Molecular characterization of flavonoid malonyltransferase from Oryza sativa.
In this study, a flavonoid malonyltransferase (OsMaT-2) was cloned from Oryza sativa, and the recombinant protein OsMaT-2 was purified via affinity chromatography. OsMaT-2 utilized a variety of flavonoid glucosides, including flavanone glucosides, flavone glucosides, flavonol glucosides, and isoflavone glucosides as substrates, but did not utilize anthocyanin. As an acyl donor, OsMaT-2 utilized only malonyl-CoA. Based on reactions with various quercetin 3-O-sugars, we identified the probable position of malonylation as the 6''-hydroxyl group of the sugar. This is the first report, to the best of our knowledge, of the cloning of a flavonoid malonyltransferase from O. sativa.
Kim
Dea Hwan
DH
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Republic of Korea.
Kim
Seong Kyong
SK
Kim
Jeong-Ho
JH
Kim
Bong-Gyu
BG
Ahn
Joong-Hoon
JH
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D002241:Carbohydrates; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant; D008316:Malonyl Coenzyme A; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011794:Quercetin; D018749:RNA, Plant / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D002241:Carbohydrates; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant; D011994:Recombinant Proteins; D008316:Malonyl Coenzyme A; D011794:Quercetin; D000217:Acyltransferases