title: Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization.
abstract: We have developed 2'-O-methyl-RNAs having phosphorylated cyclohexane at the 5' and/or 3'-terminal adenine. These 2'-O-methyl-RNAs formed less stable duplex with the longer target RNA than that with the short target RNA. We tried to improve the short-RNA selective hybridization property by the synthesis of 2'-O-methyl-RNAs having terminal guanine modifications.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. kseio@bio.titech.ac.jp
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Kazuya
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Sayako
authors.initials: S
authors.lastname: Kurohagi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Masaki
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tsunoda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D003510:Cyclohexanes / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D022661:RNA, Untranslated / Q000737:chemistry*
chemical_list: D003510:Cyclohexanes; D009841:Oligonucleotides; D022661:RNA, Untranslated; C506365:Cyclohexane