title: Effect of salivary contamination on microleakage of resin composites placed with a self-etch adhesive in primary teeth: an in vitro study.
abstract: OBJECTIVE
  The purpose of this study was to investigate in vitro the effect of salivary contamination on the microleakage of resin composite restorations placed with a self-etching adhesive in primary teeth.
  
  
  METHODS
  Fifty-six extracted primary molars received standardized Class V preparations. The teeth were assigned to 7 groups (n=8): 1-layer and 2-layer application of the self-etching primer (Clearfil SE Bond) with no contamination (groups 1 and 2, respectively); saliva, SE primer, and drying (group 3); SE primer, drying, saliva, and drying (group 4); SE primer, drying, saliva, and rinsing/drying (group 5); SE primer, drying, saliva, drying, SE primer, and drying (group 6); and SE primer, drying, saliva, rinsing/drying, SE primer, and drying (group 7). For all specimens, the bonding agent (SE adhesive) and a composite were applied. After thermocycling and immersing in basic fuchsin, the teeth were sectioned and evaluated under a stereomicroscope. Data were analyzed using Kruskal-Wallis and Mann-Whitney tests.
  
  
  RESULTS
  The mean occlusal and gingival microleakage for groups 4 and 5 were significantly higher than that of groups 1 and 2 (P<.04). No statistically significant difference in microleakage among groups 1, 2, 6, and 7 was found (P=.09). The degree of microleakage in the gingival margins was significantly higher than that of occlusal margins (P=.027).
  
  
  CONCLUSIONS
  The self-etching adhesive was not sensitive to saliva when contamination occurred before primer application.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Marjan
authors.initials: M
authors.lastname: Fakhri
authors.forename: Bahman
authors.initials: B
authors.lastname: Seraj
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shahrabi
authors.forename: Pouria
authors.initials: P
authors.lastname: Motahhary
authors.forename: Tabassom
authors.initials: T
authors.lastname: Hooshmand
mesh_terms: D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding / Q000379:methods; D003737:Dental Cavity Preparation / Q000145:classification; D003763:Dental Leakage / Q000145:classification*; D003764:Dental Materials / Q000737:chemistry*; D003793:Dental Restoration, Permanent / Q000379:methods*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D003890:Desiccation; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008963:Molar / Q000648:ultrastructure; D019279:Resin Cements / Q000737:chemistry; D012394:Rosaniline Dyes; D012463:Saliva / Q000502:physiology*; D013499:Surface Properties; D013696:Temperature; D014094:Tooth, Deciduous / Q000648:ultrastructure*; D014867:Water / Q000737:chemistry
chemical_list: C112828:Clearfil AP-X; C438313:Clearfil SE Bond; D004396:Coloring Agents; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D019279:Resin Cements; D012394:Rosaniline Dyes; D014867:Water; C025485:basic fuchsin