Suberin of potato (Solanum tuberosum var. Nikola): comparison of the effect of cutinase CcCut1 with chemical depolymerization.
Chemical and enzymatic depolymerizations of suberin isolated from potato peel ( Solanum tuberosum var. Nikola) were performed under various conditions. Enzymatic hydrolysis with cutinase CcCut1 and chemical methanolysis with NaOMe of suberin yielded monomeric fragments, which were identified as TMS derivatives with GC-MS and GC-FID. The solid, hydrolysis-resistant residues were analyzed with solid state (13)C CPMAS NMR, FT-IR, and microscopic methods. Methanolysis released more CHCl(3)-soluble material than the cutinase treatment when determined gravimetrically. Interestingly, cutinase-catalyzed hydrolysis produced higher proportions of aliphatic monomers than hydrolysis with the NaOMe procedure when analyzed by GC in the form of TMS derivatives. Monomers released by the two methods were mainly alpha,omega-dioic acids and omega-hydroxy acids, but the ratios of the detected monomers were different, at 40.0 and 32.7% for methanolysis and 64.6 and 8.2% for cutinase, respectively. Thus, cutinase CcCut1 showed higher activity toward ester bonds of alpha,omega-dioic acids than toward the bonds of omega-hydroxy acids. The most abundant monomeric compounds were octadec-9-ene-1,18-dioic acid and 18-hydroxyoctadec-9-enoic acid, which accounted for ca. 37 and 28% of all monomers, respectively. The results of the analyses of the chemical and enzymatic hydrolysis products were supported by the spectroscopic analyses with FT-IR and CPMAS (13)C NMR together with the analysis of the microstructures of the hydrolysis residues by light and confocal microscopy.
Järvinen
Riikka
R
Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland. riikka.jarvinen@utu.fi
Silvestre
Armando J D
AJ
Holopainen
Ulla
U
Kaimainen
Mika
M
Nyyssölä
Antti
A
Gil
Ana M
AM
Pascoal Neto
Carlos
C
Lehtinen
Pekka
P
Buchert
Johanna
J
Kallio
Heikki
H
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D035281:Plant Tubers / Q000737:chemistry; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity
D005227:Fatty Acids; D008055:Lipids; D008563:Membrane Lipids; D011108:Polymers; C065875:suberin; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D000432:Methanol