Cloning and characterization of a SnRK1-encoding gene from Malus hupehensis Rehd. and heterologous expression in tomato.
Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) plays an important role in metabolic regulation in plant. To understand the molecular mechanism of amino acids and carbohydrate metabolism in Malus hupehensis Rehd. var. pinyiensis Jiang (Pingyi Tiancha, PYTC), a full-length cDNA clone encoding homologue of SnRK1 was isolated from PYTC by Rapid Amplification of cDNA Ends (RACE). The clone, designated as MhSnRK1, contains 2063 nucleotides with an open reading frame of 1548 nucleotides. The deduced 515 amino acids showed high identities with other plant SnRK1 genes. Quantitative real-time PCR analysis revealed this gene was expressed in roots, stems and leaves. Exposing seedlings to nitrate caused and initial decrease in expression of the MhSnRK1 gene in roots, leaves and stems in short term. Ectopic expression of MhSnRK1 in tomato mainly resulted in higher starch content in leaf and red-ripening fruit than wild-type plants. This result supports the hypothesis that overexpression of SnRK1 causes the accumulation of starch in plant cells. All the results suggest that MhSnRK1 may play important roles in carbohydrate and amino acid metabolisms.
Li
Guangjie
G
College of Science and Engineering Horticulture, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018, China.
Peng
Futian
F
Zhang
Lin
L
Shi
Xingzheng
X
Wang
Zhaoyan
Z
D003001:Cloning, Molecular; D018744:DNA, Plant; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D027845:Malus / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013213:Starch / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection
D018744:DNA, Plant; D013213:Starch; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases
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