title: Fabrication of silk fibroin blended P(LLA-CL) nanofibrous scaffolds for tissue engineering.
abstract: Electrospinning using natural proteins and synthetic polymers offers an attractive technique for producing fibrous scaffolds with potential for tissue regeneration and repair. Nanofibrous scaffolds of silk fibroin (SF) and poly(L-lactic acid-co-epsilon-caprolactone) (P(LLA-CL)) blends were fabricated using 1,1,1,3,3,3-hexafluoro-2-propanol as a solvent via electrospinning. The average nanofibrous diameter increased with increasing polymer concentration and decreasing the blend ratio of SF to P(LLA-CL). Characterizations of XPS and (13)C NMR clarified the presence of SF on their surfaces and no obvious chemical bond reaction between SF with P(LLA-CL) and SF in SF/P(LLA-CL) nanofibers was present in a random coil conformation, SF conformation transformed from random coil to beta-sheet when treated with water vapor. Whereas water contact angle measurements conformed greater hydrophilicity than P(LLA-CL). Both the tensile strength and elongation at break increased with the content increasing of P(LLA-CL). Cell viability studies with pig iliac endothelial cells demonstrated that SF/P(LLA-CL) blended nanofibrous scaffolds significantly promoted cell growth in comparison with P(LLA-CL), especially when the weight ratio of SF to P(LLA-CL) was 25:75. These results suggested that SF/P(LLA-CL) blended nanofibrous scaffolds might be potential candidates for vascular tissue engineering.
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
authors.forename: Kuihua
authors.initials: K
authors.lastname: Zhang
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ikada
mesh_terms: D000818:Animals; D012831:Bombyx; D002208:Caproates / Q000138:chemical synthesis* / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004602:Elements; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005349:Fibroins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D007783:Lactones / Q000138:chemical synthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry; D016062:Porosity / Q000187:drug effects; D013499:Surface Properties / Q000187:drug effects; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D002208:Caproates; D004602:Elements; D007783:Lactones; D011091:Polyesters; C550605:poly(lactic acid-co-epsilon-caprolactone); D014867:Water; C121056:caprolactone; D005349:Fibroins