title: Effect of the polymorphism in 5' UTR region of pig prolactin gene on prolactin gene expression and reproduction performance in the female pig.
abstract: OBJECTIVE
  The recent genetics and molecular biology progress seems to be a fascinating challenge for the interdisciplinary studies on the effects of genetic changes in gene structure that causes the modification of physiological functions of many important proteins including hormones. Pig prolactin is one of the interesting hormones for this study.
  
  
  OBJECTIVE
  The aim of the study was to analyze the mutation in 5'UTR region of the pig prolactin (PRL) gene and to evaluate the effect of this polymorphism on changes in plasma prolactin concentration.
  
  
  RESULTS
  It was found that only two individual groups of animals differed by the genotype in examined PRL gene locus - homozygote C/C and heterozygote C/T. PRL plasma concentration was 38.4 ng/ml (for C/T animals) or 42.7 ng/ml (for C/C animals). Animals with C/C genotyped exhibited a tendency to elevate PRL concentration as compared to the C/T group (p< 0.07).
  
  
  CONCLUSIONS
  This research combines the genetic, molecular and, in vivo, physiological study which allows focus on the possible relationship between the gene polymorphism and physiological status of animal.
authors.affiliation: Institute of Genetics and Animal Breeding, Polish Academy of Sciences in Jastrzebiec, Warsaw, Poland. a.kossakowska@ighz.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Korwin-Kossakowska
authors.forename: Joanna
authors.initials: J
authors.lastname: Wyszynska-Koko
authors.forename: Grazyna
authors.initials: G
authors.lastname: Sender
authors.forename: Alina
authors.initials: A
authors.lastname: Gajewska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Pierzchala
authors.forename: Marian
authors.initials: M
authors.lastname: Kamyczek
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Kochman
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D005260:Female; D015870:Gene Expression; D005838:Genotype; D009154:Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011388:Prolactin / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D012098:Reproduction / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics / Q000502:physiology*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism; D013997:Time Factors
chemical_list: D020121:5' Untranslated Regions; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin