Comparative effects of dietary administered transgenic and conventional papaya on selected intestinal parameters in rat models.
Transformation of agricultural crops with novel genes has significantly advanced disease-resistance breeding, including virus resistance through the expression of virus sequences. In this study, the effects of long-term, repeated exposure to transgenic papayas carrying the coat protein gene of Papaya ringspot virus and conventional non-transgenic papaya on the histology and selected biochemical parameters of the intestinal tract were compared. For 3 months, male and female Wistar rats received diets containing transgenic or non-transgenic papaya at twice the equivalent of the average daily consumption of fresh papayas. Gross and macroscopic appearance of intestinal tissues, as well as stomach tissues, was comparable (P < 0.05) as were total intestinal bacterial counts and activities of beta-glucuronidase. Activities of disaccharidases were not affected, neither were those of amylase (P < 0.05). Although significant differences were noted in the activity of Ca(2+) and Na(+)/K(+) ATPase brush border enzymes, no morphological alteration in the integrity of the intestinal mucosa was found. Overall, negligible effects on feed intake, body weight, and fecal output were observed (P < 0.05). Taken together, long-term exposure to diets formulated with transgenic papaya did not result in biologically important unintended effects.
Powell
Melissa
M
Department of Basic Medical Sciences, Biochemistry Section, University of the West Indies, Mona, Jamaica.
Wheatley
Andrew O
AO
Omoruyi
Felix
F
Asemota
Helen N
HN
Williams
Nadia P
NP
Tennant
Paula F
PF
D000681:Amylases / Q000378:metabolism; D000821:Animal Feed / Q000009:adverse effects / Q000821:virology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D029441:Carica / Q000235:genetics / Q000821:virology*; D004186:Disaccharidases / Q000378:metabolism; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D005966:Glucuronidase / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000201:enzymology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000821:virology*; D017800:Potyvirus; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000681:Amylases; D004186:Disaccharidases; D005966:Glucuronidase; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium
J Sci Food Agric. 2014 Mar 30;94(5):1034-8
23983097
Toxicol Pathol. 2002 Jan-Feb;30(1):132-4
11890465
Plant J. 2001 Sep;27(6):503-28
11576435
Dig Dis. 1993 Jul-Oct;11(4-5):298-312
8222310
Transgenic Res. 2007 Jun;16(3):261-80
17436060
Food Chem Toxicol. 2004 Jul;42(7):1047-88
15123382
Int J Toxicol. 2007 Sep-Oct;26(5):389-99
17963126
Mol Plant Pathol. 2008 Jan;9(1):73-83
18705886
Anal Biochem. 1968 Jan;22(1):99-107
5636962
J Natl Cancer Inst. 1976 Aug;57(2):371-5
1003518
Toxicology. 2003 Jun 30;188(2-3):297-307
12767699
Transgenic Res. 1998 May;7(3):157-63
10461390
Biotechnol Annu Rev. 2004;10:85-122
15504704
J Biotechnol. 2004 Feb 5;107(3):193-232
14736458
Toxicol Pathol. 2002 Jan-Feb;30(1):129-31
11892726
Annu Rev Phytopathol. 2007;45:173-202
17408355
Biochem Pharmacol. 1980 Feb 15;29(4):559-64
6245655
Infect Immun. 1974 Dec;10(6):1250-5
4611922
J Gastroenterol Hepatol. 2003 May;18(5):479-97
12702039
Br Poult Sci. 1999 Mar;40(1):88-90
10405041