Enantioselective binding of L,D-phenylalanine to ct DNA.
The enantioselective binding of L,D-phenylalanine to calf thymus DNA was studied by absorption, circular dichroism, fluorescence quenching, viscosity, salt effect and emission experiments. The results obtained from absorption, circular dichroism, fluorescence quenching and viscosity experiments excluded the intercalative binding and salt effect experiments did not support electrostatic binding. So the binding of l,d-phenylalanine to ct DNA should be groove binding. Furthermore, the emission spectra revealed that the binding is enantioselective.
Zhang
Lijin
L
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China.
Xu
Jianhua
J
Huang
Yan
Y
Min
Shungeng
S
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D005453:Fluorescence; D009690:Nucleic Acid Conformation; D010649:Phenylalanine / Q000032:analysis / Q000378:metabolism*; D012965:Sodium Chloride / Q000737:chemistry; D013053:Spectrophotometry; D014783:Viscosity
D012965:Sodium Chloride; D010649:Phenylalanine; D004247:DNA; C427203:calf thymus DNA