[The effect of IL-18 on E-cadherin expression and its possible intracellular pathway in HK-2 cells].
OBJECTIVE
To explore the effect of NF-kappaB signaling pathway in interleukin-18-induced transdifferentiation in renal proximal tubular cells.


METHODS
In IL-18 stimulation groups, HK-2 cells were challenged with different concentrations (0, 1, 10, 100 microg/L) of IL-18 for 72 h; In SN50 preincubation groups, HK-2 cells were incubated with 100 mg/L SN50 for 30 min before IL-18 was added. At the end of the incubation, the expression of E-cadherin was determined by the combination of immunocytochemistry and RT-PCR.


RESULTS
Percentage of E-cadherin positive expression HK-2 cells was decreased by IL-18 in dosage dependant manner. Expression of E-cadherin mRNA in HK-2 cells was also decreased by IL-18 in dosage dependant manner. Recovered the mostly part of expression of E-cadherin was found after HK-2 cells were pretreated with SN50.


CONCLUSIONS
IL-18-induced transdifferentiation of RTECs is suppressed by blocking NF-kappaB signaling pathway. IL-18-induced transdifferentiation of RTECs at least is partly via NF-kappaB pathway.
Chen
Wei
W
Institute of Nephrologic, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
Liang
Dong
D
Yao
Cui-wei
CW
Tao
Jing-li
JL
Chen
Xiao-wen
XW
Liu
Hua-feng
HF
D015820:Cadherins / Q000235:genetics*; D002478:Cells, Cultured; D006801:Humans; D020382:Interleukin-18 / Q000494:pharmacology*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects*
D015820:Cadherins; D020382:Interleukin-18; D016328:NF-kappa B; D010455:Peptides; D012333:RNA, Messenger; C118258:SN50 peptide