title: A proteomic view into infection of greyback canegrubs (Dermolepida albohirtum) by Metarhizium anisopliae.
abstract: Metarhizium anisopliae is a naturally occurring cosmopolitan fungus infecting greyback canegrubs (Dermolepida albohirtum). The main molecular factors involved in the complex interactions occurring between the greyback canegrubs and M. anisopliae (FI-1045) were investigated by comparing the proteomes of healthy canegrubs, canegrubs infected with Metarhizium and fungus only. Differentially expressed proteins from the infected canegrubs were subjected to mass spectrometry to search for pathogenicity related proteins. Immune-related proteins of canegrubs identified in this study include cytoskeletal proteins (actin), cell communication proteins, proteases and peptidases. Fungal proteins identified include metalloproteins, acyl-CoA, cyclin proteins and chorismate mutase. Comparative proteome analysis provided a view into the cellular reactions triggered in the canegrub in response to the fungal infection at the onset of biological control.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia. nchikbal@bio.mq.edu.au
authors.forename: Nirupama Shoby
authors.initials: NS
authors.lastname: Manalil
authors.forename: Valentino S
authors.initials: VS
authors.lastname: Junior Te'o
authors.forename: Kathy
authors.initials: K
authors.lastname: Braithwaite
authors.forename: Stevens
authors.initials: S
authors.lastname: Brumbley
authors.forename: Peter
authors.initials: P
authors.lastname: Samson
authors.forename: K M Helena
authors.initials: KM
authors.lastname: Nevalainen
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000254:growth & development / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D019476:Insect Proteins / Q000378:metabolism; D052981:Metarhizium / Q000502:physiology*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019476:Insect Proteins; D020543:Proteome