In vivo infection of IgG-containing cells by Jembrana disease virus during acute infection.
Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cells in which the viral capsid protein could be detected. Viral antigen was present in pleomorphic centroblast-like cells which were identified as IgG-containing cells, including plasma cells, in lymphoid tissues. There was no evidence of infection of CD3(+) T-cells or MAC387(+) monocytes in tissues but large vacuolated cells with a macrophage-like morphology in the lung were found to contain viral antigen although they could not be shown conclusively to be infected. The tropism of JDV for mature IgG-containing cells may be relevant to understanding the pathogenesis of Jembrana disease, the delayed antibody responses and the genetic composition of this atypical lentivirus.
Desport
Moira
M
School of Veterinary and Biomedical Science, Murdoch University, Murdoch WA 6150, Australia. mdesport@murdoch.edu.au
Tenaya
I W Masa
IW
McLachlan
Alexander
A
McNab
Tegan J
TJ
Rachmat
Judhi
J
Hartaningsih
Nining
N
Wilcox
Graham E
GE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000821:virology; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D016180:Lentivirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017960:Lentiviruses, Bovine / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000821:virology; D008221:Lymphoid Tissue / Q000276:immunology / Q000821:virology; D008264:Macrophages / Q000276:immunology / Q000821:virology; D009000:Monocytes / Q000276:immunology / Q000821:virology; D010950:Plasma Cells / Q000276:immunology / Q000821:virology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G