Enantioselective analysis of R- and S-propafenone in plasma by HPLC applying column switching and liquid-liquid extraction.
Two HPLC methods are described for the enantioselective analysis of R- and S-propafenone in plasma. In a column switching approach, centrifuged plasma was injected onto a silica-based strong acid cation-exchanger and the fraction containing propafenone was switched on-line onto an enantioselective Chiralcel column for separation of the enantiomers. In another approach, propafenone was extracted from plasma by liquid-liquid extraction at pH 11.4. The extracted components were transferred into aqueous medium and separated on a Chiralcel ODR. Both methods were validated and showed comparable performance. Within-day and between-day precision was better than 5% for both methods. Linear calibration functions were obtained (r(2)>0.999), and the limit of detection for each enantiomer was 0.2 microg/mL for column switching and 0.55 microg/mL for liquid-liquid extraction. The analysis methods were applied for the determination of the effect of physical exercise on the enantiomeric ratio of R- and S-propafenone in plasma of healthy volunteers. During exercise, the concentration of both enantiomers reached a maximum, followed by a significant decrease during recovery.
Lamprecht
G
G
University of Vienna, Institute of Analytical Chemistry, Währingerstrasse 38, A-1090 Vienna, Austria. guenther.lamprecht@univie.ac.at
Stoschitzky
K
K
D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D011405:Propafenone / Q000097:blood* / Q000737:chemistry*; D013237:Stereoisomerism
D011405:Propafenone