title: A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae.
abstract: We report a multiplatform real-time polymerase chain reaction methodology based on genes encoding for the regulatory toxR activator and enterotoxin A protein to determine enterotoxigenic Vibrio cholerae types from other vibrios. This assay, which was successfully validated on a collection of 87 bacterial strains, including 63 representatives of V. cholerae and 8 noncholera vibrios provides a rapid tool for detection and identification of cholera.
authors.affiliation: Centres for Military Medicine and for Biological Threat Preparedness, FI-00290 Helsinki, Finland.
authors.forename: Katja A
authors.initials: KA
authors.lastname: Koskela
authors.forename: Pirjo
authors.initials: P
authors.lastname: Matero
authors.forename: Janet M
authors.initials: JM
authors.lastname: Blatny
authors.forename: Else M
authors.initials: EM
authors.lastname: Fykse
authors.forename: Jaran Strand
authors.initials: JS
authors.lastname: Olsen
authors.forename: Lasse O
authors.initials: LO
authors.lastname: Nuotio
authors.forename: Simo
authors.initials: S
authors.lastname: Nikkari
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D002771:Cholera / Q000175:diagnosis / Q000382:microbiology; D004268:DNA-Binding Proteins / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics; D004783:Environmental Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004268:DNA-Binding Proteins; D004768:Enterotoxins; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae