Reduced high-density lipoprotein 2b in non-obese type 2 diabetic patients analysed by a microfluidic chip method in a case-control study.
BACKGROUND
Alterations in high-density lipoprotein (HDL) subfractions, especially in the HDL2b subfraction, have been reported in type 2 diabetes mellitus (T2DM). However, new methods for convenient and reliable quantitation of HDL2b are yet to be developed.


METHODS
Thirty-eight patients with T2DM were enrolled and age-, sex- and body mass index (BMI)-matched controls were selected from the same population. A microfluidic chip method was employed to analyse serum HDL subfractions.


RESULTS
The microfluidic chip method revealed a significant reduction in HDL2b and its ratio to total HDL in T2DM patients. There was a reverse correlation for total HDL and HDL2b, and its ratio with triglycerides, homeostasis model assessment-insulin sensitivity index (HOMA-IS) and insulin resistance index (HOMA-IR).


CONCLUSIONS
We have shown a reduction of HDL2b and its ratio to total HDL by a novel chip method in T2DM patients. The significant correlation between HDL2b and HOMA-IS and HOMA-IR may have further predictive value in clinical utility.
Xian
Xunde
X
Institute of Cardiovascular Sciences, Peking University, Beijing, China.
Ma
Yahong
Y
Yang
Danhui D
DD
Huang
Wei
W
Wang
Yuhui
Y
Mueller
Odilo
O
Chang
Elain
E
Konigshofer
Yves
Y
Van-Cleve
Mark
M
Yang
Jinkui
J
Liu
George
G
D000368:Aged; D044466:Asian People; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006706:Homeostasis; D006801:Humans; D007333:Insulin Resistance; D008075:Lipoproteins, HDL / Q000097:blood; D053439:Lipoproteins, HDL2 / Q000097:blood*; D008297:Male; D046210:Microfluidic Analytical Techniques; D008875:Middle Aged
D001786:Blood Glucose; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2