The design of biodegradable microcarriers for induced cell aggregation.
PLLA microspheres were aminolyzed in hexanediamine/propanol solution to introduce free amino groups on their surface, which were further transferred into aldehyde groups by a treatment of glutaraldehyde. Chitosan-graft-lactose was then covalently coupled via Schiff base formation. Morphological variation and chitosan-graft-lactose immobilization were characterized. In vitro culture of rabbit auricular chondrocytes demonstrated that the PLLA microcarriers could effectively support the cell attachment and particularly induce cell aggregation on their surface. The formed cell aggregates/microcarriers composite showed higher viability and extracellular matrix production. Thus, the PLLA microcarriers can be potentially used as an injectable delivery system for cartilage repair.
Tan
Huaping
H
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, and Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Wu
Jindan
J
Huang
Dejuan
D
Gao
Changyou
C
D000588:Amines / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002449:Cell Aggregation / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D006868:Hydrolysis / Q000187:drug effects; D019344:Lactic Acid / Q000494:pharmacology; D007785:Lactose / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008863:Microspheres; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology; D011817:Rabbits; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors
D000588:Amines; D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; D007785:Lactose