Severe pandemic (H1N1) 2009 influenza with false negative direct fluorescent antibody assay: case series.
Between May and June of 2009 we observed 4 patients that presented with severe influenza-like symptoms and respiratory failure. All cases tested negative for influenza A and B by direct fluorescent antibody assay. Further investigation revealed all cases to be positive for pandemic (H1N1) 2009 influenza virus by real-time RT-PCR. This article includes a description of these cases and the characteristics associated with them.
Kapelusznik
Luciano
L
Division of Infectious Diseases, Mount Sinai School of Medicine, New York, NY 10029, United States. luciano.kapelusznik@mssm.edu
Patel
Rupa
R
Jao
Jennifer
J
Patel
Gopi
G
Daefler
Simon
S
Labombardi
Vincent
V
Calfee
David
D
D000328:Adult; D000368:Aged; D005188:False Negative Reactions; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*