title: Alkaloids from the leaves of Daphniphyllum subverticillatum.
abstract: Four new alkaloids, 17-hydroxydaphnigraciline (1), subdaphnidine A (2), daphnezomine L methyl ester (3), and 11-hydroxycodaphniphylline (4), along with 24 known analogues, were isolated from the leaves of Daphniphyllum subverticillatum. The structures of 1-4 were elucidated on the basis of spectroscopic methods and from chemical evidence. Daphnilongeridine (5) showed cytotoxicity against several tumor cell lines at IC(50) values in the range 2.4-9.7 microM and against the HMEC human microvascular endothelial cell line with an IC(50) of 2.7 microM.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi-Tech Park, Shanghai, People's Republic of China.
authors.forename: Chuan-Rui
authors.initials: CR
authors.lastname: Zhang
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Liu
authors.forename: Teng
authors.initials: T
authors.lastname: Feng
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zhu
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Geng
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Yue
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007941:Leukemia P388; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D031995:Saxifragaceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal