title: The circulation of human astrovirus genotypes in the Central West Region of Brazil.
abstract: Out of 1,588 faecal samples of children taken from three locations of the Central West Region of Brazil, 57 were positive for astroviruses (HAstVs) using reverse transcription-polymerase chain reaction (RT-PCR). They were genotyped by nested RT-PCR and/or genomic sequencing. HAstV-1 (42.8%), HAstV-2 (23.2%), HAstV-3 (3.6%), HAstV-4 (14.3%) and HAstVs -5, -6, -7 and -8 (1.8% each) were detected. In Goiânia and Campo Grande, HAstV-1 was the most frequently detected genotype while in Brasília (DF) it was HAstV-2. Shifts in the circulation of astrovirus genotypes were observed in DF and Campo Grande. All samples collected by rectal swabs were viral negative. The astrovirus genotypes were detected in all age groups and there was no correlation between genotype and age group.
authors.affiliation: Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Setor Universitário, 74605-050 Goiânia, Brasil.
authors.forename: Paula Andreia
authors.initials: PA
authors.lastname: Silva
authors.forename: Rodrigo Alessandro Tôgo
authors.initials: RA
authors.lastname: Santos
authors.forename: Paulo Sérgio Sucasas
authors.initials: PS
authors.lastname: Costa
authors.forename: José Marcus Sócrates
authors.initials: JM
authors.lastname: Teixeira
authors.forename: Loreny Gimenes
authors.initials: LG
authors.lastname: Giugliano
authors.forename: Márcia Sueli Assis
authors.initials: MS
authors.lastname: Andreasi
authors.forename: José Paulo Gagliardi
authors.initials: JP
authors.lastname: Leite
authors.forename: Ecakart
authors.initials: E
authors.lastname: Schreier
authors.forename: Divina das Dôres de Paula
authors.initials: Dd
authors.lastname: Cardoso
mesh_terms: D019350:Astroviridae Infections / Q000175:diagnosis / Q000821:virology*; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology*; D005838:Genotype; D006801:Humans; D001257:Mamastrovirus / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction