Chromatographic methods for determination of S-substituted cysteine derivatives--a comparative study.
A novel HPLC method for determination of a wide variety of S-substituted cysteine derivatives in Allium species has been developed and validated. This method allows simultaneous separation and quantification of S-alk(en)ylcysteine S-oxides, gamma-glutamyl-S-alk(en)ylcysteines and gamma-glutamyl-S-alk(en)ylcysteine S-oxides in a single run. The procedure is based on extraction of these amino acids and dipeptides by methanol, their derivatization by dansyl chloride and subsequent separation by reversed phase HPLC. The main advantages of the new method are simplicity, excellent stability of derivatives, high sensitivity, specificity and the ability to simultaneously analyze the whole range of S-substituted cysteine derivatives. This method was critically compared with other chromatographic procedures used for quantification of S-substituted cysteine derivatives, namely with two other HPLC methods (derivatization by o-phthaldialdehyde/tert-butylthiol and fluorenylmethyl chloroformate), and with determination by gas chromatography or capillary electrophoresis. Major advantages and drawbacks of these analytical procedures are discussed. Employing these various chromatographic methods, the content and relative proportions of individual S-substituted cysteine derivatives were determined in four most frequently consumed alliaceous vegetables (garlic, onion, shallot, and leek).
Kubec
Roman
R
Department of Applied Chemistry, University of South Bohemia, Branisovská 31, 37005 Ceské Budejovice, Czech Republic. kubecr@centrum.cz
Dadáková
Eva
E
D000490:Allium / Q000737:chemistry*; D053000:Analytic Sample Preparation Methods; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D003619:Dansyl Compounds; D019075:Electrophoresis, Capillary; D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry*
D003619:Dansyl Compounds; C495663:S-propyl cysteine sulfoxide; C406690:ethiin; C008389:methiin; C006453:alliin; D003545:Cysteine; C029038:dansyl chloride