title: Ethyl alcohol extracts of Hizikia fusiforme sensitize AGS human gastric adenocarcinoma cells to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis.
abstract: Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis has been reported in some cancer cells, including AGS human gastric adenocarcinoma cells. Hizikia fusiforme is a commonly used brown seaweed species in Korea that possesses potent antibacterial, antifungal, and anti-inflammatory activities. In this study, we demonstrated that treatment with TRAIL in combination with subtoxic concentrations of ethyl alcohol extract of H. fusiforme (EAHF) sensitized TRAIL-resistant AGS cells to TRAIL-mediated apoptosis. Combined treatment with EAHF and TRAIL increased chromatin condensation, DNA fragmentation, and sub-G1-phase DNA content. The restored sensitivity to TRAIL-induced apoptosis appeared to be correlated with the modulation of Bcl-2 family proteins and activation of caspases, which resulted in the cleavage of poly(ADP-ribose)polymerase. Taken together, the use of EAHF in combination with TRAIL may be an effective and selective anticancer strategy via suppressing the resistance to TRAIL-induced apoptosis in some tumor cell lines, including AGS cells.
authors.affiliation: Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Jeju, Republic of Korea.
authors.forename: Tae-Yeon
authors.initials: TY
authors.lastname: Kim
authors.forename: Cheng-Yun
authors.initials: CY
authors.lastname: Jin
authors.forename: Gi-Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Il-Whan
authors.initials: IW
authors.lastname: Choi
authors.forename: Yong Kee
authors.initials: YK
authors.lastname: Jeong
authors.forename: Taek-Jeong
authors.initials: TJ
authors.lastname: Nam
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004359:Drug Therapy, Combination; D006801:Humans; D007723:Korea; D008024:Ligands; D000459:Phaeophyceae; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology / Q000627:therapeutic use*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002843:Chromatin; D008024:Ligands; D010936:Plant Extracts; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D051028:bcl-2-Associated X Protein; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases