Inducible-expression plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans.
We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.
Ind
Alice C
AC
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.
Porter
Steven L
SL
Brown
Mostyn T
MT
Byles
Elaine D
ED
de Beyer
Jennifer A
JA
Godfrey
Scott A
SA
Armitage
Judith P
JP
D002874:Chromosome Mapping; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial / Q000187:drug effects; D005822:Genetic Vectors; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007613:Kanamycin Resistance / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D010231:Paracoccus denitrificans / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012093:Replicon; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000235:genetics*
D007544:Isopropyl Thiogalactoside
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