Immunohistochemical expression of YKL-40 in peritoneal endometriosis.
OBJECTIVE
To evaluate the relationship between the immunohistochemical expression of YKL-40 and peritoneal endometriosis by using paraffinized peritoneal tissue blocks.


METHODS
We retrospectively collected data from 27 patients whose pathologic reports indicated invasion of the peritoneum by endometriosis. A conventional peroxidase staining technique was performed using rabbit polyclonal antibody (Quidel corporation, Santa Clara, CA) on peritoneal tissue blocks; their histology was then reviewed by a pathologist, and data were analyzed by nonparametric and Mann-Whitney tests.


RESULTS
YKL-40 was detected immunohistochemically in 17 (63%) of 27 cases. The number of biopsies which were invaded by endometriosis (P = 0.015), with a score based on the revised classification of endometriosis (American Society for Reproductive Medicine) had statistical significance (P = 0.001). The obstetric history, age, body mass index, severity, and occurrence of dysmenorrhea, menstrual phase, preoperative CA 125, erythrocyte sedimentation rate, and white blood cell count had no statistical significance. YKL-40 immunoreactivity was recognized as brown staining, localized to the cytoplasm of epithelial cells of endometrial gland in peritoneal endometriosis. There was no positive staining on endometrial stromal cells or smooth muscle cells.


CONCLUSIONS
YKL-40 is related to severity of peritoneal endometriosis. However why a different expression level of immunohistochemical staining should occur is not known and needs further investigation.
Kim
Pan Jo
PJ
Department of Obstetrics and Gynecology, Kyungpook National University, Graduate School Of Medicine, Daegu, Korea.
Hong
Dae Gy
DG
Park
Ji Young
JY
Cho
Young Lae
YL
Park
Il Soo
IS
Lee
Yoon Soon
YS
D054392:Adipokines; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D000071451:Chitinase-3-Like Protein 1; D004715:Endometriosis / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008875:Middle Aged; D010532:Peritoneal Diseases / Q000378:metabolism*; D012189:Retrospective Studies; D012720:Severity of Illness Index; D014018:Tissue Distribution
D054392:Adipokines; D015415:Biomarkers; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D006023:Glycoproteins; D037102:Lectins