Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods.
The aim of this study was to compare two nucleic acid extraction methods for the recovery of enteric viruses from activated sludge. Test samples were inoculated with human adenovirus (AdV), hepatitis A virus (HAV), poliovirus (PV) and rotavirus (RV) and were then processed by an adsorption-elution-precipitation method. Two extraction methods were used: an organic solvent-based method and a silica method. The organic-based method was able to recoup 20% of the AdV, 90% of the RV and 100% of both the PV and HAV from seeded samples. The silica method was able to recoup 1.8% of the AdV and 90% of the RV. These results indicate that the organic-based method is more suitable for detecting viruses in sewage sludge.
Schlindwein
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Departamentode Microbiologia e Parasitologia, Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brasil.
Simões
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Barardi
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D000260:Adenoviruses, Human / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D030041:Hepatitis A virus / Q000302:isolation & purification; D017955:Poliovirus / Q000302:isolation & purification; D012328:RNA Viruses / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification; D012722:Sewage / Q000821:virology*; D014871:Water Microbiology
D004279:DNA, Viral; D012367:RNA, Viral; D012722:Sewage