Pharmacological screening of bryophyte extracts that inhibit growth and induce abnormal phenotypes in human HeLa cancer cells.
Antitumor activities of substances from natural sources apart from vascular plants and micro-organisms have been poorly investigated. Here we report on a pharmacological screening of a bryophyte extract library using a phenotypic cell-based assay revealing microtubules, centrosomes and DNA. Among the 219 moss extracts tested, we identified 41 extracts acting on cell division with various combinations of significant effects on interphasic and mitotic cells. Seven extracts were further studied using a cell viability assay, cell cycle analysis and the phenotypic assay. Three distinct pharmacological patterns were identified including two unusual phenotypes.
Krzaczkowski
Lucie
L
Centre de Recherche en Pharmacologie approximately Santé, UMR 2587 CNRS-Pierre Fabre, ISTMT, 31432 Toulouse, Cedex 4, France.
Wright
Michel
M
Rebérioux
Delphine
D
Massiot
Georges
G
Etiévant
Chantal
C
Gairin
Jean Edouard
JE
D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D044002:Bryophyta / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018385:Centrosome / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D010936:Plant Extracts / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D004247:DNA