title: Dopamine D2 receptor mRNA expression is increased in the jugular-nodose ganglia of rats with nitrogen dioxide-induced chronic bronchitis.
abstract: The bronchodilatatory effect of inhaled dopamine or dopamine D(2) receptor agonists in cases of bronchial constriction may involve the suppression of pathologically increased airway sensory nerve activity. The aim of this study is to investigate the regulation of the dopamine D(2) receptor mRNA expression in the ganglia of rats with nitrogen dioxide-induced chronic bronchitis compared with that in ganglia of healthy control animals. Rats were exposed to nitrogen dioxide (10 ppm, 20 d) and dopamine D(2) receptor mRNA levels in sensory ganglia (jugular-nodose, trigeminal, cervical dorsal root and thoracic dorsal root ganglia) were examined by quantitative real-time polymerase chain reaction and compared to control tissues. Whereas for trigeminal and dorsal root ganglia the dopamine D(2) receptor expression levels showed no difference between both animal groups, there was a significant (p<0.05) increase in the jugular-nodose ganglia with a 2.1-fold factor. The increase of dopamine D(2) receptor mRNA in jugular-nodose sensory neurons which innervate the airways may represent a neurochemical basis for the effects seen in man and animal models following topical administration of dopamine or dopamine agonists onto the respiratory epithelium.
authors.affiliation: Department of Pediatric Pneumology and Immunology, Charité-Campus Virchow, Medical University Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. christian.peiser@charite.de
authors.forename: Christian
authors.initials: C
authors.lastname: Peiser
authors.forename: Doerthe A
authors.initials: DA
authors.lastname: Andreae
authors.forename: David A
authors.initials: DA
authors.lastname: Groneberg
authors.forename: Q Thai
authors.initials: QT
authors.lastname: Dinh
authors.forename: Bernd
authors.initials: B
authors.lastname: Müller
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wahn
authors.forename: Axel
authors.initials: A
authors.lastname: Fischer
mesh_terms: D000818:Animals; D001991:Bronchitis / Q000139:chemically induced / Q000378:metabolism*; D002574:Cervical Vertebrae; D002908:Chronic Disease; D005727:Ganglia, Spinal / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009585:Nitrogen Dioxide; D009620:Nodose Ganglion / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013904:Thoracic Vertebrae; D012668:Trigeminal Ganglion / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D009585:Nitrogen Dioxide