title: Molecular cloning and characterization of four heat shock protein genes from Macrocentrus cingulum (Hymenoptera: Braconidae).
abstract: The full-length cDNA sequences of four HSPs genes were amplified from Macrocentrus cingulum, respectively named Mchsp90, Mchsc70, Mchsp70 and Mchsp23.8. These four genes were submitted to GenBank database and assigned to GenBank accession number: EU570065, EU585780, EU585779 and EU624206. Phylogenetic analysis indicated that the Mchsp90, Mchsc70 and Mchsp70 were located in cytoplasm. Moreover, the Mchsc70 was a member of heat shock cognate protein 70 while the Mchsp70 was a member of heat shock inducible 70. The expression analysis indicated that these four genes were differentially expressed at larval, pupal and adult stages. Under normal conditions, the mRNA levels of Mchsp90 and Mchsc70 were higher than Mchsp70 while Mchsp23.8 was decreased during aging. The mRNA levels of these four genes could be up-regulated by heat shock besides Mchsp70 and Mchsp23.8 could be increased more dramatically than Mchsp90 and Mchsc70. These results suggested that Mchsp90, Mchsp70 and Mchsp23.8 might have dual functions under normal and heat shock conditions while Mchsp70 and Mchsp23.8 might supply more important protection under heat shock.
authors.affiliation: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, 100101, Datunlu, Chaoyang, Beijing, People's Republic of China. xupj@ioz.ac.cn
authors.forename: Pengjun
authors.initials: P
authors.lastname: Xu
authors.forename: Jinhua
authors.initials: J
authors.lastname: Xiao
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.forename: Dawei
authors.initials: D
authors.lastname: Huang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics; D006927:Hymenoptera / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C405528:heat-shock protein 23.8
references.citation: Mol Biol Evol. 1995 Nov;12(6):1063-73
references.pmid: 8524040
references.citation: Mol Biol Evol. 2004 Mar;21(3):498-505
references.pmid: 14694072
references.citation: Cell Stress Chaperones. 2007 Autumn;12(3):199-204
references.pmid: 17915551
references.citation: Insect Mol Biol. 2007 Apr;16(2):207-19
references.pmid: 17298555
references.citation: J Cell Biol. 2001 Jul 23;154(2):267-73
references.pmid: 11470816
references.citation: FEBS Lett. 2007 Jul 31;581(19):3702-10
references.pmid: 17544402
references.citation: Mol Cell Biol. 1998 Apr;18(4):2023-8
references.pmid: 9528774
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Gene. 2001 Jun 27;271(2):223-31
references.pmid: 11418243
references.citation: Gene Expr Patterns. 2006 Jan;6(2):127-33
references.pmid: 16326146
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2005 May;141(1):60-9
references.pmid: 15890552
references.citation: J Insect Physiol. 2007 Apr;53(4):385-91
references.pmid: 17349654
references.citation: Cell Stress Chaperones. 2007 Summer;12(2):172-85
references.pmid: 17688196
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2005 Jun;141(2):247-56
references.pmid: 15979913
references.citation: Pharmacol Ther. 1998 Nov;80(2):183-201
references.pmid: 9839771
references.citation: Nature. 1998 Nov 26;396(6709):336-42
references.pmid: 9845070
references.citation: Genomics. 2005 Dec;86(6):627-37
references.pmid: 16269234
references.citation: Insect Biochem Mol Biol. 2000 Jun;30(6):515-21
references.pmid: 10802243
references.citation: Dev Genes Evol. 2007 Aug;217(8):603-12
references.pmid: 17647016
references.citation: J Biol Chem. 2000 Jan 14;275(2):1095-104
references.pmid: 10625651
references.citation: Brief Bioinform. 2004 Jun;5(2):150-63
references.pmid: 15260895
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2007 May;147(1):112-21
references.pmid: 17267255
references.citation: J Mol Evol. 1998 Nov;47(5):565-77
references.pmid: 9797407
references.citation: J Insect Physiol. 2003 Apr;49(4):367-75
references.pmid: 12769990
references.citation: Theriogenology. 2007 Sep 1;68(4):626-32
references.pmid: 17590427
references.citation: Mech Dev. 2008 Nov-Dec;125(11-12):1048-58
references.pmid: 18718532
references.citation: Mol Biol (Mosk). 2008 Mar-Apr;42(2):265-74
references.pmid: 18610835
references.citation: Cell Stress Chaperones. 2003 Summer;8(2):144-52
references.pmid: 14627200
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11130-7
references.pmid: 17522254
references.citation: FEBS J. 2007 Sep;274(17):4453-63
references.pmid: 17681020
references.citation: Mol Cell Biol. 1989 Jun;9(6):2615-26
references.pmid: 2527334
references.citation: Insect Mol Biol. 1996 Feb;5(1):73-9
references.pmid: 8630537
references.citation: Cell Stress Chaperones. 2004 Winter;9(4):332-43
references.pmid: 15633291
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: Insect Mol Biol. 2006 Dec;15(6):839-52
references.pmid: 17201776
references.citation: Arch Insect Biochem Physiol. 2006 Jun;62(2):80-90
references.pmid: 16703614
references.citation: J Mol Evol. 1994 Jan;38(1):1-17
references.pmid: 8151709