New approach for m-cell-specific molecules screening by comprehensive transcriptome analysis.
A minor population of M cells within the follicle-associated epithelium (FAE) of intestinal Peyer's patches (PPs) serves as a major portal for entry of exogenous antigens. Characterization of the mammalian M cells, including identification of M-cell surface molecules used for bacterial uptake, has been hampered by their relative rarity. In contrast, M cells constitute virtually all of the FAE cells in the avian bursa of Fabricius. We therefore performed comparative gene expression profiling of chicken and murine FAE to identify commonly expressed genes by M cells in both species. The comprehensive transcriptome analysis revealed that 28 genes were commonly up-regulated in FAE from both species. In situ hybridization revealed that annexin A10 (Anxa10) mRNA was scattered in FAE, and co-localized with Ulex europaeus agglutinin-1 binding to M cells. Whole-mount immunostaining also revealed that cellular prion protein (PrP(C)) was expressed on the luminal side of the apical plasma membrane of M cells, and co-localized with grycoprotein 2 that recognizes only M cells in murine PP. Our findings identify new M-cell-specific molecules through using comprehensive transcriptome analysis. These conserved molecules in M cells of mice and chickens may play essential roles in M-cell function and/or differentiation.
Nakato
Gaku
G
International Graduate School of Arts and Sciences, Yokohama City University, Kanagawa 230-0045, Japan.
Fukuda
Shinji
S
Hase
Koji
K
Goitsuka
Ryo
R
Cooper
Max D
MD
Ohno
Hiroshi
H
D000818:Animals; D017302:Annexins / Q000378:metabolism*; D002060:Bursa of Fabricius / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D010581:Peyer's Patches / Q000166:cytology* / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism; D018621:PrPC Proteins / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D015854:Up-Regulation
D017302:Annexins; C485665:Anxa10 protein, mouse; D006023:Glycoproteins; D037121:Plant Lectins; D018621:PrPC Proteins; D011506:Proteins; C036850:Ulex europaeus lectins
Biochem Biophys Res Commun. 2007 Mar 23;354(4):949-54
17276393
J Biol Chem. 2004 Dec 10;279(50):52623-9
15381697
Bioinformatics. 2007 Nov 1;23(21):2934-41
17893089
J Pathol. 2006 Jun;209(2):240-9
16552796
Clin Exp Immunol. 2006 Oct;146(1):1-8
16968391
J Exp Med. 1994 Jul 1;180(1):15-23
8006579
FEMS Microbiol Lett. 2001 Apr 13;197(2):131-8
11313125
Immunol Today. 1990 Apr;11(4):129-36
2187470
J Immunol. 2008 Jun 15;180(12):7840-6
18523247
J Biol Chem. 2007 Mar 9;282(10):7465-71
17218310
J Histochem Cytochem. 1993 Nov;41(11):1679-87
7691933
Bioinformatics. 2004 Jun 12;20(9):1464-5
14962934
Gut. 1990 Sep;31(9):1011-5
2210445
Nat Immunol. 2001 Nov;2(11):1004-9
11685223
Histochem Cell Biol. 2008 Mar;129(3):311-20
18097679
Nature. 1997 Oct 9;389(6651):636-9
9335508
J Endocrinol. 2007 Dec;195(3):385-92
18000301
Genome Biol. 2004;5(4):219
15059252
Gastroenterology. 1977 Mar;72(3):440-51
832793
J Pathol. 2006 Aug;209(4):484-91
16767691
Nat Rev Mol Cell Biol. 2005 Jun;6(6):449-61
15928709
J Exp Med. 2003 Jul 7;198(1):5-17
12847134
Infect Immun. 1998 Mar;66(3):1237-43
9488419
EMBO J. 1999 Jun 15;18(12):3193-203
10369660
Exp Cell Res. 2001 Sep 10;269(1):13-22
11525635
DNA Res. 2005;12(2):127-37
16303744
Clin Exp Immunol. 2006 Jul;145(1):1-4
16792666
Am J Pathol. 2002 May;160(5):1831-7
12000734
Science. 1982 Apr 9;216(4542):136-44
6801762
Am J Anat. 1973 Apr;136(4):455-77
4692973