title: Multimarker approach analysis in common carp Cyprinus carpio sampled from three freshwater sites.
abstract: The aim of this study is to assess the response of a multimarker approach in common carp Cyprinus carpio sampled from three Tunisian dam lakes selected according to different environmental and ecological characteristics. Glutathione-S-transferase (GST) activity was analyzed in carp liver and used as a phase II detoxification enzyme, hepatic metallothionein content (MTs) was used as a metallic stress indicator, and cholinesterase activities were analyzed in muscle and brain and used as neurotoxicity biomarker. Micronucleus frequency test (MN) as a genotoxicity marker. GST and MT levels showed an increase in fish from the Bir Mcherga site and a decrease in Sidi Saâd site with respect to fish from Nebhana site. Results showed a strong inhibition of cholinesterase activities in fish from Bir Mcherga and Sidi Saâd sites compared to Nebhana site. Relatively high level of MN is reported specially in fish blood from the Bir Mcherga site.
authors.affiliation: Laboratoire de Biochimie et de Toxicologie Environnementale, Institut Supérieur Agronomique de Chott-Mariem, Sousse, Tunisia.
authors.forename: Sofiene
authors.initials: S
authors.lastname: Tlili
authors.forename: Jamel
authors.initials: J
authors.lastname: Jebali
authors.forename: Mohamed
authors.initials: M
authors.lastname: Banni
authors.forename: Zohra
authors.initials: Z
authors.lastname: Haouas
authors.forename: Ammar
authors.initials: A
authors.lastname: Mlayah
authors.forename: Ahmed Noureddine
authors.initials: AN
authors.lastname: Helal
authors.forename: Hamadi
authors.initials: H
authors.lastname: Boussetta
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D002347:Carps / Q000378:metabolism*; D002802:Cholinesterases / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D050217:Metabolic Detoxication, Phase II; D008668:Metallothionein / Q000378:metabolism; D015162:Micronucleus Tests; D009132:Muscles / Q000201:enzymology / Q000378:metabolism; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity; D014416:Tunisia; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D015415:Biomarkers; D009153:Mutagens; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D005982:Glutathione Transferase; D002802:Cholinesterases