title: Constitutive expression of the ileS-2 gene responsible for high-level mupirocin resistance in Staphylococcus aureus.
abstract: Mupirocin is a topical antimicrobial agent that is used for the treatment of skin and postoperative wound infections, and the prevention of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA). However, the prevalence of mupirocin resistance in S. aureus, particularly in MRSA, has increased. High-level mupirocin resistance, with MICs >or=512 microg ml(-1), is mediated by the ileS-2 gene, which is located on conjugative plasmids. In the present study, we investigated whether mupirocin influences the expression of the ileS-2 gene responsible for high-level mupirocin resistance, and we present some evidence that this gene is not upregulated but constitutively expressed in S. aureus.
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Naira Elane Moreira de
authors.initials: NEM
authors.lastname: Oliveira
authors.affiliation: Instituto de Química, Bloco A, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Elisa D'Avila Costa
authors.initials: EDC
authors.lastname: Cavalcanti
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Marinella Silva
authors.initials: MS
authors.lastname: Laport
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Maria do Carmo de Freire
authors.initials: MDCF
authors.lastname: Bastos
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Marcia
authors.initials: M
authors.lastname: Giambiagi-deMarval
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D016712:Mupirocin / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D016712:Mupirocin