Molecular identification of oriental medicinal plant Anemarrhena asphodeloides Bunge ('Jimo') by multiplex PCR.
Anemarrhena asphodeloides Bunge (Jimo) is one of the most popular and valuable plant species in many countries, including China, Korea, and Japan. The current commercial products such as Sagan, Chog-chag-yag, and Shig-ban-pung which are most similar to Jimo roots, were used for more reliable authentication method. Polymerase chain reaction (PCR) analysis of the trnL-F region has been proved to be an appropriate method for the identification of species in the A. asphodeloides genus. A single nucleotide polymorphism (SNP) has been identified in Jimo, Sagan, Chog-chag-yag, and Shig-ban-pung. The specific PCR primers were designed from the SNP to differentiate the A. asphodeloides (Jimo) from Sagan, Chog-chag-yag, and Shig-ban-pung via multiplex PCR. The established multiplex-PCR method for the rapid detection of the Jimo in a single reaction was determined to be effective for the differentiation of Jimo (A. asphodeloides). We therefore present an effective method for the genetic identification of the A. asphodeloides.
Jigden
Baigalmaa
B
Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 449-701, South Korea.
Wang
Hongtao
H
Samdan
Narantuya
N
Yang
Deok-Chun
DC
D031403:Anemarrhena / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D056426:Genetic Loci; D008518:Medicine, East Asian Traditional; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
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