title: Minimum endotoxin concentration causing inflammation in the anterior segment of rabbit eyes.
abstract: OBJECTIVE
  This was a quantitative study to investigate the minimum endotoxin concentration causing inflammation in the anterior segment of the eye.
  
  
  METHODS
  Endotoxin was injected intracamerally in pigmented rabbits. A quantitative determination of flare and cells in the aqueous was performed using a laser flare-cell photometer, before and until 72 h after the treatment. An area under the curve (AUC) analysis was employed to evaluate the whole inflammatory reaction regarding flare values.
  
  
  RESULTS
  The time course of flare values in each endotoxin group showed a similar pattern with a peak value at 3 h. An AUC corresponding to values for "average +2sigma", 19301.8 in control eyes, was considered the cutoff value. Using this cutoff value and the regression curve in endotoxin-treated groups, the minimum endotoxin concentration causing inflammation regarding flare values was determined to be 0.60 endotoxin units (EU). Cell counts (cells/0.5 mm(3).0.5 s) corresponding to the value "average +2sigma", 6.07 at 24 h, in control eyes was considered to be the cutoff value. The minimum endotoxin concentration regarding cell counts was determined to be 0.23 EU.
  
  
  CONCLUSIONS
  There was a dissociation in response between flare and cells in the aqueous to intracameral endotoxin. The minimum endotoxin concentration causing inflammation ranged between 0.23 and 0.60 EU.
authors.affiliation: Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Sakimoto
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Sawa
authors.forename: Taiki
authors.initials: T
authors.lastname: Oshida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sugaya
authors.forename: Taisuke
authors.initials: T
authors.lastname: Hirono
authors.forename: Akiko
authors.initials: A
authors.lastname: Ishimori
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000187:drug effects* / Q000473:pathology; D019540:Area Under Curve; D018916:Blood-Aqueous Barrier / Q000187:drug effects*; D002452:Cell Count; D004731:Endotoxins / Q000008:administration & dosage* / Q000633:toxicity*; D015590:Fluorophotometry; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity; D011817:Rabbits; D014606:Uveitis, Anterior / Q000139:chemically induced* / Q000175:diagnosis
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; C044101:endotoxin, Escherichia coli