title: [Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion].
abstract: OBJECTIVE
  To investigate the expression of intercellular adhesion molecular-1 (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization (FISH) and its relationship with the contusion interval.
  
  
  METHODS
  To make the contusion models with rats skeletal muscle. The samples were taken to extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control group after contusion respectively. FISH was performed on frozen section samples and the sections were observed using LSCM.
  
  
  RESULTS
  The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times the level of control group at 18 h and then increased again.
  
  
  CONCLUSIONS
  The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.
authors.affiliation: School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. peiming5992@163.com
authors.forename: Ming
authors.initials: M
authors.lastname: Pei
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Sun
authors.forename: Cai-Rong
authors.initials: CR
authors.lastname: Gao
mesh_terms: D000818:Animals; D003288:Contusions / Q000378:metabolism* / Q000473:pathology; D005260:Female; D049429:Forensic Pathology; D006614:Hindlimb / Q000293:injuries*; D017404:In Situ Hybridization, Fluorescence; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014945:Wound Healing
chemical_list: D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1