Construction of a recombinant autolytic wine yeast strain overexpressing the csc1-1 allele.
During the aging step of sparkling wines and wines aged on lees, yeast cells kept in contact with the wine finally die and undergo autolysis, releasing cellular compounds with a positive effect on the wine quality. In view of the interest of autolysis for wine properties, biotechnologists have tried to improve autolytic yield during winemaking. In this work we used genetic engineering techniques to construct an autolytic industrial strain by expressing the csc1-1 allele from the RDN1 locus. The expression of this mutant allele, that causes a "constitutive in autophagy phenotype," resulted in accelerated autolysis of the recombinant strain. Although autophagic phenotype due to csc1-1 expression has been reported to require the mutant allele in multicopy, autolytic acceleration was achieved by expressing only one or two copies of the gene under the control of the constitutive promotor pTDH3. The acceleration of autolysis together with the unaltered fermentative capacity, strongly supported the overexpression of csc1-1 allele as a strategy to obtain wines with aged-like properties in a shortened time.
Cebollero
Eduardo
E
Department of Microbiology, Instituto de Fermentaciones Industriales (CSIC), C. Juan de la Cierva 3, Madrid 28006, Spain. e.cebollero@umcutrecht.nl
Gonzalez-Ramos
Daniel
D
Gonzalez
Ramon
R
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001329:Autolysis / Q000235:genetics*; D001343:Autophagy / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D005818:Genetic Engineering / Q000379:methods*; D016681:Genome, Fungal; D042822:Genomic Instability; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014920:Wine / Q000382:microbiology*
D000596:Amino Acids; D056827:Endosomal Sorting Complexes Required for Transport; D029701:Saccharomyces cerevisiae Proteins; C107528:VPS4 protein, S cerevisiae; D000251:Adenosine Triphosphatases