Reduced formation of advanced glycation endproducts via interactions between glutathione peroxidase 3 and dihydroxyacetone kinase 1.
Dihydroxyacetone (DHA) induces the formation of advanced glycation endproducts (AGEs), which are involved in several diseases. Earlier, we identified dihydroxyacetone kinase 1 (Dak1) as a candidate glutathione peroxidase 3 (Gpx3)-interacting protein in Saccharomyces cerevisiae. This finding is noteworthy, as no clear evidence on the involvement of oxidative stress systems in DHA-induced AGE formation has been found to date. Here, we demonstrate that Gpx3 interacts with Dak1, alleviates DHA-mediated stress by upregulating Dak activity, and consequently suppresses AGE formation. Based on these results, we propose that defense systems against oxidative stress and DHA-induced AGE formation are related via interactions between Gpx3 and Dak1.
Lee
Hana
H
Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
Chi
Seung Wook
SW
Lee
Phil Young
PY
Kang
Sunghyun
S
Cho
Sayeon
S
Lee
Chong-Kil
CK
Bae
Kwang-Hee
KH
Park
Byoung Chul
BC
Park
Sung Goo
SG
D004098:Dihydroxyacetone / Q000378:metabolism*; D017353:Gene Deletion; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000096:biosynthesis* / Q000235:genetics; D018384:Oxidative Stress; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D017127:Glycation End Products, Advanced; D029701:Saccharomyces cerevisiae Proteins; D005979:Glutathione Peroxidase; C494438:HYR1 protein, S cerevisiae; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase; D004098:Dihydroxyacetone