title: Caspase-dependent apoptosis of ON bipolar cells in the goldfish marginal retina.
abstract: Progenitor cells proliferate at the retinal margin of adult goldfish, so that the developmental stages of maturing retinal cells are arranged in temporal sequence along the periphero-central axis. We studied the caspase-dependent apoptosis of ON bipolar cells, which were labeled by an antibody to protein kinase C (PKC). In both primary and secondary growth zones of the marginal retina, PKC+ cell somata were of two distinct sizes, large and small. A caspase inhibitor (zVAD-fmk), injected into the vitreous, increased the number of both large and small PKC+ somata (times approximately 2 and 1.1, respectively) in both growth zones. These data show that about one-third of immature ON bipolar cells were removed by caspase-dependent apoptosis before being incorporated into the mature retina.
authors.affiliation: Toho University, Miyama, Funabashi, Chiba, Japan.
authors.forename: Taka-aki
authors.initials: TA
authors.lastname: Mizuno
authors.forename: Teruya
authors.initials: T
authors.lastname: Ohtsuka
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D020169:Caspases / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006054:Goldfish; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D051245:Retinal Bipolar Cells / Q000166:cytology / Q000187:drug effects*; D012162:Retinal Degeneration / Q000201:enzymology / Q000503:physiopathology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D004791:Enzyme Inhibitors; C484771:benzyloxycarbonyl-valyl-aspartic acid fluoromethyl ketone; D011493:Protein Kinase C; D020169:Caspases