title: Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component-resolved allergy diagnosis.
abstract: BACKGROUND
  Component-resolved diagnosis (CRD) using microarray technology has recently been introduced into the field of clinical allergology.
  
  
  OBJECTIVE
  To further validate the use of CRD by microarray technology in allergy diagnosis.
  
  
  METHODS
  Thiry-seven patients allergic to birch pollen were included. The discriminative value of apple-specific IgE (sIgE), recombinant Mal d 1 (rMal d 1) sIgE, apple skin prick test and rMal d 1 on the microarray was assessed between patients with a birch-related oral allergy syndrome to apple (OAS(+), n=20) and healthy control individuals (HC, n=8) without a history of inhalant allergies or apple-induced OAS. An additional comparative analysis was carried out with individuals allergic to birch pollen allergy without OAS (OAS(-); n=17).
  
  
  RESULTS
  rMal d 1 coupled to the microarray constitutes a discriminative marker between OAS(+) and HC with a sensitivity 95% and a specificity of 100%. However, in parallel with the traditional sIgE assay, 15 out of 17 OAS(-) individuals (88%) also displayed IgE reactivity to rMal d 1 coupled to the microarray. OAS(-) individuals are more frequently sensitized to mite (about three to four times), cat and dog dander (about two to three times) and grass pollen (about 1.5 times) as compared with OAS(+) patients.
  
  
  CONCLUSIONS
  At first glance, CRD by microarray seems to be a reliable instrument in the diagnosis of apple-mediated OAS in birch pollen allergy. However, for discriminating between sensitization and a real allergy, micro-arrayed rMal d 1 offers no advantage over conventional quantification of rMal d 1 sIgE. Most interestingly, within a single run, birch pollen-allergic patients without OAS to apple were shown to display a broader sensitization to classical inhalant allergens than birch pollen-allergic patients with an apple-related OAS.
authors.affiliation: Departments of Immunology, Allergology, Rheumatology, Faculty of Medicine, University of Antwerp, Antwerpen, Belgium.
authors.forename: D G
authors.initials: DG
authors.lastname: Ebo
authors.forename: C H
authors.initials: CH
authors.lastname: Bridts
authors.forename: M M
authors.initials: MM
authors.lastname: Verweij
authors.forename: K J
authors.initials: KJ
authors.lastname: De Knop
authors.forename: M M
authors.initials: MM
authors.lastname: Hagendorens
authors.forename: L S
authors.initials: LS
authors.lastname: De Clerck
authors.forename: W J
authors.initials: WJ
authors.lastname: Stevens
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D052179:Antigens, Plant / Q000276:immunology*; D029662:Betula / Q000276:immunology*; D002648:Child; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007073:Immunoglobulin E / Q000032:analysis; D008297:Male; D027845:Malus / Q000276:immunology*; D008875:Middle Aged; D011058:Pollen / Q000276:immunology*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012882:Skin Tests; D013577:Syndrome; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D007073:Immunoglobulin E