title: Early intestinal morphological changes following benzalkonium chloride treatment in a rat model of short bowel syndrome.
abstract: BACKGROUND
  The aim of this study was to assess early morphological changes of the residual small intestine 30 days after application of Benzalkonium Chloride (BAC) in a rat model of short bowel syndrome (SBS).
  
  
  METHODS
  Twenty nine Wistar rats (260 +/- 20 g) underwent 80% midsmall bowel resection with end to end anastomosis. In group 1 (n=14) BAC solution 0.1% was applied to a 2 cm segment of jejunum, and in group 2 (n=15) normal saline was applied in a similar manner and the rats were sacrificed 30 days after operation. Specimens for histological examination were obtained initially and at sacrifice.
  
  
  RESULTS
  In the BAC treated jejunal segment (group 1), a statistically significant increase (p< 0.05) was noted in villous height by 33.2%, in crypt depth by 26.4%, in muscle thickness by 26%, 109.6% in intestinal diameter, and 20% in total intestinal length, compared to group 2.
  
  
  CONCLUSIONS
  BAC application to the serosal surface of rat's jejunum in SBS is a simple method that within only 4 weeks can topically augment the natural adaptation process noticed following intestinal resection. Further research with a tapering technique performed in sequence is suggested, to prevent possible problems associated with pseudoobstruction in the long term.
authors.affiliation: 2nd Department of General Surgery, Evangelismos Hospital, Athens, Greece. katerinamichopoulou@hotmail.com
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Michopoulou
authors.forename: Basilis
authors.initials: B
authors.lastname: Triggas
authors.forename: Eleni
authors.initials: E
authors.lastname: Hra
authors.forename: Christina
authors.initials: C
authors.lastname: Spyratou
authors.forename: Anastasios
authors.initials: A
authors.lastname: Milonas
authors.forename: Despina
authors.initials: D
authors.lastname: Perrea
authors.forename: Ismini
authors.initials: I
authors.lastname: Donta
authors.forename: Stefanos
authors.initials: S
authors.lastname: Papadopoulos
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology* / Q000627:therapeutic use; D001548:Benzalkonium Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects; D003714:Denervation; D004195:Disease Models, Animal; D017615:Enteric Nervous System / Q000187:drug effects; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D008286:Malabsorption Syndromes / Q000517:prevention & control; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012778:Short Bowel Syndrome / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery
chemical_list: D000891:Anti-Infective Agents, Local; D001548:Benzalkonium Compounds