title: Optimization of 2-ethylhexyl palmitate production using lipozyme RM IM as catalyst in a solvent-free system.
abstract: This work reports the application of a lipase in the 2-ethylhexyl palmitate esterification in a solvent-free system with an immobilized lipase (Lipozyme RM IM). A sequential strategy was used applying two experimental designs to optimize the 2-ethylhexyl palmitate production. An empirical model was then built so as to assess the effects of process variables on the reaction conversion. Afterwards, the operating conditions that optimized 2-ethylhexyl palmitate production were established as being acid/alcohol molar ratio 1:3, temperature of 70 degrees C, stirring rate of 150 rpm, 10 wt.% of enzyme, leading to a reaction conversion as high as 95%. From this point, a kinetic study was carried out evaluating the effect of acid:alcohol molar ratio, the enzyme concentration and the temperature on product conversion. The results obtained in this step permit to verify that an excess of alcohol (acid to alcohol molar ratio of 1:6), relatively low enzyme concentration (10 wt.%) and temperature of 70 degrees C, led to conversions next to 100%.
authors.affiliation: Escola de Química-EQ/UFRJ, CT, Bloco E, Rio de Janeiro, 21945-900, RJ, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Richetti
authors.forename: Selma G F
authors.initials: SG
authors.lastname: Leite
authors.forename: Octávio A C
authors.initials: OA
authors.lastname: Antunes
authors.forename: Andrea L F
authors.initials: AL
authors.lastname: de Souza
authors.forename: Lindomar A
authors.initials: LA
authors.lastname: Lerin
authors.forename: Rogério M
authors.initials: RM
authors.lastname: Dallago
authors.forename: Natalia
authors.initials: N
authors.lastname: Paroul
authors.forename: Marco
authors.initials: M
authors.lastname: Di Luccio
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D008049:Lipase / Q000378:metabolism*; D010168:Palmitates / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D010168:Palmitates; D012997:Solvents; C032207:ethylhexyl palmitate; C093666:Lipozyme; D008049:Lipase