title: Leukocyte elastase inhibitor: a new regulator of PARP-1.
abstract: Poly(ADP-ribose) polymerase-1 (PARP-1) uses NAD(+) as a substrate to form ADP-ribose. During apoptosis, caspases cleave PARP-1 to avoid excessive NAD consumption. Because PARP-1 is a key regulator of the activity of DNases involved in caspase-dependent apoptosis, its cleavage is required to promote DNA degradation. To explore the situation in caspase-independent cell death, we investigated the effect of PARP-1 on the acid endonuclease leukocyte elastase inhibitor (LEI)-derived DNase II (L-DNase II). We found for the first time an association between PARP-1 and LEI/L-DNase II. Unexpectedly, we observed that LEI influenced the automodification of PARP-1.
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Paris, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Leprêtre
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sidoli
authors.forename: A Ivana
authors.initials: AI
authors.lastname: Scovassi
authors.forename: Alicia
authors.initials: A
authors.lastname: Torriglia
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007150:Immunohistochemistry; D019272:Leukocyte Elastase / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013552:Swine
chemical_list: D011994:Recombinant Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II; D019272:Leukocyte Elastase