Co-translational mRNA decay in Saccharomyces cerevisiae.
The rates of RNA decay and transcription determine the steady-state levels of all messenger RNA and both can be subject to regulation. Although the details of transcriptional regulation are becoming increasingly understood, the mechanism(s) controlling mRNA decay remain unclear. In yeast, a major pathway of mRNA decay begins with deadenylation followed by decapping and 5'-3' exonuclease digestion. Importantly, it is hypothesized that ribosomes must be removed from mRNA before transcripts are destroyed. Contrary to this prediction, here we show that decay takes place while mRNAs are associated with actively translating ribosomes. The data indicate that dissociation of ribosomes from mRNA is not a prerequisite for decay and we suggest that the 5'-3' polarity of mRNA degradation has evolved to ensure that the last translocating ribosome can complete translation.
Hu
Wenqian
W
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Sweet
Thomas J
TJ
Chamnongpol
Sangpen
S
Baker
Kristian E
KE
Coller
Jeff
J
D011061:Poly A / Q000378:metabolism; D026723:Polyadenylation; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000378:metabolism; D020871:RNA Stability; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012315:RNA Caps; D012331:RNA, Fungal; D012333:RNA, Messenger; D011061:Poly A
Cell. 2005 Sep 23;122(6):875-86
16179257
Methods. 1999 Jan;17(1):28-37
10075880
Mol Cell. 2007 Mar 9;25(5):635-46
17349952
Annu Rev Biochem. 2004;73:861-90
15189161
J Biol Chem. 1994 Apr 1;269(13):9687-92
8144558
Mol Cell. 2008 Dec 5;32(5):605-15
19061636
Nature. 2008 Jun 26;453(7199):1276-80
18496529
Mol Cell Biol. 2007 Jun;27(11):3970-81
17403906
Mol Cell Biol. 1995 Apr;15(4):2145-56
7891709
Methods Enzymol. 2001;342:251-9
11586897
PLoS Biol. 2006 Jul;4(7):e210
16756390
Nature. 2006 Mar 23;440(7083):561-4
16554824
J Cell Biol. 2007 Nov 5;179(3):437-49
17984320
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5628-33
9159123
Annu Rev Biochem. 1996;65:693-739
8811193
Methods Enzymol. 2000;317:109-23
10829275
Mol Cell Biol. 1993 Aug;13(8):4826-35
8336719
Mol Cell Biol. 2001 Mar;21(5):1515-30
11238889
RNA. 1997 Mar;3(3):234-44
9056761
RNA. 2002 Jul;8(7):948-58
12166649
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12663-8
12218187
Nat Rev Mol Cell Biol. 2007 Jan;8(1):9-22
17183357
RNA. 2007 Apr;13(4):493-502
17307817