title: Immunoassay for cadmium detection and quantification.
abstract: OBJECTIVE
  To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES).
  
  
  METHODS
  An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated.
  
  
  RESULTS
  IC-ELISA showed an IC50 of 45.6 microg/L with a detection limit of 1.95 microg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998.
  
  
  CONCLUSIONS
  IC-ELISA can detect and quantify cadmium residue in environmental or food samples.
authors.affiliation: School of Biosciences & Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
authors.forename: Gong-Liang
authors.initials: GL
authors.lastname: Liu
authors.forename: Ju-Fang
authors.initials: JF
authors.lastname: Wang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Liang
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002104:Cadmium / Q000737:chemistry*; D004785:Environmental Pollutants / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D004785:Environmental Pollutants; D002104:Cadmium