title: Expressed sequence tags from the flower pathogen Claviceps purpurea.
abstract: SUMMARY The ascomycete Claviceps purpurea (ergot) is a biotrophic flower pathogen of rye and other grasses. The deleterious toxic effects of infected rye seeds on humans and grazing animals have been known since the Middle Ages. To gain further insight into the molecular basis of this disease, we generated about 10 000 expressed sequence tags (ESTs)-about 25% originating from axenic fungal culture and about 75% from tissues collected 6-20 days after infection of rye spikes. The pattern of axenic vs. in planta gene expression was compared. About 200 putative plant genes were identified within the in planta library. A high percentage of these were predicted to function in plant defence against the ergot fungus and other pathogens, for example pathogenesis-related proteins. Potential fungal pathogenicity and virulence genes were found via comparison with the pathogen-host interaction database (PHI-base; http://www.phi-base.org) and with genes known to be highly expressed in the haustoria of the bean rust fungus. Comparative analysis of Claviceps and two other fungal flower pathogens (necrotrophic Fusarium graminearum and biotrophic Ustilago maydis) highlighted similarities and differences in their lifestyles, for example all three fungi have signalling components and cell wall-degrading enzymes in their arsenal. In summary, the analysis of axenic and in planta ESTs yielded a collection of candidate genes to be evaluated for functional roles in this plant-microbe interaction.
authors.affiliation: Institut für Botanik, Westf. Wilhelms Universität Münster, Schlossgarten 3, D 48149 Münster, Germany. oser@uni-muenster.de
authors.forename: Birgitt
authors.initials: B
authors.lastname: Oeser
authors.forename: François
authors.initials: F
authors.lastname: Beaussart
authors.forename: Thomas
authors.initials: T
authors.lastname: Haarmann
authors.forename: Nicole
authors.initials: N
authors.lastname: Lorenz
authors.forename: Eva
authors.initials: E
authors.lastname: Nathues
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Rolke
authors.forename: Jan
authors.initials: J
authors.lastname: Scheffer
authors.forename: January
authors.initials: J
authors.lastname: Weiner
authors.forename: Paul
authors.initials: P
authors.lastname: Tudzynski
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002967:Claviceps / Q000235:genetics* / Q000472:pathogenicity; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics / Q000382:microbiology*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D017343:Genes, Plant; D054884:Host-Pathogen Interactions / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012434:Secale / Q000235:genetics / Q000382:microbiology*; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger
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