title: Enabling customization of non-viral gene delivery systems for individual cell types by surface-induced mineralization.
abstract: Delivering genes to mediate functions of cells is a crucial technology for both basic science and clinical applications. Though numerous non-viral gene delivery systems have been developed, the diversity of mammalian cells poses a great challenge to the material design. Here, we demonstrate that surface-induced mineralization represents a promising approach to systematically customize DNA delivery with respect to the characteristics of cells. We initially examined gene transfer in nine cell types derived from different tissues and organisms by surface-induced DNA-doped calcium carbonate nanocomposites derived from a library of mineral solutions. Subsequently, we correlated gene transfer efficiency with cellular uptake, pH responsiveness of nanocomposites, and phagosomal pH of individual cell types. Based on the correlation, we were able to optimize the DNA delivery to the cell types of interest. Surface-induced mineralization possesses great potential for customizing gene transfer in realizing gene- and cell-based therapy and probing functions of genes.
authors.affiliation: Department of Chemical Engineering, University of Washington, 353 Benson Hall, Box 351750, Seattle, WA 98195, USA.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Sun
authors.forename: Kenny K
authors.initials: KK
authors.lastname: Tran
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*
chemical_list: D004247:DNA
references.citation: Nat Biotechnol. 1997 Jul;15(7):647-52
references.pmid: 9219267
references.citation: Nat Mater. 2004 Aug;3(8):569-74
references.pmid: 15258575
references.citation: Nat Biotechnol. 2008 May;26(5):561-9
references.pmid: 18438401
references.citation: Trends Biotechnol. 2002 Jul;20(7):305-11
references.pmid: 12062976
references.citation: Gene Ther. 2004 Oct;11 Suppl 1:S10-7
references.pmid: 15454952
references.citation: Gene Ther. 2000 Jan;7(2):110-4
references.pmid: 10673715
references.citation: Gene Ther. 2002 Dec;9(24):1647-52
references.pmid: 12457277
references.citation: J Biomed Mater Res. 2001 Oct;57(1):132-9
references.pmid: 11416860
references.citation: Bioconjug Chem. 2002 May-Jun;13(3):621-9
references.pmid: 12009954
references.citation: Am J Clin Pathol. 1974 Jan;61(1):114-7
references.pmid: 4809145
references.citation: Biomaterials. 2004 Aug;25(17):3593-601
references.pmid: 15020133
references.citation: J Biol Chem. 2007 Oct 26;282(43):31422-8
references.pmid: 17724021
references.citation: Nature. 1973 Jun 15;243(5407):420-1
references.pmid: 4743638
references.citation: Gene Ther. 2007 Dec;14(24):1750-3
references.pmid: 17943146
references.citation: Nat Biotechnol. 2000 Jan;18(1):33-7
references.pmid: 10625387
references.citation: Biomaterials. 2009 Mar;30(7):1356-62
references.pmid: 19091401
references.citation: Nat Med. 1996 May;2(5):545-50
references.pmid: 8616713
references.citation: Mol Cell Biol. 1987 May;7(5):2031-4
references.pmid: 3037341
references.citation: Angew Chem Int Ed Engl. 2003 Jul 14;42(27):3153-8
references.pmid: 12866105