title: Changes in NGF/c-Fos colocalization in specific limbic structures of juvenile and aged rats after open field stimulation.
abstract: Changes in NGF release during stressful events have been associated with the activation of neurons expressing NGF receptors. This study examined the influence of acute stress-induced stimulation on NGF/c-Fos colocalization in the following limbic regions: the paraventricular (PV) nucleus of the hypothalamus, medial (MeA) nucleus of the amygdala, and CA3 hippocampus. Juvenile (P21) and aged rats (P360) were exposed to a 15-minute acute open field (OF) test. Double immunofluorescence staining, used to detect NGF-ir and c-Fos-ir cells, revealed a higher percentage of NGF/c-Fos-ir neurons in the P21 control group than in the P360 control group. Under OF acute stimulation, a statistically significant (p < 0.05) increase of NGF/c-Fos level in CA3 of juvenile animals and in PV and CA3 of the aged rats was observed. These observations indicate that the investigated structures in both age groups show a different response to acute OF stimulation. Acute OF affects the levels of NGF/c-Fos more significantly in aged rats.
authors.affiliation: Department of Anatomy and Neurobiology, Medical University of Gdańsk, Poland. ewabadowska@amg.gda.pl
authors.forename: E
authors.initials: E
authors.lastname: Badowska-Szalewska
authors.forename: I
authors.initials: I
authors.lastname: Klejbor
authors.forename: T
authors.initials: T
authors.lastname: Cecot
authors.forename: B
authors.initials: B
authors.lastname: Domaradzka-Pytel
authors.forename: B
authors.initials: B
authors.lastname: Ludkiewicz
authors.forename: J
authors.initials: J
authors.lastname: Moryś
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism* / Q000523:psychology; D000679:Amygdala / Q000378:metabolism / Q000503:physiopathology; D000818:Animals; D001931:Brain Mapping; D056654:CA3 Region, Hippocampal / Q000378:metabolism / Q000503:physiopathology; D005455:Fluorescent Antibody Technique; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism / Q000503:physiopathology; D008032:Limbic System / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism / Q000503:physiopathology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D020932:Nerve Growth Factor