Effects of an endothelial cell-conditioned medium on the hematopoietic and endothelial differentiation of embryonic stem cells.
We have examined the effect of mouse bone marrow endothelial cell-conditioned medium (mEC-CM) on hematopoietic and endothelial differentiation of mouse embryonic stem cells (mESCs). mEC-CM can efficiently promote the differentiation of mESCs into Flk(+) cells and hematopoietic colony-forming cells. mEC-CM proved to be as potent as a cytokine cocktail comprised of VEGF, bFGF, IGF and EGF. After inducing mESCs with mEC-CM, cobblestone-like cells were mechanically selected and identified which had the ability to incorporate DiI-Ac-LDL. DiI-Ac-LDL-positive cells were endothelial-like cells due to their expression of CD31 and Flk1, ability to bind to UEA1 and capacity to form capillary-like tube structures on matrigel. In conclusion, mEC-CM can efficiently promote the differentiation of mESCs into endothelial cells and hematopoietic colony-forming cells. The differentiated endothelial-like cells can be isolated by using DiI-Ac-LDL labeling and mechanical selection.
Sun
Xuan
X
Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, China. almasun97184@gmail.com
Cheng
Lamei
L
Duan
Huaxin
H
Lu
Guangxiu
G
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002196:Capillaries / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D037121:Plant Lectins / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
D017077:Culture Media, Conditioned; D008077:Lipoproteins, LDL; D037121:Plant Lectins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C036850:Ulex europaeus lectins; C032629:acetyl-LDL; D040301:Vascular Endothelial Growth Factor Receptor-2