[Cloning and tissue distribution analysis of CD1d in Rhesus macaque].
OBJECTIVE
To amplify the CD1d and analyze its tissue distribution in Rhesus macaque.


METHODS
Extracting total RNA from different tissues of Rhesus macaque. CD1d amplification was carried using specific primers and tissue distribution was analyzed by semi-quantification RT-PCR, using the house-keeping gene GAPDH as an internal control.


RESULTS
The CD1d coding region was obtained in this experiment and tissue distribution was analyzed: a highest level of CD1d expression was detected in heart, liver and spleen, lower level in blood, lung, small intestine and stomach, and least in brain.


CONCLUSIONS
The successful amplification of CD1d is useful to produce anti-CD1d antibody and CD1d-tetramer to study NKT cells in Rhesus macaque. The expression profile of CD1d mRNA in Rhesus macaque is in accordance with the tissue distribution and the functions of NKT cells in other species. These results will provide important insights for future research on the relationship of CD1d/NKT cells and their roles in different tissues.
Zhang
Ping
P
Overseas Creative Team, Kunming Institute of Zoology, Kunming 650223, China. zhangping5021@163.com
Zhou
Hui-fang
HF
Sun
Zheng-hua
ZH
Shao
Xiao-li
XL
Zhang
Hua-tang
HT
Xu
Xiao-ning
XN
D000818:Animals; D055610:Antigens, CD1d / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D008253:Macaca mulatta / Q000276:immunology*; D055611:Natural Killer T-Cells / Q000276:immunology; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis
D055610:Antigens, CD1d; D012333:RNA, Messenger