Activity of novel oxazolidinones against Nocardia brasiliensis growing within THP-1 macrophages.
BACKGROUND
Nocardia are organisms that can escape the effects of both immune response and antimicrobial agents, due to their potential capacity to grow intracellularly. In previous studies, we found that experimental oxazolidinones, DA-7157 and DA-7218, are active both in vitro and in vivo.


OBJECTIVE
In this study, we compare the ability of linezolid, DA-7157 and DA-7218 to inhibit intracellular growth of Nocardia brasiliensis within the human monocyte cell line THP-1.


RESULTS
The addition of oxazolidinones to the infected macrophage monolayer at concentrations 0.25x, 1x, 4x and 16x the MIC for N. brasiliensis resulted in an inhibitory effect on bacterial growth as follows DA-7157 > or = DA-7218 > linezolid.


CONCLUSIONS
The excellent intracellular antimicrobial activity detected suggests that these compounds could be effective in the treatment of actinomycetoma. However, more studies are needed both in vitro and in vivo, including clinical trials, to confirm this issue.
Vera-Cabrera
Lucio
L
Servicio de Dermatología, Hospital Universitario José E. González, Monterrey, N.L., México. luvera_99@yahoo.com
Espinoza-González
Nelly A
NA
Welsh
Oliverio
O
Ocampo-Candiani
Jorge
J
Castro-Garza
Jorge
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D015169:Colony Count, Microbial / Q000379:methods; D003600:Cytosol / Q000382:microbiology*; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009615:Nocardia / Q000187:drug effects* / Q000254:growth & development*; D010755:Organophosphates / Q000494:pharmacology*; D010080:Oxazoles / Q000494:pharmacology*; D013777:Tetrazoles / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; C515039:DA 7157; D010755:Organophosphates; D010080:Oxazoles; D013777:Tetrazoles; C515040:tedizolid phosphate