Insecticide-induced changes in protein, RNA, and DNA contents in ovary and fat body of female Nilaparvata lugens (Hemiptera: Delphacidae).
The widespread use of insecticides is known to cause resurgence in Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) populations, a serious insect pest of rice, Orzya sativa L., crops in Asia. The current study investigated insecticide-induced changes in protein, RNA, and DNA levels in the ovary and fat body of adult female N. lugens. Potted rice plants were sprayed with different concentrations of imidacloprid, triazophos, and detamethrin. The results showed that all three insecticides caused a significant increase in protein and RNA contents in the ovary and fat body of adult females developed from nymphs fed on treated plants compared with those from on untreated plants, although there was no significant effect on DNA content. In general, protein and RNA contents in both ovary and fat body increased linearly with insecticide concentrations or days after emergence (DAEs) when they were applied. There was a significant correlation between protein content and RNA content in both ovary and fat body. In addition, the relationship between ratios of RNA to protein in ovary and fat body of adult female N. lugens and insecticide concentrations showed a significant positive correlation for most DAEs of imidacloprid and several DAEs of triazophos and deltamethrin treatments. These findings suggest that RNA in both ovary and fat body was more sensitive to insecticides and that the insecticide-induced changes in RNA content in turn influence protein synthesis in ovary and fat body. Consistently, RNA-to-protein ratios in ovary and fat body were approximately 0.42 in all treatments and controls.
Ge
Lin-Quan
LQ
Plant Protection College, Yangzhou University, Yangzhou 225009, People's Republic of China.
Hu
Jun-Huan
JH
Wu
Jin-Cai
JC
Yang
Guo-Qing
GQ
Gu
Hainan
H
D000818:Animals; D004247:DNA / Q000378:metabolism*; D005216:Fat Body / Q000187:drug effects*; D005260:Female; D006430:Hemiptera / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D019476:Insect Proteins / Q000378:metabolism*; D007306:Insecticides / Q000494:pharmacology*; D000073943:Neonicotinoids; D009570:Nitriles / Q000494:pharmacology; D009574:Nitro Compounds / Q000494:pharmacology; D063086:Organothiophosphates / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects*; D011722:Pyrethrins / Q000494:pharmacology; D012313:RNA / Q000378:metabolism*; D014230:Triazoles / Q000494:pharmacology
D007093:Imidazoles; D019476:Insect Proteins; D007306:Insecticides; D000073943:Neonicotinoids; D009570:Nitriles; D009574:Nitro Compounds; D063086:Organothiophosphates; D011722:Pyrethrins; D014230:Triazoles; C017180:decamethrin; C082359:imidacloprid; C012584:triazophos; D012313:RNA; D004247:DNA