[Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion].
OBJECTIVE
To investigate the expression of intercellular adhesion molecular-1 (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization (FISH) and its relationship with the contusion interval.


METHODS
To make the contusion models with rats skeletal muscle. The samples were taken to extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control group after contusion respectively. FISH was performed on frozen section samples and the sections were observed using LSCM.


RESULTS
The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times the level of control group at 18 h and then increased again.


CONCLUSIONS
The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.
Pei
Ming
M
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. peiming5992@163.com
Sun
Jun-Hong
JH
Gao
Cai-Rong
CR
D000818:Animals; D003288:Contusions / Q000378:metabolism* / Q000473:pathology; D005260:Female; D049429:Forensic Pathology; D006614:Hindlimb / Q000293:injuries*; D017404:In Situ Hybridization, Fluorescence; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014945:Wound Healing
D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1