title: Some Haliotrema (Monogenea: Dactylogyridae) from cultured grouper (Epinephelus Spp.) with emphasis on the phylogenetic position of Haliotrema cromileptis.
abstract: Three Haliotrema spp. are reported from the Vietnamese grouper. Morphological and morphometric characters show minor deviations from original descriptions of H. cromileptis Young, 1968 and H. epinepheli Young, 1968 . The third encountered species ( Haliotrema sp.) appears to be new to science. Genetically, H. cromileptis clusters with Bravohollisia , Pseudohaliotrema , and Haliotrema . The group is well supported by partial large subunit rDNA (LSU), complete small subunit rDNA (SSU), and partial SSU + ITS1 rDNA analyses. Ingroup phylogenetic relationships are not well resolved. Haliotrema cromileptis , H. fleti Young, 1968 , and Pseudohaliotrema sphincteroporus Yamaguti, 1953 are closely related to a monophyletic group of 5 Haliotrema spp. characterized by bell- or horn-shaped bases of the male copulatory organ (MCO), which contains an accessory piece. Based on SSU rDNA, H. cromileptis is a sister species to P. sphincteroporus and, together, they form a clade to 3 other Haliotrema spp. characterized by a bell-shape based MCO with an accessory piece. Data analysis conducted on partial SSU + ITS1 rDNA confirms the close phylogenetic relationship of H. cromileptis , H. fleti , H. chenhsintaoi Zhang, 2001 (possessing a horn-shaped base of the MCO), and Bravohollisia rosetta Lin, 1995. However, because major differences in diagnostic characters exist, this genetic relationship needs further elucidation.
authors.affiliation: Institute for Biotechnology and Environment, University of Nha Trang, Nha Trang, Viet Nam.
authors.forename: Binh T
authors.initials: BT
authors.lastname: Dang
authors.forename: Arne
authors.initials: A
authors.lastname: Levsen
authors.forename: Christoffer
authors.initials: C
authors.lastname: Schander
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Bristow
mesh_terms: D000818:Animals; D001492:Bass / Q000469:parasitology*; D001499:Bayes Theorem; D016054:DNA, Protozoan / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005393:Fish Diseases / Q000469:parasitology*; D005398:Fisheries; D010802:Phylogeny; D010986:Platyhelminths / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D017421:Sequence Analysis / Q000662:veterinary; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*; D014744:Vietnam
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal