Single-feature polymorphism discovery by computing probe affinity shape powers.
BACKGROUND
Single-feature polymorphism (SFP) discovery is a rapid and cost-effective approach to identify DNA polymorphisms. However, high false positive rates and/or low sensitivity are prevalent in previously described SFP detection methods. This work presents a new computing method for SFP discovery.


RESULTS
The probe affinity differences and affinity shape powers formed by the neighboring probes in each probe set were computed into SFP weight scores. This method was validated by known sequence information and was comprehensively compared with previously-reported methods using the same datasets. A web application using this algorithm has been implemented for SFP detection. Using this method, we identified 364 SFPs in a barley near-isogenic line pair carrying either the wild type or the mutant uniculm2 (cul2) allele. Most of the SFP polymorphisms were identified on chromosome 6H in the vicinity of the Cul2 locus.


CONCLUSIONS
This SFP discovery method exhibits better performance in specificity and sensitivity over previously-reported methods. It can be used for other organisms for which GeneChip technology is available. The web-based tool will facilitate SFP discovery. The 364 SFPs discovered in a barley near-isogenic line pair provide a set of genetic markers for fine mapping and future map-based cloning of the Cul2 locus.
Xu
Wayne Wenzhong
WW
Supercomputing Institute for Advanced Computational Research, University of Minnesota, Minnesota, MN 55455, USA. wxu@msi.umn.edu
Cho
Seungho
S
Yang
S Samuel
SS
Bolon
Yung-Tsi
YT
Bilgic
Hatice
H
Jia
Haiyan
H
Xiong
Yanwen
Y
Muehlbauer
Gary J
GJ
D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D015342:DNA Probes / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D001467:Hordeum / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
D015342:DNA Probes; D018744:DNA, Plant