[HPLC fingerprint of the antiarrhythmic fraction of Valeriana officinalis].
OBJECTIVE
To establish HPLC fingerprints of the Antiarrhythmic fraction of Valeriana officinalis.


METHODS
Agilent C18 (250 mm x 4.6 mm, 5 microm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was 380 degrees C and the detection wavelength was 218 nm. The detection time was 70 min, and the flow rate was 1.0 mL/ min.


RESULTS
Fifteen characteristic peaks were indicated in HPLC fingerprints. The relative retention time and the ranges of relative areas of the common peaks were also determined.


CONCLUSIONS
This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of Valeriana officinalis.
Duan
Xue-Yun
XY
Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Hubei College of TCM, Ministry of Education, Wuhan 430061, China. xueyunduan@sina.com
Gong
Zhan-Feng
ZF
Chen
Shu-He
SH
Fang
Ying
Y
Liu
Yan-Wen
YW
D000889:Anti-Arrhythmia Agents / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D014632:Valerian / Q000737:chemistry* / Q000145:classification / Q000254:growth & development
D000889:Anti-Arrhythmia Agents; D004365:Drugs, Chinese Herbal