title: Effects of arsenic trioxide on the cerulein-induced AR42J cells and its gene regulation.
abstract: OBJECTIVE
  To study the molecular mechanism of arsenic trioxide-induced cell death on cerulein-stimulated AR42J cells.
  
  
  METHODS
  AR42J cells were incubated for 24 hours, and cerulein (10 nmol/L) and different concentrations of arsenic trioxide were added for another 24 hours. The cells were collected and analyzed for apoptosis and oncosis by using rhodamine 123 and propidium iodide staining, and the changes in the genes that related to cell death were detected by a gene chip.
  
  
  RESULTS
  After cerulein stimulation, apoptosis was significantly increased in the AR42J cells. The addition of arsenic trioxide increased the number of apoptotic cells, and the apoptotic index reached its peak in the 1-micromol/L group. Regarding oncosis, low concentrations of arsenic trioxide (0.5, 1, 2, and 4 micromol/L) reduced the development of oncosis, and in the 2-micromol/L group, it was most significant, whereas high concentration of arsenic trioxide (8 micromol/L) promoted the development of oncosis. A total of 96 genes related to apoptosis were detected, and 36 genes were differentially expressed.
  
  
  CONCLUSIONS
  Appropriate concentrations of arsenic trioxide can induce apoptosis in AR42J cells that were induced by cerulein and lead to changes in the expressions of certain genes.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Harbin Medical University, Chinese Center for Disease Control and Prevention, Harbin Medical University, People's Republic of China.
authors.forename: Dongbo
authors.initials: D
authors.lastname: Xue
authors.forename: Weihui
authors.initials: W
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Liang
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
authors.forename: Bei
authors.initials: B
authors.lastname: Sun
authors.forename: Dianjun
authors.initials: D
authors.lastname: Sun
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D002108:Ceruletide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010087:Oxides / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D002108:Ceruletide; D007770:L-Lactate Dehydrogenase; D000681:Amylases; D000077237:Arsenic Trioxide