title: Superior long-term stability of a glucose biosensor based on inserted barrel plating gold electrodes.
abstract: Disposable one shot usage blood glucose strips are routinely used in the diagnosis and management of diabetes mellitus and their performance can vary greatly. In this paper we critically evaluated the long-term stability of glucose strips made of barrel plating gold electrodes. Compared to other glucose biosensing platforms of vapor deposited palladium and screen printed carbon electrodes, the proposed glucose biosensor was found to show the best stability among the three biosensing platforms in thermal acceleration experiments at 40 degrees C for 6 months with an average bias of 3.4% at glucose concentrations of 5-20 mM. The precision test of this barrel plating gold glucose biosensor also showed the best performance (coefficients of variation in the range of 1.4-2.4%) in thermal acceleration experiments at 40 degrees C, 50 degrees C and 70 degrees C for 27 days. Error grid analysis revealed that all measurements fell in zone A and zone B. Regression analysis showed no significant difference between the proposed biosensor and the reference method at 99% confidence level. The amperometric glucose biosensor fabricated by inserting two barrel plating gold electrodes onto an injection-molding plastic base followed by immobilizing with a bio-reagent layer and membrane was very impressive with a long-term stability up to 2.5 years at 25 degrees C. Overall, these results indicated that the glucose oxidase/barrel plating gold biosensing platform is ideal for long-term accurate glycemic control.
authors.affiliation: Department of Core Technical Research, Bionime Corporation, Taichung 412, Taiwan. brown.hsu@bionime.com
authors.forename: Cheng-Teng
authors.initials: CT
authors.lastname: Hsu
authors.forename: Hung-Chan
authors.initials: HC
authors.lastname: Hsiao
authors.forename: Mei-Yen
authors.initials: MY
authors.lastname: Fang
authors.forename: Jyh-Myng
authors.initials: JM
authors.lastname: Zen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004795:Enzyme Stability; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose