title: Freeze-thawing as the factor of spontaneous activation of spermatozoa motility in common carp (Cyprinus carpio L.).
abstract: In the present study, we investigated the possibility of spontaneous carp spermatozoa activation by freeze-thawing. To evaluate this, the parameters of spermatozoa motility percentage, velocity, ATP content level and fertility rate of sperm were used. The motility and velocity of spermatozoa activated by freeze-thawing were characterized by motile spermatozoa with a median value of 16% and a velocity of 98 microm/s. In addition, the motility and velocity of sperm from the thawed samples were significantly lower than in the control (median value of 100% for sperm motility and 175 microm/s for sperm velocity). Furthermore, a spontaneously activated spermatozoa motility terminated within five minutes post-thaw time. After freeze-thawing the ATP level significantly decreased with post-thaw time (46 nmol ATP/10(9) and 10 nmol ATP/10(9) at 25s and 10 min after thawing, respectively). Fertility of spermatozoa was not significantly affected within 10 min post-thaw. On the other hand, the fertility of frozen-thawed sperm was significantly lower if compared to fresh sperm. We conclude that the freeze-thawing procedure spontaneously activated spermatozoa motility in common carp. However, this activation did not negatively affect the fertility of frozen-thawed sperm.
authors.affiliation: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic. boryss00@vurh.jcu.cz
authors.forename: S
authors.initials: S
authors.lastname: Boryshpolets
authors.forename: B
authors.initials: B
authors.lastname: Dzyuba
authors.forename: M
authors.initials: M
authors.lastname: Rodina
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Hulak
authors.forename: D
authors.initials: D
authors.lastname: Gela
authors.forename: O
authors.initials: O
authors.lastname: Linhart
mesh_terms: D000255:Adenosine Triphosphate; D000818:Animals; D002347:Carps; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary*; D005615:Freezing; D008297:Male; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D000255:Adenosine Triphosphate