Comparison of assessment of oxygen consumption, Etest, and CLSI M38-A2 broth microdilution methods for evaluation of the susceptibility of Aspergillus fumigatus to posaconazole.
Posaconazole MICs for 50 Aspergillus fumigatus isolates with distinct genotypes were determined by three methods. MICs were > or = 0.5 microg/ml for 5, 11, and 15 strains by the CLSI reference M38-A2, Etest (48-h), and oxygen consumption methods, respectively. The levels of categorical agreement between the results obtained by the CLSI method and those obtained by the oxygen consumption and Etest methods were 80 and 84%, respectively.
Araujo
Ricardo
R
IPATIMUP, Institute of Pathology and Molecular Immunology, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. ricjparaujo@yahoo.com
Espinel-Ingroff
Ana
A
D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000378:metabolism; D025141:Drug Resistance, Fungal; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010101:Oxygen Consumption / Q000187:drug effects*; D014230:Triazoles / Q000494:pharmacology*
D000935:Antifungal Agents; D014230:Triazoles; C101425:posaconazole
Trends Microbiol. 2001 Aug;9(8):382-9
11514221
J Clin Microbiol. 2002 Jun;40(6):2101-7
12037072
Antimicrob Agents Chemother. 2008 Oct;52(10):3504-11
18644959
J Antimicrob Chemother. 1998 Oct;42(4):497-502
9818749
Antimicrob Agents Chemother. 2008 Jul;52(7):2468-72
18474574
Antimicrob Agents Chemother. 1996 May;40(5):1274-6
8723482
Antimicrob Agents Chemother. 2003 Feb;47(2):577-81
12543662
J Clin Microbiol. 2000 Sep;38(9):3457-9
10970403
Clin Microbiol Infect. 2009 Mar;15(3):260-6
19196262
PLoS Med. 2008 Nov 11;5(11):e219
18998768
J Clin Microbiol. 2008 Apr;46(4):1200-6
18234874
J Antimicrob Chemother. 2008 Dec;62(6):1277-80
18824456
Clin Ther. 2007 Sep;29(9):1862-86
18035188
Clin Microbiol Infect. 2006 Sep;12(9):901-4
16882296
Diagn Microbiol Infect Dis. 2003 Apr;45(4):241-4
12729993
J Antimicrob Chemother. 2007 Feb;59(2):230-7
17185296