Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus Panicovirus.
The complete nucleotide sequence of the Phleum isolate of cocksfoot mild mosaic virus (CMMV-P) and the coat protein sequence of the cocksfoot isolate (CMMV-1) were determined. Comparative sequence analysis revealed a close relationship with Panicum mosaic virus (PMV; genus Panicovirus), and together with serological studies, the work supports the classification of CMMV in the family Tombusviridae, genus Panicovirus rather than, as is currently proposed, the genus Sobemovirus. A full-length cDNA clone was prepared, and RNA transcripts synthesised from cDNA were shown to be infectious when inoculated to Hordeum vulgare.
Ziegler
Angelika
A
Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK. aziegl@scri.ac.uk
Cowan
Graham
G
Torrance
Lesley
L
D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000235:genetics; D001467:Hordeum / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D020539:Sequence Analysis, Protein; D017423:Sequence Analysis, RNA; D019183:Tombusviridae / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*
D036022:Capsid Proteins