title: Simultaneous quantitative analysis of Fc gamma RI (CD64) and CR1 (CD35) on neutrophils in distinguishing between bacterial infections, viral infections, and inflammatory diseases.
abstract: A flow cytometric quantitative analysis of receptors on neutrophils can be exploited in distinguishing between inflammatory and infectious diseases. In this prospective comparative study, simultaneous quantitative analysis of CD64 and CD35 on peripheral blood neutrophils was performed in febrile patients in order to differentiate between bacterial infections (n=89), viral infections (n=46), and inflammatory diseases (n=21). The patient data was compared to 60 healthy controls. We could divide patients into three groups depending on how they express CD35 and CD64 on neutrophils: (1) patients with a high probability of viral infection (low CD35/low CD64 and low CD35/high CD64), (2) patients with a high probability of inflammatory disease (high CD35/low CD64), and (3) patients with a high probability of bacterial infection (high CD35/high CD64). In summary, simultaneous quantitative analysis of CD64 and CD35 on neutrophils could potentially assist physicians to distinguish between inflammatory and infectious diseases.
authors.affiliation: Department of Medicine, Turku University Central Hospital, Turku, Finland.
authors.forename: Päivi
authors.initials: P
authors.lastname: Jalava-Karvinen
authors.forename: Ulla
authors.initials: U
authors.lastname: Hohenthal
authors.forename: Iina
authors.initials: I
authors.lastname: Laitinen
authors.forename: Pirkko
authors.initials: P
authors.lastname: Kotilainen
authors.forename: Allan
authors.initials: A
authors.lastname: Rajamäki
authors.forename: Jukka
authors.initials: J
authors.lastname: Nikoskelainen
authors.forename: Esa-Matti
authors.initials: EM
authors.lastname: Lilius
authors.forename: Jari
authors.initials: J
authors.lastname: Nuutila
mesh_terms: D000328:Adult; D001424:Bacterial Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D005334:Fever / Q000276:immunology / Q000382:microbiology / Q000821:virology; D005434:Flow Cytometry; D006801:Humans; D007154:Immune System Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D011446:Prospective Studies; D017463:Receptors, Complement 3b / Q000097:blood* / Q000276:immunology; D017452:Receptors, IgG / Q000097:blood* / Q000276:immunology; D018709:Statistics, Nonparametric; D014777:Virus Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology*
chemical_list: C509991:CR1 protein, human; D017463:Receptors, Complement 3b; D017452:Receptors, IgG; D002097:C-Reactive Protein