Little heterogeneity among genes encoding heat-labile and heat-stable toxins of enterotoxigenic Escherichia coli strains isolated from diarrheal pigs.
To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene has only two mutations in 4 (of 52) strains, and that both mutations cause a reduction in GM1 binding and toxicity.
Zhang
Chengxian
C
Department of Veterinary Science, South Dakota State University, The Center for Infectious Disease Research & Vaccinology, Brookings, South Dakota, USA.
Rausch
Dana
D
Zhang
Weiping
W
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004768:Enterotoxins / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics*; D020125:Mutation, Missense; D011110:Polymorphism, Genetic; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli
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