Suppressed-priming PCR, a novel concept of DNA quantification based on PCR kinetics.
The concept of an unbiased DNA quantification method is proposed based on PCR kinetics. A set of PCRs was performed with a dilution series of primers, which differently limited DNA amplification. The difference in amplification efficiency between the DNA templates reflected the estimates of their relative amounts. This concept might serve as a theoretical base for further development of accurate and robust PCR-based quantification methods.
Takemoto
Shuhei
S
National Institute for Agro-Environmental Sciences, 3-1-3 Kannon-dai, Tsukuba, Ibaraki 305-8604, Japan.
Okada
Hiroaki
H
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004247:DNA