title: Proteomic evaluation of wound-healing processes in potato (Solanum tuberosum L.) tuber tissue.
abstract: Proteins from potato (Solanum tuberosum L.) tuber slices, related to the wound-healing process, were separated by 2-DE and identified by an MS analysis in MS and MS/MS mode. Slicing triggered differentiation processes that lead to changes in metabolism, activation of defence and cell-wall reinforcement. Proteins related to storage, cell growth and division, cell structure, signal transduction, energy production, disease/defence mechanisms and secondary metabolism were detected. Image analysis of the 2-DE gels revealed a time-dependent change in the complexity of the polypeptide patterns. By microscopic observation the polyalyphatic domain of suberin was clearly visible by D4, indicating that a closing layer (primary suberisation) was formed by then. A PCA of the six sampling dates revealed two time phases, D0-D2 and D4-D8, with a border position between D2 and D4. Moreover, a PCA of differentially expressed proteins indicated the existence of a succession of proteomic events leading to wound-periderm reconstruction. Some late-expressed proteins (D6-D8), including a suberisation-associated anionic peroxidase, have also been identified in the native periderm. Despite this, protein patterns of D8 slices and native periderm were still different, suggesting that the processes of wound-periderm formation are extended in time and not fully equivalent. The information presented in this study gives clues for further work on wound healing-periderm formation processes.
authors.affiliation: Instituto de Tecnologia Química e Biológica (Universidade Nova de Lisboa), Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Chaves
authors.forename: Carla
authors.initials: C
authors.lastname: Pinheiro
authors.forename: Jorge A P
authors.initials: JA
authors.lastname: Paiva
authors.forename: Sébastien
authors.initials: S
authors.lastname: Planchon
authors.forename: Kjell
authors.initials: K
authors.lastname: Sergeant
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: José A
authors.initials: JA
authors.lastname: Graça
authors.forename: Gonçalo
authors.initials: G
authors.lastname: Costa
authors.forename: Ana V
authors.initials: AV
authors.lastname: Coelho
authors.forename: Cândido P Pinto
authors.initials: CP
authors.lastname: Ricardo
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry; D010935:Plant Diseases; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry; D035281:Plant Tubers / Q000166:cytology / Q000378:metabolism*; D025341:Principal Component Analysis; D040901:Proteomics; D011198:Solanum tuberosum / Q000166:cytology / Q000378:metabolism*
chemical_list: D010455:Peptides; D010940:Plant Proteins