Isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation.
The production of lipase by twenty-nine yeasts isolated from the phylloplane of Hibiscus rosa-sinensis was evaluated. The highest lipase producers were Pseudozyma hubeiensis HB85A, Debaryomyces occidentalis-like HB83 and Cryptococcus sp. HB80. P. hubeiensis HB85A batch fermentations were carried out in a bioreactor and lipase production improved 3.2-fold as compared to flask submerged cultures. The production process was significantly reduced from 48 h (in flasks) to 18 h (in the bioreactor). The better hydrolytic activity was achieved with C16 p-nitrophenyl ester. Maximal activity was observed at pH 7.0, the optimum temperature was 50 degrees C at pH 7.0 and the enzyme was stable at 30 and 40 degrees C. The lipolytic activity was stimulated by Mg(2+), K(+) and Ba(2+) salts and EDTA and slightly inhibited by Ca(2+) salts. Non-ionic detergents such as Triton X-100, Tween 80 and Tween 20 strongly stimulated lipase activity, whereas SDS inhibited it. The lipase was stable in iso-octane and hexane at 80%.
Bussamara
Roberta
R
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil.
Fuentefria
Alexandre Meneghello
AM
de Oliveira
Eder Silva
ES
Broetto
Leonardo
L
Simcikova
Michaela
M
Valente
Patrícia
P
Schrank
Augusto
A
Vainstein
Marilene Henning
MH
D001420:Bacteria, Aerobic / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism*; D010865:Pilot Projects; D013045:Species Specificity
D008049:Lipase