The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass.
Lignocellulosic biomass is a renewable and abundant resource with great potential for bioconversion to value-added bioproducts. However, the biorefining process remains economically unfeasible due to a lack of biocatalysts that can overcome costly hurdles such as cooling from high temperature, pumping of oxygen/stirring, and, neutralization from acidic or basic pH. The extreme environmental resistance of bacteria permits screening and isolation of novel cellulases to help overcome these challenges. Rapid, efficient cellulase screening techniques, using cellulase assays and metagenomic libraries, are a must. Rare cellulases with activities on soluble and crystalline cellulose have been isolated from strains of Paenibacillus and Bacillus and shown to have high thermostability and/or activity over a wide pH spectrum. While novel cellulases from strains like Cellulomonas flavigena and Terendinibacter turnerae, produce multifunctional cellulases with broader substrate utilization. These enzymes offer a framework for enhancement of cellulases including: specific activity, thermalstability, or end-product inhibition. In addition, anaerobic bacteria like the clostridia offer potential due to species capable of producing compound multienzyme complexes called cellulosomes. Cellulosomes provide synergy and close proximity of enzymes to substrate, increasing activity towards crystalline cellulose. This has lead to the construction of designer cellulosomes enhanced for specific substrate activity. Furthermore, cellulosome-producing Clostridium thermocellum and its ability to ferment sugars to ethanol; its amenability to co-culture and, recent advances in genetic engineering, offer a promising future in biofuels. The exploitation of bacteria in the search for improved enzymes or strategies provides a means to upgrade feasibility for lignocellulosic biomass conversion, ultimately providing means to a 'greener' technology.
Maki
Miranda
M
Biorefining Research Initiative, Lakehead University, Thunder Bay, Ontario, Canada.
Leung
Kam Tin
KT
Qin
Wensheng
W
D001407:Bacillus / Q000201:enzymology / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology* / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism*; D044603:Cellulosomes / Q000378:metabolism; D048013:Acetivibrio thermocellus / Q000201:enzymology / Q000378:metabolism
D002480:Cellulase
Biochemistry. 1996 Nov 5;35(44):13895-906
8909286
Plasmid. 2003 Jan;49(1):2-8
12583995
Bioresour Technol. 2005 Dec;96(18):1959-66
16112483
J Bacteriol. 2004 Oct;186(19):6544-52
15375136
Appl Environ Microbiol. 1984 May;47(5):1126-9
16346540
J Microbiol Biotechnol. 2007 Nov;17(11):1782-8
18092461
Anal Bioanal Chem. 2004 Apr;378(7):1710-5
14634707
Structure. 1995 Sep 15;3(9):853-9
8535779
Cell Res. 2007 Nov;17(11):963-5
17971808
Appl Environ Microbiol. 2004 Sep;70(9):5704-7
15345466
Appl Environ Microbiol. 1981 Apr;41(4):1060-2
16345748
J Bacteriol. 1980 Nov;144(2):569-78
7430065
Appl Environ Microbiol. 2000 Feb;66(2):788-93
10653752
Curr Microbiol. 2008 Nov;57(5):503-7
18810533
Science. 1994 Jul 22;265(5171):524-8
8036495
Microb Ecol. 2008 Oct;56(3):403-11
18196313
Appl Environ Microbiol. 2005 Nov;71(11):7099-106
16269746
J Bacteriol. 1997 Nov;179(21):6595-601
9352905
Science. 1990 Jul 27;249(4967):380-6
2377893
Biotechnol Lett. 2009 May;31(5):751-7
19169889
J Gen Microbiol. 1977 Jan;98(1):109-15
401863
Biotechnol Lett. 2004 Sep;26(17):1329-33
15604759
FEBS Lett. 1998 Mar 27;425(2):352-4
9559678
J Biotechnol. 2006 Sep 18;125(3):338-50
16644050
Appl Environ Microbiol. 2004 Feb;70(2):883-90
14766568
Bioresour Technol. 2007 Oct;98(14):2727-33
17123815
J Bacteriol. 1998 Apr;180(7):1709-14
9537366
FEMS Microbiol Lett. 2008 Oct;287(2):205-11
18752623
Antonie Van Leeuwenhoek. 2006 Feb;89(2):211-9
16710633
Appl Environ Microbiol. 2007 Jun;73(12):3822-32
17468286
J Ind Microbiol Biotechnol. 2001 Nov;27(5):275-80
11781801
Appl Environ Microbiol. 1997 Dec;63(12):4721-8
9406391
J Mol Biol. 1998 Jan 16;275(2):309-25
9466911
J Biol Chem. 2005 Apr 22;280(16):16325-34
15705576
Annu Rev Microbiol. 2004;58:521-54
15487947
J Appl Microbiol. 2005;99(3):509-17
16108792
J Microbiol Methods. 2007 Sep;70(3):452-64
17658189
Nature. 2001 Jan 11;409(6817):253-7
11196654
Ann N Y Acad Sci. 2008 Mar;1125:267-79
18096849
Appl Microbiol Biotechnol. 1996 Mar;45(1-2):127-31
11536742
J Biol Chem. 2000 May 5;275(18):13708-12
10788490
Wei Sheng Wu Xue Bao. 2006 Oct;46(5):783-8
17172029
Mol Microbiol. 2002 Aug;45(3):617-26
12139610
Appl Biochem Biotechnol. 2004 Spring;113-116:287-97
15054213
Appl Environ Microbiol. 1990 Jan;56(1):37-42
16348106
J Microbiol Methods. 2009 Mar;76(3):295-300
19150471
Appl Microbiol Biotechnol. 2005 Jul;68(1):46-52
15666145
Proteins. 2008 Mar;70(4):1280-93
17876824
Trends Microbiol. 1999 Jul;7(7):275-81
10390637
Appl Biochem Biotechnol. 2005 Spring;121-124:129-48
15917594
Chem Rec. 2008;8(6):364-77
19107866
Appl Biochem Biotechnol. 2002 Spring;98-100:383-94
12018266
Protein Eng. 1994 Nov;7(11):1379-86
7700870
Parasitology. 2004 Oct;129(Pt 4):465-72
15521635
Appl Environ Microbiol. 1992 Jan;58(1):421-5
1311546
Appl Environ Microbiol. 2000 Nov;66(11):4817-21
11055929
Appl Environ Microbiol. 1981 Jun;41(6):1337-43
16345787
FEMS Microbiol Lett. 2004 Nov 15;240(2):237-43
15522513
J Biol Chem. 1985 Jun 25;260(12):7442-51
3997879
Appl Environ Microbiol. 2005 Dec;71(12):8069-76
16332787
Biotechnol Bioeng. 1993 Apr 15;41(9):846-53
18609632
J Biotechnol. 2008 Jul 31;135(4):351-7
18582975
Biochemistry. 1991 Sep 3;30(35):8530-4
1909565
Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11206-10
16837571
Gene. 2001 Jun 13;271(1):13-20
11410361
Appl Environ Microbiol. 2007 Dec;73(23):7785-8
17933945
J Biol Chem. 2004 Oct 8;279(41):42787-93
15252054
Biotechnol Lett. 2004 Feb;26(4):281-5
15055762
Trends Biotechnol. 2008 Sep;26(9):483-9
18675483
FEMS Microbiol Lett. 2006 Aug;261(2):224-30
16907724
Microbiol Mol Biol Rev. 2005 Mar;69(1):124-54
15755956
Biomed Chromatogr. 1999 Dec;13(8):516-9
10611604
J Biol Chem. 1995 Dec 8;270(49):29314-22
7493964
J Biol Chem. 2004 Mar 19;279(12):11495-502
14660638
Lett Appl Microbiol. 2008 Jul;47(1):46-53
18554262
Biochem Biophys Res Commun. 2002 Feb 22;291(2):425-30
11846423
Appl Microbiol Biotechnol. 2008 Jun;79(4):579-87
18415095
Biochemistry. 2000 May 2;39(17):4963-70
10819960
Appl Microbiol Biotechnol. 2001 Sep;56(5-6):634-49
11601609
Cytometry. 1988 Jul;9(4):394-404
3135986
J Biol Chem. 2002 Dec 20;277(51):49621-30
12397074
J Biol Chem. 2000 Sep 15;275(37):28843-8
10871610
Biotechnol Lett. 2008 Jun;30(6):1061-8
18224282
J Appl Microbiol. 2009 Jul;107(1):245-56
19302301
Curr Microbiol. 2008 Jul;57(1):39-44
18379842
Nat Struct Biol. 1997 Oct;4(10):810-8
9334746
J Ind Microbiol Biotechnol. 2009 Jul;36(7):961-70
19390881
Biotechnol Adv. 2006 Sep-Oct;24(5):452-81
16690241
J Biol Chem. 1997 Sep 19;272(38):24016-23
9295354
Curr Opin Biotechnol. 2005 Oct;16(5):577-83
16154338
Mol Biotechnol. 2008 Oct;40(2):195-201
18576142
J Ind Microbiol Biotechnol. 2009 Apr;36(4):585-98
19189143
Science. 1989 Feb 10;243(4892):771-81
17820424