Molecular characterization of the Salicornia brachiata SbMAPKK gene and its expression by abiotic stress.
MAPK cascade is an important intracellular signaling module and function as a convergent point for crosstalk during abiotic stress signaling. In this study SbMAPKK gene has been isolated from Salicornia brachiata, a highly salt tolerant plant growing in costal marshes of Gujarat, India. The SbMAPKK gene is 1,023 bp long, encodes a 340 amino acid protein with an estimated molecular mass of 37.4 kDa. The SbMAPKK shows high sequence identity with NbMKK1 from N. benthamiana, LeMKK4 from Lycopersicon esculentum. SbMAPKK constitutes 11 conserved subdomains of protein kinase. Northern analysis revealed that SbMAPKK transcript expression is induced by different stresses like dehydration, cold and salt, however, maximum expression is observed during cold stress. The phylogenetic analysis and genomic organization confirms that it is an intron less gene belonging 'D' group in MAPKK family.
Agarwal
Pradeep K
PK
Discipline of Marine Biotechnology and Ecology, Central Salt, and Marine Chemicals Research Institute (Council of Scientific and Industrial Research), Bhavnagar, Gujarat 364 002, India. pagarwal@csmcri.org
Gupta
Kapil
K
Jha
Bhavanath
B
D000595:Amino Acid Sequence; D019663:Chenopodiaceae / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000302:isolation & purification; D003681:Dehydration / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D055049:Salt Tolerance / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological / Q000235:genetics*
D018076:DNA, Complementary; D020929:Mitogen-Activated Protein Kinase Kinases