Simultaneous analyses of vitreous levels of 27 cytokines in eyes with retinopathy of prematurity.
OBJECTIVE
To determine the vitreous levels of 27 types of cytokines in eyes with retinopathy of prematurity (ROP).


METHODS
Retrospective case-control study.


METHODS
Twenty-seven eyes of 19 infants with stage 4 ROP were studied. Six eyes of 5 patients with congenital cataract who underwent lensectomy were used as controls.


METHODS
The ROP eyes were divided into 2 groups according to vascular activity: 12 eyes with vascularly active ROP and 15 eyes with vascularly inactive ROP. Undiluted vitreous samples were collected, and the vitreous concentrations of 27 types of cytokines were determined by a multiplex bead analysis system: interleukin (IL)-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, Eotaxin, fibroblast growth factor (FGF) basic, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), interferon-r, interferon-gamma-inducible protein (IP)-10, monocyte chemoattractant protein 1, macrophage inflammatory protein (MIP)-1a, MIP-1b, platelet-derived growth factor bb, regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor alpha, and vascular endothelial growth factor (VEGF).


METHODS
The vitreous levels of the 27 types of cytokines and a comparison of the levels in the 3 groups.


RESULTS
The postmenstrual age at vitrectomy was significantly younger in the vascularly active ROP eyes than in vascularly inactive ROP eyes. The cytokines that had significantly different vitreous levels among the 3 groups were: IL-6, IL-7, IL-10, IL-15, Eotaxin, FGF basic, G-CSF, GM-CSF, IP-10, RANTES, and VEGF (P<0.05). The vitreous levels of IL-6, IL-7, IL-15, Eotaxin, G-CSF, IP-10, and RANTES were significantly higher (P<0.05) in both vascularly active and inactive ROP eyes than in control eyes, whereas the vitreous level of VEGF was significantly higher (P<0.05) only in vascularly active ROP eyes than in control eyes. There was a significantly negative correlation (r = -0.382; P = 0.0495) between the VEGF level and the postmenstrual age at vitrectomy.


CONCLUSIONS
These results indicate that, although cytokines other than VEGF may be involved in the pathologic changes in eyes with ROP, VEGF is likely to have the strongest correlation with the vascular activity in ROP eyes among these cytokines.
Sato
Tatsuhiko
T
Department of Applied Visual Science, Osaka University Medical School, Osaka, Japan.
Kusaka
Shunji
S
Shimojo
Hiroshi
H
Fujikado
Takashi
T
D016022:Case-Control Studies; D002386:Cataract / Q000151:congenital / Q000378:metabolism; D002387:Cataract Extraction; D002675:Child, Preschool; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D008297:Male; D012178:Retinopathy of Prematurity / Q000378:metabolism*; D012189:Retrospective Studies; D014822:Vitreous Body / Q000378:metabolism*
D016207:Cytokines