[Effects and mechanism of decorin on the proliferation of HuH7 hepatoma carcinoma cells in vitro].
OBJECTIVE
To investigate the effects and mechanism of decorin( DCN) on the proliferation of HuH7 hepatoma carcinoma cell line in vitro.


METHODS
Hepatoma carcinoma cells was cultured with DCN in different concentration (0, 25, 50, 75, 100, 125, 150, 200 microg/L) for different time(12, 24, 48, 72 h and 2 weeks). Cell activities were studied by MTT and clone test. The changes of cell cycle and apoptosis were analyzed by Flow cytometry.


RESULTS
The proliferation of HuH7 cells could be inhibited by DCN in vitro and the inhibition effect was the time and dose dependent relationship. DCN could block cell cycle at G(1); phase. Apoptosis of hepatocarcinoma cells could be efficiently induced by DCN in a time/dose-dependent manner.


CONCLUSIONS
DCN may be a negative regulatory protein inhibiting hepatoma carcinoma cell proliferation through inhibiting cell cycle and inducing apoptosis of cell in vitro.
Shangguan
Jian-ying
JY
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Dou
Ke-feng
KF
Li
Xiao
X
Hu
Xiao-jun
XJ
Zhang
Fu-qin
FQ
Yong
Zhao-sheng
ZS
Ti
Zhen-yu
ZY
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000503:physiopathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D011509:Proteoglycans / Q000378:metabolism*
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans