Airway remodeling in a mouse asthma model assessed by in-vivo respiratory-gated micro-computed tomography.
The aim of our study was to evaluate the feasibility of non-invasive respiratory-gated micro-computed tomography (micro-CT) for assessment of airway remodelling in a mouse asthma model. Six female BALB/c mice were challenged intranasally with ovalbumin. A control group of six mice received saline inhalation. All mice underwent plethysmographic study and micro-CT. For each mouse, peribronchial attenuation values of 12 bronchi were measured, from which a peribronchial density index (PBDI) was computed. Mice were then sacrificed and lungs examined histologically. Final analysis involved 10 out of 12 mice. Agreement of measurements across observers and over time was very good (intraclass correlation coefficients: 0.94-0.98). There was a significant difference in PBDI between asthmatic and control mice (-210 vs. -338.9 HU, P = 0.008). PBDI values were correlated to bronchial muscle area (r = 0.72, P = 0.018). This study shows that respiratory-gated micro-CT may allow non-invasive monitoring of bronchial remodelling in asthmatic mice and evaluation of innovative treatment effects.
Lederlin
Mathieu
M
Laboratoire de Physiologie Cellulaire Respiratoire, Université Bordeaux 2, Bordeaux, France.
Ozier
Annaïg
A
Montaudon
Michel
M
Begueret
Hugues
H
Ousova
Olga
O
Marthan
Roger
R
Berger
Patrick
P
Laurent
François
F
D056151:Airway Remodeling; D000818:Animals; D001249:Asthma / Q000000981:diagnostic imaging*; D004195:Disease Models, Animal; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008168:Lung / Q000000981:diagnostic imaging*; D051379:Mice; D008807:Mice, Inbred BALB C; D011856:Radiographic Image Enhancement / Q000379:methods*; D015203:Reproducibility of Results; D055425:Respiratory-Gated Imaging Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014057:Tomography, X-Ray Computed / Q000379:methods*
Am J Respir Crit Care Med. 2000 May;161(5):1720-45
10806180
Radiology. 2004 Oct;233(1):165-71
15317950
Eur Radiol. 2008 Jul;18(7):1375-84
18431578
Eur J Anaesthesiol. 2005 Oct;22(10):785-94
16211751
Phys Med Biol. 2009 Apr 7;54(7):2121-30
19287083
Radiology. 2005 Sep;236(3):1053-8
16118177
Am J Respir Crit Care Med. 2001 Nov 15;164(10 Pt 2):S28-38
11734464
J Allergy Clin Immunol. 2005 Sep;116(3):544-9
16159622
Thorax. 2007 Jan;62(1):8-15
17189531
Med Phys. 2005 Sep;32(9):2888-98
16266103
Lancet. 1986 Feb 8;1(8476):307-10
2868172
Clin Exp Allergy. 2005 Oct;35(10):1251-3
16238782
J Exp Med. 2007 Dec 24;204(13):3173-81
18056286
Am J Respir Crit Care Med. 2002 Jan 1;165(1):108-16
11779739
N Engl J Med. 2007 Mar 29;356(13):1327-37
17392302
Thorax. 2002 Mar;57(3):247-53
11867830
Eur Respir J. 1999 Dec;14(6):1394-402
10624773
Thorax. 2002 Mar;57(3):242-6
11867829
Am J Physiol Lung Cell Mol Physiol. 2001 Aug;281(2):L394-402
11435214
Int J Radiat Oncol Biol Phys. 1995 Jul 30;32(5):1359-70
7635776
Med Phys. 2006 Jan;33(1):216-24
16485428
Med Phys. 2003 Nov;30(11):2869-77
14655933
Toxicol Pathol. 2007 Jan;35(1):59-64
17325973
Neoplasia. 2005 Aug;7(8):730-40
16207475
Lab Invest. 1999 Dec;79(12):1559-71
10616206
BMJ. 1996 Sep 21;313(7059):744
8819450
BMJ. 1996 Jul 6;313(7048):41-2
8664775
Am J Respir Crit Care Med. 2000 Oct;162(4 Pt 1):1518-23
11029371
J Appl Physiol (1985). 2004 Jul;97(1):286-92
15033965
Invest Radiol. 2008 Jun;43(6):453-60
18496052
Allergol Int. 2007 Dec;56(4):341-8
17965577
Invest Radiol. 2005 May;40(5):263-9
15829823
Eur Radiol. 1996;6(2):199-206
8797980