title: A replicable tetrahedral nanostructure self-assembled from a single DNA strand.
abstract: We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Bryan
authors.initials: B
authors.lastname: Wei
authors.forename: Jeanette
authors.initials: J
authors.lastname: Nangreave
authors.forename: Chenxiang
authors.initials: C
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yongli
authors.initials: Y
authors.lastname: Mi
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009690:Nucleic Acid Conformation
chemical_list: D004277:DNA, Single-Stranded