title: Expression of water and ion transporters in tracheal aspirates from neonates with respiratory distress.
abstract: OBJECTIVE
  The aim of the study was to determine whether neonatal respiratory distress is related to changes in water and ion transporter expression in lung epithelium.
  
  
  METHODS
  The study included 32 neonates on mechanical ventilation: 6 patients with normal lung X-rays (control group), eight with respiratory distress syndrome (RDS), eight with transient tachypnea of the newborn (TTN), 10 with abnormal lung X-rays (mixed group). The protein abundance of water channel AQP5, epithelial sodium channel (ENaC; alpha-, beta- and gamma-ENaC) and Na(+), K(+)-ATPase alpha1 were examined in tracheal aspirates using semiquantitative immunoblotting.
  
  
  RESULTS
  beta-ENaC level was significantly lower in RDS group compared with infants with TTN and infants in the control group. AQP5 expression was significantly higher in TTN compared with the infants with RDS and all other infants with abnormal lung X-rays.
  
  
  CONCLUSIONS
  Neonatal respiratory distress is associated with changes in beta-ENaC and AQP5 expression. The lower beta-ENaC expression may be one of the factors that predispose to the development of RDS. The higher AQP5 expression may provide the possibility for reabsorption of postnatal lung liquid, which contributes to quick recovery of infants with TTN.
authors.affiliation: Department of Woman & Child Health, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Marcoux
authors.forename: Martine
authors.initials: M
authors.lastname: Gineste
authors.forename: Mireille
authors.initials: M
authors.lastname: Vanpee
authors.forename: Marina
authors.initials: M
authors.lastname: Zelenina
authors.forename: Charlotte
authors.initials: C
authors.lastname: Casper
mesh_terms: D000818:Animals; D000906:Antibodies; D051402:Aquaporin 5 / Q000378:metabolism*; D016022:Case-Control Studies; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007231:Infant, Newborn; D007234:Infant, Premature; D017136:Ion Transport; D008168:Lung / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012120:Respiration Disorders / Q000378:metabolism; D012127:Respiratory Distress Syndrome, Newborn / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014132:Trachea / Q000166:cytology / Q000378:metabolism
chemical_list: D000906:Antibodies; D051402:Aquaporin 5; D053503:Epithelial Sodium Channels; D000254:Sodium-Potassium-Exchanging ATPase