title: [Identification of human testicular embryonal carcinoma proteins by two-dimensional gel electrophoresis and mass spectrometry].
abstract: OBJECTIVE
  To separate and identify human testicular embryonal carcinoma proteomics using two-dimensional electrophoresis (2-DE) and mass spectrometry.
  
  
  METHODS
  Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of the samples. After silver staining, PDQuest 7.30 image analysis software was applied to analyze the 2-DE images. Three of the proteins highly expressed in human testicular embryonal carcinoma were identified by matrix-assisted laser adsorption/ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS).
  
  
  RESULTS
  2-DE effectively screened the differentially expressed proteins in the carcinoma tissues. Three proteins highly expressed in the carcinoma were successfully identified.
  
  
  CONCLUSIONS
  The proteins of human testicular embryonal carcinoma can be effectively separated and analyzed using 2-DE and mass spectrometry. Proteomic analysis offers a new means for further study of this carcinoma.
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Ya-guang
authors.initials: YG
authors.lastname: Zou
authors.forename: Qi-zhao
authors.initials: QZ
authors.lastname: Zhou
authors.forename: Tie-qiu
authors.initials: TQ
authors.lastname: Li
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Guo
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Jing
authors.forename: Xiang-ming
authors.initials: XM
authors.lastname: Mao
authors.forename: Wan-long
authors.initials: WL
authors.lastname: Tan
authors.forename: Shao-bin
authors.initials: SB
authors.lastname: Zheng
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D018236:Carcinoma, Embryonal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor