title: Genotypic characterization of antibiotic-resistant Salmonella enteritidis isolates in Dakar, Senegal.
abstract: BACKGROUND
  It is well established that Salmonella enterica is a major cause of food-borne disease worldwide. In Africa, according to the Who Global Salm-Surv country data bank from 2000 to 2002 Salmonella enterica serovar Enteritidis was the most common serotype involved in human salmonellosis. In Dakar this serotype of Salmonella has been reported as a frequent and an increasing cause of human infection.
  
  
  METHODS
  The genetic determinants of the antimicrobial resistance of 25 selected multiresistant strains of Salmonella enterica serovar Enteritidis referred to the National Reference Center for Enterobacteria (NRCE) in Dakar were investigated using molecular techniques.
  
  
  RESULTS
  All strains carried blaTEM 1 genes. Five harboured three types of class 1 integrons with gene cassettes dfrA15, dfrA1-aadA1 and dfrA7. Multiresistance was due to a 23 Kb conjugative plasmid. DNA fingerprinting by macrorestriction of genomic DNA revealed a single related group suggesting that strains might be clonal.
  
  
  CONCLUSIONS
  The spread of resistance genes through plasmid transfer plays an important role in the dissemination of antibiotic resistance in enteric pathogens such as Salmonella Enteritidis; the risk of transmissibility of antibiotic resistance between different bacterial strains highlights the urgent need to develop strategies to limit the spread of antimicrobial resistance among bacterial enteropathogens.
authors.affiliation: Laboratoire de Bactériologie Expérimentale, Institut Pasteur, Dakar, Sénégal. gassama@pasteur.sn
authors.forename: Amy Gassama
authors.initials: AG
authors.lastname: Sow
authors.forename: Abdoul Aziz
authors.initials: AA
authors.lastname: Wane
authors.forename: Mamadou Hadi
authors.initials: MH
authors.lastname: Diallo
authors.forename: Cheikh Saad-Bouh
authors.initials: CS
authors.lastname: Boye
authors.forename: Awa
authors.initials: A
authors.lastname: Aïdara-Kane
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D032023:Integrons; D010957:Plasmids; D015995:Prevalence; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000201:enzymology / Q000235:genetics*; D012675:Senegal / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001618:beta-Lactamases; C034413:beta-lactamase TEM-1