title: Clostridium difficile infections among Jordanian adult hospitalized patients.
abstract: This prospective study investigated the important epidemiologic aspects of Clostridium difficile infections (CDIs) among Jordanian adult hospitalized patients. A total of 300 stool specimens were investigated using culture and polymerase chain reaction methods for detection of C difficile, its toxins, and fluoroquinolone resistance. C difficile-positive cultures were found in 13.7% of the patients, and 73% of the isolates carried tcdA and/or tcdB toxin genes, and all C difficile isolates were negative for binary toxin. The isolates showed moderate level of resistance to both ciprofloxacin and levofloxacin, whereas metronidazole and vancomycin were highly susceptible. This study indicates the need for early detection of CDIs and prevention of its severe disease in hospitalized patients.
authors.affiliation: Department of Pathology-Microbiology, Faculty of Medicine, University of Jordan, Amman 11942, Jordan.
authors.forename: Lina M
authors.initials: LM
authors.lastname: Nasereddin
authors.forename: Fares G
authors.initials: FG
authors.lastname: Bakri
authors.forename: Asem A
authors.initials: AA
authors.lastname: Shehabi
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D001431:Bacteriological Techniques; D016360:Clostridioides difficile / Q000187:drug effects / Q000302:isolation & purification*; D004761:Enterocolitis, Pseudomembranous / Q000453:epidemiology*; D004768:Enterotoxins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology; D006761:Hospitals; D006801:Humans; D007597:Jordan; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D024841:Fluoroquinolones; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile