Gravity separation of pericardial fat in cardiotomy suction blood: an in vitro model.
Fat emboli generated during cardiac surgery have been shown to cause neurologic complications in patients postoperatively. Cardiotomy suction has been known to be a large generator of emboli. This study will examine the efficacy of a separation technique in which the cardiotomy suction blood is stored in a cardiotomy reservoir for various time intervals to allow spontaneous separation of fat from blood by density. Soybean oil was added to heparinized porcine blood to simulate the blood of a patient with hypertriglyceridemia (> 150 mg/dL). Roller pump suction was used to transfer the room temperature blood into the cardiotomy reservoir. Blood was removed from the reservoir in 200-mL aliquots at 0, 15, 30 45, and 60 minutes. Samples were taken at each interval and centrifuged to facilitate further separation of liquid fat. Fat content in each sample was determined by a point-of-care triglyceride analyzer. Three trials were conducted for a total of 30 samples. The 0-minute group was considered a baseline and was compared to the other four times. Fat concentration was reduced significantly in the 45- and 60-minute groups compared to the 0-, 15-, and 30-minute groups (p < .05). Gravity separation of cardiotomy suction blood is effective; however, it may require retention of blood for more time than is clinically acceptable during a routing coronary artery bypass graft surgery.
Kinard
M Rhett
MR
Division of Cardiovascular Perfusion, College of Health Professions, Medical University of South Carolina, 151B Rutledge Avenue, Charleston, SC 29425, USA.
Shackelford
Anthony G
AG
Sistino
Joseph J
JJ
D000704:Analysis of Variance; D000818:Animals; D002315:Cardiopulmonary Bypass / Q000379:methods*; D004620:Embolism, Fat / Q000097:blood; D006112:Gravitation; D023421:Models, Animal; D010496:Pericardium / Q000378:metabolism; D013024:Soybean Oil / Q000302:isolation & purification; D013396:Suction / Q000379:methods*; D013552:Swine; D013696:Temperature; D014280:Triglycerides / Q000097:blood* / Q000302:isolation & purification
D014280:Triglycerides; D013024:Soybean Oil
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