A new sample preparation method compatible with capillary electrophoresis and laser-induced fluorescence for improving detection of low levels of beta-lactoglobulin in infant foods.
Beta-lactoglobulin (betaLG) is the main allergenic protein in cow's milk and can cause allergy even when present at very low concentration. The aim of this work is to develop an innovative sample preparation method fully compatible with capillary electrophoresis and laser-induced fluorescence detection for improving the sensitivity when analyzing betaLG. Different types of baby food were on purpose contaminated with diverse dairy desserts and submitted to thermal treatment to simulate potential contamination at production. Sample preparation prior to CE analysis was performed by the classical extraction method and by the innovative one, and the results were compared. Analysis was performed by capillary electrophoresis with laser-induced fluorescence detection. The innovative method permitted to detect contaminations as low as 1 part of yoghurt in 10,000 parts of baby food.
Pelaez-Lorenzo
Cristina
C
Institute of Organic Chemistry (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
Diez-Masa
Jose Carlos
JC
Vasallo
Isabel
I
de Frutos
Mercedes
M
D019075:Electrophoresis, Capillary / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D007782:Lactoglobulins / Q000032:analysis* / Q000302:isolation & purification; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D007782:Lactoglobulins