[Effect of TSP-2 antibody against a single epitope of mouse Toll-like receptor 2 extracellular domain on zymosan A-induced peritonitis in mice].
OBJECTIVE
To observe the effect of the antibody TSP-2 against a single epitope of mouse Toll-like receptor 2 extracellular domain (mTLR2ECD) on the inflammation in mice with zymosan A-induced peritonitis.


METHODS
In mice with peritonitis induced by intraperitoneal injection of zymosan A, pretreatments with PBS, normal rabbit IgG and TSP-2 antibody at two different doses (2.5 and 5.0 mg/kg) were administered via the tail vein. Six hours after intraperitoneal injection of zymosan A, Evans blue was injected through the tail vein, and the frequency of writhing of the mice within 20 min were recorded. The mice were then sacrificed for peritoneal lavage, and the lavage fluid was collected to assess the exudation of Evans blue in the supernatant. The peritoneal leukocyte count, mast cell degranulation and release of such inflammatory mediators as platelet activating factor (PAF) and tumor necrosis factor-alpha (TNFalpha) in the lavage fluid were observed by cell counting, specific cell staining, immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).


RESULTS
Compared with PBS or rabbit IgG groups, TSP-2 treatment resulted in significantly reduced writhing response of the mice and lowered Evans blue exudation and leukocyte count in the peritoneal lavage, with also decreased degranulation of the mast cells induced by C48/80.


CONCLUSIONS
TSP-2 antibody against a single epitope of mTLR2ECD inhibits the inflammatory response in mice with zymosan A-induced peritonitis.
Yang
Cui-lan
CL
Department of Cell Biology, Southern Medical University, Guangzhou 510515, China. ycllb@fimmu.com
Zhao
Wen-zhong
WZ
Huang
En-ping
EP
Luo
Shen-qiu
SQ
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001522:Behavior, Animal; D000939:Epitopes / Q000276:immunology*; D005110:Extracellular Space; D005260:Female; D007958:Leukocyte Count; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D010533:Peritoneal Lavage; D010538:Peritonitis / Q000139:chemically induced* / Q000276:immunology*; D017434:Protein Structure, Tertiary; D051195:Toll-Like Receptor 2 / Q000737:chemistry* / Q000276:immunology*; D015054:Zymosan / Q000494:pharmacology*
D000906:Antibodies; D000939:Epitopes; D051195:Toll-Like Receptor 2; D015054:Zymosan