title: Immunoproteomic analysis of outer membrane proteins and extracellular proteins of Actinobacillus pleuropneumoniae JL03 serotype 3.
abstract: BACKGROUND
  Actinobacillus pleuropneumoniae is the causative agent of porcine contagious pleuropneumonia, a highly contagious respiratory infection in pigs, and all the 15 serotypes are able to cause disease. Current vaccines including subunit vaccines could not provide satisfactory protection against A. pleuropneumoniae. In this study, the immunoproteomic approach was applied to the analysis of extracellular and outer membrane proteins of A. pleuropneumoniae JL03 serotype 3 for the identification of novel immunogenic proteins for A. pleuropneumoniae.
  
  
  RESULTS
  A total of 30 immunogenic proteins were identified from outer membrane and extracellular proteins of JL03 serotype 3, of which 6 were known antigens and 24 were novel immunogenic proteins for A. pleuropneumoniae.
  
  
  CONCLUSIONS
  These data provide information about novel immunogenic proteins for A. pleuropneumoniae serotype 3, and are expected to aid in development of novel vaccines against A. pleuropneumoniae.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Hubei, PR China. ebrain04@163.com
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Liao
authors.forename: Junhua
authors.initials: J
authors.lastname: Deng
authors.forename: Anding
authors.initials: A
authors.lastname: Zhang
authors.forename: Mingguang
authors.initials: M
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D020543:Proteome / Q000276:immunology / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D020543:Proteome
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