title: Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin.
abstract: The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rha-(1-->, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-L-Rha-(1-->, which was identified in a previous study.
authors.affiliation: Forestry and Forest Products Research Institute, 1, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Konishi
authors.forename: Fumiko
authors.initials: F
authors.lastname: Taguchi
authors.forename: Masako
authors.initials: M
authors.lastname: Iwaki
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ohnishi-Kameyama
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Ikuko
authors.initials: I
authors.lastname: Maeda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishida
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ichinose
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ishii
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D044224:Pseudomonas syringae / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides