Adverse effects of cadmium exposure on mouse sperm.
The effects of cadmium chloride exposure on sperm functional parameters were evaluated on eight-week-old ICR-CD1 male mice administered with a single s.c. injection of 1, 2 and 3 mg CdCl(2)/kg bw. Groups of animals treated with each dose, as well as their respective controls, were sacrificed after 24h to detect short-term (acute) effects and after 35 days. Sperm cells were collected from the epididymis and several parameters of sperm quality and function were evaluated, namely density, morphology, motility, viability, mitochondrial function, acrosome integrity, together with DNA fragmentation assessed by the TUNEL assay. The short-term effects of cadmium chloride resulted in an increased fraction of sperm with abnormal morphology, premature acrosome reaction and reduced motility. Late term effects (after 35 days) included a drastic reduction of sperm cell numbers and sperm motility. An increase in DNA fragmentation was also detected.
Oliveira
Helena
H
Department of Biology, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal. holiveira@ua.pt
Spanò
Marcello
M
Santos
Conceição
C
Pereira
Maria de Lourdes
Mde L
D020101:Acrosome Reaction / Q000187:drug effects*; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D004822:Epididymis / Q000166:cytology; D020287:In Situ Nick-End Labeling; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D023382:Toxicity Tests, Acute
D004785:Environmental Pollutants; D002104:Cadmium