Prevalence and level of Escherichia coli O157:H7 in feces and on hides of feedlot steers fed diets with or without wet distillers grains with solubles.
The objective of this study was to determine if wet distillers grains with solubles (WDGS) from corn in diets affected Escherichia coli O157:H7 in growing and finishing cattle; steers (n = 603) were randomly assigned to diets with or without WDGS. Hide and fecal samples were collected monthly (October through June) from each animal for enumeration and enrichment of E. coli O157:H7. In the growing phase (0 or 13.9% WDGS diets), fecal prevalence for E. coli O157:H7 in steers fed a diet with WDGS was twice that of the prevalence in control steers (P < 0.001). In the finishing phase (0 or 40% WDGS diets), the average prevalence in feces (P < 0.001) and on hides (P < 0.001) was higher for cattle fed WDGS. The average percentage of fecal E. coli O157:H7 enumerable samples during the finishing phase for cattle fed WDGS was 2.7% compared with 0.1% for control steers (P < 0.001). The average percentage of E. coli O157:H7 enumerable hide samples was not different between diets, but the cattle fed WDGS had higher levels (P < 0.05) of the pathogen. Animals fed WDGS had higher levels of E. coli (P < 0.001), higher pH values (P < 0.001), and lower concentrations of L-lactate (P < 0.001) in feces than those values of the control steers. These results indicate that feeding 40% WDGS could increase the level and prevalence of E. coli O157:H7 in and on feedlot cattle when E. coli O157:H7 is seasonally low.
Wells
J E
JE
U.S. Department of Agriculture, Agricultural Research Station, U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA. jim.wells@ars.usda.gov
Shackelford
S D
SD
Berry
E D
ED
Kalchayanand
N
N
Guerini
M N
MN
Varel
V H
VH
Arthur
T M
TM
Bosilevac
J M
JM
Freetly
H C
HC
Wheeler
T L
TL
Ferrell
C L
CL
Koohmaraie
M
M
D000821:Animal Feed / Q000382:microbiology*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D015169:Colony Count, Microbial; D002523:Edible Grain; D019453:Escherichia coli O157 / Q000254:growth & development* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006197:Hair / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008297:Male; D015995:Prevalence; D011897:Random Allocation; D012621:Seasons; D012995:Solubility; D003313:Zea mays