title: Reactive oxygen species activate the group IV muscle afferents in resting and exercising muscle in rats.
abstract: We tested the hypothesis that the reactive oxygen species (ROS) produced at rest and mostly during muscle contraction may stimulate the group IV muscle afferents. In rats, afferent activity was recorded in the peroneal nerve innervating the tibialis anterior muscle. Group IV afferents were identified from measurements of their conduction velocity and response to lactic acid. Comparing the group IV response to an intramuscular injection of buffered isotonic NaCl solution, we searched for the effects of a ROS donor (H2O2) or a ROS inhibitor (superoxide dismutase, SOD) on the baseline afferent activity in resting muscles. We also explored the consequences of a pre-treatment with SOD on the afferent nerve response to H2O2 injection or electrical muscle stimulation (MS). In other animals, we measured the changes in intramuscular level of a marker of oxidative stress (isoprostanes) after each test agent. H2O2 injection markedly activated all recorded group IV afferents. SOD injection lowered the baseline activity of 50 out of 70 afferent units, suppressed the afferent response to H2O2 injection, and delayed and reduced the MS-induced activation of all recorded units. Intramuscular isoprostanes level significantly increased after H2O2 injection or MS, the oxidative stress being absent in muscles pre-treated with SOD. We concluded that ROS influence both the spontaneous and contraction-induced activities of the group IV muscle afferents and are a potent stimulus of muscle metaboreceptors.
authors.affiliation: UMR MD2 P2COE, IFR Jean Roche, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Delliaux
authors.forename: Christelle
authors.initials: C
authors.lastname: Brerro-Saby
authors.forename: Jean Guillaume
authors.initials: JG
authors.lastname: Steinberg
authors.forename: Yves
authors.initials: Y
authors.lastname: Jammes
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004594:Electrophysiology; D016166:Free Radical Scavengers / Q000378:metabolism / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D016877:Oxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase
references.citation: J Physiol. 2005 Sep 1;567(Pt 2):641-50
references.pmid: 15975985
references.citation: Clin Exp Pharmacol Physiol. 1996 Aug;23(8):700-8
references.pmid: 8886494
references.citation: Ann N Y Acad Sci. 2001 Jun;940:74-95
references.pmid: 11458709
references.citation: J Physiol. 2006 Oct 1;576(Pt 1):279-88
references.pmid: 16840514
references.citation: Brain Res. 2004 Oct 15;1023(2):222-30
references.pmid: 15374748
references.citation: J Appl Physiol (1985). 2003 Oct;95(4):1476-84
references.pmid: 12819224
references.citation: Med Sci Sports Exerc. 2007 Jun;39(6):964-8
references.pmid: 17545886
references.citation: Neurosci Lett. 2001 May 18;304(1-2):45-8
references.pmid: 11335051
references.citation: J Appl Physiol (1985). 1999 Oct;87(4):1360-7
references.pmid: 10517764
references.citation: J Appl Physiol (1985). 1999 Aug;87(2):545-53
references.pmid: 10444611
references.citation: J Biol Chem. 1991 Feb 5;266(4):2354-61
references.pmid: 1846625
references.citation: J Peripher Nerv Syst. 1998;3(4):267-76
references.pmid: 10970127
references.citation: J Anat. 1969 Sep;105(Pt 2):231-54
references.pmid: 5802932
references.citation: Med Sci Sports Exerc. 2000 Sep;32(9):1576-81
references.pmid: 10994907
references.citation: Respir Physiol Neurobiol. 2002 Apr;130(2):189-99
references.pmid: 12380009
references.citation: J Neurophysiol. 1987 Jul;58(1):125-37
references.pmid: 3039078
references.citation: Am J Physiol Lung Cell Mol Physiol. 2003 Dec;285(6):L1184-9
references.pmid: 14604848
references.citation: Brain Res. 1997 Mar 7;750(1-2):147-54
references.pmid: 9098539
references.citation: Circ Res. 1987 Oct;61(4 Pt 2):I60-5
references.pmid: 3652404
references.citation: Muscle Nerve. 2000 Aug;23(8):1187-93
references.pmid: 10918254
references.citation: Pflugers Arch. 2009 Feb;457(4):877-84
references.pmid: 18846383
references.citation: Acta Physiol Scand. 1992 Jun;145(2):151-7
references.pmid: 1636444
references.citation: J Appl Physiol (1985). 1988 Jun;64(6):2306-13
references.pmid: 3136123
references.citation: Neurosci Lett. 1998 Nov 27;257(2):109-12
references.pmid: 9865939
references.citation: J Appl Physiol (1985). 1992 May;72(5):1631-48
references.pmid: 1601767
references.citation: Circ Res. 1994 May;74(5):895-903
references.pmid: 8156636
references.citation: Physiol Rev. 2000 Jul;80(3):1055-81
references.pmid: 10893431
references.citation: Am J Physiol Heart Circ Physiol. 2006 Jul;291(1):H210-5
references.pmid: 16772525