Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy.
A novel aerobe thermophilic endospore-forming bacterium designated strain AF/04(T) was isolated from thermal mud located in Euganean hot springs, Abano Terme, Padova, Italy. Strain AF/04(T) was Gram-positive, motile, rod-shaped, occurring in pairs, or filamentous. The isolate grew between 55 and 67 degrees C (optimum 65 degrees C) and at pH 6.0-7.5 (optimum pH 7.2). The strain was aerobic and grew on maltose, trehalose, and sodium acetate as sole carbon sources. The G + C content of DNA was 53.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AF/04(T) falls within the genus Anoxybacillus. Levels of 16S rRNA gene sequence similarity between strain AF/04(T) and the type strains of recognized Anoxybacillus species ranged from 95 to 99%. Chemotaxonomic data (major isoprenoid quinone-menaquinone-7; major fatty acid iso-C15:0 and anteiso-C17:0) supported the affiliation of strain AF/04(T) to the genus Anoxybacillus. Based on phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA-DNA hybridization data, it was proposed that strain AF/04(T) (=DSM 17141(T) = ATCC BAA 1156(T)) should be placed in the genus Anoxybacillus as the type strain of a novel species, Anoxybacillus thermarum sp. nov.
Poli
Annarita
A
Istituto di Chimica Biomolecolare (ICB), CNR, Via Campi Flegrei 34, 80078, Pozzuoli (Naples), Italy. apoli@icb.cnr.it
Romano
Ida
I
Cordella
Paolo
P
Orlando
Pierangelo
P
Nicolaus
Barbara
B
Ceschi Berrini
Cristina
C
D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D045482:Hot Springs / Q000382:microbiology*; D007558:Italy; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S