title: Influence of prion strain on prion protein adsorption to soil in a competitive matrix.
abstract: It is likely that the soil environment serves as a stable reservoir of infectious chronic wasting disease (CWD) and scrapie prions, as well as a potential reservoir of bovine spongiform encephalopathy (BSE, or "mad cow" disease). Prion adsorption to soil may play an important role in prion mobility, proteolysis, and infectivity. Differences in PrP environmental fate are possible due to the strain- and species-dependent structure of PrP(Sc). Kinetic and isothermal studies of PrP adsorption to sand and two whole soils were conducted using HY and DY TME-infected hamster, uninfected hamster, and CWD-infected elk brain homogenates as competitive PrP sources. The role of the N-terminus in PrP adsorption was also investigated. We report strain and species differences in PrP adsorption to soil over time and as a function of aqueous concentration, indicating that the fate of prions in the environment may vary with the prion strain and species infected. Our data also provide evidence that the N-terminal region of PrP enhances adsorption to clay but may hinder adsorption to sand. PrP adsorption was maximal at an intermediate aqueous concentration, most likely due to the competitive brain homogenate matrix in which it enters the soil environment.
authors.affiliation: Department of Civil Engineering, University of Nebraska-Lincoln, Peter Kiewit Institute, Omaha, Nebraska, USA.
authors.forename: Samuel E
authors.initials: SE
authors.lastname: Saunders
authors.forename: Jason C
authors.initials: JC
authors.lastname: Bartz
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Bartelt-Hunt
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D006801:Humans; D017096:Prion Diseases / Q000635:transmission / Q000662:veterinary; D011328:Prions / Q000737:chemistry* / Q000235:genetics; D020033:Protein Isoforms / Q000737:chemistry* / Q000235:genetics; D012987:Soil / Q000032:analysis*
chemical_list: D011328:Prions; D020033:Protein Isoforms; D012987:Soil
references.citation: Environ Sci Technol. 2007 Feb 1;41(3):811-7
references.pmid: 17328187
references.citation: Prion. 2009 Jan-Mar;3(1):44-8
references.pmid: 19372734
references.citation: PLoS One. 2007 May 09;2(5):e435
references.pmid: 17502917
references.citation: Nature. 2005 Sep 8;437(7056):257-61
references.pmid: 16148934
references.citation: Bull Environ Contam Toxicol. 1995 May;54(5):668-75
references.pmid: 7780208
references.citation: J Infect Dis. 1982 Nov;146(5):657-64
references.pmid: 6813384
references.citation: Prion. 2008 Oct-Dec;2(4):162-9
references.pmid: 19242120
references.citation: J Virol. 1994 Dec;68(12):7859-68
references.pmid: 7966576
references.citation: J Gen Virol. 2008 Oct;89(Pt 10):2642-2650
references.pmid: 18796735
references.citation: Environ Sci Technol. 2008 Sep 1;42(17):6573-9
references.pmid: 18800532
references.citation: Emerg Infect Dis. 2004 Jun;10(6):1003-6
references.pmid: 15207049
references.citation: J Virol. 2007 Jan;81(2):689-97
references.pmid: 17079313
references.citation: PLoS Pathog. 2007 Jul;3(7):e93
references.pmid: 17616973
references.citation: PLoS One. 2007 Oct 24;2(10):e1069
references.pmid: 17957252
references.citation: J Am Soc Mass Spectrom. 2007 Jun;18(6):1070-9
references.pmid: 17446085
references.citation: Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9741-6
references.pmid: 17535913
references.citation: PLoS Pathog. 2006 Apr;2(4):e32
references.pmid: 16617377
references.citation: J Gen Virol. 1999 Oct;80 ( Pt 10):2757-2764
references.pmid: 10573172
references.citation: J Virol. 2007 May;81(9):4482-91
references.pmid: 17301140
references.citation: Vet Pathol. 2008 Jan;45(1):7-11
references.pmid: 18192568
references.citation: Environ Sci Technol. 2006 Mar 1;40(5):1497-503
references.pmid: 16568762
references.citation: J Gen Virol. 1997 Apr;78 ( Pt 4):975-8
references.pmid: 9129673
references.citation: J Biomater Sci Polym Ed. 1991;3(1):49-67
references.pmid: 1786243
references.citation: Nature. 2003 Sep 4;425(6953):35-6
references.pmid: 12955129
references.citation: Environ Sci Technol. 2008 Feb 1;42(3):728-33
references.pmid: 18323094
references.citation: Environ Sci Technol. 2007 Apr 1;41(7):2324-30
references.pmid: 17438782
references.citation: Biomacromolecules. 2005 Nov-Dec;6(6):3425-32
references.pmid: 16283775
references.citation: Environ Sci Technol. 2006 Oct 15;40(20):6324-9
references.pmid: 17120560
references.citation: Nat Med. 1998 Oct;4(10):1157-65
references.pmid: 9771749
references.citation: Biochem Biophys Res Commun. 2008 Mar 7;367(2):323-9
references.pmid: 18174023
references.citation: Environ Sci Technol. 2009 Jul 15;43(14):5242-8
references.pmid: 19708348