Glucoamylase by recombinant Kluyveromyces lactis cells: production and modelling of a fed batch bioreactor.
A cultural system, aimed at the production of glucoamylase with cells of a non-conventional yeast transformed for the enzyme expression, Kluyveromyces lactis JA6-GAA was realised. Glucoamylase production was accomplished in a reactor operating in fed batch mode to avoid limitations with respect to oxygen transfer, and achieve high cell density. A mathematical model able to describe batch and fed batch operations was developed. The theoretical and experimental approach permitted to catch sight of possible physiological changes in the producer strain and set up a suitable fed-batch run to achieve a higher cell density.
Paciello
Lucia
L
Department of Ingegneria Chimica e Alimentare, Università degli Studi di Salerno, Fisciano, Italy.
Romano
Franca
F
de Alteriis
Elisabetta
E
Parascandola
Palma
P
Romano
Vittorio
V
D057005:Bioengineering; D019149:Bioreactors / Q000382:microbiology*; D015169:Colony Count, Microbial; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007700:Kinetics; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D007785:Lactose / Q000378:metabolism; D008954:Models, Biological; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D011994:Recombinant Proteins; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose; D007785:Lactose; D005990:Glycerol