Effects of zearalenone and alpha-Zearalenol in comparison with Raloxifene on T47D cells.
Zearalenone (Zen) is a mycotoxin with estrogenic effect which contaminates cereals. In cell culture, Zen and its metabolite, alpha-Zearalenol (alpha-Zel), stimulate breast cancer cells growth. Today hormone-dependent cancers are important because of high incidence and death rate. Previous studies showed that Zen and alpha-Zel have an effect on hormone-dependent cancers. This study explains the effects of the mentioned compounds in comparison with Raloxifene as an anti-estrogen. Cell culture technique was used with MDA-MB-231 and T47D cells for evaluation of compounds. MDA-MB-231 cells were used as negative control and also for proving that treatment compounds merely affect, due to their proliferation activity in the applied doses. According to the Resazurine-based method, for toxicity assay, none of the test compounds have an effect on MDA-MB-231 cells but do effect the growth of T47D cells. Zen and alpha-Zel at low concentrations (10-8-10-9 M) stimulated T47D cell growth and Raloxifene strongly inhibited cell growth induced by Zen and alpha-Zel. There is a noticeable result in controlling diet of hormonal carcinogenic compounds and applying novel anti-estrogens for prevention and treatment of hormone-dependent cancers.
Khosrokhavar
Roya
R
Food and Drug Laboratory Research Center (FDLRC), Ministry of Health (MOH), Tehran, Iran.
Rahimifard
Nahid
N
Shoeibi
Shahram
S
Hamedani
Morteza Pirali
MP
Hosseini
Mir-Jamal
MJ
D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D009183:Mycotoxins / Q000494:pharmacology*; D020849:Raloxifene Hydrochloride / Q000494:pharmacology*; D015025:Zearalenone / Q000494:pharmacology*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000494:pharmacology
D050071:Bone Density Conservation Agents; D009183:Mycotoxins; C029659:zearalenol; D020849:Raloxifene Hydrochloride; D015025:Zearalenone; D015029:Zeranol