Immunostimulatory activities of a low molecular weight antitumoral polysaccharide isolated from Agaricus blazei Murill (LMPAB) in Sarcoma 180 ascitic tumor-bearing mice.
LMPAB is a linear beta-(1-3)-glucan we isolated from polysaccharide extract of Agaricus blazei Murill (AbM). Effects of LMPAB on splenic natural killer (NK) cell activity, splenocyte proliferation, index of spleen and thymus, IFN-gamma expression in spleen and the concentration of IL-12, IL-18 and TNF-alpha in serum of S180 ascitic tumor-bearing mice were detected. The results showed that intraperitoneal injection of LMPAB (100 mg x kg(-1) x d(-1)) significantly increased the thymus index. LMPAB augmented splenic NK cell activity in a dose-dependent manner (50-200 mg x kg(-1) x d(-1)). The concanavalin A (3 microg/ ml) stimulated splenocyte proliferation was significantly enhanced by LMPAB at dosages of 50, 100 or 200 mg x kg(-1) x d(-1). Further studies showed that LMPAB (50, 100 or 200 mg x kg(-1) x d(-1), 14d) significantly increased the production of IL-12, TNF-alpha, IL-18 and the expression IFN-gamma as determined by ELISA and immunohistochemistry, respectively. These results clearly indicate that the anti-tumor effects of LMPAB are closely associated with up-regulation of activity of NK cells, expression of IFN-gamma in spleen and the systemic level of IL-12, IL-18 and TNF-alpha in tumor-bearing mice.
Niu
Ying-Cai
YC
The Institute of Medicine, Qiqihar Medical College, 249 Heping Road, Fula'erji District, Qiqihar 161042, China.
Liu
Ji-Cheng
JC
Zhao
Xue-Mei
XM
Su
Fu-Qin
FQ
Cui
Hong-Xia
HX
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D018664:Interleukin-12 / Q000096:biosynthesis; D020382:Interleukin-18 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000276:Adjuvants, Immunologic; D004396:Coloring Agents; D016207:Cytokines; D007202:Indicators and Reagents; D020382:Interleukin-18; D011134:Polysaccharides; D013778:Tetrazolium Salts; D013844:Thiazoles; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma; C022616:thiazolyl blue