title: Colonisation of poplar trees by gfp expressing bacterial endophytes.
abstract: With the exception of nitrogen fixing bacteria, there is little known about the colonisation patterns or population sizes of bacterial endophytes in deciduous trees. This study describes the isolation, identification, construction and re-colonisation patterns of three green fluorescent protein(gfp):kanamycin(R) labelled bacterial endophytes when re-introduced into poplar trees, their original host plant. Two of these endophytes showed considerable colonisation in the roots and stems of inoculated plants. gfp expressing cells of all three strains were observed to colonise the xylem tissue of the root. All three strains proved to be efficient rhizosphere colonisers, supporting the theory that the rhizosphere can serve as a source of bacterial endophytes.
authors.affiliation: Department of Applied Biology and Chemistry, Institute of Technology, Carlow, Ireland.
authors.forename: Kieran
authors.initials: K
authors.lastname: Germaine
authors.forename: Elaine
authors.initials: E
authors.lastname: Keogh
authors.forename: Guiomar
authors.initials: G
authors.lastname: Garcia-Cabellos
authors.forename: Brigitte
authors.initials: B
authors.lastname: Borremans
authors.forename: Daniel
authors.initials: D
authors.lastname: Lelie
authors.forename: Tanja
authors.initials: T
authors.lastname: Barac
authors.forename: Licy
authors.initials: L
authors.lastname: Oeyen
authors.forename: Jaco
authors.initials: J
authors.lastname: Vangronsveld
authors.forename: Fiona Porteous
authors.initials: FP
authors.lastname: Moore
authors.forename: Edward R B
authors.initials: ER
authors.lastname: Moore
authors.forename: Colin D
authors.initials: CD
authors.lastname: Campbell
authors.forename: David
authors.initials: D
authors.lastname: Ryan
authors.forename: David N
authors.initials: DN
authors.lastname: Dowling
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D044822:Biodiversity; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007613:Kanamycin Resistance; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013194:Staining and Labeling / Q000379:methods; D052584:Xylem / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins