title: Homodimeric alkaline phosphatase located at Helicoverpa armigera midgut, a putative receptor of Cry1Ac contains alpha-GalNAc in terminal glycan structure as interactive epitope.
abstract: Helicoverpa armigera causes massive yield loss of various crops globally. Bacillus thuringiensis coded Cry1Ac toxin is effective against the pest. Through the present investigation, an alkaline phosphatase type putative receptor of the toxin was identified in the target insect gut BBMV. Lectin-ligand immunoblot assay detected the presence of alpha-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction.
authors.affiliation: Plant Molecular and Cellular Genetics, Bose Institute, P-1/12, C.I.T. Scheme, VII-M, Kolkata 700054, India.
authors.forename: Anindya
authors.initials: A
authors.lastname: Sarkar
authors.forename: Daniel
authors.initials: D
authors.lastname: Hess
authors.forename: Hossain A
authors.initials: HA
authors.lastname: Mondal
authors.forename: Santanu
authors.initials: S
authors.lastname: Banerjee
authors.forename: Hari C
authors.initials: HC
authors.lastname: Sharma
authors.forename: Sampa
authors.initials: S
authors.lastname: Das
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D001426:Bacterial Proteins; D002462:Cell Membrane / Q000378:metabolism; D002853:Chromatography, Liquid; D004064:Digestive System / Q000201:enzymology; D004731:Endotoxins / Q000378:metabolism* / Q000633:toxicity; D000939:Epitopes / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D011134:Polysaccharides / Q000378:metabolism*; D055503:Protein Multimerization; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D004731:Endotoxins; D000939:Epitopes; D019476:Insect Proteins; D011134:Polysaccharides; D000469:Alkaline Phosphatase