[Introduction to the standard operation program of spoligotyping on mycobacterium tuberculosis in China].
OBJECTIVE
To study and preliminarily evaluate the standard spacer oligonucleotide typing (Spoligotyping) method and the application on Mycobacterium tuberculosis (M. tuberculosis).


METHODS
Spoligotyping on 224 M. tuberculosis strains was studied by the molecular biological techniques, including DNA isolation, PCR, reverse line blot hybridization, together with data analysis software BioNumerics (Version 5.0).


RESULTS
Standardization on both Spoligotyping method and parameters in result analysis and the usage of the analysis software were studied. Through this method, 224 M. tuberculosis clinical strains were classified into 2 clusters including 129 Beijing family strains and 95 non-Beijing family strains. The predominant strains belonged to Beijing family.


CONCLUSIONS
Standard Spoligotyping method was preliminary determined in China, showing that it was a simple, rapid, and robust method for simultaneous detection and typing of M. tuberculosis. This method can be used for tracing the source of infection and understanding the epidemic trend of M. tuberculosis. Spoligotyping can also be served as a method for simultaneous detection and typing of M. tuberculosis, and to identify Beijing family strains.
Dong
Hai-yan
HY
State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Lu
Bing
B
Zhang
Yuan-yuan
YY
Liu
Zhi-guang
ZG
Zhao
Xiu-qin
XQ
Jiang
Yi
Y
Wan
Kang-lin
KL
D015373:Bacterial Typing Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015345:Oligonucleotide Probes
D015345:Oligonucleotide Probes