title: Effect of H/D isotopomerization in the assay of resveratrol by tandem mass spectrometry and isotope dilution method.
abstract: Resveratrol is a phytoalexin found in several plant tissues and present in wines, which is supplied as a nutritional supplement. Different studies have revealed its beneficial effects as anticancer, antiviral, neuroprotective, antiaging, and anti-inflammatory natural active principle. The assaying of resveratrol by mass spectrometry and isotope dilution method, using a stable [(2)H(4)] analogue, has required a full elucidation of its gas-phase H/D isotopomerization. Either selected ion monitoring (SIM) or multiple reaction monitoring (MRM) methods have been used for the evaluation of the amount of resveratrol present in wine and plasma samples in the negative ionization mode. In all instances the acquired accuracy, limit of quantitation (LOQ), and limit of detection (LOD) are fit for the intended purpose of the assay.
authors.affiliation: Dipartimento di Chimica, Universita della Calabria, Via P. Bucci, Cubo 12/C, I-87030 Arcavacata di Rende (CS)-Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Hicham
authors.initials: H
authors.lastname: Benabdelkamel
authors.forename: Bartolo
authors.initials: B
authors.lastname: Gabriele
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Plastina
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D006801:Humans; D007536:Isomerism; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis* / Q000097:blood / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014920:Wine / Q000032:analysis
chemical_list: D013267:Stilbenes; D003903:Deuterium; D000077185:Resveratrol