Gene expression of (pro)renin receptor is upregulated in hearts and kidneys of rats with congestive heart failure.
Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, was associated with organ damage in the kidney. However, there has been little information for (P)RR in hearts. To investigate the regulation of (P)RR in heart failure, we examined the expression of (P)RR in hearts and kidneys of rats with congestive heart failure (CHF) due to coronary ligation by quantitative RT-PCR and immunohistochemistry. Significantly increased levels of (P)RR mRNA were found in the atrium, right ventricle, non-infarcted part of left ventricle, infarcted part of left ventricle and kidney of CHF rats, when compared with sham operated rats (about 1.6-fold, 1.4-fold, 1.6-fold, 1.7-fold and 1.5-fold, respectively). Expression levels of mRNAs encoding renin and angiotensinogen in these heart and kidney tissues were also increased in the CHF rats. Immunohistochemistry showed positive (P)RR immunostaining in the myocardium, the renal tubular cells, and vascular smooth muscle and endothelial cells in the heart and the kidney. The renal tubular cells were more intensely immunostained in CHF rats than in sham operated rats. These findings suggest that the expression of (P)RR is increased in the hearts and kidneys of rats with heart failure, and that (P)RR may contribute to heart failure.
Hirose
Takuo
T
Department of Clinical Pharmacology and Therapeutics, Tohoku University Graduate School of Pharmaceutical Sciences and Medicine, Sendai 980-8578, Japan.
Mori
Nobuyoshi
N
Totsune
Kazuhito
K
Morimoto
Ryo
R
Maejima
Takahiro
T
Kawamura
Takuya
T
Metoki
Hirohito
H
Asayama
Kei
K
Kikuya
Masahiro
M
Ohkubo
Takayoshi
T
Kohzuki
Masahiro
M
Takahashi
Kazuhiro
K
Imai
Yutaka
Y
D000808:Angiotensinogen / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D006333:Heart Failure / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011921:Rats, Inbred WKY; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012083:Renin / Q000235:genetics; D012084:Renin-Angiotensin System / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D000093342:Prorenin Receptor
D011956:Receptors, Cell Surface; D000808:Angiotensinogen; D012083:Renin; D000093342:Prorenin Receptor