An Mrp-like cluster in the halotolerant cyanobacterium Aphanothece halophytica functions as a Na+/H+ antiporter.
The mrp homolog gene cluster mrpCD1D2EFGAB (Ap-mrp) was found in a halotolerant cyanobacterium, Aphanothece halophytica, amplified, and expressed in Escherichia coli mutant TO114. Ap-mrp complemented the salt-sensitive phenotype of TO114 and exhibited Na(+)/H(+) and Li(+)/H(+) exchange activities, indicating that Ap-Mrp functions as a Na(+)/H(+) antiporter.
Fukaya
Fuminori
F
Graduate School of Environmental and Human Sciences, Meijo University, Nagoya 468-8502, Japan.
Promden
Worrawat
W
Hibino
Takashi
T
Tanaka
Yoshito
Y
Nakamura
Tatsunosuke
T
Takabe
Teruhiro
T
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism*; D046940:Synechococcus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D017923:Sodium-Hydrogen Exchangers
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