[Changes of endothelial cell function and platelet activation in rabbit spinal cord with ischemia-reperfusion injury].
OBJECTIVE
To study the changes of vascular endothelial cell function and platelet activation in rabbit spinal cord following ischemia-reperfusion (I/R) injury and their roles in the spinal cord injury.


METHODS
Rabbit spinal cord I/R injury models were established using Zivin method, and the changes in plasma NO and GMP140 levels were dynamically monitored after the injury.


RESULTS
Plasma NO level increased significantly in the I/R group at the end of the ischemia, and reached the peak level at 2 h of reperfusion as compared to that in sham-operated group (P<0.01). Plasma NO level decreased at 6 h of reperfusion, but still significantly higher than the level in the sham-operated group (P<0.05). Plasma GMP140 underwent no significant changes in the sham-operated group, but significantly increased in the I/R group at the end of the ischemia, followed by gradual declination to the normal level at 2 h of reperfusion.


CONCLUSIONS
Spinal cord I/R injury causes overexpressions of NO and GMP140, suggesting the involvement of endothelial cell injury and platelet overactivation in the pathological process and repair of spinal cord I/R injury.
Gong
Shou-ping
SP
Department of Neurosurgery, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. shpinggg@126.com
Wang
Wen-tao
WT
Zhong
Da-lin
DL
Lv
Jian
J
Wu
Feng
F
Che
Jin
J
Seng
Zhi-yuan
ZY
He
Xi-jing
XJ
D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism*; D009569:Nitric Oxide / Q000097:blood; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology* / Q000503:physiopathology*; D013116:Spinal Cord / Q000473:pathology* / Q000503:physiopathology*
D019007:P-Selectin; D009569:Nitric Oxide