Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors.
Viral vectors represent a tractable system that can efficiently introduce an exogenous gene into different target cells and are thus a potentially powerful genetic manipulation tool. In our current study, we investigated the infection efficiency of mammalian virus vectors, adenoviruses (Ads), adeno-associated viruses, and lentiviruses to the Sertoli cell line and the newly established cell line from a single embryo in zebrafish. Among the viral vectors tested, Ads showed the highest infection efficiency of 10(7)-10(8) green fluorescent protein-transducing units (gtu)/mL in zebrafish cells. In addition, the adenoviral vector was also infected at 10(5) gtu/mL in the medaka testicular somatic cell line that was established from the testes of p53-deficient mutant. Further, we found that Ads could successfully infect cultured male zebrafish germ cells. Our results thus indicate that the adenoviral vector could be used as a chromosomally nonintegrating vector system in zebrafish.
Kawasaki
Toshihiro
T
Genetic Strains Research Center, National Institute of Genetics, Yata, Mishima, Japan.
Saito
Kenji
K
Mitsui
Kaoru
K
Ikawa
Masahito
M
Yamashita
Masakane
M
Taniguchi
Yoshihito
Y
Takeda
Shunichi
S
Mitani
Kohnosuke
K
Sakai
Noriyoshi
N
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D017930:Genes, Reporter; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics; D008297:Male; D009990:Oryzias / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000378:metabolism / Q000821:virology; D014162:Transfection / Q000379:methods*; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism