title: [Changes of endothelial cell function and platelet activation in rabbit spinal cord with ischemia-reperfusion injury].
abstract: OBJECTIVE
  To study the changes of vascular endothelial cell function and platelet activation in rabbit spinal cord following ischemia-reperfusion (I/R) injury and their roles in the spinal cord injury.
  
  
  METHODS
  Rabbit spinal cord I/R injury models were established using Zivin method, and the changes in plasma NO and GMP140 levels were dynamically monitored after the injury.
  
  
  RESULTS
  Plasma NO level increased significantly in the I/R group at the end of the ischemia, and reached the peak level at 2 h of reperfusion as compared to that in sham-operated group (P<0.01). Plasma NO level decreased at 6 h of reperfusion, but still significantly higher than the level in the sham-operated group (P<0.05). Plasma GMP140 underwent no significant changes in the sham-operated group, but significantly increased in the I/R group at the end of the ischemia, followed by gradual declination to the normal level at 2 h of reperfusion.
  
  
  CONCLUSIONS
  Spinal cord I/R injury causes overexpressions of NO and GMP140, suggesting the involvement of endothelial cell injury and platelet overactivation in the pathological process and repair of spinal cord I/R injury.
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. shpinggg@126.com
authors.forename: Shou-ping
authors.initials: SP
authors.lastname: Gong
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Wang
authors.forename: Da-lin
authors.initials: DL
authors.lastname: Zhong
authors.forename: Jian
authors.initials: J
authors.lastname: Lv
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Jin
authors.initials: J
authors.lastname: Che
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Seng
authors.forename: Xi-jing
authors.initials: XJ
authors.lastname: He
mesh_terms: D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism*; D009569:Nitric Oxide / Q000097:blood; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology* / Q000503:physiopathology*; D013116:Spinal Cord / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D019007:P-Selectin; D009569:Nitric Oxide