title: Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats.
abstract: Immunizing animals in the wild against Brucella (B.) abortus is essential to control bovine brucellosis because cattle can get the disease through close contact with infected wildlife. The aim of this experiment was to evaluate the effectiveness of the B. abortus strain RB51 vaccine in protecting infection as well as vertical transmission in Sprague-Dawley (SD) rats against B. abortus biotype 1. Virgin female SD rats (n = 48) two months of age were divided into two groups: one group (n = 24) received RB51 vaccine intraperitoneally with 3 x 10(10) colony forming units (CFU) and the other group (n = 24) was used as non-vaccinated control. Non-vaccinated and RB51-vaccinated rats were challenged with 1.5 x 10(9) CFU of virulent B. abortus biotype 1 six weeks after vaccination. Three weeks after challenge, all rats were bred. Verification of RB51-vaccine induced protection in SD rats was determined by bacteriological, serological and molecular screening of maternal and fetal tissues at necropsy. The RB51 vaccine elicited 81.25% protection in SD rats against infection with B. abortus biotype 1. Offspring from rats vaccinated with RB51 had a decreased (p < 0.05) prevalence of vertical transmission of B. abortus biotype 1 compared to the offspring from non-vaccinated rats (20.23% and 87.50%, respectively). This is the first report of RB51 vaccination efficacy against the vertical transmission of B. abortus in the SD rat model.
authors.affiliation: Korean Zoonoses Research Institute, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Md Ariful
authors.initials: MA
authors.lastname: Islam
authors.forename: Mst Minara
authors.initials: MM
authors.lastname: Khatun
authors.forename: Byeong-Kirl
authors.initials: BK
authors.lastname: Baek
authors.forename: Sung-Il
authors.initials: SI
authors.lastname: Lee
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology / Q000592:standards*; D001724:Birth Weight; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control* / Q000635:transmission*; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control*; D008098:Litter Size; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D051381:Rats; D017207:Rats, Sprague-Dawley; D016019:Survival Analysis
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines
references.citation: Vet Rec. 1978 Aug 19;103(8):149-53
references.pmid: 695262
references.citation: Rev Sci Tech. 2002 Aug;21(2):277-86
references.pmid: 11974615
references.citation: Vaccine. 2006 Jun 19;24(25):5327-34
references.pmid: 16713034
references.citation: Res Vet Sci. 2000 Oct;69(2):135-40
references.pmid: 11020364
references.citation: Vet Microbiol. 1991 Jul;28(2):171-88
references.pmid: 1908158
references.citation: J Am Vet Med Assoc. 1981 Dec 1;179(11):1105-12
references.pmid: 6799465
references.citation: Vet Rec. 2008 Apr 26;162(17):556-7
references.pmid: 18441353
references.citation: Infect Immun. 1995 Jan;63(1):264-70
references.pmid: 7806364
references.citation: J Wildl Dis. 1994 Jul;30(3):445-6
references.pmid: 7933293
references.citation: J Clin Microbiol. 2008 Oct;46(10):3484-7
references.pmid: 18716225
references.citation: Vet Microbiol. 2002 Dec 20;90(1-4):533-44
references.pmid: 12414169
references.citation: Vet Immunol Immunopathol. 1995 Feb;44(3-4):223-35
references.pmid: 7747403
references.citation: Infect Immun. 1996 Mar;64(3):1030-4
references.pmid: 8641754
references.citation: Infect Immun. 2003 May;71(5):2326-30
references.pmid: 12704101
references.citation: Vet Res. 2004 Jan-Feb;35(1):1-38
references.pmid: 15099501
references.citation: N Engl J Med. 2005 Jun 2;352(22):2325-36
references.pmid: 15930423
references.citation: Clin Infect Dis. 1995 Aug;21(2):283-9; quiz 290
references.pmid: 8562733
references.citation: Res Vet Sci. 2003 Feb;74(1):17-22
references.pmid: 12507562
references.citation: Vet Rec. 1982 Dec 18;111(25-26):578-9
references.pmid: 7157620
references.citation: Emerg Infect Dis. 1997 Apr-Jun;3(2):213-21
references.pmid: 9204307
references.citation: Infect Immun. 1993 Jan;61(1):124-34
references.pmid: 8418034
references.citation: Can J Vet Res. 2005 Oct;69(4):305-8
references.pmid: 16479730
references.citation: J Clin Microbiol. 1994 Apr;32(4):1065-6
references.pmid: 8027313
references.citation: Am J Vet Res. 1997 May;58(5):472-7
references.pmid: 9140553
references.citation: Med Parazitol (Mosk). 1989 May-Jun;(3):73-7
references.pmid: 2674644
references.citation: Infect Immun. 1994 Nov;62(11):4990-6
references.pmid: 7927779
references.citation: J Am Vet Med Assoc. 1986 Apr 15;188(8):867-9
references.pmid: 3710878
references.citation: Am J Vet Res. 1993 Oct;54(10):1591-7
references.pmid: 8250382
references.citation: Ann Rech Vet. 1982;13(4):341-9
references.pmid: 6821020
references.citation: Am J Vet Res. 1996 Aug;57(8):1153-6
references.pmid: 8836366
references.citation: J Appl Microbiol. 2005;98(6):1270-81
references.pmid: 15916641
references.citation: Am J Vet Res. 1992 Oct;53(10):1881-8
references.pmid: 1456536
references.citation: J Korean Med Sci. 2005 Dec;20(6):941-6
references.pmid: 16361801
references.citation: Am J Vet Res. 1998 Dec;59(12):1575-8
references.pmid: 9858409