title: Biomimetic conformation-specific assembly of proteins at artificial binding sites nanopatterned on silicon.
abstract: Biomolecules such as enzymes and antibodies possess binding sites where the molecular architecture and the physicochemical properties are optimum for their interaction with a particular target, in some cases even differentiating between stereoisomers. Here, we mimic this exquisite specificity via the creation of a suitable chemical environment by fabricating artificial binding sites for the protein calmodulin (CaM). By downscaling well-known surface chemical modification methodologies to the nanometer scale via silicon nanopatterning, the Ca(2+)-CaM conformer was found to selectively bind the biomimetic binding sites. The methodology could be adapted to mimic other protein-receptor interactions for sensing and catalysis.
authors.affiliation: Department of Chemistry and Biochemistry, City University of New York, Hunter College, 695 Park Avenue, New York, New York 10065, USA. roberto.delarica@gmail.com
authors.forename: Roberto
authors.initials: R
authors.lastname: de la Rica
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsui
mesh_terms: D001665:Binding Sites; D040761:Biomimetic Materials / Q000737:chemistry*; D002147:Calmodulin / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D012825:Silicon / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D002147:Calmodulin; D012825:Silicon