title: Complementary DNA sequences encoding the multimammate rat MHC class II DQ alpha and beta chains and cross-species sequence comparison in rodents.
abstract: Sequences of the complete open reading frame (ORF) for rodents major histocompatibility complex (MHC) class II genes are rare. Multimammate rat (Mastomys natalensis) complementary DNA (cDNA) encoding the alpha and beta chains of MHC class II DQ gene was cloned from a rapid amplifications of cDNA Emds (RACE) cDNA library. The ORFs consist of 801 and 771 bp encoding 266 and 256 amino acid residues for DQB and DQA, respectively. The genomic structure of Mana-DQ genes is globally analogous to that described for other rodents except for the insertion of a serine residue in the signal peptide of Mana-DQB, which is unique among known rodents.
authors.affiliation: Department of Biology, University of Antwerp, Groenenborgerlaan 171, Antwerp, Belgium. joelle.gouydebellocq@ua.ac.be
authors.forename: J Goüy
authors.initials: JG
authors.lastname: de Bellocq
authors.forename: H
authors.initials: H
authors.lastname: Leirs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000737:chemistry / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D021382:Protein Sorting Signals / Q000235:genetics; D051381:Rats; D012377:Rodentia / Q000235:genetics* / Q000276:immunology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000235:genetics; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C057615:HLA-DQbeta antigen; D000949:Histocompatibility Antigens Class II; D021382:Protein Sorting Signals; D012694:Serine