Desmoglein 3 is overexpressed in inverted papilloma and squamous cell carcinoma of sinonasal cavity.
OBJECTIVE
We sought to investigate the role of desmoglein 3 in pathogenesis of sinonasal inverted papilloma (IP) and its malignant transformation.


METHODS
Fifteen subjects with sinonasal IP and 15 subjects of normal sphenoid sinus mucosa were enrolled. Each specimen was divided into two portions: one for mRNA expression analysis by real-time polymerase chain reaction, and the other for detection of targeted proteins by immunohistochemistry analysis. In addition, another 10 cases of IP with squamous cell carcinoma (SCC) were added for immunohistochemistry analysis.


RESULTS
The mRNA expression level of desmoglein 3 was significantly higher in IP tissues than in the normal sinus mucosa (P < .001). In immunohistochemistry study, desmoglein 3 was detected in plasma membrane areas of IP and IP with SCC tissues, but no obvious expression was found in normal sinus mucosa (total score; both P < .001). Positive desmoglein 3 staining was strongly present in nearly all malignant transformation areas of IP with SCC cases (90%), but only in scattered areas of some cases of IP (53%) (total score; P < .001).


CONCLUSIONS
Desmoglein 3 was overexpressed in IP and IP with SCC, and the overexpression was correlated with malignant transformation of IP. It may provide valuable insight into the pathobiology of this disease, and can potentially provide a venue to predict malignant transformation in sinonasal IP.
Huang
Chi-Che
CC
Department of Otolaryngoloy-Head and Neck Surgery, Chang Gung Memorial Hospital and Chang Gung University, Tao-Yuan, Taiwan.
Lee
Ta-Jen
TJ
Chang
Po-Hung
PH
Lee
Yun-Shien
YS
Chuang
Chi-Cheng
CC
Jhang
You-Jia
YJ
Chen
Yi-Wei
YW
Chen
Chiang-Wen
CW
Tsai
Chi-Neu
CN
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D051184:Desmoglein 3 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009669:Nose Neoplasms / Q000378:metabolism*; D018308:Papilloma, Inverted / Q000378:metabolism*; D010255:Paranasal Sinus Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051184:Desmoglein 3; D012333:RNA, Messenger