Crystal structure of prunin-1, a major component of the almond (Prunus dulcis) allergen amandin.
Seed storage proteins are accumulated during seed development and act as a reserve of nutrition for seed germination and young sprout growth. Plant seeds play an important role in human nutrition by providing a relatively inexpensive source of protein. However, many plant foods contain allergenic proteins, and the number of people suffering from food allergies has increased rapidly in recent years. The 11S globulins are the most widespread seed storage proteins, present in monocotyledonous and dicotyledonous seeds as well as in gymnosperms (conifers) and other spermatophytes. This family of proteins accounts for a number of known major food allergens. They are of interest to both the public and industry due to food safety concerns. Because of the interests in the structural basis of the allergenicity of food allergens, we sought to determine the crystal structure of Pru1, the major component of the 11 S storage protein from almonds. The structure was refined to 2.4 A, and the R/Rfree for the final refined structure is 17.2/22.9. Pru1 is a hexamer made of two trimers. Most of the back-to-back trimer-trimer association was contributed by monomer-monomer interactions. An alpha helix (helix 6) at the C-terminal end of the acidic domain of one of the interacting monomers lies at the cleft of the two protomers. The residues in this helix correspond to a flexible region in the peanut allergen Ara h 3 that encompasses a previously defined linear IgE epitope.
Jin
Tengchuan
T
Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, 3101 South Dearborn Street, Chicago, Illinois 60616, USA.
Albillos
Silvia M
SM
Guo
Feng
F
Howard
Andrew
A
Fu
Tong-Jen
TJ
Kothary
Mahendra H
MH
Zhang
Yu-Zhu
YZ
D000485:Allergens / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005916:Globulins / Q000737:chemistry*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000276:immunology*; D010940:Plant Proteins / Q000737:chemistry*; D027861:Prunus / Q000276:immunology*; D012639:Seeds / Q000737:chemistry / Q000276:immunology*
D000485:Allergens; D005916:Globulins; D010455:Peptides; D010940:Plant Proteins; C463779:amandin; C039828:prunin protein, Prunus