Capsular polysaccharide production in Enterococcus faecalis and contribution of CpsF to capsule serospecificity.
Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serotypes of E. faecalis were based on immunodetection methods; however, the underlying genetics of capsule production did not fully support these findings. Previously, it was shown that capsule production for serotype C (Maekawa type 2) was dependent on the presence of nine open reading frames (cpsC to cpsK). Using a novel genetic system, we demonstrated that seven of the nine genes in the cps operon are essential for capsule production, indicating that serotypes A and B do not make a capsular polysaccharide. In support of this observation, we showed that serotype C and D capsule polysaccharides mask lipoteichoic acid from detection by agglutinating antibodies. Furthermore, we determined that the genetic basis for the difference in antigenicity between serotypes C and D is the presence of cpsF in serotype C strains. High-pH anion-exchange chromatography with pulsed amperometric detection analysis of serotype C and D capsules indicated that cpsF is responsible for glucosylation of serotype C capsular polysaccharide in E. faecalis.
Thurlow
Lance R
LR
Division of Biology, Kansas State University, 119 Ackert Hall, Manhattan, KS 66506, USA.
Thomas
Vinai Chittezham
VC
Hancock
Lynn E
LE
D016667:Bacterial Capsules / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000235:genetics / Q000502:physiology; D013293:Enterococcus faecalis / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015151:Immunoblotting; D009876:Operon; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012703:Serotyping
D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial
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