Depletion and mutation of topoisomerase II in animal cells.
Eukaryotic type II topoisomerases (Topo II) are implicated in a wide range of cellular processes. Cells in which Topo II protein has been specifically depleted or mutated provide powerful systems for analysing the normal in vivo functions of Topo II proteins and for assessing their roles in various chemotherapy regimens. Summarised here are the ways in which Topo II has been depleted or mutated in animal cells and the type of information gleaned. The cell lines generated are tabulated and represent a useful resource for further in vivo studies of Topo II function, one that we expect to grow in size and utility in the coming years.
Porter
Andrew C G
AC
Department of Haematology, Imperial College Faculty of Medicine, London, UK.
D000818:Animals; D002460:Cell Line; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D016296:Mutagenesis; D009154:Mutation; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D059005:Topoisomerase II Inhibitors
D007527:Isoenzymes; D034741:RNA, Small Interfering; D059005:Topoisomerase II Inhibitors; D004250:DNA Topoisomerases, Type II