title: Rapid detection of a point mutation in the parC gene associated with decreased susceptibility to fluoroquinolones in Mycoplasma bovis.
abstract: Comparison of the quinolone resistance-determining regions (QRDRs) in 42 Mycoplasma bovis clinical isolates revealed amino acid substitutions at both GyrA (position 83) and ParC (position 84) in 10/11 enrofloxacin-resistant strains. The mutation present in the parC QRDR was discriminative for enrofloxacin resistance by parC PCR-restriction fragment length polymorphism. Comparison of molecular profiles by insertion sequence typing suggests that the currently prevalent enrofloxacin-resistant M. bovis strain evolved by selection under field conditions from one of the susceptible strains.
authors.affiliation: Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, POB 12, Bet Dagan 50250, Israel. innal@moag.gov.il
authors.forename: I
authors.initials: I
authors.lastname: Lysnyansky
authors.forename: I
authors.initials: I
authors.lastname: Mikula
authors.forename: I
authors.initials: I
authors.lastname: Gerchman
authors.forename: S
authors.initials: S
authors.lastname: Levisohn
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D045682:Mycoplasma bovis / Q000187:drug effects* / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D027101:DNA Topoisomerase IV