Relations between open-field, elevated plus-maze, and emergence tests in C57BL/6J and BALB/c mice injected with GABA- and 5HT-anxiolytic agents.
Two 5HT(1A) receptor agonists and chlordiazepoxide were examined in open-field, elevated plus maze, and emergence tests. At doses with no effect in the open-field, chlordiazepoxide increased open and open/total arm visits as well as open arm duration in the elevated plus maze, whereas 5HT(1A) receptor agonists showed an anxiolytic response on a single measure. The anxiolytic action of chlordiazepoxide was limited to the less active BALB/c strain. Unlike the 5HT(1A) receptor agonists, chlordiazepoxide was also anxiolytic in the emergence test, once again only in BALB/c and not C57BL/6J mice. Significant correlations were found between emergence latencies and specific indicators of anxiety in the elevated plus-maze in chlordiazepoxide-treated but not in mice treated with buspirone and 8-OH-DPAT. These results indicate that elevated plus-maze and emergence tests depend on benzodiazepine receptors. In contrast, 5HT(1A) receptor agonists were ineffective in the emergence test and no correlation was found between emergence latencies and specific indicators of anxiety in the elevated plus-maze. Though superficially similar, the emergence test seems to tap into a partially separate facet of anxiety.
Lalonde
Robert
R
Centre Hospitalier de l'Université de Montréal/St-Luc, Unité de Recherche en Sciences Neurologiques, Montréal, Québec, Canada H2X 3J4. robert.lalonde@umontreal.ca
Strazielle
Catherine
C
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology* / Q000633:toxicity; D001007:Anxiety / Q000139:chemically induced* / Q000378:metabolism / Q000523:psychology; D002707:Chlordiazepoxide / Q000494:pharmacology / Q000633:toxicity; D005106:Exploratory Behavior / Q000187:drug effects* / Q000502:physiology; D018757:GABA Modulators / Q000494:pharmacology* / Q000633:toxicity; D018782:Maze Learning / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017366:Serotonin Receptor Agonists / Q000494:pharmacology* / Q000633:toxicity; D013045:Species Specificity
D014151:Anti-Anxiety Agents; D018757:GABA Modulators; D017366:Serotonin Receptor Agonists; D002707:Chlordiazepoxide