title: Hospicells (ascites-derived stromal cells) promote tumorigenicity and angiogenesis.
abstract: The microenvironment is known to play a dominant role in cancer progression. Cells closely associated with tumoral cells, named hospicells, have been recently isolated from the ascites of ovarian cancer patients. Whilst these cells present no specific markers from known cell lineages, they do share some homology with bone marrow-derived or adipose tissue-derived human mesenchymal stem cells (CD9, CD10, CD29, CD146, CD166, HLA-1). We studied the role of hospicells in ovarian carcinoma progression. In vitro, these cells had no effect on the growth of human ovarian carcinoma cell lines OVCAR-3, SKOV-1 and IGROV-1. In vivo, their co-injection with adenocarcinoma cells enhanced tumor growth whatever the tumor model used (subcutaneous and intraperitoneally established xenografts in athymic mice). In addition, their injection increased the development of ascites in tumor-bearing mice. Fluorescent macroscopy revealed an association between hospicells and ovarian adenocarcinoma cells within the tumor mass. Tumors obtained by coinjection of hospicells and human ovarian adenocarcinoma cells presented an increased microvascularization indicating that the hospicells could promote tumorigenicity of ovarian tumor cells in vivovia their action on angiogenesis. This effect on angiogenesis could be attributed to the increased HIF1alpha and VEGF expression associated with the presence of the hospicells. Collectively, these data indicate a role for these ascite-derived stromal cells in promoting tumor growth by increasing angiogenesis.
authors.affiliation: EA3035, Institut Claudius Regaud, University Toulouse III, Faculté des Sciences Pharmaceutiques, Toulouse, F - 31062, France.
authors.forename: Marlene
authors.initials: M
authors.lastname: Pasquet
authors.forename: Muriel
authors.initials: M
authors.lastname: Golzio
authors.forename: Eliane
authors.initials: E
authors.lastname: Mery
authors.forename: Arash
authors.initials: A
authors.lastname: Rafii
authors.forename: Nadia
authors.initials: N
authors.lastname: Benabbou
authors.forename: Pezhman
authors.initials: P
authors.lastname: Mirshahi
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hennebelle
authors.forename: Philippe
authors.initials: P
authors.lastname: Bourin
authors.forename: Ben
authors.initials: B
authors.lastname: Allal
authors.forename: Justin
authors.initials: J
authors.lastname: Teissie
authors.forename: Massoud
authors.initials: M
authors.lastname: Mirshahi
authors.forename: Bettina
authors.initials: B
authors.lastname: Couderc
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D001201:Ascites / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000502:physiology; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000209:etiology*; D009389:Neovascularization, Pathologic / Q000209:etiology*; D010051:Ovarian Neoplasms / Q000473:pathology; D010641:Phenotype; D017154:Stromal Cells / Q000502:physiology*; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A