title: Proteome analysis of biomarkers in the cerebrospinal fluid of neuromyelitis optica patients.
abstract: OBJECTIVE
  To better understand the pathophysiological mechanisms underlying neuromyelitis optica (NMO), we developed a proteomics platform for biomarker discovery in the cerebrospinal fluid (CSF) of patients with NMO.
  
  
  METHODS
  Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) were used to compare the CSF proteome of NMO patients with that of controls. A subsequent ELISA and western blot analysis were performed to verify the results of the proteomic analysis. Pathway Studio 5.0 software was used to determine possible functional interactions among these differentially expressed proteins.
  
  
  RESULTS
  Using 2-DE and MALDI-TOF MS, we identified 11 differentially expressed proteins and two isoforms of these same proteins. The expression of four proteins was enhanced, whereas the expression of seven proteins was reduced in the NMO group in comparison to the control group. These differences in protein expression were confirmed by performing ELISA and western blot analyses (p<0.01). Protein network analyses revealed biologic interactions and cross-talks among these differentially expressed proteins.
  
  
  CONCLUSIONS
  Because of their unique expression profile in NMO CSFs, these proteins are candidate biomarkers for NMO. Thus, our findings may have important implications for both the diagnosis of NMO and the further understanding of its pathogenesis.
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Shandong 250012, People's Republic of China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Bai
authors.forename: Shilian
authors.initials: S
authors.lastname: Liu
authors.forename: Xuxiao
authors.initials: X
authors.lastname: Guo
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Qin
authors.forename: Banqin
authors.initials: B
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.forename: Yanjiang
authors.initials: Y
authors.lastname: Qin
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Liu
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009471:Neuromyelitis Optica / Q000134:cerebrospinal fluid*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D015203:Reproducibility of Results; D014809:Vitamin D-Binding Protein / Q000378:metabolism
chemical_list: D015415:Biomarkers; D006242:Haptoglobins; D010455:Peptides; D020033:Protein Isoforms; D020543:Proteome; D014809:Vitamin D-Binding Protein