title: Mapping the interaction site of prion protein and Sho.
abstract: The cellular prion protein (PrP(C)) is a highly conserved protein among mammals and is considered to have important cellular functions. Despite decades of intensive research, however, the physiological function of PrP(C) remains unclear. Sho (Shadoo, shadow of prion protein) and PrP(C) have similar N-terminals, which suggests that the two proteins share biological functions. Using truncation mutants of both proteins and yeast two-hybrid analysis, with validation by co-immunoprecipitation and surface plasmon resonance (SPR), we have identified an interaction between Sho 61-77 and PrP(C) 108-126 domains. This indicates that Sho may play a role in the physiological function of PrP(C) and prion pathogenesis.
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun, 130062 China.
authors.forename: Wan
authors.initials: W
authors.lastname: Jiayu
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.forename: Xu
authors.initials: X
authors.lastname: Ming
authors.forename: Wang
authors.initials: W
authors.lastname: Xiong
authors.forename: Wu
authors.initials: W
authors.lastname: Songbo
authors.forename: Song
authors.initials: S
authors.lastname: Bocui
authors.forename: Liu
authors.initials: L
authors.lastname: Wensen
authors.forename: Li
authors.initials: L
authors.lastname: Jiping
authors.forename: Meng
authors.initials: M
authors.lastname: Keying
authors.forename: Li
authors.initials: L
authors.lastname: Zhongyi
authors.forename: Gao
authors.initials: G
authors.lastname: Hongwei
mesh_terms: D000818:Animals; D058851:GPI-Linked Proteins; D047468:Immunoprecipitation; D051379:Mice; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D011328:Prions / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance; D020798:Two-Hybrid System Techniques
chemical_list: D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; D011328:Prions; C523856:Sprn protein, mouse
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