title: Antioxidative and antigenotoxic effects of garlic (Allium sativum L.) prepared by different processing methods.
abstract: This study describes the antioxidant activities and antigenotoxic effects of garlic extracts prepared by different processing methods. Aged-garlic extract (AGE) showed a significantly higher total phenolic content (562.6 +/- 1.92 mg/100 g garlic acid equivalents) than those of raw garlic extract (RGE) or heated garlic extract (HGE). The SC(50) for DPPH RSA in HGE was significantly the highest at 2.1 mg/ml. The SC(50) for SOD-like activity in garlic extracts was, in decreasing order, RGE (7.3 mg/ml) > AGE (8.5 mg/ml) > HGE (9.2 mg /ml). The ED(50) of AGE was the highest (19.3 microg/ml) regarding H2O2 induced DNA damage and its inhibition rate was 70.8%. The ED(50) of RGE for 4-hydroxynonenal (a lipid peroxidation product) induced DNA damage was 38.6 microg/ml, followed by AGE > HGE. Although the heat treatment of garlic tended to decrease the TPC and SOD-like activity and increased DPPH RSA, garlic, in general, has significant antioxidant activity and protective effects against oxidative DNA damage regardless of processing method.
authors.affiliation: Department of Food and Nutrition, Kyungnam University, Masan 631-701, Republic of Korea.
authors.forename: Jae-Hee
authors.initials: JH
authors.lastname: Park
authors.forename: Yoo Kyoung
authors.initials: YK
authors.lastname: Park
authors.forename: Eunju
authors.initials: E
authors.lastname: Park
mesh_terms: D000447:Aldehydes; D016587:Antimutagenic Agents / Q000032:analysis / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001713:Biphenyl Compounds; D004249:DNA Damage / Q000187:drug effects*; D005511:Food Handling; D005737:Garlic / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D006861:Hydrogen Peroxide; D007962:Leukocytes / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase
chemical_list: D000447:Aldehydes; D016587:Antimutagenic Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase; C027576:4-hydroxy-2-nonenal