title: Induction of complement-mediated lysis of HIV-1 by a combination of HIV-specific and HLA allotype-specific antibodies.
abstract: HLA-specific antibodies generated by allo-immunization are supposed to be involved in the control of HIV infections by both the neutralizing capacity of HLA-specific antibodies (Abs) and HLA-specific Ab-dependent complement-mediated lysis (CML). We further characterized CML of HIV primary isolates induced by HLA-specific Abs. Although HIV-specific and HLA allo-type specific Abs induced only weak CML of HIV primary isolates, several combinations of HLA allo-type specific Abs with HIV-specific Abs could enhance CML significantly. Nevertheless, certain HLA-specific Abs did not improve but even inhibit CML of HIV, although the corresponding HLA molecules were present. Thus, our results emphasize a possible limitation of allo-immunization as a potential approach to induce protective immunity against HIV.
authors.forename: Alexander
authors.initials: A
authors.lastname: Hildgartner
authors.forename: Doris
authors.initials: D
authors.lastname: Wilflingseder
authors.forename: Christoph
authors.initials: C
authors.lastname: Gassner
authors.forename: Manfred P
authors.initials: MP
authors.lastname: Dierich
authors.forename: Heribert
authors.initials: H
authors.lastname: Stoiber
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Bánki
mesh_terms: D000818:Animals; D003165:Complement System Proteins / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000628:therapy / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007167:Immunotherapy / Q000379:methods; D051379:Mice
chemical_list: D015483:HIV Antibodies; D006680:HLA Antigens; D007126:Immunoglobulin Allotypes; D003165:Complement System Proteins