Genetic loci that regulate healing and regeneration in LG/J and SM/J mice.
MRL mice display unusual healing properties. When MRL ear pinnae are hole punched, the holes close completely without scarring, with regrowth of cartilage and reappearance of both hair follicles and sebaceous glands. Studies using (MRL/lpr x C57BL/6)F(2) and backcross mice first showed that this phenomenon was genetically determined and that multiple loci contributed to this quantitative trait. The lpr mutation itself, however, was not one of them. In the present study we examined the genetic basis of healing in the Large (LG/J) mouse strain, a parent of the MRL mouse and a strain that shows the same healing phenotype. LG/J mice were crossed with Small (SM/J) mice and the F(2) population was scored for healing and their genotypes determined at more than 200 polymorphic markers. As we previously observed for MRL and (MRL x B6)F(2) mice, the wound-healing phenotype was sexually dimorphic, with female mice healing more quickly and more completely than male mice. We found quantitative trait loci (QTLs) on chromosomes (Chrs) 9, 10, 11, and 15. The heal QTLs on Chrs 11 and 15 were linked to differential healing primarily in male animals, whereas QTLs on Chrs 9 and 10 were not sexually dimorphic. A comparison of loci identified in previous crosses with those in the present report using LG/J x SM/J showed that loci on Chrs 9, 11, and 15 colocalized with those seen in previous MRL crosses, whereas the locus on Chr 10 was not seen before and is contributed by SM/J.
Blankenhorn
Elizabeth P
EP
Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA> eblanken@drexelmed.edu
Bryan
Gregory
G
Kossenkov
Andrew V
AV
Clark
Lise Desquenne
LD
Zhang
Xiang-Ming
XM
Chang
Celia
C
Horng
Wenhwai
W
Pletscher
L Susan
LS
Cheverud
James M
JM
Showe
Louise C
LC
Heber-Katz
Ellen
E
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004423:Ear / Q000293:injuries; D005260:Female; D006239:Haplotypes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018895:Microsatellite Repeats; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012038:Regeneration / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics; D014945:Wound Healing / Q000235:genetics*
D012313:RNA