Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet.
A study was undertaken to investigate the effect of administering praziquantel (PZQ), focusing on the liver stereological findings of malnourished mice infected with Schistosoma mansoni. Thirty female Swiss Webster mice (age: 21 days; weight: 8-14 g) were fed either a low-protein diet (8%) or standard chow (22% protein) for 15 days. Five mice in each group were infected with 50 cercariae each of the BH strain (Brazil). PZQ therapy (80 mg/kg body weight, per day) was started on the 50th day of infection and consisted of daily administration for 5 days. Volume density (hepatocytes, sinusoids and hepatic fibrosis) was determined by stereology using a light microscope. Body weight gain and total serum albumin levels were always lower in undernourished mice. Our stereological study demonstrated that treatment increased both volume density of hepatocytes in mice fed standard chow (47.56%, treated group and 12.06%, control) and low-protein chow (30.98%, treated group and 21.44%, control), and hepatic sinusoids [standard chow (12.52%, treated group and 9.06%, control), low-protein chow (14.42%, treated group and 8.46%, control)], while hepatic fibrosis was reduced [standard chow (39.92%, treated group and 78.88%, control) and low-protein chow (54.60%, treated group and 70.10%, control)]. On the other hand, mice fed low-protein chow decreased density volume of hepatocytes and hepatic fibrosis. In conclusion, our findings indicate that treatment with PZQ ameliorates hepatic schistosomiasis pathology even in mice fed a low-protein diet.
Barros
L A
LA
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, RJ, Brasil.
Costa-Silva
M
M
Biolchini
C L
CL
Neves
R H
RH
Machado-Silva
J R
JR
D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use*; D018753:Diet, Protein-Restricted; D005260:Female; D022781:Hepatocytes / Q000473:pathology; D008099:Liver / Q000469:parasitology / Q000473:pathology*; D008103:Liver Cirrhosis / Q000473:pathology; D051379:Mice; D011223:Praziquantel / Q000627:therapeutic use*; D012555:Schistosomiasis mansoni / Q000188:drug therapy* / Q000473:pathology; D012709:Serum Albumin / Q000032:analysis; D015430:Weight Gain
D000871:Anthelmintics; D012709:Serum Albumin; D011223:Praziquantel