title: Characterization of a mumps virus nucleocapsidlike particle.
abstract: The nucleocapsid protein (NP) of mumps virus (MuV), a paramyxovirus, was coexpressed with the phosphoprotein (P) in Escherichia coli. The NP and P proteins form a soluble complex containing RNA. Under a transmission electron microscope, the NP-RNA complex appears as a nucleocapsidlike ring that has a diameter of approximately 20 nm with 13 subunits. There is a piece of single-stranded RNA with a length of 78 nucleotides in the NP-RNA ring. Shorter RNA pieces are also visible. The MuV NP protein may provide weaker protection of the RNA than the NP protein of some other negative-strand RNA viruses, reflecting the degree of NP protein association.
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, Alabama 35294, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Cox
authors.forename: Todd J
authors.initials: TJ
authors.lastname: Green
authors.forename: Shihong
authors.initials: S
authors.lastname: Qiu
authors.forename: Jungsoon
authors.initials: J
authors.lastname: Kang
authors.forename: Jun
authors.initials: J
authors.lastname: Tsao
authors.forename: Peter E
authors.initials: PE
authors.lastname: Prevelige
authors.forename: Biao
authors.initials: B
authors.lastname: He
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
mesh_terms: D006801:Humans; D046529:Microscopy, Electron, Transmission; D046912:Multiprotein Complexes; D009109:Mumps virus / Q000378:metabolism* / Q000648:ultrastructure; D019251:Nucleocapsid / Q000378:metabolism / Q000648:ultrastructure; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D010316:Particle Size; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D012367:RNA, Viral / Q000378:metabolism / Q000648:ultrastructure; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D046912:Multiprotein Complexes; D019590:Nucleocapsid Proteins; D010750:Phosphoproteins; D012367:RNA, Viral
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