Cytokine array comparisons of plasma from cycling fertile women on cycle day 5 and ovulation.
OBJECTIVE
To identify plasma immuno-regulatory molecules up or down regulated between the follicular phase and ovulation of the human menstrual cycle.


METHODS
RayBio cytokine arrays were used to screen 174 immuno-regulatory molecules in plasma collected during the follicular phase at menstrual cycle day 5 and at ovulation from five healthy, non-smoking, fertile women of reproductive age not using hormonal contraception.


RESULTS
A total of 23 differentially expressed molecules were found: 10 molecules were differentially up-regulated and 13 down-regulated at ovulation compared with that at the follicular phase (alpha = 0.05, false discovery rate of 0.45).


CONCLUSIONS
Circulating immuno-regulatory molecules fluctuate over the menstrual cycle in healthy women. The combination of differentially expressed molecules suggests roles in cyclical regulation of angiogenesis and immune cell trafficking.
Hatta
Kota
K
Department of Microbiology and Immunology, Queen's University, Kingston, ON, Canada. 7kh17@queensu.ca
Bilinski
Michael J
MJ
Haladyn
J Kimberly
JK
Roy
Jessica J
JJ
Horrocks
Julie
J
van den Heuvel
Marianne J
MJ
Han
Victor K
VK
Croy
B Anne
BA
D016207:Cytokines / Q000097:blood* / Q000235:genetics / Q000276:immunology; D016208:Databases, Factual; D015536:Down-Regulation; D005260:Female; D046910:Fertile Period; D006801:Humans; D010060:Ovulation; D015854:Up-Regulation
D016207:Cytokines
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