title: IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts.
abstract: By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age- and gender-matched healthy controls with normal human skin fibroblasts and HEp-2 cells antigens. Serum IgG reactivities of patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies differed significantly from those of healthy controls or systemic sclerosis patients in other groups for reactivity with fibroblast proteins. IgG from patients with antitopoisomerase 1 antibodies bound to a 90 kDa fibroblast band and to a 100 kDa protein band in a HEp-2 cell protein extract. These two bands were further identified as DNA topoisomerase 1. Our results indicate that IgG from patients with diffuse systemic sclerosis bind DNA topoisomerase 1 in normal human fibroblasts extracts.
authors.affiliation: Paris-Descartes University, Faculty of Medicine, UPRES-EA 4058, Paris, France;
authors.forename: Mathieu C
authors.initials: MC
authors.lastname: Tamby
authors.forename: Amélie
authors.initials: A
authors.lastname: Servettaz
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tamas
authors.forename: Joseph
authors.initials: J
authors.lastname: Reinbolt
authors.forename: Frédéric
authors.initials: F
authors.lastname: Caux
authors.forename: Olivier
authors.initials: O
authors.lastname: Meyer
authors.forename: Yannick
authors.initials: Y
authors.lastname: Allanore
authors.forename: André
authors.initials: A
authors.lastname: Kahan
authors.forename: Loïc
authors.initials: L
authors.lastname: Guillevin
authors.forename: Luc
authors.initials: L
authors.lastname: Mouthon
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