A comparison study on microwave-assisted extraction of Artemisia sphaerocephala polysaccharides with conventional method: Molecule structure and antioxidant activities evaluation.
The conventional extraction methods for polysaccharides were time-consuming, laborious and energy-consuming. Microwave-assisted extraction (MAE) technique was employed for the extraction of Artemisia sphaerocephala polysaccharides (ASP), which is a traditional Chinese food. The extracting parameters were optimized by Box-Behnken design. In microwave heating process, a decrease in molecular weight (M(w)) was detected in SEC-LLS measurement. A d(f) value of 2.85 indicated ASP using MAE exhibited as a sphere conformation of branched clusters in aqueous solution. Furthermore, it showed stronger antioxidant activities compared with hot water extraction. The data obtained showed that the molecular weights played a more important role in antioxidant activities.
Wang
Junlong
J
Bioactive Products Engineering Research Center For Gansu Distinctive Plants, Northwest Normal University, Lanzhou 730070, China.
Zhang
Ji
J
Wang
Xiaofang
X
Zhao
Baotang
B
Wu
Yiqian
Y
Yao
Jian
J
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D019306:Artemisia / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000737:chemistry; D002614:Chelating Agents / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008872:Microwaves; D015233:Models, Statistical; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013481:Superoxides / Q000737:chemistry; D013696:Temperature
D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl