title: A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis.
abstract: Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing technique permits to differentiate Y. pestis isolates and turned out to be robust, reproducible, and cheap.
authors.affiliation: Army Medical Research Center, 00184 Rome, Italy. andrea.ciammaruconi@gmail.com
authors.forename: Andrea
authors.initials: A
authors.lastname: Ciammaruconi
authors.forename: Saverio
authors.initials: S
authors.lastname: Grassi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Faggioni
authors.forename: Riccardo
authors.initials: R
authors.lastname: De Santis
authors.forename: Valentina
authors.initials: V
authors.lastname: Pittiglio
authors.forename: Raffaele
authors.initials: R
authors.lastname: D'Amelio
authors.forename: Gilles
authors.initials: G
authors.lastname: Vergnaud
authors.forename: Florigio
authors.initials: F
authors.lastname: Lista
mesh_terms: D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D044366:Transition Temperature; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial