Changes in Phospholipid Composition Studied by HPLC and Electric Properties of Liver Cell Membrane of Ethanol-Poisoned Rats.
Ethanol introduced into the organism undergoes rapid metabolism to acetaldehyde and then to acetic acid. The process is accompanied by formation of reactive oxygen species (ROS), which damage mainly lipids of membrane cells. The effects of ROS can be neutralized by administering preparations with antioxidant properties. The natural preparations of this kind are teas.This paper reports data on the effect of green and black tea on the surface charge density, content of phospholipids, and level of lipid peroxidation products of liver cell membrane of rats chronically intoxicated with ethanol. Surface charge density of liver cells was measured by the electrophoresis method, whereas qualitative phospholipid composition was determined by the HPLC method.Ethanol administration caused an increase in the amount of all phospholipids, in surface charge density as well as in lipid peroxidation products. Ingestion of green and black tea with ethanol partially prevented these ethanol-induced changes, and the action of green tea was stronger than that of black tea.
Szachowicz-Petelska
Barbara
B
Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4,15-443, Białystok, Poland.
Dobrzyńska
Izabela
I
Skrzydlewska
Elżbieta
E
Figaszewski
Zbigniew A
ZA
Biochem J. 1995 Jan 15;305 ( Pt 2):643-9
7832784
Prev Med. 2005 Jun;40(6):910-8
15850895
Alcohol. 1990 Jan-Feb;7(1):49-59
2178630
Clin Chim Acta. 1997 Jan 3;257(1):59-84
9028626
Toxicology. 2000 Aug 7;148(2-3):93-101
10962127
J Nutr. 2001 Sep;131(9):2248-51
11533262
Alcohol Clin Exp Res. 1985 Mar-Apr;9(2):164-80
2988363
J Chromatogr B Biomed Sci Appl. 1997 May 9;692(2):311-7
9188819
Biochemistry. 1993 Apr 13;32(14):3714-21
8466911
Methods Enzymol. 1978;52:302-10
672633
Mol Cell Biochem. 2000 Mar;206(1-2):125-32
10839202
Mol Cell Biochem. 2002 Sep;238(1-2):41-7
12349908
Biomed Pharmacother. 1997;51(8):305-10
9436520
Free Radic Biol Med. 1996;20(7):933-56
8743980
Biochem J. 1970 Mar;116(5):833-42
4315049
Alcohol Clin Exp Res. 1998 Dec;22(9):2118-24
9884160
NMR Biomed. 1999 Nov;12(7):413-39
10654290
J Nutr. 2003 Oct;133(10):3275S-84S
14519826
Clin Chim Acta. 1995 Mar 31;235(2):179-88
7554272
Chem Biol Interact. 2005 Sep 10;156(1):41-53
16098958
J Stud Alcohol. 2006 Jul;67(4):510-8
16736070
J Agric Food Chem. 2000 Dec;48(12):5834-41
11312761
Addict Biol. 2002 Jul;7(3):307-14
12126490
Drug Alcohol Depend. 1990 Feb;25(1):67-71
2323311
Anal Biochem. 2000 Mar 15;279(2):164-9
10706785
Pharmacol Rev. 2000 Dec;52(4):673-751
11121513
Free Radic Biol Med. 1988;5(4):237-46
3075951
Biochem Biophys Res Commun. 1998 Mar 27;244(3):903-7
9535765
Alcohol Clin Exp Res. 1998 May;22(S3 Pt 1):150S-155S
9622394
Alcohol. 1998 Nov;16(4):311-6
9818983