title: Unintended changes in protein expression revealed by proteomic analysis of seeds from transgenic pea expressing a bean alpha-amylase inhibitor gene.
abstract: Seeds of genetically modified (GM) peas (Pisum sativum L.) expressing the gene for alpha-amylase inhibitor-1 (alphaAI1) from the common bean (Phaseolus vulgaris L. cv. Tendergreen) exhibit resistance to the pea weevil (Bruchus pisorum). A proteomic analysis was carried out to compare seeds from GM pea lines expressing the bean alphaAI1 protein and the corresponding alphaAI1-free segregating lines and non-GM parental line to identify unintended alterations to the proteome of GM peas due to the introduction of the gene for alphaAI1. Proteomic analysis showed that in addition to the presence of alphaAI1, 33 other proteins were differentially accumulated in the alphaAI1-expressing GM lines compared with their non-GM parental line and these were grouped into five expression classes. Among these 33 proteins, only three were found to be associated with the expression of alphaAI1 in the GM pea lines. The accumulation of the remaining 30 proteins appears to be associated with Agrobacterium-mediated transformation events. Sixteen proteins were identified after MALDI-TOF-TOF analysis. About 56% of the identified proteins with altered accumulation in the GM pea were storage proteins including legumin, vicilin or convicilin, phaseolin, cupin and valosin-containing protein. Two proteins were uniquely expressed in the alphaAI1-expressing GM lines and one new protein was present in both the alphaAI1-expressing GM lines and their alphaAI1-free segregating lines, suggesting that both transgenesis and transformation events led to demonstrable changes in the proteomes of the GM lines tested.
authors.affiliation: Australian Research Council Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biology, The Australian National University, Canberra ACT, Australia. Hancai.chen@anu.edu.au
authors.forename: Hancai
authors.initials: H
authors.lastname: Chen
authors.forename: Greg
authors.initials: G
authors.lastname: Bodulovic
authors.forename: Prudence J
authors.initials: PJ
authors.lastname: Hall
authors.forename: Andy
authors.initials: A
authors.lastname: Moore
authors.forename: Thomas J V
authors.initials: TJ
authors.lastname: Higgins
authors.forename: Michael A
authors.initials: MA
authors.lastname: Djordjevic
authors.forename: Barry G
authors.initials: BG
authors.lastname: Rolfe
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D051379:Mice; D018532:Pisum sativum / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D037121:Plant Lectins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D055314:Seed Storage Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism
chemical_list: D010455:Peptides; D037121:Plant Lectins; D010940:Plant Proteins; D020543:Proteome; D055314:Seed Storage Proteins; C061510:alpha-amylase inhibitor, Phaseolus vulgaris