High prevalence of plasmid-mediated quinolone resistance determinants in commensal members of the Enterobacteriaceae in Ho Chi Minh City, Vietnam.
Antimicrobial-resistant pathogenic members of the Enterobacteriaceae are a well-defined global problem. We hypothesized that one of the main reservoirs of dissemination of antimicrobial resistance genes in Vietnam is non-pathogenic intestinal flora, and sought to isolate antimicrobial-resistant organisms from hospitalized patients and non-hospitalized healthy individuals in Ho Chi Minh City. The results identified substantial faecal carriage of gentamicin-, ceftazidime- and nalidixic acid-resistant members of the Enterobacteriaceae in both hospitalized patients and non-hospitalized healthy individuals. A high prevalence of quinolone resistance determinants was identified, particularly the qnrS gene, in both community- and hospital-associated strains. Furthermore, the results demonstrated that a combination of quinolone resistance determinants can confer resistance to nalidixic acid and ciprofloxacin, even in the apparent absence of additional chromosomal resistance mutations in wild-type strains and laboratory strains with transferred plasmids. These data suggest that intestinal commensal organisms are a significant reservoir for the dissemination of plasmid-mediated quinolone resistance in Ho Chi Minh City.
Minh Vien
Le Thi
LT
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Baker
Stephen
S
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Phuong Thao
Le Thi
LT
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Phuong Tu
Le Thi
LT
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Thu Thuy
Cao
C
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Thu Nga
Tran Thi
TT
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Minh Hoang
Nguyen Van
NV
Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.|Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Campbell
James Iain
JI
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Minh Yen
Lam
L
Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Trong Hieu
Nguyen
N
Hung Vuong Hospital, Ho Chi Minh City, Vietnam.
Vinh Chau
Nguyen Van
NV
Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Farrar
Jeremy
J
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Schultsz
Constance
C
Center for Poverty-related Communicable Diseases (CPCD), Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.|Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002353:Carrier State; D002648:Child; D002675:Child, Preschool; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006801:Humans; D007223:Infant; D009154:Mutation; D010957:Plasmids / Q000235:genetics*; D015363:Quinolones / Q000494:pharmacology*; D014744:Vietnam / Q000453:epidemiology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015363:Quinolones
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