Hepal-6 derived RNA electroporated murine spleen B cells induced antitumor effects in vivo.
RNA electroporated CD40 ligand-activated B cells can induce cytotoxic T-lymphocyte response in vitro. In order to evaluate the effects in vivo, we applied murine spleen B cell vaccine in a mouse model of liver cancer. C57BL/6 mouse spleen B cells were activated by anti-mouse CD40 antibody, recombinant interleukin-4, and cyclosporin A, and then electroporated with total RNA from Hepal-6 cells (Hepal-6 RNA-CD40mAb-B cells). This vaccine was injected into C57BL/6 mice that were subcutaneously inoculated with Hepal-6 cells. Hepal-6 RNA-CD40mAb-B cells could induce tumor-specific cytotoxic T cells and IFN-gamma secretion in the immunized mice. In vivo study showed this vaccine could well inhibit tumor progression, improve overall survival, and provide continuous immunoprotection against Hepal-6 cell-induced tumor. Tumor cell-derived total RNA electroporated B cells vaccine is a type of effective immunotherapy and provides potential implication for clinical treatment.
Shen
SuNan
S
Jiangsu Key Laboratory for Molecular Medicine, Medical School of Nanjing University, Nanjing, PR China. shensn@nju.edu.cn
Xu
YanFei
Y
Yu
Feng
F
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D023201:CD40 Ligand / Q000276:immunology / Q000378:metabolism; D019496:Cancer Vaccines / Q000627:therapeutic use*; D018274:Electroporation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012313:RNA / Q000008:administration & dosage*; D013154:Spleen / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
D019496:Cancer Vaccines; D023201:CD40 Ligand; D015847:Interleukin-4; D012313:RNA