title: Cytotoxicity of 1-aryl-3-buthylamino-1-propanone hydrochlorides against Jurkat and L6 cells.
abstract: 1-Aryl-3-buthylamino-1-propanone hydrochloride type mono Mannich bases were synthesized and their cytotoxicity was tested against transformed human T-lymphocytes (Jurkat cells) and rat skeletal muscle derived myoblasts (L6 cells). Aryl part was changed as phenyl in 1, 4-methylphenyl in 2, 4-chlorophenyl in 3, 4-fluorophenyl in 4, 4-bromophenyl in 5, 4-hydroxyphenyl in 6, 2-acethylthiophene in 7. Of the compounds synthesized, 2, 5, 6, and 7 are reported for the first time. Compounds 1-7 had 3.16, 3.13, 3.35, 2.87, 4.17, 2.60, and 3.04 times higher cytotoxic potency than the reference compound 5-fluorouracil (CAS 51-21-8) against Jurkat cells, respectively. Compounds 1, 3, 4, 5, 6, and 7 had 1.22, 1.46, 1.59, 2.18, 1.24, and 1.45 times higher cytotoxic potency than the reference compound 5-fluorouracil against L6 cells, respectively. Among the compounds tested, only compound 5 had almost equal cytotoxic potency with the reference compound melphalan (CAS 148-82-3) against Jurkat and L6 cells. All compounds synthesized showed higher cytotoxic activity against Jurkat cells compared with L6 cells. Specifically, compounds 1-7 had 2.05, 2.68, 1.82, 1.43, 1.51, 1.66, and 1.66 times higher cytotoxicity against Jurkat cells compared with L6 cells. In Jurkat cells, there was a significant negative correlation between Log P and IC50 values (correlation coefficient: -0.955, p = 0.03), which actually means a positive correlation between the Log P and the cytotoxic activity of the compounds. These results suggest that the most potent compound 5 (a 4-bromo derivative) against both cell lines may serve as a model compound to develop new cytotoxic agents for further studies.
authors.affiliation: Department of Physiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Gul
authors.forename: Ebru
authors.initials: E
authors.lastname: Mete
authors.forename: Mustafa
authors.initials: M
authors.lastname: Atalay
authors.forename: Mustafa
authors.initials: M
authors.lastname: Arik
authors.forename: Halise Inci
authors.initials: HI
authors.lastname: Gul
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D007202:Indicators and Reagents; D019169:Jurkat Cells; D009682:Magnetic Resonance Spectroscopy; D008352:Mannich Bases; D018485:Muscle Fibers, Skeletal / Q000187:drug effects; D032446:Myoblasts / Q000187:drug effects; D051381:Rats
chemical_list: D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D008352:Mannich Bases; D000096:Acetone