title: Bioleaching of metals from printed wire boards by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans and their mixture.
abstract: Bioleaching processes were used to mobilize metals from printed wire boards (PWBs). The bacteria Acidithiobacillus ferrooxidans (A. ferrooxidans) and Acidithiobacillus thiooxidans (A. thiooxidans) isolated from an acidic mine drainage were grown and acclimated in presence of PWBs and then used as bioleaching bacteria to solubilize metals from PWBs. The experimental results demonstrate that all the percentages of copper, lead, zinc solubilized into the leaching solution from actual PWBs basically increased with decrease of sieve fraction of sample and decrease of PWBs concentration. The concentration of PWBs should be controlled under the range from 7.8 to 19.5 g l(-1). Under 7.8 g l(-1) of the concentration of PWBs, the percentages of copper solubilized are 99.0%, 74.9%, 99.9% at 0.5-1.0mm of sieve fraction at 9 d of leaching time by the pure culture of A. ferrooxidans, the pure culture of A. thiooxidans, and mixed culture of A. ferrooxidans and A. thiooxidans, respectively, while the percentages of copper, lead and zinc solubilized are all more than 88.9% at <0.35 mm of the sieve fractions of sample at 5d of leaching time by the above three kinds of cultures. Variation of pH and redox potential of leaching solution with time implied that Fe(3+) oxidized from Fe(2+) in the culture medium in presence of A. ferrooxidans caused the mobilization of metals. It is concluded that A. ferrooxidans and A. thiooxidans were able to grow in the presence of PWBs and the pure culture of A. ferrooxidans, and the mixed culture of A. ferrooxidans and A. thiooxidans can not only efficiently bioleach the main metal copper but also bioleach other minor metals such as lead, zinc as well.
authors.affiliation: School of Urban Development and Environmental Engineering, Shanghai Second Polytechnic University, 201209 Shanghai, People's Republic of China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Wang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Bai
authors.forename: Jinqiu
authors.initials: J
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Liang
mesh_terms: D042763:Acidithiobacillus / Q000378:metabolism*; D013856:Acidithiobacillus thiooxidans / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003300:Copper / Q000378:metabolism; D055615:Electrical Equipment and Supplies / Q000382:microbiology*; D007220:Industrial Waste; D007854:Lead / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D012995:Solubility; D015032:Zinc / Q000378:metabolism
chemical_list: D007220:Industrial Waste; D008670:Metals; D007854:Lead; D003300:Copper; D015032:Zinc