An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting.
Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic (DEP) microfluidic platform based on planar electrodes that form three-dimensional (3D) DEP gates. This platform can continuously perform these tasks with a throughput of 3 muLmin. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100 mul of a heterogeneous colony of 10(7) colony forming units ml was processed in a single pass within 30 min.
Cheng
I-Fang
IF
Institute of Nanotechnology and Microsystem Engineering, Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China.
Chang
Hsien-Chang
HC
Hou
Diana
D
Chang
Hsueh-Chia
HC
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