Somatic embryogenesis in apomict Boechera holboellii.
In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4-6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA - 6-benzylaminopurine; NAA - alpha-naphthaleneacetic acid; TDZ - 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 muM NAA + 8.87 muM BA and 2.68 muM NAA + 17.74 muM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 muM NAA + 4.44 muM BA produced somatic embryos at a frequency of 38%.
Taskin
K M
KM
Department of Biology, Canakkale Onsekiz Mart University, 17100 Canakkale Turkey. kmtaskin@gmail.com
Turgut
K
K
Scott
R J
RJ
D019607:Brassicaceae / Q000033:anatomy & histology / Q000196:embryology* / Q000502:physiology; D018548:Cotyledon / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development; D012038:Regeneration; D012100:Reproduction, Asexual; D046509:Tissue Culture Techniques