title: A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat.
abstract: The effect of drought and salinity stress on the seedlings of the somatic hybrid wheat cv. Shanrong No. 3 (SR3) and its parent bread wheat cv. Jinan 177 (JN177) was investigated using two-dimensional gel electrophoresis and mass spectrometry. Of a set of 93 (root) and 65 (leaf) differentially expressed proteins (DEPs), 34 (root) and six (leaf) DEPs were cultivar-specific. The remaining DEPs were salinity/drought stress-responsive but not cultivar-specific. Many of the DEPs were expressed under both drought and salinity stresses. The amounts of stress-responsive DEPs between SR3 and JN177 were almost equivalent, whereas only some of these DEPs were shared by the two cultivars. Overall, the number of salinity-responsive DEPs was greater than the number of drought-responsive DEPs. And most of the drought-responsive DEPs also responded to salinity. There are both similarities and differences in the responses of wheat to salinity and drought. A parallel transcriptomics analysis showed that the correlation between transcriptional and translational patterns of DEPs was poor. The enhanced drought/salinity tolerance of SR3 appears to be governed by a superior capacity for osmotic and ionic homeostasis, a more efficient removal of toxic by-products, and ultimately a better potential for growth recovery.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, China.
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Peng
authors.forename: Mengcheng
authors.initials: M
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Hongjun
authors.initials: H
authors.lastname: Lv
authors.forename: Cuiling
authors.initials: C
authors.lastname: Li
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
mesh_terms: D001939:Bread; D055864:Droughts; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D007178:Inbreeding; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054712:Salinity; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013312:Stress, Physiological; D014908:Triticum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome