Enterorhabdus caecimuris sp. nov., a member of the family Coriobacteriaceae isolated from a mouse model of spontaneous colitis, and emended description of the genus Enterorhabdus Clavel et al. 2009.
The C3H/HeJBir mouse model of intestinal inflammation was used for isolation of a Gram-positive, rod-shaped, non-spore-forming bacterium (B7(T)) from caecal suspensions. On the basis of partial 16S rRNA gene sequence analysis, strain B7(T) was a member of the class Actinobacteria, family Coriobacteriaceae, and was related closely to Enterorhabdus mucosicola Mt1B8(T) (97.6 %). The major fatty acid of strain B7(T) was C(16 : 0) (19.1 %) and the respiratory quinones were mono- and dimethylated. Cells were aerotolerant, but grew only under anoxic conditions. Strain B7(T) did not convert the isoflavone daidzein and was resistant to cefotaxime. The results of DNA-DNA hybridization experiments and additional physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain B7(T) from the type strain of E. mucosicola. Therefore, strain B7(T) represents a novel species, for which the name Enterorhabdus caecimuris sp. nov. is proposed. The type strain is B7(T) (=DSM 21839(T) =CCUG 56815(T)).
Clavel
Thomas
T
Biofunctionality, ZIEL - Research Center for Nutrition and Food Science, Technische Universität München (TUM), 85350 Freising Weihenstephan, Germany.
Duck
Wayne
W
Division of Gastroenterology and Hepatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Charrier
Cédric
C
NovaBiotics Ltd, Aberdeen, AB21 9TR, UK.
Wenning
Mareike
M
Microbiology, ZIEL - Research Center for Nutrition and Food Science, TUM, 85350 Freising Weihenstephan, Germany.
Elson
Charles
C
Division of Gastroenterology and Hepatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Haller
Dirk
D
Biofunctionality, ZIEL - Research Center for Nutrition and Food Science, Technische Universität München (TUM), 85350 Freising Weihenstephan, Germany.
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003092:Colitis / Q000382:microbiology*; D008055:Lipids / Q000032:analysis; D051379:Mice; D008809:Mice, Inbred C3H; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared
D008055:Lipids; D012336:RNA, Ribosomal, 16S
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