Application of a genome walking method for the study of the spinach Lhcb1 multigene family.
We describe the application of a novel genome walking (GW) strategy for the one-shot identification of members of multigene families. The method was used to study the spinach Lhcb1 family (encoding the light harvesting complex protein Lhcb1), for which three cDNAs were known. Two additional genes and regulatory regions of the five members of the family were identified. For one of the newly detected genes, sequencing of full-length cDNA and analysis of regulatory regions by gel-shift are also reported. To our best knowledge, this is the first report on the use of a GW approach for the study of a multigene family.
Leoni
Claudia
C
Department of Biochemistry and Molecular Biology, University of Bari, Via Amendola 165/A, 70126 Bari, Italy.
Volpicella
Mariateresa
M
Placido
Antonio
A
Gallerani
Raffaele
R
Ceci
Luigi R
LR
D015139:Blotting, Southern; D016386:Chromosome Walking / Q000379:methods*; D024202:Electrophoretic Mobility Shift Assay; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics*; D018724:Spinacia oleracea / Q000235:genetics*