Phosphoenolpyruvate-dependent phosphorylation of sucrose by Clostridium tyrobutyricum ZJU 8235: evidence for the phosphotransferase transport system.
The uptake and metabolism of sucrose, the major sugar in industrial cane molasses, by Clostridium tyrobutyricum ZJU 8235 was investigated and this study provided the first definitive evidence for phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity in butyric acid-producing bacteria. Glucose was utilized preferentially to sucrose when both substrates were present in the medium. The PEP-dependent sucrose: PTS was induced by growing C. tyrobutyricum on sucrose (but not glucose) as the sole carbon source. Extract fractionation and PTS reconstitution experiments revealed that both soluble and membrane components were required for bioactivity. Sucrose-6-phosphate hydrolase and fructokinase activities were also detected in sucrose-grown cultures. Based on these findings, a pathway of sucrose metabolism in this organism was proposed that includes the forming of sucrose-6-phosphate via the PTS and its further degradation into glucose-6-phosphate and fructose-6-phosphate.
Jiang
Ling
L
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
Cai
Jin
J
Wang
Jufang
J
Liang
Shizhong
S
Xu
Zhinan
Z
Yang
Shang-Tian
ST
D001693:Biological Transport, Active; D048014:Clostridium tyrobutyricum / Q000145:classification* / Q000378:metabolism*; D004789:Enzyme Activation; D008954:Models, Biological; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D044345:Phosphoric Triester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism*
D013395:Sucrose; D010728:Phosphoenolpyruvate; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D044345:Phosphoric Triester Hydrolases