Improvement of alpha-L: -arabinofuranosidase production by Talaromyces thermophilus and agro-industrial residues saccharification.
This study is an application of an experimental design methodology for the optimization of the culture conditions of alpha-L: -arabinofuranosidase production by Talaromyces thermophilus. Wheat bran and yeast extract were first selected as the best carbon and nitrogen sources, respectively, for enzyme production. A Plackett-Burman design was then used to evaluate the effects of eight variables. Statistical analyses showed that while pH had a negative effect on alpha-L: -arabinofuranosidase production, wheat bran and MgSO(4) had a significantly positive effect. The values of the latter three parameters were further optimised using a central composite design and a response surface methodology. The experimental results were fitted to a second-order polynomial model that yielded a determination coefficient of R(2) = 0.91. The statistical output showed that the linear and quadric terms of the three variables had significant effects. Using optimal conditions, the experimental value of alpha-L: -arabinofuranosidase activity produced was very close to the model-predicted value. The optimal temperature and pH of enzyme activity were 55 degrees C and 7.0, respectively. This enzyme was very stable over a considerable pH range from 4 to 9. The crude enzyme of T. thermophilus rich in alpha-L: -arabinofuranosidase was also used for saccharification of lignocellulosic materials and arabinose production.
Guerfali
Mohamed
M
Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, BP 1177, 3038 Sfax, Tunisia.
Chaabouni
Moncef
M
Gargouri
Ali
A
Belghith
Hafedh
H
D001089:Arabinose / Q000378:metabolism; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004043:Dietary Fiber; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D004795:Enzyme Stability; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D008278:Magnesium Sulfate / Q000378:metabolism; D008954:Models, Biological; D015233:Models, Statistical; D009584:Nitrogen / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D032901:Talaromyces / Q000201:enzymology*; D013696:Temperature
D003470:Culture Media; D004043:Dietary Fiber; D005656:Fungal Proteins; D010940:Plant Proteins; D011134:Polysaccharides; D002244:Carbon; D008278:Magnesium Sulfate; C007916:hemicellulose; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen