title: Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods.
abstract: The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.
authors.affiliation: Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Crystiane Rodrigues Araújo
authors.initials: CR
authors.lastname: Mota
authors.forename: Karla Carvalho
authors.initials: KC
authors.lastname: Miranda
authors.forename: Janine de Aquino
authors.initials: Jde A
authors.lastname: Lemos
authors.forename: Carolina Rodrigues
authors.initials: CR
authors.lastname: Costa
authors.forename: Lúcia Kioko
authors.initials: LK
authors.lastname: Hasimoto e Souza
authors.forename: Xisto Sena
authors.initials: XS
authors.lastname: Passos
authors.forename: Hildene
authors.initials: H
authors.lastname: Meneses e Silva
authors.forename: Maria do Rosário Rodrigues
authors.initials: Mdo R
authors.lastname: Silva
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000145:classification / Q000187:drug effects*; D006801:Humans; D021261:Parasitic Sensitivity Tests / Q000379:methods
chemical_list: D000935:Antifungal Agents