Cellulose hydrolysis by immobilized Trichoderma reesei cellulase.
Cellulose hydrolysis by immobilized Trichoderma reesei cellulase in the presence of a low viscosity ionic liquid, 1-ethyl-3-methylimidazolium diethyl phosphate (EMIM-DEP), was investigated. Preparation of the carrier-free immobilized cellulase was optimized with respect to concentration of the cross-linker and the type of precipitant. The addition of 2% (v/v) EMIM-DEP during hydrolysis gave an initial reaction rate 2.7 times higher than the hydrolysis rate with no ionic liquid. The initial yield after 2 h was 0.7 g glucose/g cellulose, and the carrier-free immobilized cellulase (CFIC) was effectively re-used five times.
Jones
Paetrice O
PO
Department of Chemical Engineering, Kingsbury Hall, University of New Hampshire, Durham, NH 03824, USA.
Vasudevan
Palligarnai T
PT
D002480:Cellulase / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology*
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D007093:Imidazoles; D052578:Ionic Liquids; D002482:Cellulose; D002480:Cellulase; C518739:1-ethyl-3-methylimidazolium