title: Oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in T cells, macrophages and dendritic cells.
abstract: BACKGROUND
  The human immunodeficiency virus (HIV) can integrate into T cells, macrophages and dendritic cells resulting in a latent infection. Reports have also demonstrated that various microbial and host cell factors can trigger HIV reactivation leading to HIV recrudescence, potentially undermining highly active antiretroviral therapies.
  
  
  METHODS
  This study evaluated the capacity of oral bacteria associated with chronic periodontal infections to stimulate HIV promoter activation in various cell models of HIV latency.
  
  
  RESULTS
  T cells (1G5) challenged with oral bacteria demonstrated a dose-response of HIV promoter activation with a subset of the bacteria, as well as kinetics that were generally similar irrespective of the stimuli. Direct bacterial challenge of the T cells resulted in increased activation of approximately 1.5- to 7-fold over controls. Challenge of macrophages (BF24) indicated different kinetics for individual bacteria and resulted in consistent increases in promoter activation of five fold to six fold over basal levels for all bacteria except Streptococcus mutans. Dendritic cells showed increases in HIV reactivation of 7- to 34-fold specific for individual species of bacteria.
  
  
  CONCLUSIONS
  These results suggested that oral bacteria have the capability to reactivate HIV from latently infected cells, showing a relationship of mature dendritic cells > immature dendritic cells > macrophages > or = T cells. Expression of various pattern recognition receptors on these various cell types may provide insight into the primary receptors/signaling pathways used for reactivation by the bacteria.
authors.affiliation: Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY 40536, USA. chuan2@uky.edu
authors.forename: C B
authors.initials: CB
authors.lastname: Huang
authors.forename: K A
authors.initials: KA
authors.lastname: Emerson
authors.forename: O A
authors.initials: OA
authors.lastname: Gonzalez
authors.forename: J L
authors.initials: JL
authors.lastname: Ebersole
mesh_terms: D016924:Actinomyces viscosus / Q000502:physiology; D016976:Aggregatibacter actinomycetemcomitans / Q000502:physiology; D001439:Bacteroides / Q000502:physiology; D044883:Campylobacter rectus / Q000502:physiology; D002460:Cell Line; D055113:Chronic Periodontitis / Q000382:microbiology*; D003713:Dendritic Cells / Q000821:virology*; D016967:Fusobacterium nucleatum / Q000502:physiology; D006090:Gram-Negative Bacteria / Q000502:physiology*; D006094:Gram-Positive Bacteria / Q000502:physiology*; D015658:HIV Infections / Q000821:virology; D016325:HIV Long Terminal Repeat / Q000502:physiology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000502:physiology; D008264:Macrophages / Q000821:virology*; D009055:Mouth / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000502:physiology; D018713:Prevotella intermedia / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000502:physiology; D013295:Streptococcus mutans / Q000502:physiology; D013601:T-Lymphocytes / Q000821:virology*; D014162:Transfection; D014775:Virus Activation / Q000502:physiology*; D017735:Virus Latency / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides
references.citation: Nat Med. 2006 Dec;12(12):1365-71
references.pmid: 17115046
references.citation: J Surg Res. 1997 Nov;73(1):85-9
references.pmid: 9441798
references.citation: Blood. 2004 Mar 15;103(6):2170-9
references.pmid: 14630806
references.citation: Dent Clin North Am. 2005 Jul;49(3):491-516, v
references.pmid: 15978238
references.citation: Eur J Immunol. 2002 Jan;32(1):59-66
references.pmid: 11754004
references.citation: J Infect Dis. 2000 Jun;181(6):2041-4
references.pmid: 10837189
references.citation: AIDS. 2008 Sep;22 Suppl 3:S7-12
references.pmid: 18845925
references.citation: Nat Med. 2001 May;7(5):530-2
references.pmid: 11329047
references.citation: Mol Cell Biol. 2001 Jan;21(2):438-48
references.pmid: 11134332
references.citation: J Virol. 2005 Jan;79(1):17-27
references.pmid: 15596797
references.citation: J Leukoc Biol. 2006 Nov;80(5):1127-35
references.pmid: 16923921
references.citation: J Biol Chem. 2007 Feb 9;282(6):3594-604
references.pmid: 17150965
references.citation: Inflamm Allergy Drug Targets. 2008 Dec;7(4):211-6
references.pmid: 19075786
references.citation: Immunology. 2008 Aug;124(4):445-52
references.pmid: 18540962
references.citation: Infect Immun. 2005 Feb;73(2):935-43
references.pmid: 15664935
references.citation: Int J Mol Med. 2000 Jun;5(6):615-8
references.pmid: 10812011
references.citation: Cell Microbiol. 2007 Aug;9(8):1891-901
references.pmid: 17521328
references.citation: Curr HIV Res. 2008 Sep;6(5):388-400
references.pmid: 18855649
references.citation: Immunology. 2008 Oct;125(2):145-53
references.pmid: 18798918
references.citation: Infect Immun. 1999 Sep;67(9):4340-5
references.pmid: 10456872
references.citation: Retrovirology. 2006 Nov 03;3:77
references.pmid: 17083721
references.citation: Nat Immunol. 2001 Apr;2(4):338-45
references.pmid: 11276205
references.citation: J Periodontol. 1988 Oct;59(10):684-7
references.pmid: 3054046
references.citation: Oral Microbiol Immunol. 1989 Dec;4(4):183-92
references.pmid: 2640312
references.citation: Periodontol 2000. 2007;43:278-93
references.pmid: 17214845
references.citation: Oral Dis. 2009 Jul;15(5):313-27
references.pmid: 19364391
references.citation: Infect Immun. 1980 Dec;30(3):862-73
references.pmid: 7228391
references.citation: Infect Immun. 1988 Aug;56(8):2028-35
references.pmid: 3260893
references.citation: J Leukoc Biol. 2003 Nov;74(5):726-35
references.pmid: 12960226
references.citation: Ann Rheum Dis. 2008 Dec;67 Suppl 3:iii44-9
references.pmid: 19022813
references.citation: J Cell Mol Med. 2002 Jul-Sep;6(3):415-25
references.pmid: 12417058
references.citation: J Leukoc Biol. 1998 Jul;64(1):25-32
references.pmid: 9665271
references.citation: Int Rev Immunol. 2008;27(4):255-81
references.pmid: 18574739
references.citation: Immunity. 2008 Nov 14;29(5):795-806
references.pmid: 18951047
references.citation: J Cell Mol Med. 2002 Apr-Jun;6(2):223-34
references.pmid: 12169207
references.citation: Infect Immun. 1984 Jan;43(1):166-77
references.pmid: 6360892
references.citation: J Infect Dis. 1999 May;179 Suppl 3:S427-30
references.pmid: 10099112
references.citation: J Clin Virol. 2007 Apr;38(4):348-52
references.pmid: 17339129
references.citation: Eur J Immunol. 2003 May;33(5):1399-408
references.pmid: 12731067
references.citation: J Leukoc Biol. 2003 Dec;74(6):1045-55
references.pmid: 12960272
references.citation: Infect Immun. 2001 Sep;69(9):5375-84
references.pmid: 11500407
references.citation: Immunity. 2008 Jul 18;29(1):12-20
references.pmid: 18631453
references.citation: Ann Diagn Pathol. 1998 Jun;2(3):181-94
references.pmid: 9845738
references.citation: Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10615-9
references.pmid: 7938001
references.citation: Bull Mem Acad R Med Belg. 2004;159(Pt 2):176-89
references.pmid: 15615091
references.citation: J Biol Chem. 2004 Aug 20;279(34):36072-82
references.pmid: 15199068
references.citation: J Periodontal Res. 1983 Sep;18(5):541-57
references.pmid: 6227738
references.citation: Am J Physiol Gastrointest Liver Physiol. 2006 Apr;290(4):G839-45
references.pmid: 16293657