Critical ischemic time for the rat ovary: experimental study evaluating early histopathologic changes.
The aim of the present study was to determine a critical ischemic time for ovary in an experimental study in rats. An experimental model using slip-knot tying of all ovarian arteries and veins in cycling female rats was developed. Rat ovaries were tied using the technique through an explorative laparotomy. Complete ischemia times of 1, 2 and 3 h were used for the study. At the end of the ischemic times, the ovaries were harvested following 1 h of reperfusion. Histology indicated a gradually increased congestion correlating with the respective increased ischemic times. According to the present findings 2 h complete ischemia yields a significant injury. The model used in the present study may be used for complete ischemia-reperfusion injury of the rat ovary.
Coskun
Ayhan
A
Department of Obstetrics and Gynecology, Medical Faculty, Sutcu Imam University, Kahramanmaras, Turkey. acoskun@ksu.edu.tr
Coban
Yusuf Kenan
YK
Ciralik
Harun
H
D000818:Animals; D005260:Female; D007511:Ischemia / Q000473:pathology*; D010053:Ovary / Q000098:blood supply*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology