title: [Isolation and characterization of collagen-binding domains from human von Willebrand factor].
abstract: DNA fragments encoding for two collagen binding decapeptides from the human von Willebrand factor (vWF-H1 and vWF-H2) were cloned in the Escherichia coil culture. Overproducing strains of the chimeric proteins vWF(H1)-CBD and vWF(H2)-CBD consisting of the corresponding decapeptide, Gly-Ser spacer and a cellulose binding domain (CBD) from Anaerocellum thermophilum were constructed. Using one-stage purification on cellulose, the highly purified samples of vWF(H1)-CBD and vWF(H2)-CBD proteins were obtained and the ability of these proteins to bind collagen was studied. These constructions are planned to be used for development of the recombinant collagen binding proteins with different biological activities, which, in its turn, will be used for development of the new generation products and materials for medicine, such as different kinds of implants, the coats, etc.
authors.forename: N E
authors.initials: NE
authors.lastname: Sharapova
authors.forename: A P
authors.initials: AP
authors.lastname: Kotnova
authors.forename: Z M
authors.initials: ZM
authors.lastname: Galushkina
authors.forename: N N
authors.initials: NN
authors.lastname: Poletaeva
authors.forename: N V
authors.initials: NV
authors.lastname: Lavrova
authors.forename: E I
authors.initials: EI
authors.lastname: Aksenova
authors.forename: A S
authors.initials: AS
authors.lastname: Semikhin
authors.forename: A S
authors.initials: AS
authors.lastname: Kariagina
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
mesh_terms: D003001:Cloning, Molecular; D003094:Collagen / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009842:Oligopeptides / Q000235:genetics*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014841:von Willebrand Factor / Q000737:chemistry* / Q000235:genetics
chemical_list: D009842:Oligopeptides; D011994:Recombinant Proteins; D014841:von Willebrand Factor; D003094:Collagen