Polydimethylsiloxane microfluidic chip with integrated microheater and thermal sensor.
A microheater and a thermal sensor were fabricated inside elastomeric polydimethylsiloxane microchannels by injecting silver paint (or other conductive materials) into the channels. With a high-precision control scheme, microheaters can be used for rapid heating, with precise temperature control and uniform thermal distribution. Using such a microheater and feedback system, a polymerase chain reaction experiment was carried out whereas the DNA was successfully amplified in 25 cycles, with 1 min per cycle.
Wu
Jinbo
J
Cao
Wenbin
W
Wen
Weijia
W
Chang
Donald Choy
DC
Sheng
Ping
P
Anal Chem. 2003 Jul 15;75(14):3446-50
14570196
Lab Chip. 2001 Dec;1(2):102-7
15100868
Lab Chip. 2003 Nov;3(4):281-6
15007459
Anal Chem. 2004 Jun 1;76(11):3162-70
15167797
Anal Chem. 2004 Mar 1;76(5):1537-43
14987116
Acc Chem Res. 2002 Jul;35(7):491-9
12118988
Nature. 2006 Jul 27;442(7101):394-402
16871207
Electrophoresis. 2003 Jan;24(1-2):172-8
12652588
Lab Chip. 2004 Dec;4(6):563-9
15570366
Biomicrofluidics. 2008 Jul 21;2(3):34103
19693370
Electrophoresis. 2002 May;23(10):1531-6
12116165
Nature. 2003 Mar 6;422(6927):28-9
12621418
Anal Chem. 2008 Apr 1;80(7):2447-51
18302344
Biotechnol Adv. 2006 May-Jun;24(3):243-84
16326063
Anal Chem. 2005 Jun 15;77(12):3887-93
15952761
Anal Chem. 2003 Sep 1;75(17):4591-8
14632069
Lab Chip. 2004 Aug;4(4):401-7
15269812