Fully automated two-dimensional electrophoresis system for high-throughput protein analysis.
A fully automated two-dimensional electrophoresis (2DE) system for rapid and reproducible protein analysis is described. 2DE that is a combination of isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein expression analysis. Here, all the operations are achieved in a shorter time and all the transferring procedures are performed automatically. The system completed the entire process within 1.5 h. A device configuration, operational procedure, and data analysis are described using this system.
Hiratsuka
Atsunori
A
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
Yokoyama
Kenji
K
D000818:Animals; D001331:Automation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D004867:Equipment Design; D005456:Fluorescent Dyes; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008967:Molecular Biology / Q000379:methods; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results
D005456:Fluorescent Dyes; D011506:Proteins