Protein versus DNA as a marker for peripheral blood mononuclear cell counting.
Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 x 10(6) to 300 x 10(6) PBMCs/mL.
Jansen
Robert S
RS
Department of Pharmacy & Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, 1066, EC, Amsterdam, The Netherlands. robert.jansen@slz.nl
Rosing
Hilde
H
Schellens
Jan H M
JH
Beijnen
Jos H
JH
D004247:DNA / Q000032:analysis*; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D016014:Linear Models; D011506:Proteins / Q000032:analysis*
D011506:Proteins; D004247:DNA
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