title: Assessment of ligand binding residue predictions in CASP8.
abstract: Here we detail the assessment process for the binding site prediction category of the eighth Critical Assessment of Protein Structure Prediction experiment (CASP8). Predictions were only evaluated for those targets that bound biologically relevant ligands and were assessed using the Matthews Correlation Coefficient. The results of the analysis clearly demonstrate that three predictors from two groups (Lee and Sternberg) stand out from the rest. A further two groups perform well over subsets of metal binding or nonmetal ligand binding targets. The best methods were able to make consistently reliable predictions based on model structures, though it was noticeable that the two targets that were not well predicted were also the hardest targets. The number of predictors that submitted new methods in this category was highly encouraging and suggests that current technology is at the level that experimental biochemists and structural biologists could benefit from what is clearly a growing field.
authors.affiliation: Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: López
authors.forename: Iakes
authors.initials: I
authors.lastname: Ezkurdia
authors.forename: Michael L
authors.initials: ML
authors.lastname: Tress
mesh_terms: D001665:Binding Sites; D019295:Computational Biology / Q000379:methods*; D008024:Ligands; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D008024:Ligands; D011506:Proteins