Flow cytometry discrimination between bacteria and clay-humic acid particles during growth-linked biodegradation of phenanthrene by Pseudomonas aeruginosa 19SJ.
Abstract Methods that quickly assess microbial density and aggregation in soil and sediments are needed in environmental microbiology. We report a flow cytometry method that uses the green and orange emission of the fluorochrome SYTO-13 to discriminate between bacteria and clay-humic acid particles. This approach distinguishes single or clustered bacteria, and clusters of bacteria and abiotic particles during the growth of the biosurfactant-producing strain Pseudomonas aeruginosa 19SJ on solid phenanthrene in the presence of humic acid-clay complexes.
Resina-Pelfort
Olga
O
Departament de Microbiologia, Universitat de Barcelona, Avenida Diagonal 645, 08028 Barcelona, Spain.
García-Junco
Marta
M
Ortega-Calvo
José Julio
JJ
Comas-Riu
Jaume
J
Vives-Rego
Josep
J