title: Dynamic culture of osteogenic cells in biomimetically coated poly(caprolactone) nanofibre mesh constructs.
abstract: In our previous work, biomimetic calcium phosphate-coated poly(caprolactone) nanofibre meshes (BCP-NMs) were demonstrated to be more effective for supporting cell attachment and proliferation under static conditions, when compared with poly(caprolactone) nanofibre meshes (PCL-NMs). In many applications, in vitro cultivation of constructs using bioreactors that support efficient nutrition of cells has appeared as an important step toward the development of functional grafts. This work aimed at studying the effects of dynamic culture conditions and biomimetic coating on bone cells grown on the nanofibre meshes. BCP-NM and PCL-NM were seeded with osteoblast-like cells (MG63--human osteosarcoma-derived cell line). The cell-seeded constructs were cultured within a rotating bioreactor that simulated microgravity, at a fixed rotating speed, for different time periods, and then characterized. Cell morphology, viability, and phenotype were assessed. PCL-NM constructs presented a higher number of dead cells than BCP-NM constructs. Under dynamic conditions, the production of proteins associated with the extracellular matrix of bone was higher on BCP-NM constructs than in the PCL-NM ones, which indicates that coated samples may provide cells with a better environment for tissue growth. It is suggested that improved mass transfer in the bioreactor in combination with the appropriate substrate were decisive factors for this highly positive outcome for generating bone.
authors.affiliation: 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.
authors.forename: Jose V
authors.initials: JV
authors.lastname: Araujo
authors.forename: Cassilda
authors.initials: C
authors.lastname: Cunha-Reis
authors.forename: Tommaso
authors.initials: T
authors.lastname: Rada
authors.forename: Marta Alves
authors.initials: MA
authors.lastname: da Silva
authors.forename: Manuela E
authors.initials: ME
authors.lastname: Gomes
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Nureddin
authors.initials: N
authors.lastname: Ashammakhi
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: El-Haj
authors.forename: Nuno M
authors.initials: NM
authors.lastname: Neves
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D040761:Biomimetic Materials / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D004247:DNA