title: [Cloning and bioinformatics analysis of SLA-DR genes in Hunan Daweizi pigs].
abstract: OBJECTIVE
  To evaluate the potential of Daweizi pigs as xenotransplantation dnors from pigs to humans by analyzing the characteristics of SLA-DR genes in Hunan Daweizi pigs.
  
  
  METHODS
  SLA-DRA and SLA-DRB genes were amplified by RT-PCR, cloned into pUCm-T vectors, sequenced and analyzed through BLAST in NCBI and related software in ExPASY.
  
  
  RESULTS
  The SLA-DRA and SLA-DRB genes were 1 177 bp and 909 bp nucleotides in length, which contain opening reading frame (ORF) and encode 252 and 266 amino acids respectively. Comparing the SLA-DRA and SLA-DRB genes with their counterpart sequences of human, the homologies of amino acid sequences were 82% and 73% respectively. The amino acids in SLA DR alpha chain of Daweizi pigs from position 124 to 136, which bind to human CD4, showed only two differences with HLA DRA: a lle-Val change at position 127 and a Ser-Thr change at position 136. The amino acids in SLA DR beta chain of Daweizi pigs from position 134 to 148, which bind to human CD4, were identical with HLA-DRB. Further comparison with SLA sequences published in GenBank indicated that SLA-DRB gene found in Daweizi pigs has polymorphism while the homology of SLA-DRA gene is up to 100%.
  
  
  CONCLUSIONS
  The cloned SLA-DRA and SLA-DRB in Hunan Daweizi pigs has high polymorphism with HLA-DRA and HLA-DRB in Human, indicates that Daweizi pigs have some advantages as xenotransplantation dnors from pigs to humans.
authors.affiliation: Cell Transplantation & Gene Therapy Center, Third Xiangya Hospital of Central South University, Changsha 410013, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Xing
authors.forename: Li-qun
authors.initials: LQ
authors.lastname: Xue
authors.forename: Sheng-qiang
authors.initials: SQ
authors.lastname: Huang
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D003001:Cloning, Molecular; D019295:Computational Biology; D006684:HLA-DR Antigens / Q000737:chemistry / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D013552:Swine / Q000145:classification / Q000235:genetics* / Q000276:immunology
chemical_list: D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen