title: Efficacy of passive immunization with IgY antibodies to a 58-kDa H. pylori antigen on severe gastritis in BALB/c mouse model.
abstract: Consecutive triple doses of 1 x 10(8) CFU/mL of a pathogenic H. pylori strain isolated from stomach of Egyptian patients with severe gastritis were used to establish infection in BALB/c mice model. White Leghorn hens were immunized with H. pylori whole cell lysate (HpLysate) antigen and with a highly reactive 58-kDa H. pylori (Hp58) antigen. Two months later, IgY antibodies (IgY-HpLysate & IgY-Hp58) were purified from egg yolk and its efficacy was evaluated in the adopted model. Microbiological culture and immunohistochemical staining revealed that H. pylori infection was inhibited 1 week after oral passive immunization in 70% of infected BALB/c mice with a significant decrease (p < 0.05) in the degrees of gastritis. In conclusion, we have adapted BALB/c mice model for human H. pylori pathogenic strain and oral passive immunization with specific IgY antibodies to the 58-kDa antigen inhibited active H. pylori infection and decreased gastritis.
authors.affiliation: R&D Department, Biotechnology Research Center, New Damietta City, Egypt. amattallah@hotmail.com
authors.forename: Abdelfattah M
authors.initials: AM
authors.lastname: Attallah
authors.forename: Ayman T
authors.initials: AT
authors.lastname: Abbas
authors.forename: Hisham
authors.initials: H
authors.lastname: Ismail
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Raouf
authors.forename: Ibrahim
authors.initials: I
authors.lastname: El-Dosoky
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D000942:Antigens, Bacterial / Q000276:immunology; D002645:Chickens / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D005756:Gastritis / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D016481:Helicobacter Infections / Q000473:pathology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000276:immunology / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; C016512:IgY; D007136:Immunoglobulins