Identification and functional study of a shrimp Dorsal homologue.
Rel/NF-kappaB transcription factors play central roles in induction and regulation of innate immune responses. Here, identification and functional analysis of LvDorsal, a Dorsal homologue from the Pacific white shrimp Litopenaeus vannamei, were described. The full-length cDNA of LvDorsal is 2204bp with an open reading frame that encodes 400 amino acids. The deduced LvDorsal contains a conserved Rel homology domain (RHD), an IPT (Ig-like, plexins and transcription factors) domain and a nucleus localization signal, suggesting that it belongs to the class II NF-kappaB. RT-PCR analysis showed that LvDorsal mRNAs were expressed in all the tissues tested, including gill, epidermis, hemocytes, intestine, stomach, eyestalk, brain, hepatopancreas, muscle, heart and pyloric caecum. Immunofluorescence assay showed that recombinant LvDorsal was translocated into the nucleus of Drosophila S2 cells. Electrophoretic mobility shift assay illustrated that recombinant LvDorsal RHD from S2 cells bound specifically with D. melanogaster kappaB motifs. Additionally, the dual-luciferase reporter assays indicated that LvDorsal could transactivate the reporter gene controlled by the 5' flanking region of shrimp penaeidin-4 and Drosophila attacin genes, suggesting that LvDorsal can regulate the transcription of shrimp penaeidin-4 gene. Study of LvDorsal will help us to better understand shrimp immunity and may help to obtain more effective methods to prevent shrimp diseases.
Huang
Xian-De
XD
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou, PR China.
Yin
Zhi-Xin
ZX
Jia
Xiao-Ting
XT
Liang
Jian-ping
JP
Ai
Hua-Shui
HS
Yang
Li-Shi
LS
Liu
Xi
X
Wang
Pei-Hui
PH
Li
Se-dong
SD
Weng
Shao-Ping
SP
Yu
Xiao-Qiang
XQ
He
Jian-Guo
JG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D033364:Decapoda / Q000737:chemistry / Q000276:immunology*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D010802:Phylogeny; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D016328:NF-kappa B; D014157:Transcription Factors