title: An interference study of the copper-induced plasma oxidizability test.
abstract: OBJECTIVE
  The aim of this study is to investigate the effect of cristaloid cardioplegic fluid (CCF) and its contents on the susceptibility of plasma to copper-catalyzed lipid peroxidation test.
  
  
  METHODS
  The plasma pool was divided into eight groups. Equal volumes of CCF or one of its contents were added to each group of the plasma pool. The accumulation of conjugated diene (CD) by copper-induced oxidation was monitored for a period of 5 h. Thiobarbituric acid-reactive substances (TBARS) formed during the incubation of plasma with copper was also measured.
  
  
  RESULTS
  It was found that, the production of CD and TBARS were inhibited and the lag time had increased, when the plasma was mixed with CCF or its contents.
  
  
  CONCLUSIONS
  As a result, we conclude that that the susceptibility of plasma to copper-induced lipid peroxidation is interfered by CCF. The chloride ions, which major content of CCF, may play an important role on this effect.
authors.affiliation: Department of Biochemistry, University of Trakya, Faculty of Medicine, Edirne, Turkey. drseskiocak@yahoo.co.uk
authors.forename: Sevgi
authors.initials: S
authors.lastname: Eskiocak
authors.forename: Turan
authors.initials: T
authors.lastname: Ege
authors.forename: Suleyman Bedir
authors.initials: SB
authors.lastname: Yapar
authors.forename: Sabriye
authors.initials: S
authors.lastname: Kaya
authors.forename: Gulben
authors.initials: G
authors.lastname: Sayilan
mesh_terms: D000328:Adult; D003300:Copper / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper