Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella.
A pale-yellowish-pigmented strain, 022-2-26(T), was isolated from a starfish, Stellaster equestris. Cells of strain 022-2-26(T) were Gram-negative short rods that were chemo-organotrophic, alkalitolerant and mesophilic. The predominant menaquinone was MK-6. The major cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1), C(15 : 0), iso-C(15 : 0) 2-OH and iso-C(17 : 0) 3-OH (together representing 87 % of the total fatty acids). The DNA G+C content was 30.1 mol%. A 16S rRNA gene sequence of the isolate was determined and phylogenetic analyses revealed that strain 022-2-26(T) formed a robust clade (neighbour-joining algorithm with a bootstrap value of 95 % and parsimony and maximum-likelihood algorithms) with type strains of species in the genus Winogradskyella. The closest phylogenetic neighbour of strain 022-2-26(T) was Winogradskyella poriferorum UST030701-295(T) (96 % 16S rRNA gene sequence similarity; 59 differences between sequences). On the basis of the phenotypic and chemotaxonomic characteristics and the phylogenetic evidence, it is proposed that strain 022-2-26(T) represents a novel species, Winogradskyella exilis sp. nov. The type strain is 022-2-26(T) (=KMM 6013(T) =CIP 109976(T)).
Ivanova
Elena P
EP
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russian Federation.|Swinburne University of Technology, Faculty of Life and Social Sciences, PO Box 218, Hawthorn, Victoria 3122, Australia.
Christen
Richard
R
CNRS, UMR 6543, Laboratoire de Biologie Virtuelle, Parc Valrose, F-06108 Nice, France.
Gorshkova
Nataliya M
NM
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russian Federation.
Zhukova
Natalia V
NV
Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041 Vladivostok, Russian Federation.
Kurilenko
Valeriya V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russian Federation.
Crawford
Russell J
RJ
Swinburne University of Technology, Faculty of Life and Social Sciences, PO Box 218, Hawthorn, Victoria 3122, Australia.
Mikhailov
Valery V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russian Federation.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013215:Starfish / Q000382:microbiology*; D024482:Vitamin K 2 / Q000032:analysis
D008055:Lipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2