title: Sequence analysis of an isolate of minute virus of canines in China reveals the closed association with bocavirus.
abstract: In the present study, we have cloned and sequenced the nearly-full-length genome of minute virus of canines (MVC), SH26, in China. The genome of MVC, 5,132 nucleotides (nts) in length, contains three open reading frames (ORFs), which are 2,325-bp of NS1, 561-bp of NP1 and 2,112-bp of VP1/VP2 encoding three proteins of 774, 186 and 703 residues, respectively. Predicted amino acids sequence of NS1 of MVC has 44% identity with human bocavirus (HBoV) and human boacvirus 2 (HBoV2), NP1 has 48 and 45% identity with HBoV and HBoV2, VP1/VP2 has 45 and 46% identity with HBoV and HBoV2, respectively. Phylogenetic analysis showed that the present Chinese MVC strain was also closely clustered with the previous American and Japanese MVC isolates, and MVCs formed a different branch together with bovine parvovirus and HBoVs from other parvoviruses classified into Parvovirinae.
authors.affiliation: Zoonosis and Comparative Medicine Group, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, People's Republic of China. shantongling@163.com
authors.forename: T L
authors.initials: TL
authors.lastname: Shan
authors.forename: L
authors.initials: L
authors.lastname: Cui
authors.forename: X Q
authors.initials: XQ
authors.lastname: Dai
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: X G
authors.initials: XG
authors.lastname: Shang
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Kang
authors.forename: Q
authors.initials: Q
authors.lastname: Shen
authors.forename: Z B
authors.initials: ZB
authors.lastname: Yang
authors.forename: J G
authors.initials: JG
authors.lastname: Zhu
authors.forename: X G
authors.initials: XG
authors.lastname: Hua
mesh_terms: D000818:Animals; D052660:Bocavirus / Q000235:genetics* / Q000302:isolation & purification*; D002681:China; D004285:Dogs; D006801:Humans; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*