A new liquid chromatography-tandem mass spectrometry method for determination of bisoprolol in human plasma samples.
Liquid chromatography (LC) coupled with mass spectrometry (MS) detection is one of the most powerful analytical tools for organic compound analysis. The advantages of using LC/MS methods over HPLC methods include: selectivity, chromatographic integrity, peak assignment, structural information, and rapid method development. In this paper, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of bisoprolol in human plasma samples, using metoprolol as internal standard and liquid-liquid extraction procedure. The assay has proven to be sensitive, specific and reproducible, suitable to determine the bisoprolol concentration, following a single oral administration of a 10 mg bisoprolol tablet in 22 healthy volunteers, in the bioequivalence study of Bisoprolol 10 mg coated tablets, produced by Antibiotice S.A. versus Concor 10 mg, produced by Merck.
Peste
Gabriela
G
Center for Drug Evaluation, Antibiotice S.A., Iasi, Romania. gabrielapeste@yahoo.com
Bibire
Nela
N
Apostu
Mihai
M
Vlase
Aurel
A
Oniscu
Corneliu
C
D017298:Bisoprolol / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013810:Therapeutic Equivalency
D017298:Bisoprolol
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