[Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC].
OBJECTIVE
To develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum.


METHODS
Agilent TC-C18 column (4.6 mm x 250 mm, 5 microm) was used at 30 degrees C with the mobile phase of methanol-water (75:25). The flow rate was set at 0.8 mL x min(-1). The detection wavelength was 321 nm.


RESULTS
The linear response ranged from 3.20-28.80 microg for +/- praeruptorin A (r = 0.9999, n = 5), 1.60-14.40 g for praeruptorin B (r = 0.9995, n = 5) and 1.64-14.76 g for qianhucoumarin E (r = 0.9994, n = 5), respectively. Recoveries were 98.92% with RSD 1.6% for praeruptorin A, 99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E.


CONCLUSIONS
The method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.
Wu
Wenling
W
Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
Chen
Jiajia
J
Liu
Shoujin
S
Yu
Nianjun
N
Fang
Chengwu
C
Zhang
Qingqing
Q
D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis* / Q000302:isolation & purification; D016014:Linear Models; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D013997:Time Factors
D003374:Coumarins; C030123:coumarin