[Effects of FAK expression level on proliferation and motility of colorectal carcinoma cells].
OBJECTIVE
To investigate the effect of siRNA targeting FAK gene on proliferation and motility of colorectal cancer.


METHODS
Recombinant plasmids that produced siRNAs targeting FAK were designed and cloned, then transfected into Caco-2 cells. The changes of FAK expression levels were examined by RT-PCR and immunocytochemistry. The effects of FAK gene knockdown on apoptotic morphological changes, proliferation, and motility were investigated.


RESULTS
Recombinant plasmids targeting FAK were successfully constructed, FAK mRNA and protein level was silenced in Caco-2 cells significantly. The inhibition of mRNA level was achieved maximal at 48 h post transfection with time dependent. The ability of proliferation and motility of Caco-2 cells were significantly decreased.


CONCLUSIONS
Plasmid-mediated FAK siRNA could inhibit FAK gene expression. The proliferation, motility and apoptosis were inhibited effectively, which suggested that FAK expression is closely associated with the proliferation, motility and apoptosis, etc. The results may be used as the reference for gene therapy of colorectal cancer.
Pan
Ning
N
Lab of Tumor Cellular and Molecular Biology, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China. pnpanpan07@126.com
Zhang
Ai-ran
AR
Mu
Mao-sen
MS
Hou
Ying-chun
YC
D017209:Apoptosis; D018938:Caco-2 Cells; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D051417:Focal Adhesion Kinase 1 / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
D034741:RNA, Small Interfering; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human