Classical and non-classical Major Histocompatibility Complex class I gene expression in in vitro derived bovine embryos.
The role of the Major Histocompatibility Complex class I (MHC-I) genes in human and mouse preimplantation embryo development has received considerable attention. In contrast, information concerning the role of these genes in bovine embryo development is limited. The objective of the current study was to characterize the expression pattern of MHC-I genes during preimplantation embryo development in cattle. To this end, bovine oocytes were harvested from slaughterhouse ovaries, matured, fertilized and cultured in vitro. Samples were collected at immature and mature oocyte, presumptive zygote, 2-4-cell embryo, 8-16-cell embryo, morula, blastocyst and hatched blastocyst stages of development. MHC-I expression was detected using quantitative real-time-PCR, cDNA sequencing, whole mount immunocytochemistry and Western blotting. We report classical and non-classical MHC-I mRNA expression in bovine oocytes and developing embryos. Furthermore, we report that the pattern of MHC-I mRNA expression across development was gene- and stage-specific.
Doyle
Julie
J
University College Dublin, Belfield, Ireland.
Ellis
Shirley A
SA
O'Gorman
Grace M
GM
Aparicio Donoso
Ines Maria
IM
Lonergan
Patrick
P
Fair
Trudee
T
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D047108:Embryonic Development / Q000235:genetics / Q000276:immunology; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007120:Immunochemistry; D015778:Minor Histocompatibility Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009028:Morula / Q000276:immunology / Q000378:metabolism*; D009865:Oocytes / Q000276:immunology / Q000378:metabolism*; D009924:Organ Culture Techniques; D015053:Zygote / Q000276:immunology / Q000378:metabolism*
D015415:Biomarkers; D015395:Histocompatibility Antigens Class I; D015778:Minor Histocompatibility Antigens