Rab35 controls actin bundling by recruiting fascin as an effector protein.
Actin filaments are key components of the eukaryotic cytoskeleton that provide mechanical structure and generate forces during cell shape changes, growth, and migration. Actin filaments are dynamically assembled into higher-order structures at specified locations to regulate diverse functions. The Rab family of small guanosine triphosphatases is evolutionarily conserved and mediates intracellular vesicle trafficking. We found that Rab35 regulates the assembly of actin filaments during bristle development in Drosophila and filopodia formation in cultured cells. These effects were mediated by the actin-bundling protein fascin, which directly associated with active Rab35. Targeting Rab35 to the outer mitochondrial membrane triggered actin recruitment, demonstrating a role for an intracellular trafficking protein in localized actin assembly.
Zhang
Jun
J
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Fonovic
Marko
M
Suyama
Kaye
K
Bogyo
Matthew
M
Scott
Matthew P
MP
D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004330:Drosophila / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism; D041681:NIH 3T3 Cells; D011554:Pseudopodia / Q000378:metabolism / Q000648:ultrastructure; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D002352:Carrier Proteins; D029721:Drosophila Proteins; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; C020324:fascin; C511183:RAB35 protein, human; C543586:Rab35 protein, Drosophila; C543587:Rab35 protein, mouse; D020691:rab GTP-Binding Proteins