Flavins contained in yeast extract are exploited for anodic electron transfer by Lactococcus lactis.
Cyclic voltammograms of yeast extract-containing medium exhibit a clear redox peak around -0.4V vs. Ag|AgCl. Fermentative bacterium Lactococcus lactis was hereby shown to exploit this redox compound for extracellular electron transfer towards a graphite anode using glucose as an electron donor. High performance liquid chromatography revealed that this may be a flavin-type compound. The ability of L. lactis to exploit exogenous flavins for anodic glucose oxidation was confirmed by tests where flavin-type compounds were supplied to the bacterium in well defined media. Based on its mid-point potential, riboflavin can be regarded as a near-optimal mediator for microbially catalyzed anodic electron transfer. Riboflavin derivative flavin mononucleotide (FMN) was also exploited by L. lactis as a redox shuttle, unlike flavin adenine dinucleotide (FAD), possibly due to the absence of a specific transporter for the latter. The use of yeast extract in microbial fuel cell media is herein discouraged based on the related unwanted artificial addition of redox mediators which may distort experimental results.
Masuda
Masaki
M
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8205, Japan. kinniku@kais.kyoto-u.ac.jp
Freguia
Stefano
S
Wang
Yung-Fu
YF
Tsujimura
Seiya
S
Kano
Kenji
K
D016477:Artifacts; D002021:Buffers; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004583:Electrons; D005110:Extracellular Space / Q000378:metabolism; D005285:Fermentation; D005415:Flavins / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006108:Graphite / Q000737:chemistry; D013294:Lactococcus lactis / Q000166:cytology / Q000378:metabolism*; D012256:Riboflavin / Q000378:metabolism; D015003:Yeasts / Q000737:chemistry*
D002021:Buffers; D005415:Flavins; D006108:Graphite; D005947:Glucose; D012256:Riboflavin