title: Detection of bluetongue serotype 4 in mouflons (Ovis aries musimon) from Spain.
abstract: Bluetongue serotype 4 (BTV4) has been detected for the first time in tissue samples from 2 mouflons (Ovis aries musimon) from the South of Spain, in a retrospective study. The samples included in this study had been fixed and paraffin-embedded for over a year prior to their analysis using a BTV group-specific and a BTV4-specific RT-PCR test. Lung and lymphatic nodes were found positive in both specimens. The amplified DNA was confirmed to be BTV4 by sequencing the RT-PCR products and comparing them with other sequences from GenBank. The combination of RNA extraction from paraffin-embedded samples and serotype-specific real-time RT-PCR assays provides the tools for the detection of BTV from samples stored for a long time. The results shown in this study set out the basis for a greater survey with fixed samples from different species of wild ruminants that the veterinary services have been collecting for years.
authors.affiliation: Animal Health Department, Veterinary Faculty, Universidad Complutense de Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain. belenrodriguez@sanidadanimal.info
authors.forename: Belén
authors.initials: B
authors.lastname: Rodríguez-Sánchez
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Sánchez-Cordón
authors.forename: Verónica
authors.initials: V
authors.lastname: Molina
authors.forename: María A
authors.initials: MA
authors.lastname: Risalde
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Pérez de Diego
authors.forename: José C
authors.initials: JC
authors.lastname: Gómez-Villamandos
authors.forename: José M
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
mesh_terms: D000818:Animals; D001483:Base Sequence; D001819:Bluetongue / Q000473:pathology / Q000821:virology*; D008168:Lung / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D034561:Sheep, Domestic / Q000821:virology*; D013030:Spain