Structual analysis of neutral and acidic xylooligosaccharides from hardwood kraft pulp, and their utilization by intestinal bacteria in vitro.
We established a method for producing two types of xylooligosaccharides by treating kraft pulp derived from hardwood such as eucalyptus with xylanase, analyzed the structure by MALDI-TOF-MS/MS, and conducted an in vitro study on utilization by intestinal bacteria. One type was a neutral xylooligosaccharide (XOS), a xylooligomer in the D.P. range of dimer to approximately 17-mer, containing approximately 12% xylobiose. The other was an acidic xylooligosaccharide (U-XOS), a xylooligomer in the D.P. range of dimer to approximately 17-mer, with a major side chain of 4-O-methyl-glucuronic acid and other side chains of glucuronic acid and 2-O-galactopyranosyl-4-O-methyl-glucurono acid. The in vitro utilization study with intestinal bacteria showed that XOS was specifically utilized by Bifidobacterium, whereas U-XOS was utilized by only a few types of bacteria. In a fecal batch culture, both XOS and U-XOS selectively increased the population of Bifidobacterium. The present study proposes a new method for industrial production of XOS and U-XOS, and indicates the utility and functionality.
Ohbuchi
Takayuki
T
R & D Laboratories Headquarters, Oji Paper Co., Ltd., 1-10-6 Shinonome, Koto-ku, Tokyo 135-8558, Japan. takayuki-ohbuchi@ojipaper.co.jp
Takahashi
Tetsunari
T
Azumi
Naoya
N
Sakaino
Makoto
M
D001644:Bifidobacterium / Q000378:metabolism*; D002236:Carbohydrate Conformation; D007422:Intestines / Q000382:microbiology*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D013783:Textile Industry; D014934:Wood
D009844:Oligosaccharides