title: Electromagnetic and thermal evaluation of an applicator specialized to permit high-resolution non-perturbing optical evaluation of cells being irradiated in the W-band.
abstract: To permit epi-illuminated, high-resolution optical microscopy of cells in monolayer culture during unperturbed W-band (75-110 GHz) irradiation, a new class of applicator has been developed based upon WR10 rectangular waveguide components: the cells are normally plated onto the underside of a coverslip which is then placed against the under side of a waveguide flange and receives a roughly circular exposure pattern, with the +/-1 dB central spot roughly 1 mm in diameter. Constructed and tested with 94 GHz millimeter waves, water-immersion optics, and free-convection cooling, the applicator works robustly and permits SARs at the cell layer as high as 4500 W/kg before the steady-state temperature rise at the cell layer exceeds 0.5 K.
authors.affiliation: Department of Electrical and Systems Engineering, Washington University, Saint Louis, MO 63130, USA. wfp@ese.wustl.edu
authors.forename: William F
authors.initials: WF
authors.lastname: Pickard
authors.forename: Eduardo G
authors.initials: EG
authors.lastname: Moros
authors.forename: Gal
authors.initials: G
authors.lastname: Shafirstein
mesh_terms: D000465:Algorithms; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003198:Computer Simulation; D004574:Electromagnetic Fields; D004581:Electronics / Q000295:instrumentation; D004867:Equipment Design; D020342:Finite Element Analysis; D007259:Infrared Rays; D008853:Microscopy / Q000295:instrumentation*; D008872:Microwaves; D008954:Models, Biological; D055096:Optical Devices; D055095:Optics and Photonics / Q000295:instrumentation*; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D012965:Sodium Chloride