title: The microRNA-regulated SBP-Box transcription factor SPL3 is a direct upstream activator of LEAFY, FRUITFULL, and APETALA1.
abstract: When to form flowers is a developmental decision that profoundly impacts the fitness of flowering plants. In Arabidopsis this decision is ultimately controlled by the induction and subsequent activity of the transcription factors LEAFY (LFY), FRUITFULL (FUL), and APETALA1 (AP1). Despite their central importance, our current understanding of the regulation of LFY, FUL, and AP1 expression is still incomplete. We show here that all three genes are directly activated by the microRNA-targeted transcription factor SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 (SPL3). Our findings suggest that SPL3 acts together with other microRNA-regulated SPL transcription factors to control the timing of flower formation. Moreover, the identified SPL activity defines a distinct pathway in control of this vital developmental decision.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018, USA.
authors.forename: Ayako
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: Miin-Feng
authors.initials: MF
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: R Scott
authors.initials: RS
authors.lastname: Poethig
authors.forename: Doris
authors.initials: D
authors.lastname: Wagner
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C097414:AGL8 protein, Arabidopsis; C091535:AP1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C074747:LFY protein, Arabidopsis; D026161:MADS Domain Proteins; D035683:MicroRNAs; D011993:Recombinant Fusion Proteins; C108465:SPL3 protein, Arabidopsis; D014157:Transcription Factors
references.citation: Mech Dev. 2003 Apr;120(4):491-502
references.pmid: 12676326
references.citation: Plant J. 2007 Feb;49(4):683-93
references.pmid: 17217458
references.citation: Development. 2000 Feb;127(4):725-34
references.pmid: 10648231
references.citation: Development. 2006 Sep;133(18):3539-47
references.pmid: 16914499
references.citation: Development. 1997 Oct;124(19):3835-44
references.pmid: 9367439
references.citation: Nature. 1995 Oct 12;377(6549):522-4
references.pmid: 7566148
references.citation: Nature. 1995 Oct 12;377(6549):495-500
references.pmid: 7566146
references.citation: Genetics. 2003 Oct;165(2):821-33
references.pmid: 14573491
references.citation: Dev Cell. 2005 Apr;8(4):517-27
references.pmid: 15809034
references.citation: Cell. 1992 May 29;69(5):843-59
references.pmid: 1350515
references.citation: Development. 2003 Dec;130(24):6001-12
references.pmid: 14573523
references.citation: Plant J. 2008 Sep;55(5):832-43
references.pmid: 18466303
references.citation: Plant J. 1997 Aug;12(2):367-77
references.pmid: 9301089
references.citation: Science. 2003 Jul 18;301(5631):334-6
references.pmid: 12869752
references.citation: Science. 1999 Jul 23;285(5427):582-4
references.pmid: 10417387
references.citation: Gene. 2008 Jul 15;418(1-2):1-8
references.pmid: 18495384
references.citation: Development. 1998 Aug;125(16):3027-35
references.pmid: 9671577
references.citation: Curr Opin Plant Biol. 2001 Feb;4(1):63-8
references.pmid: 11163170
references.citation: Development. 1997 Oct;124(19):3845-53
references.pmid: 9367440
references.citation: Mol Gen Genet. 1996 Jan 15;250(1):7-16
references.pmid: 8569690
references.citation: Science. 2005 Apr 8;308(5719):260-3
references.pmid: 15821093
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3646-51
references.pmid: 18305171
references.citation: Science. 2005 Aug 12;309(5737):1052-6
references.pmid: 16099979
references.citation: Development. 2008 Apr;135(8):1481-91
references.pmid: 18339670
references.citation: Plant Cell. 2005 Oct;17(10):2661-75
references.pmid: 16155177
references.citation: Ann Bot. 2007 Sep;100(3):659-76
references.pmid: 17679690
references.citation: Plant Physiol. 2005 Aug;138(4):2145-54
references.pmid: 16040653
references.citation: Nat Genet. 2008 Dec;40(12):1489-92
references.pmid: 18997783
references.citation: Plant Physiol. 2006 Sep;142(1):280-93
references.pmid: 16861571
references.citation: Development. 2002 May;129(10):2519-27
references.pmid: 11973282
references.citation: Plant Mol Biol. 2008 May;67(1-2):183-95
references.pmid: 18278578
references.citation: Science. 2005 Aug 12;309(5737):1056-9
references.pmid: 16099980
references.citation: Plant Cell. 2008 May;20(5):1231-43
references.pmid: 18492871
references.citation: Annu Rev Plant Biol. 2008;59:573-94
references.pmid: 18444908
references.citation: Nat Genet. 2007 Apr;39(4):544-9
references.pmid: 17369828
references.citation: Genes Dev. 2005 Apr 15;19(8):992-1003
references.pmid: 15833920
references.citation: Plant Cell. 2009 Feb;21(2):403-19
references.pmid: 19244139
references.citation: Mol Biol Evol. 2005 Apr;22(4):1082-94
references.pmid: 15689530
references.citation: Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1775-80
references.pmid: 14736918
references.citation: Trends Plant Sci. 2006 Aug;11(8):375-81
references.pmid: 16843035
references.citation: Physiol Plant. 2004 Mar;120(3):491-500
references.pmid: 15032847
references.citation: Gene. 1999 Sep 3;237(1):91-104
references.pmid: 10524240
references.citation: Plant Mol Biol. 2006 Apr;60(6):855-70
references.pmid: 16724257
references.citation: Genes Dev. 2007 Oct 1;21(19):2371-84
references.pmid: 17908925
references.citation: Development. 2004 Jul;131(14):3357-65
references.pmid: 15226253
references.citation: Nucleic Acids Res. 2007 Jan;35(Database issue):D857-62
references.pmid: 17148485
references.citation: Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100
references.pmid: 17690442
references.citation: Science. 1999 Jul 23;285(5427):585-7
references.pmid: 10417388
references.citation: Science. 2007 Jun 8;316(5830):1452-6
references.pmid: 17525303
references.citation: Plant Cell. 1991 Aug;3(8):771-781
references.pmid: 12324613
references.citation: Plant Physiol. 2001 Dec;127(4):1682-93
references.pmid: 11743113
references.citation: Genes Dev. 2006 Apr 1;20(7):898-912
references.pmid: 16600915
references.citation: Nature. 2000 Apr 20;404(6780):889-92
references.pmid: 10786797