title: Evaluation of mobility of 2-N-tert-butylaminoxyladenosine incorporated into oligodeoxynucleotides.
abstract: We synthesized oligodeoxynucleotide (ODN, 3) containing 2-N-tert-butylaminoxyladenosine (1) and studied EPR spectra of 3 and its duplexes. The h(+)/h(0) values in the EPR spectra of duplexes between 3 and 5-8 well correlated with Tm values. We also synthesized ODN 4 containing 2, which has a cyclic aminoxyl via a linker, to compare with ODN 3. The h(+)/h(0) values in the EPR spectra of duplexes between 4 and 5-8 did not correlate with Tm values. These results indicate that 1 has a potential to monitor of motion of the nucleobase.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Mariko
authors.initials: M
authors.lastname: Aso
authors.forename: Ryouko
authors.initials: R
authors.lastname: Harada
authors.forename: Noboru
authors.initials: N
authors.lastname: Koga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemune
mesh_terms: D000241:Adenosine / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D009038:Motion; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D009838:Oligodeoxyribonucleotides; D013113:Spin Labels; D000241:Adenosine