MCL-1ES, a novel variant of MCL-1, associates with MCL-1L and induces mitochondrial cell death.
Myeloid cell leukemia-1 (MCL-1L) is a pro-survival member of the BCL-2 family that promotes cell survival. In this study, we identify a new splicing variant of human MCL-1 that encodes MCL-1ES (extra short). Sequence analysis indicates that this variant results from splicing within the first coding exon of MCL-1 at a non-canonical GC-AG donor-acceptor pair. The deduced sequence of MCL-1ES encodes a protein of 197 amino acids, and the PEST (proline, glutamic acid, serine, and threonine) motifs present in MCL-1L are absent. MCL-1ES interacts with MCL-1L and induces mitochondrial cell death, suggesting that alternative splicing of MCL-1 may control the fate of cells.
Kim
Jae-Hong
JH
Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Republic of Korea.
Sim
Se-Hoon
SH
Ha
Hye-Jeong
HJ
Ko
Jeong-Jae
JJ
Lee
Kangseok
K
Bae
Jeehyeon
J
D017398:Alternative Splicing; D001483:Base Sequence; D016923:Cell Death / Q000502:physiology*; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*
D064549:Myeloid Cell Leukemia Sequence 1 Protein; D020033:Protein Isoforms; D019253:Proto-Oncogene Proteins c-bcl-2