How an improved immunoassay sensitivity can be achieved by gamma irradiation: modification, application and characterization of polystyrene surface for anti HIV-1 ELISA.
Polystyrene microtitre plates modified by (60)Co gamma-ray irradiation were used in an indirect enzyme-linked immunosorbent assay (ELISA) for detection of anti-human immunodeficiency virus type 1 (anti HIV-1). The plates with 9 kGy (optimum dose) irradiation showed 2-5-fold higher detection sensitivity in serodiagnosis tests compared to untreated ones, and a 3-fold lower enzyme concentration than the control used was still detectable. Adsorption/desorption experiment results, atomic force microscopy (AFM) images, and X-ray photoelectron spectroscopy (XPS) analysis provide the reason for this improvement. The oxidized surface formed during irradiation presented much more binding affinity for coating antigens and could adsorb a larger amount (1.5-3-fold) of protein uniformly.
Feng
Bo
B
Biological Engineering Department, Xiangtan University, Xiangtan, P. R. China. fengbo@xtu.edu.cn
Dai
Youzhi
Y
Zhang
Hangtuo
H
Wang
Lu
L
Ding
Yanhuai
Y
Zeng
Hongyan
H
D004797:Enzyme-Linked Immunosorbent Assay; D005720:Gamma Rays; D015488:HIV Antigens / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D010084:Oxidation-Reduction; D011137:Polystyrenes / Q000737:chemistry / Q000528:radiation effects*; D012680:Sensitivity and Specificity
D015488:HIV Antigens; D011137:Polystyrenes