H2O2/HCl and heat-treated Ti-6Al-4V stimulates pre-osteoblast proliferation and differentiation.
The purpose of the present study was to evaluate the bioactivity of chemical treatment of titanium alloy (Ti-6Al-4V) in vitro. Smooth-surface discs of Ti-6Al-4V were used in this study. Sandblasted, dual acid-etched and H(2)O(2)/HCl heat-treated discs were set as test group, and sandblasted, dual acid-etched discs as control group. SEM and XRD analysis revealed a porous anatase gel layer on rough surface in the test group and a rough surface in the control group. Mouse pre-osteoblasts (MC3T3-E1 cells) were cultured on these 2 group discs, and then cell proliferation and differentiation were examined 4 days, 7 days, and 14 days after cell seeding. Cell proliferation was greatly stimulated at all time points when cultured in test group (P < .05). The alkaline phosphatase (ALP) activity and osteocalcin (OC) production were much higher in the test group compared with the control group at every time point investigated (P < .05). Furthermore, in the test group, the expressions of alkaline phosphatase-2, osteocalcin, and collagen type I alpha 1 mRNAs were significantly up-regulated as compared with those in the control group (P < .05 or P < .01). The results suggested that H(2)O(2)/HCl and heat-treatment might facilitate better integration of Ti-6Al-4V implants with bone.
Shi
Geng-sheng
GS
Stomatology Center of Taizhou Hospital, Zhejiang Province, Linhai, P. R. China. painfulzf@yahoo.cn
Ren
Ling-fei
LF
Wang
Lin-zhi
LZ
Lin
Hai-sheng
HS
Wang
Sha-bin
SB
Tong
Yong-qing
YQ
D016475:3T3 Cells; D000134:Acid Etching, Dental / Q000379:methods*; D000199:Actins / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D003722:Dental Alloys / Q000737:chemistry*; D045662:Dental Etching / Q000379:methods; D006851:Hydrochloric Acid / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000032:analysis; D016877:Oxidants / Q000737:chemistry*; D016062:Porosity; D017655:Silicon Compounds / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D015854:Up-Regulation; D014961:X-Ray Diffraction
D000199:Actins; D000497:Alloys; D001672:Biocompatible Materials; D015415:Biomarkers; D017554:Carbon Compounds, Inorganic; D003722:Dental Alloys; D016877:Oxidants; D017655:Silicon Compounds; D015675:Osteocalcin; C031462:titanium alloy (TiAl6V4); C009495:titanium dioxide; D006861:Hydrogen Peroxide; D014025:Titanium; C510878:ALPL protein, mouse; D000469:Alkaline Phosphatase; D006851:Hydrochloric Acid; C022088:silicon carbide