Catalytically incompetent by design.
Sondermann and colleagues have characterized FimX, a protein with degenerate GGDEF and EAL domains. The study confirms the expected domain folds lacking conserved catalytic residues for c-di-GMP synthesis/degradation, and also defines domain arrangements, providing insight to regulatory mechanisms.
Gao
Rong
R
Stock
Ann M
AM
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D018360:Crystallography, X-Ray; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D001426:Bacterial Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP
Structure. 2009 Aug 12;17(8):1104-16
19679088
Nature. 2009 Jun 18;459(7249):1015-8
19536266
Structure. 2007 Aug;15(8):915-27
17697997
J Bacteriol. 2005 Jul;187(14):4774-81
15995192
BMC Microbiol. 2005 Jun 14;5:35
15955239
J Biol Chem. 2005 Sep 2;280(35):30829-37
15994307
Annu Rev Microbiol. 2009;63:133-54
19575571
Nat Rev Microbiol. 2009 Apr;7(4):263-73
19287449
J Biol Chem. 2009 May 8;284(19):13174-84
19244251
J Bacteriol. 2009 Aug;191(15):4722-31
19376848
Genes Dev. 2009 Jan 1;23(1):93-104
19136627
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3461-6
19218451