title: Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato.
abstract: DNA mismatch repair proteins play an essential role in maintaining genomic integrity during replication and genetic recombination. We successfully isolated a full length MSH2 and partial MSH7 cDNAs from tomato, based on sequence similarity between MutS and plant MSH homologues. Semi-quantitative RT-PCR reveals higher levels of mRNA expression of both genes in young leaves and floral buds. Genetic mapping placed MSH2 and MSH7 on chromosomes 6 and 7, respectively, and indicates that these genes exist as single copies in the tomato genome. Analysis of protein sequences and phylogeny of the plant MSH gene family show that these proteins are evolutionarily conserved, and follow the classical model of asymmetric protein evolution. Genetic manipulation of the expression of these MSH genes in tomato will provide a potentially useful tool for modifying genetic recombination and hybrid fertility between wide crosses.
authors.affiliation: School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150, Bandar Sunway, Selangor, Malaysia.
authors.forename: Sheh May
authors.initials: SM
authors.lastname: Tam
authors.forename: Sompid
authors.initials: S
authors.lastname: Samipak
authors.forename: Anne
authors.initials: A
authors.lastname: Britt
authors.forename: Roger T
authors.initials: RT
authors.lastname: Chetelat
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D053843:DNA Mismatch Repair / Q000235:genetics*; D005544:Forecasting; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins
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