New reusable Celite/ethylene glycol cartridges for selective chromatography of steroids before immunoassay.
OBJECTIVE
Preparation of reusable and easy to handle Celite chromatographic columns.


METHODS
Weighting precise Celite quantities in cartridges and introducing ethylene glycol methanol solutions. The chromatographic solvents pass throughout Celite under negative pressure.


RESULTS
These new minicolumns are reusable. The steroid recoveries' coefficients of variation are less than 10%, and the steroid separation is good.


CONCLUSIONS
The reusable Celite cartridge use before steroid immunoassays is easier and less time-consuming than classical glass Celite minicolumns.
Giton
Frank
F
AP-HP CIB GHU Sud Henri Mondor, Faculté de Médecine, 8 rue du Général Sarrail, Créteil, France. giton@univ-paris12.fr
Guéchot
Jérôme
J
Fiet
Jean
J
D002845:Chromatography / Q000379:methods*; D019855:Ethylene Glycol / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D015203:Reproducibility of Results
D019855:Ethylene Glycol