Enteric co-innervation of esophageal striated muscle fibers: a phylogenetic study.
Enteric co-innervation of striated muscle fibers in the esophagus occurs in several mammalian species including humans. However, the functional significance is still unknown. Phylogenetic data may be instrumental in gaining further insight. We examined the bat Glossophaga soricina and the shrew Suncus murinus as representatives for phylogenetically old mammals. As ruminants the antelope Tragelaphus imberbis, the he-goat Capra falconeri and the sheep Ovis aries were selected. As non-mammals the clawed frog Xenopus laevis as representative for the taxon amphibian and the rainbow trout Oncorhynchus mykiss as representative for the taxon fish were included. Histochemistry for nicotinamide adenine dinucleotide phosphate-diaphorase and acetylcholinesterase as well as immunofluorescence for vasoactive intestinal peptide and alpha-bungarotoxin were used to demonstrate enteric nerve fibers and motor endplates, respectively. Motor endplates were associated with enteric nerve fibers in all species investigated, although the rates of co-innervation varied from approximately 10 to 20% in shrew, antelope, he-goat, frog and fish, approximately 40% in bat to nearly 90% in sheep. These results demonstrate that enteric co-innervation, in spite of varying co-innervation rates, is conserved through vertebrate evolution, and underline the significance of this newly discovered innervation component.
Hempfling
Christina
C
Institute of Anatomy, University of Erlangen-Nuremberg, Krankenhausstrasse 9, D-91054 Erlangen, Germany.
Seibold
Ralf
R
Shiina
Takahiko
T
Heimler
Wolfgang
W
Neuhuber
Winfried L
WL
Wörl
Jürgen
J
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000866:Antelopes / Q000033:anatomy & histology / Q000502:physiology; D005075:Biological Evolution; D002685:Chiroptera / Q000033:anatomy & histology / Q000502:physiology; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D017615:Enteric Nervous System / Q000033:anatomy & histology* / Q000502:physiology; D004947:Esophagus / Q000294:innervation* / Q000502:physiology; D006041:Goats / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D054792:Muscle, Striated / Q000294:innervation* / Q000502:physiology; D009411:Nerve Endings / Q000502:physiology / Q000648:ultrastructure*; D009469:Neuromuscular Junction / Q000033:anatomy & histology* / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology / Q000502:physiology; D010802:Phylogeny; D012756:Sheep / Q000033:anatomy & histology / Q000502:physiology; D012788:Shrews / Q000033:anatomy & histology / Q000502:physiology; D013045:Species Specificity; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D014981:Xenopus / Q000033:anatomy & histology / Q000502:physiology
D014660:Vasoactive Intestinal Peptide; D008058:Dihydrolipoamide Dehydrogenase; D000110:Acetylcholinesterase