Flow linear dichroism of some prototypical proteins.
Flow linear dichroism (LD) spectroscopy provides information on the orientation of molecules in solution and hence on the relative orientation of parts of molecules. Long molecules such as fibrous proteins can be aligned in Couette flow cells and characterized using LD. We have measured using Couette flow and calculated from first principles the LD of proteins representing prototypical secondary structure classes: a self-assembling fiber and tropomyosin (all-alpha-helical), FtsZ (an alphabeta protein), an amyloid fibril (beta-sheet), and collagen [poly(proline)II helices]. The combination of calculation and experiment allows elucidation of the protein orientation in the Couette flow and the orientation of chromophores within the protein fibers.
Bulheller
Benjamin M
BM
School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
Rodger
Alison
A
Hicks
Matthew R
MR
Dafforn
Timothy R
TR
Serpell
Louise C
LC
Marshall
Karen E
KE
Bromley
Elizabeth H C
EH
King
Patrick J S
PJ
Channon
Kevin J
KJ
Woolfson
Derek N
DN
Hirst
Jonathan D
JD
D008958:Models, Molecular; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013057:Spectrum Analysis
D011506:Proteins