Isolation and characterization of Malassezia spp. in healthy swine of different breeds.
Malassezia spp. genus is represented by several lipophilic yeasts, normally present on the skin of many warm-blooded vertebrates, including man. Swine are one of the less investigated animal species. The aim of the present work was to study the occurrence of Malassezia spp. in the external ear canal of 408 healthy swine of different breeds, under different breeding conditions. For this purpose N. 185 free-ranging wild boars, N. 107 large size pigs and 116 Cinta Senese breed were selected. Animals were of both genders, with age ranging from 8 months to 4 years. The subjects were culturally and molecularly checked for Malassezia spp. Ninety-two out of 408 animals scored positive for Malassezia yeasts (22.5%). Malassezia pachydermatis, Malassezia sympodialis and Malassezia furfur were recognized. M. pachydermatis was the sole species isolated from wild boars (12.9%), Cinta Senese (20.7%) and juvenile large size pigs (13.6%); 88% of large size breeds adult subjects scored positive for M. sympodialis (63.6%) and M. furfur (22.7%), respectively. The study focus on scarcely investigated epidemiological aspects of Malassezia spp. in this animal species.
Nardoni
S
S
Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, Viale delle Piagge, 2, 56100 Pisa, Italy. snardoni@vet.unipi.it
Merildi
V
V
Frangioni
S
S
Ariti
G
G
Verin
R
R
Vannucci
P
P
Mancianti
F
F
D000818:Animals; D001947:Breeding; D003881:Dermatomycoses / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004424:Ear Canal / Q000382:microbiology*; D005260:Female; D008290:Malassezia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology*