title: Plant TPX2 and related proteins.
abstract: At the onset of mitosis, microtubules form a bipolar spindle around the prophase nucleus. TPX2 is phosphorylated during mitosis and acts as a spindle assembly factor that nucleates microtubules in the close vicinity of chromosomes, independent of the centrosomes. Furthermore, it activates the kinase Aurora A and targets the Xenopus kinesin-like protein 2 to spindle poles. We have characterized the plant orthologue of TPX2 that possesses all identified functional domains of its animal counterpart. Moreover, we have demonstrated that it is exported before nuclear envelope breakdown and that its activity around the nuclear envelope is essential for prospindle assembly. Here, we compare the sequences of several characterized TPX2 domains, allowing us to define TPX2. We propose that true TPX2 orthologues share simultaneously all these conserved domains and that other proteins possessing only some of these functional blocks may be considered as TPX2-related proteins.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire Propre du CNRS (UPR 2357) Conventionné avec l'Université Louis Pasteur (Strasbourg I), Strasbourg, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Evrard
authors.forename: Laurent
authors.initials: L
authors.lastname: Pieuchot
authors.forename: Jan W
authors.initials: JW
authors.lastname: Vos
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vernos
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Schmit
references.citation: Nucleic Acids Res. 2003 Jul 1;31(13):3503-6
references.pmid: 12824354
references.citation: Brief Bioinform. 2002 Sep;3(3):265-74
references.pmid: 12230035
references.citation: Plant J. 2007 Mar;49(6):961-71
references.pmid: 17319849
references.citation: EMBO J. 2003 May 1;22(9):2060-70
references.pmid: 12727873
references.citation: Plant Physiol. 2008 Nov;148(3):1283-94
references.pmid: 18805954
references.citation: Cell. 2001 Jan 12;104(1):83-93
references.pmid: 11163242
references.citation: Plant Cell. 2008 Oct;20(10):2783-97
references.pmid: 18941054
references.citation: Mol Biol Cell. 2004 Dec;15(12):5318-28
references.pmid: 15385625
references.citation: Adv Appl Bioinform Chem. 2008;1:51-69
references.pmid: 21918606
references.citation: J Cell Sci. 2003 Dec 1;116(Pt 23):4791-8
references.pmid: 14600264
references.citation: Mol Cell. 2003 Oct;12(4):851-62
references.pmid: 14580337
references.citation: Cell Cycle. 2004 Apr;3(4):404-7
references.pmid: 14752279
references.citation: J Cell Biol. 2000 Jun 26;149(7):1405-18
references.pmid: 10871281
references.citation: Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W37-40
references.pmid: 15215345
references.citation: Dev Cell. 2005 Aug;9(2):237-48
references.pmid: 16054030
references.citation: Mol Cell. 2005 Aug 19;19(4):431-2
references.pmid: 16109366
references.citation: Science. 2006 Jun 9;312(5779):1491-5
references.pmid: 16627697
references.citation: EMBO J. 2008 Oct 8;27(19):2567-79
references.pmid: 18756265