title: Celeribacter neptunius gen. nov., sp. nov., a new member of the class Alphaproteobacteria.
abstract: A whitish Gram-negative, motile, aerobic bacterium, designated strain H 14(T), was isolated from seawater collected at St Kilda beach in Port Phillip Bay, Melbourne, Australia. Analysis of 16S rRNA gene sequences revealed that the organism belonged to the Roseobacter lineage of the class Alphaproteobacteria, forming a distinct evolutionary lineage at the genus level. Strain H 14(T) was distantly related to the genera Nautella, Ruegeria and Pseudoruegeria (family Rhodobacteraceae). Strain H 14(T) was unable to degrade gelatin, casein, chitin, agar and starch, did not produce any carotenoids, did not possess bacteriochlorophyll a and had a limited ability to utilize carbon sources. Strain H 14(T) grew with concentrations of 1-8 % (w/v) NaCl and over a temperature range of 5-35 degrees C. Phosphatidylglycerol was the major phospholipid (90 %); phosphatidylcholine (7.9 %) and phosphatidylethanolamine (2.0 %) were present in minor quantities. The predominant fatty acids were C(18 : 1)omega7c (82.4 %), C(18 : 1)omega9c (5.1 %) and C(18 : 0) (3.8 %). The DNA G+C composition for strain H 14(T) was 59.1 mol%. Based on the results of physiological, biochemical, chemotaxonomic and phylogenetic investigations, a new genus, Celeribacter gen. nov., with the type species Celeribacter neptunius sp. nov. is proposed. The type strain of the type species is H 14(T) (=KMM 6012(T)=CIP 109922(T)).
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.
authors.forename: Hayden
authors.initials: H
authors.lastname: Webb
authors.affiliation: Université de Nice Sophia-Antipolis and CNRS UMR 6543, Virtual Biology Laboratory, Centre de Biochimie, Parc Valrose, F06108, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Christen
authors.affiliation: Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041 Vladivostok, Russian Federation.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
authors.forename: Valeriya V
authors.initials: VV
authors.lastname: Kurilenko
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
authors.forename: Nataliya I
authors.initials: NI
authors.lastname: Kalinovskaya
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Crawford
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001483:Base Sequence; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S