title: Novel SNPs of the bovine NUCB2 gene and their association with growth traits in three native Chinese cattle breeds.
abstract: In this study, polymorphism in the exon 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 of bovine NUCB2 gene was detected by PCR-SSCP and DNA sequencing methods in 686 individuals from three Chinese cattle breeds. Two haplotypes (M and N), three observed genotypes (MM, MN and NN) and two SNPs (NC_007313: g. 27451G>A, NC_007313: g. 27472T>C) were detected. The frequencies of haplotypes M and N in inland Chinese three breeds were 0.531-0.721 and 0.279-0.469 respectively. The studied showed that Nanyang, Jiaxian Red and Qinchuan cattle populations were in Hardy-Weinberg equilibrium at SNPs locus of NUCB2 gene (P > 0.05). Polymorphism of the NUCB2 gene was shown to be associated with growth traits in Qingchuan and Nanyang cattle breed. The linkage of two mutant sites in the bovine NUCB2 gene had significant effects on body length, body weight, heart girth, and average daily gain at 24 months (P < 0.05). Results of this study suggested that the NUCB2-gene-specific SNP may be a useful marker for growth traits in future marker-assisted selection programmes in inland Chinese cattle.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, People's Republic of China.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: C Z
authors.initials: CZ
authors.lastname: Lei
authors.forename: G
authors.initials: G
authors.lastname: Ren
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Li
authors.forename: J Q
authors.initials: JQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D004268:DNA-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins