Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae.
Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100% and correctly identified 246/256 (96.69%) of the positive individuals, while ultrasound detected only 186/256 (73.44%). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick.
Rocha
Abraham
A
Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses e Serviço de Referência em Culicídeos Vetores, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, PE 50670-420, Brasil. rocha@cpqam.fiocruz.br
Braga
Cynthia
C
Belém
Marcela
M
Carrera
Arturo
A
Aguiar-Santos
Ana
A
Oliveira
Paula
P
Texeira
Maria José
MJ
Furtado
André
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood*; D002648:Child; D004605:Elephantiasis, Filarial / Q000097:blood / Q000175:diagnosis* / Q000000981:diagnostic imaging; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008842:Microfilariae / Q000648:ultrastructure*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D014463:Ultrasonography; D014958:Wuchereria bancrofti / Q000276:immunology*; D055815:Young Adult
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth