title: Boron containing two-photon absorbing chromophores. 2. Fine tuning of the one- and two-photon photophysical properties of pyrazabole based fluorescent bioprobes.
abstract: New boron containing two-photon absorbing fluorophores have been prepared. Centered on a pyrazabole central core, various conjugated systems and end groups were investigated to modulate their physicochemical properties (alkoxy, diphenylamino, and boron dipyromethene groups). One and two-photon photophysical characterizations were performed, showing efficient fluorescence in organic solvents. High two-photon absorption cross sections were determined in the 500-800 nm range. Two-photon excited microscopy images were also obtained with these new boron containing fluorescent bioprobes with laser intensities in the milliwatt range.
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg (UMR CNRS 7213), Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Hayek
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bolze
authors.forename: Cyril
authors.initials: C
authors.lastname: Bourgogne
authors.forename: Patrice L
authors.initials: PL
authors.lastname: Baldeck
authors.forename: Pascal
authors.initials: P
authors.lastname: Didier
authors.forename: Youri
authors.initials: Y
authors.lastname: Arntz
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Nicoud
mesh_terms: D000042:Absorption; D015374:Biosensing Techniques / Q000379:methods*; D001895:Boron / Q000737:chemistry*; D003116:Color; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D001895:Boron