A Proteomics Approach Highlights a Myriad of Transporters in the Arabidopsis thaliana Vacuolar Membrane.
To better understand plant vacuolar functions and identify new transporters present on the tonoplast, a proteomic work was initiated on Arabidopsis thaliana. A procedure was developed to prepare highly purified vacuoles from protoplasts isolated from Arabidopsis cell cultures, and a proteomics approach was designed to identify the protein components present in both the membrane and soluble fractions of the vacuoles. This procedure allowed the identification of 650 proteins, 2/3 of which copurify with the hydrophobic membrane fraction and 1/3 with the soluble fraction. With regard to function, only 20% of the proteins identified were previously known to be associated with vacuolar activities.
Jaquinod
Michel
M
Laboratoire d'Etude de la Dynamique des Protéomes Institut de Recherches en Technologies et Sciences pour le Vivant; Commissariat à l'Energie Atomique; Université Joseph Fourier, Grenoble France.
Villiers
Florent
F
Kieffer-Jaquinod
Sylvie
S
Hugouvieux
Véronique
V
Bruley
Christophe
C
Garin
Jérôme
J
Bourguignon
Jacques
J
Hepatology. 2002 Oct;36(4 Pt 1):819-28
12297829
Curr Biol. 2004 Nov 9;14(21):1888-96
15530389
Biochemistry. 2004 Nov 9;43(44):14189-98
15518569
Science. 2000 Dec 22;290(5500):2295-8
11125140
J Biol Chem. 2001 Aug 10;276(32):30231-44
11346655
J Biol Chem. 1998 Nov 20;273(47):31388-94
9813049
Proteomics. 2004 Feb;4(2):397-406
14760709
Trends Biochem Sci. 2005 Sep;30(9):498-505
16054367
Biochimie. 2002 May-Jun;84(5-6):391-7
12423782
Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6518-23
16618929
Trends Biochem Sci. 1999 Nov;24(11):425-7
10542406
Blood Cells Mol Dis. 2004 May-Jun;32(3):411-22
15121101
J Biol Chem. 2001 Mar 23;276(12):8648-56
11115509
EMBO J. 1996 Nov 15;15(22):6122-31
8947034
Planta. 2002 Nov;216(1):107-19
12430019
Arch Biochem Biophys. 1999 Dec 1;372(1):173-8
10562431
Plant Cell Physiol. 2004 Jun;45(6):672-83
15215502
Mol Biol Cell. 2004 Jul;15(7):3393-405
15133126
Plant Cell. 2004 Dec;16(12):3285-303
15539469