Application of 2D-DIGE in cancer proteomics toward personalized medicine.
Two-dimensional difference gel electrophoresis (2D-DIGE) is an advanced variation of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE); protein samples are labeled with different fluorescent dyes, mixed and separated by 2D-PAGE. 2D-DIGE solves major inherent drawbacks of 2D-PAGE, demonstrating great utility in biomarker studies. Biomarker development requires quantitative, reproducible, highly sensitive and high-throughput experimental platforms, and 2D-DIGE meets these criteria. Here we demonstrate the advantages of 2D-DIGE and discuss the possibilities 2D-DIGE offers for further, more comprehensive proteome studies.
Kondo
Tadashi
T
National Cancer Center Research Institute, Proteome Bioinformatics Project, Tokyo, Japan.
Hirohashi
Setsuo
S
D014408:Biomarkers, Tumor / Q000032:analysis*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005456:Fluorescent Dyes; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000737:chemistry*; D057285:Precision Medicine / Q000379:methods; D040901:Proteomics / Q000379:methods* / Q000706:statistics & numerical data
D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins