title: Features of structural organization and expression regulation of malate dehydrogenase isoforms from Rhodobacter sphaeroides strain 2R.
abstract: Two isoforms of malate dehydrogenase (MDH), dimeric and tetrameric, have been found in the purple non-sulfur bacterium Rhodobacter sphaeroides strain 2R, devoid of the glyoxylate shunt, which assimilate acetate via the citramalate cycle. Inhibitory analysis showed that the 74-kDa protein is involved in tricarboxylic acid cycle, while the 148-kDa MDH takes part in the citramalate pathway. A single gene encoding synthesis of the isologous subunits of the MDH isoforms was found during molecular-biological investigations. The appearance in the studied bacterium of the tetrameric MDH isoform during growth in the presence of acetate is probably due to the increased level of mdh gene expression, revealed by the real-time PCR, the product of which in cooperation with the citramalate cycle enzymes plays an important role in acetate assimilation.
authors.affiliation: Voronezh State University, Voronezh, 394006, Russia. bc366@bio.vsu.ru
authors.forename: A T
authors.initials: AT
authors.lastname: Eprintsev
authors.forename: M A
authors.initials: MA
authors.lastname: Klimova
authors.forename: K D
authors.initials: KD
authors.lastname: Shikhalieva
authors.forename: D N
authors.initials: DN
authors.lastname: Fedorin
authors.forename: M T
authors.initials: MT
authors.lastname: Dzhaber
authors.forename: E I
authors.initials: EI
authors.lastname: Kompantseva
mesh_terms: D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D007527:Isoenzymes; D008291:Malate Dehydrogenase