Microarrays of over 2000 hydrogels--identification of substrates for cellular trapping and thermally triggered release.
In this paper we describe an approach whereby over 2000 individual polymers were synthesized, in situ, on a microscope slide using inkjet printing. Subsequent biological analysis of the entire library allowed the rapid identification of specific polymers with the desired properties. Herein we demonstrate how this array of new materials could be used for the identification of polymers that allow cellular adherence, proliferation and then mild thermal release, for multiple cell lines, including mouse embryonic stem (mES) cells. The optimal, identified hydrogels were successfully scaled-up and demonstrated excellent cell viability after thermal detachment for all cell lines tested. We believe that this approach offers an avenue to the discovery of a specific thermal release polymer for every cell line.
Zhang
Rong
R
School of Chemistry, Kings Building, West Mains Road, University of Edinburgh, Edinburgh EH9 3JJ, UK.
Liberski
Albert
A
Sanchez-Martin
Rosario
R
Bradley
Mark
M
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013696:Temperature
D020100:Hydrogels; D011108:Polymers