Antinociceptive activity and toxicology of the lectin from Canavalia boliviana seeds in mice.
The aim of the present study was to evaluate the potential antinociceptive and toxicity of Canavalia boliviana lectin (CboL) using different methods in mice. The role of carbohydrate-binding sites was also investigated. CboL given to mice daily for 14 days at doses of 5 mg/kg did not cause any observable toxicity. CboL (1, 5, and 10 mg/kg) administered to mice intravenously inhibited abdominal constrictions induced by acetic acid and the two phases of the formalin test. In the hot plate and tail immersion tests, the same treatment of CboL induced significant increase in the latency period. In the hot plate test, the effect of CboL (5 mg/kg) was reversed by naloxone (1 mg/kg), indicating the involvement of the opioid system. In the open-field and rota-rod tests, the CboL treatment did not alter animals' motor function. These results show that CboL presents antinociceptive effects of both central and peripheral origin, involving the participation of the opioid system via lectin domain.
Figueiredo
Jozi Godoy
JG
Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60451-970, Fortaleza, CE, Brazil.
da Silveira Bitencourt
Flávio
F
Beserra
Ingrid Gonçalves
IG
Teixeira
Cícero Silvano
CS
Luz
Patrícia Bastos
PB
Bezerra
Eduardo Henrique Salviano
EH
Mota
Mário Rogério Lima
MR
Assreuy
Ana Maria Sampaio
AM
de Queiroz Cunha
Fernando
F
Cavada
Benildo Sousa
BS
de Alencar
Nylane Maria Nunes
NM
D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D032449:Canavalia / Q000737:chemistry*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D037121:Plant Lectins / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D012639:Seeds
D000700:Analgesics; D037121:Plant Lectins; C039268:lectin, Canavalia
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