High-yield hypocrellin A production in solid-state fermentation by Shiraia sp. SUPER-H168.
Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particle size 0.8-1 mm, initial moisture content 50%, and temperature 30 degrees C. Six kinds of external carbon source and seven kinds of external nitrogen source were evaluated, respectively, for HA production. Glucose and NaNO(3) were the best. The combination of them was optimized by the response surface method. The optimum compositions of the supplementary glucose and NaNO(3) were 1.65 g/100 g and 0.43 g/L, respectively. Hypocrellin A production reached 4.7 mg/g.
Cai
Yujie
Y
Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, 1800 Lihu road, Wuxi, Jiangsu, China. yu_jie_cai@yahoo.com.cn
Liang
Xiaohui
X
Liao
Xiangru
X
Ding
Yanrui
Y
Sun
Jun
J
Li
Xiaohui
X
D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism*; D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D018556:Crops, Agricultural / Q000382:microbiology; D004365:Drugs, Chinese Herbal; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D015394:Molecular Structure; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010316:Particle Size; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D019800:Phenol; D011809:Quinones / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D009566:Nitrates; D011809:Quinones; D019800:Phenol; D010569:Perylene; D002244:Carbon; C031618:sodium nitrate; D005947:Glucose; D009584:Nitrogen; C048388:hypocrellin A