title: Proteomics of mouse liver microsomes: performance of different protein separation workflows for LC-MS/MS.
abstract: The mouse liver microsome proteome was investigated using ion trap MS combined with three separation workflows including SDS-PAGE followed by reverse-phase LC of in-gel protein digestions (519 proteins identified); 2-D LC of protein digestion (1410 proteins); whole protein separation on mRP heat-stable column followed by 2-D LC of protein digestions from each fraction (3-D LC; 3703 proteins). The higher number of proteins identified in the workflow corresponded to the lesser percentage of run-to-run reproducibility. Gel-based method yielded a number of predicted membrane proteins similar to LC-based workflows.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, 119121, Russia.
authors.forename: Victor G
authors.initials: VG
authors.lastname: Zgoda
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Moshkovskii
authors.forename: Elena A
authors.initials: EA
authors.lastname: Ponomarenko
authors.forename: Timofey V
authors.initials: TV
authors.lastname: Andreewski
authors.forename: Arthur T
authors.initials: AT
authors.lastname: Kopylov
authors.forename: Olga V
authors.initials: OV
authors.lastname: Tikhonova
authors.forename: Stanislav A
authors.initials: SA
authors.lastname: Melnik
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Lisitsa
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry