Gold nanoparticle based plasmon resonance light-scattering method as a new approach for glycogen-biomacromolecule interactions.
A model was developed for the interactions between glycogen and biomacromolecules by gold nanoparticle plasmon resonance light-scattering method. The interactions between glycogen and biomacromolecules can alter the aggregation status of gold nanoparticles, which produced intensity changes in plasmon resonance light-scattering. This is a sensitive method to study the interactions between glycogen and biomacromolecules from nano- to micromolar level. And it is also a simple method that measurement can be carried out with a common fluorospectrometer using label-free gold nanoparticles as the transducer.
Xiang
Minghui
M
Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Xu
Xiao
X
Liu
Feng
F
Li
Na
N
Li
Ke-An
KA
D000418:Albumins / Q000737:chemistry; D000818:Animals; D001703:Biophysics / Q000379:methods; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006003:Glycogen / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008962:Models, Theoretical; D036103:Nanotechnology / Q000379:methods; D010125:Ostreidae; D012542:Scattering, Radiation; D020349:Surface Plasmon Resonance
D000418:Albumins; D046911:Macromolecular Substances; D006046:Gold; D006003:Glycogen