Effect of a dentifrice containing the peptide of streptococcal antigen I/II on the adherence of mutans streptococcus.
OBJECTIVE
Antigen I/II of mutans streptococcus (MS) is a surface protein, which binds to salivary receptors. The objective of this study was to study the effect of a peptide corresponding to residues 1025-1044 of antigen I/II and a dentifrice containing peptide on the adherence of MS.


METHODS
The peptide was synthesized and included into dentifrice. Anti-adherence experiments of the peptide and a dentifrice containing it were processed in vitro and in vivo.


RESULTS
The results showed that the adherence of MS to hydroxyapatite was inhibited by synthetic antigen I/II peptide and also by a dentifrice containing the peptide in vitro. The numbers of MS in plaque from subjects treated with dentifrices containing the peptide for 1 month was significantly reduced compared to baseline (log 3.11+/-0.80 vs log 4.09+/-0.90, P<0.01), whereas the control group showed no reduction.


CONCLUSIONS
The adherence of MS to hydroxyapatite was inhibited by both a synthetic peptide and a dentifrice containing the peptide. The peptide dentifrice produced a significant reduction of MS in dental plaque in vivo.
Li
Ming-Yu
MY
Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Medical College, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai 200011, PR China.
Wang
Jun
J
Lai
Guang-Yun
GY
D000328:Adult; D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018592:Cross-Over Studies; D044622:Dental Pellicle / Q000382:microbiology; D003773:Dental Plaque / Q000188:drug therapy / Q000382:microbiology; D003802:Dentifrices / Q000737:chemistry / Q000494:pharmacology*; D004311:Double-Blind Method; D017886:Durapatite; D005260:Female; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects*; D055815:Young Adult
D000942:Antigens, Bacterial; D003802:Dentifrices; D010446:Peptide Fragments; D017886:Durapatite