[Detection of BDNF expression after injection of BDNF gene lentiviral vector in rat hippocampus].
OBJECTIVE
To explore the method of injecting the BDNF gene lentiviral vector in rat hippocampus and detecting its expression.


METHODS
SD rats were randomly divided into four groups: sham operated group (SH), normal saline group (NS), GFP gene lentiviral vector injection group (GFP) and BDNF lentiviral vector injection group (BDNF).The expression of BDNF was detected by Western blot, RT-PCR and situ hybridization (ISH) at day 7, 14 and 30 after the injection.


RESULTS
BDNF Gene lentiviral vector can up-regulated the expression of BDNF in rat hippocampus. The result of RT- PCR and Western blot detection show that the level of mRNA and the protein level was remarkably increased. The result of in-situ hybridization showed the stable expression and distribution of BDNF.


CONCLUSIONS
Successfully established the method of injecting BDNF gene lentiviral vector in rat hippocampus and detected the high level of BDNF expression following with the time, which lays the basis for its application in the treatment of the neurodamaged disease.
Zhang
Jing-yu
JY
Department of Neuology, the Fourth Hospital of Harbin Medical University, Harbin 150001, China.
Li
Jin-xing
JX
Sun
Yong-qi
YQ
Zhao
Hong
H
Yang
Zi-chao
ZC
D000818:Animals; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D005822:Genetic Vectors; D006624:Hippocampus / Q000378:metabolism*; D017403:In Situ Hybridization; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D019208:Brain-Derived Neurotrophic Factor