Nuclear pore complex component MOS7/Nup88 is required for innate immunity and nuclear accumulation of defense regulators in Arabidopsis.
Plant immune responses depend on dynamic signaling events across the nuclear envelope through nuclear pores. Nuclear accumulation of certain resistance (R) proteins and downstream signal transducers are critical for their functions, but it is not understood how these processes are controlled. Here, we report the identification, cloning, and analysis of Arabidopsis thaliana modifier of snc1,7 (mos7-1), a partial loss-of-function mutation that suppresses immune responses conditioned by the autoactivated R protein snc1 (for suppressor of npr1-1, constitutive 1). mos7-1 single mutant plants exhibit defects in basal and R protein-mediated immunity and in systemic acquired resistance but do not display obvious pleiotropic defects in development, salt tolerance, or plant hormone responses. MOS7 is homologous to human and Drosophila melanogaster nucleoporin Nup88 and resides at the nuclear envelope. In animals, Nup88 attenuates nuclear export of activated NF-kappaB transcription factors, resulting in nuclear accumulation of NF-kappaB. Our analysis shows that nuclear accumulation of snc1 and the defense signaling components Enhanced Disease Susceptibility 1 and Nonexpresser of PR genes 1 is significantly reduced in mos7-1 plants, while nuclear retention of other tested proteins is unaffected. The data suggest that specifically modulating the nuclear concentrations of certain defense proteins regulates defense outputs.
Cheng
Yu Ti
YT
Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Germain
Hugo
H
Wiermer
Marcel
M
Bi
Dongling
D
Xu
Fang
F
García
Ana V
AV
Wirthmueller
Lennart
L
Després
Charles
C
Parker
Jane E
JE
Zhang
Yuelin
Y
Li
Xin
X
D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000502:physiology*; D015151:Immunoblotting; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D044224:Pseudomonas syringae / Q000472:pathogenicity
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C118837:EDS1 protein, Arabidopsis; C104835:NUP88 protein, human; D028861:Nuclear Pore Complex Proteins; C482739:SNC1 protein, Arabidopsis; C491301:mbo protein, Drosophila
Curr Opin Plant Biol. 2003 Aug;6(4):365-71
12873532
Cell. 2002 Mar 22;108(6):743-54
11955429
Plant Mol Biol. 2000 Apr;42(6):819-32
10890530
Mol Cell Biol. 2004 Mar;24(6):2373-84
14993277
Curr Opin Plant Biol. 2004 Aug;7(4):391-9
15231261
Curr Biol. 2005 Jun 21;15(12):1129-35
15964279
J Cell Sci. 1997 Apr;110 ( Pt 7):911-25
9133678
Plant J. 1999 Dec;20(6):695-705
10652141
Science. 1997 Dec 12;278(5345):1963-5
9395402
Plant J. 2007 Oct;52(1):82-93
17655649
Biochem Biophys Res Commun. 2008 Sep 26;374(3):424-30
18621024
Mol Plant Microbe Interact. 1995 Jul-Aug;8(4):637-40
8589418
Curr Biol. 2007 Dec 4;17(23):2023-9
17997306
Cell. 2003 Jun 27;113(7):935-44
12837250
Plant Cell. 1994 Jul;6(7):959-965
12244262
Mol Plant Microbe Interact. 1996 Jan;9(1):55-61
8589423
EMBO J. 2001 Oct 1;20(19):5400-11
11574472
Genome Biol. 2006;7(4):212
16677430
Plant J. 1998 Dec;16(6):735-43
10069079
Plant Cell. 2005 Sep;17(9):2601-13
16040633
Cell. 2005 Jun 3;121(5):749-59
15935761
Science. 2007 Feb 23;315(5815):1098-103
17185563
Cell. 2003 Feb 7;112(3):379-89
12581527
Cell. 1997 Jan 10;88(1):57-63
9019406
Plant Cell. 2003 Nov;15(11):2636-46
14576290
Genes Dev. 2000 Aug 1;14(15):1945-57
10921908
Curr Opin Plant Biol. 2005 Aug;8(4):383-9
15939664
Plant J. 2004 Oct;40(2):225-37
15447649
Plant Physiol. 2002 Jun;129(2):440-50
12068090
Annu Rev Plant Biol. 2003;54:23-61
14502984
Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2122-7
9122158
Nat Protoc. 2007;2(7):1565-72
17585298
Cell. 1999 Aug 6;98(3):329-39
10458608
Immunity. 2006 Mar;24(3):295-304
16546098
Plant Cell. 2007 Dec;19(12):4061-76
18065690
Proc Natl Acad Sci U S A. 1999 May 25;96(11):6523-8
10339621
Plant Cell. 2006 Jul;18(7):1590-603
16751346
Plant Cell. 2005 Apr;17(4):1306-16
15772285
Plant Cell. 2003 Nov;15(11):2647-53
14576289
J Cell Biol. 2003 Nov 24;163(4):701-6
14638854
PLoS Biol. 2007 Mar;5(3):e68
17298188
Mol Plant Microbe Interact. 2001 Oct;14(10):1131-9
11605952
Science. 1995 Aug 11;269(5225):843-6
7638602
Mol Cell Biol. 2006 Dec;26(24):9533-43
17030626
Genes Dev. 2007 Jun 15;21(12):1484-93
17575050
Cell. 2003 Feb 7;112(3):369-77
12581526
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10306-11
9707643
Plant J. 2002 Feb;29(4):439-51
11846877
J Cell Sci. 2006 Nov 1;119(Pt 21):4409-19
17032737
Plant Cell. 2007 May;19(5):1682-94
17526750
Science. 2008 Aug 15;321(5891):952-6
18635760
Science. 2007 Nov 30;318(5855):1412-6
18048681
Nature. 2006 Nov 16;444(7117):323-9
17108957
Plant Cell. 1994 Nov;6(11):1583-1592
12244227
Plant Physiol. 2001 Dec;127(4):1466-75
11743090
Annu Rev Phytopathol. 2004;42:185-209
15283665
Mol Cell Biol. 1997 Feb;17(2):906-20
9001245