title: Osteostatin-loaded bioceramics stimulate osteoblastic growth and differentiation.
abstract: Parathyroid hormone-related protein (PTHrP) is an important regulator of bone remodeling. Recent studies show that this protein can induce osteogenic features through its N- and C-terminal domains. Silica-based ordered mesoporous bioceramics with an SBA-15 structure - known to be bioactive and biocompatible - have recently been evaluated for their capacity to uptake and deliver L-tryptophan. This amino acid corresponds to the end position of the 107-111 domain (called osteostatin) of the native C-terminal PTHrP (107-139) fragment, whose true action in bone metabolism is still ill-defined. In the present study, we assessed some effects of the aforementioned biomaterials pressed into disks, loaded or not with osteostatin, in osteoblastic cell cultures. Our data demonstrate that both unmodified and organically modified SBA-15 loaded with this peptide increase cell growth and the expression of several osteoblastic products (alkaline phosphatase, osteocalcin, collagen, osteoprotegerin, receptor activator of nuclear factor-kappaB ligand and vascular endothelial growth factor) in osteoblastic cells. These findings support the notion that osteostatin coating confers osteogenic features to silica-based ordered mesoporous materials, which make them suitable biomaterials for bone repair.
authors.affiliation: Laboratorio de Metabolismo Mineral y Oseo, Fundación Jiménez Díaz (Capio Group), Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lozano
authors.forename: Miguel
authors.initials: M
authors.lastname: Manzano
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Doadrio
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Salinas
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez-Barrena
authors.forename: Pedro
authors.initials: P
authors.lastname: Esbrit
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003460:Crystallization / Q000379:methods; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D044162:Parathyroid Hormone-Related Protein / Q000008:administration & dosage* / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D044162:Parathyroid Hormone-Related Protein