Antibiotic resistance in lactic acid bacteria and Micrococcaceae/Staphylococcaceae isolates from artisanal raw milk cheeses, and potential implications on cheese making.
Antibiotic susceptibility against 19 antimicrobial agents was evaluated in isolates of the genera Lactococcus (46 isolates), Leuconostoc (22), Lactobacillus (19), Staphylococcus (8), Enterococcus (7), and Microccoccus/Kocuria (5) obtained from the predominant microflora of nonrecent and recent types of artisanal raw cow's milk cheeses. Beta-lactams showed broad activity against all genera, although leuconostocs and lactobacilli were highly resistant to oxacillin (80% to 95.5%). Resistance to aminoglycosides was frequent for lactococci and enterococci (particularly for streptomycin), whereas lower rates of resistance were detected for lactobacilli and leuconostocs. Technologically interesting traits for the food industry were distributed among isolates that showed different degrees of resistance to common antibiotics. However, isolates showing resistance to less than 2 antibiotics were mainly those with properties of greatest technological interest (acidifying activity, proteolytic/lipolytic activities, or diacetyl production).
Rodríguez-Alonso
P
P
Dairy Science and Technology Laboratory, Agricultural Research Center of Mabegondo (CIAM), Xunta de Galicia, 15080 A Coruña, Spain.
Fernández-Otero
C
C
Centeno
J A
JA
Garabal
J I
JI
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002611:Cheese / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods*; D019649:Food Industry; D005516:Food Microbiology; D006095:Gram-Positive Cocci / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016987:Gram-Positive Rods / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008835:Micrococcaceae / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D013030:Spain; D040141:Staphylococcaceae / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D013997:Time Factors
D000900:Anti-Bacterial Agents