title: The use of MPB70 and MPB83 to distinguish between bovine tuberculosis and paratuberculosis.
abstract: In order to demonstrate the potential to distinguish paratuberculosis (PTB) from bovine tuberculosis infection (TB), ELISAs with M. bovis-specific MPB70 or MPB83 as capture antigens were developed and tested on two groups of cattle: Group A comprised 23 animals positive for Mycobacterium avium paratuberculosis (Map) and TB free. Group B comprised 48 animals from a Map free herd during the previous 5 years, but confirmed as tuberculous by positive results on PPD testing and M. bovis culture. Results demonstrated a significant difference (p<0.01) between reactivity of sera from these groups, encouraging the study of purified proteins to differentiate between both diseases.
authors.affiliation: Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense, Brazil. carladray@yahoo.com.br
authors.forename: Carla D
authors.initials: CD
authors.lastname: Marassi
authors.forename: Jim
authors.initials: J
authors.lastname: McNair
authors.forename: John
authors.initials: J
authors.lastname: Pollock
authors.forename: Paula
authors.initials: P
authors.lastname: Ristow
authors.forename: Leila S
authors.initials: LS
authors.lastname: Fonseca
authors.forename: Walter M R
authors.initials: WM
authors.lastname: Oelemann
authors.forename: Walter
authors.initials: W
authors.lastname: Lilenbaum
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D008565:Membrane Proteins / Q000276:immunology*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C094904:MPB83 protein, Mycobacterium; D008565:Membrane Proteins; C060216:MPB70 protein, Mycobacterium bovis