title: Morphological and molecular characterization of Strongyloides ophidiae (Nematoda, Strongyloididae).
abstract: The aim of the present study is to report morphological data from parasitic female, rhabditoid and filarioid larvae, free-living female worms and eggs of Strongyloides ophidiae (Nematoda, Strongyloididae). In addition, a molecular DNA analysis was carried out using a pool of eight S. ophidiae parasitic females. Samples were obtained from the small intestine of Oxyrhopus guibei (Serpentes, Colubridae) collected in the municipality of Lençóis Paulista, State of São Paulo, Brazil. DNA amplification by polymerase chain reaction (PCR) resulted in a 350 bp band for samples containing S. ophidiae and Strongyloides venezuelensis DNA. Strongyloides ophidiae nucleotide sequence analysis showed 98% similarity with Strongyloides procyonis and 97% with Strongyloides cebus, Strongyloides stercoralis, Strongyloides fuelleborni and Strongyloides sp. from snakes.
authors.affiliation: Departamento de Medicina Veterinária, Universidade Federal do Piauí, Campus Cinobelina Elvas, Bom Jesus, Piauí, Brazil.
authors.forename: K R
authors.initials: KR
authors.lastname: dos Santos
authors.forename: B C
authors.initials: BC
authors.lastname: Carlos
authors.forename: K S
authors.initials: KS
authors.lastname: Paduan
authors.forename: S M
authors.initials: SM
authors.lastname: Kadri
authors.forename: T H
authors.initials: TH
authors.lastname: Barrella
authors.forename: M R V
authors.initials: MR
authors.lastname: Amarante
authors.forename: P E M
authors.initials: PE
authors.lastname: Ribolla
authors.forename: R J
authors.initials: RJ
authors.lastname: da Silva
mesh_terms: D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D017812:Colubridae / Q000469:parasitology; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005260:Female; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013321:Strongyloides / Q000033:anatomy & histology* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S