Relation of soluble endothelial protein C receptor and cytokines after allogeneic hematopoietic stem cell transplantation.
OBJECTIVE
The objective of this study was to elucidate the effects of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), interleukin 2 (IL-2), interleukin 6 (IL-6), and interleukin 8 (IL-8) on the expression of soluble endothelial protein C receptor (sEPCR) in the pathogenesis of thrombotic complications after hematopoietic stem cell transplantation (HSCT).


METHODS
The relationship between plasma concentrations of proinflammatory cytokines (TNF-α, IL-1β, IL-2, IL-6, and IL-8) and sEPCR was evaluated in 32 consecutive allogeneic hematopoietic stem cell-transplanted patients prior to conditioning regimen and randomly once between +5 and +30 days after transplantation and compared these results with 20 healthy controls.


RESULTS
Soluble endothelial protein C receptor levels did not indicate any significant difference between pre- and posttransplantation period, and sEPCR levels showed a significantly negative correlation between IL-6 and IL-8 (sEPCR and IL-6, r = -.43, P < .01; sEPCR and IL-8, r = -.57, P < .01). There was no correlation between sEPCR levels and TNF-α, IL-1β, or IL-2 (sEPCR and TNF-α, r = -.13, P > .05; sEPCR and IL-1β, r = -.1, P ≥ .05; sEPCR and IL-2, r = -.07, P > .05).


CONCLUSIONS
Our results suggest that the production of sEPCR was not affected by allogeneic HSCT. Soluble endothelial protein C receptor did not show any positive correlation between these proinflammatory cytokines (TNF-α, IL-1β, IL-2, IL-6, and IL-8), on the contrary a significantly negative correlation was determined between sEPCR and either IL-6 or IL-8. This negative correlation may be a protective mechanism in the pathway of protein C activation.
Azik
Fatih Mehmet
FM
Department of Pediatric Hematology, Ankara University School of Medicine, Ankara, Turkey. mfatihazik@yahoo.com
Ertem
Mehmet
M
Ileri
Talia
T
Ince
Elif Unal
EU
Uysal
Zumrut
Z
Egin
Yonca
Y
Akar
Nejat
N
D000293:Adolescent; D015703:Antigens, CD / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood*; D000074283:Endothelial Protein C Receptor; D005260:Female; D019337:Hematologic Neoplasms / Q000097:blood / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007223:Infant; D008297:Male; D011486:Protein C / Q000032:analysis; D011956:Receptors, Cell Surface / Q000097:blood*; D013927:Thrombosis / Q000097:blood* / Q000209:etiology; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous
D015703:Antigens, CD; D016207:Cytokines; D000074283:Endothelial Protein C Receptor; C478370:PROCR protein, human; D011486:Protein C; D011956:Receptors, Cell Surface