Single cell protein production from yacon extract using a highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a and its nutritive analysis.
The intracellular protein in the highly thermosensitive and permeable mutant can be easily released when they are incubated both in the low-osmolarity water and at the non-permissive temperature (usually 37 degrees C). After the mutant was grown in the yacon extract for 45 h, the crude protein content in the highly thermosensitive and permeable mutant Z114 was 59.1% and over 61% of the total protein could be released from the cells treated at 37 degrees C. The mutant cells grown in the yacon extract still contained high level of essential amino acids and other nutrients. This means that the yacon extract could be used as the medium for growth of the highly thermosensitive and permeable mutant which contained high content of crude protein.
Zhao
Chun-Hai
CH
Key Laboratory of Marine Genetics, Gene Resource and Exploitation of Ministry of Education, Ocean University of China, Qingdao, China.
Zhang
Tong
T
Chi
Zhen-Ming
ZM
Chi
Zhe
Z
Li
Jing
J
Wang
Xiang-Hong
XH
D003454:Cryptococcus / Q000235:genetics / Q000254:growth & development*; D005656:Fungal Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D009154:Mutation; D010539:Permeability; D010936:Plant Extracts / Q000737:chemistry*
D005656:Fungal Proteins; D010936:Plant Extracts; C535244:yacon syrup