Immunoproteomic analysis of outer membrane proteins and extracellular proteins of Actinobacillus pleuropneumoniae JL03 serotype 3.
BACKGROUND
Actinobacillus pleuropneumoniae is the causative agent of porcine contagious pleuropneumonia, a highly contagious respiratory infection in pigs, and all the 15 serotypes are able to cause disease. Current vaccines including subunit vaccines could not provide satisfactory protection against A. pleuropneumoniae. In this study, the immunoproteomic approach was applied to the analysis of extracellular and outer membrane proteins of A. pleuropneumoniae JL03 serotype 3 for the identification of novel immunogenic proteins for A. pleuropneumoniae.


RESULTS
A total of 30 immunogenic proteins were identified from outer membrane and extracellular proteins of JL03 serotype 3, of which 6 were known antigens and 24 were novel immunogenic proteins for A. pleuropneumoniae.


CONCLUSIONS
These data provide information about novel immunogenic proteins for A. pleuropneumoniae serotype 3, and are expected to aid in development of novel vaccines against A. pleuropneumoniae.
Liao
Yonghong
Y
College of Veterinary Medicine, Huazhong Agricultural University, Hubei, PR China. ebrain04@163.com
Deng
Junhua
J
Zhang
Anding
A
Zhou
Mingguang
M
Hu
Yong
Y
Chen
Huanchun
H
Jin
Meilin
M
D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D020543:Proteome / Q000276:immunology / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D020543:Proteome
Can J Microbiol. 2008 Sep;54(9):734-41
18772936
Vet Microbiol. 1996 Apr;49(3-4):159-68
8734634
PLoS One. 2008 Jan 16;3(1):e1450
18197260
Infect Immun. 2003 Aug;71(8):4563-79
12874336
J Cell Biol. 2004 Jan 5;164(1):19-24
14699090
Infect Genet Evol. 2008 Dec;8(6):772-6
18692158
Protein Sci. 2004 Jan;13(1):89-99
14691225
Vet Microbiol. 1997 Nov;58(2-4):239-49
9453134
Infect Immun. 2006 Oct;74(10):5888-92
16988268
Infect Immun. 1993 May;61(5):1793-8
8478068
Microb Pathog. 2000 Nov;29(5):279-87
11031122
Anim Health Res Rev. 2008 Jun;9(1):25-45
18346296
BMC Genomics. 2007 Mar 13;8:72
17355629
Infect Immun. 1997 Sep;65(9):3701-7
9284140
Infect Immun. 2008 Jun;76(6):2284-95
18378638
Electrophoresis. 1998 May;19(5):837-44
9629924
J Bacteriol. 2001 Feb;183(4):1242-7
11157936
Infect Immun. 2000 Feb;68(2):796-800
10639448
Electrophoresis. 2000 Apr;21(6):1037-53
10786879
Appl Environ Microbiol. 2006 Oct;72(10):6623-31
17021213
Anal Biochem. 1995 Jul 20;229(1):140-3
8533884
Microb Pathog. 2007 Nov-Dec;43(5-6):189-97
17689221
FEMS Microbiol Lett. 2000 Oct 1;191(1):109-13
11004407
Vaccine. 2009 Jan 22;27(4):542-8
19028538
Microbiology (Reading). 2009 Mar;155(Pt 3):979-988
19246768
Infect Immun. 1996 Jan;64(1):83-90
8557378
Infect Immun. 2001 Jul;69(7):4438-46
11401984
Vaccine. 2007 Mar 8;25(12):2261-8
17239499
Proteome Sci. 2007 Jul 27;5:11
17662140
Proteomics. 2006 Feb;6(3):836-44
16372264
Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):913-9
15983414
Electrophoresis. 1999 Aug;20(11):2269-79
10493131
Proteins. 2004 May 1;55(2):479-81
15048838
Vaccine. 2003 Sep 8;21(25-26):3601-7
12922088
Proteomics. 2007 Jun;7(11):1854-65
17476711
Infect Immun. 2006 Mar;74(3):1725-40
16495545
J Gen Microbiol. 1993 Aug;139(8):1723-8
8409915
Proteomics. 2008 Sep;8(17):3506-15
18686301
Proteomics. 2006 Jul;6(13):3767-80
16739129
Microb Pathog. 1994 Jul;17(1):23-36
7861951
Science. 2003 Jan 10;299(5604):262-5
12522254
Vaccine. 1994 May;12(6):535-41
7518627
J Vet Med Sci. 2004 Sep;66(9):1059-63
15472468
Proteomics. 2007 Sep;7(17):3143-53
17787032
J Biochem. 2006 Feb;139(2):213-21
16452309
Anal Chem. 2006 Jan 1;78(1):199-206
16383328
Infect Immun. 2003 Jul;71(7):3960-70
12819083
Antimicrob Agents Chemother. 2005 Sep;49(9):3922-5
16127072
Res Vet Sci. 2001 Jun;70(3):255-6
11676623
Eur J Biochem. 2000 May;267(10):2871-81
10806384
Electrophoresis. 1996 Jan;17(1):185-90
8907538
Proteomics. 2005 Dec;5(18):4777-93
16281178
Infect Immun. 1996 Dec;64(12):5255-62
8945574
BMC Genomics. 2009 Feb 24;10:88
19239696
J Bacteriol. 1996 Dec;178(23):6952-9
8955319