Rhinovirus-induced modulation of gene expression in bronchial epithelial cells from subjects with asthma.
Rhinovirus (RV) infections trigger asthma exacerbations. Genome-wide expression analysis of RV1A-infected primary bronchial epithelial cells from normal and asthmatic donors was performed to determine whether asthma is associated with a unique pattern of RV-induced gene expression. Virus replication rates were similar in cells from normal and asthmatic donors. Overall, RV downregulated 975 and upregulated 69 genes. Comparisons of transcriptional profiles generated from microarrays and confirmed by quantitative reverse transcription PCR and cluster analysis showed some up- and downregulated genes in asthma cells involved in immune responses (IL1B, IL1F9, IL24, and IFI44) and airway remodeling (LOXL2, MMP10, FN1). Notably, most of the asthma-related differences in RV-infected cells were also present in the cells before infection. These findings suggest that differences in RV-induced gene expression profiles of cells from normal and mild asthmatic subjects could affect the acute inflammatory response to RV, and subsequent airway repair and remodeling.
Bochkov
Y A
YA
Department of Pediatrics, University of Wisconsin, Madison, Wisconsin, USA. yabochkov@wisc.edu
Hanson
K M
KM
Keles
S
S
Brockman-Schneider
R A
RA
Jarjour
N N
NN
Gern
J E
JE
D000328:Adult; D056151:Airway Remodeling / Q000235:genetics; D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis / Q000235:genetics; D001249:Asthma / Q000150:complications / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D053506:Matrix Metalloproteinase 10 / Q000096:biosynthesis / Q000235:genetics; D046228:Microarray Analysis; D010850:Picornaviridae Infections / Q000150:complications / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D012229:Rhinovirus / Q000502:physiology*; D014779:Virus Replication
D016207:Cytokines; D000594:Amino Acid Oxidoreductases; C119091:LOXL2 protein, human; D053506:Matrix Metalloproteinase 10