title: Immunogenicity of bacteriophage lambda particles displaying porcine Circovirus 2 (PCV2) capsid protein epitopes.
abstract: Phage lambda particles displaying four immunodominant regions of porcine Circovirus 2 (PCV2) capsid protein (LDP-D-CAP) was shown to be immunogenic in pigs. The immunodominant regions were fused to the carboxyl-terminal of lambda head protein D. Expression of D-CAP on lambda display particles was demonstrated by ELISA and Western blots. Pigs receiving LDP-D-CAP, without incorporating adjuvant, showed significant anti-PCV2 immune response following the primary vaccination. The LDP-D-CAP preparation induced PCV2 neutralizing antibodies. Delayed type hypersensitivity (DTH) reaction scores revealed that the immunized pigs were hypersensitive to both lambda phage and PCV2 antigens. The LDP-D-CAP elicited both cellular and humoral immune responses. Neither LDP-D-CAP nor the lambda control elicited any untoward local or systemic reactions following immunization. These studies produced the first potential phage vaccine to porcine Circovirus 2.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Canada.
authors.forename: Lakshman N A
authors.initials: LN
authors.lastname: Gamage
authors.forename: John
authors.initials: J
authors.lastname: Ellis
authors.forename: Sidney
authors.initials: S
authors.lastname: Hayes
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D010582:Bacteriophage lambda / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D017925:Circovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000821:virology; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D009500:Neutralization Tests; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000276:immunology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D011994:Recombinant Proteins; D014765:Viral Vaccines