Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers.
The micronucleus-centromere assay using a pan-centromeric probe was used to assess chromosomal damage in lymphocytes of 47 industrial radiographers occupationally exposed to low dose ionizing radiation and 47 controls. The influence of genotype of DNA repair genes (XRCC1(399), XRCC3(241) and XPD(751)) on micronuclei (MN) frequency was also investigated. Centromere negative micronuclei (MNC-) frequency was significantly higher in radiographers than in controls, whereas similar centromere positive micronuclei (MNC+) frequency was observed in both groups. Poisson regression analyses revealed that the MNC- frequency was significantly associated with radiation occupational exposure and with cumulative-radiation doses in radiographers, after adjusting for confounding variables such as age, smoking, alcohol intake and genotypes. Compared to homozygous wild-type subjects, MNC- frequency in radiographers with variant XRCC3 genotype was significantly higher using univariate analysis. There were no differences in MNC- or MNC+ frequencies by genotype in controls. In conclusion, scoring of MNC- is a useful cytogenetic biomonitoring method for radiographers. Polymorphisms in XRCC3 might contribute to the increased genetic damage in individuals occupationally exposed to chronic ionizing radiation.
Cho
Yoon Hee
YH
Department of Molecular Epidemiology, School of Public Health, Seoul National University, Chongno-ku, Seoul 110-460, Republic of Korea.
Kim
Yang Jee
YJ
An
You Sun
YS
Woo
Hae Dong
HD
Choi
Soo Yong
SY
Kang
Chang Mo
CM
Chung
Hai Won
HW
D000328:Adult; D002503:Centromere / Q000528:radiation effects*; D004260:DNA Repair / Q000235:genetics / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D015162:Micronucleus Tests; D016273:Occupational Exposure / Q000009:adverse effects*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D011834:Radiation Monitoring; D011859:Radiography
D004268:DNA-Binding Proteins