title: Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G.C.G.C tetrad.
abstract: Short contiguous arrays of variant CTAGGG repeats in the human telomere are unstable in the male germline and somatic cells, suggesting formation of unusual structures by this repeat type. Here, we report on the structure of an intramolecular G-quadruplex formed by DNA sequences containing four human telomeric variant CTAGGG repeats in potassium solution. Our results reveal a new robust antiparallel G-quadruplex fold involving two G-tetrads sandwiched between a G.C base pair and a G.C.G.C tetrad, which could represent a new platform for drug design targeted to human telomeric DNA.
authors.affiliation: Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore.
authors.forename: Kah Wai
authors.initials: KW
authors.lastname: Lim
authors.forename: Patrizia
authors.initials: P
authors.lastname: Alberti
authors.forename: Aurore
authors.initials: A
authors.lastname: Guédin
authors.forename: Laurent
authors.initials: L
authors.lastname: Lacroix
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Riou
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Royle
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
authors.forename: Anh Tuân
authors.initials: AT
authors.lastname: Phan
mesh_terms: D002151:Calorimetry; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D054856:G-Quadruplexes; D014644:Genetic Variation; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011188:Potassium / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet; D016615:Telomere / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011188:Potassium
references.citation: Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):18938-43
references.pmid: 16365301
references.citation: Structure. 1993 Dec 15;1(4):263-82
references.pmid: 8081740
references.citation: Bioorg Med Chem Lett. 2005 Apr 15;15(8):2047-50
references.pmid: 15808465
references.citation: Nucleic Acids Res. 2008 Oct;36(17):5482-515
references.pmid: 18718931
references.citation: Nucleic Acids Res. 2005 Aug 16;33(14):4649-59
references.pmid: 16106044
references.citation: FEBS J. 2007 Jul;274(14):3545-3556
references.pmid: 17561958
references.citation: Methods Enzymol. 2001;338:341-71
references.pmid: 11460557
references.citation: J Am Chem Soc. 2006 Aug 2;128(30):9963-70
references.pmid: 16866556
references.citation: Nucleic Acids Res. 2006;34(19):5715-9
references.pmid: 17040899
references.citation: J Mol Biol. 1998 Sep 25;282(3):637-52
references.pmid: 9737927
references.citation: Nucleic Acids Res. 2006 May 19;34(9):2723-35
references.pmid: 16714449
references.citation: Biochimie. 2008 May;90(5):686-96
references.pmid: 18294461
references.citation: Nucleic Acids Res. 2007;35(19):6517-25
references.pmid: 17895279
references.citation: Nucleic Acids Res. 2007;35(15):4927-40
references.pmid: 17626043
references.citation: Bioorg Med Chem. 2006 Aug 15;14(16):5584-91
references.pmid: 16682210
references.citation: Nucleic Acids Res. 2005 Oct 12;33(18):5851-60
references.pmid: 16221978
references.citation: J Biomol NMR. 2000 Feb;16(2):175-8
references.pmid: 10723997
references.citation: Biopolymers. 1970;9(9):1059-77
references.pmid: 5449435
references.citation: J Mol Biol. 1998 Sep 25;282(3):619-36
references.pmid: 9737926
references.citation: Nucleic Acids Res. 2005 Jul 21;33(13):4065-77
references.pmid: 16040598
references.citation: Nucleic Acids Res. 2005 Sep 12;33(16):e138
references.pmid: 16157860
references.citation: J Am Chem Soc. 2009 Apr 1;131(12):4301-9
references.pmid: 19271707
references.citation: J Am Chem Soc. 2007 Sep 12;129(36):11185-91
references.pmid: 17705383
references.citation: Nucleic Acids Res. 1989 Jun 26;17(12):4611-27
references.pmid: 2664709
references.citation: J Mol Biol. 2000 May 26;299(1):123-44
references.pmid: 10860727
references.citation: Nucleic Acids Res. 2006 May 08;34(8):2386-97
references.pmid: 16682446
references.citation: Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14629-34
references.pmid: 14645716
references.citation: J Am Chem Soc. 2005 Jul 6;127(26):9439-47
references.pmid: 15984871
references.citation: EMBO J. 1995 Nov 1;14(21):5433-43
references.pmid: 7489732
references.citation: Nucleic Acids Res. 2009 Oct;37(18):6225-38
references.pmid: 19656953
references.citation: Proc Natl Acad Sci U S A. 1988 Sep;85(18):6622-6
references.pmid: 3413114
references.citation: Nucleic Acids Res. 1997 Dec 1;25(23):4758-63
references.pmid: 9365253
references.citation: Nucleic Acids Res. 2005 Jun 02;33(10):3185-92
references.pmid: 15933211
references.citation: Nucleic Acids Res. 2008 Jul;36(12):4079-87
references.pmid: 18535007
references.citation: FEBS Lett. 1998 Sep 11;435(1):74-8
references.pmid: 9755862
references.citation: J Am Chem Soc. 2002 Feb 20;124(7):1160-1
references.pmid: 11841271
references.citation: Nature. 2002 Jun 20;417(6891):876-80
references.pmid: 12050675
references.citation: Nucleic Acids Res. 2005 Apr 07;33(6):2022-31
references.pmid: 15817566
references.citation: Nucleic Acids Res. 2003 Mar 15;31(6):1605-13
references.pmid: 12626701
references.citation: Eur J Cancer. 1997 Apr;33(5):735-49
references.pmid: 9282112
references.citation: Nucleic Acids Res. 2009 Feb;37(3):931-8
references.pmid: 19103662
references.citation: Oligonucleotides. 2003;13(6):515-37
references.pmid: 15025917
references.citation: J Mol Biol. 1995 Dec 8;254(4):638-56
references.pmid: 7500339
references.citation: J Mol Biol. 2001 Oct 5;312(5):1073-88
references.pmid: 11580251
references.citation: J Magn Reson. 2003 Jan;160(1):65-73
references.pmid: 12565051
references.citation: Nucleic Acids Res. 2007;35(9):3064-75
references.pmid: 17452368
references.citation: Nucleic Acids Res. 2007;35(7):2440-50
references.pmid: 17395643
references.citation: Biochimie. 2008 Jan;90(1):131-55
references.pmid: 17822826