Isolation of deletion alleles by G4 DNA-induced mutagenesis.
Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.
Pontier
Daphne B
DB
Hubrecht Institute-Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Utrecht, The Netherlands.
Kruisselbrink
Evelien
E
Guryev
Victor
V
Tijsterman
Marcel
M
D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D054856:G-Quadruplexes; D017353:Gene Deletion; D016296:Mutagenesis
D004247:DNA
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