title: The NBDs that wouldn't die: A cautionary tale of the use of isolated nucleotide binding domains of ABC transporters.
abstract: COMATOSE (CTS), the plant homologue of Adrenoleukodystrophy protein, is a full length ABC transporter localized in peroxisomes. In a recent article, we reported that the two-nucleotide binding domains of CTS are not functionally equivalent in vivo. Mutations in conserved residues in the Walker A (K487A) and B (D606N) motifs of NBD1 resulted in a null phenotype, whereas identical mutations in the equivalent residues in NBD2 (K1136A and D1276N) had no detectable effect.1 In order to study the effect of these mutations on the ATPase activity of the nucleotide binding domains, we cloned and expressed the isolated NBDs as maltose binding protein (MBP) fusion proteins. We show that ATPase activity is associated with the isolated MBP-NBDs. However, mutations of amino acids located in conserved motifs did not result in striking reduction in activity despite well characterized roles in ATP binding and hydrolysis.2 We urge caution in the interpretation of results obtained from the study of isolated NBD fusions and their extrapolation to the mechanism of ATP hydrolysis in ABC transporter proteins.
authors.affiliation: Centre for Plant Sciences; University of Leeds; Leeds, UK.
authors.forename: Carine
authors.initials: C
authors.lastname: De Marcos Lousa
authors.forename: Daniela
authors.initials: D
authors.lastname: Dietrich
authors.forename: Barbara
authors.initials: B
authors.lastname: Johnson
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Baldwin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Holdsworth
authors.forename: Frederica L
authors.initials: FL
authors.lastname: Theodoulou
authors.forename: Alison
authors.initials: A
authors.lastname: Baker
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