title: Development of a sensitive high-performance liquid chromatographic method with simple extraction for simultaneous determination of huperzine A and huperzine B in the species containing lycopodium alkaloids.
abstract: A sensitive HPLC method with simple extraction was developed for simultaneous determination of huperzine A (HupA) and huperzine B (HupB) in Huperzia serrata, H. crispata, H. miyoshiana, and Lycopodiastrum casuarinoides. In order to avoid conventional multiple-step and time-consuming sample preparation methods, direct reflux extraction with alkaline chloroform was adopted. The quantitative determination was conducted by reversed-phase HPLC with a photodiode array detector set at 308 nm. Separation was performed on a Luna C18 column (250 x 4.6 mm id, 5 microm) with methanol-0.2% aqueous acetic acid (18 + 82, v/v) mobile phase. The method was validated for accuracy, reproducibility, precision, and limits of detection and quantification. Quantification of the two active compounds in the samples was performed by this newly developed method, and the content of HupA and HupB varied substantially among four different species. The satisfactory results indicated that the developed method can readily be utilized for quality control of the species of Huperziaceae and Lycopodiaceae containing the two compounds.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100191, People's Republic of China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Junbo
authors.initials: J
authors.lastname: Xie
authors.forename: Wen-Qian
authors.initials: WQ
authors.lastname: Chen
authors.forename: Tian-Yan
authors.initials: TY
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D032501:Huperzia / Q000737:chemistry*; D007202:Indicators and Reagents; D032502:Lycopodium / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000032:analysis*; D012996:Solutions
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D007202:Indicators and Reagents; D010936:Plant Extracts; D012717:Sesquiterpenes; D012996:Solutions; C050426:huperzine A; C053016:huperzine B