Cloning and characterization of a wheat beta-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici.
b-1,3-Glucanases are a group of pathogenesis related proteins that have been reported to be involved in plant defense against pathogens in many other plant pathogen systems. However, it was not clear if these genes play similar role in wheat (Triticum aestivum L.) against Puccinia striiformis f. sp. tritici (Pst), the stripe rust pathogen. To investigate the role of b-1,3-glucanase (EC3.2.1.39) in the resistance response of wheat (cv. Suwon11) to stripe rust, a wheat b-1,3-glucanase gene induced by Pst, designated as TaGlu, was cloned and characterized.TaGlu was predicted to encode a basic protein of 334 amino acids. Quantitative real-time PCR analyses revealed that the transcription of TaGlu was induced during both compatible and incompatible interactions with Pst, but the transcription level was much higher in the incompatible interaction than that in the compatible interaction. TaGlu also showed noticeable induction of gene expression in young green leaf tissues treated with salicylic acid, methyl jasmonate or ethylene. Immunogold labeling assays showed that the enzyme were localized mainly in the host cell wall and over the extra haustorial matrix, and the labeling densities were found significantly higher in the incompatible interaction than those in the compatible interaction.
Liu
Bo
B
College of Plant Protection, Northwest A&F University, 712100 Yangling, Shaanxi, People's Republic of China.
Xue
Xiaodan
X
Cui
Suping
S
Zhang
Xiaoyu
X
Han
Qingmei
Q
Zhu
Lin
L
Liang
Xiaofei
X
Wang
Xiaojie
X
Huang
Lili
L
Chen
Xianming
X
Kang
Zhensheng
Z
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000276:immunology / Q000502:physiology*; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D010940:Plant Proteins / Q000235:genetics; D014908:Triticum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; D043326:Glucan 1,3-beta-Glucosidase