Cell-free Escherichia coli-based system to screen for quorum-sensing molecules interacting with quorum receptor proteins of Streptomyces coelicolor.
Quorum sensing (QS) is mediated by small molecules and involved in diverse cellular functions, such as virulence, biofilm formation, secondary metabolism, and cell differentiation. In this study, we developed a rapid and effective screening tool based on a cell-free Escherichia coli-based expression system to identify QS molecules of Streptomyces. The binding of QS molecules to gamma-butyrolactone receptor ScbR was monitored by changes in the expression levels of the green fluorescent protein reporter in E. coli cell extract. Using this assay system, we could successfully confirm SCB1, a gamma-butyrolactone molecule in Streptomyces coelicolor, binding to its known receptor, ScbR. In addition, we have shown that N-hexanoyl-DL-homoserine lactone, one of the QS molecules in many gram-negative bacteria, can regulate ScbR and trigger precocious antibiotic production in S. coelicolor. Our new method can be applied to other strains for which a screening tool for QS molecules has not yet been developed.
Yang
Yung-Hun
YH
School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea. byungkim@snu.ac.kr.
Kim
Tae-Wan
TW
Park
Sung-Hee
SH
Lee
Kwangwon
K
Park
Hyung-Yeon
HY
Song
Eunjung
E
Joo
Hwang-Soo
HS
Kim
Yun-Gon
YG
Hahn
Ji-Sook
JS
Kim
Byung-Gee
BG
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015374:Biosensing Techniques; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D011485:Protein Binding; D053038:Quorum Sensing; D048372:Streptomyces coelicolor / Q000378:metabolism / Q000502:physiology*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C446304:ScbR protein, Streptomyces coelicolor; C088386:homoserine lactone; D049452:Green Fluorescent Proteins; D015107:4-Butyrolactone
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