Biological decolorization of dye solution containing malachite green by Pandoraea pulmonicola YC32 using a batch and continuous system.
In our study, we have isolated a relatively newly identified bacteria species, Pandoraea pulmonicola YC32, and first assessed its capability to treat malachite green (MG). The effects of various factors on decolorization efficiency were investigated in a batch system. The decolorization efficiency was found to be optimal within a pH of 7-10 and it increased, with increasing initial MG concentration up to 100 mg/l. The relationship between the decolorization rate and MG concentration agreed with Lineweaver-Burk equation. The apparent kinetic parameters, R(MG,max) and K(m), were 6.23 mg-MG/g-cell/h and 153.4 mg/l, respectively. The initial step in the biodegradation pathway of MG by P. pulmonicola YC32 was a reduction or N-demethylation reaction. We achieved a decolorization efficiency of 85.2% with 50mg/l MG in the immobilized P. pulmonicola YC32 continuous column system. This is the first report on the application of a continuous column system to decolorize MG using a microorganism.
Chen
Chih-Yu
CY
Department of Tourism, Hsing Wu College, Taipei 244, Taiwan, ROC.
Kuo
Jong-Tar
JT
Cheng
Chiu-Yu
CY
Huang
Yu-Tang
YT
Ho
I-Hsin
IH
Chung
Ying-Chien
YC
D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012394:Rosaniline Dyes / Q000378:metabolism*
D004396:Coloring Agents; D012394:Rosaniline Dyes; C005095:malachite green