Association between interleukin 1 gene cluster polymorphisms and bilateral distal interphalangeal osteoarthritis.
OBJECTIVE
To examine the association of the interleukin 1 gene (IL1) cluster polymorphisms and their haplotypes with bilateral distal interphalangeal joint osteoarthritis (DIP OA).


METHODS
Radiographs of both hands of 295 dentists and 248 teachers were examined and classified for the presence of OA using reference images. Bilateral DIP OA was defined by the presence of radiographic findings of grade 2 or more in at least 1 symmetrical pair of the DIP joints. We genotyped 10 single-nucleotide polymorphisms (SNP) in the IL1R1, IL1RL2, IL1A, IL1B, and IL1RN genes using polymerase chain reaction-based methods. Haplotypes were statistically reconstructed using the PHASE program. The association between the genotypes/diplotypes and bilateral DIP OA was examined with logistic regression analysis.


RESULTS
Two IL1B SNP (rs1143634 and rs1143633) were associated with bilateral DIP OA. The carriers of the IL1B rs1143634 minor allele had an increased OA risk [odds ratio (OR) 1.6; 95% confidence interval (CI) 1.08-2.26] compared to the noncarriers. The association was stronger in the dentists. The distribution of the IL1B rs1143633 genotype fit a recessive mode of inheritance (OR 3.03, 95% CI 1.35-6.83, p = 0.006). Two IL1B-IL1RN extended haplotype alleles (211-1 and 121-1) were associated with bilateral DIP OA. An interaction between the IL1B rs1143634 and the IL1R1-IL1RL2 and IL1B-IL1RN extended haplotypes and occupation (increased risk of OA among dentists only) was observed.


CONCLUSIONS
Our results provide further evidence for the role of IL1 gene cluster polymorphisms in the etiology of OA and suggest that some of these may predispose DIP joints to the effects of mechanical overload.
Solovieva
Svetlana
S
Centre of Expertise Health and Work Ability, Finnish Institute of Occupational Health, Helsinki, Finland. Svetlana.Solovieva@ttl.fi
Kämäräinen
Olli-Pekka
OP
Hirvonen
Ari
A
Hämäläinen
Satu
S
Laitala
Mari
M
Vehmas
Tapio
T
Luoma
Katariina
K
Näkki
Annu
A
Riihimäki
Hilkka
H
Ala-Kokko
Leena
L
Männikkö
Minna
M
Leino-Arjas
Päivi
P
D001696:Biomechanical Phenomena; D003813:Dentistry; D005260:Female; D005384:Finger Joint / Q000000981:diagnostic imaging / Q000503:physiopathology*; D005387:Finland; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D016015:Logistic Models; D008875:Middle Aged; D005810:Multigene Family / Q000235:genetics*; D009784:Occupational Diseases / Q000235:genetics / Q000503:physiopathology; D010003:Osteoarthritis / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011859:Radiography; D017472:Receptors, Interleukin-1 / Q000235:genetics; D012307:Risk Factors; D013663:Teaching; D016474:Weight-Bearing / Q000502:physiology
D007375:Interleukin-1; D017472:Receptors, Interleukin-1