title: An electrochemical glucose biosensor exploiting a polyaniline grafted multiwalled carbon nanotube/perfluorosulfonate ionomer-silica nanocomposite.
abstract: A glucose biosensor was fabricated with loading of glucose oxidase (GOx) into a new organic-inorganic hybrid nanocomposite. The preparation involves formation of silica network into a Nafion (perfluorosulfonate ionomer) and subsequent loading of polyaniline grafted multiwalled carbon nanotubes (MWNT-g-PANI) onto Nafion-silica nanocomposite. Field emission scanning electron microscopy (FE-SEM) of Nafion-silica/MWNT-g-PANI composite reveals the presence of spherical silica particles (sizes in the range 250 nm-1 microm) and tubular MWNT-g-PANI particles. Chronoamperometry and cyclic voltammetry were used to evaluate the performance of biosensor towards glucose. The Nafion-silica/MWCNT-g-PANI/GOx biosensor exhibited a linear response to glucose in the concentration range of 1-10 mm with a correlation coefficient of 0.9972, good sensitivity (5.01 microA/mm), a low response time (approximately 6s), repeatability (R.S.D value of 2.2%) and along-term stability. The presence of silica network within Nafion and MWNT-g-PANI synergistically contributes to the performance of the biosensor towards the electrochemical detection of glucose.
authors.affiliation: Department of Chemistry Graduate School, Kyungpook National University, Daegu, South Korea. algopal_99@yahoo.com
authors.forename: Anantha I
authors.initials: AI
authors.lastname: Gopalan
authors.forename: Kwang P
authors.initials: KP
authors.lastname: Lee
authors.forename: Dhanusuraman
authors.initials: D
authors.lastname: Ragupathy
authors.forename: Se H
authors.initials: SH
authors.lastname: Lee
authors.forename: Jong W
authors.initials: JW
authors.lastname: Lee
mesh_terms: D000180:Acrylic Resins; D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; D037742:Nanotubes, Carbon; C015897:glass ionomer; C416807:polyaniline; C040402:perfluorosulfonic acid; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D005947:Glucose