title: Severe pandemic (H1N1) 2009 influenza with false negative direct fluorescent antibody assay: case series.
abstract: Between May and June of 2009 we observed 4 patients that presented with severe influenza-like symptoms and respiratory failure. All cases tested negative for influenza A and B by direct fluorescent antibody assay. Further investigation revealed all cases to be positive for pandemic (H1N1) 2009 influenza virus by real-time RT-PCR. This article includes a description of these cases and the characteristics associated with them.
authors.affiliation: Division of Infectious Diseases, Mount Sinai School of Medicine, New York, NY 10029, United States. luciano.kapelusznik@mssm.edu
authors.forename: Luciano
authors.initials: L
authors.lastname: Kapelusznik
authors.forename: Rupa
authors.initials: R
authors.lastname: Patel
authors.forename: Jennifer
authors.initials: J
authors.lastname: Jao
authors.forename: Gopi
authors.initials: G
authors.lastname: Patel
authors.forename: Simon
authors.initials: S
authors.lastname: Daefler
authors.forename: Vincent
authors.initials: V
authors.lastname: Labombardi
authors.forename: David
authors.initials: D
authors.lastname: Calfee
mesh_terms: D000328:Adult; D000368:Aged; D005188:False Negative Reactions; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*