Trypsin-gold nanoparticle conjugates: binding, enzymatic activity, and stability.
Nanomaterials may interact with biomolecules in various ways and change their bioactivities. Here, we report on how gold nanoparticles (AuNPs) affect a most important protease, trypsin. After simple mixing of the trypsin and AuNPs solution the average diameter is 20 nm under enzyme friendly conditions (pH 8.0); the bond between trypsin and AuNPs was detected by UV-vis spectroscopy. The further protease assay of trypsin, before and after mixing with the AuNPs solution, pointed out an improved performance in terms of enzyme activity and stability.
Lv
Min
M
School of Life Sciences, Sichuan University, Sichuan, P. R. China.
Zhu
Engao
E
Su
Yuanyuan
Y
Li
Qingnuan
Q
Li
Wenxin
W
Zhao
Yun
Y
Huang
Qing
Q
D001665:Binding Sites; D004789:Enzyme Activation; D004795:Enzyme Stability; D006046:Gold / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011485:Protein Binding; D014357:Trypsin / Q000737:chemistry* / Q000648:ultrastructure*
D006046:Gold; D014357:Trypsin