title: Silver-coated gold nanoparticles as concentrating probes and matrices for surface-assisted laser desorption/ionization mass spectrometric analysis of aminoglycosides.
abstract: A novel method for the determination of aminoglycosides by surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) with the aid of silver-coated gold nanoparticles (Au@AgNPs) has been developed. The Au@AgNPs with surface capped by anionic citrate were used as concentrating probes as well as matrices in SALDI MS. Adsorption of aminoglycosides onto the nanoparticles was mainly through electrostatic attraction. The aminoglycoside-adsorbed nanoparticles were directly characterized by SALDI MS after a simple washing. Using Au@AgNPs to preconcentrate the aminoglycosides from 500 microL buffer solution, the limits of detection (LODs) at signal-to-noise ratio of 3 were 3, 25, 15, 30, and 38 nM for paromomycin, kanamycin A, neomycin, gentamicin, and apramycin, respectively. This method was successfully applied to the determination of aminoglycosides in human plasma samples. The LODs of aminoglycosides in plasma samples were 9, 130, 81, and 180 nM for paromomycin, kanamycin A, neomycin, and gentamicin, respectively. Recoveries of aminoglycosides in plasma samples were about 80%.
authors.affiliation: Department of Chemistry, Tunghai University, Taichung, Taiwan.
authors.forename: Ming-Ta
authors.initials: MT
authors.lastname: Wang
authors.forename: Ming-Han
authors.initials: MH
authors.lastname: Liu
authors.forename: C R Chris
authors.initials: CR
authors.lastname: Wang
authors.forename: Sarah Y
authors.initials: SY
authors.lastname: Chang
mesh_terms: D000327:Adsorption; D000617:Aminoglycosides / Q000097:blood / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000097:blood / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055672:Static Electricity
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012834:Silver; D006046:Gold
references.citation: Anal Chem. 2008 Sep 1;80(17):6758-63
references.pmid: 18681459
references.citation: J Phys Chem B. 2005 Jun 9;109(22):11135-8
references.pmid: 16852358
references.citation: J Am Soc Mass Spectrom. 2004 Nov;15(11):1629-35
references.pmid: 15519230
references.citation: Anal Chem. 2005 Sep 15;77(18):5912-9
references.pmid: 16159121
references.citation: Rapid Commun Mass Spectrom. 2008;22(3):283-90
references.pmid: 18186457
references.citation: Electrophoresis. 2006 Dec;27(23):4703-10
references.pmid: 17080474
references.citation: Histochemistry. 1981;71(1):1-16
references.pmid: 6785259
references.citation: Rapid Commun Mass Spectrom. 2007;21(13):2023-30
references.pmid: 17534856
references.citation: Eur J Cell Biol. 1985 Jul;38(1):87-93
references.pmid: 4029177
references.citation: Anal Chem. 2005 Jan 1;77(1):259-65
references.pmid: 15623304
references.citation: J Antimicrob Chemother. 2007 Feb;59(2):246-53
references.pmid: 17220162
references.citation: Anal Chem. 2004 Aug 1;76(15):4337-42
references.pmid: 15283570
references.citation: Anal Chem. 2006 Mar 1;78(5):1485-93
references.pmid: 16503598
references.citation: Anal Chem. 2007 Jul 1;79(13):4852-9
references.pmid: 17523592
references.citation: J Am Soc Mass Spectrom. 2007 May;18(5):910-8
references.pmid: 17368044
references.citation: J Chromatogr B Biomed Sci Appl. 2000 Sep 29;747(1-2):69-93
references.pmid: 11103900
references.citation: Histochemistry. 1985;82(4):321-9
references.pmid: 2409059
references.citation: J Chromatogr A. 2008 Mar 21;1185(1):93-101
references.pmid: 18262195
references.citation: J Am Chem Soc. 2005 Apr 20;127(15):5304-5
references.pmid: 15826152
references.citation: Curr Opin Chem Biol. 1999 Dec;3(6):694-704
references.pmid: 10600721
references.citation: Anal Chem. 2008 Sep 1;80(17):6796-9
references.pmid: 18671412
references.citation: Rapid Commun Mass Spectrom. 2004;18(20):2367-74
references.pmid: 15386634
references.citation: Anal Chem. 2007 Jan 15;79(2):434-44
references.pmid: 17222005
references.citation: Anal Chem. 2006 Aug 1;78(15):5491-6
references.pmid: 16878887
references.citation: Anal Chem. 2007 Feb 15;79(4):1626-33
references.pmid: 17297965
references.citation: J Chromatogr A. 2004 Nov 26;1058(1-2):197-201
references.pmid: 15595668
references.citation: Anal Bioanal Chem. 2006 Oct;386(3):699-704
references.pmid: 16685517