[Establishment of RT- LAMP for rapid detection of foot-and-mouth disease virus].
A rapid detection of foot-and-mouth disease virus (FMDV) was established by using reverse transcription loop-mediated isothermal amplification (RT-LAMP) method, meanwhile its specificity and sensitivity were assessed. The results showed that the FMDV RNA could be amplified by incubation at 65degrees C for only 1h using six primers designed based on FMDV polyprotein gene and the amplification products could be detected easily by naked-eye. There is no cross reaction with other virus such as SVDV, SFV and PPV by detecting their RNA samples. The detection limit of this method was found to be 10(-5) dilution of RNA sample which was 100-fold higher than that of PCR and 10-fold higher than real-time PCR.
Li
Jian
J
Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai, China. lijiancq@yahoo.com.cn
Chen
Qin
Q
Xiong
Wei
W
Fang
Xue-En
XE
D000818:Animals; D017931:DNA Primers; D005536:Foot-and-Mouth Disease / Q000517:prevention & control / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013555:Swine Vesicular Disease / Q000821:virology
D017931:DNA Primers; D012367:RNA, Viral