Miniaturized metal semiconductor metal photocurrent system for biomolecular sensing via chemiluminescence.
A miniaturized metal semiconductor metal photodetector was developed as the core detector for chemiluminescence biosensor. The biosensor utilized the semiconductor manufacturing to fabricate the 83 interdigitated patterns of 250-nm metal line and 278-nm space in 100 microm x 100 microm active region. The established real-time detector was operated at 0.4 V to ensure the maximal signal to background ratio of 3600 under illumination intensity of 1.46 mW/cm(2). A chemiluminescence in the miniaturized chamber was successfully proposed to determine the model protein concentration in real-time analysis. Before the emission of light from the catalytic reaction of substrate, the model protein of streptavidin bound to horseradish peroxidase was successfully immobilized onto the sensor surface through the high-affinity conjugate with biotin. The detection limit of 4.76 nM for streptavidin analysis was obtained under the calibration curve of linear range over 5-100 nM with correlation coefficient of 0.9999.
Lin
Chie-Chieh
CC
Department of Materials Science and Engineering, Institute of Nanotechnology, National Chiao Tung University, Hsinchu, Taiwan.
Ko
Fu-Hsiang
FH
Chen
Chun-Chi
CC
Yang
Yuh-Shyong
YS
Chang
Feng-Chi
FC
Wu
Chung-Shu
CS
D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002138:Calibration; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000032:analysis / Q000378:metabolism; D055767:Immobilized Proteins / Q000032:analysis / Q000378:metabolism; D016014:Linear Models; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D008904:Miniaturization / Q000295:instrumentation*; D008956:Models, Chemical; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012666:Semiconductors / Q000295:instrumentation; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D055767:Immobilized Proteins; D011506:Proteins; C058865:Strep-avidin conjugated horseradish peroxidase; D006735:Horseradish Peroxidase