Application of the high-performance liquid chromatography method with coulometric detection for determination of vitamin B(6) in human plasma and serum.
A reversed-phase high-performance liquid chromatography method (HPLC) with coulometric electrochemical detection has been developed and validated for the simultaneous analysis of pyridoxamine-5'-phosphate (PMP), pyridoxamine (PM), pyridoxal 5'-phosphate (PLP), pyridoxal (PL), pyridoxine (PN) and 4-pyridoxic acid (4-PA) in human plasma and serum. The isocratic separation was achieved on C(18) column (250mmx4.6mm, I.D., 5microm) with a mobile phase consisted of 35mM sodium phosphate containing 2.5mM heptanesulfonic acid, adjusted to pH 3.2 with 85% orthophosphoric acid and 12% methanol (v/v). Within-run and between-run precisions expressed by the relative standard deviations were less than 2.7% and 7.7% for all the analysed vitamins and 4-PA, respectively. The limits of detection (LOD) and quantification (LOQ) were: 0.8 and 2.6nM, 1.1 and 3.8nM, 1.5 and 4.5nM, 1.3 and 4.2nM, 1.1 and 3.7nM, 2.1 and 6.3nM for PMP, PM, PLP, PL, PN and 4-PA, respectively. The recoveries ranged from 90.4% to 98.4%. Stability of vitamins was checked under a variety of storage conditions. The developed application demonstrated acceptable sensitivity, precision, accuracy, stability, and linearity over the physiological concentration range. The major advantage of the proposed method is its great simplicity.
Marszałł
Marcin L
ML
Department of Toxicology, Medical University of Gdańsk, Al. Gen. J. Hallera 107, Gdańsk 80-416, Poland. marmartox@amg.gda.pl
Lebiedzińska
Anna
A
Czarnowski
Wojciech
W
Makarowski
Ryszard
R
Kłos
Mateusz
M
Szefer
Piotr
P
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D055664:Electrochemical Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D011735:Pyridoxic Acid / Q000097:blood / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D025101:Vitamin B 6 / Q000097:blood* / Q000378:metabolism
D011735:Pyridoxic Acid; D025101:Vitamin B 6