[Expression of chosen chemokine receptors on the Th lymphocytes in children with hypertrophied adenoids with otitis media with effusion].
BACKGROUND
Chemokine receptors play a crucial role in the recruitment of leucocytes into inflamed tissue from secondary lymphoid organs.


OBJECTIVE
was evaluation of the percentage Th (CD4+) lymphocytes with expression of chemokine receptors: CCR3, CCR4, and CCR5 in hypertrophied adenois tissue in children with otitis media with effusion.


METHODS
36 children with otitis media with effusion and 25 children with hypertrophied adenoids were tested. Expression of chemokine receptors CCR3, CCR4 and CCR5 on T CD4 lymphocytes of hypertrophied adenoid's tissue was estimated by flow cytometry method.


RESULTS
Average percentage of T CD4+ lymphocytes with expression CCR4 in hypertrophied adenoid in children group with otitis media with effusion (OME = 64.11%) was significantly higher than in comparative group with hypertrophied adenoid (HA = 75.05%, p < 0.04). We showed statistically lower percentage of CD3+CD4+ cells with expression CCR3 in examinated group (OME = 47.19%) than HA group (62.66%; p < 0.003).


CONCLUSIONS
Results suggests that intensify of humoral immunological answer in hypertrophied adenoid depends on Th2 lymphocytes with expression CCR3 and CCR4 chemokine receptors in course of inflammatory state in middle ear.
Ratomski
Karol
K
Zakład Laboratoryjnej Diagnostyki Pediatrycznej, Uniwersytet Medyczny w Białymstoku. karol.cam@interia.pl
Wysocka
Jolanta
J
Skotnicka
Bozena
B
Kasprzycka
Edwina
E
Zelazowska-Rutkowska
Beata
B
Zak
Janusz
J
Hassmann-Poznańska
Elzbieta
E
D000234:Adenoids / Q000276:immunology* / Q000601:surgery; D000293:Adolescent; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006984:Hypertrophy / Q000276:immunology; D007223:Infant; D008297:Male; D010034:Otitis Media with Effusion / Q000276:immunology* / Q000601:surgery; D054388:Receptors, CCR / Q000378:metabolism*; D054397:Receptors, CCR3 / Q000378:metabolism; D054398:Receptors, CCR4 / Q000378:metabolism; D019713:Receptors, CCR5 / Q000378:metabolism; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
D015415:Biomarkers; D054388:Receptors, CCR; D054397:Receptors, CCR3; D054398:Receptors, CCR4; D019713:Receptors, CCR5