Serum free light chain immunoassay as an adjunct to serum protein electrophoresis and immunofixation electrophoresis in the detection of multiple myeloma and other B-cell malignancies.
BACKGROUND
Protein and immunofixation electrophoresis of serum and urine are established as diagnostic aids for identifying monoclonal gammopathies. However, many patient sera sent to laboratories are not accompanied by urine samples and recent reports suggest the use of serum free light chain (sFLC) analysis in combination with serum protein electrophoresis (SPE) and immunofixation electrophoresis (lFE) could eliminate the need for urinalysis. The aim of the study was to assess the utility of sFLC measurement in addition to serum protein electrophoresis in the identification of patients with B-cell malignancies.


METHODS
A total of 952 serum samples were analysed by serum protein electrophoresis and those with abnormal bands were analysed by immunofixation. sFLCs were measured in a retrospective manner by automated assay.


RESULTS
In our study of 952 patient sera, it was found that FLC analysis identified 23 additional cases of B-cell malignancies which were missed by SPE.


CONCLUSIONS
The additional malignancies identified by sFLC analysis add support for its inclusion in the routine screening protocol for B-cell malignancies.
Harding
Stephen J
SJ
The Binding Site Ltd., Birmingham, UK. stephen.harding@bindingsite.co.uk
Mead
Graham P
GP
Bradwell
Arthur R
AR
Berard
Annie M
AM
D001797:Blood Protein Electrophoresis; D019937:Diagnostic Techniques and Procedures; D006801:Humans; D007118:Immunoassay; D007122:Immunoelectrophoresis; D007147:Immunoglobulin Light Chains / Q000097:blood*; D016393:Lymphoma, B-Cell / Q000097:blood / Q000175:diagnosis*; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000175:diagnosis*; D015203:Reproducibility of Results
D007147:Immunoglobulin Light Chains