title: Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins.
abstract: Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is difficult to detect Babesia organisms by observation of Giemsa-stained thin blood smears. We constructed a monoclonal phage display single chain antibody (scFv) against a B. gibsoni merozoite antigen, P50 protein. Intraerythrocytic B. gibsoni organisms are clearly stained using this antibody. The monoclonal scFv facilitated the detection of B. gibsoni organisms in canine blood samples.
authors.affiliation: Department of Veterinary Parasitological Diseases, Gifu University, Gifu, Japan.
authors.forename: Yoshino
authors.initials: Y
authors.lastname: Hirose
authors.forename: Saeka
authors.initials: S
authors.lastname: Matsuhira
authors.forename: Tatiana Alexandrovna
authors.initials: TA
authors.lastname: Batanova
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Goo
authors.forename: Mohamad A
authors.initials: MA
authors.lastname: Terkawi
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kitoh
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takashima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001403:Babesia / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D001404:Babesiosis / Q000662:veterinary; D017931:DNA Primers; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D017125:Genes, Protozoan / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D013987:Ticks / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; C434466:P50 protein, Babesia gibsoni; D019151:Peptide Library