title: Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro.
abstract: Periostin is over expressed in many epithelial malignant cancers, including lung cancer, breast cancer, ovarian cancer and colon cancer. It is related with the progression and migration of breast and ovarian cancer cells in vitro. The aim of this study was to investigate the serum level of periostin in non-small cell lung cancer (NSCLC) and its relationship with established biological and prognostic factors by enzyme-linked-immunosorbent serologic assay. We also observe the function of periostin on the proliferation and migration of human lung adenocarcinoma cell line (A549) and discuss the mechanism. The mean value for serum periostin (POSTN) was elevated in NSCLC patients (242.84 + or - 5.33 pg/ml) compared to the normal healthy volunteers (215.66 + or - 11.67 pg/ml) (p = 0.030). The serum level of periostin of NSCLC patients had no connection with gender, age, pathological type, TNM stage, lymph node status, tumor size and invasiveness. We constructed a plasmid named pEGFP-N1/POSTN expressing full-length human periostin. Transfecting the plasmid to A549 cells and periostin was efficiently expressed in transfected A549 cells. Our data showed that periostin could promote the proliferation and migration of A549 cells by inducing vimentin and N-cadherin expression and downregulating E-cadherin expression. These results strongly suggest that periostin is a novel molecular which play an important role during the progression and development of NSCLC.
authors.affiliation: Department of Respiratory Diseases, Jinling Hospital, School of Medicine, Nanjing University, 210002, Nanjing, Jiangsu Province, People's Republic of China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Hong
authors.forename: Huiming
authors.initials: H
authors.lastname: Sun
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Lv
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.forename: Jinan
authors.initials: J
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000235:genetics / Q000473:pathology*; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000097:blood*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004274:DNA, Recombinant / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology*; D008297:Male; D008648:Mesoderm / Q000378:metabolism / Q000473:pathology; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014945:Wound Healing
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D004274:DNA, Recombinant; C512835:POSTN protein, human; D012333:RNA, Messenger
references.citation: Head Neck. 2005 Oct;27(10):873-82
references.pmid: 16136586
references.citation: Cancer Res. 2002 Sep 15;62(18):5358-64
references.pmid: 12235007
references.citation: Nat Rev Cancer. 2004 Feb;4(2):118-32
references.pmid: 14964308
references.citation: Trends Mol Med. 2007 Dec;13(12):535-41
references.pmid: 17981506
references.citation: Clin Cancer Res. 2008 Nov 15;14(22):7430-7
references.pmid: 19010860
references.citation: Nat Rev Mol Cell Biol. 2006 Feb;7(2):131-42
references.pmid: 16493418
references.citation: Oncogene. 2007 Mar 29;26(14):2082-94
references.pmid: 17043657
references.citation: Cancer. 2001 Aug 15;92(4):843-8
references.pmid: 11550156
references.citation: J Cell Biol. 1998 Jul 27;142(2):557-71
references.pmid: 9679152
references.citation: Cell. 2004 Aug 6;118(3):277-9
references.pmid: 15294153
references.citation: Carcinogenesis. 2005 May;26(5):908-15
references.pmid: 15731169
references.citation: Dev Dyn. 2004 Apr;229(4):857-68
references.pmid: 15042709
references.citation: J Cell Biol. 2006 May 8;173(3):431-41
references.pmid: 16682529
references.citation: J Clin Invest. 2002 Aug;110(3):341-50
references.pmid: 12163453
references.citation: J Bone Miner Metab. 2000;18(3):132-9
references.pmid: 10783846
references.citation: J Cell Biol. 1982 Oct;95(1):333-9
references.pmid: 7142291
references.citation: Mol Cell Biol. 2004 May;24(9):3992-4003
references.pmid: 15082792
references.citation: Cell Adhes Commun. 1996 Jul;4(1):13-24
references.pmid: 8870970
references.citation: J Cell Biol. 1994 Oct;127(1):247-56
references.pmid: 7929567
references.citation: Circ Res. 2009 Jan 2;104(1):e1-7
references.pmid: 19038863
references.citation: Lung Cancer. 2002 May;36(2):115-24
references.pmid: 11955645
references.citation: Breast Cancer Res Treat. 2003 Feb;77(3):245-52
references.pmid: 12602924
references.citation: Nature. 1995 Mar 23;374(6520):327-37
references.pmid: 7885471
references.citation: J Cell Biol. 2000 Feb 7;148(3):399-404
references.pmid: 10662767
references.citation: Cancer Res. 2006 Jul 15;66(14):6928-35
references.pmid: 16849536
references.citation: J Mammary Gland Biol Neoplasia. 2003 Apr;8(2):145-58
references.pmid: 14635791
references.citation: Biochem J. 1993 Aug 15;294 ( Pt 1):271-8
references.pmid: 8363580
references.citation: Cancer Lett. 2009 Aug 28;281(2):213-9
references.pmid: 19328625
references.citation: Mol Cell Neurosci. 1996;8(2-3):99-111
references.pmid: 8918827
references.citation: Cancer Cell. 2004 Apr;5(4):329-39
references.pmid: 15093540
references.citation: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references.pmid: 15761078
references.citation: Lab Invest. 2004 Aug;84(8):999-1012
references.pmid: 15195114
references.citation: Clin Cancer Res. 2005 Dec 15;11(24 Pt 1):8686-98
references.pmid: 16361555
references.citation: Histol Histopathol. 2007 Oct;22(10):1167-74
references.pmid: 17616943
references.citation: Br J Cancer. 2007 Mar 26;96(6):986-92
references.pmid: 17325702
references.citation: Am J Pathol. 1999 Apr;154(4):981-6
references.pmid: 10233835
references.citation: J Pediatr Surg. 2002 Sep;37(9):1293-7
references.pmid: 12194119
references.citation: Toxicol Sci. 2008 Dec;106(2):364-75
references.pmid: 18796497
references.citation: J Bone Miner Res. 1999 Jul;14(7):1239-49
references.pmid: 10404027