title: Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.
abstract: Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering, Bethesda, Maryland, USA.
authors.forename: Martin F
authors.initials: MF
authors.lastname: Hohmann-Marriott
authors.forename: Alioscka A
authors.initials: AA
authors.lastname: Sousa
authors.forename: Afrouz A
authors.initials: AA
authors.lastname: Azari
authors.forename: Svetlana
authors.initials: S
authors.lastname: Glushakova
authors.forename: Guofeng
authors.initials: G
authors.lastname: Zhang
authors.forename: Joshua
authors.initials: J
authors.lastname: Zimmerberg
authors.forename: Richard D
authors.initials: RD
authors.lastname: Leapman
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D055032:Electron Microscope Tomography / Q000379:methods*; D004912:Erythrocytes / Q000469:parasitology* / Q000648:ultrastructure*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010963:Plasmodium falciparum / Q000648:ultrastructure*