title: CgOpt1, a putative oligopeptide transporter from Colletotrichum gloeosporioides that is involved in responses to auxin and pathogenicity.
abstract: BACKGROUND
  The fungus Colletotrichum gloeosporioides f. sp. aeschynomene produces high levels of indole-3-acetic acid (IAA) in axenic cultures and during plant infection. We generated a suppression subtractive hybridization library enriched for IAA-induced genes and identified a clone, which was highly expressed in IAA-containing medium.
  
  
  RESULTS
  The corresponding gene showed similarity to oligopeptide transporters of the OPT family and was therefore named CgOPT1. Expression of CgOPT1 in mycelia was low, and was enhanced by external application of IAA. cgopt1-silenced mutants produced less spores, had reduced pigmentation, and were less pathogenic to plants than the wild-type strain. IAA enhanced spore formation and caused changes in colony morphology in the wild-type strain, but had no effect on spore formation or colony morphology of the cgopt1-silenced mutants.
  
  
  CONCLUSIONS
  Our results show that IAA induces developmental changes in C. gloeosporioides. These changes are blocked in cgopt1-silenced mutants, suggesting that this protein is involved in regulation of fungal response to IAA. CgOPT1 is also necessary for full virulence, but it is unclear whether this phenotype is related to auxin.
authors.affiliation: Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel. chaguev@yahoo.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: Chagué
authors.forename: Rudy
authors.initials: R
authors.lastname: Maor
authors.forename: Amir
authors.initials: A
authors.lastname: Sharon
mesh_terms: D000595:Amino Acid Sequence; D020231:Colletotrichum / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D007210:Indoleacetic Acids / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D034622:RNA Interference; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C030737:indoleacetic acid
references.citation: Fungal Genet Biol. 2004 Mar;41(3):317-26
references.pmid: 14761792
references.citation: Appl Environ Microbiol. 2004 Mar;70(3):1852-4
references.pmid: 15006816
references.citation: Eukaryot Cell. 2008 Jul;7(7):1098-108
references.pmid: 18456860
references.citation: Prikl Biokhim Mikrobiol. 2006 Mar-Apr;42(2):133-43
references.pmid: 16761564
references.citation: Arch Microbiol. 1999 Sep;172(3):157-66
references.pmid: 10460886
references.citation: FEMS Microbiol Lett. 2005 May 1;246(1):125-32
references.pmid: 15869971
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4153-7
references.pmid: 15010530
references.citation: J Mol Biol. 2001 Jan 19;305(3):567-80
references.pmid: 11152613
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Tree Physiol. 2001 May;21(8):533-40
references.pmid: 11359711
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: Plant Physiol. 2002 Jan;128(1):21-9
references.pmid: 11788749
references.citation: Mol Membr Biol. 2001 Jan-Mar;18(1):105-12
references.pmid: 11396605
references.citation: Bioinformatics. 1998;14(4):378-9
references.pmid: 9632836
references.citation: Appl Environ Microbiol. 1998 Dec;64(12):5030-2
references.pmid: 9835603
references.citation: Mol Plant Pathol. 2008 May;9(3):339-55
references.pmid: 18705875
references.citation: Fungal Genet Biol. 2007 Jan;44(1):32-43
references.pmid: 16950636
references.citation: Phytopathology. 2002 Jun;92(6):590-6
references.pmid: 18944254
references.citation: Mol Microbiol. 1998 May;28(4):729-41
references.pmid: 9643541
references.citation: Annu Rev Phytopathol. 2009;47:133-52
references.pmid: 19400643
references.citation: Curr Genet. 1999 Aug;36(1-2):98-104
references.pmid: 10447601
references.citation: Microbiology (Reading). 1997 Feb;143 ( Pt 2):387-396
references.pmid: 9043116