title: High-level resistance of Nosema ceranae, a parasite of the honeybee, to temperature and desiccation.
abstract: Resistance of Nosema ceranae to different exposure conditions has been evaluated by using Sytox green and DAPI (4',6-diamidino-2-phenylindole) to test spore viability. High thermotolerance at 60 and 35 degrees C and resistance to desiccation were observed. However, a significant decrease in viability after freezing and a rapid degeneration of spores maintained at 4 degrees C were also detected.
authors.affiliation: Laboratorio de Parasitología, Facultad de Farmacia, Universidad San Pablo CEU, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Fenoy
authors.forename: C
authors.initials: C
authors.lastname: Rueda
authors.forename: M
authors.initials: M
authors.lastname: Higes
authors.forename: R
authors.initials: R
authors.lastname: Martín-Hernández
authors.forename: C
authors.initials: C
authors.lastname: del Aguila
mesh_terms: D000818:Animals; D001516:Bees / Q000382:microbiology; D003890:Desiccation; D005615:Freezing; D006358:Hot Temperature; D007211:Indoles / Q000378:metabolism; D050296:Microbial Viability; D016818:Nosema / Q000302:isolation & purification / Q000502:physiology* / Q000528:radiation effects*; D009930:Organic Chemicals / Q000378:metabolism; D013172:Spores, Fungal / Q000502:physiology / Q000528:radiation effects; D013194:Staining and Labeling / Q000379:methods; D013312:Stress, Physiological
chemical_list: D007211:Indoles; D009930:Organic Chemicals; C402795:SYTOX Green; C007293:DAPI