Hematopoietic capacity of preterm cord blood hematopoietic stem/progenitor cells.
Full-term cord blood (TCB) hematopoietic stem/progenitor cells (HSC/HPCs) are used for stem cell transplantation and are well characterized. However, the properties of preterm cord blood (PCB) HSC/HPCs remain unclear. In the present study, we compared HSC/HPCs from TCB and PCB with respect to their expression of surface markers, homing capacity and ability to repopulate HSCs in the NOD/Shi-scid mice bone marrow. The proportion of CD34+CD38- cells was significantly higher in PCB. On the other hand, the engraftment rate of TCB CD34+ cells into NOD/Shi-scid mice was significantly higher than PCB CD34+ cells. The expression of VLA4 was stronger among TCB CD34+ cells than PCB CD34+ cells. Moreover, there was a positive correlation between the proportion of CD34+CXCR4+ cells and gestational age. These data suggest that the homing ability of HSCs increases during gestation, so that TCB may be a better source of HSCs for transplantation than PCB.
Nakajima
Mizue
M
Department of Pediatrics, Nippon Medical School, Tokyo 113-8602, Japan.
Ueda
Takahiro
T
Migita
Makoto
M
Oue
Yuki
Y
Shima
Yoshio
Y
Shimada
Takashi
T
Fukunaga
Yoshitaka
Y
D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis; D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D002465:Cell Movement; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D039041:Integrin alpha4beta1 / Q000096:biosynthesis; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011247:Pregnancy
D018952:Antigens, CD34; D039041:Integrin alpha4beta1; D051997:ADP-ribosyl Cyclase 1