Electrophoretic total analysis of trace tetracycline antibiotics in a microchip with amperometry.
The preconcentration, separation, and electrochemical detection of a series of tetracycline (TC) antibiotics in a microfluidic channel were preformed. The electrophoretic experimental parameters to analyze TC, oxytetracycline, chlortetracycline, and doxycycline antibiotics were investigated with a cellulose-dsDNA-modified carbon paste electrode. Modification of the electrode improved detection performance by enhancing the signal-to-noise characteristics without surface fouling of the electrode. Field-amplified sample stacking and field-amplified sample injection techniques were employed for on-chip preconcentration of the TC series. The sensitivity of the method was improved 10 900-fold when compared with conventional MEKC-electrochemical detection analysis. The overall recoveries for TC, oxytetracycline, chlortetracycline, and doxycycline were 87, 89, 87, and 81%, respectively, with 6.0% RSD. The limits of detection for the series were estimated between 1.5 and 4.3 nM. Applicability of the method to beef samples was successfully demonstrated.
Lee
Kyung-Sun
KS
Department of Chemistry, Pusan National University, Busan, South Korea.
Park
So-Hee
SH
Won
So-Young
SY
Shim
Yoon-Bo
YB
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D016015:Logistic Models; D008460:Meat / Q000032:analysis; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013754:Tetracyclines / Q000032:analysis*
D000900:Anti-Bacterial Agents; D013754:Tetracyclines