title: Protein storage vacuoles of Brassica napus zygotic embryos  accumulate a BURP domain protein and perturbation  of its production distorts the PSV.
abstract: BNM2is a prototypical member of the enigmatic BURP domain protein family whose members contain the signature FX6-7GX10-28PX25-31CX11-12X2SX45-56CHX10 CHX25-29CHX2TX15-16PX5CH in the C-terminus. This protein family occurs only in plants, and the cognate genes vary very widely in their expression contexts in vegetative and reproductive tissues. None of theBURP family members has been assigned any biochemical function. BNM2 was originally discovered as a gene expressed in microspore derived embryos (MDE) of Brassica napus but we found that MDE do not contain the corresponding protein. We show that BNM2 protein production is confined to the seeds and localized to the protein storage vacuoles (PSV) even though the transcript is found in vegetative parts and floral buds as well. In developing seeds, transcript accumulation precedes protein appearance by more than 18 days. RNA accumulation peaks at approximately 20 days post anthesis (DPA) whereas protein accumulation reaches its maximum at approximately 40 DPA. Transgenic expression of BNM2 does not abrogate this regulation to yield ectopic protein production or to alter the temporal aspect ofBNM2 accumulation. Overexpression ofBNM2 led to spatial distortion of storage protein accumulation within PSV and to some morphological alterations of PSVs. However, the overall storage protein content was not altered.
authors.affiliation: Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, SK S7N 0W9, Canada.
authors.forename: Prapapan
authors.initials: P
authors.lastname: Teerawanichpan
authors.forename: Qun
authors.initials: Q
authors.lastname: Xia
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Caldwell
authors.forename: Raju
authors.initials: R
authors.lastname: Datla
authors.forename: Gopalan
authors.initials: G
authors.lastname: Selvaraj
mesh_terms: D029688:Brassica napus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055314:Seed Storage Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012639:Seeds / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D010940:Plant Proteins; D055314:Seed Storage Proteins