Correlation of glioma proliferation assessed by flow cytometry with (99m)Tc-Tetrofosmin SPECT uptake.
OBJECTIVE
Brain single-photon emission computed tomography (SPECT) has been proposed as a potentially useful modality for the metabolic assessment of various brain tumors.


METHODS
In a 10-patient prospective pilot study we evaluated whether (99m)Tc-Tetrofosmin ((99m)Tc-TF) uptake correlates with glioma proliferative activity assessed by flow cytometric analysis. (99m)Tc-TF brain SPECT was performed shortly before surgical tumor excision.


RESULTS
Eight patients were diagnosed with glioblastoma multiform and 2 with anaplastic astrocytoma. All tumors were aneuploid. We found a significant positive linear correlation between (99m)Tc-TF uptake and percentage of tumor cells on the S-phase of the cell cycle (r=0.92, P=0.001).


CONCLUSIONS
Initial evidence suggests that (99m)Tc-TF could provide a non-invasive indicator of glioma proliferative activity.
Alexiou
George A
GA
Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece. alexiougrg@yahoo.gr
Tsiouris
Spyridon
S
Vartholomatos
George
G
Fotakopoulos
George
G
Papadopoulos
Athanasios
A
Kyritsis
Athanasios P
AP
Voulgaris
Spyridon
S
Fotopoulos
Andreas D
AD
D000368:Aged; D000782:Aneuploidy; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics / Q000473:pathology*; D049109:Cell Proliferation; D004247:DNA / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D015902:Gamma Cameras; D005909:Glioblastoma / Q000000981:diagnostic imaging / Q000473:pathology; D005910:Glioma / Q000000981:diagnostic imaging* / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D010865:Pilot Projects; D011446:Prospective Studies; D019275:Radiopharmaceuticals; D015899:Tomography, Emission-Computed, Single-Photon
D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C078700:technetium tc-99m tetrofosmin; D004247:DNA