title: [Effects of high-density lipoprotein 1 on the formation of foam cells from human monocyte-derived macrophages].
abstract: OBJECTIVE
  To investigate effects of serum HDL(1) on the formation of foam cells from human peripheral blood monocyte-derived macrophages.
  
  
  METHODS
  Sectie density polyacrylamide gel electrophoresis (sd-PAGE) was applied for isolation and preparation of HDL(1) simultaneously. Monocytes were isolated from human peripheral blood by Ficoll-Hypaque density gradient centrifugation and plastic adsorptive process. The isolated monocytes were stimulated by phorbol 12-myristate 13-acetate (PMA) at a concentration of 50 nmol/L for 48 h and transferred to macrophages. The monocyte-derived macrophages were then coincubated with 80 mg/L ox-LDL and HDL(1) (0, 0.1, 1.0 and 10.0 mg/L) for 6, 12 and 24 h, respectively. The formation of foam cells was identified by transmission electron microscope (TEM), total cholesterol (TC), free cholesterol (FC) and protein (Pro) in cultured cells were quantitatively analyzed by high performance chromatography (HPLC) and modified lowry protein assay, respectively.
  
  
  RESULTS
  HDL(1) isolated from human serum by sd-PAGE could significantly decrease TC/Pro ratio in foam cells in a concentration-dependent (0 mg/L: 36.9 +/- 1.1, 10.0 mg/L: 6.2 +/- 0.4, P < 0.01) and time-dependent (10.0 mg/L HDL(1) 6 h: 16.9 +/- 0.9, 24 h: 6.4 +/- 0.6, P < 0.01) manner.
  
  
  CONCLUSIONS
  HDL(1) is capable of inhibiting and attenuating the formation of foam cells by decreasing cellular TC, therefore, might play an important role in attenuating atherosclerosis.
authors.affiliation: Department of Cardiology, First People's Hospital of Yulin, Guangxi Medical University, Yulin 537000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Liang
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Liu
authors.forename: Yong-Yuan
authors.initials: YY
authors.lastname: Liu
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Fei
authors.initials: F
authors.lastname: Miao
mesh_terms: D050197:Atherosclerosis; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000378:metabolism; D005487:Foam Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008077:Lipoproteins, LDL; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism
chemical_list: D008078:Cholesterol, LDL; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; C035392:high density lipoprotein-1; C099252:oxidized low density lipoprotein