title: Preparation and epitope mapping of a monoclonal antibody against Tri a Bd 27K, a major wheat allergen.
abstract: We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence (154)VPWVVVDGKPL(164) of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Komiyama
authors.forename: Miki
authors.initials: M
authors.lastname: Hiemori
authors.forename: Masumi
authors.initials: M
authors.lastname: Kimoto
authors.forename: Makiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamashita
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tsuji
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D018604:Epitope Mapping; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D017931:DNA Primers; C539494:Tri a Bd 27K allergen, Triticum aestivum