[Expression of AP-1 and TGF-beta1 in kidney of iodine deficient rats and its significance].
OBJECTIVE
To observe the variation in expression of AP-1 and TGF-beta1 in rat kidney of iodine deficiency, to explore the nosogenesis of renal damage in the region of iodine deficiency.


METHODS
The models of deficient iodine rats were established by treatment with low iodine diet, wistar rats were divided into gentle low iodine group(GLIG), dense low iodine group(DLIG) and control group(CL). Serum levels of thyroid humone were determined by chemiluminescent immunoassay. To observe the gene expression of c-Jun by using RT-PCR, observe the expression of TGF-beta1 by using immunohistochemistry.


RESULTS
Compared with CL, the FT3, FT4 of DLIG were significantly decreased(P<0.01), the FT3 of GLIG were significantly decreased(P<0.01), the FT4 of GLIG were not significantly decreased(P>0.05). Compared with GLIG, the FT3, FT4 of DLIG were significantly decreased(P<0.01). Compared with CL, GLIG group, the gene expression of c-Jun was significantly increased(P<0.01) in DLIG group. Compared with CL group, the level of c-Jun was slightly increased(P>0.05)in GLIG group. Compared with CL, the TGF-beta1 of DLIG was significantly increased, the TGF-beta1 of GLIG was significantly increased. Compared with GLIG, the TGF-beta1 of DLIG was significantly increased.


CONCLUSIONS
Iodine deficiency can decrease the secretion of thyroid humone, can lead the activation of c-Jun and TGF-beta1 in kidney. This finding suggest that AP-1 signal transduction pathway participate in the nosogenesis of renal damage.
Dai
Yong-Hong
YH
Yutian County Hospital, Department of Endocrinology, Yutian 064100, China. ytdyh@126.com
Zhang
Jun
J
Liu
Chang-Jie
CJ
Fang
Hui
H
D000818:Animals; D005260:Female; D015870:Gene Expression; D007455:Iodine / Q000172:deficiency*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013963:Thyroid Hormones / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism
D016755:Proto-Oncogene Proteins c-jun; D013963:Thyroid Hormones; D018808:Transcription Factor AP-1; D053773:Transforming Growth Factor beta1; D007455:Iodine