Cloning and characterization of the promoter of the 9-cis-epoxycarotenoid dioxygenase gene in Arachis hypogaea L.
We cloned the promoter of the 9-cis-epoxycarotenoid dioxygenase gene from Arachis hypogaea L. beta-Glucuronidase (GUS) histochemical staining and GUS activity assay indicated that the activity of the promoter was exhibited predominantly in the leaves and enhanced by water and NaCl stresses, and by application of abscisic acid (ABA) and salicylic acid (SA) in transgenic Arabidopsis. Moreover, two novel ABRE-like (abscisic acid response element) elements were identified in the promoter region.
Liang
Jianhua
J
College of Life Sciences, South China Normal University, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou, 510631, People's Republic of China.
Yang
Lixia
L
Chen
Xiong
X
Li
Ling
L
Guo
Dongliang
D
Li
Haihang
H
Zhang
Biyu
B
D010367:Arachis / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018744:DNA, Plant; D049308:Dioxygenases; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D010940:Plant Proteins; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; D010105:Oxygenases; D049308:Dioxygenases; C109043:9-cis-epoxy-carotenoid dioxygenase; D005966:Glucuronidase