title: Construction of bifunctional phage display for biological analysis and immunoassay.
abstract: A phage display-based bifunctional display system was developed for simple and sensitive immunoassay. The resulting bifunctional phage could simultaneously display a few single-chain variable fragment (ScFv) and many copies of the gold-binding peptide on its surface, thereby mediating antigen recognition and signal amplification. As a demonstration study, it was possible for bifunctional phage-based immunoassay to identify Bacillus anthracis spores from other Bacillus strains with detection sensitivity 10-fold higher than that of conventional phage enzyme-linked immunosorbent assay (ELISA). This protocol may be applied to build other bifunctional phage clones for broad applications (e.g., immunoassay kits, affinity biosensors, biorecognition assays).
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Guo
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Liang
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hongping
authors.initials: H
authors.lastname: Wei
authors.forename: Dong
authors.initials: D
authors.lastname: Men
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
mesh_terms: D001408:Bacillus anthracis / Q000302:isolation & purification*; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000648:ultrastructure; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism; D013171:Spores, Bacterial / Q000302:isolation & purification
chemical_list: D019151:Peptide Library; D010455:Peptides