title: The influence of antibodies on Staphylococcus epidermidis adherence to polyvinylpyrrolidone-coated silicone elastomer in experimental biomaterial-associated infection in mice.
abstract: Biomaterial-associated infection (BAI) is a major problem in modern medicine, and is often caused by Staphylococcus epidermidis. We aimed to raise monoclonal antibodies (mAbs) against major surface protein antigens of S. epidermidis, and to assess their possible protective activity in experimental BAI. Mice were vaccinated with a cell wall protein preparation of S. epidermidis. A highly immunodominant antigen was identified as Accumulation-associated protein (Aap). mAbs against Aap and against surface-exposed lipoteichoic acid (LTA) were used for passive immunization of mice in experimental biomaterial-associated infection. Neither anti-Aap nor anti-LTA mAbs showed protection. Either with or without antibodies, tissue surrounding the implants was more often culture positive than the implants themselves, but bacterial adherence to the implants was significantly increased in mice injected with anti-LTA. In vitro, anti-Aap and anti-LTA did show binding to S. epidermidis, but no opsonic activity was observed. We conclude that antibodies against S. epidermidis LTA or Aap showed no opsonic activity and did not protect mice against BAI. Moreover, the increase in binding to implanted biomaterial suggests that passive immunization may increase the risk for BAI.
authors.affiliation: Department of Medical Microbiology, Center of Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Corine A N
authors.initials: CA
authors.lastname: Broekhuizen
authors.forename: Leonie
authors.initials: L
authors.lastname: de Boer
authors.forename: Kim
authors.initials: K
authors.lastname: Schipper
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Jones
authors.forename: Shan
authors.initials: S
authors.lastname: Quadir
authors.forename: Robert G
authors.initials: RG
authors.lastname: Feldman
authors.forename: Christina M J E
authors.initials: CM
authors.lastname: Vandenbroucke-Grauls
authors.forename: Sebastian A J
authors.initials: SA
authors.lastname: Zaat
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000009:adverse effects* / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D011205:Povidone / Q000009:adverse effects* / Q000737:chemistry; D012826:Silicone Elastomers / Q000009:adverse effects* / Q000737:chemistry; D013203:Staphylococcal Infections / Q000209:etiology* / Q000276:immunology* / Q000517:prevention & control; D013212:Staphylococcus epidermidis / Q000187:drug effects*
chemical_list: D000911:Antibodies, Monoclonal; D020099:Coated Materials, Biocompatible; D012826:Silicone Elastomers; D011205:Povidone