A vertebrate Polycomb response element governs segmentation of the posterior hindbrain.
Chromatin remodeling by Polycomb group (PcG) and trithorax group (trxG) proteins regulates gene expression in all metazoans. Two major complexes, Polycomb repressive complexes 1 and 2 (PRC1 and PRC2), are thought to mediate PcG-dependent repression in flies and mammals. In Drosophila, PcG/trxG protein complexes are recruited by PcG/trxG response elements (PREs). However, it has been unclear how PcG/trxG are recruited in vertebrates. Here we have identified a vertebrate PRE, PRE-kr, that regulates expression of the mouse MafB/Kreisler gene. PRE-kr recruits PcG proteins in flies and mouse F9 cells and represses gene expression in a PcG/trxG-dependent manner. PRC1 and 2 bind to a minimal PRE-kr region, which can recruit stable PRC1 binding but only weak PRC2 binding when introduced ectopically, suggesting that PRC1 and 2 have different binding requirements. Thus, we provide evidence that similar to invertebrates, PREs act as entry sites for PcG/trxG chromatin remodeling in vertebrates.
Sing
Angela
A
Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, ON, M5G 1X5, Canada.
Pannell
Dylan
D
Karaiskakis
Angelo
A
Sturgeon
Kendra
K
Djabali
Malek
M
Ellis
James
J
Lipshitz
Howard D
HD
Cordes
Sabine P
SP
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D002645:Chickens; D042002:Chromatin Assembly and Disassembly; D007446:Chromosome Inversion; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D051258:MafB Transcription Factor / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D063150:Polycomb Repressive Complex 1; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements; D012249:Rhombencephalon / Q000378:metabolism*
D029721:Drosophila Proteins; D051258:MafB Transcription Factor; C490406:Mafb protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C490239:Nnat protein, mouse; C066770:Pc protein, Drosophila; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1