Matrine determination and pharmacokinetics in human plasma using LC/MS/MS.
A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the determination of matrine in human plasma extracted by isopropanol:ethyl acetate (v/v, 5:95). Rapid chromatographic separation was achieved in the mobile phase composition of 5-mM aqueous ammonium acetate and acetonitrile (v/v, 70:30) with a flow rate of 0.20ml/min. Detection was carried out using positive-ion electrospray tandem mass spectrometry on a Sciex API3000. The method was accurate, specific and sensitive for the analysis of matrine in human plasma in the concentration range of 5-2000ng/ml, when huperzine A was used as internal standard. The method facilitated a clinical pharmacokinetic study after oral administration of a single dose of matrine soft gelatin capsules (100, 200 and 400mg) in a three-period crossover design. Dose-related linear trends were observed for the AUC(0-t) and the C(max) of matrine. The t(1/2) and the T(max) of matrine were independent of the administered doses.
Zhang
Xiao-Lin
XL
Department of Clinical Pharmacology, Zhong Shan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
Xu
Hong-Rong
HR
Chen
Wei-Li
WL
Chu
Nan-Nan
NN
Li
Xue-Ning
XN
Liu
Gang-Yi
GY
Yu
Chen
C
D000470:Alkaloids / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D016018:Least-Squares Analysis; D011807:Quinolizines / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D012717:Sesquiterpenes; C050426:huperzine A; D000093842:Matrines