Engineering bioluminescent proteins: expanding their analytical potential.
Bioluminescent proteins are used in a plethora of analytical methods, from ultrasensitive assay development to the in vivo imaging of cellular processes. This article reviews the most pertinent current bioluminescent-protein-based technologies and suggests the future direction of this vein of research. (To listen to a podcast about this feature, please go to the Analytical Chemistry multimedia page at pubs.acs.org/page/ancham/audio/index.html .).
Rowe
Laura
L
University of Kentucky, USA.
Dikici
Emre
E
Daunert
Sylvia
S
D000331:Aequorin / Q000737:chemistry; D049454:Firefly Luciferin / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry; D049409:Luciferases, Firefly / Q000737:chemistry / Q000378:metabolism; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D049408:Luminescent Agents; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D000331:Aequorin; D049454:Firefly Luciferin; D049409:Luciferases, Firefly