Simultaneous detection of serum immunoglobulin G antibodies to Cryptosporidium parvum by multiplex microbead immunoassay using 3 recognized specific recombinant C. parvum antigens.
Cryptosporidiosis is a significant diarrheal disease in both humans and other mammals worldwide. In the present study, we established and validated a multiplex microbead immunoassay (MIA) for surveillance of Cryptosporidium parvum infections. In the multiplex MIA, 3 specific recombinant proteins, CP23, SA35, and SA40, were used as the capture antigens simultaneously. The antibody directed against CP23 is an index of historic infection, and those against SA35 and SA40 are indices of recent infection. The multiplex MIA yielded essentially identical results with that of monoplex MIA using these 3 recombinant proteins, and the reproducibility of the multiplex MIA results was high when standardized with a calibration curve. With multiplex MIA, we detected that the pediatric population showed a higher percentage of recent infections (seropositive rates of antibodies directed against CP23, SA35, and SA40 were 6.28%, 23.19%, and 22.71%, respectively, n = 207), whereas the adult population showed a higher percentage of historic infections (seropositive rates of antibodies directed against CP23, SA35, and SA40 were 24.40%, 11.48%, and 16.75%, respectively, n = 209).
Du
Xue-Li
XL
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Xu
Jin-Mei
JM
Hou
Min
M
Yu
Rong-Bin
RB
Ge
Jian-Jun
JJ
Zhu
Hong-Sheng
HS
Wu
Hai-Wei
HW
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D007223:Infant; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D016036:Seroepidemiologic Studies
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D011994:Recombinant Proteins