title: Development of paratransgenic Artemia as a platform for control of infectious diseases in shrimp mariculture.
abstract: OBJECTIVE
  To study the accumulation and retention of recombinant proteins in Artemia gut for optimizing paratransgenic disease control in shrimp aquaculture.
  
  
  RESULTS
  Transgenic Escherichia coli expressing fluorescent marker proteins and the transgenic cyanobacterium Synechococcus bacillarus expressing a functional murine single chain antibody, DB3, were fed to Artemia franciscana. Stable expression and retention of several marker molecules (e.g. GFP, DS Red and DB3) up to 10 h after of feeding with E. coli were evident within the gut of Artemia. Engineered strains of S. bacillarus expressing DB3 accumulated within the gut of Artemia with detectable antibody activity for 8-10 h of feeding via ELISA, coincident with the time period of the highest density of transgenic S. bacillarus in the Artemia gut.
  
  
  CONCLUSIONS
  Artemia fed transgenic bacteria or algae accumulated recombinant proteins for up to 10 h that retained biological activity. Co-delivery of multiple recombinant proteins simultaneously in the gut of Artemia was also demonstrated.
  
  
  CONCLUSIONS
  Expression of molecules that target infectious agents of mariculture in shrimp via commonly deployed feed organisms such as Artemia could potentially offer powerful new tools in the ongoing global effort to increase food supply.
authors.affiliation:  Department of Internal Medicine, School of Medicine, University of New Mexico, Albuquerque, NM, USA.
authors.forename: B
authors.initials: B
authors.lastname: Subhadra
authors.affiliation:  Department of Internal Medicine, School of Medicine, University of New Mexico, Albuquerque, NM, USA.
authors.forename: I
authors.initials: I
authors.lastname: Hurwitz
authors.affiliation:  Department of Internal Medicine, School of Medicine, University of New Mexico, Albuquerque, NM, USA.
authors.forename: A
authors.initials: A
authors.lastname: Fieck
authors.affiliation:  Emeritus Scientist, Bedford Institute of Oceanography, Dartmouth, NS, Canada.
authors.forename: D V S
authors.initials: DVS
authors.lastname: Rao
authors.affiliation:  Shree Vasudha Laboratories, Visakhapatnam, India.
authors.forename: G
authors.initials: G
authors.lastname: Subba Rao
authors.affiliation:  Department of Internal Medicine, School of Medicine, University of New Mexico, Albuquerque, NM, USA.
authors.forename: R
authors.initials: R
authors.lastname: Durvasula
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D001156:Artemia / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics*; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D030781:Organisms, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012758:Shellfish / Q000382:microbiology; D057127:Single-Chain Antibodies / Q000235:genetics / Q000378:metabolism*; D046940:Synechococcus / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D049452:Green Fluorescent Proteins
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