title: Comparative methodologies for measuring metabolizable energy of various types of resistant high amylose corn starch.
abstract: Energy values of high amylose corn starches high in resistant starch (RS) were determined in vivo by two different methodologies. In one study, energy values were determined according to growth relative to glucose-based diets in rats fed diets containing RS(2), heat-treated RS(2) (RS(2)-HT), RS(3), and amylase predigested versions to isolate the RS component. Net metabolizable energy values ranged from 2.68 to 3.06 kcal/g for the RS starches, and 1.91-2.53 kcal/g for the amylase predigested versions. In a second study, rats were fed a diet containing RS(2)-HT and the metabolizable energy value was determined by bomb calorimetry. The metabolizable energy value was 2.80 kcal/g, consistent with Study 1. Thus, high amylose corn based RS ingredients and their amylase predigested equivalents have energy values approximately 65-78% and 47-62% of available starch (Atwater factor), respectively, according to the RS type (Garcia, T. A.; McCutcheon, K. L.; Francis, A. R.; Keenan, M. J.; O'Neil, C. E.; Martin, R. J.; Hegsted, M. The effects of resistant starch on gastrointestinal organs and fecal output in rats. FASEB J. 2003, 17, A335).
authors.affiliation: Division of Human Nutrition and Food, School of Human Ecology, Louisiana State University AgCenter, Baton Rouge, Louisiana 70803, USA. rtulley@agcenter.lsu.edu
authors.forename: Richard T
authors.initials: RT
authors.lastname: Tulley
authors.forename: Marko J
authors.initials: MJ
authors.lastname: Appel
authors.forename: Tanya G
authors.initials: TG
authors.lastname: Enos
authors.forename: Maren
authors.initials: M
authors.lastname: Hegsted
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: McCutcheon
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Anne M
authors.initials: AM
authors.lastname: Raggio
authors.forename: Roger
authors.initials: R
authors.lastname: Jeffcoat
authors.forename: Anne
authors.initials: A
authors.lastname: Birkett
authors.forename: Roy J
authors.initials: RJ
authors.lastname: Martin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keenan
mesh_terms: D000681:Amylases / Q000378:metabolism; D000688:Amylose / Q000032:analysis*; D000818:Animals; D002151:Calorimetry; D004032:Diet; D004063:Digestion; D002149:Energy Intake; D004734:Energy Metabolism; D005243:Feces / Q000737:chemistry; D006358:Hot Temperature; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013213:Starch / Q000737:chemistry* / Q000378:metabolism*; D014556:Urine / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*
chemical_list: D013213:Starch; D000688:Amylose; D000681:Amylases