Tracheal suture in rats with hypothyroidism: wound healing study.
OBJECTIVE
To evaluate the influence of hypothyroidism in tracheal wound healing process.


METHODS
A controlled study was designed with 48 male Wistar rats, divided into two groups: study and control groups. In the first one hypothyroidism was surgically induced and 10 weeks after, a tracheal opening followed by suture was performed in both groups, subdivided into 7, 14, and 21 days in accordance with the date of animals death. A laboratorial evaluation was performed to prove the decreased in thyroid function in the study group. Also a macroscopic evaluation through a stablished protocol and a microscopic analysis with Hematoxylin-eosin and Sirius-Red staining methods were done.


RESULTS
The laboratorial evaluation certified suppressed thyroid function in the study group. The macroscopic evaluation showed the presence of suture blockade in the study group in all the evaluated days. Microscopic analysis showed a prolongated inflammatory process and less collagen with delay in organization in the study group comparing to control group. All these data were statistic significant.


CONCLUSIONS
Hypothyroidism had an influence in tracheal wound healing process, promoting delay in the inflammatory and organization processes and diminished tissue collagen quantity.
Zimmermann
Elise
E
Principles of Surgery Post-Graduate Program, Evangelical Medical School, Curitiba, PR, Brazil. ipem@evangelico.org.br
Ribas-Filho
Jurandir Marcondes
JM
Malafaia
Osvaldo
O
Ribas
Carmen Austrália Paredes Marcondes
CA
Nassif
Paulo Afonso Nunes
PA
Stieven Filho
Edmar
E
Przysiezny
Paulo Eduardo
PE
D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D004396:Coloring Agents; D004195:Disease Models, Animal; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D006416:Hematoxylin; D007037:Hypothyroidism / Q000503:physiopathology*; D007249:Inflammation / Q000473:pathology; D008297:Male; D008853:Microscopy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014132:Trachea / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*
D004396:Coloring Agents; D005456:Fluorescent Dyes; D003094:Collagen; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin