Effect of homologous PRP on proliferation of human periodontally affected osteoblasts. In vitro preliminary study. Report of a case.
OBJECTIVE
The purpose of this in vitro study was to evaluate the effect of two concentrations of homologous platelet-rich plasma (PRP) on the proliferative response of osteoblasts derived from a patient with aggressive periodontitis.


METHODS
8.5 ml of venous blood were taken from 1 healthy and non-smoker volunteer. PRP was prepared following the protocol of Curasan. Osteoblasts were derived from alveolar bone chips obtained from a patient with aggressive periodontitis during conventional periodontal surgery and a clinically healthy person during crown lengthening surgical procedure. Cells were grown in 24-well dishes and on day 2 of quiescence were treated with 1% and 5% (v/v) of PRP. The effect on cell proliferation was estimated by measuring [3H] thymidine incorporation. After 48h of incubation, cells were processed to subject to scintillation counting. Counts per minute were determined for each sample.


RESULTS
The addition of 1% and 5% of PRP provoked a statistical significant (p<0.05) increase in cell growth.


CONCLUSIONS
Data revealed significant enhancement of proliferative response of osteoblasts in the presence of PRP, which might serve as a source of growth factors promoting periodontal repair by modulating cell response and activities.
Markopoulou
C E
CE
Department of Periodontology, School of Dentistry, University of Athens, Greece.
Markopoulos
P
P
Dereka
X E
XE
Pepelassi
E
E
Vrotsos
I A
IA
D000328:Adult; D016301:Alveolar Bone Loss / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D055113:Chronic Periodontitis / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism / Q000494:pharmacology*; D007568:Jaw / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000737:chemistry* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D036341:Intercellular Signaling Peptides and Proteins; D013936:Thymidine