title: The effects of prolactin and corticosterone on insulin binding to rat Leydig cells.
abstract: The influence of prolactin (PRL) and corticosterone on insulin binding to purified rat Leydig cells was assessed in vitro. The lowest dose of PRL (50 ng/ml) increased (p<0.05) and the remaining PRL concentrations (100, 150, 200, 250 ng/ml) decreased (p<0.05) the insulin binding to Leydig cells. All doses of corticosterone (150, 200, 250, 300 ng/ml) except the lowest one (100 ng/ml) decreased the insulin binding. In conclusion, hyperprolactinemia or excess glucocorticoids associated with an impairment of testicular steroidogenesis may be mediated by a defective insulin binding to Leydig cells.
authors.affiliation: Reproductive Medicine Unit, Department of Obstetrics & Gynecology, Christian Medical College & Hospital, Vellore-4,Tamilnadu, India. mmuutthhuu@yahoo.com
authors.forename: Muthukumar
authors.initials: M
authors.lastname: Karthikeyan
authors.forename: Jagadeesan
authors.initials: J
authors.lastname: Arunakaran
authors.forename: Karundevi
authors.initials: K
authors.lastname: Balasubramanian
mesh_terms: D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D011388:Prolactin; D003345:Corticosterone