title: Solution structure of the E. coli ribosome hibernation promoting factor HPF: Implications for the relationship between structure and function.
abstract: The 70S Escherichia coli ribosome dimerizes to form an inactive 100S ribosome during stationary phase, which is called "ribosome hibernation". The hibernation promoting factor HPF plays a crucial role in 100S ribosome formation. However, YfiA, a known paralog of HPF inhibits 100S formation, although it shares high sequence similarity. Here, we report the first solution structure of HPF as determined by multi-dimensional NMR. HPF adopts betaalphabetabetabetaalpha-fold and the overall structure is similar to YfiA as expected. However, detailed structure comparison based on the determined structure in this study revealed that there are remarkable differences around the C-terminal portion of helix alpha2, which is not predicted by homology modeling. Furthermore, some acidic residues conserved only in HPF are located at the rim of the common basic patch.
authors.affiliation: Graduate School of Science and Technology, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Sato
authors.forename: Takumi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Maki
authors.forename: Masami
authors.initials: M
authors.lastname: Ueta
authors.forename: Hideji
authors.initials: H
authors.lastname: Yoshida
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ito
authors.forename: Akira
authors.initials: A
authors.lastname: Wada
authors.forename: Masaki
authors.initials: M
authors.lastname: Mishima
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D029968:Escherichia coli Proteins; C422854:HPF protein, E coli; D012269:Ribosomal Proteins