title: Computational analyses of mammalian lactate dehydrogenases: human, mouse, opossum and platypus LDHs.
abstract: Computational methods were used to predict the amino acid sequences and gene locations for mammalian lactate dehydrogenase (LDH) genes and proteins using genome sequence databanks. Human LDHA, LDHC and LDH6A genes were located in tandem on chromosome 11, while LDH6B and LDH6C genes were on chromosomes 15 and 12, respectively. Opossum LDHC and LDH6B genes were located in tandem with the opossum LDHA gene on chromosome 5 and contained 7 (LDHA and LDHC) or 8 (LDH6B) exons. An amino acid sequence prediction for the opossum LDH6B subunit gave an extended N-terminal sequence, similar to the human and mouse LDH6B sequences, which may support the export of this enzyme into mitochondria. The platypus genome contained at least 3 LDH genes encoding LDHA, LDHB and LDH6B subunits. Phylogenetic studies and sequence analyses indicated that LDHA, LDHB and LDH6B genes are present in all mammalian genomes examined, including a monotreme species (platypus), whereas the LDHC gene may have arisen more recently in marsupial mammals.
authors.affiliation: School of Biomolecular and Physical Sciences, Griffith University, Nathan, Qld 4111, Australia. r.holmes@griffith.edu.au
authors.forename: Roger S
authors.initials: RS
authors.lastname: Holmes
authors.forename: Erwin
authors.initials: E
authors.lastname: Goldberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D003198:Computer Simulation; D030541:Databases, Genetic; D006801:Humans; D045022:Lactate Dehydrogenases / Q000235:genetics*; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009893:Opossums / Q000235:genetics; D010802:Phylogeny; D010987:Platypus / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D045022:Lactate Dehydrogenases
references.citation: Science. 1975 Jul 11;189(4197):102-14
references.pmid: 1138367
references.citation: Biol Reprod. 1989 Jan;40(1):173-80
references.pmid: 2923949
references.citation: Science. 1969 Apr 11;164(3876):185-7
references.pmid: 5774192
references.citation: Nucleic Acids Res. 2003 Jul 1;31(13):3497-500
references.pmid: 12824352
references.citation: Proteins. 2001 May 1;43(2):175-85
references.pmid: 11276087
references.citation: Biochem J. 1985 Nov 1;231(3):537-41
references.pmid: 3000353
references.citation: Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):1129-34
references.pmid: 9927705
references.citation: Biochem Biophys Res Commun. 1989 Mar 15;159(2):579-83
references.pmid: 2930531
references.citation: Biochem J. 1989 Feb 1;257(3):921-4
references.pmid: 2930497
references.citation: Nature. 2008 May 8;453(7192):175-83
references.pmid: 18464734
references.citation: FEBS Lett. 1972 Nov 15;28(1):51-5
references.pmid: 4646875
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Adv Enzymol Relat Areas Mol Biol. 1973;37:61-133
references.pmid: 4144036
references.citation: Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):242-6
references.pmid: 8419929
references.citation: Biol Reprod. 2008 Jul;79(1):26-34
references.pmid: 18367675
references.citation: Int J Mol Med. 2005 Jun;15(6):949-53
references.pmid: 15870898
references.citation: Mol Biol Evol. 1997 Nov;14(11):1081-7
references.pmid: 9364765
references.citation: Nat Genet. 2004 Jan;36(1):40-5
references.pmid: 14702039
references.citation: Electrophoresis. 1997 Dec;18(15):2714-23
references.pmid: 9504803
references.citation: J Exp Zool. 1998 Sep-Oct 1;282(1-2):280-3
references.pmid: 9723185
references.citation: Bioinformatics. 2000 Apr;16(4):404-5
references.pmid: 10869041
references.citation: J Exp Zool. 1967 Apr;164(3):309-16
references.pmid: 6068185
references.citation: Ann Hum Genet. 1989 Jul;53(3):215-9
references.pmid: 2596827
references.citation: Eur J Biochem. 1985 Feb 15;147(1):9-15
references.pmid: 3838278
references.citation: J Exp Zool. 1973 Dec;186(3):273-8
references.pmid: 4765351
references.citation: J Exp Zool. 1973 Apr;184(1):127-48
references.pmid: 4695811
references.citation: Genetics. 1987 May;116(1):99-105
references.pmid: 3036647
references.citation: Proc Natl Acad Sci U S A. 1987 Aug;84(15):5311-5
references.pmid: 2440048
references.citation: J Biol Chem. 1987 Sep 25;262(27):13155-62
references.pmid: 2443489
references.citation: Nucleic Acids Res. 2003 Jul 1;31(13):3381-5
references.pmid: 12824332
references.citation: Nature. 2007 May 10;447(7141):167-77
references.pmid: 17495919
references.citation: Genome Res. 2002 Jun;12(6):996-1006
references.pmid: 12045153
references.citation: Eur J Biochem. 1990 Apr 30;189(2):215-20
references.pmid: 2338075
references.citation: Nature. 2001 Feb 15;409(6822):860-921
references.pmid: 11237011
references.citation: Nucleic Acids Res. 2004 Jan 1;32(Database issue):D230-4
references.pmid: 14681401
references.citation: Nucleic Acids Res. 2007 Jan;35(Database issue):D610-7
references.pmid: 17148474
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Comp Biochem Physiol B. 1973 Dec;46(4):805-11
references.pmid: 4128505
references.citation: Eur J Biochem. 1996 Nov 1;241(3):779-86
references.pmid: 8944766
references.citation: Nature. 2002 Dec 5;420(6915):520-62
references.pmid: 12466850