Effect of dietary ascorbate on lipogenesis and lipolysis activities in black sea bream, Acanthopagrus schlegelii.
To assess the effect of dietary ascorbate on lipid metabolism, 1-year black sea bream (Acanthopagrus schlegelii) were reared on a casein-based purified diet and an ascorbate fortified diet (1,100 mg of L: -ascorbyl-2- monophosphate-Mg/kg diet). The fortified ascorbate was effectively incorporated into the fish body and elevated muscle carnitine content. Fortifications of dietary ascorbate depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancreas and intraperitoneal fat body. Starvation after feeding experiment activated carnitine palmitoyltransferase as a lipolysis enzyme in the hepatopancreas in both control and vitamin C(VC) groups, while the lipolysis activity was significantly higher in VC group. These results confirmed that dietary ascorbate depressed lipogenesis and activated lipolysis, i.e., influenced the lipid metabolism of black sea bream.
Ji
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College of Animal Science and Technology, Northwest A&F University, 712100, Shaanxi, China. jihong0405@hotmail.com.|Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan. jihong0405@hotmail.com.
Om
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Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.|Marine Finfish Production and Research Center, 22200, Terengganu, Malaysia.
Yoshimatsu
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Graduate School of Bioresources, Mie University, Mie, 514-8507, Japan.
Umino
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Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
Nakagawa
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Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
Sakamoto
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Oriental Yeast Co. Ltd., 8-2, Shinminato, Mihama-ku, Chiba, 281-0002, Japan.
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D001837:Body Weights and Measures; D002331:Carnitine / Q000032:analysis; D005527:Food, Fortified; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D043143:Hepatopancreas / Q000737:chemistry; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D050356:Lipid Metabolism / Q000187:drug effects*; D050155:Lipogenesis / Q000187:drug effects*; D008066:Lipolysis / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000737:chemistry; D011506:Proteins / Q000032:analysis; D021541:Sea Bream / Q000502:physiology*
D011506:Proteins; C011669:ascorbate-2-phosphate; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D005954:Glucosephosphate Dehydrogenase; D001205:Ascorbic Acid; D002331:Carnitine
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