Fungal diversity in composting process of pig manure and mushroom cultural waste based on partial sequence of large subunit rRNA.
Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU) ribosomal RNA (rRNA) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; Geotrichum sp., Debaryomyces hansenii, Monographella nivalis, Acremonium strictum, Acremonium alternatum, Cladosporium sphaerospermum, Myriangium durosai, Pleurotus eryngii, Malassezia globosa, Malassezia restricta, Rhodotorula glutinis, and Fusarium sporotrichioides. Geotrichum sp. of the class Saccharomycetes was the most predominant fungal species throughout the composting process (185 out of a total of 236 identified clones, or 78.4%), followed by Acremonium strictum (7.6%), Monographella nivalis (5.1%), and Pleurotus eryngii (3.8%). The prevalence of Geotrichum sp. was the lowest (61.1%) at the beginning of composting, and then gradually increased to 92.5% after 10 days of composting.
Cho
Kye Man
KM
Research Institute of Agricultural Science, Sunchon National University, Sunchon, Republic of Korea.
Kwon
Eun Ju
EJ
Kim
Sung Kyum
SK
Kambiranda
Devaiah M
DM
Math
Reukaradhya K
RK
Lee
Young Han
YH
Kim
Jungho
J
Yun
Han Dae
HD
Kim
Hoon
H
D000363:Agaricales / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001673:Biodegradation, Environmental; D044822:Biodiversity; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D055165:Debaryomyces / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008372:Manure / Q000382:microbiology*; D003904:Mitosporic Fungi / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013552:Swine
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D008372:Manure