Brain and blood microRNA expression profiling of ischemic stroke, intracerebral hemorrhage, and kainate seizures.
MicroRNAs (miRNAs) regulate gene expression and have a critical role in many biologic and pathologic processes. We hypothesized that miRNA expression profiles in injured brain (hippocampus) would show common as well as unique profiles when compared with those of blood. Adult, untouched, control rats were compared with rats with sham surgeries, ischemic strokes, brain hemorrhage (lysed blood, fresh blood, or thrombin), and kainate-induced seizures. Brain and whole-blood miRNA expression profiles were assessed 24 h later using TaqMan rodent miRNA arrays. MicroRNA response profiles were different for each condition. Many miRNAs changed more than 1.5-fold in brain and blood after each experimental manipulation, and several miRNAs were upregulated or downregulated in both brain and blood after a given injury. A few miRNAs (e.g., miR-298, miR-155, and miR-362-3p) were upregulated or downregulated more than twofold in both brain and blood after several different injuries. The results show the possible use of blood miRNAs as biomarkers for brain injury; that selected blood miRNAs may correlate with miRNA changes in the brain; and that many of the mRNAs, previously shown to be regulated in brain and blood after brain injury, are likely accounted for by changes in miRNA expression.
Liu
Da-Zhi
DZ
Department of Neurology and the M.I.N.D. Institute, University of California at Davis Medical Center, Sacramento, California 95817, USA. dzliu@ucdavis.edu
Tian
Yingfang
Y
Ander
Bradley P
BP
Xu
Huichun
H
Stamova
Boryana S
BS
Zhan
Xinhua
X
Turner
Renee J
RJ
Jickling
Glen
G
Sharp
Frank R
FR
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists; D007608:Kainic Acid; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D020521:Stroke / Q000378:metabolism*; D015854:Up-Regulation
D018691:Excitatory Amino Acid Antagonists; D035683:MicroRNAs; D007608:Kainic Acid