title: Combined inactivation of lon and ycgE decreases multidrug susceptibility by reducing the amount of OmpF porin in Escherichia coli.
abstract: Transposon inactivation of ycgE, a gene encoding a putative transcriptional regulator, led to decreased multidrug susceptibility in an Escherichia coli lon mutant. The multidrug susceptibility phenotype (e.g., to tetracycline and beta-lactam antibiotics) required the inactivation of both lon and ycgE. In this mutant, a decreased amount of OmpF porin contributes to the lowered drug susceptibility, with a greater effect at 26 degrees C than at 37 degrees C.
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.
authors.forename: Valérie
authors.initials: V
authors.lastname: Duval
authors.forename: Hervé
authors.initials: H
authors.lastname: Nicoloff
authors.forename: Stuart B
authors.initials: SB
authors.lastname: Levy
mesh_terms: D004268:DNA-Binding Proteins / Q000502:physiology; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000502:physiology*; D008074:Lipoproteins / Q000502:physiology; D026901:Membrane Transport Proteins / Q000502:physiology; D008826:Microbial Sensitivity Tests; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000502:physiology; D018272:Porins / Q000502:physiology*; D049070:Protease La / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D018272:Porins; D049070:Protease La; C083497:AcrA protein, E coli; C083496:AcrB protein, E coli; C080002:MarA protein, E coli; C109237:OmpF protein; C483567:Lon protein, E coli