Ehrlichia chaffeensis infections in Drosophila melanogaster.
Ehrlichia chaffeensis is an obligate, intracellular bacterium, transmitted by the tick Amblyomma americanum, and is the causative agent of human monocytic ehrlichiosis infections. We previously demonstrated that E. chaffeensis is capable of growing in Drosophila S2 cells. Therefore, we tested the hypothesis that E. chaffeensis can infect adult Drosophila melanogaster. Adult Drosophila organisms were experimentally challenged with intra-abdominal injections of bacteria. Ehrlichia-infected flies showed decreased survival compared to wild-type flies, and bacteria isolated from flies could reinfect mammalian macrophages. Ehrlichia infections activated both the cellular and humoral immune responses in the fly. Hemocytes phagocytosed bacteria after injection, and antimicrobial peptide pathways were induced following infection. Increased pathogenicity in flies carrying mutations in genes in both the Toll and Imd pathways suggests that both immune defense pathways participate in host defense. Induction of Drosophila cellular and humoral responses and the in vivo replication of E. chaffeensis suggests that D. melanogaster is a suitable host for E. chaffeensis. In the future, it will be a useful tool to unlock some of the in vivo mysteries of this arthropod-borne bacterium.
Luce-Fedrow
Alison
A
Kansas State University, Division of Biology, 116 Ackert Hall, Manhattan, KS 66506, USA.
Von Ohlen
Tonia
T
Chapes
Stephen K
SK
D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000276:immunology; D004331:Drosophila melanogaster / Q000276:immunology / Q000382:microbiology*; D018469:Ehrlichia chaffeensis / Q000502:physiology*; D016873:Ehrlichiosis / Q000235:genetics / Q000276:immunology*; D005260:Female; D006790:Host-Parasite Interactions / Q000502:physiology*; D008297:Male; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology
D029721:Drosophila Proteins; D051193:Toll-Like Receptors; C439909:imd protein, Drosophila
PLoS Biol. 2004 Dec;2(12):e418
15562316
Immunity. 2000 Nov;13(5):737-48
11114385
Acta Biol Hung. 2007;58 Suppl:95-111
18297797
Nat Immunol. 2002 Jan;3(1):91-7
11743586
Cell Microbiol. 2008 Jun;10(6):1327-38
18248629
J Biol Chem. 1998 Apr 24;273(17):10463-9
9553105
Nature. 1984 Sep 20-26;311(5983):223-7
6434989
Infect Immun. 2007 Jan;75(1):135-45
17060466
J Immunol. 2006 Aug 15;177(4):2699-706
16888032
Mol Cell Biol. 2007 Jun;27(12):4578-88
17438142
Cell Host Microbe. 2008 May 15;3(5):293-303
18474356
Infect Immun. 2003 Jun;71(6):3540-50
12761139
Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3376-81
10725405
EMBO Rep. 2000 Oct;1(4):353-8
11269502
EMBO J. 1995 Feb 1;14(3):536-45
7859742
Infect Immun. 2008 Aug;76(8):3405-14
18490460
Curr Biol. 2005 Sep 20;15(18):1690-4
16169493
Clin Microbiol Rev. 2003 Jan;16(1):37-64
12525424
EMBO J. 2002 Jun 3;21(11):2568-79
12032070
PLoS Pathog. 2007 Nov;3(11):e173
18039029
Insect Biochem Mol Biol. 2006 Dec;36(12):891-903
17098164
Annu Rev Immunol. 2007;25:697-743
17201680
Appl Environ Microbiol. 2008 Mar;74(6):1886-91
18245255
Curr Biol. 2000 Jun 29;10(13):781-4
10898983
J Biol Chem. 2004 May 14;279(20):21121-7
14985331
Trends Microbiol. 2004 May;12(5):235-42
15120143
FEBS J. 2007 Nov;274(21):5659-68
17922838
Proc Biol Sci. 2007 May 7;274(1614):1211-7
17311779
J Med Entomol. 2004 May;41(3):476-83
15185953
Annu Rev Cell Dev Biol. 1996;12:393-416
8970732
Dev Cell. 2002 Nov;3(5):711-22
12431377
Appl Environ Microbiol. 1999 Feb;65(2):773-8
9925615
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14614-9
9405661
Exp Appl Acarol. 2009 Dec;49(4):347-59
19421877
Infect Immun. 2008 Aug;76(8):3717-24
18490467
J Leukoc Biol. 1995 Jul;58(1):32-9
7616104
Nat Immunol. 2003 Aug;4(8):794-800
12872120
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9369-74
15197269
J Clin Microbiol. 2001 Feb;39(2):460-3
11158090
Mech Dev. 1996 Nov;60(1):109-23
9025065
Cell Microbiol. 2003 Dec;5(12):901-11
14641175
MMWR Morb Mortal Wkly Rep. 2008 Mar 21;55(53):1-92
18354375
J Biol Chem. 2004 Mar 26;279(13):12848-53
14722090
Development. 2001 Dec;128(23):4729-36
11731453
Development. 1991 Sep;113(1):35-54
1765005
Infect Immun. 2003 Sep;71(9):5324-31
12933880
J Cell Sci. 2006 May 15;119(Pt 10):2015-24
16621891
Development. 1991 Jun;112(2):371-88
1794309
Cell. 1996 Sep 20;86(6):973-83
8808632
Infect Immun. 2004 Jan;72(1):498-507
14688131
J Leukoc Biol. 1998 Mar;63(3):297-304
9500516
Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9779-84
19497884
Infect Immun. 2003 Jul;71(7):4059-66
12819096
Vet Res. 2009 Mar-Apr;40(2):34
19358804
Cell Immunol. 1982 Jun;70(1):65-75
7116464
J Mol Diagn. 2005 May;7(2):308-16
15858156
PLoS Pathog. 2007 Mar;3(3):e26
17352533
Science. 2000 Jun 30;288(5475):2376-9
10875925
Cell. 1993 Feb 26;72(4):515-25
8440018
Genes Dev. 1991 May;5(5):797-807
1827421
Cell. 1985 Oct;42(3):779-89
3931918
Vet Microbiol. 1996 Sep;52(1-2):103-12
8914255
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5991-6
12732719
Infect Immun. 2002 Jan;70(1):380-8
11748204