title: siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication.
abstract: A recent RNAi screen used a genome-wide shRNA library to search for cellular factors required for HIV-1 replication. This work complements three other siRNA-based screening studies and potentially opens the door to the discovery of factors that are important for HIV-1 replication in physiological host cells such as T lymphocytes. shRNA screens can be further improved, and they could promise to unravel new pathways and new facets of virus-cell interactions.
authors.affiliation: Department of Biochemistry, University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, PR China. khkok@hkucc.hku.hk
authors.forename: Kin-Hang
authors.initials: KH
authors.lastname: Kok
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Jin
mesh_terms: D002460:Cell Line; D055785:Gene Knockdown Techniques; D015497:HIV-1 / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering; D014779:Virus Replication
chemical_list: D034741:RNA, Small Interfering