title: 9p21 index as estimated by dual-color fluorescence in situ hybridization is useful to predict urothelial carcinoma recurrence in bladder washing cytology.
abstract: Recent studies have shown that chromosome 9p21 locus is frequently deleted in the early stages of urothelial carcinogenesis. To study the predictive value of the 9p21 aberrations in recurrence of urothelial carcinoma of the urinary bladder, we applied dual-color fluorescence in situ hybridization for 9p21 and chromosome 9 centromere to the bladder washing cytology samples that were obtained from the patients with urothelial carcinoma of the urinary bladder treated by transurethral resection. For the evaluation, the 9p21 index was defined as the ratio of the mean number of 9p21 signals per nucleus for that of the chromosome 9 centromere signals per nucleus in each of the bladder washing cytology samples. The 9p21 index values of the bladder washing cytology samples with no (G0) cytologic atypia were significantly higher than those of the bladder washing cytology samples with moderate (G2) (P < .01) and severe (G3) (P < .001) cytologic atypia, but the index values did not statistically differ from those of the bladder washing cytology samples with mild (G1) cytologic atypia. Recurrence-free survival in the patients with a low 9p21 index value (<0.9) was significantly poorer in comparison with the patients with a high 9p21 index value (>0.9). Furthermore, 2 patients of bladder washing cytology G1 with a low 9p21 index value recurred much sooner than the other patients of the bladder washing cytology G1 category. These findings indicate that a decreased 9p21 index value is associated with recurrence of urothelial carcinoma of the urinary bladder, and the 9p21 index may be useful as a marker to identify patients with elevated risk of recurrence of urothelial carcinoma of the urinary bladder.
authors.affiliation: Department of Pathology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan. shig@yamaguchi-u.ac.jp
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kawauchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Sakai
authors.forename: Kenzo
authors.initials: K
authors.lastname: Ikemoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Eguchi
authors.forename: Motonao
authors.initials: M
authors.lastname: Nakao
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Takihara
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shimabukuro
authors.forename: Tomoko
authors.initials: T
authors.lastname: Furuya
authors.forename: Atsunori
authors.initials: A
authors.lastname: Oga
authors.forename: Hideyasu
authors.initials: H
authors.lastname: Matsuyama
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D011237:Predictive Value of Tests; D007507:Therapeutic Irrigation; D001743:Urinary Bladder; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor