[Human mesenchymal stem cells modified by hepatocyte growth factor gene promote chicken embryonic angiogenesis].
This study was purposed to investigate the angiogenesis-promoting activities of human mesenchymal stem cells (hMSCs) modified by hepatocyte growth factor (HGF) and the underlying mechanisms. The hMSCs were transfected by recombinant adenoviral vector carrying human HGF gene and seeded onto the chicken chorioallantoic membrane. Three days later, the number of blood vessels was counted and their angiogenic response was compared with those of hMSCs of same generation, recombinant basic fibroblast growth factor (bFGF) and alpha-MEM as control. The expression levels of bFGF, VEGF, angiopoietin-1 and angiopoietin-2 were evaluated by RT-PCR assay. The results showed that gene-modified hMSCs exhibited greatest activity to promote angiogenesis while the angiogenic response was nearly same between groups treated by hMSCs and bFGF, all of which were significantly higher than that observed in control (p < 0.01). RT-PCR analysis revealed that hMSCs constitutively expressed multiple angiogenesis-associated growth factors and their levels seemed up-regulated by HGF gene transfer. It is concluded that HGF gene-modified hMSCs show a potent angiogenesis-promoting function and may be useful in the treatment of ischemic disorders.
Liu
Zi-Kang
ZK
Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei 230032, Anhui Province, China.
Jin
Ji-De
JD
He
Zi-Ming
ZM
Qin
Yi-De
YD
Guo
Zi-Kuan
ZK
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D006801:Humans; D059630:Mesenchymal Stem Cells; D018919:Neovascularization, Physiologic / Q000235:genetics*; D014162:Transfection
D017228:Hepatocyte Growth Factor