title: Two-dimensional LC-MS/MS determination of antiretroviral drugs in rat serum and urine.
abstract: A simple, rapid, reliable and highly sensitive on-line two-dimensional reversed-phase liquid chromatography-tandem mass spectrometric (2D-LC/MS/MS) method to determine antiretroviral drugs viz., abacavir (ABC), nevirapine (NVP) and indinavir (IDV) in rat serum and urine was developed and validated. The analytes were extracted on-line from rat serum and urine by a restricted access material (RAM) column and back-flushed into the reversed-phase C18 column for separation by LC. Detection was carried out by ESI-MS/MS. The developed method showed good selectivity, accuracy and precision for quantification of the antiretroviral drugs in rat serum and urine. Quantification limits for abacavir and nevirapine were 4.0 ng ml(-1), whereas for indinavir 4.7 ng ml(-1). The calibration graphs were linear in the range of 4-50 ng ml(-1)for abacavir, nevirapine and indinavir. The method was successfully applied to study the pharmacokinetics of antiretroviral in rats.
authors.affiliation: Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India. rnrao@iictnet.org
authors.forename: R
authors.initials: R
authors.lastname: Nageswara Rao
authors.forename: Dhananjay D
authors.initials: DD
authors.lastname: Shinde
mesh_terms: D000818:Animals; D044966:Anti-Retroviral Agents / Q000097:blood* / Q000652:urine*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D015224:Dideoxynucleosides / Q000097:blood / Q000652:urine; D016903:Drug Monitoring / Q000379:methods; D004867:Equipment Design; D008956:Models, Chemical; D019829:Nevirapine / Q000097:blood / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D000099227:Abacavir
chemical_list: D044966:Anti-Retroviral Agents; D015224:Dideoxynucleosides; D019829:Nevirapine; D000099227:Abacavir