[Expression of ICOSL on human coronary artery endothelial cells and its being interferentialed by ox-LDL].
OBJECTIVE
To study the expression of inducible co-stimulator ligand(ICOSL) on human coronary artery endothelial cells(HCAEC) and its being interferentialed by oxidized low density lipoprotein(ox-LDL).


METHODS
ICOSL expression levels were determined by the fluorescence, reverse transcription PCR (RT-PCR) and Western blot, respectively.


RESULTS
The ICOSL mRNA OD values of control and 100 mg/L ox-LDL group was 0.071+/-0.035 and 0.186+/-0.044, respectively. The Western blot A values of control and 100 mg/L ox-LDL group was 10.88+/-1.53 and 16.03+/-4.08, respectively. ox-LDL increased expression of ICOSL mRNA and protein(P<0.05).


CONCLUSIONS
ICOSL can express on the HCAEC. ox-LDL can up-regulate the expression of ICOSL.
Xu
Lin
L
Department of Cardiology, Guangzhou General Hospital of Guangzhou Military Direct, Guangzhou 510010, China.
Li
Zhi-Liang
ZL
Zhu
Xiao-Xing
XX
Ma
Jun
J
Hong
Chang-Jiang
CJ
He
Jian-Xin
JX
Xiang
Ding-Cheng
DC
Qiu
Jian
J
D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand; D008077:Lipoproteins, LDL / Q000378:metabolism*; D015854:Up-Regulation
D015703:Antigens, CD; C498514:ICOSLG protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein