Proteomics of proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under the influence of sodium orthovanadate.
An extensive proteomics analysis has identified proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under oxidative stress induced by sodium orthovanadate (SOV). Measurement of total carotenoid accumulation per cell biomass showed an increase from 81 to 136 pg/cell after being exposed to 2.5 mM SOV, when compared to the control cells at day 3 of cultivation. A total of 83 proteins were differentially expressed in SOV-treated H. lacustris in comparison with control cells. They consisted of 34 down-regulated and 49 up-regulated proteins. Of these, 17 highly-expressed proteins were analyzed by MALDI-TOF-MS to identify the function of the differentially expressed proteins in response to oxidative stress in H. lacustris.
Tran
Ngoc-Phuong
NP
Department of Biological Engineering, Institute of Industrial Biotechnology, Inha University, In-Cheon, 402-751, Korea.
Park
Jae-Kweon
JK
Hong
Seong-Joo
SJ
Lee
Choul-Gyun
CG
D020418:Algal Proteins / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis; D015536:Down-Regulation; D020869:Gene Expression Profiling; D016877:Oxidants / Q000633:toxicity*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation; D014638:Vanadates / Q000633:toxicity*; D016823:Volvocida / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D024341:Xanthophylls / Q000378:metabolism
D020418:Algal Proteins; D016877:Oxidants; D020543:Proteome; D024341:Xanthophylls; D002338:Carotenoids; D014638:Vanadates; C005948:astaxanthine