title: p.Asn176Lys and p.Met137Thr dimorphisms of the PRNP gene significantly decrease the susceptibility to classical scrapie in ARQ/ARQ sheep.
abstract: In this study, we investigated the susceptibility to scrapie of Sarda breed sheep carrying the genotype ARQ/ARQ with additional polymorphisms at the PRNP gene. To do this, we examined 256 scrapie-affected sheep and 320 flock-mate negative controls from 24 flocks. Logistic regression analysis demonstrated that sheep carrying the ARQ/ARQ genotype with additional dimorphisms had lower risk of becoming scrapie affected when compared with those with ARQ/ARQ(wildtype) genotype. ARQ/ARQ genotypes that were detected with heterozygous or homozygous p.Asn176Lys and p.Met137Thr dimorphisms were associated with the lowest susceptibility to the disease. A significant lower risk was also associated with the p.Arg154His dimorphism, while p.Leu141Phe had a protective effect that was not statistically significant.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sardegna, Via Duca degli Abruzzi 8, 07100 Sassari, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Maestrale
authors.forename: A
authors.initials: A
authors.lastname: Carta
authors.forename: S
authors.initials: S
authors.lastname: Attene
authors.forename: A
authors.initials: A
authors.lastname: Galistu
authors.forename: C
authors.initials: C
authors.lastname: Santucciu
authors.forename: M G
authors.initials: MG
authors.lastname: Cancedda
authors.forename: M
authors.initials: M
authors.lastname: Saba
authors.forename: S
authors.initials: S
authors.lastname: Sechi
authors.forename: C
authors.initials: C
authors.lastname: Patta
authors.forename: E
authors.initials: E
authors.lastname: Bandino
authors.forename: C
authors.initials: C
authors.lastname: Ligios
mesh_terms: D000818:Animals; D020022:Genetic Predisposition to Disease; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics*; D012608:Scrapie / Q000235:genetics*; D012756:Sheep
chemical_list: D011328:Prions