title: Identification of Chlamydia trachomatis CT621, a protein delivered through the type III secretion system to the host cell cytoplasm and nucleus.
abstract: Chlamydiae are obligate intracellular bacteria, developing inside host cells within chlamydial inclusions. From these inclusions, the chlamydiae secrete proteins into the host cell cytoplasm. A pathway through which secreted proteins can be delivered is the type III secretion system (T3SS). The T3SS is common to several gram-negative bacteria and the secreted proteins serve a variety of functions often related to the modulation of host signalling. To identify new potentially secreted proteins, the cytoplasm was extracted from Chlamydia trachomatis L2-infected HeLa cells, and two-dimensional polyacrylamide gel electrophoresis profiles of [(35)S]-labelled chlamydial proteins from this extract were compared with profiles of chlamydial proteins from the lysate of infected cells. In this way, CT621 was identified. CT621 is a member of a family of proteins containing a domain of unknown function DUF582 that is only found within the genus Chlamydia. Immunofluorescence microscopy and immunoblotting demonstrated that CT621 is secreted late in the chlamydial developmental cycle and that it is the first chlamydial protein found to be localized within both the host cell cytoplasm and the nucleus. To determine whether CT621 is secreted through the T3SS, an inhibitor of this apparatus was added to the infection medium, resulting in retention of the protein inside the chlamydiae. Hence, the so far uncharacterized CT621 is a new type III secretion effector protein.
authors.affiliation: Department of Medical Microbiology and Immunology, Aarhus University, Aarhus C, Denmark.
authors.forename: Anne-Sofie
authors.initials: AS
authors.lastname: Hobolt-Pedersen
authors.forename: Gunna
authors.initials: G
authors.lastname: Christiansen
authors.forename: Evy
authors.initials: E
authors.lastname: Timmerman
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Svend
authors.initials: S
authors.lastname: Birkelund
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry*; D002692:Chlamydia trachomatis / Q000472:pathogenicity*; D003593:Cytoplasm / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D021381:Protein Transport; D020543:Proteome / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods; D013462:Sulfur Radioisotopes / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D013462:Sulfur Radioisotopes; D037521:Virulence Factors
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