[Mechanisms of interaction between HIV and Mycoplasma arginini in vitro].
OBJECTIVE
Until now, the problem of effective therapy of HIV-infection is not resolved due to integrative type of interaction of HIV virus with target cell - T-lymphocyte. The study was aimed on search of method of deletion of HIV DNA-provirus from cell's genome.


METHODS
Non-pathogenic for humans Mycoplasma arginini was used for coinfection of HIV-infected cells in model systems in vitro.


RESULTS
Complex of mechanisms was documented leading to: blocking up to 50 - 60% of extracellular virus (according to titration results), cancel of apoptosis in infected cells stained on Hoechst, formation of defective vif(-) virions, which together with arginine-desaminase of M. arginini arrange permissive conditions for activation of cellular APOBEC3G with subsequent disruption of DNA- provirus and blocking of viral infection. As studies of ultrastructure showed, listed events resulted from direct interaction of HIV with mycoplasma.


CONCLUSIONS
The elimination of HIV DNA-provirus is possible by co-infection of T-lymphocytes with M. arginini.
Miller
G G
GG
Rakovskaia
I V
IV
Pokidysheva
L N
LN
Korablina
E V
EV
Titova
I V
IV
D000071480:APOBEC-3G Deaminase; D003564:Cytidine Deaminase / Q000096:biosynthesis*; D004279:DNA, Viral / Q000235:genetics / Q000502:physiology; D015894:Genome, Human; D015658:HIV Infections / Q000382:microbiology / Q000628:therapy / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D009174:Mycoplasma / Q000502:physiology*; D009504:Neutrophils / Q000821:virology; D011533:Proviruses / Q000235:genetics* / Q000502:physiology; D013601:T-Lymphocytes / Q000382:microbiology / Q000821:virology; D014779:Virus Replication
D004279:DNA, Viral; D006867:Hydrolases; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase