[Anti-inflammatory effect of near-infrared irradiated cell culture media].
BACKGROUND
Near-infrared light (NIR, 0.8-1.5 microm light) has been used in therapeutic devices for various injuries such as infected, ischemic and hypoxic wound. NIR-emitting technology has been developed recently in Korea. We hypothesized that NIR may have an anti-inflammatory effect and investigated the effect of NIR-irradiated media on cell culture.


METHODS
Three kinds of cell lines, CAPE (vascular endothelial cell), NIH3T3 (fibroblast), and RD (smooth muscle cell) cells were cultured for 4 days in 10% FBS-containing media (1 x 10(4) cells/well), which were irradiated or not irradiated (control) by Eco-NFIR Drive (Model #0210, Ecowavetech, Korea). The cells were stimulated by 10 mcg/mL of bacterial lipopolysaccharide (LPS) or sodium nitroprusside (SNP). Cellular proliferation was measured by methylthiazol tetrazolium assay. Expression of interleukin (IL)-1 beta and nitric oxide was measured by ELISA. Expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was measured by immunofluorescence staining.


RESULTS
NIR-irradiated medium was favorable for CAPE cell proliferation (N=8, P=0.000). IL-1 beta secretion from LPS-stimulated NIH3T3 cells incubated in the NIR medium was below that of control medium (N=4, P=0.026). Nitrate production seemed to be low in NIR-irradiated medium although statistically insignificant (N=4, P=0.076). Expression of iNOS of the LPS-stimulated cells was decreased in NIR medium, however, Cox-2 expression was not different between the two media.


CONCLUSIONS
NIR-irradiated medium supported vascular endothelial cell proliferation and showed an anti-inflammatory effect on fibroblast culture. These results can be used as basic data for future research on the clinical application of NIR.
Kim
Sang-Gyung
SG
Department of Laboratory Medicine, Catholic University of Daegu, School of Medicine, Daegu, Korea. sgkim@cu.ac.kr
Shin
Im-Hee
IH
Choi
Chang-Hyuk
CH
Choe
Jung-Yoon
JY
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D002417:Cattle; D002460:Cell Line; D003470:Culture Media; D051546:Cyclooxygenase 2 / Q000378:metabolism; D006801:Humans; D007259:Infrared Rays; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D003470:Culture Media; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2