title: Identification of odor impact compounds of Tagetes minuta L. essential oil: comparison of two GC-olfactometry methods.
abstract: Odor impact compounds of Tagetes minuta L. essential oil were studied by gas chromatography (GC)-olfactometry using aroma extract dilution analysis (AEDA) and vocabulary-intensity-duration of elementary odors by sniffing (VIDEO-Sniff). AEDA was conducted by direct injection and revealed the presence of 43 odorant zones. Highest flavor dilution (FD) values were obtained for ethyl 2-methylpropanoate, ethyl 3-methylbutanoate, (E)-ocimenone, two tentatively identified thiols, and two yet unknown compounds. VIDEO-Sniff was realized by dynamic headspace sampling (D-HS) combined with 8W-GC-olfactometry where eight sniffers simultaneously detect volatile compounds obtained from a single chromatographic separation and revealed the presence of 42 odorant zones. Odorant trace compounds detected by GC-O that were present in quantities inferior to the GC-qMS system's detection limit and those subject to coelutions were identified by GC x GC-time-of-flight mass spectrometry (TOFMS). A total amount of 37 odorant components could be identified by VIDEO-Sniff, and the strong influence of the fruity notes of numerous esters stood out. Highest olfactory signals were obtained for ethyl 2-methylpropanoate, ethyl 2- and 3-methylbutanoate, and oct-1-en-3-one. Both methods hence come to the conclusion that ethyl 2-methylpropanoate and ethyl 2- and 3-methylbutanoate are among the main odorants in Tagetes minuta L. essential oil. Differences, advantages, and drawbacks of both GC-O methods are discussed.
authors.affiliation: LCMBA UMR CNRS 6001, Universite de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Katharina
authors.initials: K
authors.lastname: Breme
authors.forename: Pascal
authors.initials: P
authors.lastname: Tournayre
authors.forename: Xavier
authors.initials: X
authors.lastname: Fernandez
authors.forename: Uwe J
authors.initials: UJ
authors.lastname: Meierhenrich
authors.forename: Hugues
authors.initials: H
authors.lastname: Brevard
authors.forename: Daniel
authors.initials: D
authors.lastname: Joulain
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Berdagué
mesh_terms: D002087:Butyrates / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D009812:Odorants / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry; D011422:Propionates / Q000032:analysis; D012903:Smell; D031686:Tagetes / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D002087:Butyrates; D009822:Oils, Volatile; D010938:Plant Oils; D011422:Propionates; D055549:Volatile Organic Compounds; C546702:ethyl 2-methylpropanoate