Comparison of gene expression profiles in a porcine infarct model after intracoronary, transthoracic, or transendocardiac injection of heterologous bone marrow mesenchymal cells.
An in vivo porcine model of myocardial infarction was developed with the aim of comparing the effectiveness for cardiac repair of intracoronary, transthoracic, or transendocardial delivery strategies for bone marrow mesenchymal stem cells (BMMSC) using an analysis of expression levels of transcripts related to various cellular processes at 8 heart regions using quantitative reverse transcriptase polymerase chain reaction. We observed significant rises in cardiomyogenic markers Mef2C, Gata4 and Nkx2.5, and contractibility marker Serca2A at infarcted regions for cell-treated pigs. We also observed differences in Sdf1 expression related to the organ stress response between delivery strategies. Unexpectedly, increased expression of Col1A1 was detected in 2 cell-treated groups at various heart regions. Our results suggest improvements in both contractility and cardiomyogenic capability of damaged tissue after BMMSC injection, but also warned us about the relevance of the chosen delivery strategy and potential undesired effects like increasing fibrosis after treatment.
Barallobre-Barreiro
J
J
Unidad de Investigación, INIBIC, CHU A Coruña, A Coruña, Spain.
de Ilárduya
O M
OM
Moscoso
I
I
Calviño
R
R
Aldama
G
G
López-Peláez
E
E
Centeno
A
A
Doménech
N
N
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods* / Q000662:veterinary; D018398:Homeodomain Proteins / Q000235:genetics; D026161:MADS Domain Proteins / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods* / Q000662:veterinary; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics; D013552:Swine; D014157:Transcription Factors / Q000235:genetics
D018398:Homeodomain Proteins; D026161:MADS Domain Proteins; D014157:Transcription Factors; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases