Immobilization of anti-galectin-3 onto polysiloxane-polyvinyl alcohol disks for tumor prostatic diseases diagnosis.
This work aimed to immobilize the antibody anti-galectin-3 onto polysiloxane-polyvinyl alcohol (POS-PVA) support, to evaluate its capacity to capture the serum antigen galectin-3 and to quantify by ELISA the antigen levels in sera from patients with prostatic adenocarcinoma (PA) and benign prostatic hyperplasia (BPH) and healthy individuals. Also, for comparative effect, the galectin-3 expression in the prostate tissue through immunohistochemistry was evaluated. The optical density (galectin-3 level) values established for the sera from PA and BPH patients were lower compared with those found for the healthy individuals. Galectin-3 immunohistochemically showed a significant increase and reduction of the cytoplasmatic protein expression in BPH and PA, respectively, compared with the normal prostate. These results showed that POS-PVA disks could be used as solid phase to immobilize serum galectins and in immunoassays procedures for the correspondent IgG anti-galectins detection in human sera.
de Melo-Júnior
Mario Ribeiro
MR
Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, Brazil. mariormj@gmail.com
Araújo-Filho
Jorge Luiz Silva
JL
Lins
Consuelo Antunes Barreto
CA
de Pontes-Filho
Nicodemos Teles
NT
de Carvalho
Luiz Bezerra
LB
D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D037502:Galectin 3 / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011467:Prostate / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000097:blood / Q000175:diagnosis; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis; D012833:Siloxanes / Q000737:chemistry*
D037502:Galectin 3; D012833:Siloxanes; D011142:Polyvinyl Alcohol