title: Protein-protein interactions of tandem affinity purified protein kinases from rice.
abstract: Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAP-tagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 TAP-tagged rice protein kinases with a concurrent screen using yeast two hybrid methods identified an evolutionarily new rice protein that interacts with the well conserved cell division cycle 2 (CDC2) protein complex.
authors.affiliation: Plant Science Initiative, University of Nebraska, Lincoln, NE, USA. jai.rohila@sdstate.edu
authors.forename: Jai S
authors.initials: JS
authors.lastname: Rohila
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.forename: Johann
authors.initials: J
authors.lastname: Chen
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Cerny
authors.forename: Christopher
authors.initials: C
authors.lastname: Dardick
authors.forename: Patrick
authors.initials: P
authors.lastname: Canlas
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Fujii
authors.forename: Michael
authors.initials: M
authors.lastname: Gribskov
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Kanrar
authors.forename: Lucas
authors.initials: L
authors.lastname: Knoflicek
authors.forename: Becky
authors.initials: B
authors.lastname: Stevenson
authors.forename: Mingtang
authors.initials: M
authors.lastname: Xie
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.forename: Xianwu
authors.initials: X
authors.lastname: Zheng
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.forename: Pamela
authors.initials: P
authors.lastname: Ronald
authors.forename: Michael E
authors.initials: ME
authors.lastname: Fromm
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D002846:Chromatography, Affinity; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010766:Phosphorylation; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D011494:Protein Kinases
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