Caspase-dependent apoptosis of ON bipolar cells in the goldfish marginal retina.
Progenitor cells proliferate at the retinal margin of adult goldfish, so that the developmental stages of maturing retinal cells are arranged in temporal sequence along the periphero-central axis. We studied the caspase-dependent apoptosis of ON bipolar cells, which were labeled by an antibody to protein kinase C (PKC). In both primary and secondary growth zones of the marginal retina, PKC+ cell somata were of two distinct sizes, large and small. A caspase inhibitor (zVAD-fmk), injected into the vitreous, increased the number of both large and small PKC+ somata (times approximately 2 and 1.1, respectively) in both growth zones. These data show that about one-third of immature ON bipolar cells were removed by caspase-dependent apoptosis before being incorporated into the mature retina.
Mizuno
Taka-aki
TA
Toho University, Miyama, Funabashi, Chiba, Japan.
Ohtsuka
Teruya
T
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D020169:Caspases / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006054:Goldfish; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D051245:Retinal Bipolar Cells / Q000166:cytology / Q000187:drug effects*; D012162:Retinal Degeneration / Q000201:enzymology / Q000503:physiopathology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D000590:Amino Acid Chloromethyl Ketones; D004791:Enzyme Inhibitors; C484771:benzyloxycarbonyl-valyl-aspartic acid fluoromethyl ketone; D011493:Protein Kinase C; D020169:Caspases