title: Diacerein decreases TNF-alpha and IL-1beta levels in peritoneal fluid and prevents Baker's yeast-induced fever in young rats.
abstract: OBJECTIVE
  To investigate the effect of diacerein, an anti-inflammatory drug, on body temperature and protocols of fever induction in male Wistar rats.
  
  
  METHODS
  The effect of diacerein (5.0 mg/kg, s.c.) on rectal temperature (T (R)) changes induced by Baker's yeast (0.135 g/kg, i.p.) and PGE(2) (10 ng/animal, i.t.) was evaluated. T (R) changes were recorded over time. The leukocyte count and TNF-alpha and IL-1beta content were evaluated in the peritoneal fluid by means of optical microscopy and enzyme immunoassay (ELISA kits), respectively.
  
  
  RESULTS
  The administration of diacerein to febrile animals attenuated Baker's yeast-induced fever but did not alter prostaglandin E(2)-induced fever. Diacerein prevented the development of Baker's yeast-induced fever and significantly attenuated the increase in peritoneal leukocytes and decreased IL-1beta and TNF-alpha levels in peritoneal fluid.
  
  
  CONCLUSIONS
  These data suggest that diacerein partially protects against Baker's yeast-induced fever and peritoneal leukocyte migration, and indicate that this effect appears to be due to inhibition of release of cytokines (such as TNF-alpha and IL-1beta).
authors.affiliation: Department of Physiology, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Juliana Saibt Martins
authors.initials: JS
authors.lastname: Pasin
authors.forename: Ana Paula Oliveira
authors.initials: AP
authors.lastname: Ferreira
authors.forename: André Luis Lopes
authors.initials: AL
authors.lastname: Saraiva
authors.forename: Viviane
authors.initials: V
authors.lastname: Ratzlaff
authors.forename: Rosália
authors.initials: R
authors.lastname: Andrighetto
authors.forename: Jorgete
authors.initials: J
authors.lastname: Tomazetti
authors.forename: Daiana Silva
authors.initials: DS
authors.lastname: Avila
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
authors.forename: Maribel Antonello
authors.initials: MA
authors.lastname: Rubin
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira
authors.forename: Adair Roberto Soares
authors.initials: AR
authors.lastname: Santos
authors.forename: Carlos Fernando
authors.initials: CF
authors.lastname: Mello
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001202:Ascitic Fluid / Q000378:metabolism*; D001831:Body Temperature / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D015232:Dinoprostone / Q000009:adverse effects; D004195:Disease Models, Animal; D005334:Fever / Q000139:chemically induced / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000378:metabolism*; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D000880:Anthraquinones; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; C025292:diacerein; D015232:Dinoprostone