title: Improved base calling for the Illumina Genome Analyzer using machine learning strategies.
abstract: The Illumina Genome Analyzer generates millions of short sequencing reads. We present Ibis (Improved base identification system), an accurate, fast and easy-to-use base caller that significantly reduces the error rate and increases the output of usable reads. Ibis is faster and more robust with respect to chemistry and technology than other publicly available packages. Ibis is freely available under the GPL from http://bioinf.eva.mpg.de/Ibis/.
authors.affiliation: Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz, 04103 Leipzig, Germany. martin.kircher@eva.mpg.de
authors.forename: Martin
authors.initials: M
authors.lastname: Kircher
authors.forename: Udo
authors.initials: U
authors.lastname: Stenzel
authors.forename: Janet
authors.initials: J
authors.lastname: Kelso
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D016678:Genome; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
references.citation: BMC Bioinformatics. 2008 Oct 13;9:431
references.pmid: 18851737
references.citation: Bioinformatics. 2008 Mar 1;24(5):713-4
references.pmid: 18227114
references.citation: Nat Methods. 2008 Aug;5(8):679-82
references.pmid: 18604217
references.citation: Nature. 2008 Nov 6;456(7218):53-9
references.pmid: 18987734
references.citation: Genome Res. 1998 Mar;8(3):186-94
references.pmid: 9521922
references.citation: Nature. 2008 Mar 13;452(7184):215-9
references.pmid: 18278030