[Modern aspects of diagnostics of chronic chlamydiosis caused by persisting forms of Chlamydia].
OBJECTIVE
To study the possible hematogenic route of dissemination of Chlamydia trachomatis and to analyze efficacy of methods of pathogen detection in clinical specimens (sera and scraping material).


METHODS
Cultural method, electron microscopy, real-time PCR, immunofluorescent assay.


RESULTS
C. trachomatis was detected in blood by using 2 tests (culture and PCR) in 95.2% of patients with confirmed Chlamydia infection. Chlamydia isolated from blood had infectious properties that could point to the presence of weakly studied hematogenic route of dissemination of C. trachomatis in host's organism. Study of diagnostic value of pathogen detection in serum showed that in case of chronic diseases of urogenital tract as well as extragenital diseases, rate of C. trachomatis detection in serum was significantly higher (61.1% of cases compared to 16.7% in scraping material).


CONCLUSIONS
It is the first time when data about possible circulation of C. trachomatis in blood of patients was obtained. Detection of C. trachomatis in serum of patients with chronic and complicated forms of chlamydiosis provides essentially new approach for direct identification of the pathogen irrespectively from localization of infection's locus.
Pashko
Iu P
IuP
Zigangirova
N A
NA
Petiaev
Iu M
IuM
Kapotina
L N
LN
Morgunova
E Iu
EIu
Kolkova
N I
NI
Didenko
L V
LV
Iudina
T I
TI
D000818:Animals; D016470:Bacteremia / Q000175:diagnosis; D002460:Cell Line; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology; D051379:Mice; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D014521:Urethra / Q000382:microbiology
D004269:DNA, Bacterial