title: [Spontaneous aneuploidy level in blood cells of fertile females].
abstract: In this study we present the results of molecular-cytogenetic investigation of blood cells of fertile women of reproductive age. We used cultivated and uncultivated cells and two sets of FISH probes with direct and indirect labeling. The mean aneuploidy levels of 4 chromosomes were determined and statistical limits were scored. Aneuploidy levels determined in cultivated lymphocytes by multicolor FISH varied from 0.1 to 1.3%. The mean aneuploidy level for all chromosomes (13, 18, 21 and X) was 1.39%. The limit mutation detection was 3.4%. We found differences in results obtained with the use of direct and indirect labeling. It was shown that cultivating process influenced the aneuploidy level. Absolute error of FISH technique was 0.13%, relative error - 4.08%.
authors.forename: N V
authors.initials: NV
authors.lastname: Zotova
authors.forename: E V
authors.initials: EV
authors.lastname: Markova
authors.forename: I N
authors.initials: IN
authors.lastname: Lebedev
authors.forename: A V
authors.initials: AV
authors.lastname: Svetliakov
mesh_terms: D000328:Adult; D000782:Aneuploidy; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007962:Leukocytes
chemical_list: D015342:DNA Probes