Remodeling of the tight junction during recovery from exposure to hydrogen peroxide in kidney epithelial cells.
Renal ischemia-reperfusion injury results in oxidative stress-induced alterations in barrier function. Activation of the mitogen-activated protein (MAP) kinase pathway during recovery from oxidative stress may be an effector of oxidant-induced tight junction reorganization. We hypothesized that tight junction composition and barrier function would be perturbed during recovery from oxidative stress. We developed a model of short-term H(2)O(2) exposure followed by recovery using Madin Darby canine kidney (MDCK II) cells. H(2)O(2) perturbs barrier function without a significant cytotoxic effect except in significant doses. ERK-1/2 and p38, both enzymes of the MAP kinase pathway, were activated within minutes of exposure to H(2)O(2). Transient exposure to H(2)O(2) produced a biphasic response in the transepithelial electrical resistance (TER). An initial drop in TER at 6 h was followed by a significant increase at 24 h. Inhibition of ERK-1/2 activation attenuated the increase in TER observed at 24 h. Expression of occludin initially decreased, followed by partial recovery at 24 h. In contrast, claudin-1 levels decreased and failed to recover at 24 h. Claudin-2 levels were markedly decreased at 24 h; however, inhibition of ERK-1/2 activation was protective. Occludin and claudin-1 localization at the apical membrane on immunofluorescence images was fragmented at 6 h after H(2)O(2) exposure with subsequent recovery of appropriate localization by 24 h. MDCK II cell recovery after H(2)O(2) exposure is associated with functional and structural modifications of the tight junction that are mediated in part by activation of the MAP kinase enzymes ERK-1/2 and p38.
Gonzalez
Jeannette E
JE
Wilford Hall Medical Center, Lackland AFB, TX 78236, USA.
DiGeronimo
Robert J
RJ
Arthur
D'Ann E
DE
King
Jonathan M
JM
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D057167:Claudins / Q000096:biosynthesis; D004285:Dogs; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008565:Membrane Proteins / Q000096:biosynthesis; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D062793:Occludin; D018384:Oxidative Stress; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D057167:Claudins; D008565:Membrane Proteins; D062793:Occludin; D006861:Hydrogen Peroxide; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases