title: In vitro examination of suspicious oral lesions using optical coherence tomography.
abstract: We compared findings of optical coherence tomography (OCT) with histopathological results of suspicious oral lesions to assess the feasibility of using OCT to identify malignant tissue. Thirty-four oral lesions from 27 patients had swept-source frequency-domain OCT. Four variables were assessed (changes in keratin, epithelial, and sub-epithelial layers, and identification of the basement membrane) and from this we calculated whether or not there were architectural changes. These data were then compared with histopathological results. Two clinicians, who were unaware of the clinical and histopathological diagnoses, decided whether biopsy was necessary. The basement membrane was recognised in only 15 oral lesions. OCT could identify diseased areas but could not provide a diagnosis or differentiate between lesions. The two clinicians, who recommended biopsy agreed in all cases. This pilot study confirms the feasibility of using OCT to identify architectural changes in malignant tissues.
authors.affiliation: Head & Neck Centre, University College London Hospital, London, United Kingdom. waseem wk1@yahoo.co.uk
authors.forename: Waseem
authors.initials: W
authors.lastname: Jerjes
authors.forename: Tahwinder
authors.initials: T
authors.lastname: Upile
authors.forename: Brendan
authors.initials: B
authors.lastname: Conn
authors.forename: Zaid
authors.initials: Z
authors.lastname: Hamdoon
authors.forename: Christian S
authors.initials: CS
authors.lastname: Betz
authors.forename: Gordon
authors.initials: G
authors.lastname: McKenzie
authors.forename: Hani
authors.initials: H
authors.lastname: Radhi
authors.forename: Michael
authors.initials: M
authors.lastname: Vourvachis
authors.forename: Mohammed
authors.initials: M
authors.lastname: El Maaytah
authors.forename: Ann
authors.initials: A
authors.lastname: Sandison
authors.forename: Amrita
authors.initials: A
authors.lastname: Jay
authors.forename: Colin
authors.initials: C
authors.lastname: Hopper
mesh_terms: D001485:Basement Membrane / Q000473:pathology; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000473:pathology; D003937:Diagnosis, Differential; D004848:Epithelium / Q000473:pathology; D004919:Erythroplasia / Q000175:diagnosis / Q000473:pathology; D005240:Feasibility Studies; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007633:Keratins / Q000032:analysis; D007972:Leukoplakia, Oral / Q000175:diagnosis / Q000473:pathology; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000473:pathology; D019226:Oral Ulcer / Q000175:diagnosis / Q000473:pathology; D010865:Pilot Projects; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000473:pathology; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*; D014062:Tongue Neoplasms / Q000175:diagnosis / Q000473:pathology
chemical_list: D007633:Keratins