title: It's all in the details: methods in breast development and cancer.
abstract: The inaugural European Network for Breast Development and Cancer (ENBDC) meeting on 'Methods in Mammary Gland Development and Cancer' was held in Weggis, Switzerland last April. The goal was to discuss the details of techniques used to study mammary gland biology and tumourigenesis. Highlights of this meeting included the use of four-colour fluorescence for protein co-localisation in tissue microarrays, genome analysis at single cell resolution, technical issues in the isolation of normal and tumour stem cells, and the use of mouse models and mammary gland transplantations to elucidate gene function in mammary development and to study drug resistance in breast cancer.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research (FMI), Maulbeerstr, 66; CH-4058 Basel, Switzerland. Bentires@fmi.ch
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bentires-Alj
authors.forename: Robert B
authors.initials: RB
authors.lastname: Clarke
authors.forename: Jos
authors.initials: J
authors.lastname: Jonkers
authors.forename: Matthew
authors.initials: M
authors.lastname: Smalley
authors.forename: Torsten
authors.initials: T
authors.lastname: Stein
mesh_terms: D000818:Animals; D001940:Breast / Q000166:cytology / Q000254:growth & development*; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D019008:Drug Resistance, Neoplasm; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D005813:Genes, Synthetic; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D053569:Keratin-15; D053555:Keratin-5 / Q000235:genetics; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology; D008495:Medical Oncology / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010338:Pathology, Clinical / Q000379:methods*; D011401:Promoter Regions, Genetic; D013234:Stem Cells / Q000166:cytology
chemical_list: D053569:Keratin-15; D053555:Keratin-5; C492053:Krt15 protein, mouse
references.citation: Nature. 2008 Dec 4;456(7222):593-8
references.pmid: 19052619
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11958-63
references.pmid: 16877544
references.citation: Cell Stem Cell. 2009 May 8;4(5):427-39
references.pmid: 19427292
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12111-6
references.pmid: 17626182
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12117-22
references.pmid: 17626183
references.citation: Breast Cancer Res. 2006;8(1):R7
references.pmid: 16417656
references.citation: Nat Cell Biol. 2008 Jun;10(6):716-22
references.pmid: 18469806
references.citation: Cancer Res. 2009 Feb 15;69(4):1302-13
references.pmid: 19190339
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):17079-84
references.pmid: 18971340
references.citation: J Cell Biol. 2007 Jan 1;176(1):19-26
references.pmid: 17190790
references.citation: BMC Cell Biol. 2008 Mar 19;9:13
references.pmid: 18366689