title: A role for calcium-calmodulin-dependent protein kinase II in the consolidation of visual object recognition memory.
abstract: The aim was to investigate the role of calcium-calmodulin-dependent protein kinase (CAMK)II in object recognition memory. The performance of rats in a preferential object recognition test was examined after local infusion of the CAMKII inhibitors KN-62 or autocamtide-2-related inhibitory peptide (AIP) into the perirhinal cortex. KN-62 or AIP infused after acquisition impaired memory tested at 24 h, indicating an involvement of CAMKII in the consolidation of recognition memory. Memory was impaired when KN-62 was infused at 20 min after acquisition or when AIP was infused at 20, 40, 60 or 100 min after acquisition. The time-course of CAMKII activation in rats was further examined by immunohistochemical staining for phospho-CAMKII(Thre286)alpha at 10, 40, 70 and 100 min following the viewing of novel and familiar images. At 70 min, processing novel images resulted in more phospho-CAMKII(Thre286)alpha-stained neurons in the perirhinal cortex than did the processing of familiar images, consistent with the viewing of novel images increasing the activity of CAMKII at this time. This difference was eliminated by prior infusion of AIP. These findings establish that CAMKII is active within the perirhinal region between approximately 20 and 100 min following learning and then returns to baseline. Thus, increased CAMKII activity is essential for the consolidation of long-term object recognition memory but continuation of that increased activity throughout the 24 h memory delay is not necessary for maintenance of the memory.
authors.affiliation: Department of Anatomy, Medical Research Council Centre for Synaptic Plasticity, Bristol University, University Walk, Bristol, UK. Chris.Tinsley@bristol.ac.uk
authors.forename: C J
authors.initials: CJ
authors.lastname: Tinsley
authors.forename: K E
authors.initials: KE
authors.lastname: Narduzzo
authors.forename: J W
authors.initials: JW
authors.lastname: Ho
authors.forename: G R
authors.initials: GR
authors.lastname: Barker
authors.forename: M W
authors.initials: MW
authors.lastname: Brown
authors.forename: E C
authors.initials: EC
authors.lastname: Warburton
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives; D000704:Analysis of Variance; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D002452:Cell Count; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005106:Exploratory Behavior / Q000502:physiology; D007150:Immunohistochemistry; D007260:Infusion Pumps; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010455:Peptides / Q000494:pharmacology; D010766:Phosphorylation; D051381:Rats; D021641:Recognition, Psychology / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: C503464:CaMKII inhibitor AIP; D004791:Enzyme Inhibitors; D010455:Peptides; C063302:KN 62; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2