Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes.
The aim of the present study was to demonstrate the mitogenic and differentiating properties of platelet-rich plasma releasates (PRPr) on human chondrocytes in mono- and three-dimensional cultures. In order to assess if PRPr supplementation could maintain the chondrocyte phenotype or at least inhibit the cell de-differentiation even after several days in culture, we performed a proteomic study on several cell cultures independently grown, for different periods of time, in culture medium with FCS, human serum (HS), and releasates obtained from PRP and platelet-poor plasma (PPP). We found that PRP treatment actually induced in chondrocytes the expression of proteins (some of which novel) involved in differentiation.
Spreafico
Adriano
A
Sezione di Reumatologia, Dipartimento di Medicina Clinica e Scienze Immunologiche, Università di Siena, Policlinico Le Scotte, Siena, Italy. spreafico@unisi.it
Chellini
Federico
F
Frediani
Bruno
B
Bernardini
Giulia
G
Niccolini
Silvia
S
Serchi
Tommaso
T
Collodel
Giulia
G
Paffetti
Alessandro
A
Fossombroni
Vittorio
V
Galeazzi
Mauro
M
Marcolongo
Roberto
R
Santucci
Annalisa
A
D001792:Blood Platelets / Q000378:metabolism; D002358:Cartilage, Articular / Q000378:metabolism*; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D003470:Culture Media; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D010452:Peptide Biosynthesis; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D040901:Proteomics
D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor