Chiral separation by a monofunctionalized cyclodextrin derivative: from selector to permethyl-beta-cyclodextrin bonded stationary phase.
Preparation of (6-monoureido-6-monodeoxy) permethylated beta-cyclodextrin bonded chiral stationary phase from permethylated 6-monoamino-6-monodeoxy-beta-cyclodextrin is described. The optimized chiral stationary phase was evaluated by using HPLC separation of racemates of coumarin derivatives. Column characterization was performed by solid-state (13)C, (15)N, (29)Si NMR using cross-polarization at the magic angle spinning. The development process was supported by CE experiments where the complex formation between cyclodextrins and warfarin was investigated. The results demonstrate good enantio-discrimination for coumarin derivatives.
Varga
Gábor
G
ChiroQuest Chiral Technologies Development Ltd., Budapest, Hungary.
Tárkányi
Gábor
G
Németh
Krisztina
K
Iványi
Róbert
R
Jicsinszky
László
L
Toke
Orsolya
O
Visy
Júlia
J
Szente
Lajos
L
Szemán
Julianna
J
Simonyi
Miklós
M
D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009587:Nitrogen Isotopes; D012825:Silicon; D013237:Stereoisomerism; D014859:Warfarin / Q000032:analysis / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D002247:Carbon Isotopes; D003374:Coumarins; D009587:Nitrogen Isotopes; D047392:beta-Cyclodextrins; D014859:Warfarin; D012825:Silicon