title: Purification, crystallization and initial X-ray diffraction study of the zinc-finger domain of zebrafish Nanos.
abstract: Nanos is a highly conserved RNA-binding protein in higher eukaryotes and acts as a key regulator protein involved in translational control utilizing the 3' untranslated region of mRNA. The C-terminal domain of Nanos has two conserved and novel CCHC-type zinc-finger motifs that are responsible for the function of Nanos. To clarify the structural basis of the function of Nanos, the C-terminal domain (residues 59-159) of zebrafish Nanos was overexpressed, purified and crystallized. The crystal belonged to space group P6(3), with unit-cell parameters a = b = 100.9, c = 71.5 A, gamma = 120 degrees. Structure determination by the MAD/SAD method is now in progress.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, Tsurumi, Yokohama, Kanagawa, Japan. hash@tsurumi.yokohama-cu.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Shigeta
authors.initials: S
authors.lastname: Kawaguchi
authors.forename: Kodai
authors.initials: K
authors.lastname: Hara
authors.forename: Keishi
authors.initials: K
authors.lastname: Nakamura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins; D016415:Sequence Alignment; D014961:X-Ray Diffraction; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D016335:Zinc Fingers
chemical_list: D016601:RNA-Binding Proteins; D029961:Zebrafish Proteins; C440015:nanos3 protein, zebrafish