title: A novel magnetic crystal-lipid nanostructure for magnetically guided in vivo gene delivery.
abstract: Cancer gene therapy requires a safe and effective gene delivery system. Polymer- and lipid-coated magnetic nanocrystals have been used to deliver silencing RNA, but synthesizing these magnetic vectors is difficult. Here, we show that a new nanoparticle formulation can be magnetically guided to deliver and silence genes in cells and tumours in mice. This formulation, termed LipoMag, consists of an oleic acid-coated magnetic nanocrystal core and a cationic lipid shell. When compared with the commercially available PolyMag formulation, LipoMag displayed more efficient gene silencing in 9 of 13 cell lines, and better anti-tumour effects when systemically administered to mice bearing gastric tumours. By delivering an optimized sequence of a silencing RNA that targets the epidermal growth factor receptor of tumour vessels, the intended therapeutic benefit was achieved with no evident adverse immune reaction or untoward side effects.
authors.affiliation: Institute of Clinical Medicine and Research, The Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba, Japan. cancer_gene_therapy@ybb.ne.jp
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Namiki
authors.forename: Tamami
authors.initials: T
authors.lastname: Namiki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ishii
authors.forename: Akihito
authors.initials: A
authors.lastname: Tsubota
authors.forename: Shigeo
authors.initials: S
authors.lastname: Koido
authors.forename: Kouichi
authors.initials: K
authors.lastname: Nariai
authors.forename: Makoto
authors.initials: M
authors.lastname: Mitsunaga
authors.forename: Satoru
authors.initials: S
authors.lastname: Yanagisawa
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kashiwagi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Mabashi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yumoto
authors.forename: Sadayori
authors.initials: S
authors.lastname: Hoshina
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Fujise
authors.forename: Norio
authors.initials: N
authors.lastname: Tada
mesh_terms: D003460:Crystallization / Q000379:methods; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry*; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D008280:Magnetics; D050997:Nanomedicine / Q000379:methods*; D049329:Nanostructures / Q000627:therapeutic use*; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D008055:Lipids; D004247:DNA