Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera.
Effects of 18 commercial lots of fetal calf serum (FCS) after gamma-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P<0.05-0.001, Student's t-test) and 61.1% (P<0.05-0.001, chi(2) test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P<0.05-0.001, chi(2) test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
Pilili
Juan Pablo
JP
Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina.
González
Norma V
NV
Molinari
Gabriela
G
Reigosa
Miguel A
MA
Soloneski
Sonia
S
Larramendy
Marcelo L
ML
D000818:Animals; D017848:Blood-Borne Pathogens / Q000528:radiation effects; D016466:CHO Cells; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003132:Commerce; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000494:pharmacology; D001908:Diarrhea Viruses, Bovine Viral / Q000528:radiation effects; D004285:Dogs; D005312:Fetal Blood / Q000502:physiology* / Q000528:radiation effects; D009152:Mutagenicity Tests; D044967:Serum / Q000502:physiology / Q000528:radiation effects; D013242:Sterilization / Q000379:methods; D018675:Toxicity Tests / Q000379:methods*
D003470:Culture Media