title: Prevotella dentasini sp. nov., a black-pigmented species isolated from the oral cavity of donkeys.
abstract: Four strains (NUM 1903(T), NUM 1904, NUM 1912 and NUM 1925) that were obligately anaerobic, pigmented, Gram-negative-staining rods were isolated from the oral cavity of donkeys. These strains were analysed using the Rapid ID 32A, API 20A and API ZYM systems, by DNA-DNA hybridization with other related species and by 16S rRNA gene sequencing. 16S rRNA gene sequence analysis showed that each of the new isolates was a member of the genus Prevotella and related to Prevotella multiformis PPPA21(T), showing about 93 % sequence similarity. Based on phylogenetic and phenotypic evidence, it is proposed that the four strains are representatives of a novel species, for which the name Prevotella dentasini sp. nov. is proposed. The type strain is NUM 1903(T) (=JCM 15908(T)=DSM 22229(T)).
authors.affiliation: Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Takada
authors.affiliation: Department of Oral Medicine, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: Department of Biochemistry, Tokyo Dental College, Mihama, Chiba 261-8502, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
authors.forename: Masatomo
authors.initials: M
authors.lastname: Hirasawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D018397:Equidae / Q000382:microbiology*; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010802:Phylogeny; D018720:Prevotella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology