Patterning of biomolecules on a poly(epsilon-caprolactone) film surface functionalized by ion implantation.
Biomolecule patterning is important due to its potential applications in biodevices, tissue engineering, and drug delivery. In this study, we developed a new method for a biomolecular patterning on poly(epsilon-caprolactone) (PCL) films based on ion implantation. Ion implantation on a PCL film surface resulted in the formation of carboxylic acid groups. The generated carboxylic acid groups were used for the covalent immobilization of amine-functionalized p-DNA, followed by hybridization with fluorescently tagged c-DNA. Biotin-amine was also covalently immobilized on the carboxylic acid generated PCL surfaces. Successful biotin-specific binding of streptavidin further confirmed the potential of this strategy for patterning of various biomolecules.
Hwang
In-Tae
IT
Radiation Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do 580-185, Republic of Korea.
Jung
Chan-Hee
CH
Kim
Dong-Ki
DK
Nho
Young-Chang
YC
Choi
Jae-Hak
JH
D001710:Biotin / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004247:DNA / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D055767:Immobilized Proteins / Q000378:metabolism; D007477:Ions; D046911:Macromolecular Substances / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011091:Polyesters / Q000737:chemistry*; D012834:Silver / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D055767:Immobilized Proteins; D007477:Ions; D046911:Macromolecular Substances; D011091:Polyesters; C016240:polycaprolactone; D012834:Silver; D001710:Biotin; D004247:DNA; D016650:Fluorescein-5-isothiocyanate