title: Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.
abstract: Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.
authors.affiliation: David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
authors.forename: Araceli
authors.initials: A
authors.lastname: Espinosa-Jeffrey
authors.forename: Dustin R
authors.initials: DR
authors.lastname: Wakeman
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.forename: Evan Y
authors.initials: EY
authors.lastname: Snyder
authors.forename: Jean
authors.initials: J
authors.lastname: de Vellis
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001681:Biological Assay; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D015925:Cryopreservation; D006801:Humans; D009186:Myelin Sheath / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology
chemical_list: D019006:Neural Cell Adhesion Molecules