Nucleic acid-induced aggregation and pyrene excimer formation.
Nucleic acid was found to induce the aggregation of the positively charged pyrene probe (compound 1); as a result, strong pyrene excimer emission was observed. The intensity of the excimer emission was dependent on the concentration of the pyrene probe and the oligonucleotide length, sequence, and concentration. These results suggest a new strategy for label-free nucleic acid-based biosensing applications.
Zhang
Ruixing
R
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Tang
Dan
D
Lu
Ping
P
Yang
Xiangyu
X
Liao
Dongli
D
Zhang
Yujing
Y
Zhang
Mingjun
M
Yu
Cong
C
Yam
Vivian W W
VW
D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011721:Pyrenes; C030984:pyrene