title: DcR3 protects THP-1 macrophages from apoptosis by increasing integrin alpha4.
abstract: In order to analyze the function of DcR3 for the regulation of cell adhesion and apoptosis in macrophages, we investigated the expression of decoy receptor 3 (DcR3) in THP-1 monocytes/macrophages. DcR3 was expressed in THP-1 and increased by phorbol 12-myristate 13-acetate (PMA). The formation of macrophage aggregates was observed when THP-1 cells were differentiated by PMA or stimulated with DcR3-Fc. Undifferentiated THP-1 cells were also induced to form aggregates by DcR3-Fc. The expression of integrin alpha4 was significantly increased by DcR3-Fc. CHX-induced apoptosis in THP-1 was inhibited by DcR3-Fc, of which inhibition against CHX-induced apoptosis and aggregate formation were ameliorated by anti-VLA4 antibody. DcR3 may play a significant role in macrophages not only by a decoy receptor but also by increasing alpha4 integrin.
authors.affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Tateishi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miura
authors.forename: Shinya
authors.initials: S
authors.lastname: Hayashi
authors.forename: Masayasu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kurosaka
mesh_terms: D000906:Antibodies / Q000276:immunology; D017209:Apoptosis; D002460:Cell Line; D006801:Humans; D039001:Integrin alpha Chains / Q000037:antagonists & inhibitors / Q000276:immunology; D039441:Integrin alpha4 / Q000096:biosynthesis*; D008264:Macrophages / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053323:Receptors, Tumor Necrosis Factor, Member 6b / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; C063493:CDw49d; D039001:Integrin alpha Chains; D012333:RNA, Messenger; D053323:Receptors, Tumor Necrosis Factor, Member 6b; C505911:TNFRSF6B protein, human; D039441:Integrin alpha4