title: Isoprenoid biosynthesis via the MEP pathway: in vivo Mössbauer spectroscopy identifies a [4Fe-4S]2+ center with unusual coordination sphere in the LytB protein.
abstract: The MEP pathway for the biosynthesis of isoprene units is present in most pathogenic bacteria, in the parasite responsible for malaria, and in plant plastids. This pathway is absent in animals and is accordingly a target for the development of antimicrobial drugs. LytB, also called IspH, the last enzyme of this pathway catalyzes the conversion of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) using an oxygen sensitive iron sulfur cluster. The exact nature of this iron sulfur cluster is still a matter of debate. We have used (57)Fe Mössbauer spectroscopy to investigate the LytB cluster in whole E. coli cells and in the anaerobically purified enzyme: In LytB an unusual [4Fe-4S](2+) cluster is attached to the protein by three conserved cysteines and contains a hexacoordinated iron linked to three sulfurs of the cluster and three additional oxygen or nitrogen ligands.
authors.affiliation: Université de Strasbourg, Institut de Chimie, UMR CNRS UDS 7177, 4 rue Blaise Pascal, 67070 Strasbourg, France. mseemann@chimie.u-strasbg.fr
authors.forename: Myriam
authors.initials: M
authors.lastname: Seemann
authors.forename: Karnjapan
authors.initials: K
authors.lastname: Janthawornpong
authors.forename: Julia
authors.initials: J
authors.lastname: Schweizer
authors.forename: Lars H
authors.initials: LH
authors.lastname: Böttger
authors.forename: Adam
authors.initials: A
authors.lastname: Janoschka
authors.forename: Anne
authors.initials: A
authors.lastname: Ahrens-Botzong
authors.forename: Erasmienne Ngouamegne
authors.initials: EN
authors.lastname: Tambou
authors.forename: Olaf
authors.initials: O
authors.lastname: Rotthaus
authors.forename: Alfred X
authors.initials: AX
authors.lastname: Trautwein
authors.forename: Michel
authors.initials: M
authors.lastname: Rohmer
mesh_terms: D000693:Anaerobiosis; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D007501:Iron; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D015204:Spectroscopy, Mossbauer; D013403:Sugar Phosphates / Q000378:metabolism*; D013455:Sulfur; D013729:Terpenes / Q000378:metabolism*
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D029968:Escherichia coli Proteins; D013403:Sugar Phosphates; D013729:Terpenes; D013455:Sulfur; D007501:Iron; D010088:Oxidoreductases; C109866:ispH protein, E coli; D004896:Erythritol