title: Validation of intervention strategies to control Escherichia coli O157:H7 and Salmonella typhimurium DT 104 in mechanically tenderized and brine-enhanced beef.
abstract: After three different outbreaks were linked to the consumption of nonintact meat products contaminated with Escherichia coli O157:H7, the U.S. Department of Agriculture, Food Safety and Inspection Service published notice requiring establishments producing mechanically tenderized and moisture-enhanced beef products to reassess their respective hazard analysis and critical control point system, due to potential risk to the consumers. The purpose of this study was to determine the effectiveness of different intervention strategies (lactic acid, lactic acid bacteria, and acidified sodium chlorite) to control E. coli O157:H7 and Salmonella enterica serotype Typhimurium definitive phage type 104 in mechanically tenderized and brine-enhanced beef strip loins when applied to the steaks prior to packaging and shipment for processing. After the mechanical process, translocation of E. coli O157:H7 and Salmonella Typhimurium DT 104 from the surface into the internal muscles occurred at levels between 2.0 and 4.0 log CFU/g (from an initial inoculation level of 5.0 log) after mechanical tenderization, and at levels of 1.0 to 3.0 log CFU/g after injection, with all the interventions consistently presenting lower microbial counts (P < 0.05) than did the controls. Lactic acid bacteria reduced internal E. coli O157:H7 loads 1.2 to > 2.2 log cycles, while the acidified sodium chlorite and lactic acid reduced them between 0.8 and 3.0 log, respectively. Salmonella Typhimurium DT 104 was also reduced internally after application of all interventions between 0.9 and 2.2 log. The application of antimicrobials to the steaks prior to packaging and shipment on day 0 was effective in reducing internalization of both pathogens in nonintact beef products stored for both 14 and 21 days.
authors.affiliation: Department of Animal and Food Sciences, Texas Tech University, Box 42141, Lubbock, Texas 79409, USA.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Echeverry
authors.forename: J Chance
authors.initials: JC
authors.lastname: Brooks
authors.forename: Markus F
authors.initials: MF
authors.lastname: Miller
authors.forename: Jesse A
authors.initials: JA
authors.lastname: Collins
authors.forename: Guy H
authors.initials: GH
authors.lastname: Loneragan
authors.forename: Mindy M
authors.initials: MM
authors.lastname: Brashears
mesh_terms: D000818:Animals; D000898:Antibiosis; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development*; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods / Q000592:standards*; D005516:Food Microbiology; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology; D007777:Lactobacillaceae / Q000502:physiology; D008461:Meat Products / Q000382:microbiology*; D018570:Risk Assessment; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development*; D013997:Time Factors
chemical_list: D002712:Chlorides; D004202:Disinfectants; D019344:Lactic Acid; C001599:chlorite