Electrophoretically mediated microanalysis technique as a tool for the rapid screening of novel acetylcholinesterase inhibitors.
An electrophoretically mediated microanalysis technique was developed for the rapid screening of acetylcholinesterase inhibitors. Activity of four original compounds, N-benzylpiperidine, carbamoyloxyphenyl derivatives and reference inhibitor--tacrine was determined and compared with the data obtained from Ellman's assay. Percentage of enzyme inhibition detected at inhibitor concentration of 3.33.10(-4) M was between 0 and 78% and was the highest for tacrine. The comparison of data obtained from the EMMA assay and Ellman's test proved inhibitory activity of novel compounds.
Bajda
Marek
M
Jagiellonian University Medical College, Department of Physicochemical Drug Analysis, Chair of Pharmaceutical Chemistry, Medyczna 9, 30-688 Kraków, Poland.
Wieckowska
Anna
A
Malawska
Barbara
B
D002800:Cholinesterase Inhibitors / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration
D002800:Cholinesterase Inhibitors