Transillumination fluorescence imaging in mice using biocompatible upconverting nanoparticles.
We report on a systematic study of upconverting fluorescence signal generation within turbid phantoms and real tissues. An accurate three-point Green's function, describing the forward model of photon propagation, is established and experimentally validated. We further demonstrate, for the first time to our knowledge, autofluorescence-free transillumination imaging of mice that have received biocompatible upconverting nanoparticles. The method holds great promise for artifact-free whole-body visualization of optical molecular probes.
Vinegoni
Claudio
C
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA. cvinegoni@mgh.harvard.edu
Razansky
Daniel
D
Hilderbrand
Scott A
SA
Shao
Fangwei
F
Ntziachristos
Vasilis
V
Weissleder
Ralph
R
D000818:Animals; D016477:Artifacts; D001672:Biocompatible Materials / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics; D014172:Transillumination
D001672:Biocompatible Materials; C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes