Disruption of transepithelial resistance by enterotoxigenic Escherichia coli.
Transepithelial resistance and tight junction protein localization in porcine intestinal epithelial cells were evaluated as a function of infection with porcine enterotoxigenic Escherichia coli isolates differing in adhesin and enterotoxin profiles. Robust heat-labile enterotoxin-independent reduction of host transepithelial resistance was observed in the absence of tight junction protein mislocalization.
Johnson
Amber M
AM
Department of Veterinary Science, South Dakota State University, Brookings, SD 57007, USA.
Kaushik
Radhey S
RS
Hardwidge
Philip R
PR
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D054307:Enterotoxigenic Escherichia coli / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology*; D007422:Intestines / Q000166:cytology / Q000382:microbiology; D008856:Microscopy, Fluorescence; D013552:Swine; D019108:Tight Junctions / Q000382:microbiology