Angiotensin-I converting enzyme inhibitory activity of hydrolysates from oat (Avena sativa) proteins by in silico and in vitro analyses.
The potential for producing antihypertensive peptides from oat proteins through enzymatic hydrolysis was assessed in silico and confirmed in vitro. Thermolysin (EC 3.4.24.27) was predicted using BIOPEP database as the enzyme that would theoretically produce the most angiotensin I converting enzyme (ACE) inhibitory peptides from oat. Experimental evidence confirmed that strong ACE-inhibitory activity was produced under various hydrolysis conditions. Hydrolysates produced under high enzyme-to-substrate ratio (3%) short time (20 min) (HEST) and low enzyme-to-substrate ratio (0.1%) long time (120 min) (LELT) conditions had IC(50) values of 30 and 50 microg/mL, respectively. After simulated gastrointestinal digestion, the IC(50) of the HEST hydrolysate was 35 microg/mL whereas the IC(50) of the LELT hydrolysate was higher at 85 microg/mL. Ultrafiltration revealed that potent ACE-inhibitory peptides had molecular weights below 3 kDa. This study demonstrates the usefulness of in silico analysis to select enzymes for hydrolysis of proteins not previously examined as sources of bioactive peptides.
Cheung
Imelda W Y
IW
Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, Canada.
Nakayama
Satoko
S
Hsu
Monica N K
MN
Samaranayaka
Anusha G P
AG
Li-Chan
Eunice C Y
EC
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D018554:Avena / Q000737:chemistry*; D004063:Digestion; D006868:Hydrolysis; D066298:In Vitro Techniques; D010194:Pancreatin / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D013820:Thermolysin / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D010446:Peptide Fragments; D010940:Plant Proteins; D010194:Pancreatin; D010434:Pepsin A; D013820:Thermolysin