Recent advances in mass spectrometry-based peptidome analysis.
The peptidome, which is the low-molecular-weight subset of the proteome, has attracted increasing attention in recent years. However, with the interference of high-abundance protein components in complex biological mixtures (e.g., serum), selective extraction of endogenous peptides is the first and most important step before analyzing the peptidome. A number of methods and technologies have now been developed for the selective enrichment, fractionation, quantitative analysis of the endogenous peptides and their application in the potential biomarker discovery. This review will cover the methods and technologies developed in recent years for the peptidome analysis on the selective extraction, multidimensional separation and quantitative analysis, as well as their application for clinical diagnosis and biomarker discovery. The future prospects of the peptidome are also discussed.
Hu
Lianghai
L
CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Ye
Mingliang
M
Zou
Hanfa
H
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D010455:Peptides / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000639:trends
D015415:Biomarkers; D010455:Peptides