Molecular identification of Aspergillus species collected for the Transplant-Associated Infection Surveillance Network.
A large aggregate collection of clinical isolates of aspergilli (n = 218) from transplant patients with proven or probable invasive aspergillosis was available from the Transplant-Associated Infection Surveillance Network, a 6-year prospective surveillance study. To determine the Aspergillus species distribution in this collection, isolates were subjected to comparative sequence analyses by use of the internal transcribed spacer and beta-tubulin regions. Aspergillus fumigatus was the predominant species recovered, followed by A. flavus and A. niger. Several newly described species were identified, including A. lentulus and A. calidoustus; both species had high in vitro MICs to multiple antifungal drugs. Aspergillus tubingensis, a member of the A. niger species complex, is described from clinical specimens; all A. tubingensis isolates had low in vitro MICs to antifungal drugs.
Balajee
S Arunmozhi
SA
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. fir3@cdc.gov
Kano
Rui
R
Baddley
John W
JW
Moser
Stephen A
SA
Marr
Kieren A
KA
Alexander
Barbara D
BD
Andes
David
D
Kontoyiannis
Dimitrios P
DP
Perrone
Giancarlo
G
Peterson
Stephen
S
Brandt
Mary E
ME
Pappas
Peter G
PG
Chiller
Tom
T
D000935:Antifungal Agents / Q000494:pharmacology; D001228:Aspergillosis / Q000382:microbiology*; D001230:Aspergillus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006801:Humans; D016867:Immunocompromised Host; D008826:Microbial Sensitivity Tests; D017422:Sequence Analysis, DNA; D014180:Transplantation / Q000009:adverse effects*; D014404:Tubulin / Q000235:genetics
D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D014404:Tubulin
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