Proteomics-based approach for identification and purification of human phosphate binding apolipoprotein from amniotic fluid.
Human amniotic fluid is of both maternal and fetal origin; it protects the fetus and provides the environment for growth and development of the fetus. We used a proteomics-based approach for targeting and purifying human phosphate binding protein, a member of the DING family of proteins from amniotic fluid, using Blue Sepharose CL-6B, DEAE-Sephacel and gel filtration chromatography. The protein had earlier been reported to be serendipitously purified along with PON1 (paraoxonase 1). It was identified using electro-spray-ionization-time-of-flight mass spectrometry and was found to be human phosphate binding protein. Human phosphate binding proteins have been reported to play a role as phosphate scavengers and may have a protective function against phosphate-related disorders, such as atherosclerosis, diabetes and kidney stones.
Alam
M
M
Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
Mahajan
M
M
Raziuddin
M
M
Singh
T P
TP
Yadav
S
S
D000595:Amino Acid Sequence; D000653:Amniotic Fluid / Q000737:chemistry*; D001053:Apolipoproteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D028044:Phosphate-Binding Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D012203:Rh Isoimmunization; D013056:Spectrophotometry, Ultraviolet
D001053:Apolipoproteins; D010455:Peptides; D028044:Phosphate-Binding Proteins