Hyaluronic acid and HYAL-1 in prostate biopsy specimens: predictors of biochemical recurrence.
OBJECTIVE
Molecular markers could aid prostate specific antigen, biopsy Gleason sum and clinical stage to provide accurate information on prostate cancer progression. HYAL-1 hyaluronidase and hyaluronic acid staining in prostatectomy specimens predicts biochemical recurrence. We examined whether hyaluronic acid and HYAL-1 staining in biopsy specimens predicts biochemical recurrence and correlates with staining in matched prostatectomy specimens.


METHODS
Biopsy and prostatectomy specimens were obtained from 61 patients with clinically localized prostate cancer from multiple centers, including 23 with (group 1) and 38 without (group 2) biochemical recurrence. Mean followup was 103.1 months. Biotinylated hyaluronic acid binding protein and anti-HYAL-1 antibody were used for hyaluronic acid and HYAL-1 staining, respectively. Staining was graded between 0 and 300 depending on staining intensity and area.


RESULTS
HYAL-1 and hyaluronic acid were expressed in tumor cells and stroma, respectively. In biopsy specimens HYAL-1 and hyaluronic acid expression was higher in group 1 than in group 2 (203.9 and 182.1 vs 48.8 and 87.0, respectively, p <0.0001). On univariate analysis hyaluronic acid, HYAL-1, biopsy Gleason and prostate specific antigen significantly predicted biochemical recurrence (p <0.001). On multivariate analysis only HYAL-1 staining independently predicted recurrence with an accuracy of 81.8% (p <0.001). In prostatectomy specimens only HYAL-1 staining correlated with staining in biopsy specimens (Spearman rho = 0.72, p = 0.0002) and predicted biochemical recurrence.


CONCLUSIONS
To our knowledge this is the first report that HYAL-1 staining in biopsy specimens is an independent predictor of biochemical recurrence. This may be useful when selecting treatment.
Gomez
Christopher S
CS
Department of Urology, University of Miami Miller School of Medicine, Miami, Florida 33101, USA.
Gomez
Pablo
P
Knapp
Judith
J
Jorda
Merce
M
Soloway
Mark S
MS
Lokeshwar
Vinata B
VB
D000368:Aged; D001706:Biopsy; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis*; D006821:Hyaluronoglucosaminidase / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000737:chemistry* / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011467:Prostate / Q000737:chemistry* / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000175:diagnosis*
D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase
Cancer Res. 1999 Sep 1;59(17):4464-70
10485499
Clin Cancer Res. 2008 Jul 1;14(13):4111-8
18593988
Am J Pathol. 2009 Mar;174(3):1027-36
19218337
Cancer Res. 2003 May 15;63(10):2638-44
12750291
J Urol. 2004 Sep;172(3):1123-6
15311054
Urol Oncol. 2007 Sep-Oct;25(5):413-9
17826663
J Biol Chem. 2007 Jul 13;282(28):20561-72
17502371
Cancer Res. 2005 Mar 15;65(6):2243-50
15781637
Eur J Cancer. 2001 May;37(7):849-56
11313172
Urol Int. 2002;69(4):266-72
12444281
J Urol. 2000 Jan;163(1):348-56
10604388
J Urol. 1999 Dec;162(6):2040-5
10569564
Eur Urol. 2008 Jan;53(1):68-80
17920184
Semin Cancer Biol. 2008 Aug;18(4):281-7
18448355
Eur Urol. 2010 Jan;57(1):86-93
19345473
Int J Cancer. 2004 Oct 20;112(1):121-9
15305383
Cancer Res. 2005 Sep 1;65(17):7782-9
16140946
Adv Anat Pathol. 2008 Nov;15(6):319-31
18948763
Cancer Res. 1996 Feb 1;56(3):651-7
8564986
Carcinogenesis. 2008 Feb;29(2):282-90
18174258
Cancer Res. 2008 Jan 15;68(2):483-91
18199543
Front Biosci. 2008 May 01;13:5664-80
18508614
Semin Cancer Biol. 2008 Aug;18(4):288-95
18468453