Protective effect of lecithinized SOD on reactive oxygen species-induced xerostomia.
Reactive oxygen species (ROS) are believed to be involved in radiation-induced xerostomia, and the application of antioxidants may be a promising method for treating patients suffering from salivary gland dysfunction. In this study, we examined the ability of the antioxidant superoxide dismutase (SOD) to restore radiation-induced salivary gland dysfunction using a mouse model of radiation-induced salivary gland hypofunction and ultraviolet B (UVB)-irradiated human salivary gland cells. We administered lecithinized SOD (PC-SOD) prior to and after irradiation and measured the amount of saliva secreted. To confirm ROS generation, flow cytometry was performed using an oxidant-sensitive fluorescent dye, dihydroethidium, and CM-H(2)DCFDA. While no significant decrease in saliva secretion was observed after irradiation in the mice that were treated with PC-SOD, a significant reduction in saliva secretion was noted in the irradiated mice that were not treated with PC-SOD. Furthermore, flow cytometry clearly revealed that PC-SOD eliminated superoxide (O(2)(-)) induced by UVB radiation. These results suggested that PC-SOD may protect against exocrine gland dysfunction induced by radiation, presumably by rapidly converting O(2)(-) to hydrogen peroxide. We believe that our results may advance the potential application of antioxidants for the prevention of ROS-induced xerostomia.
Tai
Yoshinori
Y
Department of Geriatric Dentistry, Tsurumi University School of Dental Medicine, Yokohama, Japan.
Inoue
Hiroko
H
Sakurai
Takashi
T
Yamada
Hiroyuki
H
Morito
Mitsuhiko
M
Ide
Fumio
F
Mishima
Kenji
K
Saito
Ichiro
I
D000818:Animals; D001610:Beta Particles; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D051379:Mice; D008810:Mice, Inbred C57BL; D010713:Phosphatidylcholines / Q000008:administration & dosage*; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000009:adverse effects*; D012463:Saliva / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000008:administration & dosage*; D014987:Xerostomia / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control*
D010713:Phosphatidylcholines; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; C434045:lecithinized superoxide dismutase