Cell encapsules with tunable transport and mechanical properties.
We utilized a microfluidic device with hydrodynamic flow focusing geometry to produce uniform agarose droplets in the range of 50 to 110 mum. The transport property of the thermally gelled particles was tailored by layer-by-layer (LBL) polyelectrolytes coating on the surface and was measured via the release rates of Rhodamine B. The mechanical strength of the capsules was further enhanced by a coating of silica nano-particles in addition to polyelectrolyte coatings. We demonstrated that yeast cells can be successfully encapsulated into agarose capsules.
Luo
Dawei
D
Artie McFerrin, Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122, USA and INEST (Interdisciplinary Network of Emerging Science and Technologies) Group Postgraduate Program, Philip Morris USA, Richmond, Virginia 23234, USA.
Pullela
Srinivasa Rao
SR
Marquez
Manuel
M
Cheng
Zhengdong
Z
J Control Release. 2000 May 15;66(2-3):281-92
10742587
Hum Gene Ther. 1993 Aug;4(4):433-40
8399490
Int J Pharm. 1999 Sep 30;187(1):115-23
10502618
Science. 1980 Nov 21;210(4472):908-10
6776628
Biomaterials. 2005 Jun;26(16):3327-31
15603828
Science. 2002 Aug 16;297(5584):1197-8
12183634
Trends Biotechnol. 1996 May;14(5):158-62
8645450
Transplant Proc. 1992 Jun;24(3):997
1604705
Biomicrofluidics. 2007 Jan 25;1(1):14105
19693354
Biomaterials. 2005 Aug;26(23):4786-92
15763258
Biotechnol Bioeng. 2004 Apr 20;86(2):168-73
15052636
Transplant Proc. 1992 Jun;24(3):952
1604680
Transplant Proc. 1992 Dec;24(6):2922-3
1466001
FASEB J. 2002 Feb;16(2):213-5
11772948
Nat Med. 2003 Jan;9(1):104-7
12514721
Angew Chem Int Ed Engl. 1998 Mar 16;37(5):550-575
29711088
J Microencapsul. 1987 Jul-Sep;4(3):239-42
3332901
Science. 1964 Oct 23;146(3643):524-5
14190240
Hum Gene Ther. 1996 Jan;7(1):61-70
8825869
Electrophoresis. 2005 Oct;26(19):3716-24
16196106