title: Correlation between neutral alpha-glucosidase activity and sperm DNA fragmentation.
abstract: To evaluate the association between neutral alpha-glucosidase (NAG) activity and sperm DNA fragmentation (DFI), ejaculates from 24 men undergoing evaluation for sperm DNA damage as a part of infertility assessment were analysed. The mean +/- SD and range for the semen quality of the 24 ejaculates are as follows: volume (3.1 +/- 1.3, 1.8-6.0 ml); sperm concentration (45.6 +/- 41.1, 1.3-151.2 x 10(6) ml(-1)); sperm motility (52.8 +/- 28.8, 1-95%); sperm with fragmented DNA (17.6 +/- 15.4, 1.7-56.0%); sperm with immature chromatin (9.6 +/- 3.8, 2.5-19.1%); NAG activity (37.9 +/- 18.3, 4.4-75.3 mU ml(-1)). The only sperm parameter significantly correlated with neutral alpha-glucosidase is the percentage of sperm DFI [correlation coefficient (r) = 0.4376, P = 0.03].
authors.affiliation: Andrology Laboratory Services, Chicago, IL, USA.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: R
authors.initials: R
authors.lastname: Roussev
authors.forename: P
authors.initials: P
authors.lastname: Ahlering
authors.forename: R
authors.initials: R
authors.lastname: Sauer
authors.forename: C
authors.initials: C
authors.lastname: Coulam
authors.forename: R S
authors.initials: RS
authors.lastname: Jeyendran
mesh_terms: D000328:Adult; D053938:DNA Fragmentation; D006801:Humans; D007248:Infertility, Male; D008297:Male; D012044:Regression Analysis; D055101:Semen Analysis; D013053:Spectrophotometry; D013094:Spermatozoa / Q000201:enzymology* / Q000473:pathology*; D018709:Statistics, Nonparametric; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D000520:alpha-Glucosidases