title: Time-course of the retinal nerve fibre layer degeneration after complete intra-orbital optic nerve transection or crush: a comparative study.
abstract: We examined qualitatively and quantitatively in adult rat retinas the temporal degeneration of the nerve fibre layer after intra-orbital optic nerve transection (IONT) or crush (IONC). Retinal ganglion cell (RGC) axons were identified by their heavy neurofilament subunit phosphorylated isoform (pNFH) expression. Optic nerve injury induces a progressive axonal degeneration which after IONT proceeds mainly with abnormal pNFH-accumulations in RCG axons and after IONC in RGCs somas and dendrites. Importantly, this aberrant pNFH-expression pattern starts earlier and is more dramatic after IONT than after IONC, highlighting the importance that the type of injury has on the time-course of RGC degeneration.
authors.affiliation: Laboratorio de Oftalmología Experimental, Facultad de Medicina, Universidad de Murcia, 30100 Murcia, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Parrilla-Reverter
authors.forename: Marta
authors.initials: M
authors.lastname: Agudo
authors.forename: Francisco
authors.initials: F
authors.lastname: Nadal-Nicolás
authors.forename: Luis
authors.initials: L
authors.lastname: Alarcón-Martínez
authors.forename: Manuel
authors.initials: M
authors.lastname: Jiménez-López
authors.forename: Manuel
authors.initials: M
authors.lastname: Salinas-Navarro
authors.forename: Paloma
authors.initials: P
authors.lastname: Sobrado-Calvo
authors.forename: José M
authors.initials: JM
authors.lastname: Bernal-Garro
authors.forename: María P
authors.initials: MP
authors.lastname: Villegas-Pérez
authors.forename: Manuel
authors.initials: M
authors.lastname: Vidal-Sanz
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D009409:Nerve Crush; D009412:Nerve Fibers / Q000378:metabolism / Q000473:pathology; D016900:Neurofilament Proteins / Q000378:metabolism; D020221:Optic Nerve Injuries / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012162:Retinal Degeneration / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000473:pathology*
chemical_list: D016900:Neurofilament Proteins