title: Titanate particles as agents to deliver gold compounds to fibroblasts and monocytes.
abstract: Titanates are inorganic compounds with high affinity for specific metal ions or metal compounds, including gold. We have previously demonstrated that both monosodium titanate (MST) and amorphous peroxo-titanate (APT) alone do not suppress cellular metabolism of several cell types, and we have shown that MST and APT adsorb and release gold compounds in biological salt solutions. In the current study, we extend this work and show that MST and APT loaded with two gold compounds deliver sufficient levels of these compounds to alter the metabolism of mammalian cells. Fibroblasts (L929) or monocytes (THP1) were exposed to MST and APT loaded with either Au(III) or Auranofin(R), a Au(I)-organic compound, for 24-72 h, after which succinate dehydrogenase (SDH) activity of the cells was measured using the MTT method. MST or APT alone did not suppress SDH activity of either cell type. AF and Au(III) alone suppressed SDH activity completely above 2 muM or 300 muM, respectively. APT and MST loaded with either gold compound suppressed L929 fibroblast SDH activity by 30-80% after 72 h, but Au(III)-loaded APT was more potent than AF-loaded APT. Monocyte SDH activity was not affected by any loaded titanate. Our results suggest that titanates could be used for solid phase delivery of metal compounds to affect mammalian cell function of some types of cells.
authors.affiliation: Department of Oral Biology, Medical College of Georgia, Augusta, Georgia, USA.
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Davis
authors.forename: David T
authors.initials: DT
authors.lastname: Hobbs
authors.forename: Rania
authors.initials: R
authors.lastname: Khashaba
authors.forename: Poojitha
authors.initials: P
authors.lastname: Sehkar
authors.forename: Francesca N
authors.initials: FN
authors.lastname: Seta
authors.forename: Regina L W
authors.initials: RL
authors.lastname: Messer
authors.forename: Jill B
authors.initials: JB
authors.lastname: Lewis
authors.forename: John C
authors.initials: JC
authors.lastname: Wataha
mesh_terms: D000818:Animals; D001310:Auranofin / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D017612:Gold Compounds / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*
chemical_list: D017612:Gold Compounds; D010087:Oxides; C525208:peroxo-titanate; C471701:sodium titanate; D001310:Auranofin; D014025:Titanium