Synthesis of PHB nanoparticles from optimized medium utilizing dairy industrial waste using Brevibacterium casei SRKP2: a green chemistry approach.
Polyhydroxyalkanoates (PHAs) are natural, biodegradable polymers accumulated by bacteria under nutritional exhausted condition where carbon source is in excess. A gram positive bacterium (designated strain SRKP2) that potentially accumulated polyhydroxybutyrate (PHB) was isolated from dairy industrial waste. From its morphological and physiological properties and nucleotide sequence of its 16S rRNA, it was suggested that strain SRKP2 was similar to Brevibacterium casei. PHAs were synthesized from a medium containing dairy waste, yeast extract and sea water. The synthesized PHAs were characterized by FT-IR as Polyhydroxybutyrate (PHB). Response surface methodology was applied to optimize the production of PHB. From the optimized medium the yield of PHB was found to be 2.940 g/L. Here we report the direct use of dairy waste and sea water as potential sources for the production of PHB. Produced PHB was used to synthesize nanoparticles using solvent displacement technique.
Ram Kumar Pandian
Sureshbabu
S
Department of Biotechnology, Division of Cellular and Molecular Biology, Kalasalingam University, Anand Nagar, Krishnankoil 626190, Tamil Nadu, India.
Deepak
Venkatraman
V
Kalishwaralal
Kalimuthu
K
Muniyandi
Jeyaraj
J
Rameshkumar
Neelamegam
N
Gurunathan
Sangiliyandi
S
D000704:Analysis of Variance; D001951:Brevibacterium / Q000166:cytology / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism*; D003612:Dairying; D055772:Green Chemistry Technology / Q000379:methods*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007220:Industrial Waste; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry / Q000382:microbiology*; D011091:Polyesters / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D003470:Culture Media; D006885:Hydroxybutyrates; D007220:Industrial Waste; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates