[Study on genomic imprinting of IGF2 in breast cancer].
OBJECTIVE
To explore the imprinting status of the insulin like growth factor-2 associated with the development of breast cancer.


METHODS
The samples of heterozygotes of IGF-2 was identified by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in 47 breast cancers tissue and their adjacent tissues, and then imprinting status of IGF-2 in these heterozygosis were detected by RT-PCR-RFLP.


RESULTS
Losses of imprinting of IGF2 were found in both of the cancer tissues and adjacent normal breast tissue and the incidence rates of losses of printing in the adjacent tissues(79.2%) were more higher than those in the cancer tissues (50.0%), it has statistical significance (P = 0.035). If there were losses of imprinting of IGF-2 in the breast cancer tissues, then there were losses of imprinting of IGF-2 in their corresponding adjacent tissues. But If there were maintain of imprinting of IGF-2 in the breast cancer tissues, then there were losses and maintain of imprinting of IGF-2 in their corresponding adjacent tissues.


CONCLUSIONS
The loss of imprinting of IGF-2 could be involved in the development of breast cancer.
Yang
Rong
R
Wuhan Medical Health Center of Maternal and Children, Wuhan 430016, China. yaoyang1217@yahoo.com.cn
Wang
Youjie
Y
Zhu
Xiaoling
X
Gao
Xiaohui
X
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018392:Genomic Imprinting; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
C035170:IGF2 protein, human; D007335:Insulin-Like Growth Factor II