Acceleration of palatal wound healing in Smad3-deficient mice.
Wound healing is a well-orchestrated complex process leading to the repair of injured tissues. It is suggested that transforming growth factor (TGF)-beta/Smad3 signaling is involved in wound healing. The purpose of this study was to investigate the role of TGF-beta/Smad3 signaling in palatal wound healing in Smad3-deficient (Smad3(-/-)) mice. Histological examination showed that wound closure was accelerated by the proliferation of epithelium and dermal cells in Smad3(-/-) mice compared with wild-type (WT) mice. Macrophage/monocyte infiltration at wounded regions in Smad3(-/-) mice was decreased in parallel with the diminished production of TGF-beta1, monocyte chemoattractant protein-1, and macrophage inflammatory protein-1alpha compared with WT mice. Fibrocytes, expressing hematopoietic surface marker and fibroblast products, were recruited and produced alpha-smooth-muscle actin in WT mice, but were not observed in Smad3(-/-) mice. These results suggest that TGF-beta/Smad3 signaling may play an important role in the regulation of palatal wound healing.
Jinno
K
K
Department of Orthodontics and Dentofacial Orthopedics, Graduate School of Oral Sciences, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan. k.jinno@h7.dion.ne.jp
Takahashi
T
T
Tsuchida
K
K
Tanaka
E
E
Moriyama
K
K
D000199:Actins / Q000032:analysis; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D049109:Cell Proliferation; D018932:Chemokine CCL2 / Q000032:analysis; D054405:Chemokine CCL3 / Q000032:analysis; D002633:Chemotaxis / Q000276:immunology; D004848:Epithelium / Q000503:physiopathology; D005347:Fibroblasts / Q000502:physiology; D006410:Hematopoiesis / Q000276:immunology; D007801:Langerhans Cells / Q000502:physiology; D008264:Macrophages / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009000:Monocytes / Q000502:physiology; D009061:Mouth Mucosa / Q000293:injuries* / Q000503:physiopathology / Q000601:surgery; D010159:Palate / Q000293:injuries* / Q000503:physiopathology / Q000601:surgery; D015398:Signal Transduction / Q000502:physiology; D051900:Smad3 Protein / Q000172:deficiency*; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000502:physiology; D014945:Wound Healing / Q000502:physiology
D000199:Actins; D000954:Antigens, Surface; C496534:Ccl3 protein, mouse; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D016212:Transforming Growth Factor beta