Determination of airborne particle-associated benz[a]anthracene-7,12-quinone using high-performance liquid chromatography with in-line reduction and fluorescence detection.
A simple and sensitive method for measuring airborne particle-associated benz[a]anthracene-7,12-quinone (BaAQ) based on two-dimensional high-performance liquid chromatography (HPLC) with fluorescence detection was established. The system involves an ODS column for sample clean up, a 6-port switching valve, an ODS column for trapping the fraction containing BaAQ, an ODS column for sample separation, and a Pt-Rh catalytic column for reduction of BaAQ to a corresponding fluorescent compound. The accuracy of the assay, as applied to airborne particulate sample extracts spiked with known amounts of BaAQ was 94-108%. The detection limit was 97 fmol per injection (signal-to-noise ratio=3), and the calibration range was from 1 to 100 pmol with excellent proportionality (R(2)=0.9995). BaAQ in airborne particles collected in Osaka, Japan every 3 h was successfully measured using the established analytical method with simple sample preparation steps: ultrasonic extraction in organic solvent and concentration under reduced pressure and/or nitrogen stream.
Kameda
Takayuki
T
Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan. kameda@p.kanazawa-u.ac.jp
Goto
Tomoko
T
Toriba
Akira
A
Tang
Ning
N
Hayakawa
Kazuichi
K
D000393:Air Pollutants / Q000032:analysis*; D001551:Benz(a)Anthracenes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D052638:Particulate Matter / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D000393:Air Pollutants; D001551:Benz(a)Anthracenes; D052638:Particulate Matter; C077646:7,12-benz(a)anthraquinone