title: Probing single enzyme kinetics in real-time.
abstract: Studies of reaction kinetics at the single enzyme level have become possible due to the swift development of single molecule detection techniques. In this tutorial review, which targets both general and expert research chemists in the biochemical or physical (organic) chemistry field, we briefly introduce some of these fluorescence microscopic techniques and discuss selected studies on single molecule enzymology. The nature of the frequently observed fluctuations in the reaction rate, the so-called dynamic disorder, is described based on various available models.
authors.affiliation: Department of Materials Science and Technology of Polymers, University of Twente, MESA+ Institute for Nanotechnology, PO Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Ramon
authors.initials: R
authors.lastname: Groote
authors.forename: Holger
authors.initials: H
authors.lastname: Schönherr
authors.forename: G Julius
authors.initials: GJ
authors.lastname: Vancso
mesh_terms: D004798:Enzymes; D007700:Kinetics; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological
chemical_list: D004798:Enzymes