Anopheles gambiae TRPA1 is a heat-activated channel expressed in thermosensitive sensilla of female antennae.
Heat sensitivity is a sensory modality that plays a critical role in close-range host-seeking behaviors of adult female Anopheles gambiae, the principal Afrotropical vector for human malaria. An essential step in this activity is the ability to discriminate and respond to increases in environmental temperature gradients through the process of peripheral thermoreception. Here, we report on the characterization of the anopheline homolog of the transient receptor potential (TRP) A1/ANKTM1 channel that is consistent with its role as a heat-sensor in host-seeking adult female mosquitoes. We identify a set of distal antennal sensory structures that specifically respond to temperature gradients and express AgTRPA1. Functional characterization of AgTRPA1 in Xenopus oocytes supports its role in the molecular transduction of temperature gradients in An. gambiae, providing a basis for targeting mosquito heat responses as a means toward reducing malaria transmission.
Wang
Guirong
G
Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.
Qiu
Yu T
YT
Lu
Tan
T
Kwon
Hyung-Wook
HW
Pitts
R Jason
RJ
Van Loon
Joop J A
JJ
Takken
Willem
W
Zwiebel
Laurence J
LJ
D000818:Animals; D000852:Anopheles / Q000378:metabolism*; D004594:Electrophysiology; D005260:Female; D005456:Fluorescent Dyes; D006358:Hot Temperature; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011984:Sensory Receptor Cells / Q000378:metabolism*; D012815:Signal Processing, Computer-Assisted; D050051:Transient Receptor Potential Channels / Q000235:genetics / Q000378:metabolism*
D005456:Fluorescent Dyes; D012333:RNA, Messenger; D050051:Transient Receptor Potential Channels