Enzymatic and cellular responses in relation to body burden of PAHs in bivalve molluscs: a case study with chronic levels of North Sea and Barents Sea dispersed oil.
Mytilus edulis and Chlamys islandica were exposed to nominal dispersed crude oil concentrations in the range 0.015-0.25 mg/l for one month. Five biomarkers (enzymatic and cellular responses) were analysed together with bioaccumulation of PAHs at the end of exposure. In both species, PAH tissue residues reflected the exposure concentration measured in the water and lipophilicity determined the bioaccumulation levels. Oil caused biomarker responses in both species but more significant alterations in exposed C. islandica were observed. The relationships between exposure levels and enzymatic responses were apparently complex. The integrated biomarker response related against the exposure levels was U-shaped in both species and no correlation with total PAH body burden was found. For the monitoring of chronic offshore discharges, dose- and time-related events should be evaluated in the selection of biomarkers to apply. From this study, cellular damages appear more fitted than enzymatic responses, transient and more complex to interpret.
Baussant
T
T
International Research Institute of Stavanger/IRIS-Biomiljø, Mekjarvik 12, 4070 Randaberg, Norway. thierry.baussant@iris.no
Bechmann
R K
RK
Taban
I C
IC
Larsen
B K
BK
Tandberg
A H
AH
Bjørnstad
A
A
Torgrimsen
S
S
Naevdal
A
A
Øysaed
K B
KB
Jonsson
G
G
Sanni
S
S
D000818:Animals; D001267:Atlantic Ocean; D015415:Biomarkers / Q000378:metabolism; D001822:Body Burden; D002374:Catalase / Q000378:metabolism; D004784:Environmental Monitoring; D005982:Glutathione Transferase / Q000378:metabolism; D049880:Mytilus edulis / Q000187:drug effects* / Q000201:enzymology; D009659:North Sea; D049897:Pectinidae / Q000187:drug effects* / Q000201:enzymology; D010578:Petroleum / Q000378:metabolism / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D012623:Seawater / Q000737:chemistry; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*
D015415:Biomarkers; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D002374:Catalase; D005982:Glutathione Transferase