[Fetal bovine serum enhances expression of PEDF in epidermal keratinocytes and dermal fibroblasts].
OBJECTIVE
To investigate the effect of fetal bovine serum (FBS) on expression of pigment epithelium-derived factor (PEDF) in normal epidermal keratinocytes and dermal fibroblasts.


METHODS
Keratinocytes and fibroblasts were incubated with 10% FBS. PEDF protein level in the cells was determined by immunofluorescence and Western blot.


RESULTS
PEDF was localized mostly in the cytoplasm,while some in the nuclei. The distribution of PEDF in cytoplasm was in a granular pattern. 10% FBS increased the expression of PEDF both in keratinocytes and fibroblasts,but histamine and Phorbol 12-myristate 13-acetate (PMA) did not interfere the distribution of PEDF in cells.


CONCLUSIONS
10% FBS can upregulate expression of PEDF in epidermal keratinocytes and dermal fibroblasts.
MAN
Xiao-yong
XY
Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
YAO
Yong-gang
YG
YANG
Xiao-hong
XH
CAI
Sui-qing
SQ
ZHENG
Min
M
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D044967:Serum / Q000502:physiology*; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D015854:Up-Regulation; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor