High-performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats.
A high-performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna C(18) column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5-100.0 microg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069-18.4% (RSD%), and within 20% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <15% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and to nutraceutical product analysis.
Bertram
Rebecca M
RM
Department of Pharmaceutical Sciences and Pharmacology, Washington State University, Pullman, 99164-6534, USA.
Takemoto
Jody K
JK
Remsberg
Connie M
CM
Vega-Villa
Karina R
KR
Sablani
Shyam
S
Davies
Neal M
NM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D057230:Limit of Detection; D008297:Male; D010936:Plant Extracts / Q000032:analysis* / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000032:analysis* / Q000652:urine*
D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad