Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells.
We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells.
Huang
Y H
YH
Department of Gastroenterology, The First Affiliated Hospital, China Medical University, Shenyang , China.
Sun
M J
MJ
Jiang
M
M
Fu
B Y
BY
D000818:Animals; D005934:Glucagon / Q000378:metabolism*; D050416:Glucagon-Secreting Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D010191:Pancreatic Polypeptide / Q000378:metabolism*; D050418:Pancreatic Polypeptide-Secreting Cells / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D010191:Pancreatic Polypeptide; D005934:Glucagon
J Biol Chem. 2006 Sep 29;281(39):29330-6
16873378
Endocr Pathol. 1992 Sep;3(3):134-143
32370451
Endocrinology. 1989 Aug;125(2):801-9
2502376
J Biol Chem. 2000 Dec 29;275(52):41521-7
11024017
Cell Mol Life Sci. 2008 Nov;65(21):3467-80
18810318
Development. 1991 Dec;113(4):1257-65
1811941
Diabetes. 2005 Dec;54(12):3402-9
16306355
C R Acad Hebd Seances Acad Sci D. 1976 Oct 27;283(10):1213-6
827358
Dev Biol. 1992 Oct;153(2):376-85
1356860
J Anat. 1995 Jun;186 ( Pt 3):629-37
7559135
Anat Rec. 1980 Feb;196(2):173-81
6106438
Monogr Pathol. 1980;21:140-55
6163978
J Physiol. 1999 Dec 15;521 Pt 3:717-28
10601501
Diabetes. 2003 Aug;52(8):2066-74
12882924
Gen Comp Endocrinol. 1991 Feb;81(2):187-97
2019394
Anat Embryol (Berl). 1982 Sep;165(1):63-70
6128945
Experientia. 1988 Mar 15;44(3):238-40
3280339
Anat Rec. 1997 Mar;247(3):405-12
9066918
Cell Tissue Res. 1977 Mar 16;178(3):303-6
321125
Diabetes. 2002 Feb;51(2):406-12
11812748
Experientia. 1985 Jan 15;41(1):86-8
3881274
Anat Rec. 1985 Jul;212(3):292-300
3904523
Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8078-83
12048252