[Expression of B7-related protein-1 on human coronary artery endothelial cells and its clinical significance].
OBJECTIVE
To study the effect of oxidized low density lipoprotein (ox-LDL) on the expression of B7-related protein-1 (B7RP-1) on human coronary artery endothelial cells (HCAECs).


METHODS
HCAECs were incubated in the presence of 100 mg/L ox-LDL for 24 h, and B7RP-1 expression levels were determined using fluorescence reverse transcription PCR (RT-PCR) and Western blotting.


RESULTS
B7RP-1 expression was detected HCAECs, with spots of fluorescence signals distributing on the cell membrane as observed under fluorescence microscope. RT-PCR with B7RP-1 specific primers yielded products of expected size (496 bp). Western blotting identified B7RP-1 expression in the HCAECs as a cell-associated protein with an apparent molecular mass of 70,000. Treatment of the cells with ox-LDL significantly increased B7RP-1 expression at both the mRNA and protein levels (P<0.05).


CONCLUSIONS
B7RP-1 is expressed on the membrane of HCAECs. ox-LDL can promote up-regulate the expression of B7RP-1, which might be one of the immunopathogenesis of atherosclerosis.
Xu
Lin
L
Department of Cardiology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.
Li
Zhi-liang
ZL
Hong
Chang-jiang
CJ
He
Jian-xin
JX
Ma
Jun
J
Xiang
Ding-cheng
DC
Zhang
Jie
J
Pan
Chun-mei
CM
Qiu
Jian
J
D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003331:Coronary Vessels / Q000166:cytology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand; D008077:Lipoproteins, LDL / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018122:B7-1 Antigen; D060945:Inducible T-Cell Co-Stimulator Ligand; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein