title: Monitoring by HPLC of chamomile flavonoids exposed to rat liver microsomal metabolism.
abstract: Three major flavonoid chamomile components (quercetin, apigenin-7-O-glucoside and rutin) were subjected to oxidative metabolism by cytochrome P-450 of rat liver microsomal preparations. Changes over time in their respective concentrations were followed using reversed-phase HPLC with UV detection. No clean-up had to be applied as only the specific flavonoid had to be separated from the background components originating from the rat liver microsome.Neither the concentration of apigenin-7-O-glucoside nor that of the diglycoside rutin decreased during one hour of exposure to rat microsomal treatment. In contrast, the concentration of quercetin, a lipophilic aglycon, decreased.Our analytical HPLC results complement the in silico calculated lipophilicity (logP) of these compounds; the relatively high lipophilicity of quercetin appears to predispose it to oxidative metabolism in order to decrease its fat solubility. In contrast the much less lipophilic compounds apigenin-7-O-glucoside and rutin were resistant in vitro to microsomal treatment.
authors.affiliation: Department of Cell Biology, Florida International University, Miami, FL, USA.
authors.forename: Georg
authors.initials: G
authors.lastname: Petroianu
authors.forename: Eva
authors.initials: E
authors.lastname: Szoke
authors.forename: Huba
authors.initials: H
authors.lastname: Kalász
authors.forename: Péter
authors.initials: P
authors.lastname: Szegi
authors.forename: Rudolf
authors.initials: R
authors.lastname: Laufer
authors.forename: Bernadett
authors.initials: B
authors.lastname: Benko
authors.forename: Ferenc
authors.initials: F
authors.lastname: Darvas
authors.forename: Kornélia
authors.initials: K
authors.lastname: Tekes
references.citation: J Agric Food Chem. 2008 Sep 10;56(17):8206-11
references.pmid: 18681440
references.citation: J Agric Food Chem. 2007 Nov 14;55(23):9470-8
references.pmid: 17939735
references.citation: Bioorg Med Chem Lett. 2006 Feb 15;16(4):1037-9
references.pmid: 16288868
references.citation: Praxis (Bern 1994). 1996 Nov 12;85(46):1476-81
references.pmid: 8984570
references.citation: Planta Med. 1990 Apr;56(2):179-81
references.pmid: 17221394
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):231-8
references.pmid: 14698259
references.citation: Phytochemistry. 2004 Aug;65(16):2323-32
references.pmid: 15381003