title: Partial protective responses induced by a recombinant cysteine proteinase from Leishmania (Leishmania) amazonensis in a murine model of cutaneous leishmaniasis.
abstract: A 500 bp fragment encoding an isoform of cysteine proteinase from Leishmania (Leishmania) amazonensis was subcloned and expressed in the pHis vector, resulting in a recombinant protein of 24 kDa, rLacys24. In Western blots of L. (L.) amazonensis extracts, antibodies directed to rLacys24 recognized a cysteine proteinase isoform of 30 kDa. Analysis by fluorescence-activated cell sorter showed a significantly higher expression of CD8(+) lymphocytes in animals immunized with rLacys24 plus CFA, whereas a low expression of CD4(+) lymphocytes was observed in these animals. The cytotoxicity of lymphocytes isolated from mice immunized with rLacys24 plus CFA on L. (L.) amazonensis-infected macrophages was significantly higher than that observed in the presence of lymphocytes from control animals. Immunization of BALB/c mice with rLacys24 plus CFA resulted in a low but significant decrease of foot lesions after challenge with L. (L.) amazonensis compared to those exhibited by control mice.
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Carlos Eduardo Cardoso
authors.initials: CE
authors.lastname: Fedeli
authors.forename: Josie Haydée Lima
authors.initials: JH
authors.lastname: Ferreira
authors.forename: Juliana Sekeres
authors.initials: JS
authors.lastname: Mussalem
authors.forename: Ieda Maria
authors.initials: IM
authors.lastname: Longo-Maugéri
authors.forename: Luciana Girotto
authors.initials: LG
authors.lastname: Gentil
authors.forename: Márcia Regina Machado
authors.initials: MR
authors.lastname: dos Santos
authors.forename: Simone
authors.initials: S
authors.lastname: Katz
authors.forename: Clara Lúcia
authors.initials: CL
authors.lastname: Barbiéri
mesh_terms: D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D057056:Cysteine Proteases / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007894:Leishmania mexicana / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000517:prevention & control*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D016054:DNA, Protozoan; D011994:Recombinant Proteins; D057056:Cysteine Proteases