A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose.
A biosensor for the measurement of glucose in serum has been developed, based on a screen-printed carbon electrode modified with Meldola's Blue-Reinecke salt, coated with the enzyme glucose dehydrogenase (from Bacillus sp.), and nicotinamide adenine dinucleotide coenzyme (NAD+). A cellulose acetate layer was deposited on top of the device to act as a permselective membrane. The biosensor was incorporated into a commercially available, thin-layer, amperometric flow cell operated at a potential of only +0.05 V versus Ag/AgCl. The mobile phase consisted of 0.2 M phosphate buffer (pH 7.0) containing 0.1 M potassium chloride solution, and a flow rate of 0.8 ml min(-1) was used throughout the investigation. The biosensor response was linear over the range of 0.075-30 mM glucose, with the former representing the detection limit. The precision of the system was determined by carrying out 20 repeat injections of a 5-mM glucose standard, and the calculated coefficient of variation was 3.9%. It was demonstrated that this biosensor system could be applied to the direct measurement of glucose in serum without pretreatment. Therefore, this would allow high-throughput analysis, at low cost, for this clinically important analyte.
Piano
M
M
Applied Enzyme Technology, Monmouth House, Mamhilad Park, Pontypool NP46HZ, UK.
Serban
S
S
Biddle
N
N
Pittson
R
R
Drago
G A
GA
Hart
J P
JP
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D002244:Carbon / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D017022:Flow Injection Analysis / Q000379:methods*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods; D057230:Limit of Detection; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry*; D013861:Thiocyanates / Q000737:chemistry*
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D010078:Oxazines; D013861:Thiocyanates; D009243:NAD; C001818:Reinecke Salt; C005062:acetylcellulose; C005559:Meldola blue; D002244:Carbon; D002482:Cellulose; D042843:Glucose 1-Dehydrogenase