Quantitative profiling method for oxylipin metabolome by liquid chromatography electrospray ionization tandem mass spectrometry.
Cyclooxygenase, lipoxygenase, and epoxygenase derived oxylipins, especially eicosanoids, play important roles in many physiological processes. Assessment of oxidized fatty acid levels is important for understanding their homeostatic and pathophysiological roles. Most reported methods examine these pathways in isolation. The work described here employed a solid phase extraction-liquid chromatography-electrospray ionization MS/MS (SPE-LC-ESI MS/MS) method to monitor these metabolites. In 21 min, 39 oxylipins were quantified along with eight corresponding internal standards. The limits of quantification were between 0.07 and 32 pg (20 pM-10 nM). Finally, the validated method was used to evaluate oxylipin profiles in lipopolysaccharide-exposed mice, an established septic inflammatory model. The method described here offers a useful tool for the evaluation of complex regulatory oxylipin responses in in vitro or in vivo studies.
Yang
Jun
J
Department of Entomology and Cancer Center, University of California, One Shields Avenue, Davis, California 95616, USA.
Schmelzer
Kara
K
Georgi
Katrin
K
Hammock
Bruce D
BD
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008070:Lipopolysaccharides / Q000494:pharmacology; D055442:Metabolome / Q000187:drug effects*; D051379:Mice; D054883:Oxylipins / Q000032:analysis / Q000737:chemistry*; D018805:Sepsis / Q000378:metabolism; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D008070:Lipopolysaccharides; D054883:Oxylipins