title: Changes in biomass activity and characteristics of activated sludge exposed to low ozone dose.
abstract: In this paper, the response mechanism of activated sludge exposed to low-dose ozone at less than 20mgO(3)g(-1) total suspended solids (TSS) was studied by analyzing the changes in sludge activity and the evolution of C, N, P and metals from sludge following ozonation. The intracellular ATP concentration was not affected at less than 5mgO(3)g(-1) TSS and thereafter decreased rapidly to around 60% when the ozone dose increased to 20mgO(3)g(-1) TSS. Similarly, the efficiency of sludge solubilization initially changed a little and then increased rapidly to around 30% at an ozone dose of 20mgO(3)g(-1) TSS. However, the activities of superoxide dismutase and protease decreased immediately upon exposure to ozone. These findings indicate that ozone firstly destroys the floc, leading to the disruption of the compact aggregates, which does not affect cells viability but induces a decrease in enzyme activities. Ozone then attacks the bacterial cells of the sludge, causing a decrease in cells viability. During ozonation, the content of carbon, nitrogen and phosphorus in the sludge matrix decreased, while the content of these elements in the micro-solids and supernatant gradually increased. Most of the released metals from the sludge matrix were found in the micro-solids.
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, China. chulibing@tsinghua.edu.cn
authors.forename: Libing
authors.initials: L
authors.lastname: Chu
authors.forename: Jianlong
authors.initials: J
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Xing
authors.forename: Sangtian
authors.initials: S
authors.lastname: Yan
authors.forename: Xulin
authors.initials: X
authors.lastname: Sun
authors.forename: Benjamin
authors.initials: B
authors.lastname: Jurcik
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D019216:Metals, Heavy / Q000378:metabolism; D010126:Ozone / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D019216:Metals, Heavy; D012722:Sewage; D010126:Ozone; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D010447:Peptide Hydrolases