title: Comparative analysis of cell proliferation ratio in oral lichen planus, epithelial dysplasia and oral squamous cell carcinoma.
abstract: BACKGROUND
  Although oral lichen planus has been classified by the World Health Organization (WHO) as a potentially malignant disorder, such classification is still the target of much controversy.
  
  
  OBJECTIVE
  To evaluate the cell proliferation rate in oral lichen planus, comparing it to the rate observed in epithelial dysplasia and oral squamous cell carcinoma, aiming at indications which might indicate the potential for malignant transformation.
  
  
  METHODS
  Twenty-four cases of each lesion were submitted to the streptoavidin-biotin and AgNOR technique to evaluate the immunohistochemical expression of PCNA and the mean NORs/nucleus, respectively.
  
  
  RESULTS
  Positivity for PCNA was observed in 58.33% of oral lichen planus cases, 83.33% of epithelial dysplasia cases and 91.67% of oral squamous cell carcinoma cases. Chi-squared test showed that the number of positive cases for PCNA was significantly lower in oral lichen planus than in oral squamous cell carcinoma (p<0.05). No significant statistical difference between oral lichen planus and epithelial dysplasia (p>0.05) and between the epithelial dysplasia and oral squamous cell carcinoma (p>0.05) was observed. The mean NORs/nucleus in oral lichen planus, epithelial dysplasia and oral squamous cell carcinoma were 1.74+/-0.32, 2.42+/-0.62 e 2.41+/-0.61, respectively. Variance analysis (ANOVA) revealed significant statistical difference between oral lichen planus and the other studied lesions (p<0.05).
  
  
  CONCLUSIONS
  Oral lichen planus cell proliferation rate was less than in oral epithelial dysplasia and oral squamous cell carcinoma which might explain the lower malignant transformation rate.
authors.affiliation: Department of Bioscience and Oral Diagnosis, São José dos Campos Dental School, São Paulo State University, São José dos Campos, SP, Brazil. facgs@uol.com.br
authors.forename: Fernando-Augusto-Cervantes-Garcia
authors.initials: FA
authors.lastname: de Sousa
authors.forename: Thaís-Cachuté
authors.initials: TC
authors.lastname: Paradella
authors.forename: Yasmin-Rodarte
authors.initials: YR
authors.lastname: Carvalho
authors.forename: Luiz-Eduardo-Blumer
authors.initials: LE
authors.lastname: Rosa
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D049109:Cell Proliferation; D006801:Humans; D017676:Lichen Planus, Oral / Q000473:pathology*; D009061:Mouth Mucosa / Q000473:pathology*; D009062:Mouth Neoplasms / Q000473:pathology*