Targeted cell detection based on microchannel gating.
Currently, microbiological techniques such as culture enrichment and various plating techniques are used for detection of pathogens. These expensive and time consuming methods can take several days. Described below is the design, fabrication, and testing of a rapid and inexpensive sensor, involving the use of microelectrodes in a microchannel, which can be used to detect single bacterial cells electrically (label-free format) in real time. As a proof of principle, we have successfully demonstrated real-time detection of target yeast cells by measuring instantaneous changes in ionic impedance. We have also demonstrated the selectivity of our sensors in responding to target cells while remaining irresponsive to nontarget cells. Using this technique, it can be possible to multiplex an array of these sensors onto a chip and probe a complex mixture for various types of bacterial cells.
Javanmard
Mehdi
M
Electrical Engineering Department, Stanford University, Stanford, California 94305, USA and Stanford Genome Technology Center, Palo Alto, California 94304, USA.
Talasaz
Amirali H
AH
Nemat-Gorgani
Mohsen
M
Pease
Fabian
F
Ronaghi
Mostafa
M
Davis
Ronald W
RW
Biosens Bioelectron. 2004 Jun 15;19(11):1497-504
15093222
Biosens Bioelectron. 2004 May 15;19(10):1139-47
15046744
Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
10511517
Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):820-4
12552089
Biosens Bioelectron. 2007 Apr 15;22(9-10):2329-36
17320373
Lab Chip. 2005 Oct;5(10):1155-60
16175273
Lab Chip. 2007 May;7(5):603-10
17476379
Lab Chip. 2006 May;6(5):682-92
16652185
Lab Chip. 2001 Sep;1(1):76-82
15100895
Biosens Bioelectron. 2003 Dec 30;19(5):495-502
14623474
J Clin Microbiol. 1986 May;23(5):869-72
3519658
Biomed Eng Online. 2005 Mar 29;4:22
15796776