title: Effects of peritoneal fluid from endometriosis patients on interferon-gamma-induced protein-10 (CXCL10) and interleukin-8 (CXCL8) released by neutrophils and CD4+ T cells.
abstract: OBJECTIVE
  Intraperitoneal immuno-inflammatory changes may be associated with the pathogenesis of endometriosis. We evaluated the effects of peritoneal fluid obtained from patients with endometriosis (ePF) on the release of interferon-gamma-induced protein-10 (IP-10/CXCL10) and interleukin-8 (IL-8/CXCL8) by neutrophils, CD4(+) T cells, and monocytes.
  
  
  METHODS
  Neutrophils, CD4(+) T cells, and monocytes were cultured with ePF and the chemokine levels in the supernatants were then measured using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The addition of ePF to cultures of CD4(+) T cells led to a significant increase in the release of IP-10 when compared with control PF without endometriosis (cPF). There was a positive correlation between the levels of IL-8 and IP-10 in ePF (R = 0.89, P = 0.041), but not between the levels of IP-10 and IL-8 released by neutrophils, CD4(+) T cells, and monocytes. The levels of IP-10 in ePF were positively correlated with the release of IP-10 by ePF-treated neutrophils (R = 0.89, P < 0.001), CD4(+) T cells (R = 0.93, P < 0.001), and monocytes (R = 0.70, P = 0.01). Moreover, the addition of ePF significantly enhanced the interferon-gamma-induced release of IP-10 by nuetrophils and CD4(+) T cells.
  
  
  CONCLUSIONS
  These findings suggest that neutrophils and T cells release differential levels of IP-10 and IL-8 in response to stimulation with ePF, and that these cells are a major source of IP-10 in the PF of endometriosis patients.
authors.affiliation: Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, Busan, Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Dong-Hyung
authors.initials: DH
authors.lastname: Lee
authors.forename: Jong-Kil
authors.initials: JK
authors.lastname: Joo
authors.forename: Jun-O
authors.initials: JO
authors.lastname: Jin
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Wang
authors.forename: Young-Seoub
authors.initials: YS
authors.lastname: Hong
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
authors.forename: Kyu-Sup
authors.initials: KS
authors.lastname: Lee
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000276:immunology / Q000378:metabolism*; D018920:Coculture Techniques; D004715:Endometriosis / Q000276:immunology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
chemical_list: C114753:CXCL10 protein, human; C508606:CXCL8 protein, human; D054357:Chemokine CXCL10; D016209:Interleukin-8; D007371:Interferon-gamma