Detection of rotavirus in food associated with a gastroenteritis outbreak in a mother and child sanatorium.
In the course of a rotavirus outbreak in a mother and child sanatorium 74 food samples from the sanatorium kitchen were taken and tested for rotavirus. Rotavirus particles were isolated from 25 g food samples by a simple method including ultrafiltration, originally designed for the detection of norovirus in various food matrices. Rotavirus was successfully detected in a sample of potato stew by conventional RT-seminested-PCR. Sequence comparison of the amplification products obtained from the potato stew and a stool sample from an infected child verified that the two viruses were identical.
Mayr
Constanze
C
Chemisches und Veterinaeruntersuchungsamt Stuttgart, Schaflandstrasse 3/2, D-70736 Fellbach, Germany.
Strohe
Gerald
G
Contzen
Matthias
M
D001483:Base Sequence; D002648:Child; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology*; D005516:Food Microbiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000235:genetics / Q000821:virology; D006801:Humans; D015994:Incidence; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics / Q000821:virology; D016415:Sequence Alignment / Q000379:methods; D011198:Solanum tuberosum / Q000821:virology*; D014462:Ultrafiltration