Glycine betaine enhances growth of nitrogen-fixing bacteria Gluconacetobacter diazotrophicus PAL5 under saline stress conditions.
In this study, the effect of glycine betaine as osmoprotectant compound for Gluconacetobacter diazotrophicus PAL5 was evaluated by kinetic growth parameters. Batch fermentation assays were performed employing media supplemented with different sodium chloride concentrations to simulate saline stress conditions. Salt concentrations of 50-300 mM led to decreased cell concentrations, while the maximum specific growth rates and cell productivities were reduced at concentrations above 100-mM NaCl. Salt inhibition was mainly observed in media with 200- and 300-mM NaCl, in which drastic changes in cell morphology were also noted. The addition of glycine betaine to the media showed to be efficient to counteract the salt inhibitory effect by increasing some fermentation parameters. However, the osmoprotectant was not able to revert the polymorphism promoted by higher salt concentrations.
Boniolo
Fabrízio Siqueira
FS
North Fluminense State University (UENF), Campos dos Goytacazes, RJ, Brazil.
Rodrigues
Raphael Cardoso
RC
Delatorre
Edson Oliveira
EO
da Silveira
Mauricio Moura
MM
Flores
Victor Martín Quintana
VM
Berbert-Molina
Marília Amorim
MA
D001622:Betaine / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D041821:Gluconacetobacter / Q000187:drug effects* / Q000254:growth & development; D009586:Nitrogen Fixation / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D014882:Water-Electrolyte Balance / Q000187:drug effects
D003470:Culture Media; D001622:Betaine; D012965:Sodium Chloride
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