[Study on interaction between SARS-CoV N and MAP19].
OBJECTIVE
Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N)protein of SARS-CoV as a bait, the N protein was found to interact with MAP19, a non-enzymatic protein of MASP(mannan-associated serine protease). The interaction between SARS-CoV N and MAP19 would be further tested in cells in this article.


METHODS
The interaction between SARS-CoV N and MAP19 was demonstrated by immuno- coprecipitation, and the amount of MAP19 influenced by SARS-CoV N was investgated by Western blot.


RESULTS
The interaction between SARS-CoV N and MAP19 was already demonstrated by immuno-coprecipitation. SARS-CoV N greatly increased the amount of MAP19.


CONCLUSIONS
SARS-CoV N can bind with MAP19 in cells. Our study may be conductive to further research into the molecular mechanism of action between SARS-CoV N and MAP19.
Liu
Jun-li
JL
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China. liujunli306@sohu.com
Cao
Cheng
C
Ma
Qing-jun
QJ
D002460:Cell Line; D000086462:Coronavirus Nucleocapsid Proteins; D006801:Humans; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000235:genetics / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*; D045169:Severe Acute Respiratory Syndrome / Q000201:enzymology / Q000378:metabolism* / Q000821:virology
D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; C105296:MASP2 protein, human; D050606:Mannose-Binding Protein-Associated Serine Proteases