Changes of mitochondria and relocation of the apoptosis-inducing factor during apoptosis.
During apoptosis, apoptosis-inducing factor (AIF) is released from the mitochondrial intermembrane space to the cytosol and to the nucleus. We analyzed AIF in HeLa cells driven to apoptosis by either etoposide or actinomycin D, and we observed changes in the structure and function of mitochondria as well as the translocation of cytochrome c and AIF from mitochondria to the nucleus in early apoptosis. In cells with fragmented chromatin (i.e., in late apoptosis), the immunolabeling for AIF appeared to be distinct from chromatin, being mainly confined to mitochondria.
Scovassi
A Ivana
AI
Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Pavia, Italy. scovassi@igm.cnr.it
Soldani
Cristiana
C
Veneroni
Paola
P
Bottone
Maria Grazia
MG
Pellicciari
Carlo
C
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D051033:Apoptosis Inducing Factor / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D013997:Time Factors
D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D051033:Apoptosis Inducing Factor; D002843:Chromatin; D018840:HSP70 Heat-Shock Proteins; D003609:Dactinomycin; D005047:Etoposide; D045304:Cytochromes c