Conferin, potent antioxidant and anti-inflammatory  isoflavone from Caragana conferta Benth.
Conferin (1), a new isoflavone, has been isolated from the ethyl acetate soluble fraction of Caragana conferta Benth. along with seven known compounds, namely biochanin A (2), p-hydroxybenzoic acid (3), 3,5-dimethoxybenzoic acid (4), ursolic acid (5), erythrodiol (6), pinoresinol (7), and syringresinol (8), reported for the first time from this species. The structure of the new isoflavone was deduced on the basis of spectroscopic studies. Compounds 1 and 2 were investigated for biological activities and showed significant anti-inflammatory activity in carrageenan induced paw edema of rats. Evaluation of antioxidant activity by the radical scavenging method indicated that compound 1 is a potent antioxidant while 2 is moderately active. It was also shown that the reducing capability of compound 2 was remarkably increased in a concentration dependent manner as compared to 1. Compound 1 showed moderate inhibitory activity against the enzyme lipoxygenase, while 2 showed weak activity.
Khan
Amna Nisar
AN
International Center for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.
Perveen
Shagufta
S
Malik
Abdul
A
Afza
Nighat
N
Iqbal
Lubna
L
Latif
Mehreen
M
Saleem
Muhammad
M
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D032602:Caragana / Q000737:chemistry*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D016166:Free Radical Scavengers; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008084:Lipoxygenase / Q000187:drug effects; D016859:Lipoxygenase Inhibitors; D015394:Molecular Structure; D051381:Rats; D013057:Spectrum Analysis
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D007529:Isoflavones; D016859:Lipoxygenase Inhibitors; D008084:Lipoxygenase