Selective sample pretreatment by molecularly imprinted polymer for the determination of LSD in biological fluids.
For the first time, a molecularly imprinted polymer (MIP) was synthesized by a noncovalent imprinting approach for the selective extraction of an illicit drug, LSD, from hair and urine samples. For the synthesis of MIP, an analog of LSD, was taken as a dummy template, methacrylic acid as a functional monomer, and ACN as a porogen solvent. The MIP was used for offline extraction before HPLC-MS analysis. By studying the interactions taking place between the LSD and the MIP, a selective procedure was established in organic media and applied to hair samples. By this way, 0.1 ng/mg of LSD was successfully detected in hair with 82% of extraction recovery. A low retention was also obtained on the control polymer (only 9%). This procedure was then modified to obtain a selective extraction in aqueous media for the determination of LSD in urine samples. The comparison with a conventional C18 clearly demonstrated the selectivity brought by the MIP to the determination of LSD in urine. LSD was easily detected in urine at only 0.5 ng/mL with 83% of extraction recovery on the MIP and 11% on the NIP. An LOQ of 0.2 pg/mL was estimated in urine samples.
Chapuis-Hugon
Florence
F
Department of Environmental and Analytical Chemistry UMR PECSA 7195, Ecole Supérieure de Physique et de Chimie Industrielles (ESPCI), Paris, France. florence.hugon@espci.fr
Cruz-Vera
Marta
M
Savane
Ramatoulaye
R
Ali
Wassim Hadj
WH
Valcarcel
Miguel
M
Deveaux
Marc
M
Pichon
Valerie
V
D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006197:Hair / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008238:Lysergic Acid Diethylamide / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
D011108:Polymers; D012997:Solvents; D008238:Lysergic Acid Diethylamide