Quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time PCR.
The present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (PCR). Twenty patients with untreated chronic periodontitis were randomized into two groups. Before subgingival instrumentation and after 10 weeks samples in group A were taken first with a paper point half length (HP) of the probing depth, then with a paper point full length (FP) at the same site. In group B sampling sequence was reversed. Analysis by real-time PCR enabled quantification of six bacteria as well as total bacterial count (TBC). Statistical analysis included t test, Kappa, and Spearman's correlations. Higher TBC could be harvested by use of FP than by HP (mean of differences of ln-transformed counts before therapy: -0.791, CI [-1.515, -0.068], SD 0.770, p = 0.034; after therapy: -0.563, CI [-1.151, 0.024], SD 0.625, p = 0.059). The plaque composition regarding total target pathogens was similar for both samples. Both, for TBC as well as for single target bacteria a strong positive correlation was found between HP and FP (Kappa, Spearman correlation: Aggregatibacter actinomycetemcomitans 0.807, 0.778; Fusobacterium nucleatum 0.573, 0.772; Porphyromonas gingivalis 0.733, 0.824; Prevotella intermedia 0.480, 0.756; Treponema denticola 0.807, 0.814; and Tannerella forsythia 0.692, 0.695). The recovery of target pathogens was similar following sampling at various depths of the periodontal lesion.
Jervøe-Storm
Pia-Merete
PM
Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, Germany. storm@uni-bonn.de
AlAhdab
Hazem
H
Koltzscher
Max
M
Fimmers
Rolf
R
Jepsen
Søren
S
D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D058491:Bacterial Load; D001439:Bacteroides / Q000302:isolation & purification; D055113:Chronic Periodontitis / Q000382:microbiology* / Q000628:therapy; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000382:microbiology; D012534:Dental Scaling; D005260:Female; D005500:Follow-Up Studies; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000382:microbiology* / Q000628:therapy; D016133:Polymerase Chain Reaction / Q000379:methods*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000302:isolation & purification; D016745:Root Planing; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D013357:Subgingival Curettage; D048373:Treponema denticola / Q000302:isolation & purification
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