title: Mucosal expression of ENaC and AQP in experimental otitis media induced by Eustachian tube obstruction.
abstract: OBJECTIVE
  We investigated the expression of the epithelial sodium channel (ENaC) and aquaporins (AQPs) in the middle ear mucosa of a rat model of otitis media with effusion caused by surgical obstruction of the Eustachian tube.
  
  
  METHODS
  Sixty-four rats were randomly assigned to either undergo unilateral Eustachian tube obstruction (groups 1, 2, and 3) or to undergo no procedure (control group). Bony Eustachian tubes were approached through ventral incisions and obstructed with electrocautery. On days 14, 28, and 56, the ears were evaluated, and the rats were sacrificed for otoscopic evaluation and real-time RT-PCR. Immunohistochemistry was done for ENaC-alpha and AQP-1.
  
  
  RESULTS
  The level of ENaC-alpha expression decreased 0.28- and 0.73-fold at 2 and 4 weeks, respectively, but increased 1.48-fold at 8 weeks (p<0.05). The change in ENaC-beta expression at 2 weeks was insignificant. However, the level of ENaC-beta expression increased 3.17- and 7.85-fold at 4 and 8 weeks, respectively (p<0.05). The level of ENaC-gamma expression increased 1.51-, 4.82- and 14.79-fold at 2, 4 and 8 weeks, respectively (p<0.05). The level of AQP-1 expression decreased 0.10- and 0.04-fold at 4 and 8 weeks, respectively (p<0.05). The change in AQP4 expression at 4 and 8 weeks was insignificant (p>0.05). The pattern of immunoreactivity of ENaC-alpha and AQP-1 was similar with that of gene expression.
  
  
  CONCLUSIONS
  The experimental methods provoked reproducible otitis media with effusion. This model is well suited for studies of middle ear homeostasis during disease pathogenesis. Middle ear mucosa homeostasis is altered significantly by ETO, and the subunits of AQP proteins show a characteristic expression pattern over time.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University International Hospital, Goyang, Gyeonggi, Republic of Korea.
authors.forename: Jae-Jun
authors.initials: JJ
authors.lastname: Song
authors.forename: Seong Keun
authors.initials: SK
authors.lastname: Kown
authors.forename: Eo-Jin
authors.initials: EJ
authors.lastname: Kim
authors.forename: Yung-Seop
authors.initials: YS
authors.lastname: Lee
authors.forename: Bo-Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Chae
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004432:Ear, Middle / Q000378:metabolism*; D053503:Epithelial Sodium Channels / Q000096:biosynthesis*; D005064:Eustachian Tube / Q000601:surgery; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism*; D010034:Otitis Media with Effusion / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020346:Aquaporins; D053503:Epithelial Sodium Channels