[Effects of epigallocatechin-3-gallate on the proliferation of colonic cancer cell line SW620 and PAK1 gene expression].
OBJECTIVE
To investigate the effects of epigallocatechin-3-gallate (EGCG) on the proliferation of SW620 cells and the expression of PAK1 gene.


METHODS
Human colonic cancer cell line SW620 was treated with EGCG at 40, 60 and 80 micromol/L and cultured in RPMI 1640 medium for 0, 24, 48 and 72 h. The proliferation of SW620 cells was observed by MTT assay before and after EGCG treatment, and the expression of PAK1 protein was observed by Western blotting.


RESULTS
SW620 cells treated with EGCG displayed a slowed growth in comparison with the control cells, and the growth rate decreased with the increase of EGCG concentration. PAK1 protein expression was lowered in SW620 cells after EGCG treatment for 48 h.


CONCLUSIONS
EGCG can inhibit the proliferation and partially reduce the expression of PAK1 protein in SW620 cells.
Chen
Xue-qing
XQ
Department of Gastroenterology, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
Wu
Jin-bao
JB
Yang
Xiao-qiang
XQ
Song
Yu-gang
YG
Li
Ming-song
MS
D015153:Blotting, Western; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D054462:p21-Activated Kinases / Q000378:metabolism*
D002392:Catechin; C045651:epigallocatechin gallate; D054462:p21-Activated Kinases