Oxidative stress induced by morphine in brain of rats fed with a protein deficient diet.
The objective of the study is to determine the damage by oxidative stress induced by morphine in brain of rats fed with a protein-deficient diet. Twenty-eight malnourished male Wistar rats, 30 days old, were used in the study. The animals were divided into four groups of 7 rats per group. Group I received NaCl and the groups II; III and IV intraperitoneally received 3, 6 and 12 mg/kg of morphine sulphate, respectively, in a single dose. Animals were sacrificed and the levels of glutathione (GSH), dopamine, tryptophan and 5-hydroxyindole-3-acetic acid (5-HIAA) as well as, Na(+)/K(+) ATPase and total ATPase activity in the brain were measured. Tryptophan levels and Na(+)/K( +) ATPase activity showed non-significant changes in the experimental group. Levels of 5-HIAA decreased significantly (p = .03) in animals that received 12 mg/kg of morphine and in animals that received 3 mg/kg, levels of GSH and dopamine were found to have a significant decrease (p < .05), but a significant increase in the group that received 12 mg/kg of morphine (p < .05). Total ATPase activity increased significantly in the groups that received 3 mg/kg (p = .015) and 6 mg/kg (p = .0001) of morphine. The results show that malnutrition induces changes in cellular regulation and biochemical responses to oxidative stress caused by morphine sulphate.
Calderón-Guzmán
David
D
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, México.
Osnaya-Brizuela
Norma
N
García-Alvarez
Raquel
R
Hernández-García
Ernestina
E
Juárez-Olguín
Hugo
H
D000701:Analgesics, Opioid / Q000009:adverse effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008297:Male; D009020:Morphine / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011488:Protein Deficiency / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000701:Analgesics, Opioid; D000975:Antioxidants; D015415:Biomarkers; D009020:Morphine