Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis.
The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends generated a complete genome of 6,586 nucleotides. Phylogenetic analysis demonstrated that this virus, named astrovirus VA1 (AstV-VA1), is highly divergent from all previously described astroviruses. Based on RT-PCR, specimens from multiple patients in this outbreak were unequivocally positive for Ast-VA1.
Finkbeiner
Stacy R
SR
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Li
Yan
Y
Ruone
Susan
S
Conrardy
Christina
C
Gregoricus
Nicole
N
Toney
Denise
D
Virgin
Herbert W
HW
Anderson
Larry J
LJ
Vinjé
Jan
J
Wang
David
D
Tong
Suxiang
S
D000328:Adult; D000818:Animals; D019350:Astroviridae Infections / Q000453:epidemiology / Q000821:virology; D002656:Child Day Care Centers; D002675:Child, Preschool; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity
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