Myocyte stress 1 plays an important role in cellular hypertrophy and protection against apoptosis.
Myocyte stress 1 (MS1) is a recently described striated muscle actin-binding protein that is up-regulated in the early stages of pressure overload left ventricular hypertrophy. The aim of this study was to determine whether MS1 induces cellular hypertrophy and protects against apoptosis. Over-expressed MS1 co-localized with actin in H9c2 cells and altered expression of genes of the myocardin-related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathways and in addition the apoptosis repressor with caspase recruitment domain (Nol3) gene. The size of cells over-expressing MS1 was significantly increased by 55% and over-expression of MS1 dramatically inhibited staurosporine-induced apoptosis by 89%. These findings suggest the involvement of MS1 in cellular hypertrophy and protection against apoptosis.
Koekemoer
Andrea L
AL
Department of Cardiovascular Sciences, University of Leicester, Glenfield General Hospital, Leicester, UK.
Chong
Nelson W
NW
Goodall
Alison H
AH
Samani
Nilesh J
NJ
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000378:metabolism; D006984:Hypertrophy / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008840:Microfilament Proteins; D032342:Muscle Cells / Q000166:cytology / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D019311:Staurosporine / Q000378:metabolism
C461039:Abra protein, rat; D004791:Enzyme Inhibitors; D008840:Microfilament Proteins; D009124:Muscle Proteins; D019311:Staurosporine