Structural analysis of O-glycans of mucin from jellyfish (Aurelia aurita) containing 2-aminoethylphosphonate.
The structure of O-glycan in qniumucin (Q-mucin), which is a novel mucin extracted from jellyfish, was analyzed by a combination of NMR and ESI-MS/MS. A previously unidentified monosaccharide involved in the glycan chains was determined to be N-acetylgalactosamine (GalNAc) substituted by 2-aminoethylphosphonate (AEP) at the C-6. The O-glycans in Q-mucin from Aurelia aurita were proved to be mainly composed of three monosaccharides: GalNAc, AEP-(O-->6)-GalNAc, and P-6-GalNAc. To the best of our knowledge, this is the first example of an O-glycan structure of glycoproteins containing AEP. This exceptionally simple structure of Q-mucin and its potential use in material science and technology are revealed.
Urai
Makoto
M
Eco-Soft Materials Research Unit, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Nakamura
Takemichi
T
Uzawa
Jun
J
Baba
Takayuki
T
Taniguchi
Kayoko
K
Seki
Hiroko
H
Ushida
Kiminori
K
D000116:Acetylgalactosamine / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D000615:Aminoethylphosphonic Acid / Q000737:chemistry*; D000818:Animals; D009682:Magnetic Resonance Spectroscopy; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D009077:Mucins / Q000737:chemistry* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry*; D037401:Scyphozoa / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D000596:Amino Acids; D009005:Monosaccharides; D009077:Mucins; D011134:Polysaccharides; D000615:Aminoethylphosphonic Acid; D010447:Peptide Hydrolases; D000116:Acetylgalactosamine