Evaluation of ID-PaGIA syphilis antibody test.
BACKGROUND
Laboratory diagnosis of syphilis is usually accomplished by serology. There are currently a large number of different commercial treponemal tests available that vary in format, sensitivity and specificity.


OBJECTIVE
To evaluate the ID-PaGIA Syphilis Antibody Test as an alternative to other specific treponemal tests for primary screening or confirmation of diagnosis.


METHODS
Serum samples from healthy adults (n = 100) were used for detection of specificity of ID-PaGIA. To evaluate sensitivity of ID-PaGIA serum samples (n = 101) from patients with confirmed or suspected syphilis were tested for syphilis antibodies with FTA-Abs IgM, ID-PaGIA, ELISA IgM and TPHA tests.


RESULTS
No false-positive results were found with ID-PaGIA. Sensitivity of various treponemal tests was the following: FTA-Abs IgM: 95.5%, ID-PaGIA and ELISA IgM: 94%, and TPHA 75%. The positive and negative predictive values of ID-PaGIA were 100 and 89.5%, respectively.


CONCLUSIONS
Compared with other treponemal tests ID-PaGIA has excellent sensitivity and specificity.
Naaber
Paul
P
Department of Medical Microbiology, Stavanger University Hospital, and United Laboratories, Tartu University Hospital, Estonia, Norway. paul.naaber@kliinikum.ee
Makoid
Ene
E
Aus
Anneli
A
Loivukene
Krista
K
Poder
Airi
A
D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards / Q000639:trends; D013587:Syphilis / Q000097:blood* / Q000175:diagnosis* / Q000382:microbiology; D013588:Syphilis Serodiagnosis / Q000379:methods / Q000592:standards* / Q000639:trends
D000907:Antibodies, Bacterial