Protective effect of resveratrol in severe acute pancreatitis-induced brain injury.
OBJECTIVE
The aim of this study was to study the effects of resveratrol on severe acute pancreatitis (SAP)-induced brain injury.


METHODS
Ninety-six male Sprague-Dawley rats were randomly divided into 4 equal groups: sham operation, SAP, resveratrol-treated (RES), and dexamethasone-treated. Each group was evaluated at 3, 6, and 12 hours. Levels of serum myelin basic protein and zonula occludens 1 (Zo-1) were determined by enzyme-linked immunosorbent assay. The brain and pancreatic tissues were examined using electron microscopy. Expressions of Bax, Bcl-2, and caspase-3 were observed using immunohistochemistry, reverse transcriptase polymerase chain reaction, and Western blotting. Cytochrome c was detected using Western blotting alone.


RESULTS
Myelin basic protein and Zo-1 levels of the RES group were lower than the SAP group at all time points (P < 0.05). The RES group had significantly improved pathologic brain, increase in Bcl-2 expression, and decrease in Bax and caspases-3 expressions compared with the SAP group.


CONCLUSIONS
The degradation of Zo-1 is involved in the pathophysiology of brain injury in SAP; MBP can be used as a marker of brain injury in SAP. The protective effect of resveratrol might be associated with the up-regulation of Bcl-2 and down-regulation of Bax and caspase-3.
Jha
Rajiv Kumar
RK
Department of Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China. rajiv12@hotmail.com
Ma
Qingyong
Q
Sha
Huanchen
H
Palikhe
Muna
M
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D001930:Brain Injuries / Q000209:etiology / Q000517:prevention & control*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D008854:Microscopy, Electron; D004676:Myelin Basic Protein / Q000097:blood; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000150:complications*; D010750:Phosphoproteins / Q000097:blood; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology; D062826:Zonula Occludens-1 Protein; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
C494828:Bax protein, rat; D008565:Membrane Proteins; D004676:Myelin Basic Protein; D018696:Neuroprotective Agents; D010750:Phosphoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D013267:Stilbenes; C569044:Tjp1 protein, rat; D014665:Vasodilator Agents; D062826:Zonula Occludens-1 Protein; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D053148:Caspase 3; D000077185:Resveratrol