title: Molecular identification of oriental medicinal plant Anemarrhena asphodeloides Bunge ('Jimo') by multiplex PCR.
abstract: Anemarrhena asphodeloides Bunge (Jimo) is one of the most popular and valuable plant species in many countries, including China, Korea, and Japan. The current commercial products such as Sagan, Chog-chag-yag, and Shig-ban-pung which are most similar to Jimo roots, were used for more reliable authentication method. Polymerase chain reaction (PCR) analysis of the trnL-F region has been proved to be an appropriate method for the identification of species in the A. asphodeloides genus. A single nucleotide polymorphism (SNP) has been identified in Jimo, Sagan, Chog-chag-yag, and Shig-ban-pung. The specific PCR primers were designed from the SNP to differentiate the A. asphodeloides (Jimo) from Sagan, Chog-chag-yag, and Shig-ban-pung via multiplex PCR. The established multiplex-PCR method for the rapid detection of the Jimo in a single reaction was determined to be effective for the differentiation of Jimo (A. asphodeloides). We therefore present an effective method for the genetic identification of the A. asphodeloides.
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 449-701, South Korea.
authors.forename: Baigalmaa
authors.initials: B
authors.lastname: Jigden
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.forename: Narantuya
authors.initials: N
authors.lastname: Samdan
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D031403:Anemarrhena / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D056426:Genetic Loci; D008518:Medicine, East Asian Traditional; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
references.citation: Am J Bot. 2005 Jan;92(1):142-66
references.pmid: 21652394
references.citation: Plant Mol Biol. 1991 Nov;17(5):1105-9
references.pmid: 1932684
references.citation: Planta Med. 2001 Nov;67(8):781-3
references.pmid: 11731932
references.citation: Planta Med. 2008 Jun;74(8):889-92
references.pmid: 18537077
references.citation: Biol Pharm Bull. 2008 Jan;31(1):135-8
references.pmid: 18175956
references.citation: Phytother Res. 1999 Sep;13(6):484-8
references.pmid: 10479758
references.citation: J Pharm Biomed Anal. 2007 May 9;44(1):96-100
references.pmid: 17349768
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: J Agric Food Chem. 2008 Jun 11;56(11):3953-9
references.pmid: 18481863
references.citation: Clin Chim Acta. 1999 Nov;289(1-2):79-88
references.pmid: 10556655
references.citation: Biol Pharm Bull. 2007 Jan;30(1):38-43
references.pmid: 17202656
references.citation: Cell Biol Int. 2007 Sep;31(9):887-92
references.pmid: 17400003
references.citation: Biol Pharm Bull. 2005 Sep;28(9):1798-800
references.pmid: 16141565
references.citation: Planta Med. 2000 Jun;66(5):435-8
references.pmid: 10909263
references.citation: Biol Pharm Bull. 2008 Sep;31(9):1806-8
references.pmid: 18758082
references.citation: Phytochemistry. 1999 Mar;50(5):787-91
references.pmid: 10192964
references.citation: Planta Med. 2003 Jun;69(6):584-6
references.pmid: 12865989
references.citation: J Agric Food Chem. 2002 Mar 27;50(7):1871-5
references.pmid: 11902926
references.citation: Biol Pharm Bull. 2003 Dec;26(12):1721-4
references.pmid: 14646178
references.citation: Brain Res. 2005 Oct 26;1060(1-2):26-39
references.pmid: 16226729
references.citation: Arch Pharm Res. 2003 Aug;26(8):601-5
references.pmid: 12967194