MicroRNA-9 inhibits ovarian cancer cell growth through regulation of NF-kappaB1.
MicroRNAs are emerging as important regulators of cancer-related processes. Our studies show that microRNA-9 (miR-9) is downregulated in human ovarian cancer relative to normal ovary, and overexpression of miR-9 suppresses cell growth in vitro. Furthermore, the 3'-UTR of NF-kappaB1 mRNA is found to be regulated directly by miR-9, demonstrating that NF-kappaB1 is a functionally important target of miR-9 in ovarian cancer cells. When miR-9 is overexpressed in ovarian cancer cells, the mRNA and protein levels of NF-kappaB1 are both suppressed, whereas inhibition of miR-9 results in an increase in the NF-kappaB1 expression level. Ovarian cancer tissues display significantly low expression of miR-9 and a high level of NF-kappaB1 compared with normal tissues, indicating that regulation of NF-kappaB1 by miR-9 is an important mechanism for miR-9 to inhibit ovarian cancer proliferation.
Guo
Li-Min
LM
Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, China.
Pu
Yong
Y
Han
Zhe
Z
Liu
Tao
T
Li
Yi-Xuan
YX
Liu
Min
M
Li
Xin
X
Tang
Hua
H
D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000235:genetics*; D052002:NF-kappa B p50 Subunit / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D014410:Tumor Stem Cell Assay
D020413:3' Untranslated Regions; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering