title: The impact of defined polyglycolide scaffold structure on the proliferation of gingival fibroblasts in vitro: a pilot study.
abstract: OBJECTIVE
  The postoperative apical growth of epithelium on teeth is a common complication of surgical periodontal therapy. Absorbable textile scaffolds, placed between the operated tooth and its nearby tissues, are considered to serve as a mechanical barrier and prevent undesired apical epithelial growth. They allow local regeneration and the new formation of human gingival fibroblasts when the scaffolds are colonized with autologous cells. The aim of this study was to examine the proliferation behavior of human gingival fibroblasts on polyglycolic acid (PGA) fleeces with various structural characteristics.
  
  
  METHODS
  The PGA fleeces varied in the following characteristics: thickness (1.0-5.0 mm), porosity (85%-98%), mass-area ratio (56.6-166.9 g/m(2)), and texture. The proliferation of incorporated autologous cells was analyzed by determining the number of live cells, the total absorbed protein, and the degree of cell proliferation.
  
  
  RESULTS
  The results showed that fleece made from Knit-de-Knit textured fibers with a porosity of about 90%, a mass-area between 58 and 84 g/m(2), and a thickness of 1.5 mm was most suitable as a scaffold structure. This fleece showed the highest cell proliferation rate and was superior to fleeces with other structural characteristics.
  
  
  CONCLUSIONS
  Textile PGA fleece seems to be suitable as a scaffold structure for human gingival fibroblasts. The structural parameters of the fleece have a significant influence on the proliferation of the cells.
authors.affiliation: Department of Conservative Dentistry, Periodontology, and Preventive Dentistry, University Hospital Aachen, Aachen, Germany. fbaeumchen@ukaachen.de
authors.forename: Frank
authors.initials: F
authors.lastname: Bäumchen
authors.forename: Ralf
authors.initials: R
authors.lastname: Smeets
authors.forename: Daniel
authors.initials: D
authors.lastname: Koch
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Gräber
mesh_terms: D000042:Absorption; D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004867:Equipment Design; D005347:Fibroblasts / Q000502:physiology*; D005881:Gingiva / Q000166:cytology*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010865:Pilot Projects; D011100:Polyglycolic Acid / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000737:chemistry; D013242:Sterilization / Q000379:methods; D013499:Surface Properties; D013997:Time Factors; D054457:Tissue Scaffolds; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011506:Proteins; D014867:Water; D011100:Polyglycolic Acid