title: Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells.
abstract: Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microg/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 020-013, Egypt. mohamedsoliman8896@yahoo.com
authors.forename: Mohamed Mohamed
authors.initials: MM
authors.lastname: Soliman
authors.forename: Yakut Abdel-Fattah
authors.initials: YA
authors.lastname: El-Senosi
authors.forename: Maysara Mahmoud
authors.initials: MM
authors.lastname: Salem
authors.forename: Omniya Mahmoud
authors.initials: OM
authors.lastname: Abdel Hamid
authors.forename: Kimura
authors.initials: K
authors.lastname: Kazuhiro
mesh_terms: D016475:3T3 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D003176:Complement C3; D005786:Gene Expression Regulation / Q000187:drug effects*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D011480:Protease Inhibitors / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C545610:C3 protein, mouse; D003176:Complement C3; D007004:Hypoglycemic Agents; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D011480:Protease Inhibitors
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