Interaction trap/two-hybrid system to identify interacting proteins.
The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.
Golemis
Erica A
EA
Fox Chase Cancer Center, Philadelphia, Pennsylvania.
Serebriiskii
Ilya
I
Fox Chase Cancer Center, Philadelphia, Pennsylvania.
Finley
Russell L
RL
Wayne State University School of Medicine, Detroit, Michigan.
Kolonin Hunt By Interaction Mating
Mikhail G
MG
Wayne State University School of Medicine, Detroit, Michigan.
Gyuris
Jeno
J
Mitotix, Inc, Cambridge, Massachusetts.
Brent
Roger
R
The Molecular Sciences Institute, Berkeley, California.
D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D010957:Plasmids; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
D011506:Proteins; D011993:Recombinant Fusion Proteins