title: Limitations in SELDI-TOF MS whole serum proteomic profiling with IMAC surface to specifically detect colorectal cancer.
abstract: BACKGROUND
  Surface enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) analysis on serum samples was reported to be able to detect colorectal cancer (CRC) from normal or control patients. We carried out a validation study of a SELDI-TOF MS approach with IMAC surface sample processing to identify CRC.
  
  
  METHODS
  A retrospective cohort of 338 serum samples including 154 CRCs, 67 control cancers and 117 non-cancerous conditions was profiled using SELDI-TOF-MS.
  
  
  RESULTS
  No CRC "specific" classifier was found. However, a classifier consisting of two protein peaks separates cancer from non-cancerous conditions with high accuracy.
  
  
  CONCLUSIONS
  In this study, the SELDI-TOF-MS-based protein expression profiling approach did not perform to identify CRC. However, this technique is promising in distinguishing patients with cancer from a non-cancerous population; it may be useful for monitoring recurrence of CRC after treatment.
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Colorectal Surgery, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, PR China. wangki2004@126.com
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.forename: Zhen-fu
authors.initials: ZF
authors.lastname: Li
authors.forename: Jian-zheng
authors.initials: JZ
authors.lastname: Jie
authors.forename: Wen-yue
authors.initials: WY
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Zhong-tao
authors.initials: ZT
authors.lastname: Zhang
authors.forename: Zhi-xia
authors.initials: ZX
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Jin
authors.initials: J
authors.lastname: Gu
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012189:Retrospective Studies; D044967:Serum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor
references.citation: Clin Chem. 2002 Aug;48(8):1296-304
references.pmid: 12142387
references.citation: Ann Intern Med. 2008 Oct 7;149(7):441-50, W81
references.pmid: 18838724
references.citation: Clin Chem. 2007 Apr;53(4):645-56
references.pmid: 17303688
references.citation: Zhonghua Liu Xing Bing Xue Za Zhi. 2005 May;26(5):366-9
references.pmid: 16053766
references.citation: Cancer Res. 2002 Jul 1;62(13):3609-14
references.pmid: 12097261
references.citation: Eur J Cancer. 2003 Apr;39(6):718-27
references.pmid: 12651195
references.citation: J Natl Cancer Inst. 2001 Jul 18;93(14):1054-61
references.pmid: 11459866
references.citation: Cancer Inform. 2005;1:86-97
references.pmid: 19305634
references.citation: Lancet. 1996 Nov 30;348(9040):1467-71
references.pmid: 8942774
references.citation: Clin Chem. 2008 Jan;54(1):44-52
references.pmid: 17981926
references.citation: Cancer Res. 2005 Nov 15;65(22):10613-22
references.pmid: 16288055
references.citation: J Natl Cancer Inst. 2004 Mar 3;96(5):353-6
references.pmid: 14996856
references.citation: Clin Chem. 2008 Jan;54(1):53-60
references.pmid: 18024530
references.citation: Ann Thorac Surg. 2007 Aug;84(2):384-92; discussion 392
references.pmid: 17643604
references.citation: Clin Chem. 2005 Jan;51(1):102-12
references.pmid: 15613711
references.citation: Br J Cancer. 2006 Jun 19;94(12):1898-905
references.pmid: 16755300
references.citation: Am J Gastroenterol. 2008 Nov;103(11):2862-70
references.pmid: 18759824
references.citation: J Urol. 2003 Aug;170(2 Pt 1):442-6
references.pmid: 12853795
references.citation: BMC Cancer. 2005 Jan 19;5:8
references.pmid: 15656915
references.citation: CA Cancer J Clin. 2000 Jan-Feb;50(1):34-49
references.pmid: 10735014
references.citation: Br J Cancer. 2006 Jan 30;94(2):287-92
references.pmid: 16404431
references.citation: J Natl Cancer Inst. 2005 Feb 16;97(4):315-9
references.pmid: 15713968
references.citation: Clin Chem. 2003 May;49(5):752-60
references.pmid: 12709366
references.citation: Lancet. 2002 Feb 16;359(9306):572-7
references.pmid: 11867112
references.citation: J Urol. 2005 Oct;174(4 Pt 1):1213-7
references.pmid: 16145372
references.citation: Clin Chem. 2001 Apr;47(4):624-30
references.pmid: 11274010
references.citation: Nat Rev Cancer. 2005 Feb;5(2):142-9
references.pmid: 15685197
references.citation: Am J Pathol. 2001 Apr;158(4):1491-502
references.pmid: 11290567
references.citation: Cancer Invest. 2006 Dec;24(8):747-53
references.pmid: 17162557
references.citation: Clin Cancer Res. 2004 Dec 15;10(24):8380-5
references.pmid: 15623616