DNA sensor development based on multi-wall carbon nanotubes for label-free influenza virus (type A) detection.
This paper describes the DNA immobilization using carbon multi-walled nanotubes (MWCNTs) for direct and label-free detection of influenza virus (type A). The DNA probe was attached on the sensor surface by means of covalent bonding between the amine and phosphate groups of the DNA sequence. The interaction between the DNA probe and the MWCNTs were characterized by Fourier Transform Infrared (FTIR) spectrometry, Raman spectra. The hybridization of the DNA probe and the target DNA were detected by changes in the conductance on the surface of sensors leading to the change in the output signal of the system. The results show that the DNA sensor can detect as low as 0.5 nM of the target DNA samples; the response time of DNA sensor is approximately 4 min.
Tam
Phuong Dinh
PD
Hanoi Advanced School of Science and Technology, Hanoi University of Technology, Vietnam. tampd-hast@mail.hut.edu.vn
Van Hieu
Nguyen
N
Chien
Nguyen Duc
ND
Le
Anh-Tuan
AT
Anh Tuan
Mai
M
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D009980:Influenza A virus / Q000737:chemistry* / Q000302:isolation & purification*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors
D015342:DNA Probes; D037742:Nanotubes, Carbon; D004247:DNA