Amino acid substitution at position 464 in the haemagglutinin-neuraminidase protein of a mumps virus Urabe strain enhanced the virus growth in neuroblastoma SH-SY5Y cells.
Mumps virus (MuV) infects various organs including central nervous system (CNS). However, the molecular basis of the neural cell specificity of MuV is not well understood. We found that the Hoshino vaccine strain rescued from cDNA replicated moderately in neuroblastoma SH-SY5Y cell line, while an Urabe strain (Ur89-250) isolated from a post-vaccination aseptic meningitis case replicated efficiently in the same cells. In order to examine the contribution of individual genes of Ur89-250 to the growth in SH-SY5Y cells, recombinant Hoshino vaccine strains in which each gene(s) was replaced with corresponding gene(s) of Ur89-250 were generated. A recombinant virus possessing the small hydrophobic and haemagglutinin-neuraminidase (HN) genes of Ur89-250 grew as efficiently in SH-SY5Y cells as Ur89-250. Further analysis indicated that an amino acid substitution at position 464 in the HN protein was most important for efficient growth. Thus, single amino acid substitution in the HN protein could affect neural cell specificity of mumps virus.
Ninomiya
Kengo
K
Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
Kanayama
Tetsuya
T
Fujieda
Nao
N
Nakayama
Tetsuo
T
Komase
Katsuhiro
K
Nagata
Kyosuke
K
Takeuchi
Kaoru
K
D019943:Amino Acid Substitution; D000818:Animals; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D015707:HN Protein / Q000235:genetics*; D006801:Humans; D009109:Mumps virus / Q000235:genetics / Q000254:growth & development*; D016297:Mutagenesis, Site-Directed; D012367:RNA, Viral / Q000235:genetics; D014709:Vero Cells
D018076:DNA, Complementary; D015707:HN Protein; D012367:RNA, Viral