title: Effects of glutathione on amyloid fibrillation of hen egg-white lysozyme.
abstract: This study examined the effects of glutathione on the fibrillation of hen egg-white lysozyme. We found that the fibrillation of lysozyme was considerably reduced by GSH while no anti-aggregating activity was detected with only GSSG. SDS-PAGE results also revealed that the addition of GSH led to an early occurrence of prominent lysozyme hydrolysis. Moreover, GSH was effective in inhibiting lysozyme fibrillation when GSH was added within 6 days of incubation. We conclude that the attenuation of lysozyme fibrillation is strongly dependent upon the redox environment. Our data may contribute to decipher the molecular mechanism of amyloid fibrillation.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan. sswang@ntu.edu.tw
authors.forename: Steven S-S
authors.initials: SS
authors.lastname: Wang
authors.forename: Shang-Wei
authors.initials: SW
authors.lastname: Chou
authors.forename: Kuan-Nan
authors.initials: KN
authors.lastname: Liu
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Wu
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D004220:Disulfides / Q000737:chemistry; D004586:Electrophoresis; D005978:Glutathione / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D008854:Microscopy, Electron; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D013057:Spectrum Analysis; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors
chemical_list: D000682:Amyloid; D004220:Disulfides; C421864:hen egg lysozyme; D009113:Muramidase; D005978:Glutathione