Effect of electromagnetic pulse exposure on brain micro vascular permeability in rats.
OBJECTIVE
To observe the effect of electromagnetic pulse (EMP) exposure on cerebral micro vascular permeability in rats.


METHODS
The whole-body of male Sprague-Dawley rats were exposed or sham exposed to 200 pulses or 400 pulses (1 Hz) of EMP at 200 kV/m. At 0.5, 1, 3, 6, and 12 h after EMP exposure, the permeability of cerebral micro vascular was detected by transmission electron microscopy and immunohistochemistry using lanthanum nitrate and endogenous albumin as vascular tracers, respectively.


RESULTS
The lanthanum nitrate tracer was limited to the micro vascular lumen with no lanthanum nitrate or albumin tracer extravasation in control rat brain. After EMP exposure, the lanthanum nitrate ions reached the tight junction, basal lamina and pericapillary tissue. Similarly, the albumin immunopositive staining was identified in pericapillary tissue. The changes in brain micro vascular permeability were transient, the leakage of micro vascular vessels appeared at 1 h, and reached its peak at 3 h, and nearly recovered at 12 h, after EMP exposure. In addition, the leakage of micro vascular was more obvious after exposure of EMP at 400 pulses than after exposure of EMP at 200 pulses.


CONCLUSIONS
Exposure to 200 and 400 pulses (1 Hz) of EMP at 200 kV/m can increase cerebral micro vascular permeability in rats, which is recoverable.
Ding
Gui-Rong
GR
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi, China.
Li
Kang-Chu
KC
Wang
Xiao-Wu
XW
Zhou
Yong-Chun
YC
Qiu
Lian-Bo
LB
Tan
Juan
J
Xu
Sheng-Long
SL
Guo
Guo-Zhen
GZ
D000818:Animals; D001921:Brain / Q000098:blood supply*; D002199:Capillary Permeability / Q000502:physiology*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004594:Electrophysiology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley