title: Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro.
abstract: This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 microg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85%), 2,2-azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77%), hydroxyl (70%), superoxide (76%), nitric oxide (78%) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57%). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiation-induced micronuclei, with a maximum effect at 125 microg/mL CCE for 1 h before 2 Gy of radiation. Similarly, there was a significant (P < 0.05-0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125 microg/mL) before exposure to different doses of gamma radiation, that is, 0.5-4 Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential.
authors.affiliation: Division of Radiobiology & Toxicology, Manipal Life Sciences Center, Manipal University, Karnataka, India. satishraomlsc@gmail.com
authors.forename: B S S
authors.initials: BS
authors.lastname: Rao
authors.forename: R
authors.initials: R
authors.lastname: Shanbhoge
authors.forename: B N
authors.initials: BN
authors.lastname: Rao
authors.forename: S K
authors.initials: SK
authors.lastname: Adiga
authors.forename: D
authors.initials: D
authors.lastname: Upadhya
authors.forename: B K
authors.initials: BK
authors.lastname: Aithal
authors.forename: M R S
authors.initials: MR
authors.lastname: Kumar
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D031743:Cymbopogon / Q000737:chemistry*; D004249:DNA Damage / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D000431:Ethanol; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D005720:Gamma Rays; D015162:Micronucleus Tests; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000494:pharmacology; D011837:Radiation-Protective Agents; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014867:Water
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D012997:Solvents; D014867:Water; D000431:Ethanol