[Genetic analysis of the complete env genes of HIV-1 from paid blood donors in Henan province].
Complete HIV-1 env genes were amplified by nested PCR from uncultured peripheral blood mononuclear cells (PBMCs) DNA of 60 HIV-1 positive paid blood donors in Henan province, and the amplified full-length genes were sequenced. Twenty one full-length env genes were obtained, sequence analysis found that 15 of them had intact open reading frame (ORF). Fourteen sequences conformed to subtype B', their average genetic distance with the international reference sequence RL42 was 4.87% +/- 0.31%. One was subtype B, its genetic distance with the international reference sequence HXB2 was 5.43%. The amino acid sequences of these env genes were deduced according to their nucleotide sequences and extensive analysis and comparison of important structural motifs were performed. The results indicated that there was no drastic alteration in the number and position of potential N-linked glycosylation sites among these 15 sequences. And the residues involved in forming the CD4 binding site were highly conserved. Genotype prediction of coreceptor usage based on V3 sequence and net charge suggested that most samples use CCR5 coreceptor. GPGR motif at the tetrapeptide crown in the V3 loop was most common in these samples and it was detected in 40% sequences. The cleavage site of gp120/gp41 was highly conserved, so Gp160 precursor of all isolates would be efficiently cleaved into the Gp120 and Gp41 subunits. The known neutralizing antibody binding sites for 2G12, IgG1b12, 4E10 and 2F5 were also highly conserved, it is expected that most of these isolates will be sensitive to neutralization by these antibodies. Further study to elucidate the correlation of the env genotype to functionally relevant motifs is necessary and that will aid vaccine and novel drug design.
Feng
Xia
X
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China. fengxia621@126.com
Yang
Hai-Ru
HR
Yu
Shuang-Qing
SQ
Zhou
Ling
L
Li
Hong-Xia
HX
Li
Ze-Lin
ZL
Zeng
Yi
Y
D001483:Base Sequence; D001782:Blood Donors / Q000600:supply & distribution*; D015704:CD4 Antigens / Q000378:metabolism; D002681:China; D019411:Clinical Laboratory Techniques; D017124:Conserved Sequence; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D019713:Receptors, CCR5 / Q000737:chemistry / Q000235:genetics*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics*
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D019713:Receptors, CCR5; D054299:env Gene Products, Human Immunodeficiency Virus