Delta-di-carboxybutyl phosphoramidate of 2'-deoxycytidine-5'-monophosphate as substrate for DNA polymerization by HIV-1 reverse transcriptase.
The replacement of the pyrophosphate moiety of 2'-deoxynucleoside triphosphates by non natural delta-dicarboxylic butyl amino acid allows incorporation of natural 2'-deoxycytidine into DNA using HIV-1 reverse transcriptase (RT) as enzyme. In contrast, the 3'-deoxycytidine analogue was not a substrate of the HIV-1 RT.
Zlatev
Ivan
I
Institut des Biomolécules Max Mousseron, UMR 5247, Université Montpellier 2, CC1704, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
Giraut
Anne
A
Morvan
François
F
Herdewijn
Piet
P
Vasseur
Jean-Jacques
JJ
D000577:Amides / Q000737:chemistry*; D000600:Amino Acids, Dicarboxylic / Q000737:chemistry*; D004261:DNA Replication; D003843:Deoxycytidine Monophosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D010756:Phosphoric Acids / Q000737:chemistry*; D013379:Substrate Specificity
D000577:Amides; D000600:Amino Acids, Dicarboxylic; D010756:Phosphoric Acids; D003843:Deoxycytidine Monophosphate; C011067:phosphoramidic acid; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase