Sequence-specific DNA detection by using biocatalyzed electrochemiluminescence and non-fouling surfaces.
We herein report a DNA sensor for the sequence-specific DNA detection by using glucose oxidase-based biocatalyzed electrochemiluminescence and non-fouling surfaces. In this design, a glucose oxidase-labeled sandwich-type DNA sensor was built on a non-fouling surfaces made of a mixed self-assembled monolayers (SAMs) incorporating thiolated oligonucleotides and oligo(ethylene glycol) (OEG) thiols (SH-DNA/OEG). The sequence-specific DNA sensing was accomplished by the electrochemiluminesce (ECL) signal of luminol with the in-situ generated H(2)O(2). The protein-resistant non-fouling surfaces significantly suppressed the non-specific adsorption of enzyme label on electrode and reduced the background noise of this sensor. This sensor was able to detect as little as 1 pM of target DNA in pure buffer matrix. In complicated biological fluids such as human serum, this non-fouling platform-based sensor also revealed superior performance over conventional sandwich-type DNA sensors with mercaptohexanol (MCH)-coated surfaces. This strategy combines the high sensitivity of enzymatic amplified ECL and protein-resistant non-fouling platform, which is expected to be extended to a range of biological detection.
Zhang
Lanyong
L
Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Li
Di
D
Meng
Weili
W
Huang
Qing
Q
Su
Yan
Y
Wang
Lihua
L
Song
Shiping
S
Fan
Chunhai
C
D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002384:Catalysis; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005949:Glucose Oxidase / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D013499:Surface Properties
D001672:Biocompatible Materials; D008165:Luminol; D004247:DNA; D005949:Glucose Oxidase