title: Determination of serum ferritin using immunoturbidimetry or chemiluminescent detection in comparison with radioimmunoassay a compendium of a methodological juxtaposition.
abstract: BACKGROUND
  The implementation of a ferritin assay other that by the RIA technique may be necessary in several laboratories. Indeed the RIA method presents limitations for routine determination: use of radioactive isotopes, high cost of reagents and low capacity (manual technique). This method was not suitable for all laboratories.
  
  
  METHODS
  The aim of this study was to compare the IRMA method with non-isotope methods (immunoturbidimetric assay and chemiluminescence detection) for determination of ferritin in serum samples.
  
  
  RESULTS
  Analytical performances of Olympus-, Horiba ABX- and Beckman Coulter- ferritin are good. In a whole population (n = 124) linear regression analysis indicated excellent agreement between ferritin values obtained on all patients leading to a coefficient of determination r2 > 0.96, 0.95 and 0.96 with Olympus-Ferritin, Horiba ABX-ferritin, and Beckman-ferrritin, respectively. Results of Bland-Altman analysis demonstrated the excellent concordance of ferritin methods with samples.
  
  
  CONCLUSIONS
  Immunoturbidimetric methods, particularly the Horiba ABX method on Pentra 400 and immunoenzymometric assay with chemiluminescence detection with Beckman Coulter on Access 2, are a reasonable alternative to the established RIA for all laboratories.
authors.affiliation: Department of Biochemistry, Lapeyronie Hospital, Montpellier, France.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Dupuy
authors.forename: Laurent
authors.initials: L
authors.lastname: Debarge
authors.forename: Marine
authors.initials: M
authors.lastname: Poulain
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Badiou
authors.forename: Michel
authors.initials: M
authors.lastname: Rossi
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Cristol
mesh_terms: D005293:Ferritins / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007202:Indicators and Reagents; D008163:Luminescent Measurements / Q000379:methods; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012044:Regression Analysis; D012680:Sensitivity and Specificity
chemical_list: D007202:Indicators and Reagents; D005293:Ferritins