Strong muscle-specific regulatory cassettes based on multiple copies of the human slow troponin I gene upstream enhancer.
High-level tissue-specific expression of recombinant proteins in muscle is an important issue for several therapeutic applications. To achieve this goal, we generated several constructs containing one to five copies of the upstream enhancer (USE) of 160-bp of the human slow troponin I gene, linked to that gene's minimal promoter. We also tested constructs made with one to four copies of a 100-bp deletion of USE (DeltaUSE) reported to drive pan-muscle-specific expression in transgenic mice. These constructs were evaluated by measuring the activity of the reporter gene beta-galactosidase (beta-gal). In cell culture, these multimerized enhancers retained tissue specificity and their transcriptional strength increased with the number of enhancer copies. In myotube cultures (which still contain nondifferentiated cells), constructs containing four and five USE copies were stronger than the cytomegalovirus (CMV) early enhancer/promoter and slightly weaker than the hybrid CMV enhancer/beta-actin (CB) promoter. Those containing three USE, or four DeltaUSE copies were similar in strength to CMV. After electrotransfer of plasmid DNA into the mouse tibialis anterior muscle, the strengths of the two constructs (USEx3 and DeltaUSEx3) were tested; as measured by beta-gal activity in the total muscle lysate and by the number of transduced fibers, they were similar to CMV and CB. Muscle fiber typing, after electrotransfer of the soleus muscle, showed that DeltaUSEx3 and USEx3 were active in slow and fast fibers. The tissue specificity of these two constructs was also evaluated by hydrodynamic plasmid injection through the tail vein. Although significant beta-gal expression was measured in the liver when CMV was tested, no expression above background level was detected with USEx3 and DeltaUSEx3. The strength, muscle specificity, and small size of these transcriptional elements render them very attractive for gene therapy applications.
Blain
Marilyne
M
Biotechnology Research Institute , National Research Council Canada, Montreal, Quebec H4P 2R2, Canada.
Zeng
Yue
Y
Bendjelloul
Mehdi
M
Hallauer
Patricia L
PL
Kumar
Angela
A
Hastings
Kenneth E M
KE
Karpati
George
G
Massie
Bernard
B
Gilbert
Rénald
R
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D018628:Gene Dosage / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D017930:Genes, Reporter; D005818:Genetic Engineering; D006801:Humans; D051379:Mice; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology / Q000378:metabolism; D018657:Muscle Fibers, Slow-Twitch / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D019210:Troponin I / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
D019210:Troponin I; D001616:beta-Galactosidase