Pseudoclavibacter chungangensis sp. nov., isolated from activated sludge.
The taxonomic position of a Gram-positive, non-spore-forming strain, designated CAU 59(T), from activated sludge was investigated. Colony morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 59(T) possessed the characteristics of the genus Pseudoclavibacter. Comparative 16S rRNA gene sequence analysis showed sequence divergence values between strain CAU 59(T) and other described Pseudoclavibacter species of more than 3.6 %, and the strain formed a hitherto-unknown subline within the genus Pseudoclavibacter. DNA-DNA hybridization studies showed that strain CAU 59(T) displayed 20.9 % relatedness to its closest phylogenetic neighbour, Pseudoclavibacter helvolus DSM 20419(T). The DNA G+C content was 66.2 mol%. The phenotypic, chemotaxonomic and genotypic data indicated that strain CAU 59(T) represents a novel species of the genus Pseudoclavibacter, for which the name Pseudoclavibacter chungangensis sp. nov. is proposed. The type strain is CAU 59(T) (=KCTC 22691(T) =CCUG 58142(T)).
Cho
Sung-Lim
SL
Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
Jung
Min Young
MY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.|Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
Park
Mi-Hak
MH
Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
Chang
Young-Hyo
YH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Myung
Soon Chul
SC
Department of Urology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
Kim
Wonyong
W
Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D001483:Base Sequence; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S; D012722:Sewage