Immune responses of human immature dendritic cells can be modulated by the recombinant Aspergillus fumigatus antigen Aspf1.
Invasive aspergillosis is a significant cause of morbidity and mortality in patients after stem cell transplantation, in solid organ transplant recipients, and in patients with hematological malignancies. The interactions between human immature dendritic cells (iDCs) and Aspergillus fumigatus antigens are widely uncharacterized. We analyzed the immune response of iDCs to different recombinant A. fumigatus antigens (Aspf1 and Crf1). One of these antigens, the 18-kDa RNase Aspf1, triggered the increased level of expression of genes encoding proinflammatory cytokines and chemokines, and augmented the activation of NFkappaB and the apoptosis of iDCs. Furthermore, by fluorescence microscopy, we could demonstrate that in the first 3 h a major portion of Aspf1 accumulates on the cell surface. Finally, we could show an increased segregation of cytokines and chemokines after the stimulation of iDCs by an Aspf1 deletion mutant strain of A. fumigatus.
Ok
Michael
M
Universität Würzburg, Medizinische Klinik & Poliklinik II, Institut für Hygiene & Mikrobiologie, Würzburg, Germany.
Latgé
Jean Paul
JP
Baeuerlein
Carina
C
Ebel
Frank
F
Mezger
Markus
M
Topp
Max
M
Kurzai
Oliver
O
Killian
Doreen
D
Kapp
Markus
M
Grigoleit
Goetz-Ulrich
GU
Sennefelder
Helga
H
Arroyo
Javier
J
Einsele
Hermann
H
Loeffler
Juergen
J
D000485:Allergens / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017951:Antigen Presentation; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000235:genetics; D052179:Antigens, Plant; D017209:Apoptosis; D001228:Aspergillosis / Q000209:etiology / Q000276:immunology / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D001483:Base Sequence; D002454:Cell Differentiation; D018920:Coculture Techniques; D004271:DNA, Fungal / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005656:Fungal Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005800:Genes, Fungal; D019337:Hematologic Neoplasms / Q000150:complications; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D009154:Mutation; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D019737:Transplants / Q000009:adverse effects
C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D000946:Antigens, Fungal; D052179:Antigens, Plant; D004271:DNA, Fungal; D005656:Fungal Proteins; D011994:Recombinant Proteins
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