vanA in Enterococcus faecium, Enterococcus faecalis, and Enterococcus casseliflavus detected in French cattle.
The goal of this study was to assess the presence of enterococci species presenting van-mediated glycopeptide resistance in French cattle. Fecal samples were collected from healthy and sick animals, and enterococci were screened for vancomycin resistance. Vancomycin resistance was principally encountered in Enterococcus gallinarum and Enterococcus casseliflavus strains. However, glycopeptide resistance was detected in three different species of enterococci (E. faecalis, E. faecium, and E. casseliflavus). Molecular characterization of the genetic support proved that they all presented the prototypic VanA element. Interestingly, the E. casseliflavus strain displayed a remarkable VanB phenotype/vanA-vanC genotype. Transferability, associated resistances, and factors of vanA cotransfer were sought. This study proved that acquired vanA genes can still be detected in food-producing animals more than a decade after the avoparcin ban. Indeed, calves, which are recurrently exposed to antibiotics in France, may allow the re-emergence of glycopeptide resistance through coselection factors, and this might potentially be concerning for human health.
Haenni
Marisa
M
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité Bactériologie Bovine et Hygiène des Viandes, Lyon, France.
Saras
Estelle
E
Châtre
Pierre
P
Meunier
Danièle
D
Martin
Sylvie
S
Lepage
Gérard
G
Ménard
Marie-Françoise
MF
Lebreton
Patricia
P
Rambaud
Thomas
T
Madec
Jean-Yves
JY
D000822:Animal Husbandry / Q000331:legislation & jurisprudence / Q000379:methods; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D019729:Carbon-Oxygen Ligases / Q000235:genetics* / Q000378:metabolism; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D013293:Enterococcus faecalis / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005517:Foodborne Diseases / Q000517:prevention & control; D005602:France / Q000453:epidemiology; D022761:Gene Transfer, Horizontal; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008826:Microbial Sensitivity Tests; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D020713:Vancomycin Resistance / Q000235:genetics*
D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus