Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection.
The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5%) the DON concentration was above the LOQ (380ng/g), in six samples (10.9%) DON was detected but could not be quantified (>LOD (200ng/g), <LOQ). In seven conventional samples (two pasta, two cookie, two snack and one feed sample) but only in one organic sample (a snack) the DON concentration was >LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.
Klinglmayr
Christine
C
Department of Analytical and Food Chemistry, University of Vienna, Austria.
Nöbauer
Katharina
K
Razzazi-Fazeli
Ebrahim
E
Cichna-Markl
Margit
M
D000821:Animal Feed / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D058870:Food, Organic / Q000032:analysis; D007163:Immunosorbent Techniques; D009183:Mycotoxins; D044367:Phase Transition; D013056:Spectrophotometry, Ultraviolet; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum
D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol