title: Serine protease inhibitor cvSI-1 potential role in the eastern oyster host defense against the protozoan parasite Perkinsus marinus.
abstract: The serine protease inhibitor cvSI-1, purified from plasma of eastern oysters, inhibited the proliferation of the protozoan parasite Perkinsus marinus in vitro. In situ hybridization located cvSI-1 gene expression in basophil cells of the digestive tubules and cvSI-1 expression measured by real-time quantitative reverse transcriptase polymerase chain reaction was several hundred folds greater in digestive glands than in other organs examined or circulating hemocytes. cvSI-1 gene expression was also significantly greater in winter than in summer. Finally, cvSI-1 gene expression and plasma protease inhibitory activity in oysters selected for increased resistance to P. marinus were significantly greater than in unselected oysters. These findings support the hypothesis that cvSI-1 plays a role in eastern oyster host defense against P. marinus possibly through inhibition of parasite proliferation.
authors.affiliation: Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA. jlapeyre@agctr.lsu.edu
authors.forename: Jerome F
authors.initials: JF
authors.lastname: La Peyre
authors.forename: Qing-Gang
authors.initials: QG
authors.lastname: Xue
authors.forename: Naoki
authors.initials: N
authors.lastname: Itoh
authors.forename: Yanli
authors.initials: Y
authors.lastname: Li
authors.forename: Richard K
authors.initials: RK
authors.lastname: Cooper
mesh_terms: D056893:Alveolata / Q000502:physiology*; D000818:Animals; D001491:Basophils / Q000276:immunology; D049896:Crassostrea / Q000201:enzymology / Q000469:parasitology*; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015842:Serine Proteinase Inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors