title: Ontogeny of ATP hydrolysis and isoform expression of the plasma membrane Ca(2+)-ATPase in mouse brain.
abstract: BACKGROUND
  Plasma membrane Ca2+-ATPases (PMCAs) are high affinity Ca2+ transporters actively involved in intracellular Ca2+ homeostasis. Considering the critical role of Ca2+ signalling in neuronal development and plasticity, we have analyzed PMCA-mediated Ca2+-ATPase activity and PMCA-isoform content in membranes from mouse cortex, hippocampus and cerebellum during postnatal development.
  
  
  RESULTS
  PMCA activity was detected from birth, with a faster evolution in cortex than in hippocampus and cerebellum. Western blots revealed the presence of the four isoforms in all regions, with similar increase in their expression patterns as those seen for the activity profile. Immunohistochemistry assays in cortex and hippocampus showed co-expression of all isoforms in the neuropil associated with synapses and in the plasma membrane of pyramidal cells soma, while cerebellum showed a more isoform-specific distribution pattern in Purkinje cells.
  
  
  CONCLUSIONS
  These results show an upregulation of PMCA activity and PMCA isoforms expression during brain development in mouse, with specific localizations mainly in cerebellum. Overall, our findings support a close relationship between the ontogeny of PMCA isoforms and specific requirements of Ca2+ during development of different brain areas.
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain. danielmc@unex.es
authors.forename: Daniel
authors.initials: D
authors.lastname: Marcos
authors.forename: M Rosario
authors.initials: MR
authors.lastname: Sepulveda
authors.forename: María
authors.initials: M
authors.lastname: Berrocal
authors.forename: Ana M
authors.initials: AM
authors.lastname: Mata
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002531:Cerebellum / Q000201:enzymology* / Q000254:growth & development; D002540:Cerebral Cortex / Q000201:enzymology* / Q000254:growth & development; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000201:enzymology* / Q000254:growth & development; D006868:Hydrolysis; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000201:enzymology; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D013569:Synapses / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium