Chlamydia trachomatis alters iron-regulatory protein-1 binding capacity and modulates cellular iron homeostasis in HeLa-229 cells.
Chlamydia trachomatis (CT) is the leading cause of diseases related to reproductive health and iron plays important role in chlamydial pathogenesis. Iron homeostasis in chlamydia-infected cells is not clear thus far. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in CT-infected HeLa-229 cells. Expression of iron-regulatory protein (IRP)-1 predominates over IRP-2 in infected cells. In infected cells, attenuated binding activity of IRP-iron responsive elements (IREs) is observed using the electrophoretic mobility-shift assay. These results suggest that iron homeostasis is modulated in CT-infected HeLa cells at the interface of acquisition and commensal use of iron.
Vardhan
Harsh
H
Division of Microbiology/Tissue Culture, Institute of Pathology, Indian Council of Medical Research (ICMR), Safdarjung Hospital Campus, New Delhi, Pin 110029, India.
Bhengraj
Apurb R
AR
Jha
Rajneesh
R
Singh Mittal
Aruna
A
D000704:Analysis of Variance; D001052:Apoferritins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002690:Chlamydia Infections / Q000235:genetics / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000378:metabolism*; D015536:Down-Regulation; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000378:metabolism*; D035941:Iron Regulatory Protein 1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D035942:Iron Regulatory Protein 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011990:Receptors, Transferrin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020218:Response Elements; D015854:Up-Regulation
D011990:Receptors, Transferrin; D001052:Apoferritins; D007501:Iron; D035941:Iron Regulatory Protein 1; D035942:Iron Regulatory Protein 2
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