Anti-inflammatory effect of parenteral fish oil lipid emulsion on human activated mononuclear leukocytes.
OBJECTIVE
To compare the effect of fish oil-based (FO) lipid emulsions (LE) for parenteral administration with standard LE and a new FO containing LE composed of four different oils on the antigen presentation and inflammatory variables.


METHODS
Phytohemagglutinin (PHA) activated human mononuclear leukocytes were cultured with different LE - Control: without LE; SO: soybean oil; SO/FO: soybean and FO (4:1); MCT/SO: medium chain triglycerides and SO (1:1); MCT/SO/FO: MCT/SO and FO (4:1) and SMOF: a new LE containing FO. Cytokine production was evaluated by ELISA, the expression of antigen-presenting and co-stimulatory surface molecules were analyzed by flow cytometry and lymphocyte proliferation was assessed by H(3)-Thymidine incorporation, after tetanus toxoid-induced activation.


RESULTS
All LE decreased the HLA-DR and increased CD28 and CD152 expression on monocytes/macrophages and lymphocytes surface (p < 0.05). SO/FO and MCT/SO/FO decreased lymphocyte proliferation (p<0.05). All LE decreased IL-2 production, but this effect was enhanced with MCT/SO/FO and SMOF (p < 0.05). MCT/SO/FO decreased IL-6 and increased IL-10, whereas SO had the opposite effect (p < 0.05).


CONCLUSIONS
FO LE inhibited lymphocyte proliferation and had an anti-inflammatory effect. These effects seem to be enhanced when FO is mixed with MCT/SO. SMOF had a neutral impact on lymphocyte proliferation and IL-6 and IL-10 production.
Manzoni Jacintho
T
T
METANUTRI/Laboratório de Fisiologia e Distúrbios Esfincterianos (LIM35), University of São Paulo Medical School, Brazil. metanutri@terr.com.br
Gotho
H
H
Gidlund
M
M
García Marques
C
C
Torrinhas
R
R
Mirtes Sales
M
M
Linetzky Waitzberg
D
D
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D007263:Infusions, Parenteral; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology*
D000893:Anti-Inflammatory Agents; D005217:Fat Emulsions, Intravenous; D005395:Fish Oils