A highly attenuated measles virus vaccine strain encodes a fully functional C protein.
The P, V, and C proteins of measles virus are encoded in overlapping reading frames of the P gene, which makes it difficult to analyze the functions of the individual proteins in the context of virus infection. We established a system to analyze the C protein independently from the P and V proteins by placing its gene in an additional transcription unit between the H and L genes. Analyses with this system indicated that a highly attenuated Edmonston lineage vaccine strain encodes a fully functional C protein, and the P and/or V protein is involved in the attenuated phenotype.
Nakatsu
Yuichiro
Y
Department of Virology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Takeda
Makoto
M
Iwasaki
Masaharu
M
Yanagi
Yusuke
Y
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D008458:Measles Vaccine / Q000235:genetics*; D008459:Measles virus / Q000235:genetics; D010641:Phenotype; D014613:Vaccines, Attenuated / Q000235:genetics*; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication / Q000502:physiology
D008458:Measles Vaccine; D014613:Vaccines, Attenuated; D017361:Viral Nonstructural Proteins