Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand.
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.
Thongsahuan
Sorawat
S
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Baimai
Visut
V
Otsuka
Yasushi
Y
Saeung
Atiporn
A
Tuetun
Benjawan
B
Jariyapan
Narissara
N
Suwannamit
Sittiporn
S
Somboon
Pradya
P
Jitpakdi
Atchariya
A
Takaoka
Hiroyuki
H
Choochote
Wej
W
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D003433:Crosses, Genetic; D004272:DNA, Mitochondrial / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005260:Female; D005843:Geography; D007621:Karyotyping; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013785:Thailand
D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer