Characterization of two genes involved in chromate resistance in a Cr(VI)-hyper-resistant bacterium.
Mechanisms underlying chromate resistance in Cr(VI)-hyper-resistant Pseudomonas corrugata strain 28, isolated from a highly Cr(VI) polluted soil, were studied by analyzing its two Cr(VI)-sensitive mutants obtained by insertion mutagenesis. The mutants, namely Crg3 and Crg96, were characterized by the identification of disrupted genes, and by the high-throughput approach called Phenotype MicroArray (PM), which permitted the assay of 1,536 phenotypes simultaneously. Crg3 and Crg96 mutants were affected in a malic enzyme family gene and in a gene encoding for a RecG helicase, respectively. The application of PM provided a wealth of new information relating to the disrupted genes and permitted to establish that chromate resistance in P. corrugata strain 28 also depends on supply on NADPH required in repairing damage induced by chromate and on DNA integrity maintenance.
Decorosi
Francesca
F
Dipartimento di Biotecnologie Agrarie, Sez. Microbiologia, Università degli Studi di Firenze, Florence, Italy.
Tatti
Enrico
E
Mini
Annalisa
A
Giovannetti
Luciana
L
Viti
Carlo
C
D001426:Bacterial Proteins / Q000235:genetics*; D002840:Chromates / Q000494:pharmacology*; D002857:Chromium / Q000494:pharmacology*; D004265:DNA Helicases / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D008291:Malate Dehydrogenase / Q000235:genetics*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009249:NADP / Q000502:physiology; D010641:Phenotype; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology
D001426:Bacterial Proteins; D002840:Chromates; D004251:DNA Transposable Elements; D012989:Soil Pollutants; D002857:Chromium; C074702:chromium hexavalent ion; D009249:NADP; D008291:Malate Dehydrogenase; D004265:DNA Helicases