title: [Application of bioinformatics analysis of signal peptide in the identification of Neurospora crassa phyA gene].
abstract: OBJECTIVE
  To identify the function of the gene encoding Neurospora crassa EAA33149.1 protein which has 46.85% similarity with Aspergillus niger phA gene.
  
  
  METHODS
  The bioinformatics analysis was conducted using the prediction algorithms SignalP v3.0, arginine and lysine propeptide cleavage sites in eukaryotic protein sequence prediction algorithms ProP 1.0 server, transmembrane domain prediction algorithms Tmpred and TMHMM v2.0, potential GP I-anchor sites prediction algorithm big-P I Predictor and the subcellular protein location prediction algorithms TargetP v1.01. According to the analysis results, the gene was cloned into Saccharomyces cerevisiae.
  
  
  RESULTS
  The signal peptide, the cleavage site and the secretion pathway were determined, and the expressed recombinant protein with 54,000 displayed phytase activity.
  
  
  CONCLUSIONS
  The gene has been identified to encode N. crassa phyA.
authors.affiliation: Department of Biology, Shantou University Medical Colllege, Shantou, China. sunpingnanstu@yahoo.com.cn
authors.forename: Ping-Nan
authors.initials: PN
authors.lastname: Sun
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zhou
authors.forename: Zheng-Xiang
authors.initials: ZX
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D019295:Computational Biology / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D051942:Phytochrome A / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D051942:Phytochrome A; D011994:Recombinant Proteins