title: [Microenvironment for the hematopoietic differentiation of human embryonic stem cells].
abstract: OBJECTIVE
  To investigate the roles of human fetal liver stromal cells (hFLSCs) and human fetal bone marrow stromal cells (hFBMSCs) in the hematopoietic differentiation of human embryonic stem cells and analyze their gene expression profile changes.
  
  
  METHODS
  The embryonic bodies on day 4 were cocultured with hFLSCs or hFBMSC in the presence of cytokines. Flow cytometry was performed after 8 days of induction to detect the expressions of the hemangioblast markers KDR and CD34, and the differential gene expression profiles between hFBMSC and hFLSCs were examined by cDNA microarray analysis.
  
  
  RESULTS
  Eight days after the induction, (1.06-/+0.20)% of the hFLSCs and (8.8-/+1.49)% of the hFBMSCs were positive for KDR, with the positivity rates for CD34 of (1.25-/+0.16)% and (9.17-/+2.10)%, respectively. In hFLSCs and hFBMSCs cultures, 0.9-/+0.36 and 10.6-/+0.63 hemagioblast-like cell colonies were found, respectively. cDNA microarray analysis showed that 240 genes were highly expressed in hFBMSCs, and 21 genes related to secreted cytokines, cell adhesion molecules and extracellular matrix proteins were highly expressed.
  
  
  CONCLUSIONS
  The microenvironment including the cell matrix protein and cytokines secreted by the hFBMSCs might play an important role in hemangioblastic differentiation of human bone marrow stromal cells in vitro.
authors.affiliation: Institute of Reproduction and Stem Cell Engineering, Central South University, State Human Stem Cell Engineering Research Center, Changsha, China. wjlx2000@yahoo.com.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Ge
authors.initials: G
authors.lastname: Ling
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Zhao
authors.forename: Guang-Xiu
authors.initials: GX
authors.lastname: Lu
mesh_terms: D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology*; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology*; D005333:Fetus; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D017154:Stromal Cells / Q000502:physiology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D040301:Vascular Endothelial Growth Factor Receptor-2