SNPSTR rs59186128_D7S820 polymorphism distribution in European Caucasoid, Hispanic, and Afro-American populations.
Single nucleotide polymorphisms (SNPs) in the flanking regions of microsatellite loci (SNPSTRs) help to increase the power of discrimination of short tandem repeat (STR) loci. SNPs are positions in the genome that have been well-conserved over the course of evolution, so analysing them can help distinguish between STR alleles in which the number of repetitions matches due to descent from those which match by chance. This provides support for the determination of biological paternity and other kinship analyses in which mutation needs to be ruled out as grounds for exclusion. Locus D7S820 shows a variable position, SNP rs59186128, in the 5' flanking region. This study is set out (1) to determine the frequencies of SNP rs59186128 in populations with various geographical origins and (2) to estimate the possible contribution of rs59186128 to the allele discrimination of locus D7S820. To that end, individuals from European Caucasoid, Hispanic, and Afro-American populations are studied using denaturing high-performance liquid chromatography, which enables locus rs59186128 to be quickly and highly cost-effectively screened. Moreover, a method is established for determining the haplotypes of SNPSTR rs59186128_D7820. The results show that SNP rs59186128 has a T allele frequency of more than 0.15 in one of the Afro-American populations studied, and the haplotype analysis shows that there is no preferential association between the alleles of SNPSTR rs59186128_D7S820, which supports the idea that they could be useful in forensic applications.
Odriozola
A
A
BIOMICS Group, Dept. Z. y Biología Celular A., Facultad de Farmacia, Universidad del País Vasco, 01006 Vitoria-Gasteiz, Alava, Spain.
Aznar
J M
JM
Valverde
L
L
Cardoso
S
S
Bravo
M L
ML
Builes
J J
JJ
Martínez
B
B
Sanchez
D
D
González-Andrade
F
F
Sarasola
E
E
González-Fernández
M C
MC
Martínez Jarreta
B
B
De Pancorbo
Marian M
MM
D002851:Chromatography, High Pressure Liquid; D016172:DNA Fingerprinting; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D044469:Racial Groups / Q000235:genetics*; D020080:Tandem Repeat Sequences
Forensic Sci Int. 2004 Jan 28;139(2-3):159-67
15040910
Forensic Sci Int Genet. 2008 Sep;2(4):292-300
19083839
Mol Biol Evol. 2000 Jun;17(6):929-37
10833199
Mol Ecol Resour. 2008 Jan;8(1):103-6
21585727
Forensic Sci Int. 2001 May 15;118(2-3):116-21
11311822
Int J Legal Med. 2008 Nov;122(6):517-23
18712405
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Oct;22(5):507-9
16215936
Am J Phys Anthropol. 2005 Dec;128(4):855-60
16047324
Forensic Sci Int. 2006 Jul 13;160(2-3):231-5
16188413
Forensic Sci Int. 2006 Jun 2;159(2-3):92-7
16143476
Int J Legal Med. 2008 Sep;122(5):435-40
18491122
Hum Genet. 2007 May;121(3-4):305-17
17333283
Science. 1998 May 15;280(5366):1077-82
9582121
Hum Genomics. 2003 Nov;1(1):7-19
15601529
Mol Ecol. 2002 Dec;11(12):2453-65
12453231
Mutat Res. 2005 Sep 4;577(1-2):275-83
15922366
Nucleic Acids Res. 2007 Jan;35(Database issue):D71-5
17202172
J Forensic Sci. 2002 Jul;47(4):773-85
12136984
N Engl J Med. 2003 Jul 17;349(3):209-10
12867603
Evol Bioinform Online. 2007 Feb 23;1:47-50
19325852
Int J Legal Med. 2008 Sep;122(5):421-7
18581127
Mol Ecol. 2002 Sep;11(9):1591-604
12207711
Curr Opin Hematol. 2004 Sep;11(5):357-61
15666661
Nucleic Acids Res. 1998 Oct 15;26(20):4597-602
9753726
Croat Med J. 2004 Aug;45(4):457-60
15311419
Nat Genet. 2000 Apr;24(4):400-2
10742106