title: Crystallization and preliminary X-ray crystallographic analysis of PhoK, an extracellular alkaline phosphatase from Sphingomonas sp. BSAR-1.
abstract: Alkaline phosphatases (APs) are widely distributed from microbes to humans and are involved in several important biological processes such as phosphate nutrition, signal transduction and pathogenesis. Alkaline phosphatases are also useful in various industrial applications and in recombinant DNA technology. A new AP enzyme from Sphingomonas sp. strain BSAR-1, termed PhoK, has been shown to be useful in uranium bioprecipitation. PhoK was expressed, purified and crystallized. The crystals belonged to space group P4(3)2(1)2 or P4(1)2(1)2, with unit-cell parameters a = b = 87.37, c = 168.16 A, and contained one enzyme molecule in the asymmetric unit. Native diffraction data have been collected to 1.95 A resolution at the ESRF.
authors.affiliation: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
authors.forename: Kayzad S
authors.initials: KS
authors.lastname: Nilgiriwala
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Bihani
authors.forename: Amit
authors.initials: A
authors.lastname: Das
authors.forename: Vishal
authors.initials: V
authors.lastname: Prashar
authors.forename: Mukesh
authors.initials: M
authors.lastname: Kumar
authors.forename: Jean Luc
authors.initials: JL
authors.lastname: Ferrer
authors.forename: Shree Kumar
authors.initials: SK
authors.lastname: Apte
authors.forename: M V
authors.initials: MV
authors.lastname: Hosur
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005110:Extracellular Space / Q000201:enzymology*; D020578:Sphingomonas / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D000469:Alkaline Phosphatase