Characterization of a mumps virus nucleocapsidlike particle.
The nucleocapsid protein (NP) of mumps virus (MuV), a paramyxovirus, was coexpressed with the phosphoprotein (P) in Escherichia coli. The NP and P proteins form a soluble complex containing RNA. Under a transmission electron microscope, the NP-RNA complex appears as a nucleocapsidlike ring that has a diameter of approximately 20 nm with 13 subunits. There is a piece of single-stranded RNA with a length of 78 nucleotides in the NP-RNA ring. Shorter RNA pieces are also visible. The MuV NP protein may provide weaker protection of the RNA than the NP protein of some other negative-strand RNA viruses, reflecting the degree of NP protein association.
Cox
Robert
R
Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, Alabama 35294, USA.
Green
Todd J
TJ
Qiu
Shihong
S
Kang
Jungsoon
J
Tsao
Jun
J
Prevelige
Peter E
PE
He
Biao
B
Luo
Ming
M
D006801:Humans; D046529:Microscopy, Electron, Transmission; D046912:Multiprotein Complexes; D009109:Mumps virus / Q000378:metabolism* / Q000648:ultrastructure; D019251:Nucleocapsid / Q000378:metabolism / Q000648:ultrastructure; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D010316:Particle Size; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D012367:RNA, Viral / Q000378:metabolism / Q000648:ultrastructure; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure
D046912:Multiprotein Complexes; D019590:Nucleocapsid Proteins; D010750:Phosphoproteins; D012367:RNA, Viral
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