[Construction and expression of human IL-8 sense or antisense eukaryotic expression vectors].
OBJECTIVE
To construct the sense or antisense IL-8 eukaryotic expression vectors.


METHODS
Sense or antisense IL-8 full length gene were amplified by RT-PCR and cloned into eukaryotic expression vector pcDNA3.1(+). After the identification by PCR, restriction endonuclease digestion and the nucleotide sequencing, the recombinant vectors were transfected into human ovarian carcinoma A2780 and SKOV3 cell lines transiently by lipofectamine mediation. The expression of IL-8 gene and protein were detected by RT-PCR and ELISA.


RESULTS
The sense or antisense IL-8 eukaryotic expression vectors were constructed and verified. The expression of IL-8 gene and protein in A2780 cells transfected with pcDNA3.1(+)-ssIL-8 were increased, whereas the expression of IL-8 protein in SKOV3 cells transfected with pcDNA3.1(+)-asIL-8 was decreased.


CONCLUSIONS
The eukaryotic expression vectors pcDNA3.1(+)-ssIL-8 or pcDNA3.1(+)-asIL-8 have been constructed successfully, which lays a base for further study on roles of IL-8 in ovarian cancer and other tumors.
Niu
Xiu-long
XL
Department of Immunology, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China. nxlwi_ren@126.com
Wang
Yue
Y
Qu
Ye
Y
Guo
Xiao-qin
XQ
Ye
Lu
L
D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism
D016373:DNA, Antisense; D016209:Interleukin-8