title: Pseudomonas extremaustralis sp. nov., a Poly(3-hydroxybutyrate) producer isolated from an antarctic environment.
abstract: A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3(T) is closely related to Pseudomonas veronii DSM 11331(T), sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, ss-galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37 degrees C but not at 42 degrees C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3(T) therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3(T) has been deposited as DSM 17835(T) and as CIP 109839(T).
authors.affiliation: Dpto. de Qca. Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. nan@qb.fcen.uba.ar
authors.forename: Nancy I
authors.initials: NI
authors.lastname: López
authors.forename: M Julia
authors.initials: MJ
authors.lastname: Pettinari
authors.forename: Erko
authors.initials: E
authors.lastname: Stackebrandt
authors.forename: Paula M
authors.initials: PM
authors.lastname: Tribelli
authors.forename: Markus
authors.initials: M
authors.lastname: Põtter
authors.forename: Alexander
authors.initials: A
authors.lastname: Steinbüchel
authors.forename: Beatriz S
authors.initials: BS
authors.lastname: Méndez
mesh_terms: D000864:Antarctic Regions; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006885:Hydroxybutyrates; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
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