Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice.
This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DGCLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism.
Hue
Jin-Joo
JJ
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
Lee
Ki-Nam
KN
Jeong
Jae-Hwang
JH
Lee
Sang-Hwa
SH
Lee
Young Ho
YH
Jeong
Seong-woon
SW
Nam
Sang Yoon
SY
Yun
Young Won
YW
Lee
Beom Jun
BJ
D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects*; D004075:Diglycerides / Q000494:pharmacology*; D004195:Disease Models, Animal; D004435:Eating / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism* / Q000473:pathology; D047495:PPAR gamma / Q000378:metabolism; D013997:Time Factors
D019440:Anti-Obesity Agents; D004075:Diglycerides; D044243:Linoleic Acids, Conjugated; D008055:Lipids; D047495:PPAR gamma
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