-8554, Japan.
Hayashi
Kensuke
K
D000818:Animals; D002454:Cell Differentiation; D018385:Centrosome / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000166:cytology*; D009687:Nuclear Proteins / Q000378:metabolism*
D003598:Cytoskeletal Proteins; C099035:Nin protein, mouse; D009687:Nuclear Proteins
J Cell Biol. 2007 Jan 15;176(2):147-54
17227889
J Cell Biol. 1997 Aug 25;138(4):833-43
9265650
Curr Opin Cell Biol. 2002 Feb;14(1):25-34
11792541
Neuron. 2005 May 5;46(3):383-8
15882636
J Cell Sci. 2005 Apr 15;118(Pt 8):1565-75
15784680
Curr Biol. 2003 Aug 5;13(15):R614-24
12906817
Nat Cell Biol. 2008 Oct;10(10):1172-80
18758451
Mol Cell Biol. 2008 Apr;28(7):2283-94
18212055
J Cell Sci. 2007 Sep 1;120(Pt 17):3064-74
17698918
J Cell Biol. 1993 Jul;122(2):349-59
8320258
J Cell Sci. 2003 Nov 1;116(Pt 21):4419-28
13130100
Neuron. 2000 May;26(2):465-72
10839364
Nat Neurosci. 2004 Feb;7(2):136-44
14703572
Neuropharmacology. 2004 Oct;47(5):677-83
15458839
Cell Motil Cytoskeleton. 2007 May;64(5):347-59
17342761
Arterioscler Thromb Vasc Biol. 2008 Dec;28(12):2123-30
18772498
J Neurobiol. 2004 Jan;58(1):3-17
14598366
Anal Biochem. 1987 Apr;162(1):156-9
2440339
Neuron. 2007 Oct 4;56(1):79-93
17920017
Biochem Biophys Res Commun. 2003 Sep 5;308(4):975-83
12927815
Proc Natl Acad Sci U S A. 1988 Nov;85(21):8335-9
3054884
Science. 2007 Jan 26;315(5811):518-21
17255513
J Cell Sci. 2000 Sep;113 ( Pt 17):3013-23
10934040
Trends Neurosci. 2007 Jun;30(6):276-83
17420058
Cell Motil Cytoskeleton. 2005 Jan;60(1):1-13
15532031
J Cell Biol. 2002 Oct 28;159(2):255-66
12403812
J Cell Biol. 2007 Apr 9;177(1):13-20
17403931
J Neurosci. 2000 Aug 1;20(15):5782-91
10908619
J Cell Sci. 1996 Jan;109 ( Pt 1):179-90
8834802
Neuroscience. 2002;110(1):7-17
11882368
Exp Eye Res. 2007 Nov;85(5):696-713
17888905
Neurosci Lett. 1997 Jun 20;229(1):17-20
9224791
J Cell Sci. 2002 May 1;115(Pt 9):1825-35
11956314
Cell Motil Cytoskeleton. 1993;25(4):336-44
8402954