title: Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA.
abstract: Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.
authors.affiliation: National Center for Antimicrobials and Infection Control, Statens Serum Institut, B.47, Artillerivej 5, DK-2300 Copenhagen.
authors.forename: Anders Rhod
authors.initials: AR
authors.lastname: Larsen
authors.forename: Richard
authors.initials: R
authors.lastname: Goering
authors.forename: Marc
authors.initials: M
authors.lastname: Stegger
authors.forename: Jodi A
authors.initials: JA
authors.lastname: Lindsay
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Gould
authors.forename: Jason
authors.initials: J
authors.lastname: Hinds
authors.forename: Marit
authors.initials: M
authors.lastname: Sørum
authors.forename: Henrik
authors.initials: H
authors.lastname: Westh
authors.forename: Kit
authors.initials: K
authors.lastname: Boye
authors.forename: Robert
authors.initials: R
authors.lastname: Skov
mesh_terms: D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial