A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors.
BACKGROUND
Stable transgenesis is an undeniable key to understanding any genetic system. Retrovirus-based insertional strategies, which feature several technical challenges when they are used, are often limited to one particular species, and even sometimes to a particular cell type as the infection depends on certain cellular receptors. A universal-like system, which would allow both stable transgene expression independent of the cell type and an efficient sorting of transfected cells, is required when handling cellular models that are incompatible with retroviral strategies.


RESULTS
We report here on the combination of a stable insertional transgenesis technique, based on the Tol2 transposon system together with the magnetic cell sorting (MACS) technique, which allows specific selection of cells carrying the transgene in an efficient, reliable and rapid way.


CONCLUSIONS
This new Tol2/MACS system leads to stable expression in a culture of primary chicken erythroid cells highly enriched in cells expressing the transgene of interest. This system could be used in a wide variety of vertebrate species.
Mejia-Pous
Camila
C
Equipe Bases Moléculaires de l'Autorenouvellement et de ses Altérations, Université de Lyon, Villeurbanne, Lyon, France. Camila.Mejia@cgmc.univ-lyon1.fr
Viñuelas
José
J
Faure
Claudine
C
Koszela
Joanna
J
Kawakami
Koichi
K
Takahashi
Yoshiko
Y
Gandrillon
Olivier
O
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D002645:Chickens; D004251:DNA Transposable Elements; D015672:Erythroid Precursor Cells; D016254:Mutagenesis, Insertional; D010957:Plasmids; D014162:Transfection / Q000379:methods*; D019076:Transgenes
D004251:DNA Transposable Elements