Bovine serum albumin promotes IL-1beta and TNF-alpha secretion by N9 microglial cells.
Bovine serum albumin (BSA) is generally used in biomedical experiments. In the solution of some reagents, BSA is necessary to maintain the stability and concentration of the effective component. Therefore, the potential impact of BSA on experimental results should not be neglected when BSA is used. In this study, we observed that BSA induced significant upregulation of mRNA expression and release of pro-inflammatory cytokines, IL-1beta, and TNF-alpha, by N9 microglial cells. Our results suggest that the effects of BSA should be taken into account in experiments on microglia or the central nervous system when BSA is used. In light of the high similarity and homology among mammalian albumins, our findings also indicate that serum albumin may be a potent trigger of cytokine release by microglia.
Zhao
Tian-zhi
TZ
Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing, 400038, China.
Xia
Yong-zhi
YZ
Li
Lan
L
Li
Jian
J
Zhu
Gang
G
Chen
Shi
S
Feng
Hua
H
Lin
Jiang-kai
JK
D000818:Animals; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D012710:Serum Albumin, Bovine
J Neurochem. 2000 Dec;75(6):2309-15
11080182
Neuropathol Appl Neurobiol. 2007 Feb;33(1):86-98
17239011
J Neurochem. 2005 Mar;92(6):1363-76
15748155
Glia. 2005 Apr 1;50(1):21-31
15602748
J Neurotrauma. 1996 Oct;13(10):557-68
8915907
Methods. 2001 Dec;25(4):402-8
11846609
J Acquir Immune Defic Syndr (1988). 1994 Mar;7(3):265-71
7906304
N Engl J Med. 2007 Aug 30;357(9):874-84
17761591
Neurocrit Care. 2005;2(1):83-95
16174975
Glia. 1997 Dec;21(4):413-8
9419017
Neurology. 2007 May 22;68(21):1809-14
17515542
J Neuropathol Exp Neurol. 2002 Nov;61(11):992-1000
12430716
J Neuroimmune Pharmacol. 2006 Jun;1(2):127-37
18040779
Mol Pain. 2007 Nov 01;3:33
17974036
Glia. 2002 Nov;40(2):140-55
12379902
J Neurosci Res. 2005 Jun 1;80(5):707-14
15880558
Prog Brain Res. 2001;132:367-74
11545003
Prog Neurobiol. 2005 Jun;76(2):77-98
16081203
Nat Rev Neurosci. 2007 Jan;8(1):57-69
17180163
J Neurosci. 1997 Jan 15;17(2):615-24
8987784
J Am Soc Mass Spectrom. 2004 Aug;15(8):1237-47
15276171
Clin Diagn Lab Immunol. 1998 May;5(3):410-1
9606001
Fed Proc. 1976 Aug;35(10):2141-4
947793
Neurochem Int. 2005 Jul;47(1-2):152-8
15904993
Biol Pharm Bull. 2002 Aug;25(8):945-53
12186424
Int Immunopharmacol. 2005 Jan;5(1):185-93
15589480
Eur J Clin Invest. 2002 May;32(5):360-71
12027877
Mol Cell Neurosci. 2006 Jan;31(1):149-60
16297637