title: Preparation of acetochlor antibody and its application on immunoaffinity chromatography cleanup for residue determination in peanuts.
abstract: The preparation of an antibody against acetochlor and its application on immunoaffinity chromatography (IAC) cleanup was investigated. The hapten was synthesized by reacting acetochlor with 3-mercaptopropionic acid and then linked with a carrier protein by the carbodiimide method. After immunizing a rabbit with hapten-bovine serum albumin (BSA) conjugate, the obtained antiserum showed an IC(50) value of 0.2 ng/mL. By coupling the purified polyclonal antibody with CNBr-activated Sepharose 4B, an IAC column was prepared. The dynamic column capacity was 5560 ng/mL gel. The IAC was then used to clean up a peanut sample solution, followed by high-performance liquid chromatography (HPLC) separation and ultraviolet (UV) detection. The recoveries of acetochlor from spiked peanut samples at levels of 5-100 microg/kg ranged from 94.7 to 102.5%, with a variation coefficient of 2.6-8.0%, and the limits of detection and quantification were 1.5 and 5 microg/kg, respectively.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Shusheng
authors.initials: S
authors.lastname: Tang
mesh_terms: D015097:3-Mercaptopropionic Acid; D000818:Animals; D010367:Arachis / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D006241:Haptens / Q000276:immunology; D006540:Herbicides; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D014052:Toluidines / Q000032:analysis / Q000276:immunology*
chemical_list: D006241:Haptens; D006540:Herbicides; D007106:Immune Sera; D010573:Pesticide Residues; D014052:Toluidines; D012710:Serum Albumin, Bovine; C043377:acetochlor; D015097:3-Mercaptopropionic Acid