title: Long-term immunogenicity and protection against Mycoplasma agalactiae induced by an oil adjuvant vaccine in sheep.
abstract: The long-term protective immunity of an inactivated mineral-oil adjuvanted Mycoplasma agalactiae vaccine was evaluated in sheep. The antigen suspension was emulsified with a mixture of three mineral oils (Montanide ISA-563, Marcol-52, Montane-80 at the ratio of 30%, 63%, and 7%, respectively). Twenty-two animals were divided in 2 groups (A and B) and immunised with two doses of the vaccine (group A, n=14) or used as unvaccinated control (group B, n=8). Five months after the second vaccination, seven animals of group A and four animals of group B were challenged by nasal route with M. agalactiae. The remaining seven vaccinated and four control animals were challenged intranasally eight months after vaccination. The vaccine was able to induce a full-protective immunity preventing the clinical signs of contagious agalactia and the infection by M. agalactiae in all groups of animals irrespective of the time of challenge after booster administration.
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy. d.buonavoglia@veterinaria.uniba.it
authors.forename: Domenico
authors.initials: D
authors.lastname: Buonavoglia
authors.forename: Grazia
authors.initials: G
authors.lastname: Greco
authors.forename: Marialaura
authors.initials: M
authors.lastname: Corrente
authors.forename: Maria Fiorella
authors.initials: MF
authors.lastname: Greco
authors.forename: Maria
authors.initials: M
authors.lastname: D'Abramo
authors.forename: Francesca
authors.initials: F
authors.lastname: Latronico
authors.forename: Antonio
authors.initials: A
authors.lastname: Fasanella
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D008899:Mineral Oil; D009175:Mycoplasma Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D045644:Mycoplasma agalactiae / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012756:Sheep / Q000276:immunology / Q000382:microbiology; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D008899:Mineral Oil