Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation.
Mucins are high molecular mass glycoproteins with oligosaccharides O-bonded to the protein core. beta-elimination is the most popular method used for releasing of O-glycans. However to such released glycoforms it is difficult to introduce a label to amplify a signal for oligosaccharide detection.In our study we used a combination of the beta-elimination and hydrazinolysis methods. Released glycoforms were labeled with para-amino benzooic acid ethyl ester (ABBE) and fractionated on HPLC column.This combined procedure seems to be a good tool for O-glycans analysis.
Kisiel
D G
DG
Department of Medical Chemistry, Medical University of Białystok Mickiewicza, Białystok, Poland.
Radziejewska
I
I
Gindzieński
A
A