Diacerein decreases TNF-alpha and IL-1beta levels in peritoneal fluid and prevents Baker's yeast-induced fever in young rats.
OBJECTIVE
To investigate the effect of diacerein, an anti-inflammatory drug, on body temperature and protocols of fever induction in male Wistar rats.


METHODS
The effect of diacerein (5.0 mg/kg, s.c.) on rectal temperature (T (R)) changes induced by Baker's yeast (0.135 g/kg, i.p.) and PGE(2) (10 ng/animal, i.t.) was evaluated. T (R) changes were recorded over time. The leukocyte count and TNF-alpha and IL-1beta content were evaluated in the peritoneal fluid by means of optical microscopy and enzyme immunoassay (ELISA kits), respectively.


RESULTS
The administration of diacerein to febrile animals attenuated Baker's yeast-induced fever but did not alter prostaglandin E(2)-induced fever. Diacerein prevented the development of Baker's yeast-induced fever and significantly attenuated the increase in peritoneal leukocytes and decreased IL-1beta and TNF-alpha levels in peritoneal fluid.


CONCLUSIONS
These data suggest that diacerein partially protects against Baker's yeast-induced fever and peritoneal leukocyte migration, and indicate that this effect appears to be due to inhibition of release of cytokines (such as TNF-alpha and IL-1beta).
Pasin
Juliana Saibt Martins
JS
Department of Physiology, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
Ferreira
Ana Paula Oliveira
AP
Saraiva
André Luis Lopes
AL
Ratzlaff
Viviane
V
Andrighetto
Rosália
R
Tomazetti
Jorgete
J
Avila
Daiana Silva
DS
Alves
Sydney Hartz
SH
Rubin
Maribel Antonello
MA
Ferreira
Juliano
J
Santos
Adair Roberto Soares
AR
Mello
Carlos Fernando
CF
D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001202:Ascitic Fluid / Q000378:metabolism*; D001831:Body Temperature / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D015232:Dinoprostone / Q000009:adverse effects; D004195:Disease Models, Animal; D005334:Fever / Q000139:chemically induced / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000378:metabolism*; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000880:Anthraquinones; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; C025292:diacerein; D015232:Dinoprostone