title: Detoxification of mercury species--an in vitro study with antidotes in human whole blood.
abstract: To investigate the effects of mercury species intoxication and to test the efficiency of different commonly applied antidotes, human whole blood and plasma surrogate samples were spiked with inorganic mercury (Hg2+) and methylmercury (MeHg+, CH3Hg+) prior to treatment with the antidotes 2,3-dimercaptopropan-1-ol (British Anti Lewisite), 2,3-dimercaptosuccinic acid (DMSA), and N-acetylcysteine (NAC). For mercury speciation analysis in these samples, liquid chromatography was coupled to either inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionisation time-of-flight mass spectrometry (ESI-TOF-MS). Adduct formation between mercury species and physiological thiols (cysteine and glutathione) was observed as well as the release of glutathione under treatment with the antidotes DMSA and NAC.
authors.affiliation: University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 30, 48149 Münster, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Trümpler
authors.forename: Sascha
authors.initials: S
authors.lastname: Nowak
authors.forename: Björn
authors.initials: B
authors.lastname: Meermann
authors.forename: Gerhard A
authors.initials: GA
authors.lastname: Wiesmüller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Buscher
authors.forename: Michael
authors.initials: M
authors.lastname: Sperling
authors.forename: Uwe
authors.initials: U
authors.lastname: Karst
mesh_terms: D000328:Adult; D000931:Antidotes / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008658:Inactivation, Metabolic; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008628:Mercury / Q000097:blood / Q000493:pharmacokinetics*; D008767:Methylmercury Compounds / Q000097:blood / Q000493:pharmacokinetics*; D008954:Models, Biological
chemical_list: D000931:Antidotes; D008767:Methylmercury Compounds; D008628:Mercury