title: Effects of exogenous annexin-1 on lipopolysaccharide-induced proliferation and reactive oxygen species production partially through modulation of CRAC channels but independent of NF-kappaB pathway.
abstract: OBJECTIVE
  To investigate the effects of exogenous annexin-1 (ANXA1) on lipopolysaccharide(LPS)-induced proliferation, reactive oxygen species (ROS) production, and calcium signal transduction in RAW264.7 macrophages.
  
  
  METHODS
  RAW264.7 macrophages were treated with or without LPS in the absence or presence of ANXA1. The proliferation effects were detected by Cell Counting Kit-8 assay. ROS were quantified by flow cytometry and fluorescence microscopy. Intracellular Ca(2+) concentration ([Ca(2+)](i)) was analyzed by laser confocal scanning microscopy. IkappaBalpha degradation and NF-kappaB translocation were tested by Western blot.
  
  
  RESULTS
  Exogenous ANXA1 inhibited LPS-induced proliferation and ROS production in a dose-dependent manner. LPS evoked [Ca(2+)](i) increase through CRAC channels, and ANXA1 suppressed LPS-induced [Ca(2+)](i) increase in a dose-dependent manner. The CRAC channels were associated with LPS-induced proliferation and ROS production. Exogenous ANXA1 had no effect on LPS-induced IkappaB degradation and NF-kappaB translocation.
  
  
  CONCLUSIONS
  ANXA1 inhibited LPS-induced proliferation and ROS production in RAW264.7 macrophages partially through modulation of CRAC channels but independent of the NF-kappaB pathway.
authors.affiliation: Department of Pathophysiology, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China. xuleiming80215@163.com
authors.forename: Lei-Ming
authors.initials: LM
authors.lastname: Xu
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Jin
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Lin
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Du-Yun
authors.initials: DY
authors.lastname: Ye
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019284:Thapsigargin / Q000378:metabolism
chemical_list: D017305:Annexin A1; D015220:Calcium Channels; D004791:Enzyme Inhibitors; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D017382:Reactive Oxygen Species; D000072000:NF-KappaB Inhibitor alpha; D019284:Thapsigargin; D002118:Calcium
references.citation: Physiol Rev. 2005 Apr;85(2):757-810
references.pmid: 15788710
references.citation: World J Gastroenterol. 2006 Apr 14;12(14):2195-200
references.pmid: 16610020
references.citation: Pharmacol Res. 1994 Jul;30(1):53-9
references.pmid: 7831195
references.citation: Am J Physiol Cell Physiol. 2003 Feb;284(2):C316-30
references.pmid: 12529250
references.citation: FASEB J. 2003 Aug;17(11):1544-6
references.pmid: 12824302
references.citation: J Cell Sci. 1997 May;110 ( Pt 10):1199-213
references.pmid: 9191044
references.citation: Arthritis Rheum. 1999 Jul;42(7):1538-44
references.pmid: 10403283
references.citation: Br J Pharmacol. 2001 May;133(2):217-28
references.pmid: 11350857
references.citation: J Immunol. 2004 Jun 15;172(12):7669-76
references.pmid: 15187149
references.citation: Free Radic Biol Med. 2000 May 15;28(10):1456-62
references.pmid: 10927169
references.citation: J Cell Sci. 1998 May;111 ( Pt 10):1405-18
references.pmid: 9570758
references.citation: Br J Pharmacol. 1991 Jan;102(1):7-9
references.pmid: 1828384
references.citation: Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14535-9
references.pmid: 9826735
references.citation: Trends Pharmacol Sci. 2003 Nov;24(11):574-9
references.pmid: 14607080
references.citation: Cancer Res. 2000 Feb 15;60(4):1002-8
references.pmid: 10706116
references.citation: Am J Physiol Heart Circ Physiol. 2001 Feb;280(2):H746-55
references.pmid: 11158974
references.citation: J Cell Sci. 2001 Jun;114(Pt 12):2223-9
references.pmid: 11493662
references.citation: Blood. 2008 Sep 15;112(6):2512-9
references.pmid: 18594025
references.citation: Int J Immunopharmacol. 2000 Aug;22(8):635-44
references.pmid: 10988358
references.citation: Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3473-7
references.pmid: 7536934
references.citation: Cold Spring Harb Symp Quant Biol. 1988;53 Pt 2:927-33
references.pmid: 3267114
references.citation: J Immunol. 1993 Feb 1;150(3):992-9
references.pmid: 8423349
references.citation: Am J Pathol. 2005 Jun;166(6):1607-17
references.pmid: 15920146
references.citation: J Immunol. 2007 Dec 15;179(12):8533-43
references.pmid: 18056401
references.citation: Am J Physiol Lung Cell Mol Physiol. 2003 Dec;285(6):L1233-45
references.pmid: 12909593
references.citation: Inflamm Res. 2004 Apr;53(4):125-32
references.pmid: 15060718
references.citation: Nat Med. 1996 Nov;2(11):1259-62
references.pmid: 8898757
references.citation: News Physiol Sci. 2003 Apr;18:60-4
references.pmid: 12644621
references.citation: Nat Rev Immunol. 2004 Mar;4(3):181-9
references.pmid: 15039755
references.citation: Biochem Soc Trans. 1990 Dec;18(6):1237-8
references.pmid: 2150953
references.citation: Proc Natl Acad Sci U S A. 1989 May;86(9):3428-32
references.pmid: 2470103
references.citation: Physiol Rev. 2002 Apr;82(2):331-71
references.pmid: 11917092
references.citation: Cell Calcium. 2001 Aug;30(2):141-50
references.pmid: 11440471