Effects of an onion by-product on bioactivity and safety markers in healthy rats.
Onions are excellent sources of bioactive compounds including fructo-oligosaccharides (FOS) and polyphenols. An onion by-product was characterised in order to be developed as a potentially bioactive food ingredient. Our main aim was to investigate whether the potential health and safety effects of this onion by-product were shared by either of two derived fractions, an extract containing the onion FOS and polyphenols and a residue fraction containing mainly cell wall materials. We report here on the effects of feeding these products on markers of potential toxicity, protective enzymes and gut environment in healthy rats. Rats were fed during 4 weeks with a diet containing the products or a control feed balanced in carbohydrate. The onion by-product and the extract caused anaemia as expected in rodents for Allium products. No other toxicity was observed, including genotoxicity. Glutathione reductase (GR) and glutathione peroxidase (GPx1) activities in erythrocytes increased when rats were fed with the onion extract. Hepatic gene expression of Gr, Gpx1, catalase, 5-aminolevulinate synthase and NAD(P)H:quinone oxidoreductase was not altered in any group of the onion fed rats. By contrast, gamma-glutamate cysteine ligase catalytic subunit gene expression was upregulated but only in rats given the onion residue. The onion by-products as well as the soluble and insoluble fractions had prebiotic effects as evidenced by decreased pH, increased butyrate production and altered gut microbiota enzyme activities. In conclusion, the onion by-products have no in vivo genotoxicity, may support in vivo antioxidative defence and alter the functionality of the rat gut microbiota.
Roldán-Marín
Eduvigis
E
Department of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, Søborg, Denmark.
Krath
Britta N
BN
Poulsen
Morten
M
Binderup
Mona-Lise
ML
Nielsen
Tom H
TH
Hansen
Max
M
Barri
Thaer
T
Langkilde
Søren
S
Cano
M Pilar
MP
Sánchez-Moreno
Concepción
C
Dragsted
Lars O
LO
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002432:Cecum / Q000033:anatomy & histology / Q000382:microbiology*; D004249:DNA Damage; D004040:Dietary Carbohydrates / Q000032:analysis; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D005504:Food Analysis / Q000379:methods; D005630:Fructans / Q000032:analysis; D005772:Gastrointestinal Transit / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006418:Heme / Q000096:biosynthesis; D006454:Hemoglobins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008099:Liver / Q000201:enzymology; D008297:Male; D023421:Models, Animal; D009844:Oligosaccharides / Q000032:analysis; D019697:Onions / Q000737:chemistry*; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000009:adverse effects* / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344
D000975:Antioxidants; D004040:Dietary Carbohydrates; D005232:Fatty Acids, Volatile; D005630:Fructans; D006454:Hemoglobins; D009844:Oligosaccharides; D010936:Plant Extracts; C116580:fructooligosaccharide; D006418:Heme