Antifungal activity of organotin compounds with functionalized carboxylates evaluated by the microdilution bioassay in vitro.
We investigated the susceptibility of 96 well-characterized strains of yeast-like and filamentous fungi towards new organotin compounds: (1) [Sn(C4H9)3(OOCC6H4SO3H-2)], (2) Sn(C4H9)3{OOC(CH2)3P(C6H5)3}]Br, and (3) [Sn(C6H5)3[OOC(CH2)3N(CH3)3}]Cl. In the case of yeast-like fungi, the in vitro susceptibility tests were carried out according to the Clinical Laboratory Standards Institute (CLSI, formerly NCCLS) reference method M27-A2, while for filamentous fungi the investigations were conducted according to the M38-A and M38-P methods. The organotin complexes 1, 2 and 3 are active antifungal agents. Minimal inhibitory concentrations (MIC) were in the range of 0.25-4.68 microg/ml for all tested fungal strains. Considerably larger differences were found for minimal fungicidal concentrations (MFC). In the case of yeast-like fungi, the fungicidal effect was generally observed at organotin compounds concentrations of 2.34-9.37 microg/ml. The MFC values for filamentous fungi were considerably higher and were in the range of 18.74-50 microg/ml. In conclusion, organotin compounds 1, 2 and 3 showed high fungistatic and fungicidal activities against different species of pathogenic and nonpathogenic fungi. However, they were also highly cytotoxic towards two mammalian cell lines.
Dylag
Mariusz
M
Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw, Poland. mariusz.dylag@microb.uni.wroc.pl
Pruchnik
Hanna
H
Pruchnik
Florian
F
Majkowska-Skrobek
Grazyna
G
Ułaszewski
Stanisław
S
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests / Q000379:methods; D050296:Microbial Viability / Q000187:drug effects; D003904:Mitosporic Fungi / Q000187:drug effects*; D009947:Organotin Compounds / Q000494:pharmacology*; D012235:Rhodamines / Q000494:pharmacology; D015003:Yeasts / Q000187:drug effects*
D000935:Antifungal Agents; D009947:Organotin Compounds; D012235:Rhodamines; C022027:lissamine rhodamine B