Beijing genotype and other predominant Mycobacterium tuberculosis spoligotypes observed in Mashhad city, Iran.
OBJECTIVE
The purpose of this study was to understand the molecular epidemiology of tuberculosis in Khorasan province of Iran was studied by spoligotyping 113 Mycobacterium tuberculosis isolates. The spoligotyping results were in comparison to the word Spoligotyping Database of Institute Pasteur de Guadeloupe (SpolDB4). Spoligotyping data from Iran has rarely been described and there is limited information on the major circulating clades of M. tuberculosis in Iran.


METHODS
Spoligotyping was performed on 113 M. tuberculosis isolates from Mashhad patients between November 2004 and September 2005.


RESULTS
The study found 57 spoligopatterns. 17 clusters and 32 true orphan genotype. The biggest cluster with 13 isolates had not been previously reported. The Beijing genotype was seen in eight (7.1%) isolates.


CONCLUSIONS
Genotyping and Spoligotyping gives a unifying framework for both epidemiology and evolutionary analysis of M. tuberculosis populations.
Rohani
M
M
Department of Microbiology and Immunology , Kashan University of Medical Sciences, P.O. Box: 87155-111, Iran. kia_rohani@yahoo.com
Farnia
P
P
Nasab
M Naderi
MN
Moniri
R
R
Torfeh
M
M
Amiri
M M
MM
D000328:Adult; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*
D004269:DNA, Bacterial