title: Contribution of mobile genetic elements to Desulfovibrio vulgaris genome plasticity.
abstract: The genome of Desulfovibrio vulgaris strain DePue, a sulfate-reducing Deltaproteobacterium isolated from heavy metal-impacted lake sediment, was completely sequenced and compared with the type strain D. vulgaris Hildenborough. The two genomes share a high degree of relatedness and synteny, but harbour distinct prophage and signatures of past phage encounters. In addition to a highly variable phage contribution, the genome of strain DePue contains a cluster of open-reading frames not found in strain Hildenborough coding for the production and export of a capsule exopolysaccharide, possibly of relevance to heavy metal resistance. Comparative whole-genome microarray analysis on four additional D. vulgaris strains established greater interstrain variation within regions associated with phage insertion and exopolysaccharide biosynthesis.
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Walker
authors.forename: Sergey
authors.initials: S
authors.lastname: Stolyar
authors.forename: Dylan
authors.initials: D
authors.lastname: Chivian
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pinel
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Gabster
authors.forename: Paramvir S
authors.initials: PS
authors.lastname: Dehal
authors.forename: Zhili
authors.initials: Z
authors.lastname: He
authors.forename: Zamin Koo
authors.initials: ZK
authors.lastname: Yang
authors.forename: Huei-Che B
authors.initials: HC
authors.lastname: Yen
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
authors.forename: Judy D
authors.initials: JD
authors.lastname: Wall
authors.forename: Terry C
authors.initials: TC
authors.lastname: Hazen
authors.forename: Adam P
authors.initials: AP
authors.lastname: Arkin
authors.forename: David A
authors.initials: DA
authors.lastname: Stahl
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D016969:Desulfovibrio vulgaris / Q000145:classification / Q000235:genetics*; D016680:Genome, Bacterial; D044404:Genomic Islands; D020071:Interspersed Repetitive Sequences; D046228:Microarray Analysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial