Analysis on cDNA sequence, mRNA expression and imprinting status of Dlk1 gene in goats.
Dlk1 (Delta-like homolog 1) is a cell surface transmembrane glycoprotein belonging to the epidermal growth factor like family of homeotic proteins and plays an important role in regulating fetal and postnatal development. Increased expression of Dlk1 is the primary cause of muscle hypertrophy in the callipyge sheep exhibiting overgrowth of fast-twitch muscles and reduced adiposity. However, the function of Dlk1 in goats remains unknown. In this study, a fragment of 864 bp of goat Dlk1 cDNA, encoding 287 amino acids, was amplified, which has a high homology both in nucleotide sequence and amino acid sequence with the corresponding region of pig, cattle and sheep Dlk1. The Dlk1 was found to be expressed in most tissues of goat fetuses, and in the adrenal gland, pancreas and thymus of adult goats. Two alternative transcripts of Dlk1-C and Dlk1-C2 were expressed in both fetuses and adult goats. One C/T transition in the coding region of goat Dlk1 was identified and by genotyping one segregating goat family and the expressed allele in the tissues of the offspring, Dlk1 was found to be paternally expressed.
Cao
Guiling
G
Laboratory of Animal Molecular Genetics, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, Taian, China.
Zhang
Yujie
Y
Wang
Jianmin
J
Jiang
Yunliang
Y
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D018392:Genomic Imprinting / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger
Curr Biol. 2004 Oct 26;14(20):1858-62
15498495
Leukemia. 2005 Aug;19(8):1404-10
15959531
Exp Neurol. 2007 Apr;204(2):791-801
17320866
Anim Genet. 2008 Apr;39(2):189-92
18279496
Biochem Biophys Res Commun. 1999 Nov 2;264(3):662-7
10543989
Comp Biochem Physiol B Biochem Mol Biol. 2003 Feb;134(2):315-33
12568810
Mol Cell Endocrinol. 2007 Jun 15;271(1-2):55-63
17485162
Curr Biol. 2000 Sep 21;10(18):1135-8
10996796
Mol Cell Biol. 1997 Feb;17(2):977-88
9001251
Trends Genet. 2008 Jun;24(6):306-16
18471925
Differentiation. 2008 Mar;76(3):283-98
17697128
Anim Genet. 2006 Dec;37(6):535-42
17121598
Comp Biochem Physiol B Biochem Mol Biol. 2006 Sep;145(1):50-9
16901742
Biochem Biophys Res Commun. 2006 Feb 17;340(3):995-1005
16403460
Mamm Genome. 2006 Feb;17(2):157-67
16465595