title: Biological response of osteosarcoma cells to size-controlled nanostructured hydroxyapatite.
abstract: Osteosarcoma is a primary malignant bone tumor, most prevalent in children and adolescents, and is usually highly aggressive and eventually lethal. Despite multimodal therapies, there is no effective approach to treat this malignant disease. In this study, we observed the biological response of osteosarcoma cells to two kinds of hydroxyapatite nanoparticles (Nano HA), NanoHA-S and NanoHA-L. These nanospheres have the same crystallinity (phase) and morphology, but they differ in size. Cells treated with two kinds of Nano HA were inhibited and mainly led to apoptotic cell death. Caspase-9-dependent intrinsic apoptotic pathway plays a role. It was interesting that the suppression and the apoptosis of osteosarcoma cells was directly related to the size of nanoparticles and that the larger-sized Nano HA exhibited more effectiveness than the smaller one. This in vitro study suggested the potential of size-controlled calcium phosphate nanoparticles for use in therapeutic replacement and reconstruction of bone merits after osteosarcoma extraction.
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Shi
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Huimin
authors.initials: H
authors.lastname: Tao
authors.forename: Yurong
authors.initials: Y
authors.lastname: Cai
authors.forename: Ruikang
authors.initials: R
authors.lastname: Tang
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D012516:Osteosarcoma / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction