title: Interferon-gamma 874A>T genetic polymorphism is associated with infectious complications following surgery in patients with thoracic esophageal cancer.
abstract: BACKGROUND
  Cytokines play a major role in the organization of orchestrated responses to infections, and there is an emerging consensus that cytokine gene polymorphisms mediate individual variations in cytokine expression. Our aim in this study was to assess whether cytokine polymorphisms were associated with infectious complications following esophagectomy in a Japanese population.
  
  
  METHODS
  The study participants were Japanese patients treated with transthoracic esophagectomy without neoadjuvant treatment. DNA was extracted from blood samples, and genetic polymorphisms for interferon (INF)-gamma, tumor necrosis factor-alpha and -beta, transforming growth factor-beta1, interleukin (IL)-1beta, IL-1 receptor antagonist, IL-2, IL-6, IL-6 receptor, IL-10, and IL-12beta were investigated using the polymerase chain reaction-restriction fragment length polymorphism method. We then assessed the association between gene polymorphisms and postoperative infection.
  
  
  RESULTS
  Of the 110 patients studied, 18 (16%) developed a postoperative infection (pneumonia, 14 patients; pyothorax, 5; intraabdominal abscess, 1; neck abscess, 1; sepsis, 2). Although the characteristics of patients who developed postoperative infections did not differ, analysis of the genotypes using the Fisher exact test revealed a significantly (P = .0215) greater incidence of postoperative infections among those carrying the INF-gamma 874 (rs2430561) A/A and A/T genotypes. Moreover, univariate and multivariate logistic regression models showed patients carrying the INF-gamma 874A/T genotype were significantly more likely to develop postoperative infectious complications (odds ratio>3.4).
  
  
  CONCLUSIONS
  Our findings suggest that the IFN-gamma 874A>T polymorphism is potentially predictive of the likelihood that patients undergoing esophagectomy for thoracic esophageal cancer will develop postoperative infections. This polymorphism may therefore have important clinical relevance and should be considered when treatment regimens are designed.
authors.affiliation: Department of Surgery, Akita University School of Medicine, Akita, Japan. motoyama@doc.med.akita-u.ac.jp
authors.forename: Satoru
authors.initials: S
authors.lastname: Motoyama
authors.forename: Masatomo
authors.initials: M
authors.lastname: Miura
authors.forename: Yudai
authors.initials: Y
authors.lastname: Hinai
authors.forename: Kiyotomi
authors.initials: K
authors.lastname: Maruyama
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Usami
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Nakatsu
authors.forename: Hajime
authors.initials: H
authors.lastname: Saito
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Minamiya
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Murata
authors.forename: Toshio
authors.initials: T
authors.lastname: Suzuki
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Ogawa
mesh_terms: D000038:Abscess / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People; D004938:Esophageal Neoplasms / Q000601:surgery*; D016629:Esophagectomy / Q000009:adverse effects*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011183:Postoperative Complications / Q000209:etiology*; D012141:Respiratory Tract Infections / Q000235:genetics*; D018805:Sepsis / Q000235:genetics
chemical_list: D007371:Interferon-gamma