Cyclic AMP regulates the biosynthesis of cellobiohydrolase in Cellulomonas flavigena growing in sugar cane bagasse.
Cellulomonas flavigena produces a battery of cellulase components that act concertedly to degrade cellulose. The addition of cAMP to repressed C. flavigena cultures released catabolic repression, while addition of cAMP to induced C. flavigena cultures led to a cellobiohydrolase hyperproduction. Exogenous cAMP showed positive regulation on cellobiohydrolase production in C. flavigena grown on sugar cane bagasse. A C. flavigena cellobiohydrolase gene was cloned (named celA), which coded for a 71- kDa enzyme. Upstream, a repressor celR1, identified as a 38 kDa protein, was monitored by use of polyclonal antibodies.
Herrera-Herrera
Jesús Antonio
JA
Department of Biotechnology and Bioengineering CINVESTAV-IPN, Av. IPN 2508, México, DF, Mexico.
Pérez-Avalos
Odilia
O
Salgado
Luis M
LM
Ponce-Noyola
Teresa
T
D001426:Bacterial Proteins / Q000096:biosynthesis; D040143:Cellulomonas / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D012097:Repressor Proteins / Q000235:genetics; D031786:Saccharum / Q000737:chemistry
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; D002482:Cellulose; C027433:bagasse; D000242:Cyclic AMP; D043366:Cellulose 1,4-beta-Cellobiosidase