Electric-field-assisted growth of functionalized poly(3,4-ethylenedioxythiophene) nanowires for label-free protein detection.
The construction of functionalized poly(3,4-ethylenedioxythiophene) (PEDOT) nanowire devices for label-free protein detection is reported. Direct growth/assembly of PEDOT nanowires with carboxylic acid side-chain functional groups (poly(EDOT-COOH)) across the electrode junction is achieved by using an electric-field-assisted method. These functionalized PEDOT nanowire devices show typical depletion-mode p-type field-effect transistor (FET) properties. Upon conjugation with a protein-binding aptamer, the PEDOT nanowire FET devices are used for label-free electronic detection of a target protein of interest. The binding of a positively charged protein causes a substantial decrease in current flow, attributed to the specific interaction between target protein molecules and aptamer-conjugated polymer chains.
Xie
Hong
H
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669, Singapore.
Luo
Shyh-Chyang
SC
Yu
Hsiao-Hua
HH
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D046911:Macromolecular Substances / Q000737:chemistry; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D013194:Staining and Labeling; D013499:Surface Properties
D019086:Bridged Bicyclo Compounds, Heterocyclic; D046911:Macromolecular Substances; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene)