title: Evidence for developmentally regulated local translation of odorant receptor mRNAs in the axons of olfactory sensory neurons.
abstract: Odorant receptor mRNAs are transported within axons of olfactory sensory neurons that project into the olfactory bulb. Odorant receptor proteins have been identified along the distal part of these axons, which raises the possibility of their local synthesis in axons. We took advantage of the anatomical separation between the olfactory mucosa (which contains the sensory neuron cell bodies) and the bulb (which contains sensory axons but no sensory neuron cell bodies) to address this issue using a quantitative biochemical approach. Combining a method that separates polysome-associated mRNAs from untranslated mRNAs with a reverse transcription-quantitative PCR approach, we demonstrate that significant amounts of odorant receptor mRNAs are associated with polysomes in the sensory axons of the adult mouse bulb. We thus provide the first evidence for local synthesis of odorant receptor proteins in these axons. Interestingly, the rate of odorant receptor mRNA translation in axons is significantly greater during periods when the proportion of immature axons is higher (i.e., at postnatal day 4 or on regeneration after chemical lesion in adults). In contrast, the olfactory marker protein mRNA, which is restricted to mature axons, is translated at a low and constant level. Overall, we demonstrate that translation levels of odorant receptor mRNAs in axons are developmentally regulated, and positively correlated to the stage of axonal growth into the bulb. Given the established function of odorant receptors in the axonal wiring of sensory projections, we propose that this regulated axonal translation may play a role in the development and maintenance of the glomerular array.
authors.affiliation: Avenir/Inserm and Fondation pour la Recherche Médicale Team Development and Plasticity of Neural Networks, Université Pierre et Marie Curie (UPMC), Unité Mixte de Recherche (UMR) 7102, and Centre National de la Recherche Scientifique, UMR 7102, Paris, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Dubacq
authors.forename: Sophie
authors.initials: S
authors.lastname: Jamet
authors.forename: Alain
authors.initials: A
authors.lastname: Trembleau
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008297:Male; D051379:Mice; D018034:Olfactory Receptor Neurons / Q000254:growth & development* / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D018035:Receptors, Odorant / Q000096:biosynthesis* / Q000235:genetics*; D011984:Sensory Receptor Cells / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D018035:Receptors, Odorant
references.citation: Cell. 2002 Jul 26;110(2):223-35
references.pmid: 12150930
references.citation: J Neurosci. 1995 Jul;15(7 Pt 1):4827-37
references.pmid: 7623114
references.citation: Eur J Neurosci. 1991;3(3):209-215
references.pmid: 12106197
references.citation: Annu Rev Cell Dev Biol. 2006;22:713-37
references.pmid: 17029582
references.citation: Science. 2004 Jun 4;304(5676):1468
references.pmid: 15178793
references.citation: Cell Mol Neurobiol. 2007 Sep;27(6):701-16
references.pmid: 17619140
references.citation: Curr Opin Neurobiol. 2004 Feb;14(1):31-6
references.pmid: 15018935
references.citation: Science. 2006 Oct 27;314(5799):657-61
references.pmid: 16990513
references.citation: Cell. 2005 Nov 18;123(4):669-82
references.pmid: 16290037
references.citation: Neuron. 2006 Sep 21;51(6):685-90
references.pmid: 16982415
references.citation: Nat Neurosci. 2006 Oct;9(10):1247-56
references.pmid: 16980963
references.citation: J Neurosci. 2009 Jan 21;29(3):638-52
references.pmid: 19158291
references.citation: Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19919-24
references.pmid: 17167054
references.citation: Science. 1999 Oct 22;286(5440):711-5
references.pmid: 10531048
references.citation: Nat Cell Biol. 2008 Feb;10(2):149-59
references.pmid: 18193038
references.citation: Neuron. 2001 Dec 20;32(6):1013-26
references.pmid: 11754834
references.citation: Traffic. 2006 Dec;7(12):1581-9
references.pmid: 17054760
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3537-42
references.pmid: 19218439
references.citation: Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14168-73
references.pmid: 15377787
references.citation: EMBO J. 2007 Aug 22;26(16):3729-36
references.pmid: 17660744
references.citation: J Neurocytol. 2005 Mar;34(1-2):49-64
references.pmid: 16374709
references.citation: Nature. 2005 Nov 3;438(7064):94-8
references.pmid: 16267555
references.citation: Cell. 1991 Apr 5;65(1):175-87
references.pmid: 1840504
references.citation: J Comp Neurol. 1998 Jan 12;390(2):256-67
references.pmid: 9453669
references.citation: J Neurosci. 1990 Jun;10(6):1952-66
references.pmid: 2355260
references.citation: Cell. 1994 Dec 30;79(7):1245-55
references.pmid: 7528109
references.citation: Cell. 2006 Dec 1;127(5):1057-69
references.pmid: 17129788
references.citation: Cell. 1994 Dec 16;79(6):981-91
references.pmid: 8001145
references.citation: Nature. 2005 Aug 18;436(7053):1020-1024
references.pmid: 16107849
references.citation: Cell. 2004 Jun 11;117(6):833-46
references.pmid: 15186782
references.citation: J Neurosci. 2000 May 15;20(10):RC76
references.pmid: 10783400
references.citation: Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10815-20
references.pmid: 15247416
references.citation: Curr Opin Neurobiol. 2006 Feb;16(1):111-8
references.pmid: 16418002
references.citation: J Neurosci. 1994 Jan;14(1):39-53
references.pmid: 8283246
references.citation: J Neurosci. 2004 Sep 1;24(35):7754-61
references.pmid: 15342743
references.citation: J Neurosci. 1999 Oct 1;19(19):8409-18
references.pmid: 10493742
references.citation: Physiol Rev. 2008 Apr;88(2):515-55
references.pmid: 18391172
references.citation: Mol Cell Neurosci. 2009 Feb;40(2):128-42
references.pmid: 19022387
references.citation: J Comp Neurol. 1999 Oct 18;413(2):289-304
references.pmid: 10524340
references.citation: Chem Senses. 2005 Jan;30 Suppl 1:i131-2
references.pmid: 15738075
references.citation: J Neurosci. 2002 Apr 1;22(7):2469-77
references.pmid: 11923411
references.citation: Nat Neurosci. 2006 Oct;9(10):1265-73
references.pmid: 16980965
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1039-44
references.pmid: 17215378