Development of a downscale sedimentation field flow fractionation device for biological event monitoring.
Classically described as a macroscale size-density based method, Sedimentation field flow fractionation (SdFFF) has been successfully used for cell sorting. The goal of this study was to develop a new SdFFF device for downscale applications, in particular for oncology research to rapidly monitor chemical biological event induction in a cell line. The development of a downscale SdFFF device required reduction of the separation channel volume. Taking advantage of a newly laboratory designed apparatus, channel volume was successfully decreased by reducing both length and breadth. To validate the apparatus and method, we used the well-known model of diosgenin dose-dependent induction of apoptosis or megakaryocytic differentiation in HEL cells. After a minute scale acquisition of a reference profile, the downscale device was able to perform fast, early, significant and reproducible monitoring of apoptosis and differentiation, two important biological mechanisms in the field of cancer research.
Bégaud-Grimaud
G
G
Laboratoire de Chimie Analytique et Bromatologie, EA 3842, Homéostasie Cellulaire et Pathologies, Université de Limoges, Faculté de Pharmacie, 2 rue du Docteur Marcland, 87025 Limoges Cedex, France.
Battu
S
S
Liagre
B
B
Beneytout
J L
JL
Jauberteau
M O
MO
Cardot
P J P
PJ
D017209:Apoptosis; D002454:Cell Differentiation; D002460:Cell Line; D004867:Equipment Design; D031521:Fractionation, Field Flow / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D008533:Megakaryocytes / Q000737:chemistry / Q000166:cytology