Amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation for hydrolysis of Cassava starch.
The optimization of process parameters for high amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation was carried out using central composite design. Finally, the optimal parameters obtained with the response surface methodology (RSM) were moisture 610.0 ml/kg, inoculum 30.0 ml (OD(600 nm) = 20.0)/kg, the amount ratio of wheat bran to rice husk 0.42, cassava starch concentration 20.0 g/kg, temperature 28 degrees C, and natural pH. Under the optimized conditions, 4,296 U/g of dry substrate of amylase activity was reached in the solid-state fermentation culture of the yeast strain A11 within 160 h, whereas the predicted maximum amylase activity of 4,222 U/g of dry substrate of amylase activity was derived from the RSM regression. It was found that cassava starch can be actively converted into monosaccharides and oligosaccharides by the crude amylase.
Chen
Lei
L
Key Laboratory of Marine Genetics and Gene Resource Exploitation (Ministry of Education), Ocean University of China, Yushan Road, No.5, Qingdao, China.
Chi
Zhen-Ming
ZM
Chi
Zhe
Z
Li
Mei
M
D000681:Amylases / Q000096:biosynthesis*; D000704:Analysis of Variance; D005285:Fermentation; D006868:Hydrolysis; D002365:Manihot / Q000737:chemistry*; D015203:Reproducibility of Results; D012440:Saccharomyces / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*
D013213:Starch; D000681:Amylases