In vivo detection of lamellocytes in Drosophila melanogaster.
Drosophila has recently become a powerful model organism for studies of innate immunity. The cellular elements of innate immunity in Drosophila, the hemocytes, have been characterized by morphological criteria, molecular markers, and cell-type-specific immunological markers. Here we suggest that an MiET1 GFP-reporter element insertion in the untranslated region of a gene (l1-atilla) - expressed in a subset of hemocytes, the lamellocytes - allows in vivo investigations of lamellocyte differentiation and facilitates genetic screens.
Honti
Viktor
V
Kurucz
Eva
E
Csordás
Gábor
G
Laurinyecz
Barbara
B
Márkus
Róbert
R
Andó
István
I
D000818:Animals; D002454:Cell Differentiation; D004251:DNA Transposable Elements / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins