title: Polyethyleneimine coating enhances the cellular uptake of mesoporous silica nanoparticles and allows safe delivery of siRNA and DNA constructs.
abstract: Surface-functionalized mesoporous silica nanoparticles (MSNP) can be used as an efficient and safe carrier for bioactive molecules. In order to make the MSNP a more efficient delivery system, we modified the surface of the particles by a functional group that enhances cellular uptake and allows nucleic acid delivery in addition to traditional drug delivery. Noncovalent attachment of polyethyleneimine (PEI) polymers to the surface not only increases MSNP cellular uptake but also generates a cationic surface to which DNA and siRNA constructs could be attached. While efficient for intracellular delivery of these nucleic acids, the 25 kD PEI polymer unfortunately changes the safety profile of the MSNP that is otherwise very safe. By experimenting with several different polymer molecular weights, it was possible to retain high cellular uptake and transfection efficiency while reducing or even eliminating cationic MSNP cytotoxicity. The particles coated with the 10 kD PEI polymer were particularly efficient for transducing HEPA-1 cells with a siRNA construct that was capable of knocking down GFP expression. Similarly, transfection of a GFP plasmid induced effective expression of the fluorescent protein in >70% cells in the population. These outcomes were quantitatively assessed by confocal microscopy and flow cytometry. We also demonstrated that the enhanced cellular uptake of the nontoxic cationic MSNP enhances the delivery of the hydrophobic anticancer drug, paclitaxel, to pancreatic cancer cells. In summary, we demonstrate that, by a careful selection of PEI size, it is possible to construct cationic MSNP that are capable of nucleotide and enhanced drug delivery with minimal or no cytotoxicity. This novel use of a cationic MSNP extends its therapeutic use potential.
authors.affiliation: Division of NanoMedicine, Department of Medicine, University of California, Los Angeles, California 90095, USA.
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.forename: Michael
authors.initials: M
authors.lastname: Kovochich
authors.forename: Monty
authors.initials: M
authors.lastname: Liong
authors.forename: Huan
authors.initials: H
authors.lastname: Meng
authors.forename: Sanaz
authors.initials: S
authors.lastname: Kabehie
authors.forename: Saji
authors.initials: S
authors.lastname: George
authors.forename: Jeffrey I
authors.initials: JI
authors.lastname: Zink
authors.forename: Andre E
authors.initials: AE
authors.lastname: Nel
mesh_terms: D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D055598:Chemical Phenomena; D004247:DNA / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D006801:Humans; D051379:Mice; D050997:Nanomedicine; D053758:Nanoparticles / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry*; D016062:Porosity; D034741:RNA, Small Interfering / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity
chemical_list: D004337:Drug Carriers; D034741:RNA, Small Interfering; D012822:Silicon Dioxide; D011094:Polyethyleneimine; D004247:DNA; D017239:Paclitaxel