Determination of Tn antigen released from cultured cancer cells by capillary electrophoresis.
An incomplete elongation of O-glycans in mucins has been found in epithelial cancers, leading to the expression of shorter carbohydrate structures such as Tn antigen (GalNAc-O-Ser/Thr), which has been reported to be one of the most specific human cancer-associated structures. However, there have been no appropriate physicochemical methods for the determination of Tn antigen in biological samples. In the present paper, we developed a capillary electrophoresis method for the determination of Tn antigen, and applied the method to the analysis of the expressed Tn antigen on some leukemia and epithelial cancer cells.
Yamada
Keita
K
School of Pharmacy, Kinki University, Kowakae 3-4-1, Higashi-Osaka 577-8502, Japan.
Watanabe
Sakie
S
Kita
Soichiro
S
Kinoshita
Mitsuhiro
M
Hayakawa
Takao
T
Kakehi
Kazuaki
K
D000116:Acetylgalactosamine / Q000302:isolation & purification; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D014407:Tumor Cells, Cultured; D062367:ortho-Aminobenzoates / Q000378:metabolism
D015295:Antigens, Tumor-Associated, Carbohydrate; C040286:Tn antigen; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D000116:Acetylgalactosamine