title: [Construction of eukaryotic expression vector containing foot-and-mouth disease virus VP1 gene and its expression in dendritic cells].
abstract: OBJECTIVE
  To construct the eukaryotic expression vectors for the FMDV VP1 gene and transfect dendritic cells(DC) for the expression of VP1.
  
  
  METHODS
  The plasmid pMD-18-VP1 was subcloned into pcDNA3.1(+) to construct recombinant eukaryotic expression plasmids named as pcDNA3.1-VP1. The recombinant plasmids were transfected into DC by lipofectamine method and the positive cell clones were screened with G418. The expression of FMDV VP1 gene in DC was determined by Western blot.
  
  
  RESULTS
  The correct construction of pcDNA3.1-VP1 was identified by means of restriction enzyme analysis and nucleotide sequence determination. It was showed by SDS-PAGE and Western blot that transfected cells, DC, expressed FMDV VP1 gene constantly.
  
  
  CONCLUSIONS
  The pcDNA3.1-VP1 eukaryotic expression plasmids are successfully constructed and VP1 gene can be sustainly expressed in DC.
authors.affiliation: Department of Immunology and Cell Biology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China. lijie2006698@yahoo.com.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Ruo
authors.initials: R
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Bian
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jia-xin
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000276:immunology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus