title: Aqueous extract of brazilian green propolis: primary components, evaluation of inflammation and wound healing by using subcutaneous implanted sponges.
abstract: Propolis is a chemically complex resinous bee product which has gained worldwide popularity as a means to improve health condition and prevent diseases. The main constituents of an aqueous extract of a sample of green propolis from Southeast Brazil were shown by high performance liquid chromatography/mass spectroscopy/mass spectroscopy to be mono- and di-O-caffeoylquinic acids; phenylpropanoids known as important constituents of alcohol extracts of green propolis, such as artepillin C and drupanin were also detected in low amounts in the aqueous extract. The anti-inflammatory activity of this extract was evaluated by determination of wound healing parameters. Female Swiss mice were implanted subcutaneously with polyesther-polyurethane sponge discs to induce wound healing responses, and administered orally with green propolis (500 mg kg(-1)). At 4, 7 and 14 days post-implantation, the fibrovascular stroma and deposition of extracellular matrix were evaluated by histopathologic and morphometric analyses. In the propolis-treated group at Days 4 and 7 the inflammatory process in the sponge was reduced in comparison with control. A progressive increase in cell influx and collagen deposition was observed in control and propolis-treated groups during the whole period. However, these effects were attenuated in the propolis-treated group at Days 4 and 7, indicating that key factors of the wound healing process are modulated by propolis constituents.
authors.affiliation: Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais-UFMG, Belo Horizonte, Brazil.
authors.forename: Sandra Aparecida Lima
authors.initials: SA
authors.lastname: de Moura
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Negri
authors.forename: Antônio
authors.initials: A
authors.lastname: Salatino
authors.forename: Luiza Dias da Cunha
authors.initials: LD
authors.lastname: Lima
authors.forename: Luana Pereira Antunes
authors.initials: LP
authors.lastname: Dourado
authors.forename: Juliana Barros
authors.initials: JB
authors.lastname: Mendes
authors.forename: Silvia Passos
authors.initials: SP
authors.lastname: Andrade
authors.forename: Mônica Alves Neves Diniz
authors.initials: MA
authors.lastname: Ferreira
authors.forename: Denise Carmona
authors.initials: DC
authors.lastname: Cara
references.citation: Clin Exp Dermatol. 2007 Nov;32(6):709-15
references.pmid: 17953639
references.citation: Eur J Pharmacol. 2008 Jun 10;587(1-3):296-301
references.pmid: 18474366
references.citation: Anticancer Res. 1997 Sep-Oct;17(5A):3565-8
references.pmid: 9413203
references.citation: Evid Based Complement Alternat Med. 2005 Jun;2(2):201-207
references.pmid: 15937561
references.citation: Microvasc Res. 1997 Nov;54(3):253-61
references.pmid: 9441896
references.citation: Wound Repair Regen. 2003 Jan-Feb;11(1):25-34
references.pmid: 12581424
references.citation: Evid Based Complement Alternat Med. 2005 Mar;2(1):33-38
references.pmid: 15841276
references.citation: Evid Based Complement Alternat Med. 2005 Mar;2(1):85-92
references.pmid: 15841282
references.citation: Planta Med. 2007 Nov;73(14):1469-74
references.pmid: 17948188
references.citation: J Agric Food Chem. 2006 Jun 14;54(12):4095-101
references.pmid: 16756331
references.citation: World J Gastroenterol. 2008 Aug 14;14(30):4763-70
references.pmid: 18720536
references.citation: Wound Repair Regen. 2001 Nov-Dec;9(6):522-30
references.pmid: 11896995
references.citation: Histochem J. 1979 Jul;11(4):447-55
references.pmid: 91593
references.citation: Histol Histopathol. 2006 Dec;21(12):1263-70
references.pmid: 16977576
references.citation: Phytochem Anal. 2008 Jan-Feb;19(1):32-9
references.pmid: 17654520
references.citation: Phytomedicine. 2001 Jan;8(1):16-23
references.pmid: 11292234
references.citation: J Invertebr Pathol. 2008 Mar;97(3):273-81
references.pmid: 18054037
references.citation: Anal Chim Acta. 2009 Mar 2;635(1):115-20
references.pmid: 19200487
references.citation: J Ethnopharmacol. 2005 Sep 14;100(3):276-83
references.pmid: 15899563
references.citation: Drugs Exp Clin Res. 1993;19(5):197-203
references.pmid: 7513636
references.citation: J Agric Food Chem. 2008 Nov 12;56(21):10105-14
references.pmid: 18837557
references.citation: Am J Pathol. 2001 Oct;159(4):1255-62
references.pmid: 11583953
references.citation: J Ethnopharmacol. 2005 Oct 31;102(1):37-45
references.pmid: 16054317
references.citation: Food Chem. 2008 May 15;108(2):749-59
references.pmid: 26059157
references.citation: Phytother Res. 2001 Nov;15(7):561-71
references.pmid: 11746834
references.citation: J Agric Food Chem. 2002 Jun 5;50(12):3579-85
references.pmid: 12033832
references.citation: FASEB J. 2005 Sep;19(11):1561-3
references.pmid: 16014399
references.citation: Life Sci. 2007 Jan 2;80(4):370-7
references.pmid: 17046025
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 15;858(1-2):96-105
references.pmid: 17766198
references.citation: Biol Pharm Bull. 2004 Nov;27(11):1797-803
references.pmid: 15516726
references.citation: J Ethnopharmacol. 2007 Apr 4;110(3):567-71
references.pmid: 17126509
references.citation: Int J Biochem Cell Biol. 1998 Sep;30(9):1019-30
references.pmid: 9785465