title: Fluoride-induced changes in haem biosynthesis pathway, neurological variables and tissue histopathology of rats.
abstract: This study intended to determine the effects of various concentrations of fluoride (1, 10, 50 and 100 ppm) in drinking water for a period of 12 weeks on changes in haem biosynthesis pathway, oxidative stress and neurological variables supported by histopathological observations and fluoride in rats. The data indicates significant alterations in the parameters related to haeme synthesis pathway like inhibition of blood delta-aminolevulinic acid dehydratase, delta-aminolevulinic acid synthetase, oxidative stress like depletion of glutathione (GSH) and increase in oxidized glutathione (GSSG) and thiobarbituric acid reactive substances. These changes were accompanied by depletion in GSH:GSSG ratio, whole brain biogenic amine levels and a dose-dependent increase in fluoride concentration. Interestingly and most significantly, these changes were more pronounced at lower concentrations of fluoride compared with higher fluoride dose. Biochemical changes were supported by the histological observations, which also revealed that at high concentrations of fluoride, toxic effects and damages to organs were more pronounced. These changes support our earlier findings regarding the role of decreased ionic mobility of fluoride ion at higher concentrations, leading to less pronounced toxicity.
authors.affiliation: Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior-474 002, India.
authors.forename: Swapnila
authors.initials: S
authors.lastname: Chouhan
authors.forename: Vinay
authors.initials: V
authors.lastname: Lomash
authors.forename: S J S
authors.initials: SJ
authors.lastname: Flora
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000473:pathology; D005459:Fluorides / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D006418:Heme / Q000096:biosynthesis*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014867:Water / Q000737:chemistry
chemical_list: D001679:Biogenic Amines; D014867:Water; D006418:Heme; D005459:Fluorides