title: Toxicity and carcinogenicity of ozone in combination with 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and dibutyl phthalate in B6C3F1 mice for 16 and 32 weeks.
abstract: OBJECTIVE
  To evaluate the toxic and carcinogenic potential of ozone alone or in combination with 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibutyl phthalate (DBP).
  
  
  METHODS
  Male and female B6C3F1 mice were exposed, through inhalation, intravenous administration and diet, to 0.5 ppm of ozone, 1.0 mg/kg of NNK and 5000 ppm of DBP, individually and in combination for 16 and 32 weeks.
  
  
  RESULTS
  No treatment-related death was seen, but significant differences in body and organ weights between control and treated mice were observed during the study. No incidence of lung tumor incidence was recorded in mice exposed to either ozone alone or combined treatment. Oviductal carcinomas were observed in female mice exposed to ozone or DBP alone for 16 weeks and ozone in combination with NNK and DBP for 32 weeks.
  
  
  CONCLUSIONS
  Although ozone alone and in conjunction with NNK and/or DBP does not induce lung cancer under our experimental conditions, they induce oviductal carcinomas in B6C3F1 mice.
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. jeffmkim@mit.edu
authors.forename: Min Young
authors.initials: MY
authors.lastname: Kim
authors.forename: M Yung
authors.initials: MY
authors.lastname: Cho
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000473:pathology; D000818:Animals; D002274:Carcinogens, Environmental / Q000008:administration & dosage / Q000633:toxicity*; D003993:Dibutyl Phthalate / Q000008:administration & dosage / Q000633:toxicity*; D004334:Drug Administration Schedule; D004359:Drug Therapy, Combination; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D009602:Nitrosamines / Q000008:administration & dosage / Q000633:toxicity*; D009929:Organ Size; D010053:Ovary / Q000187:drug effects / Q000473:pathology; D010126:Ozone / Q000008:administration & dosage / Q000633:toxicity*; D012727:Sex Characteristics; D013737:Testis / Q000187:drug effects / Q000473:pathology
chemical_list: D002274:Carcinogens, Environmental; D009602:Nitrosamines; D003993:Dibutyl Phthalate; D010126:Ozone; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone