Polymorphism distribution and structural conservation in RNA-sensing Toll-like receptors 3, 7, and 8 in pigs.
BACKGROUND
Viral genomic RNA-both single-stranded (ss) and double-stranded (ds)-is recognized by RNA-sensing Toll-like receptors (TLRs), notably TLR3 (dsRNA), TLR7 (ssRNA), and TLR8 (ssRNA). However, our knowledge of the roles of porcine TLR3, 7, and 8 in antiviral immunity is inadequate.


METHODS
From information on exon-intron boundaries obtained through comparisons of the genomic and cDNA sequences, polymorphisms in the coding sequences of each gene were detected in 84 male pigs of 11 breeds.


RESULTS
Genomic structures are conserved between pigs and humans. The RNA-sensing TLR genes had fewer polymorphisms causing amino acid alterations than did the cell-surface TLR genes, but the alterations were distributed with a similar bias toward ectodomains.


CONCLUSIONS
The low level of diversity of substitutive polymorphisms in RNA-sensing TLRs than cell-surface ones implies that polymorphisms severely affecting function have been eliminated by selection pressure during longstanding pig breeding.


CONCLUSIONS
Recognition of virus-derived RNA is critical in host defense against infection. These results should provide a useful clue to analysis of the association between polymorphisms in RNA-sensing TLRs and disease resistance.
Morozumi
Takeya
T
Animal Genome Research Progmram (NIAS/STAFF), Tsukuba, Ibaraki, Japan.
Uenishi
Hirohide
H
D000818:Animals; D017124:Conserved Sequence; D006801:Humans; D008297:Male; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012367:RNA, Viral / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics*; D051196:Toll-Like Receptor 3 / Q000235:genetics*; D051199:Toll-Like Receptor 7 / Q000235:genetics*; D051200:Toll-Like Receptor 8 / Q000235:genetics*
D012367:RNA, Viral; D051196:Toll-Like Receptor 3; D051199:Toll-Like Receptor 7; D051200:Toll-Like Receptor 8