Effects of antifungal agents in sap activity of Candida albicans isolates.
Some antifungal agents have shown to exert effects on expression of virulent factors of Candida as the production of secretory aspartyl proteinase (Sap). In this study, we sought to determine and to compare the influence of fluconazole and voriconazole in proteinase activity of this microorganism. Thirty-one isolates obtained from oral mucosa of human immunodeficiency virus positive (HIV) patients were used in this study. The minimal inhibitory concentrations (MIC) of fluconazole and voriconazole were determined using the broth microdilution method with RPMI 1640 medium and with yeast carbon base-bovine serum albumin (YCB-BSA) medium. The Sap activity following by digestion of BSA as substrate was determined for four Candida albicans strains arbitrarily chosen according to susceptibility (susceptible or resistant) to fluconazole or voriconazole. Besides, the SAP1 to SAP7 genes were screened by PCR for the same isolates that were determined by the Sap activity. In vitro susceptibility testing using the two media presented similar MIC values. Increased Sap activity was observed in resistant isolates on presence of drugs, but the Sap activity by susceptible isolates to azoles showed different behavior on the presence of drug. We detected the presence of SAP1 to SAP7 genes from all susceptible or resistant C. albicans isolates. The present study provides important data about the proteinase activity and the presence of genes of SAP family in fluconazole and voriconazole susceptible or resistant C. albicans isolates.
Costa
Carolina Rodrigues
CR
Instituto de Patologia Tropical e Saúde Pública da, Universidade Federal de Goiás, Av. Delenda Resende de Melo S/N, Goiânia, Goiás, Brazil.
Jesuíno
Rosália Santos Amorim
RS
de Aquino Lemos
Janine
J
de Fátima Lisboa Fernandes
Orionalda
O
Hasimoto e Souza
Lúcia Kioko
LK
Passos
Xisto Sena
XS
do Rosário Rodrigues Silva
Maria
M
D000935:Antifungal Agents / Q000494:pharmacology*; D057055:Aspartic Acid Proteases / Q000037:antagonists & inhibitors* / Q000235:genetics; D002176:Candida albicans / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D015658:HIV Infections / Q000150:complications; D006801:Humans; D008826:Microbial Sensitivity Tests; D009061:Mouth Mucosa / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011743:Pyrimidines / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
D000935:Antifungal Agents; D003470:Culture Media; D004271:DNA, Fungal; D011743:Pyrimidines; D014230:Triazoles; D015725:Fluconazole; D057055:Aspartic Acid Proteases; D065819:Voriconazole
Mol Microbiol. 1998 Jul;29(2):605-15
9720876
Genetics. 1999 May;152(1):167-78
10224251
J Gen Microbiol. 1987 Oct;133(10):2817-24
3329678
Clin Microbiol Infect. 2004 Mar;10 Suppl 1:86-95
14748805
Mycoses. 2000;43 Suppl 2:17-21
11291571
FEMS Yeast Res. 2003 Jun;3(4):405-15
12748052
Clin Infect Dis. 2005 Aug 15;41(4):521-6
16028162
Mycoses. 2008;51 Suppl 1:7-18
18471156
Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14907-12
12397174
Mycoses. 2005 Nov;48(6):365-77
16262871
Diagn Microbiol Infect Dis. 2000 Jan;36(1):13-8
10744363
Mycopathologia. 2006 Apr;161(4):219-23
16552484
Nihon Ishinkin Gakkai Zasshi. 1998;39(2):73-8
9580031
Antimicrob Agents Chemother. 2007 Aug;51(8):2985-7
17517840
Mycopathologia. 2005 Oct;160(3):191-200
16205967
J Clin Microbiol. 2006 Mar;44(3):819-26
16517860
Antimicrob Agents Chemother. 2000 May;44(5):1200-8
10770752
Cell Microbiol. 2004 Oct;6(10):915-26
15339267
Microbiol Mol Biol Rev. 2003 Sep;67(3):400-28, table of contents
12966142
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Mar;91(3):317-21
11250629
J Clin Microbiol. 2003 Apr;41(4):1440-6
12682127
J Investig Dermatol Symp Proc. 2001 Dec;6(3):188-96
11924826
Folia Microbiol (Praha). 2005;50(5):409-13
16475500
Mycoses. 2007 Sep;50(5):383-90
17714358
Infect Immun. 1999 May;67(5):2482-90
10225911
Med Mycol. 2001 Aug;39(4):303-13
11556759