Interactions of single-wall carbon nanotubes with endothelial cells.
Single-wall carbon nanotubes (SWCNTs) could be promising delivery vehicles for cancer therapy. These carriers are generally introduced intravenously, however, little is known of their interactions with endothelial cells, the cells lining vessels and mediating clearance of nanoparticles. Here we show that SWCNTs of 1 to 5 microm in length, both "pristine" and functionalized by oxidation, had limited toxicity for endothelial cells in vitro as determined by growth, migration morphogenesis, and survival assays. Endothelial cells transiently took up SWCNTs, and several lines of data indicated that they were associated with an enhanced acidic vesicle compartment within the endothelial cells. Our findings of SWCNT interactions with endothelial cells suggest these may be optimal vehicles for targeting the vasculature and potential carriers of anti-angiogenic drugs. The implications on their biological activity must be taken into account when considering the use of these nanoparticles for therapeutic delivery of drugs.


BACKGROUND
Interactions of single walled carbon nanotubes (SWCNTs) with endothelial cells following IV administration remains unclear. Functionalized and naïve SWCNTs of 1-5 mm in length had limited toxicity to endothelial cells in vitro. Endothelial cells transiently took up SWCNTs and were associated with an enhanced acidic vesicle compartment within the cells. These findings suggest that SWCNTs may be promising vehicles for targeting the vasculature and potential carriers of anti-angiogenic drugs.
Albini
Adriana
A
Polo Scientifico e Tecnologico, IRCCS Multimedica, Milano, Italy.
Mussi
Valentina
V
Parodi
Alessandro
A
Ventura
Agostina
A
Principi
Elisa
E
Tegami
Sara
S
Rocchia
Massimiliano
M
Francheschi
Enrico
E
Sogno
Ilaria
I
Cammarota
Rosaria
R
Finzi
Giovanna
G
Sessa
Fausto
F
Noonan
Douglas McClain
DM
Valbusa
Ugo
U
D002478:Cells, Cultured; D004705:Endocytosis / Q000502:physiology*; D042783:Endothelial Cells / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity
D037742:Nanotubes, Carbon