The use of MPB70 and MPB83 to distinguish between bovine tuberculosis and paratuberculosis.
In order to demonstrate the potential to distinguish paratuberculosis (PTB) from bovine tuberculosis infection (TB), ELISAs with M. bovis-specific MPB70 or MPB83 as capture antigens were developed and tested on two groups of cattle: Group A comprised 23 animals positive for Mycobacterium avium paratuberculosis (Map) and TB free. Group B comprised 48 animals from a Map free herd during the previous 5 years, but confirmed as tuberculous by positive results on PPD testing and M. bovis culture. Results demonstrated a significant difference (p<0.01) between reactivity of sera from these groups, encouraging the study of purified proteins to differentiate between both diseases.
Marassi
Carla D
CD
Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense, Brazil. carladray@yahoo.com.br
McNair
Jim
J
Pollock
John
J
Ristow
Paula
P
Fonseca
Leila S
LS
Oelemann
Walter M R
WM
Lilenbaum
Walter
W
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D008565:Membrane Proteins / Q000276:immunology*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C094904:MPB83 protein, Mycobacterium; D008565:Membrane Proteins; C060216:MPB70 protein, Mycobacterium bovis