title: Adenosine mediates transforming growth factor-beta 1 release in kidney glomeruli of diabetic rats.
abstract: Up regulation of the transforming growth factor-beta 1 (TGF-beta1) axis has been recognized as a pathogenic event for progression of glomerulosclerosis in diabetic nephropathy. We demonstrate that glomeruli isolated from diabetic rats accumulate up to sixfold more extracellular adenosine than normal rats. Both decreased nucleoside uptake activity by the equilibrative nucleoside transporter 1 and increased AMP hydrolysis contribute to raise extracellular adenosine. Ex vivo assays indicate that activation of the low affinity adenosine A2B receptor subtype (A2BAR) mediates TGF-beta1 release from glomeruli of diabetic rats, a pathogenic event that could support progression of glomerulopathy when the bioavailability of adenosine is increased.
authors.affiliation: Laboratorio Patología Molecular, Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: H
authors.initials: H
authors.lastname: Roa
authors.forename: C
authors.initials: C
authors.lastname: Gajardo
authors.forename: E
authors.initials: E
authors.lastname: Troncoso
authors.forename: V
authors.initials: V
authors.lastname: Fuentealba
authors.forename: C
authors.initials: C
authors.lastname: Escudero
authors.forename: A
authors.initials: A
authors.lastname: Yáñez
authors.forename: L
authors.initials: L
authors.lastname: Sobrevia
authors.forename: M
authors.initials: M
authors.lastname: Pastor-Anglada
authors.forename: C
authors.initials: C
authors.lastname: Quezada
authors.forename: R
authors.initials: R
authors.lastname: San Martin
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D001682:Biological Availability; D003928:Diabetic Nephropathies / Q000378:metabolism*; D033721:Equilibrative Nucleoside Transporter 1 / Q000378:metabolism; D006868:Hydrolysis; D007678:Kidney Glomerulus / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D043704:Receptor, Adenosine A2B / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D033721:Equilibrative Nucleoside Transporter 1; D043704:Receptor, Adenosine A2B; D016212:Transforming Growth Factor beta; D000249:Adenosine Monophosphate; D000241:Adenosine