title: Molecular characterization of wheat germplasm using microsatellite markers.
abstract: We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discriminated the accessions and varieties into different clusters; genetic diversity was the highest between variety Kohistan-97 and accession number 011512, giving a genetic similarity value of 0.4198. Accession numbers 011484 and 011356 gave a genetic similarity value of 0.9589, indicating that these accessions were 95.89% similar. We found that microsatellite markers could characterize and discriminate all of the genotypes; more primers could be used for saturation of different regions in further studies.
authors.affiliation: Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan. siddraijaz_11@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Ijaz
authors.forename: I A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D014908:Triticum / Q000166:cytology* / Q000235:genetics*