Design of extremely facile 3'- and 5'- ends free molecular beacons using C8 alkylamino substituted 2'-deoxyguanosine.
A novel fluorescent oligonucleotide probe containing pyrene-labeled C8 alkylamino-substituted 2'-deoxyguanosine and a practically useful 3'- and 5'- ends-free self-quenched molecular beacons (MB) were designed. A unique MB detectable by pyrene excimer fluorescence was also demonstrated.
Matsumoto
Katsuhiko
K
Department of Materials Chemistry and Engineering and NEWCAT Institute, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
Shinohara
Yuta
Y
Numajiri
Kyoko
K
Ishioroshi
Shinya
S
Morii
Takashi
T
Saito
Yoshio
Y
Saito
Isao
I
D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D003849:Deoxyguanosine