Investigation of associations of Yarrowia lipolytica, Staphylococcus xylosus, and Lactococcus lactis in culture as a first step in microbial interaction analysis.
The interactions that may occur between microorganisms in different ecosystems have not been adequately studied yet. We investigated yeast-bacterium interactions in a synthetic medium using different culture associations involving the yeast Yarrowia lipolytica 1E07 and two bacteria, Staphylococcus xylosus C2a and Lactococcus lactis LD61. The growth and biochemical characteristics of each microorganism in the different culture associations were studied. The expression of genes related to glucose, lactate, and amino acid catabolism was analyzed by reverse transcription followed by quantitative PCR. Our results show that the growth of Y. lipolytica 1E07 is dramatically reduced by the presence of S. xylosus C2a. As a result of a low amino acid concentration in the medium, the expression of Y. lipolytica genes involved in amino acid catabolism was downregulated in the presence of S. xylosus C2a, even when L. lactis was present in the culture. Furthermore, the production of lactate by both bacteria had an impact on the lactate dehydrogenase gene expression of the yeast, which increased up to 30-fold in the three-species culture compared to the Y. lipolytica 1E07 pure culture. S. xylosus C2a growth dramatically decreased in the presence of Y. lipolytica 1E07. The growth of lactic acid bacteria was not affected by the presence of S. xylosus C2a or Y. lipolytica 1E07, although the study of gene expression showed significant variations.
Mansour
S
S
Agro Paris Tech-INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires, 78850 Thiverval Grignon, France.
Bailly
J
J
Landaud
S
S
Monnet
C
C
Sarthou
A S
AS
Cocaign-Bousquet
M
M
Leroy
S
S
Irlinger
F
F
Bonnarme
P
P
D000596:Amino Acids / Q000378:metabolism; D001483:Base Sequence; D003470:Culture Media; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D017753:Ecosystem; D015870:Gene Expression; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008828:Microbiological Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013210:Staphylococcus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D025062:Yarrowia / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
D000596:Amino Acids; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial; D004271:DNA, Fungal; D019344:Lactic Acid; D005947:Glucose
Appl Environ Microbiol. 2008 Jan;74(2):485-94
17993564
Appl Environ Microbiol. 2008 Jun;74(11):3356-67
18390675
J Appl Microbiol. 2006 Sep;101(3):668-81
16907817
Microbiology (Reading). 2005 Nov;151(Pt 11):3627-3637
16272384
Microbiol Rev. 1996 Jun;60(2):280-300
8801434
Int J Food Microbiol. 1997 Apr 29;36(1):77-82
9168317
Int J Food Microbiol. 2001 Sep 19;69(1-2):25-36
11589557
J Dairy Res. 2004 Aug;71(3):346-54
15354582
Appl Environ Microbiol. 2008 Nov;74(21):6505-12
18776032
Nucleic Acids Res. 2001 May 1;29(9):e45
11328886
Plasmid. 1983 Mar;9(2):126-37
6602348
J Bacteriol. 1998 Aug;180(15):3804-8
9683475
J Mol Endocrinol. 2000 Oct;25(2):169-93
11013345
Int J Food Microbiol. 2006 Mar 1;107(1):1-11
16271787
Int J Food Microbiol. 2002 May 5;75(1-2):99-109
11999121
Appl Environ Microbiol. 2005 Nov;71(11):6489-500
16269673
Appl Environ Microbiol. 2006 Jul;72(7):4931-41
16820490
Curr Opin Microbiol. 2003 Jun;6(3):288-94
12831906
Meat Sci. 2000 Oct;56(2):105-15
22061897
Appl Environ Microbiol. 1993 Jun;59(6):1893-7
16348966
Mol Cell Biol. 1991 Jul;11(7):3762-72
2046677
Appl Environ Microbiol. 2007 Apr;73(8):2561-70
17308183
Vet Microbiol. 1995 Jul;45(2-3):275-9
7571379
Meat Sci. 2007 Dec;77(4):570-9
22061943
Appl Environ Microbiol. 2005 Dec;71(12):8016-23
16332781
Appl Environ Microbiol. 2008 Jan;74(1):172-81
17981942
Mol Cell Biol. 1995 Mar;15(3):1564-72
7862149
J Microbiol Methods. 2007 Aug;70(2):355-62
17590462
Appl Environ Microbiol. 2003 Aug;69(8):4777-87
12902271
Meat Sci. 2003 Oct;65(2):859-67
22063449
J Dairy Sci. 2005 May;88(5):1671-84
15829658
Soc Appl Bacteriol Symp Ser. 1990;19:167S-188S
2119063
J Biol Chem. 2001 Nov 23;276(47):43775-83
11562373
J Bacteriol. 2008 May;190(10):3646-57
18359813
Biochem Biophys Res Commun. 2004 Jan 23;313(4):856-62
14706621
Infect Immun. 1978 Apr;20(1):151-7
669789
Int J Food Microbiol. 2001 Sep 19;69(1-2):1-10
11589547