Constitutive expression of the ileS-2 gene responsible for high-level mupirocin resistance in Staphylococcus aureus.
Mupirocin is a topical antimicrobial agent that is used for the treatment of skin and postoperative wound infections, and the prevention of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA). However, the prevalence of mupirocin resistance in S. aureus, particularly in MRSA, has increased. High-level mupirocin resistance, with MICs >or=512 microg ml(-1), is mediated by the ileS-2 gene, which is located on conjugative plasmids. In the present study, we investigated whether mupirocin influences the expression of the ileS-2 gene responsible for high-level mupirocin resistance, and we present some evidence that this gene is not upregulated but constitutively expressed in S. aureus.
Oliveira
Naira Elane Moreira de
NEM
Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
Cavalcanti
Elisa D'Avila Costa
EDC
Instituto de Química, Bloco A, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
Laport
Marinella Silva
MS
Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
Bastos
Maria do Carmo de Freire
MDCF
Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
Giambiagi-deMarval
Marcia
M
Instituto de Microbiologia Prof. Paulo de Góes, Bloco I, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21491-590 Rio de Janeiro, RJ, Brazil.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D016712:Mupirocin / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D015854:Up-Regulation
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D016712:Mupirocin