title: Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidicnanofluidic devices.
abstract: This paper presents a study of electrokinetic transport in single nanopores integrated into vertically stacked three-dimensional hybrid microfluidicnanofluidic structures. In these devices, single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e., DNA) polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electro-osmotic transport is predominant over electrophoresis in single nanopores with d>180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.
authors.forename: Travis L
authors.initials: TL
authors.lastname: King
authors.forename: Enid N
authors.initials: EN
authors.lastname: Gatimu
authors.forename: Paul W
authors.initials: PW
authors.lastname: Bohn
references.citation: Phys Rev E Stat Nonlin Soft Matter Phys. 2006 Oct;74(4 Pt 1):041901
references.pmid: 17155090
references.citation: Analyst. 2003 Jun;128(6):537-42
references.pmid: 12866863
references.citation: J Phys Chem B. 2008 Nov 27;112(47):14687-91
references.pmid: 18973375
references.citation: Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9127-32
references.pmid: 15961544
references.citation: Biophys J. 2008 Jul;95(1):273-86
references.pmid: 18339746
references.citation: Lab Chip. 2006 May;6(5):667-74
references.pmid: 16652183
references.citation: Sens Actuators B Chem. 2008 Jun 16;132(2):593-600
references.pmid: 19584949
references.citation: Langmuir. 2007 Dec 18;23(26):13209-22
references.pmid: 17999544
references.citation: Anal Chem. 2006 Apr 1;78(7):2335-41
references.pmid: 16579617
references.citation: Nano Lett. 2008 Jun;8(6):1683-8
references.pmid: 18459741
references.citation: Lab Chip. 2008 Oct;8(10):1625-31
references.pmid: 18813383
references.citation: Phys Rev Lett. 2007 Jun 8;98(23):238104
references.pmid: 17677940
references.citation: J Am Chem Soc. 2005 Oct 12;127(40):13928-33
references.pmid: 16201814
references.citation: J Am Chem Soc. 2007 Jun 20;129(24):7620-6
references.pmid: 17530757
references.citation: Angew Chem Int Ed Engl. 2004 Mar 26;43(14):1862-5
references.pmid: 15054797
references.citation: Anal Chem. 2004 Nov 1;76(21):6419-25
references.pmid: 15516136
references.citation: Rev Sci Instrum. 2008 Jun;79(6):063702
references.pmid: 18601408
references.citation: Phys Rev Lett. 2008 Apr 18;100(15):158302
references.pmid: 18518160
references.citation: J Phys Chem B. 2007 Jul 26;111(29):8477-85
references.pmid: 17604391
references.citation: Biophys J. 2006 Jun 15;90(12):4538-45
references.pmid: 16732056
references.citation: J Chem Phys. 2007 Apr 21;126(15):154904
references.pmid: 17461664