title: Development of monoclonal antibodies for analyzing immune and hematopoietic systems of common marmoset.
abstract: OBJECTIVE
  Common marmosets are considered experimental animals of primates useful for medical research. We developed several monoclonal antibodies (mAbs) directed to CD molecules to gain initial insight into the immune and hematopoietic systems of this organism, and analyzed the basic cellularity and characters of marmoset lymphocytes.
  
  
  METHODS
  Anti-marmoset CD antigen mAbs were prepared using marmoset antigen-expressing transfectants and used for flow cytometric analyses and cell fractionation. Expression of T-cell-related cytokine gene transcripts was examined in response to T-cell receptor stimulation by reverse transcription polymerase chain reaction analyses. Hematopoietic progenitor activities of marmoset bone marrow cells were examined in fractionated cells by mAbs against CD117 (c-kit) and CD34.
  
  
  RESULTS
  CD4 and CD8 expression profiles in T-cell subsets of marmoset were essentially similar to those in mouse and human. CD4(+) and CD8(+) subsets were isolated from marmoset spleens. Detected transcripts after stimulation of T cells included Th1-, Th2-, and Th17-related cytokines in CD4(+) cells and cytotoxic proteases in CD8(+) cells, respectively. Colony-forming abilities were detected mainly in CD117 (c-kit)(+) cells, irrespective of CD34 expression.
  
  
  CONCLUSIONS
  Marmoset immune system was basically similar to human and mouse systems.
authors.affiliation: Department of Immunology, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kametani
authors.forename: Daisuke
authors.initials: D
authors.lastname: Suzuki
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kohu
authors.forename: Masanobu
authors.initials: M
authors.lastname: Satake
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemizu
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.forename: Toshio
authors.initials: T
authors.lastname: Ito
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tamaoki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Mizushima
authors.forename: Manabu
authors.initials: M
authors.lastname: Ozawa
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Kito
authors.forename: Hideo
authors.initials: H
authors.lastname: Arai
authors.forename: Akemi
authors.initials: A
authors.lastname: Koyanagi
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Sonoko
authors.initials: S
authors.lastname: Habu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D016466:CHO Cells; D002144:Callithrix / Q000097:blood / Q000276:immunology* / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D053804:Granzymes / Q000235:genetics; D006410:Hematopoiesis / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007107:Immune System / Q000276:immunology*; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D053804:Granzymes