title: Effect of beta-glucan on immunity and survival of early stage of Anabas testudineus (Bloch).
abstract: The present experiment was carried out to study the effect of different dosages of beta-glucan suspension derived from barley on the innate immune response and disease resistance of Anabas testudineus spawns against infection caused by Aeromonas hydrophila. Four different dosages of beta-glucan suspension in phosphate buffered saline at the rate of 0, 5, 10, 15 mg l(-1) were taken and 8 days old spawn were exposed for 2 h and 3 h. The cell suspension of spawn was assayed for total protein, acid phosphatase activity, lysozyme activity, bactericidal and NBT. Further, the spawns were challenged with 3 x 10(5) cells ml(-1) of A. hydrophila and survivability percentage and immunological parameters were assayed upto day 7. On day 7, most of the immunological parameters such as lysozyme activity, bactericidal activity and NBT activity were significantly enhanced after exposing the fish to all the concentrations of beta-glucan. Challenge study indicated least mortality in the group of spawns immersed in 15 mg l(-1) beta-glucan suspension for 3 h. Thus, 3 h exposure to beta-glucan suspension could reduce the mortality and increase the immunity of A. testudineus spawns.
authors.affiliation: Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar-2, Orissa, India. basantadas@yahoo.com
authors.forename: Basanta Kumar
authors.initials: BK
authors.lastname: Das
authors.forename: Chandan
authors.initials: C
authors.lastname: Debnath
authors.forename: Pranati
authors.initials: P
authors.lastname: Patnaik
authors.forename: Dipak Kumar
authors.initials: DK
authors.lastname: Swain
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Kumar
authors.forename: Bibudhendra Kumar
authors.initials: BK
authors.lastname: Misrhra
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D016980:Aeromonas hydrophila / Q000254:growth & development*; D000818:Animals; D005393:Fish Diseases / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D009113:Muramidase / Q000378:metabolism; D010471:Perches / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism; D016019:Survival Analysis; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D047071:beta-Glucans; D013481:Superoxides; D000135:Acid Phosphatase; D009113:Muramidase