title: Improvement of Bacillus thuringiensis bacteriocin production through culture conditions optimization.
abstract: BUPM4 is a Bacillus thuringiensis subsp. kurstaki strain, isolated from Tunisian soil, producing an original bacteriocin named Bacthuricin F4. The optimization of the latter production conditions was carried out under several physicochemical conditions. It was found that the highest bacteriocin activity was reached at low aeration while bacteriocin synthesis yields were strongly reduced at higher ones. A balance between growth and bacteriocin synthesis, both highly dependent on aeration, was taken into account for the overproduction of bacteriocin. Both glucose and glycerol were shown to be necessary for Bacthuricin F4 maximal synthesis. In addition, the optimal carbon/nitrogen ratio for bacteriocin production is 9. In such optimal conditions, more than 4-fold greater bacteriocin production was obtained than when using TSB medium.
authors.affiliation: Laboratory of Biopesticides, Sfax Biotechnology Centre, Sfax, Tunisia.
authors.forename: Fakher
authors.initials: F
authors.lastname: Kamoun
authors.forename: Nabil
authors.initials: N
authors.lastname: Zouari
authors.forename: Imen
authors.initials: I
authors.lastname: Saadaoui
authors.forename: Samir
authors.initials: S
authors.lastname: Jaoua
mesh_terms: D001413:Bacillus thuringiensis / Q000502:physiology*; D001430:Bacteriocins / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D009584:Nitrogen / Q000378:metabolism*; D011786:Quality Control
chemical_list: D001430:Bacteriocins; D002244:Carbon; D009584:Nitrogen