An alpaca single-domain antibody blocks filopodia formation by obstructing L-plastin-mediated F-actin bundling.
L-plastin, a conserved modular F-actin bundling protein, is ectopically expressed in tumor cells and contributes to cell malignancy and invasion. The underlying molecular mechanisms involved remain unclear, in part, because specific inhibitors of L-plastin are lacking. We used recombinant alpaca-derived L-plastin single-domain antibodies (nanobodies) as effector of L-plastin function in cells.
Delanote
Veerle
V
Department of Medical Protein Research, Flanders Institute for Biotechnology, Ghent, Belgium.
Vanloo
Berlinda
B
Catillon
Marie
M
Friederich
Evelyne
E
Vandekerckhove
Joël
J
Gettemans
Jan
J
D000199:Actins / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000915:Antibody Affinity; D002161:Camelids, New World / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000276:immunology / Q000502:physiology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000276:immunology / Q000503:physiopathology; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011554:Pseudopodia / Q000276:immunology* / Q000378:metabolism*; D034622:RNA Interference; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013816:Thermodynamics
D000199:Actins; D000906:Antibodies; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; C047262:LCP1 protein, human; D008840:Microfilament Proteins; D011994:Recombinant Proteins