SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages.
This work was designed to study the role of surfactant protein D in the regulation of NO synthesis by "non-alveolar" microphages. We evaluated whether the effects of surfactant protein D depend on the phenotype of macrophages. In the absence of surfactant protein D, the LPS-induced iNOS response was shown to decrease in macrophages of native and proinflammatory phenotypes by 30%, and in macrophages of the antiinflammatory phenotype (by 63%). Under the influence of lipopolysaccharide in high doses (500 ng/ml), NO(2)*- production by mouse macrophages without surfactant protein D was reduced in native cells (by 25%), but increased in proinflammatory (by 40%) and antiinflammatory phenotypes (by 12% compared to mouse macrophages with surfactant protein D). Our results suggest that surfactant protein D is involved in the immune response in the whole organism, but not only in the lungs. The effect of surfactant protein D depends on the phenotype of macrophages.
Atochina-Vasserman
E N
EN
Russian Academy of Medical Sciences, Moscow, Russia.
Abramova
E V
EV
Tomer
Y
Y
Scott
P
P
Nazarov
V A
VA
Kruglov
S V
SV
Beers
M F
MF
Gow
A J
AJ
Malyshev
I Yu
IY
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology / Q000503:physiopathology*; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D016207:Cytokines; D008070:Lipopolysaccharides; D009573:Nitrites; D037663:Pulmonary Surfactant-Associated Protein D; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse
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