Production and secretion of adiponectin from 3T3-L1 adipocytes: comparison of antihypertensive drugs.
BACKGROUND
Adiponectin is an important vascular protective adipocytokine that possesses antidiabetic, antiatherogenic, and anti-inflammatory properties. The aim of this study was to evaluate the effect of various antihypertensive drugs on the production and secretion of adiponectin from adipocytes.


METHODS
3T3-L1 adipocytes were incubated for 6 h with increased doses of the following drugs: hydrochlorothiazide, atenolol, losartan, telmisartan, captopril, and nifedipine. Adiponectin levels, as well as adiponectin-mRNA expression, were measured in the medium and cells.


RESULTS
Significant increases of adiponectin were induced by telmisartan: 56% with a dose of 0.1 micromol/l (P < 0.05), 131% with 10 micromol/l (P < 0.05), and 125% with 100 micromol/l (P < 0.01). Losartan (100 micromol/l) also increased adiponectin by 65% (P < 0.05). Conversely, hydrochlorothiazide, 0.1 micromol/l, reduced adiponectin by 37% (P < 0.01). Captopril, atenolol, and nifedipine had no effect on adiponectin. Gene expression of adiponectin correlated with these results: with telmisartan, it increased by 27%, and with hydrochlorothiazide it decreased by 38% (P < 0.05 for both compared to the control).


CONCLUSIONS
In this comparative model, telmisartan, and to a lesser extent, losartan, increased production and secretion of adiponectin from 3T3-L1 adipocytes compared to the other antihypertensive drugs.
Brody
Raphaelle
R
Hypertension Unit, The Chaim Sheba Medical Center, Tel Hashomer, Israel.
Peleg
Edna
E
Grossman
Ehud
E
Sharabi
Yehonatan
Y
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001262:Atenolol / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D006852:Hydrochlorothiazide / Q000494:pharmacology; D019808:Losartan / Q000494:pharmacology; D051379:Mice; D009543:Nifedipine / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D000077333:Telmisartan
D052242:Adiponectin; D000959:Antihypertensive Agents; D001562:Benzimidazoles; D001565:Benzoates; D012333:RNA, Messenger; D006852:Hydrochlorothiazide; D001262:Atenolol; D002216:Captopril; D009543:Nifedipine; D019808:Losartan; D000077333:Telmisartan