Age-dependent decrease in the interaction of beta-tubulin with estrogen receptor alpha transactivation domain in mouse brain.
In the previous paper, we reported that four proteins of 100 kDa, 80 kDa, 68 kDa and 50 kDa from the mouse brain interacted with estrogen receptor (ER) alpha-transactivation domain (TAD) and 68 kDa protein showed age and sex dependent changes. Here, we describe the identification of 50 kDa protein as beta-tubulin and changes in its interaction with age and sex in mouse brain. It is a microtubule-associated protein which binds to activation function (AF)-1 region of ERalpha-TAD and is involved in estrogen signaling. The extent of interaction of mouse ERalpha-TAD with beta-tubulin was higher in adult female as compared to old female and adult male. However, the expression of beta-tubulin showed no significant change with age and sex. Such age and sex dependent alteration in the interaction of beta-tubulin might account for the estrogen-mediated brain functions during aging.
Ghosh
Swati
S
Biochemistry and Molecular Biology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Thakur
M K
MK
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D008970:Molecular Weight; D017434:Protein Structure, Tertiary; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015533:Transcriptional Activation; D014404:Tubulin / Q000378:metabolism*
D047628:Estrogen Receptor alpha; D014404:Tubulin