title: Both substrate availability and utilisation contribute to the defence of core temperature in response to acute cold.
abstract: Acute cooling significantly increases energy demand in non-hibernators for the defence of core temperature but the contribution of the liver to thermogenesis is poorly understood. A two-tracer method to estimate lipid metabolism in cold-naïve control (CON) and cold-acclimated (CA) rats was employed to quantify hepatic rates of fat metabolism. Both fenofibrate, to increase liver mass and fat oxidation and dichloroacetate (DCA) to inhibit fat oxidation were used to alter lipid metabolism in CON animals. Following acute cooling, CA led to a doubling of the time to reach a core temperature 25 degrees C (P<0.001), whereas DCA treatment decreased time of cooling (P<0.01). DCA-treatment increased the gradient of Arrhenius-transformed rate-pressure product (P<0.01). CA increased both palmitate uptake (P<0.001) and beta-oxidation (P<0.01) whilst DCA treatment decreased uptake (P<0.01) and beta-oxidation (P<0.05). Tissue-specific estimates of metabolism revealed that CA led to a 12-fold increase in beta-oxidation for brown adipose tissue (P<0.001) whilst fenofibrate halved beta-oxidation in the liver (P<0.01) despite doubling the liver mass (P<0.001) and DCA decreased hepatic beta-oxidation to 15% of control levels. Taken together, these results suggest that the liver has minimal contribution to thermogenesis in the rat, with brown adipose tissue significantly increasing both fat uptake and oxidation in response to CA.
authors.affiliation: School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom. d.hauton@bham.ac.uk
authors.forename: David
authors.initials: D
authors.lastname: Hauton
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Coney
authors.forename: Stuart
authors.initials: S
authors.lastname: Egginton
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001831:Body Temperature; D003080:Cold Temperature; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats
chemical_list: D005230:Fatty Acids, Nonesterified