Signaling from soybean roots to rhizobium: An ATP-binding cassette-type transporter mediates genistein secretion.
Legume plants have a unique ability to fix atmospheric nitrogen via symbiosis with rhizobia. For the establishment of symbiosis, legume plants secrete signaling molecules such as flavonoids from root tissues, leading to the attraction of rhizobia and the induction of rhizobial nod genes. Genistein and daidzein are found in soybean root exudates and function as signal molecules in soybean-Bradyrhizobium japonicum chemical communication. Although it is more than 20 years since these signal flavonoids were identified, almost nothing has been characterized concerning the membrane transport process of these molecules from soybean roots. To elucidate the transport mechanism we performed membrane transport assays with plasma membrane-enriched vesicles and various inhibitors. As a result, we concluded that an ATP-binding cassette-type transporter is involved in the secretion of genistein from soybean roots. The possible involvement of a pleiotropic drug resistance-type ABC transporter in this secretion is also discussed.
Sugiyama
Akifumi
A
Laboratory of Plant Gene Expression; Research Institute for Sustainable Humanosphere; Kyoto University; Uji, Japan.
Shitan
Nobukazu
N
Yazaki
Kazufumi
K
EMBO J. 1989 Dec 20;8(13):3973-84
2686977
Genome Res. 2001 Jul;11(7):1156-66
11435397
Plant Cell. 2005 Nov;17(11):2922-39
16243904
Curr Opin Plant Biol. 2005 Aug;8(4):346-52
15955723
Int J Cancer. 2004 Jan 1;108(1):146-51
14618629
J Exp Bot. 2002 Apr;53(369):727-35
11886893
EMBO J. 2001 Apr 17;20(8):1875-87
11296221
Nucleic Acids Res. 1992 May 11;20 Suppl:2013-8
1598232
J Biol Chem. 2001 Aug 10;276(32):30231-44
11346655
Mol Cancer Ther. 2003 Jan;2(1):105-12
12533678
DNA Res. 2006 Oct 31;13(5):205-28
17164256
FEBS Lett. 2005 Dec 19;579(30):6791-5
16337204
Curr Opin Plant Biol. 2006 Apr;9(2):110-21
16458572
Plant Cell. 2007 Jan;19(1):131-47
17237354
J Mol Biol. 2004 Oct 8;343(1):249-65
15381434
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7428-32
16593884
EMBO J. 1982;1(8):945-51
6329717
Curr Med Chem. 2007;14(6):689-701
17346156
Plant J. 2004 Jul;39(2):219-36
15225287
Plant J. 2005 Oct;44(2):179-94
16212599
Plant Physiol. 2007 Aug;144(4):2000-8
17556512
Plant Physiol. 2003 Mar;131(3):900-10
12644643
Trends Microbiol. 2006 Apr;14(4):161-8
16520035
J Biol Chem. 1992 Jan 5;267(1):310-8
1730597
Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):751-6
12524452
Science. 1986 Aug 29;233(4767):977-80
3738520
Plant Physiol. 2007 Jun;144(2):588-93
17556522
Science. 2004 Oct 22;306(5696):702-4
15499022