Haptoglobin polymorphism in Mongolian population: comparison of the two genotyping methods.
BACKGROUND
Haptoglobin (Hp) polymorphisms have been suggested to be associated with many pathological conditions including cardiovascular diseases, infectious diseases, and type 2 diabetes.


METHODS
We examined the association between HP genotypes and type 2 diabetes or anthropometric and clinical features in 946 Mongolians. HP genotypes were determined by two methods, TaqMan-based real-time PCR and conventional PCR. Hp phenotyping was also performed by polyacrylamide gel electrophoresis and peroxidase staining in a few selected samples.


RESULTS
The 2 HP genotyping methods showed consistent results in 943 of 946 samples. Three samples that showed different results in the 2 PCR methods were determined to be heterozygous for the Hp Johnson allele by phenotyping. We did not find any association between HP genotypes and type 2 diabetes or the anthropometric and clinical features examined in the population studied.


CONCLUSIONS
TaqMan-based HP genotyping is reliable and will be useful for high-throughput association studies. Further studies of large numbers of subjects and functional differences in Hp types are needed.
Nakamura
Hitomi
H
Department of Forensic Medicine and Human Genetics, Kurume University School of Medicine, Kurume, Fukuoka, Japan.
Soejima
Mikiko
M
Munkhtulga
Lkhagvasuren
L
Iwamoto
Sadahiko
S
Koda
Yoshiro
Y
D044466:Asian People / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D005260:Female; D005838:Genotype; D006242:Haptoglobins / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D008986:Mongolia; D011110:Polymorphism, Genetic
D006242:Haptoglobins