Preventive effect of low molecular weight glycosaminoglycan from Amussium pleuronectus (Linne) on oxidative injury and cellular abnormalities in isoproterenol-induced cardiotoxicity in Wistar rats.
The present work explores suspicious consequence of low molecular weight glycosaminoglycan (LMW-GAG) on oxidative stress and cellular abnormalities in isoproterenol (ISO)-induced myocardial infarction in an experimental model. Group-III male Wistar rats (140 +/- 10 g) were administrated by ISO (85 mg ISO/ml subcutaneously (SC) injected at the last two days of a 2-week period). Group-IV rats were treated LMW-GAG plus ISO (300 microg/day per rat SC for 1 week followed by 85 mg/kg ISO on the end last two days of the 2 - weeks). Untreated control (Group-I) and LMW-GAG drug control (Group-II) were also included. Serum and tissue lactate dehydrogenase, aminotransferases, and creatine kinase activities were increased in ISO group, which were normalized by LMW-GAG pretreatment rats. Antioxidant enzymes - superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities and non-enzymatic enzyme reduced glutathione (GSH) were decreased in the ISO induced rats, and this was increased by LMW-GAG pretreatment. Increased level of thiobarbituric acid reactive substances (TBARS) in plasma and the heart of ISO treated rats; pre s.c. injected with LMW-GAG to ISO-induced rats decreased the levels of TBARS. Histological examination revealed that the ISO-induced deleterious changes in the heart tissues were offset by LMW-GAG treatment. LMW-GAG affords considerable protection to the tissues challenged by cardiotoxicity, evidenced by its correction and restoration of serum and tissue indices of injury, to normalcy.
Saravanan
R
R
Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, 608 502 Tamil Nadu, India. saran_prp@yahoo.com
Shanmugam
A
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D007545:Isoproterenol / Q000633:toxicity*; D008297:Male; D008970:Molecular Weight; D008974:Mollusca / Q000737:chemistry*; D009203:Myocardial Infarction / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D000975:Antioxidants; D006025:Glycosaminoglycans; D007545:Isoproterenol