[Study of effects of procyanidin on the pathway of PPARgamma-NF-kappaB of thoracic aorta of hyperlipemia rats].
OBJECTIVE
To observe the effects of grape seed proanthocynadin extract (GSPE) on the expressions of PPARgamma mRNA and the activities of NF-kappaB of thoracic aorta of hyperlipemia rats.


METHODS
Four groups were randomly selected from 32 male Sprague-Dawley rats: control group (C), the hyperlipemia group (HL highlipid diets), the pioglitazone group (PI highlipid diets), the procyanidins group (PC highlipid diets). The PI is positive to PC. At the same time, rats were treated with either 10 mg/kg body weight pioglitazone, 100 mg/kg body weight procyanidins or distilled water by gavage daily till the end of the experiment. At 0 week, 3th week, 6th week, blood was gotten by tail vein. Rats were sacrificed at 6th week. The levels of serum TG, TC, HDL-C were tested by kits, PPARgamma mRNA were measured by RT-PCR and the activities of NF-kappaB by EMSA.


RESULTS
(1) At the 6th week, the levels of TG, TC in HL rats is higher than C rats (P < 0.05). Procyanidins and reduced the levels of TG, TC (P < 0.05), but there has no statistical difference between PI, C and PC. (2) Compared with C rats, the levels of PPARgamma mRNA of thoracic aorta were lower,the activities of the NF-kappaB of thoracic aorta were higher (P < 0.05) in HL and showed to be statistical difference (P < 0.05). (3) Compared with HL rats, the levels of PPARgamma mRNA of thoracic aorta were improved were reduced in PI rats (P < 0.05). (4) Compared with HL rats, the levels of PPARgamma mRNA of thoracic aorta were improved and the activities of the NF-kappaB of thoracic aorta were reduced in PC rats (P < 0.05).


CONCLUSIONS
PC can reduce the levels of TG, TC and PC can reduce the activities of NF-kappaB of thoracic aorta of hyperlipemia rats by preventing the expressions of PPARgamma mRNA downregulating.
Yin
Ruilan
R
Biochemistry and Molecular Biology Department of Shanxi Medical University, Taiyuan 030001, China. yrlpiao2005@yahoo.com.cn
Li
Xiaohan
X
Song
Ming
M
Wang
Huizhen
H
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism*; D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D004041:Dietary Fats / Q000008:administration & dosage; D015536:Down-Regulation; D006949:Hyperlipidemias / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D044945:Proanthocyanidins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D044946:Biflavonoids; D004041:Dietary Fats; D016328:NF-kappa B; D047495:PPAR gamma; D044945:Proanthocyanidins; D012333:RNA, Messenger; C017674:procyanidin; D002392:Catechin