title: Vitellogenin of the Chilean flounder Paralichthys adspersus as a biomarker of endocrine disruption along the marine coast of the South Pacific. Part I: induction, purification, and identification.
abstract: We sought to provide a useful indicator of the presence of endocrine-disrupting contaminants along the marine coast of the South Pacific using Chilean flounder (Paralichthys adspersus). In light of the lack of information on vitellogenin for this species, we induced, purified, and identified the plasma vitellogenin of Chilean flounder inhabiting the Chilean coast. Vitellogenin (Vg) from Chilean flounder was purified by size exclusion and ion-exchange chromatography using plasma from juvenile males induced by injecting 17beta-estradiol. The Vg was detected by SDS-PAGE and Western blot analyses using an antibody against turbot (Scophthalmus maximus) vitellogenin. These analyses revealed a protein band of 205 kDa and three minor bands of 120, 90, and 68 kDa. These proteins were identified as Vg by means of mass spectrometry (LCQ Duo ESI-IT-MS), matching sequences of tryptic peptides to known sequences for several other fish species. The matches showed the presence of vitellogenin (VgI, VgII, Vg A and Vg B) in Chilean flounder, similar to species such as mummichog (Fundulus heteroclitus), Japanese medaka (Oryzias latipes), and white perch (Morone americana). These results are discussed in terms of identifying Vg in Paralichthys adspersus with the antibody to turbot Vg. Moreover, we compare the molecular size of Vg from Chilean flounder (large) with that of other flatfish species. Finally, we discuss the potential use of this molecule as a biomarker for the presence of xeno-estrogenic compounds along the Chilean coastline.
authors.affiliation: PIMEX-ARAUCO Program, Faculty of Natural Sciences and Oceanography, University of Concepción, Concepción, Chile. mleonard@udec.cl.
authors.forename: Maritza
authors.initials: M
authors.lastname: Leonardi
authors.affiliation: PIMEX-ARAUCO Program, Faculty of Natural Sciences and Oceanography, University of Concepción, Concepción, Chile.
authors.forename: Jeanett
authors.initials: J
authors.lastname: Vera
authors.affiliation: PIMEX-ARAUCO Program, Faculty of Natural Sciences and Oceanography, University of Concepción, Concepción, Chile.|Department of Zoology, Faculty of Natural Sciences and Oceanography, University of Concepción, Concepción, Chile.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Tarifeño
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: Marcia
authors.initials: M
authors.lastname: Puchi
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: Violeta
authors.initials: V
authors.lastname: Morín
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D002677:Chile; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D052244:Endocrine Disruptors / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005412:Flatfishes / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D010140:Pacific Ocean; D013045:Species Specificity; D014819:Vitellogenins / Q000302:isolation & purification* / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D014819:Vitellogenins; D014874:Water Pollutants, Chemical
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