title: Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia.
abstract: Microsporidia are ubiquitous parasites thought to be closely related to fungi. Their presence in the environment means that humans are frequently exposed to infection. Stool samples were collected from 151 indigenous villagers from the eastern state of Pahang in 2005. The samples were concentrated with water-ether sedimentation, stained with modified trichrome stain and examined under oil-immersion microscopy. Thirty-two specimens (21.2%) were positive for microsporidia. Microsporidia were observed as ovoid or rounded ovoid shapes measuring approximately 1mum, with a bright pink outline containing a central or posterior vacuole. PCR amplification with specific primers on microscopy-positive specimens amplified Encephalitozoon intestinalis DNA from five of the ten specimens used.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Angela
authors.initials: A
authors.lastname: Lono
authors.forename: Govind Suresh
authors.initials: GS
authors.lastname: Kumar
authors.forename: Tan Tian
authors.initials: TT
authors.lastname: Chye
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People; D016022:Case-Control Studies; D002648:Child; D017931:DNA Primers; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D016814:Microsporidia / Q000302:isolation & purification; D016881:Microsporidiosis / Q000453:epidemiology* / Q000469:parasitology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013194:Staining and Labeling / Q000379:methods; D055815:Young Adult
chemical_list: D017931:DNA Primers