[HPLC-MS analysis of quercetin and its metabolites in portal vein after intragastrical administration of quercetin in rats].
OBJECTIVE
To analyse the plasma contents of querceitn and its metabolites in portal vein in rats.


METHODS
Male Wistar rats were orally administrated with quercetin at the dose of 40 mg/kg. The blood samples were corrected from the portal vein 1 h later. The quercetin and its metabolites in plasma were analysed with high performance liquid chromatography-mass spetrometry (HPLC-MS) method.


RESULTS
The quercetin, quercetin-3-glucuronide, quercetin glucuronide sulfate, 3'-O-methylated quercetin, 4'-O- methylated quercetin and quercetin sulfate were detected in portal vein.


CONCLUSIONS
The results indicated that the quercetin was extensively metabolized in intestine and entered the portal vein in various forms after administration.
An
Daizhi
D
Department of Nutrition, Institute of Hygiene and Environmental Medicine, Tianjin 300050, China.
Wu
Shengming
S
Wei
Jingyu
J
Yang
Jijun
J
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D011169:Portal Vein / Q000378:metabolism; D011794:Quercetin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; C443401:quercetin 3-O-glucuronide; C047368:3-methylquercetin; D011794:Quercetin