Differentiation of Cryptococcus neoformans varieties and Cryptococcus gattii using CAP59-based loop-mediated isothermal DNA amplification.
Members of the Cryptococcus species complex (C. neoformans and C. gattii) are opportunistic pathogens responsible for frequently fatal cases of meningoencephalitis. These yeasts have been classified into five serotypes. Serotypes A and D are assigned to C. neoformans var. grubii and C. neoformans var. neoformans, respectively, Serotype AD strains are hybrids and serotype B and C strains are considered to belong to the related but distinct species C. gattii. Previous studies have identified 'serotype-associated' alleles of several genes in the Cryptococcus species complex. We developed a loop-mediated isothermal DNA amplification method using CAP59 allele-specific primers to identify the serotypes A, D and B/C of the Cryptococcus species complex.
Lucas
S
S
Institute of Hygiene and Tropical Medicine, New University of Lisbon, 1349-008 Lisbon.
da Luz Martins
M
M
Flores
O
O
Meyer
W
W
Spencer-Martins
I
I
Inácio
J
J
D000483:Alleles; D003453:Cryptococcosis / Q000382:microbiology; D056285:Cryptococcus gattii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D009172:Mycology / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
D005656:Fungal Proteins