Proline as a stress protectant in the yeast Saccharomyces cerevisiae: effects of trehalose and PRO1 gene expression on stress tolerance.
Proline is considered to be a stress protectant in the yeast Saccharomyces cerevisiae, as is trehalose. In this study, we constructed yeast strains that accumulate proline but not trehalose and that increase proline levels in response to stress. Our results suggest that proline does not complement the function of trehalose, and that moderate expression of the PRO1 gene encoding gamma-glutamyl kinase is important to stress tolerance of yeast cells.
Kaino
Tomohiro
T
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Takagi
Hiroshi
H
D000222:Adaptation, Physiological / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D015870:Gene Expression; D005800:Genes, Fungal; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011392:Proline / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological; D014199:Trehalose / Q000494:pharmacology*
D017931:DNA Primers; D011392:Proline; D014199:Trehalose; D017851:Phosphotransferases (Carboxyl Group Acceptor); C020650:glutamate 5-kinase