Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices- RiT-DNA carrot root mycorrhizas.
To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA-like protein, an oxido-reductase, a heat-shock protein and an ATP synthase beta mitochondrial precursor. The possible fungal origin of a MYK15-like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.
Dumas-Gaudot
Eliane
E
UMR INRA/CNRS UB, INRA-CMSE, Dijon, France. dumas@epoisses.inra.fr
Valot
Benoît
B
Bestel-Corre
Gwénaëlle
G
Recorbet
Ghislaine
G
St-Arnaud
Marc
M
Fontaine
Bastien
B
Dieu
Marc
M
Raes
Martine
M
Saravanan
Ramu Subramania
RS
Gianinazzi
Silvio
S
D018552:Daucus carota / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005656:Fungal Proteins / Q000032:analysis*; D055137:Glomeromycota / Q000737:chemistry* / Q000254:growth & development; D013058:Mass Spectrometry / Q000379:methods; D038821:Mycorrhizae / Q000254:growth & development*; D018517:Plant Roots / Q000382:microbiology*; D020543:Proteome / Q000032:analysis*
D005656:Fungal Proteins; D020543:Proteome