Partial nitritation treatment of underground brine waste with high ammonium and salt content.
Underground brine waste containing high concentrations of ammonium and with a salinity of 3% is usually generated during the production of methane gas and iodine in the gas field of Chiba Prefecture, Japan. In this study, one swim-bed reactor, packed with a novel acrylic fiber biomass carrier (Biofringe), was applied to the partial nitritation treatment of this kind of underground brine waste. A stable nitrite production rate of 1.6 kg NO(2)-N m(-3) d(-1) was obtained under a nitrogen loading rate of 3.0 kg-N m(-3) d(-1), at a pH of 7.5 and a temperature of 25 degrees C. Nitrate production was negligible and the effluent NO(2)-N/NO(x)-N ratio was above 98% due to the successful inhibition of nitrite-oxidizing bacterial activity. Free ammonia was considered to be the main factor for inhibiting the activity of nitrite-oxidizing bacteria. A microbial community shift was demonstrated by 16S rRNA analysis, and it was shown that the ammonium-oxidizing bacteria became the predominant species after successful nitrite accumulation was observed.
Shinohara
Takehiko
T
Kumamoto University, Kurokami, Japan.
Qiao
Sen
S
Yamamoto
Taichi
T
Nishiyama
Takashi
T
Fujii
Takao
T
Kaiho
Tatsuo
T
Bhatti
Zafar
Z
Furukawa
Kenji
K
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000032:analysis* / Q000737:chemistry; D012965:Sodium Chloride / Q000032:analysis; D014865:Waste Disposal, Fluid / Q000379:methods; D018508:Water Purification / Q000379:methods
D009566:Nitrates; D009573:Nitrites; D000644:Quaternary Ammonium Compounds; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts; C017082:brine; D012965:Sodium Chloride