title: Establishment and characterisation of salmon louse (Lepeophtheirus salmonis (Krøyer 1837)) laboratory strains.
abstract: The salmon louse (Lepeophtheirus salmonis (Krøyer 1837)) is an ectoparasitic copepod which represents a major pathogen of wild and farmed salmonid fishes in the marine environment. In order to facilitate research on this ecologically and economically important parasite, a hatchery and culturing system permitting the closure of the life-cycle of L. salmonis in the laboratory was developed. Here, the hatchery system, breeding practices, and selected louse strains that have been maintained in culture in the period 2002-2009 are presented. The hatchery and culture protocol gave rise to predictable hatching of larvae and infections of host fish, permitting the cultivation of specific strains of L. salmonis for 22 generations. Both in- and out-bred lice and mutant colour strains have been established, and some of these strains were characterised by microsatellite DNA markers confirming their pedigree. No evidence of inbreeding depression, fitness or morphological changes was observed in any of the strains cultured. It is suggested that the culturing system, and the strains produced represent a significant resource for future research on this parasite.
authors.affiliation: Institute of Marine Research, Nordnes, Norway. larsh@imr.no
authors.forename: Lars A
authors.initials: LA
authors.lastname: Hamre
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Glover
authors.forename: Frank
authors.initials: F
authors.lastname: Nilsen
mesh_terms: D000818:Animals; D000830:Animals, Laboratory / Q000145:classification / Q000235:genetics / Q000254:growth & development; D033342:Copepoda / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D005393:Fish Diseases / Q000469:parasitology*; D005838:Genotype; D018895:Microsatellite Repeats; D009154:Mutation; D010274:Parasitology / Q000295:instrumentation / Q000379:methods; D012474:Salmon / Q000469:parasitology*