title: Neuralized-2: expression in human and rodents and interaction with Delta-like ligands.
abstract: Delta-Notch signaling is a universal cell-cell communication pathway crucial for numerous developmental and physiological processes. Several proteins interact with and regulate the Notch pathway, including the E3 ubiquitin ligase Neuralized (Neur) that influences the stability and activity of Notch ligands. In mammals there are two homologs of Neur, Neur1 and Neur2, that both can interact with Notch ligands Delta-like1 and Jagged1. Here, we show that Neur2, in contrast to Neur1, is highly expressed during embryonic development of the brain and several non-neural tissues and its mRNA levels subside postnatally. In the hippocampal neurons of the adult brain Neur2 transcripts, in contrast to Neur1, are excluded from the dendrites. Neur2 protein has a predominantly cytoplasmic localization. We also show that in addition to Delta-like1, Neur1 and Neur2 interact with another Notch ligand, Delta-like4.
authors.affiliation: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Grete
authors.initials: G
authors.lastname: Rullinkov
authors.forename: Richard
authors.initials: R
authors.lastname: Tamme
authors.forename: Anzelika
authors.initials: A
authors.lastname: Sarapuu
authors.forename: Juha
authors.initials: J
authors.lastname: Laurén
authors.forename: Mari
authors.initials: M
authors.lastname: Sepp
authors.forename: Kaia
authors.initials: K
authors.lastname: Palm
authors.forename: Tõnis
authors.initials: T
authors.lastname: Timmusk
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000201:enzymology; D002135:Calcium-Binding Proteins; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008024:Ligands; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology; D051381:Rats; D014158:Transcription, Genetic; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; C424126:DLL4 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D008565:Membrane Proteins; D009124:Muscle Proteins; C483082:NEURL2 protein, human; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases