Assaying chromosome pairing by FISH analysis of spread Saccharomyces cerevisiae nuclei.
Fluorescent in situ hybridization (FISH) provides a powerful tool to study the localization of DNA sequences in relationship to one another. FISH has the advantage over other methods, notably use of GFP-tagged repressor/operator arrays, that an almost unlimited number of probes can be utilized without having to make new strains for each new locus one wants to study. Also, the number of sites that can be visualized at the same time is limited only by the number of fluorophores that are available and can be distinguished by the available microscope. Described here is a method for FISH analysis and its application to analysis of chromosome pairing during meiosis in S. cerevisiae.
Weiner
Beth M
BM
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
Kleckner
Nancy
N
D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics*; D023902:Chromosome Pairing / Q000502:physiology*; D015825:Chromosomes, Fungal / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008540:Meiosis / Q000235:genetics / Q000502:physiology; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D016707:Tissue Fixation / Q000379:methods*