[Effects of resveratrol on nuclear factor-kappaB and pro-inflammatory factors from lipopolysaccharide activated murine peritoneal macrophages].
OBJECTIVE
To investigate activation of nuclear factor-kappaB (NF-kappaB) and the expression of inflammatory cytokines in lipopolysaccharide-induced murine peritoneal macrophages stimulated with resveratrol (Res).


METHODS
Peritoneal macrophages cultured in vitro were given with 1 mg/L LPS or 25 mmol/L Res + 1 mg/L LPS. The NF-kappaB activity of peritoneal macrophages was tested by electrophoretic mobility shift assay (EMSA). The expression of TNF-alpha, IL-beta, IL-6 in peritoneal macrophages were measured by reverse transcription polymerase chain reaction techniques (RT-PCR) and enzyme-linked immuno sorbent assay (ELISA).


RESULTS
The results showed that the activity of NF-kappaB and the level of TNF-alpha, IL-1beta, IL-6 significantly increased from 6 to 12 hour after LPS stimulation (P < 0.001). Compared with LPS-stimulated group, both NF-kappaB activity and concentration of TNF-alpha, IL-1beta, IL-6 were significantly lowered in Res + LPS group (P < 0.005).


CONCLUSIONS
The results suggested LPS might activate NF-kappaB in peritoneal macrophages and induce the increase of transcription and expression of TNF-alpha, IL-1beta, IL-6 genes; Res could inhibit the activation of NF-kappaB and regulate the expression of TNF-alpha, IL-1beta and IL-6 genes in peritoneal macrophages.
Zhang
Shen
S
Department of Laboratory Medicine, Huaihua Medical College, Huaihua 418000, China. zhang123shen@163.com
Li
Shuping
S
Wei
Taotao
T
D000818:Animals; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D000077185:Resveratrol