title: Subinhibitory concentrations of moxifloxacin decrease adhesion and biofilm formation of Stenotrophomonas maltophilia from cystic fibrosis.
abstract: Stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen that is currently isolated with increasing frequency from the airways of cystic fibrosis (CF) patients. In this study the effect of subinhibitory concentrations (subMICs) of moxifloxacin on adhesion, biofilm formation and cell-surface hydrophobicity of two strains of S. maltophilia isolated from CF patients were evaluated. Adhesion and biofilm formation assays were carried out on polystyrene and quantified by colony counts. Cell-surface hydrophobicity was determined by a test for adhesion to n-hexadecane. Moxifloxacin at 0.03x and 0.06x MIC caused a significant decrease in adhesion and biofilm formation by both strains tested. A significant reduction in cell-surface hydrophobicity following exposure to subMICs of moxifloxacin was observed for one strain only. The results of the present study provide an additional rationale for the use of moxifloxacin in CF patients and more generally in biofilm-related infections involving S. maltophilia.
authors.affiliation: Center for Excellence on Aging, 'G. D'Annunzio' University Foundation, Chieti, Italy.|Clinical Microbiology Unit, Department of Biomedical Sciences, 'G. D'Annunzio' University of Chieti-Pescara, Chieti, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Pompilio
authors.affiliation: Clinical Microbiology and Virology Unit, Department of Transfusion Medicine, 'Spirito Santo' Hospital, Pescara, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Catavitello
authors.affiliation: Center for Excellence on Aging, 'G. D'Annunzio' University Foundation, Chieti, Italy.|Clinical Microbiology Unit, Department of Biomedical Sciences, 'G. D'Annunzio' University of Chieti-Pescara, Chieti, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Picciani
authors.affiliation: Center for Excellence on Aging, 'G. D'Annunzio' University Foundation, Chieti, Italy.|Clinical Microbiology Unit, Department of Biomedical Sciences, 'G. D'Annunzio' University of Chieti-Pescara, Chieti, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Confalone
authors.affiliation: Center for Excellence on Aging, 'G. D'Annunzio' University Foundation, Chieti, Italy.|Clinical Microbiology Unit, Department of Biomedical Sciences, 'G. D'Annunzio' University of Chieti-Pescara, Chieti, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Piccolomini
authors.affiliation: Clinical Microbiology and Virology Unit, Department of Transfusion Medicine, 'Spirito Santo' Hospital, Pescara, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Savini
authors.affiliation: Laboratory of Clinical Microbiology, 'Bambino Gesù' Pediatric Hospital, Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Fiscarelli
authors.affiliation: Clinical Microbiology and Virology Unit, Department of Transfusion Medicine, 'Spirito Santo' Hospital, Pescara, Italy.
authors.forename: D
authors.initials: D
authors.lastname: D'Antonio
authors.affiliation: Center for Excellence on Aging, 'G. D'Annunzio' University Foundation, Chieti, Italy.|Clinical Microbiology Unit, Department of Biomedical Sciences, 'G. D'Annunzio' University of Chieti-Pescara, Chieti, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Di Bonaventura
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001372:Aza Compounds / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D003550:Cystic Fibrosis / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones; D006801:Humans; D000077266:Moxifloxacin; D011804:Quinolines / Q000494:pharmacology*; D020615:Stenotrophomonas maltophilia / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D000077266:Moxifloxacin