Map-based cloning of the ERECT PANICLE 3 gene in rice.
Panicle architecture in rice can have a strong influence on yield. Using N-methyl-N-nitrosourea mutagenesis, we isolated an erect panicle mutant, Hep, from Hwasunchalbyeo, a glutinous japonica rice cultivar. Genetic analysis revealed that the erect panicle phenotype was controlled by a single recessive mutation designated erect panicle 3 (ep3). Genetic mapping revealed that the ep3 mutation was located on the short arm of chromosome 2 in a 0.1 cM region delimited by the STS markers STS5803-5 and STS5803-7. The ep3 locus corresponded to 46.8 kb region and contained six candidate genes. Comparison of the DNA sequences of the candidate genes from wild-type and erect panicle plants revealed a single base-pair change in the second exon of LOC_Os02g15950, which is predicted to result in a nonsense mutation. LOC_Os02g15950 encodes a putative F-box protein containing 515 amino acids and is expressed throughout the plant during all growth stages. A line carrying a T-DNA insertion in LOC_ Os02g15950 was obtained and shown to have the same phenotype as the ep3 mutant, thus confirming the identification of LOC_Os02g15950 as the ERECT PANICLE 3 (EP3) gene. The ep3 mutation causes a significant increase in the number of small vascular bundles as well as the thickness of parenchyma in the peduncle, which results in the erect panicle phenotype.
Piao
Rihua
R
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.
Jiang
Wenzhu
W
Ham
Tae-Ho
TH
Choi
Min-Seon
MS
Qiao
Yongli
Y
Chu
Sang-Ho
SH
Park
Jung-Hyun
JH
Woo
Mi-Ok
MO
Jin
Zhengxun
Z
An
Gynheung
G
Lee
Joohyun
J
Koh
Hee-Jong
HJ
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D044783:F-Box Proteins / Q000235:genetics*; D017343:Genes, Plant; D009154:Mutation; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*
D044783:F-Box Proteins; D010940:Plant Proteins