Nippostrongylus brasiliensis: increase of sialomucins reacting with anti-mucin monoclonal antibody HCM31 in rat small intestinal mucosa with primary infection and reinfection.
Infections with the parasitic helminth, Nippostrongylus brasiliensis, cause changes in rat small intestinal goblet cell mucin, particularly in the peripheral sugar residues of oligosaccharide. These changes may correlate with expulsion. In this study, we examined changes in mucin oligosaccharides caused by primary infection and reinfection with N. brasiliensis, using two monoclonal antibodies, HCM31 and PGM34, that react with sialomucin and sulfomucin, respectively. Enzyme-linked immunosorbent assay of jejunal mucins showed that the relative reactivity of mucins with HCM31, but not PGM34, increased up to 16 days after primary infection and 6 days after reinfection, the times when the worms were expelled from the rats. Immunohistochemical studies confirmed that goblet cells stained with HCM31 greatly increased at the time of worm expulsion. These results indicate that the marked increase observed in HCM31-reactive sialomucins may be related to expulsion of the worms.
Tsubokawa
Daigo
D
Department of Biochemistry, Kitasato University Graduate School of Medical Sciences, 1-15-1, Kitasato, Sagamihara, Kanagawa 228-8555, Japan.
Nakamura
Takeshi
T
Goso
Yukinobu
Y
Takano
Yuko
Y
Kurihara
Makoto
M
Ishihara
Kazuhiko
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D020397:Goblet Cells / Q000378:metabolism; D018928:Immunity, Mucosal; D007150:Immunohistochemistry; D007411:Intestinal Diseases, Parasitic / Q000276:immunology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000469:parasitology; D007583:Jejunum / Q000378:metabolism* / Q000469:parasitology; D007700:Kinetics; D037102:Lectins; D008297:Male; D009559:Nippostrongylus / Q000276:immunology / Q000502:physiology*; D010270:Parasite Egg Count; D051381:Rats; D017208:Rats, Wistar; D052116:Sialomucins / Q000276:immunology / Q000378:metabolism*; D017206:Strongylida Infections / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D037102:Lectins; D052116:Sialomucins