Alterations of outer membrane proteins and virulence genes expression in gamma-irradiated Vibrio parahaemolyticus and Vibrio alginolyticus.
Gamma-irradiation technology sterilizes microorganisms and thereby prevents decay and improves the safety and shelf stability of food products. In this study we treated the foodborne pathogens Vibrio parahaemolyticus and Vibrio alginolyticus with gamma-irradiation (0.5 kGy) to evaluate their adaptative response. Outer membrane protein patterns of irradiated bacteria were found altered when analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. These modifications were manifested by the appearance and/or disappearance of bands as well as in the expression level of certain proteins. In addition, we searched for the presence of eight Vibrio cholerae virulence genes, toxR, toxS, toxRS, ctxA, zot, ace, toxT, and virulence pathogenicity island (VPI), in the genome of investigated strains. The expression of toxR, toxS, VPI, and ace genes in gamma-irradiated bacteria, studied by reverse transcriptase polymerase chain reaction, was altered. These variations were manifested by an increase and/or a decrease in the expression level of tested virulence genes.
Ben Abdallah
Fethi
F
Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Monastir, Tunisia. fetyben@yahoo.fr
Bakhrouf
Amina
A
Ayed
Atef
A
Kallel
Héla
H
D000220:Adaptation, Biological / Q000528:radiation effects; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D005514:Food Irradiation; D005720:Gamma Rays; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D044149:Vibrio alginolyticus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000528:radiation effects*; D014734:Vibrio cholerae / Q000235:genetics; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000528:radiation effects*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D037521:Virulence Factors