Anti-inflammatory activity of superoxide dismutase obtained from Debaryomyces hansenii on type II collagen induced arthritis in rats.
BACKGROUND
Rheumatoid arthritis is an autoimmune inflammatory disease of unknown etiology, free radicals have been implicated in the genesis and perpetuation of damage in this pathology.


OBJECTIVE
To evaluate the anti-inflammatory effect of Cu,Zn-superoxide dismutase (SOD) obtained from two different sources (bovine erythrocytes, Be-SOD, and Debaryomyces hansenii, Dh-SOD) with Type II Collagen-induced Arthritis model in rats.


METHODS
Arthritis was induced by repeated injection of a porcine type II collagen-incomplete Freund adjuvant suspension on the back of Dark Augui (DA) rats. Arthritis was clinically evaluated throughout the study. Body weight was determined at three different times. Two different doses for each treatment (Be-SOD, Dh-SOD) were tested: 100 and 1,000 U/kg. At the end of the trial (day 28), histological analyses of the most inflamed ankle joint, as well as serum anti-collagen antibodies, were determined.


RESULTS
Both sources of SOD decreased, although to a different extent, the incidence and severity of the disease. Arthritis score was lower in all treatments, except for the low dose of Be-SOD. Groups receiving either source of SOD showed a significant weight increase compared to the placebo group. Histological damage was similar in all groups. Only the group that received the highest dose of Dh-SOD showed a significant lower antibody titer; nevertheless, no correlation appears to derive from arthritis score and antibody titer.


CONCLUSIONS
Our findings suggest that, although unable to counteract the arthritis syndrome, SOD may still be beneficial due to its anti-inflammatory activity. In the case of Dh-SOD, the best effect was observed at the highest dose tested.
García-González
Adolfo
A
Internal Medicine Service, Instituto Mexicano del Seguro Social (HGZMF 1), La Paz, B.C.S. adolfo.garcia@imss.gob.mx
Lotz
Martin
M
Ochoa
Jose L
JL
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D018501:Antirheumatic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000097:blood / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control; D001172:Arthritis, Rheumatoid; D001323:Autoantibodies / Q000097:blood; D002417:Cattle; D024043:Collagen Type II / Q000633:toxicity; D055165:Debaryomyces / Q000201:enzymology*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005355:Fibrosis; D005656:Fungal Proteins / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D006965:Hyperplasia; D007274:Injections, Intraperitoneal; D051381:Rats; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D018501:Antirheumatic Agents; D001323:Autoantibodies; D024043:Collagen Type II; D005656:Fungal Proteins; D013482:Superoxide Dismutase