Time-course of the retinal nerve fibre layer degeneration after complete intra-orbital optic nerve transection or crush: a comparative study.
We examined qualitatively and quantitatively in adult rat retinas the temporal degeneration of the nerve fibre layer after intra-orbital optic nerve transection (IONT) or crush (IONC). Retinal ganglion cell (RGC) axons were identified by their heavy neurofilament subunit phosphorylated isoform (pNFH) expression. Optic nerve injury induces a progressive axonal degeneration which after IONT proceeds mainly with abnormal pNFH-accumulations in RCG axons and after IONC in RGCs somas and dendrites. Importantly, this aberrant pNFH-expression pattern starts earlier and is more dramatic after IONT than after IONC, highlighting the importance that the type of injury has on the time-course of RGC degeneration.
Parrilla-Reverter
Guillermo
G
Laboratorio de Oftalmología Experimental, Facultad de Medicina, Universidad de Murcia, 30100 Murcia, Spain.
Agudo
Marta
M
Nadal-Nicolás
Francisco
F
Alarcón-Martínez
Luis
L
Jiménez-López
Manuel
M
Salinas-Navarro
Manuel
M
Sobrado-Calvo
Paloma
P
Bernal-Garro
José M
JM
Villegas-Pérez
María P
MP
Vidal-Sanz
Manuel
M
D000818:Animals; D001369:Axons / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D009409:Nerve Crush; D009412:Nerve Fibers / Q000378:metabolism / Q000473:pathology; D016900:Neurofilament Proteins / Q000378:metabolism; D020221:Optic Nerve Injuries / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012162:Retinal Degeneration / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000473:pathology*
D016900:Neurofilament Proteins