title: Antinociceptive and antiedematogenic activities of andrographolide isolated from Andrographis paniculata in animal models.
abstract: The current study was performed to evaluate the antinociceptive and antiedematogenic properties of andrographolide isolated from the leaves of Andrographis paniculata using two animal models. Antinociceptive activity was evaluated using the acetic acid- induced writhing and the hot-plate tests, while antiedematogenic activity was measured using the carrageenan-induced paw edema test. Subcutaneous (s.c.) administration of andrographolide (10, 25, and 50 mg/kg) did not affect the motor coordination of the experimental animals but produced significant (p < .05) antinociceptive activity when assessed using both tests. However, 2 mg/kg naloxone failed to affect the 25 mg/kg andrographolide activity in both tests, indicating that the activity was modulated via nonopioid mechanisms. Furthermore, andrographolide showed significant (p < .05) antiedematogenic activity. In conclusion, the results obtained suggest that andrographolide has antinociceptive and antiedematogenic activities; it may be useful for treating pain and inflammation once human studies are conducted.
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. mrs@medic.upm.edu.my
authors.forename: M R
authors.initials: MR
authors.lastname: Sulaiman
authors.forename: Z A
authors.initials: ZA
authors.lastname: Zakaria
authors.forename: A
authors.initials: A
authors.lastname: Abdul Rahman
authors.forename: A S
authors.initials: AS
authors.lastname: Mohamad
authors.forename: M N
authors.initials: MN
authors.lastname: Desa
authors.forename: J
authors.initials: J
authors.lastname: Stanslas
authors.forename: S
authors.initials: S
authors.lastname: Moin
authors.forename: D A
authors.initials: DA
authors.lastname: Israf
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D004195:Disease Models, Animal; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000700:Analgesics; D004224:Diterpenes; C030419:andrographolide