Carbon nanotube-hydroxyapatite-hemoglobin nanocomposites with high bioelectrocatalytic activity.
In this paper, carbon nanotubes-hydroxyapatite (MWCNTs-HA) composite were synthesized by self-assembling technique, and investigated by X-ray diffraction, scanning electron microscopy (SEM) and FT-IR spectroscopy. Hemoglobin (Hb) immobilized in MWCNTs-HA film not only retained its similarly native conformations, but also achieved the direct electron transfer. The apparent heterogeneous electron transfer rate constant (k(s)) was evaluated as (5.05+/-0.41) s(-1) according to Laviron's equation, and the surface coverage (Gamma*) was estimated as (9.25+/-0.69)x10(-10)mol cm(-2). Moreover, the Hb immobilized in MWCNTs-HA film exhibited remarkable bioelectrocatalytic activity for the electrochemical reduction of H(2)O(2). The amperometric response of the biosensor varied linearly with the H(2)O(2) concentration ranging from 0.5microM to 2microM, and the results showed a detection limit of 0.09microM. Furthermore, the Hb-immobilized MWCNTs-HA film exhibited the excellent catalytic reactivity toward trichloroacetic acid (TCA).
Zhao
H Y
HY
Center for Biomedical Materials and Engineering, Harbin Engineering University, Nan-Gang District, Harbin 150001, China.
Xu
X X
XX
Zhang
J X
JX
Zheng
W
W
Zheng
Y F
YF
D000818:Animals; D002384:Catalysis; D002417:Cattle; D017886:Durapatite / Q000737:chemistry*; D004563:Electrochemistry; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011487:Protein Conformation; D014238:Trichloroacetic Acid / Q000378:metabolism
D006454:Hemoglobins; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D014238:Trichloroacetic Acid; D017886:Durapatite; D006861:Hydrogen Peroxide