Development of an Ibuprofen-releasing biodegradable PLA/PGA electrospun scaffold for tissue regeneration.
Our aim was to develop a biodegradable fibrous dressing to act as a tissue guide for in situ wound repair while releasing Ibuprofen to reduce inflammation in wounds and reduce pain for patients on dressing changes. Dissolving the acid form of Ibuprofen (from 1% to 10% by weight) in the same solvent as 75% polylactide, 25% polyglycolide (PLGA) polymers gave uniformly loaded electrospun fibers which gave rapid release of drug within the first 8 h and then slower release over several days. Scaffolds with 10% Ibuprofen degraded within 6 days. The Ibuprofen released from these scaffolds significantly reduced the response of fibroblasts to major pro-inflammatory stimulators. Fibroblast attachment and proliferation on scaffolds was unaffected by the addition of 1-5% Ibuprofen. Scaffolds loaded with 10% Ibuprofen initially showed reduced cell attachment but this was restored by soaking scaffolds in media for 24 h. In summary, addition of Ibuprofen to electrospun biodegradable scaffolds can give acute protection of adjacent cells to inflammation while the scaffolds provide an open 3D fibrous network to which cells can attach and migrate. By 6 days, such scaffolds will have completely dissolved into the wound bed obviating any need for dressing removal.
Cantón
Irene
I
Department of Engineering Materials, University of Sheffield, Kroto Research Institute, UK.
Mckean
Robert
R
Charnley
Mirren
M
Blackwood
Keith A
KA
Fiorica
Calogero
C
Ryan
Anthony J
AJ
MacNeil
Sheila
S
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007052:Ibuprofen / Q000008:administration & dosage*; D015603:Keratinocytes / Q000166:cytology; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D011091:Polyesters; D011100:Polyglycolic Acid; C033616:poly(lactide); D007052:Ibuprofen