title: Dynamic change of Adamalysin 19 (ADAM19) in human placentas and its effects on cell invasion and adhesion in human trophoblastic cells.
abstract: Human ADAM19 is a recently identified member of the ADAM family. It is highly expressed in human placentas, but its dynamic change and function at the human feto-maternal interface during placentation remain to be elucidated. In this present study, the spatial and temporal expression and cellular localization of ADAM19 in normal human placentas were first demonstrated, and the effects of ADAM19 on trophoblast cell adhesion and invasion were further investigated by using a human choriocarcinoma cell line (JEG-3) as an in vitro model. The data demonstrated that ADAM19 was widely distributed in villous cytotrophoblast cells, syncytiotrophoblast cells, column trophoblasts, and villous capillary endothelial cells during early pregnancy. The mRNA and protein level of ADAM19 in placentas was high at gestational weeks 8-9, but diminished significantly at mid- and term pregnancy. In JEG-3 cells, the overexpression of ADAM19 led to diminished cell invasion, as well as increases in cell adhesiveness and the expression of E-cadherin, with no changes in beta-catenin expression observed. These data indicate that ADAM19 may participate in the coordinated regulation of human trophoblast cell behaviors during the process of placentation.
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: MeiRong
authors.initials: M
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.forename: YuXia
authors.initials: Y
authors.lastname: Li
authors.forename: QingXiang Amy
authors.initials: QA
authors.lastname: Sang
authors.forename: YanLing
authors.initials: Y
authors.lastname: Wang
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002822:Choriocarcinoma; D002824:Chorionic Villi / Q000502:physiology; D017931:DNA Primers; D036861:Delivery, Obstetric; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007231:Infant, Newborn; D010920:Placenta / Q000502:physiology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014327:Trophoblasts / Q000187:drug effects / Q000502:physiology*; D014594:Uterine Neoplasms
chemical_list: D017931:DNA Primers; D051722:ADAM Proteins; C411902:ADAM19 protein, human