title: Construction and characterization of a novel staphylokinase variant with thrombin-inhibitory activity.
abstract: Staphylokinase (SAK) is an effective thrombolysis agent for therapy of myocardial infarction. We have constructed a fusion SAK variant (SAK-HV) with a thrombin-binding domain composed of 12 amino acids from hirudin and expressed it in Escherichia coli and purified the resultant protein. SAK-HV maintained fibrinolytic activity similar to SAK and had anticoagulant activity attributable to its hirudin segment. Measurement of thrombin-binding activity in vitro demonstrated that SAK-HV possessed binding activity with thrombin while SAK did not. SAK-HV might thus be a more potent thrombolytic agent with anticoagulation property than SAK.
authors.affiliation: Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Minji
authors.initials: M
authors.lastname: Zou
authors.forename: Shen
authors.initials: S
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Cai
authors.forename: Chen
authors.initials: C
authors.lastname: Wu
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Wang
authors.forename: Donggang
authors.initials: D
authors.lastname: Xu
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006629:Hirudins / Q000235:genetics* / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013917:Thrombin / Q000037:antagonists & inhibitors*
chemical_list: D006629:Hirudins; D011993:Recombinant Fusion Proteins; D013917:Thrombin; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus