Target deletion of the cytoskeleton-associated protein palladin does not impair neurite outgrowth in mice.
Palladin is an actin cytoskeleton-associated protein which is crucial for cell morphogenesis and motility. Previous studies have shown that palladin is localized to the axonal growth cone in neurons and may play an important role in axonal extension. Previously, we have generated palladin knockout mice which display cranial neural tube closure defect and embryonic lethality before embryonic day 15.5 (E15.5). To further study the role of palladin in the developing nervous system, we examined the innervation of palladin-deficient mouse embryos since the 200 kd, 140 kd, 90-92 kd and 50 kd palladin isoforms were undetectable in the mutant mouse embryo brain. Contrary to the results of previous studies, we found no inhibition of the axonal extension in palladin-deficient mouse embryos. The cortical neurons derived from palladin-deficient mice also showed no significant difference in neurite outgrowth as compared with those from wild-type mice. Moreover, no difference was found in neurite outgrowth of neural stem cell derived-neurons between palladin-deficient mice and wild-type mice. In conclusion, these results suggest that palladin is dispensable for normal neurite outgrowth in mice.
Shu
Run-Zhe
RZ
Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Zhang
Feng
F
Liu
Xue-Song
XS
Li
Chun-Liang
CL
Wang
Long
L
Tai
Yi-Lin
YL
Wu
Xiao-Lin
XL
Yang
Xue
X
Liao
Xiao-Dong
XD
Jin
Ying
Y
Gu
Ming-Min
MM
Huang
Lei
L
Pang
Xiao-Fen
XF
Wang
Zhu-Gang
ZG
D000818:Animals; D001921:Brain / Q000196:embryology; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D009420:Nervous System / Q000196:embryology; D016501:Neurites / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D020033:Protein Isoforms; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors
D003598:Cytoskeletal Proteins; D010750:Phosphoproteins; D020033:Protein Isoforms; C485873:palladin protein, mouse