Inadvertent recombinant human follicle stimulating hormone bolus instead of human chorionic gonadotrophin leads to the retrieval of competent oocytes in IVF program.
OBJECTIVE
To present a case where recombinant human FSH (r-hFSH) was accidentally used instead of hCG and resulted in oocyte maturation.


METHODS
Case report.


METHODS
A tertiary infertility center.


METHODS
A 36-year-old woman underwent IVF for infertility problems. The couple was unable to obtain a pregnancy during the previous 2 years.


METHODS
Oocyte retrieval after a r-hFSH bolus (two vials of Gonal-F 1050 IU) instead of the routinely used hCG.


METHODS
Oocyte maturity evaluation, fertilization after intracytoplasmic sperm injection, cleavage stage, and embryo quality.


RESULTS
A total of nine oocytes were retrieved; eight were metaphase II. Three eggs were fertilized by intracytoplasmic sperm injection, and four of the remaining MII oocytes were cryopreserved. All three oocytes fertilized and developed to three good-quality embryos that were transferred on day 2. No pregnancy was obtained from this fresh transfer cycle or from transferred embryos resulting from the thawing cycle.


CONCLUSIONS
A massive r-hFSH administration can lead to the retrieval of mature MII oocytes that are able to fertilize and give good-quality embryos to transfer.
Bianchi
Veronica
V
Tecnobios Procreazione, Centre for Reproductive Health, Casa di Cura Città di Udine, Udine, Italy.
Dal Prato
Luca
L
Maccolini
Andrea
A
Mazzone
Silvia
S
Borini
Andrea
A
D000328:Adult; D006063:Chorionic Gonadotropin / Q000627:therapeutic use; D004333:Drug Administration Routes; D004334:Drug Administration Schedule; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D007246:Infertility / Q000188:drug therapy*; D008508:Medication Errors; D054315:Oocyte Retrieval; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000627:therapeutic use
D006063:Chorionic Gonadotropin; D011994:Recombinant Proteins; D005640:Follicle Stimulating Hormone