Production of high level of cellulase-poor xylanases by wild strains of white-rot fungus Coprinellus disseminatus in solid-state fermentation.
The production, optimisation and partial characterisation of xylanases from newly isolated wild strains of Coprinellus disseminatus was performed in solid-state fermentation. Strains SH-1 and SH-2 showed high xylanase (727.78 and 227.99 IU/mL) with very low CMCase (0.925 and 0.660 IU/mL) and laccase (0.640 and 0.742 U/mL) activities at incubation time seven days, 37 degrees C and initial pH 6.4, using yeast extract as nitrogen source and cheap substrate (wheat bran), which increased the cost effectiveness of the process. Crude cellulase-poor xylanases obtained from test strains showed maximum activities at 55 degrees C and pH 6.4 and retained 32.64 (SH-1) and 35.03% (SH-2) activity at pH 8 and 43.01 (SH-1) and 25.00% (SH-2) activity at 65 degrees C. As test strains produced high level of cellulase-poor xylanases, which were active over a wide range of temperature and pH, these enzymes might be used as pulp biobleaching agents.
Singh
Shalini
S
Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Roorkee, India. shalinisingh.iit@gmail.com
Tyagi
C H
CH
Dutt
Dharm
D
Upadhyaya
J S
JS
D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007785:Lactose / Q000494:pharmacology; D008031:Lignin / Q000378:metabolism; D009584:Nitrogen / Q000494:pharmacology; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D013696:Temperature; D013997:Time Factors
D005656:Fungal Proteins; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D005947:Glucose; D007785:Lactose; D009584:Nitrogen