title: Effects of surfactant replacement on alveolar overdistension and plasma cytokines in ventilator-induced lung injury.
abstract: BACKGROUND
  Overdistension of the lung causes ventilator-induced lung injury (VILI) accompanied by surfactant abnormalities and inflammatory changes. We investigated the effects of surfactant replacement on overdistension of the terminal airspaces and plasma cytokine levels in VILI.
  
  
  METHODS
  VILI was induced by high-pressure ventilation (HPV) in rats anesthetized with pentobarbital, followed by ventilation for 2 h in the maintenance mode (tidal volume=10 ml/kg, positive end-expiratory pressure = 7.5 cmH(2)O) with or without surfactant replacement. The sizes of the terminal airspaces were determined after fixing the lungs at an airway pressure of 10 cmH(2)O on deflation. Cytokine levels were assessed by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The mean ratio of the largest terminal airspace size class (> or = 64,000 microm(2)) was increased from 13.4% to 32.0% by HPV (P<0.05). After maintenance-mode ventilation, the ratio decreased to 16.1% with surfactant replacement (P<0.05), but increased to 44.6% without surfactant replacement (P<0.05). Mean macrophage inflammatory protein-2 (MIP-2) levels in the plasma increased from <0.02 to 6.9 ng/ml with HPV (P<0.05), and further increased to > or = 11.8 ng/ml, regardless of surfactant replacement after maintenance-mode ventilation. Similar tendencies were observed in the interleukin (IL)-6 and IL-10 levels. Tumor necrosis factor-alpha levels were almost negligible during the experiment.
  
  
  CONCLUSIONS
  In rats with VILI, surfactant replacement reversed overdistension of the terminal airspaces that may induce barotrauma, but not upregulation of MIP-2, IL-6, and IL-10 within 2 h.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Q
authors.initials: Q
authors.lastname: Wan
authors.forename: W
authors.initials: W
authors.lastname: Shi
authors.forename: H
authors.initials: H
authors.lastname: Qian
authors.forename: X
authors.initials: X
authors.lastname: Cui
authors.forename: W
authors.initials: W
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology* / Q000473:pathology; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D054426:Chemokine CXCL2 / Q000097:blood; D016207:Cytokines / Q000097:blood*; D006612:High-Frequency Ventilation / Q000009:adverse effects*; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D010100:Oxygen / Q000097:blood; D011175:Positive-Pressure Respiration; D011650:Pulmonary Alveoli / Q000293:injuries*; D011659:Pulmonary Gas Exchange / Q000187:drug effects; D011663:Pulmonary Surfactants / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013552:Swine; D012122:Ventilators, Mechanical / Q000009:adverse effects*
chemical_list: D054426:Chemokine CXCL2; C513296:Cxcl2 protein, rat; D016207:Cytokines; D015850:Interleukin-6; D011663:Pulmonary Surfactants; D016753:Interleukin-10; D002245:Carbon Dioxide; D010100:Oxygen