title: Comparison of the sealing ability of three filling techniques in canals shaped with two different rotary systems: a bacterial leakage study.
abstract: OBJECTIVE
  This study compared the sealing ability of 3 current filling techniques in root canals shaped with 2 different rotary systems.
  
  
  METHODS
  Eighty human extracted mandibular premolars were divided randomly into 2 similar groups of 40 each and instrumented with either ProTaper (Dentsply Maillefer, Tulsa, OK; group A) or Mtwo (VDW; Antaeos, Munich, Germany; group B) rotary systems. Each group was divided into 3 subgroups (n = 10) and 2 control groups (n = 5). Canals were filled either with the tapered single-cone technique (SC; subgroups A1 and B1), with lateral condensation (LC; subgroups A2 and B2), or warm vertical compaction (WVC; subgroups A3 and B3). AH Plus was used as a root canal sealer in all groups. Samples were sterilized in an ethylene oxide sterilizer for 12 hours. The apical 3-4 mm of the roots were immersed in brain-heart infusion culture medium with phenol red indicator within culture chambers. The coronal access of each specimen was inoculated every 48 hours with a suspension of Enterococcus faecalis. Bacterial leakage was monitored every 24 hours for 8 weeks. The data obtained were analyzed using a chi-squared test, and P was set at .05.
  
  
  RESULTS
  In group A, 70% of the specimens filled with SC (subgroup A1), 50% of the specimens filled with LC (subgroup A2), and 20% of the specimens filled with WVC (subgroup A3) leaked. There was no statistically significant difference between the subgroups (P > .05). In group B, bacterial leakage was observed in 50% of SC samples (subgroup B1), 40% of LC samples (subgroup B2), and 50% of WVC samples (subgroup B3). There was no statistically significant difference between subgroups B1, B2, and B3 (P > .05). There was also no statistically significant difference between group A and group B (P > .05).
  
  
  CONCLUSIONS
  Filling with SC, LC, and WVC techniques in canals treated with ProTaper or Mtwo rotary instruments showed similar levels of sealing efficacy.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Tamer
authors.initials: T
authors.lastname: Taşdemir
authors.forename: Kürşat
authors.initials: K
authors.lastname: Er
authors.forename: Tahsin
authors.initials: T
authors.lastname: Yildirim
authors.forename: Kurtulus
authors.initials: K
authors.lastname: Buruk
authors.forename: Davut
authors.initials: D
authors.lastname: Celik
authors.forename: Sabri
authors.initials: S
authors.lastname: Cora
authors.forename: Erhan
authors.initials: E
authors.lastname: Tahan
authors.forename: Behram
authors.initials: B
authors.lastname: Tuncel
authors.forename: Ahmet
authors.initials: A
authors.lastname: Serper
mesh_terms: D001840:Dental Bonding; D003763:Dental Leakage / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology / Q000473:pathology*; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D004853:Epoxy Resins / Q000627:therapeutic use; D004867:Equipment Design; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D008422:Materials Testing; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D012389:Root Canal Obturation / Q000379:methods*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D019227:Tooth Apex / Q000382:microbiology / Q000473:pathology
chemical_list: D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C534916:epoxy resin-based root canal sealer; D006171:Gutta-Percha