Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.
A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.
Balk
Melike
M
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands. M.Balk@nioo.knaw.nl
Heilig
Hans G H J
HG
van Eekert
Miriam H A
MH
Stams
Alfons J M
AJ
Rijpstra
Irene C
IC
Sinninghe-Damsté
Jaap S
JS
de Vos
Willem M
WM
Kengen
Servé W M
SW
D001482:Base Composition; D002248:Carbon Monoxide / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005285:Fermentation; D019015:Geologic Sediments / Q000382:microbiology*; D045482:Hot Springs / Q000382:microbiology*; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D046968:Thermoanaerobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014421:Turkey
D004269:DNA, Bacterial; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002248:Carbon Monoxide
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