title: Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers.
abstract: Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated that BM-MSCs expressed myogenic (desmin, myogenin, myosin IIa, and alpha-SMA), neurogenic (gamma-enolase, MAP2a,b, c-fos, nestin, GFAP and beta III tubulin), and osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, BMP-2, BMP-4 and type I collagen) markers without stimulation towards differentiation. These expression patterns indicated why these cells can easily differentiate into multiple lineages both in vitro and in vivo. Ultrastructural characteristics of rBM-MSCs showed more developed and metabolically active cells.
authors.affiliation: Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, 41380, Kocaeli, Turkey. ekaraoz@hotmail.com
authors.forename: Erdal
authors.initials: E
authors.lastname: Karaoz
authors.forename: Ayça
authors.initials: A
authors.lastname: Aksoy
authors.forename: Selda
authors.initials: S
authors.lastname: Ayhan
authors.forename: Ayla Eker
authors.initials: AE
authors.lastname: Sariboyaci
authors.forename: Figen
authors.initials: F
authors.lastname: Kaymaz
authors.forename: Murat
authors.initials: M
authors.lastname: Kasap
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
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