An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis.
In determining the efficacy of new vaccine candidates for leptospirosis, the primary end point is death and an important secondary end point is sterilizing immunity. However, evaluation of this end point is often hampered by the time-consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with Leptospira interrogans serovar Copenhageni. In a dissemination study, compared to CI, the IM led to equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and overall concordance was good (kappa=0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant leptospiral protein-based vaccine candidate and subsequently challenged, the agreement between the CI and IM was very good (kappa=0.84). These findings indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates.
Chagas-Junior
Adenizar D
AD
Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil.
McBride
Alan J A
AJA
Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil.
Athanazio
Daniel A
DA
Department of Biointeraction, Health Sciences Institute, Federal University of Bahia, Salvador, BA, Brazil.|Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil.
Figueira
Cláudio P
CP
Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil.
Medeiros
Marco A
MA
Bio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Rio de Janeiro, RJ, Brazil.
Reis
Mitermayer G
MG
Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil.
Ko
Albert I
AI
Division of Infectious Diseases, Weill Medical College of Cornell University, NY, USA.|Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil.
McBride
Flávia W C
FWC
Department of Biointeraction, Health Sciences Institute, Federal University of Bahia, Salvador, BA, Brazil.|Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil.
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D006224:Cricetinae; D005260:Female; D007668:Kidney / Q000382:microbiology; D007921:Leptospira interrogans / Q000145:classification / Q000302:isolation & purification*; D007922:Leptospirosis / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
D001428:Bacterial Vaccines
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