title: Glutathione disulfide and S-nitrosoglutathione detoxification by Mycobacterium tuberculosis thioredoxin system.
abstract: Mycobacterium tuberculosis resides within alveolar macrophages. These phagocytes produce reactive nitrogen and oxygen intermediates to combat the invading pathogens. The macrophage glutathione (GSH) pool reduces nitric oxide (NO) to S-nitrosoglutathione (GSNO). Both glutathione disulfide (GSSG) and GSNO possess mycobactericidal activities in vitro. In this study we demonstrate that M. tuberculosis thioredoxin system, comprises of thioredoxin reductase B2 and thioredoxin C reduces the oxidized form of the intracellular mycothiol (MSSM) and is able to efficiently reduce GSSG and GSNO in vitro. Our study suggests that the thioredoxin system provide a general reduction mechanism to cope with oxidative stress associated with the microbe's metabolism as well as to detoxify xenobiotics produced by the host.
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, British Columbia, Canada V5Z 3J5.
authors.forename: Rodgoun
authors.initials: R
authors.lastname: Attarian
authors.forename: Chelsea
authors.initials: C
authors.lastname: Bennie
authors.forename: Horacio
authors.initials: H
authors.lastname: Bach
authors.forename: Yossef
authors.initials: Y
authors.lastname: Av-Gay
mesh_terms: D019803:Glutathione Disulfide / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000378:metabolism*; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D013879:Thioredoxins; D026422:S-Nitrosoglutathione; D013880:Thioredoxin-Disulfide Reductase; D019803:Glutathione Disulfide