Bacterial genetic methods to explore the biology of mariner transposons.
Mariners are small DNA mediated transposons of eukaryotes that fortuitously function in bacteria. Using bacterial genetics, it is possible to study a variety of properties of mariners, including transpositional ability, dominant-negative regulation, overexpresson inhibition, and the function of cis-acting sequences like the inverted terminal repeats. In conjunction with biochemical techniques, the structure of the transposase can be elucidated and the activity of the elements can be improved for genetic tool use. Finally, it is possible to uncover functional transposase genes directly from genomes given a suitable bacterial genetic screen.
Lampe
David J
DJ
Department of Biological Sciences, Duquesne University, 600 Forbes Ave., Pittsburgh, PA 15116, USA. lampe@duq.edu
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D056890:Eukaryota; D018014:Gene Transfer Techniques; D005821:Genetic Techniques; D015316:Genetic Therapy / Q000379:methods; D016680:Genome, Bacterial; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D018626:Retroelements; D019895:Transposases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D018626:Retroelements; C111652:mariner transposases; D019895:Transposases