title: Functional analysis of the Autographa californica nucleopolyhedrovirus IAP1 and IAP2.
abstract: The Autographa californica nucleopolyhedrovirus (AcMNPV) contains three apoptosis suppressor genes: p35, iap1 and iap2. AcMNPV P35 functions as a pancaspase inhibitor, but the function of IAP1 and IAP2 has not been entirely resolved. In this paper, we analyze the function of IAP1 and IAP2 in detail. AcMNPV with p35-deletion inhibited the apoptosis of BTI-Tn-5B1-4 (Tn-Hi5) cells induced by a Helicoverpa armigera single nucleocapsid NPV (HearNPV) infection and rescued the replication of HearNPV and BV production in these cells. Transient-expression experiments indicated that both IAP1 and IAP2 suppress apoptosis of Tn-Hi5 cells during HearNPV infection. Recombinant HearNPVs expressing AcMNPV iap1, iap2 and p35, respectively, not only prevented apoptosis but also allowed HearNPV to replicate in Tn-Hi5 cells. However, the iap1, iap2 and p35 genes when expressed in HearNPV were unable to rescue BV production. These results indicate that both AcMNPV iap1 and iap2 function independently as apoptosis inhibitors of and are potential host range factors.
authors.affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: XianDong
authors.initials: X
authors.lastname: Zeng
authors.forename: Fang
authors.initials: F
authors.lastname: Nan
authors.forename: ChangYong
authors.initials: C
authors.lastname: Liang
authors.forename: JianHua
authors.initials: J
authors.lastname: Song
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Just M
authors.initials: JM
authors.lastname: Vlak
authors.forename: XinWen
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D005784:Gene Amplification; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D017384:Sequence Deletion; D018411:Spodoptera / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000235:genetics; D014779:Virus Replication
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D014764:Viral Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus