[Effect of RNA interference targeting-survivin on the invasiveness of human glioma cells in vitro].
OBJECTIVE
To study the role of survivin gene in the invasive behavior of glioma cells and explore the possible mechanism.


METHODS
The mRNA and protein expressions of survivin in glioma cell line SNB19 transfected by small interfering RNA (siRNA) targeting survivin were determined by real time RT-PCR and Western blotting, respectively. The anchorage-independent growth of the cells was examined by clone formation assay in soft agar, and their invasiveness was evaluated using a Boyden chamber model. The protein level of urokinase-type plasminogen activator (uPA) was also determined by western blotting.


RESULTS
Survivin siRNA dose-dependently inhibited the anchorage-independent growth and invasiveness and reduced the expression of uPA protein in SNB19 cells.


CONCLUSIONS
RNA interference targeting survivin can inhibit the invasiveness of glioma cells in vitro possibly by down-regulating uPA expression.
Shi
De-Gang
DG
Cancer Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China. shidegang@tom.com
Fan
Yu
Y
Zhu
Fu
F
Zhang
Ji-Ren
JR
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D000077022:Survivin; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics / Q000378:metabolism
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000077022:Survivin; D014568:Urokinase-Type Plasminogen Activator