Genistein inhibition of topoisomerase IIalpha expression participated by Sp1 and Sp3 in HeLa cell.
Genistein (4', 5, 7-trihydroxyisoflavone) is an isoflavone compound obtained from plants that has potential applications in cancer therapy. However, the molecular mechanism of the action of genistein on cancer cell apoptosis is not well known. In this study, we investigated the effect of genistein on topoisomerase II-alpha (Topo IIalpha), an important protein involved in the processes of DNA replication and cell proliferation. The results revealed that inhibition of Topo IIalpha expression through the regulation of Specificity protein 1 and Specificity protein 3 may be one of the reasons for genistein's induction of HeLa cell apoptosis.
Zhou
Najing
N
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Yan
Yunli
Y
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Li
Wenling
W
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Wang
Yanling
Y
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Zheng
Lifen
L
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Han
Shuo
S
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Yan
Yongxin
Y
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Li
Yunzhi
Y
Chang'an District Hospital, Shijiazhuang 050017, Hebei, China.
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D019833:Genistein / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D051705:Sp3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D016588:Anticarcinogenic Agents; D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D051705:Sp3 Transcription Factor; D019833:Genistein; D004250:DNA Topoisomerases, Type II