title: Comparison of microbial growth inhibition screening assays with high-pressure liquid chromatography for detection of experimentally incurred penicillin G residues in calves.
abstract: Twelve calves were fed milk replacer containing 3.33 ppm penicillin G for one feeding, and 12 calves were fed the medicated milk replacer once daily at a rate of 12% of their body weight for 14 days. Two calves served as controls. Calves were slaughtered 4 to 13 hours after being fed. Kidney, liver, muscle, and urine samples were assayed for penicillin by high-pressure liquid chromatography (HPLC). Penicillin G was not detected in any muscle samples and concentrations of penicillin exceeded the tolerance level (0.05 microg/g) in kidney or liver tissue from 13 of 24 treated-calf carcasses examined by HPLC. The Live Animal Swab Test identified all 13 carcasses with violative drug residues. Using kidney as the test matrix, the Swab Test on Premises identified 10 of 13 carcasses with violative drug residues, while the Calf Antibiotic Sulfa Test identified seven of 13 carcasses with violative residues.
authors.affiliation: Texas A&M University, Texas Veterinary Medical Center, Department of Veterinary Pathobiology, College Station, TX 77843, USA.
authors.forename: Jeffrey M B
authors.initials: JM
authors.lastname: Musser
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Anderson
authors.forename: Joe O
authors.initials: JO
authors.lastname: Boison
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001681:Biological Assay; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D041941:Milk Substitutes / Q000737:chemistry; D010400:Penicillin G / Q000737:chemistry* / Q000378:metabolism / Q000652:urine
chemical_list: D000900:Anti-Bacterial Agents; D010400:Penicillin G