title: A (238)Pu irradiator for exposure of cultured cells with alpha-radiation: construction, calibration and dosimetry.
abstract: An alpha-particle irradiator that can facilitate investigations of alpha-radiation effects on human cells in radiation protection, carcinogenesis and radioimmunotherapy was constructed. The irradiator was based on a 1.3 GBq (238)Pu source, housed in a stainless steel tube flushed with helium. Radiation provided by (238)Pu consists mainly of alpha-particles with energy of 5.5 MeV. The alpha-particle fluence and energy spectra were measured with a silicon semiconductor detector. Monte Carlo simulations were used to estimate the mean number of alpha-particles and the mean absorbed alpha-particle dose to cells for various irradiation times and distances between cells and source. There was a linear dependence between exposure time and alpha-particle fluence for exposure times above 1s. The alpha-particle activity concentration varied with a factor 2.7 over the source area, while the variation in energy peak position was <4%. At the cell nucleus position and with a distance of 45 mm between the source and the mylar dish surface, the alpha-fluence was 4.6 x 10(4)counts/(mm(2)s), the average incident alpha-particle energy was 2.5 MeV and the average linear energy transfer was 167 keV/microm. The average dose rate to the cells, with 5 microm diameter nucleus, was 1.2 Gy/s. The (238)Pu alpha-particle irradiator is feasible for irradiation of cells and it can be used for studies of both direct effects and bystander effects of alpha-radiation.
authors.affiliation: Department of Radiation Biology, The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: Nikolai
authors.initials: N
authors.lastname: Tisnek
authors.forename: Erta
authors.initials: E
authors.lastname: Kalanxhi
authors.forename: Camilla Walle
authors.initials: CW
authors.lastname: Serkland
authors.forename: Jørn
authors.initials: J
authors.lastname: Iversen
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Belyakov
authors.forename: Jostein
authors.initials: J
authors.lastname: Dahle
mesh_terms: D000512:Alpha Particles; D018929:Cell Culture Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D011005:Plutonium / Q000737:chemistry*; D011829:Radiation Dosage; D055618:Radiation Equipment and Supplies; D011874:Radiometry / Q000295:instrumentation*
chemical_list: D011005:Plutonium