High-speed cell sorting of infectious trophic and cystic forms of Pneumocystis carinii.
The separation of Pneumocystis carinii life-cycle stages while preserving infectivity is a hitherto unresolved challenge. We describe an original, reproducible, and efficient method for separating trophic from cystic forms of P. carinii using a high-speed cell sorter. The large amounts of highly purified (99.6+/-0.3%) infectious trophic and cystic forms can now be used to elucidate the poorly understood P. carinii life cycle.
Martinez
Anna
A
Department of Parasitology, Lille Nord de France University, France.
Aliouat
El Moukhtar
el M
Pottier
Muriel
M
Gantois
Nausicâa
N
Pinçon
Claire
C
Standaert-Vitse
Annie
A
Dei-Cas
Eduardo
E
Aliouat-Denis
Cécile-Marie
CM
D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D045363:Pneumocystis carinii / Q000145:classification* / Q000302:isolation & purification*