Contribution of mobile genetic elements to Desulfovibrio vulgaris genome plasticity.
The genome of Desulfovibrio vulgaris strain DePue, a sulfate-reducing Deltaproteobacterium isolated from heavy metal-impacted lake sediment, was completely sequenced and compared with the type strain D. vulgaris Hildenborough. The two genomes share a high degree of relatedness and synteny, but harbour distinct prophage and signatures of past phage encounters. In addition to a highly variable phage contribution, the genome of strain DePue contains a cluster of open-reading frames not found in strain Hildenborough coding for the production and export of a capsule exopolysaccharide, possibly of relevance to heavy metal resistance. Comparative whole-genome microarray analysis on four additional D. vulgaris strains established greater interstrain variation within regions associated with phage insertion and exopolysaccharide biosynthesis.
Walker
Christopher B
CB
Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
Stolyar
Sergey
S
Chivian
Dylan
D
Pinel
Nicolas
N
Gabster
Jeffrey A
JA
Dehal
Paramvir S
PS
He
Zhili
Z
Yang
Zamin Koo
ZK
Yen
Huei-Che B
HC
Zhou
Jizhong
J
Wall
Judy D
JD
Hazen
Terry C
TC
Arkin
Adam P
AP
Stahl
David A
DA
D001435:Bacteriophages / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D016969:Desulfovibrio vulgaris / Q000145:classification / Q000235:genetics*; D016680:Genome, Bacterial; D044404:Genomic Islands; D020071:Interspersed Repetitive Sequences; D046228:Microarray Analysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics
D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial