Metabolic changes of salicylic acid-elicited Catharanthus roseus cell suspension cultures monitored by NMR-based metabolomics.
The effect of salicylic acid (SA) on the metabolic profile of Catharanthus roseus suspension cells throughout a time course (0, 6, 12, 24, 48 and 72 h after treatment) was investigated using NMR spectroscopy and multivariate data analysis. When compared to control cell lines, SA-treated cells showed a high level of sugars (glucose and sucrose) up to 48 h after treatment, followed by a dynamic change in amino acids, phenylpropanoids, and tryptamine. Additionally, one compound--2,5-dihydroxybenzoic-5-O-glucoside--was detected solely in SA-treated cells.
Mustafa
Natali Rianika
NR
Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.
Kim
Hye Kyong
HK
Choi
Young Hae
YH
Verpoorte
Robert
R
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D030008:Catharanthus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D005841:Gentisates / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055432:Metabolomics; D020005:Propanols / Q000032:analysis; D020156:Salicylic Acid / Q000378:metabolism*; D013997:Time Factors; D014363:Tryptamines / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D005841:Gentisates; D020005:Propanols; D014363:Tryptamines; C439395:1-phenylpropanol; C030820:tryptamine; D020156:Salicylic Acid
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