Type I pro-collagen promoting and anti-collagenase activities of Phyllanthus emblica extract in mouse fibroblasts.
As part of an ongoing search for the novel pharmacological activities of Phyllanthus emblica, the present study has shown its type I collagen promoting and anti-collagenase effects on primary mouse fibroblast cells. At a concentration of 0.1 mg/ml, emblica extract significantly increased the type I pro-collagen level up to 1.65-fold, and 6.78-fold greater than that of an untreated control, determined by immunocytochemistry and Western blot analysis, respectively. Emblica extract caused an approximately 7.75-fold greater type I pro-collagen induction compared to the known herbal collagen enhancer asiaticoside at the same treatment concentration (0.1 mg/ml). Moreover, emblica extract inhibited collagenase activity in a dose-dependent manner. Maximal inhibition was observed (78.67 +/- 3.51%) at a concentration of 1 mg/ml. In summary, emblica extract has a promising pharmacological effect that benefits collagen synthesis and protects against its degradation and could be used as a natural anti-aging ingredient.
Chanvorachote
Pithi
P
Department of Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Pongrakhananon
Varisa
V
Luanpitpong
Sudjit
S
Chanvorachote
Boontarika
B
Wannachaiyasit
Sumalee
S
Nimmannit
Ubonthip
U
D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis*; D017364:Collagenases / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005638:Fruit / Q000378:metabolism; D007150:Immunohistochemistry; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D029842:Phyllanthus emblica / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D015595:Skin Aging / Q000187:drug effects
D024042:Collagen Type I; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D017364:Collagenases