Screening for reactive metabolites using electro-optical arrays featuring human liver cytosol and microsomal enzyme sources and DNA.
We demonstrate for the first time the combination of human liver cytosol and microsomal enzyme sources into an electro-optical array to screen for reactive metabolites produced in multi-enzyme metabolic processes.
Zhao
Linlin
L
Department of Chemistry, University of Connecticut, 55 N. Eagleville Rd, Storrs, CT, USA.
Schenkman
John B
JB
Rusling
James F
JF
D000123:Acetyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D001711:Biotransformation; D003600:Cytosol / Q000201:enzymology*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018736:DNA Adducts / Q000032:analysis / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods; D015946:Ethylene Dibromide / Q000378:metabolism; D005449:Fluorenes / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D008163:Luminescent Measurements / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D008862:Microsomes, Liver / Q000201:enzymology*; D053758:Nanoparticles / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D018736:DNA Adducts; D005449:Fluorenes; D009942:Organometallic Compounds; D004364:Pharmaceutical Preparations; C492100:bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))ruthenium(II); D015946:Ethylene Dibromide; C012177:2-aminofluorene; D004247:DNA; D000123:Acetyltransferases; D005982:Glutathione Transferase; D005978:Glutathione