title: Expression of the surface antigens of lymphocytes and the levels of cytokines in mice infected with Aspergillus fumigatus.
abstract: BACKGROUND
  Aspergillus fumigatus (A. fumigatus) is an opportunistic fungus that causes invasive aspergillosis. Determining the immune changes during A. fumigatus infection and the factors leading to such changes clearly will make it possible to prevent the spread of the infection and to provide new strategies in the treatment of infection. Thus, the present study aims at determining the changes of lymphocyte surface antigens which develop during A. fumigatus infection and the role of cytokines in immune response.
  
  
  METHODS
  The expression of the surface antigens of lymphocytes was analysed by flow cytometry and the cytokine levels were determined by ELISA in a mouse model of aspergillosis.
  
  
  RESULTS
  It was observed that in mice infected by A. fumigatus the percentage of CD19+ B cells and the levels of IL-4 and IL-10 increased when compared to those in noninfected mice cells.
  
  
  CONCLUSIONS
  These results suggest that Th2 type cytokines are important in the pathogenesis of A. fumigatus infection. Humoral immunity is considered to be effective during A. fumigatus infection because of the increase in Th2 type response.
authors.affiliation: Gazi University, Faculty of Medicine, Department of Medical Microbiology, Turkey. isilfidan@yahoo.com
authors.forename: Isil
authors.initials: I
authors.lastname: Fidan
authors.forename: Ayse
authors.initials: A
authors.lastname: Kalkanci
authors.forename: Sezen
authors.initials: S
authors.lastname: Bolat
authors.forename: Emine
authors.initials: E
authors.lastname: Yesilyurt
authors.forename: Berna
authors.initials: B
authors.lastname: Erdal
authors.forename: Sultan
authors.initials: S
authors.lastname: Yolbakan
authors.forename: Semra
authors.initials: S
authors.lastname: Kustimur
authors.forename: Turgut
authors.initials: T
authors.lastname: Imir
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology*; D001228:Aspergillosis / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D018941:Antigens, CD19; D016753:Interleukin-10; D015847:Interleukin-4