title: Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells.
abstract: Human induced pluripotent stem (iPS) cells are similar to embryonic stem (ES) cells, and can proliferate intensively and differentiate into a variety of cell types. However, the hepatic differentiation of human iPS cells has not yet been reported. In this report, human iPS cells were induced to differentiate into hepatic cells by a stepwise protocol. The expression of liver cell markers and liver-related functions of the human iPS cell-derived cells were monitored and compared with that of differentiated human ES cells and primary human hepatocytes. Approximately 60% of the differentiated human iPS cells at day 7 expressed hepatic markers alpha fetoprotein and Alb. The differentiated cells at day 21 exhibited liver cell functions including albumin Asecretion, glycogen synthesis, urea production and inducible cytochrome P450 activity. The expression of hepatic markers and liver-related functions of the iPS cell-derived hepatic cells were comparable to that of the human ES cell-derived hepatic cells. These results show that human iPS cells, which are similar to human ES cells, can be efficiently induced to differentiate into hepatocyte-like cells.
authors.affiliation: The MOE Key Laboratory of Cell Proliferation and Differentiation, Department of cell biology, College of Life Sciences, Box 38, Peking University, Beijing 100871, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Liu
authors.forename: Dongxin
authors.initials: D
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Yong
authors.forename: Shuguang
authors.initials: S
authors.lastname: Duo
authors.forename: Xijun
authors.initials: X
authors.lastname: Song
authors.forename: Yushan
authors.initials: Y
authors.lastname: Guo
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Han
authors.initials: H
authors.lastname: Qin
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Yin
authors.forename: Chen
authors.initials: C
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Che
authors.forename: Shichun
authors.initials: S
authors.lastname: Lu
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Ding
authors.forename: Hongkui
authors.initials: H
authors.lastname: Deng
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D008112:Liver Glycogen / Q000096:biosynthesis; D012709:Serum Albumin / Q000378:metabolism; D000075462:Serum Albumin, Human; D014508:Urea / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000096:biosynthesis
chemical_list: C422455:ALB protein, human; D006023:Glycoproteins; D008112:Liver Glycogen; D012709:Serum Albumin; D000509:alpha-Fetoproteins; D014508:Urea; D003577:Cytochrome P-450 Enzyme System; D000075462:Serum Albumin, Human