title: Orally administered lactoperoxidase ameliorates dextran sulfate sodium-induced colitis in mice by up-regulating colonic interleukin-10 and maintaining peripheral regulatory T cells.
abstract: We previously demonstrated orally administered bovine lactoperoxidase (LPO) ameliorated dextran sulfate sodium-induced colitis in mice. Here, we examine the mechanism of action of LPO. Three days after colitis induction, expression of interferon-gamma mRNA in colonic tissue was significantly decreased in mice administered LPO; while mRNA expression of interleukin (IL)-10 and regulatory T cell (Treg) marker, Foxp3, were significantly increased. The proportion of CD4+CD25+ Tregs in peripheral CD4+ T cells was also significantly elevated when LPO was administered. Nine days after colitis induction, the severity of colitis symptoms, including body weight loss and colon shortening, was reduced and expression of IL-10 mRNA was increased in mice administered LPO. The proportion of CD4+CD25+ Tregs in peripheral leukocytes was also significantly elevated when LPO was administered. These results suggest LPO ameliorates colitis by up-regulating colonic anti-inflammatory cytokines and maintaining peripheral regulatory T cells.
authors.affiliation: Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., 5-1-83 Higashihara, Zama, Kanagawa 228-8583, Japan. k_shin@morinagamilk.co.jp
authors.forename: Kouichirou
authors.initials: K
authors.lastname: Shin
authors.forename: Ayako
authors.initials: A
authors.lastname: Horigome
authors.forename: Koji
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yaeshima
authors.forename: Keiji
authors.initials: K
authors.lastname: Iwatsuki
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002470:Cell Survival; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D016264:Dextran Sulfate / Q000008:administration & dosage; D018450:Disease Progression; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007784:Lactoperoxidase / Q000008:administration & dosage*; D051379:Mice; D008808:Mice, Inbred CBA; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects; D015431:Weight Loss
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D016753:Interleukin-10; D007371:Interferon-gamma; D016264:Dextran Sulfate; D007784:Lactoperoxidase