Effect of Ccapsaicin on tNOX (ENOX2) protein expression in stomach cancer cells.
Tumor-associated NADH oxidase (tNOX, also known as ENOX2) is a growth-related protein expressed in transformed cells. Previous reports have revealed that the inhibition of tNOX activity by the anti-cancer drug, capsaicin, correlates with a reduction in growth of cancer cells, indicating a close relationship between tNOX activity and cell growth. Moreover, the study of depleted tNOX expression by RNA interference in HeLa cells suggests that it may be associated with the ability of tumor cells to acquire an aggressive phenotype, particularly in relation to cell proliferation. A key role for tNOX in regulating cell growth is further supported by the observation that the growth rate of MEF cells from tNOX-overexpressing transgenic mice is approximately two-fold greater than that of wild-type cells. The purpose of this study was to investigate the anti-proliferative effect of capsaicin on tNOX expression level in stomach cancer cells. We showed that capsaicin induced cytotoxicity in SCM cells concomitantly with apoptosis, PARP cleavage, and down-regulation of tNOX protein.
Wang
Hsi-Ming
HM
Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.
Chueh
Pin Ju
PJ
Chang
Sheng-Pang
SP
Yang
Chi-Lien
CL
Shao
Kuo-Ning
KN
D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002211:Capsaicin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018689:Sensory System Agents / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000378:metabolism*
D018689:Sensory System Agents; D009247:NADH, NADPH Oxidoreductases; C431909:tumor-associated NADH oxidase; D002211:Capsaicin