title: Diffusive movement of processive kinesin-1 on microtubules.
abstract: The processive motor kinesin-1 moves unidirectionally toward the plus end of microtubules. This process can be visualized by total internal reflection fluorescence microscopy of kinesin bound to a carboxylated quantum dot (Qdot), which acts both as cargo and label. Surprisingly, when kinesin is bound to an anti-HIS Qdot, it shows diffusive movement on microtubules, which decreased in favor of processive runs with increasing salt concentration. This observation implies that kinesin movement on microtubules is governed by its conformation, as it is well established that kinesin undergoes a salt-dependent transition from a folded (inactive) to an extended (active) molecule. A truncated kinesin lacking the last 75 amino acids (kinesin-Delta C) showed both processive and diffusive movement on microtubules. The extent of each behavior depends on the relative amounts of ADP and ATP, with purely diffusive movement occurring in ADP alone. Taken together, these data imply that folded kinesin.ADP can exist in a state that diffuses along the microtubule lattice without expending energy. This mechanism may facilitate the ability of kinesin to pick up cargo, and/or allow the kinesin/cargo complex to stay bound after encountering obstacles.
authors.affiliation: Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Hailong
authors.initials: H
authors.lastname: Lu
authors.forename: M Yusuf
authors.initials: MY
authors.lastname: Ali
authors.forename: Carol S
authors.initials: CS
authors.lastname: Bookwalter
authors.forename: David M
authors.initials: DM
authors.lastname: Warshaw
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Trybus
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004058:Diffusion; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D009068:Movement; D011487:Protein Conformation; D017510:Protein Folding; D021381:Protein Transport; D045663:Quantum Dots
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C475469:Kif5b protein, mouse; D016547:Kinesins
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