title: [Introduction to the standard operation program of spoligotyping on mycobacterium tuberculosis in China].
abstract: OBJECTIVE
  To study and preliminarily evaluate the standard spacer oligonucleotide typing (Spoligotyping) method and the application on Mycobacterium tuberculosis (M. tuberculosis).
  
  
  METHODS
  Spoligotyping on 224 M. tuberculosis strains was studied by the molecular biological techniques, including DNA isolation, PCR, reverse line blot hybridization, together with data analysis software BioNumerics (Version 5.0).
  
  
  RESULTS
  Standardization on both Spoligotyping method and parameters in result analysis and the usage of the analysis software were studied. Through this method, 224 M. tuberculosis clinical strains were classified into 2 clusters including 129 Beijing family strains and 95 non-Beijing family strains. The predominant strains belonged to Beijing family.
  
  
  CONCLUSIONS
  Standard Spoligotyping method was preliminary determined in China, showing that it was a simple, rapid, and robust method for simultaneous detection and typing of M. tuberculosis. This method can be used for tracing the source of infection and understanding the epidemic trend of M. tuberculosis. Spoligotyping can also be served as a method for simultaneous detection and typing of M. tuberculosis, and to identify Beijing family strains.
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Dong
authors.forename: Bing
authors.initials: B
authors.lastname: Lu
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Zhi-guang
authors.initials: ZG
authors.lastname: Liu
authors.forename: Xiu-qin
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Kang-lin
authors.initials: KL
authors.lastname: Wan
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015345:Oligonucleotide Probes
chemical_list: D015345:Oligonucleotide Probes