Lactobacillus and Bifidobacterium diversity in horse feces, revealed by PCR-DGGE.
Lactobacillus equi, Lactobacillus hayakitensis, Lactobacillus johnsonii, and Weissella confusa/cibaria were the dominant species in 12 South African horses. The Bifidobacterium-group was detected in the feces of only one of the 12 horses. Sequencing of the nested-PCR amplicon identified the Bifidobacterium-group as Parascardovia denticolens. Cell numbers of L. equi, L. hayakitensis, and W. confusa/cibaria were consistent in all samples. P. denticolens, Bifidodobacterium pseudolongum, and a phylogenetic relative of Alloscardovia omnicolens were rarely detected. L. equigenerosi, a dominant species in Japanese horses, was detected in the fecal samples of only one horse.
Endo
Akihito
A
Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa. pegaman@hotmail.co.jp
Futagawa-Endo
Y
Y
Dicks
L M T
LM
D000818:Animals; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D005243:Feces / Q000382:microbiology*; D005260:Female; D006736:Horses / Q000235:genetics / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004269:DNA, Bacterial