Induction of complement-mediated lysis of HIV-1 by a combination of HIV-specific and HLA allotype-specific antibodies.
HLA-specific antibodies generated by allo-immunization are supposed to be involved in the control of HIV infections by both the neutralizing capacity of HLA-specific antibodies (Abs) and HLA-specific Ab-dependent complement-mediated lysis (CML). We further characterized CML of HIV primary isolates induced by HLA-specific Abs. Although HIV-specific and HLA allo-type specific Abs induced only weak CML of HIV primary isolates, several combinations of HLA allo-type specific Abs with HIV-specific Abs could enhance CML significantly. Nevertheless, certain HLA-specific Abs did not improve but even inhibit CML of HIV, although the corresponding HLA molecules were present. Thus, our results emphasize a possible limitation of allo-immunization as a potential approach to induce protective immunity against HIV.
Hildgartner
Alexander
A
Wilflingseder
Doris
D
Gassner
Christoph
C
Dierich
Manfred P
MP
Stoiber
Heribert
H
Bánki
Zoltán
Z
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000628:therapy / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007167:Immunotherapy / Q000379:methods; D051379:Mice
D015483:HIV Antibodies; D006680:HLA Antigens; D007126:Immunoglobulin Allotypes; D003165:Complement System Proteins