[Preparation and application of biotinylated anti-AIB1 moniclonal antibody].
OBJECTIVE
To cross-link the McAb 1A2E1 to BNHS reagents and apply this biotinylated McAb to detect AIB1 antigen of target cells.


METHODS
The McAb 1A2E1 was mixed with BNHS reagents to generate Biotin-1A2E1. The competitive inhibition ELISA and immunocytochemical method were applied to detect the biologic activity of antibody.


RESULTS
The antibody kept high biological activities (with a titer of 1 : 3200) and sensitivities in detecting the AIB1 protein of breast cancer cell.


CONCLUSIONS
The method of preparing biotinylated McAb is successful, and the prepared biotinylated McAb can be applied to detect target cells which express AIB1 antigen. This McAb provide useful tool for tumor auxiliary diagnosis.
Pang
Qiu-Xia
QX
Department of Biochemistry and Molecular Biology, Medical School of Yan'an University, Yan'an 716000, China.
Qu
Yi
Y
Wei
Da-Peng
DP
Zhao
Feng-Yan
FY
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D009369:Neoplasms / Q000175:diagnosis; D056921:Nuclear Receptor Coactivator 3 / Q000276:immunology*
D000911:Antibodies, Monoclonal; C089736:biotin 1; D001710:Biotin; C538866:NCOA3 protein, human; D056921:Nuclear Receptor Coactivator 3