Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays.
This paper demonstrates a microfluidic chip for multiple immunoassays on electrospun (ES) nanofibrous membranes made of PVDF. This microchip enables the detection of multiple pairs of protein-protein interactions in one experiment by crossing parallel arrays of microchannels. This chip uses ESPVDF membrane with high specific surface area as the substrate to adsorb protein for immobilized immunoassays so that increased levels of protein adsorption may lower the LOD in immunoassays. We employ a simple and effective method for chip assembly by scotch tape to incorporate the ESPVDF membrane into the microchip. We believe that the present work will facilitate the development of methods for the on-site diagnosis of diseases whose detection is based on antigen-antibody recognition, and improve the efficiencies of certain experiments that need to identify tens to hundreds of protein-protein interactions with relatively low cost in the future.
Liu
Yingyi
Y
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, P. R. China.
Yang
Dayong
D
Yu
Tao
T
Jiang
Xingyu
X
D000327:Adsorption; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D019793:Fluorescein / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D009757:Nylons / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000032:analysis; D005465:Fluorocarbon Polymers
D004129:Dimethylpolysiloxanes; D019793:Fluorescein; D008567:Membranes, Artificial; D009757:Nylons; D011145:Polyvinyls; D011506:Proteins; C085707:poly(dimethylsiloxane)-polyamide copolymer; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers