Development of immunochromatographic assay for the on-site detection of salbutamol.
Salbutamol, one of the beta-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80 ng/mL of salbutamol in PBS. The method can be useful as an "on-site" screening procedure for detection of salbutamol.
Khamta
Yaowapa
Y
Division of Clinical Immunology, Faculty of Associated Medical Sciences, Chiang Mai University, Thailand.
Pattarawarapan
Mookda
M
Nangola
Sawitree
S
Tayapiwatana
Chatchai
C
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000420:Albuterol / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis; D003429:Cross Reactions / Q000276:immunology; D017979:Gold Colloid / Q000737:chemistry; D007118:Immunoassay; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology
D000318:Adrenergic beta-Agonists; D000906:Antibodies; D017979:Gold Colloid; D012710:Serum Albumin, Bovine; D002701:Chloramphenicol; D000420:Albuterol; D002976:Clenbuterol