Reversible effect of magnetic fields on human lymphocyte activation patterns: different sensitivity of naive and memory lymphocyte subsets.
The aim of this study was to investigate the influence of 50 Hz magnetic or static magnetic fields of 0.5 mT on subsets of human CD4(+) T cells in terms of cytokine release/content, cell proliferation and intracellular free calcium concentration. CD4(+) T cells can be divided into different subsets on the basis of surface marker expression, such as CD45, and T cells can be divided into naive (CD45RA(+)) and memory (CD45RA(-)) cells. In this study, the effects of magnetic fields after 24 and 48 h of cell culture were analyzed. We found that the CD4(+)CD45RA(-) T subset were more sensitive after 2 h of exposure. Decreases in the release/content of IFN-gamma, in cell proliferation and in intracellular free calcium concentrations were observed in exposed CD4(+)CD45RA(-) T cells compared to CD4(+)CD45RA(+) T cells. The results suggest that exposure to the magnetic fields induces a delay in the response to stimulants and that modifications are rapidly reversible, at least after a short exposure.
Salerno
Sergio
S
Dipartimento di Biotecnologie Mediche e Medicina Legale, Università di Palermo, Palermo, Italy.
La Mendola
Carmela
C
La Manna
Marco Pio
MP
Lo Casto
Antonio
A
Caccamo
Nadia
N
Salerno
Alfredo
A
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology* / Q000528:radiation effects; D007371:Interferon-gamma / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation / Q000528:radiation effects; D008280:Magnetics; D008297:Male; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors
D007371:Interferon-gamma; D017493:Leukocyte Common Antigens; D002118:Calcium