title: Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae.
abstract: Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100% and correctly identified 246/256 (96.69%) of the positive individuals, while ultrasound detected only 186/256 (73.44%). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick.
authors.affiliation: Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses e Serviço de Referência em Culicídeos Vetores, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, PE 50670-420, Brasil. rocha@cpqam.fiocruz.br
authors.forename: Abraham
authors.initials: A
authors.lastname: Rocha
authors.forename: Cynthia
authors.initials: C
authors.lastname: Braga
authors.forename: Marcela
authors.initials: M
authors.lastname: Belém
authors.forename: Arturo
authors.initials: A
authors.lastname: Carrera
authors.forename: Ana
authors.initials: A
authors.lastname: Aguiar-Santos
authors.forename: Paula
authors.initials: P
authors.lastname: Oliveira
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Texeira
authors.forename: André
authors.initials: A
authors.lastname: Furtado
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood*; D002648:Child; D004605:Elephantiasis, Filarial / Q000097:blood / Q000175:diagnosis* / Q000000981:diagnostic imaging; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008842:Microfilariae / Q000648:ultrastructure*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D014463:Ultrasonography; D014958:Wuchereria bancrofti / Q000276:immunology*; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth