title: Discrimination of healthy and neoplastic human colon tissues by ex vivo HR-MAS NMR spectroscopy and chemometric analyses.
abstract: The metabolic profile of human healthy and neoplastic colorectal tissues was obtained using ex vivo High-Resolution Magic Angle Spinning (HR-MAS) NMR spectroscopy. Principal Components Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) were applied to NMR data in order to highlight the biochemical differences between healthy and neoplastic colorectal tissues. The synergic combination of ex vivo HR-MAS NMR spectroscopy with Multivariate Data Analysis enables discrimination between healthy and tumoral colorectal tissues and identification of the increase of taurine, acetate, lactate, and lipids, and the decrease of polyols and sugars as tumoral characteristics. Moreover, it was found that macroscopically/histologically normal colorectal tissues, collected at least 15 cm from the adenocarcinoma, are characterized by a metabolic pattern quite similar to that typical of tumoral lesions. It was shown that ex vivo HR-MAS NMR spectroscopy, performed on intact specimens, may be of great potentiality in the clinical evaluation of human neoplastic colorectal tissues and that the biochemical data represent the molecular basis for an accurate and noninvasive clinical applications of in vivo NMR spectroscopy.
authors.affiliation: Dipartimento di Biochimica "G. Moruzzi", Università di Bologna, Via Belmeloro 8/2, 40126 Bologna, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Righi
authors.forename: Caterina
authors.initials: C
authors.lastname: Durante
authors.forename: Marina
authors.initials: M
authors.lastname: Cocchi
authors.forename: Carlo
authors.initials: C
authors.lastname: Calabrese
authors.forename: Giulio
authors.initials: G
authors.lastname: Di Febo
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Lecce
authors.forename: Annamaria
authors.initials: A
authors.lastname: Pisi
authors.forename: Vitaliano
authors.initials: V
authors.lastname: Tugnoli
authors.forename: Adele
authors.initials: A
authors.lastname: Mucci
authors.forename: Luisa
authors.initials: L
authors.lastname: Schenetti
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor