title: [Oxidative stress markers in atherogenesis induced by hyperfibrinogenemia].
abstract: BACKGROUND
  We studied plasmatic TNF-alpha, nitric oxide (NO) and citrulline behaviors and probable morphological mitochondrial alterations in aortic smooth muscle cells, in rats with atherogenesis induced by hyperfibrinogenemia in: A) control, B) multiple injured for 30 days and C) multiple injured for 60 days.
  
  
  METHODS
  Hyperfibrinogenemia induction: adrenaline injection (0,1 mg/rat/day). TNF-alpha (pg/dL) was determined by Elisa and NO (microM) and citrulline (mM) by spectrophotometry. Morphological mitochondrial alterations were studied by electronic microscopy. Variables were analized: ANOVA, r coefficient and chi2 test.
  
  
  RESULTS
  We observed a significant increment of TNF-alpha in multiple injured for 30 days (B) (50.05 +/- 2.29) as well as in multiple injured for 60 days (C) (74.99 +/- 2.82) related to control (A) (33.01 +/- 1.49) (p<0.001 in both groups). Citrulline presented a significant increased in (B) (5.56 +/- 0.20) and (C) (6.84 +/- 0.13) when compared to (A) (4.41 +/- 0.23) (p<0.001 in both situations). Mean while NO biodisponibility diminished significantly in (B) (8.97 +/- 0.70) and in (C) (5.32 +/- 0.68) when compared to (A) (21.65 +/- 1.74) (p<0.001 in both situations).
  
  
  CONCLUSIONS
  Hyperfibrinogenemia could modify the NO physiopathological pathway and produced morphological mitochondrial alterations in aortic smooth muscle cells, probably producing ischemic lesions in the vascular wall and altering the vasodilatation response.
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional Córdoba, Argentina.
authors.forename: María C
authors.initials: MC
authors.lastname: Báez
authors.forename: Mariana D
authors.initials: MD
authors.lastname: Táran
authors.forename: Vilma
authors.initials: V
authors.lastname: Campana
authors.forename: Juan C
authors.initials: JC
authors.lastname: Simes
authors.forename: Patricia
authors.initials: P
authors.lastname: Pons
authors.forename: José
authors.initials: J
authors.lastname: Atilio-Palma
authors.forename: Mónica
authors.initials: M
authors.lastname: Moya
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000209:etiology* / Q000473:pathology; D015415:Biomarkers / Q000097:blood; D002956:Citrulline / Q000097:blood*; D004285:Dogs; D005340:Fibrinogen / Q000032:analysis*; D008659:Metabolic Diseases / Q000097:blood*; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha; D002956:Citrulline; D009569:Nitric Oxide; D005340:Fibrinogen