Effect of O2 concentrations on Sulfolobus solfataricus P2.
Sulfolobus solfataricus P2 was grown aerobically at various O(2) concentrations. Based on growth parameters in microcosms, four types of behavior could be distinguished. At 35% O(2) (v/v; gas phase), the cultures did not grow, indicating a lethal dose of oxygen. For 26-32% O(2), the growth was significantly affected compared with the reference (21%), suggesting a moderate toxicity by O(2). For 16-24% O(2), standard growth was observed. For 1.5-15% O(2), growth was comparable with the reference, but the yield on O(2) indicated a more efficient use of oxygen. These results indicate that S. solfataricus P2 grows optimally in the range of 1.5-24% O(2), most likely by adjusting its energy-transducing machinery. To gain some insight into control of the respiratory system, transcriptomes of the strain cultivated at different O(2) concentrations, corresponding to each behavior (1.5%, 21% and 26%), were compared using a DNA microarray approach. It showed differential expression of several genes encoding terminal oxidases, indicating an adaptation of the strain's respiratory system in response to fluctuating oxygen concentrations.
Simon
Gwénola
G
Institut de Recherche pour le Développement, Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, UMR 180, Universités de Provence et de la Méditerranée, ESIL, 163 Avenue de Luminy, Marseille Cedex 09, France.
Walther
Jasper
J
Zabeti
Nathalie
N
Combet-Blanc
Yannick
Y
Auria
Richard
R
van der Oost
John
J
Casalot
Laurence
L
D000332:Aerobiosis; D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020411:Oligonucleotide Array Sequence Analysis; D010100:Oxygen / Q000378:metabolism* / Q000494:pharmacology*; D048229:Sulfolobus solfataricus / Q000187:drug effects* / Q000254:growth & development
D000890:Anti-Infective Agents; D010100:Oxygen