title: Influence of internal capsid pressure on viral infection by phage lambda.
abstract: Ejection of the genome from the virus, phage lambda, is the initial step in the infection of its host bacterium. In vitro, the ejection depends sensitively on internal pressure within the virus capsid; however, the in vivo effect of internal pressure on infection of bacteria is unknown. Here, we use microfluidics to monitor individual cells and determine the temporal distribution of lysis due to infection as the capsid pressure is varied. The lysis probability decreases markedly with decreased capsid pressure. Of interest, the average lysis times remain the same but the distribution is broadened as the pressure is lowered.
authors.affiliation: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Köster
authors.forename: Alex
authors.initials: A
authors.lastname: Evilevitch
authors.forename: Meerim
authors.initials: M
authors.lastname: Jeembaeva
authors.forename: David A
authors.initials: DA
authors.lastname: Weitz
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002213:Capsid / Q000378:metabolism*; D016923:Cell Death; D049109:Cell Proliferation; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000821:virology; D016679:Genome, Viral / Q000235:genetics; D011312:Pressure; D011336:Probability; D012492:Salts / Q000494:pharmacology; D013997:Time Factors
chemical_list: D004279:DNA, Viral; D012492:Salts