title: Inhibitor of DNA binding-1 promotes the migration and proliferation of endothelial progenitor cells in vitro.
abstract: Migration and proliferation of endothelial progenitor cells (EPCs) are the key mechanisms in re-endothelialization after vascular injury. Inhibitor of DNA binding-1 (Id1) function has been linked to the proliferation, migration, and senescence of cells, and studies have shed light on the relationship between Id1 and the biological functions of EPCs. On the basis of the available data concerning Id1 and the behavior of EPCs, we hypothesized that Id1 was an important regulator in modulating the migration and proliferation of EPCs. Culture of spleen-derived EPCs was done as previously described. Id1 was presented at low levels in EPCs. Id1 was localized predominantly in the cytoplasm, and was rapidly upregulated by stimulation with serum and vascular endothelial growth factor. The migration and proliferation of EPCs were extensively improved by overexpression of adenovirus-mediated exogenous Id1 and inhibited by silencing of endogenous Id1 in EPCs. These results suggest that Id1 has a direct role in regulation of the migration and proliferation in EPCs.
authors.affiliation: Department of Cardiology, Institute of Cardiovascular Science of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Rui-wei
authors.initials: RW
authors.lastname: Guo
authors.forename: Yan-kun
authors.initials: YK
authors.lastname: Shi
authors.forename: Ming-bao
authors.initials: MB
authors.lastname: Song
authors.forename: Jian-fei
authors.initials: JF
authors.lastname: Chen
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Yu
authors.forename: Yang-guang
authors.initials: YG
authors.lastname: Yin
authors.forename: Pan
authors.initials: P
authors.lastname: Gao
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: C497454:ID1 protein, rat; D051798:Inhibitor of Differentiation Protein 1
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