Inhibitors of ABC transporters and biophysical methods to study their activity.
Multidrug resistance caused by the presence and overproduction of ABC transporters makes serious problems in cancer treating. The drugs administered during therapy are pumped outside the cell using the energy obtained from ATP hydrolysis. The augmented dosage of drugs to overcome the multidrug resistance is not sufficient. Thus knowledge of the structure of ABC proteins is necessary to understand the rules of their action. It could be also helpful to understand how the multidrug resistance could be overcome. One of the strategies involves the treatment of cancer cells with a mixture of anticancer drugs and inhibitors of ABC transporters. The yeast Saccharomyces cerevisiae, whose PDR pumps are analogues of mammalian MDR proteins responsible for multidrug resistance, is a suitable research model. Biophysical methods with different fluorescent dyes seem to be very suitable for the measurement of the efflux pump activity. This review describes some known inhibitors of ABC proteins and biophysical methods which could be used for measuring the ABC transporters activity.
Bartosiewicz
Dominika
D
Faculty of Biotechnology, Department of Lipids and Liposomes, Wroclaw University, Wroclaw, Poland.
Krasowska
Anna
A
D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D018432:Drug Resistance, Multiple; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D012235:Rhodamines / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; D000970:Antineoplastic Agents; D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D012235:Rhodamines; D000255:Adenosine Triphosphate