title: LC determination of albiflorin and paeoniflorin in Bai Shao (Paeonia lactiflora) as a raw herb and dried aqueous extract.
abstract: A validated analytical method is reported for the analysis of paeoniflorin and albiflorin in Bai Shao (Paeonia lactiflora) as a dried raw herb and commercially prepared dried aqueous extract. The samples were extracted by sonication in methanol and the extract analyzed by LC-photodiode array with identity confirmation by electrospray ionization-tandem MS. A C18 column was used with an acetonitrile-water gradient mobile phase. Paeoniflorin and albiflorin were quantified at 230 nm. Ions with m/z 121 and 327 were produced with the MS detector, using the paeoniflorin precursor ion with m/z 479. For albiflorin, the precursor ion with m/z 479 produced the m/z 121 and 77 ions. The amounts of paeoniflorin and albiflorin found in the raw herb were 33.2 and 1.8 mg/g, respectively; and in the dried aqueous extract, the amounts were 34.8 and 15.7 mg/g, respectively. The LODs for paeoniflorin and albiflorin were 0.37 and 1.39 mg/g, respectively, for the raw herb and 0.25 and 0.06 mg/g, respectively, for the dried aqueous extract.
authors.affiliation: University of Western Sydney, Herbal Analysis and Pharmacological Laboratories, Centre for Complementary Medicine Research, Penrith South DC, New South Wales, 1797, Australia. s.lee@uws.edu.au
authors.forename: Samiuela
authors.initials: S
authors.lastname: Lee
authors.forename: Cheang S
authors.initials: CS
authors.lastname: Khoo
authors.forename: James R
authors.initials: JR
authors.lastname: Hennell
authors.forename: Jarryd L
authors.initials: JL
authors.lastname: Pearson
authors.forename: Mariam
authors.initials: M
authors.lastname: Jarouche
authors.forename: Clynton W
authors.initials: CW
authors.lastname: Halstead
authors.forename: Alan
authors.initials: A
authors.lastname: Bensoussan
mesh_terms: D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D005960:Glucosides / Q000032:analysis*; D007202:Indicators and Reagents; D008516:Medicine, Chinese Traditional; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D028321:Plant Preparations / Q000032:analysis; D012015:Reference Standards; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D007202:Indicators and Reagents; D039821:Monoterpenes; D010936:Plant Extracts; D028321:Plant Preparations; D012997:Solvents; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin