title: Infrared-based third and second harmonic generation imaging of cornea.
abstract: The cornea functions as an optical lens and plays an important role in vision. For corneal diagnosis and treatment such as refractive surgery, a microscopic imaging system with a 3-D cellular resolution and retinal safety is strongly desired. Recently, confocal and multiphoton microscopies have been applied to corneal imaging with visible to near-infrared light sources. To increase retinal safety, an infrared light source is be needed. In this work, an infrared-based third and second harmonic generation microscopic study of mouse eyes is reported with approximately 700-mum penetrability and high cellular resolution. This study provides a critical reference for future development of infrared-based corneal imaging.
authors.affiliation: National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Department of Electrical Engineering, No 1 Roosevelt Road Section 4, Taipei 10617, Taiwan.
authors.forename: Szu-Yu
authors.initials: SY
authors.lastname: Chen
authors.forename: Han-Chieh
authors.initials: HC
authors.lastname: Yu
authors.forename: I-Jong
authors.initials: IJ
authors.lastname: Wang
authors.forename: Chi-Kuang
authors.initials: CK
authors.lastname: Sun
mesh_terms: D000818:Animals; D003315:Cornea / Q000166:cytology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007259:Infrared Rays; D008029:Lighting / Q000295:instrumentation*; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration