title: Partial nitritation treatment of underground brine waste with high ammonium and salt content.
abstract: Underground brine waste containing high concentrations of ammonium and with a salinity of 3% is usually generated during the production of methane gas and iodine in the gas field of Chiba Prefecture, Japan. In this study, one swim-bed reactor, packed with a novel acrylic fiber biomass carrier (Biofringe), was applied to the partial nitritation treatment of this kind of underground brine waste. A stable nitrite production rate of 1.6 kg NO(2)-N m(-3) d(-1) was obtained under a nitrogen loading rate of 3.0 kg-N m(-3) d(-1), at a pH of 7.5 and a temperature of 25 degrees C. Nitrate production was negligible and the effluent NO(2)-N/NO(x)-N ratio was above 98% due to the successful inhibition of nitrite-oxidizing bacterial activity. Free ammonia was considered to be the main factor for inhibiting the activity of nitrite-oxidizing bacteria. A microbial community shift was demonstrated by 16S rRNA analysis, and it was shown that the ammonium-oxidizing bacteria became the predominant species after successful nitrite accumulation was observed.
authors.affiliation: Kumamoto University, Kurokami, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shinohara
authors.forename: Sen
authors.initials: S
authors.lastname: Qiao
authors.forename: Taichi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishiyama
authors.forename: Takao
authors.initials: T
authors.lastname: Fujii
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kaiho
authors.forename: Zafar
authors.initials: Z
authors.lastname: Bhatti
authors.forename: Kenji
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000032:analysis* / Q000737:chemistry; D012965:Sodium Chloride / Q000032:analysis; D014865:Waste Disposal, Fluid / Q000379:methods; D018508:Water Purification / Q000379:methods
chemical_list: D009566:Nitrates; D009573:Nitrites; D000644:Quaternary Ammonium Compounds; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts; C017082:brine; D012965:Sodium Chloride