A microfluidic platform for studying the effects of small temperature gradients in an incubator environment.
Studies on the effects of variations in temperature and mild temperature gradients on cells, gels, and scaffolds are important from the viewpoint of biological function. Small differences in temperature are known to elicit significant variations in cell behavior and individual protein reactivity. For the study of thermal effects and gradients in vitro, it is important to develop microfluidic platforms which are capable of controlling temperature gradients in an environment which mimics the range of physiological conditions. In the present paper, such a microfluidic thermal gradient system (muTGS) system is proposed which can create and maintain a thermal gradient throughout a cell-seeded gel matrix using the hot and cold water supply integrated in the system in the form of a countercurrent heat exchanger. It is found that a uniform temperature gradient can be created and maintained in the device even inside a high temperature and high humidity environment of an incubator. With the help of a hot and cold circuit controlled from outside the incubator the temperature gradient can be regulated. A numerical simulation of the device demonstrates the thermal feature of the chip. Cell viability and activity under a thermal gradient are examined by placing human breast cancer cells in the device.
Das
Sarit K
SK
Chung
Seok
S
Zervantonakis
Ioannis
I
Atnafu
Joseph
J
Kamm
Roger D
RD
Lab Chip. 2003 May;3(2):100-5
15100790
J Neurosci. 2002 Jul 1;22(13):5727-33
12097525
Anal Chem. 2003 Jan 1;75(1):1-7
12530811
Nat Biotechnol. 2002 Aug;20(8):826-30
12091913
J Am Chem Soc. 2002 Apr 24;124(16):4432-5
11960472
J Am Chem Soc. 2003 Sep 17;125(37):11170-1
16220918
Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16531-6
12486223
Nat Cell Biol. 2000 Aug;2(8):469-75
10934466
Science. 1998 Jun 12;280(5370):1735-7
9624047
Electrophoresis. 1996 Jul;17(7):1195-201
8855404
Nature. 1995 Jul 27;376(6538):344-8
7630402
Electrophoresis. 2002 Feb;23(4):596-601
11870771
Science. 2000 Sep 8;289(5485):1760-3
10976071
J Forensic Sci. 1998 Mar;43(2):315-9
9544539
Nat Cell Biol. 2005 Oct;7(10):947-53
16179950
J Cell Sci. 1991 Aug;99 ( Pt 4):769-75
1770004
Science. 2005 Nov 18;310(5751):1139-43
16293750
Science. 1991 Feb 15;251(4995):767-73
1990438
Anal Chem. 2004 Sep 15;76(18):5257-64
15362881
Science. 1997 May 30;276(5317):1425-8
9162012
Nat Med. 2003 Feb;9(2):149-50
12563318
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15757-61
16236724
J Neurosci. 1994 Jan;14(1):29-38
8283236
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4061-5
1060088
Nature. 2006 Jul 27;442(7101):403-11
16871208
Angew Chem Int Ed Engl. 2004 May 3;43(19):2508-11
15127437
Biotechnol Adv. 2006 May-Jun;24(3):243-84
16326063
Nature. 2006 Jul 27;442(7101):368-73
16871203
Science. 1998 Apr 10;280(5361):267-70
9535652
Nature. 2005 Apr 28;434(7037):1134-8
15858575
Cell Motil Cytoskeleton. 2005 Jan;60(1):24-34
15573414
Methods Mol Biol. 2005;294:347-57
15576923
Anal Chem. 1999 Aug 1;71(15):3258-64
10450166
Anal Chem. 2006 Dec 1;78(23):8028-35
17134136
Int J Hyperthermia. 2008 Feb;24(1):41-56
18214768
Anal Biochem. 2000 Jan 1;277(1):157-60
10610701