Surfactant enhanced ricinoleic acid production using Candida rugosa lipase.
In this study, ricinoleic acid was produced on surfactant enhanced castor oil hydrolysis using Candida rugosa lipase. The most effective surfactant was Span 80. Employing fractional factorial design, the most suitable temperature and surfactant concentration were found to be 31 degrees C and 0.257% (w/w in buffer) respectively whereas pH, enzyme concentration, buffer concentration and agitation were identified as the most significant independent variables. A 2(4) full factorial central composite design was applied and the optimal conditions were found to be pH 7.0, enzyme concentration 7.42 mg/g oil, buffer concentration 0.20 g/g oil and agitation 1400 rpm with the maximum response of 76% in 4 h. The most important variable was pH, whereas enzyme and buffer concentrations also showed pronounced effect on response. This is the first report on the application of response surface methodology for optimizing surfactant enhanced ricinoleic acid production using C. rugosa lipase.
Goswami
Debajyoti
D
Department of Chemical Engineering, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. dgoswami2@rediffmail.com
Sen
Ramkrishna
R
Basu
Jayanta Kumar
JK
De
Sirshendu
S
D002175:Candida / Q000145:classification* / Q000201:enzymology*; D002368:Castor Oil / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques; D004789:Enzyme Activation; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry*; D012277:Ricinoleic Acids / Q000138:chemical synthesis*; D013501:Surface-Active Agents / Q000737:chemistry*
D012277:Ricinoleic Acids; D013501:Surface-Active Agents; D002368:Castor Oil; D008049:Lipase; C030521:ricinoleic acid