title: A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets.
abstract: DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.
authors.affiliation: Division of Pathway Medicine, University of Edinburgh, Edinburgh, UK EH16 4SB. colin.campbell@ed.ac.uk
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Campbell
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Mountford
authors.forename: Helene C
authors.initials: HC
authors.lastname: Stoquert
authors.forename: Amy H
authors.initials: AH
authors.lastname: Buck
authors.forename: Paul
authors.initials: P
authors.lastname: Dickinson
authors.forename: Elena
authors.initials: E
authors.lastname: Ferapontova
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Terry
authors.forename: John S
authors.initials: JS
authors.lastname: Beattie
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Walton
authors.forename: Jason
authors.initials: J
authors.lastname: Crain
authors.forename: Peter
authors.initials: P
authors.lastname: Ghazal
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Mount
mesh_terms: D015075:2-Aminopurine / Q000737:chemistry*; D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D009711:Nucleotides / Q000235:genetics; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D015342:DNA Probes; D009711:Nucleotides; D015075:2-Aminopurine; D004247:DNA