title: Biopolymer from microbial assisted in situ hydrolysis of triglycerides and dimerization of fatty acids.
abstract: The present study demonstrates biopolymer production by in situ bio-based dimerization of fatty acids by microorganism isolated from marine sediments. Microbial isolate grown in Zobell medium in the presence of triglycerides for the period of 24-240 h at 37 degrees C, hydrolyze the applied triglycerides and sequentially dimerized the hydrolyzed products and subsequently polymerized and transformed to a biopolymer having appreciable adhesive properties. Physical (nature, odour, stickyness and tensile strength), chemical (instrumentation) and biochemical (cell free broth) methods of analyses carried out provided the hypotheses involved in the formation of the product as well as the nature of the product formed. Results revealed, lipolytic enzymes released during initial period of growth and the biosurfactant production during later period, respectively, hydrolyze the applied triglycerides and initiate the dimerization and further accelerated when the incubation period extended. The existence and the non-existence of in situ hydrolysis of various triglycerides followed by dimerization and polymerization and the mechanism of transformation of triglycerides to biopolymer are discussed in detail.
authors.affiliation: Microbiology Division, Central Leather Research Institute, Adyar (Council of Scientific and Industrial Research, New Delhi), Chennai 20, Tamil Nadu, India.
authors.forename: V
authors.initials: V
authors.lastname: Kavitha
authors.forename: N
authors.initials: N
authors.lastname: Radhakrishnan
authors.forename: E
authors.initials: E
authors.lastname: Madhavacharyulu
authors.forename: G
authors.initials: G
authors.lastname: Sailakshmi
authors.forename: G
authors.initials: G
authors.lastname: Sekaran
authors.forename: B S R
authors.initials: BS
authors.lastname: Reddy
authors.forename: G Suseela
authors.initials: GS
authors.lastname: Rajkumar
authors.forename: Arumugam
authors.initials: A
authors.lastname: Gnanamani
mesh_terms: D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D006868:Hydrolysis; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism*; D013718:Tensile Strength; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*; D014783:Viscosity
chemical_list: D001704:Biopolymers; D005227:Fatty Acids; D013501:Surface-Active Agents; D014280:Triglycerides