Specific enrichment methods for glycoproteome research.
Glycosylation is, by far, one of the most common and important post-translational modifications and becomes a target for proteomic research. A key challenge in glycoproteome research is the development of fast and effective enrichment strategies for high-throughput glycosylation analysis. Different kinds of glycan-capturing anchors have been developed and successfully applied to glyco-specific enrichment in large-scale glycosylation identification in the past few years. In this paper, we highlight several examples on various types of enrichment methods that have been utilized to specifically capture glycopeptides/glycoproteins for subsequent mass spectrometric analysis.
Zhang
Lijuang
L
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China.
Lu
Haojie
H
Yang
Pengyuan
P
D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000639:trends; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D020543:Proteome / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*
D029742:Caenorhabditis elegans Proteins; D006023:Glycoproteins; D020543:Proteome