title: Optimization of the structure of polyurethanes for bone tissue engineering applications.
abstract: Polyurethanes containing 22-70 wt.% hard segments were developed and evaluated for bone tissue engineering applications. Aliphatic poly(ester-urethanes) were synthesised from poly(epsilon-caprolactone) diol with different molecular masses (M= approximately 530, 1250 and 2000 Da), cycloaliphatic diisocyanate 4,4'-methylenebis(cyclohexyl isocyanate) and ethylene glycol as a chain extender. Changes in macromolecule order with increasing hard segment content were observed via modulated differential scanning calorimetry. Depending on the hard segment content, a gradual variation in polyurethane surface properties was revealed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and static contact angle measurements. As the hard segments content increased the polyurethane surface exhibited more phase separation, a higher content of urethane moieties and higher hydrophilicity. The biocompatibility results indicated that proliferation of human bone-derived cells (HBDC) cultured in vitro improved with increasing hard segment content while the osteogenic potential of HBDC decreased with increasing hard segment content.
authors.affiliation: Warsaw University of Technology, Faculty of Materials Science and Engineering, Warsaw, Poland. mbil@meil.pw.edu.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Bil
authors.forename: Joanna
authors.initials: J
authors.lastname: Ryszkowska
authors.forename: Piotr
authors.initials: P
authors.lastname: Woźniak
authors.forename: Krzysztof J
authors.initials: KJ
authors.lastname: Kurzydłowski
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Lewandowska-Szumieł
mesh_terms: D001842:Bone and Bones; D002152:Calorimetry, Differential Scanning; D011140:Polyurethanes; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis / Q000379:methods; D023822:Tissue Engineering; D014965:X-Rays
chemical_list: D011140:Polyurethanes