[Effects of Hax-1 siRNA on sensitivity to chemotherapy of melanoma A375 cells].
OBJECTIVE
To investigate the effects of Hax-1 siRNA on sensitivity to chemotherapy of melanoma A375 cells.


METHODS
The effects of Hax-1 siRNA combined with cisplatin(DDP) or Matrine(Mat) on proliferation, apoptosis of A375 cells and active forms of caspase-3 were detected with MTT assay, Annexin V-FITC/PI staining with FCM, Hoechst 33258 staining, and Western blot respectively.


RESULTS
The inhibition of proliferation , induction of apoptosis and the expression of active caspase-3 forms in DDP or Mat treated A375 cells transfected with Hax-1 siRNA were all significantly higher than those in untransfected A375 cells or A375 cells transfected with control siRNA (P<0.05). The distinctive apoptotic morphology was observed in Mat or DDP treated A375 cells transfected with Hax-1 siRNA.


CONCLUSIONS
The data from our current study indicate that Hax-1 siRNA could enhance the effects of DDP or Mat on inhibition of melanoma A375 cell growth and induction apoptosis, which may be caused by the increased expression of active forms of caspase-3.
Li
Wen-bin
WB
Department of Dermatology, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710004, China.
Feng
Jie
J
Yan
Xiao-ning
XN
Zhang
Cai-qing
CQ
D048868:Adaptor Proteins, Signal Transducing; D000470:Alkaloids / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008545:Melanoma / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011807:Quinolizines / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D000093842:Matrines
D048868:Adaptor Proteins, Signal Transducing; D000470:Alkaloids; D000970:Antineoplastic Agents; C104759:HAX1 protein, human; D011506:Proteins; D011807:Quinolizines; D034741:RNA, Small Interfering; D053148:Caspase 3; D002945:Cisplatin; D000093842:Matrines