NMR characterization for polysaccharide moiety of a glycopeptide.
Ganoderma lucidum is a traditional medicinal herb used to treat various diseases in China and Southeast Asia for thousands of years. An aqueous glycopeptide, LZ-B-1, was prepared by successive chromatography and exhibited an immunostimulating potential. To better understand the mechanism of bioactivity for this compound, the polysaccharide moiety of glycopeptide LZ-B-1 was studied by NMR spectroscopy. The results indicated that the polysaccharide moiety had a backbone of 1,6-disubstituted-alpha-galactopyranosyl, 1,2,6-trisubstituted-alpha-galactopyranosyl, 1,3-disubstituted-beta-glucopyranosyl and 1,4,6-trisubstituted-beta-glucopyranosyl residues. The branches were mainly composed of 1-substituted-beta-glucopyranosyl and 1-substituted-alpha-fucopyranosyl residues.
Ye
Libin
L
Department of Biology, College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou 310035, China.
Li
Jianrong
J
Zhang
Jingsong
J
Pan
Yingjie
Y
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry*; D020940:Reishi / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D006020:Glycopeptides; C550891:LZ-B-1 glycopeptide; D011134:Polysaccharides