Slackia equolifaciens sp. nov., a human intestinal bacterium capable of producing equol.
An equol-producing bacterium, strain DZE(T), which was isolated from human faeces, was characterized by morphological, biochemical and molecular methods. The isolate was Gram-positive, obligately anaerobic, non-spore-forming, asaccharolytic and rod-shaped. 16S rRNA gene sequence analysis showed 92.8, 91.0, 91.1 and 90.6% similarities with Slackia faecicanis, Slackia exigua, Slackia heliotrinireducens and Slackia isoflavoniconvertens, respectively. Based on these data, we propose a novel species of the genus Slackia, Slackia equolifaciens sp. nov. The major cellular fatty acids are C14:0, C18:1omega9c and C18:1omega9c DMA (dimethyl acetal). The DNA G+C content of the strain is 60.8 mol%. The type strain of S. equolifaciens sp. nov. is DZET (=JCM 16059T =CCUG 58231T).
Jin
Jong-Sik
JS
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.|Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|Benno Laboratory, Center for Intellectual Property Strategies, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Kitahara
Maki
M
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Sakamoto
Mitsuo
M
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Hattori
Masao
M
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Benno
Yoshimi
Y
Benno Laboratory, Center for Intellectual Property Strategies, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D060754:Equol; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007529:Isoflavones / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D007529:Isoflavones; D012336:RNA, Ribosomal, 16S; D060754:Equol