title: A method for nanofluidic device prototyping using elastomeric collapse.
abstract: Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques are limited by the complexity of the devices that can be fabricated, which can only create straight or irregular channels normal to the direction of an applied strain. Here, we report a technique for nanofluidic fabrication based on the controlled collapse of microchannel structures. As is demonstrated, this method converts the easy to control vertical dimension of a PDMS mold to the lateral dimension of a nanochannel. We demonstrate here the creation of complex nanochannel structures as small as 60 nm and provide simple design rules for determining the conditions under which nanochannel formation will occur. The applicability of the technique to biomolecular analysis is demonstrated by showing DNA elongation in a nanochannel and a technique for optofluidic surface enhanced Raman detection of nucleic acids.
authors.affiliation: School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Seung-min
authors.initials: SM
authors.lastname: Park
authors.forename: Yun Suk
authors.initials: YS
authors.lastname: Huh
authors.forename: Harold G
authors.initials: HG
authors.lastname: Craighead
authors.forename: David
authors.initials: D
authors.lastname: Erickson
mesh_terms: D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D003716:Dengue Virus / Q000235:genetics; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000648:ultrastructure; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012826:Silicone Elastomers; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D004279:DNA, Viral; D004129:Dimethylpolysiloxanes; D012367:RNA, Viral; D012826:Silicone Elastomers; C013830:baysilon