Application of separated leaf cell suspension to xenobiotic metabolism in plant.
Metabolic profiles of (14)C-labeled primary metabolites from several pesticides, 4-cyanophenol (1), 3-phenoxybenzoic acid (2), 3-phenoxybenzyl alcohol (3), 3,5-dichloroaniline (4), and (1RS)-trans-2,2-dimethyl-3-(2-methylprop-1-enyl)cyclopropanecarboxylic acid (5), were examined by using enzymatically separated leaf cell suspension from seedlings of cabbage ( Brassica oleracea ) and tomato ( Lycopersicon esculentum ). After 1 day of incubation, the metabolites were extensively transformed in cabbage, whereas they were scarcely metabolized in tomato. The major metabolic pathways were the phase II reactions leading to a number of conjugates such as glucoside/malonylglucoside of 1-5, malate of 2, and glutamate of 4. The oxidation of 1 and 2 was observed as a minor reaction to produce 4-hydroxybezoic acid and 3-(4-hydroxyphenoxy)benzoic acid. The chemical identities of the secondary metabolites were determined by various spectrometric analyses (LC-MS, LC-MS/MS, and NMR) and/or HPLC cochromatography with the synthetic reference standards. As a result, this separated leaf cell suspension system was found to well reproduce the in vivo plant metabolism.
Fujisawa
Takuo
T
Environmental Health Science Laboratory, Sumitomo Chemical Company, Ltd., 4-2-1 Takatsukasa, Takarazuka, Hyogo 665-8555, Japan. fujisawat1@sc.sumitomo-chem.co.jp
Matoba
Yoshihide
Y
Katagi
Toshiyuki
T
D001937:Brassica / Q000737:chemistry* / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000378:metabolism*; D055442:Metabolome; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism
D002247:Carbon Isotopes