title: Molybdenum cofactors, enzymes and pathways.
abstract: The trace element molybdenum is essential for nearly all organisms and forms the catalytic centre of a large variety of enzymes such as nitrogenase, nitrate reductases, sulphite oxidase and xanthine oxidoreductases. Nature has developed two scaffolds holding molybdenum in place, the iron-molybdenum cofactor and pterin-based molybdenum cofactors. Despite the different structures and functions of molybdenum-dependent enzymes, there are important similarities, which we highlight here. The biosynthetic pathways leading to both types of cofactor have common mechanistic aspects relating to scaffold formation, metal activation and cofactor insertion into apoenzymes, and have served as an evolutionary 'toolbox' to mediate additional cellular functions in eukaryotic metabolism.
authors.affiliation: Institute of Biochemistry, Department of Chemistry & Centre for Molecular Medicine, University of Cologne, 47 Zuelpicher Street, 50674 Cologne, Germany. gschwarz@uni-koeln.de
authors.forename: Günter
authors.initials: G
authors.lastname: Schwarz
authors.forename: Ralf R
authors.initials: RR
authors.lastname: Mendel
authors.forename: Markus W
authors.initials: MW
authors.lastname: Ribbe
mesh_terms: D000818:Animals; D003067:Coenzymes / Q000172:deficiency / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism; D008667:Metalloproteins / Q000172:deficiency / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D009711:Nucleotides / Q000378:metabolism; D011621:Pteridines / Q000378:metabolism*; D011622:Pterins / Q000378:metabolism
chemical_list: D003067:Coenzymes; D003300:Copper; D004798:Enzymes; D007501:Iron; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009711:Nucleotides; D011621:Pteridines; D011622:Pterins; C022261:molybdopterin
references.citation: Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12908-13
references.pmid: 11050171
references.citation: Nature. 1997 May 22;387(6631):370-6
references.pmid: 9163420
references.citation: Structure. 2003 Oct;11(10):1251-63
references.pmid: 14527393
references.citation: Plant Cell. 2005 Apr;17(4):1167-79
references.pmid: 15772287
references.citation: Cell Mol Life Sci. 2005 Dec;62(23):2792-810
references.pmid: 16261263
references.citation: Biochim Biophys Acta. 2007 May;1774(5):527-39
references.pmid: 17459792
references.citation: Am J Hum Genet. 2001 Jan;68(1):208-13
references.pmid: 11095995
references.citation: J Biol Chem. 2001 Nov 2;276(44):40381-4
references.pmid: 11553608
references.citation: J Biol Chem. 1995 Jan 20;270(3):1082-7
references.pmid: 7836363
references.citation: J Biol Chem. 1992 May 25;267(15):10199-202
references.pmid: 1587808
references.citation: J Biol Chem. 2001 Jun 22;276(25):22024-31
references.pmid: 11290749
references.citation: J Biol Chem. 1995 Nov 10;270(45):26890-6
references.pmid: 7592933
references.citation: J Biol Chem. 2004 Apr 16;279(16):15994-9
references.pmid: 14761975
references.citation: Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10723-8
references.pmid: 11005854
references.citation: J Biol Chem. 2005 Jul 1;280(26):24999-5007
references.pmid: 15863498
references.citation: J Bacteriol. 1984 Apr;158(1):187-94
references.pmid: 6370956
references.citation: Nature. 2004 Aug 12;430(7001):803-6
references.pmid: 15306815
references.citation: Trends Mol Med. 2001 Feb;7(2):64-9
references.pmid: 11286757
references.citation: Nature. 2001 Nov 15;414(6861):325-9
references.pmid: 11713534
references.citation: J Inorg Biochem. 2007 Nov;101(11-12):1630-41
references.pmid: 17521738
references.citation: J Biol Chem. 2003 Mar 28;278(13):10885-90
references.pmid: 12519777
references.citation: Toxicology. 1986 Dec 15;42(2-3):99-109
references.pmid: 3541288
references.citation: Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5297-301
references.pmid: 16567617
references.citation: Drug Discov Today. 2005 Aug 15;10(16):1103-9
references.pmid: 16182195
references.citation: Arch Biochem Biophys. 2005 Jan 1;433(1):107-16
references.pmid: 15581570
references.citation: J Bacteriol. 1997 Sep;179(18):5963-6
references.pmid: 9294461
references.citation: Chem Rev. 1996 Nov 7;96(7):3013-3030
references.pmid: 11848850
references.citation: J Biol Chem. 2006 Oct 13;281(41):30534-41
references.pmid: 16893897
references.citation: J Biol Chem. 2006 Nov 17;281(46):34796-802
references.pmid: 16973608
references.citation: Nat Struct Biol. 2001 Jan;8(1):42-6
references.pmid: 11135669
references.citation: Biochemistry. 2005 Oct 4;44(39):12955-69
references.pmid: 16185064
references.citation: Chem Rev. 1996 Nov 7;96(7):2983-3012
references.pmid: 11848849
references.citation: J Biol Chem. 2006 Oct 6;281(40):30186-94
references.pmid: 16873364
references.citation: Science. 2002 Apr 12;296(5566):352-6
references.pmid: 11951047
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11679-84
references.pmid: 18697927
references.citation: J Biol Chem. 2003 Apr 18;278(16):14523-32
references.pmid: 12571226
references.citation: Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17119-24
references.pmid: 17050696
references.citation: J Biol Chem. 2001 Dec 14;276(50):46989-94
references.pmid: 11598126
references.citation: Science. 1992 Sep 18;257(5077):1653-9
references.pmid: 1529353
references.citation: Biochim Biophys Acta. 2008 Sep;1778(9):1897-929
references.pmid: 17964535
references.citation: Trends Biochem Sci. 2002 Jul;27(7):360-7
references.pmid: 12114025
references.citation: Biochemistry. 2008 Jan 15;47(2):615-26
references.pmid: 18092812
references.citation: J Biol Chem. 2007 Jul 20;282(29):21561-71
references.pmid: 17517886
references.citation: Chem Rev. 2004 Feb;104(2):1159-73
references.pmid: 14871152
references.citation: Biochemistry. 2008 Jan 22;47(3):949-56
references.pmid: 18154309
references.citation: J Biol Inorg Chem. 2007 May;12(4):449-60
references.pmid: 17203313
references.citation: J Biol Inorg Chem. 2009 Jun;14(5):663-72
references.pmid: 19234723
references.citation: Met Ions Biol Syst. 2002;39:31-74
references.pmid: 11913129
references.citation: Hum Mutat. 2003 Jun;21(6):569-76
references.pmid: 12754701
references.citation: Structure. 2000 Nov 15;8(11):1115-25
references.pmid: 11080634
references.citation: Cell. 2008 Jul 25;134(2):268-78
references.pmid: 18662542
references.citation: Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17125-30
references.pmid: 17062756
references.citation: J Mol Biol. 2008 Jun 13;379(4):881-99
references.pmid: 18485362
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):20126-30
references.pmid: 18077439
references.citation: J Biol Inorg Chem. 2004 Oct;9(7):791-9
references.pmid: 15311335
references.citation: Biochemistry. 2009 Mar 24;48(11):2310-20
references.pmid: 19206188
references.citation: Nature. 2007 Mar 8;446(7132):213-6
references.pmid: 17322901
references.citation: J Biol Chem. 2006 Jun 9;281(23):15701-8
references.pmid: 16597619
references.citation: J Biol Chem. 2004 Oct 8;279(41):43035-45
references.pmid: 15273247
references.citation: Biochem J. 2003 May 15;372(Pt 1):15-32
references.pmid: 12578558
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15971-6
references.pmid: 12475995
references.citation: Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18807-12
references.pmid: 18003916
references.citation: Hum Mol Genet. 2004 Jun 15;13(12):1249-55
references.pmid: 15115759
references.citation: Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1333-8
references.pmid: 9990024
references.citation: Science. 2002 Sep 6;297(5587):1696-700
references.pmid: 12215645
references.citation: Microbiology (Reading). 2007 Mar;153(Pt 3):633-651
references.pmid: 17322183
references.citation: Free Radic Biol Med. 2002 Sep 15;33(6):774-97
references.pmid: 12208366
references.citation: J Bacteriol. 2006 Sep;188(18):6498-505
references.pmid: 16952940
references.citation: Annu Rev Plant Biol. 2006;57:623-47
references.pmid: 16669776
references.citation: Annu Rev Biochem. 2005;74:247-81
references.pmid: 15952888
references.citation: J Biol Chem. 2005 Feb 11;280(6):4213-8
references.pmid: 15561708
references.citation: J Biol Chem. 2002 Apr 19;277(16):14299-305
references.pmid: 11823455
references.citation: FEBS J. 2009 May;276(10):2762-74
references.pmid: 19368556
references.citation: Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):5946-51
references.pmid: 15073332
references.citation: Pediatrics. 2005 Sep;116(3):757-66
references.pmid: 16140720
references.citation: J Biol Chem. 2001 Sep 28;276(39):36268-74
references.pmid: 11459846
references.citation: J Biol Chem. 2002 May 24;277(21):18303-12
references.pmid: 11891227
references.citation: J Biol Chem. 1993 Jun 25;268(18):13493-8
references.pmid: 8514781
references.citation: J Biol Chem. 2006 Mar 10;281(10):6884-8
references.pmid: 16407262
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:277-303
references.pmid: 15012211
references.citation: J Biol Chem. 2004 Oct 1;279(40):41398-403
references.pmid: 15247236
references.citation: Cell. 1997 Dec 26;91(7):973-83
references.pmid: 9428520
references.citation: Annu Rev Nutr. 1991;11:121-40
references.pmid: 1892697
references.citation: Structure. 2002 Jan;10(1):115-25
references.pmid: 11796116
references.citation: Proc Natl Acad Sci U S A. 2005 Mar 1;102(9):3236-41
references.pmid: 15728375
references.citation: Biochemistry. 2008 Apr 1;47(13):3973-81
references.pmid: 18314963
references.citation: Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1238-43
references.pmid: 16423898
references.citation: Am J Hum Genet. 1999 Mar;64(3):698-705
references.pmid: 10053003
references.citation: Hum Mol Genet. 2002 Dec 15;11(26):3309-17
references.pmid: 12471057
references.citation: Biochim Biophys Acta. 2006 Jul;1763(7):621-35
references.pmid: 16784786
references.citation: J Biol Chem. 2006 Jul 7;281(27):18343-50
references.pmid: 16636046
references.citation: FEBS Lett. 2005 Dec 19;579(30):6786-90
references.pmid: 16310786
references.citation: J Biol Chem. 2004 Dec 31;279(53):55241-6
references.pmid: 15504727
references.citation: Proc Natl Acad Sci U S A. 2006 May 2;103(18):6829-34
references.pmid: 16632608
references.citation: J Biol Chem. 2008 Apr 11;283(15):9642-50
references.pmid: 18258600
references.citation: PLoS Genet. 2008 Feb 29;4(2):e1000004
references.pmid: 18454190
references.citation: Nat Struct Biol. 2003 Sep;10(9):681-7
references.pmid: 12910261