In Vitro and In Vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella.
Punica granatum is commonly used in Korea as a traditional medicine for the treatment of pathogenic bacteria. In this study, we investigated the in vitro and in vivo antimicrobial activity of P. granatum peel EtOH extract (PGPE) against 16 strains of Salmonella. The minimal inhibitory concentrations of PGPE were in the range of 62.5-1000 x03BCg mL(-1). In addition, the in vivo antibacterial activity of the PGPE extract was examined in a S. typhimurium infection mouse model. Mice were initially infected with S. typhimurium and then with PGPE. The extract was found to have significant effects on mortality and the numbers of viable S. typhimurium recovered from feces. Although clinical signs and histological damage were rarely observed in the treated mice, the untreated controls showed signs of lethargy and histological damage in the liver and spleen. Taken together, the results of this study indicate that PGPE has the potential to provide an effective treatment for salmonellosis.
Choi
Jang-Gi
JG
College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.
Kang
Ok-Hwa
OH
Lee
Young-Seob
YS
Chae
Hee-Sung
HS
Oh
You-Chang
YC
Brice
Obiang-Obounou
OO
Kim
Min-San
MS
Sohn
Dong-Hwan
DH
Kim
Hun-Soo
HS
Park
Hyun
H
Shin
Dong-Won
DW
Rho
Jung-Rae
JR
Kwon
Dong-Yeul
DY
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