High-throughput SPR biosensor.
Surface plasmon resonance (SPR) imaging technique is label free, real-time, and high-throughput analysis method for interaction studies with array format. The application of SPR imaging for the small molecule arrays, which were fabricated by photoaffinity crosslinking, can be the first screening step for reverse chemical genomics. The fabrication process of sugar array and sugar-lectin interaction study was demonstrated. The protocol of array fabrication did not require any chemical modifications of sugar chains for immobilizations. The biotinylated sugars were used to investigate signal ratios between lectin and antistreptavidin antibody binding. And it seemed that signal normalization could be achieved, even though the accurate densities of immobilized sugars were unclear.
Kyo
Motoki
M
Biotechnology Development Department, Toyobo Co., Ltd., Osaka, Japan.
Ohtsuka
Kimihiko
K
Okamoto
Eiji
E
Inamori
Kazuki
K
D000345:Affinity Labels; D001710:Biotin; D002241:Carbohydrates; D057166:High-Throughput Screening Assays / Q000379:methods; D037102:Lectins; D008967:Molecular Biology / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods*
D000345:Affinity Labels; D002241:Carbohydrates; D037102:Lectins; D001710:Biotin