[Effect of Astragalus polysaccharides on the phenotype and functions of human dendritic cells in vitro].
OBJECTIVE
To assess the effect of astragalus polysaccharides (APS) in inducing phenotypic and functional changes of human dendritic cells (DCs) in vitro.


METHODS
Human dendritic cells were induced from the peripheral blood monocytes in vitro by the application of tumor necrosis factor-alpha (TNF-alpha), interleukin-4 (IL-4) and GM-CSF, and cultured in the presence of APS at different concentrations (50, 100, and 200 mg/L). The morphological changes of the DCs were identified by optical microscope or scanning electron microscope. The phenotypic alterations of the cells were analyzed by flow cytometry.


RESULTS
The DCs cultured for 24 h in the presence of LPS and APS at 50 and 100 mg/L showed suspended growth in the culture medium and underwent morphological changes from spherical cells to irregular cells, with rough cell surface and cell processes of different morphologies. APS-treated DCs had the most typical dendritic structures and highly expressed the phenotypic markers of DCs (CD86 and HLA-DR), but with down-regulated CD14 expression as shown by flow cytometry.


CONCLUSIONS
Both APS and the cytokines can induce the maturation of DCs derived from peripheral blood monocytes.
Chen
Chao-Jun
CJ
Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. ccjbs@126.com
Li
Zhi-Liang
ZL
Fu
Qiang
Q
Liu
Yi
Y
Lei
Xiao
X
Wu
Hong-Chao
HC
Liu
Ying-Feng
YF
D027885:Astragalus propinquus / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology; D010641:Phenotype; D011134:Polysaccharides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor