Structure analysis of water-soluble polysaccharide CPPS3 isolated from Codonopsis pilosula.
Codonopsis pilosula is a perennial species of flowering plants propagated in Northeast Asia. A water-soluble polysaccharide, CPPS(3), was extracted from the root of Codonopsis pilosula by boiled water extraction and ethanol precipitation. The molecular weight was estimated to be 7.4 x 10(4) Da determined by using Gel permeation chromatography. Monosaccharide composition and the structure of the polysaccharide were determined by gas spectroscopy, Fourier transform IR (FT-IR) spectroscopy, NMR spectroscopy and mass spectroscopy and some chemical method analysis was made. The components were galactose, arabinose and rhamnose in the molar ratio of 1.13:1.12:1. The main chain of CPPS(3) is illustrated to be (1-->3)-linked-beta-GalpNAc, (1-->3)-linked-alpha-Rhap and (1-->2,3)-beta-Galp.
Zhang
Ya-jun
YJ
College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou, China. yajunzhang2005@yahoo.com.cn
Zhang
Li-xia
LX
Yang
Jing-feng
JF
Liang
Zhong-yan
ZY
D002850:Chromatography, Gel; D029745:Codonopsis / Q000737:chemistry*; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility; D013057:Spectrum Analysis / Q000379:methods; D014867:Water
C551158:CPPS3 polysaccharide, Codonopsis pilosula; D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water