title: In vivo assessment of macrophage CNS infiltration during disruption of the blood-brain barrier with focused ultrasound: a magnetic resonance imaging study.
abstract: Focused ultrasound has been discovered to locally and reversibly increase permeability of the blood-brain barrier (BBB). However, inappropriate sonication of the BBB may cause complications, such as hemorrhage and brain tissue damage. Tissue damage may be controlled by selecting optimal sonication parameters. In this study, we sought to investigate the feasibility of labeling cells with superparamagnetic iron oxide particles to assess the inflammatory response during focused-ultrasound-induced BBB opening. We show that infiltration of phagocytes does not occur using optimal parameters of sonication. Taken together, the results of our study support the usefulness and safety of focused-ultrasound-induced BBB opening for enhancing drug delivery to the brain. These findings may have implications for the optimization of sonication parameters.
authors.affiliation: Department of Electrical Engineering, Chang-Gung University, Taoyuan, Taiwan.
authors.forename: Hao-Li
authors.initials: HL
authors.lastname: Liu
authors.forename: Yau-Yau
authors.initials: YY
authors.lastname: Wai
authors.forename: Po-Hong
authors.initials: PH
authors.lastname: Hsu
authors.forename: Lee-Ang
authors.initials: LA
authors.lastname: Lyu
authors.forename: Jia-Shin
authors.initials: JS
authors.lastname: Wu
authors.forename: Chia-Rui
authors.initials: CR
authors.lastname: Shen
authors.forename: Jin-Chung
authors.initials: JC
authors.lastname: Chen
authors.forename: Tzu-Chen
authors.initials: TC
authors.lastname: Yen
authors.forename: Jiun-Jie
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D002138:Calibration; D002490:Central Nervous System / Q000166:cytology* / Q000000981:diagnostic imaging*; D005290:Ferric Compounds; D008264:Macrophages / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D008297:Male; D020556:Neutrophil Infiltration / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014463:Ultrasonography
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide