[Detection of specific interferon-gamma-secreting T cell response to Mycobacterium tuberculosis RD1-encoded antigens in pleural effusions, ascites, and cerebrospinal fluid].
OBJECTIVE
To detect Mycobacterium tuberculosis RD1-encoded antigens-specific, interferon-gamma (INF-gamma)-secreting T cells in pleural effusions, ascites, and cerebrospinal fluid.


METHODS
The early secretory antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) peptides-specific T cells in peripheral blood mononuclear cell (MC), ascites MC, pleural effusions MC, and cerebrospinal fluid MC were detected using enzyme-linked immunospot assay (ELISPOT) for INF-gamma.


RESULTS
ESAT-6 or CFP-10 peptides-specific, INF-gamma-secreting T cells were detected in peripheral blood, ascites, pleural effusions, and cerebrospinal fluid, which marked the presence of tuberculosis infection. Patients with positive ELISPOT results of INF-gamma-release assay were all diagnosed as active tuberculosis. Spot forming cells in ascites and pleural effusions were much higher than those in peripheral blood (up to 6.4 and 31.9 times).


CONCLUSIONS
Detection of RD1-encoded antigens-specific, INF-gamma-secreting T cells in pleural effusions, ascites, and cerebrospinal fluid provides a new way to diagnose tuberculosis infection.
Zhang
Li-Fan
LF
Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Liu
Xiao-Qing
XQ
D000942:Antigens, Bacterial / Q000235:genetics; D001201:Ascites / Q000378:metabolism; D001426:Bacterial Proteins; D006801:Humans; D007371:Interferon-gamma / Q000134:cerebrospinal fluid / Q000378:metabolism*; D007963:Leukocytes, Mononuclear; D009169:Mycobacterium tuberculosis; D010455:Peptides; D010996:Pleural Effusion / Q000276:immunology*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010455:Peptides; D011994:Recombinant Proteins; C072681:Mycobacterium tuberculosis antigens; D007371:Interferon-gamma