Anthocyanins from black soybean seed coats stimulate wound healing in fibroblasts and keratinocytes and prevent inflammation in endothelial cells.
Wound healing is a complex process that includes inflammation, tissue formation, and remodeling. While wound healing is accompanied by inflammatory reactions, chronic inflammation impairs acute wound healing. In this study, we investigated whether anthocyanins from black soybean seed coats could stimulate wound healing while preventing excessive inflammation. At 24h of treatment with anthocyanins, fibroblasts showed a significant increase in migration at 100 microg/mL whereas the migration of keratinocytes increased significantly at 50 and 100 microg/mL compared to control. Treatment of anthocyanins for 48 h significantly stimulated the migration of both human dermal fibroblasts and keratinocytes at 50 and 100 microg/mL concentrations. Treatment of cells with anthocyanins stimulated wound-induced VEGF production in fibroblasts and keratinocytes. However, anthocyanins inhibited ROS accumulation and VEGF production in TNF-alpha-stimulated endothelial cells. Furthermore, treatment of anthocyanins reduced, in a dose-dependent manner, the adhesion of inflammatory monocytes to endothelial cells. Anthocyanins also blocked both the translocation of nuclear factor-kappa B (NF-kappaB) p65 into the nucleus and the phosphorylation of the inhibitory factor kappaBalpha (IkappaBalpha). Thus, treatment with anthocyanins from black soybean seed coats may be a potential therapeutic strategy to promote wound healing and to prevent inflammation in a persistent inflammatory condition.
Nizamutdinova
Irina Tsoy
IT
Department of Pharmacology, School of Medicine and Institute of Health Sciences, Gyeongsang Nat'l University, Republic of Korea.
Kim
Young Min
YM
Chung
Jong Il
JI
Shin
Sung Chul
SC
Jeong
Yong-Kee
YK
Seo
Han Geuk
HG
Lee
Jae Heun
JH
Chang
Ki Churl
KC
Kim
Hye Jung
HJ
D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D054443:Cell Migration Assays; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D015603:Keratinocytes / Q000187:drug effects*; D008967:Molecular Biology; D017382:Reactive Oxygen Species; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D000872:Anthocyanins; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A