title: Molecular variability and genetic structure of the population of soybean mosaic virus based on the analysis of complete genome sequences.
abstract: The complete genomes of 30 Soybean mosaic virus (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutionary constraints and that there was no significant genetic differentiation between East Asian and North American populations of SMV. Phylogenetic analyses revealed a significant correlation between phylogeny of the cylindrical inclusion (CI) gene of SMV and SMV resistance gene 3 (Rsv3)-relating pathogenicity of SMV, suggesting CI might be a pathogenic determinant in Rsv3-mediated disease response. Interestingly, recombination analyses identified 19 'clear' recombination events in the SMV population. Furthermore, as several resistance-breaking strains were identified as recombinants, it appears that recombination might contribute to overcome host resistance in SMV-soybean pathosystem. Our finding suggests that recombination as well as mutation is an important evolutionary process in the genetic diversification of SMV population.
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.forename: Kazusato
authors.initials: K
authors.lastname: Ohshima
authors.forename: Hyeok-Geun
authors.initials: HG
authors.lastname: Lee
authors.forename: Moonil
authors.initials: M
authors.lastname: Son
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
authors.forename: Su-Heon
authors.initials: SH
authors.lastname: Lee
authors.forename: Seong-Han
authors.initials: SH
authors.lastname: Sohn
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D016000:Cluster Analysis; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D007723:Korea; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013025:Glycine max / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D014764:Viral Proteins; D037521:Virulence Factors