title: Real-time bioluminescence imaging of viral pathogenesis.
abstract: Mouse models are used commonly to study viral infection and define viral and host determinants of infection and disease morbidity. Conventional studies of viral infection in mice rely upon euthanizing cohorts of animals at multiple time points to identify sites of infection, quantify viral titers, and determine host immune responses. This experimental paradigm precludes longitudinal studies of infection and response to treatment in the same animal and assumes that progression of infection and pharmacodynamics of therapeutic agents are identical in all mice. To enable repetitive, quantitative studies of viral infection in mouse models, we and others are using noninvasive bioluminescence imaging to track viral infection, dissemination, and effects of host immune mediators on disease. In this chapter, we detail experimental protocols for bioluminescence imaging of viral infections in living mice.
authors.affiliation: Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Luker
authors.forename: Gary D
authors.initials: GD
authors.lastname: Luker
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017930:Genes, Reporter; D054884:Host-Pathogen Interactions; D008156:Luciferases / Q000235:genetics; D049449:Luminescence; D051379:Mice; D014777:Virus Diseases / Q000473:pathology*; D014779:Virus Replication
chemical_list: D008156:Luciferases
references.citation: Mol Imaging. 2004 Apr;3(2):117-24
references.pmid: 15296676
references.citation: J Virol. 2002 Dec;76(23):12149-61
references.pmid: 12414955
references.citation: Mol Ther. 2005 Mar;11(3):435-43
references.pmid: 15727940
references.citation: J Virol. 2003 May;77(9):5333-8
references.pmid: 12692235
references.citation: J Immunol. 2008 Jan 1;180(1):483-91
references.pmid: 18097050
references.citation: Virology. 2005 Oct 25;341(2):284-300
references.pmid: 16095645
references.citation: Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):377-82
references.pmid: 11752410
references.citation: Mol Imaging. 2004 Jan;3(1):43-54
references.pmid: 15142411
references.citation: J Biomed Opt. 2005 Jul-Aug;10(4):41210
references.pmid: 16178634
references.citation: Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1702-7
references.pmid: 14755051
references.citation: Nat Methods. 2007 Aug;4(8):641-3
references.pmid: 17618292
references.citation: Mol Imaging. 2007 Jan-Feb;6(1):68-72
references.pmid: 17311766
references.citation: Virology. 2005 Mar 30;334(1):28-40
references.pmid: 15749120