title: Multiple Rieske proteins enable short- and long-term light adaptation of Synechocystis sp. PCC 6803.
abstract: In contrast to eukaryotes, most cyanobacteria contain several isoforms of the Rieske iron-sulfur protein, PetC, resulting in heterogeneity in the composition of the cytochrome b(6)f complexes. Of three isoforms in the mesophilic cyanobacterium Synechocystis PCC 6803, PetC1 is the major Rieske protein in the cytochrome b(6)f complex, whereas the physiological function of PetC2 and PetC3 is still uncertain. Comparison of wild type and various petC-deficient strains under selected light conditions revealed distinct functional differences: high-light exposure of wild type cells resulted in a significantly enhanced petC2 transcript level, whereas a Delta petC1 mutant showed a low cytochrome b(6)f content, low electron flux, and a considerably increased accumulation of cytochrome-bd oxidase. In contrast to wild type and Delta petC1, Delta petC2 and Delta petC3 strains still grew fast under high-light conditions although all three Rieske proteins are required for maximal electron transport rates. Although the presence of PetC3 appears to be required for activation of the cyclic electron transport, state transitions were more effective in the absence of PetC2 and/or PetC3. In summary, our data suggest defined roles of the various PetC proteins in short- and long-term light adaptation.
authors.affiliation: Department of Plant Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany; Division of Biology, Radioisotope Research Center, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tsunoyama
authors.affiliation: Department of Plant Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.
authors.forename: Gábor
authors.initials: G
authors.lastname: Bernát
authors.affiliation: Department of Plant Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.
authors.forename: Nina G
authors.initials: NG
authors.lastname: Dyczmons
authors.affiliation: Department of Biochemistry and Molecular Biology, ZBMZ, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Schneider
authors.affiliation: Department of Plant Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany. Electronic address: Matthias.Roegner@rub.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Rögner
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D045346:Cytochrome b6f Complex / Q000378:metabolism; D004579:Electron Transport / Q000502:physiology; D014450:Electron Transport Complex III / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008027:Light; D010084:Oxidation-Reduction; D010788:Photosynthesis / Q000502:physiology*; D010798:Phycocyanin / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D020033:Protein Isoforms; D021122:Protein Subunits; C037989:Rieske iron-sulfur protein; C002497:allophycocyanin; D010798:Phycocyanin; D002734:Chlorophyll; D045346:Cytochrome b6f Complex; D014450:Electron Transport Complex III
references.citation: Microbiol Rev. 1993 Sep;57(3):725-49
references.pmid: 8246846
references.citation: J Biochem. 2009 Mar;145(3):395-401
references.pmid: 19124292
references.citation: Annu Rev Plant Biol. 2007;58:199-217
references.pmid: 17201689
references.citation: J Biol Chem. 2004 Oct 22;279(43):44621-7
references.pmid: 15316016
references.citation: EMBO J. 2001 Jul 16;20(14):3623-30
references.pmid: 11447103
references.citation: Photosynth Res. 2008 Feb-Mar;95(2-3):175-82
references.pmid: 17922214
references.citation: Biochim Biophys Acta. 2006 May-Jun;1757(5-6):362-8
references.pmid: 16762315
references.citation: Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1585-90
references.pmid: 11038603
references.citation: J Exp Bot. 2005 Jan;56(411):389-93
references.pmid: 15582926
references.citation: Plant Cell. 1999 Oct;11(10):2031-44
references.pmid: 10521530
references.citation: Plant Cell. 2006 Nov;18(11):3106-20
references.pmid: 17114352
references.citation: DNA Res. 1996 Jun 30;3(3):109-36
references.pmid: 8905231
references.citation: Biochim Biophys Acta. 1969 Feb 25;172(2):242-51
references.pmid: 5775694
references.citation: Res Microbiol. 2007 Jan-Feb;158(1):45-50
references.pmid: 17224258
references.citation: Nucleic Acids Res. 2002 May 1;30(9):e36
references.pmid: 11972351
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Mol Cell Proteomics. 2002 Oct;1(10):816-27
references.pmid: 12438564
references.citation: Curr Opin Plant Biol. 1998 Jun;1(3):217-23
references.pmid: 10066592
references.citation: FEBS Lett. 2002 May 22;519(1-3):82-6
references.pmid: 12023022
references.citation: J Biol Chem. 2007 Feb 9;282(6):3730-7
references.pmid: 17166849
references.citation: Trends Plant Sci. 2004 Mar;9(3):130-7
references.pmid: 15003236
references.citation: Biochemistry. 2002 Mar 12;41(10):3422-9
references.pmid: 11876651
references.citation: J Biol Chem. 2002 Mar 29;277(13):10949-54
references.pmid: 11788579
references.citation: J Biol Chem. 1995 Oct 20;270(42):25225-32
references.pmid: 7559660
references.citation: Plant Cell Physiol. 2005 Aug;46(8):1433-6
references.pmid: 15946981
references.citation: EMBO Rep. 2002 Mar;3(3):280-5
references.pmid: 11850400
references.citation: Photosynth Res. 2004;82(3):327-38
references.pmid: 16143844
references.citation: Biochim Biophys Acta. 2009 Dec;1787(12):1479-85
references.pmid: 19577535
references.citation: J Biol Chem. 2001 May 18;276(20):16780-5
references.pmid: 11278512
references.citation: Photosynth Res. 1993 Jun;36(3):149-68
references.pmid: 24318920
references.citation: J Biol Chem. 2004 Sep 17;279(38):39383-8
references.pmid: 15262969
references.citation: J Struct Biol. 1998 Dec 15;124(2-3):311-34
references.pmid: 10049814
references.citation: Biochim Biophys Acta. 1969;189(3):366-83
references.pmid: 5370012
references.citation: J Biol Chem. 2003 Jun 6;278(23):20925-33
references.pmid: 12672829
references.citation: Annu Rev Biochem. 2006;75:769-90
references.pmid: 16756511
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Biochemistry. 1998 Dec 22;37(51):17944-51
references.pmid: 9922162
references.citation: EMBO J. 1999 Jun 1;18(11):2961-9
references.pmid: 10357809
references.citation: Photosynth Res. 2009 Mar;99(3):205-16
references.pmid: 19137411
references.citation: Plant Physiol. 2000 Feb;122(2):505-16
references.pmid: 10677443
references.citation: Mol Microbiol. 2008 Oct;70(1):140-50
references.pmid: 18699865
references.citation: Biochim Biophys Acta. 2007 Feb;1767(2):161-9
references.pmid: 17274945
references.citation: Biochim Biophys Acta. 2006 May-Jun;1757(5-6):339-45
references.pmid: 16787635
references.citation: Nature. 2004 Jun 3;429(6991):579-82
references.pmid: 15175756
references.citation: Biochim Biophys Acta. 2005 Nov 15;1710(1):1-12
references.pmid: 16271700
references.citation: EMBO J. 1994 Mar 1;13(5):1019-27
references.pmid: 8131736
references.citation: Plant Cell Environ. 2007 Sep;30(9):1041-51
references.pmid: 17661746
references.citation: J Biol Chem. 2001 Oct 12;276(41):38159-65
references.pmid: 11483610
references.citation: J Mol Endocrinol. 2002 Aug;29(1):23-39
references.pmid: 12200227
references.citation: Science. 2003 Mar 7;299(5612):1530-2
references.pmid: 12624254
references.citation: Photosynth Res. 2004;82(1):17-33
references.pmid: 16228610
references.citation: J Cell Biol. 1973 Aug;58(2):419-35
references.pmid: 4199659
references.citation: Trends Plant Sci. 2006 Jan;11(1):46-55
references.pmid: 16352458
references.citation: J Biol Chem. 1988 Oct 5;263(28):14334-42
references.pmid: 2844767
references.citation: Neurosci Lett. 2003 Mar 13;339(1):62-6
references.pmid: 12618301