SNP detection using trityl mass tags.
A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption-ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.
Birikh
Klara R
KR
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya, Moscow, Russia.
Bernad
Pablo L
PL
Shmanai
Vadim V
VV
Malakhov
Andrei D
AD
Shchepinov
Mikhail S
MS
Korshun
Vladimir A
VA
D015342:DNA Probes / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D009841:Oligonucleotides / Q000138:chemical synthesis; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014320:Triphenylmethyl Compounds / Q000737:chemistry*
D015342:DNA Probes; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D014320:Triphenylmethyl Compounds; C434331:phosphoramidite