title: Subcutaneous graft of D1 mouse mesenchymal stem cells leads to the formation of a bone-like structure.
abstract: Mesenchymal stem cells (MSC) are capable of both self-renewal and multi-lineage differentiation into mesoderm-type cells such as osteoblasts, chondrocytes, adipocytes and myocytes. Together the multipotent nature of MSCs and the facility to expand them in vitro make these cells ideal resources for regenerative medicine, particularly for bone reconstruction, and therefore research efforts focused on defining efficient protocols for directing their differentiation into the requisite lineage. Despite much progress in identifying mechanisms and factors that direct and control in vitro osteogenic differentiation of MSCs, a rapid and simple model to evaluate in vivo tissue formation is still lacking. Here, we describe the unique capacity of the murine bone marrow-derived D1 MSC cell line, which differentiates in vitro into at least three cell lineages, to form in vivo a structure resembling bone. This bone-like structure was obtained after subcutaneous grafting of D1 cells into immunocompetent mice without the need of neither an osteogenic factor nor scaffold material. These data allow us to propose this cell model as a tool for exploring in vivo the mechanisms and/or factors that govern and potentially regulate osteogenesis.
authors.affiliation: CNRS UMR 8527, Institut Pasteur de Lille, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Juffroy
authors.forename: Danièle
authors.initials: D
authors.lastname: Noël
authors.forename: Anne
authors.initials: A
authors.lastname: Delanoye
authors.forename: Odile
authors.initials: O
authors.lastname: Viltart
authors.forename: Isabelle
authors.initials: I
authors.lastname: Wolowczuk
authors.forename: Claudie
authors.initials: C
authors.lastname: Verwaerde
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D005260:Female; D006651:Histocytochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016513:Mice, SCID; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D013047:Specific Pathogen-Free Organisms