title: Asymptomatic carriage of protozoan parasites in children in day care centers in the United kingdom.
abstract: Point prevalence of Cryptosporidium and Giardia carriage among 230 asymptomatic preschool children attending day-care facilities was 1.3% (95% CI: 0.3%-3.8%) for each parasite, with no dual infections. Cryptosporidium oocysts were detected by immunomagnetic separation and immunofluorescence microscopy and genotyped: one isolate was Cryptosporidium hominis, the other 2 being skunk and cervine genotypes, rarely found in symptomatic human infection.
authors.affiliation: Institute of Life Sciences, School of Medicine, Swansea University, Swansea, United Kingdom.
authors.forename: Angharad Puw
authors.initials: AP
authors.lastname: Davies
authors.forename: Brian
authors.initials: B
authors.lastname: Campbell
authors.forename: Meirion Rhys
authors.initials: MR
authors.lastname: Evans
authors.forename: Angie
authors.initials: A
authors.lastname: Bone
authors.forename: Anita
authors.initials: A
authors.lastname: Roche
authors.forename: Rachel Mary
authors.initials: RM
authors.lastname: Chalmers
mesh_terms: D000293:Adolescent; D002353:Carrier State / Q000453:epidemiology* / Q000469:parasitology; D002648:Child; D002656:Child Day Care Centers; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005260:Female; D005872:Giardia / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology* / Q000469:parasitology; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D015995:Prevalence; D017422:Sequence Analysis, DNA; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D016054:DNA, Protozoan