Rutecarpine ameliorates bodyweight gain through the inhibition of orexigenic neuropeptides NPY and AgRP in mice.
Orexigenic neuropeptides NPY and AgRP play major roles in feeding and are closely related to obesity and diabetic metabolic syndrome. This study explored the inhibitory effect of rutecarpine on feeding and obesity in high-fat-diet-induced (C57BL/6) and leptin-deficient (ob/ob) obese mice. Both mice strains developed obesity, but the obesity was inhibited by the reduced food intake resulting from rutecarpine treatment (0.01%, p<0.01). Blood cholesterol, non-fasting glucose, insulin, and leptin levels were reduced, compared with the control group. Rutecarpine inhibited the expression of NPY and AgRP in the arcuate nucleus (ARC) of the hypothalamus and suppressed the expression of both neuropeptides in N29-4 neuronal cells. These results indicate that rutecarpine ameliorates obesity by inhibiting food intake, which involves inhibited expression of the orexigenic neuropeptides NPY and AgRP.
Kim
Su-Jin
SJ
Jung San Bio Technology, Dukjel-Ri, Jeongnam-Myun, Hwaseong-City, Republic of Korea.
Lee
Sung-Jin
SJ
Lee
Sanghyun
S
Chae
Sungwook
S
Han
Man-Deuk
MD
Mar
Woongchon
W
Nam
Kung-Woo
KW
D054369:Agouti-Related Protein / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D015536:Down-Regulation; D004435:Eating / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D026121:Indole Alkaloids / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009478:Neuropeptide Y / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011799:Quinazolines / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects*; D052999:Quinazolinones
D054369:Agouti-Related Protein; D026121:Indole Alkaloids; D009478:Neuropeptide Y; D011799:Quinazolines; C028632:rutecarpine; D052999:Quinazolinones