title: Cell encapsules with tunable transport and mechanical properties.
abstract: We utilized a microfluidic device with hydrodynamic flow focusing geometry to produce uniform agarose droplets in the range of 50 to 110 mum. The transport property of the thermally gelled particles was tailored by layer-by-layer (LBL) polyelectrolytes coating on the surface and was measured via the release rates of Rhodamine B. The mechanical strength of the capsules was further enhanced by a coating of silica nano-particles in addition to polyelectrolyte coatings. We demonstrated that yeast cells can be successfully encapsulated into agarose capsules.
authors.affiliation: Artie McFerrin, Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122, USA and INEST (Interdisciplinary Network of Emerging Science and Technologies) Group Postgraduate Program, Philip Morris USA, Richmond, Virginia 23234, USA.
authors.forename: Dawei
authors.initials: D
authors.lastname: Luo
authors.forename: Srinivasa Rao
authors.initials: SR
authors.lastname: Pullela
authors.forename: Manuel
authors.initials: M
authors.lastname: Marquez
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Cheng
references.citation: J Control Release. 2000 May 15;66(2-3):281-92
references.pmid: 10742587
references.citation: Hum Gene Ther. 1993 Aug;4(4):433-40
references.pmid: 8399490
references.citation: Int J Pharm. 1999 Sep 30;187(1):115-23
references.pmid: 10502618
references.citation: Science. 1980 Nov 21;210(4472):908-10
references.pmid: 6776628
references.citation: Biomaterials. 2005 Jun;26(16):3327-31
references.pmid: 15603828
references.citation: Science. 2002 Aug 16;297(5584):1197-8
references.pmid: 12183634
references.citation: Trends Biotechnol. 1996 May;14(5):158-62
references.pmid: 8645450
references.citation: Transplant Proc. 1992 Jun;24(3):997
references.pmid: 1604705
references.citation: Biomicrofluidics. 2007 Jan 25;1(1):14105
references.pmid: 19693354
references.citation: Biomaterials. 2005 Aug;26(23):4786-92
references.pmid: 15763258
references.citation: Biotechnol Bioeng. 2004 Apr 20;86(2):168-73
references.pmid: 15052636
references.citation: Transplant Proc. 1992 Jun;24(3):952
references.pmid: 1604680
references.citation: Transplant Proc. 1992 Dec;24(6):2922-3
references.pmid: 1466001
references.citation: FASEB J. 2002 Feb;16(2):213-5
references.pmid: 11772948
references.citation: Nat Med. 2003 Jan;9(1):104-7
references.pmid: 12514721
references.citation: Angew Chem Int Ed Engl. 1998 Mar 16;37(5):550-575
references.pmid: 29711088
references.citation: J Microencapsul. 1987 Jul-Sep;4(3):239-42
references.pmid: 3332901
references.citation: Science. 1964 Oct 23;146(3643):524-5
references.pmid: 14190240
references.citation: Hum Gene Ther. 1996 Jan;7(1):61-70
references.pmid: 8825869
references.citation: Electrophoresis. 2005 Oct;26(19):3716-24
references.pmid: 16196106