title: Estimation of atrazine-degrading genetic potential and activity in three French agricultural soils.
abstract: The impact of organic amendment (sewage sludge or waste water) used to fertilize agricultural soils was estimated on the atrazine-degrading activity, the atrazine-degrading genetic potential and the bacterial community structure of soils continuously cropped with corn. Long-term application of organic amendment did not modify atrazine-mineralizing activity, which was found to essentially depend on the soil type. It also did not modify atrazine-degrading genetic potential estimated by quantitative PCR targeting atzA, B and C genes, which was shown to depend on soil type. The structure of soil bacterial community determined by RISA fingerprinting was significantly affected by organic amendment. These results showed that modification of the structure of soil bacterial community in response to organic amendment is not necessarily accompanied by a modification of atrazine-degrading genetic potential or activity. In addition, these results revealed that different soils showing similar atrazine-degrading genetic potentials may exhibit different atrazine-degrading activities.
authors.affiliation: INRA-CMSE, UMR INRA-Université de Bourgogne, Dijon, France. fmartin@dijon.inra.fr
authors.forename: Fabrice
authors.initials: F
authors.lastname: Martin-Laurent
authors.forename: Laurent
authors.initials: L
authors.lastname: Cornet
authors.forename: Lionel
authors.initials: L
authors.lastname: Ranjard
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: López-Gutiérrez
authors.forename: Laurent
authors.initials: L
authors.lastname: Philippot
authors.forename: Christophe
authors.initials: C
authors.lastname: Schwartz
authors.forename: Rémi
authors.initials: R
authors.lastname: Chaussod
authors.forename: Gérard
authors.initials: G
authors.lastname: Catroux
authors.forename: Guy
authors.initials: G
authors.lastname: Soulas
mesh_terms: D001280:Atrazine / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D044822:Biodiversity; D001711:Biotransformation; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D005602:France; D016133:Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology; D003313:Zea mays / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001280:Atrazine