Probing key targets in insulin signaling and adipogenesis using a methanolic extract of Costus pictus and its bioactive molecule, methyl tetracosanoate.
A methanolic extract of Costus pictus (CPME) showed optimum anti-diabetic activity at 100 ng/ml. Bioactivity-guided purification of CPME led to the isolation of methyl tetracosanoate (MT) which showed an optimum glucose uptake at 1 ng/ml. CPME at 10 mug/ml inhibited adipogenesis whereas fully differentiated adipocytes exhibited a 3-fold increase in lipid accumulation compared to pre-adipocytes. Gene and protein expression of key targets in insulin signaling and adipogenesis pathway revealed that CPME exhibited anti-diabetic activity along with anti-adipogenic activity whereas MT demonstrated only anti-diabetic activity.
Shilpa
Kusampudi
K
Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, 600025, India.
Sangeetha
Kadapakkam Nandabalan
KN
Muthusamy
Velusamy Shanmuganathan
VS
Sujatha
Sundaresan
S
Lakshmi
Baddireddi Subadhra
BS
D017667:Adipocytes / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002460:Cell Line; D029770:Costus / Q000737:chemistry*; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
D005227:Fatty Acids; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C048681:methyl tetracosanoic acid