Determination and pharmacokinetic study of hydrocodone in human plasma by liquid chromatography coupled with tandem mass spectrometry.
A rapid, highly sensitive and specific high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS-MS) quantitation method was developed and validated for the determination of hydrocodone in human plasma. Sample was extracted from 0.5mL heparinized plasma by a simple liquid-liquid extraction method and analyzed on a C18 column with a mobile phase of acetonitrile-water (78:22,v/v,0.1% acetic acid). Detection was carried out by positive elevtrospray ionization (ESI) in multiple reactions monitoring (MRM) mode of 300.3-->199.2 (m/z) for hydrocodone and 341.2-->107.2 (m/z) for canrenone (I.S.), respectively. A good linearity was obtained from 0.5 to 60 ng x mL(-1) and the lower limit of quantification (LLOQ) was 0.1 ng x mL(-1). Compared to an existing method, the extraction method, internal standard and chromatographic conditions were modified and the cost of a large amount of samples determination was decreased obviously. The method was successfully applied to the pharmacokinetic and bioequivalence studies in healthy Chinese volunteers.
Zhang
Rui
R
Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan, China.
Wang
Benjie
B
Wei
Chunmin
C
Yuan
Guiyan
G
Guo
Ruichen
R
D000701:Analgesics, Opioid / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D004355:Drug Stability; D006801:Humans; D006853:Hydrocodone / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D013810:Therapeutic Equivalency; D055815:Young Adult
D000701:Analgesics, Opioid; D006853:Hydrocodone