Profile and relative concentrations of fatty acids in corn and soybean seeds from transgenic and isogenic crops.
In this work 44 fatty acids, which were analyzed as methyl esters by GC/MS in scan mode, have been determined in genetically modified corn and soybean seeds. Their relative concentrations have been compared with those of isogenic lines grown in the same conditions. Studied compounds comprised saturated and unsaturated fatty acids, including cis/trans isomers and minor fatty acids. A classical soxhlet extraction and an accelerated solvent extraction have been assayed to extract the fatty compounds from seeds and the GC separation has been carried out on a biscyanopropylpolysiloxane chromatographic column. Soxhlet extraction was selected as the most convenient and applied to compare the samples. Specific compounds, which could denote the origin of the crop have not been observed, but for some sample pairs, significant differences have been found in relation to the percentage of certain acids; the highest differences for major acids were 4.1% in corn and 4.8% in soybean. The concentrations of long chain acids such as 24:0, 26:0 and 28:0 were higher in some isogenic lines whereas the concentrations of short chain acids such as 6:0, 8:0, 9:0, 10:0 and 12:0 were higher in their transgenic counterparts.
Jiménez
J J
JJ
I.U.CINQUIMA, Analytical Chemistry Group, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain. jjimenez@qa.uva.es
Bernal
J L
JL
Nozal
M J
MJ
Toribio
L
L
Bernal
J
J
D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007536:Isomerism; D030821:Plants, Genetically Modified / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
D005227:Fatty Acids