title: In vitro and in vivo anti-photoaging effects of an isoflavone extract from soybean cake.
abstract: BACKGROUND
  Soy has been used in traditional Chinese medicine for thousands of years for its health and nutritional benefits, as well as to treat and care for the skin. Advanced skin care research has shown that soy isoflavone and genistein are effective in reducing damage to the skin from the sun.
  
  
  OBJECTIVE
  To study the protective effects of isoflavone extract from soybean cake against the UVB-induced skin damage.
  
  
  METHODS
  The in vitro effects and possible mechanisms of soybean extract on UVB protection were determined in HaCaT cells. In the in vivo study, ICR-Foxn/(nu) mice were irradiated with UVB. The epidermal thickness, catalase and the expressions of cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) were detected to evaluate the antioxidant and anti-inflammatory activities of the isoflavone extract.
  
  
  RESULTS
  Our in vitro studies showed that UVB-induced HaCaT cell death and the phosphorylation of p38, JNK, and ERK1/2 decreased in the presence of isoflavone extract. In the in vivo studies, we found that the topical application of isoflavone extract before UVB irritation decreased the epidermal thickness and the expressions of COX-2 and PCNA and increased catalase concentration. These results showed anti-photoaging effect of isoflavone extract from soybean cake involved the inhibition of UVB-induced apoptosis and inflammation.
  
  
  CONCLUSIONS
  It is shown that isoflavone extract from soybean cake may be functional cosmeceutical candidate for skin photoaging.
authors.affiliation: Department of Dermatology, Changhua Christian Hospital, Changhua Hsien, Taiwan, ROC.
authors.forename: Tsu-Man
authors.initials: TM
authors.lastname: Chiu
authors.forename: Chieh-Chen
authors.initials: CC
authors.lastname: Huang
authors.forename: Tzu-Ju
authors.initials: TJ
authors.lastname: Lin
authors.forename: Jia-You
authors.initials: JY
authors.lastname: Fang
authors.forename: Nan-Lin
authors.initials: NL
authors.lastname: Wu
authors.forename: Chi-Feng
authors.initials: CF
authors.lastname: Hung
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004890:Erythema; D019833:Genistein / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012867:Skin / Q000033:anatomy & histology / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D013025:Glycine max / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D018809:Proliferating Cell Nuclear Antigen; D019833:Genistein; D002374:Catalase; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2