Psychrobacter aestuarii sp. nov., isolated from a tidal flat sediment.
A Gram-negative-staining, non-motile, non-spore-forming and strictly aerobic bacterial strain, SC35(T), was isolated from tidal flat sediment collected from the South Sea, Korea, and subjected to a taxonomic study using a polyphasic approach. The organism grew optimally at 20-30 degrees C and with 1-2 % (w/v) NaCl. Strain SC35(T) contained ubiquinone-8 as the predominant respiratory lipoquinone and C(18 : 1)omega9c as the major fatty acid. The DNA G+C content was 48.5 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain SC35(T) formed a lineage within the genus Psychrobacter (94.3-96.5 % sequence similarity), forming a distinct branch in a clade also containing Psychrobacter pacificensis NIBH P2K6(T) and Psychrobacter celer SW-238(T). On the basis of phenotypic and phylogenetic data, strain SC35(T) (=KCTC 22503(T)=JCM 16343(T)) was placed in the genus Psychrobacter as the type strain of a novel species, for which the name Psychrobacter aestuarii sp. nov. is proposed.
Baik
Keun Sik
KS
Department of Biological Science, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Park
Seong Chan
SC
Department of Biological Science, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Lim
Chae Hong
CH
Department of Biological Science, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Lee
Kang Hyun
KH
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong, Daejeon 305-333, Republic of Korea.
Jeon
Doo Young
DY
Microbiology Division, Jeollanam-do Institute of Health and Environment, Gwangju, 502-810, Republic of Korea.
Kim
Choong Mo
CM
Microbiology Division, Jeollanam-do Institute of Health and Environment, Gwangju, 502-810, Republic of Korea.
Seong
Chi Nam
CN
Department of Biological Science, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
D001483:Base Sequence; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D044096:Psychrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S