title: Screening for reactive metabolites using electro-optical arrays featuring human liver cytosol and microsomal enzyme sources and DNA.
abstract: We demonstrate for the first time the combination of human liver cytosol and microsomal enzyme sources into an electro-optical array to screen for reactive metabolites produced in multi-enzyme metabolic processes.
authors.affiliation: Department of Chemistry, University of Connecticut, 55 N. Eagleville Rd, Storrs, CT, USA.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhao
authors.forename: John B
authors.initials: JB
authors.lastname: Schenkman
authors.forename: James F
authors.initials: JF
authors.lastname: Rusling
mesh_terms: D000123:Acetyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D001711:Biotransformation; D003600:Cytosol / Q000201:enzymology*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018736:DNA Adducts / Q000032:analysis / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods; D015946:Ethylene Dibromide / Q000378:metabolism; D005449:Fluorenes / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D008163:Luminescent Measurements / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D008862:Microsomes, Liver / Q000201:enzymology*; D053758:Nanoparticles / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D018736:DNA Adducts; D005449:Fluorenes; D009942:Organometallic Compounds; D004364:Pharmaceutical Preparations; C492100:bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))ruthenium(II); D015946:Ethylene Dibromide; C012177:2-aminofluorene; D004247:DNA; D000123:Acetyltransferases; D005982:Glutathione Transferase; D005978:Glutathione