title: DNA sensor development based on multi-wall carbon nanotubes for label-free influenza virus (type A) detection.
abstract: This paper describes the DNA immobilization using carbon multi-walled nanotubes (MWCNTs) for direct and label-free detection of influenza virus (type A). The DNA probe was attached on the sensor surface by means of covalent bonding between the amine and phosphate groups of the DNA sequence. The interaction between the DNA probe and the MWCNTs were characterized by Fourier Transform Infrared (FTIR) spectrometry, Raman spectra. The hybridization of the DNA probe and the target DNA were detected by changes in the conductance on the surface of sensors leading to the change in the output signal of the system. The results show that the DNA sensor can detect as low as 0.5 nM of the target DNA samples; the response time of DNA sensor is approximately 4 min.
authors.affiliation: Hanoi Advanced School of Science and Technology, Hanoi University of Technology, Vietnam. tampd-hast@mail.hut.edu.vn
authors.forename: Phuong Dinh
authors.initials: PD
authors.lastname: Tam
authors.forename: Nguyen
authors.initials: N
authors.lastname: Van Hieu
authors.forename: Nguyen Duc
authors.initials: ND
authors.lastname: Chien
authors.forename: Anh-Tuan
authors.initials: AT
authors.lastname: Le
authors.forename: Mai
authors.initials: M
authors.lastname: Anh Tuan
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D009980:Influenza A virus / Q000737:chemistry* / Q000302:isolation & purification*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors
chemical_list: D015342:DNA Probes; D037742:Nanotubes, Carbon; D004247:DNA