Identification of novel partner proteins of PCBP1.
OBJECTIVE
PCBP1 is a family member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that belong to RNA-binding proteins and bear three KH domains. The protein plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. We hypothesized and were going to identify that the regulatory function of PCBP1 is performed through different complexes of proteins that include PCBP1.


METHODS
To test our hypothesis, approaches of protein walking with a yeast two-hybrid system (Y2H), pulling down in yeasts, co-immunoprecipitation and immunofluorescent microscopy assay were employed in this study. The PCBP1 was used as the initial "walker" to search for its interaction partner(s).


RESULTS
Candidate proteins including MYL6, PECAM1, CSH1, RAB7, p57KIP2, ACTG1, RBMS1 and PSG4-like were identified with selection mediums and preceding methods.


CONCLUSIONS
With these candidate protein molecules, some protein complexes associating with PCBP1 are proposed, which may help in a better understanding of physiological functions of PCBP1 and proved evidence that PCBP1 is involved in variant biological pathways.
Huo
Li-rong
LR
Peking University Center of Medical Genetics, Beijing 100191, China.
Shen
Chen
C
Ju
Wei-na
WN
Zou
Jun-hua
JH
Yan
Wu
W
Brown
W Ted
WT
Zhong
Nanbert
N
D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
D050761:Cyclin-Dependent Kinase Inhibitor p57; D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C094604:PCBP1 protein, human; C086384:RBMS1 protein, human; D016601:RNA-Binding Proteins; D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; D020691:rab GTP-Binding Proteins