Quantitative miRNA expression analysis: comparing microarrays with next-generation sequencing.
Recently, next-generation sequencing has been introduced as a promising, new platform for assessing the copy number of transcripts, while the existing microarray technology is considered less reliable for absolute, quantitative expression measurements. Nonetheless, so far, results from the two technologies have only been compared based on biological data, leading to the conclusion that, although they are somewhat correlated, expression values differ significantly. Here, we use synthetic RNA samples, resembling human microRNA samples, to find that microarray expression measures actually correlate better with sample RNA content than expression measures obtained from sequencing data. In addition, microarrays appear highly sensitive and perform equivalently to next-generation sequencing in terms of reproducibility and relative ratio quantification.
Willenbrock
Hanni
H
Exiqon, DK-2950 Vedbaek, Denmark. haw@exiqon.com
Salomon
Jesper
J
Søkilde
Rolf
R
Barken
Kim Bundvig
KB
Hansen
Thomas Nøhr
TN
Nielsen
Finn Cilius
FC
Møller
Søren
S
Litman
Thomas
T
D015870:Gene Expression; D035683:MicroRNAs / Q000032:analysis* / Q000138:chemical synthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA / Q000379:methods*
D035683:MicroRNAs