title: Hsp90 is regulated by a switch point in the C-terminal domain.
abstract: Heat shock protein 90 (Hsp90) is an abundant, dimeric ATP-dependent molecular chaperone, and ATPase activity is essential for its in vivo functions. S-nitrosylation of a residue located in the carboxy-terminal domain has been shown to affect Hsp90 activity in vivo. To understand how variation of a specific amino acid far away from the amino-terminal ATP-binding site regulates Hsp90 functions, we mutated the corresponding residue and analysed yeast and human Hsp90 variants both in vivo and in vitro. Here, we show that this residue is a conserved, strong regulator of Hsp90 functions, including ATP hydrolysis and chaperone activity. Unexpectedly, the variants alter both the C-terminal and N-terminal association properties of Hsp90, and shift its conformational equilibrium within the ATPase cycle. Thus, S-nitrosylation of this residue allows the fast and efficient fine regulation of Hsp90.
authors.affiliation: Center for Integrated Protein Science, Department of Chemistry, Technische Universität München, Garching, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Retzlaff
authors.forename: Michael
authors.initials: M
authors.lastname: Stahl
authors.forename: H Christian
authors.initials: HC
authors.lastname: Eberl
authors.forename: Stephan
authors.initials: S
authors.lastname: Lagleder
authors.forename: Jürgen
authors.initials: J
authors.lastname: Beck
authors.forename: Horst
authors.initials: H
authors.lastname: Kessler
authors.forename: Johannes
authors.initials: J
authors.lastname: Buchner
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: C074510:HSP82 protein, S cerevisiae; D018841:HSP90 Heat-Shock Proteins; C533585:HSP90AA2P protein, human; C493165:HSP90AB1 protein, human; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases
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