Synthesis of 13C7-labeled iodoacetanilide and application to quantitative analysis of peptides and a protein by isotope differential mass spectrometry.
(13)C(7)-Labeled iodoacetanilide has been synthesized for specific labeling of sulfhydryl groups of cysteine residues and has been successfully applied to quantitative analysis of peptides and a commercial protein in combination with (13)C-unlabeled iodoacetanilide and a MALDI mass spectrometer. Subsequent tandem mass spectrum analysis revealed that (13)C(7)-labeled iodoacetanilide remained intact during the collision-induced dissociation (CID) conditions.
Niwayama
Satomi
S
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA. satomi.niwayama@ttu.edu
Zabet-Moghaddam
Masoud
M
Kurono
Sadamu
S
Cho
Hanjoung
H
D000083:Acetanilides / Q000138:chemical synthesis* / Q000737:chemistry; D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry; D002417:Cattle; D007553:Isotope Labeling; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000083:Acetanilides; D002247:Carbon Isotopes; D010455:Peptides; D011506:Proteins; C545111:iodoacetanilide; D012710:Serum Albumin, Bovine