The dual role of the cystathionine gamma-lyase/hydrogen sulfide pathway in CVB3-induced myocarditis in mice.
The present study found that serum H2S level, H2S production rate, CSE mRNA and CSE protein levels were increased in CVB3-induced myocarditis. dl-proparglygylcine (PAG), an irreversible CSE inhibitor, decreased the infected myocardium titers on postinfection day 4, while NaHS, a H2S donor, alleviated myocardial injury and necrosis, inflammatory cell infiltration and interstitial edema on postinfection day 10. These data reveal that the CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage.
Hua
Wang
W
Department of Pediatric Cardiology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical College, Wenzhou 325027, PR China.
Jiang
Jianbin
J
Rong
Xing
X
Wu
Rongzhou
R
Qiu
Huixian
H
Zhang
Yuanhai
Y
Chen
Qi
Q
D000818:Animals; D003384:Coxsackievirus Infections / Q000150:complications*; D003542:Cystathionine gamma-Lyase / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000097:blood / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000378:metabolism* / Q000473:pathology / Q000821:virology*
D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide