title: Patterned hybrid nanohole array surfaces for cell adhesion and migration.
abstract: We report the fabrication of hybrid nanohole array surfaces to study the role of the surface nanoevironment on cell adhesion and cell migration. We use polystyrene beads and reactive ion etching to control the size and the spacing between nanoholes on a tailored self-assembled monolayer inert gold surface. The arrays were characterized by scanning electron microscopy and brightfield microscopy. For cell adhesion studies, cells were seeded to these substrates to study the effect of ligand spacing on cell spreading, stress fiber formation, and focal adhesion structure and size. Finally, comparative cell migration rates were examined on the various nanohole array surfaces using time-lapse microscopy.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Westcott
authors.forename: Yi
authors.initials: Y
authors.lastname: Lou
authors.forename: John F
authors.initials: JF
authors.lastname: Muth
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Yousaf
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D005347:Fibroblasts / Q000166:cytology; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008024:Ligands; D051379:Mice; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D022502:Stress Fibers / Q000378:metabolism; D013499:Surface Properties; D013997:Time Factors
chemical_list: D008024:Ligands; D006046:Gold