Role of macromolecular depletion in red blood cell adhesion.
The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference reflection microscopy. Our experimental results indicate that adhesion can be induced in the presence of dextran with a molecular mass >or=70 kDa and that the induced forces are strong enough to significantly suppress membrane undulations. The overall dependence of the adhesion energies on the polymer concentration is consistent with the assumption that macromolecular depletion induces this attractive interaction. In conclusion, our results indicate that depletion interaction might play a significant role in RBC adhesion via initiating close contacts, and thus suggest the importance of depletion forces for RBC interactions and its relevance to a wide variety of in vitro and in vivo cell-cell and cell-surface interactions.
Zhang
Zhengwen W
ZW
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Neu
Björn
B
D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D003198:Computer Simulation; D004903:Erythrocyte Aggregation / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D046911:Macromolecular Substances / Q000494:pharmacology*; D008955:Models, Cardiovascular
D046911:Macromolecular Substances
Electrophoresis. 2002 Jul;23(13):2103-9
12210265
Biorheology. 1996 Jul-Oct;33(4-5):333-51
8977659
Blood. 2002 Mar 1;99(5):1564-71
11861269
Microvasc Res. 1993 Jan;45(1):33-45
8479340
J Cell Sci. 1980 Feb;41:135-49
7364878
Biophys J. 1973 Mar;13(3):245-64
4697236
Electrophoresis. 2009 Feb;30(3):450-6
19145596
J Cell Sci. 1985 Apr;75:279-301
3900106
Biophys J. 1979 Jun;26(3):507-26
262429
Clin Hemorheol Microcirc. 2004;30(3-4):181-4
15258342
Biophys J. 1983 Jul;43(1):27-30
6882860
Biophys J. 1997 Jul;73(1):245-57
9199789
Biorheology. 2001;38(1):39-51
11381164
Biophys J. 2002 Nov;83(5):2482-90
12414682
Biochim Biophys Acta. 2006 Dec;1760(12):1772-9
17049748
Biophys J. 2003 Jul;85(1):75-84
12829466
Biophys J. 1974 Dec;14(12):923-31
4429770
Biophys J. 2008 Sep 15;95(6):3059-65
18586848