[Bioinformatics scan of factors with inhibitory effect on lrp16 gene expression].
The main purpose of the this study was to find the candidate cis-elements in negative regulation region throngh analysing the DNA sequences of lrp16 gene promoter so as to provide the experimental basis for screening drugs with inhibitory effect on lrp16 gene expression. The open reading frame (ORF) sequences in uncoding DNA and mRNA sequences of 5' flanking region in lrp16 gene were cloned by the data in GeneBank and Internet; the possibly existing cis-element in thsi region was searched in databank of human transcriptional factor by using TESS and Genomax online promoter analysis software; the drugs related to inhibition of lrp16 gene expression were screened by using SAGE and GEO databank. The results showed that there were many cis-elements in the negative regulation region, including T-Ag, PU.1, c-Ets, XPF-1, P2 alphaA, IL6-6RE and RAR. In cultured cell lines, hormone or its inhibitor such as corticosteroid, tamoxifen, forskolin, phenylephrine, inflammatory factors such as IFNgamma and TNFalpha, and chemotherapeutics 5-fluorouracil could down-regulate the lrp16 gene expression as compared with absent ones. It is concluded that cis-elements including T-Ag, PU.1, c-Ets, XPF-1, P2 alphaA, IL6-6RE and RAR may inhibit lrp16 expression and hormone or its inhibitor such as corticosteroid, tamoxifen, forskolin, phenylephrine, inflammatory factors such as IL6, IFNgamma and TNFalpha, and chemotherapeutics 5-fluorouracil may participate in the regulation of lrp16 gene expression in negative manner.
Chi
Xiao-Hua
XH
Department of Pharmacy, 262th Hospital of PLA, Beijing 100800, China.
Liu
Li-Hong
LH
Lu
Xue-Chun
XC
Yang
Bo
B
Dong
Meng
M
D002265:Carboxylic Ester Hydrolases; D002460:Cell Line; D019295:Computational Biology; D005786:Gene Expression Regulation; D006801:Humans; D009363:Neoplasm Proteins / Q000187:drug effects* / Q000235:genetics*; D016366:Open Reading Frames; D050436:Regulatory Elements, Transcriptional
D009363:Neoplasm Proteins; D002265:Carboxylic Ester Hydrolases; C476832:MACROD1 protein, human