ADFP promoter polymorphism associated with marbling score in Korean cattle.
Marbling score (MS) is the major trait that affects carcass quality in beef cattle. In this study, we investigated the association between genetic polymorphisms of the adipose differentiation-related protein gene (ADFP) and carcass traits in Korean cattle (also known as Hanwoo). Using direct DNA sequencing in 24 unrelated Korean cattle, 25 novel polymorphisms were identified within all exons and their flanking regions of ADFP, including the promoter region (1.5 kb). Among them, 21 polymorphic sites were selected for genotyping in the beef cattle (n = 425). Statistical analyses revealed that one promoter polymorphism (c.-56-18A > G) was associated with MS (P = 0.009). The "A" allele of c.-56-18A > G exerted a lowering effect on MS, e.g., the lowest MS was found in "A/A" (MS = 2.09 +/- 1.23), intermediate in "A/G" (MS = 2.11 +/- 1.31), and the highest in "G/G" (MS = 2.47 +/- 1.47). Our findings suggest that these polymorphisms in ADFP might be important genetic factors involved in carcass quality in beef cattle.
Cheong
Hyun Sub
HS
Department of Life Science, Sogang University, Seoul 121-742, Korea.
Yoon
Du-Hak
DH
Bae
Joon Seol
JS
Kim
Lyoung Hyo
LH
Kim
Eun Mi
EM
Kim
Ji On
JO
Hong
Jin
J
Kim
Naesoo
N
Shin
Hyoung Doo
HD
D050154:Adiposity / Q000235:genetics*; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D007723:Korea; D015810:Linkage Disequilibrium / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*
D008565:Membrane Proteins