Synthesis and characterization of a redox-active ion channel supporting cation flux in lipid bilayers.
The synthesis, cation binding and transmembrane conductive properties of a novel synthetic ion channel containing a redox-active ferrocene unit are described. Fluorescence spectroscopy was used to demonstrate that the channel supports multiple ion coordination and association constants for 1:1 and 1:2 (channel:cation) coordination for both Na(+) and K(+) were evaluated. Experiments using a black lipid membrane preparation revealed that this compound functioned effectively as an ion channel for both Na(+) and K(+). Concomitant (23)Na NMR spectroscopy studies supported this finding and revealed a Na(+) flux, at least 5 times higher than ion transport rates by monensin. Furthermore, oxidation of the redox-active centre (Fe(2+) to Fe(3+)) effectively inhibited ion transport.
Tsikolia
Maia
M
Center for Heterocyclic Compounds, Department of Chemistry, 126 Sisler Hall, University of Florida, Gainesville, FL 32611-7200, USA.
Hall
Adam C
AC
Suarez
Cristina
C
Nylander
Zazi O
ZO
Wardlaw
Sarah M
SM
Gibson
Molly E
ME
Valentine
Kerry L
KL
Onyewadume
Louisa N
LN
Ahove
Deborah A
DA
Woodbury
Maya
M
Mongare
Margaret M
MM
Hall
C Dennis
CD
Wang
Zuoquan
Z
Draghici
Bogdan
B
Katritzky
Alan R
AR
D001692:Biological Transport; D002412:Cations / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004553:Electric Conductivity; D005296:Ferrous Compounds / Q000737:chemistry; D007473:Ion Channels / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D000075163:Metallocenes; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence
D002412:Cations; D005296:Ferrous Compounds; D007473:Ion Channels; D008051:Lipid Bilayers; D000075163:Metallocenes; C004998:ferrocene