title: Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways.
abstract: We investigated the mechanism by which equine herpesvirus-1 (EHV-1) enters primary cultured equine brain microvascular endothelial cells (EBMECs) and equine dermis (E. Derm) cells. EHV-1 colocalized with caveolin in EBMECs and the infection was greatly reduced by the expression of a dominant negative form of equine caveolin-1 (ecavY14F), suggesting that EHV-1 enters EBMECs via caveolar endocytosis. EHV-1 entry into E. Derm cells was significantly reduced by ATP depletion and treatments with lysosomotropic agents. Enveloped virions were detected from E. Derm cells by infectious virus recovery assay after viral internalization, suggesting that EHV-1 enters E. Derm cells via energy- and pH-dependent endocytosis. These results suggest that EHV-1 utilizes multiple endocytic pathways in different cell types to establish productive infection.
authors.affiliation: Laboratory of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, West 9 North 18, Kita-ku, Sapporo 060-0818, Japan. r-hasebe@vetmed.hokudai.ac.jp
authors.forename: Rie
authors.initials: R
authors.lastname: Hasebe
authors.forename: Michihito
authors.initials: M
authors.lastname: Sasaki
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Wada
authors.forename: Takashi
authors.initials: T
authors.lastname: Umemura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004705:Endocytosis; D042783:Endothelial Cells / Q000821:virology; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000254:growth & development / Q000502:physiology*; D006734:Horse Diseases / Q000821:virology; D006736:Horses; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D019076:Transgenes; D053586:Virus Internalization
chemical_list: D051242:Caveolin 1; D011505:Protein-Tyrosine Kinases