Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae.
Arabidopsis thaliana was used as an experimental model plant to investigate a tritrophic interaction between the plant, a specialist aphid herbivore, Brevicoryne brassicae, and its natural enemy, the parasitoid Diaeretiella rapae. The A. thaliana ecotype Col-5 was transformed with a functional 2-oxoglutarate dependent dioxygenase (BniGSL-ALK) that converts 3-methylsulfinylpropylglucosinolate and 4-methylsulfinylbutylglucosinolate to 2-propenylglucosinolate and 3-butenylglucosinolate, respectively. This transformation results in a change in the glucosinolate hydrolysis profile where 3-butenylisothiocyanate, 2-propenylisothiocyanate and 5-vinyloxazolidine-2-thione are produced in contrast to the wild-type plant where 4-methylsulfinylbutylisothiocyanate is the main product. Performance of B. brassicae was affected negatively by transforming Col-5 with BniGSL-ALK in terms of mean relative growth rates. In a series of behavioral bioassays, naïve D. rapae females were able to discriminate between B. brassicae infested and uninfested Col-5 plants transformed with BniGSL-ALK, with parasitoids showing a preference for B. brassicae infested plants. By contrast, naïve D. rapae females were unable to discriminate between aphid infested and uninfested Col-5 plants. Subsequent air entrainments of B. brassicae infested Col-5 plants transformed with BniGSL-ALK further confirmed the presence of 3-butenylisothiocyanate in the headspace. By contrast, no glucosinolate hydrolysis products were recorded from similarly infested Col-5 plants.
Kissen
Ralph
R
Division of Biology, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, UK.
Pope
Tom W
TW
Grant
Murray
M
Pickett
John A
JA
Rossiter
John T
JT
Powell
Glen
G
D000818:Animals; D001042:Aphids / Q000254:growth & development*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000469:parasitology; D029681:Arabidopsis Proteins / Q000378:metabolism; D005260:Female; D005961:Glucosinolates / Q000737:chemistry*; D006790:Host-Parasite Interactions; D006927:Hymenoptera / Q000254:growth & development*; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified
D029681:Arabidopsis Proteins; D005961:Glucosinolates
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