The functional relationships underlying a synthetic genetic network.
Here, we focus on synthetic lethal genetic interactions, examples of genetic enhancements, where mutations in two different genes result in lethality but only when present together. We recently identified the synthetic lethal network around the PKC1 gene encoding the essential protein kinase C of yeast. We found that this network is heavily enriched for interactions with genes whose products are closely linked to Pkc1 signaling in vivo. Here, we show that: the PKC1 gene elicits a distinct spectrum of genetic interactions to SLT2, encoding a non-essential component of the very same signaling pathway. We also show that the terminal phenotype underlying the synthetic lethal network around PKC1 is not uniform. Synthetic lethal genetic networks thus appear to be very heterogeneous in nature with important implications for what functional relationships can be discovered from them.
Krause
Sue A
SA
Molecular Genetics and Integrative & Systems Biology; Faculty of Biomedical and Life Sciences; University of Glasgow; Glasgow, Scotland UK.
Gray
Joseph V
JV
Trends Genet. 1993 Oct;9(10):362-6
8273152
Cold Spring Harb Symp Quant Biol. 2006;71:141-8
17381290
Differentiation. 2004 Mar;72(2-3):81-91
15066188
Immunol Cell Biol. 2005 Jun;83(3):217-23
15877598
Trends Genet. 2006 Jan;22(1):56-63
16309778
Curr Biol. 2002 Apr 2;12(7):588-93
11937029
Science. 2001 Feb 9;291(5506):1001-4
11232561
Science. 2004 Feb 6;303(5659):808-13
14764870
J Biol. 2006;5(4):11
16762047
Eukaryot Cell. 2008 Nov;7(11):1880-7
18806213
Science. 2001 Dec 14;294(5550):2364-8
11743205
Mol Cell. 2004 Nov 5;16(3):487-96
15525520
Nat Genet. 2005 Oct;37(10):1147-52
16155567
Microbiol Mol Biol Rev. 2005 Jun;69(2):262-91
15944456