[Mitochondrial DNA D-loop variation types in Tibet mini-pigs in association with the blood parameters].
OBJECTIVE
To analyze the mitochondrial DNA (mtDNA) D-loop region sequence variation in Tibet Mini-Pigs in relation to the blood parameters and provide the molecular genetic basis for developing new species of laboratory animals.


METHODS
The genomic DNA was extracted from the whole blood samples of 59 Tibet mini-pigs to amplifying the mtDNA D-loop for sequence analysis. Nine physiological and nine biochemical blood parameters of Tibet mini-pigs were measured .


RESULTS
Based on the variation of the tandem repeat motif, the mtDNA D-loop region of Tibet mini-pigs was classified into two types, namely type A and B with the percentage of 57.6% and 42.4%, respectively, roughly matching the 3 transform sites (305, 500, 691) at the 5' end. In the 18 blood parameters, only red blood cell count showed significant differences between types A and (P<0.01).


CONCLUSIONS
Based on the sequence variation of the mtDNA D-loop region, Tibet mini-pigs can be divided into two types that show a significant difference in red blood cell count.
Li
Hong-tao
HT
Laboratory Animal Center, Southern Medical University, Guangzhou 510515, China. hongtao@zzu.edu.cn
Wu
Qing-hong
QH
Yuan
Jin
J
Xiao
Dong
D
Wang
Wan-shan
WS
Zhang
Jia-ning
JN
Zhang
Jian-ming
JM
Li
Jin-ze
JZ
Gu
Wei-wang
WW
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D006403:Hematologic Tests; D016133:Polymerase Chain Reaction; D013552:Swine / Q000097:blood* / Q000235:genetics*; D018609:Tibet
D004272:DNA, Mitochondrial