title: A vertebrate Polycomb response element governs segmentation of the posterior hindbrain.
abstract: Chromatin remodeling by Polycomb group (PcG) and trithorax group (trxG) proteins regulates gene expression in all metazoans. Two major complexes, Polycomb repressive complexes 1 and 2 (PRC1 and PRC2), are thought to mediate PcG-dependent repression in flies and mammals. In Drosophila, PcG/trxG protein complexes are recruited by PcG/trxG response elements (PREs). However, it has been unclear how PcG/trxG are recruited in vertebrates. Here we have identified a vertebrate PRE, PRE-kr, that regulates expression of the mouse MafB/Kreisler gene. PRE-kr recruits PcG proteins in flies and mouse F9 cells and represses gene expression in a PcG/trxG-dependent manner. PRC1 and 2 bind to a minimal PRE-kr region, which can recruit stable PRC1 binding but only weak PRC2 binding when introduced ectopically, suggesting that PRC1 and 2 have different binding requirements. Thus, we provide evidence that similar to invertebrates, PREs act as entry sites for PcG/trxG chromatin remodeling in vertebrates.
authors.affiliation: Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, ON, M5G 1X5, Canada.
authors.forename: Angela
authors.initials: A
authors.lastname: Sing
authors.forename: Dylan
authors.initials: D
authors.lastname: Pannell
authors.forename: Angelo
authors.initials: A
authors.lastname: Karaiskakis
authors.forename: Kendra
authors.initials: K
authors.lastname: Sturgeon
authors.forename: Malek
authors.initials: M
authors.lastname: Djabali
authors.forename: James
authors.initials: J
authors.lastname: Ellis
authors.forename: Howard D
authors.initials: HD
authors.lastname: Lipshitz
authors.forename: Sabine P
authors.initials: SP
authors.lastname: Cordes
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D002645:Chickens; D042002:Chromatin Assembly and Disassembly; D007446:Chromosome Inversion; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D051258:MafB Transcription Factor / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D063150:Polycomb Repressive Complex 1; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements; D012249:Rhombencephalon / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D051258:MafB Transcription Factor; C490406:Mafb protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C490239:Nnat protein, mouse; C066770:Pc protein, Drosophila; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1