title: [Mitochondrial DNA D-loop variation types in Tibet mini-pigs in association with the blood parameters].
abstract: OBJECTIVE
  To analyze the mitochondrial DNA (mtDNA) D-loop region sequence variation in Tibet Mini-Pigs in relation to the blood parameters and provide the molecular genetic basis for developing new species of laboratory animals.
  
  
  METHODS
  The genomic DNA was extracted from the whole blood samples of 59 Tibet mini-pigs to amplifying the mtDNA D-loop for sequence analysis. Nine physiological and nine biochemical blood parameters of Tibet mini-pigs were measured .
  
  
  RESULTS
  Based on the variation of the tandem repeat motif, the mtDNA D-loop region of Tibet mini-pigs was classified into two types, namely type A and B with the percentage of 57.6% and 42.4%, respectively, roughly matching the 3 transform sites (305, 500, 691) at the 5' end. In the 18 blood parameters, only red blood cell count showed significant differences between types A and (P<0.01).
  
  
  CONCLUSIONS
  Based on the sequence variation of the mtDNA D-loop region, Tibet mini-pigs can be divided into two types that show a significant difference in red blood cell count.
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou 510515, China. hongtao@zzu.edu.cn
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Li
authors.forename: Qing-hong
authors.initials: QH
authors.lastname: Wu
authors.forename: Jin
authors.initials: J
authors.lastname: Yuan
authors.forename: Dong
authors.initials: D
authors.lastname: Xiao
authors.forename: Wan-shan
authors.initials: WS
authors.lastname: Wang
authors.forename: Jia-ning
authors.initials: JN
authors.lastname: Zhang
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Zhang
authors.forename: Jin-ze
authors.initials: JZ
authors.lastname: Li
authors.forename: Wei-wang
authors.initials: WW
authors.lastname: Gu
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D006403:Hematologic Tests; D016133:Polymerase Chain Reaction; D013552:Swine / Q000097:blood* / Q000235:genetics*; D018609:Tibet
chemical_list: D004272:DNA, Mitochondrial