Poly(lactic-co-glycolic acid) electrospun fibrous meshes for the controlled release of retinoic acid.
Poly(lactic-co-glycolic acid) (PLGA) meshes loaded with retinoic acid (RA) were prepared by applying the electrospinning technique. The purpose of the present work was to combine the biological effects of RA and the advantages of electrospun meshes to enhancing the mass transfer features of controlled release systems and cell interaction with polymeric scaffolds. The processing conditions for the fabrication of three-dimensional meshes were optimized by studying their influence on mesh morphology. Tensile testing showed that RA loading influenced the meshes' mechanical properties by increasing their strength and rigidity. Moreover, the drug release and degradation profiles of the electrospun systems were compared to analogous RA-loaded PLGA films prepared by solvent casting. The results of this study highlight that the electrospun meshes preserved their fibrous structure after 4 months under in vitro physiological conditions and showed a sustained controlled release of the loaded agent in comparison to that observed for cast films. The bioactivity of the loaded RA was investigated on murine preosteoblasts cells by evaluating its influence on cell proliferation and morphology.
Puppi
Dario
D
Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab), Department of Chemistry and Industrial Chemistry, University of Pisa, Pisa, Italy.
Piras
Anna Maria
AM
Detta
Nicola
N
Dinucci
Dinuccio
D
Chiellini
Federica
F
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000378:metabolism*; D004560:Electricity; D006016:Glycolates / Q000737:chemistry* / Q000494:pharmacology; D019344:Lactic Acid; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013718:Tensile Strength / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014212:Tretinoin / Q000378:metabolism*
D003692:Delayed-Action Preparations; D006016:Glycolates; D012996:Solutions; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014212:Tretinoin