title: Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers.
abstract: Cancer stem cells are known for their inherent resistance to therapy. Here we investigated whether normal stem cells with acquired resistance to stress can be used to identify novel markers of cancer stem cells. For this, we generated a human embryonic stem cell line resistant to Trichostatin A and analyzed changes in its gene expression. The resistant cells over-expressed various genes associated with tumor aggressiveness, many of which are also expressed in the CD133+ glioma cancer stem cells. These findings suggest that stress-resistant stem cells generated in vitro may be useful for the discovery of novel markers of cancer stem cells.
authors.affiliation: The Pharmaceutical Research Institute at Albany, Albany College of Pharmacy and Health Sciences, Albany, NY, USA.
authors.forename: Shaker A
authors.initials: SA
authors.lastname: Mousa
authors.forename: Thangirala
authors.initials: T
authors.lastname: Sudha
authors.forename: Evgeny
authors.initials: E
authors.lastname: Dyskin
authors.forename: Usawadee
authors.initials: U
authors.lastname: Dier
authors.forename: Christine
authors.initials: C
authors.lastname: Gallati
authors.forename: Christine
authors.initials: C
authors.lastname: Hanko
authors.forename: Sridar V
authors.initials: SV
authors.lastname: Chittur
authors.forename: Abdelhadi
authors.initials: A
authors.lastname: Rebbaa
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics*; D015153:Blotting, Western; D002454:Cell Differentiation; D002478:Cells, Cultured; D019008:Drug Resistance, Neoplasm; D053595:Embryonic Stem Cells / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D006877:Hydroxamic Acids; C000606023:PROM1 protein, human; D010455:Peptides; D012333:RNA, Messenger; C012589:trichostatin A