Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila.
Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.
Witsell
Alice
A
Department of Biology, Tufts University, Medford, Massachusetts 02155, USA.
Kane
Daniel P
DP
Rubin
Sarah
S
McVey
Mitch
M
D000818:Animals; D053903:DNA Breaks, Double-Stranded; D004265:DNA Helicases / Q000235:genetics*; D004260:DNA Repair; D004251:DNA Transposable Elements / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005298:Fertility / Q000235:genetics; D017353:Gene Deletion; D049750:Genome, Insect / Q000235:genetics; D008297:Male; D016254:Mutagenesis, Insertional / Q000379:methods*; D009154:Mutation
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D004265:DNA Helicases; C470639:blm protein, Drosophila
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