Crystallization and preliminary structural studies of champedak galactose-binding lectin.
Galactose-binding lectin from champedak (Artocarpus integer) consists of two chains: alpha and beta (133 and 21 amino acids, respectively). It has been shown to recognize and bind to carbohydrates involved in IgA and C1 inhibitor molecules. The protein was purified and crystallized at 293 K. Crystals were observed in two space groups, P2(1) and P2(1)2(1)2, and diffracted to 1.65 and 2.6 A, respectively.
Gabrielsen
Mads
M
Division of Molecular and Cellular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland. m.gabrielsen@bio.gla.ac.uk
Riboldi-Tunnicliffe
Alan
A
Abdul-Rahman
Puteri Shafinaz
PS
Mohamed
Emida
E
Ibrahim
Wan Izlina Wan
WI
Hashim
Onn Haji
OH
Isaacs
Neil W
NW
Cogdell
Richard J
RJ
D031622:Artocarpus / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D037161:Galectins / Q000737:chemistry*
D037161:Galectins