title: Isolation of deletion alleles by G4 DNA-induced mutagenesis.
abstract: Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.
authors.affiliation: Hubrecht Institute-Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Utrecht, The Netherlands.
authors.forename: Daphne B
authors.initials: DB
authors.lastname: Pontier
authors.forename: Evelien
authors.initials: E
authors.lastname: Kruisselbrink
authors.forename: Victor
authors.initials: V
authors.lastname: Guryev
authors.forename: Marcel
authors.initials: M
authors.lastname: Tijsterman
mesh_terms: D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D054856:G-Quadruplexes; D017353:Gene Deletion; D016296:Mutagenesis
chemical_list: D004247:DNA
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