title: Detection of Plasmodium knowlesi by real-time polymerase chain reaction.
abstract: We previously developed a real-time polymerase chain reaction (PCR) assay for detection of the four Plasmodium species that infect humans. Recent studies have shown that natural transmission of the simian parasite Plasmodium knowlesi to humans occurs frequently in Southeast Asia. We have expanded our PCR assay to include detection of P. knowlesi.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: N Esther
authors.initials: NE
authors.lastname: Babady
authors.forename: Lynne M
authors.initials: LM
authors.lastname: Sloan
authors.forename: Jon E
authors.initials: JE
authors.lastname: Rosenblatt
authors.forename: Bobbi S
authors.initials: BS
authors.lastname: Pritt
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000145:classification / Q000302:isolation & purification; D004854:Herpesvirus 4, Human; D054884:Host-Pathogen Interactions; D016790:Plasmodium knowlesi / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity; D014122:Toxoplasma; D054851:Tropheryma
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S