Cytological techniques to study human female meiotic prophase.
Most of the human aneuploidies have a maternal origin. This feature makes the study of human female meiosis a fundamental topic to understand the reasons leading to this important social problem. Unfortunately, due to sample collection difficulties, not many studies have been performed on human female meiotic prophase. In this chapter we present a comprehensive collection of protocols that allows the study of human female meiotic prophase through different technical approaches using both spread and structurally preserved oocytes.
Roig
Ignasi
I
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Garcia-Caldés
Montserrat
M
D020732:Cytogenetic Analysis / Q000379:methods; D003584:Cytological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D010053:Ovary / Q000166:cytology* / Q000378:metabolism; D011418:Prophase / Q000235:genetics*