Detection of Plasmodium knowlesi by real-time polymerase chain reaction.
We previously developed a real-time polymerase chain reaction (PCR) assay for detection of the four Plasmodium species that infect humans. Recent studies have shown that natural transmission of the simian parasite Plasmodium knowlesi to humans occurs frequently in Southeast Asia. We have expanded our PCR assay to include detection of P. knowlesi.
Babady
N Esther
NE
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, USA.
Sloan
Lynne M
LM
Rosenblatt
Jon E
JE
Pritt
Bobbi S
BS
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000145:classification / Q000302:isolation & purification; D004854:Herpesvirus 4, Human; D054884:Host-Pathogen Interactions; D016790:Plasmodium knowlesi / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity; D014122:Toxoplasma; D054851:Tropheryma
D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S