title: Biomechanical and radiographic comparison of demineralized bone matrix, and a coralline hydroxyapatite in a rabbit spinal fusion model.
abstract: The use of bone grafts is an essential component in spinal fusion. Autologous bone has been shown to result in long-term stable arthrodesis between spinal motion segments. However, autograft can be associated with significant morbidity and a limited supply. Alternatives, such as allogeneic demineralized bone matrix (DBM), are a potential source and supplement to autograft bone. The current study compares the ability of a DBM product (BioSet RT) and a coralline hydroxyapatite (Pro Osteon 500R), for inducing spinal fusion in a rabbit model. BioSet RT, alone or in combination with autograft, and Pro Osteon 500R were implanted in the posterior lateral inter-transverse process region of the rabbit spine. The spines were evaluated at 18 weeks for fusion of the L4-L5 transverse processes using a total of 33 skeletally mature male rabbits; 4 naïve animals were also included in the study. Samples were evaluated radiographically, histologically, by palpation, and through mechanical strength testing. Radiographical, histological, and palpation measurements demonstrated the ability of BioSet RT to induce new bone formation and bridging fusion comparable to autograft. This material performed well alone or in combination with autograft material. Despite significantly higher biomechanical testing results, minimal bone formation and fusion was recorded for the Pro Osteon 500R-treated group. This in vivo study demonstrates the ability of BioSet RT to induce new bone formation, and there was a clear relationship between bridging bone and mechanical strength.
authors.affiliation: RTI Biologics, Alachua, FL, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Dodds
authors.forename: Amanda M
authors.initials: AM
authors.lastname: York-Ely
authors.forename: Rasa
authors.initials: R
authors.lastname: Zhukauskas
authors.forename: Travis
authors.initials: T
authors.lastname: Arola
authors.forename: John
authors.initials: J
authors.lastname: Howell
authors.forename: Caroline
authors.initials: C
authors.lastname: Hartill
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Cobb
authors.forename: Casey
authors.initials: C
authors.lastname: Fox
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D018786:Bone Substitutes / Q000737:chemistry / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000000981:diagnostic imaging* / Q000379:methods; D002516:Ceramics / Q000737:chemistry / Q000627:therapeutic use*; D006882:Hydroxyapatites / Q000737:chemistry / Q000627:therapeutic use*; D010012:Osteogenesis; D011817:Rabbits; D011859:Radiography; D013123:Spinal Fusion / Q000379:methods; D013131:Spine / Q000000981:diagnostic imaging / Q000601:surgery / Q000648:ultrastructure; D014182:Transplantation, Autologous
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; C119955:coralline hydroxyapatite