Gentamicin-impregnated chitosan/nanohydroxyapatite/ethyl cellulose microspheres granules for chronic osteomyelitis therapy.
In this article gentamicin (GM) impregnated microspheres were used to extend the drug release time for the treatment of chronic osteomyelitis. The granules were prepared in solution and consisted of nanohydroxyapatite (nHA), chitosan (CS) and GM loaded ethyl cellulose (EC) microspheres. A rabbit model with chronic osteomyelitis was made by using staphylococcus aureus and morrhuate sodium and special inspection methods were used to test the curative effects of the granules, such as microbiological investigations, tissue, and X-ray observations. The granules were provided with excellent drug release properties, 49 days in vitro and 45 days in vivo, moreover, they showed almost no cytotoxic for fibroblast and osteoblast. The findings indicated that the GM-impregnated CS/nHA/EC microspheres granules showed outstanding curative effect. Generally, it can be concluded that the granules containing GM impregnated microspheres may be used effectively in the treatment of the chronic osteomyelitis.
Shi
Pujiang
P
The Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, China.
Zuo
Yi
Y
Li
Xiaowei
X
Zou
Qin
Q
Liu
Haohuai
H
Zhang
Li
L
Li
Yubao
Y
Morsi
Yos S
YS
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan / Q000494:pharmacology*; D002908:Chronic Disease; D017886:Durapatite / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D020007:Implants, Experimental; D008863:Microspheres; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010019:Osteomyelitis / Q000188:drug therapy* / Q000473:pathology; D011817:Rabbits; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013211:Staphylococcus aureus / Q000187:drug effects; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects / Q000382:microbiology / Q000473:pathology
D005839:Gentamicins; C013517:ethyl cellulose; D002482:Cellulose; D048271:Chitosan; D017886:Durapatite