Development of monoclonal antibodies for analyzing immune and hematopoietic systems of common marmoset.
OBJECTIVE
Common marmosets are considered experimental animals of primates useful for medical research. We developed several monoclonal antibodies (mAbs) directed to CD molecules to gain initial insight into the immune and hematopoietic systems of this organism, and analyzed the basic cellularity and characters of marmoset lymphocytes.


METHODS
Anti-marmoset CD antigen mAbs were prepared using marmoset antigen-expressing transfectants and used for flow cytometric analyses and cell fractionation. Expression of T-cell-related cytokine gene transcripts was examined in response to T-cell receptor stimulation by reverse transcription polymerase chain reaction analyses. Hematopoietic progenitor activities of marmoset bone marrow cells were examined in fractionated cells by mAbs against CD117 (c-kit) and CD34.


RESULTS
CD4 and CD8 expression profiles in T-cell subsets of marmoset were essentially similar to those in mouse and human. CD4(+) and CD8(+) subsets were isolated from marmoset spleens. Detected transcripts after stimulation of T cells included Th1-, Th2-, and Th17-related cytokines in CD4(+) cells and cytotoxic proteases in CD8(+) cells, respectively. Colony-forming abilities were detected mainly in CD117 (c-kit)(+) cells, irrespective of CD34 expression.


CONCLUSIONS
Marmoset immune system was basically similar to human and mouse systems.
Kametani
Yoshie
Y
Department of Immunology, Tokai University School of Medicine, Isehara, Japan.
Suzuki
Daisuke
D
Kohu
Kazuyoshi
K
Satake
Masanobu
M
Suemizu
Hiroshi
H
Sasaki
Erika
E
Ito
Toshio
T
Tamaoki
Norikazu
N
Mizushima
Tomoko
T
Ozawa
Manabu
M
Tani
Kenzaburo
K
Kito
Mitsuaki
M
Arai
Hideo
H
Koyanagi
Akemi
A
Yagita
Hideo
H
Habu
Sonoko
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D016466:CHO Cells; D002144:Callithrix / Q000097:blood / Q000276:immunology* / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D053804:Granzymes / Q000235:genetics; D006410:Hematopoiesis / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007107:Immune System / Q000276:immunology*; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D053804:Granzymes