Applications of new sequencing technologies for transcriptome analysis.
Transcriptome analysis has been a key area of biological inquiry for decades. Over the years, research in the field has progressed from candidate gene-based detection of RNAs using Northern blotting to high-throughput expression profiling driven by the advent of microarrays. Next-generation sequencing technologies have revolutionized transcriptomics by providing opportunities for multidimensional examinations of cellular transcriptomes in which high-throughput expression data are obtained at a single-base resolution.
Morozova
Olena
O
BC Cancer Agency, Genome Sciences Center, Vancouver, BC V5Z 4S6, Canada. omorozova@bcgsc.ca
Hirst
Martin
M
Marra
Marco A
MA
D000818:Animals; D004247:DNA / Q000032:analysis; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D016678:Genome; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D022661:RNA, Untranslated / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
D022661:RNA, Untranslated; D004247:DNA