Improved diagnostic segregation of mantle cell lymphoma by determination of cyclin D1/D3 expression ratio in formalin-fixed tissue.
Mantle cell lymphomas (MCLs) are associated with a characteristic t(11;14)(q13;q32) chromosomal translocation. This causes the CCND1 gene on chromosome 11 to be co-localized with the immunoglobulin heavy chain gene on chromosome 14, resulting in increased expression of cyclin D1. The cyclin D1/D3 expression ratio, as an approach to segregate MCLs from other small B-cell lymphomas, has not previously been evaluated in formalin-fixed, paraffin-embedded tissue. We found that mean cyclin D3 expression was lower in MCLs (P < 0.05) than in chronic lymphocytic leukemias (CLLs), follicular lymphomas (FLs), marginal zone/mucosa-associated lymphoid tissue lymphomas (MALTs), multiple myelomas (MMs), and reactive lymph nodes. As expected, mean cyclin D1 expression was increased in MCL (P < 0.05), but in several cases the expression of cyclin D1 did overlap with the level observed in CLLs, FLs, MALTs, MMs, and reactive lymph nodes. The cyclin D1/D3 expression ratio, however, did fully separate MCLs from FLs, CLLs, and reactive lymph nodes. The mean expression ratio was also significantly different between MCL and MALT (P < 0.05), but 3 MCL cases had values overlapping those of some MALTs. The expression ratio was not significantly different between MCL and MM. In conclusion, the cyclin D1/D3 expression ratio gave an improved segregation of MCLs from CLLs, FLs, MALTs, and reactive lymph nodes, as compared with determination of cyclin D1 alone in formalin-fixed, paraffin-embedded tissue.
Mangseth
Kjersti
K
Department of Pathology, Haukeland University, Bergen, Norway.
Helgeland
Lars
L
Klos
Jan
J
Molven
Anders
A
Vintermyr
Olav Karsten
OK
D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics; D056743:Cyclin D3; D016213:Cyclins / Q000096:biosynthesis* / Q000235:genetics; D005404:Fixatives / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D020869:Gene Expression Profiling; D006801:Humans; D008221:Lymphoid Tissue / Q000473:pathology; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis*; D016612:Paraffin Embedding; D010336:Pathology / Q000379:methods*
C534696:CCND3 protein, human; D056743:Cyclin D3; D016213:Cyclins; D005404:Fixatives; D019938:Cyclin D1; D005557:Formaldehyde