title: Lack of Rb2/p130 genetic alteration in Malaysian nasopharyngeal carcinoma.
abstract: The retinoblastoma-related gene Rb2/p130 has been reported to be mutated in several malignancies such as lung cancer and Burkitt's lymphoma. Nasopharyngeal carcinoma (NPC) is a common cancer in Malaysia especially amongst the ethnic Chinese. We screened for Rb2/p130 gene (exons 19 to 21) mutations in 53 archival NPC samples via PCR-SSCP-direct sequencing approach. Only one sample had a base change which involved a serine to glycine substitution at codon 995 (S995G). We conclude that Rb2/p130 genetic alterations are infrequent in NPC and may not be essential for the pathogenesis of the disease.
authors.affiliation: Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. susanhoe@imr.gov.my
authors.forename: Susan Ling Ling
authors.initials: SL
authors.lastname: Hoe
authors.forename: Ee Soo
authors.initials: ES
authors.lastname: Lee
authors.forename: Alan Soo Beng
authors.initials: AS
authors.lastname: Khoo
authors.forename: Suat-Cheng
authors.initials: SC
authors.lastname: Peh
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D050717:Retinoblastoma-Like Protein p130 / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D050717:Retinoblastoma-Like Protein p130