Curcumin inhibits phorbol myristate acetate (PMA)-induced MCP-1 expression by inhibiting ERK and NF-kappaB transcriptional activity.
Monocyte chemoattractant protein-1 (MCP-1) is a potent mediator of macrophage migration and therefore, plays an essential role in early events of inflammation. In the present study, we show the protein kinase C activator, phorbol myristate acetate (PMA), potently induced mRNA expression and secretion of the C-C chemokine MCP-1 in U937 cells. We found that curcumin, a natural biologically active compound extracted from rhizomes of Curcuma species, significantly inhibited the PMA-induced increase in MCP-1 expression and secretion. These effects of curcumin are dose dependent and correlate with the suppression of MCP-1 mRNA expression levels. Curcumin inhibited PMA-mediated activation of extracellular signal-regulated kinase (ERK) and NF-kappaB transcriptional activity. Therefore, one possible anti-inflammatory mechanism of curcumin may be to inhibit the secretions of inflammatory MCP-1 chemokine.
Lim
Jun Hee
JH
Department of Immunology, School of Medicine, Keimyung University, 194 DongSan-Dong, Taegu 700-712, Republic of Korea.
Kwon
Taeg Kyu
TK
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D004396:Coloring Agents; D003474:Curcumin / Q000494:pharmacology*; D024202:Electrophoretic Mobility Shift Assay; D004797:Enzyme-Linked Immunosorbent Assay; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D020298:U937 Cells
D000970:Antineoplastic Agents; D018932:Chemokine CCL2; D004396:Coloring Agents; D016328:NF-kappa B; D013778:Tetrazolium Salts; D013844:Thiazoles; D018808:Transcription Factor AP-1; D012313:RNA; D008156:Luciferases; D048049:Extracellular Signal-Regulated MAP Kinases; C022616:thiazolyl blue; D003474:Curcumin; D013755:Tetradecanoylphorbol Acetate