A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis.
Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing technique permits to differentiate Y. pestis isolates and turned out to be robust, reproducible, and cheap.
Ciammaruconi
Andrea
A
Army Medical Research Center, 00184 Rome, Italy. andrea.ciammaruconi@gmail.com
Grassi
Saverio
S
Faggioni
Giovanni
G
De Santis
Riccardo
R
Pittiglio
Valentina
V
D'Amelio
Raffaele
R
Vergnaud
Gilles
G
Lista
Florigio
F
D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D044366:Transition Temperature; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics*
D004269:DNA, Bacterial