title: High-Q polymer-coated microspheres for immunosensing applications.
abstract: Homogeneous polymeric thin layers have been used as functionalizing agents on silica microspherical resonators in view of the implementation of an immunosensor. We have characterized the microspheres functionalized with poly-L-lactic acid and Eudragit L100, as an alternative to the commonly used 3-Aminopropyltrimethoxysilane. It is shown that polymeric functionalization does not affect the high quality factor (Q greater than 10(7)) of the silica microspheres, and that the Q factor is about 3 x 10(5) after chemical activation and covalent binding of immunogammaglobulin (IgG). This functionalizing process of the microresonator constitutes a promising step towards the achievement of an ultra sensitive immunosensor.
authors.affiliation: IFAC-CNR, NelloCarrara Institute of Applied Physics, via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence, Italy. s.soria@ifac.cnr.it
authors.forename: S
authors.initials: S
authors.lastname: Soria
authors.forename: F
authors.initials: F
authors.lastname: Baldini
authors.forename: S
authors.initials: S
authors.lastname: Berneschi
authors.forename: F
authors.initials: F
authors.lastname: Cosi
authors.forename: A
authors.initials: A
authors.lastname: Giannetti
authors.forename: G Nunzi
authors.initials: GN
authors.lastname: Conti
authors.forename: S
authors.initials: S
authors.lastname: Pelli
authors.forename: G C
authors.initials: GC
authors.lastname: Righini
authors.forename: B
authors.initials: B
authors.lastname: Tiribilli
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004867:Equipment Design; D006801:Humans; D007107:Immune System / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D008863:Microspheres; D055095:Optics and Photonics; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D011091:Polyesters; D011108:Polymers; D011109:Polymethacrylic Acids; C038300:methylmethacrylate-methacrylic acid copolymer; D019344:Lactic Acid; C033616:poly(lactide); D012822:Silicon Dioxide