title: Binding interaction of quinclorac with bovine serum albumin: a biophysical study.
abstract: Quinclorac (QUC) is a new class of highly selective auxin herbicides. The interaction between QUC and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy, synchronous fluorescence, three-dimensional fluorescence, CD spectroscopy and UV-vis absorption spectroscopy under simulative physiological condition. It was proved that the probable quenching mechanism of BSA by quinclorac was dynamic quenching. The Stern-Volmer quenching model has been successfully applied and the activation energy of the interaction as much as 8.03 kJ mol(-1), corresponding thermodynamic parameters DeltaH(theta), DeltaS(theta) and DeltaG(theta) were calculated. The results indicated that the acting forces between QUC and BSA were mainly hydrogen bonding and van der Waals forces. According to the Förster non-radiation energy transfer theory, the average binding distance between donor (BSA) and acceptor (QUC) was obtained (r=3.12 nm). The alterations of protein secondary structure in the presence of QUC were confirmed by the evidences from three-dimensional fluorescence, synchronous fluorescence and CD spectroscopy. Furthermore, the site marker competitive experiments indicated that the binding of QUC to BSA primarily took place in Sudlow site I.
authors.affiliation: State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xiao-Le
authors.initials: XL
authors.lastname: Han
authors.forename: Ping
authors.initials: P
authors.lastname: Mei
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.forename: Feng-Lei
authors.initials: FL
authors.lastname: Jiang
authors.forename: Ran
authors.initials: R
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D011485:Protein Binding; D011487:Protein Conformation; D011804:Quinolines / Q000032:analysis / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D011804:Quinolines; D012710:Serum Albumin, Bovine; C489048:quinclorac