title: Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin.
abstract: A new fluorogenic, lanthanide-based oligopeptide substrate for the detection of the zinc-dependent endoprotease thermolysin is described. Using time-resolved fluorescence measurement, a highly sensitive assay for thermolysin was developed with a 50pM detection limit (3.5fmol).
authors.affiliation: Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
authors.forename: Ann-Kathrin
authors.initials: AK
authors.lastname: Marguerre
authors.forename: Roland
authors.initials: R
authors.lastname: Krämer
mesh_terms: D000595:Amino Acid Sequence; D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013820:Thermolysin / Q000032:analysis*; D015032:Zinc / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D005063:Europium; D013820:Thermolysin; D015032:Zinc