Influence of ethanol on the release of growth factors in human blood-derived platelet gels.
Platelet gels (PG) are new topical single-donor blood products which are attracting great interest in regenerative medicine. They are obtained by mixing a platelet-rich plasma fraction with thrombin to generate a fibrin gel enriched in platelet growth factors (GF). The type of thrombin preparation may affect PG reproducibility. We have determined the impact of 14.6% (v/v) ethanol-stabilized thrombin (EHT) on the release of GF by platelets. Various ratios of EHT and platelet concentrates were mixed to obtain from 2.43 to 7.96% ethanol concentration. Platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1), vascular endothelium growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were assessed at 5, 120, and 300 min after PG formation. Protein profiles of thrombin and PG releasates were analyzed by SDS-PAGE. The amount of PDGF-AB, TGF-beta1, and VEGF released per platelet decreased significantly (p<0.05) with increasing ethanol concentrations but, however, not that of EGF. IGF-1 content was stable, consistent with its presence mostly in plasma. SDS-PAGE indicated that ethanol did not affect fibrin formation. In conclusion, ethanol has a significant impact on the amount of GF released by platelets and should be strictly controlled to standardize PG and optimize clinical benefits.
Kuo
Ya Po
YP
Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
Lee
Yueh-Lun
YL
Tseng
Yu Hong
YH
Su
Ching-Hua
CH
Burnouf
Thierry
T
Su
Chen Yao
CY
D000465:Algorithms; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001772:Blood Cell Count; D001781:Blood Component Removal / Q000379:methods; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology*; D005337:Fibrin / Q000378:metabolism; D005782:Gels; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D013917:Thrombin / Q000378:metabolism
D000891:Anti-Infective Agents, Local; D005782:Gels; D036341:Intercellular Signaling Peptides and Proteins; D012997:Solvents; D000431:Ethanol; D005337:Fibrin; D013917:Thrombin