Development of glucose sensor using two-photon adsorbed  photopolymerization.
A novel glucose sensor was constructed, and its analytical potential examined. A chip-type three-electrode system for use in a flow-type electrochemical glucose sensor was fabricated using a UV lithography technique on a glass slide. An Ag/AgCl reference electrode was made by electroplating silver onto a Pt electrode and dipping in a saturated KCl solution for 30 min. In addition, a glucose-sensing electrode was fabricated using a two-photon adsorbed photopolymerization technique with a photo-reactive resin containing a glucose oxidase enzyme, ferrocene mediator, non-ionic surfactant, and carbon nanotubes. The cyclic voltammetry of the potassium ferrocyanide in the Pt sensor system showed a stable electrode condition. The response of the modified Pt sensor confirms the feasibility of using a two-photon adsorbed photopolymerization technique for the easy fabrication of functional biosensors.
Kim
Jong Min
JM
Department of Chemical Engineering, Dong-A University, 840 Hadan-dong, Saha-Gu, Pusan 604-714, Korea.
Park
Jung-Jin
JJ
Lee
Haeng-Ja
HJ
Kim
Woo-Sik
WS
Muramatsu
Hiroshi
H
Chang
Sang-Mok
SM
D001786:Blood Glucose / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods*; D005292:Ferricyanides / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods; D017785:Photons; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
D001786:Blood Glucose; D005292:Ferricyanides; D037742:Nanotubes, Carbon; D018030:Silver Compounds; D012834:Silver; D005949:Glucose Oxidase; C037548:silver chloride; C028033:potassium ferricyanide