title: Glucose enhances collectrin protein expression in insulin-producing MIN6 beta cells.
abstract: Collectrin is a novel target gene of hepatocyte nuclear factor-1alpha in pancreatic beta-cells and controls insulin exocytosis. Although glucose is known to stimulate the expression of genes of the insulin secretory pathway, there is no information on how glucose regulates collectrin expression. We investigated the effects of glucose on the expression of collectrin in MIN6 beta-cell line. Glucose, in a dose-dependent manner, increased collectrin protein levels without changing collectrin mRNA levels and protein stability, indicating that glucose stimulation of collectrin protein expression is primarily mediated at a translational level. Although mannose and pyruvate also increased collectrin protein expression level, neither 2-deoxyglucose, mitochondrial fuels leucine and glutamate, sulphonylurea nor Ca(2+) channel blockers, mimicked the effects of glucose. These data indicate the involvement of mitochondrial TCA cycle intermediates, distal to pyruvate, in the regulation of collectrin protein expression in beta-cells.
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Saisho
authors.forename: Atsunori
authors.initials: A
authors.lastname: Fukuhara
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yasuda
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Sato
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukui
authors.forename: Hiromi
authors.initials: H
authors.lastname: Iwahashi
authors.forename: Akihisa
authors.initials: A
authors.lastname: Imagawa
authors.forename: Mitsutoki
authors.initials: M
authors.lastname: Hatta
authors.forename: Iichiro
authors.initials: I
authors.lastname: Shimomura
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamagata
mesh_terms: D000818:Animals; D002460:Cell Line; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D014176:Protein Biosynthesis / Q000187:drug effects
chemical_list: D007328:Insulin; D008562:Membrane Glycoproteins; C504407:Tmem27 protein, mouse; D005947:Glucose