title: Modified live marker vaccine candidate CP7_E2alf provides early onset of protection against lethal challenge infection with classical swine fever virus after both intramuscular and oral immunization.
abstract: Due to the vast economic consequences of classical swine fever (CSF) outbreaks, emergency vaccination plans are under discussion in European Union Member States. However, animals vaccinated with the conventional C-strain vaccine are subject to trade restrictions. To ease these restrictions, potent marker vaccines are required. One promising candidate is the chimeric pestivirus CP7_E2alf. For emergency vaccination in a CSF outbreak scenario, early onset of immunity is required. Here, the studies performed with a CP7_E2alf virus stock produced under good manufacturing conditions (GMP) are reported. In challenge experiments, CP7_E2alf induced full clinical protection 1 week after intramuscular vaccination, and 2 weeks after oral immunization. Furthermore, even after application of diluted vaccine preparations complete protection could be achieved if challenge infection was carried out 4 weeks after vaccination. In conclusion, GMP-produced CP7_E2alf proved to be a suitable marker vaccine candidate - also for emergency vaccination - both after intramuscular and oral application.
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany.
authors.forename: Immanuel
authors.initials: I
authors.lastname: Leifer
authors.forename: Elke
authors.initials: E
authors.lastname: Lange
authors.forename: Ilona
authors.initials: I
authors.lastname: Reimann
authors.forename: Sandra
authors.initials: S
authors.lastname: Blome
authors.forename: Sandra
authors.initials: S
authors.lastname: Juanola
authors.forename: Joan Plana
authors.initials: JP
authors.lastname: Duran
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D009154:Mutation; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA; D013552:Swine; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D014765:Viral Vaccines