Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela.
The performances of 2 commercial enzyme-linked immunosorbent assay (ELISA) kits (PLATELIA Dengue NS1 AG and Dengue Early ELISA) and a rapid immunochromatography test (Dengue NS1 AG Strip) for detection of dengue NS1 protein were compared using a panel of 87 sera from viremic dengue patients, as well as 36 sera from patients with other acute febrile illnesses. PLATELIA was more sensitive and slightly less specific than Dengue Early ELISA (sensitivity, 71.3% versus 60.9%; specificity, 86.1% versus 94.3%, respectively). The strip test showed an overall sensitivity of 67.8% with a specificity of 94.4%. A lower sensitivity was observed with Dengue Early ELISA for dengue virus (DENV) type 4 (30%) and by the 3 tests for DENV type 2 (56.5%). The use of these kits allows for rapid and specific early diagnosis of dengue infection; however, their sensitivity for each serotype must be further evaluated to guarantee an accurate diagnosis, particularly in those regions where the 4 dengue serotypes are cocirculating.
Ramirez
Alvaro H
AH
Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Laboratorio de Biología de Virus, Caracas 1020A, Venezuela. alramire@ivic.ve
Moros
Zoila
Z
Comach
Guillermo
G
Zambrano
Julio
J
Bravo
Lola
L
Pinto
Belqys
B
Vielma
Silvana
S
Cardier
José
J
Liprandi
Ferdinando
F
D000208:Acute Disease; D000330:Aedes / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D016009:Chi-Square Distribution; D003715:Dengue / Q000097:blood / Q000276:immunology / Q000821:virology*; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000276:immunology; D014766:Viremia / Q000097:blood / Q000276:immunology / Q000821:virology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D017361:Viral Nonstructural Proteins