Administration of anti-glucagon-like peptide-2 serum suppresses epithelial cell proliferation of the distal small intestine in weanling rats.
We investigated the effects of endogenous glucagon-like peptide-2 (GLP-2) on the development of intestinal mucosa in weanling rats. Three-week-old male weanling Sprague-Dawley rats were administered either anti-GLP-2 or normal rabbit serum every other day for 2 weeks. We then measured length, weight, and bromodeoxyuridine incorporation in the intestine on day 13 following the first injection. Administration of anti-GLP-2 serum significantly inhibited both epithelial proliferation in the distal ileum and elongation of the small intestine. These results suggest that intrinsic GLP-2 contributes to the growth of the small intestine during the weanling period.
Ishizuka
Satoshi
S
Hokkaido University, Sapporo, Japan. zuka@chem.agr.hokudai.ac.jp
Inafune
Ayako
A
Hira
Tohru
T
Izumi
Hirohisa
H
Ozawa
Kazuhiro
K
Takase
Mitsunori
M
Hara
Hiroshi
H
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D053767:Glucagon-Like Peptide 2 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D007082:Ileum / Q000166:cytology / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014886:Weaning
D053767:Glucagon-Like Peptide 2; D007106:Immune Sera