Methoxyl groups of lignin are essential carbon donors in C1 metabolism of Sphingobium sp. SYK-6.
Sphingobium sp. SYK-6 can utilize lignin biphenyl compounds, such as 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl (DDVA). In the metabolism of DDVA, this microorganism exploits two O -demethylation systems with different substrate specificities, namely, a DDVA-specific oxygenative O -demethylation system and a vanillate-specific (VA-specific) tetrahydrofolate-dependent (THF-dependent) methyltransferase system. We examined the way in which these two systems interact in the metabolism of lignin in Sphingobium sp. SYK-6. Our results indicate that THF accepts methyl groups derived not only from the THF-dependent O -demethylation of VA but also from the oxygenative O -demethylation of DDVA. Thus, the methoxyl groups of lignin model compounds are an essential source of carbon in Sphingobium sp. SYK-6. ((c) 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
Sonoki
Tomonori
T
Faculty of Agriculture and Life Sciences, Hirosaki University, Bunkyo-cho, Hirosaki, Aomori, Japan.
Masai
Eiji
E
Sato
Kanna
K
Kajita
Shinya
S
Katayama
Yoshihiro
Y
D002244:Carbon / Q000378:metabolism*; D002247:Carbon Isotopes; D005798:Genes, Bacterial; D008031:Lignin / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism; D042301:Sphingomonadaceae / Q000378:metabolism*; D013379:Substrate Specificity; D013763:Tetrahydrofolates / Q000378:metabolism; D014641:Vanillic Acid / Q000378:metabolism
D002247:Carbon Isotopes; D013763:Tetrahydrofolates; D002244:Carbon; D008031:Lignin; D008780:Methyltransferases; D014641:Vanillic Acid