Early suppression of NFkappaB and IL-8 in bronchial epithelium after ozone exposure in healthy human subjects.
Exposure to elevated concentrations of ozone, a common air pollutant, has been associated with numerous adverse health effects. We have previously reported the time-course of ozone-induced airway inflammation, demonstrating an early up-regulation of vascular endothelial adhesion molecules in bronchial mucosa at 1.5 hours, followed by a neutrophilic infiltration 6 hours after exposure to 0.2 ppm ozone. We hypothesized that the neutrophilic infiltration in the bronchial mucosa would reflect an early increase in bronchial epithelial expression of redox-sensitive transcription factors and kinases regulating neutrophil chemoattractant expression. To test this hypothesis, endobronchial biopsies were obtained from healthy human subjects (n = 11) 1.5 hours after 0.2 ppm of ozone and filtered air exposures (lasting for 2 hours) and stained for mitogen-activated protein kinases (MAPKs), transcription factors, and neutrophil chemoattractants. Total epithelial staining was quantified, as well as the extent of nuclear translocation. Contrary to expectation, ozone significantly suppressed total and nuclear expression of nuclear factor kappaB (NFkappaB) in bronchial epithelial cells (p = 0.02 and p = 0.003 respectively). Similarly, the total staining for phosphorylated C-jun was suppressed (p = 0.021). Expression of interleukin 8 (IL-8) in the bronchial epithelium was likewise decreased after ozone (p = 0.018), while GRO-alpha, ENA-78, C-fos, p-p38, p-JNK, and p-ERK stainings were unchanged. These data suggest that the redox-sensitive NFkappaB and activator protein 1 (AP-1) pathways within the human bronchial epithelium do not seem to be involved in the early inflammatory cell recruitment pathways in healthy subjects exposed to ozone.
Bosson
Jenny
J
Department of Respiratory Medicine and Allergy, University Hospital, Umeå, Sweden. jenny.bosson@gmail.com
Blomberg
Anders
A
Pourazar
Jamshid
J
Mudway
Ian S
IS
Frew
Anthony J
AJ
Kelly
Frank J
FJ
Sandström
Thomas
T
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D001999:Bronchoscopy; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019570:Inhalation Exposure; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D020556:Neutrophil Infiltration / Q000187:drug effects; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000037:antagonists & inhibitors / Q000633:toxicity*; D020545:Respiratory Mucosa / Q000187:drug effects / Q000378:metabolism*; D055815:Young Adult
D000975:Antioxidants; D016207:Cytokines; D016209:Interleukin-8; D016328:NF-kappa B; D010083:Oxidants, Photochemical; D010126:Ozone; D020928:Mitogen-Activated Protein Kinases