title: Functional characterization of the in vitro folded human y(1) receptor in lipid environment.
abstract: We describe the recombinant production of the human Y(1) receptor from inclusion bodies of E. coli cultures. The in vitro refolding was carried out in the presence of lipids from bovine brain extracts. Y(1) receptors in brain lipids compete for cellular receptors in competitive binding experiments.
authors.affiliation: Institute of Biochemistry/Biotechnology, Martin Luther University Halle-Wittenberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schimmer
authors.forename: D
authors.initials: D
authors.lastname: Lindner
authors.forename: P
authors.initials: P
authors.lastname: Schmidt
authors.forename: A G
authors.initials: AG
authors.lastname: Beck-Sickinger
authors.forename: D
authors.initials: D
authors.lastname: Huster
authors.forename: R
authors.initials: R
authors.lastname: Rudolph
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001923:Brain Chemistry; D002417:Cattle; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism*; D009447:Neuroblastoma; D009478:Neuropeptide Y / Q000378:metabolism; D010759:Phosphorus Isotopes; D017510:Protein Folding; D017476:Receptors, Neuropeptide Y / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014316:Tritium
chemical_list: D008563:Membrane Lipids; D009478:Neuropeptide Y; D010759:Phosphorus Isotopes; D017476:Receptors, Neuropeptide Y; D011994:Recombinant Proteins; D014316:Tritium