title: CGG repeat sizing in the FMR1 gene in Indian women with premature ovarian failure.
abstract: The CGG repeat stretch in the FMR1 gene is polymorphic, ranging from 5 to 50 repeats in the normal population. Expansion of the repeats to the premutation range (50-200) has been associated with premature ovarian failure (POF). This case-control study was conducted to enumerate CGG repeats in the FMR1 gene in 80 Indian women with non-familial, non-syndromic POF, and 70 controls from the same ethnicity. A possible association between CGG repeats and endocrine profile of these cases was investigated. All patients and controls had CGG repeats in the normal polymorphic range. Serum FSH concentrations were significantly raised in both POF cases and controls having CGG repeats in the 31-40 repeats range (P < 0.0001). POF cases and controls had FSH concentrations of 133.7 versus 84.2 mIU/ml and 16.0 versus 6.2 mIU/ml for >30 repeats versus <30 repeats respectively. Inhibin B concentrations were not associated with CGG repeats. The results of this study indicate that FMR1 premutations are rare in sporadic cases of POF with no family history of fragile X syndrome. However, although in the normal polymorphic range, expansion of the CGG repeat tract to beyond 30 repeats was associated with serum FSH concentrations in both POF cases and controls.
authors.affiliation: Department of Reproductive Endocrinology and Infertility, National Institute for Research in Reproductive Health (ICMR), Parel, Mumbai 400 012, India.
authors.forename: Suparna
authors.initials: S
authors.lastname: Chatterjee
authors.forename: Anurupa
authors.initials: A
authors.lastname: Maitra
authors.forename: Seema
authors.initials: S
authors.lastname: Kadam
authors.forename: Zarine
authors.initials: Z
authors.lastname: Patel
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Gokral
authors.forename: Pervin
authors.initials: P
authors.lastname: Meherji
mesh_terms: D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics*; D006801:Humans; D007194:India; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016649:Primary Ovarian Insufficiency / Q000235:genetics*; D018911:Trinucleotide Repeats
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1; C497683:FMR1 protein, human