title: High-level expression, purification and characterization of a recombinant medium-temperature alpha-amylase from Bacillus subtilis.
abstract: Alpha-amylases are important industrial enzymes with a wide range of applications. Although medium-temperature alpha amylase (AmyE) has some practical advantages, its low yield has limited its applications. When an amyE gene from Bacillus subtilis BF768 was cloned into vector pWB980 and over-expressed in B. subtilis WB600, high activities (723 U ml(-1)) of secreted AmyE were produced. Recombinant AmyE was purified to a specific activity of 36 U mg(-1) having optimal activity at pH 6.0 and 60 degrees C.
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin, 300457, People's Republic of China.
authors.forename: Yihan
authors.initials: Y
authors.lastname: Liu
authors.forename: Fuping
authors.initials: F
authors.lastname: Lu
authors.forename: Guanqun
authors.initials: G
authors.lastname: Chen
authors.forename: Crystal L
authors.initials: CL
authors.lastname: Snyder
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Jianling
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D013696:Temperature; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D000516:alpha-Amylases