Effect of N-acetylcysteine on (12)C(6+) ion irradiation-induced lymphocytes DNA damages and immunity changes in mice.
The aims of present study are to estimate the biological risks to the immunity of mice exposed to heavy ion radiation and to investigate the effect of N-acetylcysteine (NAC) on (12)C(6+) ion irradiation-induced lymphocyte DNA damage. Results showed that in the brine group, the levels of lymphocyte DNA damage and MN, thymocytes G(2)/M phase arrest and apoptosis percentages (except for activity of NK cells) were up at each time point. A time-response curve for MN and DNA damage appeared in the NAC group. We found that whole-body (12)C(6+) ion irradiation at a dose of 4 Gy could: induce lymphocyte DNA double-strand breaks (DSBs); immunocytes DSBs may lead to acute effects on immunity; and 200 mg/kg NAC showed significant protection against radiation harm.
Xie
Yi
Y
Research Center for Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics, Chinese Academy of Science, Lanzhou, China.
Zhang
Hong
H
Hao
Ji-Fang
JF
Qiu
Rong
R
D000111:Acetylcysteine / Q000008:administration & dosage*; D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D020450:Heavy Ions; D007113:Immunity, Innate / Q000187:drug effects* / Q000528:radiation effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000528:radiation effects*; D051379:Mice; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D014916:Whole-Body Irradiation
D011837:Radiation-Protective Agents; D000111:Acetylcysteine