title: [Silencing of COX-2 in nasopharyngeal carcinoma cells with a shRNAmir lentivirus vector].
abstract: OBJECTIVE
  To construct a miR-155-based lentivirus vector to induce cyclooxygenase-2 gene silencing in nasopharyngeal carcinoma (NPC) cells by expressing anti-COX-2 shRNAmir.
  
  
  METHODS
  miR-155-based anti-COX-2 shRNAmir template was synthesized and inserted into pLVTHM plasmid. The recombinant pLVTHM/shRNAmir was transfected into 293FT cells for packaging the lentivirus vector. After infection with the lentivirus vector, the GFP-positive cells were screened by flow cytometry, and COX-2 mRNA level was detected by RT-PCR.
  
  
  RESULTS
  Restriction digestion and DNA sequencing confirmed successful construction of the anti-COX-2 vector pLVTHM/shRNAmir. A subline of C666-1 cells was established after infection with the lentivirus vector, and the COX-2 expression in the cells was stably silenced.
  
  
  CONCLUSIONS
  The shRNAmir lentivirus vector constructed may serve as an effective COX-2 inhibitor, which may facilitate future studies of gene therapy of NPC.
authors.affiliation: Department of Otolaryngology, Nanfang Hospital, Southern Medical University, Guangzhou, China. climber1999y@yahoo.com.cn
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Lu
authors.forename: Xiong
authors.initials: X
authors.lastname: Liu
authors.forename: Shun-Jin
authors.initials: SJ
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human