Comparative flavonoids contents of selected herbs and associations of their radical scavenging activity with antiproliferative actions in V79-4 cells.
To elucidate the health benefit of herbal teas on the cytotoxicity induced by H(2)O(2) in V79-4 cells, herbal extracts and its flavonoids were tested using lactate dehydrogenase release and determining intracellular reactive oxygen species generation and antioxidant activity with superoxide radical scavenging assay. Significant decrease in cell viability was observed on V79-4 cells treated with H(2)O(2) (1 mM), while herbal extracts and its flavonoids including catechin and epigallocatechin gallate prevented the LDH release from H(2)O(2) cytotoxicity. Total catechin contents of green tea (65.6 mg/g of dry matter) were significantly higher than other herbal teas (35.8 to 1.2 mg/g of DM). The relative concentration of the 4 major tea catechins ranked EGCG > EGC > EC > C. Green tea exhibited the lowest IC(50) values (2 g fresh herb/100 mL) of superoxide radical scavenging activity among the tested herbal tea, which indicates powerful antioxidant activity in O(2)(*-) radicals scavenging, followed by black tea, dandelion, hawthorn, rose hip, chamomile.
Yoo
Kyung Mi
KM
Dept. of Food and Nutrition, Research Inst. of Human Ecology, Seoul Natl. Univ., Seoul, South Korea.
Hwang
In-Kyeong
IK
Moon
BoKyung
B
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D019610:Cytoprotection; D005419:Flavonoids / Q000032:analysis* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000633:toxicity; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013045:Species Specificity; D013662:Tea / Q000737:chemistry* / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013662:Tea; D002392:Catechin; D006861:Hydrogen Peroxide