Extended-spectrum and CMY-type beta-lactamase-producing Escherichia coli in clinical samples and retail meat from Pittsburgh, USA and Seville, Spain.
Infections due to Escherichia coli producing extended-spectrum beta-lactamase (ESBL) or CMY-type beta-lactamase (CMY) are increasingly observed in non-hospitalized patients. The origin of these organisms is uncertain, but retail meat contaminated with E. coli may be a source. In the present study, clinical information and strains collected from patients infected or colonized with ESBL-producing and CMY-producing E. coli at hospitals in Pittsburgh, USA and Seville, Spain were investigated. Retail meat purchased in these cities was also studied for the presence of these organisms. Twenty-five and 79 clinical cases with ESBL-producing E. coli and 22 cases and one case with CMY-producing E. coli were identified in Pittsburgh and Seville, respectively. Among them all, community-acquired and healthcare-associated cases together constituted 60% of the cases in Pittsburgh and 73% in Seville. Community-acquired cases were more common in Seville than in Pittsburgh (49% vs. 13%; p <0.001). ESBL-producing and CMY-producing E. coli isolates were commonly recovered from the local retail meat. In particular, 67% (8/12) of retail chickens in Seville and 85% (17/20) of those in Pittsburgh contained ESBL-producing and CMY-producing E. coli isolates, respectively. Among the ESBL-producing isolates, CTX-M and SHV were the most common ESBL types in both clinical and meat isolates. Approximately half of the ESBL-producing and CMY-producing E. coli isolates from meat belonged to phylogenetic groups associated with virulent extra-intestinal infections in humans. Community and healthcare environments are now significant reservoirs of ESBL-producing and CMY-producing E. coli. Retail meat is a potential source of these organisms.
Doi
Y
Y
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.
Paterson
D L
DL
Egea
P
P
Pascual
A
A
López-Cerero
L
L
Navarro
M D
MD
Adams-Haduch
J M
JM
Qureshi
Z A
ZA
Sidjabat
H E
HE
Rodríguez-Baño
J
J
D000818:Animals; D002417:Cattle; D016009:Chi-Square Distribution; D002645:Chickens; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology*; D017720:Molecular Epidemiology; D010414:Pennsylvania / Q000453:epidemiology; D011446:Prospective Studies; D013030:Spain / Q000453:epidemiology; D013552:Swine; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics
D001618:beta-Lactamases