Preparation of highly sensitive protein array using reactive polymer.
We have discovered a novel protein immobilization method, i.e., a "Three-Dimensional Nanostructured Protein Hydrogel" (3-D NPH), which is composed of protein-reactive polymer hybrid nanoparticles to detect protein-protein interactions. The 3-D NPH can be easily prepared by spotting a protein/reactive polymer mixture on a substrate. The resulting 3-D NPH is characterized by large amounts of immobilized proteins and a novel porous structure.The 3-D NPH technology was applied to immobilize streptavidin (SA) onto Au-coated surface for surface plasmon resonance imaging (SPRi). By using 3-D NPH method, it was possible to improve the sensitivity of protein-protein interactions drastically comparing to the conventional protein immobilization method.
Shiroya
Toshifumi
T
Mitsubishi Chemical Group Science and Technology, Inc., Yokohama, Japan.
Tanaka
Hiroyuki
H
Hanasaki
Minako
M
Takeuchi
Hisao
H
D006046:Gold; D020100:Hydrogels; D055767:Immobilized Proteins; D008967:Molecular Biology / Q000379:methods; D053758:Nanoparticles; D011108:Polymers; D040081:Protein Array Analysis / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods; D019809:Streptavidin; D020349:Surface Plasmon Resonance / Q000379:methods
D020100:Hydrogels; D055767:Immobilized Proteins; D011108:Polymers; D006046:Gold; D019809:Streptavidin