Somatic cell nuclear transfer in zebrafish.
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.
Siripattarapravat
Kannika
K
Comparative Medicine and Integrative Biology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.
Pinmee
Boonya
B
Venta
Patrick J
PJ
Chang
Chia-Cheng
CC
Cibelli
Jose B
JB
D000818:Animals; D005818:Genetic Engineering / Q000379:methods*; D006822:Hybrid Cells / Q000637:transplantation*; D053652:Nuclear Transfer Techniques; D015027:Zebrafish / Q000033:anatomy & histology* / Q000235:genetics*