Changes of resveratrol and antioxidant enzymes during UV-induced plant defense response in peanut seedlings.
Plants have evolved mechanisms to avoid and repair UV radiation damage, and the free radicals caused by UV tend to be involved in the induction of antioxidant defense systems. In this study, changes in resveratrol and antioxidant enzymes were investigated in relation to UV damage in peanut seedlings. Accumulation of endogenous resveratrol and stilbene synthase mRNA occurred rapidly and significantly in response to UV-C irradiation. Applying resveratrol before UV-C irradiation mitigated rusty spots and wilting of peanut leaves, and inhibition of resveratrol by applying 3,4-methylenedioxycinnamic acid worsened UV-C damage, an effect that was found to be concentration dependent. Correspondingly, the effect of resveratrol on malondialdehyde was similar to changes in the apparent morphology of seedling leaves. Changes in H(2)O(2), O(2)(-), and antioxidant enzymes showed some similarities after either UV-C irradiation or resveratrol treatment. Activities of superoxide dismutases, glutathione reductase, and catalase were more than 2-fold higher during the first 1h after treatments. Ascorbate peroxidase activity increased to more than 3-fold higher 24h after irradiation, whereas it was more than 2-fold higher 8h after resveratrol treatment. Activities of dehydroascorbate reductase and monodehydroascorbate reductase increased by 40% during 8-24h after treatments. Consequently, we proposed that changes in endogenous resveratrol and in antioxidant enzymes may have been involved in oxidative stress induced by UV-C exposure in peanut seedlings.
Tang
Ke
K
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Zhan
Ji-Cheng
JC
Yang
Hao-Ru
HR
Huang
Wei-Dong
WD
D000975:Antioxidants / Q000378:metabolism*; D010367:Arachis / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism* / Q000528:radiation effects; D002374:Catalase / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D005980:Glutathione Reductase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D000077185:Resveratrol; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism* / Q000528:radiation effects; D013267:Stilbenes / Q000378:metabolism / Q000493:pharmacokinetics; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
D000975:Antioxidants; D013267:Stilbenes; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D000077185:Resveratrol