title: Ferrite-silica-insulin nanocomposites (FeSINC) for glucose reduction.
abstract: Proteins find a more stable environment upon encapsulation in a silica host, because of the polymeric silica frame that grows around the macromolecule and protects them from denaturation. Silica-insulin nanocomposite (SINC) and ferrite-coated SINC (FeSINC) was prepared by polyelectrolytic condensation of silica precursor on insulin and they were studied for their ability to control glucose levels. SINC was prepared by acid-base-catalyzed polymerization in the presence of insulin at room temperature by a modified Stober's process. FeSINC nanoparticles were prepared by coprecipitation of both ferric and ferrous salts on the bovine insulin loaded silica nanoparticle. The presence of ferrite coating in FeSINC was identified using a vibrating sample magnetometer and quantified from XRF study. The intermolecular interactions in these nanocomposites were studied by FTIR and Raman spectroscopy. An in vivo study indicated that FeSINC was biologically active in reducing glucose levels as compared to SINC.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai-400076, India.
authors.forename: Neelam
authors.initials: N
authors.lastname: Dwivedi
authors.lastname: Arunagirinathan
authors.forename: Somesh
authors.initials: S
authors.lastname: Sharma
authors.forename: Jayesh
authors.initials: J
authors.lastname: Bellare
mesh_terms: D000327:Adsorption; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle; D005290:Ferric Compounds / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry* / Q000494:pharmacology*; D008854:Microscopy, Electron; D053761:Nanocomposites / Q000737:chemistry*; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D001786:Blood Glucose; D005290:Ferric Compounds; D007328:Insulin; C001215:ferrite; D012822:Silicon Dioxide; D005947:Glucose