title: Polymorphisms of NKG2D ligands: diverse RAET1/ULBP genes in northeastern Thais.
abstract: Unique long 16 (UL-16)-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor, NKG2D. The human RAET1/ULBP gene family is identified as ten members (RAET1E to N) with six loci encoding for potentially functional proteins. These are ULBP1 or RAET1I, ULBP2 or RAET1H, ULBP3 or RAET1N, and RAET1L, which are glycosylinositol phospholipid (GPI)-linked glycoproteins and ULBP4 or RAET1E and ULBP5 or RAET1G, which are transmembrane glycoproteins. The RAET1 products contain the alpha1 and alpha2 domains but lack the alpha3 domain and do not associate with beta2-microglobulin. RAET1/ULBPs have tissue-specific expressions, and some of them are also polymorphic. In the present study, polymorphic exons 2 and 3 of the RAET1E, G, H, I, L, and N were analyzed using sequence-based typing. One hundred and seventy-six unrelated healthy Northeastern Thais were included in this study. For RAET1E, RAET1G, RAET1H, and RAET1L, there were seven, two, five, and four single nucleotide polymorphisms (SNPs), respectively. Six of these are new SNPs, which are rare in this population. Of these, six new SNPs, two of two in RAET1E, two of three in RAET1H, and none of one in RAET1L are nonsynonymous substitutions. Interestingly, although the RAET1N is polymorphic in Caucasians, RAET1N and RAET1I had no variation in Thais indicating diverse RAET1 genes in different ethnic groups. These data provide the important basis for future analysis on the role of RAET1 genes in immune responses especially in cancer and infectious diseases.
authors.affiliation: Blood Transfusion Center, Faculty of Medicine, Khon Kaen University (KKU), Khon Kaen 40002, Thailand.
authors.forename: Amornrat V
authors.initials: AV
authors.lastname: Romphruk
authors.forename: Arunrat
authors.initials: A
authors.lastname: Romphruk
authors.forename: Taeko K
authors.initials: TK
authors.lastname: Naruse
authors.forename: Sarayot
authors.initials: S
authors.lastname: Raroengjai
authors.forename: Chintana
authors.initials: C
authors.lastname: Puapairoj
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inoko
authors.forename: Chanvit
authors.initials: C
authors.lastname: Leelayuwat
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D058851:GPI-Linked Proteins; D014644:Genetic Variation; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013785:Thailand
chemical_list: D002352:Carrier Proteins; D058851:GPI-Linked Proteins; D015395:Histocompatibility Antigens Class I; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C488760:RAET1E protein, human; C488761:RAET1G protein, human; C423231:ULBP1 protein, human; C423230:ULBP2 protein, human; C423229:ULBP3 protein, human
references.citation: J Biol Chem. 2007 Jun 29;282(26):18922-8
references.pmid: 17470428
references.citation: Immunogenetics. 1994;40(5):339-51
references.pmid: 7927538
references.citation: Science. 1998 Mar 13;279(5357):1737-40
references.pmid: 9497295
references.citation: Immunity. 2001 Aug;15(2):201-11
references.pmid: 11520456
references.citation: Immunity. 2002 Jul;17(1):19-29
references.pmid: 12150888
references.citation: PLoS One. 2007 Jun 13;2(6):e518
references.pmid: 17565371
references.citation: Immunol Rev. 2001 Jun;181:185-92
references.pmid: 11513139
references.citation: Eur J Immunol. 2003 Jan;33(1):194-203
references.pmid: 12594848
references.citation: Genomics. 2002 Jan;79(1):114-23
references.pmid: 11827464
references.citation: Immunity. 2001 Jul;15(1):83-93
references.pmid: 11485740
references.citation: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9452-7
references.pmid: 12878725
references.citation: Immunogenetics. 2001 May-Jun;53(4):279-87
references.pmid: 11491531
references.citation: Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12445-50
references.pmid: 8901601
references.citation: Int Immunol. 2008 Aug;20(8):981-91
references.pmid: 18544572
references.citation: Immunity. 2002 Jan;16(1):77-86
references.pmid: 11825567
references.citation: Clin Exp Immunol. 2008 Aug;153(2):205-13
references.pmid: 18505429
references.citation: J Immunol. 2002 Apr 1;168(7):3131-4
references.pmid: 11907062
references.citation: Science. 1992 Aug 14;257(5072):927-34
references.pmid: 1323878
references.citation: Immunity. 2001 Feb;14(2):123-33
references.pmid: 11239445
references.citation: Tissue Antigens. 2005 Apr;65(4):301-69
references.pmid: 15787720
references.citation: Nat Immunol. 2000 Aug;1(2):119-26
references.pmid: 11248803
references.citation: Biochem Biophys Res Commun. 2001 Jul 13;285(2):235-43
references.pmid: 11444831
references.citation: Biochem Biophys Res Commun. 2003 May 23;305(1):129-35
references.pmid: 12732206
references.citation: Eur J Immunol. 2001 May;31(5):1428-37
references.pmid: 11465099
references.citation: Science. 1999 Jul 30;285(5428):727-9
references.pmid: 10426993
references.citation: Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6259-63
references.pmid: 8022771
references.citation: J Immunol. 2002 Oct 15;169(8):4079-83
references.pmid: 12370332
references.citation: Science. 2001 Oct 19;294(5542):605-9
references.pmid: 11567106
references.citation: Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6879-84
references.pmid: 10359807
references.citation: Nat Immunol. 2001 Mar;2(3):255-60
references.pmid: 11224526
references.citation: J Immunol. 2004 Jul 15;173(2):1078-84
references.pmid: 15240696