title: Borrelia burgdorferi BmpA is a laminin-binding protein.
abstract: The Borrelia burgdorferi BmpA outer surface protein plays a significant role in mammalian infection by the Lyme disease spirochete and is an important antigen for the serodiagnosis of human infection. B. burgdorferi adheres to host extracellular matrix components, including laminin. The results of our studies indicate that BmpA and its three paralogous proteins, BmpB, BmpC, and BmpD, all bind to mammalian laminin. BmpA did not bind mammalian type I or type IV collagens or fibronectin. BmpA-directed antibodies significantly inhibited the adherence of live B. burgdorferi to laminin. The laminin-binding domain of BmpA was mapped to the carboxy-terminal 80 amino acids. Solubilized collagen inhibited BmpA-laminin binding, suggesting interactions through the collagen-binding domains of laminin. These results, together with previous data, indicate that BmpA and its paralogs are targets for the development of preventative and curative therapies for Lyme disease.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, MS 421 Chandler Medical Center, Lexington, KY 40536-0298, USA.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Verma
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Brissette
authors.forename: Amy
authors.initials: A
authors.lastname: Bowman
authors.forename: Brian
authors.initials: B
authors.lastname: Stevenson
mesh_terms: D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D003094:Collagen / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007797:Laminin / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C501736:BmpB protein, Borrelia burgdorferi; C505948:BmpD protein, Borrelia burgdorferi; D007797:Laminin; C088728:P39 antigen, Borrelia burgdorferi; D003094:Collagen
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