title: Essential role of STIM1 in the development of cardiomyocyte hypertrophy.
abstract: Store-operated Ca(2+) entry (SOCE) through transient receptor potential (TRP) channels is important in the development of cardiac hypertrophy. Recently, stromal interaction molecule 1 (STIM1) was identified as a key regulator of SOCE. In this study, we examined whether STIM1 is involved in the development of cardiomyocyte hypertrophy. RT-PCR showed that cultured rat cardiomyocytes constitutively expressed STIM1. Endothelin-1 (ET-1) treatment for 48h enhanced TRPC1 expression, SOCE, and nuclear factor of activated T cells activation without upregulating STIM1. However, the knockdown of STIM1 suppressed these effects, thereby preventing a hypertrophic response. These results suggest that STIM1 plays an essential role in the development of cardiomyocyte hypertrophy.
authors.affiliation: Department of Physiology, Akita University Graduate School of Medicine, Akita 010-8543, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Ohba
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Manabu
authors.initials: M
authors.lastname: Murakami
authors.forename: Takako
authors.initials: T
authors.lastname: Sato
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Ono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006332:Cardiomegaly / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000071737:Stromal Interaction Molecule 1; D050052:TRPC Cation Channels / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D002118:Calcium