Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments.
As the molecular origins of disease are better understood, the need for affordable, rapid, and automated technologies that enable microscale molecular diagnostics has become apparent. Widespread use of microsystems that perform sample preparation and molecular analysis could ensure that the benefits of new biomedical discoveries are realized by a maximum number of people, even those in environments lacking any infrastructure. While progress has been made in developing miniaturized diagnostic systems, samples are generally processed off-device using labor-intensive and time-consuming traditional sample preparation methods. We present the concept of an integrated programmable general-purpose sample analysis processor (GSAP) architecture where raw samples are routed to separation and analysis functional blocks contained within a single device. Several dielectrophoresis-based methods that could serve as the foundation for building GSAP functional blocks are reviewed including methods for cell and particle sorting, cell focusing, cell ac impedance analysis, cell lysis, and the manipulation of molecules and reagent droplets.
Gascoyne
Peter R C
PR
Department of Molecular Pathology, M.D., Anderson Cancer Center, University of Texas, Houston, TX 77030 USA.
Vykoukal
Jody V
JV
Biochim Biophys Acta. 1986 Sep 25;861(1):122-30
3092858
Phys Rev E Stat Nonlin Soft Matter Phys. 2001 Aug;64(2 Pt 2):026605
11497728
Biophys J. 1997 Apr;72(4):1887-99
9083692
Biochim Biophys Acta. 1991 Jan 9;1061(1):111-20
1899800
J Membr Biol. 1998 Jan 1;161(1):21-32
9430618
Lab Chip. 2001 Sep;1(1):76-82
15100895
Biotechnol Bioeng. 1999 Sep 5;64(5):509-18
10404231
Biochim Biophys Acta. 1987 Jun 12;900(1):45-55
3297146
J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):193-203
8044280
J Hematother Stem Cell Res. 1999 Oct;8(5):481-90
10791899
Biochim Biophys Acta. 1993 Jun 11;1157(2):127-40
8507649
Electrophoresis. 2002 Jul;23(13):1973-83
12210248
Ann Biol Clin (Paris). 1996;54(6):253-7
8949422
Biomed Sci Instrum. 1997;34:157-62
9603031
Langmuir. 2003 Mar 18;19(6):2425-2433
20686640
Lab Chip. 2002 May;2(2):96-101
15100841
Biochim Biophys Acta. 1999 Jan 4;1426(1):53-68
9878687
IEEE Trans Biomed Eng. 2001 Aug;48(8):921-30
11499529
Anal Chem. 2002 Jul 15;74(14):3362-71
12139041
Biochim Biophys Acta. 1997 Nov 15;1349(2):182-96
9421190
Anal Chem. 2000 Feb 15;72(4):832-9
10701270
Lab Chip. 2002 May;2(2):70-5
15100837
Biosci Rep. 1986 Nov;6(11):973-82
3580521
Eur Biophys J. 2001 Aug;30(4):268-72
11548129
Phys Med Biol. 1992 Jul;37(7):1499-517
1631195
Biochim Biophys Acta. 1990 Jan 29;1021(2):191-200
2302395
Biophys J. 1992 Dec;63(6):1632-42
19431866
IEEE Trans Ind Appl. 1997;33(3):670-678
20011619
Science. 1993 Jun 4;260(5113):1456-65
8502990
Biophys J. 1999 May;76(5):2833-42
10233099
Biochim Biophys Acta. 1995 Aug 17;1245(1):85-93
7654770
Anal Chem. 1998 Jul 1;70(13):2607-12
9666729
Biochim Biophys Acta. 1997 Jan 31;1323(2):240-52
9042346
Hum Reprod. 1998 Jan;13(1):136-41
9512245
J Theor Biol. 1979 May 21;78(2):251-69
573830
Anal Chem. 1992 Jan 1;64(1):6-15
1736679
Biophys J. 1998 May;74(5):2689-701
9591693
Biochim Biophys Acta. 1996 Jun 13;1282(1):76-84
8679663
Microbios. 1993;73(296):165-72
8469175
Phys Med Biol. 1995 Nov;40(11):1789-806
8587932
Biophys J. 1997 Aug;73(2):1118-29
9251828
Biophys J. 1995 Jan;68(1):364-72
7711263
Biophys J. 2000 May;78(5):2680-9
10777764
Biochim Biophys Acta. 1994 Aug 3;1193(2):330-44
8054355
Anal Chem. 2002 Aug 15;74(16):3984-90
12199564
Biochim Biophys Acta. 2002 Aug 31;1564(2):412-20
12175924
Biophys J. 1998 Apr;74(4):2114-20
9545070
Biophys J. 1999 Jun;76(6):3307-14
10354456
Biochim Biophys Acta. 2002 Aug 31;1564(2):449-58
12175928
Cell Biophys. 1984 Dec;6(4):233-51
6085558
Biophys J. 2002 Oct;83(4):2170-9
12324434
Int J Adv Manuf Syst. 2000;3(2):1-12
22025905
Biophys J. 1991 Oct;60(4):749-60
1835890
Electrophoresis. 2002 Jul;23(13):2057-63
12210259
Phys Med Biol. 1987 Aug;32(8):933-70
3306721
Anal Chem. 1998 May 1;70(9):1909-15
9599586
J Biotechnol. 1994 Jan 15;32(1):29-37
7764449
Biosens Bioelectron. 1999 Mar 15;14(3):341-51
10230035
J Biotechnol. 1996 Nov 1;51(2):175-80
8987883
Arch Dermatol Res. 1983;275(3):141-3
6614991
Biophys J. 1997 Aug;73(2):1103-9
9251826
Biotechnol Prog. 2001 Nov-Dec;17(6):1145-55
11735453
J Membr Biol. 1993 Feb;132(1):27-40
8459447
Science. 1987 Mar 20;235(4795):1517-20
3547653
Biotechnol Bioeng. 1999 Sep 5;64(5):519-26
10404232
Biophys J. 1999 Jul;77(1):516-25
10388776
Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):860-4
7846067
Phys Rev Lett. 1985 Mar 25;54(12):1245-1248
10030975