title: Adsorption of cellulase on cellulolytic enzyme lignin from lodgepole pine.
abstract: Enzymatic hydrolysis of lignocellulosic materials is significantly affected by cellulase adsorption onto the lignocellulosic substrates and lignin. The presence of lignin plays an important role in lignocellulosic hydrolysis and enzyme recycling. Three cellulase preparations (Celluclast, Spezyme CP, and MSUBC) were evaluated to determine their adsorption onto cellulolytic enzyme lignin (CEL) from steam-exploded Lodgepole pine (SELP) and ethanol (organosolv)-pretreated Lodgepole pine (EPLP). The adsorption affinity of cellulase (Celluclast) onto isolated lignin (CEL-EPLP and CEL-SELP) was slightly higher than that from corresponding EPLP and SELP substrates on the basis of the Langmuir constants. Effects of temperature, ionic strength, and surfactant on cellulase adsorption onto isolated lignin were also explored in this study. Thermodynamic analysis of enzyme adsorption onto isolated lignin (Gibbs free energy change DeltaG(0) approximately -30 kJ/mol) indicated this adsorption was a spontaneous process. The addition of surfactant (0.2% w/v) could reduce the adsorption of cellulase onto CEL-SELP by 60%. Two types of adsorption isotherm were compared for cellulase adsorption onto isolated lignin. A Langmuir adsorption isotherm showed better fit for the experimental data than a Freundlich adsorption isotherm.
authors.affiliation: School of Forestry and Wildlife Sciences, Auburn University, Auburn, Alabama 36849, USA. mtu@auburn.edu
authors.forename: Maobing
authors.initials: M
authors.lastname: Tu
authors.forename: Xuejun
authors.initials: X
authors.lastname: Pan
authors.forename: Jack N
authors.initials: JN
authors.lastname: Saddler
mesh_terms: D000327:Adsorption; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D009994:Osmolar Concentration; D028223:Pinus / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
chemical_list: C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D002480:Cellulase