title: SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods.
abstract: Antibody-conjugated gold nanorods (GNRs) have been used for the targeting and imaging of specific cancer markers expressed on the surface membrane of cancer cells. GNRs with various aspect ratios were fabricated, and their surface-enhanced Raman scattering enhancement effects were evaluated. To attach the GNRs selectively onto the targets in cancer cells, specific antibodies were immobilized on the surface of GNRs using the layer-by-layer deposition method. First, Raman reporter molecules, mercaptopyridine, were attached to the surface of GNRs, and their surface charge was modified using poly(sodium 4-styrene-sulfonate) to make the surface charge negative. Then, anti-rabbit IgGs were immobilized onto the surface of the GNRs by electrostatic interactions. HER2 markers, expressed on the cell surface, were treated with anti-HER2 primary antibodies. Finally, the functionalized nanoprobes, conjugated with secondary antibodies, were attached to the markers on cancer cells by antibody-antibody interactions. In the present study, MCF7 cells overexpressing breast cancer marker HER2 were used as the optical imaging targets. Our experimental results demonstrate the potential feasibility of antibody-conjugated GNRs for the highly sensitive targeting and imaging of biomarkers expressed on the surface membrane of cancer cells.
authors.affiliation: Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Park
authors.forename: Sangyeop
authors.initials: S
authors.lastname: Lee
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Shin
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Son
authors.forename: Chil Hwan
authors.initials: CH
authors.lastname: Oh
authors.forename: Joon Myong
authors.initials: JM
authors.lastname: Song
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
mesh_terms: D000906:Antibodies; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D015854:Up-Regulation
chemical_list: D000906:Antibodies; D015415:Biomarkers; D006046:Gold; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human