Effect of blockade of tumor necrosis factor-alpha with etanercept on surgical wound healing in SWISS-OF1 mice.
OBJECTIVE
To assess whether blockade of tumor necrosis factor-alpha (TNF-alpha) influences surgical wound healing in a normal mouse experimental model.


METHODS
Wound healing time course and degree of surgical wound collagenization were measured by morphological techniques and digital image analysis in 80 male SWISS-OF1 mice (40 received subcutaneous etanercept at a dose of 0.1 mg/25 g/ at -7, 0, 7, and 14 days).


RESULTS
No significant differences were observed between treated and untreated animals in wound healing, re-epithelialization, or formation of inflammatory infiltrate or granulation tissue at days 7, 15, or 20 after surgery. At 20 days, the collagen area was larger in treated versus untreated mice (109,029 +/- 28,489 microm(2) vs 79,305 +/- 19,798 microm(2), p = 0.026, Mann-Whitney U test).


CONCLUSIONS
Surgical wounds showed a higher degree of collagenization at 20 days in etanercept-treated versus untreated mice, with no differences in the time course of wound healing. These data suggest that biological therapies to block TNF-alpha do not affect wound healing and do not need to be suspended during the perioperative period.
Iglesias
Estibaliz
E
Department of Rheumatology, University Hospital of Granada, and University of Granada, Granada, Spain.
O'Valle
Francisco
F
Salvatierra
Juan
J
Aneiros-Fernández
Jose
J
Cantero-Hinojosa
Jesus
J
Hernández-Cortés
Pedro
P
D000818:Animals; D018501:Antirheumatic Agents / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D000068800:Etanercept; D013502:General Surgery; D006097:Granulation Tissue / Q000187:drug effects; D007074:Immunoglobulin G / Q000494:pharmacology*; D008297:Male; D051379:Mice; D023421:Models, Animal; D019990:Perioperative Care; D018124:Receptors, Tumor Necrosis Factor; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D018501:Antirheumatic Agents; D007074:Immunoglobulin G; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen; D000068800:Etanercept