Rapid detection of rpoB gene mutations in rif-resistant M. tuberculosis isolates by oligonucleotide microarray.
OBJECTIVE
To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray.


METHODS
Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray.


RESULTS
Of the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes.


CONCLUSIONS
Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.
Sun
Ai-Hua
AH
Department of Basic Medicine, Zhejiang Medical College, Hangzhou 310053, Zhejiang, China.
Fan
Xing-Li
XL
Li
Li-Wei
LW
Wang
Li-Fang
LF
Ans
Wen-Ying
WY
Yan
Jie
J
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012293:Rifampin / Q000494:pharmacology*
D000904:Antibiotics, Antitubercular; D001426:Bacterial Proteins; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin