title: Interaction of ZFPIP with PBX1 is crucial for proper expression of neural genetic markers during Xenopus development.
abstract: ZFPIP/Zfp462 has been recently identified as a new vertebrate zinc finger encoding gene whose product interacts with Pbx1. Previous work indicates that ZFPIP is maternally expressed in Xenopus laevis oocytes and plays a key role during the cleavage phase of embryogenesis. This early expression is followed by a zygotic expression which overlaps with the neural Pbx1 expression pattern, suggesting an interaction between these two partners during Xenopus neurogenesis. In order to test the physiological interaction between ZFPIP and Pbx1, we carried out a dominant negative assay in which the Pbx1 interacting domain of ZFPIP (ZFPIPp) was overexpressed in Xenopus laevis embryos. We observed that ZFPIPp ectopic expression led to abnormal en2 and N-cam expression patterns, whereas krox-20 expression was not affected. Furthermore, we showed that while ZFPIPp alone was localized in the nucleus of Cos-7 cells, additional expression of Pbx1 induced a location of ZFPIPp at the perinuclear region of the cells. These overall data suggest that ZFPIP and Pbx1 could be partners and cooperate in the regulation of essential neural genes during Xenopus development.
authors.affiliation: Institut de Génétique et Développement, UMR CNRS 6061, IFR 140, 35043 Rennes, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Laurent
authors.forename: Julie
authors.initials: J
authors.lastname: Masse
authors.forename: Stéphane
authors.initials: S
authors.lastname: Deschamps
authors.forename: Agnes
authors.initials: A
authors.lastname: Burel
authors.forename: Francis
authors.initials: F
authors.lastname: Omilli
authors.forename: Laurent
authors.initials: L
authors.lastname: Richard-Parpaillon
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pellerin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; C456283:PBX1 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins; C545512:ZNF462 protein, Xenopus