title: [Identification of differentially expressed genes in myocardium of patients with heart failure by human whole genomic oligonucleotide microarray-assisted pathways analysis].
abstract: OBJECTIVE
  To identify the differentially expressed gene profiles in myocardium of patients with heart failure using human whole genomic oligonucleotide microarray-assisted pathway analysis.
  
  
  METHODS
  Phalanx whole genomic oligonucleotide microarrays were used to detect the gene expression profiles of myocardium in four patients died of heart failure and 4 brain died patients without heart diseases. The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The genes with a threshold of 1.2 times fold-change were selected and BioCarta Pathway and KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway databases were used to identify functionally related gene pathways.
  
  
  RESULTS
  A total of 2806 genes with differentially expression were detected between the failing and non-failing heart samples, expression changes of 399 genes were more than 2-folds. Eleven pathways were identified by BioCarta pathway database and sixteen pathways were identified by KEGG PATHWAY Database.
  
  
  CONCLUSIONS
  Genomic microarray-assisted pathway analysis could help to identify gene expression profiles in failing heart.
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Wan
authors.forename: Hong-Jin
authors.initials: HJ
authors.lastname: Wu
authors.forename: Guang
authors.initials: G
authors.lastname: Zhi
authors.forename: Cang-Song
authors.initials: CS
authors.lastname: Xiao
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Gao
authors.forename: Jia-Jin
authors.initials: JJ
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D020869:Gene Expression Profiling; D005796:Genes; D015894:Genome, Human; D055106:Genome-Wide Association Study; D005838:Genotype; D006333:Heart Failure / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
chemical_list: D012313:RNA