Rapid on-chip genetic detection microfluidic platform for real world applications.
The development of genetic detection protocols for field applications is an important aspect of modern medical diagnostic technology and environmental monitoring. In this paper, we report a rapid, portable, and inexpensive DNA hybridization technique using a bead-based microfluidic platform that functions by passing fluorescently labeled target DNA through a chamber packed with functionalized beads within a microfluidic channel. DNA hybridization is then assessed using a digital camera attached to a Clare Chemical DR-45M dark reader non-UV transilluminator that uses visible light as an excitation source and a blue and amber filter to reveal fluorescence. This microfluidic approach significantly enhances hybridization by reducing the diffusion time between target DNA and the silica surface. The use of probe-functionalized beads as solid support also enhances the sensitivity and limit of detection due to a larger surface area per unit volume. This platform could be adapted for use in medical applications and environmental monitoring, including the detection of harmful organisms in the ballast water of ships.
Senapati
Satyajyoti
S
Mahon
Andrew R
AR
Gordon
Jason
J
Nowak
Carsten
C
Sengupta
Shramik
S
Powell
Thomas H Q
TH
Feder
Jeffrey
J
Lodge
David M
DM
Chang
Hsueh-Chia
HC
Lab Chip. 2006 Mar;6(3):414-21
16511625
Anal Chem. 1999 Nov 1;71(21):4851-9
10565276
Lab Chip. 2001 Dec;1(2):83-95
15100865
BMC Genomics. 2004 Sep 09;5:64
15357875
Clin Chim Acta. 2007 Sep;384(1-2):1-11
17689512
Lab Chip. 2005 Nov;5(11):1315-9
16234958
Nat Biotechnol. 2000 Jun;18(6):630-4
10835600
Anal Chem. 2004 Jun 15;76(12):3373-85
15193114
Anal Chim Acta. 2007 Jan 23;582(2):295-303
17386506
Mar Biotechnol (NY). 2005 Jan-Feb;7(1):11-20
15756474
Anal Chem. 2003 Jul 1;75(13):3079-85
12964754
Clin Chim Acta. 2006 Jan;363(1-2):206-20
16139259
Anal Chem. 2003 Sep 15;75(18):4732-9
14674448
Lab Chip. 2003 Nov;3(4):60N-68N
15007447
Anal Chem. 2003 Apr 15;75(8):1911-7
12713050