A possible novel Francisella genomic species isolated from blood and urine of a patient with severe illness.
Two identical isolates were recovered in pure culture from the blood and urine of a patient suffering from severe septicaemia associated with obstructive pyelonephritis secondary to lithotripsy. Preliminary phenotypic and genotypic characterizations based on serological, biochemical and sequence analyses following PCR amplification of selected gene regions indicate that this organism represents a potential new Francisella genomic species.
Escudero
R
R
Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. rescude@isciii.es
Elía
M
M
Sáez-Nieto
J A
JA
Menéndez
V
V
Toledo
A
A
Royo
G
G
Rodríguez-Vargas
M
M
Whipp
M J
MJ
Gil
H
H
Jado
I
I
Anda
P
P
D000328:Adult; D016470:Bacteremia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005603:Francisella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D020459:Genes, rRNA; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D008096:Lithotripsy / Q000009:adverse effects; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011704:Pyelonephritis / Q000209:etiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D014556:Urine / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S