Caffeic acid phenethyl ester, an antioxidant from propolis, protects peripheral blood mononuclear cells of competitive cyclists against hyperthermal stress.
Hyperthermal stress and resulting free radical generation is known to impair endurance capacity and immune cell redistribution during prolonged exercise. Caffeic acid phenethyl ester (CAPE), a phenolic compound purified from propolis, has many biological and pharmacological activities including antioxidation. To examine whether CAPE has protective effect against hyperthermal stress in athletes, we isolated peripheral blood mononuclear cells (MNC) from competitive cyclists and assessed their response to hyperthermia with or without CAPE pretreatment. We found that pretreatment of cyclists' MNC with CAPE (0, 1, 2, 4 microg/mL) reversed or reduced hyperthermia-induced survival inhibition, necrosis, superoxide production, glutathione depletion, and intracellular superoxide burst in a dose-dependent manner. These results suggest that CAPE may enhance the hyperthermal tolerance in immune mononuclear cells of competitive cyclists.
Chen
Yu-Jen
YJ
Graduate Inst. of Sport Coaching Science, Chinese Culture Univ., Taipei 111, Taiwan. yu11.tw@yahoo.com.tw
Huang
Ai-Chun
AC
Chang
Hen-Hong
HH
Liao
Hui-Fen
HF
Jiang
Chii-Ming
CM
Lai
Li-Yun
LY
Chan
Jen-Te
JT
Chen
Yu-Yawn
YY
Chiang
Jasson
J
D000975:Antioxidants / Q000494:pharmacology*; D001642:Bicycling; D002109:Caffeic Acids / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005080:Exercise Test; D005978:Glutathione / Q000032:analysis; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009336:Necrosis / Q000517:prevention & control; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D010809:Physical Fitness / Q000502:physiology*; D011429:Propolis / Q000737:chemistry; D013312:Stress, Physiological / Q000187:drug effects*; D013481:Superoxides / Q000032:analysis
D000975:Antioxidants; D002109:Caffeic Acids; D013481:Superoxides; D011429:Propolis; C055494:caffeic acid phenethyl ester; D005978:Glutathione; D010626:Phenylethyl Alcohol