Pleiotropic effects of RecQ in Deinococcus radiodurans.
In Deinococcus radiodurans, there is a unique RecQ homolog (DR1289) with three-tandem HRDC domains. Deletion of drrecQ resulted in a low doubling rate and sensitivity to hydrogen peroxide. Here, we used cDNA microarray and biochemical assays to explore the physiological changes in the drrecQ mutant. The expressions of genes with predicted functions involved in iron homeostasis, antioxidant system, electron transport, and energy metabolism were significantly altered in response to drrecQ disruption. More reactive oxygen species (ROS) was accumulated in drrecQ mutant strain when compared to wild type. In addition, ICP-MS results showed that the intracellular level of iron was relatively higher, whereas the concentration of manganese was lower in drrecQ mutant than in wild type. Furthermore, our microarray data and pulsed-field gel results showed that DNA suffered more damage in drrecQ mutant than in wild type under 20 mM hydrogen peroxide stress. These results suggested that drrecQ is a gene of pleiotropic functions and contributes to the extraordinary resistance of D. radiodurans against stresses.
Chen
Huan
H
Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University 310029, China.
Huang
Lifen
L
Hua
Xiaoting
X
Yin
Longfei
L
Hu
Yihuai
Y
Wang
Chao
C
Chen
Weiwei
W
Yu
Xiaomin
X
Xu
Zhenjian
Z
Tian
Bing
B
Hu
Songnian
S
Hua
Yuejin
Y
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D034301:Deinococcus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D053484:RecQ Helicases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D008345:Manganese; D006861:Hydrogen Peroxide; D007501:Iron; D053484:RecQ Helicases