title: Lactobacillus strains stabilize intestinal microbiota in Japanese cedar pollinosis patients.
abstract: A randomized double-blind, placebo-controlled trial was conducted to ascertain the intestinal microbiota-altering properties of LGG and L. gasseri TMC0356 (TMC0356) in Japanese cedar Cryptomeria japonica pollinosis patients. Fecal bacteria communities were examined before and after fermented milk administration using culture, FISH and T-RFLPmethods. Test group subjects showed the presence of LGG and TMC0356 along with a significant increase in fecal lactobacilli (P < 0.001) after giving LGG and TMC0356 fermented milk. Culture and FISH analysis revealed no significant changes in other intestinal bacterial groups. Each subject exhibited a characteristic T-RFLP profile pattern that varied quantitatively and qualitatively with JCP shedding. Profile changes were observed in 53% of placebo group subjects and in 21% of test group subject's post-administration, indicating that LGG and TMC0356 suppressed intestinal microbiota changes in JCPsis patients. The results suggest that intestinal microbiota might be more sensitive to exposure to environmental allergens than expected from the results of general culture method studies. Stabilization of intestinal microbiota by selected probiotic strains such as LGG and TMC0356 could be beneficial to homeostasis of the intestinal microbiota and useful in the management of JCPsis.
authors.affiliation: Technical Research Laboratory, Takanashi Milk Products Co., Ltd, Yokohama, Kanagawa 241-0023, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kubota
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawase
authors.forename: Gaku
authors.initials: G
authors.lastname: Harata
authors.forename: Masaru
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: Seppo
authors.initials: S
authors.lastname: Salminen
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Iino
mesh_terms: D000328:Adult; D015373:Bacterial Typing Techniques / Q000379:methods; D016000:Cluster Analysis; D032485:Cryptomeria; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D004311:Double-Blind Method; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007564:Japan; D007778:Lactobacillus / Q000254:growth & development*; D008297:Male; D008875:Middle Aged; D010919:Placebos / Q000008:administration & dosage; D012150:Polymorphism, Restriction Fragment Length; D019936:Probiotics / Q000008:administration & dosage*; D006255:Rhinitis, Allergic, Seasonal; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D010919:Placebos