[Oxidative stress markers in atherogenesis induced by hyperfibrinogenemia].
BACKGROUND
We studied plasmatic TNF-alpha, nitric oxide (NO) and citrulline behaviors and probable morphological mitochondrial alterations in aortic smooth muscle cells, in rats with atherogenesis induced by hyperfibrinogenemia in: A) control, B) multiple injured for 30 days and C) multiple injured for 60 days.


METHODS
Hyperfibrinogenemia induction: adrenaline injection (0,1 mg/rat/day). TNF-alpha (pg/dL) was determined by Elisa and NO (microM) and citrulline (mM) by spectrophotometry. Morphological mitochondrial alterations were studied by electronic microscopy. Variables were analized: ANOVA, r coefficient and chi2 test.


RESULTS
We observed a significant increment of TNF-alpha in multiple injured for 30 days (B) (50.05 +/- 2.29) as well as in multiple injured for 60 days (C) (74.99 +/- 2.82) related to control (A) (33.01 +/- 1.49) (p<0.001 in both groups). Citrulline presented a significant increased in (B) (5.56 +/- 0.20) and (C) (6.84 +/- 0.13) when compared to (A) (4.41 +/- 0.23) (p<0.001 in both situations). Mean while NO biodisponibility diminished significantly in (B) (8.97 +/- 0.70) and in (C) (5.32 +/- 0.68) when compared to (A) (21.65 +/- 1.74) (p<0.001 in both situations).


CONCLUSIONS
Hyperfibrinogenemia could modify the NO physiopathological pathway and produced morphological mitochondrial alterations in aortic smooth muscle cells, probably producing ischemic lesions in the vascular wall and altering the vasodilatation response.
Báez
María C
MC
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional Córdoba, Argentina.
Táran
Mariana D
MD
Campana
Vilma
V
Simes
Juan C
JC
Pons
Patricia
P
Atilio-Palma
José
J
Moya
Mónica
M
D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000209:etiology* / Q000473:pathology; D015415:Biomarkers / Q000097:blood; D002956:Citrulline / Q000097:blood*; D004285:Dogs; D005340:Fibrinogen / Q000032:analysis*; D008659:Metabolic Diseases / Q000097:blood*; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha; D002956:Citrulline; D009569:Nitric Oxide; D005340:Fibrinogen