[Effect of TFCC on proliferation of the first trimester human cytotrophoblast and its possible signal transduction pathway].
OBJECTIVE
To investigate the effect of total flavones from Cuscuta chinens is (TFCC) on proliferation of the first trimester human cytotrophoblast and its possible signal transduction pathway.


METHODS
The first trimester human trophoblast cells were isolated by trypsin/Dnasel digestion and discontinuous Percoll density gradient centrifugation. After identified by immunocytochemistry to Cytokeratin 7 and Vimentin, the trophoblast cells appared to more than 95% pure. MTT Assay was used to evaluate the effect of TFCC in vitro viability/proliferation of the first trimster human cytotrophoblasts, The PCNA expression of the first rimester human cytotrophoblasts were analyzed by the way of flow cytopmetry. The pERK level of the first trimester human cytotrophoblasts was analyzed by the way of western blotting.


RESULTS
TFCC could promote viabilitylproliferation and PCNA expression of the first trimester human cytotrophobia in vitro, which could activate the ERK 1/2 in time-dependent and dosage-dependent manner.
Ma
Hong-Xia
HX
The First Affiliated Hospital of Guangzhou Medicine College, Guangzhou 510120, China. doctormaxia_1974@yahoo.com.cn
You
Zhao-Ling
ZL
Liu
Hua
H
Song
Chun-Xia
CX
Mai
Zhi-Guang
ZG
D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D034182:Cuscuta / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D047309:Flavones / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
D047309:Flavones; D018809:Proliferating Cell Nuclear Antigen; D048049:Extracellular Signal-Regulated MAP Kinases