title: Giardia intestinalis assemblages A and B infections in Nepal.
abstract: Giardia intestinalis is comprised of two major genotypes, A and B, which may vary in their propensity to cause disease. We tested for the presence of these two genotypes in stool samples from patients with gastrointestinal symptoms in Nepal. A total of 1,096 clinical specimens were screened by microscopy, and 45 samples with G. intestinalis were identified. Giardia infection was confirmed in 35 of 45 samples by a Giardia specific real-time polymerase chain reaction (PCR) assay. Genotyping of the Giardia PCR product by restriction fragment length polymorphism indicated that 74% (26 of 35) were assemblage B, 20% (7 of 35) were assemblage A, and 6% (2 of 35) were mixed assemblages.
authors.affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA. anjanas67@hotmail.com
authors.forename: Anjana
authors.initials: A
authors.lastname: Singh
authors.forename: Lalitha
authors.initials: L
authors.lastname: Janaki
authors.forename: William A
authors.initials: WA
authors.lastname: Petri
authors.forename: Eric R
authors.initials: ER
authors.lastname: Houpt
mesh_terms: D000818:Animals; D005243:Feces / Q000469:parasitology; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics; D005873:Giardiasis / Q000453:epidemiology* / Q000469:parasitology*; D006801:Humans; D009390:Nepal / Q000453:epidemiology; D016133:Polymerase Chain Reaction
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