Cloning, expression, purification, crystallization and preliminary X-ray analysis of Mycoplasma genitalium protein MG289.
Mycoplasma genitalium is a human pathogen that is associated with nongonococcal urethritis in men and cervicitis in women. The cloning, expression, purification and crystallization of the protein MG289 from M. genitalium strain G37 are reported here. Crystals of MG289 diffracted X-rays to 2.8 A resolution. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 49.7, b = 90.9, c = 176.1 A. The diffraction data after processing had an overall R(merge) of 8.7%. The crystal structure of Cypl, the ortholog of MG289 from M. hyorhinis, has recently been determined, providing a reasonable phasing model; molecular replacement is currently under way.
Sippel
Katherine H
KH
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA.
Boehlein
Susan K
SK
Sakai
Yoshihisa
Y
Quirit
Jeanne G
JG
Agbandje-McKenna
Mavis
M
Rosser
Charles J
CJ
McKenna
Robert
R
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D045704:Mycoplasma genitalium / Q000737:chemistry*
D001426:Bacterial Proteins