Down-regulation of Pkd2 by siRNAs suppresses cell-cell adhesion in the mouse melanoma cells.
The Pkd2 gene encodes an integral protein (~130 kDa), named polycystin-2 (PC-2). PC-2 is mainly involved in autosomal dominant polycystic kidney disease. Recently, polycystin-1/polycystin-2 complex has been shown to act as an adhesion complex mediating or regulating cell-cell or cell-matrix adhesion, suggesting that PC-2 may play a role in cell-cell/cell-matrix interactions. Here, we knocked down the expression of Pkd2 gene with small interfering RNAs (siRNAs) in the mouse melanoma cells (B16 cells), indicating that the cells transfected with the targeted siRNAs significantly suppressed cell-cell adhesion, but not cell-matrix adhesion, compared to the cells transfected with non-targeted control (NC) siRNA. This study provides the first directly functional evidence that PC-2 mediates cell-cell adhesion. Furthermore, we demonstrated that PC-2 modulated cell-cell adhesion may be, at least partially, associated with E-cadherin. Collectively, these findings for the first time showed that PC-2 may mediate cell-cell adhesion, at least partially, through E-cadherin.
Bian
Guo-Hui
GH
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Keyuan the Fourth Road, The District of Hi&Tech, 610041 Chengdu, People's Republic of China.
Cao
Ge
G
Lv
Xiao-Yan
XY
Li
Qing-Wei
QW
Sun
Huan
H
Xiao
Yan
Y
Ai
Jian-Zhong
JZ
Yang
Qui-Tan
QT
Duan
Jing-Jing
JJ
Wang
Yi-Dong
YD
Zhang
Zheng
Z
Tan
Rui-Zhi
RZ
Liu
Yu-Hang
YH
Yang
Yang
Y
Wei
Yu-Quan
YQ
Zhou
Qin
Q
D000818:Animals; D001681:Biological Assay; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D045744:Cell Line, Tumor; D021982:Cell-Matrix Junctions / Q000378:metabolism; D024042:Collagen Type I / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008545:Melanoma / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D050396:TRPP Cation Channels / Q000235:genetics* / Q000378:metabolism
D015820:Cadherins; D024042:Collagen Type I; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050396:TRPP Cation Channels
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