Detection of multiple infections by Monocystis strains in a single earthworm host using ribosomal internal transcribed spacer sequence variation.
Monocystis sp. are sporocyst-forming apicomplexan parasites common in seminal vesicles of the earthworm Lumbricus terrestris where they may account for temporary castration. This study describes the internal transcribed spacer (ITS) region of the ribosomal cistron of Monocystis sp. This region, including ITS-1, the 5.8S ribosomal RNA gene, and ITS-2, was PCR amplified, cloned, and sequenced for Monocystis sp. isolated from the seminal vesicles of several wild-caught L. terrestris. Our analysis revealed substantial polymorphisms, also within single host organisms, indicating intra-host diversity of parasites. These genetic markers are the first that allow distinction of Monocystis sp. genotypes, opening new avenues for the study of parasite diversity within and between hosts.
Velavan
T P
TP
Department of Animal Evolutionary Ecology, University of Tübingen, Germany. velavanp@yahoo.com
Schulenburg
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H
Michiels
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NK
D000818:Animals; D016782:Apicomplexa / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics*; D014644:Genetic Variation; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S