Sperm meiotic segregation and aneuploidy in a 46,X,inv(Y),t(10;15) carrier: case report.
OBJECTIVE
To analyze the meiotic segregation and an interchromosomal effect in sperm of an inv(Y) (p11.1;q11.2),t(10;15) (q25.2;q12) carrier.


METHODS
Case report.


METHODS
Research institute.


METHODS
Man with a karyotype 46,X,inv(Y),t(10;15), normal sperm parameters, and secondary infertility.


METHODS
Multicolor fluorescence in situ hybridization using probes for chromosomes 10, 15, 8, 18, 21, X, and Y.


METHODS
Frequencies of meiotic segregation products and aneuploidy of chromosomes 8, 18, 21, X, and Y.


RESULTS
The most frequent type of meiotic segregation was the alternate (40.82%), followed by the adjacent 1 (28.09%), adjacent 2 (16.33%), and 3:1 (9.91%) segregations. Neither deviation from the expected 1:1 ratio of the X- and Y-bearing spermatozoa nor any evidence of an interchromosomal effect on aneuploidy of chromosomes X, Y, 8, 18, and 21 and diploidy was observed in the carrier compared with control donors. The disomies of chromosomes 8 and 21 were equally frequent in X- and Y-bearing spermatozoa of the carrier.


CONCLUSIONS
The fluorescence in situ hybridization analysis of meiotic segregation and aneuploidy helps to personalize the reproductive risk in carriers of balanced structural chromosomal aberrations. Complete information concerning the quality of spermatogenesis is necessary in all donors (both translocation carriers and controls) implicated in interchromosomal effect studies.
Vozdova
Miluse
M
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic. vozdova@vri.cz
Oracova
Eva
E
Gaillyova
Renata
R
Rubes
Jiri
J
D000328:Adult; D000782:Aneuploidy; D016022:Case-Control Studies; D007446:Chromosome Inversion; D020090:Chromosome Segregation / Q000235:genetics / Q000502:physiology; D002879:Chromosomes, Human, Pair 10; D002884:Chromosomes, Human, Pair 15; D041322:Chromosomes, Human, Y / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000235:genetics*; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000002:abnormalities / Q000378:metabolism*; D014178:Translocation, Genetic; D055815:Young Adult