title: Effect of quercetin on platelet spreading on collagen and fibrinogen and on multiple platelet kinases.
abstract: alpha(2)beta(1) and alpha(IIb)beta(3) integrins, that support platelet adhesion to collagen and fibrinogen, respectively, share common signaling molecules. The effect of quercetin on platelet static adhesion to collagen and fibrinogen was assessed and correlated with its kinase inhibitory activity. Quercetin strongly abrogated PI3K and Src kinases, mildly inhibited Akt1/2, and slightly affected PKC, p38 and ERK1/2. Quercetin or the combined use of adenosine diphosphate and thromboxane A(2) inhibitors abrogated platelet spreading on these surfaces to a similar extent. We suggest that the inhibitory effect of quercetin on platelet kinases blocks early signaling events preventing a complete platelet spreading.
authors.affiliation: Unit of Haematology and Medical Oncology, Centro Regional de Hemodonación, University of Murcia, Spain. mleyrenn@um.es
authors.forename: Leyre
authors.initials: L
authors.lastname: Navarro-Núñez
authors.forename: María L
authors.initials: ML
authors.lastname: Lozano
authors.forename: Constantino
authors.initials: C
authors.lastname: Martínez
authors.forename: Vicente
authors.initials: V
authors.lastname: Vicente
authors.forename: José
authors.initials: J
authors.lastname: Rivera
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen; D005340:Fibrinogen; D006801:Humans; D016023:Integrins / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000187:drug effects*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013928:Thromboxane A2 / Q000037:antagonists & inhibitors
chemical_list: D016023:Integrins; D047428:Protein Kinase Inhibitors; D013928:Thromboxane A2; D000244:Adenosine Diphosphate; D005340:Fibrinogen; D003094:Collagen; D011794:Quercetin; D011494:Protein Kinases; D019869:Phosphatidylinositol 3-Kinases