Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways.
We investigated the mechanism by which equine herpesvirus-1 (EHV-1) enters primary cultured equine brain microvascular endothelial cells (EBMECs) and equine dermis (E. Derm) cells. EHV-1 colocalized with caveolin in EBMECs and the infection was greatly reduced by the expression of a dominant negative form of equine caveolin-1 (ecavY14F), suggesting that EHV-1 enters EBMECs via caveolar endocytosis. EHV-1 entry into E. Derm cells was significantly reduced by ATP depletion and treatments with lysosomotropic agents. Enveloped virions were detected from E. Derm cells by infectious virus recovery assay after viral internalization, suggesting that EHV-1 enters E. Derm cells via energy- and pH-dependent endocytosis. These results suggest that EHV-1 utilizes multiple endocytic pathways in different cell types to establish productive infection.
Hasebe
Rie
R
Laboratory of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, West 9 North 18, Kita-ku, Sapporo 060-0818, Japan. r-hasebe@vetmed.hokudai.ac.jp
Sasaki
Michihito
M
Sawa
Hirofumi
H
Wada
Ryuichi
R
Umemura
Takashi
T
Kimura
Takashi
T
D000818:Animals; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004705:Endocytosis; D042783:Endothelial Cells / Q000821:virology; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000254:growth & development / Q000502:physiology*; D006734:Horse Diseases / Q000821:virology; D006736:Horses; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D019076:Transgenes; D053586:Virus Internalization
D051242:Caveolin 1; D011505:Protein-Tyrosine Kinases