title: Radiation stability of proteinase K crystals grown by LB nanotemplate method.
abstract: A detailed analysis of structural and intensity changes induced by X-ray radiation is presented for two types of proteinase K crystals: crystal grown by classical hanging drop method and those grown by Langmuir-Blodgett (LB) nanotemplate. The comparison of various parameters (e.g. intensity per sigma ratio, unit-cell volume, number of unique reflections, B-factors) and electron density maps as a function of radiation dose, demonstrates that crystals, grown by the LB nanotemplate method, appear to be more resistant against radiation damage than crystals grown by the classical hanging drop method.
authors.affiliation: Nanoworld Institute, CIRSDNNOB University of Genova, Genoa, Italy.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Pechkova
authors.forename: Shailesh
authors.initials: S
authors.lastname: Tripathi
authors.forename: Raimond B G
authors.initials: RB
authors.lastname: Ravelli
authors.forename: Sean
authors.initials: S
authors.lastname: McSweeney
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicolini
mesh_terms: D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D019286:Endopeptidase K / Q000737:chemistry*; D004795:Enzyme Stability / Q000528:radiation effects; D018625:Microscopy, Atomic Force; D014965:X-Rays / Q000009:adverse effects
chemical_list: D019286:Endopeptidase K