Plasma proteome profiling of von Hippel-Lindau disease after total and subtotal nephrectomy: a preliminary study.
OBJECTIVE
Common treatment of renal cell carcinoma associated with von Hippel-Lindau (VHL) disease is total (bilateral) or subtotal nephrectomy. Whereas total nephrectomy is associated with absolutely no residual renal function, subtotal nephrectomy frequently leads to chronic kidney disease (CKD) with some residual renal functions. However, molecular mechanisms underlying CKD remain unclear and the diagnosis of CKD is frequently accomplished at its late stage.


METHODS
We performed a plasma proteomics study to compare the plasma proteome profile of VHL patient who underwent total nephrectomy to the profiles of VHL patient with subtotal nephrectomy and healthy control. Totally 100 mug proteins from each sample was resolved by two-dimensional electrophoresis (2-DE) in triplicate and visualized with SYPRO Ruby fluorescence stain.


RESULTS
The normal plasma proteome profile markedly differed from the profiles of VHL patients. Comparative analysis between total versus subtotal nephrectomized patients revealed significant differences in levels of 20 plasma proteins. Pathway analysis revealed two important networks involving in lipid metabolism, molecular transport, carbohydrate metabolism, cellular growth and proliferation, and small molecule biochemistry, in which these identified and other proteins interplayed.


CONCLUSIONS
Our data identified potential biomarkers for CKD. Further characterization of these identified proteins might also lead to better understanding of molecular mechanisms underlying CKD.
Opatrná
Sylvie
S
Department of Internal Medicine I, Charles University School of Medicine in Plzen, Plzen, Czech Republic.
Chiangjong
Wararat
W
Korabecná
Marie
M
Sintiprungrat
Kitisak
K
Sinchaikul
Supachok
S
Chen
Shui-Tein
ST
Matejovic
Martin
M
Thongboonkerd
Visith
V
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000473:pathology / Q000601:surgery; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D007680:Kidney Neoplasms / Q000097:blood / Q000473:pathology / Q000601:surgery; D008297:Male; D009392:Nephrectomy; D009942:Organometallic Compounds / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D006623:von Hippel-Lindau Disease / Q000097:blood / Q000473:pathology / Q000601:surgery
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D009942:Organometallic Compounds; D020543:Proteome; C515601:Sypro Ruby