Immunoassay for cadmium detection and quantification.
OBJECTIVE
To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES).


METHODS
An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated.


RESULTS
IC-ELISA showed an IC50 of 45.6 microg/L with a detection limit of 1.95 microg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998.


CONCLUSIONS
IC-ELISA can detect and quantify cadmium residue in environmental or food samples.
Liu
Gong-Liang
GL
School of Biosciences & Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
Wang
Ju-Fang
JF
Li
Zhi-Yong
ZY
Liang
Shi-Zhong
SZ
Wang
Xiao-Ning
XN
D000818:Animals; D000911:Antibodies, Monoclonal; D002104:Cadmium / Q000737:chemistry*; D004785:Environmental Pollutants / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D004785:Environmental Pollutants; D002104:Cadmium