Flow cytometric detection and serotyping of enterovirus for the clinical laboratory.
Culture and serotyping of human enteroviruses by fluorescence microscopy are time-consuming and labor-intensive. Flow cytometry has the potential of being more rapid, sensitive, and objective but has not been used for these purposes in a clinical laboratory. Primary rhesus monkey kidney (PMK) cells were inoculated with several enterovirus serotypes and stained with enterovirus-specific antibodies for flow cytometry and indirect fluorescence antibody testing (IFA). Kinetic studies of coxsackievirus B1 and echovirus 30 infection of PMK cells were performed on days 1-4 after inoculation. Flow cytometry results for echovirus 6, 9, 11, and 30 and coxsackievirus B1 correlated with IFA in all cases. Coxsackievirus B1 and echovirus 30 infections were detected 1 day earlier by flow cytometry than IFA. Flow cytometry can be effectively used for detecting enterovirus-infected cells in a clinical laboratory with the advantages of better quantitation of low levels of infection and earlier detection of virally infected cells in culture systems.
She
Rosemary C
RC
Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA. rosemary.she-bender@hsc.utah.edu
Preobrazhensky
Sergey N
SN
Taggart
Edward W
EW
Petti
Cathy A
CA
Bahler
David W
DW
D000818:Animals; D002478:Cells, Cultured; D004770:Enterovirus / Q000145:classification / Q000472:pathogenicity; D029822:Enterovirus B, Human / Q000145:classification / Q000472:pathogenicity; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000821:virology*; D007700:Kinetics; D008253:Macaca mulatta; D012703:Serotyping