Identification of colonization factors of enterotoxigenic Escherichia coli with PCR-based technique.
Colonization factors (CFs) mediate attachment of enterotoxigenic Escherichia coli (ETEC) to the intestinal mucosa and induce protective immunity against ETEC diarrhoea. We designed CF-specific polymerase chain reaction (PCR) primers, and developed a simple PCR-based genotypic CF identification method. ETEC strains (n=17) isolated from patients with diarrhoea in Thailand were examined for genotypical identification of CFs of ETEC strains. Coli surface antigen 6 (CS6) was the most common CF (29%), followed by CS13 (12%), colonization factor antigen I (CFA/I), CS2 and CS3, and CS17/CS19 (6% each), while 41% of the strains were negative. This simple PCR method for the detection of CF genes is useful for surveillance of ETEC infections in diagnostic laboratories.
Puiprom
O
O
Section of Bacterial Infections, Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.
Chantaroj
S
S
Gangnonngiw
W
W
Okada
K
K
Honda
T
T
Taniguchi
T
T
Sawanpanyalert
P
P
D001431:Bacteriological Techniques / Q000379:methods*; D003967:Diarrhea / Q000382:microbiology; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013785:Thailand; D037521:Virulence Factors / Q000235:genetics*
D029968:Escherichia coli Proteins; D037521:Virulence Factors; C022924:colonization factor antigens; D036781:Fimbriae Proteins