ARMS2/HTRA1 and CFH polymorphisms are not associated with choroidal neovascularization in highly myopic eyes of the elderly Japanese population.
OBJECTIVE
The purpose of this study was to investigate whether the genetic risk factors of age-related macular degeneration (AMD) are associated with the development of choroidal neovascularization (CNV) in highly myopic eyes of elderly Japanese.


METHODS
Highly myopic elderly Japanese patients with and without CNV were genotyped for three AMD-associated single nucleotide polymorphisms (SNPs), namely rs10490924 (A69S) of ARMS2, rs11200638 of HTRA1, and rs1061170 (Y402H) of complement factor H (CFH), with the TaqMan SNP assay. One hundred and eighty-three unrelated highly myopic (axial lengths>26.00 mm or refractive errors>-6.0 diopters) Japanese patients with CNV who were >or=50 years of age (mean age+/-standard deviation of 62.7+/-6.3 years) and 170 highly myopic patients without CNV who were >or=50 years old (62.3+/-7.1 years) were studied. The differences in the genotypic distributions for the three SNPs between the two groups were tested with the Trend chi2 test, and logistic regression analyses were performed for age and gender adjustment.


RESULTS
No significant difference was detected in the distribution of the three SNPs, rs10490924 (P>0.1), rs11200638 (P>0.1), and rs1061170 (P>0.5), between the two groups even after adjustments for age and gender differences.


CONCLUSIONS
The genetic risk factors of AMD related to these SNPs do not contribute significantly to the development of CNV in a highly myopic elderly Japanese population.
Nakanishi
H
H
Department of Ophthalmology and Visual Sciences, Center for Genomic Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan. hideon@kuhp.kyoto-u.ac.jp
Gotoh
N
N
Yamada
R
R
Yamashiro
K
K
Otani
A
A
Hayashi
H
H
Tsujikawa
A
A
Shimada
N
N
Ohno-Matsui
K
K
Mochizuki
M
M
Saito
M
M
Saito
K
K
Iida
T
T
Matsuda
F
F
Yoshimura
N
N
D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D020256:Choroidal Neovascularization / Q000235:genetics*; D017242:Complement Factor H / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D000074289:High-Temperature Requirement A Serine Peptidase 1; D006801:Humans; D007564:Japan; D008268:Macular Degeneration / Q000150:complications / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009216:Myopia / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012697:Serine Endopeptidases / Q000235:genetics*
C514392:ARMS2 protein, human; D011506:Proteins; D017242:Complement Factor H; D000074289:High-Temperature Requirement A Serine Peptidase 1; C474012:HTRA1 protein, human; D012697:Serine Endopeptidases