[Expression of survivin gene in a rat model of acute pancreatitis].
OBJECTIVE
To investigate the expression of survivin in acute pancreatitis in rats.


METHODS
Acute pancreatitis was induced in rats by retrograde injection of sodium taurocholate into the pancreaticobiliary duct. The expressions of survivin in the pancreatic tissues was detected by immunohistochemisty, Western blotting and RT-PCR, and the apoptotic ratio of the acinar cells was determined by TUNEL assay.


RESULTS
Survivin was not detected in the control group, and in rat models of acute pancreatitis, the expressions of survivin protein and mRNA increased but the apoptotic index of the acinar cells decreased gradually with the severity of inflammation.


CONCLUSIONS
Survivin is involved in the regulation of acinar cell apoptosis and also the necrosis of the apoptotic acinar cells through its anti-apoptosis activity to aggravate acute pancreatitis, suggesting its value as a promising marker in predicting the severity of acute pancreatitis.
Li
Zhen-Dong
ZD
Department of Hepatobiliary Surgery, First Affiliated Hospital of Ji'nan University, Guangzhou, China. victor7922@163.com
Ma
Qing-Yong
QY
Luo
Yu-Hong
YH
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015415:Biomarkers; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D010195:Pancreatitis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077022:Survivin; D013656:Taurocholic Acid
D015415:Biomarkers; C516989:Birc5 protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D000077022:Survivin; D013656:Taurocholic Acid