Effect of antifungal gels incorporated into a tissue conditioning material on the growth of Candida albicans.
OBJECTIVE
The aim of this study was to examine the effectiveness of antifungal gels incorporated into a tissue conditioner which inhibits the growth of Candida albicans in vitro.


BACKGROUND
The release of drugs from relining materials has been demonstrated earlier. However, the incorporation of antifungal agents in gel form has not yet been studied.


METHODS
Visco-gel(®) tissue conditioner was prepared with chlorhexidine digluconate and miconazole in gel form in a concentration of 5, 10, 15, 20 and 25% by volume. Sample discs were prepared and placed on Sabouraud Dextrose Agar (SDA) plates which had been previously inoculated with C. albicans, and incubated aerobically at 37 °C. To investigate antifungal activity over time, Visco-gel discs containing 20%v/v miconazole were prepared and immersed in water for different time periods before being placed on SDA plates inoculated with C. albicans.


RESULTS
Chlorhexidine digluconate gel added to tissue conditioner had no inhibition effect on the growth of C. albicans. Incorporation of miconazole gave a dose-related inhibitory effect on candidal growth. Immersion of the discs in water showed an inverse relationship between time of immersion and degree of inhibition.


CONCLUSIONS
Miconazole added in gel form to Visco-gel(®) had an inhibitory effect on the growth of C. albicans in vitro.
Radnai
Marta
M
Department of Prosthodontics, Faculty of Dentistry, University of Szeged, Szeged, Hungary. martaradnai@yahoo.com
Whiley
Rob
R
Friel
Tim
T
Wright
Paul S
PS
D000891:Anti-Infective Agents, Local / Q000008:administration & dosage; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000737:chemistry; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002626:Chemistry, Pharmaceutical; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives; D003822:Denture Liners; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D005782:Gels; D006801:Humans; D007101:Immersion; D005779:Immunodiffusion; D008422:Materials Testing; D008768:Methylmethacrylates / Q000008:administration & dosage; D008825:Miconazole / Q000008:administration & dosage; D009172:Mycology / Q000379:methods; D013997:Time Factors; D014016:Tissue Conditioning, Dental / Q000379:methods*; D014783:Viscosity; D014867:Water / Q000737:chemistry
D000891:Anti-Infective Agents, Local; D000935:Antifungal Agents; D001672:Biocompatible Materials; D005782:Gels; D008768:Methylmethacrylates; D014867:Water; D008825:Miconazole; C009931:poly(ethylmethacrylate); C010882:chlorhexidine gluconate; D002710:Chlorhexidine