title: Chitinolytic activity of cold tolerant antagonistic species of streptomyces isolated from glacial sites of Indian himalaya.
abstract: Seventy-eight isolates of actinomycetes were isolated from the soil samples collected from alpine zones of Pindari glacier region in Indian Himalaya. Following a plate based rapid screening using two test fungi, five efficient isolates (nos. HA1, HA2, HA6, HA40, and HA142) were selected for further characterization with special reference to their antagonistic properties. Based on phenotypic and genotypic characters, the isolates were identified up to species level. All the isolates belonged to the genus Streptomyces. The isolate nos. HA1 and HA2 were S. sampsonii and HA6, HA40 and HA142 were S. griseobrunneus, S. aurantiacus, and S. griseoluteus, respectively. The isolates showed strong antifungal properties against phytopathogenic test fungi in plate assays. All the isolates hydrolyzed glycol-chitin as a substrate in denaturing conditions showing variable amount of different isoforms.
authors.affiliation: Biotechnological Applications, G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
authors.forename: Mukesh K
authors.initials: MK
authors.lastname: Malviya
authors.forename: Anita
authors.initials: A
authors.lastname: Pandey
authors.forename: Pankaj
authors.initials: P
authors.lastname: Trivedi
authors.forename: Garima
authors.initials: G
authors.lastname: Gupta
authors.forename: Bhavesh
authors.initials: B
authors.lastname: Kumar
mesh_terms: D000898:Antibiosis; D001426:Bacterial Proteins / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism; D003080:Cold Temperature; D005658:Fungi / Q000502:physiology*; D046448:Ice Cover / Q000382:microbiology*; D007194:India; D012988:Soil Microbiology; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology / Q000302:isolation & purification* / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D002686:Chitin; D002688:Chitinases
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