Stress responses in Prochlorococcus MIT9313 vs. SS120 involve differential expression of genes encoding proteases ClpP, FtsH and Lon.
Prochlorococcus is a marine cyanobacterium responsible for a significant part of global primary production as well as being one of the most abundant organisms on Earth. Protein turnover is an essential and poorly understood aspect of the cyanobacterial response to environmental stresses. In the present work, cultures of the SS120 and MIT9313 strains were subjected to several conditions, and quantitative real time RT-PCR was used to measure changes in the expression of genes encoding three representative ATP-dependent proteases. We found common responses to conditions such as aging. However, the expression pattern under nutrient starvation was strikingly different in the two strains, probably reflecting the different regulatory backgrounds of the two ecotypes here studied.
Gómez-Baena
Guadalupe
G
Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, planta 1, Campus de Rabanales, Universidad de Córdoba, Córdoba 14071, Spain. v52gobag@uco.es
Rangel
Oriol Alberto
OA
López-Lozano
Antonio
A
García-Fernández
Jose Manuel
JM
Diez
Jesús
J
D001426:Bacterial Proteins / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D048929:Prochlorococcus / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013312:Stress, Physiological
D001426:Bacterial Proteins; D010447:Peptide Hydrolases