title: Assessment of CD4 T-lymphocyte reactivity by the Cylex ImmuKnow assay in patients following allogeneic hematopoietic SCT.
abstract: After allogeneic hematopoietic SCT (alloHSCT), immunosuppressed patients are susceptible to opportunistic infections, and uncontrolled function of the graft can result in GVHD. Accurate immune monitoring may help early detection and treatment of these severe complications. Between October 2005 and November 2007, a total of 170 blood samples were collected from 40 patients after alloHSCT in the Hadassah Hebrew University Medical Center and from 13 healthy controls. We utilized the Cylex ImmuKnow assay for CD4 ATP levels to compare known clinically immunocompromised vs immunocompetent patients after alloHSCT. We also compared the reconstitution of WBC count to the ImmuKnow results and clinical status. The patients' clinical course correlated with the stratification of immune response established by the ImmuKnow assay for solid organ transplantation (immunocompetent vs immunocompromised), and this often differed from their WBC count. On the basis of our observations, we conclude that the ImmuKnow assay is a simple and fast immune-monitoring technique for patients undergoing alloHSCT, with potential to predict clinical course and facilitate prompt management of post-HSCT complications. The assay should be evaluated prospectively in clinical trials.
authors.affiliation: Department of Bone Marrow Transplantation, Cancer Immunotherapy & Immunobiology Research Center, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
authors.forename: B
authors.initials: B
authors.lastname: Gesundheit
authors.forename: E
authors.initials: E
authors.lastname: Budowski
authors.forename: M
authors.initials: M
authors.lastname: Israeli
authors.forename: M Y
authors.initials: MY
authors.lastname: Shapira
authors.forename: I B
authors.initials: IB
authors.lastname: Resnick
authors.forename: R
authors.initials: R
authors.lastname: Bringer
authors.forename: Y
authors.initials: Y
authors.lastname: Azar
authors.forename: S
authors.initials: S
authors.lastname: Samuel
authors.forename: L
authors.initials: L
authors.lastname: Dray
authors.forename: A
authors.initials: A
authors.lastname: Amar
authors.forename: D
authors.initials: D
authors.lastname: Kristt
authors.forename: R
authors.initials: R
authors.lastname: Or
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D019337:Hematologic Neoplasms / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D007121:Immunocompetence; D016867:Immunocompromised Host; D007159:Immunologic Tests / Q000379:methods*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D015166:Monitoring, Immunologic / Q000379:methods; D009894:Opportunistic Infections / Q000209:etiology / Q000276:immunology / Q000517:prevention & control; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D000255:Adenosine Triphosphate