title: Ret-dependent cell rearrangements in the Wolffian duct epithelium initiate ureteric bud morphogenesis.
abstract: While the genetic control of renal branching morphogenesis has been extensively described, the cellular basis of this process remains obscure. GDNF/RET signaling is required for ureter and kidney development, and cells lacking Ret are excluded from the tips of the branching ureteric bud in chimeric kidneys. Here, we find that this exclusion results from earlier Ret-dependent cell rearrangements in the caudal Wolffian duct, which generate a specialized epithelial domain that later emerges as the tip of the primary ureteric bud. By juxtaposing cells with elevated or reduced RET activity, we find that Wolffian duct cells compete, based on RET signaling levels, to contribute to this domain. At the same time, the caudal Wolffian duct transiently converts from a simple to a pseudostratified epithelium, a process that does not require Ret. Thus, both Ret-dependent cell movements and Ret-independent changes in the Wolffian duct epithelium contribute to ureteric bud formation.
authors.affiliation: Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Chi
authors.forename: Odyssé
authors.initials: O
authors.lastname: Michos
authors.forename: Reena
authors.initials: R
authors.lastname: Shakya
authors.forename: Paul
authors.initials: P
authors.lastname: Riccio
authors.forename: Hideki
authors.initials: H
authors.lastname: Enomoto
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Licht
authors.forename: Naoya
authors.initials: N
authors.lastname: Asai
authors.forename: Masahide
authors.initials: M
authors.lastname: Takahashi
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Ohgami
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
authors.forename: Cathy
authors.initials: C
authors.lastname: Mendelsohn
authors.forename: Frank
authors.initials: F
authors.lastname: Costantini
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002678:Chimera / Q000033:anatomy & histology / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology; D004848:Epithelium / Q000033:anatomy & histology / Q000196:embryology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009024:Morphogenesis / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D019076:Transgenes; D014513:Ureter / Q000033:anatomy & histology / Q000196:embryology*; D014928:Wolffian Ducts / Q000033:anatomy & histology* / Q000196:embryology* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008164:Luminescent Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; C425468:Spry1 protein, mouse; D051096:Proto-Oncogene Proteins c-ret; C494999:Ret protein, mouse
references.citation: Dev Dyn. 2006 Feb;235(2):444-55
references.pmid: 16331648
references.citation: Anat Rec. 1978 Aug;191(4):447-58
references.pmid: 697056
references.citation: Development. 1997 Oct;124(20):4077-87
references.pmid: 9374404
references.citation: Nat Genet. 2005 Oct;37(10):1082-9
references.pmid: 16186816
references.citation: Genesis. 2009 Feb;47(2):61-6
references.pmid: 19111008
references.citation: Dev Biol. 2008 Sep 1;321(1):77-87
references.pmid: 18585375
references.citation: Dev Dyn. 2004 Feb;229(2):349-56
references.pmid: 14745960
references.citation: PLoS Genet. 2008 Dec;4(12):e1000316
references.pmid: 19112489
references.citation: Development. 2006 Aug;133(15):2995-3004
references.pmid: 16790474
references.citation: Nature. 2006 Jun 8;441(7094):746-9
references.pmid: 16760977
references.citation: Differentiation. 2006 Sep;74(7):402-21
references.pmid: 16916378
references.citation: BMC Dev Biol. 2001;1:4
references.pmid: 11299042
references.citation: Development. 1996 Jun;122(6):1919-29
references.pmid: 8674430
references.citation: Bioessays. 2006 Feb;28(2):117-27
references.pmid: 16435290
references.citation: Dev Dyn. 2005 Mar;232(3):685-94
references.pmid: 15712202
references.citation: Dev Cell. 2003 Jan;4(1):11-8
references.pmid: 12530959
references.citation: Dev Genet. 1999;24(3-4):241-51
references.pmid: 10322632
references.citation: Am J Hum Genet. 2008 Feb;82(2):344-51
references.pmid: 18252215
references.citation: Dev Biol. 2007 Feb 15;302(2):536-52
references.pmid: 17123506
references.citation: Dev Cell. 2005 Jan;8(1):65-74
references.pmid: 15621530
references.citation: Development. 2008 Jun;135(12):2055-64
references.pmid: 18480161
references.citation: Dev Biol. 2004 Jul 1;271(1):98-108
references.pmid: 15196953
references.citation: Dev Cell. 2008 Apr;14(4):570-81
references.pmid: 18410732
references.citation: Cytokine Growth Factor Rev. 2001 Dec;12(4):361-73
references.pmid: 11544105
references.citation: Nat Rev Mol Cell Biol. 2005 Aug;6(8):622-34
references.pmid: 16025097
references.citation: J Cell Sci. 2006 Aug 15;119(Pt 16):3376-84
references.pmid: 16882689
references.citation: Development. 2002 Nov;129(22):5301-12
references.pmid: 12399320
references.citation: Nat Rev Genet. 2007 Oct;8(10):791-802
references.pmid: 17878895
references.citation: Dev Biol. 2006 Feb 1;290(1):44-56
references.pmid: 16364283
references.citation: Bioessays. 2002 Oct;24(10):937-48
references.pmid: 12325126
references.citation: Dev Biol. 2004 Nov 1;275(1):44-67
references.pmid: 15464572
references.citation: J Anat. 2004 Apr;204(4):241-55
references.pmid: 15061751
references.citation: Annu Rev Cell Dev Biol. 2006;22:509-29
references.pmid: 16822174
references.citation: Dev Biol. 2006 Nov 15;299(2):466-77
references.pmid: 17022962
references.citation: Development. 2002 Nov;129(22):5151-60
references.pmid: 12399307
references.citation: Dev Cell. 2005 Dec;9(6):831-42
references.pmid: 16326394
references.citation: Dev Biol. 2008 Dec 1;324(1):88-98
references.pmid: 18835385
references.citation: Trends Cell Biol. 2005 Jan;15(1):34-42
references.pmid: 15653076
references.citation: Development. 2001 May;128(9):1573-85
references.pmid: 11290296
references.citation: J Cell Biol. 1998 Sep 7;142(5):1337-45
references.pmid: 9732293
references.citation: J Cell Biol. 2008 Jun 2;181(5):847-58
references.pmid: 18504303
references.citation: Development. 2004 Apr;131(7):1449-62
references.pmid: 15023929
references.citation: Differentiation. 2006 Sep;74(7):365-81
references.pmid: 16916375
references.citation: Curr Opin Genet Dev. 2007 Aug;17(4):281-6
references.pmid: 17624758
references.citation: Dev Cell. 2005 Feb;8(2):229-39
references.pmid: 15691764
references.citation: Genes Dev. 2001 Sep 15;15(18):2433-44
references.pmid: 11562352
references.citation: Development. 2003 Oct;130(19):4527-37
references.pmid: 12925581
references.citation: Nature. 1994 Jan 27;367(6461):380-3
references.pmid: 8114940