title: Molecular characterization of a new isolate of Borrelia lusitaniae derived from Apodemus sylvaticus in Portugal.
abstract: A total of 196 small mammals were collected in Portugal and tested for Borrelia burgdorferi sensu lato. Tissue samples were taken from each animal and cultured in Barbour-Stoenner-Kelly (BSK)-II medium. The single strain of spirochete isolated was confirmed as Borrelia lusitaniae by genetic analyses. This is the first report of B. lusitaniae isolated from Apodemus sylvaticus.
authors.affiliation: Center for Vector and Infectious Diseases Research, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal. isabel.carvalho@insa.min-saude.pt
authors.forename: Isabel Lopes
authors.initials: IL
authors.lastname: de Carvalho
authors.forename: Nordin
authors.initials: N
authors.lastname: Zeidner
authors.forename: Amy
authors.initials: A
authors.lastname: Ullmann
authors.forename: Andrias
authors.initials: A
authors.lastname: Hojgaard
authors.forename: Fátima
authors.initials: F
authors.lastname: Amaro
authors.forename: Líbia
authors.initials: L
authors.lastname: Zé-Zé
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Alves
authors.forename: Rita
authors.initials: R
authors.lastname: de Sousa
authors.forename: Joseph
authors.initials: J
authors.lastname: Piesman
authors.forename: Maria Sofia
authors.initials: MS
authors.lastname: Núncio
mesh_terms: D000818:Animals; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000302:isolation & purification*; D004197:Disease Reservoirs; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology; D051189:Murinae / Q000382:microbiology*; D010802:Phylogeny; D011174:Portugal / Q000453:epidemiology