title: Group IV phospholipase A(2)alpha controls the formation of inter-cisternal continuities involved in intra-Golgi transport.
abstract: The organization of intra-Golgi trafficking and the nature of the transport intermediates involved (e.g., vesicles, tubules, or tubular continuities) remain incompletely understood. It was recently shown that successive cisternae in the Golgi stack are interconnected by membrane tubules that form during the arrival of transport carriers from the endoplasmic reticulum. Here, we examine the mechanisms of generation and the function of these tubules. In principle, tubule formation might depend on several protein- and/or lipid-based mechanisms. Among the latter, we have studied the phospholipase A(2) (PLA(2))-mediated generation of wedge-shaped lysolipids, with the resulting local positive membrane curvature. We show that the arrival of cargo at the Golgi complex induces the recruitment of Group IVA Ca(2+)-dependent, cytosolic PLA(2) (cPLA(2)alpha) onto the Golgi complex itself, and that this cPLA(2)alpha is required for the formation of the traffic-dependent intercisternal tubules and for intra-Golgi transport. In contrast, silencing of cPLA(2)alpha has no inhibitory effects on peri-Golgi vesicles. These findings identify cPLA(2)alpha as the first component of the machinery that is responsible for the formation of intercisternal tubular continuities and support a role for these continuities in transport through the Golgi complex.
authors.affiliation: Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Chieti, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: San Pietro
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Capestrano
authors.forename: Elena V
authors.initials: EV
authors.lastname: Polishchuk
authors.forename: Alessio
authors.initials: A
authors.lastname: DiPentima
authors.forename: Alvar
authors.initials: A
authors.lastname: Trucco
authors.forename: Pasquale
authors.initials: P
authors.lastname: Zizza
authors.forename: Stefania
authors.initials: S
authors.lastname: Mariggiò
authors.forename: Teodoro
authors.initials: T
authors.lastname: Pulvirenti
authors.forename: Michele
authors.initials: M
authors.lastname: Sallese
authors.forename: Stefano
authors.initials: S
authors.lastname: Tete
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Mironov
authors.forename: Christina C
authors.initials: CC
authors.lastname: Leslie
authors.forename: Daniela
authors.initials: D
authors.lastname: Corda
authors.forename: Alberto
authors.initials: A
authors.lastname: Luini
authors.forename: Roman S
authors.initials: RS
authors.lastname: Polishchuk
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004285:Dogs; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D054513:Group IV Phospholipases A2 / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D021381:Protein Transport; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D055571:Secretory Pathway; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D054513:Group IV Phospholipases A2; D002118:Calcium