title: Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis.
abstract: OsDREB1D, a special DREB (dehydration responsive element binding protein) homologous gene, whose transcripts cannot be detected in rice (Oryza sativa L), either with or without stress treatments, was amplified from the rice genome DNA. The yeast one-hybrid assay revealed that OsDREB1D was able to form a complex with the dehydration responsive element/C-repeat motif. It can also bind with a sequence of LTRE (low temperature responsive element). To analyze the function of OsDREB1D, the gene was transformed and over-expressed in Arabidopsis thaliana cv. Columbia. Results indicated that the over-expression of OsDREB1D conferred cold and high-salt tolerance in transgenic plants, and that transgenic plants were also insensitive to ABA (abscisic acid). From these data, we deduced that this OsDREB1D gene functions similarly as other DREB transcription factors. The expression of OsDREB1D in rice may be controlled by a special mechanism for the redundancy of function.
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Xiao-Fen
authors.initials: XF
authors.lastname: Jin
authors.forename: Ai-Sheng
authors.initials: AS
authors.lastname: Xiong
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.forename: Yi-Huan
authors.initials: YH
authors.lastname: Hong
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D003080:Cold Temperature; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding / Q000187:drug effects; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D000040:Abscisic Acid