title: Behavioral disinhibition and reduced anxiety-like behaviors in monoamine oxidase B-deficient mice.
abstract: Monoamine oxidase (MAO) B catalyzes the degradation of beta-phenylethylamine (PEA), a trace amine neurotransmitter implicated in mood regulation. Although several studies have shown an association between low MAO B activity in platelets and behavioral disinhibition in humans, the nature of this relation remains undefined. To investigate the impact of MAO B deficiency on the emotional responses elicited by environmental cues, we tested MAO B knockout (KO) mice in a set of behavioral assays capturing different aspects of anxiety-related manifestations, such as the elevated plus maze, defensive withdrawal, marble burying, and hole board. Furthermore, MAO B KO mice were evaluated for their exploratory patterns in response to unfamiliar objects and risk-taking behaviors. In comparison with their wild-type (WT) littermates, MAO B KO mice exhibited significantly lower anxiety-like responses and shorter latency to engage in risk-taking behaviors and exploration of unfamiliar objects. To determine the neurobiological bases of the behavioral differences between WT and MAO B KO mice, we measured the brain-regional levels of PEA in both genotypes. Although PEA levels were significantly higher in all brain regions of MAO B KO in comparison with WT mice, the most remarkable increments were observed in the striatum and prefrontal cortex, two key regions for the regulation of behavioral disinhibition. However, no significant differences in transcript levels of PEA's selective receptor, trace amine-associated receptor 1 (TAAR1), were detected in either region. Taken together, these results suggest that MAO B deficiency may lead to behavioral disinhibition and decreased anxiety-like responses partially through regional increases of PEA levels.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA.
authors.forename: Marco
authors.initials: M
authors.lastname: Bortolato
authors.forename: Sean C
authors.initials: SC
authors.lastname: Godar
authors.forename: Shieva
authors.initials: S
authors.lastname: Davarian
authors.forename: Kevin
authors.initials: K
authors.lastname: Chen
authors.forename: Jean C
authors.initials: JC
authors.lastname: Shih
mesh_terms: D000818:Animals; D001007:Anxiety / Q000235:genetics / Q000378:metabolism*; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D005106:Exploratory Behavior; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008995:Monoamine Oxidase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D010627:Phenethylamines / Q000378:metabolism; D017397:Prefrontal Cortex / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D012309:Risk-Taking; D000095383:Trace Amine-Associated Receptors
chemical_list: D008995:Monoamine Oxidase; D010627:Phenethylamines; D043562:Receptors, G-Protein-Coupled; D000095383:Trace Amine-Associated Receptors; C029261:phenethylamine