title: Trypsin-gold nanoparticle conjugates: binding, enzymatic activity, and stability.
abstract: Nanomaterials may interact with biomolecules in various ways and change their bioactivities. Here, we report on how gold nanoparticles (AuNPs) affect a most important protease, trypsin. After simple mixing of the trypsin and AuNPs solution the average diameter is 20 nm under enzyme friendly conditions (pH 8.0); the bond between trypsin and AuNPs was detected by UV-vis spectroscopy. The further protease assay of trypsin, before and after mixing with the AuNPs solution, pointed out an improved performance in terms of enzyme activity and stability.
authors.affiliation: School of Life Sciences, Sichuan University, Sichuan, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Lv
authors.forename: Engao
authors.initials: E
authors.lastname: Zhu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Su
authors.forename: Qingnuan
authors.initials: Q
authors.lastname: Li
authors.forename: Wenxin
authors.initials: W
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
mesh_terms: D001665:Binding Sites; D004789:Enzyme Activation; D004795:Enzyme Stability; D006046:Gold / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011485:Protein Binding; D014357:Trypsin / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D006046:Gold; D014357:Trypsin