title: In vitro anti-neoplastic activity of the ethno-pharmaceutical plant Hypericum adenotrichum Spach endemic to Western Turkey.
abstract: Hypericum perforatum (St. John's wort) is well-established for its antidepressant activity throughout the world and also various other species within this genus are used in different folk medicines. Hyperforin of St. John's wort inhibited growth of cancer cell lines and the use of hypericin (another compound of H. perforatum) in cancer photodynamic therapy is proposed. Therefore, we investigated the anti-cancer properties of H. adenotrichum Spach (Guttiferae), an endemic species in Turkey called 'kantaron', which is used for wound healing and antiseptic effects. Freeze-dried plant was extracted with petroleum ether, dichloromethane, ethyl acetate, and methanol and the bioactivity of these extracts was analysed by proliferation assay, cell death determination, by investigating protein expression profiles specific for cell cycle arrest and apoptosis as well as composition by HPLC. The strongest anti-proliferative activity was determined for the petroleum ether extract with an IpC50 of approximately 5.8 microg/ml medium (referring to 1 mg dried plant) which correlated with cyclin D1 suppression and p21 induction. This extract also induced phosphorylation of H2AX, and activated caspase-3 followed by signature-type cleavage of PARP resulting in approximately 50% apoptosis at 23.2 microg/ml after 24 h of treatment. Neither hyperforin, hypericin, or amentoflavone contributed to these properties. To the best of our knowledge, we report for the first time that the endemic plant H. adenotrichum Spach exhibits potent p53-independent anti-neoplastic properties due to yet unexplored Hypericum constituents.
authors.affiliation: Institute of Biology, Fen-Edebiyat Fakültesi, Adnan Menderes Universitesi, Aydin, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Ozmen
authors.forename: Sabine
authors.initials: S
authors.lastname: Bauer
authors.forename: Manuela
authors.initials: M
authors.lastname: Gridling
authors.forename: Judith
authors.initials: J
authors.lastname: Singhuber
authors.forename: Stanimira
authors.initials: S
authors.lastname: Krasteva
authors.forename: Sibylle
authors.initials: S
authors.lastname: Madlener
authors.forename: Than Phuong Nha
authors.initials: TP
authors.lastname: Vo
authors.forename: Nicole
authors.initials: N
authors.lastname: Stark
authors.forename: Philipp
authors.initials: P
authors.lastname: Saiko
authors.forename: Monika
authors.initials: M
authors.lastname: Fritzer-Szekeres
authors.forename: Thomas
authors.initials: T
authors.lastname: Szekeres
authors.forename: Tülay
authors.initials: T
authors.lastname: Askin-Celik
authors.forename: Liselotte
authors.initials: L
authors.lastname: Krenn
authors.forename: Georg
authors.initials: G
authors.lastname: Krupitza
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D020902:Hypericum / Q000737:chemistry; D066298:In Vitro Techniques; D007938:Leukemia / Q000188:drug therapy*; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D014421:Turkey
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts