title: Efficacy of moxifloxacin or gatifloxacin as prophylaxis against experimental murine Brucella melitensis infection.
abstract: The prophylactic potential of moxifloxacin and gatifloxacin was assessed in comparison with doxycycline, an established therapeutic antibiotic, to limit or control infection by Brucella melitensis in an experimental mouse model, determined by reduced bacterial burden in the spleen. Although moxifloxacin was found to have a small protective effect when administered 6 h following infection, neither moxifloxacin nor gatifloxacin showed significant efficacy in vivo. In comparison, doxycycline provided significant protection when prophylaxis was started at 6 h, 7 days or 14 days following infection. Overall, these results confirm the utility of doxycycline in the prophylaxis of brucellosis and suggest that neither moxifloxacin nor gatifloxacin are likely to be valuable for post-exposure prophylaxis of Brucella infection.
authors.affiliation: Department of Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK. hsatkins@dstl.gov.uk
authors.forename: Helen S
authors.initials: HS
authors.lastname: Atkins
authors.forename: Stephen
authors.initials: S
authors.lastname: Spencer
authors.forename: Simon D
authors.initials: SD
authors.lastname: Brew
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Laws
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Thirlwall
authors.forename: Alastair P
authors.initials: AP
authors.lastname: MacMillan
authors.forename: Timothy J G
authors.initials: TJ
authors.lastname: Brooks
authors.forename: Andrew J H
authors.initials: AJ
authors.lastname: Simpson
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D019072:Antibiotic Prophylaxis / Q000379:methods*; D001372:Aza Compounds / Q000627:therapeutic use*; D017347:Brucella melitensis / Q000187:drug effects; D002006:Brucellosis / Q000382:microbiology / Q000517:prevention & control*; D015169:Colony Count, Microbial; D004318:Doxycycline / Q000627:therapeutic use; D005260:Female; D024841:Fluoroquinolones / Q000627:therapeutic use*; D000077734:Gatifloxacin; D051379:Mice; D008807:Mice, Inbred BALB C; D000077266:Moxifloxacin; D011804:Quinolines / Q000627:therapeutic use*; D013154:Spleen / Q000382:microbiology; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D000077734:Gatifloxacin; D004318:Doxycycline; D000077266:Moxifloxacin