title: Experiments on moving interaction boundaries and their characteristics of focusing and probing of both guest and host target molecules.
abstract: In this paper, the concept of a moving interaction boundary (MIB) is proposed with regard to guest and host molecules. With 2-naphthalene-sulfonate (2-NS) and beta-cyclodextrin (CD) as the model guest and host compounds, respectively, the relevant experiments were carried out on the MIB in capillary electrophoresis (CE). The experiments show that (1) there are a MIB and a complex boundary (CB) if proper guest and host molecules are used; (2) the MIB system has the characteristic of selective focusing and probing of the target 2-NS; (3) the system also has the characteristic of selective probing of the target host molecule beta-CD without UV-absorbance, making the direct UV determination of beta-CD from other CDs possible; (4) interestingly, the focusing of the guest molecule is a kind of leaky-sample stacking rather than a collection of analytes in sample sweeping; (5) the mechanism of MIB-induced separation of target analyte from unwanted ones is similar to but different from that of an affinity chromatography. In addition, the utility of MIB was briefly tested for a real sample of wastewater spiked with 2-NS.
authors.affiliation: Laboratory of Analytical Biochemistry & Bioseparation, Ministry of Education, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, China.
authors.forename: Liuyin
authors.initials: L
authors.lastname: Fan
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Chengxi
authors.initials: C
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
mesh_terms: D001888:Boric Acids / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009282:Naphthalenesulfonates / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D001888:Boric Acids; D009282:Naphthalenesulfonates; D047392:beta-Cyclodextrins; C511690:naphthalene-2-sulfonic acid; C031215:betadex; C032688:boric acid