title: Differentiation-associated alteration in sensitivity to apoptosis induced by (-)-epigallocatechin-3-O-gallate in HL-60 cells.
abstract: Green tea and its constituent (-)-epigallocatechin-3-O-gallate (EGCG) are known to have apoptosis-inducing activity on tumor cells including human leukemia HL-60 cells, providing an explanation for their anti-cancer effects. In the present study, we compared the sensitivity of undifferentiated cells and differentiated HL-60 cells with normal-like phenotypic characters. HL-60 cells treated with three differentiating agents were found to be resistant to EGCG-mediated apoptosis as compared with undifferentiated cells. Gene and protein expression of 67 kDa laminin receptor was down-regulated in differentiated HL-60 cells, suggesting its contribution to the difference in sensitivity in view of the fact that the receptor is a target of EGCG's action to induce apoptosis. The finding supports the view that EGCG induces apoptosis preferentially in cancer cells as compared with normal counterparts.
authors.affiliation: University of Shizuoka, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Okada
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tanabe
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tazoe
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ishigami
authors.forename: Ryuuta
authors.initials: R
authors.lastname: Fukutomi
authors.forename: Kensuke
authors.initials: K
authors.lastname: Yasui
authors.forename: Mamoru
authors.initials: M
authors.lastname: Isemura
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D017474:Receptors, Laminin / Q000096:biosynthesis; D013662:Tea
chemical_list: D000975:Antioxidants; D017474:Receptors, Laminin; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate