[An improved liquid nitrogen freezing method for establishing canine models of femoral head necrosis].
OBJECTIVE
To establish an canine model of femoral head osteonecrosis using an improved liquid nitrogen freezing method.


METHODS
Sixteen adult canines were divided into 4 groups at random and subjected to instant freezing of the unilateral femoral head with liquid nitrogen. The dogs were observed at 2, 4, 8, and 16 weeks after the operation for radiographic, gross and pathological changes of the femoral head.


RESULTS
Significant radiographic, gross and pathological changes occurred in the femoral head after the freezing.


CONCLUSIONS
Improved liquid nitrogen freezing of the femoral head provides a simple and convenient method for establishing animal models of femoral head osteonecrosis.
Liu
Guo-zhu
GZ
Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Yang
Tong-tao
TT
Han
Jian-wei
JW
Wu
Jin
J
Wen
Yan-hua
YH
Gao
Jie
J
Ma
Bao-an
BA
D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005271:Femur Head Necrosis; D005615:Freezing; D008297:Male; D009584:Nitrogen / Q000737:chemistry*
D009584:Nitrogen