Differences in genetic instability and cellular phenotype among Barrett's, cardiac, and gastric intestinal metaplasia in a Japanese population with Helicobacter pylori.
OBJECTIVE
Intestinal metaplasia is considered to be a precursor lesion in both Barrett's and intestinal-type gastric cancer. The aim was to clarify the differences in molecular pathology between specialized intestinal metaplasia (SIM) in Barrett's oesophagus (BO), cardiac (CIM) and gastric intestinal metaplasia (GIM).


RESULTS
Eighty-eight SIM cases with BO, 30 CIM cases and 52 GIM cases in patients with or without Helicobacter pylori infectionwere analysed for genetic instability and Das-1. Microsatellite instability and a loss of heterozygosity were evaluated at five microsatellite loci. The incidence of genetic instability was 55.7% in SIM, 40.0% in CIM and 23.1% in GIM, revealing a significant difference between SIM and GIM (P < 0.0005). For each microsatellite marker analysed, there were obvious differences in frequency among the three conditions. Das-1 reactivity was significantly higher in SIM than in CIM or GIM (P < 0.0001, both). Interestingly, both genetic instability and Das-1 reactivity in SIM showed a significantly higher incidence in patients with H. pylori infection than in those without (P < 0.005 and P < 0.01, respectively).


CONCLUSIONS
SIM is distinct from CIM and GIM, and the pathogenesis of SIM, like that of GIM, is associated to some degree with H. pylori infection in a Japanese population.
Watari
Jiro
J
Department of Gastroenterology, Kushiro Medical Association Hospital, Kushiro, Japan. watarij@hyo-med.ac.jp
Moriichi
Kentaro
K
Tanabe
Hiroki
H
Sato
Ryu
R
Fujiya
Mikihiro
M
Miwa
Hiroto
H
Das
Kiron M
KM
Kohgo
Yutaka
Y
D000368:Aged; D001471:Barrett Esophagus / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D002299:Cardia / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005748:Gastric Fundus / Q000473:pathology*; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics* / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D007564:Japan; D019656:Loss of Heterozygosity; D008297:Male; D008679:Metaplasia; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm