title: Reduced formation of advanced glycation endproducts via interactions between glutathione peroxidase 3 and dihydroxyacetone kinase 1.
abstract: Dihydroxyacetone (DHA) induces the formation of advanced glycation endproducts (AGEs), which are involved in several diseases. Earlier, we identified dihydroxyacetone kinase 1 (Dak1) as a candidate glutathione peroxidase 3 (Gpx3)-interacting protein in Saccharomyces cerevisiae. This finding is noteworthy, as no clear evidence on the involvement of oxidative stress systems in DHA-induced AGE formation has been found to date. Here, we demonstrate that Gpx3 interacts with Dak1, alleviates DHA-mediated stress by upregulating Dak activity, and consequently suppresses AGE formation. Based on these results, we propose that defense systems against oxidative stress and DHA-induced AGE formation are related via interactions between Gpx3 and Dak1.
authors.affiliation: Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Hana
authors.initials: H
authors.lastname: Lee
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Chi
authors.forename: Phil Young
authors.initials: PY
authors.lastname: Lee
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Kang
authors.forename: Sayeon
authors.initials: S
authors.lastname: Cho
authors.forename: Chong-Kil
authors.initials: CK
authors.lastname: Lee
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
mesh_terms: D004098:Dihydroxyacetone / Q000378:metabolism*; D017353:Gene Deletion; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000096:biosynthesis* / Q000235:genetics; D018384:Oxidative Stress; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017127:Glycation End Products, Advanced; D029701:Saccharomyces cerevisiae Proteins; D005979:Glutathione Peroxidase; C494438:HYR1 protein, S cerevisiae; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase; D004098:Dihydroxyacetone