Impact of phenolic substrate and growth temperature on the Arthrobacter chlorophenolicus proteome.
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
Unell
Maria
M
Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07 Uppsala, Sweden.
Abraham
Paul E
PE
Shah
Manesh
M
Zhang
Bing
B
Rückert
Christian
C
VerBerkmoes
Nathan C
NC
Jansson
Janet K
JK
D001173:Arthrobacter / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002733:Chlorophenols / Q000494:pharmacology; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D009596:Nitrophenols / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013696:Temperature
D001426:Bacterial Proteins; D002733:Chlorophenols; D009596:Nitrophenols; D010636:Phenols; D020543:Proteome; C029107:4-chlorophenol; C024836:4-nitrophenol