Expression of bovine granulocyte chemotactic protein-2 (GCP-2) in neutrophils and a mammary epithelial cell line (MAC-T) in response to various bacterial cell wall components.
CXC chemokines are potential attractants for polymorphonuclear leucocytes (PMNs) and play an important role in resistance to infectious diseases, such as bovine mastitis. In this study, a bovine mammary epithelial cell line (MAC-T) and blood PMNs were stimulated with bacterial cell wall components of gram negative and gram positive bacteria, including lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN). The expression of two CXC chemokines, interleukin (IL)-8 and granulocyte chemotactic protein (GCP)-2 was analysed by real-time PCR. High concentrations (1 or 10 μg/mL) of LPS and LTA, but not PGN, significantly increased the expression of GCP-2 and IL-8 in both MAC-T and PMNs. Biopsies from mammary glands of cattle with clinical Escherichia coli mastitis also had increased expression of GCP-2. Using an in vitro transepithelial migration assay, recombinant human GCP-2 (rhGCP-2) showed weak chemoattractant effects on bovine blood PMNs. It was concluded that PMNs and MAC-T cells can express GCP-2 in response to certain bacterial cell components during the course of mastitis.
Yu
Chi
C
Department of Animal Science, National Pingtung University of Science and Technology, Neipu, Pingtung 91201, Taiwan.
Shi
Zhao-Ru
ZR
Chu
Chun-Yen
CY
Lee
Kuo-Hua
KH
Zhao
Xin
X
Lee
Jai-Wei
JW
D000818:Animals; D002417:Cattle; D002460:Cell Line; D054427:Chemokine CXCL6 / Q000097:blood*; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000097:blood; D004847:Epithelial Cells / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000097:blood; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D009504:Neutrophils / Q000378:metabolism*; D010457:Peptidoglycan / Q000096:biosynthesis / Q000097:blood; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000097:blood; D013682:Teichoic Acids / Q000096:biosynthesis / Q000097:blood
D054427:Chemokine CXCL6; D002630:Chemotactic Factors; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D011994:Recombinant Proteins; D013682:Teichoic Acids; C009900:lipoteichoic acid