Immunosensors for detection of Annexin II and MUC5AC for early diagnosis of lung cancer.
Amperometric immunosensors were developed to diagnose lung cancer through the detection of Annexin II and MUC5AC. To fabricate the sensor probe, a conducting polymer (poly-terthiophene carboxylic acid; poly-TTCA) was electropolymerized onto a gold nanoparticle/glassy carbon electrode (AuNP/GCE) and a dendrimer (Den) was covalently bonded to the poly-TTCA through amide bond formation, where AuNPs were doped onto the dendrimer. To obtain the final sensor probe, an antibody (anti-Annexin II) and hydrazine (Hyd), which is a catalyst for the reduction of H(2)O(2) generated by glucose oxidase (GOx), were covalently attached onto the Den/AuNP-modified surface. Each surface was then characterized by SEM, impedance spectroscopy and XPS. The final sensor probe was examined before and after interaction with Annexin II and MUC5AC using impedance-spectroscopic, quartz crystal microbalance and amperometric methods. The performance of the immunosensor for the Annexin II was evaluated for the apical surface fluid labeled with GOx by the standard addition method. In this case, the detection limit of the proposed method was 0.051 ng/mL (k=3, n=5). The Annexin II concentration in the secretions collected from squamous metaplastic cells was determined to be 280+/-8.0 pg/mL (n=5).
Kim
Dong-Min
DM
Department of Chemistry and Institute BioPhysio Sensor Technology, Pusan National University, Busan 609-735, Republic of Korea.
Noh
Hui-Bog
HB
Park
Deog Su
DS
Ryu
Seung-Hee
SH
Koo
Ja Seok
JS
Shim
Yoon-Bo
YB
D017306:Annexin A2 / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D055271:Mucin 5AC / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers
C472822:ANXA2 protein, human; D017306:Annexin A2; D014408:Biomarkers, Tumor; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D009363:Neoplasm Proteins