Flow injection-capillary electrophoresis frontal analysis method for the study of the interactions of a series of drugs with human serum albumin.
In this paper, a fast method for the study on the interactions of a series of drugs used in the treatment of hypertension with human serum albumin (HSA) by flow injection-capillary electrophoresis (FI-CE) was developed based on the principle of frontal analysis (FA). The binding parameters were determined by FI-CE-FA from Scatchard equation and compared with results obtained by non-CE methods and literature values. A multiple linear regression (MLR) model between the drug-protein binding constants (K) and structural descriptors of drugs was constructed. L-tryptophan (L-try) and phenylbutazone (PB) were used as displacement reagents to investigate the binding sites of a series of drugs on HSA. The binding synergism effect between drugs and the effects of many metal ions existing in human plasma on protein binding were also investigated systematically.
Liu
Xiumei
X
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Li
Shuyan
S
Zhang
Jingshu
J
Chen
Xingguo
X
D000327:Adsorption; D004357:Drug Synergism; D019075:Electrophoresis, Capillary / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008670:Metals / Q000737:chemistry / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000378:metabolism; D021281:Quantitative Structure-Activity Relationship; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism
D008670:Metals; D004364:Pharmaceutical Preparations; D012709:Serum Albumin