title: Loss of calmodulin binding to Bax inhibitor-1 affects Pseudomonas-mediated hypersensitive response-associated cell death in Arabidopsis thaliana.
abstract: Bax inhibitor-1 (BI-1) is a cell death suppressor protein conserved across a variety of organisms. The Arabidopsis atbi1-1 plant is a mutant in which the C-terminal 6 amino acids of the expressed BI-1 protein have been replaced by T-DNA insertion. This mutant BI-1 protein (AtBI-CM) produced in Escherichia coli can no longer bind to calmodulin. A promoter-reporter assay demonstrated compartmentalized expression of BI-1 during hypersensitive response, introduced by the inoculation of Pseudomonas syringae possessing the avrRTP2 gene, Pst(avrRPT2). In addition, both BI-1 knockdown plants and atbi1-1 showed increased sensitivity to Pst(avrRPT2)-induced cell death. The results indicated that the loss of calmodulin binding reduces the cell death suppressor activity of BI-1 in planta.
authors.affiliation: Department of Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570; Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; Institute for Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570. Electronic address: mkawai@mail.saitama-u.ac.jp.
authors.forename: Maki
authors.initials: M
authors.lastname: Kawai-Yamada
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
authors.forename: Zenta
authors.initials: Z
authors.lastname: Hori
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
authors.forename: Taro
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Ihara-Ohori
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Tamura
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032.
authors.forename: Minoru
authors.initials: M
authors.lastname: Nagano
authors.affiliation: Department of Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570; Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032; Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Uchimiya
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000033:anatomy & histology / Q000382:microbiology* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011485:Protein Binding; D044224:Pseudomonas syringae / Q000502:physiology*
chemical_list: C491862:ATBI-1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D002147:Calmodulin; D008565:Membrane Proteins
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