An experimental approach to study the biosynthesis of brominated metabolites by the red algal genus Laurencia.
The production of labeled brominated metabolites with radioactive (82)Br in Laurencia species was investigated as part of a study of the biosynthesis of halogenated metabolites from species belonging to the red algal genus Laurencia (Rhodomelaceae, Ceramiales). Radiobromide [(82)Br], thin-layer chromatography (TLC), and TLC-autoradioluminography (ARLG) were used. When cultured in artificial seawater medium (ASP(12)NTA including Na(82)Br) under 16:8 h light:dark (LD) illumination cycles for 24 h, each of the strains of Laurencia, Laurencia japonensis Abe et Masuda, Laurencia nipponica Yamada (laurencin-producing race and laureatin-producing race), and Laurencia okamurae Yamada, produced species- (or race-) specific (82)Br-containing metabolites. In the case of the laurencin-producing race of L. nipponica, laurencin and deacetyllaurencin were found to be produced in approximately 1:1 ratio, though laurencin is the major metabolite in the wild sample. Furthermore, when cultured in the dark, the production rates of brominated metabolites in Laurencia spp. were found to be diminished. The present study strongly indicates that the use of radiobromine [(82)Br] in combination with the TLC-ARLG method is an effective approach for investigating the biosynthesis of brominated metabolites in Laurencia.
Suzuki
Minoru
M
Division of Material Science, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan.
Takahashi
Yoshinori
Y
Nakano
Satoru
S
Abe
Tsuyoshi
T
Masuda
Michio
M
Ohnishi
Toshiyuki
T
Noya
Yoichi
Y
Seki
Koh-ichi
K
D001345:Autoradiography; D001967:Bromine Radioisotopes / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003624:Darkness; D006842:Hydrocarbons, Brominated / Q000378:metabolism*; D007553:Isotope Labeling; D044722:Laurencia / Q000378:metabolism*; D035964:Oxocins / Q000378:metabolism*
D001967:Bromine Radioisotopes; D006842:Hydrocarbons, Brominated; D035964:Oxocins; C017460:laurencin