title: Vaccination-induced IgG response to Galalpha1-3GalNAc glycan epitopes in lambs protected against Haemonchus contortus challenge infection.
abstract: Lambs vaccinated with Haemonchus contortus excretory/secretory (ES) glycoproteins in combination with the adjuvant Alhydrogel are protected against H. contortus challenge infection. Using glycan micro-array analysis we showed that serum from such vaccinated lambs contains IgG antibodies that recognise the glycan antigen Galalpha1-3GalNAc-R and GalNAcbeta1-4(Fucalpha1-3)GlcNAc-R. Our studies revealed that H. contortus glycoproteins contain Galalpha1-3Gal-R as well as significant levels of Galalpha1-3GalNAc-R, which has not been previously reported. Extracts from H. contortus adult worms contain a galactosyltransferase acting on glycan substrates with a terminal GalNAc, indicating that the worms possess the enzymatic potential to synthesise terminal Gal-GalNAc moieties. These data illustrate that glycan micro-arrays constitute a promising technology for fast and specific analysis of serum anti-glycan antibodies in vaccination studies. In addition, this approach facilitates the discovery of novel, antigenic parasite glycan antigens that may have potential for developing glycoconjugate vaccines or utilization in diagnostics.
authors.affiliation: Department of Molecular Cell Biology & Immunology, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.
authors.forename: Caroline M W
authors.initials: CM
authors.lastname: van Stijn
authors.forename: Marloes
authors.initials: M
authors.lastname: van den Broek
authors.forename: Lonneke
authors.initials: L
authors.lastname: Vervelde
authors.forename: Richard A
authors.initials: RA
authors.lastname: Alvarez
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
authors.forename: Boris
authors.initials: B
authors.lastname: Tefsen
authors.forename: Irma
authors.initials: I
authors.lastname: van Die
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D004187:Disaccharides / Q000276:immunology*; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology; D006188:Haemonchiasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D015801:Helminth Proteins / Q000008:administration & dosage / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control*; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D004187:Disaccharides; D006023:Glycoproteins; D015801:Helminth Proteins; D007074:Immunoglobulin G; D014612:Vaccines; C055075:galactosyl-(1-3)galactose
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