The salicylic acid-induced protection of non-climacteric unripe pepper fruit against Colletotrichum gloeosporioides is similar to the resistance of ripe fruit.
The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment protects unripe pepper fruit against the fungus, while jasmonic acid (JA) does not. The SA-mediated LAR in the unripe fruit inhibited the fungal appressoria, resulting in protection against fungal infection. Microarray analysis revealed that 177 of 7,900 cDNA clones showed more than fourfold transcriptional accumulation in SA-treated unripe fruit. The reverse transcription-polymerase chain reaction showed that most of the SA-responsive genes (SRGs) were regulated by SA, but not by JA or ethylene-releasing ethephon. Furthermore, most of the SRGs were preferentially expressed in the ripe fruit. These results suggest that the SA-mediated transcriptional regulation of SRGs has a critical role in the resistance of ripe pepper fruit to fungal infection.
Lee
Sanghyeob
S
Bio-application Team, Dongbu Advanced Research Institute, 103-2 Munji-dong, Daeduck Science Town, Daejeon 305-708, Korea.
Hong
Jong-Chan
JC
Jeon
Woong Bae
WB
Chung
Young-Soo
YS
Sung
Soonkee
S
Choi
Doil
D
Joung
Young Hee
YH
Oh
Boung-Jun
BJ
D002212:Capsicum / Q000235:genetics* / Q000382:microbiology; D020231:Colletotrichum / Q000187:drug effects*; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D005638:Fruit / Q000235:genetics* / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D007539:Isonicotinic Acids / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018749:RNA, Plant / Q000235:genetics; D020156:Salicylic Acid / Q000494:pharmacology*; D013830:Thiadiazoles / Q000494:pharmacology
D003517:Cyclopentanes; D018076:DNA, Complementary; D007539:Isonicotinic Acids; D054883:Oxylipins; D018749:RNA, Plant; D013830:Thiadiazoles; C077261:2,6-dichloroisonicotinic acid; C011006:jasmonic acid; C099403:S-methyl benzo(1,2,3)thiadiazole-7-carbothioate; D020156:Salicylic Acid
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