title: The presence of Saccharomyces cerevisiae DNA in various media used to propagate yeasts and its removal by ethidium monoazide.
abstract: OBJECTIVE
  In this study we demonstrate the interference of yeast extract in enumeration of Saccharomyces cerevisiae using real-time PCR and develop a method for its removal from the media using ethidium monoazide (EMA).
  
  
  RESULTS
  Using real-time PCR and primers to S. cerevisiae we demonstrate the presence of yeast DNA in various media as well as the media impact on S. cerevisiae real-time PCR standard curves. By pretreatment with EMA, we were able to remove this interference.
  
  
  CONCLUSIONS
  Saccharomyces cerevisiae DNA can be found in a number of common laboratory media and may impact the enumeration of this yeast by real-time PCR. However, pretreatment with EMA eliminates this concern.
  
  
  CONCLUSIONS
  We have developed a method for removal of contaminating DNA in yeast growth media.
authors.affiliation: Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA, USA.
authors.forename: H
authors.initials: H
authors.lastname: Rawsthorne
authors.forename: T G
authors.initials: TG
authors.lastname: Phister
mesh_terms: D001386:Azides / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015003:Yeasts / Q000254:growth & development*
chemical_list: D001386:Azides; D003470:Culture Media; D004271:DNA, Fungal; C014535:8-azidoethidium