title: High-performance thin-layer chromatographic determination of rabeprazole sodium and domperidone in combined dosage form.
abstract: A new, simple, high-performance thin-layer chromatographic method for determination of rabeprazole sodium (RAB) and domperidone (DOM) in combined tablet dosage form has been developed and validated. The mobile phase was toluene-acetone-methanol (4.5 + 4.5 + 0.5, v/v/v) with UV detection at 285 nm. The retention factors for RAB and DOM were found to be 0.53 +/- 0.12 and 0.32 +/- 0.20. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 50-800 ng/band for both RAB and DOM. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
authors.affiliation: AISSMS College of Pharmacy, Department of Pharmaceutical Analysis, Pune, India. santoshvgandhi@rediffmail.com
authors.forename: Santosh Vilashchand
authors.initials: SV
authors.lastname: Gandhi
authors.forename: Samina I
authors.initials: SI
authors.lastname: Khan
authors.forename: Rashmi T
authors.initials: RT
authors.lastname: Jadhav
authors.forename: Saket S
authors.initials: SS
authors.lastname: Jadhav
authors.forename: Gaurav A
authors.initials: GA
authors.lastname: Jadhav
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis*; D000932:Antiemetics / Q000032:analysis*; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002855:Chromatography, Thin Layer; D004294:Domperidone / Q000032:analysis*; D004338:Drug Combinations; D007202:Indicators and Reagents; D054328:Proton Pump Inhibitors / Q000032:analysis*; D064750:Rabeprazole; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000932:Antiemetics; D004338:Drug Combinations; D007202:Indicators and Reagents; D054328:Proton Pump Inhibitors; D013607:Tablets; D064750:Rabeprazole; D004294:Domperidone