Swiprosin-1 is expressed in mast cells and up-regulated through the protein kinase C beta I/eta pathway.
Swiprosin-1 exhibits the highest expression in CD8(+) T cells and immature B cells and has been thought to play a role in lymphocyte physiology. Here we report that swiprosin-1 is also expressed in mast cells and up-regulated in both in vitro cultured mast cells by phorbol ester and in vivo model tissues of passive cutaneous anaphylaxis and atopic dermatitis. Targeted inhibition of the specific protein kinase C (PKC) isotypes by siRNA revealed that PKC-beta I/eta are involved in the expression of swiprosin-1 in the human mast cell line HMC-1. In contrast, down-regulation of swiprosin-1 by A23187 or ionomycin suggests that calcium-signaling plays a negative role. The ectopic expression of swiprosin-1 augmented PMA/A23187-induced NF-kappaB promoter activity, and resulted in increased expression of cytokines. Moreover, knock-down of swiprosin-1 attenuated PMA/A23187-induced cytokine expression. Collectively, these results suggest that swiprosin-1 is a PKC-beta I/eta-inducible gene and it modulates mast cell activation through NF-kappaB-dependent pathway.
Thylur
Ramesh P
RP
Department of Life Science, Cell Dynamics Research Center, BioImaging Research Center, and Research Center for Biomolecular Nanotechnology, GIST, Gwangju 500-712, South Korea.
Kim
Young-Dae
YD
Kwon
Min-Sung
MS
Oh
Hyun-Mee
HM
Kwon
Ho-Keun
HK
Kim
Sang-Hyun
SH
Im
Sin-Hyeog
SH
Chun
Jang-Soo
JS
Park
Zee-Yong
ZY
Jun
Chang-Duk
CD
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D020013:Calcium Signaling / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D003872:Dermatitis / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; D016207:Cytokines; C502299:EFHD2 protein, human; C530752:EFHD2 protein, mouse; D007476:Ionophores; D016328:NF-kappa B; D047428:Protein Kinase Inhibitors; C081975:protein kinase C eta; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D013755:Tetradecanoylphorbol Acetate