Human endogenous retrovirus-R Env glycoprotein as possible autoantigen in autoimmune disease.
It has long been discussed whether endogenous retroviruses (ERVs) are involved in the pathogenesis of autoimmune diseases. Among various human endogenous retroviruses (HERVs), we have focused on HERV-R. To investigate the biological roles of HERV-R, we earlier established transgenic rats carrying the full sequence of the viral genome. In these HERV-R rats, however, no disease occurred. Another trigger that induces autoimmunity may be essential for the recognition of HERV-R products by the immune system. Thus, in this study, we mated HERV-R rats with env-pX rats (transgenic rats carrying the env-pX gene of human T cell leukemia virus type I) that develop autoimmune diseases, and generated double transgenic (DTG) rats. In DTG rats, autoimmune diseases occurred similarly in env-pX rats. Interestingly, deposition of rat IgM but not IgG was observed on the glomerular endothelial cells. Such IgM deposition was never seen in the parental HERV-R or env-pX rats. We considered that in situ formation of immune complexes consisted of the HERV-R env glycoprotein and anti-HERV-R env IgM antibodies (Abs) in DTG rats, according to the following evidence: (1) No dense deposit, representing deposition of circulating immune complexes, was seen on glomerular endothelial cells. (2) IgM Abs reactive with HERV-R env glycoprotein were generated in the serum. (3) HERV-R env glycoprotein was expressed in the kidney, specifically on glomerular endothelial cells. (4) IgM deposition was partly colocalized with the HERV-R env glycoprotein on the glomeruli. These findings strongly suggest that the HERV-R env glycoprotein is recognized as an autoantigen in the host with autoimmune diseases.
Sasaki
Naomi
N
Department of Pathology, Hokkaido University Graduate School of Medicine, Sapporo 060-0812, Japan.
Ogawa
Yayoi
Y
Iinuma
Chihiro
C
Tomaru
Utano
U
Katsumata
Kazuaki
K
Otsuka
Noriyuki
N
Kasahara
Masanori
M
Yoshiki
Takashi
T
Ishizu
Akihiro
A
D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000209:etiology*; D020077:Endogenous Retroviruses / Q000276:immunology*; D005260:Female; D015686:Gene Products, env / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007678:Kidney Glomerulus / Q000473:pathology; D051381:Rats; D055647:Rats, Transgenic; D016019:Survival Analysis
D001324:Autoantigens; D015686:Gene Products, env; D007075:Immunoglobulin M