Evaluation of anti-colitic effect of lactic acid bacteria in mice by cDNA microarray analysis.
To evaluate the anti-colitic effect of lactic acid bacteria by cDNA microarray analysis, a lactic acid bacteria mixture (LM) consisting of Lactobacillus brevis HY7401, L. suntoryeus HY7801 and Bifidobacterium longum HY8004 was orally administered to dextran sulfate (DSS)-induced colitic mice and the expression profile of numerous genes was assessed. DSS treatment caused colitic outcomes such as inflammation and colon shortening. DSS also up-regulated the expression of inflammation-related genes: pro-inflammatory and chemotactic cytokines, including IL-1beta, TNF-alpha, IL-6, CCL2, CCL4, CCL7, CCL24, CXCL1, CXCL2, CXCL5, CXCL9 and CXCL10, and their receptors CCR3 and CCR7, and other colitis-related genes such as COX-2, PAP, MMP family, S100a8, S100a9 and DEFA1. LM treatment inhibited the mRNA expression of inflammation-related and tissue remodeling genes induced by DSS as well as the colitic symptoms. LM inhibition for the DSS-induced expression of the representative inflammatory markers, IL-1beta, TNF-alpha and COX-2, was supported by quantitative real-time polymerase chain reaction analysis. These findings suggest that LM ameliorates DSS-induced colitis by regulating inflammatory-related cytokines as well as tissue remodeling genes.
Lee
Hoyong
H
R & D Center, Korea Yakult Co., Ltd., Yongin, Kyunggi-do 446-901, South Korea.
Ahn
Young-Tae
YT
Lee
Jung-Hee
JH
Huh
Chul-Sung
CS
Kim
Dong-Hyun
DH
D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D003092:Colitis / Q000139:chemically induced / Q000382:microbiology* / Q000473:pathology / Q000517:prevention & control*; D016264:Dextran Sulfate / Q000008:administration & dosage; D020869:Gene Expression Profiling / Q000379:methods; D052196:Levilactobacillus brevis / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D016264:Dextran Sulfate