Overexpression of Newcastle disease virus (NDV)  V protein enhances NDV production kinetics  in chicken embryo fibroblasts.
Newcastle disease virus (NDV) is not only one of the most economically important pathogen of poultry but also has a potential as anticancer virotherapy. The role of NDV V protein in virus-production kinetics was investigated using DF-1 cell-based production system. The presence of an anti-interferon (IFN)-alpha antibody resulted in enhanced NDV production kinetics in a dose-dependent manner by blocking binding of NDV-induced IFN to its receptor. To prepare DF-1 cell whose cellular IFN signaling is blocked efficiently, stable cell lines expressing either lentogenic or velogenic NDV V protein known as an IFN antagonist were established. The overexpression of NDV V protein enhanced NDV production kinetics and expedited the rate of NDV production, while it had no effect on Japanese encephalitis virus production. NDV V protein functions as an IFN antagonist by inhibiting the increase in type I IFNs by NDV infection. The IFN signals in cells expressing NDV V protein were weakened by decreased activation or expression of the dsRNA-activated enzymes. These IFN antagonist activities enhance rapid virus replication and spread in the early phase of viral infection and will be useful in improving the production of viral vaccine strains.
Jang
Juno
J
School of Life Sciences and Biotechnology, Korea University, Seongbuk-Gu, Seoul 136-701, Republic of Korea.
Hong
Sung-Hwan
SH
Choi
Dongwon
D
Choi
Kang-Seuk
KS
Kang
Seongman
S
Kim
Ik-Hwan
IH
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004664:Encephalitis Virus, Japanese / Q000502:physiology; D005347:Fibroblasts / Q000276:immunology / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D007113:Immunity, Innate; D016898:Interferon-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D053633:Receptor, Interferon alpha-beta / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D016898:Interferon-alpha; D014764:Viral Proteins; D053633:Receptor, Interferon alpha-beta