PKC differentially translocates during spaced and massed training in Aplysia.
Learning is highly regulated by the pattern of training. In Aplysia, an important organism for the development of cellular and molecular models of learning, spaced versus massed application of the same stimulus leads to different forms of memory. A critical molecular step underlying memory is the serotonin (5HT)-mediated activation of the novel PKC Apl II. Here, we demonstrate that activation of PKC Apl II is highly sensitive to the pattern of 5HT application. Spaced applications downregulate PKC translocation through PKA signaling, whereas massed applications lead to persistent translocation of PKC. Differential regulation of PKC translocation is mediated by competing feedback mechanisms that act through protein synthesis. These studies elucidate a fundamental molecular difference between spaced and massed training protocols.
Farah
Carole A
CA
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
Weatherill
Daniel
D
Dunn
Tyler W
TW
Sossin
Wayne S
WS
D000818:Animals; D001048:Aplysia / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D007858:Learning / Q000187:drug effects / Q000502:physiology*; D008568:Memory / Q000187:drug effects / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000201:enzymology; D012701:Serotonin / Q000494:pharmacology
D047428:Protein Kinase Inhibitors; D012701:Serotonin; D011493:Protein Kinase C
Science. 1993 Apr 9;260(5105):222-6
7682336
Neuron. 1998 Dec;21(6):1423-34
9883734
J Neurosci. 2001 Feb 15;21(4):1247-56
11160395
Neuron. 2000 Apr;26(1):219-31
10798406
Neuron. 2001 Jul 19;31(1):143-54
11498057
Biol Bull. 2006 Jun;210(3):174-91
16801493
Mol Cell Biol. 2008 Aug;28(15):4719-33
18505819
Learn Mem. 2007 Apr 02;14(4):236-46
17404386
Science. 2009 Jun 19;324(5934):1536-40
19443737
Science. 1997 Feb 28;275(5304):1318-20
9036859
Learn Mem. 2002 Jan-Feb;9(1):29-40
11917004
Neurochem Res. 1990 Apr;15(4):431-4
2201927
J Neurosci. 1982 Dec;2(12):1673-81
6292379
J Neurochem. 2004 Oct;91(1):210-9
15379901
Neuron. 1993 May;10(5):921-9
7684236
Curr Biol. 2008 Jun 24;18(12):920-5
18571411
Learn Mem. 1997 Jan-Feb;3(5):389-401
10456106
J Neurosci. 1982 Dec;2(12):1682-91
6292380
Traffic. 2007 May;8(5):462-70
17376169
Cell. 1994 Oct 7;79(1):35-47
7923375
Prog Brain Res. 2008;169:3-25
18394465
Neuron. 1999 Jan;22(1):147-56
10027297
J Neurosci. 2009 Apr 22;29(16):5100-7
19386905
J Neurophysiol. 2006 Apr;95(4):2713-20
16236785
Biochem Biophys Res Commun. 2007 Aug 3;359(3):563-7
17548057
J Neurosci. 2006 Jan 18;26(3):1026-35
16421322
Learn Mem. 1998 Jul-Aug;5(3):246-56
10454368
J Neurosci. 2006 Aug 23;26(34):8847-56
16928874
J Neurochem. 2000 Aug;75(2):872-81
10899966
J Neurosci. 2002 Jul 1;22(13):5412-22
12097493
Mol Cell Biol. 2006 Nov;26(22):8586-98
16982686
Science. 2001 Nov 2;294(5544):1030-8
11691980
Science. 2004 May 14;304(5673):1024-7
15143285
J Neurosci. 1996 Jan 15;16(2):425-35
8551327
J Neurochem. 1998 Feb;70(2):572-83
9453551
Prog Brain Res. 2008;169:277-92
18394481
J Neurosci. 1997 Oct 1;17(19):7237-44
9295370
J Neurophysiol. 2004 Oct;92(4):2468-86
15140903