A determination of submicromolar concentrations of glycine in periaqueductal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection.
CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 microM by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
Tůma
Petr
P
Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Czech Republic. petr.tuma@lf3.cuni.cz
Soukupová
Marie
M
Samcová
Eva
E
Stulík
Karel
K
D000082:Acetaminophen / Q000494:pharmacology; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D005998:Glycine / Q000032:analysis* / Q000378:metabolism; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D010487:Periaqueductal Gray / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen; D002351:Carrageenan; D005998:Glycine