title: Use of cultured human epithelium for coverage: a defect of radial forearm free flap donor site.
abstract: The radial forearm free flap has been popular in many areas of reconstructive surgery. Despite the many attributes of this flap in maxillofacial reconstruction, one of the disadvantages has been the morbidity of the donor site. Allogeneic cultured epidermis has been successfully applied on large second degree burns and on chronic leg ulcers. Autologous human keratinocytes and fibroblast equivalents can be cultured in-vitro from a small skin sample in order to produce a sufficient amount of epithelial autografts to cover the large defects of third-degree burn wounds. Interestingly, transplanted cultured epidermis retains characteristics of the original donor site. We report a case of a patient who underwent skin replacement by cultured epithelial autograft after wound breakdown occurred in the forearm donor site during the early postoperative period. This method could represent an auspicious alternative to conventional grafting methods for forearm free flap reconstruction. To the best of our knowledge, skin replacement by cultured epithelial autografts in this region has not been extensively described in the literature.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Central University Hospital, Oviedo, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Gallego
authors.forename: Luis
authors.initials: L
authors.lastname: Junquera
authors.forename: Pedro
authors.initials: P
authors.lastname: Villarreal
authors.forename: Ignacio
authors.initials: I
authors.lastname: Peña
authors.forename: Alvaro
authors.initials: A
authors.lastname: Meana
mesh_terms: D000368:Aged; D004848:Epithelium; D005260:Female; D005542:Forearm / Q000601:surgery*; D006801:Humans; D015603:Keratinocytes; D013524:Surgical Flaps; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods; D020858:Tissue and Organ Harvesting / Q000009:adverse effects*; D014945:Wound Healing