Recent developments in the localization of oil body-associated signaling molecules during lipolysis in oilseeds.
Prior to and/or accompanying lipolytic degradation of triacylglycerols (TAGs) during seed germination in oilseeds, certain enzymatic and non-enzymatic signaling molecules are expressed on the oil body membranes. These include certain proteases, lipoxygenase, phospholipase A(2) and lipase. Although enough biochemical investigations have demonstrated their activities, recent developments in the in situ localization of these signaling molecules in germinating oilseeds, have enhanced our understanding in this field. This is evident from the temporal and spatial changes observed in the expression pattern of some of these molecules. Present review aims at providing an up-to-date account of these recent developments in the author's and other laboratories, which are largely based on fluorescence microscopic and confocal laser scanning microscopic (CLSM) imaging of the molecular changes using specific fluorescent probes. A model for the molecular events associated with oil body mobilization is also being presented.
Bhatla
Satish C
SC
Department of Botany, University of Delhi, Delhi, India. bhatlasc@gmail.com
Vandana
Shweta
S
Kaushik
Vibha
V
D003546:Cysteine Endopeptidases / Q000378:metabolism; D005456:Fluorescent Dyes; D018525:Germination; D008066:Lipolysis; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D054467:Phospholipases A2 / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D015398:Signal Transduction; D014280:Triglycerides / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
D005456:Fluorescent Dyes; D010938:Plant Oils; D014280:Triglycerides; D054467:Phospholipases A2; D003546:Cysteine Endopeptidases