title: [ARTCEREB irrigation and perfusion solution for cerebrospinal surgery: pharmacological assessment using human astrocytes exposed to test solutions].
abstract: ARTCEREB irrigation and perfusion solution (Artcereb) is a preparation intended for the irrigation and perfusion of the cerebral ventricles, and it is therefore important to evaluate the effects of Artcereb on brain cells. In vitro assessment of the effects of Artcereb in cell cultures of human fetal astrocytes was conducted in comparison with normal saline and lactated Ringer's solution. The effects of exposure to Artcereb were evaluated based on microscopic images of the mitochondria stained with rhodamine 123. The effects of exposure to Artcereb on cell function were also evaluated by quantitative analysis of mitochondrial activity based on rhodamine 123 and (3)H-thymidine incorporation. Morphological changes in nuclear structure were also evaluated. The results of the present study showed that cell function in cell cultures of human astrocytes was relatively unaffected by exposure to Artcereb as compared with normal saline or lactated Ringer's solution, suggesting that Artcereb has less effect on brain cells than normal saline or lactated Ringer's solution when used for the irrigation or perfusion of the cerebral ventricles.
authors.affiliation: Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan. nisimums@otsukakj.co.jp
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Nishimura
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Doi
authors.forename: Riyo
authors.initials: R
authors.lastname: Enomoto
authors.forename: Eibai
authors.initials: E
authors.lastname: Lee
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Naito
authors.forename: Aiko
authors.initials: A
authors.lastname: Yamauchi
mesh_terms: D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D002552:Cerebral Ventricles; D006801:Humans; D008928:Mitochondria; D019635:Neurosurgical Procedures; D010477:Perfusion; D019999:Pharmaceutical Solutions / Q000009:adverse effects / Q000494:pharmacology*; D020112:Rhodamine 123; D007507:Therapeutic Irrigation; D013936:Thymidine / Q000378:metabolism
chemical_list: D019999:Pharmaceutical Solutions; D020112:Rhodamine 123; D013936:Thymidine