Impact of type 1 diabetes on cardiac fibroblast activation: enhanced cell cycle progression and reduced myofibroblast content in diabetic myocardium.
Diabetic patients are prone to developing myocardial fibrosis and suffer from decreased wound healing capabilities. The purpose of this study was to determine whether diabetes alters cardiac fibroblast activity in the myocardium in a 6-wk streptozotocin-induced type 1 diabetic model. In vivo echocardiography indicated significant dilation of the left ventricle (LV) in the diabetic animals, while cardiac function was comparable to that in the normal group. We isolated cardiac fibroblasts from diabetic and control hearts and observed increased proliferation of the diabetic fibroblasts. Microarray analysis using mRNA collected from whole LVs revealed downregulation of known inhibitors of proliferation, p53 and p21, in the diabetic group, consistent with our proliferation data. Western blot analysis confirmed a reduction in p53 protein expression in the diabetic hearts compared with control. We explored the potential signaling underlying the downregulation of these cell cycle mediators and determined that activated Akt, a signal that inhibits p53, was elevated in the diabetic group. Surprisingly, the hearts from the diabetic group contained lower levels of the myofibroblast marker α-smooth muscle actin (α-SMA) and higher levels of desmin and platelet endothelial cell adhesion molecule (PECAM). The isolated fibroblasts from the diabetic group also contained significantly less α-SMA. These data suggest that early-stage diabetic hearts contain highly proliferative fibroblasts, which predisposes the diabetic myocardium to fibrosis, but have fewer myofibroblasts, which may compromise wound healing.
Shamhart
Patricia E
PE
Northeastern Ohio Universities College of Medicine, Dept. of Integrative Biomedical Sciences, 4209 State Route 44, Rootstown, OH 44272-0095, USA. jgmeszar@neoucom.edu
Luther
Daniel J
DJ
Hodson
Ben R
BR
Koshy
John C
JC
Ohanyan
Vahagn
V
Meszaros
J Gary
JG
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000000981:diagnostic imaging / Q000473:pathology*; D004452:Echocardiography; D005347:Fibroblasts / Q000502:physiology*; D008297:Male; D046228:Microarray Analysis; D009206:Myocardium / Q000166:cytology / Q000473:pathology*; D058628:Myofibroblasts / Q000502:physiology*; D010641:Phenotype; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology
D001786:Blood Glucose; D018797:Cell Cycle Proteins; D012313:RNA