[Effects of Dan-Chuan-Hong decoction on myocardial apoptosis of acute myocardial ischemia in rats].
OBJECTIVE
To observe the effects of Dan-Chuan-Hong decoction on myocardial apoptosis of acute myocardial ischemia in rats.


METHODS
Rats received Guan-Xin-Er-Hao, Dan-Chuan-Hong or normal saline at 30 minutes before ischemia. The model of acute myocardial infarction (AMI) in rats was set up by ligating the descending branch of left coronary artery. 3 hours after AMI, the hearts were isolated to detect the apoptosis of myocardial ischemia by TUNEL; 24 hours after AMI, the hearts were isolated to determine the area of myocardial infarction by quantitative histological assay (triphenyltetrazolium chloride, TTC stain).


RESULTS
Comparing with model group,the myocardial infarct size and apoptosis index of all the treatment groups decreased. Comparing with Guan-Xin-Er-Hao (30 g/kg) or Dan-Chuan-Hong (10 g/kg), the myocardial infarct size and apoptosis index of Dan-Chuan-Hong (20 g/kg) had no statistic difference. And there was statistic difference between Guan-Xin-Er-Hao (30 g/kg)and Dan-Chuan-Hong (10 g/kg).


CONCLUSIONS
Comparing with Guan-Xin-Er-Hao, the decoction of Dan-Chuan-Hong has similar effect, which could protect hearts from myocardial ischemia by inhibiting the myocardial apoptosis.
Wang
Xiao-Yu
XY
Laboratory of Ethnopharmacology and Department of Integrated Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. pucca1029@qq.com
Qin
Feng
F
Huang
Xi
X
Zhang
Xue-Ya
XY
Ren
Ping
P
Zhao
Hong-Wei
HW
D000208:Acute Disease; D000818:Animals; D019661:Apiaceae / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects*; D031187:Carthamus tinctorius / Q000737:chemistry; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy / Q000473:pathology* / Q000517:prevention & control; D017202:Myocardial Ischemia / Q000188:drug therapy / Q000473:pathology* / Q000517:prevention & control; D009206:Myocardium / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C534196:guan xin II