title: Optimization of the enzymatic production of 20(S)-ginsenoside Rg(3) from white ginseng extract using response surface methodology.
abstract: The optimization of the conversion of ginseng saponin glycosides to 20(S)-ginsenoside Rg(3) by enzymatic transformation was carried out using response surface methodology (RSM) based on a 2(3) factorial central composite design. The production of 20(S)-ginsenoside Rg(3) using several commercial enzymes indicated that the enzyme Cellulase-12T was the most efficient at producing 20(S)-ginsenoside Rg(3). To optimize the enzymatic production of 20(S)-ginsenoside Rg(3), response surface methodology was applied to determine the ideal amount of white ginseng extract, Cellulase-12T and reaction time. These results indicate that white ginseng extract (1.67%) treated with Celluase-12T (3.67%) for 72 hours had 4 times the quantity of 20(S)-ginsenoside Rg(3) compared to commercial white ginseng extract.
authors.affiliation: Division of Animal Life Science, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Chang
authors.forename: Hee Sook
authors.initials: HS
authors.lastname: Jee
authors.forename: Na-Kyoung
authors.initials: NK
authors.lastname: Lee
authors.forename: Se-Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Na-Won
authors.initials: NW
authors.lastname: Lee
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000096:biosynthesis* / Q000737:chemistry; D016018:Least-Squares Analysis; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000378:metabolism*; D012044:Regression Analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
chemical_list: D036145:Ginsenosides; D010936:Plant Extracts; C097367:ginsenoside Rg3; D002480:Cellulase