[Antisense oligonucleotides targeting protein kinase C alpha inhibits the proliferation of A549 cells].
OBJECTIVE
To investigate the effects of antisense oligonucleotides (ASODN) targeting protein kinase C alpha (PKCalpha) on the proliferation of A549 cells.


METHODS
PKCalpha ASODN and random oligonucleotides (RODN) were transfected into A549 cells mediated by polyethyleneimine, and the proliferation and clone formation of A549 cells were detected by CCK-8 and clone formation assay, respectively. The expression of PKCalpha in the transfected cells was analyzed by RT-PCR and Western blotting.


RESULTS
Compared with those in the control group, PEI group and PEI-RODN group, the proliferation and clone formation of A549 cells treated with ASODN targeting PKCalpha were significantly inhibited (P<0.05). The expressions of PKCalpha mRNA and protein in PKCalpha ASODN-transfected A549 cells were significantly lower than those in the other 3 groups (P<0.05).


CONCLUSIONS
The PKCalpha ASODN mediated by PEI down-regutates the expression of PKCalpha gene and suppress the proliferation and clone formation of A549 cells.
Yan
Yu-Xia
YX
Department of Biochemistry, Department of Respiratory Medicine, Guangzhou, China. yanyuxia68@126.com
Jiang
Jian-Wei
JW
Huang
Zhi-Hong
ZH
Wu
Zhi-Hui
ZH
Lin
Chun-Lan
CL
Wu
Feng-Yun
FY
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D051571:Protein Kinase C-alpha / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D051571:Protein Kinase C-alpha