title: Effect of polymorphisms in the FASN, OLR1, PPARGC1A, PRL and STAT5A genes on bovine milk-fat composition.
abstract: The aim of our study was to estimate effects of polymorphisms in the ATP-binding cassette G2 (ABCG2), fatty acid synthase (FASN), oxidized low-density lipoprotein receptor 1 (OLR1), peroxysome proliferator-activated receptor-gamma coactivator-1alpha (PPARGC1A), prolactin (PRL) and signal transducer and activator of transcription 5A (STAT5A) genes on milk production traits and detailed milk-fat composition. Milk-fat composition phenotypes were available for 1905 Dutch Holstein-Friesian cows. First, the presence of each SNP in the Dutch Holstein-Friesian population was evaluated by direct sequencing of the PCR product surrounding the SNP in 22 proven Dutch Holstein-Friesian bulls. The ABCG2 SNP did not segregate in the bull population. Second, we genotyped the cows for the FASN(g.16024G>A), FASN(g.17924A>G), OLR1(g.8232C>A), PPARGC1A(c.1790+514G>A), PPARGC1A(c.1892+19G>A), PRL(g.8398G>A) and STAT5A(g.9501G>A) polymorphisms, and estimated genotype effects on milk production traits and milk-fat composition. FASN(g.17924A>G) and OLR(g.8232C>A) had a significant effect (P < 0.05) on milk-fat percentage. However, we were not able to confirm results reported in the literature that showed effects of all evaluated polymorphisms on milk-fat percentage or milk-fat yield. All polymorphisms showed significant effects (P < 0.05) on milk-fat composition. The polymorphisms in FASN and STAT5A, which had an effect on C14:0 and were located on chromosome 19, could not fully explain the quantitative trait locus for C14:0 that was previously detected on chromosome 19 in a genome-wide scan using linkage analysis.
authors.affiliation: Animal Breeding and Genomics Centre, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands. aschennink@ucdavis.edu
authors.forename: A
authors.initials: A
authors.lastname: Schennink
authors.forename: H
authors.initials: H
authors.lastname: Bovenhuis
authors.forename: K M
authors.initials: KM
authors.lastname: Léon-Kloosterziel
authors.forename: J A M
authors.initials: JA
authors.lastname: van Arendonk
authors.forename: M H P W
authors.initials: MH
authors.lastname: Visker
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D005223:Fats / Q000032:analysis*; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci
chemical_list: D005223:Fats