title: Electrophoretic karyotyping and construction of a bacterial artificial chromosome library of the winter mushroom Flammulina velutipes.
abstract: An electrophoretic karyotype of Korean Flammulina velutipes was obtained using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The monokaryotic progenies (4019-20 and 4019-18) and a dikaryotic strain (4019-2018) had 6-8 chromosomes, with sizes ranging from 1.6 to 5.8 megabase (Mb) pairs. Among the 3 strains that were examined, strain 4019-20 resolved into at least 7 chromosomes, with a total genome size of approximately 26.7Mb. We selected several chromosome-specific genes from the cDNA library of F. velutipes using Southern hybridization analysis. In order to determine the whole genome sequence, we constructed a bacterial artificial chromosome (BAC) library of the 4019-20 strain. The BAC library comprised 3840 clones, and the insert size ranged from 60 to 228kb, with an average size of 136kb.
authors.affiliation: National Academy of Agricultural Science, RDA, Korea.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Park
authors.forename: Jong-Kun
authors.initials: JK
authors.lastname: Kim
authors.forename: Won-Sik
authors.initials: WS
authors.lastname: Kong
authors.forename: Eun-Sung
authors.initials: ES
authors.lastname: Song
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Hyungtae
authors.initials: H
authors.lastname: Kim
authors.forename: Jang-Ho
authors.initials: JH
authors.lastname: Hahn
authors.forename: Hee-Wan
authors.initials: HW
authors.lastname: Kang
authors.forename: Byoung-Moo
authors.initials: BM
authors.lastname: Lee
mesh_terms: D022202:Chromosomes, Artificial, Bacterial; D004586:Electrophoresis; D055393:Flammulina / Q000235:genetics*; D015723:Gene Library; D007621:Karyotyping