title: Marine compound Xyloketal B protects PC12 cells against OGD-induced cell damage.
abstract: Xyloketal B is a novel marine compound with unique chemical structure isolated from mangrove fungus Xylaria sp. (no. 2508). Recently, we have demonstrated that Xyloketal B is an antioxidant and can protect against oxidized low density lipoprotein (LDL)-induced cell injury. In the present study, we investigated whether Xyloketal B can protect against ischemia-induced cell injury in an in vitro oxygen glucose deprivation (OGD) model of ischemic stroke in PC12 cells. We found that Xyloketal B could directly scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and protect PC12 cells against OGD insult. Furthermore, Xyloketal B alleviated OGD-induced mitochondria superoxide, mitochondria fragmentation and GTPase dynamin-related protein 1 (Drp1) overexpression as well as reduction of mitochondrial membrane potential. All together, the present study demonstrates that Xyloketal B protects PC12 cells against OGD-induced cell injury and that the anti-oxidative property and protective action on mitochondria may account for its neuroprotective actions.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Ling
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Ji-Yan
authors.initials: JY
authors.lastname: Pang
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Lin
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Ruxun
authors.initials: R
authors.lastname: Huang
authors.forename: Guan-Lei
authors.initials: GL
authors.lastname: Wang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Pei
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use; D005609:Free Radicals / Q000037:antagonists & inhibitors / Q000378:metabolism; D008954:Models, Biological; D009410:Nerve Degeneration / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D011714:Pyrans / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D020521:Stroke / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D018696:Neuroprotective Agents; D011714:Pyrans; C515343:xyloketal B