title: Organ-specific distributions of lysophosphatidylcholine and triacylglycerol in mouse embryo.
abstract: Imaging mass spectrometry (IMS) has been developed as a method for determining and visualizing the distribution of proteins and lipids across sections of dissected tissue. Although lipids play an important role in mammal development, their detailed distributions have not been analyzed by conventional methods. In this study, we tried to determine and visualize lysophosphatidylcholine (LysoPtdCho) and triacylglycerol (TAG) in a mouse embryo by matrix-assisted laser desorption/ionization (MALDI) hybrid quadrupole time-of-flight (TOF) mass spectrometer. Many peaks were detected from a raster scan of the whole embryonic sections. The peaks at m/z 496.33, 524.36, 879.72, 881.74, and 921.74 were identified by MS/MS analyses as [LysoPtdCho (16:0) + H](+), [LysoPtdCho (18:0) + H](+), [TAG (16:0/18:2/18:1) + Na](+), [TAG (16:0/18:1/18:1) + Na](+), and [TAG (16:0/20:3/18:1) + K](+), respectively. The ion images constructed from the peaks revealed that LysoPtdCho were distributed throughout the body and TAGs were distributed around the brown adipose tissue and in the liver at embryo day 17.5. Thus, IMS system based on MALDI hybrid quadrupole TOF MS revealed the distribution of LysoPtdCho and, more importantly, the organ-specific distribution of TAGs in the embryonic stages of mammals for the first time. We can conclude that this technique enables us to analyze the roles of various lipids during embryogenesis and gives insight for lipid research.
authors.affiliation: Department of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Zaima
authors.forename: Yoshishige
authors.initials: Y
authors.lastname: Kimura
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000033:anatomy & histology* / Q000737:chemistry*; D005260:Female; D008244:Lysophosphatidylcholines / Q000032:analysis*; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000032:analysis*
chemical_list: D008244:Lysophosphatidylcholines; D014280:Triglycerides
references.citation: Anal Chem. 1991 Dec 15;63(24):1193A-1203A
references.pmid: 1789447
references.citation: Transgenic Res. 2008 Oct;17(5):955-63
references.pmid: 18523856
references.citation: Anal Chem. 2006 Dec 15;78(24):8227-35
references.pmid: 17165811
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18120-5
references.pmid: 18697929
references.citation: J Am Soc Mass Spectrom. 1999 Jul;10(7):587-99
references.pmid: 10384723
references.citation: Anal Chem. 2008 Feb 1;80(3):878-85
references.pmid: 18166020
references.citation: J Histochem Cytochem. 2007 Sep;55(9):925-30
references.pmid: 17478445
references.citation: Curr Drug Targets. 2008 Nov;9(11):922-30
references.pmid: 18991604
references.citation: Biochem Biophys Res Commun. 2007 Oct 19;362(2):252-5
references.pmid: 17707337
references.citation: N Engl J Med. 2008 Nov 27;359(22):2396-8
references.pmid: 19038890
references.citation: Int J Mass Spectrom. 2007 Feb 1;260(2-3):212-221
references.pmid: 17541451
references.citation: Anal Chem. 2008 Jun 15;80(12):4761-6
references.pmid: 18476721
references.citation: Nature. 2002 May 2;417(6884):83-7
references.pmid: 11986669
references.citation: Cell. 2007 Sep 7;130(5):943-57
references.pmid: 17803915
references.citation: Am J Clin Nutr. 2008 Mar;87(3):548-57
references.pmid: 18326591
references.citation: J Lipid Res. 2005 Jul;46(7):1388-95
references.pmid: 15834124
references.citation: PLoS One. 2008 Sep 18;3(9):e3232
references.pmid: 18800170
references.citation: J Am Soc Mass Spectrom. 2008 Jun;19(6):823-32
references.pmid: 18403214
references.citation: Proteomics. 2008 Sep;8(18):3692-701
references.pmid: 18780397
references.citation: Anal Chem. 1988 Oct 15;60(20):2299-301
references.pmid: 3239801
references.citation: Proc Natl Acad Sci U S A. 2003 May 27;100(11):6358-63
references.pmid: 12738878
references.citation: Rapid Commun Mass Spectrom. 2003;17(1):87-96
references.pmid: 12478559
references.citation: Anal Chem. 2006 Sep 15;78(18):6448-56
references.pmid: 16970320
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3213-8
references.pmid: 17360631
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jul 1;870(1):74-83
references.pmid: 18571485
references.citation: J Proteome Res. 2008 Aug;7(8):3543-55
references.pmid: 18613713
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug;855(1):98-103
references.pmid: 17369111
references.citation: Brain Res. 2008 Oct 27;1237:136-45
references.pmid: 18710653
references.citation: Mol Cell Biochem. 2001 Sep;225(1-):109-19
references.pmid: 11716352
references.citation: J Am Soc Mass Spectrom. 2003 Apr;14(4):352-63
references.pmid: 12686482
references.citation: Neurosci Res. 2007 Mar;57(3):411-23
references.pmid: 17207874
references.citation: Prostaglandins Leukot Essent Fatty Acids. 2008 Jan;78(1):73-9
references.pmid: 18093817
references.citation: Anal Chem. 2007 Sep 1;79(17):6862-7
references.pmid: 17658766
references.citation: J Lipid Res. 2009 Apr;50 Suppl:S317-22
references.pmid: 19050313
references.citation: Rapid Commun Mass Spectrom. 2008 Nov;22(21):3415-26
references.pmid: 18837478
references.citation: Rapid Commun Mass Spectrom. 1993 Oct;7(10):902-10
references.pmid: 8219321
references.citation: Arzneimittelforschung. 2008;58(9):469-74
references.pmid: 18972878
references.citation: Endocrinology. 2008 Jul;149(7):3274-8
references.pmid: 18403475
references.citation: Lipids. 1996 Mar;31(3):313-21
references.pmid: 8900461
references.citation: Prog Lipid Res. 1997 Sep;36(2-3):85-101
references.pmid: 9624423
references.citation: Curr Opin Biotechnol. 2006 Aug;17(4):431-6
references.pmid: 16781865
references.citation: Nature. 2007 Oct 25;449(7165):1033-6
references.pmid: 17960240
references.citation: Am J Clin Nutr. 1998 Mar;67(3):377-85
references.pmid: 9497179
references.citation: Lipids. 1988 Jan;23(1):32-5
references.pmid: 3352470
references.citation: Prep Biochem. 1980;10(4):463-71
references.pmid: 7413608
references.citation: J Am Soc Mass Spectrom. 2008 Aug;19(8):1069-77
references.pmid: 18472274
references.citation: Cell. 2004 Dec 17;119(6):889-900
references.pmid: 15607983
references.citation: J Mass Spectrom. 2007 Feb;42(2):254-62
references.pmid: 17230433
references.citation: AAPS J. 2006 Jun 02;8(2):E391-5
references.pmid: 16796390
references.citation: Science. 2000 Jun 9;288(5472):1796-802
references.pmid: 10846156
references.citation: J Am Soc Mass Spectrom. 2005 Sep;16(9):1498-1509
references.pmid: 16019221
references.citation: Lipids. 1999 Aug;34(8):865-71
references.pmid: 10529098
references.citation: J Lipid Res. 1995 Jan;36(1):125-36
references.pmid: 7706937