title: [Effects of FAK expression level on proliferation and motility of colorectal carcinoma cells].
abstract: OBJECTIVE
  To investigate the effect of siRNA targeting FAK gene on proliferation and motility of colorectal cancer.
  
  
  METHODS
  Recombinant plasmids that produced siRNAs targeting FAK were designed and cloned, then transfected into Caco-2 cells. The changes of FAK expression levels were examined by RT-PCR and immunocytochemistry. The effects of FAK gene knockdown on apoptotic morphological changes, proliferation, and motility were investigated.
  
  
  RESULTS
  Recombinant plasmids targeting FAK were successfully constructed, FAK mRNA and protein level was silenced in Caco-2 cells significantly. The inhibition of mRNA level was achieved maximal at 48 h post transfection with time dependent. The ability of proliferation and motility of Caco-2 cells were significantly decreased.
  
  
  CONCLUSIONS
  Plasmid-mediated FAK siRNA could inhibit FAK gene expression. The proliferation, motility and apoptosis were inhibited effectively, which suggested that FAK expression is closely associated with the proliferation, motility and apoptosis, etc. The results may be used as the reference for gene therapy of colorectal cancer.
authors.affiliation: Lab of Tumor Cellular and Molecular Biology, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China. pnpanpan07@126.com
authors.forename: Ning
authors.initials: N
authors.lastname: Pan
authors.forename: Ai-ran
authors.initials: AR
authors.lastname: Zhang
authors.forename: Mao-sen
authors.initials: MS
authors.lastname: Mu
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Hou
mesh_terms: D017209:Apoptosis; D018938:Caco-2 Cells; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D051417:Focal Adhesion Kinase 1 / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human