title: Protective effect of Antrodia camphorata on bladder ischemia/reperfusion injury.
abstract: BACKGROUND
  Obstructive bladder dysfunction is directly related to ischemia/reperfusion injury characterized by damage to nerves, synapses and smooth muscle cells within the bladder wall. Antrodia Camphorata (AC) has significant antioxidant, antiinflammatory and cell-cycle inhibition properties. The specific aim of this study was to evaluate whether orally administered AC can protect rabbit bladders from the progressive dysfunctions induced by bilateral ischemia/reperfusion (I/R).
  
  
  METHODS
  Twenty-four male NZW rabbits were separated into 4 groups of 6 animals each. Rabbits in groups 1 and 2 were fed Antrodia Camphorata (AC) suspensions; those in groups 3 and 4 received vehicle. Each rabbit in groups 2 and 4 were subjected to in vivo bilateral ischemia for 2 h and then allowed to recover for 1 week. The rabbits in groups 1 and 3 received sham operation and served as control groups. Cystometry, contractile responses to field stimulation, carbachol, ATP and KCl were determined. Biochemical and immuno-histochemical studies were also performed.
  
  
  RESULTS
  I/R resulted in decreased compliance, decreased contractile responses, decreased nerve density, and increased apoptosis. AC pretreatment of rabbits subjected to I/R significantly protected the bladder from all contractile, biochemical, and structural dysfunctions resulting in significantly improved bladder.
authors.affiliation: Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan.
authors.forename: Yung-Shun
authors.initials: YS
authors.lastname: Juan
authors.forename: Anita
authors.initials: A
authors.lastname: Mannikarottu
authors.forename: Shu Mien
authors.initials: SM
authors.lastname: Chuang
authors.forename: Suning
authors.initials: S
authors.lastname: Li
authors.forename: Alpha D
authors.initials: AD
authors.lastname: Lin
authors.forename: Lan
authors.initials: L
authors.lastname: Chang-Chou
authors.forename: Catherine
authors.initials: C
authors.lastname: Schuler
authors.forename: Robert E
authors.initials: RE
authors.lastname: Leggett
authors.forename: Robert M
authors.initials: RM
authors.lastname: Levin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D055491:Antrodia; D017209:Apoptosis; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000473:pathology / Q000503:physiopathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage*; D011817:Rabbits; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D001743:Urinary Bladder / Q000473:pathology / Q000503:physiopathology*
chemical_list: D010936:Plant Extracts