title: Incidence of chromosomal mosaicism in morphologically normal nonhuman primate preimplantation embryos.
abstract: OBJECTIVE
  To establish the exact rates of chromosomal mosaicism in morphologically normal rhesus macaque embryos by determining the chromosomal complement of all blastomeres.
  
  
  METHODS
  Retrospective rhesus monkey IVF study.
  
  
  METHODS
  Academic laboratory and primate research center.
  
  
  METHODS
  Young fertile rhesus macaque females.
  
  
  METHODS
  Morphologically normal in vitro-produced rhesus macaque embryos were dissociated and cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y.
  
  
  METHODS
  The incidence and extent of chromosomal mosaicism in rhesus macaque preimplantation embryos.
  
  
  RESULTS
  Seventy-seven preimplantation embryos, displaying normal morphology and development, from 17 young rhesus macaque females were analyzed. Overall, 39 embryos (50.6%) were normal, 14 embryos (18.2%) were completely abnormal, and 24 embryos (31.2%) were mosaic. Of the 226 blastomeres analyzed in the mosaic group, 110 blastomeres (48.7%) were normal.
  
  
  CONCLUSIONS
  The observed rate of mosaicism in good-quality rhesus embryos resembles previously documented frequencies in poor-quality human preimplantation embryos. A high incidence of mosaicism may limit the diagnostic accuracy of preimplantation genetic diagnosis.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: Cathérine
authors.initials: C
authors.lastname: Dupont
authors.forename: James
authors.initials: J
authors.lastname: Segars
authors.forename: Alan
authors.initials: A
authors.lastname: DeCherney
authors.forename: Barry D
authors.initials: BD
authors.lastname: Bavister
authors.forename: D Randall
authors.initials: DR
authors.lastname: Armant
authors.forename: Carol A
authors.initials: CA
authors.lastname: Brenner
mesh_terms: D000782:Aneuploidy; D000818:Animals; D001755:Blastocyst; D001757:Blastomeres / Q000648:ultrastructure; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D008253:Macaca mulatta / Q000196:embryology / Q000235:genetics*; D009030:Mosaicism; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000592:standards; D012189:Retrospective Studies