Polymorphism of a COLIA1 gene Sp1 binding site in Korean women with pelvic organ prolapse.
OBJECTIVE
To evaluate the possible influence of G-->T substitution at the Sp1-binding site of the COLIA1 gene on the risk of pelvic organ prolapse (POP).


METHODS
The study group consisted of 15 women with advanced stage POP. Fifteen control subjects with uterine myomas among the postmenopausal women were matched for age and parity. DNA was obtained from peripheral blood leukocytes. The fragments of the first intron of the COLIA1 gene were amplified by real time polymerase chain reaction. The polymorphism was identified using LightCycler Technology with hybridization probes. Sequencing reactions were performed on each template using commercial primer.


RESULTS
Two groups had no significant difference in medical history, surgical, and smoking history. The homozygous peaks in two groups were noted at 57 on melting curve analysis. Sequencing reactions confirmed the G/G alleles in the 30 specimens tested. We could not find any polymorphism at the Sp1-binding site in COLIA1 gene with advanced stage POP. Statistical significance was considered to be p < .05.


CONCLUSIONS
The polymorphism of the Sp1-binding site in the COLIA1 gene did not contribute to the development of POP in Korea.
Cho
Hye Jin
HJ
Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.
Jung
Hyun Joo
HJ
Kim
Sei Kwang
SK
Choi
Jong Rak
JR
Cho
Nam Hoon
NH
Bai
Sang Wook
SW
D000368:Aged; D044466:Asian People / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D056887:Pelvic Organ Prolapse / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000378:metabolism*
D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D016329:Sp1 Transcription Factor