title: Treadmill training enhances axon regeneration in injured mouse peripheral nerves without increased loss of topographic specificity.
abstract: We investigated the extent of misdirection of regenerating axons when that regeneration was enhanced by using treadmill training. Retrograde fluorescent tracers were applied to the cut proximal stumps of the tibial and common fibular nerves 2 or 4 weeks after transection and surgical repair of the mouse sciatic nerve. The spatial locations of retrogradely labeled motoneurons were studied in untreated control mice and in mice receiving 2 weeks of treadmill training, according to either a continuous protocol (10 m/minute, 1 hour/day, 5 days/week) or an interval protocol (20 m/minute for 2 minutes, followed by a 5-minute rest, repeated four times, 5 days/week). More retrogradely labeled motoneurons were found in both treadmill-trained groups. The magnitude of this increase was as great as or greater than that found after using other enhancement strategies. In both treadmill-trained groups, the proportions of motoneurons labeled from tracer applied to the common fibular nerve that were found in spinal cord locations reserved for tibial motoneurons in intact mice were no greater than in untreated control mice and significantly less than those found after electrical stimulation or chondroitinase treatment. Treadmill training in the first 2 weeks following peripheral nerve injury produces a marked enhancement of motor axon regeneration without increasing the propensity of those axons to choose pathways leading to functionally inappropriate targets.
authors.affiliation: Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA. art@cellbio.emory.edu
authors.forename: Arthur W
authors.initials: AW
authors.lastname: English
authors.forename: Delia
authors.initials: D
authors.lastname: Cucoranu
authors.forename: Amanda
authors.initials: A
authors.lastname: Mulligan
authors.forename: Manning
authors.initials: M
authors.lastname: Sabatier
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019765:Chondroitin ABC Lyase / Q000378:metabolism; D003911:Dextrans / Q000378:metabolism; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000379:methods; D005080:Exercise Test / Q000379:methods; D005081:Exercise Therapy / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009046:Motor Neurons / Q000473:pathology / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D020426:Sciatic Neuropathy / Q000503:physiopathology* / Q000534:rehabilitation*; D013116:Spinal Cord / Q000473:pathology; D013997:Time Factors
chemical_list: D003911:Dextrans; D019765:Chondroitin ABC Lyase