title: [Analysis on the expression of the stem cells related genes in hepatocellular carcinoma cell lines].
abstract: OBJECTIVE
  To investigate the expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines of SMMC-7721, Bel-7402, HepG2, MHCC-97 and normal hepatocellular cell line of L02, and to compare the response of these cell lines to all-trans retinoic acid.
  
  
  METHODS
  RT-PCR was used to detect expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines and normal hepatocellular cell line. Real time-PCR was used to quantify the expression of the genes.
  
  
  RESULTS
  There are different levels of expression of the stem cell-related gene in hepatocellular carcinoma cell lines and control cell line (P less than 0.05). There are significant differences in HepG2 and L-02 for the response to all-trans retinoic acid (P less than 0.05).
  
  
  CONCLUSIONS
  The stem cell-related genes are differentially expressed in different hepatocellular carcinoma cell lines.
authors.affiliation: Institute of Liver Surgery, Dongfeng Hospital, Yunyang Medical College, Shiyan Hubei 442008, China.
authors.forename: Can
authors.initials: C
authors.lastname: Zou
authors.forename: Fang-jun
authors.initials: FJ
authors.lastname: Yuan
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Zhou
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Ju
authors.initials: J
authors.lastname: Wang
authors.forename: You-shun
authors.initials: YS
authors.lastname: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D000072086:Smoothened Receptor; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C491023:SMO protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D000072086:Smoothened Receptor; C435831:WNT5B protein, human; D051153:Wnt Proteins; D051176:beta Catenin; D014212:Tretinoin