Fluorescence microscopy below the diffraction limit.
Fluorescence imaging with conventional microscopy has experienced numerous advances in almost every limiting factor from sensitivity to speed. But improved resolution beyond the fundamental limitation of light diffraction has been elusive until recent years. Now, techniques are available that surpass this barrier and improve resolution up to 10 times over that of conventional microscopy. This chapter provides an overview of these new "super-resolution" imaging methods.
Patterson
George H
GH
Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. pattersg@mail.nih.gov
D000818:Animals; D003584:Cytological Techniques; D015509:Developmental Biology; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods*
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