In vivo analysis of the Notch receptor S1 cleavage.
A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.
Lake
Robert J
RJ
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America.
Grimm
Lisa M
LM
Veraksa
Alexey
A
Banos
Andrew
A
Artavanis-Tsakonas
Spyros
S
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D004330:Drosophila; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D021381:Protein Transport; D051880:Receptors, Notch / Q000737:chemistry / Q000378:metabolism*
D051880:Receptors, Notch
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