title: Oxygen consumption, ventilation frequency and cytochrome c oxidase activity in blue cod (Parapercis colias) exposed to hydrogen sulphide or isoeugenol.
abstract: The effects of hydrogen sulphide (H(2)S) and isoeugenol exposure on activity, oxygen consumption (VO(2)), ventilation frequency (Vf) and cytochrome c oxidase activity in a teleost fish are reported. In H(2)S (200 microM Na(2)S) exposed animals VO(2) and Vf decreased significantly (both to 40% of resting) after 30 min, concurrent with a loss of equilibrium and narcosis. Post-flushing, VO(2) increased to resting values, but Vf remained depressed (P<0.05) until 30 min of recovery. Subsequently, equilibrium and mobility were regained accompanied by increases in VO(2) (66%) and Vf (15%) between 60-70 min of recovery. Isoeugenol (0.011 g L(-1)) exposed fish reached stage 4-5 of anaesthesia accompanied by decreases (P<0.05) in VO(2) (64%) and Vf (38%) by 35 min. Post-flushing, VO(2) and Vf recovered to resting values, followed by a rise (P<0.05) in VO(2) (45%) and Vf (25%). Overall, VO(2) in relation to the resting rate was reduced in isoeugenol treated animals. Conversely, VO(2) was increased (P<0.05) relative to the resting rate in H(2)S exposed fish. 20 and 200 microM Na(2)S reduced cytochrome c oxidase activity (P<0.05) in skeletal muscle and gill lamellae by between 69 and 97%, while isoeugenol had no effect in any tissue.
authors.affiliation: School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand. lgf16@student.canterbury.ac.nz
authors.forename: Leonard G
authors.initials: LG
authors.lastname: Forgan
authors.forename: Malcolm E
authors.initials: ME
authors.lastname: Forster
mesh_terms: D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D003243:Consciousness / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005054:Eugenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005880:Gills / Q000187:drug effects* / Q000201:enzymology; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology; D010101:Oxygen Consumption / Q000187:drug effects*; D010473:Perciformes / Q000378:metabolism*; D012123:Pulmonary Ventilation / Q000187:drug effects*; D015656:Respiratory Mechanics / Q000187:drug effects*; D053608:Stupor / Q000139:chemically induced; D013997:Time Factors
chemical_list: D000777:Anesthetics; D005054:Eugenol; C036643:isoeugenol; D003576:Electron Transport Complex IV; D006862:Hydrogen Sulfide