title: Somatic cell nuclear transfer in zebrafish.
abstract: We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.
authors.affiliation: Comparative Medicine and Integrative Biology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.
authors.forename: Kannika
authors.initials: K
authors.lastname: Siripattarapravat
authors.forename: Boonya
authors.initials: B
authors.lastname: Pinmee
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Venta
authors.forename: Chia-Cheng
authors.initials: CC
authors.lastname: Chang
authors.forename: Jose B
authors.initials: JB
authors.lastname: Cibelli
mesh_terms: D000818:Animals; D005818:Genetic Engineering / Q000379:methods*; D006822:Hybrid Cells / Q000637:transplantation*; D053652:Nuclear Transfer Techniques; D015027:Zebrafish / Q000033:anatomy & histology* / Q000235:genetics*