Cytokine-based log-scale expansion of functional murine dendritic cells.
BACKGROUND
Limitations of the clinical efficacy of dendritic cell (DC)-based immunotherapy, as well as difficulties in their industrial production, are largely related to the limited number of autologous DCs from each patient. We here established a possible breakthrough, a simple and cytokine-based culture method to realize a log-scale order of functional murine DCs (>1,000-fold), which cells were used as a model before moving to human studies.


RESULTS
Floating cultivation of lineage-negative hematopoietic progenitors from bone marrow in an optimized cytokine cocktail (FLT3-L, IL-3, IL-6, and SCF) led to a stable log-scale proliferation of these cells, and a subsequent differentiation study using IL-4/GM-CSF revealed that 3-weeks of expansion was optimal to produce CD11b+/CD11c+ DC-like cells. The expanded DCs had typical features of conventional myeloid DCs in vitro and in vivo, including identical efficacy as tumor vaccines.


CONCLUSIONS
The concept of DC expansion should make a significant contribution to the progress of DC-based immunotherapy.
Harada
Yui
Y
Department of Gene Therapy, Chiba University Graduate School of Medicine, Chiba, Japan.
Ueda
Yasuji
Y
Kinoh
Hiroaki
H
Komaru
Atsushi
A
Fuji-Ogawa
Terumi
T
Furuya
Aki
A
Iida
Akihiro
A
Hasegawa
Mamoru
M
Ichikawa
Tomohiko
T
Yonemitsu
Yoshikazu
Y
D000818:Animals; D016207:Cytokines / Q000502:physiology*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL
D016207:Cytokines
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