Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection.
OBJECTIVE
We have previously shown that a transcriptome is found in saliva and subpanels of these mRNAs can be used as oral cancer biomarkers. In this study, we measured the presence of microRNAs (miRNA) in saliva and determined their potential as an additional set of oral cancer biomarkers.


METHODS
A total of 314 miRNAs were measured using reverse transcriptase-preamplification-quantitative PCR in 12 healthy controls. Degradation pattern of endogenous and exogenous saliva miRNAs were measured at room temperature over time. Selected miRNAs were validated in saliva of 50 oral squamous cell carcinoma patients and 50 healthy matched control subjects.


RESULTS
We detected approximately 50 miRNAs in both the whole and supernatant saliva. Endogenous saliva miRNA degraded much slower compared with exogenous miRNA. Two miRNAs, miR-125a and miR-200a, were present in significantly lower levels (P < 0.05) in the saliva of oral squamous cell carcinoma patients than in control subjects.


CONCLUSIONS
Both whole and supernatant saliva of healthy controls contained dozens of miRNAs, and similar to saliva mRNAs, these miRNAs are stable. Saliva miRNAs can be used for oral cancer detection.
Park
Noh Jin
NJ
Dental Research Institute, Serbia.
Zhou
Hui
H
Elashoff
David
D
Henson
Bradley S
BS
Kastratovic
Dragana A
DA
Abemayor
Elliot
E
Wong
David T
DT
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D009055:Mouth / Q000378:metabolism; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*
D014408:Biomarkers, Tumor; D035683:MicroRNAs
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