title: Effect of a new titanium coating material (CaTiO3-aC) prepared by thermal decomposition method on osteoblastic cell response.
abstract: Titanium and hydroxyapatite (HA) are widely used as biomaterials for dental and medical applications. HA-coated titanium implants have excellent biocompatibility and mechanical properties. However, the adherence of HA film formed on titanium substrate is weak because of the lack of chemical interaction between HA and titanium. A solution to this problem is to form an intermediate film on titanium substrate, which provide excellent adherence to both titanium substrate and HA. We developed a novel biomaterial called calcium titanate-amorphous carbon (CaTiO(3)-aC) coating prepared by modified thermal decomposition method. The purpose of this study was to evaluate the effect of CaTiO(3)-aC and HA coating (positive control), and Ti (negative control) on osteoblastic (MT3T3-E1) cell responses. An increased cellular proliferation was observed in CaTiO(3)-aC coating compared to HA coating. The maximum expressions of ALP activity, Col I and ALP mRNA were higher and achieved in shorter period of time in CaTiO(3)-aC coating compared to others. These results demonstrated that CaTiO(3)-aC promoted better cell attachment, cellular proliferation, and osteoblastic differentiation compared with HA. In conclusion, we suggested that CaTiO(3)-aC could be considered as an important candidate as a coating material.
authors.affiliation: Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8525, Japan. mi8@md.okayama-u.ac.jp
authors.forename: Miho
authors.initials: M
authors.lastname: Inoue
authors.forename: Andrea P
authors.initials: AP
authors.lastname: Rodriguez
authors.forename: Tohru
authors.initials: T
authors.lastname: Takagi
authors.forename: Naoki
authors.initials: N
authors.lastname: Katase
authors.forename: Midori
authors.initials: M
authors.lastname: Kubota
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Masahisa
authors.initials: M
authors.lastname: Inoue
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagaoka
authors.forename: Shin
authors.initials: S
authors.lastname: Takagi
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018786:Bone Substitutes; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006358:Hot Temperature; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010006:Osteoblasts / Q000166:cytology*; D011208:Powders; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D011208:Powders; D014025:Titanium