A two-photon fluorescent probe for near-membrane calcium ions in live cells and tissues.
A two-photon fluorescent probe (ACaL) is reported that can be excited by 780 nm fs pulses, shows high photostability and negligible toxicity, and can visualize near-membrane Ca2+ in live cells and deep inside live tissues by two-photon microscopy.
Mohan
Palathurai Subramaniam
PS
Department of Chemistry, Bharathiar University, Coimbatore-641046, India.
Lim
Chang Su
CS
Tian
Yu Shun
YS
Roh
Won Young
WY
Lee
Jun Han
JH
Cho
Bong Rae
BR
D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002470:Cell Survival; D002614:Chelating Agents / Q000737:chemistry / Q000378:metabolism; D018891:Dentate Gyrus / Q000378:metabolism; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D007477:Ions / Q000032:analysis* / Q000737:chemistry; D007848:Laurates / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology; D051381:Rats; D013050:Spectrometry, Fluorescence; D019284:Thapsigargin / Q000494:pharmacology
D002614:Chelating Agents; D005456:Fluorescent Dyes; D007477:Ions; D007848:Laurates; D008081:Liposomes; D010281:Parathyroid Hormone; D004533:Egtazic Acid; D019284:Thapsigargin; D015081:2-Naphthylamine; D002118:Calcium; C065580:laurdan