Proteomic characterization of midproliferative and midsecretory human endometrium.
This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIalpha, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.
Chen
Jenny I C
JI
Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Victoria 3168, Australia. jenny.chen@princehenrys.org
Hannan
Natalie J
NJ
Mak
Yunxian
Y
Nicholls
Peter K
PK
Zhang
Jin
J
Rainczuk
Adam
A
Stanton
Peter G
PG
Robertson
David M
DM
Salamonsen
Lois A
LA
Stephens
Andrew N
AN
D049109:Cell Proliferation; D015180:Electrophoresis, Gel, Two-Dimensional; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome