Synthesis and biological evaluation of a novel cardiolipin affinity matrix.
Cardiolipin (1) is a dimeric phospholipid found in the mitochondrial membranes of both plants and animals. In order to understand better its role, we report the preparation of an immobilised analogue (2) using phosphoramidite chemistry; the probe has been used successfully to bind a recombinant protein containing a cardiolipin-binding domain.
Johns
Melloney K
MK
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Yin
Meng-Xin
MX
Conway
Stuart J
SJ
Robinson
Diane E J E
DE
Wong
Leon S-M
LS
Bamert
Rebecca
R
Wettenhall
Richard E H
RE
Holmes
Andrew B
AB
D000818:Animals; D002308:Cardiolipins / Q000737:chemistry* / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000737:chemistry; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012685:Sepharose
D002308:Cardiolipins; D009943:Organophosphorus Compounds; D011994:Recombinant Proteins; C434331:phosphoramidite; D012685:Sepharose; C061570:protein kinase N; D011493:Protein Kinase C