title: Soft matter dispersions with ordered inner structures, stabilized by ethoxylated phytosterols.
abstract: This paper describes the formation and characterization of liquid crystalline dispersions based on the hexagonal phase of GMO/tricaprylin/water. As a stabilizer of the soft particles dispersed in the aqueous phase, a non-ionic, non-polymeric surfactant--ethoxylated phytosterol with 30 oxyethylene units (PhEO) was utilized. In contrast to Pluronic copolymers, normally utilized in the stabilization of liquid crystalline dispersions with ordered inner structure, use of such non-polymeric surfactant is not a common practice in this field. We revealed how properties of these particles, such as internal structure, size, and stability, can be rationally modified by the concentration of the stabilizing agent and processing conditions. The physical stability of the hexosomes was further examined by the LUMiFuge technique. Structural effect of PhEO solubilization on the properties of the bulk H(II) mesophase system showed that phase behavior was greatly influenced following phase transitions: H(II)-->H(II)+cubic-->cubic+L(alpha)-->L(alpha). The decrease of hydrogen bonding of the hydroxyl and carbonyl groups of monoolein with water and simultaneous hydration of EO groups of PhEO appeared to be important for the observed behavior. The use of PhEO as a dispersant resulted in a soft matter multi-phase water dispersion with bimodal distribution of the particle population. Effective stabilization of hexosomes was obtained in an extremely narrow concentration range of PhEO (0.1-0.2 wt%), coexisting with small vesicles and disordered particles. At higher PhEO content, particles had disordered inner structure, and unilamellar and multilamellar vesicles, at the expense of hexosomes in consequence of incorporation of the dispersant into the hexosome structure. PhEO was found to induce lamellar phase formation, introducing disorder into the hexagonal LLC and reducing their domain size. Finally, hexosomes were evaluated as delivery vehicles for the therapeutic peptide desmopressin. Sustained release of this drug was observed during the first 10 h; however, permeation drastically increased in the 10-24 h range.
authors.affiliation: Casali Institute of Applied Chemistry, The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Dima
authors.initials: D
authors.lastname: Libster
authors.forename: Abraham
authors.initials: A
authors.lastname: Aserin
authors.forename: Doron
authors.initials: D
authors.lastname: Yariv
authors.forename: Gil
authors.initials: G
authors.lastname: Shoham
authors.forename: Nissim
authors.initials: N
authors.lastname: Garti
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D003894:Deamino Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology; D005019:Ethyl Ethers / Q000737:chemistry*; D066298:In Vitro Techniques; D008027:Light; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D010539:Permeability / Q000187:drug effects; D010840:Phytosterols / Q000737:chemistry*; D053838:Scattering, Small Angle; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D005019:Ethyl Ethers; D010840:Phytosterols; D014867:Water; D003894:Deamino Arginine Vasopressin