Demonstration of persistent strains of Campylobacter jejuni within broiler farms over a 1-year period in Lithuania.
OBJECTIVE
The aim of the study was to investigate the flock prevalence of Campylobacter jejuni and Campylobacter coli in broiler farms in Lithuania and to identify possible persistent strains of Camp. jejuni using amplified fragment length polymorphism (AFLP) typing method.


RESULTS
During 1 year, 42 broiler flocks from 9 broiler farms were examined to determine the prevalence of Campylobacter-positive broiler flocks in Lithuania. Among 42 broiler flocks examined, 31 flocks (73.8%) were positive for Camp. jejuni and 17 flocks (40.48%) for Camp. coli. Campylobacter jejuni isolates were genotyped by AFLP method using BspDI and BglII restriction enzymes. Typing of 190 isolates generated 50 AFLP genotypes with the highest diversity of strains found in the summer season. Each farm showed one or more predominant AFLP types, and one AFLP type (A32) was found in five broiler farms over a 1-year period.


CONCLUSIONS
Campylobacter jejuni and Camp. coli are highly prevalent in broiler farms in Lithuania. Farm-specific genotypes were identified in all farms examined. Type A32 was present and persisted in different broiler farms, and a common source of transmission of Camp. jejuni was suspected.


CONCLUSIONS
For the first time, Camp. jejuni in broiler flocks has been genetically characterized in Lithuania. Persistent strains of Camp. jejuni were detected over one period at the beginning of broiler meat production chain and, therefore, the identification of contamination source of such strains and the mechanism of their particular ability to persist are crucial to establish effective control measures against Camp. jejuni infection in broiler farms.
Kudirkienė
E
E
 Department of Food Safety and Animal Hygiene, Faculty of Veterinary Medicine, Lithuanian Veterinary Academy, Kaunas, Lithuania.
Malakauskas
M
M
 Department of Food Safety and Animal Hygiene, Faculty of Veterinary Medicine, Lithuanian Veterinary Academy, Kaunas, Lithuania.
Malakauskas
A
A
 Department of Infectious Diseases, Faculty of Veterinary Medicine, Lithuanian Veterinary Academy, Kaunas, Lithuania.
Bojesen
A M
AM
 Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark.
Olsen
J E
JE
 Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark.
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D015373:Bacterial Typing Techniques; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D008097:Lithuania; D015203:Reproducibility of Results
D004269:DNA, Bacterial