The toxic effect of ketamine on SH-SY5Y neuroblastoma cell line and human neuron.
Ketamine used as an injectable anesthetic in human and animal medicine is also a recreational drug used primarily by young adults often at all night dance parties in nightclubs. The percentage of ketamine users has grown very fast in the last 5 years worldwide. However, this leads to the serious question of the long-term adverse effects of ketamine on our nervous system, particularly the brain, because ketamine as an NMDA antagonist could cause neurons to commit apoptosis. Our study therefore aimed to find out the chronic effect of ketamine on neuron using prolonged incubation (48 h) of neuronal cells with ketamine in culture. Our results showed that differentiated neuronal cells were prone to the toxicity of ketamine but probably less susceptible than undifferentiated neuronal cells and fibroblasts. This suggested that the ketamine abuse would be harmful to many other organs as well as the brain. Our results also confirmed that the toxicity of ketamine is related to apoptosis via the Bax/Bcl-2 ratio pathway and caspase-3 in the differentiated neuronal cells. Therefore, long-term ketamine treated cell or animal models should be sought to study this multiorgan effects of ketamine.
Mak
Ying T
YT
School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Lam
Wai P
WP
Lü
Lanhai
L
Wong
Yeuk W
YW
Yew
David T
DT
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D018691:Excitatory Amino Acid Antagonists / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007649:Ketamine / Q000633:toxicity*; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D018691:Excitatory Amino Acid Antagonists; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D007649:Ketamine; D053148:Caspase 3