Expression pattern of stemness-related genes in human endometrial and endometriotic tissues.
Endometriosis is a chronic disease characterized by the presence of ectopic endometrial tissue outside of the uterus with mixed traits of benign and malignant pathology. In this study we analyzed in endometrial and endometriotic tissues the differential expression of a panel of genes that are involved in preservation of stemness status and consequently considered as markers of stem cell presence. The expression profiles of a panel of 13 genes (SOX2, SOX15, ERAS, SALL4, OCT4, NANOG, UTF1, DPPA2, BMI1, GDF3, ZFP42, KLF4, TCL1) were analyzed by reverse transcription-polymerase chain reaction in human endometriotic (n = 12) and endometrial samples (n = 14). The expression of SALL4 and OCT4 was further analyzed by immunohistochemical methods. Genes UTF1, TCL1, and ZFP42 showed a trend for higher frequency of expression in endometriosis than in endometrium (P < 0.05 for UTF1), whereas GDF3 showed a higher frequency of expression in endometrial samples. Immunohistochemical analysis revealed that SALL4 was expressed in endometriotic samples but not in endometrium samples, despite the expression of the corresponding mRNA in both the sample groups. This study highlights a differential expression of stemness-related genes in ectopic and eutopic endometrium and suggests a possible role of SALL4-positive cells in the pathogenesis of endometriosis.
Forte
Amalia
A
Department of Experimental Medicine, Second University of Naples, Italy.
Schettino
Maria Teresa
MT
Finicelli
Mauro
M
Cipollaro
Marilena
M
Colacurci
Nicola
N
Cobellis
Luigi
L
Galderisi
Umberto
U
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016000:Cluster Analysis; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D000090062:Kruppel-Like Factor 4; D008875:Middle Aged; D015999:Multivariate Analysis; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C472575:SALL4 protein, human; D014157:Transcription Factors
FASEB J. 2007 Aug;21(10):2273-84
17360849
Fertil Steril. 2008 Nov;90(5):1559-70
18993168
Crit Rev Eukaryot Gene Expr. 2006;16(3):211-31
17073552
Bull Exp Biol Med. 2008 Apr;145(4):539-43
19110612
Stem Cells. 2007 Aug;25(8):2082-6
17464086
Exp Hematol. 2004 Jul;32(7):585-98
15246154
Nat Cell Biol. 2007 Jun;9(6):625-35
17515932
Front Biosci. 2005 Jan 01;10:569-75
15574393
Endocr J. 2008 Oct;55(5):795-810
18580040
Fertil Steril. 2019 Oct;112(4 Suppl1):e93-e115
31623748
Nature. 2007 Jul 19;448(7151):313-7
17554338
PLoS Comput Biol. 2006 Nov 24;2(11):e158
17121458
Stem Cells. 2008 Dec;26(12):3068-74
18787205
Hum Reprod. 2007 Jan;22(1):45-51
16923745
J Reprod Immunol. 2002 May-Jun;55(1-2):49-64
12062821
Mol Endocrinol. 2009 Feb;23(2):265-75
19074548
Biol Reprod. 2004 Jun;70(6):1738-50
14766732
Mol Hum Reprod. 2006 Jan;12(1):7-10
16421218
Cell. 2006 Aug 25;126(4):663-76
16904174
Exp Cell Res. 2009 Feb 1;315(3):498-507
19084517
Biol Reprod. 2009 Jun;80(6):1136-45
19228591
Am J Obstet Gynecol. 1980 Jun 1;137(3):327-31
7377252
Cell Cycle. 2008 Mar 15;7(6):725-8
18239456
JAMA. 2004 Jul 7;292(1):81-5
15238594
Stem Cells. 2006 Dec;24(12):2685-91
16916925
Int J Hematol. 2005 May;81(4):294-300
15914357
Mol Cell Biol. 1999 Aug;19(8):5453-65
10409735
Stem Cells. 2006 Jun;24(6):1529-38
16456137
J Clin Endocrinol Metab. 2007 Aug;92(8):3261-7
17519303
Hum Reprod. 2007 Apr;22(4):919-26
17208945
Int J Cancer. 2006 Aug 1;119(3):556-62
16506222
Dev Biol. 2005 Aug 1;284(1):194-203
15993878
Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19756-61
19060217
Stem Cells. 2007 Jul;25(7):1675-80
17379765
Zentralbl Gynakol. 2003 Jul-Aug;125(7-8):235-8
14505255
Cancer. 2005 Nov 15;104(10):2255-65
16228988
Nat Cell Biol. 2006 Oct;8(10):1114-23
16980957
Mol Cell Biol. 2006 Oct;26(20):7772-82
16954384
J Transl Med. 2007 Nov 15;5:57
18005405
J Biol Chem. 2005 Jul 1;280(26):24371-9
15863505
Stem Cell Res. 2009 May;2(3):211-8
19393592
J Pathol. 2008 Jul;215(3):317-29
18473332
EMBO J. 1998 Apr 1;17(7):2019-32
9524124
Cell Stem Cell. 2008 Nov 6;3(5):543-54
18804426
Exp Cell Res. 2008 Jun 10;314(9):1937-44
18439579
J Biol Chem. 2006 Nov 3;281(44):33554-65
16951404
J Cell Biol. 2007 Sep 10;178(6):913-24
17785516
N Engl J Med. 2009 Jan 15;360(3):268-79
19144942
Cancer Res. 2006 Jan 15;66(2):820-7
16424014
PLoS One. 2008;3(10):e3406
18923680
Cell Res. 2007 Jan;17(1):42-9
17211451
Stem Cells. 2005 Nov-Dec;23(10):1535-40
16081664
J Biol Chem. 2006 Aug 25;281(34):24090-4
16840789
Biochem Biophys Res Commun. 2001 Jul 27;285(4):945-53
11467843
PLoS One. 2006 Dec 20;1:e26
17183653
Hum Reprod. 2008 Apr;23(4):775-82
18281244
Biol Reprod. 2007 Oct;77(4):681-7
17625110