title: Application of slicing of one-dimensional gels with subsequent slice-by-slice mass spectrometry for the proteomic profiling of human liver cytochromes P450.
abstract: Sequential thin slicing of one-dimensional electrophoresis gels followed by slice-by-slice mass spectrometry to allow protein identification was used to produce a proteomic map for cytochromes P450. Parallel MALDI-TOF-MS and LC-MS/MS analyses were performed. Combination of the two MS methods increased the quality of protein identification. We have proposed an efficient approach to obtain a comprehensive profile of drug-metabolizing enzymes in the liver that can be used to differentiate between polymorphic variants of cytochromes P450.
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Lisitsa
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Petushkova
authors.forename: Herbert
authors.initials: H
authors.lastname: Thiele
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Moshkovskii
authors.forename: Viktor G
authors.initials: VG
authors.lastname: Zgoda
authors.forename: Irina I
authors.initials: II
authors.lastname: Karuzina
authors.forename: Aleksey L
authors.initials: AL
authors.lastname: Chernobrovkin
authors.forename: Oleg G
authors.initials: OG
authors.lastname: Skipenko
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000595:Amino Acid Sequence; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D013058:Mass Spectrometry / Q000379:methods*; D008862:Microsomes, Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000235:genetics; D040901:Proteomics / Q000379:methods*
chemical_list: D010455:Peptides; D003577:Cytochrome P-450 Enzyme System