title: Significant differences in alkaloid content of Coptis chinensis (Huanglian), from its related American species.
abstract: BACKGROUND
  The growing popularity of Chinese herbal medicine in the United States has prompted large-scale import of raw herbs from Asia. Many of the Asian herbs have phylogenetically related North American species. We compared three phylogenetically related species, namely Coptis chinensis (Huanglian), Hydrastis canadensis and Coptis trifolia to show whether they can be substituted by one another in terms of alkaloid content.
  
  
  METHODS
  We used microwave assisted extraction to obtain alkaloids berberine, coptisine, palmatine and hydrastine. High performance liquid chromatography (HPLC) was used to quantify each alkaloid.
  
  
  RESULTS
  Hydrastis canadensis has the most berberine, whereas Coptis trifolia has the most coptisine. Hydrastine and palmatine were unique to Hydrastis canadensis and Coptis chinensis respectively.
  
  
  CONCLUSIONS
  Neither Hydrastis canadensis nor Coptis trifolia contains all the alkaloids found in Coptis chinensis used in Chinese medicine. Substitutes of this Chinese species by its American relatives are not recommended.
authors.affiliation: Department of Chemistry, St Lawrence University, Canton, New York 13617, USA. sakama04@stlawu.edu
authors.forename: Shreya
authors.initials: S
authors.lastname: Kamath
authors.forename: Matthew
authors.initials: M
authors.lastname: Skeels
authors.forename: Aswini
authors.initials: A
authors.lastname: Pai
references.citation: Evid Based Complement Alternat Med. 2005 Dec;2(4):465-73
references.pmid: 16322803
references.citation: Fitoterapia. 2001 Mar;72(3):221-9
references.pmid: 11295297
references.citation: Life Sci. 2006 Mar 27;78(18):2044-8
references.pmid: 16457853
references.citation: J Chromatogr A. 1998 Dec 31;829(1-2):137-41
references.pmid: 9923080
references.citation: J Pharm Pharmacol. 2002 Mar;54(3):435-9
references.pmid: 11902811
references.citation: Life Sci. 1997;60(23):PL345-51
references.pmid: 9180371
references.citation: Zhong Yao Cai. 2006 Jul;29(7):666-9
references.pmid: 17059001
references.citation: Biotechnol Bioeng. 1994 Jun 20;44(2):205-10
references.pmid: 18618686
references.citation: Immunol Lett. 1999 Jun 1;68(2-3):391-5
references.pmid: 10424448
references.citation: Clin Exp Pharmacol Physiol. 2004 Jan-Feb;31(1-2):65-9
references.pmid: 14756686
references.citation: J Agric Food Chem. 2003 Dec 3;51(25):7352-8
references.pmid: 14640583