In vitro and in vivo anti-photoaging effects of an isoflavone extract from soybean cake.
BACKGROUND
Soy has been used in traditional Chinese medicine for thousands of years for its health and nutritional benefits, as well as to treat and care for the skin. Advanced skin care research has shown that soy isoflavone and genistein are effective in reducing damage to the skin from the sun.


OBJECTIVE
To study the protective effects of isoflavone extract from soybean cake against the UVB-induced skin damage.


METHODS
The in vitro effects and possible mechanisms of soybean extract on UVB protection were determined in HaCaT cells. In the in vivo study, ICR-Foxn/(nu) mice were irradiated with UVB. The epidermal thickness, catalase and the expressions of cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) were detected to evaluate the antioxidant and anti-inflammatory activities of the isoflavone extract.


RESULTS
Our in vitro studies showed that UVB-induced HaCaT cell death and the phosphorylation of p38, JNK, and ERK1/2 decreased in the presence of isoflavone extract. In the in vivo studies, we found that the topical application of isoflavone extract before UVB irritation decreased the epidermal thickness and the expressions of COX-2 and PCNA and increased catalase concentration. These results showed anti-photoaging effect of isoflavone extract from soybean cake involved the inhibition of UVB-induced apoptosis and inflammation.


CONCLUSIONS
It is shown that isoflavone extract from soybean cake may be functional cosmeceutical candidate for skin photoaging.
Chiu
Tsu-Man
TM
Department of Dermatology, Changhua Christian Hospital, Changhua Hsien, Taiwan, ROC.
Huang
Chieh-Chen
CC
Lin
Tzu-Ju
TJ
Fang
Jia-You
JY
Wu
Nan-Lin
NL
Hung
Chi-Feng
CF
D000818:Animals; D002374:Catalase / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004890:Erythema; D019833:Genistein / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012867:Skin / Q000033:anatomy & histology / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D013025:Glycine max / Q000737:chemistry*; D014466:Ultraviolet Rays
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D018809:Proliferating Cell Nuclear Antigen; D019833:Genistein; D002374:Catalase; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2