title: Global microsatellite content distinguishes humans, primates, animals, and plants.
abstract: Microsatellites are highly mutable, repetitive sequences commonly used as genetic markers, but they have never been studied en masse. Using a custom microarray to measure hybridization intensities of every possible repetitive nucleotide motif from 1-mers to 6-mers, we examined 25 genomes. Here, we show that global microsatellite content varies predictably by species, as measured by array hybridization signal intensities, correlating with established taxonomic relationships, and particular motifs are characteristic of one species versus another. For instance, hominid-specific microsatellite motifs were identified despite alignment of the human reference, Celera, and Venter genomic sequences indicating substantial variation (30-50%) among individuals. Differential microsatellite motifs were mainly associated with genes involved in developmental processes, whereas those found in intergenic regions exhibited no discernible pattern. This is the first description of a method for evaluating microsatellite content to classify individual genomes.
authors.affiliation: McDermott Center for Human Growth and Development of the University of Texas Southwestern Medical Center, Dallas, Texas, USA. Cristi.galindo@utsouthwestern.edu
authors.forename: C L
authors.initials: CL
authors.lastname: Galindo
authors.forename: L J
authors.initials: LJ
authors.lastname: McIver
authors.forename: J F
authors.initials: JF
authors.lastname: McCormick
authors.forename: M A
authors.initials: MA
authors.lastname: Skinner
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.forename: R A
authors.initials: RA
authors.lastname: Gelhausen
authors.forename: K
authors.initials: K
authors.lastname: Ng
authors.forename: N M
authors.initials: NM
authors.lastname: Kumar
authors.forename: H R
authors.initials: HR
authors.lastname: Garner
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D016678:Genome / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D002679:Pan troglodytes / Q000235:genetics; D010944:Plants / Q000235:genetics*; D011323:Primates / Q000235:genetics*; D013045:Species Specificity