[Establishment of reconstituted human engineered skin model for evaluating cosmetic skin corrosion/irritation].
OBJECTIVE
Constructing reconstituted human engineered skin model, to establish an alternative approach for evaluating cosmetics skin corrosion and irritation.


METHODS
A cut of human dermal tissue was taken, and the epidermis and dermis were separated respectively for primary cell culture. The reconstituted skin models were onstructed with rat tail collagen. Then, the models under air-liquid face were cultured. Skin models were affected by Chemicals for 3 min or 1 h in corrosivity test, and the models were affected for 15 min in irritation test following with 42 h culture. Then, the cytoactive were detected by MTT essay and IL-1alpha releases level were detected by ELISA.


RESULTS
In corrosion test, the cytoactive was declined < 50% (3 min) and 15% (1 h) after giving three corrosive chemicals. However, the cytoactive wasn't change for two un-corrosive chemicals. In irritation test, three of four irritation chemicals caused cytoactive less than 50%, and another one made the IL-1alpha release more than 60 pg/ml in ELISA test. It seemed that all of four chemicals were irritations.


CONCLUSIONS
It was suggested that constructing reconstituted human engineered skin model could be established as an alternative approach for predicting skin corrosion and irritation as possible.
Liu
Zhen
Z
The Second Military Medicine University, Shanghai 200433, China. liuzhen5-1@163.com
Liu
Junping
J
Xu
Guifeng
G
Wang
Fei
F
D002478:Cells, Cultured; D003343:Corrosion; D003358:Cosmetics / Q000633:toxicity*; D020405:Dermis / Q000166:cytology / Q000187:drug effects; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D006801:Humans; D007509:Irritants / Q000145:classification / Q000633:toxicity; D008297:Male; D008954:Models, Biological; D010413:Penis; D012867:Skin / Q000166:cytology*; D023422:Skin Irritancy Tests / Q000379:methods*; D023822:Tissue Engineering
D003358:Cosmetics; D007509:Irritants