Quantitative screening of stilbenes and zeranol and its related residues and natural precursors in veal liver by gas chromatography-mass spectrometry.
An existing gas chromatography-mass spectrometry-based quantitative screening method for the regulatory analysis of the resorcylic acid lactones zeranol, taleranol, and zearalanone and the stilbene anabolic steroids diethylstilbestrol and dienestrol was extended to include natural precursors of zeranol (zearalenone, alpha-zearalenol, and beta-zearalenol) in veal liver. No changes in sample preparation were required; the instrumental conditions were selected to effect a suitable chromatographic separation and detection of the analytes. Validation experiments were performed to verify the performance and applicability of the extended method for the quantitative screening of the original and additional analytes in veal liver in the concentration range from 0.5 to 2.0 microg/kg. The limits of detection were 0.08-0.19 microg/kg. The limits of quantitation were 0.27-0.64 microg/kg. Recoveries were 29-67%. Combined relative measurement uncertainty estimates were 6-21%.
Dickson
Leslie C
LC
Centre for Veterinary Drug Residues, Canadian Food Inspection Agency, 116 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 2R3. les.dickson@inspection.gc.ca
Costain
Roderick
R
McKenzie
Del
D
Fesser
Adrian C E
AC
Macneil
James D
JD
D000818:Animals; D004350:Drug Residues / Q000032:analysis*; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D012756:Sheep; D013267:Stilbenes / Q000032:analysis* / Q000378:metabolism; D015025:Zearalenone / Q000032:analysis* / Q000378:metabolism; D015029:Zeranol / Q000032:analysis* / Q000378:metabolism
D004968:Estrogens, Non-Steroidal; D013267:Stilbenes; D015025:Zearalenone; D015029:Zeranol