title: Comparison of a fluoroquinolone surface plasmon resonance biosensor screening assay with established methods.
abstract: The performance of a previously developed immunochemical biosensor screening method for fluoroquinolone (FQ) antibiotics in poultry muscle, fish and egg was compared with established methods. Blank sample material of the target matrices was individually spiked with the FQs at half maximum residue levels. Homogeneity of the test materials was confirmed by liquid chromatography-mass spectrometry (LC-MS/MS). Identical sets of spiked samples as well as incurred samples from a previous feeding experiment were sent to three independent laboratories and analysed by LC-MS/MS, a microbiological assay and the new biosensor assay. The new method correctly identified all contaminated samples and demonstrated advantages in sensitivity and analysis time compared to the microbiological screening assay.
authors.affiliation: RIKILT-Institute of Food Safety, Wageningen, The Netherlands. stefan.weigel@wur.nl
authors.forename: Stefan
authors.initials: S
authors.lastname: Weigel
authors.forename: Mariel G
authors.initials: MG
authors.lastname: Pikkemaat
authors.forename: J W Alexander
authors.initials: JW
authors.lastname: Elferink
authors.forename: Patrick P J
authors.initials: PP
authors.lastname: Mulder
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Huet
authors.forename: Philippe
authors.initials: P
authors.lastname: Delahaut
authors.forename: Sonja
authors.initials: S
authors.lastname: Schittko
authors.forename: Ruth
authors.initials: R
authors.lastname: Flerus
authors.forename: Michel
authors.initials: M
authors.lastname: Nielen
mesh_terms: D015374:Biosensing Techniques; D002853:Chromatography, Liquid; D024841:Fluoroquinolones / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D053719:Tandem Mass Spectrometry
chemical_list: D024841:Fluoroquinolones