title: [Diagnostic role of BIOMED-2 multiplex polymerase chain reaction for antigen receptor genes rearrangement in lymphoproliferative disorders].
abstract: OBJECTIVE
  To establish a sensitive and effective method for detection of immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangement,and to explore its role in diagnosis and differential diagnosis of lymphoproliferative disorders.
  
  
  METHODS
  Fifty-eight lymphoid tissue samples from 54 patients with lymphoproliferations were evaluated by the novel BIOMED-2 multiplex polymerase chain reaction (PCR) for antigen receptor genes rearrangement.
  
  
  RESULTS
  Multiplex PCR demonstrated monoclonal Ig/TCR gene rearrangements in 22 of 25 (88.0%) B-cell malignancies and 8 of 15 (53.3%) T-cell malignancies. Among 17 benign lymphoproliferations confirmed histopathologically, polyclonal rearrangements were detected in 14 cases (82.4%). In total, the clonality analysis and the final clinico-histopathological diagnosis were concordant in 77.2%. Combination detection of Iglambda and TCR delta gene rearrangements did not increase the detection rate of monoclonal rearrangement of Ig/TCR, but might help to the detection of Iglambda+ or TCR delta+ lymphomas.
  
  
  CONCLUSIONS
  The novel BIOMED-2 multiplex PCR strategy is a rapid, reliable and sensitive approach to detecting clonality in suspected lymphoproliferations, especially in atypical cases.
authors.affiliation: Department of Hematology, Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Huang
authors.forename: Deng-Ju
authors.initials: DJ
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.forename: Fan-Kai
authors.initials: FK
authors.lastname: Meng
authors.forename: Jin-Niu
authors.initials: JN
authors.lastname: Deng
authors.forename: Han-Ying
authors.initials: HY
authors.lastname: Sun
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Liu
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhou
mesh_terms: D005260:Female; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity