title: Photoactive diazoketo-functionalized self-assembled monolayer for biomolecular patterning.
abstract: A diazoketo-functionalized alkoxysilane compound was synthesized, and its self-assembled monolayer (SAM) formation was studied on glass and silicon substrates. Infrared spectroscopy was employed to follow the photoreaction in this system, which proved that carboxylic groups were generated from diazoketo groups in the surface of the substrate. Photopatterning of biotin/streptavidin on the diazoketo-functionalized SAM shows the potential of this novel platform for biomolecular patterning.
authors.affiliation: Department of Chemistry and School of Molecular Science (BK21), Korea Advanced Institute of Science and Technology (KAIST), 373-1, Guseong-Dong, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Ganesan
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jin-Baek
authors.initials: JB
authors.lastname: Kim
mesh_terms: D000090:Acetoacetates / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D007659:Ketones / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010777:Photochemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000090:Acetoacetates; D001391:Azo Compounds; D007659:Ketones; C024840:ethyl acetoacetate; D012825:Silicon