title: Pseudoclavibacter chungangensis sp. nov., isolated from activated sludge.
abstract: The taxonomic position of a Gram-positive, non-spore-forming strain, designated CAU 59(T), from activated sludge was investigated. Colony morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 59(T) possessed the characteristics of the genus Pseudoclavibacter. Comparative 16S rRNA gene sequence analysis showed sequence divergence values between strain CAU 59(T) and other described Pseudoclavibacter species of more than 3.6 %, and the strain formed a hitherto-unknown subline within the genus Pseudoclavibacter. DNA-DNA hybridization studies showed that strain CAU 59(T) displayed 20.9 % relatedness to its closest phylogenetic neighbour, Pseudoclavibacter helvolus DSM 20419(T). The DNA G+C content was 66.2 mol%. The phenotypic, chemotaxonomic and genotypic data indicated that strain CAU 59(T) represents a novel species of the genus Pseudoclavibacter, for which the name Pseudoclavibacter chungangensis sp. nov. is proposed. The type strain is CAU 59(T) (=KCTC 22691(T) =CCUG 58142(T)).
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
authors.forename: Sung-Lim
authors.initials: SL
authors.lastname: Cho
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.|Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Jung
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
authors.forename: Mi-Hak
authors.initials: MH
authors.lastname: Park
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Young-Hyo
authors.initials: YH
authors.lastname: Chang
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Department of Urology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
authors.forename: Soon Chul
authors.initials: SC
authors.lastname: Myung
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D001483:Base Sequence; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage