Effects of cigarette smoking on mucin production in human middle ear epithelial cells.
OBJECTIVE
Otitis media (OM) is the most common disease in preschool age children related to passive cigarette smoking as risk factor. In this study, we investigate whether the cigarette smoking can induce the inflammation in human middle ear epithelial cell, and cigarette smoke-induced inflammation can increase the expression of MUC5AC gene and protein that was known to play an important role in OM with effusion.


METHODS
After treatment of cigarette smoke solution (CSS) on immortalized human middle ear epithelial cells (HMEECs) with or without pretreatment by epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (AG1478), we observed expression of tumor necrosis factor-alpha (TNF-α), EGFR, MUC5AC mRNA by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and EGFR, MUC5AC protein by western blotting.


RESULTS
Treatment of CSS increased expression of TNF-α mRNA dose dependently. Treatment of CSS upregulated the EGFR and MUC5AC mRNA in a time-dependent manner. CSS-induced upregulation of EGFR and MUC5AC mRNA was suppressed by the pretreatment of AG1478. EGFR and MUC5AC proteins were upregulated by the treatment of CSS and suppressed by the pretreatment of AG1478.


CONCLUSIONS
Treatment of CSS on HMEECs increased the expression of MUC5AC mRNAs and proteins which play a major role in OM with effusion.
Cho
Jae-Gu
JG
Department of Otolaryngology-Head and Neck Surgery, Guro Hospital, Korea University College of Medicine, 80 Guro-dong, Guro-gu, Seoul 152-703, South Korea.
Woo
Jeong-Soo
JS
Lee
Heung-Man
HM
Jung
Hak Hyun
HH
Hwang
Soon-Jae
SJ
Chae
Sungwon
S
D000704:Analysis of Variance; D015153:Blotting, Western; D002478:Cells, Cultured; D004432:Ear, Middle / Q000166:cytology; D004847:Epithelial Cells / Q000378:metabolism*; D066246:ErbB Receptors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009077:Mucins / Q000096:biosynthesis*; D010033:Otitis Media / Q000235:genetics / Q000503:physiopathology; D011799:Quinazolines; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D020545:Respiratory Mucosa / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology*; D015854:Up-Regulation
D009077:Mucins; D011799:Quinazolines; D012333:RNA, Messenger; D012906:Smoke; D014409:Tumor Necrosis Factor-alpha; D020032:Tyrphostins; C101044:RTKI cpd; D066246:ErbB Receptors