title: A novel chemiluminescence reaction system for the determination of norfloxacin with Ag(III) complex.
abstract: A novel chemiluminescence (CL) system for the determination of norfloxacin (NFLX) is developed based on the direct CL reaction of [Ag(HIO(6))(2)](5-)-H(2)SO(4)-NFLX system. The possible mechanism of CL emission and enhancing effect was discussed by comparing UV, fluorescence and CL spectra. [Ag(HIO(6))(2)](5-) in the presence of H(2)SO(4) could produce CL emission at 490 nm, this might be caused by the excited state (O(2))(2)*. The enhancing effect of NFLX may be produced through an intermolecular energy transfer from part of (O(2))(2)* to NFLX molecule and complex of Ag(3+) and NFLX. The CL intensity emission intensity was linear in the range 1.34 x 10(-8) to 5.44 x 10(-6) gmL(-1) with correlation coefficient of 0.9982. The detection limit (s/n=3) was 3.10 x 10(-9) gmL(-1). The recovery was in the range of 90.0-104% with the RSD of 1.1-2.8%. The proposed flow injection CL method was applied satisfactorily for the determination of NFLX in capsule, human serum and urine.
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
authors.forename: Hanwen
authors.initials: H
authors.lastname: Sun
authors.forename: Peiyun
authors.initials: P
authors.lastname: Chen
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood* / Q000652:urine*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D009643:Norfloxacin / Q000097:blood* / Q000652:urine*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D009643:Norfloxacin