Construction of bifunctional phage display for biological analysis and immunoassay.
A phage display-based bifunctional display system was developed for simple and sensitive immunoassay. The resulting bifunctional phage could simultaneously display a few single-chain variable fragment (ScFv) and many copies of the gold-binding peptide on its surface, thereby mediating antigen recognition and signal amplification. As a demonstration study, it was possible for bifunctional phage-based immunoassay to identify Bacillus anthracis spores from other Bacillus strains with detection sensitivity 10-fold higher than that of conventional phage enzyme-linked immunosorbent assay (ELISA). This protocol may be applied to build other bifunctional phage clones for broad applications (e.g., immunoassay kits, affinity biosensors, biorecognition assays).
Guo
Yongchao
Y
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Liang
Xiaosheng
X
Zhou
Yafeng
Y
Zhang
Zhiping
Z
Wei
Hongping
H
Men
Dong
D
Luo
Ming
M
Zhang
Xian-En
XE
D001408:Bacillus anthracis / Q000302:isolation & purification*; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000648:ultrastructure; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism; D013171:Spores, Bacterial / Q000302:isolation & purification
D019151:Peptide Library; D010455:Peptides