title: Effects of overnight sleep restriction on brain chemistry and mood in women with unipolar depression and healthy controls.
abstract: BACKGROUND
  Partial or total overnight sleep deprivation produces immediate mood improvement in about 50% of patients with depression, but not in healthy controls. Our objectives were to compare the neurochemical changes that accompanied partial overnight sleep deprivation in healthy and depressed participants, and to compare baseline neurochemical profiles and overnight neurochemical changes between those depressed participants who did and did not respond to sleep loss with mood improvement.
  
  
  METHODS
  We studied 2 brain regions (left dorsal prefrontal area and pons) in 12 women with unipolar depression and in 15 healthy women using proton magnetic resonance spectroscopy acquired at 1.5 T. The scans took place at baseline and 24 hours later after a night with sleep restricted to a maximum of 2.5 hours (22:30-01:00). We assessed 3 neurochemical signals (referenced to internal water): N-acetylaspartate (NAA), choline compounds (Cho) and creatine-plus-phosphocreatine (tCr).
  
  
  RESULTS
  In both groups combined, sleep restriction caused a 20.1% decrease in pontine tCr (F(1-16) = 5.07, p = 0.039, Cohen's d = 0.54) and an 11.3% increase in prefrontal Cho (F(1-21) = 5.24, p = 0.033, Cohen's d = 0.46). Follow-up tests revealed that prefrontal Cho increases were significant only among depressed participants (17.9% increase, t(9) = -3.35, p = 0.008, Cohen's d = 1.06). Five depressed patients showed at least 30% improvement in mood, whereas 6 showed no change or worsening in mood after sleep restriction. Baseline pontine Cho levels distinguished subsequent responders from nonresponders to sleep restriction among depressed participants (z = 2.61, p = 0.008).
  
  
  CONCLUSIONS
  A limitation of this study is the relatively small sample size.
  
  
  CONCLUSIONS
  Sleep restriction altered levels of pontine tCr and prefrontal Cho in both groups combined, suggesting effects on phospholipid and creatine metabolism. Baseline levels of pontine Cho were linked to subsequent mood responses to sleep loss, suggesting a role for pontine phospholipid metabolism in mood effects of sleep restriction.
authors.affiliation: Department of Psychiatry, Dalhousie University, Halifax, NS.
authors.forename: Denise
authors.initials: D
authors.lastname: Bernier
authors.forename: Robert
authors.initials: R
authors.lastname: Bartha
authors.forename: Sivakumaran
authors.initials: S
authors.lastname: Devarajan
authors.forename: Frank P
authors.initials: FP
authors.lastname: Macmaster
authors.forename: Matthias H
authors.initials: MH
authors.lastname: Schmidt
authors.forename: Benjamin
authors.initials: B
authors.lastname: Rusak
mesh_terms: D000328:Adult; D000339:Affect; D000704:Analysis of Variance; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D003866:Depressive Disorder / Q000378:metabolism* / Q000523:psychology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010725:Phosphocreatine / Q000378:metabolism; D011149:Pons / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism*; D011522:Protons; D011581:Psychological Tests; D012892:Sleep Deprivation / Q000378:metabolism* / Q000523:psychology; D055815:Young Adult
chemical_list: D011522:Protons; D010725:Phosphocreatine; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline