title: Production and characterization of cellulose by Acetobacter sp. V6 using a cost-effective molasses-corn steep liquor medium.
abstract: In order to reduce of the manufacturing cost of bacterial cellulose (BC), BC production by Acetobacter sp. V6 was investigated in shaking culture using molasses and corn steep liquor (CSL) as the sole carbon and nitrogen sources, respectively. The highest BC production was obtained with Ca3(PO4)2-treated molasses. Maximum BC yield (2.21+/-0.04 g/l) was obtained at 5% (w/v) total sugar in molasses. In improved medium containing molasses and CSL, BC production was observed in the medium after 1 day of incubation and increased rapidly thereafter with maximum yield (3.12+/-0.03 g/l) at 8 days. This value was approximately twofold higher than the yield in the complex medium. Physical properties of BC from the complex and molasses media were studied using Fourier-transform infrared (FT-IR) spectroscopy and X-ray diffractometer. By FT-IR, all the BC were found to be of cellulose type I, the same as typical native cellulose. The relative crystallinity of BC produced in the complex and molasses media were 83.02 and 67.27%, respectively. These results suggest that molasses and CSL can be useful low-cost substrates for BC production by Acetobacter sp. V6 without supplementation with expensive nitrogen complexes such as yeast extract and polypeptone, leading to the reduction in the production costs.
authors.affiliation: Department of Life Science & Environmental Biochemistry, Pusan National University, Miryang 627-706, South Korea.
authors.forename: Ho-Il
authors.initials: HI
authors.lastname: Jung
authors.forename: O-Mi
authors.initials: OM
authors.lastname: Lee
authors.forename: Jin-Ha
authors.initials: JH
authors.lastname: Jeong
authors.forename: Young-Dong
authors.initials: YD
authors.lastname: Jeon
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Park
authors.forename: Hong-Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Won-Gun
authors.initials: WG
authors.lastname: An
authors.forename: Hong-Joo
authors.initials: HJ
authors.lastname: Son
mesh_terms: D000091:Acetobacter / Q000201:enzymology*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000191:economics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008965:Molasses / Q000191:economics; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction; D003313:Zea mays / Q000378:metabolism*
chemical_list: D003470:Culture Media; D002482:Cellulose