title: [Formation of biofilms by fecal strains of enterobacteria and yeast fungi from Candida genus].
abstract: OBJECTIVE
  To assess the ability of bacteria and Candida genus' yeast fungi from human fecal microflora to form biofilms.
  
  
  METHODS
  Thirty-one strains of enterobacteria and 45 strains of yeast fungi from Candida genus isolated from feces of patients during test on intestinal dysbiosis were used. In order to study the ability of microorganisms to form biofilms, level of binding of crystal violet by the formers in sterile 96-well polystyrene plates was studied.
  
  
  RESULTS
  Ability to form biofilms was noted in 87.1% of enterobacteria strains. This characteristic was observed in lactose-positive and lactose-negative Escherichia coli and bacteria belonging to Klebsiella, Citrobacter, Serratia, Morganella, Providencia genuses. The highest mean value of the characteristic was noted in strains belonging to Enterobacter cloacae species (6.52+/-2.01). Overwhelming majority of fungal strains belonging to Candida genus formed static biofilms in wells of polystyrene plates. Mean value of the characteristic in group of Candida spp. (not albicans) was significantly higher compared with strains from C. albicans species (2.75+/-0.53 vs. 1.38+/-0.11, p<0.05). The highest value of the mentioned characteristic (6.75+/-1.65) was observed in yeast fungi belonging to C. krusei species.
  
  
  CONCLUSIONS
  Obtained data widen our knowledge about armamentarium of biological characteristics of opportunistic microorganisms (bacteria and fungi) promoting their prolonged persistence in intestinal biotope.
authors.forename: A V
authors.initials: AV
authors.lastname: Valyshev
authors.forename: I V
authors.initials: IV
authors.lastname: Valysheva
authors.forename: I V
authors.initials: IV
authors.lastname: Geĭde
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology*; D004755:Enterobacteriaceae / Q000502:physiology*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007410:Intestinal Diseases / Q000382:microbiology*