Evidence of inability of human cytomegalovirus to reactivate Kaposi's sarcoma-associated herpesvirus from latency in body cavity-based lymphocytes.
BACKGROUND
Kaposi's sarcoma-associated herpesvirus (KSHV; also known as human herpesvirus 8 (HHV-8)) has been determined to be the most frequent cause of malignancies in AIDS patients. It is associated primarily with Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL), as well as with multicentric Castleman's disease (MCD).(2) The switch from the latent to the lytic stage is important in the maintenance of malignancy and viral infection. So far, the mechanism of its reactivation has not been fully understood.


OBJECTIVE
Human cytomegalovirus (HCMV) and KSHV might infect the same cells, and it was found by other groups that several viruses could reactivate KSHV from latency. We investigate whether HCMV infection could reactivate KSHV from latency in body cavity-based lymphocyte (BCBL-1) cells.


RESULTS
Laboratory strains of HCMV cannot infect B cells. In this article, we demonstrate that the UL131-repaired HCMV (vDW215-BADrUL131) derived from AD169 strain is able to infect B lymphocytes. We directly infected KSHV latent cells including BCBL-1 with vDW215-BADrUL131 to evaluate the ability of HCMV to reactivate KSHV. Inconsistent with previous reports in human fibroblast cells, our results provide direct evidence that HCMV is unable to reactivate KSHV from latency-to-lytic infection in BCBL-1 cell lines. As a control, herpes simplex virus type 1 (HSV-1) was shown to be able to reactivate KSHV.


CONCLUSIONS
Our observations, different from others, suggest that reactivation mechanisms for KSHV might vary in different cells.
Cheng
Bo
B
Department of Dermatology, Fujian Medical University, Fujian, PR China.
Martínez
Francisco Puerta
FP
Katano
Harutaka
H
Tang
Qiyi
Q
D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D018259:Herpesvirus 1, Human / Q000502:physiology; D019288:Herpesvirus 8, Human / Q000254:growth & development / Q000502:physiology*; D006801:Humans; D014775:Virus Activation / Q000502:physiology*; D017735:Virus Latency