Simultaneous determination of five benzimidazoles in feeds using high-performance capillary electrophoresis.
A high-performance capillary electrophoretic method for determination of five benzimidazoles (thiabendazole, albendazole, fenbendazole, mebendazole, oxfendazole) in animal feeds was developed. Separation was carried out in an 80.5 cm x 75 microm id fused-silica uncoated capillary and injected by hydrodynamic injection (35 mbar x 8 s) at a wavelength detection of 295 nm. The drugs were completely resolved in 50 mmol/L citric acid buffer (pH 1.9) with 30 kV at a temperature of 25 degrees C. Linear range was 1-80 microg/mL; limits of detection for the analytes were less than 2 mg/kg; the overall recoveries for benzimidazoles were >73%, with coefficients of variation below 10%.
Shen
Jianzhong
J
China Agricultural University, National Reference Laboratories for Veterinary Drug Residue, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Beijing 100193, People's Republic of China.
Tong
Jing
J
Jiang
Haiyang
H
Rao
Qinxiong
Q
Li
Na
N
Guo
Liming
L
Ding
Shuangyang
S
D000821:Animal Feed / Q000032:analysis*; D001562:Benzimidazoles / Q000032:analysis*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations / Q000032:analysis; D012015:Reference Standards
D001562:Benzimidazoles; D002021:Buffers; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations