Development of an unsteady-state model for a biological system in miniaturized bioreactors.
In the present study, the special shake flasks, so-called ventilation flasks, are equipped with oxygen sensors and then an unsteady-state gas transfer model for shake flasks was developed and experimentally investigated for a wide range of gas transfer resistances (kplug). For the validation of our unsteady-state model to simulate the gas transfer in a biological system in the ventilation flasks, a strain of Corenobacterium glutamicum DM1730 was used as a model organism. For further easy processing, the resulting total mass-transfer resistance (kplug) is described as a function of the mass flow through the sterile plug (OTRplug) by an empirical equation. This equation is introduced into a simulation model that calculates the gas partial pressures in the headspace of the flask. Additionally, the gas transfer rates through the sterile closure and gas/liquid interface inside the flask are provided. This unsteady-state model would be a very useful method for scaling up from a shake flask to a fermentor; comparing the results of the gas concentration in the gas phase, there is good agreement between the introduced unsteady-state model and experimental results for the biological system.
Amoabediny
Ghassem
G
Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, Tehran University, PO Box 11365/4563 Tehran, Iran. amoabediny@ut.ac.ir
Ziaie-Shirkolaee
Yaser
Y
Büchs
Jochen
J
D000332:Aerobiosis / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003198:Computer Simulation; D048230:Corynebacterium glutamicum / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010100:Oxygen; D010313:Partial Pressure; D015203:Reproducibility of Results
D003470:Culture Media; D010100:Oxygen