title: Jadassohn-Pellizzari anetoderma: study of multiphoton microscopy based on two-photon excited fluorescence and second harmonic generation.
abstract: Anetoderma is a rare skin disease with loss of dermal elastic tissue resulting in clinically localized areas of flaccid or herniated sack-like skin. In this study, we report a case of Jadassohn-Pellizzari anetoderma, in a 21-year-old Chinese female with an 18-year history of progressively generalized wrinkled skin lesions. Multiphoton microscopy based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) was firstly employed to investigate the pathological process from unaffected skin to the erythematous phase and finally with affected skin of this case. The results showed that the normal elastic fibers in unaffected skin were almost completely absent in erythematous skin tissue, then replaced by a lot of elastic fibers with granular morphology in affected skin, which was consistent with the histopathological results. The obvious changes in collagen fibers and the occurrence of inflammatory cell infiltration in erythematous tissue suggested that the variations of these two components were also the main pathogenesis of anetoderma, except for the deficiency of elastic fibers. Based on these data, we demonstrated that multiphoton microscopy was a promising tool for non-invasive investigation of the pathology of anetoderma at nearly histological resolution, and has potential for observing the dermatological dynamic processes for living specimens because it is based on the intrinsic signals of tissue components.
authors.affiliation: Department of Dermatology, Affiliated Union Hospital Fujian Medical University, Fuzhou 350001, China. zhaomyco@163.com
authors.forename: Jingjun
authors.initials: J
authors.lastname: Zhao
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Yang
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.forename: Xingshan
authors.initials: X
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.forename: Xiaoyin
authors.initials: X
authors.lastname: Ye
authors.forename: Lihang
authors.initials: L
authors.lastname: Lin
authors.forename: Shusen
authors.initials: S
authors.lastname: Xie
mesh_terms: D000328:Adult; D057088:Anetoderma / Q000175:diagnosis / Q000473:pathology*; D003287:Contrast Media / Q000737:chemistry; D004547:Elastic Tissue / Q000473:pathology*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted; D036641:Microscopy, Fluorescence, Multiphoton; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015595:Skin Aging / Q000473:pathology*
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes