title: A Proteomics Approach Highlights a Myriad of Transporters in the Arabidopsis thaliana Vacuolar Membrane.
abstract: To better understand plant vacuolar functions and identify new transporters present on the tonoplast, a proteomic work was initiated on Arabidopsis thaliana. A procedure was developed to prepare highly purified vacuoles from protoplasts isolated from Arabidopsis cell cultures, and a proteomics approach was designed to identify the protein components present in both the membrane and soluble fractions of the vacuoles. This procedure allowed the identification of 650 proteins, 2/3 of which copurify with the hydrophobic membrane fraction and 1/3 with the soluble fraction. With regard to function, only 20% of the proteins identified were previously known to be associated with vacuolar activities.
authors.affiliation: Laboratoire d'Etude de la Dynamique des Protéomes Institut de Recherches en Technologies et Sciences pour le Vivant; Commissariat à l'Energie Atomique; Université Joseph Fourier, Grenoble France.
authors.forename: Michel
authors.initials: M
authors.lastname: Jaquinod
authors.forename: Florent
authors.initials: F
authors.lastname: Villiers
authors.forename: Sylvie
authors.initials: S
authors.lastname: Kieffer-Jaquinod
authors.forename: Véronique
authors.initials: V
authors.lastname: Hugouvieux
authors.forename: Christophe
authors.initials: C
authors.lastname: Bruley
authors.forename: Jérôme
authors.initials: J
authors.lastname: Garin
authors.forename: Jacques
authors.initials: J
authors.lastname: Bourguignon
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