[Identification of differentially expressed genes in myocardium of patients with heart failure by human whole genomic oligonucleotide microarray-assisted pathways analysis].
OBJECTIVE
To identify the differentially expressed gene profiles in myocardium of patients with heart failure using human whole genomic oligonucleotide microarray-assisted pathway analysis.


METHODS
Phalanx whole genomic oligonucleotide microarrays were used to detect the gene expression profiles of myocardium in four patients died of heart failure and 4 brain died patients without heart diseases. The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The genes with a threshold of 1.2 times fold-change were selected and BioCarta Pathway and KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway databases were used to identify functionally related gene pathways.


RESULTS
A total of 2806 genes with differentially expression were detected between the failing and non-failing heart samples, expression changes of 399 genes were more than 2-folds. Eleven pathways were identified by BioCarta pathway database and sixteen pathways were identified by KEGG PATHWAY Database.


CONCLUSIONS
Genomic microarray-assisted pathway analysis could help to identify gene expression profiles in failing heart.
Wu
Xiao-Xia
XX
Department of Cardiology, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
Wan
Tao
T
Wu
Hong-Jin
HJ
Zhi
Guang
G
Xiao
Cang-Song
CS
Gao
Chang-Qing
CQ
Wu
Jia-Jin
JJ
D000328:Adult; D000368:Aged; D005260:Female; D020869:Gene Expression Profiling; D005796:Genes; D015894:Genome, Human; D055106:Genome-Wide Association Study; D005838:Genotype; D006333:Heart Failure / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
D012313:RNA