title: Immune responses of human immature dendritic cells can be modulated by the recombinant Aspergillus fumigatus antigen Aspf1.
abstract: Invasive aspergillosis is a significant cause of morbidity and mortality in patients after stem cell transplantation, in solid organ transplant recipients, and in patients with hematological malignancies. The interactions between human immature dendritic cells (iDCs) and Aspergillus fumigatus antigens are widely uncharacterized. We analyzed the immune response of iDCs to different recombinant A. fumigatus antigens (Aspf1 and Crf1). One of these antigens, the 18-kDa RNase Aspf1, triggered the increased level of expression of genes encoding proinflammatory cytokines and chemokines, and augmented the activation of NFkappaB and the apoptosis of iDCs. Furthermore, by fluorescence microscopy, we could demonstrate that in the first 3 h a major portion of Aspf1 accumulates on the cell surface. Finally, we could show an increased segregation of cytokines and chemokines after the stimulation of iDCs by an Aspf1 deletion mutant strain of A. fumigatus.
authors.affiliation: Universität Würzburg, Medizinische Klinik & Poliklinik II, Institut für Hygiene & Mikrobiologie, Würzburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Ok
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Latgé
authors.forename: Carina
authors.initials: C
authors.lastname: Baeuerlein
authors.forename: Frank
authors.initials: F
authors.lastname: Ebel
authors.forename: Markus
authors.initials: M
authors.lastname: Mezger
authors.forename: Max
authors.initials: M
authors.lastname: Topp
authors.forename: Oliver
authors.initials: O
authors.lastname: Kurzai
authors.forename: Doreen
authors.initials: D
authors.lastname: Killian
authors.forename: Markus
authors.initials: M
authors.lastname: Kapp
authors.forename: Goetz-Ulrich
authors.initials: GU
authors.lastname: Grigoleit
authors.forename: Helga
authors.initials: H
authors.lastname: Sennefelder
authors.forename: Javier
authors.initials: J
authors.lastname: Arroyo
authors.forename: Hermann
authors.initials: H
authors.lastname: Einsele
authors.forename: Juergen
authors.initials: J
authors.lastname: Loeffler
mesh_terms: D000485:Allergens / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017951:Antigen Presentation; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000235:genetics; D052179:Antigens, Plant; D017209:Apoptosis; D001228:Aspergillosis / Q000209:etiology / Q000276:immunology / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D001483:Base Sequence; D002454:Cell Differentiation; D018920:Coculture Techniques; D004271:DNA, Fungal / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005656:Fungal Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005800:Genes, Fungal; D019337:Hematologic Neoplasms / Q000150:complications; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D009154:Mutation; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D019737:Transplants / Q000009:adverse effects
chemical_list: C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D000946:Antigens, Fungal; D052179:Antigens, Plant; D004271:DNA, Fungal; D005656:Fungal Proteins; D011994:Recombinant Proteins
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