Square wave voltammetric detection of Anthrax utilizing a peptide for selective recognition of a protein biomarker.
A short chain peptide (16mer) has been successfully utilized for the selective electrochemical detection of the protein biomarker, protective antigen (PA), for the diagnosis of Anthrax. The major motivation of using a peptide instead of an antibody for the development of a biosensor is that there are advantages associated with the smaller size, better biological stability and easy synthesizability of a peptide. PA-selective peptide was synthesized and conjugated on a binding layer previously immobilized onto gold electrode. The same sensing scheme using a conventional antibody instead of a peptide was also tested for the purpose of comparison. Since the size of the peptide is approximately 1-2 orders of magnitude smaller than that of the antibody, a more populated immobilization of peptide on the sensing layer is possible and will eventually lead to improved sensitivity for PA detection. The selectivity of the peptide-based sensor was evaluated by observing the reduction peaks of samples containing PA with different concentrations of bovine serum albumin (BSA). The resulting responses were interference-free from BSA. The results of this study demonstrate the strong analytical potential of a peptide-based biosensor for diverse applications, especially in disease diagnosis where detection of a specific protein biomarker is particularly demanding.
Huan
Tran Ngoc
TN
Department of Chemistry and Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
Ha
Vu Thi Thu
VT
Hung
Le Quoc
le Q
Yoon
Moon-Young
MY
Han
Sung-Hwan
SH
Chung
Hoeil
H
D000881:Anthrax / Q000175:diagnosis* / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000097:blood*; D001427:Bacterial Toxins / Q000097:blood*; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D010455:Peptides / Q000737:chemistry*; D014159:Transducers
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D015415:Biomarkers; D010455:Peptides; C030325:anthrax toxin