title: Stage-specific susceptibility of human erythroblasts to Plasmodium falciparum malaria infection.
abstract: Malaria parasites are known to invade and develop in erythrocytes and reticulocytes, but little is known about their infection of nucleated erythroid precursors. We used an in vitro cell system that progressed through basophilic, polychromatic, orthochromatic, and reticulocyte stages to mature erythrocytes. We show that orthochromatic cells are the earliest stages that may be invaded by Plasmodium falciparum, the causative agent of fatal human malaria. Susceptibility to invasion is distinct from intracellular survival and occurs at a time of extensive erythroid remodeling. Together these data suggest that the potential for complexity of host interactions involved in infection may be vastly greater than hitherto realized.
authors.affiliation: Center for Rare and Neglected Diseases, University of Notre Dame, South Bend, IN 46556, USA. ptamez@nd.edu
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Tamez
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Sebastian
authors.initials: S
authors.lastname: Fernandez-Pol
authors.forename: Kasturi
authors.initials: K
authors.lastname: Haldar
authors.forename: Amittha
authors.initials: A
authors.lastname: Wickrema
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D004900:Erythroblasts / Q000469:parasitology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions; D006801:Humans; D016778:Malaria, Falciparum / Q000209:etiology / Q000469:parasitology*; D020411:Oligonucleotide Array Sequence Analysis; D010963:Plasmodium falciparum / Q000254:growth & development / Q000472:pathogenicity*; D012156:Reticulocytes / Q000469:parasitology*
chemical_list: D015415:Biomarkers
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