Generation of pluripotent stem cells from patients with type 1 diabetes.
Type 1 diabetes (T1D) is the result of an autoimmune destruction of pancreatic beta cells. The cellular and molecular defects that cause the disease remain unknown. Pluripotent cells generated from patients with T1D would be useful for disease modeling. We show here that induced pluripotent stem (iPS) cells can be generated from patients with T1D by reprogramming their adult fibroblasts with three transcription factors (OCT4, SOX2, KLF4). T1D-specific iPS cells, termed DiPS cells, have the hallmarks of pluripotency and can be differentiated into insulin-producing cells. These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy.
Maehr
René
R
Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
Chen
Shuibing
S
Snitow
Melinda
M
Ludwig
Thomas
T
Yagasaki
Lisa
L
Goland
Robin
R
Leibel
Rudolph L
RL
Melton
Douglas A
DA
D000328:Adult; D053687:Adult Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002478:Cells, Cultured; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D004707:Endoderm / Q000166:cytology / Q000378:metabolism; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D006801:Humans; D000090062:Kruppel-Like Factor 4; D008297:Male; D008954:Models, Biological; D010179:Pancreas / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D014157:Transcription Factors / Q000378:metabolism
C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors