title: Further Characterization of Calcineurin B-Like Protein and Its Interacting Partner CBL-Interacting Protein Kinase from Pisum sativum.
abstract: The recently discovered calcium sensor calcineurin B-like proteins (CBLs), and their interacting partners CBL-interacting protein kinases (CIPKs), have emerged as a key network in response to stress and calcium signaling in plants. The studies on CBL and CIPK are so far mainly restricted to Arabidopsis and work on dissecting this pathway in higher plants is inadequate. Our recent studies revealed that calcium sensor CBL from pea gets phosphorylated by pea CIPK. Furthermore, the transcript levels of both the CBL and CIPK from pea were coordinately upregulated in response to various stresses including high salinity, cold, wounding, salicyclic acid and calcium but not to abscisic acid and dehydration. Here we report the results on the computational analysis of EF hands of PsCBL protein, which indicate that it contains all the functional domains required for calcium binding activity. We have also focused on homology based computational modeling of PsCBL and PsCIPK proteins using AtCBL2 and Chk1 as templates respectively, which suggested the high degree of conservation between AtCBL2, PsCBL and OsCBL and between Chk1, PsCIPK and AtCIPK18. Furthermore, the direct interaction of PsCBL and PsCIPK mutant proteins was studied by the yeast 2-hybrid system, which confirmed that FISL domain is an important interaction module required for PsCBL and PsCIPK interaction.
authors.affiliation: Plant Molecular Biology Group; International Centre for Genetic Engineering and Biotechnology; New Delhi India.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
authors.forename: Shilpi
authors.initials: S
authors.lastname: Mahajan
references.citation: Plant Signal Behav. 2007 Mar;2(2):79-85
references.pmid: 19516972
references.citation: Science. 2007 Mar 9;315(5817):1423-6
references.pmid: 17347443
references.citation: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3730-4
references.pmid: 10725382
references.citation: J Biol Chem. 2003 Oct 24;278(43):42240-6
references.pmid: 12871972
references.citation: J Biol Chem. 2002 Nov 29;277(48):46609-15
references.pmid: 12244092
references.citation: Plant Cell. 2002;14 Suppl:S401-17
references.pmid: 12045291
references.citation: Nat Rev Mol Cell Biol. 2005 Jul;6(7):555-66
references.pmid: 16072038
references.citation: Science. 1998 Jun 19;280(5371):1943-5
references.pmid: 9632394
references.citation: Plant Cell. 2005 Aug;17(8):2142-55
references.pmid: 16061961
references.citation: Arch Biochem Biophys. 2005 Dec 15;444(2):139-58
references.pmid: 16309626
references.citation: Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4718-23
references.pmid: 10200328
references.citation: FEBS J. 2006 Mar;273(5):907-25
references.pmid: 16478466
references.citation: Plant Cell. 2002;14 Suppl:S389-400
references.pmid: 12045290