Spin labeling EPR.
Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling. Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the sensory rhodopsin-transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis and the photosynthetic reaction center from Rhodobacter sphaeroides R26.
Klare
Johann P
JP
Physics Department, University of Osnabrück, Barbarastr. 7, 49076, Osnabrück, Germany.
Steinhoff
Heinz-Jürgen
HJ
D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry; D008958:Models, Molecular; D011487:Protein Conformation; D013113:Spin Labels; D013997:Time Factors
D008565:Membrane Proteins; D013113:Spin Labels