Plant micrometabolomics: the analysis of endogenous metabolites present in a plant cell or tissue.
Identification and quantification of metabolites occurring within specific cell types or single cells of plants and other organisms is of particular interest for natural product chemistry, chemical ecology, and biochemistry in general. The integration of studies at the gene, transcript, protein and metabolite levels in localized regions will provide useful information for the understanding of biology as a system. In this review, we summarize the latest developments in the analysis of metabolites present in small samples, micrometabolomics, dealing with sample preparation methods, with focus on laser-assisted microdissection, and the analytical technologies used. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) are among the most emergent technologies in metabolomics, enabling the shortest route toward metabolite identification.
Moco
Sofia
S
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, Germany. sofia.moco@wur.nl
Schneider
Bernd
B
Vervoort
Jacques
J
D022082:Cellular Structures / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055432:Metabolomics; D042282:Microdissection / Q000379:methods; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D010940:Plant Proteins