title: [Operator-constitutive mutation in the recA gene enhances radiation resistance of Escherichia coli].
abstract: Plasmid pUC19-recAoc carrying a mutant allele of the recA gene, which plays the key role in the control of the SOS repair system and homologous recombinational repair, causes a 1.5-fold increase in radiation resistance of Escherichia coli DeltarecA cells, as compared to the wild-type recA+ cells. The protective effect of this plasmid is drastically reduced in mutant lexA3 recADelta21 deficient in the LexA protein and in induction of the SOS regulon. Plasmid pUC19-recAoc effectively suppresses UV sensitivity of the DeltarecA mutant. Mutation recAo20 allows constitutive high-level synthesis of the RecA protein. This mutation impairs the SOS box in the operator site of the recA gene and enhances heterology of the dimer LexA binding site. These data confirm that high level of the RecA protein synthesis per se is not sufficient for the expression of gamma-inducible functions and that the derepression of lexA-dependent genes, other than recA gene, is necessary for the complete induction of the SOS repair system.
authors.forename: V N
authors.initials: VN
authors.lastname: Verbenko
authors.forename: L V
authors.initials: LV
authors.lastname: Kuznetsova
authors.forename: E P
authors.initials: EP
authors.lastname: Krup'ian
authors.forename: A V
authors.initials: AV
authors.lastname: Suslov
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005720:Gamma Rays; D009154:Mutation; D009875:Operator Regions, Genetic; D011836:Radiation Tolerance / Q000235:genetics* / Q000528:radiation effects; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D013014:SOS Response, Genetics / Q000502:physiology / Q000528:radiation effects; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein