title: Primary structure and antibacterial activity of chicken bone marrow-derived beta-defensins.
abstract: Three biologically active beta-defensins were purified by chromatography from chicken bone marrow extract: avian beta-defensin 1 (AvBD1), AvBD2, and the newly isolated beta-defensin AvBD7. Mass spectrometry analyses showed that bone marrow-derived AvBD1, -2, and -7 peptides were present as mature peptides and revealed posttranslational modifications for AvBD1 and AvBD7 in comparison to their in silico-predicted amino acid sequences. Tandem mass spectrometry analysis using the nanoelectrospray-quadrupole time of flight method showed N-terminal glutaminyl cyclization of mature AvBD7 and C-terminal amidation of mature AvBD1 peptide, while posttranslational modifications were absent in bone marrow-derived mature AvBD2 peptide. Furthermore, mass spectrometry analysis performed on intact cells confirmed the presence of these three peptides in mature heterophils. In addition, the antibacterial activities of the three beta-defensins against a large panel of gram-positive and -negative bacteria were assessed. While the three defensins displayed similar antibacterial spectra of activity against gram-positive strains, AvBD1 and AvBD7 exhibited the strongest activity against gram-negative strains in comparison to AvBD2.
authors.affiliation: INRA, UR 1282 Infectiologie Animale et Santé Publique, F-37380 Nouzilly, France.
authors.forename: Chrystelle
authors.initials: C
authors.lastname: Derache
authors.forename: Valérie
authors.initials: V
authors.lastname: Labas
authors.forename: Vincent
authors.initials: V
authors.lastname: Aucagne
authors.forename: Hervé
authors.initials: H
authors.lastname: Meudal
authors.forename: Céline
authors.initials: C
authors.lastname: Landon
authors.forename: Agnès F
authors.initials: AF
authors.lastname: Delmas
authors.forename: Thierry
authors.initials: T
authors.lastname: Magallon
authors.forename: Anne-Christine
authors.initials: AC
authors.lastname: Lalmanach
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D030161:Avian Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001853:Bone Marrow / Q000737:chemistry*; D002645:Chickens; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization; D023083:beta-Defensins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D030161:Avian Proteins; D023083:beta-Defensins