Expression of human beta-defensin-2 gene induced by CpG-DNA in human B cells.
Defensins have a broad range of antimicrobial activity against bacteria, fungi, and viruses. The expression of human beta-defensin-2 (hBD-2) is prevalently observed in epithelial cells and is induced by bacterial infection. Here, we have shown that the expression of the hBD-2 gene and release of hBD-2 protein into the medium is up-regulated in response to CpG-DNA in human B cell line RPMI 8226. The induction of hBD-2 was dependent on CG sequence and phosphorothioate backbone-modification. This was also confirmed in primary human lymphocytes. To shed light on the molecular mechanism involved in hBD-2 induction by CpG-DNA, we examined the contribution of the NF-kappaB signaling pathway in RPMI 8226 cells. Suppression of MyD88 function and inhibition of NF-kappaB nuclear localization blocked hBD-2 induction. The NF-kappaB pathway inhibitors also abolished hBD-2 induction. These results may contribute to a better understanding on the therapeutic effects of CpG-DNA against infectious diseases.
Han
Su Ho
SH
Department of Microbiology, College of Medicine, Hallym University, Gangwon-do 200-702, Republic of Korea.
Kim
Young-Eun
YE
Park
Jeong-A
JA
Park
Jae-Bong
JB
Kim
Yong-Sun
YS
Lee
Younghee
Y
Choi
Ihn-Geun
IG
Kwon
Hyung-Joo
HJ
D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D015854:Up-Regulation; D023083:beta-Defensins / Q000235:genetics*
C408982:CPG-oligonucleotide; C106641:DEFB4A protein, human; D009838:Oligodeoxyribonucleotides; D023083:beta-Defensins