title: Quantitative site-specific analysis of protein glycosylation by LC-MS using different glycopeptide-enrichment strategies.
abstract: A common technique for analysis of protein glycosylation is HPLC coupled to mass spectrometry (LC-MS). However, analysis is challenging due to a low abundance of glycopeptides in complex protein digests, microheterogeneity at the glycosylation site, ion suppression effects, and competition for ionization by coeluting peptides. Specific sample preparation is necessary for a comprehensive and site-specific glycosylation analysis by MS. In this study we qualitatively compared hydrophilic interaction chromatography (HILIC) and hydrazine chemistry for the enrichment of all N-linked glycopeptides and titanium dioxide for capturing sialylated glycopeptides from a complex peptide mixture. Bare silica, microcrystalline cellulose, amino-, amide- (TSKgel Amide-80), and sulfobetaine-(ZIC-HILIC) bonded phases were evaluated for HILIC enrichment. The experiments revealed that ZIC-HILIC and TSKgel Amide-80 are very specific for capturing glycopeptides under optimized conditions. Quantitative analysis of N-glycosidase F-released and 2-aminobenzamide-labeled glycans of a ZIC-HILIC-enriched monoclonal antibody demonstrated that glycopeptides could be enriched without bias for particular glycan structures and without significant losses. Sialylated glycopeptides could be efficiently enriched by titanium dioxide and in addition to HILIC both methods enable a comprehensive analysis of protein glycosylation by MS. Enrichment of N-linked glycopeptides by hydrazine chemistry resulted in lower peptide recovery using a more complex enrichment scheme.
authors.affiliation: Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Jessica
authors.initials: J
authors.lastname: Wohlgemuth
authors.forename: Michael
authors.initials: M
authors.lastname: Karas
authors.forename: Thomas
authors.initials: T
authors.lastname: Eichhorn
authors.forename: Robertus
authors.initials: R
authors.lastname: Hendriks
authors.forename: Sven
authors.initials: S
authors.lastname: Andrecht
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006020:Glycopeptides / Q000032:analysis* / Q000302:isolation & purification; D006031:Glycosylation; D006834:Hydrazines / Q000737:chemistry; D009439:Neuraminidase / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014025:Titanium / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000737:chemistry
chemical_list: D006020:Glycopeptides; D006834:Hydrazines; D062367:ortho-Aminobenzoates; C009495:titanium dioxide; C029424:hydrazine; D014025:Titanium; D009439:Neuraminidase; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; C000219:anthranilamide