In vivo assessment of macrophage CNS infiltration during disruption of the blood-brain barrier with focused ultrasound: a magnetic resonance imaging study.
Focused ultrasound has been discovered to locally and reversibly increase permeability of the blood-brain barrier (BBB). However, inappropriate sonication of the BBB may cause complications, such as hemorrhage and brain tissue damage. Tissue damage may be controlled by selecting optimal sonication parameters. In this study, we sought to investigate the feasibility of labeling cells with superparamagnetic iron oxide particles to assess the inflammatory response during focused-ultrasound-induced BBB opening. We show that infiltration of phagocytes does not occur using optimal parameters of sonication. Taken together, the results of our study support the usefulness and safety of focused-ultrasound-induced BBB opening for enhancing drug delivery to the brain. These findings may have implications for the optimization of sonication parameters.
Liu
Hao-Li
HL
Department of Electrical Engineering, Chang-Gung University, Taoyuan, Taiwan.
Wai
Yau-Yau
YY
Hsu
Po-Hong
PH
Lyu
Lee-Ang
LA
Wu
Jia-Shin
JS
Shen
Chia-Rui
CR
Chen
Jin-Chung
JC
Yen
Tzu-Chen
TC
Wang
Jiun-Jie
JJ
D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D002138:Calibration; D002490:Central Nervous System / Q000166:cytology* / Q000000981:diagnostic imaging*; D005290:Ferric Compounds; D008264:Macrophages / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D008297:Male; D020556:Neutrophil Infiltration / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014463:Ultrasonography
D005290:Ferric Compounds; C000499:ferric oxide