Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid.
Small non-coding RNAs of 18-25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3' end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1.
Yeung
Man Lung
ML
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0460, USA.
Bennasser
Yamina
Y
Watashi
Koichi
K
Le
Shu-Yun
SY
Houzet
Laurent
L
Jeang
Kuan-Teh
KT
D002460:Cell Line; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012357:RNA, Transfer, Lys / Q000378:metabolism; D022661:RNA, Untranslated / Q000737:chemistry* / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D013601:T-Lymphocytes / Q000821:virology; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012357:RNA, Transfer, Lys; D022661:RNA, Untranslated; D012367:RNA, Viral