Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of epstein-barr virus and cytomegalovirus load.
The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% for EBV and 87.2% for CMV.
Pillet
Sylvie
S
Laboratoire de Bactériologie-Virologie-Hygiène, Hôpital Nord, Centre Hospitalier Universitaire de Saint-Etienne, F-42055 Saint-Etienne Cedex 02, France.
Bourlet
Thomas
T
Pozzetto
Bruno
B
D001331:Automation / Q000379:methods*; D001769:Blood / Q000821:virology*; D003587:Cytomegalovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood* / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D012680:Sensitivity and Specificity; D019562:Viral Load; D014773:Virology / Q000379:methods*
D004279:DNA, Viral