Homodimeric alkaline phosphatase located at Helicoverpa armigera midgut, a putative receptor of Cry1Ac contains alpha-GalNAc in terminal glycan structure as interactive epitope.
Helicoverpa armigera causes massive yield loss of various crops globally. Bacillus thuringiensis coded Cry1Ac toxin is effective against the pest. Through the present investigation, an alkaline phosphatase type putative receptor of the toxin was identified in the target insect gut BBMV. Lectin-ligand immunoblot assay detected the presence of alpha-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction.
Sarkar
Anindya
A
Plant Molecular and Cellular Genetics, Bose Institute, P-1/12, C.I.T. Scheme, VII-M, Kolkata 700054, India.
Hess
Daniel
D
Mondal
Hossain A
HA
Banerjee
Santanu
S
Sharma
Hari C
HC
Das
Sampa
S
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D001426:Bacterial Proteins; D002462:Cell Membrane / Q000378:metabolism; D002853:Chromatography, Liquid; D004064:Digestive System / Q000201:enzymology; D004731:Endotoxins / Q000378:metabolism* / Q000633:toxicity; D000939:Epitopes / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D011134:Polysaccharides / Q000378:metabolism*; D055503:Protein Multimerization; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D004731:Endotoxins; D000939:Epitopes; D019476:Insect Proteins; D011134:Polysaccharides; D000469:Alkaline Phosphatase