Genetic heterogeneity and recombination in canine noroviruses.
Alphatronlike (genogroup IV [GIV]) noroviruses (NoVs) have been recently identified in carnivores. By screening a collection of 183 fecal samples collected during 2007 from dogs with enteric signs, the overall NoV prevalence was found to be 2.2% (4/183). A unique strain, Bari/91/07/ITA, resembled GIV.2 NoVs in its ORF1 (polymerase complex), while it was genetically unrelated in its full-length ORF2 (capsid gene) to GIV animal and human NoVs (54.0 to 54.4% amino acid identity) and to any other NoV genogroup (<54.7% amino acid identity). It displayed the highest identity (58.1% amino acid identity) to unclassified human strain Chiba/040502/04/Jp. Interestingly, the very 5' end of ORF2 of the canine virus matched short noroviral sequences (88.9% nucleotide identity and 98.9% amino acid identity) identified from oysters in Japan, indicating that similar viruses may be common environmental contaminants.
Martella
Vito
V
Department of Veterinary Public Health, University of Bari, Valenzano, Bari, Italy. v.martella@veterinaria.uniba.it
Decaro
Nicola
N
Lorusso
Eleonora
E
Radogna
Arianna
A
Moschidou
Paschalina
P
Amorisco
Francesca
F
Lucente
Maria Stella
MS
Desario
Costantina
C
Mari
Viviana
V
Elia
Gabriella
G
Banyai
Krisztian
K
Carmichael
Leland Eugene
LE
Buonavoglia
Canio
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary; D036022:Capsid Proteins / Q000235:genetics; D004285:Dogs; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary / Q000821:virology; D018740:Genetic Heterogeneity; D006801:Humans; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D011995:Recombination, Genetic
D036022:Capsid Proteins