Cell phenotyping in saliva of individuals under psychological stress.
Total leukocytes, NK cells, B and T lymphocytes present in the saliva of medical students with or without stress were quantified by flow cytometry in 10,000 events. The symptoms of psychological stress were monitored with Lipp's Inventory of Stress Symptoms for Adults (ISSL). No significant differences were observed in the number of cells phenotyped in students with and those without psychological stress. However, a negative correlation was observed between the number of NK cells and T lymphocytes in students with stress (r=-0.8173; p=0.0058), suggesting that innate immunity is predominant in the adaptation phase.
Dos-Santos
Maria Cristina
MC
Laboratório de Imunologia, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus Amazonas, Brazil. mcsantos@ufam.edu.br
Matos-Gomes
Nathália
N
Makimoto
Fabiano Hiromichi
FH
Katsurayama
Marilise
M
Santana
Linda Luciana Oliveira
LL
Becker
Maria Alice d'Avila
MA
Paredes-Garcia
Edijane
E
Bertho
Alvaro L
AL
D000222:Adaptation, Physiological / Q000276:immunology*; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D003430:Cross-Sectional Studies; D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008297:Male; D010641:Phenotype; D012463:Saliva / Q000166:cytology / Q000276:immunology*; D013315:Stress, Psychological / Q000276:immunology* / Q000523:psychology; D013337:Students, Medical / Q000523:psychology; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult