title: The impact of antigen expression in antigen-presenting cells on humoral immune responses against the transgene product.
abstract: Treatment of genetic diseases by gene therapy is hampered by immune responses against the transgene product. Promoter choice has been shown to be an important parameter of the presence or absence of antibodies against the transgene product after gene transfer. Here, the generality of some of these observations was tested by comparing different murine strains and different transgene products. We show immunological unresponsiveness for human apolipoprotein (apo) A-I in six murine strains after transfer with E1E3E4-deleted adenoviral vectors containing hepatocyte-specific expression cassettes. However, differences in the induction of a humoral immune response against human apo A-I after gene transfer with vectors driven by the major histocompatibility complex class II Ebeta promoter and the ubiquitously active cytomegalovirus promoter were not consistent in these six murine strains. Furthermore, use of a potent hepatocyte-specific expression cassette did not prevent a humoral immune response against human plasminogen in C57BL/6 mice. In contrast, human microplasminogen transfer resulted in stable expression in the absence of an immune response against the transgene product. Taken together, the molecular design of strategies to abrogate or induce an immune response against the transgene product may be hampered by the multitude of parameters affecting the outcome, thus limiting the external validity of results.
authors.affiliation: Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium.
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: F
authors.initials: F
authors.lastname: Jacobs
authors.forename: E
authors.initials: E
authors.lastname: Van Craeyveld
authors.forename: J
authors.initials: J
authors.lastname: Lievens
authors.forename: J
authors.initials: J
authors.lastname: Snoeys
authors.forename: S
authors.initials: S
authors.lastname: Van Linthout
authors.forename: B
authors.initials: B
authors.lastname: De Geest
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D016632:Apolipoprotein A-I / Q000276:immunology*; D003587:Cytomegalovirus / Q000235:genetics; D018014:Gene Transfer Techniques; D005802:Genes, MHC Class II; D005822:Genetic Vectors; D022781:Hepatocytes / Q000276:immunology; D006801:Humans; D056724:Immunity, Humoral; D051379:Mice; D008815:Mice, Inbred Strains; D010446:Peptide Fragments / Q000235:genetics; D010958:Plasminogen / Q000235:genetics; D011401:Promoter Regions, Genetic; D019076:Transgenes
chemical_list: D016632:Apolipoprotein A-I; D010446:Peptide Fragments; C111879:microplasminogen protein, human; D010958:Plasminogen