title: Ultrasound-radiated synthesis of PAMAM-Au nanocomposites and its application on glucose biosensor.
abstract: Hybrid nanocomposites of carboxyl-terminated generation 4 (G 4) poly(amidoamine) dendrimers (PAMAM) with gold nanoparticles (AuNPs) encapsulated inside them were synthesized under ultrasound irradiation. The obtained nanocomposites were used to fabricate highly sensitive amperometric glucose biosensor which exhibited a high and reproducible sensitivity of 2.9 mA/mM/cm(2), response time less than 5 s, linear dynamic range from 0.1 to 15.8 microM, correlation coefficient of R(2)=0.9988, and limit of detection (LOD), based on S/N ratio (S/N=3) of 0.05 microM. A value of 2.7 mM for the apparent Michaelis-Menten constant K(M)(app) was obtained. The high sensitivity, wider linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.
authors.affiliation: Department of Chemistry, East China Normal University, 3663 Zhong Shan Road North, Shanghai 200062, China.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Wei
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Ningdan
authors.initials: N
authors.lastname: Zhang
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D050091:Dendrimers; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000528:radiation effects; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry* / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry / Q000528:radiation effects; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry* / Q000528:radiation effects; D013010:Sonication / Q000379:methods*
chemical_list: D050091:Dendrimers; D004800:Enzymes, Immobilized; C104700:PAMAM Starburst; D011073:Polyamines; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose