The conserved metalloprotease invadolysin localizes to the surface of lipid droplets.
Invadolysin is a metalloprotease conserved in many different organisms, previously shown to be essential in Drosophila with roles in cell division and cell migration. The gene seems to be ubiquitously expressed and four distinct splice variants have been identified in human cells but not in most other species examined. Immunofluorescent detection of human invadolysin in cultured cells reveals the protein to be associated with the surface of lipid droplets. By means of subcellular fractionation, we have independently confirmed the association of invadolysin with lipid droplets. We thus identify invadolysin as the first metalloprotease located on these dynamic organelles. In addition, analysis of larval fat-body morphological appearance and triglyceride levels in the Drosophila invadolysin mutant suggests that invadolysin plays a role in lipid storage or metabolism.
Cobbe
Neville
N
University of Edinburgh, Queen's Medical Research Institute, Centre for Cardiovascular Science, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.
Marshall
Kathryn M
KM
Gururaja Rao
Shubha
S
Chang
Ching-Wen
CW
Di Cara
Francesca
F
Duca
Edward
E
Vass
Sharron
S
Kassan
Adam
A
Heck
Margarete M S
MM
D017398:Alternative Splicing / Q000187:drug effects / Q000235:genetics; D000818:Animals; D002460:Cell Line; D017124:Conserved Sequence; D004330:Drosophila / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology; D010802:Phylogeny; D021381:Protein Transport / Q000187:drug effects; D011554:Pseudopodia / Q000187:drug effects / Q000201:enzymology
D029721:Drosophila Proteins; D008055:Lipids; D019301:Oleic Acid; D008666:Metalloendopeptidases; C495212:invadolysin, Drosophila; C495213:invadolysin, human
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