title: Arabidopsis thaliana overexpressing glycolate oxidase in chloroplasts: H(2)O(2)-induced changes in primary metabolic pathways.
abstract: Reactive oxygen species (ROS) represent both toxic by-products of aerobic metabolism as well as signaling molecules in processes like growth regulation and defense pathways. The study of signaling and oxidative-damage effects can be separated in plants expressing glycolate oxidase in the plastids (GO plants), where the production of H(2)O(2) in the chloroplasts is inducible and sustained perturbations can reproducibly be provoked by exposing the plants to different ambient conditions. Thus, GO plants represent an ideal non-invasive model to study events related to the perception and responses to H(2)O(2) accumulation. Metabolic profiling of GO plants indicated that under high light a sustained production of H(2)O(2) imposes coordinate changes on central metabolic pathways. The overall metabolic scenario is consistent with decreased carbon assimilation, which results in lower abundance of glycolytic and tricarboxylic acid cycle intermediates, while simultaneously amino acid metabolism routes are specifically modulated. The GO plants, although retarded in growth and flowering, can complete their life cycle indicating that the reconfiguration of the central metabolic pathways is part of a response to survive and thus, to adapt to stress conditions imposed by the accumulation of H(2)O(2) during the light period.
authors.affiliation: Botanisches Institut; Universität zu Köln; Köln Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Fahnenstich
authors.forename: Ulf-Ingo
authors.initials: UI
authors.lastname: Flügge
authors.forename: Verónica G
authors.initials: VG
authors.lastname: Maurino
references.citation: Plant Physiol. 2007 Nov;145(3):640-52
references.pmid: 17885087
references.citation: Plant Physiol. 2006 Jun;141(2):391-6
references.pmid: 16760493
references.citation: Plant Physiol. 2005 Oct;139(2):806-21
references.pmid: 16183842
references.citation: Plant Physiol. 2008 May;147(1):101-14
references.pmid: 18337490
references.citation: Plant Physiol. 2007 Jan;143(1):312-25
references.pmid: 17122072
references.citation: Biochem J. 1991 Jun 15;276 ( Pt 3):643-8
references.pmid: 1648348
references.citation: Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):16113-8
references.pmid: 14671332
references.citation: Plant Physiol. 2006 Jun;141(2):436-45
references.pmid: 16603662
references.citation: Plant Physiol. 1999 Oct;121(2):471-8
references.pmid: 10517838
references.citation: Plant J. 2002 Dec;32(6):891-904
references.pmid: 12492832
references.citation: Plant J. 2003 Apr;34(2):187-203
references.pmid: 12694594
references.citation: Plant Physiol. 2001 Sep;127(1):159-72
references.pmid: 11553744
references.citation: Plant J. 2007 Nov;52(4):640-57
references.pmid: 17877712
references.citation: Plant Physiol. 2008 Oct;148(2):719-29
references.pmid: 18685041