title: Gene expression changes in medical workers exposed to radiation.
abstract: The use of nuclear resources for medical purposes causes considerable concern about occupational exposure. Nevertheless, little information is available regarding the effects of low-dose irradiations protracted over time. We used oligomicroarrays to identify the genes that are transcriptionally regulated by persistent exposure to extremely low doses of ionizing radiation in 28 exposed professionals (mean cumulative effective dose +/- SD, 19 +/- 38 mSv) compared with a matched sample of nonexposed subjects. We identified 256 modulated genes from peripheral blood mononuclear cells profiles, and the main biological processes we found were DNA packaging and mitochondrial electron transport NADH to ubiquinone. Next we investigated whether a different pattern existed when only 22 exposed subjects with accumulated doses >2.5 mSv, a threshold corresponding to the natural background radiation in Italy per year, and mean equal to 25 +/- 41 mSv were used. In addition to DNA packaging and NADH dehydrogenase function, the analysis of the higher-exposed subgroup revealed a significant modulation of ion homeostasis and programmed cell death as well. The changes in gene expression that we found suggest different mechanisms from those involved in high-dose studies that may help to define new biomarkers of radiation exposure for accumulated doses below 25 mSv.
authors.affiliation: Environmental Carcinogenesis and Risk Assessment, Environmental Protection and Health Prevention Agency-Emilia-Romagna Region (ER-EPA), 40126, Bologna County, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Morandi
authors.forename: Cinzia
authors.initials: C
authors.lastname: Severini
authors.forename: Daniele
authors.initials: D
authors.lastname: Quercioli
authors.forename: Stefania
authors.initials: S
authors.lastname: Perdichizzi
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Mascolo
authors.forename: Wolfango
authors.initials: W
authors.lastname: Horn
authors.forename: Monica
authors.initials: M
authors.lastname: Vaccari
authors.forename: Maria Concetta
authors.initials: MC
authors.lastname: Nucci
authors.forename: Vittorio
authors.initials: V
authors.lastname: Lodi
authors.forename: Francesco Saverio
authors.initials: FS
authors.lastname: Violante
authors.forename: Claudia
authors.initials: C
authors.lastname: Bolognesi
authors.forename: Sandro
authors.initials: S
authors.lastname: Grilli
authors.forename: Paola
authors.initials: P
authors.lastname: Silingardi
authors.forename: Annamaria
authors.initials: A
authors.lastname: Colacci
mesh_terms: D000328:Adult; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006282:Health Personnel; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D020411:Oligonucleotide Array Sequence Analysis; D011829:Radiation Dosage; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment