DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters.
OBJECTIVE
To investigate the expression of death decoy receptor 3 (DcR3) and survivin in colorectal carcinoma.


METHODS
Tumor and normal tissues were taken from a total of 100 colorectal carcinoma patients during surgery, and the expression of DcR3 and survivin was examined by immunohistochemistry, Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR) analyses.


RESULTS
RT-PCR showed that the expression levels of DcR3 mRNA (0.846+/-0.242, P<0.01) and survivin mRNA (0.7835+/-0.2392, P<0.01) in colorectal cancer tissues were significantly higher than those in adjacent normal tissues. Western blotting showed that the expression levels of DcR3 protein (0.795+/-0.261, P<0.01) and survivin protein (0.6765+/-0.1351, P<0.01) in tumor tissues were significantly higher than those in non-cancer tissues. The immunohistochemical streptavidin-peroxidase (SP) method showed that the positive expression rates of DcR3 and survivin were 67.0% and 58.0% in colorectal cancer tissues, and 18.0% and 3.0% in non-cancerous colorectal tissues (P<0.05), respectively. The positive correlations of DcR3 (P<0.01) and survivin (P<0.01) to the differentiation of colorectal carcinoma cells, lymph node metastasis, and pathological stage were observed. The expression of DcR3 and survivin was found to be positively correlated to clinicopathologic parameters of colorectal carcinoma.


CONCLUSIONS
The overexpressed DcR3 and survivin in colorectal cancer may contribute to the development of the cancer. The monitoring of these two proteins may be useful for the diagnosis, differentiation, metastasis, and determination of stages of colorectal carcinoma.
Liang
Qi-lian
QL
Department of Medical Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China. lianqilian@gdmc.edu.cn
Wang
Bi-rong
BR
Li
Guo-hong
GH
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D053323:Receptors, Tumor Necrosis Factor, Member 6b / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000077022:Survivin
C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D053323:Receptors, Tumor Necrosis Factor, Member 6b; D000077022:Survivin; C505911:TNFRSF6B protein, human