title: [Effects of tumor necrosis factor-alpha on the growth of rat osteoblasts].
abstract: OBJECTIVE
  To investigate the effects of tumor necrosis factor alpha (TNF-alpha) on the growth of rat osteoblasts. To find out the mechanisms that TNF-alpha regulates the growth of osteoblasts.
  
  
  METHODS
  To assay osteoblasts proliferation by MTT. To assay alkaline phosphatase (ALP) activity of osteoblasts by PP-nitrophenyl phosphate (PNPP).
  
  
  RESULTS
  The osteblasts proliferation and the ALP activity decreased in treatment groups, and the significantly lower levels were observed in above 50 ng/mL groups (P<0.05).
  
  
  CONCLUSIONS
  TNF-alpha restrained osteoblasts proliferation and differentiation, and the effects were more significant in above 50 ng/mL groups.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan 250012, China.
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Xue
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Xu-xia
authors.initials: XX
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Bu
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D010006:Osteoblasts; D051381:Rats; D014409:Tumor Necrosis Factor-alpha
chemical_list: D014409:Tumor Necrosis Factor-alpha; D000469:Alkaline Phosphatase