title: In Vitro and In Vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella.
abstract: Punica granatum is commonly used in Korea as a traditional medicine for the treatment of pathogenic bacteria. In this study, we investigated the in vitro and in vivo antimicrobial activity of P. granatum peel EtOH extract (PGPE) against 16 strains of Salmonella. The minimal inhibitory concentrations of PGPE were in the range of 62.5-1000 x03BCg mL(-1). In addition, the in vivo antibacterial activity of the PGPE extract was examined in a S. typhimurium infection mouse model. Mice were initially infected with S. typhimurium and then with PGPE. The extract was found to have significant effects on mortality and the numbers of viable S. typhimurium recovered from feces. Although clinical signs and histological damage were rarely observed in the treated mice, the untreated controls showed signs of lethargy and histological damage in the liver and spleen. Taken together, the results of this study indicate that PGPE has the potential to provide an effective treatment for salmonellosis.
authors.affiliation: College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.
authors.forename: Jang-Gi
authors.initials: JG
authors.lastname: Choi
authors.forename: Ok-Hwa
authors.initials: OH
authors.lastname: Kang
authors.forename: Young-Seob
authors.initials: YS
authors.lastname: Lee
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Chae
authors.forename: You-Chang
authors.initials: YC
authors.lastname: Oh
authors.forename: Obiang-Obounou
authors.initials: OO
authors.lastname: Brice
authors.forename: Min-San
authors.initials: MS
authors.lastname: Kim
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Hun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Shin
authors.forename: Jung-Rae
authors.initials: JR
authors.lastname: Rho
authors.forename: Dong-Yeul
authors.initials: DY
authors.lastname: Kwon
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