Sevoflurane postconditioning protects isolated rat hearts against ischemia-reperfusion injury: the role of radical oxygen species, extracellular signal-related kinases 1/2 and mitochondrial permeability transition pore.
The roles of reactive oxygen species (ROS), extracellular signal-regulated kinase 1/2 (ERK 1/2) and mitochondrial permeability transition pore (mPTP) in sevoflurane postconditioning induced cardioprotection against ischemia-reperfusion injury in Langendorff rat hearts were investigated. When compared with the unprotected hearts subjected to 30 min of ischemia followed by 1 h of reperfusion, exposure of 3% sevoflurane during the first 15 min of reperfusion significantly improved functional recovery, decreased infarct size, reduced lactate dehydrogenase and creatine kinase-MB release, and reduced myocardial malondialdehyde production. However, these protective effects were abolished in the presence of either ROS scavenger N-acetylcysteine or ERK 1/2 inhibitor PD98059, and accompanied by prevention of ERK 1/2 phosphorylation and elimination of inhibitory effect on mPTP opening. These findings suggested that sevoflurane postconditioning protected isolated rat hearts against ischemia-reperfusion injury via the recruitment of the ROS-ERK 1/2-mPTP signaling cascade.
Yao
Yun-Tai
YT
Department of Anesthesiology, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 100037 Beijing, China.
Li
Li-Huan
LH
Chen
Lei
L
Wang
Wei-Peng
WP
Li
Li-Bing
LB
Gao
Chang-Qing
CQ
D000818:Animals; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D048370:MAP Kinase Kinase 2 / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008738:Methyl Ethers / Q000494:pharmacology*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D009203:Myocardial Infarction / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D009243:NAD / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015427:Reperfusion Injury / Q000150:complications / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D000077149:Sevoflurane
D008738:Methyl Ethers; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017382:Reactive Oxygen Species; D009243:NAD; D000077149:Sevoflurane; D008315:Malondialdehyde; D048052:Mitogen-Activated Protein Kinase 3; D048370:MAP Kinase Kinase 2
Am J Physiol Heart Circ Physiol. 2005 Oct;289(4):H1618-26
15937101
Br J Anaesth. 2005 Aug;95(2):140-52
15939730
Can J Anaesth. 2006 Feb;53(2):174-82
16434759
Acta Pharmacol Sin. 2008 Aug;29(8):931-41
18664326
Clin Exp Pharmacol Physiol. 2008 Sep;35(9):1043-51
18505453
Am J Physiol Heart Circ Physiol. 2003 Feb;284(2):H566-74
12414448
FEBS Lett. 2001 Mar 9;492(1-2):4-8
11248227
Cardiovasc Res. 2006 Oct 1;72(1):152-62
16901477
Anesthesiology. 2002 Jul;97(1):42-9
12131102
J Mol Cell Cardiol. 2004 Mar;36(3):411-21
15010280
Cardiovasc Res. 2004 Apr 1;62(1):74-85
15023554
Am J Physiol Heart Circ Physiol. 2005 Apr;288(4):H1900-8
15563525
Anesth Analg. 2003 Apr;96(4):949-955
12651639
Am J Physiol Renal Physiol. 2006 Oct;291(4):F840-55
16705147
J Biol Chem. 1995 Jan 20;270(3):987-90
7836418
J Biol Chem. 2001 Jan 26;276(4):2571-5
11073947
Circ Res. 2000 Sep 15;87(6):460-6
10988237
Anesthesiology. 2005 Jan;102(1):102-9
15618793
Anesth Analg. 2007 Aug;105(2):316-24
17646483
Anesth Analg. 2005 Nov;101(5):1275-1287
16243980
Anesthesiology. 2003 Oct;99(4):826-33
14508313
Circ Res. 2001 Aug 3;89(3):273-8
11485978
J Biomed Sci. 2004 Jan-Feb;11(1):11-8
14730205
Anesth Analg. 2005 Mar;100(3):610-616
15728039
Anesthesiology. 2002 Apr;96(4):934-40
11964602
Anesthesiology. 2003 Aug;99(2):314-23
12883404
Circ Res. 2002 Mar 8;90(4):390-7
11884367
Cardiovasc Res. 2004 Feb 15;61(3):461-70
14962477
Anesth Analg. 2005 Jan;100(1):46-53
15616050
Anesth Analg. 2007 Dec;105(6):1534-9, table of contents
18042844
J Mol Cell Cardiol. 2006 Aug;41(2):248-55
16697003
Am J Physiol. 1999 May;276(5):H1468-81
10330229
Life Sci. 2007 Sep 22;81(15):1223-7
17915258
Anesthesiology. 2002 Dec;97(6):1485-90
12459675
Circ Res. 2003 May 2;92(8):873-80
12663490
J Cardiothorac Vasc Anesth. 2004 Jun;18(3):269-74
15232804
Anesth Analg. 2005 Nov;101(5):1252-1260
16243977
Anesthesiology. 2004 Dec;101(6):1372-80
15564945
J Clin Invest. 2004 Jun;113(11):1535-49
15173880
Cardiovasc Res. 2004 Aug 1;63(2):305-12
15249188
Antioxid Redox Signal. 2004 Apr;6(2):439-48
15025946
Cardiovasc Res. 2004 Feb 15;61(3):448-60
14962476