Analysis of cell type-specific expression of CK1 epsilon in various tissues of young adult BALB/c Mice and in mammary tumors of SV40 T-Ag-transgenic mice.
Casein kinase 1 epsilon (CK1epsilon) is involved in various cellular processes, including cell growth, differentiation, and apoptosis, vesicle transport, and control of the circadian rhythm. Deregulation of CK1epsilon has been linked to neurodegenerative diseases and cancer. To better understand the cell type-specific functions of CK1epsilon, we determined its localization by immunhistochemistry in tissues of healthy, young adult BALB/c mice and in mammary tumors of SV40 T-antigen-transgenic mice. CK1epsilon expression was found to be highly regulated in normal tissues of endodermal, mesodermal, and ectodermal origin and in neoplastic tissue of mammary cancer. The data presented here give an overview of CK1epsilon reactivity in different organs under normal conditions and outline changes in its expression in mammary carcinomas. Our data suggest a cell/organ type-specific function of CK1epsilon and indicate that tumorigenic conversion of mammary glands in SV40 T-antigen-transgenic mice leads to downregulation of CK1epsilon. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
Utz
Anja C
AC
Department of General, Visceral, and Transplantation Surgery, University of Ulm, Steinhövelstr. 9, 89075 Ulm, Germany.
Hirner
Heidrun
H
Blatz
Annette
A
Hillenbrand
Andreas
A
Schmidt
Bernhard
B
Deppert
Wolfgang
W
Henne-Bruns
Doris
D
Fischer
Dietmar
D
Thal
Dietmar R
DR
Leithäuser
Frank
F
Knippschild
Uwe
U
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D048149:Casein Kinase 1 epsilon / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004475:Ectoderm / Q000201:enzymology; D004707:Endoderm / Q000201:enzymology; D005260:Female; D008297:Male; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000502:physiology; D015674:Mammary Neoplasms, Animal / Q000201:enzymology / Q000821:virology*; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology* / Q000235:genetics; D008648:Mesoderm / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009928:Organ Specificity
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D048149:Casein Kinase 1 epsilon