title: Sperm meiotic segregation and aneuploidy in a 46,X,inv(Y),t(10;15) carrier: case report.
abstract: OBJECTIVE
  To analyze the meiotic segregation and an interchromosomal effect in sperm of an inv(Y) (p11.1;q11.2),t(10;15) (q25.2;q12) carrier.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Research institute.
  
  
  METHODS
  Man with a karyotype 46,X,inv(Y),t(10;15), normal sperm parameters, and secondary infertility.
  
  
  METHODS
  Multicolor fluorescence in situ hybridization using probes for chromosomes 10, 15, 8, 18, 21, X, and Y.
  
  
  METHODS
  Frequencies of meiotic segregation products and aneuploidy of chromosomes 8, 18, 21, X, and Y.
  
  
  RESULTS
  The most frequent type of meiotic segregation was the alternate (40.82%), followed by the adjacent 1 (28.09%), adjacent 2 (16.33%), and 3:1 (9.91%) segregations. Neither deviation from the expected 1:1 ratio of the X- and Y-bearing spermatozoa nor any evidence of an interchromosomal effect on aneuploidy of chromosomes X, Y, 8, 18, and 21 and diploidy was observed in the carrier compared with control donors. The disomies of chromosomes 8 and 21 were equally frequent in X- and Y-bearing spermatozoa of the carrier.
  
  
  CONCLUSIONS
  The fluorescence in situ hybridization analysis of meiotic segregation and aneuploidy helps to personalize the reproductive risk in carriers of balanced structural chromosomal aberrations. Complete information concerning the quality of spermatogenesis is necessary in all donors (both translocation carriers and controls) implicated in interchromosomal effect studies.
authors.affiliation: Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic. vozdova@vri.cz
authors.forename: Miluse
authors.initials: M
authors.lastname: Vozdova
authors.forename: Eva
authors.initials: E
authors.lastname: Oracova
authors.forename: Renata
authors.initials: R
authors.lastname: Gaillyova
authors.forename: Jiri
authors.initials: J
authors.lastname: Rubes
mesh_terms: D000328:Adult; D000782:Aneuploidy; D016022:Case-Control Studies; D007446:Chromosome Inversion; D020090:Chromosome Segregation / Q000235:genetics / Q000502:physiology; D002879:Chromosomes, Human, Pair 10; D002884:Chromosomes, Human, Pair 15; D041322:Chromosomes, Human, Y / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000235:genetics*; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000002:abnormalities / Q000378:metabolism*; D014178:Translocation, Genetic; D055815:Young Adult