[Neutrophil surface expression of adhesion molecule CD11b in patients with type 2 diabetes].
OBJECTIVE
1. Assessment of cell surface expression of adhesion molecule CD11b on peripheral blood neutrophils in patients with type 2 diabetes without vascular complications. 2. Analysis of serum levels of soluble adhesion molecules: E-selectin (sE-selectin), soluble Intercellular Adhesion Molecule-1 (sICAM-1), soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) and von Willebrand Factor (vWF). 3. Evaluation of systemic inflammatory markers: Interleukin-6 (IL-6), soluble Interleukin-6 Receptor (IL-6Rs), high sensitivity C-Reactive Protein (hsCRP), fibrinogen and leptin.


METHODS
22 patients with type 2 diabetes were enrolled in the study (10-female, 12-male), aged from 40 to 65 yrs, diabetes duration mean 5.32 +/- 1.70 yrs. The control group included 20 healthy volunteers. Flow cytometry was used to analyse neutrophil expression of CD11b. Both inflammatory markers and soluble adhesion molecules were determined by immunoenzymatic assay.


RESULTS
Neutrophil surface CD11b expression did not vary between groups. Significantly higher concentrations of sE-selectin, hsCRP, leptin and IL-6 were found in diabetic patients in comparison with the control group, sICAM-1, sVCAM-1, fibrinogen, vWF and IL-6Rs concentrations did not significantly vary between groups. In the diabetic group--a positive correlation was established between neutrophil CD11b expression and hsCRP, HOMA IR, BMI, leptin and fibrinogen.


CONCLUSIONS
Type 2 diabetes without vascular complications is not accompanied by increase in CD11b expression on peripheral blood neutrophils. The degree of neutrophil activation is correlated with an increase in leptin serum levels. Obtained results confirm mutual connections between obesity, type 2 diabetes and chronic inflammatory process.
Mastej
Krzysztof
K
Katedra i Klinika Angiologii, Nadciśnienia, Tetniczego i Diabetologii Akademii Medycznej we Wrocławiu. kmastej@interia.pl
Adamiec
Rajmund
R
D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D039481:CD11b Antigen / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000276:immunology*; D019040:E-Selectin / Q000097:blood; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*
D039481:CD11b Antigen; D019040:E-Selectin; C509295:ITGAM protein, human; D015850:Interleukin-6; D020738:Leptin; D002097:C-Reactive Protein