A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae.
We report a multiplatform real-time polymerase chain reaction methodology based on genes encoding for the regulatory toxR activator and enterotoxin A protein to determine enterotoxigenic Vibrio cholerae types from other vibrios. This assay, which was successfully validated on a collection of 87 bacterial strains, including 63 representatives of V. cholerae and 8 noncholera vibrios provides a rapid tool for detection and identification of cholera.
Koskela
Katja A
KA
Centres for Military Medicine and for Biological Threat Preparedness, FI-00290 Helsinki, Finland.
Matero
Pirjo
P
Blatny
Janet M
JM
Fykse
Else M
EM
Olsen
Jaran Strand
JS
Nuotio
Lasse O
LO
Nikkari
Simo
S
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D002771:Cholera / Q000175:diagnosis / Q000382:microbiology; D004268:DNA-Binding Proteins / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics; D004783:Environmental Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004268:DNA-Binding Proteins; D004768:Enterotoxins; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae