Screening and identification of putative allergens in berry fruits of the Rosaceae family: technical challenges.
Cross-reactive proteins in small fruits of the Rosaceae family like strawberry, raspberry and blackberry revealed an unexpected complex IgE-reactivity pattern. Several copies of PR-10 and PR-14 proteins were detected by Southern blots in strawberry, raspberry and blackberry. In raspberry, the highest similarity at the DNA level for PR-10 and PR-14 (Rub i 1 and Rub i 3) was detected to strawberry sequences of Fra a 1 and Fra a 3. At the protein level, Rub i 1 and Rub i 3 showed more than 70% identity with homologous proteins of rosaceous fruits. Furthermore, raspberries contained additional putative allergens, e.g. class III acidic chitinases and cyclophilins. Blackberries were shown to share at least two well-known major fruit allergens with other rosaceous fruits, namely PR-10s and PR-14s homologous proteins. However the IgE-reactive proteins of small fruits are still not extensively investigated. The main challenges in studying small fruit allergens are the complexity of the fruit matrix, the diversity of physico-chemical properties of fruit proteins, the lack of appropriate protein extraction procedures and the missing information about the influence of processing treatments on food components.
Marzban
Gorji
G
Plant Biotechnology Unit, Institute of Applied Microbiology, Department of Biotechnology, BOKU, Vienna, Austria.
Maghuly
Fatemeh
F
Herndl
Anita
A
Katinger
Hermann
H
Laimer
Margit
M
D000485:Allergens / Q000032:analysis* / Q000276:immunology; D015139:Blotting, Southern / Q000379:methods; D005638:Fruit / Q000737:chemistry* / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D027824:Rosaceae / Q000737:chemistry* / Q000276:immunology
D000485:Allergens; D007073:Immunoglobulin E