title: Single europium-doped nanoparticles measure temporal pattern of reactive oxygen species production inside cells.
abstract: Low concentrations of reactive oxygen species, notably hydrogen peroxide (H(2)O(2)), mediate various signalling processes in the cell. Production of these signals is highly regulated and a suitable probe is needed to measure these events. Here, we show that a probe based on a single nanoparticle can quantitatively measure transient H(2)O(2) generation in living cells. The Y(0.6)Eu(0.4)VO(4) nanoparticles undergo photoreduction under laser irradiation but re-oxidize in the presence of oxidants, leading to a recovery in luminescence. Our probe can be regenerated and reliably detects intracellular H(2)O(2) with a 30-s temporal resolution and a dynamic range of 1-45 microM. The differences in the timing of intracellular H(2)O(2) production triggered by different signals were also measured using these nanoparticles. Although the probe is not selective towards H(2)O(2), in many signalling processes H(2)O(2) is, however, the dominant oxidant. In conjunction with appropriate controls, this probe is a powerful tool for unravelling pathways that involve reactive oxygen species.
authors.affiliation: Laboratoire d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM U696, Palaiseau, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Casanova
authors.forename: Cédric
authors.initials: C
authors.lastname: Bouzigues
authors.forename: Thanh-Liêm
authors.initials: TL
authors.lastname: Nguyên
authors.forename: Rivo O
authors.initials: RO
authors.lastname: Ramodiharilafy
authors.forename: Latifa
authors.initials: L
authors.lastname: Bouzhir-Sima
authors.forename: Thierry
authors.initials: T
authors.lastname: Gacoin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Boilot
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Tharaux
authors.forename: Antigoni
authors.initials: A
authors.lastname: Alexandrou
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002478:Cells, Cultured; D005063:Europium / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D015336:Molecular Probe Techniques; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000032:analysis*
chemical_list: D017382:Reactive Oxygen Species; D005063:Europium; D006861:Hydrogen Peroxide