Antigenic properties of the coat of Cucumber mosaic virus using monoclonal antibodies.
The coat protein (CP) of Cucumber mosaic virus (CMV) was characterized by antigen-capture-ELISA using a panel of monoclonal antibodies (mAbs) which were produced against Pepo-CMV-CP. Comparative analysis of three mAbs with four different strains by competitive ELISA revealed that the binding affinity of the mAb decreased about 10-fold with both MY17- and Y-CMV than with Pepo-CMV. The CP of these three strains showed high homology (approximately 98%) following comparison in the GenBank database. CMV has a negatively charged loop structure, the betaH-betaI loop, although the amino acid at position 193 is not conserved. In addition, an amino acid residue identified within the variable region spanning amino acids 191-198, specifically at position 194, showed significant changes in Threonine, Alanine, Alanine, and Lysine of the Pepo-, MY17-, Y-, and M2-CMV strains, respectively. Evidence from competitive ELISA and GenBank database amino acid residues, when taken together, provide strong support suggesting that the dominant epitope site of CMV-CP-specific mAbs is the betaH-betaI loop 191-198. The four mAbs were chosen because they represent distinct, overlapping epitopes within the group-specific determinant located on the CMV-CP and because they all recognize linear epitopes. Knowledge of specific immunoglobulin genes for a common epitope may lead to insight on pathogen-host co-evolution and may help prevent virus infection in plants.
Zein
Haggag S
HS
Department of Genetics, Faculty of Agriculture, Cairo University, 12613, Egypt 12 Gamma Street, Giza 12613, Egypt. drzein@cu.edu.eg
Teixeira da Silva
Jaime A
JA
Miyatake
Kazutaka
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000914:Antibodies, Viral / Q000276:immunology / Q000378:metabolism; D000956:Antigens, Viral / Q000276:immunology* / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D017799:Cucumovirus / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D014026:Nicotiana / Q000821:virology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes