Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin.
A new fluorogenic, lanthanide-based oligopeptide substrate for the detection of the zinc-dependent endoprotease thermolysin is described. Using time-resolved fluorescence measurement, a highly sensitive assay for thermolysin was developed with a 50pM detection limit (3.5fmol).
Marguerre
Ann-Kathrin
AK
Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
Krämer
Roland
R
D000595:Amino Acid Sequence; D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013820:Thermolysin / Q000032:analysis*; D015032:Zinc / Q000737:chemistry
D005456:Fluorescent Dyes; D010455:Peptides; D005063:Europium; D013820:Thermolysin; D015032:Zinc