title: Proteomic characterization of midproliferative and midsecretory human endometrium.
abstract: This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIalpha, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.
authors.affiliation: Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Victoria 3168, Australia. jenny.chen@princehenrys.org
authors.forename: Jenny I C
authors.initials: JI
authors.lastname: Chen
authors.forename: Natalie J
authors.initials: NJ
authors.lastname: Hannan
authors.forename: Yunxian
authors.initials: Y
authors.lastname: Mak
authors.forename: Peter K
authors.initials: PK
authors.lastname: Nicholls
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Adam
authors.initials: A
authors.lastname: Rainczuk
authors.forename: Peter G
authors.initials: PG
authors.lastname: Stanton
authors.forename: David M
authors.initials: DM
authors.lastname: Robertson
authors.forename: Lois A
authors.initials: LA
authors.lastname: Salamonsen
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Stephens
mesh_terms: D049109:Cell Proliferation; D015180:Electrophoresis, Gel, Two-Dimensional; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome