Molecular characterization and genetic diversity analysis of Jatropha curcas L. in India using RAPD and AFLP analysis.
Jatropha curcas L. belongs to family Euphorbiaceae, native to South America and widely distributed in South and Central America, attained significant importance for its seed oil which can be converted to biodiesel, a renewable energy source alternative to conventional petro-diesel. Very few attempts were made to understand the extent of genetic diversity that exists in J. curcas. Therefore, the present investigation was undertaken to asses the genetic diversity among 28 diverse germplasm collected from distinct geographical areas in India. The overall percentage of polymorphism (PP) was found to be 50.70 and 60.95 by RAPD and AFLP, respectively. The mean PP was found to be 9.72 and 20.57 by RAPD and AFLP, respectively. The mean genetic similarity was observed to be 0.89 by RAPD and 0.88 by AFLP. Among the germplasm JCI20 found to be the most diverged one. The dendrogram analysis of RAPD and AFLP data showed good congruence, but better resolution and more polymorphism was observed with AFLP. When the dendrogram of RAPD was compared with AFLP dendrogram, the major clustering pattern was found to be similar; however, changes in minor grouping were observed. In both RAPD and AFLP analysis clustering of germplasm did not show any correlation with the geographical area of collection. Low genetic diversity observed in J. curcas and the clustering pattern indicates that the distribution of species might have happened through anthropogenic activity and warrants the need for widening the genetic base. The present study will provide pavement for further intra-population studies on narrow geographical areas, to understand the population genetic structure, phylogeography, molecular ecological studies. The marker information and the characterized germplasm help in further improvement of the species through marker assisted breeding programs.
Pamidimarri
D V N Sudheer
DV
Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat, 364002, India.
Mastan
Shaik G
SG
Rahman
Hifzur
H
Reddy
Muppala P
MP
D054458:Amplified Fragment Length Polymorphism Analysis; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D005843:Geography; D007194:India; D031286:Jatropha / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000235:genetics
D017931:DNA Primers
Nucleic Acids Res. 1995 Nov 11;23(21):4407-14
7501463
Theor Appl Genet. 2004 Nov;109(8):1687-93
15368042
Theor Appl Genet. 2003 May;106(7):1316-25
12748784
Theor Appl Genet. 1994 Jun;88(3-4):417-22
24186028
Plant Sci. 2009 Apr;176(4):505-13
26493140
Nucleic Acids Res. 1990 Nov 25;18(22):6531-5
1979162
Mol Biol Rep. 2009 Jul;36(6):1357-64
18642099
Evolution. 1985 Jul;39(4):783-791
28561359
Mol Ecol Resour. 2009 Jan;9(1):431-3
21564671
Am J Bot. 2000 Apr;87(4):591-6
10766731
Nature. 2007 Oct 11;449(7163):652-5
17968401
Mol Ecol. 2004 Jan;13(1):81-6
14653790
Mol Biol Rep. 2009 May;36(5):901-7
18446444
Mol Biol Rep. 2009 Sep;36(7):1929-35
18987989