Cytological analysis of meiosis in fixed Drosophila ovaries.
Methods are described to analyze two different parts of the Drosophila ovary, which correspond to early stages (pachytene) and late stages (metaphase I and beyond) of meiosis. In addition to taking into account morphology, the techniques differ by fixation conditions and the method to isolate the tissue. Most of these methods are whole mounts, which preserve the three-dimensional structure.
McKim
Kim S
KS
Waksman Institute and Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.
Joyce
Eric F
EF
Jang
Janet K
JK
D000818:Animals; D020732:Cytogenetic Analysis / Q000379:methods; D003584:Cytological Techniques / Q000379:methods*; D004330:Drosophila / Q000166:cytology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology; D005260:Female; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D010053:Ovary / Q000166:cytology* / Q000378:metabolism; D016707:Tissue Fixation / Q000379:methods