Influence of radiographic contrast media on the secretion of vasoactive substances by primary human umbilical venous endothelial cells (HUVEC): prospective, controlled, in vitro comparative study.
Besides the desirable effect of roentgen ray absorption radiographic contrast media (RCM) can also have varying adverse effects. Under discussion, as a possible cause, are microcirculatory disorders resulting from morphological alterations in erythrocytes and endothelial cells. Therefore, the contrast media-induced release of vasoactive substances (prostacyclin, endothelin-1, and nitric oxide (NO)) from human endothelial cells (HUVEC) induced by two commercially available RCMs (Iodixanol 320, Iomeprol 350) was tested in a controlled in-vitro study. The results show that RCMs lead to endothelial cell trauma in vitro, which is apparent in the release of prostacyclin and endothelin-1, while NO release was not affected. The endothelin-1 release after incubation with Iodixanol was similar to the release in the control cultures. In comparison, lower endothelin-1 levels were measured in the cultures incubated with Iomeprol at all 3 time points indicating a more significant cell trauma. Prostacyclin release - also an indicator of traumatization of endothelial cells - increased after addition of both contrast agents. The highest release was found after incubation with Iomeprol 350.
Franke
R P
RP
Zentralinstitut für Biomedizinische Technik, Abteilung Biomaterialien, Universität Ulm, Ulm, Germany.
Fuhrmann
R
R
Hiebl
B
B
Jung
F
F
D002478:Cells, Cultured; D003287:Contrast Media / Q000494:pharmacology*; D019332:Endothelin-1 / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism*; D006801:Humans; D007479:Iopamidol / Q000031:analogs & derivatives; D009569:Nitric Oxide / Q000378:metabolism*; D011446:Prospective Studies; D014283:Triiodobenzoic Acids; D014471:Umbilical Veins / Q000166:cytology* / Q000187:drug effects
D003287:Contrast Media; D019332:Endothelin-1; D014283:Triiodobenzoic Acids; C057937:iomeprol; D009569:Nitric Oxide; D011464:Epoprostenol; C044834:iodixanol; D007479:Iopamidol