Effects of electrokinetic treatment of a heavy metal contaminated soil on soil enzyme activities.
There is a growing concern on the potential application of a direct current (DC) electric field to soil for removing contaminants, but little is known about its impact on soil enzyme activities. This study investigated the change of enzyme activities of a heavy metal contaminated soil before and after electrokinetic (EK) treatments at lab-scale and the mechanisms of EK treatment to affect soil enzyme activities were explored. After treatments with 1-3 V cm(-1) of voltage gradient for 420 h, soil pH, electrical conductivity (EC), soil organic carbon, dissolved organic carbon (DOC), soil heavy metal concentration and enzyme activities were analyzed. The results showed that the average removal efficiencies of soil copper were about 65% and 83% without and with pH control of catholyte, respectively, and all the removal efficiencies of cadmium were above 90%. The soil invertase and catalase activities increased and the highest invertase activity was as 170 times as the initial one. The activities of soil urease and acidic phosphatase were lower than the initial ones. Bivariate correlation analyses indicated that the soil invertase and acidic phosphatase activities were significantly correlated with soil pH, EC, and DOC at P<0.05, but the soil urease activities had no correlation with the soil properties. On the other hand, the effects of DC electric current on solution invertase and catalase enzyme protein activities indicated that it had negative effect on solution catalase activity and little effect on solution invertase activity. From the change of invertase and catalase activities in soil and solution, the conclusion can be drawn that the dominant effect mechanism is the change of soil properties by EK treatments.
Cang
Long
L
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Zhou
Dong-Mei
DM
Wang
Quan-Ying
QY
Wu
Dan-Ya
DY
D000135:Acid Phosphatase; D002104:Cadmium; D002244:Carbon; D002374:Catalase; D003300:Copper; D055664:Electrochemical Techniques; D004798:Enzymes / Q000378:metabolism*; D019216:Metals, Heavy / Q000737:chemistry*; D012987:Soil; D012989:Soil Pollutants / Q000737:chemistry*; D014510:Urease; D043324:beta-Fructofuranosidase
D004798:Enzymes; D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; D002244:Carbon; D003300:Copper; D002374:Catalase; D000135:Acid Phosphatase; D043324:beta-Fructofuranosidase; D014510:Urease