Two-photon fluorescent probes of biological Zn(II) derived from 7-hydroxyquinoline.
A new fluorescent probe for monitoring Zn(2+) was synthesized based on the structure of 7-hydroxyquinoline. Compared with 8-substituted quinolines, the new probe exhibited higher selectivity for Zn(2+) over Cd(2+). Its fluorescence enhancement (14-fold) and nanomolar range sensitivity (K(d) = 0.117 nM) were favorable toward biological applications. Experiments also showed that a cell-permeable derivative of the new probe was potentially useful for two-photon imaging in living cells.
Chen
Xiao-Yun
XY
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Shi
Jing
J
Li
Yi-Ming
YM
Wang
Feng-Liang
FL
Wu
Xu
X
Guo
Qing-Xiang
QX
Liu
Lei
L
D045744:Cell Line, Tumor; D018360:Crystallography, X-Ray; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006912:Hydroxyquinolines / Q000138:chemical synthesis / Q000737:chemistry*; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D017785:Photons; D015032:Zinc / Q000737:chemistry*
D005456:Fluorescent Dyes; D006912:Hydroxyquinolines; D009942:Organometallic Compounds; D015032:Zinc