Chronic TNFalpha and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis.
We examined the effects of chronic TNFalpha and dibutyryl-cAMP (Db-cAMP) pre-treatment on the lipolytic machinery of human hMADS adipocytes. TNFalpha decreased adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN) protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In forskolin-stimulated conditions, TNFalpha and Db-cAMP pre-treatment decreased stimulated TG-hydrolase activity and impaired PLIN phosphorylation. Together, this resulted in a severely attenuated response to forskolin-stimulated lipolysis.
Bézaire
Véronic
V
Laboratoire de Recherches sur Obésités, Inserm U858, Toulouse, France.
Mairal
Aline
A
Anesia
Rodica
R
Lefort
Corinne
C
Langin
Dominique
D
D017667:Adipocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002352:Carrier Proteins; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology*; D006801:Humans; D008049:Lipase / Q000032:analysis*; D008066:Lipolysis / Q000187:drug effects*; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation / Q000187:drug effects; D002787:Sterol Esterase / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D000217:Acyltransferases
D002352:Carrier Proteins; D005576:Colforsin; D000242:Cyclic AMP; D008049:Lipase; D000070778:Perilipin-1; D010750:Phosphoproteins; D002787:Sterol Esterase; D014409:Tumor Necrosis Factor-alpha; C494794:PNPLA2 protein, human; D000217:Acyltransferases