title: Recombinant protein production by a Kaiko-baculovirus system.
abstract: Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the application of a highly efficient and reproducible baculovirus expression system with high throughput using Kaiko (silkworm).
authors.affiliation: Katakura Industries Co., Ltd., Saitama, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Nagaya
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D057166:High-Throughput Screening Assays / Q000379:methods; D008967:Molecular Biology / Q000379:methods; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D014162:Transfection
chemical_list: D011994:Recombinant Proteins