Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ.
OBJECTIVE
This in situ/ex vivo study aimed to analyse the impact of possible MMP-inhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion.


METHODS
Twelve volunteers took part in this cross-over and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoride-free toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60s with: 250 ppm F solution (SnF(2)/AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24, 100% Camellia Sinensis leaf extract, catechin concentration: 30+/-3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (microm). The data were analysed by two-way repeated measures ANOVA and Bonferroni post hoc test.


RESULTS
There was a significant difference between the conditions (EroxEro+Abr, p<0.001) and among the solutions (p<0.001). All solutions (F: 1.42+/-0.34; 1.73+/-0.50, chlorhexidine: 1.15+/-0.26; 1.59+/-0.32, green tea: 1.06+/-0.30; 1.54+/-0.55) significantly reduced the dentin wear when compared to control (2.00+/-0.55; 2.41+/-0.83) for both conditions. There were not significant differences among green tea extract, chlorhexidine and F solutions.


CONCLUSIONS
Thus, the possible MMP-inhibitors tested in this study seem to be a promising preventive measure to reduce dentin erosion-abrasion, but their mechanism of action needs to be investigated in further studies.
Magalhães
Ana Carolina
AC
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Wiegand
Annette
A
Rios
Daniela
D
Hannas
Angélica
A
Attin
Thomas
T
Buzalaf
Marília Afonso Rabelo
MA
D000328:Adult; D000588:Amines / Q000627:therapeutic use; D000818:Animals; D028241:Camellia sinensis; D002253:Carbonated Beverages / Q000009:adverse effects; D002392:Catechin / Q000627:therapeutic use; D002417:Cattle; D002710:Chlorhexidine / Q000627:therapeutic use*; D018592:Cross-Over Studies; D003804:Dentin / Q000187:drug effects; D004311:Double-Blind Method; D004338:Drug Combinations; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008297:Male; D061965:Matrix Metalloproteinase Inhibitors; D009067:Mouthwashes / Q000627:therapeutic use; D010936:Plant Extracts / Q000627:therapeutic use*; D011446:Prospective Studies; D014002:Tin Fluorides / Q000627:therapeutic use; D014072:Tooth Abrasion / Q000517:prevention & control*; D014077:Tooth Erosion / Q000517:prevention & control*; D014099:Toothbrushing / Q000009:adverse effects; D055815:Young Adult
D000588:Amines; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D009067:Mouthwashes; D010936:Plant Extracts; D014002:Tin Fluorides; C059813:Meridol; D002392:Catechin; D002710:Chlorhexidine