title: The activity of lysosomal exoglycosidases in serum of alcohol-dependent men supplemented with borage oil enriched with vitamin E.
abstract: The aim of this study was to determine the activity of the lysosomal exoglycosidases: alpha-mannosidase (MAN), alpha-fucosidase (FUC), and beta-glucuronidase (GLUCUR) in serum of alcohol-dependent men supplemented and not supplemented with borage oil enriched with vitamin E. Serum was collected from eight social drinkers and 16 alcohol-dependent men after a drinking period. The activity of exoglycosidases and the concentration of protein in serum were determined. The increase in specific activity of MAN and GLUCUR was significant in serum of alcohol-dependent men both not supplemented and supplemented with borage oil enriched with vitamin E, in comparison with the specific activity in serum of social drinkers. In serum of alcohol-dependent men treated with borage oil enriched with vitamin E, specific activity of MAN and GLUCUR fluctuated in comparison with alcohol-dependent men not supplemented. Specific activity of FUC in serum of alcohol-dependent men both not supplemented and supplemented with borage oil enriched with vitamin E showed a tendency to increase, in comparison with social drinkers. Specific activity of FUC had a tendency to decrease in serum of alcohol-dependent men supplemented with borage oil enriched with vitamin E, in comparison with alcohol-dependent men not supplemented. Thus, supplementation of alcohol-dependent men after a long-lasting drinking period with borage oil and vitamin E did not change the rate of catabolism of the oligosaccharide chains of glycoconjugates, as evaluated by serum activity of exoglycosidases.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Poland. ag.zaniewska@wp.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zaniewska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Borzym-Kluczyk
authors.forename: Slawomir D
authors.initials: SD
authors.lastname: Szajda
authors.forename: Jacek
authors.initials: J
authors.lastname: Romatowski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gil
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Knas
authors.forename: Jacek
authors.initials: J
authors.lastname: Dobryniewski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zwierz
mesh_terms: D000437:Alcoholism / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D029683:Borago / Q000737:chemistry*; D019587:Dietary Supplements; D006026:Glycoside Hydrolases / Q000097:blood*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D010938:Plant Oils / Q000494:pharmacology / Q000627:therapeutic use*; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use*; D017965:gamma-Linolenic Acid / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D010938:Plant Oils; D014810:Vitamin E; D017965:gamma-Linolenic Acid; D006026:Glycoside Hydrolases; C079363:borage oil