title: Control of Listeria monocytogenes by lauric arginate on frankfurters formulated with or without lactate/diacetate.
abstract: Listeria monocytogenes (Lm) is a food safety concern that can be associated with ready-to-eat (RTE) meat and poultry products because of its persistence in the processing environment. Listeriosis has a fatality rate of 28% in immuno-compromised individuals. RTE meats receive a lethal heat treatment but may become contaminated by Lm after this treatment. Federal regulators and manufacturers of RTE meats are working to find additional ways to control postprocess contamination by Lm in RTE meats. This research was initiated to validate combinations of antimicrobials that would produce an immediate lethality of at least 1 log of Lm on artificially contaminated frankfurters, and also suppress Lm growth to less than 2 logs throughout the extended shelf life at refrigerated temperatures (4 degrees C). Based on our studies, 22-ppm lauric arginate (LAE, ethyl-N-dodecanoyl-L-arginate hydrochloride) gave more than a 1-log reduction of Lm surface inoculated onto frankfurters within 12 h. The combination of either 1.8%/0.13% or 2.1%/0.15% potassium lactate/sodium diacetate (L/D) in combination with 22 ppm LAE caused more than a 2-log reduction at 12 h. Storage studies revealed that complementary interactions of L/D and LAE also met the 2nd requirement. This combination initially reduced Lm by 2 logs and suppressed growth to less than 2 logs even at the end of the 156-d storage life for frankfurters. These results confirmed that the combination of L/D with LAE as a postprocessing-prepackaging application could be useful in complying with the USDA's Alternative 1 that requires validation for the control of Lm on RTE frankfurters.
authors.affiliation: Dept. of Biological & Agricultural Engineering, Div. of Agriculture, Univ. of Arkansas, Fayetteville, AR 72704, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Martin
authors.forename: C L
authors.initials: CL
authors.lastname: Griffis
authors.forename: K L S
authors.initials: KL
authors.lastname: Vaughn
authors.forename: C A
authors.initials: CA
authors.lastname: O'Bryan
authors.forename: E C
authors.initials: EC
authors.lastname: Friedly
authors.forename: J A
authors.initials: JA
authors.lastname: Marcy
authors.forename: S C
authors.initials: SC
authors.lastname: Ricke
authors.forename: P G
authors.initials: PG
authors.lastname: Crandall
authors.forename: R Y
authors.initials: RY
authors.lastname: Lary
mesh_terms: D019342:Acetic Acid / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000378:metabolism*; D001120:Arginine / Q000008:administration & dosage / Q000378:metabolism*; D015169:Colony Count, Microbial; D005503:Food Additives / Q000378:metabolism; D005506:Food Contamination / Q000331:legislation & jurisprudence / Q000517:prevention & control; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology / Q000331:legislation & jurisprudence; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D007850:Lauric Acids / Q000008:administration & dosage / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000254:growth & development*; D008088:Listeriosis / Q000517:prevention & control; D008461:Meat Products / Q000382:microbiology*; D012677:Sensation; D019346:Sodium Acetate / Q000008:administration & dosage / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D005503:Food Additives; D007850:Lauric Acids; C030358:lauric acid; D019344:Lactic Acid; D019346:Sodium Acetate; D001120:Arginine; D019342:Acetic Acid