The OsDHODH1 gene is involved in salt and drought tolerance in rice.
In the present paper, we identified and cloned OsDHODH1 encoding a putative cytosolic dihydroorotate dehydrogenase (DHODH) in rice. Expression analysis indicated that OsDHODH1 is upregulated by salt, drought and exogenous abscisic acid (ABA), but not by cold. By prokaryotic expression, we determined the enzymatic activity of OsDHODH1 and found that overproduction of OsDHODH1 significantly improved the tolerance of Escherichia coli cells to salt and osmotic stresses. Overexpression of the OsDHODH1 gene in rice increased the DHODH activity and enhanced plant tolerance to salt and drought stresses as compared with wild type and OsDHODH1-antisense transgenic plants. Our findings reveal, for the first time, that cytosolic dihydroorotate dehydrogenase is involved in plant stress response and that OsDHODH1 could be used in engineering crop plants with enhanced tolerance to salt and drought.
Liu
Wen-Ying
WY
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Wang
Mei-Mei
MM
Huang
Ji
J
Tang
Hai-Juan
HJ
Lan
Hong-Xia
HX
Zhang
Hong-Sheng
HS
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D002734:Chlorophyll / Q000378:metabolism; D000090722:Dihydroorotate Dehydrogenase; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D016877:Oxidants / Q000494:pharmacology; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D011392:Proline / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature
D000090722:Dihydroorotate Dehydrogenase; D016877:Oxidants; D010937:Plant Growth Regulators; D010940:Plant Proteins; D002734:Chlorophyll; D012965:Sodium Chloride; D000040:Abscisic Acid; D011392:Proline; D006861:Hydrogen Peroxide; D044925:Oxidoreductases Acting on CH-CH Group Donors