title: Detection and molecular characterization of Giardia isolated  from recreational lake water in Malaysia.
abstract: Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8% of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. limailian@um.edu.my
authors.forename: Yvonne A L
authors.initials: YA
authors.lastname: Lim
authors.forename: Sharmila D
authors.initials: SD
authors.lastname: Ramasame
authors.forename: Mohammed A K
authors.initials: MA
authors.lastname: Mahdy
authors.forename: Wan Yusoff W
authors.initials: WY
authors.lastname: Sulaiman
authors.forename: Huw V
authors.initials: HV
authors.lastname: Smith
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000469:parasitology*; D020459:Genes, rRNA; D005872:Giardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008296:Malaysia; D008969:Molecular Sequence Data; D010274:Parasitology / Q000379:methods; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
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