[Expression of R-spondin1 in intestinal epithelium of mice with intestinal ischemia-reperfusion injury].
OBJECTIVE
To investigate the expression of R-spondin1 (RSpo1) in the intestinal epithelium of mice with intestinal ischemia-reperfusion injury and explore its significance.


METHODS
Fifty normal male Kunming mice were randomized into sham-operated group (n=10) and intestinal ischemia-reperfusion injury group (n=40), and in the latter group, the mice were subjected to 20-min intestinal mesenteric artery occlusion followed by reperfusion for 6, 12, 24, or 48 h. Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to detect intestinal RSpo1 expression of the mice.


RESULTS
The results of RT-PCR and ELISA showed that RSpo1 expression was significantly decreased in mice at 6 h of reperfusion following the intestinal ischemia (P<0.05), and increased gradually with prolonged repersuion time, reaching the peak level at 24 h (P<0.05). The expression underwent rapid decrease afterwards to a significantly lower level than that in the control group at 48 h (P<0.05).


CONCLUSIONS
Intestinal ischemia-reperfusion injury may inhibit expression of RSpo1 in the early stage, and enhance its expression in the middle stage. RSpo1 can promote proliferation and differentiation of intestinal epithelial stem cells and plays an important role in the repair intestinal mucosal damage.
Yin
Gang
G
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. yg157@163.com
Wu
Cheng-Tang
CT
Wang
Yun-Xing
YX
Lei
Shang-Tong
ST
D000818:Animals; D049109:Cell Proliferation; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007422:Intestines / Q000098:blood supply*; D008297:Male; D051379:Mice; D011897:Random Allocation; D015427:Reperfusion Injury / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*; D019699:Thrombospondins / Q000235:genetics / Q000378:metabolism*
C518903:RSPO1 protein, mouse; D019699:Thrombospondins