title: [Antisense oligonucleotides targeting protein kinase C alpha inhibits the proliferation of A549 cells].
abstract: OBJECTIVE
  To investigate the effects of antisense oligonucleotides (ASODN) targeting protein kinase C alpha (PKCalpha) on the proliferation of A549 cells.
  
  
  METHODS
  PKCalpha ASODN and random oligonucleotides (RODN) were transfected into A549 cells mediated by polyethyleneimine, and the proliferation and clone formation of A549 cells were detected by CCK-8 and clone formation assay, respectively. The expression of PKCalpha in the transfected cells was analyzed by RT-PCR and Western blotting.
  
  
  RESULTS
  Compared with those in the control group, PEI group and PEI-RODN group, the proliferation and clone formation of A549 cells treated with ASODN targeting PKCalpha were significantly inhibited (P<0.05). The expressions of PKCalpha mRNA and protein in PKCalpha ASODN-transfected A549 cells were significantly lower than those in the other 3 groups (P<0.05).
  
  
  CONCLUSIONS
  The PKCalpha ASODN mediated by PEI down-regutates the expression of PKCalpha gene and suppress the proliferation and clone formation of A549 cells.
authors.affiliation: Department of Biochemistry, Department of Respiratory Medicine, Guangzhou, China. yanyuxia68@126.com
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Yan
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Jiang
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Huang
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Wu
authors.forename: Chun-Lan
authors.initials: CL
authors.lastname: Lin
authors.forename: Feng-Yun
authors.initials: FY
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D051571:Protein Kinase C-alpha / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D051571:Protein Kinase C-alpha