title: Suppressed-priming PCR, a novel concept of DNA quantification based on PCR kinetics.
abstract: The concept of an unbiased DNA quantification method is proposed based on PCR kinetics. A set of PCRs was performed with a dilution series of primers, which differently limited DNA amplification. The difference in amplification efficiency between the DNA templates reflected the estimates of their relative amounts. This concept might serve as a theoretical base for further development of accurate and robust PCR-based quantification methods.
authors.affiliation: National Institute for Agro-Environmental Sciences, 3-1-3 Kannon-dai, Tsukuba, Ibaraki 305-8604, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Takemoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okada
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004247:DNA