title: Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect.
abstract: Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect, a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.
authors.affiliation: Febit biomed gmbh, D-69120 Heidelberg, Germany. nadine.schracke@febit.de
authors.forename: Nadine
authors.initials: N
authors.lastname: Schracke
authors.forename: Tobias
authors.initials: T
authors.lastname: Kornmeyer
authors.forename: Marcel
authors.initials: M
authors.lastname: Kränzle
authors.forename: Peer F
authors.initials: PF
authors.lastname: Stähler
authors.forename: Daniel
authors.initials: D
authors.lastname: Summerer
authors.forename: Markus
authors.initials: M
authors.lastname: Beier
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*