title: Growth and survival differences of Vibrio vulnificus and Vibrio parahaemolyticus strains during cold storage.
abstract: Vibrio vulnificus and Vibrio parahaemolyticus are the most common Vibrio species associated with seafood illness in the United States. Our study was conducted to determine if strain-to-strain differences exist in the growth and survival of 8 different V. vulnificus and V. parahaemolyticus strains at low temperatures. By day 10, V. vulnificus strain 515-4C2 had significantly higher counts (P < 0.05) (1.97 log CFU/g) compared with strains 3315, 1007, 29306 at 5 degrees C, which reached nondetectable levels. At 8 degrees C, strain 515-4C2 had significantly higher counts (P < 0.05) (2.23 log CFU/mL) compared with 1007, 33815, 541(O) 49C, which reached nondetectable levels. At 10 degrees C, only V. vulnificus strain 33815 reached nondetectable levels. At 5 degrees C, V. parahaemolyticus strain 541(O) 57C had the highest counts (5.28 log CFU/g) by day 10 while strain 33847 had significantly lower counts (3.46 log CFU/g). After 10 d at 8 degrees C, V. parahaemolyticus strain M350A had the highest counts (7.97 log CFU/mL) while strain 541(O) 57C had the lowest counts (4.80 log CFU/mL). At 10 degrees C, V. parahaemolyticus strain NY477 had significantly higher counts (P < 0.05) with 8.31 log CFU/mL compared with strain 33847, which had the lowest counts (6.77 log CFU/mL). Our research has shown that various V. vulnificus and V. parahaemolyticus strains vary in their ability to survive and grow at refrigeration temperatures.
authors.affiliation: Dept. of Food Science, Louisiana State Univ. Agricultural Center, Baton Rouge, LA 70803, USA.
authors.forename: V E
authors.initials: VE
authors.lastname: Burnham
authors.forename: M E
authors.initials: ME
authors.lastname: Janes
authors.forename: L A
authors.initials: LA
authors.lastname: Jakus
authors.forename: J
authors.initials: J
authors.lastname: Supan
authors.forename: A
authors.initials: A
authors.lastname: DePaola
authors.forename: J
authors.initials: J
authors.lastname: Bell
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015169:Colony Count, Microbial; D005516:Food Microbiology; D016018:Least-Squares Analysis; D010125:Ostreidae / Q000382:microbiology; D012034:Refrigeration; D017747:Seafood / Q000382:microbiology; D013045:Species Specificity; D013997:Time Factors; D014736:Vibrio parahaemolyticus / Q000254:growth & development* / Q000302:isolation & purification; D041261:Vibrio vulnificus / Q000254:growth & development* / Q000302:isolation & purification