title: Improving mechanical and biological properties of macroporous HA scaffolds through composite coatings.
abstract: Interconnected porous hydroxyapatite (HA) scaffolds are widely used for bone repair and replacement, owing to their ability to support the adhesion, transfer, proliferation and differentiation of cells. In the present study, the polymer impregnation approach was adopted to produce porous HA scaffolds with three-dimensional (3D) porous structures. These scaffolds have an advantage of highly interconnected porosity (approximately 85%) but a drawback of poor mechanical strength. Therefore, the as-prepared HA scaffolds were lined with composite polymer coatings in order to improve the mechanical properties and retain its good bioactivity and biocompatibility at the same time. The composite coatings were based on poly(D,L-lactide) (PDLLA) polymer solutions, and contained single component or combination of HA, calcium sulfate (CS) and chondroitin sulfate (ChS) powders. The effects of composite coatings on scaffold porosity, microstructure, mechanical property, in vitro mineralizing behavior, and cell attachment of the resultant scaffolds were investigated. The results showed that the scaffolds with composite coatings resulted in significant improvement in both mechanical and biological properties while retaining the 3D interconnected porous structure. The in vitro mineralizing behaviors were mainly related to the compositions of CS and ChS powders in the composite coatings. Excellent cell attachments were observed on the pure HA scaffold as well as the three types of composite scaffolds. These composite scaffolds with improved mechanical properties and bioactivities are promising bone substitutes in tissue engineering fields.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: X
authors.initials: X
authors.lastname: Lu
authors.forename: K
authors.initials: K
authors.lastname: Duan
authors.forename: L Y
authors.initials: LY
authors.lastname: Guo
authors.forename: S B
authors.initials: SB
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Weng
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D019245:Compressive Strength / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D055595:Mechanical Phenomena / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D010316:Particle Size; D011140:Polyurethanes / Q000737:chemistry; D016062:Porosity / Q000187:drug effects; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D020099:Coated Materials, Biocompatible; D011140:Polyurethanes; D017886:Durapatite