The molecular mechanisms of cell death in the course of transient ischemia are differentiated in evolutionary distinguished brain structures.
There is large body of evidence suggesting distinct susceptibility to ischemia in various brain regions. However, the reason for this remains unexplained. Comparative studies of programmed cell death (PCD) pathways indicate their differentiated evolutional origin. The caspase-independent pathway is regarded as an older, whereas the caspase-dependent--as more advanced. In our study we address the question of whether there are any characteristic differences in the activation and course of PCD in phylogenetically and morphologically distinguished brain structures after transient focal ischemia. Using Western blot, we studied changes in expression of caspases: 3, 8, 9, and AIF in the frontoparietal neocortex, archicortex (CA1 and CA2 sectors of the hippocampus) and striatum, during reperfusion after 1 h occlusion of the middle cerebral artery. The caspase and AIF expression were differentiated between the studied structures. The activation of only the caspase-dependent pathway was observed in the neocortex. In the archicortex and striatum both caspase-dependent and caspase-independent pathways were activated, although in the latter the extrinsic apoptotic pathway was not activated. In summary, it is conceivable that structures of different evolutionary origin undergo cell-death processes with the participation of phylogenetically distinguished mechanisms. The previously reported unequal susceptibility to ischemia may co-exist with activation of different cell death pathways.
Lietzau
Grazyna
G
Department of Anatomy and Neurobiology, Medical University of Gdansk, 1 Debinki Street, 80-211, Gdansk, Poland.
Kowiański
Przemysław
P
Karwacki
Zbigniew
Z
Dziewiatkowski
Jerzy
J
Witkowska
Małgorzata
M
Sidor-Kaczmarek
Justyna
J
Moryś
Janusz
J
D000818:Animals; D005075:Biological Evolution; D015153:Blotting, Western; D001921:Brain / Q000473:pathology*; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000235:genetics*; D002560:Cerebrovascular Circulation / Q000502:physiology; D005260:Female; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000235:genetics* / Q000473:pathology*; D008297:Male; D009474:Neurons / Q000473:pathology; D010802:Phylogeny; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology; D015398:Signal Transduction / Q000235:genetics
D020169:Caspases
Brain Res Rev. 2007 Apr;54(1):34-66
17222914
J Comp Neurol. 2005 Jun 20;487(1):107-18
15861457
Cell Mol Neurobiol. 2006 Oct-Nov;26(7-8):1057-83
16710759
J Cereb Blood Flow Metab. 2004 Apr;24(4):458-66
15087715
Neuropathol Appl Neurobiol. 2003 Oct;29(5):472-81
14507339
Biochem Soc Trans. 2006 Dec;34(Pt 6):1283-6
17073802
Nature. 1999 Feb 4;397(6718):441-6
9989411
Eur J Neurosci. 2004 Nov;20(10):2541-54
15548198
J Neurosci. 1999 Jul 15;19(14):5932-41
10407032
Brain Res Mol Brain Res. 2004 Aug 23;127(1-2):105-14
15306126
Trends Neurosci. 1999 Sep;22(9):391-7
10441299
Cell Death Differ. 1999 Jun;6(6):516-24
10381654
Stroke. 2004 Sep;35(9):2189-94
15256676
J Comp Neurol. 2004 Feb 9;469(3):325-39
14730585
Brain Res Mol Brain Res. 2001 Apr 18;89(1-2):133-46
11311984
Brain. 2001 Jan;124(Pt 1):20-9
11133784
J Neurosci. 1998 Jul 1;18(13):4914-28
9634557
Biochimie. 2002 Feb-Mar;84(2-3):215-22
12022952
Stroke. 1986 May-Jun;17(3):472-6
3715945
J Neurosci. 2006 Sep 27;26(39):9956-66
17005859
J Mol Histol. 2007 Aug;38(4):295-302
17551674
Stroke. 2003 Jan;34(1):214-23
12511777
Prog Neuropsychopharmacol Biol Psychiatry. 2003 Apr;27(2):199-214
12657360
J Cell Biol. 2002 Aug 5;158(3):507-17
12147675
Exp Neurol. 2009 Jul;218(1):41-6
19348795
Brain Res Bull. 2005 Sep 15;66(4-6):297-302
16144605
Neurobiol Dis. 2005 Oct;20(1):27-37
16137564
J Neurosci. 2005 Nov 2;25(44):10262-72
16267234
Neurobiol Dis. 2007 Sep;27(3):354-61
17681771
Nat Neurosci. 2002 Dec;5(12):1279-87
12411960
J Neurosci. 1998 May 15;18(10):3659-68
9570797
BMC Genomics. 2007 Oct 15;8:370
17937787
NeuroRx. 2004 Jan;1(1):17-25
15717004
Cerebrovasc Dis. 2006;21 Suppl 2:9-20
16651810
J Neurosci. 2001 Sep 15;21(18):7127-34
11549723
FASEB J. 2000 Apr;14(5):729-39
10744629
Cell Death Differ. 1999 Nov;6(11):1028-42
10578171
Cerebrovasc Dis. 2006;21 Suppl 2:71-9
16651817
Brain Res Mol Brain Res. 2000 Jan 10;75(1):143-9
10648898
Brain Res. 2003 Aug 29;982(2):146-55
12915250
Drug Resist Updat. 2005 Jun;8(3):163-70
15946892
Life Sci. 1999;64(13):1099-108
10210272
Brain Res. 2003 Aug 29;982(2):228-40
12915258
Brain Res Bull. 2002 Feb-Mar 1;57(3-4):243-55
11922968
Brain Res Brain Res Protoc. 2004 Apr;13(1):11-7
15063836
Philos Trans R Soc Lond B Biol Sci. 2001 Oct 29;356(1414):1583-98
11604125
FEBS Lett. 2004 Jan 16;557(1-3):14-20
14741334
J Neuropathol Exp Neurol. 2003 Apr;62(4):329-39
12722825
Neurol Res. 2004 Dec;26(8):835-45
15727267
J Neurosci. 2000 Aug 15;20(16):6063-76
10934256
Neuroscience. 2002;115(1):125-36
12401327
FEBS Lett. 2000 Jul 7;476(3):118-23
10913597
Neurobiol Dis. 2002 Aug;10(3):289-05
12270691
J Immunol Methods. 2002 Jul 1;265(1-2):97-110
12072181
Trends Cell Biol. 2006 May;16(5):264-72
16621561
J Comp Neurol. 2000 Aug 28;424(3):409-38
10906711
ILAR J. 2003;44(2):85-95
12652003
Stroke. 1997 Apr;28(4):866-72
9099209
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5752-7
10318956