title: Enhancement of angiogenic and vasculogenic potential of endothelial progenitor cells by haptoglobin.
abstract: Endothelial progenitor cells (EPCs) were transfected with the haptoglobin (Hp) gene to investigate the effect of Hp on cell function. Hp potentiated the gene expression of various pro-angiogenic factors in the EPCs. The Hp-modified EPCs also increased in vitro tube formation on Matrigel compared with control cells. In hindlimb ischaemia models, Hp-EPCs showed a greater ability for improving blood perfusion and recovery from ischaemic injury. These results indicate that Hp improves EPC function in neovasculogenesis, which suggests that ex vivo modification of EPCs with the Hp gene can be applied to the treatment of vascular damage.
authors.affiliation: Department of Natural Sciences, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of Korea.
authors.forename: Seon-Joo
authors.initials: SJ
authors.lastname: Park
authors.forename: Sang Hong
authors.initials: SH
authors.lastname: Baek
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Oh
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Choi
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Park
authors.forename: Nam-Hoon
authors.initials: NH
authors.lastname: Kim
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Shin
authors.forename: In-Sook
authors.initials: IS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D042783:Endothelial Cells / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology; D006242:Haptoglobins / Q000235:genetics / Q000502:physiology*; D006614:Hindlimb / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000503:physiopathology; D051379:Mice; D018919:Neovascularization, Physiologic / Q000235:genetics*; D013234:Stem Cells / Q000502:physiology*; D014162:Transfection
chemical_list: D006242:Haptoglobins