title: Viremia and antibody response in green monkeys (Chlorocebus aethiops sabaeus) infected with dengue virus type 2: a potential model for vaccine testing.
abstract: The increasingly limited availability and high cost of the hitherto most commonly used monkey species in dengue vaccine research has augmented the importance of identifying alternative suitable models for these studies. In this study we examined the capacity of green monkeys (Chlorocebus aethiops sabaeus) to develop dengue viremia, and thus provide a potential model for dengue vaccine testing. Monkeys were inoculated with two different doses of dengue virus type 2. All animals in both groups became viremic after inoculation of the virus. In the lower dose group, mean viremia duration of 5.66 days was detected, whereas in the group that received the 106 PFU dose, viremia had a mean duration of only 1.66 days. Antibody titers were similar to those obtained in previous experiments with rhesus and cynomolgus macaques. We conclude that green monkeys develop viremia and antibody responses and therefore provide a potential model for the preclinical evaluation of novel candidates for dengue vaccines.
authors.affiliation: Center for Genetic Engineering and Biotechnology, Ave 31, P.O. Box 6162, Havana 6, 10600, Cuba. jorge.martin@cigb.edu.cu
authors.forename: Jorge
authors.initials: J
authors.lastname: Martín
authors.forename: Lisset
authors.initials: L
authors.lastname: Hermida
authors.forename: Jorge
authors.initials: J
authors.lastname: Castro
authors.forename: Laura
authors.initials: L
authors.lastname: Lazo
authors.forename: Rafael
authors.initials: R
authors.lastname: Martínez
authors.forename: Lázaro
authors.initials: L
authors.lastname: Gil
authors.forename: Yaremis
authors.initials: Y
authors.lastname: Romero
authors.forename: Pedro
authors.initials: P
authors.lastname: Puente
authors.forename: Santiago
authors.initials: S
authors.lastname: Zaragoza
authors.forename: Karelia
authors.initials: K
authors.lastname: Cosme
authors.forename: Maria G
authors.initials: MG
authors.lastname: Guzmán
authors.forename: Jane
authors.initials: J
authors.lastname: Cardosa
authors.forename: Gerardo
authors.initials: G
authors.lastname: Guillén
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops / Q000821:virology*; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D003716:Dengue Virus / Q000276:immunology* / Q000472:pathogenicity*; D004195:Disease Models, Animal; D014612:Vaccines / Q000276:immunology; D014766:Viremia
chemical_list: D000914:Antibodies, Viral; D014612:Vaccines