title: Plasma proteome profiling of von Hippel-Lindau disease after total and subtotal nephrectomy: a preliminary study.
abstract: OBJECTIVE
  Common treatment of renal cell carcinoma associated with von Hippel-Lindau (VHL) disease is total (bilateral) or subtotal nephrectomy. Whereas total nephrectomy is associated with absolutely no residual renal function, subtotal nephrectomy frequently leads to chronic kidney disease (CKD) with some residual renal functions. However, molecular mechanisms underlying CKD remain unclear and the diagnosis of CKD is frequently accomplished at its late stage.
  
  
  METHODS
  We performed a plasma proteomics study to compare the plasma proteome profile of VHL patient who underwent total nephrectomy to the profiles of VHL patient with subtotal nephrectomy and healthy control. Totally 100 mug proteins from each sample was resolved by two-dimensional electrophoresis (2-DE) in triplicate and visualized with SYPRO Ruby fluorescence stain.
  
  
  RESULTS
  The normal plasma proteome profile markedly differed from the profiles of VHL patients. Comparative analysis between total versus subtotal nephrectomized patients revealed significant differences in levels of 20 plasma proteins. Pathway analysis revealed two important networks involving in lipid metabolism, molecular transport, carbohydrate metabolism, cellular growth and proliferation, and small molecule biochemistry, in which these identified and other proteins interplayed.
  
  
  CONCLUSIONS
  Our data identified potential biomarkers for CKD. Further characterization of these identified proteins might also lead to better understanding of molecular mechanisms underlying CKD.
authors.affiliation: Department of Internal Medicine I, Charles University School of Medicine in Plzen, Plzen, Czech Republic.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Opatrná
authors.forename: Wararat
authors.initials: W
authors.lastname: Chiangjong
authors.forename: Marie
authors.initials: M
authors.lastname: Korabecná
authors.forename: Kitisak
authors.initials: K
authors.lastname: Sintiprungrat
authors.forename: Supachok
authors.initials: S
authors.lastname: Sinchaikul
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
authors.forename: Martin
authors.initials: M
authors.lastname: Matejovic
authors.forename: Visith
authors.initials: V
authors.lastname: Thongboonkerd
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000473:pathology / Q000601:surgery; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D007680:Kidney Neoplasms / Q000097:blood / Q000473:pathology / Q000601:surgery; D008297:Male; D009392:Nephrectomy; D009942:Organometallic Compounds / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D006623:von Hippel-Lindau Disease / Q000097:blood / Q000473:pathology / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D009942:Organometallic Compounds; D020543:Proteome; C515601:Sypro Ruby