Generation of stable co-cultures of vascular cells in a honeycomb alginate scaffold.
Scaffold-guided vascular tissue engineering has been investigated as a means to generate functional and transplantable vascular tissue grafts that increase the efficacy of cell-based therapeutic strategies in regenerative medicine. In this study, we employed confocal microscopy and three-dimensional reconstruction to assess the engraftment and growth potential of vascular cells within an alginate scaffold with aligned pores. We fabricated honeycomb alginate scaffolds with aligned pores, whose surface was immobilized with fibronectin and subsequently coated with matrigel. Endothelial cells were seeded into aligned pore scaffolds in the presence and absence of human smooth muscle cells. We showed that endothelial cells seeded into alginate scaffolds attach on the surface of aligned pores in vitro, giving rise to stable co-cultures of vascular cells. Moreover, the three-dimensional alginate depots containing the cells were exposed to laminar flow in order to recapitulate physiological shear stress found in the vasculature in vivo. After the flow exposure, the scaffold remained intact and some cells remained adherent to the scaffold and aligned in the flow direction. These studies demonstrate that alginate scaffolds provide a suitable matrix for establishing durable angiogenic modules that may ultimately enhance organ revascularization.
Yamamoto
Masaya
M
Department of Genetics Medicine, Weill Medical College of Cornell University, New York, New York, USA.
James
Daylon
D
Li
Hui
H
Butler
Jason
J
Rafii
Shahin
S
Rabbany
Sina
S
D000464:Alginates; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D018919:Neovascularization, Physiologic; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
Biomacromolecules. 2004 Sep-Oct;5(5):1720-7
15360280
Biotechnol Bioeng. 1998 Jan 5;57(1):46-54
10099177
Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19288-93
19036927
Angew Chem Int Ed Engl. 2007;46(25):4689-92
17503408
Pharm Res. 2003 Aug;20(8):1103-12
12948005
J Biomed Mater Res. 2001 Sep 5;56(3):368-75
11372054
Br J Surg. 2005 Jun;92(6):665-72
15912490
J Mater Sci Mater Med. 1999 Dec;10(12):773-7
15347949
J Biomed Mater Res. 2000 Sep 5;51(3):376-82
10880079
Nat Rev Drug Discov. 2002 Mar;1(3):177-86
12120502
Curr Top Dev Biol. 2004;64:181-205
15563948
Biomaterials. 2001 Dec;22(23):3169-78
11603589
J Biomed Mater Res. 2000 Dec 5;52(3):576
11007628
Tissue Eng. 2000 Feb;6(1):39-44
10941199
Nat Biotechnol. 2005 Jan;23(1):47-55
15637621
Biotechnol Bioeng. 2008 Apr 1;99(5):1230-40
17929323
Curr Opin Biotechnol. 2004 Oct;15(5):381-2
15464365
J Biomed Mater Res. 2000 Aug;51(2):233-40
10825223
J Vasc Surg. 2005 Feb;41(2):349-54
15768021
Eur J Vasc Endovasc Surg. 1996 Jul;12(1):46-53
8696896
Biomaterials. 1998 Aug;19(15):1405-12
9758040
Science. 1993 May 14;260(5110):920-6
8493529
Nature. 2004 Apr 1;428(6982):487-92
15057821
Biosens Bioelectron. 2007 Jun 15;22(12):2803-11
17240132
Clin Plast Surg. 2003 Oct;30(4):573-9
14621305
Am J Physiol Heart Circ Physiol. 2005 Apr;288(4):H1915-24
15576436
Artif Cells Blood Substit Immobil Biotechnol. 1996 Jan;24(1):51-63
8714719
Clin Plast Surg. 2003 Oct;30(4):589-99
14621307
J Biomed Mater Res A. 2003 Nov 1;67(2):647-57
14566809
Biorheology. 1984;21(4):617-30
6487771
J Biol Chem. 2004 Mar 19;279(12):11760-6
14660586
Anal Biochem. 1992 Nov 15;207(1):186-92
1489093
Tissue Eng. 2007 Aug;13(8):1837-44
17590149
J Vasc Surg. 2007 Jun;45 Suppl A:A104-15
17544031