Enteric mucosal immune response might trigger the immunomodulation activity of Ganoderma lucidum polysaccharide in mice.
Ganoderma lucidum is a well-known traditional Chinese medicine and often used for improving quality of life. Ganoderma lucidum polysaccharide (GLP) is the main active component in the fungus. Recent researches have shown that oral administration of GLP could produce immunomodulation and antitumor activity. With a high molecular weight, GLP is not easy to be absorbed, and the pathway via enteric mucosal immune response might be important for the immunomodulation of GLP. To investigate the potential mechanism, Kunming mice, weighing 20 +/- 2 g, were used and their peripheral blood mononuclear cells (PBMC), intraepithelial lymphocytes (IEL) and Peyer's patches lymphocytes (PPL) were separated, and incubated with GLP at different dosages (250 microg/mL, 125 microg/mL, 62.5 microg/mL, 31.25 microg/mL). MTT, ELISA and RT-PCR were used to detect the effects of GLP on the proliferation of the lymphocytes, and expression of IL-10, IL-2 and TNF-alpha. The results showed that GLP could stimulate the proliferation of PBMC and enteric mucosal lymphocytes, and promote the production of IL-2 and IL-10. The RT-PCR results also showed a higher expression of TNF-alpha and IL-10 mRNA in the lymphocytes. The higher expression of IL-2, IL-10 and TNF-alpha from the lymphocytes was observed at GLP concentrations of 62.5 microg/mL and 125 microg/mL. In conclusion, enteric mucosal immune responses could be one of the important pathways for the immunomodulation activity of GLP.
Zhao
Hongyan
H
Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, PR China.
Luo
Yingying
Y
Lu
Cheng
C
Lin
Na
N
Xiao
Cheng
C
Guan
Shuhong
S
Guo
De-an
DA
Liu
Zhenli
Z
Ju
Dahong
D
He
Xiaojuan
X
Lu
Aiping
A
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010581:Peyer's Patches / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020940:Reishi / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D007376:Interleukin-2; D011134:Polysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10