Evaluation of the free radical scavenging activity and radioprotective efficacy of Grewia asiatica fruit.
The radioprotective effect of Grewia asiatica fruit (GAE) which contains anthocyanin-type cyanidin 3-glucoside, vitamins C and A, minerals, carotenes and dietary fibre was studied. For the study Swiss albino mice were divided into five groups: (1) control (vehicle treated); (2) GAE treated (700 mg kg(-1) day(-1) for 15 days); (3) irradiated (5 Gy); (4) GAE+irradiated and (5) irradiated+GAE treated. The irradiation of animals resulted in a significant elevation of lipid peroxidation in terms of thiobarbituric acid reactive substances (TBARS) content and depletion in glutathione (GSH) and protein levels at all intervals studied, namely 1-30 days, in comparison to the control group. Treatment of mice with GAE before and after irradiation caused a significant depletion in TBARS content followed by a significant elevation in GSH and protein concentration in the intestine and testis of mice at all post-irradiation autopsy intervals in comparison to irradiated mice. Significant protection of DNA and RNA in testis was also noticed. GAE was found to have strong radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH(*)) and O(2)(-) assays and also showed in vitro radioprotective activity in protein carbonyl assay in a dose-dependent manner. The above results prove the radioprotective efficacy of GAE.
Sharma
Krishna V
KV
Radiation Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, Rajasthan, India.
Sisodia
Rashmi
R
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D016166:Free Radical Scavengers / Q000008:administration & dosage*; D005638:Fruit / Q000737:chemistry*; D032382:Grewia / Q000737:chemistry*; D051379:Mice; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000378:metabolism* / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000187:drug effects / Q000502:physiology; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D013481:Superoxides / Q000032:analysis*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*
D016166:Free Radical Scavengers; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides