title: A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction.
abstract: Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis.
authors.affiliation: Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará CEP: 60.425-540, Brazil.
authors.forename: Manuela Soares
authors.initials: MS
authors.lastname: Couto
authors.forename: Rossana de Aguiar
authors.initials: Rde A
authors.lastname: Cordeiro
authors.forename: Marcos Fábio Gadelha
authors.initials: MF
authors.lastname: Rocha
authors.forename: Thalles Barbosa
authors.initials: TB
authors.lastname: Grangeiro
authors.forename: Natanael Pinheiro
authors.initials: NP
authors.lastname: Leitão Junior
authors.forename: Tereza de Jesus Pinheiro Gomes
authors.initials: Tde J
authors.lastname: Bandeira
authors.forename: José Júlio Costa
authors.initials: JJ
authors.lastname: Sidrim
authors.forename: Raimunda Sâmia Nogueira
authors.initials: RS
authors.lastname: Brilhante
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014481:United States
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer