[Research on callus induction and cell suspension culture of Changium smyrnioides].
OBJECTIVE
To establish the technical systems of callus induction and the cell suspension culture of Changium smyrnioides.


METHODS
Effects of different plant growth regulators on leaf, petiole and root callus induction were studied by orthogonal design, and cell suspension cultures from different explants were carried out.


RESULTS
Petiole was optimal to form callus, followed by the root and leaf. Four kinds of plant growth regulators showed a significant effect on the petiole and root callus induction. The optimal plant growth regulators for petiole callus induction was 0.5 mg x L(-1) NAA, 0.5 mg x L(-1) 2,4-D, 0.1 mg x L(-1) KT and 0.5 mg x L(-1) 6-BA; and induction rate reached to 100% when petiole was cultured for 14 days. In addition, when cell from petiole callus was cultured for 21 days, the maximum yields of cell biomass and polysaccharide were 1090.28 mg x L(-1) and 36.85 mg x L(-1), respectively.


CONCLUSIONS
Petiole was taken as the optimal explant for inducing callus and the optimal cell origin for the cell suspension culture of C. smyrnioides.
Jiang
Shu
S
Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China. jiangshu1970@yahoo.com.cn
Duan
Jin'ao
J
Chen
Jianwei
J
Tao
Jinhua
J
Fu
Kunhua
K
D018929:Cell Culture Techniques / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D019684:Magnoliopsida / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D010937:Plant Growth Regulators