title: The infrared spectra of Bacillus bacteria part I: vegetative Bacillus versus sporulated cells and the contributions of phospholipids to vegetative infrared spectra.
abstract: This paper highlights the distinctions between the infrared (IR) absorption spectra of vegetative versus sporulated Bacillus bacteria. It is observed that there are unique signatures clearly associated with either the sporulated or vegetative state and that vegetative cells (or cell debris) can contribute to the spore spectra. A distinct feature at approximately 1739 cm(-1) appears to be unique to vegetative cell spectra and can also be used as an indicator of vegetative cells or cell debris in the spore spectra. The data indicate that the band arises from a lipid-soluble species such as an ester or phospholipid carbonyl bond and are consistent with it being either phosphatidyl glycerol (PG) or phosphatidylethanolamine (PE), two major classes of phospholipids found in vegetative cells of Bacillus species. A companion work discusses bands associated with the sporulated state.
authors.affiliation: Pacific Northwest National Laboratory, P.O. Box 999 Richland, Washington 99354, USA. Timothy.Johnson@pnl.gov
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Johnson
authors.forename: Yin-Fong
authors.initials: YF
authors.lastname: Su
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Valentine
authors.forename: Helen W
authors.initials: HW
authors.lastname: Kreuzer-Martin
authors.forename: Karen L
authors.initials: KL
authors.lastname: Wahl
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Williams
authors.forename: Brian H
authors.initials: BH
authors.lastname: Clowers
authors.forename: David S
authors.initials: DS
authors.lastname: Wunschel
mesh_terms: D001407:Bacillus / Q000737:chemistry* / Q000166:cytology; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013171:Spores, Bacterial / Q000737:chemistry
chemical_list: D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010743:Phospholipids; C483858:phosphatidylethanolamine