Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes.
We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized.
Dang
Hieu M
HM
Omura
Gen
G
Umano
Toshiyuki
T
Yamagiwa
Masatomo
M
Kajiyama
Shin'ichiro
S
Ozeki
Yasuyuki
Y
Itoh
Kazuyoshi
K
Fukui
Kiichi
K
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000032:analysis*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014018:Tissue Distribution
D006454:Hemoglobins