Nanopatterns biofunctionalized with cell adhesion molecule DM-GRASP offered as cell substrate: spacing determines attachment and differentiation of neurons.
The density/spacing of plasma membrane proteins is thought to be crucial for their function; clear-cut experimental evidence, however, is still rare. We examined nanopatterns biofunctionalized with cell adhesion molecule DM-GRASP with respect to their impact on neuron attachment and neurite growth. Data analysis/modeling revealed that these cellular responses improve with increasing DM-GRASP density, with the exception of one spacing which does not allow for the anchorage of a cytoskeletal protein (spectrin) to three DM-GRASP molecules.
Jaehrling
Steffen
S
Department of Developmental Neurobiology, Institute of Zoology, University of Heidelberg, 69120 Heidelberg, Im Neuenheimer Feld 232, Germany.
Thelen
Karsten
K
Wolfram
Tobias
T
Pollerberg
G Elisabeth
GE
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D003460:Crystallization / Q000379:methods; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*; D010316:Particle Size; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D046911:Macromolecular Substances; D019006:Neural Cell Adhesion Molecules