Live birth from vitrified-warmed human oocytes fertilized with frozen-thawed testicular spermatozoa.
A couple with male infertility due to non-obstructive azoospermia were referred to the fertility centre for treatment. Testicular biopsy was performed on the male partner and testicular samples were frozen. The female partner underwent ovarian stimulation and 31 mature oocytes were recovered by ultrasound-guided vaginal aspiration. Twelve oocytes were cryopreserved by the Cryotop vitrification method and 19 oocytes were inseminated by intracytoplasmic sperm injection (ICSI) using frozen-thawed testicular spermatozoa. Nine out of 19 oocytes were fertilized and the resulting embryos were cryopreserved by slow freezing. Four months later, two out of six thawed embryos were transferred, but no pregnancy resulted. One year later, the couple decided to attempt pregnancy using vitrified oocytes and frozen testicular spermatozoa. Six vitrified-warmed oocytes were injected with frozen-thawed testicular spermatozoa and four were fertilized. On the day of transfer, two cleavage stage embryos (4-cell, 2-cell) were obtained. Serum beta-HCG test 14 days after embryo transfer was positive. Hormonal support for the established pregnancy was maintained with oestradiol and progesterone orally until 12 weeks of gestation. A healthy baby boy weighing 3.09 kg was delivered by elective Caesarean section at 38 weeks of gestation. This case report demonstrates that oocyte cryopreservation by the Cryotop vitrification method does not compromise oocyte developmental competence.
Liow
Swee Lian
SL
Embryonics International, Gleneagles Hospital, Annex Block #01-38, 6A Napier Road, Singapore.
Foong
Lian Cheun
LC
Chen
Nai Qing
NQ
Yip
Wan Yue
WY
Khaw
Chew Ling
CL
Kumar
Jothi
J
Vajta
Gábor
G
Ng
Soon Chye
SC
D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D005615:Freezing; D006358:Hot Temperature; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000166:cytology*