A generalized numerical approach to steady-state enzyme kinetics: applications to protein kinase inhibition.
A generalized numerical treatment of steady-state enzyme kinetics is presented. This new approach relies on automatic computer derivation of the underlying mathematical model (a system of simultaneous nonlinear algebraic equations) from a symbolic representation of the reaction mechanism (a system of biochemical equations) provided by the researcher. The method allows experimental biochemists to analyze initial-rate enzyme kinetic data, under the steady-state approximation, without having to use any mathematical equations. An illustrative example is based on the inhibition kinetics of p56(lck) kinase by an ATP competitive inhibitor. A computer implementation of the new method, in the modified software package DYNAFIT [Kuzmic, P. (1996) Anal. Biochem. 237, 260-273], is freely available to all academic researchers.
Kuzmic
Petr
P
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D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D047428:Protein Kinase Inhibitors / Q000737:chemistry*
D047428:Protein Kinase Inhibitors; D000255:Adenosine Triphosphate; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)