title: Experimental assessment of autologous lymph node transplantation as treatment of postsurgical lymphedema.
abstract: BACKGROUND
  The authors' objective was to test whether the transplantation of an autologous lymph node into a nodal excision site in sheep would restore lymphatic transport function and reduce the magnitude of postsurgical lymphedema.
  
  
  METHODS
  As a measure of lymph transport, iodine-125 human serum albumin was injected into prenodal vessels at 8 and 12 weeks after surgery, and plasma levels of the protein were used to calculate the transport rate of the tracer to blood (percent injected per hour). Edema was quantified from the circumferential measurement of the hind limbs.
  
  
  RESULTS
  The transplantation of avascular lymph nodes at 8 (n = 6) and 12 weeks (n = 6) produced lymphatic function levels of 12.3 +/- 0.5 and 12.6 +/- 0.8, respectively. These values were significantly less (p < 0.001) than those measured at similar times in the animals receiving sham surgical procedures (16.6 +/- 0.7, n = 6; and 16.1 +/- 0.7, n = 6, respectively). When vascularized transplants were performed, lymphatic function was similar to the sham controls and significantly greater (p < 0.001) than that of the avascular group (8 weeks, 15.8 +/- 0.9, n = 8; 12 weeks, 15.7 +/- 1.0, n = 10). Lymph transport correlated significantly with the health of the transplanted nodes (scaled with histologic analysis) (p < 0.0001). The vascularized node transplants (n = 18) were associated with the greatest clinical improvement, with the magnitude of edema in these limbs exhibiting significantly lower levels of edema (p = 0.039) than nontreated limbs (n = 18).
  
  
  CONCLUSIONS
  The successful reimplantation of a lymph node into a nodal excision site has the potential to restore lymphatic function and facilitate edema resolution. This result has important conceptual implications in the treatment of postsurgical lymphedema.
authors.affiliation: Toronto, Ontario, Canada From the Brain Sciences Program, Department of Laboratory Medicine and Pathobiology, and Molecular and Cell Biology, Department of Medical Biophysics, Sunnybrook Health Sciences Center, and the Department of Surgery, Women's College Hospital, University of Toronto.
authors.forename: Dalia
authors.initials: D
authors.lastname: Tobbia
authors.forename: John
authors.initials: J
authors.lastname: Semple
authors.forename: Amy
authors.initials: A
authors.lastname: Baker
authors.forename: Dan
authors.initials: D
authors.lastname: Dumont
authors.forename: Miles
authors.initials: M
authors.lastname: Johnston
mesh_terms: D000818:Animals; D005260:Female; D005471:Fluoroscopy; D006614:Hindlimb; D008196:Lymph / Q000502:physiology; D008197:Lymph Node Excision / Q000009:adverse effects*; D008198:Lymph Nodes / Q000166:cytology / Q000000981:diagnostic imaging / Q000637:transplantation*; D008209:Lymphedema / Q000209:etiology / Q000601:surgery*; D008297:Male; D008866:Microsurgery; D012756:Sheep; D014182:Transplantation, Autologous