title: Decomposition of alpha-Tocopheryl Glycosides in Rat Tissues.
abstract: BACKGROUND: The aim of our investigation was to estimate the stability of alpha-tocopheryl O-glycosides in relation to activity of exoglycosidases in selected rat tissues. MATERIAL AND METHODS: Acetylated glycosides were obtained in glucosidation of alpha-tocopherol using the Helferich method. The activity of exoglycosidases was determined by the Zwierz et al. method. Protein concentrations were determined by the biuret method. The concentration of released alpha-tocopherol was determined with the HPLC method. RESULTS: The comparison of the amount of released alpha-tocopherol with the amount of released p-nitrophenol shows that glycoside bound in 2a-5a derivatives of alpha-tocopherol undergoes hydrolysis significantly harder than in appropriate 2b-5bp-nitrophenyl derivatives. CONCLUSION: The results indicate that tocopheryl O-glycosides are more resistant to enzymatic hydrolysis than appropriate p-nitrophenol O-glycosides 2a-5a. Among examined tocopheryl O-glycosides, galactoside 4 is the only compound that caused the significant increase in tocopherol concentration, as compared to its endogenic content.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Knaś
authors.forename: Piotr
authors.initials: P
authors.lastname: Wałejko
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Maj
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Hryniewicka
authors.forename: Stanisław
authors.initials: S
authors.lastname: Witkowski
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Borzym-Kluczyk
authors.forename: Danuta
authors.initials: D
authors.lastname: Dudzik
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zwierz
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