Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.
Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.
Espinosa-Jeffrey
Araceli
A
David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Wakeman
Dustin R
DR
Kim
Seung U
SU
Snyder
Evan Y
EY
de Vellis
Jean
J
D000818:Animals; D000831:Animals, Newborn; D001681:Biological Assay; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D015925:Cryopreservation; D006801:Humans; D009186:Myelin Sheath / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology
D019006:Neural Cell Adhesion Molecules