DHEA effects on myocardial Akt signaling modulation and oxidative stress changes in aged rats.
The secretion of DHEA-synthesized mainly in the adrenal cortex-increases in the postnatal aging, peaks in the twenties and decreases with age afterwards. Exogenous DHEA can exert a dual effect depending on dose and on tissue. Akt is a serine/threonine kinase whose activity has been seen as an interventional approach for cardiomyopathic damage resulting from aging changes. In order to evaluate DHEA effects over myocardial Akt protein expression associated to oxidative stress markers during aging, male Wistar rats (3 and 18 months) were assigned into two groups: control or DHEA (10mg/kg, subcutaneously, for 5 weeks). In the aged group, we found increased lipid peroxidation and glutathione-S-transferase activity. DHEA produced an increase in p-Akt protein expression and a decrease in SOD activity in both ages. Akt pathway activation might be related to changes in oxidative stress parameters according to age.
Jacob
Maria Helena Vianna Metello
MH
Laboratório de Interação Neuro-Humoral, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Av. Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, Brazil. mhvmjacob@hotmail.com
Janner
Daiane da Rocha
Dda R
Jahn
Matheus Parmegiani
MP
Kucharski
Luiz Carlos Rios
LC
Belló-Klein
Adriane
A
Ribeiro
Maria Flavia Marques
MF
D000375:Aging / Q000187:drug effects / Q000502:physiology; D000818:Animals; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000819:agonists / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D003687:Dehydroepiandrosterone; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D051057:Proto-Oncogene Proteins c-akt