title: [Purification of EGCG monomer from the extracts of tea by high speed countercurrent chromatography].
abstract: OBJECTIVE
  To investigate the feasibility and techniques for purifying epigallocatechin-3-gallate (EGCG) by high speed countercurrent chromatography (HSCCC).
  
  
  METHODS
  A HSCCC unit was used for the purification of EGCG from the extracts of tea.
  
  
  RESULTS
  The preferable solvent system was composed of Ether-Ethyl Acetate-H2O (4:10: 25, V/V), and the preferable technical conditions were as follows: the temperature was 35 degrees C, the rotation speed was 800 r/min and the mobile phase flow-rate was 2.5 mL/min.
  
  
  CONCLUSIONS
  The purity of the obtained EGCG sample determined by high performance liquid chromatography (HPLC) is 95%.
authors.affiliation: School of Chemistry & Chemical Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Jiang
authors.forename: Shao-Lie
authors.initials: SL
authors.lastname: Huang
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Zheng
mesh_terms: D000085:Acetates / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D004986:Ether / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D013662:Tea / Q000737:chemistry*
chemical_list: D000085:Acetates; D010936:Plant Extracts; D012997:Solvents; D013662:Tea; D004986:Ether; D002392:Catechin; C045651:epigallocatechin gallate