SOSS complexes participate in the maintenance of genomic stability.
Proteins that bind to single-stranded DNA (ssDNA) are essential for DNA replication, recombinational repair, and maintenance of genomic stability. Here, we describe the characterization of an ssDNA-binding heterotrimeric complex, SOSS (sensor of ssDNA) in human, which consists of human SSB homologs hSSB1/2 (SOSS-B1/2) and INTS3 (SOSS-A) and a previously uncharacterized protein C9orf80 (SOSS-C). We have shown that SOSS-A serves as a central adaptor required not only for SOSS complex assembly and stability, but also for facilitating the accumulation of SOSS complex to DNA ends. Moreover, SOSS-depleted cells display increased ionizing radiation sensitivity, defective G2/M checkpoint, and impaired homologous recombination repair. Thus, our study defines a pathway involving the sensing of ssDNA by SOSS complex and suggests that this SOSS complex is likely involved in the maintenance of genome stability.
Huang
Jun
J
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06520, USA.
Gong
Zihua
Z
Ghosal
Gargi
G
Chen
Junjie
J
D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology; D002453:Cell Cycle / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004706:Endodeoxyribonucleases; D042822:Genomic Instability; D006801:Humans; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology; D055550:Protein Stability; D011995:Recombination, Genetic
D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; C543506:INIP protein, human; C543505:INTS3 protein, human; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; C504091:NABP2 protein, human; D004706:Endodeoxyribonucleases; C410741:RBBP8 protein, human
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