Sequences of the cytochrome C oxidase subunit I (COI) gene are suitable for species identification of Korean Calliphorinae flies of forensic importance (Diptera: Calliphoridae).
Calliphorinae fly species are important indicators of the postmortem interval especially during early spring and late fall in Korea. Although nucleotide sequences of various Calliphorinae fly species are available, there has been no research on the cytochrome c oxidase subunit I (COI) nucleotide sequences of Korean Calliphorinae flies. Here, we report the full-length sequences of the COI gene of four Calliphorinae fly species collected in Korea (five individuals of Calliphora vicina, five Calliphora lata, four Triceratopyga calliphoroides and three Aldrichina grahami). Each COI gene was amplified by polymerase chain reaction and directly sequenced and the resulting nucleotide sequences were aligned and analyzed by MEGA4 software. The results indicate that COI nucleotide sequences can be used to distinguish between these four species. Our phylogenetic result coincides with recent taxonomic views on the subfamily Calliphorinae in that the genera Aldrichina and Triceratopyga are nested within the genus Calliphora.
Park
Seong Hwan
SH
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA.
Zhang
Yong
Y
Piao
Huguo
H
Yu
Dong Ha
DH
Jeong
Hyun Ju
HJ
Yoo
Ga Young
GY
Jo
Tae-Ho
TH
Hwang
Juck-Joon
JJ
D000818:Animals; D001483:Base Sequence; D004175:Diptera / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004772:Entomology; D018732:Forensic Anthropology; D049429:Forensic Pathology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D013045:Species Specificity
D003576:Electron Transport Complex IV