Single-domain antibody-nanoparticles: promising architectures for increased staphylococcus aureus detection specificity and sensitivity.
Because antibodies are highly target-specific and nanoparticles possess diverse, material-dependent properties that can be exploited in order to label and potentially identify biomolecules, the development of antibody-nanoparticle conjugates (nanoconjugates) has huge potential in biodiagnostics. Here, we describe a novel superparamagnetic nanoconjugate, one whose recognition component is a single-domain antibody. It is highly active toward its target Staphylococcus aureus, displays long shelf life, lacks cross-reactivity inherent to traditional homologue whole antibodies, and captures a few dozen S. aureus cells in a mixed cell population with ~100% efficiency and specificity. We ascribe the excellent performance of our nanoconjugate to its single-domain antibody component and recommend it as a general purpose recognition element.
Ryan
Shannon
S
Institute for Biological Sciences, Steacie Institute for Molecular Sciences, and Institute for Microstructural Sciences, National Research Council Canada, Ottawa, Ontario, Canada.
Kell
Arnold J
AJ
van Faassen
Henk
H
Tay
Li-Lin
LL
Simard
Benoit
B
MacKenzie
Roger
R
Gilbert
Michel
M
Tanha
Jamshid
J
D000907:Antibodies, Bacterial / Q000737:chemistry* / Q000276:immunology; D001431:Bacteriological Techniques / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D058726:Nanoconjugates; D017434:Protein Structure, Tertiary; D012475:Salmonella; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D058726:Nanoconjugates; C000499:ferric oxide; D012822:Silicon Dioxide