Crystallization of mitochondrial rhodoquinol-fumarate reductase from the parasitic nematode Ascaris suum with the specific inhibitor flutolanil.
In adult Ascaris suum (roundworm) mitochondrial membrane-bound complex II acts as a rhodoquinol-fumarate reductase, which is the reverse reaction to that of mammalian complex II (succinate-ubiquinone reductase). The adult A. suum rhodoquinol-fumarate reductase was crystallized in the presence of octaethyleneglycol monododecyl ether and n-dodecyl-beta-D-maltopyranoside in a 3:2 weight ratio. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 123.75, b = 129.08, c = 221.12 A, and diffracted to 2.8 A resolution using synchrotron radiation. The presence of two molecules in the asymmetric unit (120 kDa x 2) gives a crystal volume per protein mass (V(M)) of 3.6 A(3) Da(-1).
Osanai
Arihiro
A
Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Harada
Shigeharu
S
Sakamoto
Kimitoshi
K
Shimizu
Hironari
H
Inaoka
Daniel Ken
DK
Kita
Kiyoshi
K
D000813:Anilides / Q000494:pharmacology*; D000818:Animals; D017165:Ascaris suum / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D010271:Parasites / Q000201:enzymology; D013379:Substrate Specificity / Q000187:drug effects; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000302:isolation & purification; D014451:Ubiquinone / Q000378:metabolism*
D000813:Anilides; D004791:Enzyme Inhibitors; D014451:Ubiquinone; C475882:flutolanil; D013385:Succinate Dehydrogenase