Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods.
The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.
Mota
Crystiane Rodrigues Araújo
CR
Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia, GO, Brazil.
Miranda
Karla Carvalho
KC
Lemos
Janine de Aquino
Jde A
Costa
Carolina Rodrigues
CR
Hasimoto e Souza
Lúcia Kioko
LK
Passos
Xisto Sena
XS
Meneses e Silva
Hildene
H
Silva
Maria do Rosário Rodrigues
Mdo R
D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000145:classification / Q000187:drug effects*; D006801:Humans; D021261:Parasitic Sensitivity Tests / Q000379:methods
D000935:Antifungal Agents