Culturing ovarian somatic and germline stem cells of Drosophila.
This unit describes how to collect, culture, and establish stable cell lines of ovarian somatic and germline stem cells of Drosophila. We also describe a protocol for culturing embryonic cells that overexpress growth factors, which serve as a source for conditioned medium.
Niki
Yuzo
Y
Ibaraki University, Ibaraki, Japan.
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004330:Drosophila / Q000166:cytology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D010053:Ovary / Q000166:cytology*; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D014020:Tissue Extracts; D016707:Tissue Fixation
D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D014020:Tissue Extracts