title: Online purification and determination of beta-antagonists in blood sample based on heart-cutting CE with electrochemical detection.
abstract: A novel method to detect beta-antagonists in rat blood was developed by combining heart-cutting 2-D CE in a single capillary with on-column electrochemical (EC) detection. The blood sample was purified in first dimension by non-micellar CZE separation. Then only a desirable fraction of the first dimension separation was transferred into the second dimension of the capillary by pressure. Finally, the purified sample was separated by MEKC. On-line dual concentration method, field amplified sample stacking and sweeping, was employed to accumulate the purified components that effectively counteracted the sample zone diffusion during the mobilization pressure step. A field decoupler joint and an EC detection cell were employed for on-column EC detection. After optimizing the analytical conditions, the RSD of peak height, peak area and migration time were 2.1-4.3%, 1.5-3.9% and 0.7-1.8% (n=10), respectively. This method has been successfully applied to determine beta-antagonists in postdosing rat blood sample. The pharmacokinetic study also demonstrated that the proposed heart-cutting 2-D CE with on-column EC detection was convenient, sensitive and would become an attractively alternative method for on-line sample cleanup and separation in complex samples.
authors.affiliation: Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, P. R. China.
authors.forename: Zhaoxiang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Zhang
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood*; D000818:Animals; D020374:Chromatography, Micellar Electrokinetic Capillary; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D051381:Rats; D012680:Sensitivity and Specificity
chemical_list: D000319:Adrenergic beta-Antagonists