The effect of glycolic acid monomer ratio on the emulsifying activity of PLGA in preparation of protein-loaded SLN.
Our previous work demonstrated that lactic/glycolic acid copolymer (PLGA) was an efficient emulsifier for the primary w/o emulsion in the formulation of protein-loaded solid lipid nanoparticles (SLN) by w/o/w double emulsion-solvent evaporation technique. In this work, the effect of PLGA composition on the emulsifying activity was studied with PLGA of different lactic/glycolic acid ratios (90/10, 75/25, 50/50). The results demonstrated that the glycolic acid monomer ratio significantly affected the emulsifying activity of PLGA. Increasing the glycolic acid monomer ratio from 10% to 50% decreased the minimum PLGA content needed to produce stable w/o emulsions. With same PLGA contents, increase of the glycolic acid monomer ratio increased the stable time of the w/o emulsion, yielded smaller and narrower-distributed SLN, and enhanced the encapsulation efficiency and loading capacity.
Xie
Shuyu
S
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Road West, Beijing 100193, PR China.
Wang
Siliang
S
Zhu
Luyan
L
Wang
Fenghua
F
Zhou
WenZhong
W
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D004655:Emulsions; D006016:Glycolates / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009821:Oils / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D044366:Transition Temperature; D014867:Water / Q000737:chemistry
D004655:Emulsions; D006016:Glycolates; D008055:Lipids; D009821:Oils; D014867:Water; C031149:glycolic acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid