Distinctive profiles of infection and pathology in hamsters infected with Clostridium difficile strains 630 and B1.
Currently, the Golden Syrian hamster is widely considered an important model of Clostridium difficile disease, as oral infection of this animal pretreated with antibiotics reproduces many of the symptoms observed in humans. Two C. difficile strains, B1 and 630, showed significant differences in the progression and severity of disease in this model. B1-infected hamsters exhibited more severe pathology and a shorter time to death than hamsters infected with 630. Histological changes in the gut did not correlate with absolute numbers of C. difficile bacteria, but there were clear differences in the distribution of bacteria within gut tissues. Light, scanning, and transmission electron microscopy revealed high numbers of B1 bacteria at the mucosal surface of the tissue, whereas 630 bacteria were more frequently associated with the crypt regions. Both B1 and 630 bacteria were frequently observed within polymorphonuclear leukocytes, although, interestingly, a space frequently separated B1 bacteria from the phagosome wall, a phenomenon not observed with 630. However, pilus-like structures were detected on 630 located in the crypts of the gut tissue. Furthermore, B1 bacteria, but not 630 bacteria, were found within nonphagocytic cells, including enterocytes and muscle cells.
Goulding
David
D
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.
Thompson
Harold
H
Emerson
Jenny
J
Fairweather
Neil F
NF
Dougan
Gordon
G
Douce
Gill R
GR
D000818:Animals; D016360:Clostridioides difficile / Q000472:pathogenicity*; D003015:Clostridium Infections / Q000473:pathology* / Q000503:physiopathology*; D006224:Cricetinae; D004760:Enterocolitis / Q000382:microbiology / Q000473:pathology* / Q000503:physiopathology*; D020895:Enterocytes / Q000382:microbiology; D005260:Female; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D041981:Gastrointestinal Tract / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000382:microbiology / Q000648:ultrastructure; D008647:Mesocricetus; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D032342:Muscle Cells / Q000382:microbiology; D009504:Neutrophils / Q000382:microbiology; D010588:Phagosomes / Q000382:microbiology; D012720:Severity of Illness Index; D016019:Survival Analysis