title: Temporal changes of angiopoietins and Tie2 expression in rat lungs after monocrotaline-induced pulmonary hypertension.
abstract: Angiogenic factors such as vascular endothelial growth factor (VEGF) are implicated in pulmonary hypertension (PH). However, the pathway of angiogenic factor-mediated pathologic angiogenesis in PH remains unclear. In this study, we evaluated the temporal expression of angiopoietin (Ang) 1, Ang2, and their receptor (Tie2) as well as VEGF, endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), and heme oxygenase 1 (HO1) in the monocrotaline-induced PH model. Histologic evaluation showed pathologic vascular remodeling in the arteries of lung sections 1 wk after monocrotaline treatment. Protein levels of Ang1, Ang2, eNOS, iNOS, HO1, and VEGF were increased 1 wk after monocrotaline treatment but Tie2 protein levels were decreased 2 wk afterward. These results suggest that Ang2 mediates vascular remodeling in PH by decreasing Tie2 expression. Therefore, the Ang-Tie2 system may play a role in the pathophysiology of PH.
authors.affiliation: Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Gyeongnam, Republic of Korea.
authors.forename: Yu Ji
authors.initials: YJ
authors.lastname: Cho
authors.forename: Jae Yoon
authors.initials: JY
authors.lastname: Han
authors.forename: Sang Gab
authors.initials: SG
authors.lastname: Lee
authors.forename: Byeong Tak
authors.initials: BT
authors.lastname: Jeon
authors.forename: Wan Sung
authors.initials: WS
authors.lastname: Choi
authors.forename: Young Sil
authors.initials: YS
authors.lastname: Hwang
authors.forename: Gu Seob
authors.initials: GS
authors.lastname: Roh
authors.forename: Jong Deog
authors.initials: JD
authors.lastname: Lee
mesh_terms: D042683:Angiopoietin-1 / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D006976:Hypertension, Pulmonary / Q000139:chemically induced / Q000378:metabolism*; D007150:Immunohistochemistry; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D016686:Monocrotaline / Q000633:toxicity*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D042787:Receptor, TIE-2 / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D042461:Vascular Endothelial Growth Factor A; D016686:Monocrotaline; D019001:Nitric Oxide Synthase; D042787:Receptor, TIE-2