[Influence of F protein of hepatitis C virus subtype 1b inhibits on human hepatocellular carcinoma HepG2 cell apoptosis].
OBJECTIVE
To investigate the effects of F protein of hepatitis C virus subtype 1b on the apoptosis of human hepatocellular carcinoma HepG2 cells.


METHODS
HepG2 cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP and pcDNA3.0-F-EGFP-HepG2 strain was exposed to Act-D and tumor necrosis factor alpha (TNF alpha) treatment in order to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-HepG2, negative control pcDNA3.0-C-EGFP-HepG2 and blank control HepG2. Annexin V-FITC/PI of flow cytometry was performed to determine the number of apoptotic cells. DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotic cells.


RESULTS
pcDNA3.0-F-EGFP- HepG2 cell strain showed a much delayed apoptosis as well as obviously lowering the apoptotic rate when compared with the pcDNA3.0-HepG2 strain and HepG2 strain (P < 0.001).


CONCLUSIONS
The introduction and expression of extraneous gene (the F gene of hepatitis C virus subtype 1b) could significantly inhibit the apoptosis of HepG2 cells.
Yang
Jing-jing
JJ
School of Public Health, Nanjing Medical University, Nanjing 210029, China.
Zhang
Yun
Y
Deng
Xiao-zhao
XZ
Xu
Ke
K
Wang
Zhong-can
ZC
Wang
Jie
J
Feng
Le
L
Ding
Wei-liang
WL
D017209:Apoptosis; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005822:Genetic Vectors; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014758:Viral Core Proteins / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha; D014758:Viral Core Proteins; C433031:hepatitis C protein F, Hepatitis C virus