Neurofibromin homologs Ira1 and Ira2 affect glycerophosphoinositol production and transport in Saccharomyces cerevisiae.
Saccharomyces cerevisiae produces extracellular glycerophosphoinositol through phospholipase-mediated turnover of phosphatidylinositol and transports glycerophosphoinositol into the cell upon nutrient limitation. A screening identified the RAS GTPase-activating proteins Ira1 and Ira2 as required for utilization of glycerophosphoinositol as the sole phosphate source, but the RAS/cyclic AMP pathway does not appear to be involved in the growth phenotype. Ira1 and Ira2 affect both the production and transport of glycerophosphoinositol.
Bishop
Andrew C
AC
Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA.
Surlow
Beth A
BA
Anand
Puneet
P
Hofer
Katherine
K
Henkel
Matthew
M
Patton-Vogt
Jana
J
D001692:Biological Transport; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D020690:GTPase-Activating Proteins; C065725:IRA1 protein, S cerevisiae; C064960:IRA2 protein, S cerevisiae; D007295:Inositol Phosphates; D029701:Saccharomyces cerevisiae Proteins; C014575:glycerylphosphoinositol