title: [Insertion of TC motif into hepatitis B virus core protein c/e1 site does not affect the expression of S and e antigen].
abstract: OBJECTIVE
  To investigate whether insertion of TC motif into hepatitis B virus (HBV) core protein c/e1 site affects the expression of S and e antigen.
  
  
  METHODS
  Different oligonucleotides encoding TC motif were inserted into the c/e1 site of the core gene of a 1.3 copy wild-type HBV genome vector. HepG2 cells were divided into several groups of cells to transiently transfect with the wild-type and mutant HBV vectors, respectively. In each group, the expression level of core protein inside cells was detected by western blotting, and the levels of S and e antigen in culture medium were analyzed by ELISA assay.
  
  
  RESULTS
  Western blotting showed that these TC-tagged core proteins were expressed at similar level of wild-type one. ELISA assay indicated that the level of S and e antigen in culture medium of different groups were not significantly different.
  
  
  CONCLUSIONS
  Insertion of TC motif into HBV core protein c/e1 site does not interference with the expression of viral protein encoded by HBV genome.
authors.affiliation: Institute of Liver Diseases, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Lin
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Cheng
authors.forename: Yu-hu
authors.initials: YH
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Chang
authors.forename: Jin-jian
authors.initials: JJ
authors.lastname: Yao
authors.forename: Ju-sheng
authors.initials: JS
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D006512:Hepatitis B Core Antigens / Q000235:genetics* / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014758:Viral Core Proteins / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins