title: Genome of a virulent bacteriophage Lb338-1 that lyses the probiotic Lactobacillus paracasei cheese strain.
abstract: There is a lack of fundamental knowledge about the influence of bacteriophage on probiotic bacteria and other commensals in the gut. Here, we present the isolation and morphological and genetic characterization of a virulent narrow-host-range bacteriophage, phiLb338-1. This phage was isolated from fresh sewage and was shown to infect the probiotic cheese strain Lactobacillus paracasei NFBC 338. Electron microscopy studies revealed that phiLb338-1 is a member of the Myoviridae family, with an isometric head, a medium-sized contractile tail, and a complex base plate. Genome sequencing revealed a 142-kb genome with 199 open reading frames. Putative functions could be assigned to 22% of the open reading frames; these had significant homology to genes found in the broad-host-range SPO1-like group of phages which includes the Enterococcus faecalis phage phiEF24C, Listeria phage A511, and Lactobacillus plantarum phage LP65. Interestingly, no significant genomic similarity was observed between the phage and the probiotic host strain. Future studies will determine if the presence of bacteriophage phiLb338-1 or others in the human or animal gut plays an antagonistic role against the probiotic effect of beneficial bacteria.
authors.affiliation: Biotechnology Department, Moorepark Food Research Centre, Teagasc-Moorepark, Cork, Ireland.
authors.forename: Debebe
authors.initials: D
authors.lastname: Alemayehu
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Orla
authors.initials: O
authors.lastname: O'Sullivan
authors.forename: Aidan
authors.initials: A
authors.lastname: Coffey
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: Olivia
authors.initials: O
authors.lastname: McAuliffe
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity / Q000648:ultrastructure; D016679:Genome, Viral; D023281:Genomics; D007778:Lactobacillus / Q000821:virology*; D008854:Microscopy, Electron; D010802:Phylogeny; D019936:Probiotics; D015678:Viral Structural Proteins / Q000378:metabolism; D014774:Virulence
chemical_list: D015678:Viral Structural Proteins