Rotational order-disorder structure of fluorescent protein FP480.
In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order-disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90 degrees with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.
Pletnev
Sergei
S
SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, Argonne, IL 60439, USA. svp@ncifcrf.gov
Morozova
Kateryna S
KS
Verkhusha
Vladislav V
VV
Dauter
Zbigniew
Z
D019295:Computational Biology; D003460:Crystallization; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism; D009905:Optical Rotatory Dispersion; D013237:Stereoisomerism
D008164:Luminescent Proteins; D046911:Macromolecular Substances
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):83-95
16369097
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
15572765
Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):67-74
15608377
Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):926-30
17642519
Acta Crystallogr D Biol Crystallogr. 2008 Aug;D64(Pt 8):843-50
18645233
Acta Crystallogr D Biol Crystallogr. 2008 Jul;D64(Pt 7):792-802
18566514
Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1124-30
12832754
Acta Crystallogr A. 2008 Jan;64(Pt 1):112-22
18156677
Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41
15983416
Methods Enzymol. 1997;276:344-58
9048378
J Mol Biol. 1993 Dec 20;234(4):1098-118
8263915
Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):687-92
15299632
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
15299926
Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1079-90
11468391
Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21
9757107
Nucleic Acids Res. 2000 Jan 1;28(1):235-42
10592235
Methods Enzymol. 1997;276:307-26
27754618
Science. 2008 Feb 22;319(5866):1083-6
18292340
Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):967-75
15983420
Acta Crystallogr D Biol Crystallogr. 2002 Nov;58(Pt 11):1948-54
12393927
J Biol Chem. 2006 Nov 10;281(45):34610-6
16954221