title: Electron microscopy of mammalian chromosomes.
abstract: This chapter describes methods of preparation of meiotic nuclei for their analysis with electron microscopy. Procedures include surface spreading as well as fixation, staining, and sectioning of tissue and subsequent 3-D reconstruction analysis. Immunostaining protocols for electron microscopy are provided. The rationale for using electron microscopy in place of light or fluorescent microscopy for specific cytological analyses is presented.
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada.
authors.forename: Peter
authors.initials: P
authors.lastname: Moens
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000737:chemistry / Q000648:ultrastructure*; D020732:Cytogenetic Analysis / Q000379:methods; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods