Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect.
Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect, a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.
Schracke
Nadine
N
Febit biomed gmbh, D-69120 Heidelberg, Germany. nadine.schracke@febit.de
Kornmeyer
Tobias
T
Kränzle
Marcel
M
Stähler
Peer F
PF
Summerer
Daniel
D
Beier
Markus
M
D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*