Upregulation of 5-HT2C receptors in hippocampus of pilocarpine-induced epileptic rats: antagonism by Bacopa monnieri.
Emotional disturbances, depressive mood, anxiety, aggressive behavior, and memory impairment are the common psychiatric features associated with temporal lobe epilepsy (TLE). The present study was carried out to investigate the role of Bacopa monnieri extract in hippocampus of pilocarpine-induced temporal lobe epileptic rats through the 5-HT(2C) receptor in relation to depression. Our results showed upregulation of 5-HT(2C) receptors with a decreased affinity in hippocampus of pilocarpine-induced epileptic rats. Also, there was an increase in 5-HT(2C) gene expression and inositol triphosphate content in epileptic hippocampus. Carbamazepine and B. monnieri treatments reversed the alterations in 5-HT(2C) receptor binding, gene expression, and inositol triphosphate content in treated epileptic rats as compared to untreated epileptic rats. The forced swim test confirmed the depressive behavior pattern during epilepsy that was nearly completely reversed by B. monnieri treatment.
Krishnakumar
Amee
A
Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin 682022, Kerala, India.
Nandhu
M S
MS
Paulose
C S
CS
D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D034681:Bacopa / Q000737:chemistry*; D002220:Carbamazepine / Q000627:therapeutic use; D004195:Disease Models, Animal; D004827:Epilepsy / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D004873:Ergolines / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008297:Male; D010862:Pilocarpine; D028321:Plant Preparations / Q000627:therapeutic use*; D011485:Protein Binding / Q000187:drug effects; D011869:Radioligand Assay / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics / Q000378:metabolism*; D012702:Serotonin Antagonists / Q000378:metabolism; D018709:Statistics, Nonparametric; D013550:Swimming; D014316:Tritium / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
D000927:Anticonvulsants; D004873:Ergolines; D028321:Plant Preparations; D044405:Receptor, Serotonin, 5-HT2C; D012702:Serotonin Antagonists; D010862:Pilocarpine; D014316:Tritium; D002220:Carbamazepine; D015544:Inositol 1,4,5-Trisphosphate; C031649:mesulergine