title: The genotype-phenotype relationship in multicellular pattern-generating models--the neglected role of pattern descriptors.
abstract: BACKGROUND
  A deep understanding of what causes the phenotypic variation arising from biological patterning processes, cannot be claimed before we are able to recreate this variation by mathematical models capable of generating genotype-phenotype maps in a causally cohesive way. However, the concept of pattern in a multicellular context implies that what matters is not the state of every single cell, but certain emergent qualities of the total cell aggregate. Thus, in order to set up a genotype-phenotype map in such a spatiotemporal pattern setting one is actually forced to establish new pattern descriptors and derive their relations to parameters of the original model. A pattern descriptor is a variable that describes and quantifies a certain qualitative feature of the pattern, for example the degree to which certain macroscopic structures are present. There is today no general procedure for how to relate a set of patterns and their characteristic features to the functional relationships, parameter values and initial values of an original pattern-generating model. Here we present a new, generic approach for explorative analysis of complex patterning models which focuses on the essential pattern features and their relations to the model parameters. The approach is illustrated on an existing model for Delta-Notch lateral inhibition over a two-dimensional lattice.
  
  
  RESULTS
  By combining computer simulations according to a succession of statistical experimental designs, computer graphics, automatic image analysis, human sensory descriptive analysis and multivariate data modelling, we derive a pattern descriptor model of those macroscopic, emergent aspects of the patterns that we consider of interest. The pattern descriptor model relates the values of the new, dedicated pattern descriptors to the parameter values of the original model, for example by predicting the parameter values leading to particular patterns, and provides insights that would have been hard to obtain by traditional methods.
  
  
  CONCLUSIONS
  The results suggest that our approach may qualify as a general procedure for how to discover and relate relevant features and characteristics of emergent patterns to the functional relationships, parameter values and initial values of an underlying pattern-generating mathematical model.
authors.affiliation: Centre for Integrative Genetics (CIGENE), Norwegian University of Life Sciences, N-1432 As, Norway. harald.martens@umb.no
authors.forename: Harald
authors.initials: H
authors.lastname: Martens
authors.forename: Siren R
authors.initials: SR
authors.lastname: Veflingstad
authors.forename: Erik
authors.initials: E
authors.lastname: Plahte
authors.forename: Magni
authors.initials: M
authors.lastname: Martens
authors.forename: Dominique
authors.initials: D
authors.lastname: Bertrand
authors.forename: Stig W
authors.initials: SW
authors.lastname: Omholt
mesh_terms: D000818:Animals; D002468:Cell Physiological Phenomena; D003198:Computer Simulation; D005838:Genotype; D006801:Humans; D008954:Models, Biological; D009024:Morphogenesis / Q000502:physiology*; D010641:Phenotype