Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force.
We present a method to discriminate normal oocytes in an optoelectrofluidic platform based on the optically induced positive dielectrophoresis (DEP) for in vitro fertilization. By combining the gravity with a pulling-up DEP force that is induced by dynamic image projected from a liquid crystal display, the discrimination performance could be enhanced due to the reduction in friction force acting on the oocytes that are relatively large and heavy cells being affected by the gravity field. The voltage condition of 10 V bias at 1 MHz was applied for moving normal oocytes. The increased difference of moving velocity between normal and starved abnormal oocytes allows us to discriminate the normal ones spontaneously under the moving image pattern. This approach can be useful to develop an automatic and interactive selection tool of fertilizable oocytes.
Hwang
Hyundoo
H
Department of Bio and Brain Engineering, College of Life Science and Bioengineering, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Lee
Do-Hyun
DH
Choi
Wonjae
W
Park
Je-Kyun
JK
J Phys Chem B. 2008 Aug 14;112(32):9903-8
18646802
Hum Reprod Update. 2003 Sep-Oct;9(5):451-61
14640377
Biophys J. 1999 May;76(5):2833-42
10233099
Med Biol Eng Comput. 2005 Jan;43(1):150-4
15742734
Biomed Microdevices. 2008 Jun;10(3):337-45
18071907
Lab Chip. 2005 Jan;5(1):86-90
15616744
J Microelectromech Syst. 2008 Apr;17(2):342-350
19079767
Lab Chip. 2005 Nov;5(11):1229-32
16234945
Biochim Biophys Acta. 1987 Dec 11;905(2):454-64
3689789
Science. 2002 Jun 21;296(5576):2188-90
12077406
Lab Chip. 2009 Jan 21;9(2):199-206
19107274
Electrophoresis. 2008 Mar;29(6):1203-12
18297658
Lab Chip. 2004 Jun;4(3):186-90
15159776
Electrophoresis. 2002 Jul;23(14):2174-83
12210221
Lab Chip. 2005 Jan;5(1):91-6
15616745
J Pharm Biomed Anal. 1997 Jun;15(9-10):1443-7
9226574
IEEE Trans Biomed Eng. 2001 May;48(5):570-8
11341531
Nature. 2005 Jul 21;436(7049):370-2
16034413