title: Validation of derivative spectrophotometry method for determination of active ingredients from neuroleptics in pharmaceutical preparations.
abstract: First (DI) and second (D2) order derivative spectrophotometric method with an application of base line to peak technique was used for determination of active pharmaceutical ingredients (API) at two wavelengths: fluphenazine (D1 at lambda = 251 nm and lambda = 265 nm, D2 at lambda = 246 nm and lambda = 269 nm), pernazine (D1 at lambda = 246 nm and lambda = 258 nm, D2 at lambda = 254 nm and lambda = 262 nm), haloperidol (DI at = 235 nm and lambda = 253 nm, D2 at lambda = 230 nm and lambda = 246 nm), and promazine (D1 at lambda = 246 nm and lambda = 251 nm, D2 at lambda = 255 nm and lambda = 262 nm). Linear dependence of derivative values on analyte concentration is maintained in a range 3.12 microg x mL(-1) - 44.80 microg x mL(-1). Detection and determination limits are in the range 0.51 - 3.23 microg x mL(-1) and 1.27 microg x mL(-1) - 9.80 microg x mL(-1), respectively. Determination results of drug constituents are very accurate. Recovery percentage is in a range 95.50% - 103.60%.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Jagiellonian University, Collegium Medicum, Medyczna 9, 30-688 Kraków, Poland. mstolar@cm-uj.krakow.pl
authors.forename: Mariusz
authors.initials: M
authors.lastname: Stolarczyk
authors.forename: Anna
authors.initials: A
authors.lastname: Apola
authors.forename: Jan
authors.initials: J
authors.lastname: Krzek
authors.forename: Anna
authors.initials: A
authors.lastname: Sajdak
mesh_terms: D014150:Antipsychotic Agents / Q000032:analysis*; D005476:Fluphenazine / Q000032:analysis; D006220:Haloperidol / Q000032:analysis; D010464:Perazine / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011395:Promazine / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D014150:Antipsychotic Agents; D004364:Pharmaceutical Preparations; D010464:Perazine; D006220:Haloperidol; D011395:Promazine; D005476:Fluphenazine