[Research of repairing rabbit knee joint cartilage defect by compound material of fibrin glue and decalcified bone matrix (DBM) and chondrocytes].
OBJECTIVE
To investigate the feasibility and effectivity of using compound material of fibrin glue and DBM as scaffolds for cartilage tissue engineering.


METHODS
Chondrocytes isolated from articular cartilage were seeded into prepared scaffolds, after incubation for 4 weeks in vitro. Chondrocytes and fibrin glue and DBM constructs were implanted in the joint cave of rabbit. The specimens were excised at the 4th, 8th, 12th week, examined grossly analyzed by haematoxylin cosine, toluidine blues staining and type II collagen immunohistochemistry reaction. Wakitani score was counted to evaluate the repairing effect.


RESULTS
Grossly analysis showed some ivory tissue filled the caves after 4 weeks and the caves were full filled with smooth surface after 12 weeks. The microscope showed a good deal of chondrocytes appeared after 8 weeks and more type II collagen than 4 weeks. Twelve weeks later, cartilage lacuna could be observed. The cells arrangement and the amount of type II collagen both showed the same as the natural one.


CONCLUSIONS
Complicated material of fibrin glue and DBM as scaffolds can be used as scaffolds for cartilage tissue engineering.
He
Jie
J
Department of Orthopaedics, the Military General Hospital of Nanjing, the Clinical Medical College of the 2nd Military Surgeon University of Liberation Army, Nanjing 210002, Jiangsu, China.
Yang
Xiang
X
Yue
Peng-ju
PJ
Wang
Guan-yu
GY
Guo
Ting
T
Zhao
Jian-ning
JN
D000818:Animals; D001857:Bone Matrix / Q000502:physiology / Q000637:transplantation*; D002358:Cartilage, Articular / Q000503:physiopathology* / Q000601:surgery; D017690:Cell Transplantation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology / Q000637:transplantation*; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D012038:Regeneration; D014014:Tissue Adhesives / Q000627:therapeutic use*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives