KLOTHO gene polymorphisms G-395A and C1818T are associated with low-density lipoprotein cholesterol and uric acid in Japanese hemodialysis patients.
OBJECTIVE
This study aimed to investigate the association of the KLOTHO gene single nucleotide polymorphisms (SNPs), G-395A and C1818T, with various laboratory data in 219 Japanese hemodialysis (HD) patients.


METHODS
The genotyping of G-395A in the promoter region and C1818T in exon 4 was performed using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay.


RESULTS
In HD patients, the allele frequencies of G-395A were 0.847 for the G allele and 0.153 for the A allele and those of the C1818T were 0.829 for the C allele and 0.171 for the T allele. There were no significant differences in allele frequencies of G-395A and those of the C1818T between HD patients and healthy subjects. Multivariate analysis adjusted for age and duration on HD demonstrated that uric acid was significantly high in A allele carriers of G-395A compared with GG genotype in all and female patients. Low-density lipoprotein cholesterol was significantly low in T allele carriers of C1818T compared with CC genotype in all and male patients.


CONCLUSIONS
KLOTHO gene SNPs G-395A and C1818T are associated with low-density lipoprotein cholesterol and uric acid in HD patients.
Shimoyama
Yasuhiko
Y
Department of Advanced Medicine for Uremia, Nagoya University School of Medicine, Nagoya, Japan.
Taki
Kentaro
K
Mitsuda
Yoko
Y
Tsuruta
Yoshinari
Y
Hamajima
Nobuyuki
N
Niwa
Toshimitsu
T
D000368:Aged; D044466:Asian People / Q000235:genetics* / Q000706:statistics & numerical data; D008078:Cholesterol, LDL / Q000097:blood*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology; D005838:Genotype; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D015994:Incidence; D007564:Japan / Q000453:epidemiology; D007676:Kidney Failure, Chronic / Q000208:ethnology* / Q000235:genetics* / Q000628:therapy; D000090265:Klotho Proteins; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D006435:Renal Dialysis; D014527:Uric Acid / Q000097:blood*
D008078:Cholesterol, LDL; D014527:Uric Acid; D005966:Glucuronidase; D000090265:Klotho Proteins