[Influence of electroacupuncture on hepatic cytochrome P450 1 A 1 expression and lipid peroxidation in nonalcoholic fatty liver rats].
OBJECTIVE
To study the effect of electroacupuncture (EA) on liver cytochrome P450 1 A 1 immunoactivity, superoxide dismutase (SOD) and malondialdehyde (MDA) contents in nonalcoholic fatty liver disease (NAFLD) rats.


METHODS
Thirty male SD rats were randomized into normal control (n = 10), model (n = 10) and EA (n = 10) groups. NAFLD model was established by feeding the animal with high-fat forage for 8 weeks. EA (1.6-2 Hz, 1-4 mA) was applied to bilateral "Zusanli" (ST 36), "Fenglong" (ST 40), "Sanyinjiao" (SP 6) and "Taichong" (LR 3) for 15 min, once daily for 4 weeks. Then the rats anesthetized with ether were killed for collecting liver tissue. Following homogenate and centrifugalization of the partial liver tissue, the supernatant was collected for assaying superoxide dismutase (SOD) and malondialdehyde (MDA) contents by xanthinoxidase chromometry and thio-malonylurea chromometry respectively. The other partial liver tissue samples were fixed in 10% formalin, followed by paraffin imbedding and sectioning (4 microm), and staining with streptavidin-perosidase methods respectively for displaying hepatic pathological changes and cytochrom P450 1 A 1 immunoreaction.


RESULTS
Compared with control group, hepatic SOD content of model group was significantly lower (P < 0.05), and MDA level and cytochrome P450 1 A 1 (CYP 1 A 1) integrated optic density (IOD) value were obviously higher in model group (P < 0.05). In comparison with model group, liver SOD level of EA group increased considerably (P < 0.05), while MDA level and CYP 1 A 1 IOD of EA group decreased evidently in EA group (P < 0.05).


CONCLUSIONS
EA can effectively reduce lipid peroxidation and up-regulate CYP 1 A 1 expression in nonalcoholic fatty liver tissue, which may contribute to its effect in improving fatty liver.
Feng
Wen-Qi
WQ
School of Chinese Medicine, Chongqing Medical University, Chongqing 400050, China. moonly1981@163.com
Liu
Qun-Ying
QY
Zeng
Zhi-Hua
ZH
Zhou
Lian-Shi
LS
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis*; D015671:Electroacupuncture; D005234:Fatty Liver / Q000378:metabolism / Q000628:therapy*; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D008315:Malondialdehyde; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase