title: Kinetics of solvent-free lipase-catalyzed glycerolysis of olive oil in surfactant system.
abstract: This work reports experimental data and kinetic modeling of solvent-free glycerolysis of olive oil using a commercial immobilized lipase (Novozym 435) in the presence of Triton X-100 surfactant for the production of monoacylglycerols (MAG) and diacylglycerols (DAG). The experiments were performed in batch mode evaluating the effects of temperature (30-70 degrees C), enzyme concentration (2.5-18 wt %), Triton X-100 concentration (10-20 wt %), and glycerol to oil molar ratio (3:1, 6:1, and 9:1). Experimental results showed that lipase-catalyzed solvent-free glycerolysis with the addition of Triton X-100 might be a potential alternative route to conventional organic solvent methods, as good conversions were obtained with relatively low enzyme concentrations (9 wt %) in short reaction times (240 min). The glycerolysis and hydrolysis parallel reactions were considered with rate constants estimated by minimizing a maximum likelihood function. A very satisfactory agreement between experimental data and model results was obtained, thus allowing a better understanding of the reaction kinetics.
authors.affiliation: Department of Food Engineering, URI - Campus de Erechim, Av. Sete de Setembro 1621, Erechim, 99700-000 RS, Brazil.
authors.forename: Alexsandra
authors.initials: A
authors.lastname: Valério
authors.forename: Roberta L
authors.initials: RL
authors.lastname: Krüger
authors.forename: Jorge
authors.initials: J
authors.lastname: Ninow
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Corazza
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
authors.forename: Marcos L
authors.initials: ML
authors.lastname: Corazza
mesh_terms: D004075:Diglycerides / Q000378:metabolism; D004800:Enzymes, Immobilized; D005990:Glycerol / Q000032:analysis / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000378:metabolism; D017830:Octoxynol / Q000008:administration & dosage; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry*; D013501:Surface-Active Agents / Q000008:administration & dosage
chemical_list: D004075:Diglycerides; D004800:Enzymes, Immobilized; D050178:Monoglycerides; D000069463:Olive Oil; D010938:Plant Oils; D013501:Surface-Active Agents; D017830:Octoxynol; D008049:Lipase; D005990:Glycerol