title: Reversible inactivation of an intracellular uricase from Bacillus fastidiosus via dissociation of homotetramer into homodimers in solutions of low ionic strength.
abstract: An intracellular uricase from Bacillus fastidiosus with high catalytic capacity and strong resistance to xanthine was inactivated in water but could be essentially re-activated in solutions of high ionic strength. By polyacrylamide gel electrophoresis (PAGE), gradient PAGE, sodium-dodecyl-sulfate-PAGE, gel-filtration through Sephadex G200, and activity staining with peroxidase and its chromatogenic substrate, this homotetrameric uricase in water was found to dissociate into inactive homodimers that could form active homotetramers again in solutions of high ionic strength. Sensitivity to low ionic strength of solutions complicates formulation of this uricase as a drug and its elimination requires protein engineering.
authors.affiliation: Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Youquan
authors.initials: Y
authors.lastname: Bu
authors.forename: Ping
authors.initials: P
authors.lastname: Deng
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Feng
authors.forename: Yanling
authors.initials: Y
authors.lastname: Xie
authors.forename: Sha
authors.initials: S
authors.lastname: Zhu
authors.forename: Huidong
authors.initials: H
authors.lastname: Yuan
authors.forename: Ming'an
authors.initials: M
authors.lastname: Yu
authors.forename: Fei
authors.initials: F
authors.lastname: Liao
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D009994:Osmolar Concentration; D012996:Solutions; D014503:Urate Oxidase / Q000037:antagonists & inhibitors* / Q000737:chemistry
chemical_list: D012996:Solutions; D014503:Urate Oxidase