Antiinflammatory and immunoregulatory effects of total glucosides of Yupingfeng powder.
BACKGROUND
Yupingfeng, a traditional Chinese complex prescription, has been used efficaciously in China for the cure and prevention of inflammatory diseases related to immunodeficiency such as allergic rhinitis and chronic bronchitis. However, the active components of this prescription remain unclear. The present study focused on investigating the antiinflammatory and immunoregulatory effects of the glucosidic extract from Yupingfeng.


METHODS
We tested animal models for ear swelling induced by dimethylbenzene in mice; palm swelling induced by carregeenin and granuloma induced by cotton pellet in rats; level of haemolysin, antibody generation by the splenic cells, delayed hypersensitivity and T cell subsets in spleen of immunosuppressed mice.


RESULTS
Glucosidic extract of 24 mg/kg, 48 mg/kg and 96 mg/kg significantly inhibited mice's ear swelling induced by dimethylbenzene. Similarly glucosidic extract of 16 mg/kg, 32 mg/kg and 64 mg/kg inhibited rats' palm swelling induced by carregeenin and granuloma induced by cotton pellet. Glucosidic extract of 24 mg/kg, 48 mg/kg and 96 mg/kg improved the IgM level in serum and level of haemolysin in splenocytes in mice immunosuppressed by cyclophosphamide. Delayed hypersensitivity in mice suppressed by cyclophosphamide was enhanced by glucosidic extract of 24 mg/kg, 48 mg/kg and 96 mg/kg. These results suggested that Yupingfeng could recover humoral and cellular immune function in mice with immunosuppression. Glucosidic extract of 48 mg/kg and 96 mg/kg significantly resisted the immunosuppressive mice ear swelling and maintained it at nearly normal level. The enhanced, delayed hypersensitivity actions of glucosidic extract, suppressed by cyclophosphamide, might be brought about by inducing TH cell and regulating T lymphocytes subset.


CONCLUSIONS
The glucosidic extract from Yupingfeng has antiinflammatory and immunoregulation action, suggesting that these glucosides are the principal active components of the traditional Chinese prescription Yupingfeng.
Gao
Jian
J
Department of Pharmacy of the First Affiliated Hospital, Anhui Medical University, Hefei, China.
Li
Jun
J
Shao
Xu
X
Jin
Yong
Y
Lü
Xiong-wen
XW
Ge
Jin-fang
JF
Huang
Yan
Y
Zhang
Lei
L
Chen
Lin
L
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002351:Carrageenan / Q000633:toxicity; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D005960:Glucosides / Q000627:therapeutic use*; D006099:Granuloma / Q000139:chemically induced / Q000188:drug therapy; D006168:Guinea Pigs; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D010031:Otitis / Q000139:chemically induced / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014992:Xylenes / Q000633:toxicity
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007166:Immunosuppressive Agents; D014992:Xylenes; C532967:yupingfeng; D002351:Carrageenan