Over-expression of ThpI from Choristoneura fumiferana enhances tolerance to cold in Arabidopsis.
Thermal hysteresis proteins (Thps) known as antifreeze proteins for their antifreeze activity, depress the freezing point of water below the melting point in many polar marine fishes, terrestrial arthropods and plants. For the purpose of breeding cold-resistant plants, we designed to introduce the Thp gene into the plants. The physiological and biochemical effect of high-lever expression of the modified Choristoneura fumiferana Thp (ThpI) in Arabidopsis thaliana plants was analyzed. Under low temperature stress, the ThpI transgenic plants exhibited stronger growth than wild-type plants. The elevated cold tolerance of the ThpI over-expressing plants was confirmed by the changes of electrolyte leakage activity, malonyldialdehyde and proline contents. These results preliminarily showed that the Thp possibly be used to enhance the low temperature-tolerant ability of plants.
Zhu
Bo
B
Agro-Biotechnology Research Center, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, 201106 Shanghai, China.
Xiong
Ai-Sheng
AS
Peng
Ri-He
RH
Xu
Jing
J
Jin
Xiao-Fen
XF
Meng
Xiu-Rong
XR
Yao
Quan-Hong
QH
D000064:Acclimatization / Q000235:genetics*; D000818:Animals; D021301:Antifreeze Proteins / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D007915:Lepidoptera / Q000235:genetics*; D008315:Malondialdehyde / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation; D014882:Water-Electrolyte Balance / Q000235:genetics
D021301:Antifreeze Proteins; D008315:Malondialdehyde; D011392:Proline