title: Temporal and spatial patterns of gene expression in skeletal muscles in response to swim training in adult zebrafish (Danio rerio).
abstract: In adult zebrafish, 4 weeks of exercise training is known to induce an increase in mitochondrial enzymes such as citrate synthase (CS) when determined in mixed (red and white) muscle. However, this remodeling is not accompanied by changes in PGC-1alpha mRNA, a potent inducer of mitochondrial biogenesis in mammals. To further understand this response, we examined absolute and relative changes in red muscle area by histochemistry after 4 weeks of swim training. We also examined fiber-type specific responses in the expression of metabolic genes and putative regulators in red and white muscle of adult zebrafish at 1 and 8 weeks of training and in recovery from a single bout of exercise. Total red muscle area was unaltered after 4 weeks of training. The mRNA expression of CS was unaffected in red muscle, while it was increased in white muscle after 1 week of training and remained elevated at 8 weeks of training, suggesting an increase in oxidative capacity of this fiber type. In contrast, PGC-1alpha mRNA was elevated in both muscles only after 1 week of training. In both muscles, an acute bout of exercise rapidly (within 0-2 h post-exercise) induced PGC-1alpha mRNA and a delayed (24 h) increase in CS mRNA post-exercise. These results suggest complex temporal and spatial adaptive molecular responses to exercise in the skeletal muscles of zebrafish.
authors.affiliation: Department of Biology, McMaster University, Hamilton, ON, L8S 4K1, Canada. clemoin@univmail.cis.mcmaster.ca
authors.forename: Christophe M R
authors.initials: CM
authors.lastname: LeMoine
authors.forename: Paul M
authors.initials: PM
authors.lastname: Craig
authors.forename: Kalindi
authors.initials: K
authors.lastname: Dhekney
authors.forename: Julie J
authors.initials: JJ
authors.lastname: Kim
authors.forename: Grant B
authors.initials: GB
authors.lastname: McClelland
mesh_terms: D042964:Acyl-CoA Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D049628:Body Size; D002950:Citrate (si)-Synthase / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism*; D009928:Organ Specificity; D048068:PPAR-beta / Q000235:genetics / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013550:Swimming / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D009124:Muscle Proteins; D048068:PPAR-beta; D020033:Protein Isoforms; D012333:RNA, Messenger; D014157:Transcription Factors; D029961:Zebrafish Proteins; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1; D042964:Acyl-CoA Dehydrogenase; D002950:Citrate (si)-Synthase
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