title: Single nucleotide polymorphisms of 17beta-hydroxysteroid dehydrogenase type 7 gene: mechanism of estramustine-related adverse reactions?
abstract: OBJECTIVE
  To investigate the influence of single nucleotide polymorphisms (SNP) on transcription of the 17beta-hydroxysteroid dehydrogenase (HSD17B7) gene.
  
  
  METHODS
  Luciferase reporter genes containing a 5'-flanking of the HSD17B7 gene, as well as the sequence around the SNP, were transfected into LNCaP and DU145 cells. Then, luciferase assays were carried out.
  
  
  RESULTS
  The presence of the G allele resulted in an increase of transcriptional activity derived from the 5'-flanking region of the HSD17B7 gene by 270% and 370% in LNCaP and DU145 cells, respectively. Transcriptional activity of the HSD17B7 gene containing the G allele was higher than that of the C allele.
  
  
  CONCLUSIONS
  The transcriptional activity of the HSD17B7 gene containing the G allele is higher than that of the C allele. This difference in HSD17B7 expression may regulate the risk of peripheral edema as an adverse reaction induced by estramustine phosphate sodium.
authors.affiliation: Department of Clinical Evaluation of Drug Efficacy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ozeki
authors.forename: Megumi
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Motofumi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Tadaichi
authors.initials: T
authors.lastname: Kitamura
authors.forename: Risa
authors.initials: R
authors.lastname: Takayanagi
authors.forename: Haruko
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D018931:Antineoplastic Agents, Hormonal / Q000009:adverse effects*; D004487:Edema / Q000139:chemically induced* / Q000235:genetics*; D004961:Estramustine / Q000009:adverse effects*; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D018931:Antineoplastic Agents, Hormonal; D004961:Estramustine; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase