title: Up-regulation of oligodendrocyte precursor cell alphaV integrin and its extracellular ligands during central nervous system remyelination.
abstract: To determine the role of extracellular matrix molecules and their integrin ligands in CNS remyelination, we have examined in experimentally induced focal demyelinated lesions the expression of the two classes of integrins implicated in oligodendrocyte development and myelination: alpha6 laminin-binding integrins and alphaV integrins that bind a range of extracellular matrix proteins containing the -Arg-Gly-Asp- (RGD) recognition sequence. Only alphaV integrins were up-regulated during remyelination, being expressed on oligodendrocyte precursor cells during their recruitment into the lesion. Next, therefore, we examined the expression of extracellular matrix ligands for alphaV integrins and documented increased expression of tenascin-C, tenascin-R, fibronectin, and vitronectin. Taken together with our previous discovery of high levels of expression of another alphaV ligand, osteopontin, during remyelination in these lesions, our findings suggest that alphaV integrins make an important contribution to successful repair in the CNS.
authors.affiliation: MRC Centre for Stem Cell Biology and Regenerative Medicine and Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom. cz213@cam.ac.uk
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.forename: Stephen P J
authors.initials: SP
authors.lastname: Fancy
authors.forename: Robin J M
authors.initials: RJ
authors.lastname: Franklin
authors.forename: Charles
authors.initials: C
authors.lastname: ffrench-Constant
mesh_terms: D000818:Animals; D003711:Demyelinating Diseases / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D039564:Integrin alphaV / Q000378:metabolism*; D008024:Ligands; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000473:pathology; D009416:Nerve Regeneration / Q000502:physiology*; D009836:Oligodendroglia / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D013234:Stem Cells / Q000378:metabolism*; D019063:Tenascin / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology; D019096:Vitronectin / Q000378:metabolism
chemical_list: D005353:Fibronectins; D039564:Integrin alphaV; D008024:Ligands; D019063:Tenascin; D019096:Vitronectin; C074638:tenascin R