Sequence and expression analysis of the gene encoding inducible cAMP early repressor in tilapia.
Suppression subtractive hybridization library was generated by comparison of cDNA populations isolated from peripheral leukocytes of pre- and post-immunized tilapia. One cDNA sequence encoding complete inducible cAMP early repressor was obtained from the library. The sequence was characterized by the presence of the basic structure of ICER IIgamma. Expression of ICER was in the tissues of four types of tilapia was decreased after infection with Streptococcus. After immunization, expression of ICER was initially decreased and then increased after 7 days. In addition, the order for the overall expression of ICER gene after infection and the increases of ICER expression later after immunization in these four types of tilapia was positively correlated to the disease resistance and productivity of these four species of tilapia. Our results provided molecular mechanisms for the different disease resistance capability in different species of tilapia. In addition, our results also provided reference molecular marker for breeding disease resistant tilapia, cAMP responsive element modulator.
Chen
Ming
M
Guangxi Institute of Fisheries, 530021 Nanning, Guangxi, People's Republic of China.
Wang
Rui
R
Gan
Xi
X
Lei
Aiying
A
Li
Chao
C
Yu
Xiaoli
X
Huang
Jun
J
Huang
Ting
T
Liang
Wanwen
W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051786:Cyclic AMP Response Element Modulator / Q000737:chemistry / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D008969:Molecular Sequence Data; D012326:RNA Splicing / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000235:genetics; D017210:Tilapia / Q000235:genetics* / Q000382:microbiology
D051786:Cyclic AMP Response Element Modulator