Influences of flavonoids on erythrocyte membrane and metabolic implication through anionic exchange modulation.
The antioxidative activity of some natural flavonoids was analyzed against the stable free radical 2,2-diphenyl-1-picryhydrazyl. The results indicate that the scavenging power of the tested flavonols is higher than that of the synthetic antioxidants butylated hydroxyanisole and butylated hydroxytoluene; instead, the flavanones show little activity, as indicated by efficient concentration (EC50) values. Flavonoid autoxidation and interaction with Fe2+ and hydrogen peroxide were tested using erythrocyte membranes as a model. The results show that some compounds, like hesperetin, evidence a pro-oxidant activity higher than the ascorbic acid/iron reference system. The compounds with strong oxidative capability do not only influence cellular redox balance but also activate caspase-3, producing lactate dehydrogenase release and enhancing anionic exchange at the level of band 3 protein.
Barreca
Davide
D
Organic and Biological Chemistry Department, University of Messina, Messina, Italy.
Laganà
Giuseppina
G
Tellone
Ester
E
Ficarra
Silvana
S
Leuzzi
Ugo
U
Galtieri
Antonio
A
Bellocco
Ersilia
E
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002083:Butylated Hydroxyanisole / Q000737:chemistry / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000737:chemistry / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D008297:Male; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010851:Picrates; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron