Distribution of Helicobacter pylori cagA, cagE, oipA and vacA in different major ethnic groups in Tehran, Iran.
OBJECTIVE
There are geographical variations in Helicobacter pylori virulence genes; cagA, cagE, vacA and oipA. The present study compared the distribution of these genotypes in major ethnic groups residing in Tehran, Iran and their association with clinical outcomes.


METHODS
A total of 124 H. pylori-positive patients living in Tehran were enrolled in this study. The ethnic distribution was 74 Persians, 33 Turks and 17 other ethnics including Kurds, Lurs, Afghanis and Arabs. The presence of the cagA, cagE and oipA genes and vacA alleles (signal [s] and middle [m] region) were determined by polymerase chain reaction (PCR) from H. pylori DNA.


RESULTS
The cagA-positive status was predominant in all three ethnic groups (e.g. 65% in Persians and 73% in Turks). In contrast, the cagE-positive status was less than half in Persians (47%) and Turks (30%), whereas it was 77% in other ethnicities (P = 0.008). The predominant vacA genotypes were s1 and m1 in all three ethnic groups (e.g. 68% in Persians and 70% in Turks were s1). There was no significant association between cagA and cagE status or vacA genotypes and clinical outcomes. The oipA-positive strains were more common in non-ulcer dyspepsia (NUD) (63%) than in peptic ulcer patients (15%) (P = 0.001) in Persians, but the association was not observed in other ethnic groups.


CONCLUSIONS
There are some differences in the H. pylori genotypes among the ethnic groups in Iran. However, none of these markers seemed to be clinically helpful in predicting the clinical presentation of a H. pylori infection in Iran.
Dabiri
Hossein
H
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Maleknejad
Parviz
P
Yamaoka
Yoshio
Y
Feizabadi
Mohammad M
MM
Jafari
Fereshteh
F
Rezadehbashi
Maryam
M
Nakhjavani
Farrokh A
FA
Mirsalehian
Akbar
A
Zali
Mohammad R
MR
D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004415:Dyspepsia / Q000208:ethnology / Q000382:microbiology; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005773:Gastroscopy; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications / Q000208:ethnology* / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010437:Peptic Ulcer / Q000208:ethnology / Q000382:microbiology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018570:Risk Assessment; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000208:ethnology / Q000382:microbiology; D014505:Urban Population; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C526444:OipA protein, Helicobacter pylori; C103937:PicB protein, Helicobacter pylori; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori
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