title: Map-based cloning of the ERECT PANICLE 3 gene in rice.
abstract: Panicle architecture in rice can have a strong influence on yield. Using N-methyl-N-nitrosourea mutagenesis, we isolated an erect panicle mutant, Hep, from Hwasunchalbyeo, a glutinous japonica rice cultivar. Genetic analysis revealed that the erect panicle phenotype was controlled by a single recessive mutation designated erect panicle 3 (ep3). Genetic mapping revealed that the ep3 mutation was located on the short arm of chromosome 2 in a 0.1 cM region delimited by the STS markers STS5803-5 and STS5803-7. The ep3 locus corresponded to 46.8 kb region and contained six candidate genes. Comparison of the DNA sequences of the candidate genes from wild-type and erect panicle plants revealed a single base-pair change in the second exon of LOC_Os02g15950, which is predicted to result in a nonsense mutation. LOC_Os02g15950 encodes a putative F-box protein containing 515 amino acids and is expressed throughout the plant during all growth stages. A line carrying a T-DNA insertion in LOC_ Os02g15950 was obtained and shown to have the same phenotype as the ep3 mutant, thus confirming the identification of LOC_Os02g15950 as the ERECT PANICLE 3 (EP3) gene. The ep3 mutation causes a significant increase in the number of small vascular bundles as well as the thickness of parenchyma in the peduncle, which results in the erect panicle phenotype.
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.
authors.forename: Rihua
authors.initials: R
authors.lastname: Piao
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Jiang
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Ham
authors.forename: Min-Seon
authors.initials: MS
authors.lastname: Choi
authors.forename: Yongli
authors.initials: Y
authors.lastname: Qiao
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Chu
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Mi-Ok
authors.initials: MO
authors.lastname: Woo
authors.forename: Zhengxun
authors.initials: Z
authors.lastname: Jin
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
authors.forename: Joohyun
authors.initials: J
authors.lastname: Lee
authors.forename: Hee-Jong
authors.initials: HJ
authors.lastname: Koh
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D044783:F-Box Proteins / Q000235:genetics*; D017343:Genes, Plant; D009154:Mutation; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*
chemical_list: D044783:F-Box Proteins; D010940:Plant Proteins