Characterization of heme coordination structure in heme-DNA complex possessing gaseous molecule as an exogenous ligand.
We have previously demonstrated that heme, iron(III)-protoporphyrin IX complex, and a parallel-quadruplex DNA assembled from d(TTAGGG) form a stable coordination complex called "heme-DNA complex". The heme-DNA complex exhibits a variety of spectroscopic characteristics remarkably similar to those of met-form of myoglobin, oxygen-binding hemoprotein, reflecting that the heme environments in the two systems are highly alike to each other. In a course of our effort toward exploring functional properties of the heme-DNA complex, we have investigated binding of gaseous molecules such as CO and O(2) to the heme-DNA complex. The present study revealed that the heme-DNA complex exhibits ability to accommodate these molecules as exogenous ligands.
Saito
Kaori
K
Department of Chemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan.
Nakano
Yusuke
Y
Tai
Hulin
H
Nagatomo
Shigenori
S
Hemmi
Hikaru
H
Mita
Hajime
H
Yamamoto
Yasuhiko
Y
D002248:Carbon Monoxide / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D005740:Gases / Q000737:chemistry; D006418:Heme / Q000737:chemistry*; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular; D010100:Oxygen / Q000737:chemistry; D011524:Protoporphyrins / Q000737:chemistry; D013057:Spectrum Analysis
D005740:Gases; D008024:Ligands; D011524:Protoporphyrins; C448299:iron protoporphyrin IX; D006418:Heme; D002248:Carbon Monoxide; D004247:DNA; D010100:Oxygen