title: Influence of ethanol on the release of growth factors in human blood-derived platelet gels.
abstract: Platelet gels (PG) are new topical single-donor blood products which are attracting great interest in regenerative medicine. They are obtained by mixing a platelet-rich plasma fraction with thrombin to generate a fibrin gel enriched in platelet growth factors (GF). The type of thrombin preparation may affect PG reproducibility. We have determined the impact of 14.6% (v/v) ethanol-stabilized thrombin (EHT) on the release of GF by platelets. Various ratios of EHT and platelet concentrates were mixed to obtain from 2.43 to 7.96% ethanol concentration. Platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1), vascular endothelium growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were assessed at 5, 120, and 300 min after PG formation. Protein profiles of thrombin and PG releasates were analyzed by SDS-PAGE. The amount of PDGF-AB, TGF-beta1, and VEGF released per platelet decreased significantly (p<0.05) with increasing ethanol concentrations but, however, not that of EGF. IGF-1 content was stable, consistent with its presence mostly in plasma. SDS-PAGE indicated that ethanol did not affect fibrin formation. In conclusion, ethanol has a significant impact on the amount of GF released by platelets and should be strictly controlled to standardize PG and optimize clinical benefits.
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Ya Po
authors.initials: YP
authors.lastname: Kuo
authors.forename: Yueh-Lun
authors.initials: YL
authors.lastname: Lee
authors.forename: Yu Hong
authors.initials: YH
authors.lastname: Tseng
authors.forename: Ching-Hua
authors.initials: CH
authors.lastname: Su
authors.forename: Thierry
authors.initials: T
authors.lastname: Burnouf
authors.forename: Chen Yao
authors.initials: CY
authors.lastname: Su
mesh_terms: D000465:Algorithms; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001772:Blood Cell Count; D001781:Blood Component Removal / Q000379:methods; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology*; D005337:Fibrin / Q000378:metabolism; D005782:Gels; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D013917:Thrombin / Q000378:metabolism
chemical_list: D000891:Anti-Infective Agents, Local; D005782:Gels; D036341:Intercellular Signaling Peptides and Proteins; D012997:Solvents; D000431:Ethanol; D005337:Fibrin; D013917:Thrombin