title: Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes.
abstract: The aim of the present study was to demonstrate the mitogenic and differentiating properties of platelet-rich plasma releasates (PRPr) on human chondrocytes in mono- and three-dimensional cultures. In order to assess if PRPr supplementation could maintain the chondrocyte phenotype or at least inhibit the cell de-differentiation even after several days in culture, we performed a proteomic study on several cell cultures independently grown, for different periods of time, in culture medium with FCS, human serum (HS), and releasates obtained from PRP and platelet-poor plasma (PPP). We found that PRP treatment actually induced in chondrocytes the expression of proteins (some of which novel) involved in differentiation.
authors.affiliation: Sezione di Reumatologia, Dipartimento di Medicina Clinica e Scienze Immunologiche, Università di Siena, Policlinico Le Scotte, Siena, Italy. spreafico@unisi.it
authors.forename: Adriano
authors.initials: A
authors.lastname: Spreafico
authors.forename: Federico
authors.initials: F
authors.lastname: Chellini
authors.forename: Bruno
authors.initials: B
authors.lastname: Frediani
authors.forename: Giulia
authors.initials: G
authors.lastname: Bernardini
authors.forename: Silvia
authors.initials: S
authors.lastname: Niccolini
authors.forename: Tommaso
authors.initials: T
authors.lastname: Serchi
authors.forename: Giulia
authors.initials: G
authors.lastname: Collodel
authors.forename: Alessandro
authors.initials: A
authors.lastname: Paffetti
authors.forename: Vittorio
authors.initials: V
authors.lastname: Fossombroni
authors.forename: Mauro
authors.initials: M
authors.lastname: Galeazzi
authors.forename: Roberto
authors.initials: R
authors.lastname: Marcolongo
authors.forename: Annalisa
authors.initials: A
authors.lastname: Santucci
mesh_terms: D001792:Blood Platelets / Q000378:metabolism; D002358:Cartilage, Articular / Q000378:metabolism*; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D003470:Culture Media; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D010452:Peptide Biosynthesis; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D040901:Proteomics
chemical_list: D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor