title: Determination of six pesticides in the medicinal herb Cordia salicifolia by matrix solid-phase dispersion and gas chromatography/mass spectrometry.
abstract: A simple and effective extraction method based on matrix solid-phase dispersion was developed for acephate, chlorpropham, pyrimicarb, bifenthrin, tetradifon, and phosalone in leaves of the medicinal plant Cordia salicifolia, whose extracts are commercialized in Brazil as diuretic, appetite suppressant, and weight loss products. The determination method was GC/MS with selected-ion monitoring. Different parameters of the method were evaluated, such as type of solid phase (C18, alumina, silica gel, and Florisil) and the amount of solid phase and eluent (dichloromethane, ethyl acetate, chloroform, and cyclohexane). The best results were obtained using 0.5 g herb sample, 0.5 g neutral alumina as the dispersant sorbent, 0.5 g C18 as the cleanup sorbent, and cyclohexane-dichloromethane (3 + 1, v/v) as the eluting solvent. The method was validated using herb samples fortified with pesticides at different concentration levels (0.3, 0.5, and 1.0 mg/kg). Average recoveries (seven replicates) ranged from 67.7 to 129.9%, with relative standard deviations between 6.3 and 26%. Detection and quantitation limits for the herb ranged from 0.10 to 0.15 and 0.15 to 0.25 mg/kg, respectively.
authors.affiliation: Universidade Federal de Sergipe, Departamento de Química, Av. Marechal Rondon s/no, 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Pedro Henrique Viana
authors.initials: PH
authors.lastname: de Carvalho
authors.forename: Vanessa de Menezes
authors.initials: Vde M
authors.lastname: Prata
authors.forename: Péricles Barreto
authors.initials: PB
authors.lastname: Alves
authors.forename: Sandro
authors.initials: S
authors.lastname: Navickiene
mesh_terms: D001938:Brazil; D031213:Cordia / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents
chemical_list: D010573:Pesticide Residues; D010575:Pesticides; D012997:Solvents