title: Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer.
abstract: We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.
authors.affiliation: Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge CB2 1QR, UK.
authors.forename: Michaela
authors.initials: M
authors.lastname: Frye
authors.forename: Ilaria
authors.initials: I
authors.lastname: Dragoni
authors.forename: Suet-Feung
authors.initials: SF
authors.lastname: Chin
authors.forename: Immaculada
authors.initials: I
authors.lastname: Spiteri
authors.forename: Agata
authors.initials: A
authors.lastname: Kurowski
authors.forename: Elena
authors.initials: E
authors.lastname: Provenzano
authors.forename: Andrew
authors.initials: A
authors.lastname: Green
authors.forename: Ian O
authors.initials: IO
authors.lastname: Ellis
authors.forename: Donna
authors.initials: D
authors.lastname: Grimmer
authors.forename: Andrew
authors.initials: A
authors.lastname: Teschendorff
authors.forename: Christos C
authors.initials: CC
authors.lastname: Zouboulis
authors.forename: Carlos
authors.initials: C
authors.lastname: Caldas
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Watt
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics*; D002470:Cell Survival; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D008780:Methyltransferases; C515610:NSUN2 protein, human