[Function of human mutant CD59 molecule on diabetic vascular complication].
OBJECTIVE
To investigate the function of human mutant CD59 in diabetic pathology.


METHODS
The recombinant PALTER-MAX plasmid containing human mutant CD59 cDNA and pcDNA plasmid were cotransfected into CHO cells using cation lipoid mediating method. Highly expressed cells were selected by flow cytometry and Western blot, which were then cultivated in high glucose. The functions of restricting complement membrane attack complex formation of glycated HMCD59 and HNCD59 were detected by BCECF release test. ELISA was used to detect the serum concentration of PDGF-BB and VEGF in 30 patients of typeII diabetes without vascular proliferate complication and 30 typeII diabetes patients with vascular proliferate complication.


RESULTS
The expression rate of HM1CD59, HM2CD59, HNCD59 cells was 53.7%, 54.5% and 59.8%, respectively. BCECF release test indicated the release rate of glycated HMCD59 cells was higher than that of unglycated ones (P<0.01)while no significant differences were found between normal HNCD59 cells and glycated ones. The serum concentration of PDGF-BB and VEGF in patients with typeII diabetes markedly increased (P<0.01).


CONCLUSIONS
The decrease of glycated restricting complement membrane attack complex results in the accelerating of human vascular proliferate complication of diabetes.
Zhang
Li
L
Department of Immunology, Medical College of Qingdao University, Qingdao 266000, China.
Gao
Mei-Hua
MH
D000818:Animals; D000077214:Becaplermin; D018957:CD59 Antigens / Q000235:genetics* / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003925:Diabetic Angiopathies / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006031:Glycosylation; D006801:Humans; D009154:Mutation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism
D018957:CD59 Antigens; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin