title: Caveolin-1 is an important factor for the metastasis and proliferation of human small cell lung cancer NCI-H446 cell.
abstract: Caveolin-1 (CAV-1) has been reported to play an important role in the development of a variety of human cancers. CAV-1 expression is revealed to be reduced or absent in the malignant tumor cells of small cell lung cancers (SCLC). This study was performed to investigate the influences of the stable expression of CAV-1 on the metastasis and proliferation of SCLC in vitro. The wild-type CAV-1 gene was successfully transfected into the NCI-H446 cells and was stably expressed in the NCI-H446 cells. The effects of CAV-1 on the morphology, proliferation, and metastasis potential for NCI-H446 cell were evaluated by crystal violet staining, MTT analysis, transwell assay, and scratch wound assay, respectively. Western blot and gelatin zymography were used to examine the expression changes of the metastasis-related MMP-3 and E-cadherin. Stable expression of CVA-1 was observed in the H446-CAV-1 cells, which enlarged the cell shape with filopodia. The proliferation of H446-CAV-1 was inhibited, while its migration and invasion abilities were promoted in vitro. The re-expression of CAV-1 reduced the expression of E-cadherin, while it increased the protein expression and enzyme activity of MMP-3. Taken together, the cellular proliferation of the NCI-H446 could be inhibited by the re-expression of CAV-1. CAV-1 might increase the cell metastasis potential through the interaction with E-cadherin and MMP-3 genes. These in vitro findings confirm the involvement of CAV-1 in the proliferation and metastasis of SCLC.
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian 116044, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yeh
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Ming-Zhong
authors.initials: MZ
authors.lastname: Sun
authors.forename: Shujuan
authors.initials: S
authors.lastname: Shao
authors.forename: Lihong
authors.initials: L
authors.lastname: Hao
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.forename: Linlin
authors.initials: L
authors.lastname: Gong
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
mesh_terms: D015820:Cadherins / Q000378:metabolism; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D048430:Cell Shape; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D055752:Small Cell Lung Carcinoma / Q000378:metabolism*
chemical_list: D015820:Cadherins; D051242:Caveolin 1; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3