title: A novel glucosyltransferase involved in O-antigen modification of Shigella flexneri serotype 1c.
abstract: The O antigen of serotype 1c differs from the unmodified O antigen of serotype Y by the addition of a disaccharide (two glucosyl groups) to the tetrasaccharide repeating unit. It was shown here that addition of the first glucosyl group is mediated by the previously characterized gtrI cluster, which is found within a cryptic prophage at the proA locus in the bacterial chromosome. Transposon mutagenesis was performed to disrupt the gene responsible for addition of the second glucosyl group, causing reversion to serotype 1a. Colony immunoblotting was used to identify the desired revertants, and subsequent sequencing, cloning, and functional expression successfully identified the gene encoding serotype 1c-specific O-antigen modification. This gene (designated gtrIC) was present as part of a three-gene cluster, similar to other S. flexneri glucosyltransferase genes. Relative to the other S. flexneri gtr clusters, the gtrIC cluster is more distantly related and appears to have arrived in S. flexneri from outside the species. Analysis of surrounding sequence suggests that the gtrIC cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events.
authors.affiliation: Biochemistry and Molecular Biology, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Stagg
authors.forename: Swee-Seong
authors.initials: SS
authors.lastname: Tang
authors.forename: Nils I A
authors.initials: NI
authors.lastname: Carlin
authors.forename: Kaisar A
authors.initials: KA
authors.lastname: Talukder
authors.forename: Phung D
authors.initials: PD
authors.lastname: Cam
authors.forename: Naresh K
authors.initials: NK
authors.lastname: Verma
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D005964:Glucosyltransferases / Q000378:metabolism*; D005810:Multigene Family; D019081:O Antigens / Q000378:metabolism*; D010802:Phylogeny; D012763:Shigella flexneri / Q000145:classification / Q000201:enzymology* / Q000235:genetics
chemical_list: D019081:O Antigens; D005964:Glucosyltransferases