title: High-speed cell sorting of infectious trophic and cystic forms of Pneumocystis carinii.
abstract: The separation of Pneumocystis carinii life-cycle stages while preserving infectivity is a hitherto unresolved challenge. We describe an original, reproducible, and efficient method for separating trophic from cystic forms of P. carinii using a high-speed cell sorter. The large amounts of highly purified (99.6+/-0.3%) infectious trophic and cystic forms can now be used to elucidate the poorly understood P. carinii life cycle.
authors.affiliation: Department of Parasitology, Lille Nord de France University, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Martinez
authors.forename: El Moukhtar
authors.initials: el M
authors.lastname: Aliouat
authors.forename: Muriel
authors.initials: M
authors.lastname: Pottier
authors.forename: Nausicâa
authors.initials: N
authors.lastname: Gantois
authors.forename: Claire
authors.initials: C
authors.lastname: Pinçon
authors.forename: Annie
authors.initials: A
authors.lastname: Standaert-Vitse
authors.forename: Eduardo
authors.initials: E
authors.lastname: Dei-Cas
authors.forename: Cécile-Marie
authors.initials: CM
authors.lastname: Aliouat-Denis
mesh_terms: D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D045363:Pneumocystis carinii / Q000145:classification* / Q000302:isolation & purification*