title: [Influence of F protein of hepatitis C virus subtype 1b inhibits on human hepatocellular carcinoma HepG2 cell apoptosis].
abstract: OBJECTIVE
  To investigate the effects of F protein of hepatitis C virus subtype 1b on the apoptosis of human hepatocellular carcinoma HepG2 cells.
  
  
  METHODS
  HepG2 cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP and pcDNA3.0-F-EGFP-HepG2 strain was exposed to Act-D and tumor necrosis factor alpha (TNF alpha) treatment in order to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-HepG2, negative control pcDNA3.0-C-EGFP-HepG2 and blank control HepG2. Annexin V-FITC/PI of flow cytometry was performed to determine the number of apoptotic cells. DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotic cells.
  
  
  RESULTS
  pcDNA3.0-F-EGFP- HepG2 cell strain showed a much delayed apoptosis as well as obviously lowering the apoptotic rate when compared with the pcDNA3.0-HepG2 strain and HepG2 strain (P < 0.001).
  
  
  CONCLUSIONS
  The introduction and expression of extraneous gene (the F gene of hepatitis C virus subtype 1b) could significantly inhibit the apoptosis of HepG2 cells.
authors.affiliation: School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-zhao
authors.initials: XZ
authors.lastname: Deng
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Zhong-can
authors.initials: ZC
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Le
authors.initials: L
authors.lastname: Feng
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Ding
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005822:Genetic Vectors; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014758:Viral Core Proteins / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D014758:Viral Core Proteins; C433031:hepatitis C protein F, Hepatitis C virus