title: Comparison of monoclonal and polyclonal antibodies for the detection of canine IgG1 and IgG2, and associations with infection outcome in Leishmania infantum naturally infected dogs.
abstract: In murine models of leishmaniasis, IgG subclass expression is a proxy measure for Th1/Th2 cellular immune response bias. However, in dogs, the reservoir of zoonotic visceral leishmaniasis, no consistent association has been described between IgG subclass ratios and disease resistance. Inconsistent results may reflect lack of specificity of commonly used commercial antibodies. Our aim was to measure IgG1 and IgG2 responses to crude Leishmania antigen using commercial polyclonal antibodies for comparison with a panel of commercially unavailable monoclonal antibodies, in a cohort of 60 naturally infected dogs, and to compare associations between subclass responses and clinical or parasitological outcomes. IgG1 and IgG2, measured by both antibodies, were higher in clinically symptomatic than in asymptomatic dogs (P</=0.03), reflecting general upregulation of IgG in infected dogs. Unlike the murine model, canine IgG2:IgG1 ratios were not predictive of clinical or parasitological outcomes of infection. Associations between subclass levels and positivity by bone marrow culture and PCR were not consistent when measured with different antibodies. Further research is needed to re-evaluate the specificity of commercially available IgG subclass antibodies.
authors.affiliation: Populations and Disease Research Group, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK. c.carson@warwick.ac.uk
authors.forename: Connor
authors.initials: C
authors.lastname: Carson
authors.forename: Rupert J
authors.initials: RJ
authors.lastname: Quinnell
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Day
authors.forename: Orin
authors.initials: O
authors.lastname: Courtenay
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004197:Disease Reservoirs / Q000469:parasitology; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood*; D018314:Leishmania infantum; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000662:veterinary*; D011379:Prognosis; D015854:Up-Regulation
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G