Rapid detection of a point mutation in the parC gene associated with decreased susceptibility to fluoroquinolones in Mycoplasma bovis.
Comparison of the quinolone resistance-determining regions (QRDRs) in 42 Mycoplasma bovis clinical isolates revealed amino acid substitutions at both GyrA (position 83) and ParC (position 84) in 10/11 enrofloxacin-resistant strains. The mutation present in the parC QRDR was discriminative for enrofloxacin resistance by parC PCR-restriction fragment length polymorphism. Comparison of molecular profiles by insertion sequence typing suggests that the currently prevalent enrofloxacin-resistant M. bovis strain evolved by selection under field conditions from one of the susceptible strains.
Lysnyansky
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I
Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, POB 12, Bet Dagan 50250, Israel. innal@moag.gov.il
Mikula
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I
Gerchman
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I
Levisohn
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S
D000890:Anti-Infective Agents / Q000494:pharmacology*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D045682:Mycoplasma bovis / Q000187:drug effects* / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D027101:DNA Topoisomerase IV