Stability of N-glycan profiles in human plasma.
Glycan heterogeneity was shown to be associated with numerous diseases and glycan analysis has a great diagnostic potential. Recently, we reported high biological variability of human plasma N-glycome at the level of population. The observed variations were larger than changes reported to be associated with some diseases; thus, it was of great importance to examine the temporal constancy of human N-glycome before glycosylation changes could be routinely analyzed in diagnostic laboratories. Plasma samples were taken from 12 healthy individuals. The blood was drawn on seven occasions during 5 days. N-Linked glycans, released from plasma proteins, were separated using hydrophilic interaction high-performance liquid chromatography into 16 groups (GP1-GP16) and quantified. The results showed very small variation in all glycan groups, indicating very good temporal stability of N-glycome in a single individual. Coefficients of variation from 1.6% for GP8 to 11.4% for GP1 were observed. The average coefficient of variation was 5.6%. These variations were comparable to those observed when analytical procedure was tested for its precision. Good stability of plasma N-glycome in healthy individuals implies that glycosylation is under significant genetic control. Changes observed in glycan profiles are consequence of environmental influences and physiologic responses and therefore have a significant diagnostic potential.
Gornik
Olga
O
University of Zagreb, Faculty of Pharmacy and Biochemistry, Ante Kovacića 1, 10000 Zagreb, Croatia.
Wagner
Jasenka
J
Pucić
Maja
M
Knezević
Ana
A
Redzic
Irma
I
Lauc
Gordan
G
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism / Q000502:physiology; D001774:Blood Chemical Analysis; D001798:Blood Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005260:Female; D054794:Glycomics / Q000379:methods; D006031:Glycosylation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000097:blood* / Q000378:metabolism*; D013997:Time Factors
D001798:Blood Proteins; D011134:Polysaccharides