title: A novel isoquinoline alkaloid, DD-carboxypeptidase inhibitor, with antibacterial activity isolated from Streptomyces sp. 8812. Part II: Physicochemical properties and structure elucidation.
abstract: A novel antimicrobial agent labeled JS-1, being a member of isoquinoline alkaloids, of molecular formula C10H9NO4 was isolated from the culture broth of Streptomyces sp. 8812. In this study, we present the structure based on physicochemical and spectroscopic NMR investigations and on quantum chemical structure modeling. The structure of a molecule suggests the biosynthetic path starting from 3'-hydroxy tyrosine. The synthesis was undertaken and it resulted in NMR data that fully agree with the presented analysis.
authors.affiliation: Independent Laboratory of Streptomycetes and Fungi Imperfecti, National Institute of Public Health-National Institute of Hygiene, Warsaw, Poland. jsolecka@pzh.gov.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Solecka
authors.forename: Jerzy
authors.initials: J
authors.lastname: Sitkowski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bocian
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Bednarek
authors.forename: Robert
authors.initials: R
authors.lastname: Kawecki
authors.forename: Lech
authors.initials: L
authors.lastname: Kozerski
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D002627:Chemistry, Physical; D003470:Culture Media; D005285:Fermentation; D007546:Isoquinolines / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D013302:Streptomyces / Q000145:classification* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D003470:Culture Media; D007546:Isoquinolines; D002268:Carboxypeptidases; C039109:isoquinoline