title: Mutagenic Activity of Indigofera truxillensis and I. suffruticosa Aerial Parts.
abstract: Indigofera truxillensis and I. suffruticosa, are used as a source of indigo dye and to treat several diseases. The mutagenic activity of the methanolic extracts from aerial parts, glycerolipid, flavonoid and alkaloid fractions of the extract were evaluated by means of Salmonella/microsome assays using TA100, TA98, TA102 and TA97a strains. The methanolic extract of I. truxillensis showed mutagenic activity in the TA98 strain without S9 while glycerolipid fraction was devoid of activity. The flavonoid and alkaloid fractions of both plants showed mutagenicity. Chemical analysis of flavonoid fractions of I. truxillensis and I. suffruticosa resulted in the identification of kaempferol, quercetin and their derivatives. The alkaloid fraction of both the species contained indigo and indirubin and indigo was found mainly responsible for the mutagenic activity.
authors.affiliation: Departamento de Química Orgânica, Instituto de Química de Araraquara, UNESP-São Paulo State University, c.p. 355, CEP 14800-900, Brazil.
authors.forename: Tamara Regina
authors.initials: TR
authors.lastname: Calvo
authors.forename: Cássia Regina Primila
authors.initials: CR
authors.lastname: Cardoso
authors.forename: Adriana Candido
authors.initials: AC
authors.lastname: da Silva Moura
authors.forename: Lourdes Campaner
authors.initials: LC
authors.lastname: Dos Santos
authors.forename: Ilce Mara Syllos
authors.initials: IM
authors.lastname: Colus
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.forename: Eliana Aparecida
authors.initials: EA
authors.lastname: Varanda
references.citation: J Nutr Biochem. 2002 Oct;13(10):572-584
references.pmid: 12550068
references.citation: J Ethnopharmacol. 2004 Dec;95(2-3):393-7
references.pmid: 15507365
references.citation: Toxicology. 2006 Aug 1;225(1):55-63
references.pmid: 16781041
references.citation: Eur J Pharmacol. 2000 Dec 20;410(1):93-100
references.pmid: 11134660
references.citation: Food Chem Toxicol. 1982 Dec;20(6):917-20
references.pmid: 6761249
references.citation: Jpn J Cancer Res. 1991 Oct;82(10):1061-4
references.pmid: 1955374
references.citation: Mutat Res. 1979 Mar;66(3):223-40
references.pmid: 375081
references.citation: Antimicrob Agents Chemother. 1999 Sep;43(9):2328-31
references.pmid: 10471592
references.citation: Mutat Res. 2000 Nov 20;455(1-2):29-60
references.pmid: 11113466
references.citation: Mutagenesis. 1991 Jul;6(4):289-95
references.pmid: 1943719
references.citation: Food Chem Toxicol. 2004 Dec;42(12):2029-35
references.pmid: 15570690
references.citation: Mutat Res. 1982 Aug;97(4):267-81
references.pmid: 6750390
references.citation: Toxicol Lett. 1984 Apr;21(1):119-25
references.pmid: 6372166
references.citation: Mutagenesis. 1986 May;1(3):179-83
references.pmid: 3331657
references.citation: Phytochemistry. 2001 Nov;58(6):897-904
references.pmid: 11684187
references.citation: Mutat Res. 1996 Aug 8;360(3):165-71
references.pmid: 8692215
references.citation: Evid Based Complement Alternat Med. 2007 Sep;4(3):355-9
references.pmid: 17965767
references.citation: Biochemistry. 2000 Nov 14;39(45):13817-24
references.pmid: 11076521
references.citation: Food Chem Toxicol. 2006 Sep;44(9):1585-9
references.pmid: 16730111
references.citation: Chem Res Toxicol. 1991 Jul-Aug;4(4):391-407
references.pmid: 1912325
references.citation: Chem Res Toxicol. 2004 Jun;17(6):814-8
references.pmid: 15206902
references.citation: J Biol Chem. 2001 Jan 5;276(1):251-60
references.pmid: 11013232
references.citation: Science. 1983 Oct 14;222(4620):167-9
references.pmid: 6353574
references.citation: Carcinogenesis. 2000 Mar;21(3):345-51
references.pmid: 10688854
references.citation: Toxicology. 2005 Mar 1;208(1):141-7
references.pmid: 15664441
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2008 May;147(4):409-15
references.pmid: 18291725
references.citation: Phytochemistry. 1996 Nov;43(5):1115-8
references.pmid: 8987878
references.citation: Environ Mol Mutagen. 2003;42(3):144-54
references.pmid: 14556222
references.citation: Environ Mol Mutagen. 1990;15(3):145-82
references.pmid: 2185012
references.citation: Fitoterapia. 2003 Apr;74(3):280-3
references.pmid: 12727494
references.citation: Teratog Carcinog Mutagen. 1996;16(4):229-41
references.pmid: 9381410
references.citation: Evid Based Complement Alternat Med. 2006 Jun;3(2):261-5
references.pmid: 16786057
references.citation: Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5998-6003
references.pmid: 15837920
references.citation: Proc Natl Acad Sci U S A. 1973 Aug;70(8):2281-5
references.pmid: 4151811
references.citation: Cancer Res. 1985 Jul;45(7):3125-9
references.pmid: 4005849
references.citation: Mutat Res. 1978 Dec;54(3):297-309
references.pmid: 368618
references.citation: Planta Med. 2007 Oct;73(12):1241-6
references.pmid: 17973201
references.citation: Biochem Pharmacol. 2004 Jan 1;67(1):167-74
references.pmid: 14667939
references.citation: J Pharm Sci. 1978 Feb;67(2):249-51
references.pmid: 621649
references.citation: Mycopathologia. 1999;146(1):9-12
references.pmid: 10721515
references.citation: Regul Toxicol Pharmacol. 1998 Oct;28(2):85-95
references.pmid: 9927558
references.citation: Dig Dis Sci. 2004 Mar;49(3):384-94
references.pmid: 15139485
references.citation: Mutat Res. 1983 May;113(3-4):173-215
references.pmid: 6341825