Parallel expression evolution of oxidative stress-related genes in fiber from wild and domesticated diploid and polyploid cotton (Gossypium).
BACKGROUND
Reactive oxygen species (ROS) play a prominent role in signal transduction and cellular homeostasis in plants. However, imbalances between generation and elimination of ROS can give rise to oxidative stress in growing cells. Because ROS are important to cell growth, ROS modulation could be responsive to natural or human-mediated selection pressure in plants. To study the evolution of oxidative stress related genes in a single plant cell, we conducted comparative expression profiling analyses of the elongated seed trichomes ("fibers") of cotton (Gossypium), using a phylogenetic approach.


RESULTS
We measured expression changes during diploid progenitor species divergence, allopolyploid formation and parallel domestication of diploid and allopolyploid species, using a microarray platform that interrogates 42,429 unigenes. The distribution of differentially expressed genes in progenitor diploid species revealed significant up-regulation of ROS scavenging and potential signaling processes in domesticated G. arboreum. Similarly, in two independently domesticated allopolyploid species (G. barbadense and G. hirsutum) antioxidant genes were substantially up-regulated in comparison to antecedent wild forms. In contrast, analyses of three wild allopolyploid species indicate that genomic merger and ancient allopolyploid formation had no significant influences on regulation of ROS related genes. Remarkably, many of the ROS-related processes diagnosed as possible targets of selection were shared among diploid and allopolyploid cultigens, but involved different sets of antioxidant genes.


CONCLUSIONS
Our data suggests that parallel human selection for enhanced fiber growth in several geographically widely dispersed species of domesticated cotton resulted in similar and overlapping metabolic transformations of the manner in which cellular redox levels have become modulated.
Chaudhary
Bhupendra
B
Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, Iowa 50011, USA. bhupendra@gbu.ac.in
Hovav
Ran
R
Flagel
Lex
L
Mittler
Ron
R
Wendel
Jonathan F
JF
D032561:Cotton Fiber; D004171:Diploidy; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D003368:Gossypium / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000235:genetics*; D010802:Phylogeny; D011123:Polyploidy; D018749:RNA, Plant / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018749:RNA, Plant; D017382:Reactive Oxygen Species
EMBO J. 2000 Dec 15;19(24):6770-7
11118212
BMC Plant Biol. 2007 May 16;7:22
17506877
Nature. 2004 Feb 26;427(6977):858-61
14985766
Plant Physiol. 2004 Oct;136(2):3114-23; discussion 3001
15466236
Trends Plant Sci. 2005 Jul;10(7):339-46
15953753
Am J Bot. 2002 Apr;89(4):707-25
21665671
FEBS Lett. 2002 Oct 9;529(2-3):198-202
12372600
New Phytol. 2007;175(3):462-471
17635221
Trends Plant Sci. 2003 Jul;8(7):335-42
12878018
Plant Physiol. 2005 Oct;139(2):806-21
16183842
Planta. 2008 Jul;228(2):281-92
18425534
Mol Biol Evol. 2008 Mar;25(3):507-16
18187560
Planta. 1998 Oct;206(3):378-87
9763707
J Biol Chem. 2004 Mar 19;279(12):11736-43
14722088
Plant J. 2003 Apr;34(2):187-203
12694594
PLoS Genet. 2008 Feb;4(2):e25
18248099
Trends Plant Sci. 2001 Jun;6(6):262-7
11378468
Methods Mol Biol. 2009;527:3-19, ix
19241001
Plant J. 2001 Feb;25(4):407-16
11260497
Trends Plant Sci. 2000 May;5(5):193-8
10785664
Curr Biol. 1999 Jul 15;9(14):775-8
10421577
Plant Physiol. 2002 Jun;129(2):838-53
12068123
BMC Genomics. 2007 Mar 27;8:81
17389046
Biochem J. 1998 Jun 1;332 ( Pt 2):507-15
9601081
Trends Plant Sci. 2002 Sep;7(9):405-10
12234732
Plant Cell. 2005 Jan;17(1):268-81
15608336
Trends Plant Sci. 2000 Jun;5(6):241-6
10838614
Evol Dev. 2008 Sep-Oct;10(5):567-82
18803775
Plant Physiol. 1999 Mar;119(3):849-58
10069824
EMBO J. 1997 Aug 15;16(16):4806-16
9305623
Anal Biochem. 1994 Nov 15;223(1):7-12
7535022
Genetics. 2006 Aug;173(4):2199-210
16702414
Annu Rev Plant Biol. 2004;55:373-99
15377225
Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):12191-6
16873546
J Biol Chem. 2003 Oct 3;278(40):38921-5
12885779
Plant Physiol. 2001 Apr;125(4):1591-602
11299341
Plant Physiol. 2007 Aug;144(4):1777-85
17556505
Evol Dev. 2001 Jan-Feb;3(1):3-17
11256432
Trends Plant Sci. 2004 Oct;9(10):490-8
15465684
Science. 2007 Jun 29;316(5833):1890-3
17600214
Nature. 2003 Mar 27;422(6930):442-6
12660786
Cell Mol Life Sci. 2000 May;57(5):779-95
10892343
Science. 2008 Feb 29;319(5867):1241-4
18309082