Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae).
Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. The leaf acetone, ethyl acetate, and methanol extracts of Aegle marmelos (Linn.) Correa ex Roxb, Andrographis lineata Wallich ex Nees, and Cocculus hirsutus (L.) Diels were tested for oviposition-deterrent, ovicidal, and repellent activities against Anopheles subpictus Grassi (Diptera: Culicidae). The percentage of effective oviposition repellency of 92.60 , 93.04, 95.20, 88.26, 92.80, 94.01, 95.77, 96.93, and 92.54 at 500 ppm and the lowest repellency of 47.14, 58.00, 56.52, 64.93, 71.09, 66.42, 50.62, 57.62, and 65.73 at 31.25 ppm in acetone, ethyl acetate, and methanol extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus, respectively. The oviposition activity index (OAI) value of acetone, ethyl acetate, and methanol extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus at 500 ppm were -0.86, -0.87, -0.90, -0.78, -0.87, -0.86, -0.91, -0.94, and -0.86 respectively. The OAI values revealed that the solvent plant extracts have deterrent effect, and they caused a remarkable negative response resulting in oviposition of very few eggs. Mean percent hatchability of the ovicidal activity was observed 24 h after treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. Mortality of 100% with ethyl acetate extract of Aegle marmelos, methanol extracts Aegle marmelos, Andrographis lineata, and Cocculus hirsutus were exerted at 1,000 ppm. The maximum repellent activity was observed at 500 ppm in methanol extracts of Aegle marmelos, Andrographis lineata, and ethyl acetate extract of Cocculus hirsutus, and the mean complete protection time ranged from 90 to 120 min with the different extracts tested. These results suggest that the leaf extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus have the potential to be used as an ideal ecofriendly approach for the control of the Anopheles subpictus. Therefore, this study provides first report on the oviposition, ovicidal, and repellent activities against malaria vector, Anopheles subpictus of plant extracts from Southern India.
Elango
G
G
Unit of Bioactive Natural Products, P.G & Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Tamil Nadu, India.
Bagavan
A
A
Kamaraj
C
C
Abduz Zahir
A
A
Abdul Rahuman
A
A
D045471:Aegle / Q000737:chemistry*; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D000852:Anopheles / Q000187:drug effects*; D031605:Cocculus / Q000737:chemistry*; D007194:India; D007302:Insect Repellents / Q000302:isolation & purification / Q000494:pharmacology*; D007306:Insecticides / Q000302:isolation & purification / Q000494:pharmacology*; D010058:Oviposition / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D016019:Survival Analysis
D007302:Insect Repellents; D007306:Insecticides; D010936:Plant Extracts
Eur Rev Med Pharmacol Sci. 2009 Mar-Apr;13(2):99-103
19499844
Nat Prod Res. 2004 Apr;18(2):117-22
14984083
Bioresour Technol. 2005 Nov;96(16):1749-57
16051081
Indian J Med Res. 1992 Jul;95:200-3
1398810
Parasitol Res. 2006 Sep;99(4):478-90
16642384
Parasitol Res. 2009 Apr;104(5):1163-71
19085005
Fitoterapia. 2002 Feb;73(1):28-31
11864760
J Am Mosq Control Assoc. 1992 Mar;8(1):11-7
1583482
Biosci Biotechnol Biochem. 2002 Dec;66(12):2751-4
12596883
J Med Entomol. 2006 Jan;43(1):113-9
16506457
J Am Mosq Control Assoc. 1998 Jun;14(2):204-9
9673924
Pest Manag Sci. 2009 Mar;65(3):241-8
19086001
J Med Entomol. 2004 Jul;41(4):691-8
15311462
Phytother Res. 2004 Apr;18(4):335-8
15162372
Parasitol Res. 2008 Apr;102(5):951-5
18176817
Bioresour Technol. 2001 Feb;76(3):287-8
11198185
Am J Trop Med Hyg. 2007 Dec;77(6 Suppl):69-78
18165477
Parasitol Res. 2009 Aug;105(2):507-12
19343365
Bioresour Technol. 2009 Apr;100(7):2284-8
19070480
Bioresour Technol. 2003 Sep;89(2):185-9
12699939
J Ethnopharmacol. 1997 Jun;57(1):29-34
9234162
J Am Mosq Control Assoc. 2006 Mar;22(1):126-30
16646335
J Insect Sci. 2005;5:15
16341247
Med Vet Entomol. 2001 Jun;15(2):126-31
11434545
Parasitol Res. 2008 May;102(6):1289-91
18278511
Southeast Asian J Trop Med Public Health. 1980 Sep;11(3):399-404
7003734
Parasitol Res. 2008 Apr;102(5):981-8
18176816
Parasitol Res. 2008 Jan;102(2):289-92
17989995
Parasitol Res. 2009 Jun;104(6):1381-8
19165502
J Ethnopharmacol. 2001 Jun;76(1):73-6
11378284
J Vector Ecol. 2003 Dec;28(2):234-40
14714673
Bioresour Technol. 2008 May;99(8):3066-70
17669645
Nature. 2005 Mar 10;434(7030):214-7
15759000
Parasitol Res. 2008 Aug;103(3):545-50
18500538
Parasitol Res. 2008 Aug;103(3):691-5
18528709
J Econ Entomol. 1976 Oct 15;69(5):655-8
9433
Southeast Asian J Trop Med Public Health. 2006 Sep;37(5):915-31
17333734
N Engl J Med. 2002 Jul 4;347(1):13-8
12097535
J Am Mosq Control Assoc. 2003 Dec;19(4):397-403
14710743
Am J Trop Med Hyg. 2004 Aug;71(2 Suppl):1-15
15331814
Med Vet Entomol. 1999 May;13(2):124-31
10484158
Parasitol Res. 2009 Jan;104(2):337-40
18818950
Mem Inst Oswaldo Cruz. 2003 Mar;98(2):223-6
12764438
Phytochemistry. 2001 Feb;56(4):331-4
11249096
J Agric Food Chem. 1999 Aug;47(8):3411-5
10552665
J Am Mosq Control Assoc. 1991 Jun;7(2):210-37
1680152
J Environ Biol. 2002 Jul;23(3):335-9
12597579