title: Feasibility of chemohyperthermia with docetaxel-embedded magnetoliposomes as minimally invasive local treatment for cancer.
abstract: Hyperthermia is a minimally invasive approach to cancer treatment, but it is difficult to heat only the tumor without damaging surrounding tissue. To solve this problem, we studied the effectiveness of chemohyperthermia with docetaxel-embedded magnetoliposomes (DMLs) and an applied alternating current (AC) magnetic field. Human MKN45 gastric cancer cells were implanted in the hind limb of Balb-c/nu/nu mice. Various concentrations of docetaxel-embedded DMLs were injected into the tumors and exposed to an AC magnetic field (n = 6, each). For comparison with hyperthermia alone, magnetite-loaded liposome (ML)-injected tumors were exposed to an AC magnetic field. Furthermore, the results of DML without AC treatment and docetaxel diluted into PBS with AC treatment were also compared (n = 10, each). Tumor surface temperature was maintained between 42 and 43 degrees C. Tumor volume was reduced in the DML group with a docetaxel concentration > 56.8 microg/ml, while a docetaxel concentration > 568.5 microg/ml was required for tumor reduction without hyperthermia. Statistically significant differences in tumor volume and survival rate were observed between the DML group exposed to the magnetic field and the other groups. The tumor disappeared in 3 mice in the DML group exposed to the magnetic field; 2 mice survived over 6 months after treatment, whereas all mice of the other groups died by 15 weeks. Histologically, hyperthermia with DML damaged tumor cells and DML diffused homogeneously. To the best of our knowledge, this is the first report to show that hyperthermia using chemotherapeutic agent-embedded magnetoliposomes has an anticancer effect.
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Motohira
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: AdMeTech Co., Ltd., Matsuyama, Ehime, Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Sato
authors.affiliation: Departments of Condensed Matter and Plasma Physics, Ehime University Graduate School of Science and Engineering, Matsuyama, Ehime, Japan.
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Maehara
authors.affiliation: Departments of Applied Chemical Physics, Ehime University Graduate School of Science and Engineering, Matsuyama, Ehime, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Aono
authors.affiliation: Departments of Applied Chemical Physics, Ehime University Graduate School of Science and Engineering, Matsuyama, Ehime, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Naohara
authors.affiliation: Departments of Applied Chemical Physics, Ehime University Graduate School of Science and Engineering, Matsuyama, Ehime, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Hirazawa
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Horiuchi
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Shungo
authors.initials: S
authors.lastname: Yukumi
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Sato
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nakagawa
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sugishita
authors.affiliation: Department of Organ Regenerative Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Kanji
authors.initials: K
authors.lastname: Kawachi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D000077143:Docetaxel; D055590:Electromagnetic Phenomena; D005240:Feasibility Studies; D005260:Female; D052203:Ferrosoferric Oxide / Q000008:administration & dosage*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D008081:Liposomes; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D043823:Taxoids / Q000008:administration & dosage*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D043823:Taxoids; D000077143:Docetaxel; D052203:Ferrosoferric Oxide