[Effect of liquorice decoction on rat intestinal P-glycoprotein].
OBJECTIVE
To investigate the effect of liquorice in functional modulation of intestinal P-glycoprotein (P-gp) in rats.


METHODS
An in vitro diffusion chamber system (Ussing chamber) was used to examine the direct effect of liquorice decoction on rhodamine 123 (a subtrate of P-gp) transport and evaluate the permeability of rhodamine 123 or fluorescein sodium through rat jejunum membranes after oral administration of liquorice decoction.


RESULTS
Direct application of liquorice decoction did not obviously affect rhodamine 123 transport across the intestinal mucosa. Oral administration of liquorice decoction (10 g/kg, twice daily for a week) significantly increased the absorption of rhodamine 123 and also enhanced rhodamine 123 secretion across the jejunum mucosa. Liquorice had no obvious effect on the transport of CF across the jejunum mucosa.


CONCLUSIONS
Liquorice may slightly inhibit P-gp function in the intestinal mucosa to increase the intestinal absorption of rhodamine 123.
Yao
Hong-wei
HW
Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Fu
Xiao-you
XY
Xie
Qing-dong
QD
Huang
Bei-bei
BB
Sun
Ya-bin
YB
Li
Guo-feng
GF
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D006035:Glycyrrhiza; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020112:Rhodamine 123 / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D010936:Plant Extracts; C069074:liquorice decocta; D020112:Rhodamine 123