title: Voltammetric and spectroscopic investigations on the mechanism of interaction of buzepide methiodide with protein.
abstract: Adsorption or immobilization of proteins on solid surfaces promotes the biological responses to materials. Using immobilization technique, we have prepared human serum albumin (HSA) modified glassy carbon electrode (GCE) and employed it to probe the mode of interaction between antidepressant drug, buzepide methiodide (BZP) and HSA. At HSA modified GCE, the peak potential of BZP appeared at more positive potential compared to that at bare electrode thereby indicating the hydrophobic mode of interaction between BZP and HSA. Peak currents of BZP decreased upon the addition of HSA at bare GCE with positive shift in peak potential. Further, no new peaks were observed in presence of HSA. From electrochemical data, the binding constant and binding ratio between HSA and BZP were calculated to be 9.33 x 10(6)M(-1) and 1:2, respectively. FT-IR and circular dichroism (CD) studies revealed that the secondary structure of protein was perturbed upon interaction with BZP.
authors.affiliation: Department of Chemistry, Karnatak University, Dharwad 580003, India.
authors.forename: Shankara S
authors.initials: SS
authors.lastname: Kalanur
authors.forename: Jaldappagari
authors.initials: J
authors.lastname: Seetharamappa
authors.forename: Umesha
authors.initials: U
authors.lastname: Katrahalli
mesh_terms: D001381:Azepines / Q000378:metabolism*; D002244:Carbon / Q000737:chemistry; D002942:Circular Dichroism; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006801:Humans; D012709:Serum Albumin / Q000378:metabolism*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001381:Azepines; D012709:Serum Albumin; D012996:Solutions; C021334:buzepide metiodide; D002244:Carbon