title: Phytase gene expression in Lactobacillus and analysis of its biochemical characteristics.
abstract: A 1.4-kb DNA containing the coding region of phytase gene from Aspergillus ficuum (A. ficuum) was connected with the plasmid of pIAbeta8 to construct shuffling vector, which was inserted into Lactobacillus casei (L. casei) by electroporation. The Lactobacillus with phytase gene was selected and incubated in anaerobic liquid medium. The results indicated that the highest phytase activities in the supernatant and cells were 22.12 and 4.49 Uml(-1) (P<0.05) at the fourth day of incubation; the optimum pH and temperature of phytase were 5.0 and 40-80 degrees C, respectively (P<0.05); the lowest pH value in the anaerobic medium was 3.35 (P<0.05) at the third day incubation; and the molecular weight of the expressed phytase was 39.2 kDa.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China.
authors.forename: Ruiyu
authors.initials: R
authors.lastname: Zuo
authors.forename: Juan
authors.initials: J
authors.lastname: Chang
authors.forename: Qingqiang
authors.initials: Q
authors.lastname: Yin
authors.forename: Liying
authors.initials: L
authors.lastname: Chen
authors.forename: Qixin
authors.initials: Q
authors.lastname: Chen
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Zheng
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Ren
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
mesh_terms: D010832:6-Phytase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000201:enzymology; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007780:Lacticaseibacillus casei / Q000235:genetics*; D013696:Temperature
chemical_list: D010832:6-Phytase