title: Mare's colostrum globules stimulate fibroblast growth in vitro: a biochemical study.
abstract: The wound repair function of mare's milk and colostrum was investigated. Mare's colostrum improved wound healing in vivo; thus fibroblast growth activation by mare's milk and colostrum was examined. As expected, colostrum was more effective than milk. To establish the biochemical nature of the bioactive molecules involved, colostrum was fractionated into whey, casein, and fat globules, and the efficacy of these fractions on fibroblast proliferation was studied. The fat globule fraction provided the strongest stimulation; its composition was studied and compared with the less-active milk fat globule fraction. The lipid pattern highlighted several differences between mare's colostrum and milk; in particular, total lipid, linoleic acid, linolenic acid, ganglioside, and glycolipid contents were higher in colostrum. A proteomic investigation revealed some differences between the protein composition of colostrum and milk fat globules. Adipophylin and lactadherin were significantly overexpressed in colostrum fat globules. The role of specific lipids on skin wound repair and that of the epidermal growth factor-like domain, embedded within the lactadherin molecule and probably released in conditions stimulating proteolysis, are discussed.
authors.affiliation: Institute of General Physiology and Biological Chemistry, University of Milan, Milan, Turin, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Zava
authors.forename: C
authors.initials: C
authors.lastname: Barello
authors.forename: A
authors.initials: A
authors.lastname: Pessione
authors.forename: L Perono
authors.initials: LP
authors.lastname: Garoffo
authors.forename: P
authors.initials: P
authors.lastname: Fattori
authors.forename: G
authors.initials: G
authors.lastname: Montorfano
authors.forename: A
authors.initials: A
authors.lastname: Conti
authors.forename: C
authors.initials: C
authors.lastname: Giunta
authors.forename: E
authors.initials: E
authors.lastname: Pessione
authors.forename: B
authors.initials: B
authors.lastname: Berra
authors.forename: M G
authors.initials: MG
authors.lastname: Giuffrida
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002364:Caseins / Q000302:isolation & purification; D049109:Cell Proliferation / Q000187:drug effects; D002784:Cholesterol / Q000032:analysis; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005732:Gangliosides / Q000032:analysis; D006017:Glycolipids / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology; D006736:Horses; D006801:Humans; D066292:Lipid Droplets; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D011247:Pregnancy; D040901:Proteomics; D012867:Skin / Q000187:drug effects; D014280:Triglycerides / Q000032:analysis; D000067816:Whey Proteins; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002364:Caseins; D005732:Gangliosides; D006017:Glycolipids; D006023:Glycoproteins; D008055:Lipids; D008894:Milk Proteins; D014280:Triglycerides; D000067816:Whey Proteins; C110367:milk fat globule; D002784:Cholesterol