Anti-inflammatory and analgesic properties of cis-mulberroside A from Ramulus mori.
This study examined the analgesic and anti-inflammatory actions of cis-mulberroside A isolated from Ramulus mori in several models of inflammatory pain in mice. Cis-mulberroside A (25 and 50mg/kg) given by p.o. route 30 min before challenge produced a dose-dependent inhibition of the acetic acid-induced pain and Evans blue leakage in mice. In addition, this compound exhibited significant systemic anti-inflammatory activity in carrageenan-induced mouse paw edema in a concentration-related manner (33.1-68.5% inhibition), and similar results were achieved in formalin test. Suppressive effects of cis-mulberroside A on the production of NO and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated macrophages were also assessed. Collectively, cis-mulberroside A showed high analgesic and anti-inflammatory activities. The above results will be the supporting evidence for the potential anti-rheumatoid activity of R.mori in Chinese traditional medicine.
Zhang
Zuofa
Z
College of Animal Science, Zhejiang University, Hangzhou, China.
Shi
Liangen
L
D019342:Acetic Acid; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D002460:Cell Line; D004187:Disaccharides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D005070:Evans Blue; D005557:Formaldehyde; D007249:Inflammation / Q000188:drug therapy; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D029586:Moraceae / Q000737:chemistry*; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010146:Pain / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018547:Plant Stems; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D004187:Disaccharides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D013267:Stilbenes; C420606:mulberroside A; D005557:Formaldehyde; D009569:Nitric Oxide; D005070:Evans Blue; D002351:Carrageenan; D052247:Nitric Oxide Synthase Type II; D019342:Acetic Acid