[Effects of deoxynivalenol on apoptosis of human gastric carcinoma cell line SGC-7901, BGC-823 in vitro].
OBJECTIVE
To explore the putative effects and possible mechanisms of deoxynivalenol (DON) on the apoptosis of human gastric carcinoma cell line SGC-7901, BGC-823 in vitro.


METHODS
SGC-7901 and BGC-823 cells were treated with DON (50, 100, 1000 microg/L) for 72 hours, and then cells were harvested for the studies of apoptosis and the expression of Bax and Bcl-2 with flow cytometry (FCM), immunocytochemical staining and Western blot.


RESULTS
FCM results showed that the apoptosis rates of SGC-7901 and BGC-823 cells in DON treatment groups were higher than that in control, and positive dose-effect correlations could be found in both cell lines (SGC-7901: r = 0.660, P < 0.05, n=4, BGC-823: r = 0.750, P < 0.01, n=4). FCM, immunocytochemical staining and Western blot results showed that the expression of Bax was increased while that of Bcl-2 was decreased in DON treatment groups of SGC-7901 and BGC-823.


CONCLUSIONS
The results suggested that DON could induce apoptosis of SGC-7901, BGC-823 cells in vitro in a dose-dependent manner, and possible mechanisms may be increased formation of Bax-Bax homology dimer and decreased formationof Bax-Bcl-2 dimer by up-regulation of the expression of Bax and down-regulation of that Bcl-2.
Liu
Jing
J
Laboratory of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, China.
Xing
Xin
X
Xing
Lingxiao
L
Zhou
Bingjuan
B
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*; D014255:Trichothecenes / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D000970:Antineoplastic Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D014255:Trichothecenes; D051028:bcl-2-Associated X Protein; C007262:deoxynivalenol