Oxidative DNA damage in patients with cataract.
OBJECTIVE
This study examines the levels of oxidative damage in patients with cataract.


METHODS
Blood samples were collected from 60 patients with cataract and 60 age- and gender-matched healthy individuals to measure 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels.


RESULTS
A significant difference was observed in leukocyte 8-OHdG levels in patients with cataract in comparison with healthy persons (p < 0.001). Similarly, a significant difference was observed in plasma MDA levels in patients with cataract in comparison with healthy persons (p<0.001). In addition, a significant correlation was found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r = 0.859, p < 0.001).


CONCLUSIONS
This study measured the oxidative DNA damage by measuring the 8-OHdG in the leukocyte DNA in patients with cataract. In addition, the level of MDA - a marker for lipid peroxidation - was measured to determine lipid peroxidation.
Ates
Orhan
O
Department of Ophthalmology, Ataturk University, Erzurum, Turkey. orhanates69@hotmail.com
Alp
Hakan Hamit
HH
Kocer
Ibrahim
I
Baykal
Orhan
O
Salman
Ilknur A
IA
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000375:Aging / Q000502:physiology; D002386:Cataract / Q000097:blood / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine