The localization of the Golgin GCC185 is independent of Rab6A/A' and Arl1.
Mammalian golgins of the trans-Golgi network (TGN) are small G protein effectors that are required for membrane transport and contain a Golgi targeting C-terminal GRIP domain. The localization of two TGN golgins, p230/golgin-245 and golgin-97, is mediated by the small GTPase Arl1, whereas recruitment of the TGN golgin GCC185 is controversial. Recently, GCC185 was proposed to localize to the Golgi by the co-operation of two small GTPases, Rab6A/A' and Arl1 (Burguete et al., 2008), a model based predominantly on in vitro interactions. Here we demonstrate that Golgi recruitment of endogenous GCC185 does not involve Rab6A/A' and Arl1. We find minimal colocalization between Rab6A/A' and endogenous GCC185 on Golgi membranes and failed to detect an interaction between Rab6A/A' and C-terminal domains of GCC185 by yeast two-hybrid analyses. Moreover, depletion of both Rab6A/A' and Arl1 also had no effect on the localization of endogenous GCC185 or the isolated GRIP domain of GCC185.
Houghton
Fiona J
FJ
The Department of Biochemistry and Molecular Biology, The University of Melbourne, Melbourne, Victoria 3010, Australia.
Chew
Pau Ling
PL
Lodeho
Sylvain
S
Goud
Bruno
B
Gleeson
Paul A
PA
D020727:ADP-Ribosylation Factors / Q000032:analysis / Q000378:metabolism*; D003600:Cytosol; D006056:Golgi Apparatus / Q000737:chemistry*; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D021381:Protein Transport; D020691:rab GTP-Binding Proteins / Q000032:analysis / Q000378:metabolism*
C472299:GCC2 protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; C094135:Rab6 protein; C097313:ADP-ribosylation factor related proteins; D020727:ADP-Ribosylation Factors; D020691:rab GTP-Binding Proteins