Phellinins A1 and A2, new styrylpyrones from the culture broth of Phellinus sp. KACC93057P: I. Fermentation, taxonomy, isolation and biological properties.
Novel styrylpyrones, phellinins A1 and A2, were isolated together with known styrylpyrone compounds, hispidin and 1,1-distyrylpyrylethan, from the cultured broth of Phellinus sp. KACC93057P. These compounds were purified by solvent partition, Sephadex LH-20 column chromatography, C(18)-solid phase extraction and finally by reversed-phase (ODS) TLC. To identify the phellinin producer Phellinus sp. KACC93057P, the ribosomal DNA (rDNA) internal transcribed space regions containing 5.8 rDNA were sequenced and compared with those of the known Phellinus isolates. Phellinus sp. KACC93057P was 94.8% identical to P. baumii and P. linteus, all of which did not produce phellinins A1 and A2. These compounds significantly scavenged free radicals such as 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and superoxide.
Lee
In-Kyoung
IK
Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, Korea.
Seo
Geon-Sik
GS
Jeon
Nak Beom
NB
Kang
Hee-Wan
HW
Yun
Bong-Sik
BS
D001487:Basidiomycota / Q000145:classification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D011753:Pyrones / Q000302:isolation & purification* / Q000494:pharmacology; D013343:Styrenes / Q000302:isolation & purification* / Q000494:pharmacology
D016166:Free Radical Scavengers; D011753:Pyrones; D013343:Styrenes; C545889:phellinin A1; C545890:phellinin A2