[Identification of human testicular embryonal carcinoma proteins by two-dimensional gel electrophoresis and mass spectrometry].
OBJECTIVE
To separate and identify human testicular embryonal carcinoma proteomics using two-dimensional electrophoresis (2-DE) and mass spectrometry.


METHODS
Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of the samples. After silver staining, PDQuest 7.30 image analysis software was applied to analyze the 2-DE images. Three of the proteins highly expressed in human testicular embryonal carcinoma were identified by matrix-assisted laser adsorption/ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS).


RESULTS
2-DE effectively screened the differentially expressed proteins in the carcinoma tissues. Three proteins highly expressed in the carcinoma were successfully identified.


CONCLUSIONS
The proteins of human testicular embryonal carcinoma can be effectively separated and analyzed using 2-DE and mass spectrometry. Proteomic analysis offers a new means for further study of this carcinoma.
Li
Fei
F
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zou
Ya-guang
YG
Zhou
Qi-zhao
QZ
Li
Tie-qiu
TQ
Guo
Wen-bin
WB
Jing
Xiao-wei
XW
Mao
Xiang-ming
XM
Tan
Wan-long
WL
Zheng
Shao-bin
SB
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D018236:Carcinoma, Embryonal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
D014408:Biomarkers, Tumor