Muscle differentiation in a colonial ascidian: organisation, gene expression and evolutionary considerations.
BACKGROUND
Ascidians are tunicates, the taxon recently proposed as sister group to the vertebrates. They possess a chordate-like swimming larva, which metamorphoses into a sessile adult. Several ascidian species form colonies of clonal individuals by asexual reproduction. During their life cycle, ascidians present three muscle types: striated in larval tail, striated in the heart, and unstriated in the adult body-wall.


RESULTS
In the colonial ascidian Botryllus schlosseri, we investigated organisation, differentiation and gene expression of muscle beginning from early buds to adults and during zooid regression. We characterised transcripts for troponin T (BsTnT-c), adult muscle-type (BsMA2) and cytoplasmic-type (BsCA1) actins, followed by in situ hybridisation (ISH) on sections to establish the spatio-temporal expression of BsTnT-c and BsMA2 during asexual reproduction and in the larva. Moreover, we characterised actin genomic sequences, which by comparison with other metazoans revealed conserved intron patterns.


CONCLUSIONS
Integration of data from ISH, phalloidin staining and TEM allowed us to follow the phases of differentiation of the three muscle kinds, which differ in expression pattern of the two transcripts. Moreover, phylogenetic analyses provided evidence for the close relationship between tunicate and vertebrate muscle genes. The characteristics and plasticity of muscles in tunicates are discussed.
Degasperi
Valentina
V
Dipartimento di Biologia, Università degli Studi di Padova, Via Ugo Bassi 58/B, 35131, Padova, Italy. valentina.degasperi@unipd.it
Gasparini
Fabio
F
Shimeld
Sebastian M
SM
Sinigaglia
Chiara
C
Burighel
Paolo
P
Manni
Lucia
L
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D004625:Embryo, Nonmammalian / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D009132:Muscles / Q000378:metabolism / Q000648:ultrastructure; D010802:Phylogeny; D020107:Troponin T / Q000235:genetics / Q000378:metabolism; D014561:Urochordata / Q000196:embryology / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure
D000199:Actins; D009124:Muscle Proteins; D020107:Troponin T