title: [Molecular identification on Waxy genes in wheat using multiple-PCR].
abstract: Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected simultaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality properties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, China. lld5910@yahoo.com
authors.forename: Long-Dou
authors.initials: LD
authors.lastname: Lu
authors.forename: Cai-Ling
authors.initials: CL
authors.lastname: Hou
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Gui-Hong
authors.initials: GH
authors.lastname: Yin
authors.forename: Chuan-Liang
authors.initials: CL
authors.lastname: Deng
authors.forename: Wu-Jun
authors.initials: WJ
authors.lastname: Gao
authors.forename: Xu-Qin
authors.initials: XQ
authors.lastname: Yang
authors.forename: Guang-Xuan
authors.initials: GX
authors.lastname: Tan
mesh_terms: D001947:Breeding; D017931:DNA Primers / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D013214:Starch Synthase / Q000235:genetics* / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D010940:Plant Proteins; C060756:waxy protein, plant; D013214:Starch Synthase