Determination of cyanide, in urine and gastric content, by electrospray ionization tandem mass spectrometry after direct flow injection of dicyanogold.
A rapid and sensitive electrospray ionization tandem mass spectrometric (ESI-MS-MS) procedure was developed for the determination of cyanide (CN(-)). CN(-) in biological fluids was reacted with NaAuCl(4) to produce dicyanogold, Au(CN)(2)(-), which was extracted with methyl isobutyl ketone (MIBK). One microliter of the extract was injected directly into an ESI-MS-MS instrument. Quantification of CN(-) was performed by selected reaction monitoring of the product ion CN(-) at m/z 26 that derived from precursor ion Au(CN)(2)(-) at m/z 249. CN(-) could be measured in the quantification range of 10(-7) to 5x10(-5) M with the limit of detection at 4x10(-8) M using 10 microL of urine within 10 min. A victim's urine and gastric content were diluted with water to 4-fold and 500-fold and measured, respectively.
Minakata
Kayoko
K
Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan. kminakat@hama-med.ac.jp
Nozawa
Hideki
H
Gonmori
Kunio
K
Suzuki
Masako
M
Suzuki
Osamu
O
D003485:Cyanates / Q000737:chemistry*; D003486:Cyanides / Q000032:analysis* / Q000652:urine; D017022:Flow Injection Analysis / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008742:Methyl n-Butyl Ketone / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013270:Stomach / Q000737:chemistry
D003485:Cyanates; D003486:Cyanides; C043041:gold cyanide; D008742:Methyl n-Butyl Ketone; D006046:Gold; C005458:methyl isobutyl ketone