title: Potentiation of proliferation of some but not all human colon carcinoma cell lines by immobilized hepatic asialoglycoprotein receptor 1.
abstract: Twenty-three human colorectal carcinoma cell lines were examined for the binding of recombinant hepatic asialoglycoprotein receptor 1 (ASGR1), which is known to be exclusively expressed on hepatic parenchymal cells. The effects of the binding were assessed by adhesion to and proliferation on immobilized recombinant ASGR1. Recombinant ASGR1 bound strongly to six cell lines and moderately to 15 cell lines out of 23 lines tested, as shown by flow cytometric analysis. The first six cell lines (group A) also exhibited strong adherence to immobilized ASGR1, whereas 11 of the 15 cell lines of the second group (group B) showed significant adhesion with smaller enhancement by ASGR1 than the cell lines in group A. With a representative cell line (DLD-1 cells categorized in group B), a significant portion of the adhesion was inhibited by preincubation of ASGR1 with asialofetuin, a competitive inhibitor of the carbohydrate recognition by ASGR1. The growth rates of 13 cell lines (two of group A and 11 of group B) were significantly accelerated when they were cultured on immobilized recombinant ASGR1. The results indicate that ASGR is a potential organ-specific microenvironmental factor for colorectal carcinoma growth and metastasis formation in livers.
authors.affiliation: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Fang
authors.forename: Ryota
authors.initials: R
authors.lastname: Izawa
authors.forename: Laura
authors.initials: L
authors.lastname: Gomez-Santos
authors.forename: Suguru
authors.initials: S
authors.lastname: Ueno
authors.forename: Tomoya
authors.initials: T
authors.lastname: Sawaguchi
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Usami
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nodera
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ohashi
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Higashi
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Irimura
mesh_terms: D037263:Asialoglycoprotein Receptor / Q000378:metabolism*; D001212:Asialoglycoproteins / Q000494:pharmacology; D002448:Cell Adhesion; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D060748:Fetuins; D005434:Flow Cytometry; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000494:pharmacology
chemical_list: D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D060748:Fetuins; D011994:Recombinant Proteins; D000509:alpha-Fetoproteins; C017029:asialofetuin