Identification of novel cyclic nucleotide phosphodiesterase gene cDNAs in the cochlea of guinea pig (Cavia porcellus) through conserved homologous sequences.
The nitric oxide (NO) signaling pathway plays a critical role in auditory signal conversion and transduction. Cyclic nucleotide phosphodiesterase (PDE), an important component of the NO signaling pathway, has not been identified in the cochlea. Using cross-species comparison, homologous sequences of human and mouse Pde coding sequences were searched in a guinea pig genomic database and conserved homologous exons were found between human and mouse homologous sequences. Based on reverse-transcription PCR of these conserved regions, six partial Pde cDNAs were detected in the cochlea: CpPde3a, CpPde4d, CpPde8a, CpPde8b, CpPde9a, and CpPde11a. The identity rates of the six partial Pde cDNA sequences between guinea pig and human range from 83.8 to 95.5% and those of the peptide sequences range from 85.6 to 100%. The identity rates of the six Pde cDNA sequences between guinea pig and mouse range from 80.6 to 93.0% and those of peptide sequences range from 79.5 to 99.2%. The results demonstrate that multiple Pde genes are expressed in the cochlea, suggesting a NO pathway in the auditory system. Insights into this pathway will help to develop new therapeutic drugs on auditory abnormalities.
Huang
Min-Qi
MQ
Institute of Neuroscience, The Second Affiliated Hospital, Guangzhou Medical College, 250 Chang-gang-dong Road, 510260, Guangzhou, China.
Zhang
Jian-Guo
JG
Long
Yue-Sheng
YS
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003051:Cochlea / Q000201:enzymology*; D017124:Conserved Sequence / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D006168:Guinea Pigs / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D015105:3',5'-Cyclic-AMP Phosphodiesterases
Annu Rev Physiol. 1995;57:771-90
7539996
J Comp Neurol. 1999 Feb 1;404(1):52-63
9886024
Pharmacol Rev. 2006 Sep;58(3):488-520
16968949
Brain Res. 1997 Jan 30;747(1):26-33
9042524
Br J Pharmacol. 1998 Dec;125(7):1501-10
9884079
Ann N Y Acad Sci. 2002 May;962:260-74
12076980
Nature. 1983 Nov 10-16;306(5939):174-6
6316142
Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11998-2003
10518565
Annu Rev Physiol. 1995;57:683-706
7539993
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8991-6
9671792
Biochem J. 2003 Feb 15;370(Pt 1):1-18
12444918
J Biol Chem. 1998 Jun 19;273(25):15559-64
9624146
J Allergy Clin Immunol. 2001 Nov;108(5):671-80
11692087
Physiol Rev. 2001 Jan;81(1):209-237
11152758
Annu Rev Genet. 1995;29:305-27
8825477
PLoS Biol. 2007 Sep;5(9):e238
17803358
Hear Res. 2003 Mar;177(1-2):43-52
12618316
Hear Res. 2001 Mar;153(1-2):23-31
11223294
Pharmacol Ther. 2006 Mar;109(3):366-98
16102838
J Biol Chem. 1997 Mar 14;272(11):6823-6
9102399