Ascaris lumbricoides: alteration of the erythrocyte superficial charge using the Partition Method in aqueous two-phase system.
Sialic acid is responsible for the negative charge of the erythrocyte. The decrease of sialic acid has hemodynamical and hemorheological importance. The aim was to study the effect of A. lumbricoides on the erythrocyte superficial charge using the Partition Method in aqueous two-phase system in order to indirectly evaluate the alteration of sialic acid in the red cells. We worked with five parasite extracts (AE) and larvae concentrate (LC). Erythrocyte superficial charge was studied by working with non-treated (Controls) and treated erythrocytes. The treatment consisted of incubating the erythrocytes with AE or LC for 30 minutes at 4 degrees C, 20 degrees C and 37 degrees C. The red cells were separated in a sensitive charge two-phase system (Dx/ PEG). The partition coefficient (P) of treated and untreated erythrocytes were calculated. The results showed a P decrease at the three temperatures for red cells treated with four of the AE. The remaining extract did change P values at any of the temperatures studied. The erythrocytes treated with LC showed a decrease in the P value at 37 degrees C and 4 degrees C but no change was observed at 25 degrees C. Statistical analysis concluded that P values were significantly lower in treated erythrocytes than in their corresponding untreated ones (p < 0.05). The Partition Method showed that this parasite alters the erythrocyte superficial charge which may indicate that it can catch sialic acid.
Ponce de León
Patricia
P
Laboratorio de Parasitología, Laboratorio de Inmunohematología, Inmunorreología e Inmunogenética, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina. tefu1958@hotmail.com
Lebensohn
Natalia
N
Foresto
Patricia
P
Valverde
Juana
J
D000818:Animals; D017164:Ascaris lumbricoides / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000502:physiology; D004912:Erythrocytes / Q000737:chemistry* / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology*; D008954:Models, Biological; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D009994:Osmolar Concentration
D019158:N-Acetylneuraminic Acid