title: The mechanism of butyrate-induced collagen biosynthesis in cultured fibroblasts.
abstract: The data showing that butyrate may play an important role in cellular metabolism led us to study its effect on collagen biosynthesis in cultured fibroblasts. Since insulin-like growth factor-I (IGF-I) is the most potent stimulator of collagen biosynthesis in fibroblasts, the effect of butyrate on IGF-I receptor (IGF-IR) expression was evaluated. Confluent human dermal fibroblasts were treated with millimolar concentrations of sodium butyrate (NaB) for 48 hours. It was found that butyrate induced collagen biosynthesis and prolidase activity. It was found that the exposure of the cells to 4 mM butyrate contributed to a distinct increase in IGF-IR. It was accompanied by a parallel increase in the expression of SOS protein and MAP-kinases (ERK1, ERK2). It was found that the MEK inhibitor decreased collagen biosynthesis and expression of MAP-kinases (ERK1, ERK2), while NaB counteracted the process. The data suggest that butyrate-dependent stimulation of collagen biosynthesis in cultured human skin fibroblasts undergoes through IGF-IR signaling.
authors.affiliation: Department of Medicinal Chemistry, Medical University in Białystok, Kilińskiego 1, 15-089 Białystok, Poland. ewakarna@umwb.edu.pl
authors.forename: Ewa
authors.initials: E
authors.lastname: Karna
authors.forename: Sylwia
authors.initials: S
authors.lastname: Trojan
authors.forename: Jerzy A
authors.initials: JA
authors.lastname: Pałka
mesh_terms: D015153:Blotting, Western; D002087:Butyrates / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004150:Dipeptidases / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D019950:Mitogen-Activated Protein Kinase 1 / Q000737:chemistry; D017526:Receptor, IGF Type 1 / Q000187:drug effects; D020839:Son of Sevenless Protein, Drosophila / Q000737:chemistry
chemical_list: D002087:Butyrates; D007202:Indicators and Reagents; D020839:Son of Sevenless Protein, Drosophila; D003094:Collagen; D017526:Receptor, IGF Type 1; D019950:Mitogen-Activated Protein Kinase 1; D004150:Dipeptidases; C022449:proline dipeptidase