[Studies on purification of total saponins from the leaves of Acanthopanax trifoliatus by macroporous adsorption resin].
OBJECTIVE
To study the enrichment and purification of total saponins from the leaves of A. trifoliatus.


METHODS
Using the total saponins from the leaves of A. trifoliantus as the standard index, we examinad the capability of static adsorption and desorption to select the optimum macroporous adsportion resin. Then we studied the purification technological parameters such as pH value, the dynamic adorption speed of flow, the best type quantity, the eluent density and so on.


RESULTS
The optimum purification conditions were as follows: the type of macroporous adsorption resin was AB-8, the pH value of absorbing solution was 5-6, the absorbing velocity of flow was 1.0 mL/min, the concentration of ethanol as elutent was 70%, the proportion of the amount of absorbing solution and resins was 4:1, the proportion of the amount of elutent and resins was 4:1, the eluting velocity of flow was 2.0 mL/min.
Gao
Xia
X
College of Life Science/Key Laboratory of Environmental Science and Biodiversity Conservation of Sichuan Higher Education, China West Normal University, Nanchong 637002, China.
Li
Yun-Xiang
YX
Cai
Ling-Yun
LY
Wang
Rong
R
Quan
Qiu-Mei
QM
Chen
Jiao
J
D000327:Adsorption; D028001:Eleutherococcus / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*
D012117:Resins, Synthetic; D012503:Saponins; D000431:Ethanol