title: [Killer immunoglobulin-like receptor gene genotype and expression pattern in NK-92MI and K562 cells].
abstract: OBJECTIVE
  To make clear the repertoire of Killer immunoglobulin-like receptor at the level of DNA and RNA in NK-92MI and K562 cells and compare KIRs gene genotype and expression patterns in both cells.
  
  
  METHODS
  KIRs gene genotype, include KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 3DL1, 3DL2, 3DL3, 3DS1, 2DP1, 3DP1, was analyzed by PCR. KIRs gene phenotype, include KIR2DL1, KIR2DL2, KIR2DL3, KIR3DL1, KIR2DS1, KIR2DS3, KIR2DS2/4 gene, was determined by RT-PCR and sequencing.
  
  
  RESULTS
  KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5A, 2DS2, 2DS4*003-006, 2DS5, 3DL1, 3DL2 and 3DL3 gene were present in K562 cells, but all corresponding receptors were not expressed by K562 cells. KIR2DL1, 2DL2, 2DL3, 2DL4, 2DS2, 2DS4*001/002, 2DS4*003-006, 3DL1, 3DL2 and 3DL3 gene were present in NK-92MI cells, but only transcripts of KIR2DL1, KIR2DL3 and KIR2DS2/4 were detectable.
  
  
  CONCLUSIONS
  KIR2DL1, 2DL2, 2DL3, 2DL4, 2DS2, 2DS4*003-006, 3DL1, 3DL2 and 3DL3 gene were present in both K562 and NK-92MI cells. All corresponding KIR receptors were not expressed by K562 cells and only partial KIRs by NK-92MI cells. The repertoire of KIRs were specific for K562 and NK-92MI cells.
authors.affiliation: Department of Hematology, PLA General Hospital of China, Beijing 100853, China. lipingdou@yahoo.com
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Dou
authors.forename: Wan-ming
authors.initials: WM
authors.lastname: DA
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Jing-fen
authors.initials: JF
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Quan-shun
authors.initials: QS
authors.lastname: Wang
authors.forename: Chun-ji
authors.initials: CJ
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural; D054340:Receptors, KIR / Q000235:genetics* / Q000276:immunology
chemical_list: D054340:Receptors, KIR