OPLA scaffold, collagen I, and horse serum induce an higher degree of myogenic differentiation of adult rat cardiac stem cells.
In the last few years, a major goal of cardiac research has been to drive stem cell differentiation to replace damaged myocardium. Several research groups have attempted to differentiate potential cardiac stem cells (CSCs) using bi- or three-dimensional systems supplemented with growth factors or molecules acting as differentiating substances. We hypothesize that these systems failed to induce a complete differentiation because they lacked an architectural space. In the present study, we isolated a pool of small proliferating and fibroblast-like cells from adult rat myocardium. The phenotype of these cells was assessed and the characterized cells were cultured in a collagen I/OPLA scaffold with horse serum to obtain fine myocardial differentiation. C-Kit(POS)/Sca-1(POS) CSCs fully differentiated in vitro when an environment more similar to the CSC niche was created. These experiments demonstrated an important model for the study of the biology of CSCs and the biochemical pathways that lead to myocardial differentiation. The results pave the way for a new surgical approach.
Di Felice
Valentina
V
Department of Experimental Medicine, Human Anatomy Section, University of Palermo, Palermo, Italy. vdfelice@inwind.it
Ardizzone
Nella Maria
NM
De Luca
Angela
A
Marcianò
Vito
V
Marino Gammazza
Antonella
A
Macaluso
Filippo
F
Manente
Lucrezia
L
Cappello
Francesco
F
De Luca
Antonio
A
Zummo
Giovanni
G
D000199:Actins / Q000378:metabolism; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002469:Cell Separation / Q000379:methods; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D024042:Collagen Type I / Q000494:pharmacology*; D018031:Connexin 43 / Q000378:metabolism; D005260:Female; D050991:GATA4 Transcription Factor / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D006736:Horses; D007381:Intermediate Filament Proteins / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D046529:Microscopy, Electron, Transmission; D024510:Muscle Development / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D054457:Tissue Scaffolds; D014157:Transcription Factors; D020107:Troponin T / Q000378:metabolism
D000199:Actins; D024042:Collagen Type I; D018031:Connexin 43; D050991:GATA4 Transcription Factor; D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D060850:LIM-Homeodomain Proteins; D018995:Myosin Heavy Chains; D009419:Nerve Tissue Proteins; D064231:Nestin; D019009:Proto-Oncogene Proteins c-kit; D014157:Transcription Factors; D020107:Troponin T; C577484:NES protein, human; C577486:Nes protein, rat; C063843:insulin gene enhancer binding protein Isl-1; C498862:Acta2 protein, rat