title: Microbial structure of an anaerobic bioreactor population that continuously dechlorinates 1,2-dichloropropane.
abstract: The bacterial diversity of an anaerobic 1,2-dichloropropane (DCP) dechlorinating bioreactor consortium derived from river sediment has been investigated by a combined molecular approach. By using rDNA clone libraries, denaturing gradient gel electrophoresis and quantitative real-time PCR, both Dehalococcoides ethenogenes- and Dehalobacter restrictus-like 16S rDNA sequences were found within the community. Both species are known for reductive dechlorination of tetrachloroethene. Furthermore, numerous yet-uncultured members of the Green non-sulfur bacteria occurred within the consortium. The community analyses over a period of 14 months revealed a clear population shift. D. restrictus 16S rDNA was enriched significantly and became the most abundant rDNA sequence type, suggesting that Dehalobacter spp. play a key role within the reductive dechlorination of DCP in this consortium. We propose the use of this species as an indicator to monitor the transformation process within the bioreactor.
authors.affiliation: Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Dorotheenstr. 96, 10117 Berlin, Germany.
authors.forename: Cord
authors.initials: C
authors.lastname: Schlötelburg
authors.forename: Claudia
authors.initials: C
authors.lastname: Wintzingerode
authors.forename: Regine
authors.initials: R
authors.lastname: Hauck
authors.forename: Friedrich
authors.initials: F
authors.lastname: Wintzingerode
authors.forename: Werner
authors.initials: W
authors.lastname: Hegemann
authors.forename: Ulf B
authors.initials: UB
authors.lastname: Göbel