Study of the photodynamic effect on the A549 cell line by atomic force microscopy and the influence of green tea extract on the production of reactive oxygen species.
We studied the morphology of the A549 cell line (human lung carcinoma cells) before and after photodynamic therapy (PDT) by atomic force microscopy. PDT was induced by an efficient light-emitting diode source with total light dose of 15 J cm(-2) in the presence of the sensitizer zinc-5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrine. In the presence of molecular oxygen, light activation of the photosensitizer, which accumulates in cancer cells, leads to the local production of reactive oxygen species (ROS). This is one of several reasons leading to cell death, and in some cases we could observe signs of apoptosis. We detected the kinetics of ROS production to be dependent on the presence of green tea extract.
Tomankova
Katerina
K
Department of Medical Biophysics, Faculty of Medicine, Palacky University in Olomouc, Olomouc, Czech Republic. katerina.tomankova@upol.cz
Kolarova
Hana
H
Bajgar
Robert
R
Jirova
Dagmar
D
Kejlova
Kristina
K
Mosinger
Jiri
J
D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008027:Light; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D008665:Metalloporphyrins / Q000494:pharmacology; D018625:Microscopy, Atomic Force / Q000379:methods*; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013662:Tea / Q000737:chemistry*
D008665:Metalloporphyrins; D017319:Photosensitizing Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013662:Tea; C474512:zinc-tetrakis(p-sulfonatophenyl)porphyrin; D010100:Oxygen