title: [Mechanisms of protective action of bacterial intracellular metabolites for lowering of bactericidal effect of hydroxyl radicals].
abstract: OBJECTIVE
  To study mechanisms of attenuation of bactericidal effect of hydroxyl radicals by bacterial intracellular metabolites.
  
  
  METHODS
  Culture liquids of 16 strains of Lactobacillus spp., 21 strains of Corynebacterium spp., 8 strains of Micrococcus spp. and 17 strains of Staphylococcus spp. and fractions obtained from them were used. Bactericidal effect of hydroxyl radicals on Escherichia coli was measured by survival of the bacteria.
  
  
  RESULTS
  High prevalence of ability of bacteria to prevent other microorganisms from bactericidal action of hydroxyl radicals produced in Fenton's reaction was revealed. This effect was accompanied by other two: ability to oxidize Fe2+ in Fe3+ and production of substances with antioxidant properties - extracellular polysaccharides, pigments and lipid-containing compounds.
  
  
  CONCLUSIONS
  It was assumed that defense of microorganisms composing microbiocenosis from toxic effect of hydroxyl radicals realizes through production of bacterial metabolites which inactivate hydroxyl radicals or prevent their formation.
authors.forename: A V
authors.initials: AV
authors.lastname: Sgibnev
authors.forename: S V
authors.initials: SV
authors.lastname: Cherkasov
authors.forename: O V
authors.initials: OV
authors.lastname: Bukharin
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007501:Iron / Q000037:antagonists & inhibitors; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D010860:Pigments, Biological / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003470:Culture Media; C045076:Fenton's reagent; D005290:Ferric Compounds; D005296:Ferrous Compounds; D010860:Pigments, Biological; D011135:Polysaccharides, Bacterial; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D007501:Iron