[Analysis on the expression of the stem cells related genes in hepatocellular carcinoma cell lines].
OBJECTIVE
To investigate the expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines of SMMC-7721, Bel-7402, HepG2, MHCC-97 and normal hepatocellular cell line of L02, and to compare the response of these cell lines to all-trans retinoic acid.


METHODS
RT-PCR was used to detect expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines and normal hepatocellular cell line. Real time-PCR was used to quantify the expression of the genes.


RESULTS
There are different levels of expression of the stem cell-related gene in hepatocellular carcinoma cell lines and control cell line (P less than 0.05). There are significant differences in HepG2 and L-02 for the response to all-trans retinoic acid (P less than 0.05).


CONCLUSIONS
The stem cell-related genes are differentially expressed in different hepatocellular carcinoma cell lines.
Zou
Can
C
Institute of Liver Surgery, Dongfeng Hospital, Yunyang Medical College, Shiyan Hubei 442008, China.
Yuan
Fang-jun
FJ
Zhou
Wen-bo
WB
Huang
Ling
L
Wang
Ju
J
Zhang
You-shun
YS
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D000072086:Smoothened Receptor; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C491023:SMO protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D000072086:Smoothened Receptor; C435831:WNT5B protein, human; D051153:Wnt Proteins; D051176:beta Catenin; D014212:Tretinoin