Proteomic evaluation of wound-healing processes in potato (Solanum tuberosum L.) tuber tissue.
Proteins from potato (Solanum tuberosum L.) tuber slices, related to the wound-healing process, were separated by 2-DE and identified by an MS analysis in MS and MS/MS mode. Slicing triggered differentiation processes that lead to changes in metabolism, activation of defence and cell-wall reinforcement. Proteins related to storage, cell growth and division, cell structure, signal transduction, energy production, disease/defence mechanisms and secondary metabolism were detected. Image analysis of the 2-DE gels revealed a time-dependent change in the complexity of the polypeptide patterns. By microscopic observation the polyalyphatic domain of suberin was clearly visible by D4, indicating that a closing layer (primary suberisation) was formed by then. A PCA of the six sampling dates revealed two time phases, D0-D2 and D4-D8, with a border position between D2 and D4. Moreover, a PCA of differentially expressed proteins indicated the existence of a succession of proteomic events leading to wound-periderm reconstruction. Some late-expressed proteins (D6-D8), including a suberisation-associated anionic peroxidase, have also been identified in the native periderm. Despite this, protein patterns of D8 slices and native periderm were still different, suggesting that the processes of wound-periderm formation are extended in time and not fully equivalent. The information presented in this study gives clues for further work on wound healing-periderm formation processes.
Chaves
Inês
I
Instituto de Tecnologia Química e Biológica (Universidade Nova de Lisboa), Portugal.
Pinheiro
Carla
C
Paiva
Jorge A P
JA
Planchon
Sébastien
S
Sergeant
Kjell
K
Renaut
Jenny
J
Graça
José A
JA
Costa
Gonçalo
G
Coelho
Ana V
AV
Ricardo
Cândido P Pinto
CP
D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry; D010935:Plant Diseases; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry; D035281:Plant Tubers / Q000166:cytology / Q000378:metabolism*; D025341:Principal Component Analysis; D040901:Proteomics; D011198:Solanum tuberosum / Q000166:cytology / Q000378:metabolism*
D010455:Peptides; D010940:Plant Proteins