Identification of recombinant human papillomavirus type 16 variants.
Intratypic diversity of human papillomavirus (HPV) genome is generally characterized by point mutation, insertion, and/or deletion. Using PCR-based cloning and sequencing, we detected concurrent infection with 8 HPV16 variants in a woman enrolled in the ASCUS-LSIL Triage Study. The European variant was the major variant; each of the 7 minor variants had partial DNA sequences identical to the European variant and another part identical to the African 2 variant. At a follow-up visit, only an HPV16 African 2 variant was detected. Results from the present study suggest presence of intratypic recombination of HPV genome in natural infection.
Jiang
Mingjun
M
Department of Pathology, School of Medicine, Seattle, WA 98105, USA.
Xi
Long Fu
LF
Edelstein
Zoe R
ZR
Galloway
Denise A
DA
Olsem
Gary J
GJ
Lin
William Chun-Che
WC
Kiviat
Nancy B
NB
D004279:DNA, Viral / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D055815:Young Adult
D004279:DNA, Viral