An interference study of the copper-induced plasma oxidizability test.
OBJECTIVE
The aim of this study is to investigate the effect of cristaloid cardioplegic fluid (CCF) and its contents on the susceptibility of plasma to copper-catalyzed lipid peroxidation test.


METHODS
The plasma pool was divided into eight groups. Equal volumes of CCF or one of its contents were added to each group of the plasma pool. The accumulation of conjugated diene (CD) by copper-induced oxidation was monitored for a period of 5 h. Thiobarbituric acid-reactive substances (TBARS) formed during the incubation of plasma with copper was also measured.


RESULTS
It was found that, the production of CD and TBARS were inhibited and the lag time had increased, when the plasma was mixed with CCF or its contents.


CONCLUSIONS
As a result, we conclude that that the susceptibility of plasma to copper-induced lipid peroxidation is interfered by CCF. The chloride ions, which major content of CCF, may play an important role on this effect.
Eskiocak
Sevgi
S
Department of Biochemistry, University of Trakya, Faculty of Medicine, Edirne, Turkey. drseskiocak@yahoo.co.uk
Ege
Turan
T
Yapar
Suleyman Bedir
SB
Kaya
Sabriye
S
Sayilan
Gulben
G
D000328:Adult; D003300:Copper / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper