title: Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering.
abstract: We present real-time, rapid detection of Mycoplasma pneumonia in PBS inside a Y-channel PDMS microfluidic device via optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed with serially diluted M. pneumonia solutions using highly carboxylated polystyrene particles of 390 and 500 nm diameter conjugated with monoclonal anti-M. pneumonia. Proximity optical fibers were located around the viewing cell of the device, which were used to measure the increase in 45 degrees forward light scattering of the aggregated particles. The detection limit are slightly less than 50 pg mL(-1) both for 390 and 500 nm microspheres and the detection time do not exceed 90 s.
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Keesung
authors.initials: K
authors.lastname: Kim
authors.forename: Ho-Sup
authors.initials: HS
authors.lastname: Jung
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Man-Ryul
authors.initials: MR
authors.lastname: Lee
authors.forename: Kye-Seong
authors.initials: KS
authors.lastname: Kim
authors.forename: Kahp-Yang
authors.initials: KY
authors.lastname: Suh
mesh_terms: D055910:Antibodies, Immobilized / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018929:Cell Culture Techniques; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005336:Fiber Optic Technology; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007841:Latex Fixation Tests / Q000295:instrumentation / Q000379:methods*; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification* / Q000378:metabolism; D009757:Nylons / Q000737:chemistry; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer