title: Multilocus sequence typing of Campylobacter jejuni from broilers.
abstract: Campylobacter jejuni isolates from a national Swedish Campylobacter monitoring in broilers were characterized by multilocus sequencing typing (MLST) in order to study the genetic diversity of this bacterial population. Isolates were initially characterized by pulsed-field gel electrophoresis (PFGE). One hundred were chosen for MLST genotyping. PFGE identified 69 distinct types compared to 44 different sequence types (STs) identified with MLST. Eighteen STs had not been described previously, while the remaining 26 STs were assigned to previously known clonal complexes. The majority of isolates were of genotypes noted in broilers and in humans in earlier studies. However, three clonal complexes, ST-206 complex, ST-677 complex and ST-1034 complex, previously associated with wild bird and environmental samples, were among the genotypes found. This study shows that most of the Swedish broiler isolates were of genotypes noted as common in broilers. However, it also highlights the potential influence of environmental sources on the broiler C. jejuni genotypes.
authors.affiliation: Section for Zoonotic Ecology and Epidemiology, University of Kalmar, SE-391 82 Kalmar, Sweden.
authors.forename: Petra
authors.initials: P
authors.lastname: Griekspoor
authors.forename: Eva Olsson
authors.initials: EO
authors.lastname: Engvall
authors.forename: Björn
authors.initials: B
authors.lastname: Olsen
authors.forename: Jonas
authors.initials: J
authors.lastname: Waldenström
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D017720:Molecular Epidemiology; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D013548:Sweden / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial