Production and characterization of new families of polyglucuronic acids from TEMPO-NaOCl oxidation of curdlan.
Curdlan from Agrobacterium sp. was oxidized using 2,2,6,6,-tetramethylpiperidine-1-oxyl radical (TEMPO)-NaBr-NaClO systems at pH 11. The effects of oxidation conditions on degrees of oxidation and polymerization of the products obtained were studied using SEC-MALLS, NMR and IR analyses. Different families of water-soluble beta-(1,3)-polyglucuronic and beta-(1,3)-polyglucoglucuronic acid sodium salts were quantitatively generated with a yield of 80% and without significant loss of their molecular weights. Given that beta-(1,3)-polyglucuronic acids prepared from the regioselective oxidation of curdlan by the TEMPO-NaBr-NaClO systems regularly consist of the glucuronic acid repeating unit; they may open new biotechnological fields for the utilizations of water soluble forms of curdlan.
Delattre
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C
GREENTECH Biotechnologies, Biopôle Clermont Limagne 63360 Saint Beauzire, France.
Rios
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L
Laroche
C
C
Le
N H T
NH
Lecerf
D
D
Picton
L
L
Berthon
J Yves
JY
Michaud
P
P
D002241:Carbohydrates / Q000737:chemistry; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000494:pharmacology*; D004563:Electrochemistry / Q000379:methods; D005965:Glucuronates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical; D008970:Molecular Weight; D010100:Oxygen / Q000737:chemistry; D012231:Rhizobium / Q000378:metabolism; D012973:Sodium Hypochlorite / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry*
D002241:Carbohydrates; D003497:Cyclic N-Oxides; D005965:Glucuronates; D047071:beta-Glucans; D014867:Water; C038459:curdlan; D012973:Sodium Hypochlorite; D010100:Oxygen; C003959:TEMPO