Cell wall remodeling in Arabidopsis stamen abscission zones: Temporal aspects of control inferred from transcriptional profiling.
Organ shedding requires cell separation within abscission zones (AZs). Functional genomic AZ studies have been limited by their small size and low incidence. Optimization of laser capture microdissection (LCM) for AZs and other specialized cell types in Arabidopsis1 allowed recent characterization of the floral stamen AZ transcriptome responding to a developmental shedding cue.2 Analyses focused on 551 AZ transcripts (AZ(551)) that were regulated at the highest statistical significance (p </= 0.0001) over five stages of stamen development spanning pre-pollination to organ shed.2 Here, we seek a fuller understanding of AZ integrity control by relaxing P value restrictions on statistical significance ten-fold to generate an expanded population of 1461 stamen transcripts (AZ(1461)). Cell wall remodeling functions in AZ(1461) are significantly over-represented relative to all transcripts represented on the whole genome GeneChip. Hierarchical clustering of gene expression data corresponding to cell wall-related transcripts suggests a temporal model for AZ remodeling in Arabidopsis stamens destined to abscise.
Lashbrook
Coralie C
CC
Iowa State University; Department of Genetics, Development and Cell Biology; Ames, Iowa USA.
Cai
Suqin
S
Plant Physiol. 1992 Mar;98(3):955-61
16668770
Plant Physiol. 2001 Jan;125(1):131-4
11154315
Plant Mol Biol. 2006 Jun;61(3):451-67
16830179
Mol Biol Evol. 2007 Apr;24(4):1045-55
17272678
Plant Mol Biol. 2004 May;55(3):311-4
15604683
Genes Dev. 2000 Jan 1;14(1):108-17
10640280
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
9843981
Mol Plant Microbe Interact. 2002 Apr;15(4):404-7
12026180
Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6574-8
11607483
Curr Opin Plant Biol. 2000 Feb;3(1):73-8
10679451
Plant Physiol. 2002 Mar;128(3):854-64
11891242
Plant Physiol. 2007 Aug;144(4):1693-6
17687051
Plant Mol Biol. 2004 Sep;56(2):309-23
15604746
Plant Physiol. 2008 Mar;146(3):1305-21
18192438
Phytochemistry. 2007 Jun;68(11):1510-20
17466346
Plant Mol Biol. 2001 Sep;47(1-2):55-72
11554480
Plant J. 1993 Jan;3(1):1-30
8401598
Plant Signal Behav. 2006 Nov;1(6):281-3
19704626
Ann Bot. 2005 Jul;96(1):91-9
15837720
Plant Cell Physiol. 2002 Dec;43(12):1421-35
12514239
Plant Cell. 2003 Oct;15(10):2296-307
12972671
J Exp Bot. 2005 Mar;56(413):817-23
15689341
Plant Physiol. 1995 Jan;107(1):87-100
11536663
Plant J. 2006 Nov;48(4):628-37
17026538