Crystallization and preliminary X-ray analysis of the small subunit (R2F) of native ribonucleotide reductase from Corynebacterium ammoniagenes.
Ribonucleotide reduction, the unique step in DNA-precursor biosynthesis, involves radical-dependent redox chemistry and diverse metallo-cofactors. The metallo-cofactor (R2F) encoded by the nrdF (nucleotide reduction) gene in Corynebacterium ammoniagenes ATCC 6872 was isolated after homologous expression and a new crystal form of ribonucleotide reductase R2F was obtained. R2F was crystallized at 277 K using the vapour-diffusion method with PEG as the precipitating agent. A data set was collected to 1.36 A resolution from a single crystal at 100 K using synchrotron radiation. The crystal belonged to space group C2, with unit-cell parameters a = 96.21, b = 87.68, c = 83.25 A, beta = 99.29 degrees. The crystal contained two molecules per asymmetric unit, with a Matthews coefficient (V(M)) of 2.69 A(3) Da(-1); the solvent content was estimated to be 54.3%. X-ray fluorescence spectroscopy and MAD diffraction data indicated the presence of manganese in the molecule and the absence of iron.
Ogata
Hideaki
H
Max-Planck-Institut für Bioanorganische Chemie, Mülheim an der Ruhr, Germany.
Stolle
Patrick
P
Stehr
Matthias
M
Auling
Georg
G
Lubitz
Wolfgang
W
D003352:Corynebacterium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D021122:Protein Subunits / Q000737:chemistry*; D012264:Ribonucleotide Reductases / Q000737:chemistry*
D021122:Protein Subunits; D012264:Ribonucleotide Reductases