A highly branched 1,3-beta-D-glucan extracted from Aureobasidium pullulans induces cytokine production in DBA/2 mouse-derived splenocytes.
We recently elucidated the structure of a highly branched 1,3-beta-D-glucan with 6-monoglucopyranosyl side chains, extracted from Aureobasidium pullulans (AP-FBG). Although the biological effects of beta-D-glucans are known to depend on their structures, the effects of a highly branched 1,3-beta-D-glucan on the production of cytokines by leukocytes in mice have not yet been elucidated. In this study, we found that AP-FBG strongly induced the production of various cytokines, especially Th1 cytokines (e.g., IFN-gamma and IL-12p70) and Th17 cytokines (e.g., IL-17A), but did not induce the production of IL-4, IL-10, and TNF-alpha in DBA/2 mouse-derived splenocytes in vitro.
Tada
Rui
R
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan.
Yoshikawa
Masashi
M
Kuge
Takao
T
Tanioka
Asuka
A
Ishibashi
Ken-ichi
K
Adachi
Yoshiyuki
Y
Tsubaki
Kazufumi
K
Ohno
Naohito
N
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005658:Fungi / Q000737:chemistry; D007114:Immunization; D007239:Infections / Q000276:immunology / Q000628:therapy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D047071:beta-Glucans / Q000008:administration & dosage*
D016207:Cytokines; D047071:beta-Glucans; C033363:beta-1,3-glucan