title: [Autocrine effect of vascular endothelial growth factor on the proliferation of HaCaT cells].
abstract: OBJECTIVE
  To determine the autocrine effect of vascular endothelial growth factor (VEGF) on epidermal keratinocytes HaCaT cells.
  
  
  METHODS
  Cultured HaCaT cells were treated with various concentrations of VEGF(165) (0,1,5,10,25,50,100 ng/ml) or Avastin (0,0.063,0.125,0.25,0.50,1.0,2.0 mg/ml) in vitro. HaCaT cell proliferation was determined by MTT assay and the cell migration was measured by migration assay. The effect of VEGF(165) (10 ng/ml) on phosphorylation of ERK1/2 was detected in HaCaT cells pretreated or not pretreated with Avastin (0.5 mg/ml).
  
  
  RESULTS
  VEGF enhanced the proliferation and migration of HaCaT cells in a dose-dependent manner, while Avastin inhibited the effects of VEGF also in a dose-dependent manner. VEGF(165) (10 ng/ml) induced the phosphorylation of ERK1/2 in HaCaT cells,but which was blocked by Avastin (0.5 mg/ml).
  
  
  CONCLUSIONS
  VEGF enhanced the proliferation and migration of HaCaT cells in a dose-dependent manner, while Avastin inhibited the effects of VEGF also in a dose-dependent manner. VEGF(165) (10 ng/ml) induced the phosphorylation of ERK1/2 in HaCaT cells,but which was blocked by Avastin (0.5 mg/ml).
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: YANG
authors.forename: Xiao-yong
authors.initials: XY
authors.lastname: MAN
authors.forename: Sui-qing
authors.initials: SQ
authors.lastname: CAI
authors.forename: Chun-ming
authors.initials: CM
authors.lastname: LI
authors.forename: Jiong
authors.initials: J
authors.lastname: ZHOU
authors.forename: Min
authors.initials: M
authors.lastname: ZHENG
mesh_terms: D019898:Autocrine Communication; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D012867:Skin / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D042461:Vascular Endothelial Growth Factor A