[Effects of peritoneal dialysis solution on apoptosis and intracellular free calcium, cell surface ICAM-1 expression of rat peritoneal mesothelial cells].
OBJECTIVE
To investigate the effects of peritoneal dialysis solution (PDS) on apoptosis and intracellular free calcium([Ca(2+)]i), cell surface ICAM-1 expression of rat peritoneal mesothelial cells (RPMCs).


METHODS
The RPMCs apoptosis rate were determined by flow cytometry. [Ca(2+)]i in the cells were monitered the fluorescence at 528 nm by confocus laser microscopy. Cell surface ICAM-1 expression were detected by flow cytometry.


RESULTS
After PDS treatment for 1 h, the RPMCs apoptosis rate were increased. Such increase was more manifest with higher glucose concentration in PDS and longer treatment time of the cells. At the same times, after 3 hours, ICAM-1 expressions of the PDS containing glucose and mannitol are all increased. With the increase of glucose concentrations, the descend of [Ca(2+)]i levels were aggravated.


CONCLUSIONS
PDS containing high- concentration glucose can induce significant apoptosis of RPMCs in vitro. This may be related with the enhanced level of ICAM-1 expressions and the decreased level of [Ca(2+)]i. Which may due to the occurrence of peritoneal fibrosis and ultrafiltrate failure in patients suffering long term peritoneal dialysis.
Ren
Hao
H
Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Chen
Sheng-xiao
SX
Liu
Hong-fa
HF
Liu
Zheng-rong
ZR
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D015314:Dialysis Solutions / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D042541:Intracellular Space / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D010530:Peritoneal Dialysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D015314:Dialysis Solutions; D018799:Intercellular Adhesion Molecule-1; D005947:Glucose; D002118:Calcium