Recombinant protein production by a Kaiko-baculovirus system.
Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the application of a highly efficient and reproducible baculovirus expression system with high throughput using Kaiko (silkworm).
Nagaya
Hidekazu
H
Katakura Industries Co., Ltd., Saitama, Japan.
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D057166:High-Throughput Screening Assays / Q000379:methods; D008967:Molecular Biology / Q000379:methods; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D014162:Transfection
D011994:Recombinant Proteins