title: FcepsilonRIalpha gene -18483A>C polymorphism affects transcriptional activity through YY1 binding.
abstract: Three frequent genetic polymorphisms in the human high-affinity IgE receptor alpha-subunit (FcepsilonRIalpha) were shown to be associated with allergic disorders and/or total serum IgE levels in allergic patients. Two of these were previously demonstrated to affect FcepsilonRIalpha expression while the third -18483A>C (rs2494262) has not yet been subjected to functional studies. We hypothesized that the -18483A>C variant affects transcriptional activity of the FcepsilonRIalpha distal promoter in monocytes in which FcepsilonRIalpha transcription is driven through that regulatory region. Indeed, we confirmed preferential binding of the YY1 transcription factor to the -18483C allele, resulting in lower transcriptional activity when compared with the -18483A allele.
authors.affiliation: Atopy (Allergy) Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Potaczek
authors.forename: Keiko
authors.initials: K
authors.lastname: Maeda
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Wang
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nakano
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kanada
authors.forename: Ewa
authors.initials: E
authors.lastname: Stepien
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Branicka
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Fukai
authors.forename: Mutsuko
authors.initials: M
authors.lastname: Hara
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tokura
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Anetta
authors.initials: A
authors.lastname: Undas
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
mesh_terms: D044466:Asian People / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017455:Receptors, IgE / Q000235:genetics*; D014158:Transcription, Genetic; D044465:White People / Q000235:genetics; D050986:YY1 Transcription Factor / Q000378:metabolism*
chemical_list: C543355:FcepsilonRI alpha-chain, human; D017455:Receptors, IgE; D050986:YY1 Transcription Factor
references.citation: Allergy. 2008 May;63(5):626-7
references.pmid: 18394141
references.citation: Allergy. 2006 Oct;61(10):1230-3
references.pmid: 16942574
references.citation: J Immunol. 2003 Aug 15;171(4):1927-33
references.pmid: 12902495
references.citation: J Immunol. 2004 Nov 15;173(10):6458-64
references.pmid: 15528387
references.citation: Am J Respir Crit Care Med. 2004 Jan 15;169(2):214-9
references.pmid: 14597484
references.citation: Ann Allergy Asthma Immunol. 2007 May;98(5):500-1
references.pmid: 17521040
references.citation: Bioinformatics. 2007 Apr 15;23(8):1038-9
references.pmid: 17308338
references.citation: Immunol Res. 2007;37(1):1-16
references.pmid: 17496343
references.citation: Bioinformatics. 2005 Jan 15;21(2):263-5
references.pmid: 15297300
references.citation: Biochem Biophys Res Commun. 2001 Jun 22;284(4):1056-64
references.pmid: 11409901
references.citation: Immunogenetics. 2000 Nov;52(1-2):121-8
references.pmid: 11132148
references.citation: J Appl Physiol (1985). 2002 Jul;93(1):37-41
references.pmid: 12070183
references.citation: J Immunol. 2002 May 1;168(9):4546-52
references.pmid: 11971001
references.citation: J Immunol. 2003 Apr 1;170(7):3732-8
references.pmid: 12646639
references.citation: Immunogenetics. 2008 Oct;60(10):557-63
references.pmid: 18629488
references.citation: J Allergy Clin Immunol. 1999 Jun;103(6):1125-35
references.pmid: 10359895
references.citation: Mol Cell Biol. 1994 Jan;14(1):116-27
references.pmid: 8264580
references.citation: J Biol Chem. 2001 Dec 28;276(52):48871-8
references.pmid: 11687571
references.citation: J Immunol. 2008 Jun 15;180(12):8204-10
references.pmid: 18523286
references.citation: Nucleic Acids Res. 1994 Dec 11;22(24):5151-5
references.pmid: 7816599
references.citation: Allergy. 2007 Sep;62(9):1095-6
references.pmid: 17686114
references.citation: Blood. 2006 Jul 1;108(1):262-9
references.pmid: 16522818
references.citation: J Allergy Clin Immunol. 2007 Feb;119(2):449-56
references.pmid: 17125826