[Differentiation of recombinant adenoviruses expressed MDV-1 VP22 subcellular localization pattern].
OBJECTIVE
To study the transduction and localization mechanism of Marek's disease virus serotype 1 (MDV-1) CVI988 VP22 in different cells.


METHODS
VP22 was expressed with recombinant adenovirus and identified by immunofluorescence assay (IFA) and Western blot. Lysates of recombinant virus infected 293 cells were added to normal MDBK cells to identify the transduction property of VP22. AD-293 cells infected with recombinant virus were fixed to investigate localization of VP22 at different time post infection. Transient expression of VP22 in AD-293 cells was carried out for control.


RESULTS
The results showed that the VP22 expressed by recombinant adenovirus entered almost all the monolayer cells, which indicate the VP22 remains its transduction property. The VP22 first gather round the nucleus membrane, and then concentrated in particles in cytoplasm of 293 cells infected with recombinant adenovirus, compared with the nuclei localization pattern of VP22 in MDV infected CEF and transient expressed VP22 in 293 cells.


CONCLUSIONS
The VP22 presented a different localization pattern in cells infected with different recombinant virus.
Zhang
Chen-fei
CF
Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. zhangchenfei1981@163.com
Qin
Ai-jian
AJ
Chen
Juan-juan
JJ
Qian
Kun
K
DU
Jing
J
Yin
Tian-yan
TY
Shao
Hong-xia
HX
Jin
Wen-jie
WJ
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001717:Birds; D002417:Cattle; D002460:Cell Line; D003593:Cytoplasm / Q000821:virology; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008380:Marek Disease / Q000821:virology*; D009685:Nuclear Envelope / Q000821:virology; D021381:Protein Transport; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C450446:UL49 protein, Marek's disease virus type 1; D014764:Viral Proteins