title: Quercetin-3-O-beta-d-glucuronopyranoside (QGC)-induced HO-1 expression through ERK and PI3K activation in cultured feline esophageal epithelial cells.
abstract: Heme oxygenase-1 (HO-1) is one of the antioxidant enzymes which help protect against cellular damage. The present study examined the ability of Quercetin-3-O-beta-D-glucuronopyranoside (QGC), flavonoid glucoside extracted from Rumex Aquaticus Herba, to induce expression of HO-1 and analyzed its signaling mechanism in cultured feline esophageal epithelial cells (EEC). Culture of the esophageal epithelial cells from cat was prepared. The data suggested that QGC could result in enhanced antioxidant enzyme defense system via HO-1 expression and Nrf2 translocation involving both the ERK and PI3K-Akt pathways as well as partly PKC pathways in EEC.
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul 156-756, South Korea.
authors.forename: Ji Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Song
authors.forename: Sung Kwon
authors.initials: SK
authors.lastname: Ko
authors.forename: Wan Kyunn
authors.initials: WK
authors.lastname: Whang
authors.forename: Uy Dong
authors.initials: UD
authors.lastname: Sohn
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002415:Cats; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D004947:Esophagus / Q000187:drug effects / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006027:Glycosides / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D031804:Rumex / Q000737:chemistry*; D015398:Signal Transduction
chemical_list: D000975:Antioxidants; D006027:Glycosides; D051267:NF-E2-Related Factor 2; D010936:Plant Extracts; C443401:quercetin 3-O-glucuronide; D011794:Quercetin; D051547:Heme Oxygenase-1; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D011493:Protein Kinase C; D048049:Extracellular Signal-Regulated MAP Kinases