title: Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins.
abstract: The Tol system is a five-protein assembly parasitized by colicins and bacteriophages that helps stabilize the Gram-negative outer membrane (OM). We show that allosteric signalling through the six-bladed beta-propeller protein TolB is central to Tol function in Escherichia coli and that this is subverted by colicins such as ColE9 to initiate their OM translocation. Protein-protein interactions with the TolB beta-propeller govern two conformational states that are adopted by the distal N-terminal 12 residues of TolB that bind TolA in the inner membrane. ColE9 promotes disorder of this 'TolA box' and recruitment of TolA. In contrast to ColE9, binding of the OM lipoprotein Pal to the same site induces conformational changes that sequester the TolA box to the TolB surface in which it exhibits little or no TolA binding. Our data suggest that Pal is an OFF switch for the Tol assembly, whereas colicins promote an ON state even though mimicking Pal. Comparison of the TolB mechanism to that of vertebrate guanine nucleotide exchange factor RCC1 suggests that allosteric signalling may be more prevalent in beta-propeller proteins than currently realized.
authors.affiliation: Department of Biology, University of York, York, UK.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Bonsor
authors.forename: Oliver
authors.initials: O
authors.lastname: Hecht
authors.forename: Mireille
authors.initials: M
authors.lastname: Vankemmelbeke
authors.forename: Amit
authors.initials: A
authors.lastname: Sharma
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Krachler
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Housden
authors.forename: Katie J
authors.initials: KJ
authors.lastname: Lilly
authors.forename: Richard
authors.initials: R
authors.lastname: James
authors.forename: Geoffrey R
authors.initials: GR
authors.lastname: Moore
authors.forename: Colin
authors.initials: C
authors.lastname: Kleanthous
mesh_terms: D000495:Allosteric Site; D003001:Cloning, Molecular; D003087:Colicins / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D033903:Periplasmic Proteins / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D015398:Signal Transduction
chemical_list: D003087:Colicins; D003432:Cross-Linking Reagents; D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; C062094:tolB protein, E coli
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