title: Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes.
abstract: We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized.
authors.forename: Hieu M
authors.initials: HM
authors.lastname: Dang
authors.forename: Gen
authors.initials: G
authors.lastname: Omura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Umano
authors.forename: Masatomo
authors.initials: M
authors.lastname: Yamagiwa
authors.forename: Shin'ichiro
authors.initials: S
authors.lastname: Kajiyama
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ozeki
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Itoh
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000032:analysis*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014018:Tissue Distribution
chemical_list: D006454:Hemoglobins