title: Identification of Burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody.
abstract: The genus Burkholderia includes many bacteria that cause serious human infections. As is the case with other Gram-negative bacteria, Burkholderia species produce LPS, which is an abundant component of the bacterial cell surface. Burkholderia cepacia complex (Bcc) bacteria (which include at least 17 separate species) produce LPS structures that are quite different. In an attempt to determine the degree of LPS epitope variation among Bcc species, a mAb was produced, designated 5D8, specific for the LPS of B. cepacia. Western blot analysis determined that mAb 5D8 was able to produce the classic 'ladder pattern' when used to probe B. cepacia and Burkholderia anthina lysates, although 5D8 did not produce this pattern with the other seven Bcc species tested. mAb 5D8 reacted with varying intensity to most but not all of the additional B. cepacia and B. anthina strains tested. Therefore, there seems to be significant epitope variation among Bcc LPS both between and within species. Additionally, mAb 5D8 reacted with a proteinase-K-sensitive 22 kDa antigen in all Bcc strains and also in a strain of Burkholderia pseudomallei.
authors.affiliation: Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, NV 89557, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: AuCoin
authors.affiliation: Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, NV 89557, USA.
authors.forename: Reva B
authors.initials: RB
authors.lastname: Crump
authors.affiliation: Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, NV 89557, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Thorkildson
authors.affiliation: Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, NV 89557, USA.
authors.forename: Dana E
authors.initials: DE
authors.lastname: Nuti
authors.affiliation: Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: LiPuma
authors.affiliation: Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, NV 89557, USA.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Kozel
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000378:metabolism*; D019121:Burkholderia Infections / Q000175:diagnosis / Q000382:microbiology; D042602:Burkholderia cepacia complex / Q000302:isolation & purification*; D008070:Lipopolysaccharides / Q000145:classification*; D051379:Mice; D008807:Mice, Inbred BALB C; D025202:Molecular Diagnostic Techniques / Q000379:methods; D011485:Protein Binding
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides