title: Actinomyces timonensis sp. nov., isolated from a human clinical osteo-articular sample.
abstract: Gram-positive, non-spore-forming rods were isolated from a human osteo-articular sample (strain 7400942(T)). Based on cellular morphology and the results of biochemical analysis, this strain was tentatively identified as a novel species of the genus Actinomyces. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the bacterium was closely related to the type strain of Actinomyces denticolens (96.9 % 16S rRNA gene sequence similarity). A comparison of biochemical traits showed that strain 7400942(T) was distinct from A. denticolens in a number of characteristics, i.e. in contrast with A. denticolens, strain 7400942(T) was negative for nitrate reduction and for beta-galactosidase, alpha-glucosidase and alanine arylamidase activities, it was positive for acid production from N-acetylglucosamine, melezitose and glycogen, and it was negative for acid production from turanose. Matrix-assisted laser-desorption/ionization time-of-flight MS protein analysis confirmed that strain 7400942(T) represents a novel species, as scores obtained for its spectra were significant (>2.2) only with strain 7400942(T). On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain should be designated Actinomyces timonensis sp. nov.; the type strain is strain 7400942(T) (=CSUR P35(T)=CCUG 55928(T)).
authors.affiliation: Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, CNRS-IRD UMR 6236-198, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Renvoise
authors.affiliation: Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, CNRS-IRD UMR 6236-198, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, CNRS-IRD UMR 6236-198, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Roux
mesh_terms: D000190:Actinomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D000293:Adolescent; D001168:Arthritis / Q000382:microbiology*; D001483:Base Sequence; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012446:Sacroiliac Joint / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S