title: Laser-capture microdissection and protein extraction for protein fingerprint of OSCC and OLK.
abstract: To find new biomarkers and establish histopathology protein fingerprint models for early detection of oral squamous cell carcinoma (OSCC), laser capture microdissection (LCM) technology was utilized in 21 OSCC tissues and 7 oral leukoplaque (OLK) tissues as well as their adjacent normal tissues. Each sample was then detected by SELDI-TOF-MS technology and CM10 protein chip as well as bioinformatics tools. Three proteomic biomarker patterns were identified. Pattern 1 distinguishes patients with OLK from healthy individuals. Pattern 2 distinguishes patients with OSCC from healthy individuals. Pattern 3 distinguishes patients with OSCC from patients with OLK. The analysis yielded both a specificity and a sensitivity of 90.48% for pattern 1, a specificity of 100.00% and a sensitivity of 85.71% for pattern 2, and a specificity of 100.00% and a sensitivity of 85.71% for pattern 3. Proteome mass/charge 3714, 3515, and 4944 built the distinguished protein peaks between the OSCC tumor and adjacent normal tissues. The accuracy of the blind prediction was 90.48% (38/42). M/Z 15122 and 7569 built the distinguished protein peaks between the OLK and adjacent normal tissues. M/Z 3738 and 11366 built the distinguished protein peaks between the OSCC and the OLK. By employing LCM technology combined with SELDI-TOF-MS technology and bioinformatics approaches, histopathology would not only facilitate the discovery of better biomarkers for OSCC and OLK, but also provide a useful tool for molecular diagnosis by potential biomarker.
authors.affiliation: Department of Stomatology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
authors.forename: Feiyun
authors.initials: F
authors.lastname: Ping
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D006416:Hematoxylin / Q000378:metabolism; D006801:Humans; D007053:Ice; D007834:Lasers; D007972:Leukoplakia, Oral / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry; D042282:Microdissection; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D009092:Mucous Membrane / Q000166:cytology / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification*; D040901:Proteomics; D013194:Staining and Labeling
chemical_list: D015415:Biomarkers; D007053:Ice; D011506:Proteins; D006416:Hematoxylin