title: Emergence of Klebsiella pneumoniae carrying the novel extended-spectrum beta-lactamase gene variants bla(SHV-40), bla(TEM-116) and the class 1 integron-associated bla(GES-7) in Brazil.
abstract: A clinical Klebsiella pneumoniae isolate carrying the extended-spectrum beta-lactamase gene variants bla(SHV-40), bla(TEM-116) and bla(GES-7) was recovered. Cefoxitin and ceftazidime activity was most affected by the presence of these genes and an additional resistance to trimethoprim-sulphamethoxazole was observed. The bla(GES-7) gene was found to be inserted into a class 1 integron. These results show the emergence of novel bla(TEM) and bla(SHV) genes in Brazil. Moreover, the presence of class 1 integrons suggests a great potential for dissemination of bla(GES) genes into diverse nosocomial pathogens. Indeed, the bla(GES-7) gene was originally discovered in Enterobacter cloacae in Greece and, to our knowledge, has not been reported elsewhere.
authors.affiliation: Public Health Laboratory, School of Public Health, University of São Paulo, São Paulo, SP, Brazil. milenadropa@usp.br
authors.forename: M
authors.initials: M
authors.lastname: Dropa
authors.forename: L C
authors.initials: LC
authors.lastname: Balsalobre
authors.forename: N
authors.initials: N
authors.lastname: Lincopan
authors.forename: E M
authors.initials: EM
authors.lastname: Mamizuka
authors.forename: V C
authors.initials: VC
authors.lastname: Cassettari
authors.forename: G R
authors.initials: GR
authors.lastname: Matté
authors.forename: M H
authors.initials: MH
authors.lastname: Matté
mesh_terms: D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D002440:Cefoxitin / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D032023:Integrons; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002440:Cefoxitin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D002442:Ceftazidime; D001618:beta-Lactamases