Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells.
Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microg/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.
Soliman
Mohamed Mohamed
MM
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 020-013, Egypt. mohamedsoliman8896@yahoo.com
El-Senosi
Yakut Abdel-Fattah
YA
Salem
Maysara Mahmoud
MM
Abdel Hamid
Omniya Mahmoud
OM
Kazuhiro
Kimura
K
D016475:3T3 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D003176:Complement C3; D005786:Gene Expression Regulation / Q000187:drug effects*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D011480:Protease Inhibitors / Q000494:pharmacology*; D013997:Time Factors
C545610:C3 protein, mouse; D003176:Complement C3; D007004:Hypoglycemic Agents; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D011480:Protease Inhibitors
J Anim Sci. 2002 Mar;80(3):751-6
11890412
Atherosclerosis. 1990 Mar;81(2):111-8
2322321
Curr Opin Genet Dev. 1995 Oct;5(5):571-6
8664544
Arterioscler Thromb Vasc Biol. 2001 Jun;21(6):1034-9
11397716
Metabolism. 1993 May;42(5):574-80
8492712
Eur J Clin Invest. 1995 Nov;25(11):817-25
8582446
J Pharmacol Exp Ther. 2007 Jan;320(1):291-9
17038510
J Biol Chem. 1992 Jun 25;267(18):12736-41
1618777
AIDS. 1998 Oct 22;12(15):F167-73
9814858
J Biol Chem. 1989 Jan 5;264(1):426-30
2909530
Diabetes. 2001 Jun;50(6):1378-88
11375339
Lancet. 1998 Jun 20;351(9119):1881-3
9652687
AIDS. 1998 May 7;12(7):F51-8
9619798
J Biol Chem. 2004 Feb 6;279(6):4051-7
14615480
Nutr Metab (Lond). 2007 May 09;4:11
17490487
Diabetes Metab. 1999 Sep;25(3):225-32
10499191
J Clin Endocrinol Metab. 1999 Nov;84(11):4274-7
10566684
Science. 1987 Jul 24;237(4813):402-5
3299705
J Lipid Res. 2006 Mar;47(3):643-52
16333141
J Biol Chem. 1999 Mar 5;274(10):6718-25
10037770