title: Study of peak shape and efficiency in butyl acrylate-based monolithic columns for capillary electrochromatography.
abstract: The study and modelling peak shape of in capillary electrochromatography (CEC), obtained using butyl acrylate (BA)-based monolithic columns, is described in this article. A modified-Gaussian model, which is a function of conventional experimental parameters: retention time (t(R)), peak height (H(0)) and standard deviation (sigma(0)) at the peak maximum, and left (A) and right (B) halfwidths, was used to describe the peaks of thiourea and several polyaromatic hydrocarbons compounds, which were eluted under several applied voltages. A mean relative error below 2% in the prediction of peak shape is obtained. Based on peak parameters, an easy and reliable estimation of global chromatographic performance, efficiency and peak capacity of BA-based monoliths was also considered. A comparison in terms of chromatographic performance of BA-based monoliths in CEC with CE mode and RPLC microparticulate columns was also performed.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, 46100 Burjassot, Valencia, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Cantó-Mirapeix
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Herrero-Martínez
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Baeza-Baeza
authors.forename: Ernesto F
authors.initials: EF
authors.lastname: Simó-Alfonso
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D053801:Capillary Electrochromatography / Q000379:methods*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry; D013890:Thiourea / Q000737:chemistry
chemical_list: D000179:Acrylates; D011084:Polycyclic Aromatic Hydrocarbons; C032490:n-butyl acrylate; D013890:Thiourea