title: Probiotic potential of Staphylococcus hominis MBBL 2-9 as anti-Staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman.
abstract: OBJECTIVE
  To isolate and characterize an antagonist for use as probiotic agent in the biocontrol of Staphylococcus aureus.
  
  
  RESULTS
  Bacteria that exhibited antimicrobial activity against Gram-positive bacteria including Staph. aureus were isolated from 12 healthy women, with Staphylococcus hominis MBBL 2-9 showing the strongest activity. The bacteriocin produced by Staph. hominis MBBL 2-9 was purified by 60% ammonium sulfate saturation, ultrafiltration, HLB cartridge and reverse-phase HPLC. The molecular weight was estimated as 2038.2 Da by MALDI-TOF mass spectrometry. The antagonist survived up to 2 h in artificial gastric juice (pH 2.5) and grew in the presence of 1% porcine bile extract. In addition, Staph. hominis MBBL 2-9 adhered effectively to HT-29 epithelial cell line.
  
  
  CONCLUSIONS
  Staphylococcus hominis MBBL 2-9 exhibited desirable probiotic traits such as acid tolerance, bile resistance and adherence to epithelial cell line. The bacterium also produced a bacteriocin with unique molecular weight and high antimicrobial activity similar to traditional antibiotics.
  
  
  CONCLUSIONS
  This study is the first report of a bacteriocin-producing Staph. hominis MBBL 2-9 that has potential for use as a probiotic agent against Staph. aureus.
authors.affiliation:  Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, Korea.
authors.forename: C
authors.initials: C
authors.lastname: Sung
authors.affiliation:  School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea.| Institute of Bioengineering, Seoul National University, Seoul, Korea.
authors.forename: B-G
authors.initials: BG
authors.lastname: Kim
authors.affiliation:  Division of Food Science, Korea University, Anam-dong, Sungbuk-ku, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.affiliation:  School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea.
authors.forename: H-S
authors.initials: HS
authors.lastname: Joo
authors.affiliation:  School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea.
authors.forename: P I
authors.initials: PI
authors.lastname: Kim
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001422:Bacterial Adhesion; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D019936:Probiotics; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D041181:Staphylococcus hominis / Q000378:metabolism / Q000502:physiology*; D014621:Vagina / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins