Transmission dynamics of Trypanosoma cruzi determined by low-stringency single primer polymerase chain reaction and southern blot analyses in four indigenous communities of the Sierra Nevada de Santa Marta, Colombia.
This study attempted to evaluate the transmission dynamics of Trypanosoma cruzi in four indigenous communities of Sierra Nevada de Santa Marta (SNSM), Colombia. Low-stringency single primer-polymerase chain reaction (LSSP-PCR) of the minicircles and Southern blot analyses were used to characterize samples from patients, vectors, and reservoirs in these communities. The LSSP-PCR profiles revealed a high genetic variability but with similarities among the parasites present in the samples of vectors, patients, and reservoirs of the same and different communities. Cluster and analysis of molecular variance (AMOVA) analyses of data derived from LSSP-PCR and Southern blot suggest a gene flux among populations of T. cruzi circulating in patients, vectors, and reservoirs. The results support the idea that the domestic and wild transmission cycles overlap in the SNSM, with Rhodnius prolixus as the main vector and Triatoma dimidiata playing an important role in the transmission of Chagas disease in this zone, making the vector control strategy by spraying unsuccessful.
Rodríguez
Ingrid B
IB
Grupo Biología y Control de Enfermedades Infecciosas, Universidad de Antioquia, Medellín, Colombia.
Botero
Adriana
A
Mejía-Jaramillo
Ana M
AM
Marquez
Edna J
EJ
Ortiz
Sylvia
S
Solari
Aldo
A
Triana-Chávez
Omar
O
D000818:Animals; D015139:Blotting, Southern; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission*; D003105:Colombia / Q000453:epidemiology; D018105:DNA, Kinetoplast / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D044382:Population Groups; D012239:Rhodnius / Q000469:parasitology; D014224:Triatoma / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000302:isolation & purification
D018105:DNA, Kinetoplast