Colonisation of poplar trees by gfp expressing bacterial endophytes.
With the exception of nitrogen fixing bacteria, there is little known about the colonisation patterns or population sizes of bacterial endophytes in deciduous trees. This study describes the isolation, identification, construction and re-colonisation patterns of three green fluorescent protein(gfp):kanamycin(R) labelled bacterial endophytes when re-introduced into poplar trees, their original host plant. Two of these endophytes showed considerable colonisation in the roots and stems of inoculated plants. gfp expressing cells of all three strains were observed to colonise the xylem tissue of the root. All three strains proved to be efficient rhizosphere colonisers, supporting the theory that the rhizosphere can serve as a source of bacterial endophytes.
Germaine
Kieran
K
Department of Applied Biology and Chemistry, Institute of Technology, Carlow, Ireland.
Keogh
Elaine
E
Garcia-Cabellos
Guiomar
G
Borremans
Brigitte
B
Lelie
Daniel
D
Barac
Tanja
T
Oeyen
Licy
L
Vangronsveld
Jaco
J
Moore
Fiona Porteous
FP
Moore
Edward R B
ER
Campbell
Colin D
CD
Ryan
David
D
Dowling
David N
DN
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D044822:Biodiversity; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007613:Kanamycin Resistance; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013194:Staining and Labeling / Q000379:methods; D052584:Xylem / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins