title: ErythRED, a hESC line enabling identification of erythroid cells.
abstract: A human embryonic stem cell (hESC) line that enabled globin-expressing cells to be easily recognized would facilitate optimization of erythroid differentiation in vitro and aid in the identification of hESC-derived erythroid cells in transplanted animals. We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human beta-globin locus control region, is restricted to maturing erythroid cells.
authors.affiliation: Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.
authors.forename: Tanya
authors.initials: T
authors.lastname: Hatzistavrou
authors.forename: Suzanne J
authors.initials: SJ
authors.lastname: Micallef
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Ng
authors.forename: Jim
authors.initials: J
authors.lastname: Vadolas
authors.forename: Edouard G
authors.initials: EG
authors.lastname: Stanley
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Elefanty
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D055544:beta-Globins / Q000235:genetics / Q000378:metabolism; D000070584:Tripartite Motif Proteins
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D055544:beta-Globins; C064419:TRIM27 protein, human; D000070584:Tripartite Motif Proteins
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