Boron containing two-photon absorbing chromophores. 2. Fine tuning of the one- and two-photon photophysical properties of pyrazabole based fluorescent bioprobes.
New boron containing two-photon absorbing fluorophores have been prepared. Centered on a pyrazabole central core, various conjugated systems and end groups were investigated to modulate their physicochemical properties (alkoxy, diphenylamino, and boron dipyromethene groups). One and two-photon photophysical characterizations were performed, showing efficient fluorescence in organic solvents. High two-photon absorption cross sections were determined in the 500-800 nm range. Two-photon excited microscopy images were also obtained with these new boron containing fluorescent bioprobes with laser intensities in the milliwatt range.
Hayek
Ali
A
Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg (UMR CNRS 7213), Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch, France.
Bolze
Frédéric
F
Bourgogne
Cyril
C
Baldeck
Patrice L
PL
Didier
Pascal
P
Arntz
Youri
Y
Mély
Yves
Y
Nicoud
Jean-François
JF
D000042:Absorption; D015374:Biosensing Techniques / Q000379:methods*; D001895:Boron / Q000737:chemistry*; D003116:Color; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D001895:Boron