title: Survival and virulence of Flavobacterium psychrophilum in water microcosms.
abstract: Flavobacterium psychrophilum, the causative agent of rainbow trout fry syndrome and cold water disease in salmonids, causes serious disease outbreaks in fish farms worldwide. The aim of the present study was to examine the survival capacity of F. psychrophilum in laboratory microcosms containing sterilised water under different environmental conditions and to examine the virulence of starving F. psychrophilum cells. The results showed that F. psychrophilum survived for very long time in sterilised fresh water at 15 degrees C and the cells were still culturable after starvation for 300 days. A high salinity of the water (30 per thousand) drastically reduced the number of culturable cells below detection limit after incubation for 1 day. A water salinity of approximately 6 per thousand initially reduced the number of culturable cells below the detection limit, but cells were again recovered on agar plates at the end of the experiment. The presence of sediment containing nutrients in the experimental water microcosms increased the survival of F. psychrophilum. The challenge experiments indicated that the virulence of starving F. psychrophilum is maintained for at least seven days after the transfer of the bacterial cells to fresh water.
authors.affiliation: Laboratory of Aquatic Pathobiology and Environmental and Marine Biology, Department of Biology, Abo Akademi University, BioCity, Artillerig. 6, 20520 Abo, Finland.
authors.forename: Jari
authors.initials: J
authors.lastname: Madetoja
authors.forename: Staffan
authors.initials: S
authors.lastname: Nystedt
authors.forename: Tom
authors.initials: T
authors.lastname: Wiklund