title: The chromosomal localization, expression pattern and polymorphism analysis of porcine FSCN1 gene differently expressed from LongSAGE library.
abstract: Fascin homologue 1 (FSCN1) has established roles in cell adhesion, motility, and cell-cell interactions. Our LongSAGE analysis suggested that FSCN1 was potentially differentially expressed in prenatal pig skeletal muscle. We have cloned the genomic DNA and mRNA sequence of FSCN1 gene and mapped it to SSC3p16-p17. The FSCN1 gene was differently expressed during prenatal skeletal muscle development and exhibited different expression pattern between Tongcheng and Landrace pigs. In Tongcheng pigs, FSCN1 expression was similar at 33 and 65 days post conception (dpc), and then sharply increased to a peak at 90 dpc. In Landrace pigs, however, expression increased between 33 and 65 dpc, peaked at 65 dpc, and was down-regulated thereafter. Significantly different expression levels between Tongcheng and Landrace were observed at 65 and 90 dpc. In postnatal pigs, it was strongly expressed only in the brain, but weakly in skeletal muscle and other tissues. We initially identified 32 SNPs through genomic DNA of FSCN1 gene. Association analysis suggested that the 6840(C/T) mutation was significantly associated with the age at market weight (AGE) (p = 0.0004), average day gain from birth to market (ADG1) (p = 0.0002), and average day gain at testing period (ADG2) (p < 0.0001). Our study suggested that FSCN1 gene plays an in prenatal skeletal muscle development and was a candidate gene for meat production trait.
authors.affiliation: Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: Zh L
authors.initials: ZhL
authors.lastname: Tang
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Sh L
authors.initials: ShL
authors.lastname: Yang
authors.forename: Y L
authors.initials: YL
authors.lastname: Mu
authors.forename: W T
authors.initials: WT
authors.lastname: Cui
authors.forename: H
authors.initials: H
authors.lastname: Ao
authors.forename: K
authors.initials: K
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002352:Carrier Proteins / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005787:Gene Frequency / Q000235:genetics; D015723:Gene Library; D016678:Genome / Q000235:genetics; D007438:Introns / Q000235:genetics; D008460:Meat; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D008840:Microfilament Proteins; D012333:RNA, Messenger; C020324:fascin
references.citation: Gene Expr Patterns. 2004 Oct;4(6):637-43
references.pmid: 15465486
references.citation: J Cancer Res Clin Oncol. 2008 Sep;134(9):947-51
references.pmid: 18343949
references.citation: FASEB J. 2005 Mar;19(3):404-6
references.pmid: 15629888
references.citation: J Anat. 1983 Sep;137 (Pt 2):235-45
references.pmid: 6630038
references.citation: FEBS Lett. 1997 Sep 8;414(2):381-6
references.pmid: 9315724
references.citation: Mol Biol Cell. 2007 Oct;18(10):3928-40
references.pmid: 17671164
references.citation: Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12257-62
references.pmid: 12213963
references.citation: J Biol Chem. 1995 May 5;270(18):10764-70
references.pmid: 7738015
references.citation: Genome Biol. 2007;8(6):R115
references.pmid: 17573972
references.citation: Mol Genet Genomics. 2006 Sep;276(3):264-72
references.pmid: 16802157
references.citation: J Histochem Cytochem. 2008 Feb;56(2):193-9
references.pmid: 17998567
references.citation: Bioessays. 2002 Apr;24(4):350-61
references.pmid: 11948621
references.citation: J Anim Sci. 2005 Feb;83(2):308-15
references.pmid: 15644501
references.citation: Dis Markers. 2007;23(3):153-60
references.pmid: 17473384
references.citation: Exp Cell Res. 2002 Apr 15;275(1):92-109
references.pmid: 11925108
references.citation: Trends Cell Biol. 2004 Aug;14(8):452-60
references.pmid: 15308212
references.citation: Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9115-9
references.pmid: 8415664
references.citation: Bioinformatics. 2000 Jun;16(6):558-9
references.pmid: 10980153
references.citation: Neurosci Lett. 2005 Jun 10-17;381(1-2):169-74
references.pmid: 15882811
references.citation: J Histochem Cytochem. 2007 Sep;55(9):955-62
references.pmid: 17510372
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Cytogenet Cell Genet. 1998;82(3-4):182-8
references.pmid: 9858812