title: Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells.
abstract: A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency in comparison to heat shock method and is the first reported chemically-induced transformation. New data about interactions between cholates and biomembranes is revealed that may contribute to better understanding of bacterial transformation.
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000, Ljubljana, Slovenia. matjaz.ravnikar@ffa.uni-lj.si
authors.forename: Matjaz
authors.initials: M
authors.lastname: Ravnikar
authors.forename: Andreja
authors.initials: A
authors.lastname: Irman
authors.forename: Natasa
authors.initials: N
authors.lastname: Radić
authors.forename: Mojca
authors.initials: M
authors.lastname: Lunder
authors.forename: Borut
authors.initials: B
authors.lastname: Strukelj
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects*; D020355:Cholates / Q000378:metabolism*; D012503:Saponins / Q000378:metabolism*; D014169:Transformation, Bacterial
chemical_list: D020355:Cholates; D012503:Saponins