title: Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides.
abstract: In this paper, an acetylcholinesterase (AChE)/dendrimers polyamidoamine (PAMAM)-Au/Carbon nanotubes (CNTs) multilayer modified electrode based on LbL self-assembled technique was employed in the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provided a favorable environment to the immobilization of AChE. The modified films also improved the electrocatalytic characteristics and electron transfer speed between the films and the surface of electrode. The PAMAM-Au nanoparticles were characterized by SEM and UV-VIS methods. A set of experimental conditions were also optimized for the detection of the pesticides. A linear response over carbofuran concentration in the range of 4.8x10(-9)M to 0.9x10(-7)M was exhibited with a detection limit of 4.0x10(-9)M. The biosensor showed high sensitivity, good stability and reproducibility with promising application.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai, PR China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Qu
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002235:Carbofuran / Q000032:analysis; D050091:Dendrimers / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D050091:Dendrimers; D037742:Nanotubes, Carbon; C104700:PAMAM Starburst; D010575:Pesticides; D006046:Gold; D000110:Acetylcholinesterase; D002235:Carbofuran