title: [Expression of plasma thrombospondin-1 in renal interstitial fibrosis].
abstract: OBJECTIVE
  To observe the expression of plasma thrombospondin-1(TSP-1) at different time in protein-overload rats and to analyze the relationship between plasma TSP-1 expression and renal interstitial fibrosis.
  
  
  METHODS
  Forty-five male Sprague-Dawley rats were randomly divided into a bovine serum albumin (BSA) group and a control group after uninephrectomization. Rats with protein overload nephropathy induced by intraperitoneally injected BSA were used as a model (control group received saline). At the 1st, 5th, and 9th weekend, the level of 24 h proteinuria and renal function was assessed. Pathological changes were observed by electron and fluorescent microscopy. The expression of plasma TSP-1 was detected by Western blot. The relationship between plasma TSP-1 and tubulointerstitial lesions (TIL) score was analyzed.
  
  
  RESULTS
  Twenty-four hour proteinuria and blood urea nitrogen (BUN) significantly increased in protein-overload rats compared with those in the control group. While protein-overload rats developed more severe fibrosis in the tubular and interstitium. Glomerulosclerosis index and TIL score were upregulated compared with those in the control group. The expression of TSP-1 increased significantly at the 5th and 9th weekend. The expression of TSP-1 was positively correlated with TIL score (r=0.836, P<0.01).
  
  
  CONCLUSIONS
  Plasma TSP-1 expression is positively correlated with renal interstitial fibrosis in protein-overload rats. Plasma TSP-1 may be used for an important biomarker of renal interstitial fibrosis.
authors.affiliation: Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Yuncheng
authors.initials: Y
authors.lastname: Xia
authors.forename: Jing
authors.initials: J
authors.lastname: Nie
authors.forename: Zhilan
authors.initials: Z
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Yinhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ling
authors.forename: Youmin
authors.initials: Y
authors.lastname: Peng
authors.forename: Fuyou
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D005355:Fibrosis / Q000378:metabolism / Q000473:pathology; D005923:Glomerulosclerosis, Focal Segmental / Q000473:pathology*; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009392:Nephrectomy; D009395:Nephritis, Interstitial / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019700:Thrombospondin 1 / Q000097:blood*
chemical_list: D019700:Thrombospondin 1