Trypanosoma cruzi: biodistribution of technetium-99m pertechnetate in infected rats.
With the aim of investigating the biodistribution of technetium-99m pertechnetate ((99m)TcO4-) in rats infected with Y strain of Tripanosoma Cruzi, at the peak of parasitemia, (14th day of infection), we injected Wistar rats with 0.1 ml of (99m)TcO4- (3.7MBq). After 60 min, the percentage of radioactivity per gram was counted in several isolated organs and blood, using a gamma counter (1470 Wizard, PerkinElmer Finland). The uptake of (99m)TcO4- increased significantly in blood and decreased in the colon of infected animals (p<0.05). A significant reduction in serum iron and red blood cells and a significant increase in total proteins, leukocytes and lymphocytes in the infected rats were observed, compared with controls (p<0.05). A reduction in muscle layer thickness of the colon and mononuclear inflammation were observed. These results conclusively demonstrate that T. cruzi infection would be associated with changes in the biodistribution of (99m)TcO4- and in colon morphology, with potential clinical implications.
Barbosa
Vanessa S A
VS
Postgraduate Program for Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil.
Holanda
Cecília M C X
CM
Câmara
Antônia C J
AC
Silva
Roseane P
RP
Oliveira
Daniel P
DP
Moreira
Jeanete A
JA
Medeiros
Aldo C
AC
D000818:Animals; D014355:Chagas Disease / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000469:parasitology; D008297:Male; D018512:Parasitemia / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000469:parasitology; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014349:Trypanosoma cruzi / Q000502:physiology*
D019275:Radiopharmaceuticals; D013670:Sodium Pertechnetate Tc 99m