Inhibitory effect of Kangjia Pill on thyrocyte proliferation in rat goiter model.
OBJECTIVE
To investigate the inhibitory effects of Kangjia Pill (KJP) on the cell proliferation in rat goiter model induced by methimazole (MMI).


METHODS
Fifty-six Wistar rats were randomly divided into four groups: the normal group, MMI model group (MMI), low dose of KJP group (LKJP), and high dose of KJP (HKJP). Except the normal group (20 rats), the other groups (12 rats in each) were given 0.04% (w/v) MMI through the drinking water until the end of the experiment. One week later, the rats in the LKJP and HKJP groups were given KJP by gastrogavage at the dose of 250 mg/(kg x d) and 1,000 mg/(kg x d), respectively for 12 weeks. The relative thyroid weight (mg/100 g body weight) of each rat was accessed. The expression of proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry, and the correlation analysis between the PCNA positive thyrocytes and the relative thyroid weight was performed. The expressions of PCNA and cyclin D1 were examined with Western blotting.


RESULTS
After KJP treatment for 12 weeks, compared with the MMI group, the relative thyroid weight of the HKJP group decreased significantly, and the positive thyrocyte populations of PCNA in the two KJP groups reduced markedly (all P<0.05). The correlation analysis showed that PCNA was closely correlated with thyrocyte proliferation (r=0.685, P<0.05). KJP significantly decreased the protein expression of PCNA and cyclin D1 in the thyroid specimens (P<0.05), the high dose showed better effects.


CONCLUSIONS
KJP played a therapeutic role via inhibiting cell proliferation in the rat goitrous glands.
Han
Yong
Y
Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China.
Zhou
Jing
J
Yu
Shu-jing
SJ
Cui
Bin
B
Zhang
Hai-qing
HQ
Gao
Ling
L
Zhao
Jia-jun
JJ
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D006042:Goiter / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008713:Methimazole; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013607:Tablets; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*
C530106:Ccnd1 protein, rat; D004365:Drugs, Chinese Herbal; D018809:Proliferating Cell Nuclear Antigen; D013607:Tablets; C545319:kangjia; D019938:Cyclin D1; D008713:Methimazole
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