Distribution of Malassezia species in patients with pityriasis versicolor in Northern Iran.
OBJECTIVE
Malassezia yeasts are globally distributed agents of pityriasis versicolor and are implicated in the pathogenesis of seborrhoeic and atopic dermatitis. The aim of this study is to identify the Malassezia species obtained from pityriasis versicolor patients, using morphological, biochemical, physiological as well as Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods.


METHODS
The identification of Malassezia species is performed according to microscopic features and physiological characteristics, including catalase reaction and Tween assimilation tests. The DNA is extracted from cultured Malassezia using the glass bead, phenol-chloroform method. The internal transcribed spacer 1(ITS1) region is amplified and there is restricted digestion of the PCR products with two enzymes Cfo I and Bst F5I.


RESULTS
The most commonly isolated species is M. globosa (47.6%). RFLP analysis of the PCR products of the ITS1 region is in complete agreement with those from the DNA sequences of the internal transcribed spacer (ITS) 1 region and the biochemical tests.


CONCLUSIONS
Based on the findings of this study, it can be concluded that PCR-RFLP is a relatively simple and quick method, completely comparable to the routine methods used for Malassezia identification.
Shokohi
T
T
Department of Medical Mycology and Parasitology, Sari Medical School, Sari, Iran. shokohi.tahereh@gmail.com
Afshar
P
P
Barzgar
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007492:Iran; D008290:Malassezia / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D016533:Mycological Typing Techniques; D012150:Polymorphism, Restriction Fragment Length; D011136:Polysorbates / Q000378:metabolism; D021521:Ribotyping; D014010:Tinea Versicolor / Q000382:microbiology*; D055815:Young Adult
D004271:DNA, Fungal; D011136:Polysorbates; D002374:Catalase