title: New reusable Celite/ethylene glycol cartridges for selective chromatography of steroids before immunoassay.
abstract: OBJECTIVE
  Preparation of reusable and easy to handle Celite chromatographic columns.
  
  
  METHODS
  Weighting precise Celite quantities in cartridges and introducing ethylene glycol methanol solutions. The chromatographic solvents pass throughout Celite under negative pressure.
  
  
  RESULTS
  These new minicolumns are reusable. The steroid recoveries' coefficients of variation are less than 10%, and the steroid separation is good.
  
  
  CONCLUSIONS
  The reusable Celite cartridge use before steroid immunoassays is easier and less time-consuming than classical glass Celite minicolumns.
authors.affiliation: AP-HP CIB GHU Sud Henri Mondor, Faculté de Médecine, 8 rue du Général Sarrail, Créteil, France. giton@univ-paris12.fr
authors.forename: Frank
authors.initials: F
authors.lastname: Giton
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guéchot
authors.forename: Jean
authors.initials: J
authors.lastname: Fiet
mesh_terms: D002845:Chromatography / Q000379:methods*; D019855:Ethylene Glycol / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D019855:Ethylene Glycol