Evaluation of the muscle gene transfer activity of a series of amphiphilic triblock copolymers.
BACKGROUND
Amphiphilic triblock copolymers such as the polyethylene oxide-polypropylene oxide-polyethylene oxide L64 (PEO(13)-PPO(30)-PEO(13)) significantly increase transgene expression after injection of DNA/polymer mixtures into skeletal muscles. To better understand the way such copolymers act, we studied the behaviour of different poloxamers, including L64, both in vitro and in vivo.


METHODS
The in vitro and in vivo transfection activity of five copolymers that differ either by their molecular weight or by their hydrophilic/hydrophobic balance was evaluated. Furthermore, we also studied the membrane permeabilizing properties of the poloxamers.


RESULTS
The results obtained indicate that, after intramuscular administration of DNA/poloxamer formulations, all five compounds were able to significantly increase the expression levels of luciferase compared to an injection of naked DNA. Using a LacZ expression cassette, up to 30% of the muscle fibers expressed the reporter gene. Furthermore, we show that the effect can be obtained using different promoters. Finally, we document that, to some extent, all five poloxamers possess membrane permeabilizing properties.


CONCLUSIONS
Taken together, the results obtained in the present study show that there is a large flexibility in terms of molecular weight and EO/PO ratio for obtaining increased levels of transgene expression in vivo.
Alimi-Guez
Debborah
D
FRE3087 CNRS-Genethon, Evry, France.
Leborgne
Christian
C
Pembouong
Gaelle
G
Van Wittenberghe
Laetitia
L
Mignet
Nathalie
N
Scherman
Daniel
D
Kichler
Antoine
A
D000818:Animals; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics*; D006801:Humans; D007273:Injections, Intramuscular; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism*; D032446:Myoblasts / Q000378:metabolism; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D014162:Transfection
D011108:Polymers; D004247:DNA; D007770:L-Lactate Dehydrogenase