title: Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells.
abstract: We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital, China Medical University, Shenyang , China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Huang
authors.forename: M J
authors.initials: MJ
authors.lastname: Sun
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: B Y
authors.initials: BY
authors.lastname: Fu
mesh_terms: D000818:Animals; D005934:Glucagon / Q000378:metabolism*; D050416:Glucagon-Secreting Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D010191:Pancreatic Polypeptide / Q000378:metabolism*; D050418:Pancreatic Polypeptide-Secreting Cells / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010191:Pancreatic Polypeptide; D005934:Glucagon
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