Photoregulation of thrombin aptamer activity using Bhc caging strategy.
Thrombin aptamer was attempted to cage with Bhc (6-bromo-7-hydroxycoumarin-4-ylmethyl) group for controlling its specific affinity to target molecular through photolysis. By multiple-caging strategy, aptamer could be rendered biologically inert and partially restored with subsequently illumination. This provides a convenient method for photoregulating aptamer activity with exact spatiotemporal resolution.
Li
Yiming
Y
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Shi
Jing
J
Luo
Zhaofeng
Z
Jiang
Hao
H
Chen
Xiaoyun
X
Wang
Fengliang
F
Wu
Xu
X
Guo
Qingxiang
Q
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D008027:Light; D010782:Photolysis; D011485:Protein Binding; D013917:Thrombin / Q000378:metabolism; D014468:Umbelliferones / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D014468:Umbelliferones; C081676:thrombin aptamer; C031477:7-hydroxycoumarin; D013917:Thrombin