Two novel antimicrobial peptides from centipede venoms.
Centipede venoms are complex mixtures of biochemically and pharmacologically active components such as peptides and proteins. Very few are known about their pharmacological actions. The present work reports the structural and functional characterization of two antimicrobial peptides (scolopin 1 and -2) identified from centipede venoms of Scolopendra subspinipes mutilans by Sephadex gel filtration and reverse-phase high-performance liquid chromatography (RP-HPLC). The amino acid sequences of scolopin 1 and -2 were FLPKMSTKLRVPYRRGTKDYH and GILKKFMLHRGTKVYKMRTLSKRSH determined by Edman degradation and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Both scolopin 1 and -2 showed strong antimicrobial activities against tested microorganisms including Gram-positive/negative bacteria and fungi. They also showed moderate hemolytic activity against both human and rabbit red cells. This is the first report of antimicrobial peptides from centipedes.
Peng
Kanfu
K
Department of Kidney, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Kong
Yi
Y
Zhai
Lei
L
Wu
Xiongfei
X
Jia
Peng
P
Liu
Jingze
J
Yu
Haining
H
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents; D001180:Arthropod Venoms / Q000737:chemistry* / Q000494:pharmacology*; D001181:Arthropods / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000890:Anti-Infective Agents; D001180:Arthropod Venoms; D010455:Peptides