title: Polydimethylsiloxane microfluidic chip with integrated microheater and thermal sensor.
abstract: A microheater and a thermal sensor were fabricated inside elastomeric polydimethylsiloxane microchannels by injecting silver paint (or other conductive materials) into the channels. With a high-precision control scheme, microheaters can be used for rapid heating, with precise temperature control and uniform thermal distribution. Using such a microheater and feedback system, a polymerase chain reaction experiment was carried out whereas the DNA was successfully amplified in 25 cycles, with 1 min per cycle.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Wu
authors.forename: Wenbin
authors.initials: W
authors.lastname: Cao
authors.forename: Weijia
authors.initials: W
authors.lastname: Wen
authors.forename: Donald Choy
authors.initials: DC
authors.lastname: Chang
authors.forename: Ping
authors.initials: P
authors.lastname: Sheng
references.citation: Anal Chem. 2003 Jul 15;75(14):3446-50
references.pmid: 14570196
references.citation: Lab Chip. 2001 Dec;1(2):102-7
references.pmid: 15100868
references.citation: Lab Chip. 2003 Nov;3(4):281-6
references.pmid: 15007459
references.citation: Anal Chem. 2004 Jun 1;76(11):3162-70
references.pmid: 15167797
references.citation: Anal Chem. 2004 Mar 1;76(5):1537-43
references.pmid: 14987116
references.citation: Acc Chem Res. 2002 Jul;35(7):491-9
references.pmid: 12118988
references.citation: Nature. 2006 Jul 27;442(7101):394-402
references.pmid: 16871207
references.citation: Electrophoresis. 2003 Jan;24(1-2):172-8
references.pmid: 12652588
references.citation: Lab Chip. 2004 Dec;4(6):563-9
references.pmid: 15570366
references.citation: Biomicrofluidics. 2008 Jul 21;2(3):34103
references.pmid: 19693370
references.citation: Electrophoresis. 2002 May;23(10):1531-6
references.pmid: 12116165
references.citation: Nature. 2003 Mar 6;422(6927):28-9
references.pmid: 12621418
references.citation: Anal Chem. 2008 Apr 1;80(7):2447-51
references.pmid: 18302344
references.citation: Biotechnol Adv. 2006 May-Jun;24(3):243-84
references.pmid: 16326063
references.citation: Anal Chem. 2005 Jun 15;77(12):3887-93
references.pmid: 15952761
references.citation: Anal Chem. 2003 Sep 1;75(17):4591-8
references.pmid: 14632069
references.citation: Lab Chip. 2004 Aug;4(4):401-7
references.pmid: 15269812