title: Tooth-binding micelles for dental caries prevention.
abstract: Maintenance of the effective local concentration of antimicrobials on the tooth surface is critical for the management of cariogenic bacteria in the oral cavity. We report on the design of a simple tooth-binding micellar drug delivery platform that would effectively bind to tooth surfaces. To achieve tooth-binding ability, the chain termini of biocompatible Pluronic copolymers were modified with a biomineral-binding moiety (i.e., alendronate). The micelles formulated with this polymer were shown to be able to swiftly (<1 min) bind to hydroxyapatite (HA; a model tooth surface) and gradually release the encapsulated model antimicrobial (farnesol). These tooth-binding micelles were negatively charged and had an average effective hydrodynamic diameter of less than 100 nm. In vitro biofilm inhibition studies demonstrated that the farnesol-containing tooth-binding micelles were able to provide significantly stronger inhibition of Streptococcus mutans UA159 biofilm formation on HA discs than the untreated blank control micelles (P < 0.0001). Upon further optimization, this delivery platform could provide an effective tool for caries prevention and treatment.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA.
authors.forename: Fu
authors.initials: F
authors.lastname: Chen
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Liu
authors.forename: Kelly C
authors.initials: KC
authors.lastname: Rice
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Richard A
authors.initials: RA
authors.lastname: Reinhardt
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Bayles
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D018441:Biofilms; D003731:Dental Caries / Q000517:prevention & control*; D016503:Drug Delivery Systems; D017886:Durapatite / Q000378:metabolism; D005204:Farnesol / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008823:Micelles; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D014070:Tooth / Q000378:metabolism*
chemical_list: D008823:Micelles; D005204:Farnesol; D017886:Durapatite
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