Antitumor and antimetastatic activities of Rhizoma Paridis saponins.
Rhizoma Paridis saponins (RPS) have been found to show strong antitumor activity. However, few studies have yet investigated its role on pulmonary metastasis treated with this herb. To investigate the molecular mechanisms related to metastasis, we studied RPS-treated T739 mice using histopathology, immunohistochemistry and reverse transcription polymerase chain reaction. Apoptosis was measured by TUNEL assay. As a result, RPS inhibited tumor growth by inducing apoptosis and upregulated the expression of TIMP-2 and down-regulated the level of MMP-2 and MMP-9. In conclusion, RPS is a potent anticancer agent that elicits programmed cell death and inhibits metastases in murine lung adenocarcinoma in vivo.
Man
Shuli
S
School of Pharmaceutical Science and Technology, Tianjin University, Weijin Road, Tianjin, China.
Gao
Wenyuan
W
Zhang
Yanjun
Y
Yan
Lulu
L
Ma
Chaoyi
C
Liu
Changxiao
C
Huang
Luqi
L
D000230:Adenocarcinoma / Q000378:metabolism / Q000556:secondary*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D029064:Araceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000819:agonists / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D061965:Matrix Metalloproteinase Inhibitors; D012503:Saponins; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9