[Immunohistochemical study of TNF-alpha expression in the kidney of rats with lipopolysaccharide and heat stresses].
OBJECTIVE
To investigate the effects of co-exposure of LPS and heat on TNF-alpha expression in rat kidneys.


METHODS
Male pathogen-free Wistar rats were randomly assigned in saline-injected normothermic control (C group), saline-injected heat exposure (H group), LPS-injected normothermic control (L group), and LPS-injected heat exposure (HL group). The rats in H and HL groups were exposed in a chamber at an ambient dry bulb temperature (Tdb) of 35.0-/+0.5 degrees, and those in C and L groups were exposed to a Tdb of 26-/+0.5 degrees. The rats in L and HL groups were given an intravenous injection of LPS (10 mg/kg) via the tail vein to induce endotoxemia, and equivalent normal saline was injected in C and H groups. TNF-alpha expression in the kidney was detected by immunohistochemical SABC method, and the renal damage was evaluated histologically at 120 min after the treatment.


RESULTS
Co-exposure of the rats with LPS and heat caused significantly enhanced TNF-alpha expression and histopathological damage in the kidneys.


CONCLUSIONS
LPS combined with heat exposure causes renal toxicity, while is closely associated with the expression of TNF-alpha in the kidneys.
Zuo
Dan
D
Department of Orthopedics, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. zuodan1977@hotmail.com
Zhou
Li-ning
LN
Xie
Feng
F
He
Dan-dan
DD
Mei
Gui-ping
GP
D000818:Animals; D005786:Gene Expression Regulation; D006358:Hot Temperature; D007150:Immunohistochemistry; D007378:Interleukins / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007378:Interleukins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha