title: Marine n-3 polyunsaturated fatty acids in adipose tissue and breast cancer risk: a case-cohort study from Denmark.
abstract: OBJECTIVE
  The aim of this study was to investigate the association between the content of marine n-3 polyunsaturated fatty acids (PUFA) in adipose tissue, a biomarker for the long-term intake of seafood, and the subsequent development of breast cancer (BC).
  
  
  METHODS
  We designed a case-cohort study based on a cohort of healthy Danish women, who in the 1990 s donated adipose tissue biopsies to a biobank in order to investigate the role of diet for the development of cancer and chronic disease. During follow-up, incident cases of BC were identified through national registries, and the content of n-3 PUFA in adipose tissue was compared between cases and the cohort sample.
  
  
  RESULTS
  During follow-up, 463 new cases of BC were identified. After adjusting for potential confounders, no significant association between the content of marine n-3 PUFA and BC was found. When comparing the highest with the lowest quintile, the hazard ratio (HR) was 0.96 (95% CI 0.64-1.43) for total marine n-3 PUFA, 0.84 (95% CI 0.58-1.23) for eicosapentaenoic acid (EPA), and 1.08 (95% CI 0.73-1.58) for docosahexaenoic acid (DHA).
  
  
  CONCLUSIONS
  This study does not indicate any association between the content of total or individual marine n-3 PUFA in adipose tissue and development of BC.
authors.affiliation: Department of Oncology, Aalborg Hospital, Aarhus University Hospital, Hobrovej 18-22, 9100, Aalborg, Denmark. p.witt@rn.dk
authors.forename: Petra M
authors.initials: PM
authors.lastname: Witt
authors.forename: Jeppe H
authors.initials: JH
authors.lastname: Christensen
authors.forename: Erik B
authors.initials: EB
authors.lastname: Schmidt
authors.forename: Claus
authors.initials: C
authors.lastname: Dethlefsen
authors.forename: Anne
authors.initials: A
authors.lastname: Tjønneland
authors.forename: Kim
authors.initials: K
authors.lastname: Overvad
authors.forename: Marianne
authors.initials: M
authors.lastname: Ewertz
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D016022:Case-Control Studies; D003718:Denmark; D015525:Fatty Acids, Omega-3 / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D015525:Fatty Acids, Omega-3