title: Homology modelling and spectroscopy, a never-ending love story.
abstract: Homology modelling is normally the technique of choice when experimental structure data are not available but three-dimensional coordinates are needed, for example, to aid with detailed interpretation of results of spectroscopic studies. Herein, the state of the art of homology modelling will be described in the light of a series of recent developments, and an overview will be given of the problems and opportunities encountered in this field. The major topic, the accuracy and precision of homology models, will be discussed extensively due to its influence on the reliability of conclusions drawn from the combination of homology models and spectroscopic data. Three real-world examples will illustrate how both homology modelling and spectroscopy can be beneficial for (bio)medical research.
authors.affiliation: Centre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre, 6500 HB Nijmegen, The Netherlands. hvensela@cmbi.ru.nl
authors.forename: Hanka
authors.initials: H
authors.lastname: Venselaar
authors.forename: Robbie P
authors.initials: RP
authors.lastname: Joosten
authors.forename: Bas
authors.initials: B
authors.lastname: Vroling
authors.forename: Coos A B
authors.initials: CA
authors.lastname: Baakman
authors.forename: Maarten L
authors.initials: ML
authors.lastname: Hekkelman
authors.forename: Elmar
authors.initials: E
authors.lastname: Krieger
authors.forename: Gert
authors.initials: G
authors.lastname: Vriend
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D017385:Sequence Homology; D013057:Spectrum Analysis / Q000379:methods*; D013113:Spin Labels
chemical_list: D011506:Proteins; D013113:Spin Labels