Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck.
Cytokinesis in yeast can be achieved by plasma membrane ingression, which is dependent on actomyosin ring constriction. Inn1 presumably couples these processes by interaction with both the plasma membrane and the temporary actomyosin ring component Hof1. In addition, an actomyosin ring independent cytokinesis pathway exists in yeast. We here identified Cyk3, a key component of the alternative pathway, as a novel interaction partner of Inn1. The carboxy-terminal proline rich part of Inn1 binds the SH3 domains of either Cyk3 or Hof1. Strains with truncated proteins lacking either of these SH3 domains do not display any severe phenotypes, but are synthetically lethal, demonstrating their crucial role in cytokinesis. Overexpression of CYK3 leads to an actomyosin ring independent recruitment of Inn1 to the bud neck, further supporting the significance of this interaction in vivo. Moreover, overexpression of CYK3 in a myo1 or an iqg1 deletion not only restores viability, but also the recruitment of Inn1 to the bud neck. We propose that Cyk3 is part of an actomyosin ring independent cytokinesis pathway, which acts as a rescue mechanism to recruit Inn1 to the bud neck.
Jendretzki
Arne
A
Universität Osnabrück, Fachbereich Biologie/Chemie, AG Genetik, Barbarastr. 11, Osnabrück 49076, Germany.
Ciklic
Ivan
I
Rodicio
Rosaura
R
Schmitz
Hans-Peter
HP
Heinisch
Jürgen J
JJ
D000205:Actomyosin / Q000378:metabolism / Q000502:physiology*; D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism*; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D030781:Organisms, Genetically Modified; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000502:physiology; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014162:Transfection
D018797:Cell Cycle Proteins; C418803:Cyk3 protein, S cerevisiae; C116847:HOF1 protein, S cerevisiae; C528682:Inn1 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D029701:Saccharomyces cerevisiae Proteins; D000205:Actomyosin
J Cell Biol. 1998 Dec 28;143(7):1947-60
9864366
Curr Opin Microbiol. 2001 Dec;4(6):681-9
11731320
Yeast. 1998 Jul;14(10):953-61
9717241
J Mol Biol. 2000 Jul 21;300(4):743-58
10891267
Cell Struct Funct. 2001 Dec;26(6):529-37
11942606
Yeast. 1986 Sep;2(3):163-7
3333305
EMBO J. 2005 Apr 6;24(7):1440-52
15775961
Genetics. 1996 Dec;144(4):1425-36
8978031
Nature. 2002 Jul 25;418(6896):387-91
12140549
Traffic. 2005 Jul;6(7):575-93
15941409
Nat Cell Biol. 2008 Apr;10(4):395-406
18344988
Mol Biol Cell. 2007 Dec;18(12):5139-53
17942599
Yeast. 2004 Jun;21(8):661-70
15197731
Nucleic Acids Res. 2002 Mar 15;30(6):e23
11884642
Mol Biol Cell. 2000 Feb;11(2):593-611
10679017
Mol Biol Cell. 2002 Aug;13(8):2747-59
12181343
Microsc Res Tech. 2000 Apr 15;49(2):168-72
10816256
J Cell Biol. 2009 Jun 15;185(6):995-1012
19528296
Sci STKE. 2004 Mar 02;2004(223):PL8
15010550
J Cell Sci. 2002 Jan 15;115(Pt 2):293-302
11839781
Microbiol Mol Biol Rev. 2006 Sep;70(3):605-45
16959963
J Cell Biol. 1998 Jan 26;140(2):355-66
9442111
Nature. 2002 Jan 10;415(6868):180-3
11805837
Curr Biol. 2000 Jul 27-Aug 10;10(15):947-50
10959846
Curr Opin Cell Biol. 1996 Feb;8(1):106-19
8791410
Science. 2002 Jan 11;295(5553):321-4
11743162
J Cell Biol. 1998 Sep 7;142(5):1301-12
9732290
J Biol Chem. 1998 Oct 23;273(43):28341-5
9774458
Curr Biol. 1997 Dec 1;7(12):921-9
9382845
Curr Genet. 1993 Nov;24(5):373-6
8299150
Eur J Cell Biol. 2006 Nov;85(11):1201-15
17005296
Nat Biotechnol. 2004 Dec;22(12):1567-72
15558047
Curr Biol. 2004 Sep 21;14(18):R806-18
15380095
Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4569-74
11283351