[Diagnostic role of BIOMED-2 multiplex polymerase chain reaction for antigen receptor genes rearrangement in lymphoproliferative disorders].
OBJECTIVE
To establish a sensitive and effective method for detection of immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangement,and to explore its role in diagnosis and differential diagnosis of lymphoproliferative disorders.


METHODS
Fifty-eight lymphoid tissue samples from 54 patients with lymphoproliferations were evaluated by the novel BIOMED-2 multiplex polymerase chain reaction (PCR) for antigen receptor genes rearrangement.


RESULTS
Multiplex PCR demonstrated monoclonal Ig/TCR gene rearrangements in 22 of 25 (88.0%) B-cell malignancies and 8 of 15 (53.3%) T-cell malignancies. Among 17 benign lymphoproliferations confirmed histopathologically, polyclonal rearrangements were detected in 14 cases (82.4%). In total, the clonality analysis and the final clinico-histopathological diagnosis were concordant in 77.2%. Combination detection of Iglambda and TCR delta gene rearrangements did not increase the detection rate of monoclonal rearrangement of Ig/TCR, but might help to the detection of Iglambda+ or TCR delta+ lymphomas.


CONCLUSIONS
The novel BIOMED-2 multiplex PCR strategy is a rapid, reliable and sensitive approach to detecting clonality in suspected lymphoproliferations, especially in atypical cases.
Liu
Yan-Ling
YL
Department of Hematology, Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Huang
Mei
M
Li
Deng-Ju
DJ
Xiao
Yi
Y
Tang
Yi
Y
Meng
Fan-Kai
FK
Deng
Jin-Niu
JN
Sun
Han-Ying
HY
Liu
Wen-Li
WL
Zhou
Jian-Feng
JF
D005260:Female; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity