Underlying mechanism of penehyclidine hydrochloride on isolated rat uterus.
We investigated the underlying mechanisms of penehyclidine hydrochloride (PHC), a novel selective anticholinergic drug on isolated rat uterus. The method of radio-immunity assay was further employed to determine cyclic adenosine mono phosphate (cAMP) levels in isolated rat uterus for comparing with selective effect on muscarinic receptor subtypes. In the assay, PHC could decrease the content of cAMP in isolated rat uterus, but the difference was not statistically significant at dose of 10 mumol/L. In conclusion, our results suggested that PHC has no or poor effect on M(2) receptor subtypes in isolated rat uterus.
Xiao
Hong-tao
HT
Department of Pharmacy, Sichuan Academy of Medical Sciences & Sichuan Provincial Peoples Hospital, 610072 Chengdu, China. xht927@163.com
Liao
Zhi
Z
Meng
Xian-min
XM
Yan
Xiao-yan
XY
Chen
Shu-jie
SJ
Mo
Zheng-ji
ZJ
D000818:Animals; D018680:Cholinergic Antagonists / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D011812:Quinuclidines / Q000494:pharmacology*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D043585:Receptor, Muscarinic M2 / Q000187:drug effects / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism
D018680:Cholinergic Antagonists; D011812:Quinuclidines; D043585:Receptor, Muscarinic M2; D011976:Receptors, Muscarinic; C486140:penehyclidine; D000242:Cyclic AMP