Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato.
DNA mismatch repair proteins play an essential role in maintaining genomic integrity during replication and genetic recombination. We successfully isolated a full length MSH2 and partial MSH7 cDNAs from tomato, based on sequence similarity between MutS and plant MSH homologues. Semi-quantitative RT-PCR reveals higher levels of mRNA expression of both genes in young leaves and floral buds. Genetic mapping placed MSH2 and MSH7 on chromosomes 6 and 7, respectively, and indicates that these genes exist as single copies in the tomato genome. Analysis of protein sequences and phylogeny of the plant MSH gene family show that these proteins are evolutionarily conserved, and follow the classical model of asymmetric protein evolution. Genetic manipulation of the expression of these MSH genes in tomato will provide a potentially useful tool for modifying genetic recombination and hybrid fertility between wide crosses.
Tam
Sheh May
SM
School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150, Bandar Sunway, Selangor, Malaysia.
Samipak
Sompid
S
Britt
Anne
A
Chetelat
Roger T
RT
D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D053843:DNA Mismatch Repair / Q000235:genetics*; D005544:Forecasting; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins
Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1766-70
17261806
Genetics. 2002 Dec;162(4):1979-93
12524364
Nature. 2000 Oct 12;407(6805):711-7
11048711
Nat Rev Genet. 2001 Jul;2(7):493-503
11433356
Mol Cell. 2004 Aug 13;15(3):437-51
15304223
Nature. 2000 Oct 12;407(6805):703-10
11048710
BMC Genomics. 2006 Oct 19;7:267
17052357
Mamm Genome. 1999 Nov;10(11):1054-61
10556423
Genetics. 1992 Dec;132(4):975-85
1334021
Nucleic Acids Res. 2003 Oct 15;31(20):6027-34
14530450
Annu Rev Microbiol. 2003;57:579-608
14527292
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W197-201
18463136
Nucleic Acids Res. 2000 Jan 15;28(2):463-71
10606644
Annu Rev Genet. 1991;25:229-53
1812808
Theor Appl Genet. 2002 Aug;105(2-3):423-430
12582547
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Genome. 2005 Aug;48(4):685-97
16094436
Mol Gen Genet. 1999 Sep;262(2):239-49
10517319
Proteins. 2005;61 Suppl 7:135-42
16187355
New Phytol. 2005 Dec;168(3):511-28
16313635
Nat Genet. 2000 Jan;24(1):6-8
10615114
Trends Biochem Sci. 2000 Sep;25(9):414-8
10973051
J Mol Evol. 2001 Oct-Nov;53(4-5):436-46
11675603
Mol Genet Genomics. 2001 Jul;265(5):826-36
11523800
Annu Rev Genet. 2000;34:359-399
11092832
Annu Rev Biochem. 1996;65:101-33
8811176
Mol Gen Genet. 1999 Dec;262(4-5):633-42
10628846
Plant Mol Biol. 2004 Oct;56(3):339-49
15604748
Genetics. 1995 Nov;141(3):1147-62
8582620
Genes Dev. 2004 Nov 1;18(21):2676-85
15520284
Cytogenet Genome Res. 2004;107(3-4):146-59
15467360
Nucleic Acids Res. 2007;35(22):7591-603
17965091
Plant Physiol. 1997 Oct;115(2):833-9
9342879
BMC Plant Biol. 2007 Dec 20;7:67
18096080
Genes Dev. 1995 Jul 15;9(14):1728-39
7622037
Genome. 2002 Feb;45(1):116-24
11908653
Theor Appl Genet. 1992 May;83(8):1027-34
24202931
Genome Res. 2003 Sep;13(9):2052-8
12952876
Nucleic Acids Res. 1994 Dec 25;22(25):5723-8
7838728
J Mol Biol. 1990 Jun 20;213(4):583-91
2162963
Chem Rev. 2006 Feb;106(2):302-23
16464007
J Cell Physiol. 2002 Apr;191(1):28-41
11920679
Cell. 1994 Dec 16;79(6):1069-80
8001134
Genes Dev. 2004 Oct 15;18(20):2557-70
15489296
J Mol Evol. 1983;19(2):109-14
6571216
Nucleic Acids Res. 1998 Sep 15;26(18):4291-300
9722651
J Bacteriol. 1994 Sep;176(17):5393-400
8071216
Cell. 1994 Oct 21;79(2):283-92
7954796
Plant Cell. 2000 Jun;12(6):991-1002
10852942
Biochem Soc Trans. 2006 Aug;34(Pt 4):542-4
16856855
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5968-73
12730382
Comput Appl Biosci. 1992 Jun;8(3):275-82
1633570