Localization and characteristics of DNA underreplication zone in the 75C region of intercalary heterochromatin in Drosophila melanogaster polytene chromosomes.
In Drosophila polytene chromosomes, regions of intercalary heterochromatin are scattered throughout the euchromatic arms. Here, we present data on the first fine analysis of the individual intercalary heterochromatin region, 75C1-2, located in the 3L chromosome. By using electron microscopy, we demonstrated that this region appears as three closely adjacent condensed bands. Mapping of the region on the physical map by means of the chromosomal rearrangements with known breakpoints showed that the length of the region is about 445 kb. Although it seems that the SUUR protein binds to the whole 75C1-2 region, the proximal part of the region is fully polytenized, so the DNA underreplication zone is asymmetric and located in the distal half of the region. Finally, we speculate that intercalary heterochromatin regions of Drosophila polytene chromosomes are organized into three different types with respect to the localization of the underreplication zone.
Andreyenkova
Natalya G
NG
Department of Molecular and Cellular Biology, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Kokoza
Elena B
EB
Semeshin
Valery F
VF
Belyaeva
Elena S
ES
Demakov
Sergey A
SA
Pindyurin
Alexey V
AV
Andreyeva
Eugenia N
EN
Volkova
Elena I
EI
Zhimulev
Igor F
IF
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D053903:DNA Breaks, Double-Stranded; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism* / Q000648:ultrastructure; D006570:Heterochromatin / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D020161:Physical Chromosome Mapping; D021381:Protein Transport
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006570:Heterochromatin; C464850:SuUR protein, Drosophila
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