Liquid chromatographic analysis of Hg(II) binding by thiol-rich peptides using both UV-vis and electrochemical detection.
An existing method for HPLC determination of thiol-containing peptides has been successfully adapted to the analysis of mixtures of glutathione (GSH) and some related peptides with their Hg(II) complexes as a first approach to the study of phytochelatin extracts. The separation was achieved in a C18 column with a mobile phase of 0.1% trifluoroacetic acid (TFA) and 0.1% TFA/acetonitrile. Non-derivative UV-vis detection at 202 nm used in the original method has been complemented with amperometric detection at 1.2 V on glassy carbon electrode. Two different Hg(II)-GSH complexes were observed by both detection modes and confirmed by mass spectrometry.
Dago
Angela
A
Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès, 1-11, E-08028 Barcelona, Spain.
González-García
Olga
O
Ariño
Cristina
C
Díaz-Cruz
José Manuel
JM
Esteban
Miquel
M
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D008628:Mercury / Q000737:chemistry*; D054811:Phytochelatins / Q000737:chemistry / Q000302:isolation & purification*; D013053:Spectrophotometry
D054811:Phytochelatins; D008628:Mercury