title: Livin abrogates apoptosis of SPC-A1 cell by regulating JNKI signaling pathway.
abstract: Livin, a novel member of inhibitors of apoptosis protein, is highly expressed in tumor tissues. It is a potential target in tumor therapy. Silencing its gene expression has been found to promote tumor cell apoptosis or increase tumor sensitivity to therapies. This paper studied the effect of livin anti-apoptotic activity and examined its molecular mechanisms. In the study, higher levels of cell apoptosis were measured by FACS in the experiment group with livin expression silenced than that in controls (P < 0.05). After livin gene expression was knocked down, cleaved caspase-3 protein was up-regulated but caspase-3 mRNA expression was almost the same, the phosphorylated JNK1 protein was down-regulated but JNK1 mRNA and total JNK1 protein expression was approximately the same too. The results suggest that livin may exert anti-apoptotic action on SPC-A1 by activating JNK1 signaling pathway and inhibiting caspase-3 activation.
authors.affiliation: Department of Respiratory Medicine, Fujian Provincial Hospital, 350001, Fuzhou, Fujian, People's Republic of China. chenyushengfjsl@yahoo.com.cn
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Hong-Ru
authors.initials: HR
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Lin
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Bao-Song
authors.initials: BS
authors.lastname: Xie
authors.forename: Neng-Luan
authors.initials: NL
authors.lastname: Xu
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Lin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D020935:MAP Kinase Signaling System; D048055:Mitogen-Activated Protein Kinase 8 / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D048055:Mitogen-Activated Protein Kinase 8
references.citation: Cancer Treat Rev. 2007 Apr;33(2):203-12
references.pmid: 17210228
references.citation: Oncogene. 2003 Nov 13;22(51):8330-6
references.pmid: 14614456
references.citation: J Cancer Res Clin Oncol. 2008 Feb;134(2):237-44
references.pmid: 17632732
references.citation: Acta Pharmacol Sin. 2007 Dec;28(12):1968-74
references.pmid: 18031611
references.citation: Cell Mol Life Sci. 2007 May;64(9):1137-44
references.pmid: 17437058
references.citation: Methods. 2002 Feb;26(2):199-213
references.pmid: 12054897
references.citation: Mol Cancer Ther. 2007 Jan;6(1):24-30
references.pmid: 17237263
references.citation: Zhonghua Nan Ke Xue. 2008 Jan;14(1):30-3
references.pmid: 18297808
references.citation: J Biol Chem. 2001 Feb 2;276(5):3238-46
references.pmid: 11024045
references.citation: Nat Rev Genet. 2002 Oct;3(10):737-47
references.pmid: 12360232
references.citation: J Biol Chem. 2006 Feb 10;281(6):3254-60
references.pmid: 16339151
references.citation: Expert Opin Ther Targets. 2008 Apr;12(4):463-76
references.pmid: 18348682
references.citation: Zhonghua Zhong Liu Za Zhi. 2007 Aug;29(8):570-4
references.pmid: 18210873
references.citation: Clin Cancer Res. 2005 Feb 1;11(3):1000-9
references.pmid: 15709165
references.citation: J Biol Chem. 2002 Aug 23;277(34):30454-62
references.pmid: 12048196
references.citation: FEBS Lett. 2001 Apr 20;495(1-2):56-60
references.pmid: 11322947
references.citation: J Virol. 1998 Nov;72(11):8463-71
references.pmid: 9765382
references.citation: Lung Cancer. 2006 Nov;54(2):135-42
references.pmid: 16965834
references.citation: Apoptosis. 2007 Sep;12(9):1543-68
references.pmid: 17573556
references.citation: Ai Zheng. 2007 May;26(5):547-51
references.pmid: 17672950
references.citation: J Mol Med (Berl). 2006 Mar;84(3):232-40
references.pmid: 16437214
references.citation: Cancer Lett. 2007 Jun 8;250(2):168-76
references.pmid: 17218055
references.citation: Urol Oncol. 2009 May-Jun;27(3):277-83
references.pmid: 18555709
references.citation: Tumour Biol. 2008;29(2):76-82
references.pmid: 18515985
references.citation: EMBO J. 1997 Dec 1;16(23):6914-25
references.pmid: 9384571
references.citation: J Biol Chem. 2000 Jul 21;275(29):22064-8
references.pmid: 10807933
references.citation: Blood. 2007 Jan 15;109(2):471-7
references.pmid: 16990595
references.citation: Mol Cell Biol. 2002 Mar;22(6):1754-66
references.pmid: 11865055
references.citation: Cell Death Differ. 2002 Sep;9(9):881-92
references.pmid: 12181739
references.citation: Cell Death Differ. 2006 Feb;13(2):179-88
references.pmid: 16322751