title: Correlation of gingival crevicular fluid interleukin-18 and monocyte chemoattractant protein-1 levels in periodontal health and disease.
abstract: BACKGROUND
  Interleukin (IL)-18 is a proinflammatory cytokine of the IL-1 superfamily and is unique, with the capacity to induce T-helper (Th)1 or Th2 differentiation depending on the immunologic context. Monocyte chemoattractant protein (MCP)-1 is a CC chemokine responsible for chemotaxis of monocytes. A previous study showed the induction of MCP-1 by IL-18 in mice macrophages. The present study was carried out to examine the gingival crevicular fluid (GCF) levels of IL-18 and MCP-1 in periodontal health and disease and to evaluate any correlation between IL-18 and MCP-1 GCF levels.
  
  
  METHODS
  Sixty subjects (30 males and 30 females; age range: 26 to 49 years) participated in the study. The subjects were initially divided into three groups, consisting of 20 subjects in each group, based on the gingival index, probing depth (PD), clinical attachment loss (AL), and radiologic parameters (bone loss): healthy (group 1), gingivitis (group 2), and periodontitis (group 3), whereas patients in group 3 after treatment constituted group 4. GCF samples were collected from all of the groups to estimate the levels of IL-18 and MCP-1 using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The mean IL-18 and MCP-1 concentrations in GCF were highest in group 3 (330.61 pg/microl and 73.3 pg/microl, respectively). The results suggest that IL-18 and MCP-1 levels increased in GCF from periodontal health to disease and decreased after periodontal therapy. Levels of IL-18 and MCP-1 positively correlated with PD and clinical AL in group 3. In addition, IL-18 and MCP-1 levels significantly correlated with each other in groups 2, 3, and 4.
  
  
  CONCLUSIONS
  GCF IL-18 and MCP-1 concentrations increased in periodontal disease compared to health and correlated positively with the severity of disease. Further, based on the positive correlation of IL-18 and MCP-1 found in this study, it can be proposed that IL-18 may promote an inflammatory response by the induction of MCP-1 production and the subsequent recruitment and activation of circulating leukocytes at the inflammatory site.
authors.affiliation: Department of Periodontics, Government Dental College and Research Institute, Fort, Bangalore, Karnataka, India. periodontics_gdc@yahoo.co.in
authors.forename: A R
authors.initials: AR
authors.lastname: Pradeep
authors.forename: Happy
authors.initials: H
authors.lastname: Daisy
authors.forename: Parag
authors.initials: P
authors.lastname: Hadge
authors.forename: Garima
authors.initials: G
authors.lastname: Garg
authors.forename: Manojkumar
authors.initials: M
authors.lastname: Thorat
mesh_terms: D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000276:immunology / Q000628:therapy; D015415:Biomarkers / Q000032:analysis; D018932:Chemokine CCL2 / Q000032:analysis*; D055113:Chronic Periodontitis / Q000276:immunology / Q000628:therapy; D003773:Dental Plaque / Q000628:therapy; D012534:Dental Scaling; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000276:immunology*; D005891:Gingivitis / Q000276:immunology / Q000628:therapy; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D020382:Interleukin-18 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009910:Oral Hygiene; D017622:Periodontal Attachment Loss / Q000276:immunology / Q000628:therapy; D010510:Periodontal Diseases / Q000276:immunology* / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000276:immunology / Q000628:therapy; D010519:Periodontium / Q000276:immunology*; D016745:Root Planing
chemical_list: D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D018836:Inflammation Mediators; D020382:Interleukin-18