title: Pr-1, a novel antifungal protein from pumpkin rinds.
abstract: A novel antifungal protein, M(r) = ca. 40 kDa, was isolated from pumpkin rind and designated Pr-1. When purified by anion exchange chromatography and HPLC, it inhibited growth of several fungi including Botrytis cinerea, Fusarium oxysporum, Fusarium solani and Rhizoctonia solani, as well as the yeast, Candida albicans, at 10-20 microM. It did not inhibit growth of Escherichia coli or Staphylococcus aureus even at 200 microM. Laser scanning microscopy of fungal cells exposed to rhodamine-labeled Pr-1 revealed that the protein accumulated and was localized on the cell surface. Uptake of the vital stain, SYTOX Green, was enhanced when fungal conidia were treated with Pr-1 suggesting that the protein has membrane permeabilization activity. Pr-1 was thermostable at 70 degrees C and did not lyse human red blood cells at 128 microM suggesting that the protein may be useful as an antifungal agent with little, if any human cytotoxicity.
authors.affiliation: Research Center for Proteineous Materials (RCPM), Chosun University, Kwangju, Korea.
authors.forename: Seong-Cheol
authors.initials: SC
authors.lastname: Park
authors.forename: Jung Ro
authors.initials: JR
authors.lastname: Lee
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Indeok
authors.initials: I
authors.lastname: Hwang
authors.forename: Jae-Woon
authors.initials: JW
authors.lastname: Nah
authors.forename: Hyeonsook
authors.initials: H
authors.lastname: Cheong
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D020171:Botrytis / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D028464:Cucurbita / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D055550:Protein Stability; D012232:Rhizoctonia / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D010940:Plant Proteins