title: Spin labeling EPR.
abstract: Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling. Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the sensory rhodopsin-transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis and the photosynthetic reaction center from Rhodobacter sphaeroides R26.
authors.affiliation: Physics Department, University of Osnabrück, Barbarastr. 7, 49076, Osnabrück, Germany.
authors.forename: Johann P
authors.initials: JP
authors.lastname: Klare
authors.forename: Heinz-Jürgen
authors.initials: HJ
authors.lastname: Steinhoff
mesh_terms: D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry; D008958:Models, Molecular; D011487:Protein Conformation; D013113:Spin Labels; D013997:Time Factors
chemical_list: D008565:Membrane Proteins; D013113:Spin Labels