title: Cocaine effects on dopamine and NMDA receptors interactions in the striatum of Fischer rats.
abstract: Numerous studies have shown that biochemical and behavioral effects of cocaine are mediated by dopamine D1 receptor (D1R) and NMDA R1 receptor (NR1)-mediated transmission. In this study, we investigated the physical interactions between D1R and NR1 in response to acute cocaine administration in a time course of 5-60 min. In the caudate-putamen (CPu) of male Fischer rats, a single cocaine injection (30 mg/kg) reduced D1R-NR1 protein-protein interactions 30 min after treatment. In addition, activation or blockade of the NMDA receptor using NMDA (25mg/kg) or MK-801 (0.25mg/kg), respectively, also reduced the D1R-NR1 physical interactions. Acute cocaine administration did not alter total D1R or NR1 protein levels in our time course of study. These results indicate that D1R-NR1 physical interaction rather than total protein levels may regulate the intracellular signaling after acute cocaine administration.
authors.affiliation: Department of Psychology, Hunter College, CUNY, 695 Park Ave., New York, NY 10065, USA. wsun@hunter.cuny.edu
authors.forename: Wei-Lun
authors.initials: WL
authors.lastname: Sun
authors.forename: Luyi
authors.initials: L
authors.lastname: Zhou
authors.forename: Vanya
authors.initials: V
authors.lastname: Quinones-Jenab
authors.forename: Shirzad
authors.initials: S
authors.lastname: Jenab
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002421:Caudate Nucleus / Q000187:drug effects* / Q000378:metabolism; D003042:Cocaine / Q000494:pharmacology*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D047468:Immunoprecipitation; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology; D011699:Putamen / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D017447:Receptors, Dopamine D1 / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: D018765:Dopamine Uptake Inhibitors; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; C409466:NR1 NMDA receptor; D017447:Receptors, Dopamine D1; D016194:Receptors, N-Methyl-D-Aspartate; D014404:Tubulin; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D003042:Cocaine