title: Inducible-expression plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans.
abstract: We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.
authors.forename: Alice C
authors.initials: AC
authors.lastname: Ind
authors.forename: Steven L
authors.initials: SL
authors.lastname: Porter
authors.forename: Mostyn T
authors.initials: MT
authors.lastname: Brown
authors.forename: Elaine D
authors.initials: ED
authors.lastname: Byles
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: de Beyer
authors.forename: Scott A
authors.initials: SA
authors.lastname: Godfrey
authors.forename: Judith P
authors.initials: JP
authors.lastname: Armitage
mesh_terms: D002874:Chromosome Mapping; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial / Q000187:drug effects; D005822:Genetic Vectors; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007613:Kanamycin Resistance / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D010231:Paracoccus denitrificans / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012093:Replicon; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000235:genetics*
chemical_list: D007544:Isopropyl Thiogalactoside
references.citation: Biomol Eng. 2000 Oct;17(1):11-6
references.pmid: 11042472
references.citation: Methods Enzymol. 2007;423:392-413
references.pmid: 17609142
references.citation: Biol Chem. 2008 Jan;389(1):69-78
references.pmid: 18095871
references.citation: Appl Environ Microbiol. 1998 Jun;64(6):2240-6
references.pmid: 9603842
references.citation: J Biol Chem. 2006 Oct 27;281(43):32694-704
references.pmid: 16950782
references.citation: J Bacteriol. 1994 Jun;176(12):3692-7
references.pmid: 8206847
references.citation: J Bacteriol. 2008 Jan;190(1):286-99
references.pmid: 17965166
references.citation: EMBO J. 2000 Sep 1;19(17):4601-13
references.pmid: 10970853
references.citation: Appl Environ Microbiol. 2003 Feb;69(2):725-33
references.pmid: 12570988
references.citation: Trends Microbiol. 2008 Jun;16(6):251-60
references.pmid: 18440816
references.citation: J Immunol Methods. 1995 Mar 27;180(2):213-8
references.pmid: 7714335
references.citation: J Bacteriol. 1999 Jan;181(1):100-6
references.pmid: 9864318
references.citation: Gene. 1988 Oct 15;70(1):191-7
references.pmid: 2853689