Evaluation of a nine-locus variable-number tandem-repeat scheme for typing of Pseudomonas aeruginosa.
Repeat numbers at nine variable-number tandem-repeat (VNTR) loci were determined for 177 isolates of Pseudomonas aeruginosa representing 77 strains distinguished by pulsed-field gel electrophoresis (PFGE). Eight loci provided for discrimination similar to that provided by PFGE, with variation at the ninth locus (ms61) sometimes allowing discrimination within a PFGE-defined type. The Liverpool and Midlands 1 strains, which are common among patients with cystic fibrosis in the UK, could be unambiguously identified by their characteristic VNTR profiles. In rare cases, the repeat number at the ninth locus alone provided discrimination among isolates that were distinct according to PFGE. In each case, the two isolates shared the same bla(OXA-50-like) allele and belonged to the same oprD sequence type group, supporting the VNTR results in suggesting that they are similar.
Turton
J F
JF
Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, London, UK. jane.turton@hpa.org.uk
Turton
S E
SE
Yearwood
L
L
Yarde
S
S
Kaufmann
M E
ME
Pitt
T L
TL
D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000150:complications; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D018272:Porins / Q000235:genetics; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D006113:United Kingdom; D001618:beta-Lactamases / Q000235:genetics
D004269:DNA, Bacterial; D018272:Porins; C077157:OprD protein, Pseudomonas aeruginosa; D001618:beta-Lactamases