title: Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1H-NMR micro-flow probe.
abstract: A standard operating procedure (SOP) is presented for high-throughput metabonomics studies using a 600 MHz NMR spectrometer equipped with a micro-flow probe that is connected to an auto-sampler. The procedure is designed to minimize random and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the data acquired by a given NMR spectroscopist to ensure that (i) all researchers involved in the NMR data acquisition of a metabonomics research program perform equally and (ii) operator-associated variation of NMR data collection is statistically not relevant for the interpretation of results obtained from multivariate data analyses.
authors.affiliation: Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY, USA.
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Sukumaran
authors.forename: Erwin
authors.initials: E
authors.lastname: Garcia
authors.forename: Jia
authors.initials: J
authors.lastname: Hua
authors.forename: Walter
authors.initials: W
authors.lastname: Tabaczynski
authors.forename: Kunle
authors.initials: K
authors.lastname: Odunsi
authors.forename: Chris
authors.initials: C
authors.lastname: Andrews
authors.forename: Thomas
authors.initials: T
authors.lastname: Szyperski
mesh_terms: D019411:Clinical Laboratory Techniques / Q000295:instrumentation* / Q000592:standards*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D008832:Microchemistry; D015999:Multivariate Analysis; D044967:Serum / Q000737:chemistry*