Bacterial community structure and dominant bacteria in activated sludge from a 70 degrees C ultrasound-enhanced anaerobic reactor for treating carbazole-containing wastewater.
Status of, and changes in, the bacterial communities at two acclimation stages (with- and without-ultrasound) in a small 70 degrees C ultrasound-enhanced anaerobic reactor for treating carbazole-containing wastewater reactor were analyzed by PCR-DGGE and real-time PCR techniques. PCR-DGGE results indicated that a large number of bands occurred in the whole sludge samples. Pseudomonas sp., Comamonas sp., and Diaphorobacter sp. were identified as being able to utilize carbazole as a carbon source, survive in an anaerobic and ultra-high-temperature environment and become dominant bacterial taxa during the with-ultrasound stage in the reactor. Total bacterial density in the with-ultrasonic stages was 10 x higher than in the without-ultrasonic treatment. The proportion of Pseudomonas was relatively stable at 0.13%-0.15% in both acclimation stages, which indicates that Pseudomonas can flourish and promote carbazole degradation either with or without-ultrasound. These studies provide information on carbazole degradation under ultra-high-temperature conditions in an anaerobic environment.
Tan
Yufei
Y
The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing 100871, China.
Ji
Guodong
G
D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D002227:Carbazoles / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D013010:Sonication / Q000379:methods*; D013696:Temperature; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D002227:Carbazoles; D012722:Sewage; D014874:Water Pollutants, Chemical; C041514:carbazole