title: NK cells from tuberculous pleurisy express high ICAM-1 levels and exert stimulatory effect on local T cells.
abstract: Tuberculous pleurisy, one of the most common manifestations of extrapulmonary tuberculosis, is characterized by a T-cell-mediated hypersensitivity reaction along with a Th1 immune profile. In this study, we investigated functional cross-talk among T and NK cells in human tuberculous pleurisy. We found that endogenously activated pleural fluid-derived NK cells express high ICAM-1 levels and induce T-cell activation ex vivo through ICAM-1. Besides, upon in vitro stimulation with monokines and PAMP, resting peripheral blood NK cells increased ICAM-1 expression leading to cellular activation and Th1 polarization of autologous T cells. Furthermore, these effects were abolished by anti-ICAM-1 Ab. Hence, NK cells may contribute to the adaptive immune response by a direct cell-contact-dependent mechanism in the context of Mycobacterium tuberculosis infection.
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Schierloh
authors.forename: Noemí
authors.initials: N
authors.lastname: Yokobori
authors.forename: Laura
authors.initials: L
authors.lastname: Geffner
authors.forename: Luciana
authors.initials: L
authors.lastname: Balboa
authors.forename: María M
authors.initials: MM
authors.lastname: Romero
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Musella
authors.forename: Mercedes
authors.initials: M
authors.lastname: Alemán
authors.forename: Jorge
authors.initials: J
authors.lastname: Castagnino
authors.forename: Juan
authors.initials: J
authors.lastname: Basile
authors.forename: Silvia S
authors.initials: SS
authors.lastname: de la Barrera
authors.forename: Eduardo
authors.initials: E
authors.lastname: Abbate
authors.forename: María C
authors.initials: MC
authors.lastname: Sasiain
mesh_terms: D000328:Adult; D040881:CD11a Antigen / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D002450:Cell Communication / Q000276:immunology; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D018664:Interleukin-12 / Q000494:pharmacology; D019409:Interleukin-15 / Q000494:pharmacology; D020382:Interleukin-18 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008074:Lipoproteins / Q000494:pharmacology; D008875:Middle Aged; D009169:Mycobacterium tuberculosis; D017856:Phosphotransferases (Phosphate Group Acceptor); D013601:T-Lymphocytes / Q000276:immunology*; D014396:Tuberculosis, Pleural / Q000276:immunology*
chemical_list: D040881:CD11a Antigen; D019002:CD56 Antigen; D019409:Interleukin-15; D020382:Interleukin-18; D008070:Lipopolysaccharides; D008074:Lipoproteins; D018799:Intercellular Adhesion Molecule-1; D018664:Interleukin-12; C069172:2,3-bis(palmitoyloxy)-2-propyl-1-palmitoylcysteine; D017856:Phosphotransferases (Phosphate Group Acceptor); C059169:polyphosphate AMP phosphotransferase; D003545:Cysteine