Gene expression in chorionic villous samples at 11 weeks of gestation in women who develop preeclampsia later in pregnancy: implications for screening.
OBJECTIVE
To determine the gene expression profile in chorionic villous samples (CVS) of women destined to develop preeclampsia.


METHODS
cDNA microarray technology was employed. Ten singleton fetuses of women who subsequently developed preeclampsia where compared with a pool of 50 controls. The mRNA expression of some of the genes previously found to be up- or down-regulated were validated by RT-PCR in peripheral blood from 23 pregnant women at term affected with preeclampsia and 23 controls.


RESULTS
Altered expression was found among several genes including those involved in invasion of human trophoblasts (Titin), in inflammatory stress (Lactotransferrin), endothelial aberration (Claudin 6), angiogenesis (Vasohibin 1), blood pressure control (Adducin 1). Also the peripheral blood from preeclampsia patients showed significant differences for all the genes studied.


CONCLUSIONS
CVS show an aberrant gene profile prior to preeclampsia onset that may be predictive of the disease.
Farina
Antonio
A
Department of Histology, Embryology and Applied Biology, Division of Prenatal Medicine, Via Belmeloro 8, 40126 Bologna, Italy. antonio.farina@unibo.it
Morano
Danila
D
Arcelli
Diego
D
De Sanctis
Paola
P
Sekizawa
Akihiko
A
Purwosunu
Yuditiya
Y
Zucchini
Cinzia
C
Simonazzi
Giuliana
G
Okai
Takashi
T
Rizzo
Nicola
N
D000328:Adult; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000378:metabolism* / Q000473:pathology; D015193:Chorionic Villi Sampling; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005820:Genetic Testing / Q000379:methods; D005865:Gestational Age; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011225:Pre-Eclampsia / Q000175:diagnosis* / Q000235:genetics; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000235:genetics* / Q000378:metabolism; D011263:Pregnancy Trimester, Third; D011379:Prognosis