title: Bacterial colonisation of collagen-coated polypropylene vaginal  mesh: are additional intraoperative sterility procedures useful?
abstract: OBJECTIVE
  The use of vaginally implanted polypropylene meshes in the treatment of prolapse is becoming increasingly popular. We set out to detect how often bacterial colonisation of the mesh occurs and if the intraoperative sterility procedures that are applied matter.
  
  
  METHODS
  In 64 consecutive women, bacterial colonisation was compared between two intraoperative sterility procedures. Culture swabs of the core mesh were taken during surgery, and the mesh arms removed at the end of surgery were cultured separately.
  
  
  RESULTS
  Sixty-seven implants were cultured. In 56 (83.6%) implants, a positive culture with vaginal bacteria was found with very low bacterial density (<10(3 )colony-forming units). No significant differences in bacterial species, density, clinical infection and erosion (two anterior and one posterior) were found between the two intraoperative sterility methods.
  
  
  CONCLUSIONS
  Colonisation of vaginally implanted mesh occurs frequently but in low bacterial densities, irrespective of the intraoperative sterility procedure used.
authors.affiliation: Department of Obstetrics and Gynaecology, Spaarne Hospital, 2130 AT Hoofddorp, The Netherlands. a.vollebregt@umcutrecht.nl
authors.forename: Astrid
authors.initials: A
authors.lastname: Vollebregt
authors.forename: Annet
authors.initials: A
authors.lastname: Troelstra
authors.forename: C Huub
authors.initials: CH
authors.lastname: van der Vaart
mesh_terms: D000368:Aged; D019072:Antibiotic Prophylaxis; D001424:Bacterial Infections / Q000517:prevention & control; D003094:Collagen; D005260:Female; D006801:Humans; D007430:Intraoperative Care / Q000379:methods*; D008875:Middle Aged; D056887:Pelvic Organ Prolapse / Q000601:surgery*; D011126:Polypropylenes; D013242:Sterilization / Q000379:methods*; D053825:Suburethral Slings / Q000382:microbiology*; D013526:Surgical Mesh / Q000382:microbiology*
chemical_list: D011126:Polypropylenes; D003094:Collagen