title: Impact of phenolic substrate and growth temperature on the Arthrobacter chlorophenolicus proteome.
abstract: We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07 Uppsala, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Unell
authors.forename: Paul E
authors.initials: PE
authors.lastname: Abraham
authors.forename: Manesh
authors.initials: M
authors.lastname: Shah
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Christian
authors.initials: C
authors.lastname: Rückert
authors.forename: Nathan C
authors.initials: NC
authors.lastname: VerBerkmoes
authors.forename: Janet K
authors.initials: JK
authors.lastname: Jansson
mesh_terms: D001173:Arthrobacter / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002733:Chlorophenols / Q000494:pharmacology; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D009596:Nitrophenols / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002733:Chlorophenols; D009596:Nitrophenols; D010636:Phenols; D020543:Proteome; C029107:4-chlorophenol; C024836:4-nitrophenol