title: Arabidopsis actin-related protein ARP5 in multicellular development and DNA repair.
abstract: Actin-related protein 5 (ARP5) is a conserved subunit of the INO80 chromatin-remodeling complex in yeast and mammals. We have characterized the expression and subcellular distribution of Arabidopsis thaliana ARP5 and explored its role in the epigenetic control of multicellular development and DNA repair. ARP5-specific monoclonal antibodies localized ARP5 protein to the nucleoplasm of interphase cells in Arabidopsis and Nicotiana tabacum. ARP5 promoter-reporter fusions and the ARP5 protein are ubiquitously expressed. A null mutant and a severe knockdown allele produced moderately dwarfed plants with all organs smaller than the wild type. The small and slightly deformed organs such as leaves and hypocotyls were composed of small-sized cells. The ratio of leaf stomata to epidermal cells was high in the mutant, which also exhibited a delayed stomatal development compared with the wild type. Mutant plants were hypersensitive to DNA-damaging reagents including hydroxyurea, methylmethane sulfonate, and bleocin, demonstrating a role for ARP5 in DNA repair. Interestingly, the hypersensitivity phenotype of ARP5 null allele arp5-1 is stronger than the severe knockdown allele arp5-2. Moreover, a wild-type transgene fully complemented all developmental and DNA repair mutant phenotypes. Despite the common participation of both ARP4 and ARP5 in the INO80 complex, ARP4- and ARP5-deficient plants displayed only a small subset of common phenotypes and each displayed novel phenotypes, suggesting that in Arabidopsis they have both shared and unique functions.
authors.affiliation: Department of Genetics, Davison Life Sciences Complex, University of Georgia, Athens, GA 30602, USA.
authors.forename: Muthugapatti K
authors.initials: MK
authors.lastname: Kandasamy
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: McKinney
authors.forename: Roger B
authors.initials: RB
authors.lastname: Deal
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Smith
authors.forename: Richard B
authors.initials: RB
authors.lastname: Meagher
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001761:Bleomycin / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D006918:Hydroxyurea / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009153:Mutagens / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010641:Phenotype; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism; D019076:Transgenes
chemical_list: C512743:ATARP5 protein, Arabidopsis; D000903:Antibiotics, Antineoplastic; D029681:Arabidopsis Proteins; D008840:Microfilament Proteins; D009153:Mutagens; D019384:Nucleic Acid Synthesis Inhibitors; D011993:Recombinant Fusion Proteins; D001761:Bleomycin; D008741:Methyl Methanesulfonate; D006918:Hydroxyurea
references.citation: Mol Cell. 2004 Nov 5;16(3):465-77
references.pmid: 15525518
references.citation: Mol Biol Cell. 2005 Dec;16(12):5736-48
references.pmid: 16195354
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12406-11
references.pmid: 8901594
references.citation: Plant J. 2002 Aug;31(4):517-28
references.pmid: 12182708
references.citation: Nat Struct Mol Biol. 2008 May;15(5):469-76
references.pmid: 18408732
references.citation: Science. 2004 Jan 16;303(5656):343-8
references.pmid: 14645854
references.citation: Int Rev Cell Mol Biol. 2009;277:157-215
references.pmid: 19766970
references.citation: Plant Mol Biol. 1994 Sep;25(6):989-94
references.pmid: 7919218
references.citation: Genes Dev. 1996 Mar 1;10(5):604-19
references.pmid: 8598290
references.citation: Plant J. 2005 Mar;41(6):845-58
references.pmid: 15743449
references.citation: Plant Physiol. 2005 Aug;138(4):2019-32
references.pmid: 16040647
references.citation: Plant Physiol. 2005 Dec;139(4):1576-85
references.pmid: 16339804
references.citation: Nature. 1996 Jun 6;381(6582):529-31
references.pmid: 8632827
references.citation: Plant Cell. 2005 Oct;17(10):2633-46
references.pmid: 16141450
references.citation: Plant Cell. 2007 Jan;19(1):74-83
references.pmid: 17220196
references.citation: Plant J. 2003 Mar;33(5):939-48
references.pmid: 12609034
references.citation: Genetics. 2006 Jun;173(2):985-94
references.pmid: 16547115
references.citation: Annu Rev Biochem. 2002;71:755-81
references.pmid: 12045110
references.citation: Trends Biochem Sci. 2009 Feb;34(2):71-7
references.pmid: 19062292
references.citation: Trends Cell Biol. 2007 Jul;17(7):325-32
references.pmid: 17643304
references.citation: J Biol Chem. 2004 Jan 2;279(1):223-30
references.pmid: 14573610
references.citation: Trends Cell Biol. 2004 Aug;14(8):435-42
references.pmid: 15308210
references.citation: J Immunol Methods. 2009 Mar 31;343(1):28-41
references.pmid: 19187782
references.citation: Nucleic Acids Res. 1995 Jan 11;23(1):130-7
references.pmid: 7870578
references.citation: Mol Cell. 2004 Nov 5;16(3):479-85
references.pmid: 15525519
references.citation: Trends Plant Sci. 2003 Feb;8(2):90-5
references.pmid: 12597876
references.citation: Curr Biol. 2008 Apr 22;18(8):566-75
references.pmid: 18406137
references.citation: Plant Cell. 2003 Jul;15(7):1671-82
references.pmid: 12837955
references.citation: J Biol Chem. 2003 Jan 10;278(2):823-8
references.pmid: 12403775
references.citation: Cell. 2004 Dec 17;119(6):777-88
references.pmid: 15607975
references.citation: Mutat Res. 1999 Aug 11;429(1):13-26
references.pmid: 10434021
references.citation: Plant Physiol. 2004 Sep;136(1):2621-32
references.pmid: 15375207
references.citation: Trends Plant Sci. 2004 Apr;9(4):196-202
references.pmid: 15063870
references.citation: Nat Struct Mol Biol. 2008 Apr;15(4):338-45
references.pmid: 18376411
references.citation: Exp Cell Res. 2009 Jan 15;315(2):206-17
references.pmid: 19014934
references.citation: EMBO J. 1987 Dec 20;6(13):3901-7
references.pmid: 3327686
references.citation: Mol Cell. 2003 Jul;12(1):147-55
references.pmid: 12887900
references.citation: Biochem Biophys Res Commun. 2008 Sep 5;373(4):602-6
references.pmid: 18593569
references.citation: Plant J. 2007 Nov;52(3):460-72
references.pmid: 17877706
references.citation: Mutat Res. 2004 Oct 4;554(1-2):185-92
references.pmid: 15450417
references.citation: Plant Cell Physiol. 2008 May;49(5):858-63
references.pmid: 18385164