title: Low temperature acclimation mediated by ethanol production is essential for chilling tolerance in rice roots.
abstract: Rice seedlings (Oryza sativa L.) were subjected to low temperature pretreatment (LT-PT; 10 degrees C) for various length of time followed by a 48-h chilling temperature stress (2 degrees C). Chilling tolerance of rice roots was improved with increasing duration of LT-PT, but HT-PT longer than 12 h gave no additional improvement. LT-PT did not change in fatty acid composition in rice roots under the present experimental condition. Alcohol dehydrogenase (ADH) activity and ethanol concentration in the roots were increased with increasing duration of LT-PT up to 12 h, which indicates that LT-PT increased ethanol fermentation in the roots. 4-Methylpyrazole, a potent inhibitor of ADH, reduced the ethanol concentration and the chilling tolerance in the roots. This reduction of the chilling tolerance recovered with exogenously applied ethanol. Ethanol also induced 21- and 33-kD protein synthesis in the roots and these proteins may contribute the improvement of the tolerance. The present research suggests that LT-PT may increase chilling tolerance in rice roots owing to ethanol production, and ethanol may trigger a signal transduction cascade, which might lead to a decrease in membrane damage and injury.
authors.affiliation: Department of Applied Biological Science; Faculty of Agriculture; Kagawa University; Miki, Kagawa, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kato-Noguchi
references.citation: Plant Physiol. 1991 Mar;95(3):748-52
references.pmid: 16668049
references.citation: Plant Physiol. 1993 Mar;101(3):833-837
references.pmid: 12231733
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
references.pmid: 15012220
references.citation: Plant Physiol. 1994 Apr;104(4):1359-70
references.pmid: 8016266
references.citation: Science. 1990 May 4;248(4955):583-5
references.pmid: 2159183
references.citation: Plant Physiol Biochem. 2004 Dec;42(10):841-6
references.pmid: 15596104
references.citation: EMBO J. 1992 Jun;11(6):2357-64
references.pmid: 1600951
references.citation: J Photochem Photobiol B. 2000 Aug;57(1):60-5
references.pmid: 11100838
references.citation: Plant Physiol. 1994 Feb;104(2):479-496
references.pmid: 12232097
references.citation: Plant Physiol. 1989 Jul;90(3):1049-56
references.pmid: 16666850
references.citation: Trends Plant Sci. 1999 Aug;4(8):320-325
references.pmid: 10431222
references.citation: Plant Physiol. 1994 Apr;104(4):1131-1138
references.pmid: 12232153
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:223-250
references.pmid: 15012263
references.citation: Adv Microb Physiol. 1984;25:253-300
references.pmid: 6398622
references.citation: Physiol Plant. 2004 Apr;120(4):556-562
references.pmid: 15032817