Cell culture in vivo by means of diffusion chamber system.
In a diffusion chamber (DC) system, cells are cultured in vivo - hence making it possible to minimize infection and foreign material contamination. In view of this merit, we devised a technique to combine a DC system and a scaffold to the end of incubating sufficient host cells for grafting. In the present study, PLGA sponge and rat bone marrow cells were encapsulated inside a DC and then placed inside the abdominal cavities of rats. DCs were removed at two or four weeks after grafting. At four weeks after grafting, fibrous and calcified tissue matching the shape of the PLGA sponge was formed. These results suggested that the PLGA sponge was an effective scaffolding material in inducing three-dimensional tissue formation and that combination with a DC system resulted in a cell mass matching the scaffold shape. In addition, the cells were cultured in vivo - which meant that DC culturing did not require special incubation facilities or technologies after grafting.
Nakano
Kenjiro
K
Departments of Dental Materials Science School of Dentistry, Aichi-Gakuin University. kenjiro@dpc.aichi-gakuin.ac.jp
Hayashi
Tatsuhide
T
Kawai
Hideki
H
Takei
Yukiko
Y
Sato
Yosuke
Y
Ando
Kimitoshi
K
Ono
Yuzo
Y
Jinno
Satoshi
S
Kawakami
Toshiyuki
T
Maeda
Hatuhiko
H
Kawai
Tatsushi
T
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells; D002118:Calcium / Q000032:analysis; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D015194:Diffusion Chambers, Culture; D019344:Lactic Acid; D008297:Male; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002118:Calcium