Functional characterization of the in vitro folded human y(1) receptor in lipid environment.
We describe the recombinant production of the human Y(1) receptor from inclusion bodies of E. coli cultures. The in vitro refolding was carried out in the presence of lipids from bovine brain extracts. Y(1) receptors in brain lipids compete for cellular receptors in competitive binding experiments.
Schimmer
S
S
Institute of Biochemistry/Biotechnology, Martin Luther University Halle-Wittenberg, Germany.
Lindner
D
D
Schmidt
P
P
Beck-Sickinger
A G
AG
Huster
D
D
Rudolph
R
R
D000818:Animals; D001667:Binding, Competitive; D001923:Brain Chemistry; D002417:Cattle; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism*; D009447:Neuroblastoma; D009478:Neuropeptide Y / Q000378:metabolism; D010759:Phosphorus Isotopes; D017510:Protein Folding; D017476:Receptors, Neuropeptide Y / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014316:Tritium
D008563:Membrane Lipids; D009478:Neuropeptide Y; D010759:Phosphorus Isotopes; D017476:Receptors, Neuropeptide Y; D011994:Recombinant Proteins; D014316:Tritium