Detection of bluetongue serotype 4 in mouflons (Ovis aries musimon) from Spain.
Bluetongue serotype 4 (BTV4) has been detected for the first time in tissue samples from 2 mouflons (Ovis aries musimon) from the South of Spain, in a retrospective study. The samples included in this study had been fixed and paraffin-embedded for over a year prior to their analysis using a BTV group-specific and a BTV4-specific RT-PCR test. Lung and lymphatic nodes were found positive in both specimens. The amplified DNA was confirmed to be BTV4 by sequencing the RT-PCR products and comparing them with other sequences from GenBank. The combination of RNA extraction from paraffin-embedded samples and serotype-specific real-time RT-PCR assays provides the tools for the detection of BTV from samples stored for a long time. The results shown in this study set out the basis for a greater survey with fixed samples from different species of wild ruminants that the veterinary services have been collecting for years.
Rodríguez-Sánchez
Belén
B
Animal Health Department, Veterinary Faculty, Universidad Complutense de Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain. belenrodriguez@sanidadanimal.info
Sánchez-Cordón
Pedro J
PJ
Molina
Verónica
V
Risalde
María A
MA
Pérez de Diego
Ana Cristina
AC
Gómez-Villamandos
José C
JC
Sánchez-Vizcaíno
José M
JM
D000818:Animals; D001483:Base Sequence; D001819:Bluetongue / Q000473:pathology / Q000821:virology*; D008168:Lung / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D034561:Sheep, Domestic / Q000821:virology*; D013030:Spain