Prognostic implication of immunohistochemical Runx2 expression in osteosarcoma.
OBJECTIVE
Osteosarcoma is the most common primary bone malignancy. Many genetic markers have proven prognostic value in osteosarcoma and studies are under way to determine their potential application as specific therapeutic targets. Runx2, Indian hedgehog (IHH), and Sox9 are proteins that play major roles in bone formation and tumorigenesis. We studied the protein expression of Runx2, IHH, and Sox9 in osteosarcoma and correlated their expression with clinicopathological variables. We also studied the prognostic value of the expression of these three genes in osteosarcoma.


METHODS
We produced 48 formalin-fixed, paraffin-embedded tissue microarrays containing osteosarcoma tissue cores for immunohistochemical staining of Runx2, IHH and Sox9. We evaluated the expression of each gene by immunohistochemical staining and analyzed the relationship between expression and clinicopathological parameters.


RESULTS
High expression of Runx2 was significantly related to metastasis (P = 0.015). High expression of Runx2 indicated a trend toward a poor survival rate (P = 0.056). High expression of IHH and Sox9 were not related to any clinicopathological parameters.


CONCLUSIONS
High expression of Runx2 was significantly related to tumor metastasis in osteosarcoma. Our results suggest that overexpression of Runx2 might be a useful prognostic marker in osteosarcoma cases.
Won
Kyu Yeoun
KY
Department of Pathology, College of Medicine, Kyung Hee University, Seoul, Korea.
Park
Hye-Rim
HR
Park
Yong-Koo
YK
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001859:Bone Neoplasms / Q000737:chemistry* / Q000150:complications / Q000473:pathology* / Q000628:therapy; D002648:Child; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis*; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053823:Hedgehog Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000737:chemistry* / Q000150:complications / Q000473:pathology* / Q000628:therapy; D010146:Pain / Q000209:etiology; D010348:Patient Care Team; D011237:Predictive Value of Tests; D011379:Prognosis; D055758:SOX9 Transcription Factor / Q000032:analysis; D016019:Survival Analysis; D055815:Young Adult
D014408:Biomarkers, Tumor; D050659:Core Binding Factor Alpha 1 Subunit; D053823:Hedgehog Proteins; C509246:IHH protein, human; C493709:RUNX2 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human