[Discrimination of anticancer agent action loci at G(2) and M phases by flow cytometry and confocal microscopic imaging].
This study was purposed to evaluate a method to discriminate the action loci of anticancer agents in G(2) and M phases of cell cycle. The meta-amsacrine (m-AMSA) and vinblastine (VBL), already known as G(2) and M phase arrest agent respectively, were used to induce the arrest of MOLT-4 cells at G(2) and M phases, the change of DNA content was detected by flow cytometry, the morphology of arrested cells was observed by confocal microscopy so as to find the arrest efficacy difference of 2 anticancer agents. As a result, the flow cytometric detection showed that the arrested MOLT-4 cells displayed the raise of peaks in G(2) and M phases, but flow cytometric detection alone can not discriminate the difference between them. The observation with confocal microscopy showed that the MOLT-4 cells arrested by m-AMSA displayed the morphologic features in G(2) phase, while the MOLT-4 cells arrested by VBL displayed the morphologic features in M phase. This observation with confocal microscopy is helpful to discriminate the difference between them. In conclusion, the combination of flow cytometry with confocal microscopy is one of the effective methods to discriminate the kind of G(2) or M phase arresting agent of anticancer drugs.
Zhong
Yi-Sheng
YS
Department of Hepatic Surgery, Xiamen Municipal Hospital of Chinese Traditional Medicine, Xiamen 361009, Fujian Province, China.
Pan
Chang-Chuan
CC
Jin
Chang-Nan
CN
Lin
Jian-Jun
JJ
Xiong
Gong-Peng
GP
Zhang
Jian-Xi
JX
Gong
Jian-Pin
JP
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D018613:Microscopy, Confocal; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents