Radiation stability of proteinase K crystals grown by LB nanotemplate method.
A detailed analysis of structural and intensity changes induced by X-ray radiation is presented for two types of proteinase K crystals: crystal grown by classical hanging drop method and those grown by Langmuir-Blodgett (LB) nanotemplate. The comparison of various parameters (e.g. intensity per sigma ratio, unit-cell volume, number of unique reflections, B-factors) and electron density maps as a function of radiation dose, demonstrates that crystals, grown by the LB nanotemplate method, appear to be more resistant against radiation damage than crystals grown by the classical hanging drop method.
Pechkova
Eugenia
E
Nanoworld Institute, CIRSDNNOB University of Genova, Genoa, Italy.
Tripathi
Shailesh
S
Ravelli
Raimond B G
RB
McSweeney
Sean
S
Nicolini
Claudio
C
D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D019286:Endopeptidase K / Q000737:chemistry*; D004795:Enzyme Stability / Q000528:radiation effects; D018625:Microscopy, Atomic Force; D014965:X-Rays / Q000009:adverse effects
D019286:Endopeptidase K