title: The F-BAR domain of srGAP2 induces membrane protrusions required for neuronal migration and morphogenesis.
abstract: During brain development, proper neuronal migration and morphogenesis is critical for the establishment of functional neural circuits. Here we report that srGAP2 negatively regulates neuronal migration and induces neurite outgrowth and branching through the ability of its F-BAR domain to induce filopodia-like membrane protrusions resembling those induced by I-BAR domains in vivo and in vitro. Previous work has suggested that in nonneuronal cells filopodia dynamics decrease the rate of cell migration and the persistence of leading edge protrusions. srGAP2 knockdown reduces leading process branching and increases the rate of neuronal migration in vivo. Overexpression of srGAP2 or its F-BAR domain has the opposite effects, increasing leading process branching and decreasing migration. These results suggest that F-BAR domains are functionally diverse and highlight the functional importance of proteins directly regulating membrane deformation for proper neuronal migration and morphogenesis.
authors.affiliation: Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Sabrice
authors.initials: S
authors.lastname: Guerrier
authors.forename: Jaeda
authors.initials: J
authors.lastname: Coutinho-Budd
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sassa
authors.forename: Aurélie
authors.initials: A
authors.lastname: Gresset
authors.forename: Nicole Vincent
authors.initials: NV
authors.lastname: Jordan
authors.forename: Keng
authors.initials: K
authors.lastname: Chen
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Jin
authors.forename: Adam
authors.initials: A
authors.lastname: Frost
authors.forename: Franck
authors.initials: F
authors.lastname: Polleux
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology; D020690:GTPase-Activating Proteins; D051379:Mice; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology*; D011554:Pseudopodia / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D020690:GTPase-Activating Proteins; C491402:Srgap2 protein, mouse