Dopamine release at individual presynaptic terminals visualized with FFNs.
The nervous system transmits signals between neurons via neurotransmitter release during synaptic vesicle fusion. To observe neurotransmitter uptake and release from individual presynaptic terminals directly, we designed fluorescent false neurotransmitters as substrates for the synaptic vesicle monoamine transporter. Using these probes to image dopamine release in the striatum, we made several observations pertinent to synaptic plasticity. We found that the fraction of synaptic vesicles releasing neurotransmitter per stimulus was dependent on the stimulus frequency. A kinetically distinct "reserve" synaptic vesicle population was not observed under these experimental conditions. A frequency-dependent heterogeneity of presynaptic terminals was revealed that was dependent in part on D2 dopamine receptors, indicating a mechanism for frequency-dependent coding of presynaptic selection.
Zhang
Hui
H
Departments of Neurology, Columbia University.
Gubernator
Niko G
NG
Yue
Minerva
M
Staal
Roland G W
RG
Mosharov
Eugene V
EV
Pereira
Daniela
D
Balsanek
Vojtech
V
Vadola
Paul A
PA
Mukherjee
Bipasha
B
Edwards
Robert H
RH
Sulzer
David
D
Sames
Dalibor
D
D000818:Animals; D001551:Benz(a)Anthracenes / Q000032:analysis*; D003342:Corpus Striatum / Q000737:chemistry / Q000378:metabolism; D004298:Dopamine / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis*; D008297:Male; D051379:Mice; D009473:Neuronal Plasticity; D017729:Presynaptic Terminals / Q000737:chemistry* / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D009435:Synaptic Transmission
C541442:8-(2-amino-ethyl)-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-aza-benzo(de)anthracen-10-one; D001551:Benz(a)Anthracenes; D005456:Fluorescent Dyes; D017448:Receptors, Dopamine D2; D004298:Dopamine