title: Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy.
abstract: We develop a standardized, fully automated, quantification system for liver fibrosis assessment using second harmonic generation microscopy and a morphology-based quantification algorithm. Liver fibrosis is associated with an abnormal increase in collagen as a result of chronic liver diseases. Histopathological scoring is the most commonly used method for liver fibrosis assessment, where a liver biopsy is stained and scored by experienced pathologists. Due to the intrinsic limited sensitivity and operator-dependent variations, there exist high inter- and intraobserver discrepancies. We validate our quantification system, Fibro-C-Index, with a comprehensive animal study and demonstrate its potential application in clinical diagnosis to reduce inter- and intraobserver discrepancies.
authors.affiliation: Institute of Bioengineering and Nanotechnology, The Nanos #04-01, 31 Biopolis Way, Singapore, 138669. dtai@ibn.a-star.edu.sg
authors.forename: Dean C S
authors.initials: DC
authors.lastname: Tai
authors.forename: Nancy
authors.initials: N
authors.lastname: Tan
authors.forename: Shuoyu
authors.initials: S
authors.lastname: Xu
authors.forename: Chiang Huen
authors.initials: CH
authors.lastname: Kang
authors.forename: Ser Mien
authors.initials: SM
authors.lastname: Chia
authors.forename: Chee Leong
authors.initials: CL
authors.lastname: Cheng
authors.forename: Aileen
authors.initials: A
authors.lastname: Wee
authors.forename: Chiang Li
authors.initials: CL
authors.lastname: Wei
authors.forename: Anju Mythreyi
authors.initials: AM
authors.lastname: Raja
authors.forename: Guangfa
authors.initials: G
authors.lastname: Xiao
authors.forename: Shi
authors.initials: S
authors.lastname: Chang
authors.forename: Jagath C
authors.initials: JC
authors.lastname: Rajapakse
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
authors.forename: Hui-Huan
authors.initials: HH
authors.lastname: Tang
authors.forename: Chien Shing
authors.initials: CS
authors.lastname: Chen
authors.forename: Hanry
authors.initials: H
authors.lastname: Yu
mesh_terms: D000465:Algorithms; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods*; D008103:Liver Cirrhosis / Q000473:pathology*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity