title: Sec22b is a negative regulator of phagocytosis in macrophages.
abstract: The endoplasmic reticulum (ER) is proposed to be a membrane donor for phagosome formation. In support of this, we have previously shown that the expression level of syntaxin 18, an ER-localized SNARE protein, correlates with phagocytosis activity. To obtain further insights into the involvement of the ER in phagocytosis we focused on Sec22b, another ER-localized SNARE protein that is also found on phagosomal membranes. In marked contrast to the effects of syntaxin 18, we report here that phagocytosis was nearly abolished in J774 macrophages stably expressing mVenus-tagged Sec22b, without affecting the cell surface expression of the Fc receptor or other membrane proteins related to phagocytosis. Conversely, the capacity of the parental J774 cells for phagocytosis was increased when endogenous Sec22b expression was suppressed. Domain analyses of Sec22b revealed that the R-SNARE motif, a selective domain for forming a SNARE complex with syntaxin18 and/or D12, was responsible for the inhibition of phagocytosis. These results strongly support the ER-mediated phagocytosis model and indicate that Sec22b is a negative regulator of phagocytosis in macrophages, most likely by regulating the level of free syntaxin 18 and/or D12 at the site of phagocytosis.
authors.affiliation: Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hatsuzawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Seisuke
authors.initials: S
authors.lastname: Arai
authors.forename: Taku
authors.initials: T
authors.lastname: Tamura
authors.forename: Arisa
authors.initials: A
authors.lastname: Higa-Nishiyama
authors.forename: Ikuo
authors.initials: I
authors.lastname: Wada
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D009895:Opsonin Proteins / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology*; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D050765:Qa-SNARE Proteins / Q000737:chemistry / Q000502:physiology*; D050767:Qc-SNARE Proteins / Q000737:chemistry / Q000502:physiology*; D050683:R-SNARE Proteins / Q000737:chemistry / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D017452:Receptors, IgG / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000502:physiology; D050600:SNARE Proteins / Q000737:chemistry / Q000502:physiology*; D033921:Vesicular Transport Proteins; D015054:Zymosan / Q000378:metabolism
chemical_list: D009895:Opsonin Proteins; D050765:Qa-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; D034741:RNA, Small Interfering; D017452:Receptors, IgG; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; C432415:Sec22b protein, mouse; C539363:Use1 protein, mouse; D033921:Vesicular Transport Proteins; D015054:Zymosan