Development of light-responsive BNA toward the spatiotemporal control of gene expression.
We designed and synthesized light-responsive BNA (bridged nucleic acid), which has a photolabile group at the bridged structure. Light-Responsive BNA can change its structure by a two stages process: photoirradiation followed by nucleophilic treatment. In oligonucleotides containing light-responsive BNA moieties, binding affinity with complementary single-stranded RNA was lost upon photoirradiation, but could be restored by nucleophilic treatment.(1)
Morihiro
Kunihiko
K
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
Kodama
Tetsuya
T
Nishida
Masaru
M
Imanishi
Takeshi
T
Obika
Satoshi
S
D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D008027:Light; D009841:Oligonucleotides / Q000737:chemistry
D001952:Bridged-Ring Compounds; D009841:Oligonucleotides