In vivo circular RNA production using a constitutive promoter for high-level expression.
The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.
Umekage
So
S
Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Aichi, Japan. umekage@eco.tut.ac.jp
Kikuchi
Yo
Y
D052157:Aptamers, Nucleotide / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation; D007438:Introns / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D000079962:RNA, Circular; D015183:Restriction Mapping
D052157:Aptamers, Nucleotide; D017931:DNA Primers; D012329:RNA, Bacterial; D000079962:RNA, Circular; D012313:RNA