Use of a bisphenol-A imprinted polymer as a selective sorbent for the determination of phenols and phenoxyacids in honey by liquid chromatography with diode array and tandem mass spectrometric detection.
An extraction-preconcentration procedure based on the use of a molecularly imprinted polymer (MIP) as selective sorbent has been developed for the determination of several phenolic compounds (bisphenol-A, bisphenol-F and 4-nitrophenol) and phenoxyacid herbicides (2,4-D, 2,4,5-T and 2,4,5-TP) in honey samples. Liquid chromatography with diode array detection (LC-DAD) and electrospray ionisation-ion trap mass spectrometry (LC-IT-MS) were used for the separation, identification and quantification of these analytes. The molecularly imprinted polymer was obtained by precipitation polymerisation with bisphenol-A (BPA) as template and 4-vinylpyridine as the functional monomer. The behaviour of this sorbent was compared with those of other materials frequently used in SPE. The selectivity of the BPA-MIP for the target analytes was tested in samples containing other pesticides in common use. The recoveries achieved for all six compounds were in the 81-96% range. By applying the proposed procedure prior to LC-IT-MS, the limits of detection achieved in commercial honey samples were in the 0.1-3.8 ng g(-1) range, with relative standard deviations of 12-24%.
Herrero-Hernández
E
E
Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Química, Universidad de Salamanca, Plaza de la Merced s/n, 37008 Salamanca, Spain.
Carabias-Martínez
R
R
Rodríguez-Gonzalo
E
E
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000032:analysis; D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis; D001559:Benzhydryl Compounds; D002853:Chromatography, Liquid / Q000379:methods*; D006540:Herbicides / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D054802:Molecular Imprinting; D009596:Nitrophenols / Q000032:analysis; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
D015085:2,4,5-Trichlorophenoxyacetic Acid; D015084:2,4-Dichlorophenoxyacetic Acid; D001559:Benzhydryl Compounds; D006540:Herbicides; D009596:Nitrophenols; D010636:Phenols; D011108:Polymers; D011725:Pyridines; C009264:fenoprop; C029351:4-vinylpyridine; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C024836:4-nitrophenol