Semiochemicals used in host location by the coffee berry borer, Hypothenemus hampei.
The coffee berry borer, Hypothenemus hampei is a serious pest in many coffee growing countries. Electrophysiological and behavioral responses of H. hampei to volatiles of different phenological stages of coffee, Coffea arabica, fruits were studied in order to identify volatile semiochemicals used in host location. Volatiles were collected from different phenological stages of C. arabica fruit by air entrainment. Electrophysiological recordings were made from insect antennae. Behavioral assays were carried out using a Perspex four-arm olfactometer. Insects spent significantly more time in the region of the olfactometer where ripe and dry fruit volatiles were present compared to control regions. Coupled gas chromatography--electroantennography revealed the presence of six electrophysiologically active compounds in C. arabica volatiles. These were identified by using GC and GC-MS as methylcyclohexane, ethylbenzene, nonane, 1-octen-3-ol, (R)-limonene, and (R)-3-ethyl-4-methylpentanol. In the olfactometer bioassay, H. hampei showed a significant response to 3-ethyl-4-methylpentanol, methylcyclohexane, nonane, ethylbenzene, and a synthetic blend of these four compounds. Attraction to the synthetic blend was comparable to that for the natural sample. The significance of the study is discussed in terms of semiochemical based pest management methods of the coffee berry borer.
Mendesil
Esayas
E
Department of Biological Chemistry, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK.
Bruce
Toby J A
TJ
Woodcock
Christine M
CM
Caulfield
John C
JC
Seyoum
Emiru
E
Pickett
John A
JA
D000383:Agriculture; D000818:Animals; D001522:Behavior, Animal; D040503:Coffea / Q000737:chemistry*; D005260:Female; D005638:Fruit / Q000737:chemistry / Q000254:growth & development; D007300:Insect Control; D010675:Pheromones / Q000737:chemistry* / Q000302:isolation & purification; D014835:Volatilization; D048889:Weevils / Q000502:physiology*
D010675:Pheromones