[Plasma membrane-related Ca(2+)-ATPase-1 gene silencing promotes insulin secretion in islet beta cells NIT].
OBJECTIVE
To assess the effect of RNA interference-mediated gene silencing of plasma membrane-related Ca(2+)-ATPase-1 (PMR1) gene on the insulin secretion in islet beta cells NIT-1 in vitro.


METHODS
A small interfering RNA duplex (siPMR1) corresponding to the nucleotides 337-357 of mouse PMR1 cDNA was introduced into NIT-1 cells via liposomes. The gene silencing effect was assessed by RT-PCR, and the total insulin level in the transfected cells was measured by radioimmunoassay.


RESULTS
Transfection with siPMR1 resulted in obviously reduced PMR1 expression and increased insulin secretion in NIT-1 cells.


CONCLUSIONS
The synthesized siPMR1 can significantly silence the expression of PMR1 and promote the secretion of insulin in the islet cells in vitro, which shed light on further studies of RNAi-based therapy of diabetes.
Fang
Yan-ping
YP
Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Ji
Ai-min
AM
Yang
Yue-lian
YL
Feng
Qiao-fei
QF
Sun
Liang
L
Liu
Hong
H
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000172:deficiency* / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D051379:Mice; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D007328:Insulin; D012333:RNA, Messenger; C492024:Atp2c1 protein, mouse; D000252:Calcium-Transporting ATPases