Novel biological insights through metabolomics and 13C-flux analysis.
Metabolomics and (13)C-flux analysis have become instrumental for analyzing cellular metabolism and its regulation. Driven primarily by technical advances in mass spectrometry-based analytics, they provide unmatched readouts on metabolic state and activity. Functional genomics leverages metabolomics for the discovery of novel enzymes and unexpected secondary activities of annotated enzymes. (13)C-flux analyses are frequently used for empirical elucidation of pathways in poorly characterized species and for network-wide analysis of mechanisms that realize energy and redox balancing. Integration of metabolomics, (13)C-flux analysis and other data enable the condition-dependent characterization of regulatory circuits that ultimately govern the metabolic phenotype.
Zamboni
Nicola
N
Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland.
Sauer
Uwe
U
D002247:Carbon Isotopes / Q000032:analysis*; D023281:Genomics; D055432:Metabolomics / Q000379:methods*; D008954:Models, Biological
D002247:Carbon Isotopes