Immobilization of avocado phytase on epoxy-activated Sepabead EC-EP and its application in soymilk phytate hydrolysis.
There is a great demand for using phytases to reduce phytate content in animal feed stuffs and food for human consumption. Industrial application of phytase requires efficient methods to immobilize the enzyme, yielding a biocatalyst with high activity and stability compared to the free enzyme. In the present study, phytase was immobilized on Sepabead EC-EP and then used in the biodegradation of soymilk phytate. The immobilized enzyme exhibited an activity of 0.1 U per g of carrier and activity yield of 70.83%. Optimum temperature and pH for the immobilized enzyme were 55 degrees C and 5.5, respectively. Both the enzymes were stable between pH 3.0-8.0 and below 70 degrees C. Kinetic parameters(K(m) and V(max)) and the usability of the immobilized enzyme were determined. The immobilized enzyme hydrolyzed 65% of soymilk phytate in 8 h at 60 degrees C, as compared with 56% hydrolysis observed for the native enzyme over the same period of time.
Celem
Evran Biçak
EB
Ege University, Faculty of Science, Biochemistry Department, Bornova-Izmir, Turkey.
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D010832:6-Phytase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D027421:Persea / Q000201:enzymology*; D010833:Phytic Acid / Q000378:metabolism*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012995:Solubility; D041942:Soy Milk / Q000737:chemistry*; D013696:Temperature
D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D010833:Phytic Acid; D010832:6-Phytase