Labeling transplanted mice islet with polyvinylpyrrolidone coated superparamagnetic iron oxide nanoparticles for in vivo detection by magnetic resonance imaging.
Superparamagnetic iron oxide nanoparticles (SPIO) are emerging as a novel probe for noninvasive cell tracking with magnetic resonance imaging (MRI) and have potential wide usage in medical research. In this study, we have developed a method using high-temperature hydrolysis of chelate metal alkoxide complexes to synthesize polyvinylpyrrolidone coated iron oxide nanoparticles (PVP-SPIO), as a biocompatible magnetic agent that can efficiently label mice islet beta-cells. The size, crystal structure and magnetic properties of the as-synthesized nanoparticles have been characterized. The newly synthesized PVP-SPIO with high stability, crystallinity and saturation magnetization can be efficiently internalized into beta-cells, without affecting viability and function. The imaging of 100 PVP-SPIO-labeled mice islets in the syngeneic renal subcapsular model of transplantation under a clinical 3.0 T MR imager showed high spatial resolution in vivo. These results indicated the great potential application of the PVP-SPIO as an MRI contrast agent for monitoring transplanted islet grafts in the clinical management of diabetes in the near future.
Huang
Hai
H
Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR China.
Xie
Qiuping
Q
Kang
Muxing
M
Zhang
Bo
B
Zhang
Hui
H
Chen
Jin
J
Zhai
Chuanxin
C
Yang
Deren
D
Jiang
Biao
B
Wu
Yulian
Y
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D003287:Contrast Media / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D005434:Flow Cytometry; D007150:Immunohistochemistry; D050417:Insulin-Secreting Cells / Q000737:chemistry* / Q000166:cytology / Q000637:transplantation*; D007668:Kidney / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D011205:Povidone / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*
D001672:Biocompatible Materials; D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide; D011205:Povidone