title: CD133 gene overexpression is frequently observed in early colorectal carcinoma.
abstract: OBJECTIVE
  Recently, it has been proved that colorectal carcinoma is created and propagated by a small number of undifferentiated tumorigenic CD133+ cells. The present study examined the CD133 gene expression in colorectal carcinomas we surgically removed.
  
  
  METHODS
  The CD133 gene expression in primary tumors and corresponding normal tissues derived from 30 patients with colorectal carcinoma was examined using a quantitative reverse-transcription PCR (QRT-PCR) and evaluated the correlation between the CD133 gene expression levels and the clinicopathological findings.
  
  
  RESULTS
  An increase in CD133 expression scores was observed in Dukes A's colorectal carcinomas (1.70 +/- 2.28) compared to Dukes B and C's colorectal carcinomas (0.76 +/- 0.71) (p = 0.0860). An increase in CD133 expression scores was also observed in female colorectal carcinomas (1.29 +/- 1.56) compared to male colorectal carcinomas (0.61 +/- 0.57) (p = 0.125).
  
  
  CONCLUSIONS
  CD133 gene overexpression in early colorectal cancer patients was more frequently observed than in advanced cases.
authors.affiliation: Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama 227-8501, Japan. kenjih-ngy@umin.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Hibi
authors.forename: Makiko
authors.initials: M
authors.lastname: Sakata
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sakuraba
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shirahata
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Goto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizukami
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Saito
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Gaku
authors.initials: G
authors.lastname: Kigawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nemoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sanada
mesh_terms: D000071916:AC133 Antigen; D000368:Aged; D015703:Antigens, CD / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008297:Male; D010455:Peptides / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides