title: Two novel cSNPs of weaver gene in Chinese indigenous goat and their associations with milk yield.
abstract: Weaver gene plays an essential physiological role in the function of many organs, including brain, heart, kidney and endocrine cells, and also in the regulation of insulin secretion by glucose and/or neurotransmitters. Thus, weaver gene is an important potential candidate gene effecting on performance traits. The objective of this study was to detect the genetic variation of five loci within weaver gene by PCR-SSCP, DNA sequencing and forced PCR-RFLP methods in 1,019 Chinese indigenous goats. Two novel coding SNPs (XM_598993:m.864G>A; XM_598993:m.1224T>A) locating on P3 and P4 loci were identified and detected by MluI and AsuII forced PCR-RFLP, respectively. In the MluI analysis, the frequencies of goat MluI-A allele in the analyzed populations were 0.226, 0.248, 0.096 and 0.088 for XNSN, GZ, SBWC and XJWC, respectively. Genotypic frequencies were found to be significantly different in four breeds (chi2 = 75.842, df = 6, P < 0.001); In the AsuII analysis, the frequencies of goat AsuII-A allele in the analyzed populations were 0.584, 0.441, 0.073 and 0.063 for XNSN, GZ, SBWC and XJWC, respectively. Genotypic frequencies were found to be significantly different among four breeds (chi2 = 399.464, df = 6, P < 0.001). The frequencies of allele MluI-A and AsuII-A in XNSN and GZ populations were significantly higher than those of SBWC and XJWC goats. Association analysis with adjusted milk yield in the XNSN breed indicated that the animals with AsuII-AA genotype owned significantly higher adjusted milk yield than the ones with AsuII-TT genotype in the second lactation (P < 0.05). The observation suggested that the allele "AsuII-A" had the positive effects on adjusted milk yield in the second lactation.
authors.affiliation: College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, People's Republic of China. lizhuanjian@yahoo.com.cn
authors.forename: Zhuanjian
authors.initials: Z
authors.lastname: Li
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Chen
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Qu
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.forename: Mijie
authors.initials: M
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002681:China; D004587:Electrophoresis, Agar Gel; D005260:Female; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D006041:Goats / Q000235:genetics*; D007774:Lactation / Q000235:genetics*; D008892:Milk / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels