title: Crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of the human spliceosomal DExD/H-box protein hPrp22.
abstract: The Homo sapiens DExD/H-box protein hPrp22 is a crucial component of the eukaryotic pre-mRNA splicing machinery. Within the splicing cycle, it is involved in the ligation of exons and generation of the lariat and it additionally catalyzes the release of mature mRNA from the spliceosomal U5 snRNP. The yeast homologue of this protein, yPrp22, shows ATP-dependent RNA-helicase activity and is capable of unwinding RNA/RNA duplex molecules. A truncated construct coding for residues 950-1183 of human Prp22, comprising the structurally and functionally uncharacterized C-terminal domain, was cloned into an Escherichia coli expression vector. The protein was subsequently overproduced, purified and crystallized. The crystals obtained diffracted to 2.1 A resolution, belonged to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 78.2, c = 88.4 A, and contained one molecule in the asymmetric unit.
authors.affiliation: Institut für Mikrobiologie und Genetik, Abteilung Molekulare Strukturbiologie, Georg-August-Universität Göttingen, Göttingen, Germany.
authors.forename: Denis
authors.initials: D
authors.lastname: Kudlinzki
authors.forename: Christian
authors.initials: C
authors.lastname: Nagel
authors.forename: Ralf
authors.initials: R
authors.lastname: Ficner
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D053487:DEAD-box RNA Helicases / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D017434:Protein Structure, Tertiary; D000072260:RNA Splicing Factors; D017381:Spliceosomes / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000072260:RNA Splicing Factors; D053487:DEAD-box RNA Helicases; C545297:DHX8 protein, human