title: Tracheal suture in rats with hypothyroidism: wound healing study.
abstract: OBJECTIVE
  To evaluate the influence of hypothyroidism in tracheal wound healing process.
  
  
  METHODS
  A controlled study was designed with 48 male Wistar rats, divided into two groups: study and control groups. In the first one hypothyroidism was surgically induced and 10 weeks after, a tracheal opening followed by suture was performed in both groups, subdivided into 7, 14, and 21 days in accordance with the date of animals death. A laboratorial evaluation was performed to prove the decreased in thyroid function in the study group. Also a macroscopic evaluation through a stablished protocol and a microscopic analysis with Hematoxylin-eosin and Sirius-Red staining methods were done.
  
  
  RESULTS
  The laboratorial evaluation certified suppressed thyroid function in the study group. The macroscopic evaluation showed the presence of suture blockade in the study group in all the evaluated days. Microscopic analysis showed a prolongated inflammatory process and less collagen with delay in organization in the study group comparing to control group. All these data were statistic significant.
  
  
  CONCLUSIONS
  Hypothyroidism had an influence in tracheal wound healing process, promoting delay in the inflammatory and organization processes and diminished tissue collagen quantity.
authors.affiliation: Principles of Surgery Post-Graduate Program, Evangelical Medical School, Curitiba, PR, Brazil. ipem@evangelico.org.br
authors.forename: Elise
authors.initials: E
authors.lastname: Zimmermann
authors.forename: Jurandir Marcondes
authors.initials: JM
authors.lastname: Ribas-Filho
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Malafaia
authors.forename: Carmen Austrália Paredes Marcondes
authors.initials: CA
authors.lastname: Ribas
authors.forename: Paulo Afonso Nunes
authors.initials: PA
authors.lastname: Nassif
authors.forename: Edmar
authors.initials: E
authors.lastname: Stieven Filho
authors.forename: Paulo Eduardo
authors.initials: PE
authors.lastname: Przysiezny
mesh_terms: D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D004396:Coloring Agents; D004195:Disease Models, Animal; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D006416:Hematoxylin; D007037:Hypothyroidism / Q000503:physiopathology*; D007249:Inflammation / Q000473:pathology; D008297:Male; D008853:Microscopy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014132:Trachea / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D003094:Collagen; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin