Focus on the splicing of secretin GPCRs transmembrane-domain 7.
The family of G-protein coupled receptors (GPCRs) is one of the largest protein families in the mammalian genome with a fundamental role in cell biology. GPCR activity is finely tuned by various transcriptional, post-transcriptional and post-translational mechanisms. Alternative pre-mRNA splicing is now emerging as a crucial process regulating GPCR biological function. Intriguingly, this mechanism appears to extensively target the Secretin family of GPCRs, especially the exon that encodes a 14 amino acid sequence that forms the distal part of 7th transmembrane helix, and exhibits an unusually high level of sequence conservation among most Secretin GPCRs. Do the "TMD7-short" receptor variants have a role as novel regulators of GPCR signallng and, if so, what are the implications for hormonal actions and physiology?
Markovic
Danijela
D
GPCR Pathophysiology Group, Division of Endocrinology and Metabolism, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
Grammatopoulos
Dimitris K
DK
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D005091:Exons; D006801:Humans; D008958:Models, Molecular; D010802:Phylogeny; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D022821:RNA Splice Sites; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011964:Receptors, Gastrointestinal Hormone / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D012633:Secretin / Q000502:physiology; D016415:Sequence Alignment; D015398:Signal Transduction
D020033:Protein Isoforms; D022821:RNA Splice Sites; D043562:Receptors, G-Protein-Coupled; D011964:Receptors, Gastrointestinal Hormone; C029937:secretin receptor; D012633:Secretin