title: Cobalt toxicity: chemical and radiological combined effects on HaCaT keratinocyte cell line.
abstract: Cobalt (Co) is an essential trace element well known as a constituent of vitamin B(12), but different compounds of Co are also described as highly toxic and/or radiotoxic for individuals or the environment. In nuclear power plants, (58)Co and (60)Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl(2)) with or without gamma-ray doses to mimic contamination by (60)Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate gamma-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species.
authors.affiliation: CEA Fontenay aux Roses, DSV/IRCM/SCSR/LRTS, Fontenay aux Rose, France.
authors.forename: N
authors.initials: N
authors.lastname: Gault
authors.forename: C
authors.initials: C
authors.lastname: Sandre
authors.forename: J-L
authors.initials: JL
authors.lastname: Poncy
authors.forename: C
authors.initials: C
authors.lastname: Moulin
authors.forename: J-L
authors.initials: JL
authors.lastname: Lefaix
authors.forename: C
authors.initials: C
authors.lastname: Bresson
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002999:Clone Cells; D003035:Cobalt / Q000378:metabolism / Q000633:toxicity*; D003037:Cobalt Radioisotopes / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000528:radiation effects; D018570:Risk Assessment; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects
chemical_list: D003037:Cobalt Radioisotopes; D017382:Reactive Oxygen Species; D003035:Cobalt; C018021:cobaltous chloride