ErythRED, a hESC line enabling identification of erythroid cells.
A human embryonic stem cell (hESC) line that enabled globin-expressing cells to be easily recognized would facilitate optimization of erythroid differentiation in vitro and aid in the identification of hESC-derived erythroid cells in transplanted animals. We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human beta-globin locus control region, is restricted to maturing erythroid cells.
Hatzistavrou
Tanya
T
Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.
Micallef
Suzanne J
SJ
Ng
Elizabeth S
ES
Vadolas
Jim
J
Stanley
Edouard G
EG
Elefanty
Andrew G
AG
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D055544:beta-Globins / Q000235:genetics / Q000378:metabolism; D000070584:Tripartite Motif Proteins
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D055544:beta-Globins; C064419:TRIM27 protein, human; D000070584:Tripartite Motif Proteins
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