title: Rapid detection of Mycobacterium tuberculosis in sputum by Patho-TB kit in comparison with direct microscopy and culture.
abstract: The usefulness of a new rapid diagnostic test (Patho-TB) using antibodies specific to mycobacterial antigens was evaluated for the rapid discrimination between pulmonary tuberculosis (TB) and non-TB pulmonary diseases on sputa. One hundred sputa collected from 79 active TB patients and from 21 patients with non-TB pulmonary diseases (asthma and chronic obstructive pulmonary disease) were enrolled into the study and tested for the presence of Mycobacterium tuberculosis by Ziehl-Neelsen smear, Patho-TB kit, and Löwenstein-Jensen culture. The sensitivity, specificity, positive predictive value, and negative predictive value of the Patho-TB test were 95%, 100%, 100%, and 84%, respectively. Patho-TB test is simple, quick, and easy to perform. Its sensitivity, specificity, and positive predictive value are satisfactory. Therefore, it could be used as a screening test in poorly equipped laboratories of TB endemic areas.
authors.affiliation: Microbiology and Immunology Laboratory UR02SP13, CHU Farhat Hached, Sousse 4000, Tunisia.
authors.forename: Walid
authors.initials: W
authors.lastname: Ben-Selma
authors.forename: Imen
authors.initials: I
authors.lastname: Ben-Kahla
authors.forename: Manel
authors.initials: M
authors.lastname: Marzouk
authors.forename: Asma
authors.initials: A
authors.lastname: Ferjeni
authors.forename: Samira
authors.initials: S
authors.lastname: Ghezal
authors.forename: Moncef
authors.initials: M
authors.lastname: Ben-Said
authors.forename: Jalel
authors.initials: J
authors.lastname: Boukadida
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis; D001431:Bacteriological Techniques / Q000379:methods*; D016022:Case-Control Studies; D018929:Cell Culture Techniques / Q000379:methods; D003627:Data Interpretation, Statistical; D005260:Female; D006801:Humans; D008297:Male; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D011933:Reagent Kits, Diagnostic