Specific post-transcriptional inhibition of mRNA for ligand binding chain of IgE high affinity receptor.
IgE high affinity receptor (FcεRI) plays an important role in triggering type I allergic reactions. In this study, we have investigated the ability of four synthetic and sequence-specific RNA interfering antisense oligodeoxynucleotides (AS-ODNs) to reduce the expression of FcεRIα gene in granulocytes of allergy sufferers in vitro. Only AS1 out of four AS-ODNs specifically inhibited the FcεRIα gene expression and the dose response assay revealed that AS1 was capable of specific inhibition of target mRNA expression over a linear concentration range without affecting the expression of house keeping genes such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Together, these results indicate that sequence-specific RNA interfering ODNs can be effectively used to silence the expression of key genes like IgE high affinity receptor that are involved in chronic inflammatory diseases.
Rana
Sobia
S
Molecular and Cell Biology Laboratory, Department of Physiology and Cell Biology, University of Health Sciences (UHS), Khayaban-e-Jamia Punjab, Lahore, 54600, Pakistan. ranacemb@yahoo.com
Waheed
Ishrat
I
Imran
Muhammad
M
D001483:Base Sequence; D001681:Biological Assay; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D008024:Ligands; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D017455:Receptors, IgE / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D018076:DNA, Complementary; C543355:FcepsilonRI alpha-chain, human; D008024:Ligands; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E
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