title: Antioxidant efficacy of iridoid and phenylethanoid glycosides from the medicinal plant Teucrium chamaedris in cell-free systems.
abstract: Twelve glycosides, seven iridoids and five phenylethanoids, have been isolated from leaf and root methanolic extracts of Wall Germander (Teucrium chamaedrys), a Mediterranean species historically used as a medicinal plant. Among them, three iridoid and one phenylethanoid glycosides have been isolated and characterized for the first time. All of the structures have been elucidated on the basis of their spectral data, especially 1D and 2D NMR experiments. The antioxidative properties of pure metabolites, as well as of crude organic extracts of the plant, have been analyzed on the basis of their DPPH radical scavenging capability. The antioxidant capacity in cell-free systems of the isolated metabolites was carried out by measuring their capabilities to inhibit the synthesis of thiobarbituric acid reactive species in assay media using as oxidable substrates a vegetable fat and the pentose sugar 2-deoxyribose and to prevent oxidative damage of the hydrosoluble bovine serum albumin (BSA) protein. Phenylethanoid glycosides resulted efficacious DPPH radical, while iridoid glycosides prevent massively the 2-deoxyribose and BSA oxidations in assay media.
authors.affiliation: Dipartimento di Scienze della Vita, Laboratorio di Fitochimica, Seconda Università degli Studi di, Napoli, via Vivaldi 43, I-81100 Caserta, Italy.
authors.forename: Severina
authors.initials: S
authors.lastname: Pacifico
authors.forename: Brigida
authors.initials: B
authors.lastname: D'Abrosca
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Pascarella
authors.forename: Marianna
authors.initials: M
authors.lastname: Letizia
authors.forename: Piera
authors.initials: P
authors.lastname: Uzzo
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Piscopo
authors.forename: Antonio
authors.initials: A
authors.lastname: Fiorentino
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002417:Cattle; D002474:Cell-Free System; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D039823:Iridoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008968:Molecular Conformation; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism; D031367:Teucrium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D006027:Glycosides; D039823:Iridoids; D012710:Serum Albumin, Bovine