Comparison of in vivo and in vitro survival and fecundity rates of the poultry red mite, Dermanyssus gallinae.
To assist in the testing of possible antigens in developing a vaccine against the poultry red mite (Dermanyssus gallinae De Geer), a rapid and reliable in vitro screening method is critical. This short paper describes how D. gallinae survival and fecundity rates in an in vivo feeding device compared to that of mites fed using an in vitro method. Results showed that survival of fed D. gallinae females and mites overall was greater in vitro, although there was no difference between male survival and fecundity between in vivo and in vitro designs. The in vitro feeding device described therefore has the potential to provide reliable results, comparable to those obtained by in vivo testing, to allow for the rapid screening of D. gallinae antigens.
Arkle
S
S
School of Agriculture, Food and Rural Development, Newcastle University, Newcastle Upon Tyne, NE1 7RU England, UK.
George
D R
DR
Guy
J H
JH
Sparagano
O A E
OA
D000818:Animals; D005260:Female; D005298:Fertility / Q000502:physiology; D008136:Longevity / Q000502:physiology*; D008297:Male; D008925:Mites / Q000502:physiology*; D012098:Reproduction