title: Simultaneous identification and quantitative determination of selected aminoglycoside antibiotics by thin-layer chromatography and densitometry.
abstract: A TLC-densitometric method has been developed for simultaneous identification and quantitative determination of amikacin, gentamicin, kanamycin, neomycin, netilmicin, and tobramycin. This separation of antibiotics was achieved on silica gel TLC plates without a fluorescent indicator and with methanol-25% ammonia-chloroform (3 + 2 + 1, v/v/v) as the mobile phase. The densitometric measurements were made at 500 nm after detection with a 0.2% ninhydrin solution in ethanol. Under these conditions, good separation of the chosen aminoglycosides was obtained. The method is distinguished by high sensitivity, with the LOD from 0.25 microg for amikacin to 1.00 microg for gentamicin and the LOQ from 0.5 microg for amikacin to 1.65 microg for gentamicin, and a wide linearity range 0.75-6.25 microg/spot for amikacin and netilmicin and 1.5-12.50 microg/spot for other antibiotics. The precision of the determination was very good; RSD varied in the range 0.3-0.6%.
authors.affiliation: Collegium Medicum of Jagiellonian University, Department of Inorganic and Analytical Chemistry, Medyczna 9, 30-688 Kraków, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Hubicka
authors.forename: Jan
authors.initials: J
authors.lastname: Krzek
authors.forename: Hanna
authors.initials: H
authors.lastname: Woltyńska
authors.forename: Bozena
authors.initials: B
authors.lastname: Stachacz
mesh_terms: D000617:Aminoglycosides / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D012996:Solutions
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D012996:Solutions