title: [Effects and mechanism of decorin on the proliferation of HuH7 hepatoma carcinoma cells in vitro].
abstract: OBJECTIVE
  To investigate the effects and mechanism of decorin( DCN) on the proliferation of HuH7 hepatoma carcinoma cell line in vitro.
  
  
  METHODS
  Hepatoma carcinoma cells was cultured with DCN in different concentration (0, 25, 50, 75, 100, 125, 150, 200 microg/L) for different time(12, 24, 48, 72 h and 2 weeks). Cell activities were studied by MTT and clone test. The changes of cell cycle and apoptosis were analyzed by Flow cytometry.
  
  
  RESULTS
  The proliferation of HuH7 cells could be inhibited by DCN in vitro and the inhibition effect was the time and dose dependent relationship. DCN could block cell cycle at G(1); phase. Apoptosis of hepatocarcinoma cells could be efficiently induced by DCN in a time/dose-dependent manner.
  
  
  CONCLUSIONS
  DCN may be a negative regulatory protein inhibiting hepatoma carcinoma cell proliferation through inhibiting cell cycle and inducing apoptosis of cell in vitro.
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jian-ying
authors.initials: JY
authors.lastname: Shangguan
authors.forename: Ke-feng
authors.initials: KF
authors.lastname: Dou
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Hu
authors.forename: Fu-qin
authors.initials: FQ
authors.lastname: Zhang
authors.forename: Zhao-sheng
authors.initials: ZS
authors.lastname: Yong
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Ti
mesh_terms: D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000503:physiopathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D011509:Proteoglycans / Q000378:metabolism*
chemical_list: C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans