Motility and protein phosphorylation in healthy and asthenozoospermic sperm.
The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.
Chan
Chying-Chyuan
CC
Graduate Institute of Medical Sciences, National Defence Medical Center, Taipei, Taiwan, ROC.
Shui
Hao-Ai
HA
Wu
Ching-Hsiang
CH
Wang
Chih-Yuan
CY
Sun
Guang-Huan
GH
Chen
Han-Min
HM
Wu
Gwo-Jang
GJ
D000328:Adult; D000818:Animals; D053627:Asthenozoospermia / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010766:Phosphorylation; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D055815:Young Adult
D014404:Tubulin