title: Production of beta-maltooligosaccharides of alpha- and delta-tocopherols by Klebsiella pneumoniae and cyclodextrin glucanotransferase as anti-allergic agents.
abstract: The glycosylation of alpha- and delta-tocopherols using Klebsiella pneumoniae and cyclodextrin glucanotransferase (CGTase) was investigated. K. pneumoniae converted alpha- and delta-tocopherols into the corresponding beta-glucosides in 10 and 8% yield, respectively. CGTase glycosylated alpha-tocopheryl beta-glucoside to alpha-tocopheryl beta-maltoside (51%) and alpha-tocopheryl beta-maltotrioside (35%). On the other hand, delta-tocopheryl beta-glucoside was converted into the corresponding beta-maltoside (45%) and beta-maltotrioside (29%) by CGTase. The beta-glucoside of alpha-tocopherol, and beta-glucoside and beta-maltoside of delta-tocopherol showed inhibitory effects on IgE antibody production and on histamine release from rat peritoneal mast cells.
authors.affiliation: Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan. shimoda@med.oita-u.ac.jp
authors.forename: Kei
authors.initials: K
authors.lastname: Shimoda
authors.forename: Masaaki
authors.initials: M
authors.lastname: Akagi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hamada
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D005964:Glucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D006636:Histamine Release / Q000187:drug effects; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D024505:Tocopherols / Q000737:chemistry / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018926:Anti-Allergic Agents; D009844:Oligosaccharides; C021705:maltooligosaccharides; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D024502:alpha-Tocopherol; C479072:delta-tocopherol; D024505:Tocopherols
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