title: Regulation of the interaction between protein kinase C-related protein kinase 2 (PRK2) and its upstream kinase, 3-phosphoinositide-dependent protein kinase 1 (PDK1).
abstract: The members of the AGC kinase family frequently exhibit three conserved phosphorylation sites: the activation loop, the hydrophobic motif (HM), and the zipper (Z)/turn-motif (TM) phosphorylation site. 3-Phosphoinositide-dependent protein kinase 1 (PDK1) phosphorylates the activation loop of numerous AGC kinases, including the protein kinase C-related protein kinases (PRKs). Here we studied the docking interaction between PDK1 and PRK2 and analyzed the mechanisms that regulate this interaction. In vivo labeling of recombinant PRK2 by (32)P(i) revealed phosphorylation at two sites, the activation loop and the Z/TM in the C-terminal extension. We provide evidence that phosphorylation of the Z/TM site of PRK2 inhibits its interaction with PDK1. Our studies further provide a mechanistic model to explain different steps in the docking interaction and regulation. Interestingly, we found that the mechanism that negatively regulates the docking interaction of PRK2 to the upstream kinase PDK1 is directly linked to the activation mechanism of PRK2 itself. Finally, our results indicate that the mechanisms underlying the regulation of the interaction between PRK2 and PDK1 are specific for PRK2 and do not apply for other AGC kinases.
authors.affiliation: Department of Internal Medicine I, Research Group PhosphoSites, Johann Wolfgang Goethe University Hospital, D-60590 Frankfurt, Germany.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Dettori
authors.forename: Silvina
authors.initials: S
authors.lastname: Sonzogni
authors.forename: Lucas
authors.initials: L
authors.lastname: Meyer
authors.forename: Laura A
authors.initials: LA
authors.lastname: Lopez-Garcia
authors.forename: Nick A
authors.initials: NA
authors.lastname: Morrice
authors.forename: Stefan
authors.initials: S
authors.lastname: Zeuzem
authors.forename: Matthias
authors.initials: M
authors.lastname: Engel
authors.forename: Albrecht
authors.initials: A
authors.lastname: Piiper
authors.forename: Sonja
authors.initials: S
authors.lastname: Neimanis
authors.forename: Morten
authors.initials: M
authors.lastname: Frödin
authors.forename: Ricardo M
authors.initials: RM
authors.lastname: Biondi
mesh_terms: D064413:3-Phosphoinositide-Dependent Protein Kinases; D001665:Binding Sites; D002460:Cell Line; D006801:Humans; D008958:Models, Molecular; D010766:Phosphorylation; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: C061570:protein kinase N; D064413:3-Phosphoinositide-Dependent Protein Kinases; C578562:PDPK1 protein, human; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C