title: The age-dependent induction of apoptosis-inducing factor (AIF) in the human semitendinosus skeletal muscle.
abstract: To assess the dependence on age of the expression of apoptosis regulatory proteins in the human semitendinosus muscle, we measured the expression levels of several apoptosis-related genes, including apoptosis-inducing factor (AIF), Bax, Bcl-2, caspase-3 and heat shock protein 70 (HSP70), using RT-PCR, immunohistochemistry and TUNEL assays. We found that the DNA fragmentation was proportional to the age of the tissues sample donors. The expression levels of AIF were significantly elevated (by 10 to 25%) in semitendinosus tissue samples from older individuals, but the Bax, Bcl-2, caspase-3 and HSP 70 levels remained almost constant. This data suggests that the morphological and functional changes observed in aged human semitendinosus muscle correlates with the apoptosis of muscle cells through the induction of AIF.
authors.affiliation: Sports Medicine Center, East-West Neo Medicial Center, Kyung Hee University, Seoul, 130-701, Korea. comskp@khu.ac.kr
authors.forename: Soo Yeon
authors.initials: SY
authors.lastname: Park
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyoung Ho
authors.initials: KH
authors.lastname: Yoon
authors.forename: Mun Seog
authors.initials: MS
authors.lastname: Chang
authors.forename: Seong Kyu
authors.initials: SK
authors.lastname: Park
mesh_terms: D000328:Adult; D000367:Age Factors; D017209:Apoptosis; D051033:Apoptosis Inducing Factor / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002648:Child; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D051033:Apoptosis Inducing Factor; D018840:HSP70 Heat-Shock Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D053148:Caspase 3
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