Structural optimization for heat detection of DNA thermosequencing platform using finite element analysis.
For the past three decades, Sanger's method has been the primary DNA sequencing technology; however, inherent limitations in cost and complexity have limited its usage in personalized medicine and ecological studies. A new technology called "thermosequencing" can potentially reduce both the cost and complexity of DNA sequencing by using a microfluidic platform [Esfandyarpour, Pease, and Davis, J. Vac. Sci. Technol. B26, 661 (2008)]. To optimize the efficiency of the technology, finite element analysis was used to model the thermosequencing system by simulating the DNA incorporation reaction series and the resulting product concentration and heat production. Different models of the thermosequencing platform were created to simulate the effects of the materials surrounding the system, to optimize the geometry of the system, and to concentrate reaction heat into specific regions for detection in the real system. The resulting concentrations of reaction products were used to calibrate the reaction speed and to design the heat sensors in the thermosequencing technology. We recommend a modified gated structure for the microfluidic detection platform by using control valves and show how this new platform could dramatically improve the detection efficiency.
Esfandyarpour
Hesaam
H
Zheng
Bo
B
Pease
R Fabian W
RF
Davis
Ronald W
RW
J Vac Sci Technol B Microelectron Nanometer Struct Process Meas Phenom. 2009 Dec 4;27(6):3099-3103
20467573
Biochim Biophys Acta. 1996 Oct 24;1291(2):115-21
8898871
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14719-24
14623953
Nucleic Acids Res. 2004 Dec 02;32(21):e166
15576673
Nature. 1986 Jun 12-18;321(6071):674-9
3713851
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7
271968
Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9517-22
15210951