SIP30 is regulated by ERK in peripheral nerve injury-induced neuropathic pain.
ERK plays an important role in chronic neuropathic pain. However, the underlying mechanism is largely unknown. Here we show that in chronic constriction injury-treated rat spinal cords, up-regulation of SIP30 (SNAP25-interacting protein 30), which is involved in the development and maintenance of chronic constriction injury-induced neuropathic pain, correlates with ERK activation and that the up-regulation of SIP30 is suppressed by intrathecal delivery of the MEK inhibitor U0126. In PC12 cells, up-regulation of SIP30 by nerve growth factor is also dependent on ERK activation. We found that there is an ERK-responsive region in the rat sip30 promoter. Activation of ERK promotes the recruitment of the transcription factor cyclic AMP-response element-binding protein to the sip30 gene promoter. Taken together, our results provide a potential downstream target of ERK activation-mediated neuropathic pain.
Peng
Guangdun
G
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Han
Mei
M
Du
Yimin
Y
Lin
Anning
A
Yu
Lei
L
Zhang
Yuqiu
Y
Jing
Naihe
N
D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D000242:Cyclic AMP / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009437:Neuralgia / Q000209:etiology / Q000378:metabolism*; D016716:PC12 Cells; D059348:Peripheral Nerve Injuries; D051381:Rats; D020218:Response Elements; D013116:Spinal Cord; D014157:Transcription Factors; D015854:Up-Regulation
D002868:Chromosomal Proteins, Non-Histone; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C459407:Zwint protein, rat; D000242:Cyclic AMP; D048049:Extracellular Signal-Regulated MAP Kinases