Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins.
Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is difficult to detect Babesia organisms by observation of Giemsa-stained thin blood smears. We constructed a monoclonal phage display single chain antibody (scFv) against a B. gibsoni merozoite antigen, P50 protein. Intraerythrocytic B. gibsoni organisms are clearly stained using this antibody. The monoclonal scFv facilitated the detection of B. gibsoni organisms in canine blood samples.
Hirose
Yoshino
Y
Department of Veterinary Parasitological Diseases, Gifu University, Gifu, Japan.
Matsuhira
Saeka
S
Batanova
Tatiana Alexandrovna
TA
Goo
Youn-Kyoung
YK
Terkawi
Mohamad A
MA
Nishikawa
Yoshifumi
Y
Kitoh
Katsuya
K
Xuan
Xuenan
X
Takashima
Yasuhiro
Y
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001403:Babesia / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D001404:Babesiosis / Q000662:veterinary; D017931:DNA Primers; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D017125:Genes, Protozoan / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D013987:Ticks / Q000469:parasitology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; C434466:P50 protein, Babesia gibsoni; D019151:Peptide Library