[An association study of abdominal obesity and polymorphisms of UCP2 and SREBP1c genes].
OBJECTIVE
To investigate the association of -866A/G polymorphism of uncoupling protein 2 (UCP2) gene, and 54G/C polymorphism of sterol regulatory element binding protein 1c (SREBP1c) gene with abdominal obesity in the population of type 2 diabetes mellitus families.


METHODS
Eligible type 2 diabetes mellitus cases from newly diagnosed and previous hospitalized patients were choson, then their family members (siblings and parents) tracked. A set of questionnaires was administered to obtain information on demographic characteristics. Physical measurements were recorded. DNA was extracted from blood samples and genotyped using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP). Generalized estimating equation (GEE) was used to adjust for within-family correlation in analysis of relationships of factors.


RESULTS
In the study population, the frequency of A allele of UCP2-866A/G polymorphism was 0.459, and of G allele 0.541; the frequency of G allele of SREBP1c 54G/C polymorphism was 0.822, and of C allele 0.178. Totally 762 participants were analyzed using GEE regression. It was shown that the odds ratio (OR) of the population with only 54G/C polymorphism of SREBP1c gene being the mutant type (GC/CC) was statistically significant while -866A/G polymorphism of UCP2 gene not being the mutant type (AG/GG) was not. The OR of the population with the opposite genotype status was 1.8 (P=0.042), and that with mutant types of both polymorphisms 3.2(P=0.001).


CONCLUSIONS
In the population of type 2 diabetes mellitus families, only 54G/C polymorphism of SREBP1c gene being the mutant type (GC/CC) might be a moderate risk factor of abdominal obesity. When both the two polymorphisms studied are the mutant type, the risk of abdominal obesity may increase significantly.
Song
Yan
Y
Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing 100191, China.
Li
Na
N
He
Liu
L
Chen
Qing
Q
Tang
Xun
X
Chen
Da-fang
DF
Wang
Jin-wei
JW
Dou
Hui-dong
HD
Liu
Hong-dan
HD
Hu
Yong-hua
YH
D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics*; D005192:Family Health; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics*; D056128:Obesity, Abdominal / Q000150:complications / Q000235:genetics*; D011110:Polymorphism, Genetic; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*; D000071246:Uncoupling Protein 2
D007473:Ion Channels; D024101:Mitochondrial Proteins; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2