title: Ectopic overproduction of a sporulation-specific transcription factor induces assembly of prespore-like membranous compartments in vegetative cells of fission yeast.
abstract: Mei4 is a key sporulation-specific transcription factor in fission yeast. Ectopic expression of Mei4 in vegetative cells caused formation of nucleated membranous compartments, which shared common features with normal forespore membranes, thereby perturbing nuclear division. These results suggest why expression of development-specific transcription factors must be strictly controlled.
authors.affiliation: Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakase
authors.forename: Aiko
authors.initials: A
authors.lastname: Hirata
authors.forename: Chikashi
authors.initials: C
authors.lastname: Shimoda
authors.forename: Taro
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D015153:Blotting, Western; D002451:Cell Compartmentation; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009154:Mutation; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D013172:Spores, Fungal / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
chemical_list: D029702:Schizosaccharomyces pombe Proteins; C533268:Spo13 protein, S pombe; C489539:mei4 protein, S pombe; D049452:Green Fluorescent Proteins