Serogroup, virulence, and genetic traits of Vibrio parahaemolyticus in the estuarine ecosystem of Bangladesh.
Forty-two strains of Vibrio parahaemolyticus were isolated from Bay of Bengal estuaries and, with two clinical strains, analyzed for virulence, phenotypic, and molecular traits. Serological analysis indicated O8, O3, O1, and K21 to be the major O and K serogroups, respectively, and O8:K21, O1:KUT, and O3:KUT to be predominant. The K antigen(s) was untypeable, and pandemic serogroup O3:K6 was not detected. The presence of genes toxR and tlh were confirmed by PCR in all but two strains, which also lacked toxR. A total of 18 (41%) strains possessed the virulence gene encoding thermostable direct hemolysin (TDH), and one had the TDH-related hemolysin (trh) gene, but not tdh. Ten (23%) strains exhibited Kanagawa phenomenon that surrogates virulence, of which six, including the two clinical strains, possessed tdh. Of the 18 tdh-positive strains, 17 (94%), including the two clinical strains, had the seromarker O8:K21, one was O9:KUT, and the single trh-positive strain was O1:KUT. None had the group-specific or ORF8 pandemic marker gene. DNA fingerprinting employing pulsed-field gel electrophoresis (PFGE) of SfiI-digested DNA and cluster analysis showed divergence among the strains. Dendrograms constructed using PFGE (SfiI) images from a soft database, including those of pandemic and nonpandemic strains of diverse geographic origin, however, showed that local strains formed a cluster, i.e., "clonal cluster," as did pandemic strains of diverse origin. The demonstrated prevalence of tdh-positive and diarrheagenic serogroup O8:K21 strains in coastal villages of Bangladesh indicates a significant human health risk for inhabitants.
Alam
Munirul
M
International Center for Diarrhoeal Disease Research, Bangladesh, Mohakhali, Dhaka 1212, Bangladesh.
Chowdhury
Wasimul B
WB
Bhuiyan
N A
NA
Islam
Atiqul
A
Hasan
Nur A
NA
Nair
G Balakrish
GB
Watanabe
H
H
Siddique
A K
AK
Huq
Anwar
A
Sack
R Bradley
RB
Akhter
M Z
MZ
Grim
Christopher J
CJ
Kam
K-M
KM
Luey
C K Y
CK
Endtz
Hubert P
HP
Cravioto
Alejandro
A
Colwell
Rita R
RR
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001459:Bangladesh; D044822:Biodiversity; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D006801:Humans; D017720:Molecular Epidemiology; D012703:Serotyping; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D037521:Virulence Factors
Epidemiol Infect. 2000 Aug;125(1):17-25
11057955
J Infect Dis. 1995 Nov;172(5):1405-8
7594689
J Clin Microbiol. 2008 Aug;46(8):2766-73
18579720
Emerg Infect Dis. 2005 Jan;11(1):129-31
15705337
J Clin Microbiol. 1989 Dec;27(12):2820-2
2592543
Microbiol Res. 2006;161(1):25-37
16338587
FEMS Microbiol Lett. 2002 Feb 19;208(1):83-7
11934498
JAMA. 2000 Sep 27;284(12):1541-5
11000648
Infect Immun. 1992 Sep;60(9):3539-45
1500161
Appl Environ Microbiol. 2008 Jan;74(1):265-74
17981951
Appl Environ Microbiol. 2006 Jun;72(6):4096-104
16751520
J Med Microbiol. 2007 May;56(Pt 5):608-613
17446282
J Clin Microbiol. 2004 Oct;42(10):4672-8
15472326
J Clin Microbiol. 1999 Apr;37(4):1173-7
10074546
J Microbiol Methods. 1999 Jun;36(3):215-25
10379807
Infect Immun. 1995 Jun;63(6):2093-9
7768586
Infect Immun. 1975 Jul;12(1):81-7
237836
Appl Environ Microbiol. 2000 Sep;66(9):3981-6
10966418
Int J Food Microbiol. 1999 Nov 15;52(3):181-8
10733249
Microb Pathog. 1986 Oct;1(5):425-32
3508495
Epidemiol Infect. 2003 Jun;130(3):395-406
12825723
Rev Chilena Infectol. 2005 Jun;22(2):125-30
15891792
J Clin Microbiol. 2005 Jun;43(6):2559-62
15956363
J Clin Microbiol. 2000 Jun;38(6):2156-61
10834969
J Clin Microbiol. 2002 Jan;40(1):284-6
11773134
Nucleic Acids Res. 1991 Dec 25;19(24):6823-31
1762913
J Clin Microbiol. 2004 Mar;42(3):1280-2
15004094
J Clin Microbiol. 2001 Dec;39(12):4233-40
11724826
Appl Environ Microbiol. 2000 Nov;66(11):4649-54
11055906
Appl Environ Microbiol. 2004 Nov;70(11):6401-6
15528498
J Clin Microbiol. 2000 Feb;38(2):578-85
10655349
J Clin Microbiol. 1997 Dec;35(12):3150-5
9399511
J Clin Microbiol. 1999 Jul;37(7):2354-7
10364615
Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15442-7
19720995
J Bacteriol. 1973 Jan;113(1):24-32
4567138
Appl Environ Microbiol. 2001 Dec;67(12):5819-23
11722939
J Clin Microbiol. 1995 Sep;33(9):2233-9
7494007