title: Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population.
abstract: Since the discovery of human bocavirus (hBoV), the virus has been detected worldwide in respiratory tract samples from young children by various polymerase chain reaction (PCR) assays and real-time PCRs (Q-PCR). Until now, no data have been reported on the presence of hBoV in Belgium and the detection of hBoV in a multiplex Q-PCR setting has not been described. The aim of this study was to develop a fast and reliable multiplex Q-PCR for the simultaneous detection of hBoV DNA and adenovirus (AdV) DNA. During the winter of 2004-2005, 445 nasopharyngeal aspirates (NPAs) were analysed from 404 Belgian children up to 5 years old with acute respiratory tract infections (ARTIs). (Co)infections with hBoV, AdV, respiratory syncytial virus (RSV), human metapneumovirus (hMPV) and influenza A virus were investigated. A viral agent was detected in 61% (n = 272/445) of the NPAs. Multiplex Q-PCR found a prevalence of 11% (n = 51/445) hBoV and 13% (n = 58/445) AdV. Coinfections were more frequently found with AdV (62%; n = 36/58) than with hBoV (49%; n = 25/51). Follow-up samples were available from 22 patients with ARTIs. In three patients, hBoV DNA persisted for one month. Multiplex Q-PCR may help in closing the diagnostic gap by addressing a broader range of potential respiratory pathogens.
authors.affiliation: Laboratory of Clinical Biology, Department of Microbiology and Molecular Biology, Onze Lieve Vrouw (OLV) Hospital, Moorselbaan 164, 9300, Aalst, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: De Vos
authors.forename: A
authors.initials: A
authors.lastname: Vankeerberghen
authors.forename: F
authors.initials: F
authors.lastname: Vaeyens
authors.forename: K
authors.initials: K
authors.lastname: Van Vaerenbergh
authors.forename: A
authors.initials: A
authors.lastname: Boel
authors.forename: H
authors.initials: H
authors.lastname: De Beenhouwer
mesh_terms: D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology*; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification*; D000454:Aleutian Mink Disease Virus / Q000302:isolation & purification; D001530:Belgium / Q000453:epidemiology; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods; D015897:Comorbidity; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D056661:Human bocavirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000302:isolation & purification; D008297:Male; D029121:Metapneumovirus / Q000302:isolation & purification; D009305:Nasopharynx / Q000821:virology; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000821:virology; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004279:DNA, Viral
references.citation: J Infect Dis. 2007 Apr 1;195(7):1038-45
references.pmid: 17330795
references.citation: Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12891-6
references.pmid: 16118271
references.citation: J Clin Virol. 2007 Nov;40 Suppl 1:S24-30
references.pmid: 18162251
references.citation: Clin Infect Dis. 2007 Apr 1;44(7):904-10
references.pmid: 17342639
references.citation: J Clin Microbiol. 2007 Jul;45(7):2116-22
references.pmid: 17475762
references.citation: Curr Opin Pediatr. 2008 Feb;20(1):62-6
references.pmid: 18197041
references.citation: J Clin Virol. 2007 Sep;40(1):9-14
references.pmid: 17714984
references.citation: Clin Infect Dis. 2006 Aug 1;43(3):283-8
references.pmid: 16804840
references.citation: J Clin Microbiol. 2006 Mar;44(3):1132-4
references.pmid: 16517912
references.citation: J Clin Virol. 2007 Nov;40(3):229-35
references.pmid: 17851126
references.citation: Emerg Infect Dis. 2007 Apr;13(4):636-7
references.pmid: 17553287
references.citation: BMC Infect Dis. 2006 Jul 11;6:109
references.pmid: 16834781
references.citation: Clin Infect Dis. 2006 Sep 1;43(5):585-92
references.pmid: 16886150
references.citation: Eur J Clin Microbiol Infect Dis. 2007 Feb;26(2):147-9
references.pmid: 17216422
references.citation: J Clin Virol. 2006 Jan;35(1):99-102
references.pmid: 16257260
references.citation: Pediatrics. 2008 Mar;121(3):e631-7
references.pmid: 18310182
references.citation: J Clin Virol. 2008 Jul;42(3):233-43
references.pmid: 18406664
references.citation: Clin Microbiol Rev. 1996 Jan;9(1):18-33
references.pmid: 8665474
references.citation: Clin Microbiol Rev. 2008 Apr;21(2):291-304, table of contents
references.pmid: 18400798
references.citation: J Clin Virol. 2008 Jan;41(1):29-33
references.pmid: 18055252
references.citation: J Med Virol. 2003 Jun;70(2):228-39
references.pmid: 12696109
references.citation: Clin Microbiol Infect. 2007 May;13(5):504-9
references.pmid: 17263836
references.citation: Acta Clin Belg. 2008 Sep-Oct;63(5):329-34
references.pmid: 19186566
references.citation: J Infect Dis. 2006 Nov 1;194(9):1276-82
references.pmid: 17041854
references.citation: J Clin Microbiol. 2006 Sep;44(9):3231-5
references.pmid: 16954253