title: Flow cytometry discrimination between bacteria and clay-humic acid particles during growth-linked biodegradation of phenanthrene by Pseudomonas aeruginosa 19SJ.
abstract: Abstract Methods that quickly assess microbial density and aggregation in soil and sediments are needed in environmental microbiology. We report a flow cytometry method that uses the green and orange emission of the fluorochrome SYTO-13 to discriminate between bacteria and clay-humic acid particles. This approach distinguishes single or clustered bacteria, and clusters of bacteria and abiotic particles during the growth of the biosurfactant-producing strain Pseudomonas aeruginosa 19SJ on solid phenanthrene in the presence of humic acid-clay complexes.
authors.affiliation: Departament de Microbiologia, Universitat de Barcelona, Avenida Diagonal 645, 08028 Barcelona, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Resina-Pelfort
authors.forename: Marta
authors.initials: M
authors.lastname: García-Junco
authors.forename: José Julio
authors.initials: JJ
authors.lastname: Ortega-Calvo
authors.forename: Jaume
authors.initials: J
authors.lastname: Comas-Riu
authors.forename: Josep
authors.initials: J
authors.lastname: Vives-Rego