Preparation and epitope mapping of a monoclonal antibody against Tri a Bd 27K, a major wheat allergen.
We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence (154)VPWVVVDGKPL(164) of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.
Komiyama
Nobuko
N
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
Hiemori
Miki
M
Kimoto
Masumi
M
Suzuki
Makiko
M
Yamashita
Hiromi
H
Takahashi
Yoshitaka
Y
Tsuji
Hideaki
H
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D018604:Epitope Mapping; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D017931:DNA Primers; C539494:Tri a Bd 27K allergen, Triticum aestivum