title: In vitro proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells cultured with hardystonite (Ca2ZnSi 2O7) and {beta}-TCP ceramics.
abstract: The effects of hardystonite (Ca(2)ZnSi(2)O(7), CSZn) and tricalcium phosphate (beta-TCP) on the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) were compared by directly culturing MSCs on ceramic disks (contact mode) or separately culturing cells with ceramic disks (non-contact mode). In non-contact mode, the CSZn ceramic supported MSC proliferation more strongly than did the beta-TCP ceramic. However, in contact mode, the MSCs proliferated more quickly on the beta-TCP ceramic than they did on the CSZn ceramic. Alkaline phosphatase (ALP) staining and osteogenic gene expression analysis showed that the CSZn and beta-TCP ceramics had significant effects on the promotion of the osteogenic differentiation of MSCs in both non-contact and contact mode. Furthermore, in contact mode, the CSZn disk promoted the osteogenic differentiation of MSCs more strongly than did the beta-TCP disks. Even without the induction of dexamethasone and beta-glycerophosphate, CSZn stimulated the osteogenic differentiation of MSCs. These results suggest that CSZn ceramic would be a useful candidate material for bone regeneration and hard tissue engineering.
authors.affiliation: University of Tsukuba, Ibaraki, Japan.
authors.forename: Hongxu
authors.initials: H
authors.lastname: Lu
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawazoe
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tateishi
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Jin
authors.forename: Jiang
authors.initials: J
authors.lastname: Chang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002516:Ceramics; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D016133:Polymerase Chain Reaction; D017640:Silicates / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D017640:Silicates; C000617419:hardystonite; D000469:Alkaline Phosphatase