title: Full-length sequence of mouse acupuncture-induced 1-L (aig1l) gene including its transcriptional start site.
abstract: We have been investigating the molecular efficacy of electroacupuncture (EA), which is one type of acupuncture therapy. In our previous molecular biological study of acupuncture, we found an EA-induced gene, named acupuncture-induced 1-L (Aig1l), in mouse skeletal muscle. The aims of this study consisted of identification of the full-length cDNA sequence of Aig1l including the transcriptional start site, determination of the tissue distribution of Aig1l and analysis of the effect of EA on Aig1l gene expression. We determined the complete cDNA sequence including the transcriptional start site via cDNA cloning with the cap site hunting method. We then analyzed the tissue distribution of Aig1l by means of northern blot analysis and real-time quantitative polymerase chain reaction. We used the semiquantitative reverse transcriptase-polymerase chain reaction to examine the effect of EA on Aig1l gene expression. Our results showed that the complete cDNA sequence of Aig1l was 6073 bp long, and the putative protein consisted of 962 amino acids. All seven tissues that we analyzed expressed the Aig1l gene. In skeletal muscle, EA induced expression of the Aig1l gene, with high expression observed after 3 hours of EA. Our findings thus suggest that the Aig1l gene may play a key role in the molecular mechanisms of EA efficacy.
authors.affiliation: Laboratory for Applied Genome Science and Bioinformatics, Clinical Genome Informatics Centre, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Ohta
authors.forename: Aki
authors.initials: A
authors.lastname: Sugano
authors.forename: Shuji
authors.initials: S
authors.lastname: Goto
authors.forename: Surini
authors.initials: S
authors.lastname: Yusoff
authors.forename: Yushi
authors.initials: Y
authors.lastname: Hirota
authors.forename: Kotaro
authors.initials: K
authors.lastname: Funakoshi
authors.forename: Kenji
authors.initials: K
authors.lastname: Miura
authors.forename: Eiichi
authors.initials: E
authors.lastname: Maeda
authors.forename: Nobuo
authors.initials: N
authors.lastname: Takaoka
authors.forename: Nobuko
authors.initials: N
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishizuka
authors.forename: Naoki
authors.initials: N
authors.lastname: Arizono
authors.forename: Hisahide
authors.initials: H
authors.lastname: Nishio
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takaoka
references.citation: Mol Cell Endocrinol. 2007 May 30;270(1-2):1-7
references.pmid: 17462816
references.citation: Biochem Biophys Res Commun. 1995 Nov 2;216(1):382-9
references.pmid: 7488116
references.citation: Cell. 1988 May 20;53(4):505-18
references.pmid: 3286010
references.citation: Oncogene. 2000 Dec 14;19(54):6251-60
references.pmid: 11175339
references.citation: Nat Rev Genet. 2006 Mar;7(3):200-10
references.pmid: 16485019
references.citation: Genes Dev. 2001 Jul 1;15(13):1625-30
references.pmid: 11445537
references.citation: Neuron. 2007 Nov 21;56(4):621-39
references.pmid: 18031681
references.citation: J Biol Chem. 2002 May 17;277(20):18069-76
references.pmid: 11886873
references.citation: Gene. 1994 Jan 28;138(1-2):171-4
references.pmid: 8125298
references.citation: Trends Immunol. 2004 Jul;25(7):368-73
references.pmid: 15207504
references.citation: Physiol Genomics. 2007 Jul 18;30(2):102-10
references.pmid: 17341691
references.citation: Mol Cell Biol. 2005 Jul;25(14):6005-20
references.pmid: 15988015
references.citation: Blood. 2005 Dec 15;106(13):4139-45
references.pmid: 16141351
references.citation: J Mol Biol. 1993 May 20;231(2):539-45
references.pmid: 8510165
references.citation: J Biochem Mol Biol. 2002 Jul 31;35(4):420-7
references.pmid: 12297003
references.citation: Cell. 2006 Jan 13;124(1):147-59
references.pmid: 16413488
references.citation: J Neuroimmunol. 2005 Nov;168(1-2):144-53
references.pmid: 16154208
references.citation: Trends Cardiovasc Med. 2002 Jan;12(1):13-9
references.pmid: 11796239
references.citation: J Biol Chem. 2006 Mar 17;281(11):7406-12
references.pmid: 16407223
references.citation: J Physiol Sci. 2006 Dec;56(6):425-31
references.pmid: 17083754
references.citation: FEBS J. 2005 Nov;272(22):5723-41
references.pmid: 16279938
references.citation: Nature. 2007 Jun 7;447(7145):661-78
references.pmid: 17554300
references.citation: Biochem Biophys Res Commun. 2004 Jul 30;320(3):1043-50
references.pmid: 15240153
references.citation: Cell. 1985 Dec;43(3 Pt 2):567-81
references.pmid: 3935325
references.citation: Mol Cell Biol. 2006 May;26(10):3942-54
references.pmid: 16648487
references.citation: Nature. 2004 Jan 29;427(6973):451-7
references.pmid: 14749834
references.citation: J Cell Sci. 2003 May 15;116(Pt 10):1863-73
references.pmid: 12692188
references.citation: FEBS Lett. 2003 Apr 10;540(1-3):21-5
references.pmid: 12681477
references.citation: Brain Res Mol Brain Res. 1995 Feb;28(2):201-10
references.pmid: 7723619
references.citation: J Mol Biol. 1991 Jun 20;219(4):717-25
references.pmid: 1829116
references.citation: Biol Reprod. 2005 Apr;72(4):898-907
references.pmid: 15601926