title: Interactions of zoospores of Ulva linza with arginine-rich oligopeptide monolayers.
abstract: We recently reported on the strong interactions of zoospores of the green alga, Ulva linza with an arginine-rich oligopeptide self-assembled monolayer (SAM) [Biofouling 2008, 24, 303-312], where the arginine-rich peptide induced not only high spore settlement, but also a form of abnormal settlement, or "pseudo-settlement", whereby a proportion of spores do not go through the normal process of surface exploration, adhesive exocytosis, and loss of flagella. Further, it was demonstrated that both the total number of settled spores and the fraction of pseudosettled spores were related to the surface density of the arginine-rich peptide. Here we present a further investigation of the interactions of zoospores of Ulva with a set of oligomeric, de novo designed, arginine-rich peptides, specifically aimed to test the effect of peptide primary structure on the interaction. Via variations in the peptide length and by permutations in the amino acid sequences, we gain further insight into the spore-surface interactions. The interpretation of the biological assays is supported by physicochemical characterization of the SAMs using infrared spectroscopy, ellipsometry, and contact angle measurements. Results confirm the importance of arginine residues for the anomalous pseudosettlement, and we found that settlement is modulated by variations in both the total length and peptide primary structure. To elucidate the causes of the anomalous settlement and the possible relation to peptide-membrane interactions, we also compared the settlement of the "naked" zoospores of Ulva (which present a lipoprotein membrane to the exterior without a discrete polysaccharide cell wall), with the settlement of diatoms (unicellular algae that are surrounded by a silica cell wall), onto the peptide SAMs. Cationic SAMs do not notably affect settlement (attachment), adhesion strength, or viability of diatom cells, suggesting that the effect of the peptides on zoospores of Ulva is mediated via specific peptide-membrane interactions.
authors.affiliation: Division of Molecular Physics, Department of Physics, Chemistry and Biology, Linköpings Universitet, Linköping, Sweden. ted@ifm.liu.se
authors.forename: T
authors.initials: T
authors.lastname: Ederth
authors.forename: M E
authors.initials: ME
authors.lastname: Pettitt
authors.forename: P
authors.initials: P
authors.lastname: Nygren
authors.forename: C-X
authors.initials: CX
authors.lastname: Du
authors.forename: T
authors.initials: T
authors.lastname: Ekblad
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Falk
authors.forename: M E
authors.initials: ME
authors.lastname: Callow
authors.forename: J A
authors.initials: JA
authors.lastname: Callow
authors.forename: B
authors.initials: B
authors.lastname: Liedberg
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D013170:Spores / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D044445:Ulva / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D001120:Arginine