title: Superantigen-presentation by rat major histocompatibility complex class II molecules RT1.Bl and RT1.Dl.
abstract: Rat major histocompatibility complex (MHC) class II molecules RT1.B(l) (DQ-like) and RT1.D(l) (DR-like) were cloned from the LEW strain using reverse transcription-polymerase chain reaction and expressed in mouse L929 cells. The transduced lines bound MHC class II-specific monoclonal antibodies in an MHC-isotype-specific manner and presented peptide antigens and superantigens to T-cell hybridomas. The T-cell-hybridomas responded well to all superantigens presented by human MHC class II, whereas the response varied considerably with rat MHC class II-transduced lines as presenters. The T-cell hybridomas responded to the pyrogenic superantigens Staphylococcus enterotoxin B (SEB), SEC1, SEC2 and SEC3 only at high concentrations with RT1.B(l)-transduced and RT1.D(l)-transduced cells as presenters. The same was true for streptococcal pyrogenic exotoxin A (SPEA), but this was presented only by RT1.B(l) and not by RT1.D(l). SPEC was recognized only if presented by human MHC class II. Presentation of Yersinia pseudotuberculosis superantigen (YPM) showed no MHC isotype preference, while Mycoplasma arthritidis superantigen (MAS or MAM) was presented by RT1.D(l) but not by RT1.B(l). Interestingly, and in contrast to RT1.B(l), the RT1.D(l) completely failed to present SEA and toxic shock syndrome toxin 1 even after transduction of invariant chain (CD74) or expression in other cell types such as the surface MHC class II-negative mouse B-cell lymphoma (M12.4.1.C3). We discuss the idea that a lack of SEA presentation may not be a general feature of RT1.D molecules but could be a consequence of RT1.D(l)beta-chain allele-specific substitutions (arginine 80 to lysine, asparagine 82 to aspartic acid) in the extremely conserved region flanking the Zn(2+)-binding histidine 81, which is crucial for high-affinity SEA-binding.
authors.affiliation: Institute for Virology and Immunobiology, Julius-Maximilians-University Würzburg, Würzburg, Germany.
authors.forename: Henry
authors.initials: H
authors.lastname: Dlaske
authors.forename: Hatice
authors.initials: H
authors.lastname: Karaüzüm
authors.forename: Elisa
authors.initials: E
authors.lastname: Monzon-Casanova
authors.forename: Ronald
authors.initials: R
authors.lastname: Rudolf
authors.forename: Lisa
authors.initials: L
authors.lastname: Starick
authors.forename: Ingrid
authors.initials: I
authors.lastname: Müller
authors.forename: Gerhild
authors.initials: G
authors.lastname: Wildner
authors.forename: Maria
authors.initials: M
authors.lastname: Diedrichs-Möhring
authors.forename: Norbert
authors.initials: N
authors.lastname: Koch
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.forename: Takehiko
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Kurt
authors.initials: K
authors.lastname: Wonigeit
authors.forename: Bernhard
authors.initials: B
authors.lastname: Fleischer
authors.forename: Silke
authors.initials: S
authors.lastname: Overbeck
authors.forename: Lothar
authors.initials: L
authors.lastname: Rink
authors.forename: Thomas
authors.initials: T
authors.lastname: Herrmann
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D002460:Cell Line; D005347:Fibroblasts / Q000276:immunology; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D051379:Mice; D051381:Rats; D011917:Rats, Inbred Lew; D018089:Superantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014161:Transduction, Genetic
chemical_list: D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D018089:Superantigens; C038353:histocompatibility antigens RT, rat