title: Internal standard-based analysis of microarray data. Part 1: analysis of differential gene expressions.
abstract: Genome-scale microarray experiments for comparative analysis of gene expressions produce massive amounts of information. Traditional statistical approaches fail to achieve the required accuracy in sensitivity and specificity of the analysis. Since the problem can be resolved neither by increasing the number of replicates nor by manipulating thresholds, one needs a novel approach to the analysis. This article describes methods to improve the power of microarray analyses by defining internal standards to characterize features of the biological system being studied and the technological processes underlying the microarray experiments. Applying these methods, internal standards are identified and then the obtained parameters are used to define (i) genes that are distinct in their expression from background; (ii) genes that are differentially expressed; and finally (iii) genes that have similar dynamical behavior.
authors.affiliation: Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. igor-dozmorov@omrf.org
authors.forename: Igor
authors.initials: I
authors.lastname: Dozmorov
authors.forename: Ivan
authors.initials: I
authors.lastname: Lefkovits
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000592:standards*; D012015:Reference Standards