title: Specifically binding of L-ficolin to N-glycans of HCV envelope glycoproteins E1 and E2 leads to complement activation.
abstract: L-ficolin, one of lectin families, is a recently identified complement factor that initiates lectin pathway of complement. Little is known about its role in viral hepatitis. In the present study, we found that L-ficolin in serum from 103 patients with hepatitis C virus (HCV), were significantly higher than that in 150 healthy controls. We further found that L-ficolin expressions were significantly increased in vitro study by HCV JFH-1 infected human hepatocyte cell line Huh7.5.1. Investigation of the mechanisms of the L-ficolin action on HCV demonstrated that L-ficolin protein could recognize and bind to envelope glycoproteins E1 and E2 of HCV, activating the lectin complement pathway-mediated cytolytic activity in HCV-infected hepatocyte. This interaction between L-ficolin and HCV E1 and E2 glycoproteins was attributed to the N-glycans of E1 and E2. These findings provide new insights into the biological functions of L-ficolin in clinically important hepatic viral diseases.
authors.affiliation: The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan 430071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Mohammed A M
authors.initials: MA
authors.lastname: Ali
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Shi
authors.forename: Yinglan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Fenglin
authors.initials: F
authors.lastname: Luo
authors.forename: Jin
authors.initials: J
authors.lastname: Yu
authors.forename: Tian
authors.initials: T
authors.lastname: Xiang
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Dongqing
authors.initials: D
authors.lastname: Li
authors.forename: Quan
authors.initials: Q
authors.lastname: Hu
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Ho
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D003167:Complement Activation / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D016174:Hepacivirus / Q000276:immunology* / Q000472:pathogenicity; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D022781:Hepatocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D006801:Humans; D037102:Lectins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D014759:Viral Envelope Proteins / Q000378:metabolism; D000097604:Ficolins
chemical_list: D037102:Lectins; D011134:Polysaccharides; D014759:Viral Envelope Proteins; D000097604:Ficolins; C087035:E1 protein, Hepatitis C virus; C091015:glycoprotein E2, Hepatitis C virus