The evaluation of putative endogenous control housekeeping genes for real-time polymerase chain reaction expression studies in Moraxella catarrhalis.
Comparisons of endogenous control genes in real-time polymerase chain reaction gene expression studies involving Moraxella catarrhalis are rare. This study shows that a combination of the iron sequestering gene copB and 16S rRNA genes would be useful for lineage 1 (16S rRNA type 1) isolates, but not lineage 2 (16S rRNA types 2 and 3) isolates.
Hays
John P
JP
Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands. j.hays@erasmusmc.nl
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D005798:Genes, Bacterial; D001936:Moraxella catarrhalis / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; C093197:CopB protein, Moraxella; D012336:RNA, Ribosomal, 16S