title: Genetic diversity of sapovirus infections in Danish children 2005-2007.
abstract: BACKGROUND
  Sapoviruses are known to cause gastroenteritis mainly in young children.
  
  
  OBJECTIVE
  To establish a collection of sapoviruses and to gain knowledge about the genetic diversity and epidemiology of sapoviruses circulating in children in Denmark.
  
  
  METHODS
  During a 24-month period in 2005-2007 samples from 1104 children, aged 0-3 years, submitted for acute gastroenteritis diagnostics, were analysed for sapoviruses by real-time PCR. Genotyping of positive findings was carried out by sequencing part of the capsid gene, and in several cases also part of the polymerase gene.
  
  
  RESULTS
  Sapoviruses were detected in stool samples from 97 children (9%), with the highest prevalence in the 7-18 months age group. Viruses from three genogroups and seven genotypes were found. Genotype I.1 was demonstrated in half of the positive samples and was observed throughout the study period. The less common types seemed to appear during shorter periods, often in succession of each other. Positive samples were detected throughout the study period. The only months, in both years studied, with high proportions of positive samples were September, November and February.
  
  
  CONCLUSIONS
  Sapoviruses were commonly found in children with gastroenteritis in Denmark. No clear seasonal pattern could be seen. Genotype I.1 was clearly the most common genotype found, but several other genotypes circulated during shorter periods.
authors.affiliation: Department of Virology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark.
authors.forename: Christina K
authors.initials: CK
authors.lastname: Johnsen
authors.forename: Sofie
authors.initials: S
authors.lastname: Midgley
authors.forename: Blenda
authors.initials: B
authors.lastname: Böttiger
mesh_terms: D017250:Caliciviridae Infections / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D003718:Denmark; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D015995:Prevalence; D011446:Prospective Studies; D029341:Sapovirus / Q000235:genetics*
chemical_list: D036022:Capsid Proteins