title: Selective molecularly imprinted stationary phases for bisphenol A analysis prepared by modified precipitation polymerization.
abstract: Bisphenol A (BPA)-imprinted polymeric microspheres were synthesized by modified precipitation polymerization (MPP) method. Influences of cross-linker, monomer, porogen volume, and agitation on polymerization were investigated. Proper amount of cross-linker ethyleneglycol-dimethacrylate (EGDMA) was critical to achieve narrowly dispersed microspheres. For template BPA, monomer 4-vinylpyridine (4-VP) was better than MAA to get the best imprinted effects. The optimum template/monomer ratio was 1:6. Increasing porogen volume increased size dispersity and decreased binding characters. Agitation increased coagulation and resulted in irregular particles. Microspheres with the best binding characters were used as selective stationary phase of chromatographic column to detect BPA in milk, pig urine, and chicken meat. Under optimal chromatographic conditions, the calibration graph was linear with R2 = 0.9994 in the range of 3-50 micromol/L. The LOD and LOQ were 1 and 3 micromol/L, respectively. When large amounts (20 mL or 20 g) of samples were analyzed, the recoveries ranged from 70.2 to 87.3% with RSD less than 4.85% in all samples spiked with 0.05-0.2 micromol/L BPA. The intra-day and inter-day RSD were less than 1.83 and 3.96%, respectively. Microspheres prepared by MPP are successfully used in molecularly imprinted polymer (MIP)-based analytical column to detect trace BPA in different biologic samples with acceptable accuracy and repeatability.
authors.affiliation: School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.forename: Ren-li
authors.initials: RL
authors.lastname: Zhang
authors.forename: Chang-hua
authors.initials: CH
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D001826:Body Fluids / Q000737:chemistry; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D010316:Particle Size; D010636:Phenols / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D003432:Cross-Linking Reagents; D010636:Phenols; D011108:Polymers; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds