title: In vitro and in vivo evaluation of the effects of demineralized bone  matrix or calcium sulfate addition to polycaprolactone-bioglass  composites.
abstract: The objective of this study was to improve the efficacy of polycaprolactone/bioglass (PCL/BG) bone substitute using demineralized bone matrix (DBM) or calcium sulfate (CS) as a third component. Composite discs involving either DBM or CS were prepared by compression moulding. Bioactivity of discs was evaluated by energy dispersive X-ray spectroscopy (ESCA) and scanning electron microscopy (SEM) following simulated body fluid incubation. The closest Calcium/Phosphate ratio to that of hydroxyl carbonate apatite crystals was observed for PCL/ BG/DBM group (1.53) after 15 day incubation. Addition of fillers increased microhardness and compressive modulus of discs. However, after 4 and 6-week PBS incubations, PCL/BG/DBM discs showed significant decrease in modulus (from 266.23 to 54.04 and 33.45 MPa, respectively) in parallel with its highest water uptakes (36.3 and 34.7%). Discs preserved their integrity with only considerable weight loss (7.5-14.5%) in PCL/BG/DBM group. In vitro cytotoxicity tests showed that all discs were biocompatible.
authors.affiliation: Department of Engineering Sciences, Middle East Technical University, 06531 Ankara, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Erdemli
authors.forename: O
authors.initials: O
authors.lastname: Captug
authors.forename: H
authors.initials: H
authors.lastname: Bilgili
authors.forename: D
authors.initials: D
authors.lastname: Orhan
authors.forename: A
authors.initials: A
authors.lastname: Tezcaner
authors.forename: D
authors.initials: D
authors.lastname: Keskin
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D018775:Bone Demineralization Technique; D015519:Bone Density / Q000502:physiology; D001857:Bone Matrix / Q000737:chemistry / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry; D002133:Calcium Sulfate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D006245:Hardness Tests; D006801:Humans; D008297:Male; D008954:Models, Biological; D053761:Nanocomposites / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D013499:Surface Properties
chemical_list: C022107:Bioglass; D018786:Bone Substitutes; D011091:Polyesters; C016240:polycaprolactone; D002133:Calcium Sulfate