Stage-specific susceptibility of human erythroblasts to Plasmodium falciparum malaria infection.
Malaria parasites are known to invade and develop in erythrocytes and reticulocytes, but little is known about their infection of nucleated erythroid precursors. We used an in vitro cell system that progressed through basophilic, polychromatic, orthochromatic, and reticulocyte stages to mature erythrocytes. We show that orthochromatic cells are the earliest stages that may be invaded by Plasmodium falciparum, the causative agent of fatal human malaria. Susceptibility to invasion is distinct from intracellular survival and occurs at a time of extensive erythroid remodeling. Together these data suggest that the potential for complexity of host interactions involved in infection may be vastly greater than hitherto realized.
Tamez
Pamela A
PA
Center for Rare and Neglected Diseases, University of Notre Dame, South Bend, IN 46556, USA. ptamez@nd.edu
Liu
Hui
H
Fernandez-Pol
Sebastian
S
Haldar
Kasturi
K
Wickrema
Amittha
A
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D004900:Erythroblasts / Q000469:parasitology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions; D006801:Humans; D016778:Malaria, Falciparum / Q000209:etiology / Q000469:parasitology*; D020411:Oligonucleotide Array Sequence Analysis; D010963:Plasmodium falciparum / Q000254:growth & development / Q000472:pathogenicity*; D012156:Reticulocytes / Q000469:parasitology*
D015415:Biomarkers
Blood. 1997 Jul 15;90(2):630-40
9226163
Br J Haematol. 2008 May;141(3):276-86
18410566
J Biol Chem. 2008 Mar 14;283(11):6997-7006
18174176
J Cell Physiol. 1994 Sep;160(3):417-26
8077279
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):31-6
11134512
Ciba Found Symp. 1983;94:174-95
6341001
Int J Parasitol. 2007 Dec;37(14):1551-7
17610880
Blood. 1992 Oct 15;80(8):1940-9
1391953
Nature. 2005 Mar 10;434(7030):214-7
15759000
Br J Haematol. 1987 Jul;66(3):295-306
3304391