Ethyl alcohol extracts of Hizikia fusiforme sensitize AGS human gastric adenocarcinoma cells to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis.
Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis has been reported in some cancer cells, including AGS human gastric adenocarcinoma cells. Hizikia fusiforme is a commonly used brown seaweed species in Korea that possesses potent antibacterial, antifungal, and anti-inflammatory activities. In this study, we demonstrated that treatment with TRAIL in combination with subtoxic concentrations of ethyl alcohol extract of H. fusiforme (EAHF) sensitized TRAIL-resistant AGS cells to TRAIL-mediated apoptosis. Combined treatment with EAHF and TRAIL increased chromatin condensation, DNA fragmentation, and sub-G1-phase DNA content. The restored sensitivity to TRAIL-induced apoptosis appeared to be correlated with the modulation of Bcl-2 family proteins and activation of caspases, which resulted in the cleavage of poly(ADP-ribose)polymerase. Taken together, the use of EAHF in combination with TRAIL may be an effective and selective anticancer strategy via suppressing the resistance to TRAIL-induced apoptosis in some tumor cell lines, including AGS cells.
Kim
Tae-Yeon
TY
Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Jeju, Republic of Korea.
Jin
Cheng-Yun
CY
Kim
Gi-Young
GY
Choi
Il-Whan
IW
Jeong
Yong Kee
YK
Nam
Taek-Jeong
TJ
Kim
Se-Kwon
SK
Choi
Yung Hyun
YH
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004359:Drug Therapy, Combination; D006801:Humans; D007723:Korea; D008024:Ligands; D000459:Phaeophyceae; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology / Q000627:therapeutic use*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D002843:Chromatin; D008024:Ligands; D010936:Plant Extracts; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D051028:bcl-2-Associated X Protein; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases