Studies on the production of actinomycin-D by Streptomyces griseoruber--a novel source.
OBJECTIVE
To isolate and characterize bioactive metabolites produced by a micro-organism isolated from a soil sample associated with the roots of a medicinal plant, Azadirachta indica.


RESULTS
Morphological, cultural, physiological and 16S rRNA homology studies revealed that the organism showed 99% similarity with Streptomyces griseoruber NBRC 12873. One bioactive metabolite (Py2) isolated from the fermented broth was characterized as actinomycin-D (act-D). It showed high activity against various gram-positive and gram-negative bacterial cultures, Mycobacterium tuberculosis H37Rv and human neoplastic cells in vitro using standard protocols.


CONCLUSIONS
The isolated strain S. griseoruber produced act-D predominantly (210 mg l(-1), c. 88% of the crude) under nonoptimized growth conditions.


CONCLUSIONS
Streptomyces griseoruber may be exploited as a potential source for the commercial production of act-D, as this strain is not reported to produce act-D. Further investigations on the strain for commercial application will be of immense pharmaceutical importance.
Praveen
V
V
Division of Fermentation Technology, Central Drug Research Institute, Lucknow, India.
Tripathi
C K M
CK
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology; D031601:Azadirachta / Q000382:microbiology*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D003609:Dactinomycin / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D003609:Dactinomycin