title: y Human herpesvirus 6 envelope components enriched in lipid rafts: evidence for virion-associated lipid rafts.
abstract: In general, enveloped viruses are highly dependent on their lipid envelope for entry into host cells. Here, we demonstrated that during the course of virus maturation, a significant proportion of human herpesvirus 6 (HHV-6) envelope proteins were selectively concentrated in the detergent-resistant glycosphingolipid- and cholesterol-rich membranes (rafts) in HHV-6-infected cells. In addition, the ganglioside GM1, which is known to partition preferentially into lipid rafts, was detected in purified virions, along with viral envelope glycoproteins, gH, gL, gB, gQ1, gQ2 and gO indicating that at least one raft component was included in the viral particle during the assembly process.
authors.affiliation: Division of Biomedical Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan. akawabata@nibio.go.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawabata
authors.forename: Huamin
authors.initials: H
authors.lastname: Tang
authors.forename: Honglan
authors.initials: H
authors.lastname: Huang
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D015654:Herpesvirus 6, Human / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008563:Membrane Lipids / Q000032:analysis / Q000378:metabolism*; D019349:Roseolovirus Infections / Q000378:metabolism* / Q000821:virology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008563:Membrane Lipids; D014759:Viral Envelope Proteins
references.citation: Lancet. 1988 May 14;1(8594):1065-7
references.pmid: 2896909
references.citation: J Virol. 2003 May;77(9):5339-51
references.pmid: 12692236
references.citation: Traffic. 2008 Sep;9(10):1728-42
references.pmid: 18637904
references.citation: Virology. 2002 Sep 30;301(2):212-25
references.pmid: 12359424
references.citation: Mol Biol Cell. 2001 Jun;12(6):1819-33
references.pmid: 11408588
references.citation: J Biol Chem. 1998 Jan 9;273(2):1150-7
references.pmid: 9422781
references.citation: Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13925-30
references.pmid: 11717449
references.citation: Virology. 2008 Sep 1;378(2):265-71
references.pmid: 18621409
references.citation: J Biol Chem. 2003 Dec 19;278(51):51920-7
references.pmid: 14506277
references.citation: J Virol. 2000 Jan;74(1):305-11
references.pmid: 10590118
references.citation: J Virol. 2004 May;78(9):4609-16
references.pmid: 15078943
references.citation: J Virol. 2003 Sep;77(17):9542-52
references.pmid: 12915568
references.citation: J Virol. 2006 Nov;80(21):10652-62
references.pmid: 17041223
references.citation: J Virol. 2000 May;74(10):4634-44
references.pmid: 10775599
references.citation: J Virol. 2002 Jul;76(13):6718-28
references.pmid: 12050385
references.citation: J Gen Virol. 2002 Apr;83(Pt 4):847-854
references.pmid: 11907335
references.citation: Cell Microbiol. 2009 Jul;11(7):1001-6
references.pmid: 19290911
references.citation: Science. 1986 Oct 31;234(4776):596-601
references.pmid: 2876520
references.citation: J Virol. 2004 Aug;78(15):7969-83
references.pmid: 15254169
references.citation: Immunity. 2001 Jun;14(6):669-80
references.pmid: 11420038
references.citation: J Virol. 2000 Nov;74(21):9911-5
references.pmid: 11024118
references.citation: J Biol Chem. 1999 Jan 22;274(4):2038-44
references.pmid: 9890962
references.citation: J Virol. 2001 Aug;75(15):7175-83
references.pmid: 11435598
references.citation: J Virol. 2000 Apr;74(7):3264-72
references.pmid: 10708443
references.citation: Nat Rev Mol Cell Biol. 2003 Apr;4(4):309-19
references.pmid: 12671653
references.citation: J Virol. 2003 Feb;77(3):2038-45
references.pmid: 12525638
references.citation: Nature. 1997 Jun 5;387(6633):569-72
references.pmid: 9177342
references.citation: J Virol. 2004 May;78(10):5279-87
references.pmid: 15113909