Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering.
We present real-time, rapid detection of Mycoplasma pneumonia in PBS inside a Y-channel PDMS microfluidic device via optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed with serially diluted M. pneumonia solutions using highly carboxylated polystyrene particles of 390 and 500 nm diameter conjugated with monoclonal anti-M. pneumonia. Proximity optical fibers were located around the viewing cell of the device, which were used to measure the increase in 45 degrees forward light scattering of the aggregated particles. The detection limit are slightly less than 50 pg mL(-1) both for 390 and 500 nm microspheres and the detection time do not exceed 90 s.
Kim
Keesung
K
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea.
Jung
Ho-Sup
HS
Song
Jae-Young
JY
Lee
Man-Ryul
MR
Kim
Kye-Seong
KS
Suh
Kahp-Yang
KY
D055910:Antibodies, Immobilized / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018929:Cell Culture Techniques; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005336:Fiber Optic Technology; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007841:Latex Fixation Tests / Q000295:instrumentation / Q000379:methods*; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification* / Q000378:metabolism; D009757:Nylons / Q000737:chemistry; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer