Black-tea polyphenols suppress postprandial hypertriacylglycerolemia by suppressing lymphatic transport of dietary fat in rats.
Administration of black-tea polyphenols (BTP) at 100 and 200 mg/kg of body weight in rats suppressed postprandial hypertriacylglycerolemia in a dose-dependent manner. Administration of BTP also suppressed lymphatic recovery of (14)C-trioleoylglycerol in rats that were cannulated in the thoracic duct. BTP dose-dependently inhibited the activity of pancreatic lipase in vitro with an IC50 of 0.254 mg/mL. When purified theaflavins, which are components of BTP, were used, theaflavins with galloyl moieties, but not those without galloyl moiety, inhibited the activity of pancreatic lipase. Theaflavin-3,3'-digallate (TFDG) was more effective in inhibiting the activity of pancreatic lipase than epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and a mixture of EGCG and ECG. BTP and TFDG had a similar effect in inhibiting the activity of pancreatic lipase when the total polyphenol amount was adjusted to the same. BTP had no effect on micellar solubility of hydrolysis products of triacylglycerol. These results suggest that BTP suppressed postprandial hypertriacylglycerolemia by reducing triacylglycerol absorption via the inhibition of pancreatic lipase activity.
Kobayashi
Makoto
M
Central Research Institute, ITO EN, Ltd., Shizuoka 421-0516, Japan. m-kobayasi@itoen.co.jp
Ichitani
Masaki
M
Suzuki
Yuko
Y
Unno
Tomonori
T
Sugawara
Takashi
T
Yamahira
Takashi
T
Kato
Masaki
M
Takihara
Takanobu
T
Sagesaka
Yuko
Y
Kakuda
Takami
T
Ikeda
Ikuo
I
D000818:Animals; D001692:Biological Transport; D028241:Camellia sinensis / Q000737:chemistry; D004041:Dietary Fats / Q000378:metabolism*; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D015228:Hypertriglyceridemia / Q000188:drug therapy / Q000378:metabolism*; D008196:Lymph / Q000378:metabolism*; D008297:Male; D010636:Phenols / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D059808:Polyphenols; D019518:Postprandial Period; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry* / Q000378:metabolism
D004041:Dietary Fats; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea