In vitro cultivation of a Theileria species from a roan antelope (Hippotragus equinus).
Here we describe the in vitro isolation, propagation, and characterization of a Theileria species from roan antelope (Hippotragus equinus). Cultures were initiated using parts of a prescapular lymph node of an infected roan antelope. After 16 days of culture propagation, the first subculture was carried out; thereafter, subcultures were carried out twice a week. Standard methods for the cultivation of Theileria macroschizonts were applied. DNA was extracted from culture material and a partial polymerase chain reaction amplification of the 18S ribosomal RNA (rRNA) gene was carried out using Theileria genus-specific primers. It has been shown that Theileria sp. (roan) had high levels of nucleic acid identity with sequence data of the 18S rRNA gene of a Theileria sp. previously isolated from a sable antelope. The phylogenetic analysis showed that this isolate is closely related to several undescribed Theileria spp. which have previously been identified from a dog and some other antelope species in South Africa.
Zweygarth
Erich
E
Onderstepoort Veterinary Institute, Onderstepoort, South Africa. ZweygarthE@gmail.com
Benade
Justin
J
Steyl
Johan
J
Prozesky
Leon
L
Koekemoer
Otto
O
Josemans
Antoinette I
AI
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D008198:Lymph Nodes / Q000469:parasitology; D010274:Parasitology / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000302:isolation & purification; D012418:Ruminants / Q000469:parasitology*; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid; D013019:South Africa; D016794:Theileria / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*
D017931:DNA Primers; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S
J Mol Evol. 1980 Dec;16(2):111-20
7463489
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Parasitology. 1993 Aug;107 ( Pt 2):157-65
8414670
Res Vet Sci. 1980 Sep;29(2):219-29U
7193340
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791
J Clin Microbiol. 2005 Dec;43(12):5907-11
16333074
J Med Res. 1912 Jun;26(2):195-218.5
19972051