Physiological levels of glutathione enhance Zn(II) binding by a Cys4 zinc finger.
Using fluorescence and UV-vis spectroscopies and mass spectrometry, we demonstrated that the presence of physiological levels of reduced glutathione enhances the binding of Zn(II) to XPAzf, a Cys4 zinc finger peptide derived from the XPA protein, by means of formation of a ternary complex of a general formula ZnXPAzf[GSH]. Similar complexes were also indicated by ESI-MS for isostructural Co(II)- and Cd(II)-substituted XPAzf. The observed enhancement of the Zn(II) binding to XPAzf by a factor of 50 over the physiological range of GSH concentrations of 1-20 mM corresponds to a dissociation constant of GSH from the ZnXPAzf[GSH] complex of 0.05 microM. This effect may account for an apparent discrepancy between relatively low Zn(II) binding constants measured in vitro for many zinc fingers, and the requirement of tight Zn(II) binding enforced by intracellular zinc buffering by the thionein/metallothionein couple.
Piatek
Katarzyna
K
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland. katarzina@gmail.com
Hartwig
Andrea
A
Bal
Wojciech
W
D000818:Animals; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D051760:Xeroderma Pigmentosum Group A Protein / Q000737:chemistry / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry / Q000378:metabolism*; D016335:Zinc Fingers
D010455:Peptides; D051760:Xeroderma Pigmentosum Group A Protein; D005978:Glutathione; D015032:Zinc; D003545:Cysteine