Epithelial cells are the major site of hydroxysteroid (17beta) dehydrogenase 2 and androgen receptor expression in fetal mouse lungs during the period overlapping the surge of surfactant.
Many genes involved in the peripheral metabolism of androgens, including hydroxysteroid (17beta) dehydrogenases (HSD17B) 2 and 5, steroid 5alpha reductase 1, and 3alpha-HSD, are expressed in the developing lung. Because lung development is delayed by androgens and pathologies related to lung immaturity are major concerns for preterm neonates, we are interested in the elucidation of the androgen metabolism in developing lung. In the present report we have identified the cell types expressing HSD17B2 (testosterone into androstenedione) and androgen receptor in normal male and female mouse developing lung between the gestation days 15.5 and 17.5. In situ hybridization and immunohistochemistry revealed that HSD17B2 is expressed in epithelial cells of respiratory and conducting zones, and in mesenchymal cells. The androgen receptor protein was observed in the same cell types that HSD17B2, and in alpha-smooth muscle actin-positive cells surrounding arteries. No difference was observed for the location of HSD17B2 and androgen receptor expression at any time points studied, or according to sex. Taken together, our results are in concordance with the hypothesis that in mouse fetal lungs the level of androgen receptor occupancy is finely tuned by local HSD17B2 expression.
Plante
Julie
J
Laboratory of Ontogeny and Reproduction, Centre Hospitalier Universitaire de Québec (CHUQ), Pavillon CHUL, Faculty of Medicine, Laval University, Québec, Canada.
Simard
Marc
M
Rantakari
Pia
P
Côté
Mélissa
M
Provost
Pierre R
PR
Poutanen
Matti
M
Tremblay
Yves
Y
D015067:17-Hydroxysteroid Dehydrogenases; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004847:Epithelial Cells / Q000201:enzymology / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008168:Lung / Q000196:embryology* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D017931:DNA Primers; D011663:Pulmonary Surfactants; D011944:Receptors, Androgen; D011994:Recombinant Proteins; D015067:17-Hydroxysteroid Dehydrogenases; C032690:3-alpha-(17-beta)-hydroxysteroid dehydrogenase (NAD(+)); D004960:Estradiol Dehydrogenases