title: Age-dependent decrease in the interaction of beta-tubulin with estrogen receptor alpha transactivation domain in mouse brain.
abstract: In the previous paper, we reported that four proteins of 100 kDa, 80 kDa, 68 kDa and 50 kDa from the mouse brain interacted with estrogen receptor (ER) alpha-transactivation domain (TAD) and 68 kDa protein showed age and sex dependent changes. Here, we describe the identification of 50 kDa protein as beta-tubulin and changes in its interaction with age and sex in mouse brain. It is a microtubule-associated protein which binds to activation function (AF)-1 region of ERalpha-TAD and is involved in estrogen signaling. The extent of interaction of mouse ERalpha-TAD with beta-tubulin was higher in adult female as compared to old female and adult male. However, the expression of beta-tubulin showed no significant change with age and sex. Such age and sex dependent alteration in the interaction of beta-tubulin might account for the estrogen-mediated brain functions during aging.
authors.affiliation: Biochemistry and Molecular Biology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Ghosh
authors.forename: M K
authors.initials: MK
authors.lastname: Thakur
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D008970:Molecular Weight; D017434:Protein Structure, Tertiary; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015533:Transcriptional Activation; D014404:Tubulin / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; D014404:Tubulin