Interfacial behavior of a hairpin DNA probe immobilized on gold surfaces.
Neutron reflection has been used in combination with electrochemical and quartz microbalance techniques to characterize a mixed monolayer of thiolated hairpin ss-DNA and 4-mercaptobutan-1-ol monolayer self-assembled on gold before and after hybridization with a cDNA target. Neutron reflection has revealed the opening of the stem-loop configuration of the probe associated with helix formation. This change in conformation correlates to the modification of the electron-transfer resistance associated with the [Fe(CN)6](3-/4-) redox marker present in solution.
Steichen
M
M
Service de Chimie Analytique et Chimie des Interfaces, Faculté des Sciences, Université Libre de Bruxelles, Boulevard du Triomphe, CP 255, 1050 Bruxelles, Belgique.
Brouette
N
N
Buess-Herman
C
C
Fragneto
G
G
Sferrazza
M
M
D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D017097:Electric Impedance; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D055029:Inverted Repeat Sequences; D008958:Models, Molecular; D033363:Neutron Diffraction; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009693:Nucleic Acid Hybridization; D011791:Quartz / Q000737:chemistry; D013499:Surface Properties
D015342:DNA Probes; D011791:Quartz; D006046:Gold