Preclinical animal model for de novo bone formation in human maxillary sinus.
OBJECTIVE
Up to now the effect of bone-substitute materials on de novo bone formation has been tested in a variety of preclinical animal models. We hypothesized that there is no significant difference in bone regeneration after application of autogenous bone (AB) and bone substitutes in a porcine calvarial monocortical defect model and in human maxillary sinus.


METHODS
Twenty-four weeks after application of ss-tricalcium phosphate (betaTCP), hydroxyapatite (HA), and AB in each of 3 defects on the sculls of 6 adult pigs (N = 54) and the application in 44 sinus floor elevations (11x betaTCP, 6x HA and 24x AB) in 41 patients, bone regeneration rates where compared microradiographically. Wilcoxon rank-sum test was used for statistical analysis.


RESULTS
Comparing the human with the animal specimens microradiographically, no significant difference of the mineralization rate could be found.


CONCLUSIONS
The chosen porcine model is a valuable method for preclinical testing of bone-substitute materials in maxillofacial surgery.
Schlegel
Karl Andreas
KA
Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
Rupprecht
Stephan
S
Petrovic
Ljubinko
L
Honert
Christian
C
Srour
Safwan
S
von Wilmowsky
Cornelius
C
Felszegy
Endre
E
Nkenke
Emeka
E
Lutz
Rainer
R
D000540:Alveolar Ridge Augmentation / Q000379:methods; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001847:Bone Diseases / Q000601:surgery; D001857:Bone Matrix / Q000637:transplantation; D001861:Bone Regeneration / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods*; D002113:Calcification, Physiologic / Q000502:physiology; D002130:Calcium Phosphates / Q000627:therapeutic use; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D004195:Disease Models, Animal; D017886:Durapatite / Q000627:therapeutic use; D005260:Female; D005624:Frontal Bone / Q000601:surgery; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008437:Maxilla / Q000601:surgery; D008443:Maxillary Sinus / Q000503:physiopathology / Q000601:surgery*; D008852:Microradiography; D008875:Middle Aged; D008903:Minerals / Q000627:therapeutic use; D010012:Osteogenesis / Q000502:physiology*; D013552:Swine
C077540:Bio-Oss; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D015921:Dental Implants; D008903:Minerals; C485817:beta-tricalcium phosphate; D017886:Durapatite