title: Culturing ovarian somatic and germline stem cells of Drosophila.
abstract: This unit describes how to collect, culture, and establish stable cell lines of ovarian somatic and germline stem cells of Drosophila. We also describe a protocol for culturing embryonic cells that overexpress growth factors, which serve as a source for conditioned medium.
authors.affiliation: Ibaraki University, Ibaraki, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Niki
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004330:Drosophila / Q000166:cytology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D010053:Ovary / Q000166:cytology*; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D014020:Tissue Extracts; D016707:Tissue Fixation
chemical_list: D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D014020:Tissue Extracts