A beta-D-allopyranoside-grafted Ru(II) complex: synthesis and acid-base and DNA-binding properties.
A new ruthenium(II) complex grafted with beta-d-allopyranoside, Ru(bpy)(2)(Happip)(ClO(4))(2) (where bpy = 2,2'-bipyridine; Happip = 2-(4-(beta-d-allopyranoside)phenyl)imidazo[4,5-f][1,10]phenanthroline), has been synthesized and characterized by elemental analysis, (1)H NMR spectroscopy, and mass spectrometry. The acid-base properties of the complex have been studied by UV-visible and luminescence spectrophotometric pH titrations, and ground- and excited-state ionization constants have been derived. The Ru(II) complex functions as a DNA intercalator as revealed by UV-visible and emission titrations, salt effects, steady-state emission quenching by [Fe(CN)(6)](4-), DNA competitive binding with ethidium bromide, DNA melting experiment, and viscosity measurements.
Ma
Yan-Zi
YZ
College of Chemistry and Key Laboratory of Radiopharmaceuticals, Ministry of Education, Beijing Normal University, Beijing 100875, People's Republic of China.
Yin
Hong-Ju
HJ
Wang
Ke-Zhi
KZ
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004996:Ethidium / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006027:Glycosides / Q000138:chemical synthesis / Q000737:chemistry*; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D013054:Spectrophotometry, Atomic; D014783:Viscosity
D005456:Fluorescent Dyes; D006027:Glycosides; D007364:Intercalating Agents; D009942:Organometallic Compounds; C103599:bis(bipyridyl)ruthenium(II); D015082:2,2'-Dipyridyl; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium