Is there a link between protein kinase CK2 and auxin signaling?
Protein kinase CK2 is a pleitropic Ser/Thr kinase present in all eukaryotes. In order to study the effects of CK2 depletion on plant development, we have recently generated Arabidopsis transgenic plants expressing a CK2alpha-inactive mutant under the control of an inducible promoter. Our results showed that continuous expression of the transgene had a dominant negative effect and was lethal for Arabidopsis plants. Overexpression of the CK2alpha-inactive subunit provoked cell cycle arrest, by perturbation of both G(1)/S and G(2) cell cycle phases. The effects on cell division were particularly strong in root meristems, causing inhibition of lateral root formation even when the mutant protein was transiently induced. Processes that rely on cell expansion, such as hypocotyl elongation in dark-grown seedlings, were also strongly affected. We propose that CK2 regulates auxin-signaling pathways.
Moreno-Romero
Jordi
J
Departament de Bioquímica i Biologia Molecular; Universitat Autònoma de Barcelona; Bellaterra, Barcelona Spain.
Martínez
M Carmen
MC
Prog Nucleic Acid Res Mol Biol. 2003;74:239-73
14510078
Plant Physiol. 2001 Jan;125(1):396-405
11154347
Plant J. 2008 Jul;55(1):118-30
18363781
Nature. 2007 Oct 25;449(7165):1008-13
17960234
J Biol Chem. 1954 Dec;211(2):969-80
13221602
Mol Cell Biol. 1990 Aug;10(8):4089-99
2196445
Plant Cell. 2007 Mar;19(3):731-49
17337630
J Exp Bot. 2005 Dec;56(422):3183-92
16263904
Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9879-84
15159549
Biochem J. 2003 Jan 1;369(Pt 1):1-15
12396231
Plant J. 2004 Apr;38(2):332-47
15078335
Plant J. 1999 Sep;19(6):655-66
10571851
Cell. 2003 Nov 26;115(5):591-602
14651850
Plant Cell Physiol. 2006 Sep;47(9):1295-308
16926165
J Cell Sci. 2003 Apr 15;116(Pt 8):1563-77
12640040
Nature. 2005 Jan 6;433(7021):39-44
15635403
EMBO J. 2000 Sep 15;19(18):4997-5006
10990463