Oxidative stress response to menadione and cumene hydroperoxide in the opportunistic fungal pathogen Candida glabrata.
Candida glabrata is an opportunistic fungal pathogen that can cause severe invasive infections and can evade phagocytic cell clearance. We are interested in understanding the virulence of this fungal pathogen, in particular its oxidative stress response. Here we investigated C. glabrata, Saccharomyces cerevisiae and Candida albicans responses to two different oxidants: menadione and cumene hydroperoxide (CHP). In log-phase, in the presence of menadione, C. glabrata requires Cta1p (catalase), while in a stationary phase (SP), Cta1p is dispensable. In addition, C. glabrata is less resistant to menadione than C. albicans in SP. The S. cerevisiae laboratory reference strain is less resistant to menadione than C. glabrata and C. albicans; however S. cerevisiaeclinical isolates (CIs) are more resistant than the lab reference strain. Furthermore, S. cerevisiae CIs showed an increased catalase activity. Interestingly, in SP C. glabrata and S. cerevisiae are more resistant to CHP than C. albicans and Cta1p plays no apparent role in detoxifying this oxidant.
Cuéllar-Cruz
Mayra
M
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí 78216 , México.
Castaño
Irene
I
Arroyo-Helguera
Omar
O
De Las Peñas
Alejandro
A
D001555:Benzene Derivatives / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism; D041221:Candida glabrata / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D024483:Vitamin K 3 / Q000494:pharmacology*
D001555:Benzene Derivatives; D016877:Oxidants; D024483:Vitamin K 3; D002374:Catalase; C007164:cumene hydroperoxide