title: Differential suppressive effect of promyelocytic leukemia protein on the replication of different subtypes/strains of influenza A virus.
abstract: Promyelocytic leukemia protein (PML) plays an important role in the defense against a number of viruses, including influenza A virus. However, the sensitivity of influenza A virus subtypes/strains to PML is unknown. We investigated the role of PML in the replication of different influenza A virus subtypes/strains using pan-PML knock-down A549 cells and PML-VI-overexpressed MDCK cells. We found that (i) depletion of pan-PML by siRNA rendered A549 cells more susceptible to influenza A virus strains PR8(H1N1) and ST364(H3N2), but not to strains ST1233(H1N1), Qa199(H9N2) and Ph2246(H9N2); (ii) overexpression of PML-VI in MDCK cells conferred potent resistance to PR8(H1N1) infection, while lacked inhibitory activity to ST1233(H1N1), ST364(H3N2), Qa199(H9N2) and Ph2246(H9N2). Our results suggest that the antiviral effect of PML on influenza A viruses is viral subtype/strain specific.
authors.affiliation: Department of Viral Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, PR China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Li
authors.forename: Gefei
authors.initials: G
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Dangui
authors.initials: D
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Chen
authors.forename: Yanxuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Kangsheng
authors.initials: K
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004285:Dogs; D055785:Gene Knockdown Techniques; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D053122:Influenza A Virus, H3N2 Subtype / Q000502:physiology*; D053127:Influenza A Virus, H9N2 Subtype / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human