Proteome analysis of biomarkers in the cerebrospinal fluid of neuromyelitis optica patients.
OBJECTIVE
To better understand the pathophysiological mechanisms underlying neuromyelitis optica (NMO), we developed a proteomics platform for biomarker discovery in the cerebrospinal fluid (CSF) of patients with NMO.


METHODS
Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) were used to compare the CSF proteome of NMO patients with that of controls. A subsequent ELISA and western blot analysis were performed to verify the results of the proteomic analysis. Pathway Studio 5.0 software was used to determine possible functional interactions among these differentially expressed proteins.


RESULTS
Using 2-DE and MALDI-TOF MS, we identified 11 differentially expressed proteins and two isoforms of these same proteins. The expression of four proteins was enhanced, whereas the expression of seven proteins was reduced in the NMO group in comparison to the control group. These differences in protein expression were confirmed by performing ELISA and western blot analyses (p<0.01). Protein network analyses revealed biologic interactions and cross-talks among these differentially expressed proteins.


CONCLUSIONS
Because of their unique expression profile in NMO CSFs, these proteins are candidate biomarkers for NMO. Thus, our findings may have important implications for both the diagnosis of NMO and the further understanding of its pathogenesis.
Bai
Shumei
S
Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Shandong 250012, People's Republic of China.
Liu
Shilian
S
Guo
Xuxiao
X
Qin
Zhaoyu
Z
Wang
Banqin
B
Li
Xiaohong
X
Qin
Yanjiang
Y
Liu
Yi-Hsin
YH
D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009471:Neuromyelitis Optica / Q000134:cerebrospinal fluid*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D015203:Reproducibility of Results; D014809:Vitamin D-Binding Protein / Q000378:metabolism
D015415:Biomarkers; D006242:Haptoglobins; D010455:Peptides; D020033:Protein Isoforms; D020543:Proteome; D014809:Vitamin D-Binding Protein