title: [Expression of ICOSL on human coronary artery endothelial cells and its being interferentialed by ox-LDL].
abstract: OBJECTIVE
  To study the expression of inducible co-stimulator ligand(ICOSL) on human coronary artery endothelial cells(HCAEC) and its being interferentialed by oxidized low density lipoprotein(ox-LDL).
  
  
  METHODS
  ICOSL expression levels were determined by the fluorescence, reverse transcription PCR (RT-PCR) and Western blot, respectively.
  
  
  RESULTS
  The ICOSL mRNA OD values of control and 100 mg/L ox-LDL group was 0.071+/-0.035 and 0.186+/-0.044, respectively. The Western blot A values of control and 100 mg/L ox-LDL group was 10.88+/-1.53 and 16.03+/-4.08, respectively. ox-LDL increased expression of ICOSL mRNA and protein(P<0.05).
  
  
  CONCLUSIONS
  ICOSL can express on the HCAEC. ox-LDL can up-regulate the expression of ICOSL.
authors.affiliation: Department of Cardiology, Guangzhou General Hospital of Guangzhou Military Direct, Guangzhou 510010, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Li
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Zhu
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Chang-Jiang
authors.initials: CJ
authors.lastname: Hong
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: He
authors.forename: Ding-Cheng
authors.initials: DC
authors.lastname: Xiang
authors.forename: Jian
authors.initials: J
authors.lastname: Qiu
mesh_terms: D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand; D008077:Lipoproteins, LDL / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; C498514:ICOSLG protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein