Organic-inorganic hybrid material for the cells immobilization: long-term viability mechanism and application in BOD sensors.
In this paper, organic-inorganic hybrid material, which is composed of silica and the grafting copolymer of poly (vinyl alcohol) and 4-vinylpyridine (PVA-g-P(4-VP)), was employed to immobilize Trichosporon cutaneum strain 2.570 cells. Cells entrapped into the hybrid material were found to keep a long-term viability. The mechanism of such a long-term viability was investigated by using confocal laser scanning microscopy (CLSM). Our studies revealed that arthroconidia produced in the extracellular material might play an important role in keeping the long-term viability of the immobilized microorganism. After the arthroconidia were activated, an electrochemical biochemical oxygen demand (BOD) sensor based on cell/hybrid material-modified supporting membrane was constructed for verifying the proposed mechanism. The results and insight gained from the present experiments can be widely used to various biosensor designs.
Liu
Ling
L
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renming Street 5625, Changchun 130022, Jilin, PR China.
Shang
Li
L
Guo
Shaojun
S
Li
Dan
D
Liu
Changyu
C
Qi
Li
L
Dong
Shaojun
S
D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002470:Cell Survival; D018914:Cells, Immobilized; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010100:Oxygen / Q000032:analysis*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D014159:Transducers; D014250:Trichosporon / Q000502:physiology*
D011725:Pyridines; D011142:Polyvinyl Alcohol; C029351:4-vinylpyridine; D010100:Oxygen