title: Simultaneous determination of alprazolam and fluoxetine hydrochloride in tablet formulations by high-performance column liquid chromatography and high-performance thin-layer chromatography.
abstract: This paper describes validated HPLC and HPTLC methods for simultaneous determination of alprazolam (ALP) and fluoxetine hydrochloride (FXT) in pure powder and formulation. The HPLC separation was achieved on a Nucleosil C8 column (150 mm length, 4.6 mm id, 5 microm particle size) using acetonitrile-phosphate buffer pH 5.5 (45 + 55, v/v) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using acetone-toluene-ammonia (6.0 + 3.5 + 0.5, v/v/v) as the mobile phase. Quantification in the HPLC method was achieved with UV detection at 230 nm over the concentration range 4-14 microg/mL for both drugs, with mean recovery of 99.95 +/- 0.38 and 99.85 +/- 0.56% for ALP and FXT, respectively. Quantification in the HPTLC method was achieved with UV detection at 230 nm over the concentration range of 400-1400 ng/spot for both drugs, with mean recovery of 99.32 +/- 0.45 and 99.78 +/- 0.81% for ALP and FXT, respectively. These methods are simple, precise, and sensitive, and they are applicable for the simultaneous determination of ALP and FXT in pure powder and formulations.
authors.affiliation: Sardar Patel University, A.R. College of Pharmacy & G.H. Patel Institute of Pharmacy, Vallabh Vidyanagar 388120, Gujarat, India. rashmru@gmail.com
authors.forename: Rashmin B
authors.initials: RB
authors.lastname: Patel
authors.forename: Mrunali R
authors.initials: MR
authors.lastname: Patel
authors.forename: Madhira B
authors.initials: MB
authors.lastname: Shankar
authors.forename: Kashyap K
authors.initials: KK
authors.lastname: Bhatt
mesh_terms: D000525:Alprazolam / Q000032:analysis*; D014151:Anti-Anxiety Agents / Q000032:analysis*; D018687:Antidepressive Agents, Second-Generation / Q000032:analysis*; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005473:Fluoxetine / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013607:Tablets
chemical_list: D014151:Anti-Anxiety Agents; D018687:Antidepressive Agents, Second-Generation; D007202:Indicators and Reagents; D012996:Solutions; D013607:Tablets; D005473:Fluoxetine; D000525:Alprazolam