Channel catfish (Ictalurus punctatus Rafinesque, 1818) tetraspanin membrane protein family: identification, characterization and expression analysis of CD63 cDNA.
CD63, known as lysosome associated membrane protein 3 (LAMP-3), is a member of the tetraspanin integral membrane protein family. This protein plays many important roles in immuno-physiological functions. In this communication, we report the identification, characterization, and expression analysis of the channel catfish CD63 transcript. The complete nucleic acid sequence of channel catfish CD63 cDNA was comprised of 1159 nucleotides, including an open reading frame, which appears to encode a putative peptide of 237-amino-acid residues. Like other tetraspanin proteins, the channel catfish CD63 peptide can be divided into domains, including four transmembrane domains, three intracellular domains, and one of each small and large extracellular loops. The channel catfish CD63 peptide shares 52-55% identity among fish counterparts, but only 43-46% identity among mammalian counterparts. The characteristic Cys-Cys-Gly motif and four Cys residues in the large extracellular loop were conserved. The channel catfish CD63 transcript was detected by RT-PCR in spleen, anterior kidney, liver, intestine, skin and gill. This result provides important information for further elucidating CD63 functions in channel catfish.
Yeh
Hung-Yueh
HY
United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, Auburn, AL 36832-4352, United States. hungyueh.yeh@ars.usda.gov
Klesius
Phillip H
PH
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D007059:Ictaluridae / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D060149:Tetraspanin 30; D014018:Tissue Distribution
D015703:Antigens, CD; D017931:DNA Primers; D018076:DNA, Complementary; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D060149:Tetraspanin 30