Synthesis of novel isoluminol probes and their use in rapid bacterial assays.
Rapid assays for bacteria have been developed utilizing novel LysLysLys-isoluminol (14) and GluGlu-isoluminol (16) probes that have been derived from peptides which potentially mimic bacteriophage attachment protein binding regions. Compared to two conventional methods that are widely used, namely nucleic acid probes and polymerase chain reaction (PCR) assays, these types of probes may eventually have certain advantages, such as high sensitivity, and short preparation and assay time.
Neelakantan
Sundar
S
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY-40536, USA.
Surjawan
Iwan
I
Karacelik
Huseyin
H
Hicks
Clair L
CL
Crooks
Peter A
PA
D001419:Bacteria / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000378:metabolism; D004151:Dipeptides / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008165:Luminol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D001426:Bacterial Proteins; D004151:Dipeptides; D005456:Fluorescent Dyes; D009842:Oligopeptides; C545113:glutamyl-glutamyl-isoluminol; C545112:lysyl-lysyl-lysyl-isoluminol; C032487:isoluminol; D008165:Luminol