Involvement of NAD(P)H:quinone oxidoreductase 1 and superoxide dismutase polymorphisms in ulcerative colitis.
Inflammatory bowel disease is a multifactorial disease. Oxidative stress has been thought to be one of etiologic factor for inflammatory bowel disease. The genes superoxide dismutase (SOD2) and NAD(P)H:quinone oxidoreductase 1 (NQO1) are involved in inflammation and oxidative stress. The purpose of the present case-control study with 134 patients with ulcerative colitis (UC) and 125 healthy controls was to determine whether polymorphisms of these genes, the NQO1 C609T and the SOD2 Ala-9Val, are associated with the risk of UC and influence the clinical characteristics. These polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphisms and direct sequencing. In patients showing steroid resistance, the number with the NQO1 T/T genotype was significantly higher than other genotypes (odds ratio 9.45, 95% confidence interval 2.46-41.6, p = 0.002). In the patients whose onset of UC was age 20 years or younger, more patients had SOD2 T/T genotype than the other genotypes (odds ratio 6.46, 95% confidence interval 0.82-51.0). No association between these polymorphisms and UC risk was apparent. The NQO1 C609T polymorphism may influence steroid resistance of UC patients, while the SOD2 Ala-9Val polymorphism may influence age of onset of UC. Oxidative stress may influence the clinical features of UC.
Kosaka
Toshihito
T
Department of Internal Medicine, Second Teaching Hospital Fujita Health University School of Medicine, Nagoya 454-8509, Japan.
Yoshino
Junji
J
Inui
Kazuo
K
Wakabayashi
Takao
T
Kobayashi
Takashi
T
Watanabe
Shinya
S
Hayashi
Shigekazu
S
Hirokawa
Yoshifumi
Y
Shiraishi
Taizo
T
Yamamoto
Takayuki
T
Tsuji
Mayumi
M
Katoh
Takahiko
T
Watanabe
Masatoshi
M
D000328:Adult; D017668:Age of Onset; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000235:genetics*; D055815:Young Adult; D000099156:Superoxide Dismutase 2
D016660:NAD(P)H Dehydrogenase (Quinone); D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C447479:NQO1 protein, human