title: Miniaturized matrix solid phase dispersion procedure and solid phase microextraction for the analysis of organochlorinated pesticides and polybrominated diphenylethers in biota samples by gas chromatography electron capture detection.
abstract: This work has developed a miniaturized method based on matrix solid phase dispersion (MSPD) using C18 as dispersant and acetonitrile-water as eluting solvent for the analysis of legislated organochlorinated pesticides (OCPs) and polybrominated diphenylethers (PBDEs) in biota samples by GC with electron capture (GC-ECD). The method has compared Florisil-acidic Silica and C18 as dispersant for samples as well as different solvents. Recovery studies showed that the combination of C18-Florisil was better when using low amount of samples (0.1 g) and with low volumes of acetonitrile-water (2.6 mL). The use of SPME for extracting the analytes from the solvent mixture before the injection resulted in detection limits between 0.3 and 7.0 microg kg(-1) (expressed as wet mass). The miniaturized procedure was easier, faster, less time consuming than the conventional procedure and reduces the amounts of sample, dispersant and solvent volume by approximately 10 times. The proposed procedure was applied to analyse several biota samples from different parts of the Comunidad Valenciana.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, C/Dr Moliner, 50, 46100 Burjassot, Valencia, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Moliner-Martinez
authors.forename: P
authors.initials: P
authors.lastname: Campíns-Falcó
authors.forename: C
authors.initials: C
authors.lastname: Molins-Legua
authors.forename: L
authors.initials: L
authors.lastname: Segovia-Martínez
authors.forename: A
authors.initials: A
authors.lastname: Seco-Torrecillas
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002849:Chromatography, Gas / Q000379:methods*; D005504:Food Analysis; D055768:Halogenated Diphenyl Ethers / Q000032:analysis*; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D055768:Halogenated Diphenyl Ethers; D006843:Hydrocarbons, Chlorinated; D010573:Pesticide Residues