title: Proteomic analysis of cold stress-responsive proteins in Thellungiella rosette leaves.
abstract: Low temperature is one of the most severe environmental factors that impair plant growth and agricultural production. To investigate how Thellungiella halophila, an Arabidopsis-like extremophile, adapts to cold stress, a comparative proteomic approach based on two-dimensional electrophoresis was adopted to identify proteins that changed in abundance in Thellungiella rosette leaves during short term (6 h, 2 and 5 days) and long term (24 days) exposure to cold stress. Sixty-six protein spots exhibited significant change at least at one time point and maximal cold stress induced-proteome change was found in long-term cold stress group while the minimal change was found in 6-h cold treatment group. Fifty protein spots were identified by mass spectrometry analysis. The identified proteins mainly participate in photosynthesis, RNA metabolism, defense response, energy pathway, protein synthesis, folding and degradation, cell wall and cytoskeleton and signal transduction. These proteins might work cooperatively to establish a new homeostasis under cold stress. Nearly half of the identified cold-responsive proteins were associated with various aspects of chloroplast physiology suggesting that the cold stress tolerance of T. halophila is achieved, at least partly, by regulation of chloroplast function. All protein spots involved in RNA metabolism, defense response, protein synthesis, folding and degradation were found to be upregulated markedly by cold treatment, indicating enhanced RNA metabolism, defense and protein metabolism may play crucial roles in cold tolerance mechanism in T. halophila.
authors.affiliation: Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, 100875 Beijing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.forename: Liumin
authors.initials: L
authors.lastname: Fan
authors.forename: Genfa
authors.initials: G
authors.lastname: Zhang
mesh_terms: D019607:Brassicaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003080:Cold Temperature; D004573:Electrolytes / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D013997:Time Factors
chemical_list: D004573:Electrolytes; D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger
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