Identification of specific microRNAs responding to low and high dose gamma-irradiation in the human lymphoblast line IM9.
MicroRNAs (miRNAs) are short single-stranded RNA molecules that regulate the stability or translational efficiency of target messenger RNAs. Specific miRNAs are required for strict tissue- and developmental stage-specific expression. These miRNAs have roles in many human tumor malignancies and their expression is specifically regulated on each stage of oncogenic process. Therefore, miRNA expression profiling can be used as a new class of biomarker that indicates the development of cancer. Many recent studies indicated that cell exposure to ionizing radiation also induces various physiological responses including DNA repair, cell cycle arrest, cell death and differentiation. In addition, some studies suggest that exposure to low dose radiation induces a favorable effect on cells. However, the functions of miRNAs related to the response of irradiated cells have not been well studied, especially after low dose radiation. In this study, expression profiles of miRNAs isolated from irradiated cells at low and high dose radiation were analyzed with microarrays, and these data were validated using quantitative RT-PCR. Here, we describe specific miRNAs that are expressed in a dose-dependent manner that serve as new markers of irradiated immune cells.
Cha
Hwa Jun
HJ
Functional Genoproteome Research Centre, Konkuk University, Seoul 143-701, Korea.
Seong
Ki Moon
KM
Bae
Seunghee
S
Jung
Jin Hyuk
JH
Kim
Cha Soon
CS
Yang
Kwang-Hee
KH
Jin
Young-Woo
YW
An
Sungkwan
S
D001402:B-Lymphocytes / Q000528:radiation effects*; D015153:Blotting, Western; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000528:radiation effects*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D035683:MicroRNAs