title: A first low-resolution difference Fourier map of phosphorus in a membrane protein from near-edge anomalous diffraction.
abstract: Crystal diffraction of three membrane proteins (cytochrome bc(1) complex, sarcoplasmic reticulum Ca(2+) ATPase, ADP-ATP carrier) and of one nucleoprotein complex (leucyl tRNA synthetase bound to tRNAleu, leuRS:tRNAleu) was tested at wavelengths near the X-ray K-absorption edge of phosphorus using a new set-up for soft X-ray diffraction at the beamline ID01 of the ESRF. The best result was obtained from crystals of Ca(2+) ATPase [adenosin-5'-(beta,gamma-methylene) triphosphate complex] which diffracted out to 7 A resolution. Data were recorded at a wavelength at which the real resonant scattering factor of phosphorus reaches the extreme value of -20 electron units. The positions of the four triphosphates of the monoclinic unit cell of the ATPase have been obtained from a difference Fourier synthesis based on a limited set of anomalous diffraction data.
authors.affiliation: ESRF, F-38043 Grenoble, France.
authors.forename: Peter
authors.initials: P
authors.lastname: Boesecke
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Bois
authors.forename: Thibaut
authors.initials: T
authors.lastname: Crépin
authors.forename: Carola
authors.initials: C
authors.lastname: Hunte
authors.forename: Richard
authors.initials: R
authors.lastname: Kahn
authors.forename: Wei Chun
authors.initials: WC
authors.lastname: Kao
authors.forename: Lionel
authors.initials: L
authors.lastname: Nauton
authors.forename: Anne Marie Lund
authors.initials: AM
authors.lastname: Winther
authors.forename: Jesper
authors.initials: J
authors.lastname: Moller
authors.forename: Poul
authors.initials: P
authors.lastname: Nissen
authors.forename: Hughes
authors.initials: H
authors.lastname: Nury
authors.forename: Claus
authors.initials: C
authors.lastname: Olesen
authors.forename: Eva
authors.initials: E
authors.lastname: Pebay-Peyroula
authors.forename: Jean
authors.initials: J
authors.lastname: Vicat
authors.forename: Heinrich
authors.initials: H
authors.lastname: Stuhrmann
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D005583:Fourier Analysis; D008565:Membrane Proteins / Q000737:chemistry*; D010758:Phosphorus / Q000032:analysis*
chemical_list: D008565:Membrane Proteins; D010758:Phosphorus