Optimization of the enzymatic production of 20(S)-ginsenoside Rg(3) from white ginseng extract using response surface methodology.
The optimization of the conversion of ginseng saponin glycosides to 20(S)-ginsenoside Rg(3) by enzymatic transformation was carried out using response surface methodology (RSM) based on a 2(3) factorial central composite design. The production of 20(S)-ginsenoside Rg(3) using several commercial enzymes indicated that the enzyme Cellulase-12T was the most efficient at producing 20(S)-ginsenoside Rg(3). To optimize the enzymatic production of 20(S)-ginsenoside Rg(3), response surface methodology was applied to determine the ideal amount of white ginseng extract, Cellulase-12T and reaction time. These results indicate that white ginseng extract (1.67%) treated with Celluase-12T (3.67%) for 72 hours had 4 times the quantity of 20(S)-ginsenoside Rg(3) compared to commercial white ginseng extract.
Chang
Kyung-Hoon
KH
Division of Animal Life Science, Konkuk University, Seoul 143-701, Republic of Korea.
Jee
Hee Sook
HS
Lee
Na-Kyoung
NK
Park
Se-Ho
SH
Lee
Na-Won
NW
Paik
Hyun-Dong
HD
D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000096:biosynthesis* / Q000737:chemistry; D016018:Least-Squares Analysis; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000378:metabolism*; D012044:Regression Analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
D036145:Ginsenosides; D010936:Plant Extracts; C097367:ginsenoside Rg3; D002480:Cellulase