title: [Construction of adeno-associated virus vector containing ANG-1 gene and its expression in pig mesenchymal stem cells].
abstract: OBJECTIVE
  To construct recombinant adeno-associated virus (rAAV) vector containing angiopoietin-1 (ANG-1) gene and to express the ANG-1 in targeting cells.
  
  
  METHODS
  ANG-1 cDNA was obtained from human spleen by RT-PCR and was inserted into AAV vectors to form rAAV ANG-1, the virus stocks in high titer were harvested. The rAAVANG-1 and rAAV GFP were transferred into pig mesenchymal stem cells and the expression of ANG-1 was detected by Western blot.
  
  
  RESULTS
  The cloned ANG-1 cDNA was 1515bp in length which was in accordance with that reported previously. Titration of rAAVANG-1 stock was 9 X 10(11)v.g/ml. The expression of ANG-1 gene was detected in transfected cells. Forty-eight hours after rAAV GFP was transfected into mesenchymal stem cells, 55% cells expressed GFP.
  
  
  CONCLUSIONS
  The constructed rAAV ANG-1 vector has successfully transfered and expressed in pig mesenchymal stem cells.
authors.affiliation: Department of Cardiothoracic Surgery, Taizhou Hospital, Linhai 317000, China.
authors.forename: Cheng-chu
authors.initials: CC
authors.lastname: ZHU
authors.forename: Shi-lin
authors.initials: SL
authors.lastname: CHEN
authors.forename: Yu-qing
authors.initials: YQ
authors.lastname: LIU
authors.forename: Li-jiang
authors.initials: LJ
authors.lastname: TANG
authors.forename: Wei-guang
authors.initials: WG
authors.lastname: BAO
mesh_terms: D042683:Angiopoietin-1 / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine; D014162:Transfection
chemical_list: D042683:Angiopoietin-1; D018076:DNA, Complementary; D011994:Recombinant Proteins