title: SNP detection using trityl mass tags.
abstract: A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption-ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya, Moscow, Russia.
authors.forename: Klara R
authors.initials: KR
authors.lastname: Birikh
authors.forename: Pablo L
authors.initials: PL
authors.lastname: Bernad
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Shmanai
authors.forename: Andrei D
authors.initials: AD
authors.lastname: Malakhov
authors.forename: Mikhail S
authors.initials: MS
authors.lastname: Shchepinov
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Korshun
mesh_terms: D015342:DNA Probes / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D009841:Oligonucleotides / Q000138:chemical synthesis; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014320:Triphenylmethyl Compounds / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D014320:Triphenylmethyl Compounds; C434331:phosphoramidite