Fourier transform-second-harmonic generation imaging of biological tissues.
Fourier transform-second-harmonic generation imaging is employed to obtain quantitative metrics of collagen fibers in biological tissues. In particular, the preferred orientation and maximum spatial frequency of collagen fibers for selected regions of interest in porcine trachea, ear, and cornea are determined. These metrics remain consistent when applied to collagen fibers in the ear, which can be expected from observation. Collagen fibers in the trachea are more random with large standard deviations in orientation, and large variations in maximum spatial frequency. In addition, these metrics are used to investigate structural changes through a 3D stack of the cornea. This technique can be used as a quantitative marker to assess the structure of collagen fibers that may change due to damage from disease or physical injury.
Rao
Raghu Ambekar Ramachandra
RA
Photonics Research of Bio/nano Environments (PROBE), Department of Electrical and Computer Engineering, University of Illinois Urbana-Champaign, 1406 W Green St, Urbana, IL 61801, USA.
Mehta
Monal R
MR
Toussaint
Kimani C
KC
D000818:Animals; D003094:Collagen / Q000737:chemistry*; D003315:Cornea / Q000473:pathology*; D004423:Ear / Q000473:pathology*; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008962:Models, Theoretical; D055095:Optics and Photonics; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013552:Swine; D014132:Trachea / Q000473:pathology*
D003094:Collagen