Prevotella dentasini sp. nov., a black-pigmented species isolated from the oral cavity of donkeys.
Four strains (NUM 1903(T), NUM 1904, NUM 1912 and NUM 1925) that were obligately anaerobic, pigmented, Gram-negative-staining rods were isolated from the oral cavity of donkeys. These strains were analysed using the Rapid ID 32A, API 20A and API ZYM systems, by DNA-DNA hybridization with other related species and by 16S rRNA gene sequencing. 16S rRNA gene sequence analysis showed that each of the new isolates was a member of the genus Prevotella and related to Prevotella multiformis PPPA21(T), showing about 93 % sequence similarity. Based on phylogenetic and phenotypic evidence, it is proposed that the four strains are representatives of a novel species, for which the name Prevotella dentasini sp. nov. is proposed. The type strain is NUM 1903(T) (=JCM 15908(T)=DSM 22229(T)).
Takada
Kazuko
K
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
Hayashi
Kazuhiko
K
Department of Oral Medicine, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
Sato
Yutaka
Y
Department of Biochemistry, Tokyo Dental College, Mihama, Chiba 261-8502, Japan.
Hirasawa
Masatomo
M
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
D000818:Animals; D001483:Base Sequence; D018397:Equidae / Q000382:microbiology*; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010802:Phylogeny; D018720:Prevotella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology