Refractometry of melanocyte cell nuclei using optical scatter images recorded by digital Fourier microscopy.
The cell nucleus is the dominant optical scatterer in the cell. Neoplastic cells are characterized by cell nucleus polymorphism and polychromism-i.e., the nuclei exhibits an increase in the distribution of both size and refractive index. The relative size parameter, and its distribution, is proportional to the product of the nucleus size and its relative refractive index and is a useful discriminant between normal and abnormal (cancerous) cells. We demonstrate a recently introduced holographic technique, digital Fourier microscopy (DFM), to provide a sensitive measure of this relative size parameter. Fourier holograms were recorded and optical scatter of individual scatterers were extracted and modeled with Mie theory to determine the relative size parameter. The relative size parameter of individual melanocyte cell nuclei were found to be 16.5+/-0.2, which gives a cell nucleus refractive index of 1.38+/-0.01 and is in good agreement with previously reported data. The relative size parameters of individual malignant melanocyte cell nuclei are expected to be greater than 16.5.
Seet
Katrina Y T
KY
The University of Queensland, Centre for Biophotonics and Laser Science, School of Physical Sciences and School of Information Technology and Electrical Engineering, St. Lucia, Queensland, 4072 Australia. katseet@gmail.com
Nieminen
Timo A
TA
Zvyagin
Andrei V
AV
D000465:Algorithms; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D005583:Fourier Analysis; D006696:Holography / Q000379:methods*; D006801:Humans; D008544:Melanocytes / Q000166:cytology*; D008853:Microscopy / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D012815:Signal Processing, Computer-Assisted