title: Differentiation of Cryptococcus neoformans varieties and Cryptococcus gattii using CAP59-based loop-mediated isothermal DNA amplification.
abstract: Members of the Cryptococcus species complex (C. neoformans and C. gattii) are opportunistic pathogens responsible for frequently fatal cases of meningoencephalitis. These yeasts have been classified into five serotypes. Serotypes A and D are assigned to C. neoformans var. grubii and C. neoformans var. neoformans, respectively, Serotype AD strains are hybrids and serotype B and C strains are considered to belong to the related but distinct species C. gattii. Previous studies have identified 'serotype-associated' alleles of several genes in the Cryptococcus species complex. We developed a loop-mediated isothermal DNA amplification method using CAP59 allele-specific primers to identify the serotypes A, D and B/C of the Cryptococcus species complex.
authors.affiliation: Institute of Hygiene and Tropical Medicine, New University of Lisbon, 1349-008 Lisbon.
authors.forename: S
authors.initials: S
authors.lastname: Lucas
authors.forename: M
authors.initials: M
authors.lastname: da Luz Martins
authors.forename: O
authors.initials: O
authors.lastname: Flores
authors.forename: W
authors.initials: W
authors.lastname: Meyer
authors.forename: I
authors.initials: I
authors.lastname: Spencer-Martins
authors.forename: J
authors.initials: J
authors.lastname: Inácio
mesh_terms: D000483:Alleles; D003453:Cryptococcosis / Q000382:microbiology; D056285:Cryptococcus gattii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D009172:Mycology / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D005656:Fungal Proteins