Endoscopic approaches to manage in vitro and in vivo embryo development: use of the bovine oviduct.
The oviduct plays a major part in different reproductive processes providing the microenvironment for numerous steps in early embryogenesis. Consequently, there is a growing demand to perform comparative studies focusing on causal mechanisms related to embryo development within its environment including complex and holistic strategies. However, the routine flushing and transfer procedure of bovine embryos is limited to the morula and blastocyst stage. Additionally, the use of in vitro production of bovine embryos provides access to an extra amount of embryos at various stages. But the quality of these embryos does not reflect the quality of its ex vivo counterparts. For two decades our own studies have focused on use of the oviductal environment of different species to optimize early embryo development for different purposes. The current article briefly highlights some main characteristics of the fallopian tube and reviews the endoscopic approach to access the fallopian tube using the stepwise minimal invasive technique established in different species.
Besenfelder
U
U
Reproduction Center Wieselburg, University of Veterinary Medicine, Vienna, Austria. urban.besenfelder@boku.ac.at
Havlicek
V
V
Kuzmany
A
A
Brem
G
G
D000818:Animals; D001947:Breeding / Q000379:methods; D002417:Cattle; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary*; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary; D047108:Embryonic Development; D004724:Endoscopy / Q000379:methods / Q000662:veterinary*; D005187:Fallopian Tubes; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D011817:Rabbits; D012418:Ruminants; D013552:Swine