Detection of Coccidioides antigenemia following dissociation of immune complexes.
Having reported that pretreatment of serum samples with EDTA at 100 degrees C improved the sensitivity for the detection of Histoplasma antigenemia, we have evaluated this method for the detection of Coccidioides antigenemia. Urine and serum samples from patients with coccidioidomycosis were tested using the MVista Coccidioides enzyme immunoassay, and serum samples with and without EDTA-heat treatment were tested. Antigenemia was detected in 28.6% of patients whose samples were not EDTA-heat treated and in 73.1% of those whose samples were treated. Antigenuria was detected in 50% of patients. Specificity of 100% was obtained in healthy subjects, but cross-reactions were seen in 22.2% of patients with histoplasmosis or blastomycosis. EDTA-heat treatment improves the sensitivity for the detection of Coccidioides antigenemia.
Durkin
Michelle
M
MiraVista Diagnostics, Indianapolis, Indiana1; MiraBella Technologies, Indianapolis, Indiana 46241, USA.
Estok
Lois
L
Hospenthal
Duane
D
Crum-Cianflone
Nancy
N
Swartzentruber
Samantha
S
Hackett
Emily
E
Wheat
L Joseph
LJ
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D000936:Antigen-Antibody Complex / Q000097:blood; D000946:Antigens, Fungal / Q000097:blood*; D001759:Blastomycosis / Q000175:diagnosis / Q000276:immunology; D016022:Case-Control Studies; D003045:Coccidioides / Q000276:immunology*; D003047:Coccidioidomycosis / Q000150:complications / Q000175:diagnosis* / Q000276:immunology* / Q000382:microbiology; D003429:Cross Reactions; D004492:Edetic Acid; D006660:Histoplasmosis / Q000175:diagnosis / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D000936:Antigen-Antibody Complex; D000946:Antigens, Fungal; D004492:Edetic Acid
J Clin Microbiol. 2004 Oct;42(10):4873-5
15472368
J Clin Microbiol. 1995 Mar;33(3):618-9
7751365
Clin Chest Med. 2009 Jun;30(2):379-89, viii
19375642
Clin Vaccine Immunol. 2007 Dec;14(12):1587-91
17913863
J Clin Microbiol. 1983 Jul;18(1):136-42
6885984
Clin Vaccine Immunol. 2009 Mar;16(3):320-2
19144790
Am J Trop Med Hyg. 1984 Jul;33(4):645-9
6476210
Clin Infect Dis. 2008 Oct 15;47(8):e69-73
18781884
J Infect Dis. 1991 Mar;163(3):671-4
1899877