Enterobacter arachidis sp. nov., a plant-growth-promoting diazotrophic bacterium isolated from rhizosphere soil of groundnut.
A methylotrophic nitrogen-fixing bacterial strain, Ah-143(T), isolated from the rhizosphere soil of field-grown groundnut was analysed by a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated strain Ah-143(T) to the family Enterobacteriaceae, with Enterobacter radicincitans and Enterobacter cowanii as the closest relatives. The strain is Gram-stain-negative, non-spore-forming, aerobic and motile, having straight rod-shaped cells with a DNA G+C content of approximately 53.2 mol%. The strain utilizes methanol as a carbon source and the mxaF gene was closely related to the mxaF gene of members of the genus Methylobacterium. The fatty acid profile consisted of C(16 : 0), C(17 : 0) cyclo, C(18 : 1)omega7c, summed feature 2 (iso-C(16 : 1) I and/or C(14 : 0) 3-OH) and summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c) as the major components. DNA-DNA relatedness of strain Ah-143(T) with its close relatives was less than 20 %. On the basis of the phylogenetic analyses, DNA-DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the strain represents a novel species of the genus Enterobacter and should be named Enterobacter arachidis sp. nov. The type strain is Ah-143(T) (=NCIMB 14469(T) =KCTC 22375(T)).
Madhaiyan
Munusamy
M
Biomaterials and Biocatalysts Group, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604.|Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
Poonguzhali
Selvaraj
S
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
Lee
Jung-Sook
JS
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yusong-gu, Daejeon 305-806, Republic of Korea.
Saravanan
Venkatakrishnan Sivaraj
VS
Department of Microbiology, Indira Gandhi College of Arts and Science, Pondicherry University, Kathirkamam, Pondicherry 605 009, India.
Lee
Keun-Chul
KC
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yusong-gu, Daejeon 305-806, Republic of Korea.
Santhanakrishnan
Palani
P
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
D010367:Arachis / Q000382:microbiology*; D001482:Base Composition; D001483:Base Sequence; D004754:Enterobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012988:Soil Microbiology
D005227:Fatty Acids