Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1H-NMR micro-flow probe.
A standard operating procedure (SOP) is presented for high-throughput metabonomics studies using a 600 MHz NMR spectrometer equipped with a micro-flow probe that is connected to an auto-sampler. The procedure is designed to minimize random and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the data acquired by a given NMR spectroscopist to ensure that (i) all researchers involved in the NMR data acquisition of a metabonomics research program perform equally and (ii) operator-associated variation of NMR data collection is statistically not relevant for the interpretation of results obtained from multivariate data analyses.
Sukumaran
Dinesh K
DK
Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY, USA.
Garcia
Erwin
E
Hua
Jia
J
Tabaczynski
Walter
W
Odunsi
Kunle
K
Andrews
Chris
C
Szyperski
Thomas
T
D019411:Clinical Laboratory Techniques / Q000295:instrumentation* / Q000592:standards*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D008832:Microchemistry; D015999:Multivariate Analysis; D044967:Serum / Q000737:chemistry*