title: Partial hindlimb occlusion reduced the susceptibility to sustained ventricular tachycardia in conscious rats.
abstract: Remote conditioning induced by ischemia in distant organs protects the heart from ischemia/reperfusion injury; however, its effect on ischemia-induced ventricular arrhythmias is unknown. Therefore, we tested the hypothesis that partial hindlimb occlusion during coronary artery occlusion increases the ventricular arrhythmia threshold (VAT) induced by coronary artery occlusion. Rats (n = 7) were instrumented with a radio-telemetry device for recording arterial pressure, electrocardiogram (ECG), and body temperature. A Doppler ultrasonic flow probe and vascular occluder were placed around the terminal aorta. Finally, a snare was placed around the left main coronary artery. The VAT was determined without and, on an alternate day, during partial hindlimb occlusion (remote conditioning) in conscious rats. Without remote conditioning, the VAT was 4.56 + 0.15 minutes. Importantly, remote conditioning significantly increased the VAT (6.29 + 0.49 minutes), suggesting that ischemia in a distant organ may delay the development of ischemia-induced ventricular arrhythmias.
authors.affiliation: Department of Physiology, Wayne State University, Detroit, Michigan 48201, USA. hlujan@med.wayne.edu
authors.forename: Heidi L
authors.initials: HL
authors.lastname: Lujan
authors.forename: Stephen E
authors.initials: SE
authors.lastname: DiCarlo
mesh_terms: D000818:Animals; D001011:Aorta / Q000503:physiopathology; D001783:Blood Flow Velocity; D001794:Blood Pressure; D018660:Blood Pressure Monitoring, Ambulatory; D001831:Body Temperature; D004195:Disease Models, Animal; D015716:Electrocardiography, Ambulatory; D006339:Heart Rate; D006614:Hindlimb; D019194:Ischemic Preconditioning / Q000379:methods*; D017078:Laser-Doppler Flowmetry; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply*; D017202:Myocardial Ischemia / Q000150:complications / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D017180:Tachycardia, Ventricular / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D013686:Telemetry; D013997:Time Factors