Up-regulation in the expression of renin gene by the influence of aluminium.
The excretion of aluminium in urine was significantly increased after intake of analgesics containing aluminium, confirming increased absorption and hence exposure to aluminium with such medication. The effect of aluminium on the kidney was further investigated by study of gene expression in mice. After a single dose of aluminium, an up-regulation of renin gene was found by DNA sequencing of the products of differential display analysis. The up-regulation of renin was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting experiments in the dose dependent treatments and the time course observation after aluminium citrate injection. The up-regulation of the renin expression by aluminium is a strong indication of the influence of aluminium on the renin-angiotensin-aldosterone-system, resulting in possible induction of essential hypertension.
Ezomo
Ojeiru F
OF
Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-machi, Yonago 683-8503, Japan.
Matsushima
Fumiko
F
Meshitsuka
Shunsuke
S
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000187:drug effects / Q000378:metabolism; D000535:Aluminum / Q000494:pharmacology* / Q000652:urine; D000818:Animals; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D000071518:Liver X Receptors; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D057093:Orphan Nuclear Receptors / Q000187:drug effects / Q000378:metabolism; D012083:Renin / Q000096:biosynthesis* / Q000235:genetics; D012084:Renin-Angiotensin System / Q000187:drug effects; D015854:Up-Regulation; D055815:Young Adult
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D000535:Aluminum; D012083:Renin