title: Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants: potential insights into biomarker development.
abstract: Liver proteome response of largemouth bass (Micropterus salmoides) exposed to environmental contaminants was analyzed to identify novel biomarkers of exposure. Adult male bass were exposed to cadmium chloride (CdCl(2)), atrazine, PCB 126, phenanthrene, or toxaphene via intraperitoneal injection with target body burdens of 0.00067, 3.0, 2.5, 50, and 100 microg/g, respectively. After a 96 h exposure, hepatic proteins were separated with two-dimensional gel electrophoresis and differentially expressed proteins (vs. controls) recognized and identified with MALDI-TOF/TOF mass spectrometry. We identified, 30, 18, eight, 19, and five proteins as differentially expressed within the CdCl(2), atrazine, PCB 126, phenanthrene, and toxaphene treatments, respectively. Alterations were observed in the expression of proteins associated with cellular ion homeostasis (toxaphene), oxidative stress (phenanthrene, PCB 126), and energy production including glycolysis (CdCl(2), atrazine) and ATP synthesis (atrazine). This work supports the further evaluation of several of these proteins as biomarkers of contaminant exposure in fish.
authors.affiliation: Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN 47907, USA. bcsanche@purdue.edu
authors.forename: Brian C
authors.initials: BC
authors.lastname: Sanchez
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Ralston-Hooper
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Kowalski
authors.forename: H
authors.initials: H
authors.lastname: Dorota Inerowicz
authors.forename: Jiri
authors.initials: J
authors.lastname: Adamec
authors.forename: María S
authors.initials: MS
authors.lastname: Sepúlveda
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism / Q000502:physiology*; D015415:Biomarkers / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004781:Environmental Exposure; D008099:Liver / Q000187:drug effects*; D008297:Male; D040901:Proteomics; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical