[Pharmacokinetic study of polydatin in Beagle dogs].
OBJECTIVE
To establish a method for detecting plasma polydatin using high-performance liquid chromatography (HPLC) and investigate the pharmacokinetics of polydatin in Beagle dogs.


METHODS
HPLC-based detection of polydatin was performed on a Hypersil-BDS C18 column with a mobile phase of methanol and water (35:65) at the flow rate of 1.0 ml/min and the detection wavelength of 290 nm. The pharmacokinetic parameters of polydatin were calculated by non-compartment model statistics.


RESULTS
The standard curve was linear within the range of 0.21-21.0 microg/ml (correlation coefficient of 0.9995, n=5). The average recovery was 90% with a relative standard deviation no more than 8.0%. The limit of detection of the method was 0.1 microg/ml. The pharmacokinetic parameters of polydatin at 3 doses were obtained, with T((1/2)); of 89.95-/+19.96 min, 152.15-/+55.82 min, and 202.36-/+66.75 min, and AUC0-infinity of 300.14-/+51.33 g.min.ml(-1), 751.72-/+173.97 g.min.ml(-1) and 1521.12-/+310.02 microg.min.ml(-1), respectively.


CONCLUSIONS
The method we established allows effective detection of polydatin, whose pharmacokinetics conforms to the two-compartment model.
Cai
Zhuo
Z
Department of Pharmacy, 458 Hospital of PLA, Guangzhou 510602, China.
Yu
Fa
F
Ma
An-de
AD
Yang
Xue-mei
XM
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D013267:Stilbenes / Q000097:blood / Q000493:pharmacokinetics*
D005960:Glucosides; D013267:Stilbenes; C058229:polydatin