[Chronic persistent infections of anterior segment of eye: clinico-laboratory aspects].
OBJECTIVE
Improvement of therapy of chronic ophthalmic infectious diseases during assessment of functioning of different arms of immune system.


METHODS
Three hundred and fifty patients with chronic red-eye syndrome were tested by immunofluorescence assay on the presence of antigens of herpesviruses, adenoviruses and Chlamydia in samples from conjunctiva. Expression of 11 cytokines' genes was measured in peripheral blood mononuclear cells by reverse transcription polymerase chain reaction. Production of IFN-alpha and IFN-gamma, levels of serum and spontaneously produced interferon as well as level of susceptibility to the range of immunomodulating preparations were measured during study of interferon status in whole blood cells. Study of parameters of cytokine, interferon and immune statuses was performed in 70 patients. Counts of T- and B-lymphocytes, T-helpers, NK-cells as well as level of circulating immune complexes were measured during study of immune status.


RESULTS
Antigens of herpes simplex virus and adenoviruses were detected in samples from conjunctiva in 27% (95 persons) and 36% (126 persons) of patients respectively. Enhanced level of expression of several cytokines (IL-2, IL-4) in studied patients compared with healthy volunteers was observed. Expression levels of IL-12 and TNF-alpha mRNAs were, in opposite, in 2 - 3 times lower. Disorder of IFN-alpha and IFN-gamma synthesis on post-transciption level was observed in 60 - 90% of patients. Decrease of absolute numbers of total T-lymphocytes and T-helpers as well as increase of absolute number of NK-cells was noted in 20%, 25%, and 27% of patients respectively.


CONCLUSIONS
Assignment of individually oriented antiviral, antibacterial and immunomodulating therapy allowed to mitigate intensity of clinical symptoms in 30 -60% of patients with chronic persistent infections of anterior segment of eye.
Chernakova
G M
GM
Pirtskhalava
M A
MA
Guliaeva
O A
OA
Abaeva
M R
MR
Shapoval
I M
IM
Shcherbenko
V E
VE
Zuev
V A
VA
Mezentseva
M V
MV
D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000188:drug therapy* / Q000276:immunology; D000890:Anti-Infective Agents / Q000627:therapeutic use; D000956:Antigens, Viral / Q000032:analysis; D002689:Chlamydia / Q000187:drug effects; D002908:Chronic Disease; D003234:Conjunctivitis, Bacterial / Q000175:diagnosis / Q000188:drug therapy* / Q000276:immunology; D003236:Conjunctivitis, Viral / Q000175:diagnosis / Q000188:drug therapy* / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D006561:Herpes Simplex / Q000175:diagnosis / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use; D018655:Lymphocyte Count; D014141:Trachoma / Q000175:diagnosis / Q000188:drug therapy*
D000890:Anti-Infective Agents; D000956:Antigens, Viral; D016207:Cytokines; D007155:Immunologic Factors