title: Phellinins A1 and A2, new styrylpyrones from the culture broth of Phellinus sp. KACC93057P: I. Fermentation, taxonomy, isolation and biological properties.
abstract: Novel styrylpyrones, phellinins A1 and A2, were isolated together with known styrylpyrone compounds, hispidin and 1,1-distyrylpyrylethan, from the cultured broth of Phellinus sp. KACC93057P. These compounds were purified by solvent partition, Sephadex LH-20 column chromatography, C(18)-solid phase extraction and finally by reversed-phase (ODS) TLC. To identify the phellinin producer Phellinus sp. KACC93057P, the ribosomal DNA (rDNA) internal transcribed space regions containing 5.8 rDNA were sequenced and compared with those of the known Phellinus isolates. Phellinus sp. KACC93057P was 94.8% identical to P. baumii and P. linteus, all of which did not produce phellinins A1 and A2. These compounds significantly scavenged free radicals such as 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and superoxide.
authors.affiliation: Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, Korea.
authors.forename: In-Kyoung
authors.initials: IK
authors.lastname: Lee
authors.forename: Geon-Sik
authors.initials: GS
authors.lastname: Seo
authors.forename: Nak Beom
authors.initials: NB
authors.lastname: Jeon
authors.forename: Hee-Wan
authors.initials: HW
authors.lastname: Kang
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D011753:Pyrones / Q000302:isolation & purification* / Q000494:pharmacology; D013343:Styrenes / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D011753:Pyrones; D013343:Styrenes; C545889:phellinin A1; C545890:phellinin A2