Generation of a syngeneic mouse model to study the intraperitoneal dissemination of ovarian cancer with in vivo luciferase imaging.
In order to facilitate the discovery and investigation of anti-cancer therapeutics under physiological conditions, we have engineered the ovarian cancer cell line, HM-1/luc, in mice. This cell stably expresses firefly luciferase and produces light that can be detected using an in vivo imaging system (IVIS). Parental HM-1 cells cause severe carcinomatous peritonitis to B6C3F1 mice, but not to C57BL6 mice. Established HM-1/luc cells showed pathologically similar findings to HM-1 cells. HM-1/luc cells were injected into the peritoneal cavity of B6C3F1 mice and IVIS 2000 was conducted weekly after inoculation to monitor intra-peritoneal tumor growth. The mice were divided into three groups: non-CDDP-treated (control) and CDDP-treated (0.2 and 0.4 mg). A disease-suppressive effect of the CDDP was reflected by the significantly prolonged survival of the CDDP-treated mice (control 23 +/- 1.9 days, CDDP 0.2 mg 29.6 +/- 2.9 days; p < 0.05); the total photon and area of flux were decreased. The optical imaging of intraperitoneal tumors via in vivo bioluminescence is effective for noninvasive monitoring and semi-quantitative analysis. Our syngeneic mouse model has the relevant clinical features of ovarian cancer, which makes it a useful model for developing new ovarian cancer therapies.
Toyoshima
Masafumi
M
Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan. m-toyo@mail.tains.tohoku.ac.jp
Tanaka
Yoshinori
Y
Matumoto
Mitsuyo
M
Yamazaki
Miyuki
M
Nagase
Satoru
S
Sugamura
Kazuo
K
Yaegashi
Nobuo
N
D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D008156:Luciferases; D051379:Mice; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000175:diagnosis / Q000556:secondary*
D008156:Luciferases