1,3-Dichloro-2-propanol induces apoptosis via both calcium  and ROS in mouse melanoma cells.
We demonstrated that the apoptotic cell death of mouse melanoma cells exposed to 1,3- dichloro-2-propanol (DCP) was Ca(2+) dependent. 1,3-DCP inhibited the growth of mouse melanoma cells in a concentration-dependent manner. Furthermore, a terminal nick-end labeling of fragmented DNA (TUNEL) assay demonstrated that 1,3-DCP-induced apoptosis. Moreover, reactive oxygen species and Ca(2+), generated by mouse melanoma cells that were exposed to 1,3-DCP, resulted in the activation of NF-jB and MAPKs. Finally, treatment with BAPTA, an intracellular Ca(2+) chelator, completely inhibited the apoptosis that was induced by 1,3-DCP treatment of B16F10 cells.
Park
Sun Young
SY
Korea BIO-IT Foundry Center, Pusan National University, Busan, 609-735, Korea.
Kim
Young Hun
YH
Kim
Young Hee
YH
Lee
Sang-Joon
SJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000517:alpha-Chlorohydrin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D016328:NF-kappa B; D017382:Reactive Oxygen Species; C033489:1,3-dichloro-2-propanol; D000517:alpha-Chlorohydrin; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases; D002118:Calcium