A novel, generic, electroanalytical immunoassay format utilising silver nano-particles as a bio-label.
The aim of this study was to evaluate a novel, generic, sensitive electroanalytical platform method for binding reactions, such as immuno or DNA assays. It was thought that silver nano-particles of 40 nM when attached to the analyte of interest would give an electroanalytical amplification of approx. 10(6) resulting from silver ions produced by dissolution of the nano-particles by a mild chemical oxidant such as ferricyanide. Ferricyanide has been widely used in biochemical measurement and has been shown to relatively stable in a biosensor device. Here we have demonstrated the use of silver nano-particles as a bio-conjugate in a 96 well sandwich assay format for measuring human chorionic gonadotropin (hCG) to a concentration of 0.2 mIU.
Porter
Robert
R
National Physical Laboratory, Teddington, Middlesex, UK. robert.porter@npl.co.uk
Kabil
Alena
A
Forstern
Camilla
C
Slevin
Christopher
C
Kouwenberg
Katherine
K
Szymanski
Mateusz
M
Birch
Brian
B
D006063:Chorionic Gonadotropin / Q000032:analysis*; D005292:Ferricyanides / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry*; D011199:Potentiometry / Q000379:methods*; D012834:Silver / Q000737:chemistry*
D006063:Chorionic Gonadotropin; D005292:Ferricyanides; D012834:Silver