title: [Activation of cloned BKCa channels in Ang II-induced proliferation of HEK293 cells].
abstract: OBJECTIVE
  To investigate the mediating and regulating role of BK(Ca) channels in Ang II-induced cell proliferation.
  
  
  METHODS
  Using Lipofectamine 2000, the pIRES-hSloalpha plasmid was transfected into HEK293 cells. The concentration-dependent cuve of Ang II-induced cell proliferation was tested by MTT colorimetry. The effect of IbTX, Ang II, Ang II + IbTX on the proliferating cell nuclear antigen (PCNA) expression and cell cycle of transfected HEK293-hSloalpha cells were detected by immunocytochemistry and flow cytometry, respectively.
  
  
  RESULTS
  Ang II induced proliferation of transfected HEK293-hSloalpha cells in a concentration-dependent manner. PCNA expression of transfected HEK293-hSloalpha cells was enhanced by Ang II, and the percentage of S phase HEK293-hSloalpha cells was increased after Ang II treatment. However this effect was inhibited by IbTX, a selective BK(Ca) channel blocker.
  
  
  CONCLUSIONS
  BK(Ca) channels play an potential role in mediating Ang II-induced proliferation of HEK293-hSloalpha cells.
authors.affiliation: Department of Aerospace Physiology, Key Laboratory of Aerospace Medicine of Ministry of Education, Fourth Military Medical University, Xi'an 710032, China. lijun.aerospace@163.com
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Chang-lei
authors.initials: CL
authors.lastname: Deng
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Yu
authors.forename: Yao-ming
authors.initials: YM
authors.lastname: Chang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Man-jiang
authors.initials: MJ
authors.lastname: Xie
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000235:genetics / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D014162:Transfection
chemical_list: D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; D000804:Angiotensin II; C064719:iberiotoxin