Alternative splicing generates a novel FADS2 alternative transcript in baboons.
The mammalian fatty acid desaturase 2 (FADS2) gene codes for catalytic activity considered to be the rate limited step in long chain polyunsaturated fatty acid (LCPUFA) synthesis. FADS2 catalyzes 6-desaturation in at least five substrates and 8-desaturation in at least two substrates. However, the molecular mechanisms that regulate FADS2-mediated desaturation remain ill-defined. We report here characterization of an alternative transcript (AT1) of primate FADS2 and compare its expression to that of the classical transcript in 12 tissues of a 12 week old neonate baboon, and in human SK-N-SH neuroblastoma (NB) cells. RT-PCR analysis indicates relatively greater abundance of classical transcript than AT1 in all tissues. However, AT1 expression is highly variable, showing greater expression in liver, retina, occipital lobe, hippocampus, spleen, and ovary, than in other tissues, whereas classical transcript displayed little variability. These data suggest that FADS2 AT1 is a candidate for regulation of LCPUFA synthesis.
Park
Woo Jung
WJ
Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.
Reardon
Holly T
HT
Tyburczy
Cynthia
C
Kothapalli
Kumar S D
KS
Brenna
J Thomas
JT
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D044943:Fatty Acid Desaturases / Q000235:genetics*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D010215:Papio / Q000235:genetics*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology
D012333:RNA, Messenger; D044943:Fatty Acid Desaturases
Pediatr Res. 2007 May;61(5 Pt 1):537-45
17413857
Gene. 2009 Oct 1;446(1):28-34
19573581
Bioinformatics. 2004 Mar 1;20(4):547-56
14990451
J Lipid Res. 2009 Jun;50(6):1195-202
19202133
Biochem J. 2002 May 15;364(Pt 1):49-55
11988075
Annu Rev Nutr. 2004;24:345-76
15189125
Biochem Soc Trans. 2004 Feb;32(Pt 1):86-7
14748719
Eur J Biochem. 1996 Mar 15;236(3):820-6
8665900
Genomics. 2000 Jun 1;66(2):175-83
10860662
Plant Physiol. 2007 May;144(1):455-67
17384161