Adipogenic differentiation is not influenced by lentivirus-mediated shRNA targeting the SOCS3 gene in adipose-derived stromal cells.
The adipose-derived stromal cells (ADSCs) are capable of adipogenic differentiation by which they can be used as the seed of adipocytes and as the model to study the mechanism of differentiation. Suppressor of cytokine signaling 3 (SOCS3), a negative regulator of leptin signaling, perhaps involves in cell survival and differentiation. However, to date, rare studies are performed on ADSCs differentiation and SOCS3 regulation. In this study, a lentiviral vector expressing a shRNA targeting SOCS3 gene (Lv-shSOCS3) is developed and tested. To observe the effect of Lv-shSOCS3 on ADSCs differentiation, we infected ADSCs with Lv-shSOCS3 or Lv-shSOCS3-NC (non-silenced control lentivirus) and examined adipocyte differentiation using morphology and the expression of key gene for adipogenic differentiation using real-time PCR. The results show that Lv-shSOCS3 can significantly suppress the expression of SOCS3 mRNA and protein in ADSCs. There are no distinguishable differences on adipogenic differentiation and the expression of peroxisome proliferators-activated receptor gamma (PPARgamma) mRNA for ADSCs between infection with Lv-shSOCS3 and Lv-shSOCS3-NC. We conclude that SOCS3 knock-down does not influence the growth and adipogenic differentiation feature of ADSCs. Our study provides a new molecular basis for understanding the differentiation mechanism of ADSCs and lays the foundation for further study of the biological functions of SOCS3.
Liu
Li
L
Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, No. 92 Beier Road, Heping District, Shenyang, Liaoning Province 110001, China. liuli@mail.cmu.edu.cn
Gu
Hailun
H
Yang
Jun
J
Ma
Shuang
S
Yu
Fei
F
Ren
Yahao
Y
An
Li
L
D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002460:Cell Line; D020868:Gene Silencing; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics* / Q000378:metabolism
D034741:RNA, Small Interfering; C491410:Socs3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D049452:Green Fluorescent Proteins
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