title: synephrine, a component of Evodiae Fructus, constricts isolated rat aorta via adrenergic and serotonergic receptors.
abstract: We investigated the effects of Evodiae Fructus and synephrine, one of the components of Evodiae Fructus, on blood vessels. We found that Evodiae Fructus (1 x 10(-6) - 3 x 10(-4) g/mL) had constrictive effects on rat aorta. The vasoconstrictive effects of Evodiae Fructus were significantly inhibited by pretreatment with prazosin (adrenergic alpha(1)-receptor antagonist), BRL15572 [5-hydroxytryptamine (5-HT)(1D) antagonist], and ketanserin (5-HT(2A) antagonist), but its vasoconstrictive effects were not inhibited by pretreatment with SB216641 (5-HT(1B) antagonist) or propranolol (adrenergic beta-receptor antagonist). These results suggest that Evodiae Fructus constricts rat aorta via adrenergic and serotonergic receptors. We also investigated the constrictive effects of synephrine on blood vessels. The vasoconstrictive effects of synephrine (1 x 10(-7) - 3 x 10(-5) mol/L) were significantly inhibited by pretreatment with prazosin, BRL15572, and ketanserin. However, its constrictive effects were not inhibited by pretreatment with SB216641 and propranolol. The pA(2) values of prazosin or ketanserin were nearly equal between Evodiae Fructus and synephrine. Because the constrictive effects of both Evodiae Fructus and synephrine were exerted via adrenergic alpha(1)-receptors and serotonergic (5-HT(1D) and 5-HT(2A)) receptors, synephrine may be one of the important components in the constrictive effects of Evodiae Fructus.
authors.affiliation: Tsumura Research Laboratories, Tsumura & Co., Japan. hibini_tomoko@mail.tsumura.co.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hibino
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yuzurihara
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kase
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takeda
mesh_terms: D018674:Adrenergic Antagonists / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D032086:Evodia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012702:Serotonin Antagonists / Q000494:pharmacology*; D013578:Synephrine / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D018674:Adrenergic Antagonists; D004365:Drugs, Chinese Herbal; D012702:Serotonin Antagonists; D013578:Synephrine