Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries.
Photodynamic antimicrobial therapy (PACT) promotes bacterial death as a result of the photosensitization of microbial components. This study evaluated the effect of PACT on dentine caries produced in situ. Over the course of 14 d, 20 volunteers wore intra-oral devices containing human dentine slabs that were treated 10 times daily with a 40% sucrose solution. Afterwards, the antimicrobial effect of toluidine blue O, associated with 47 or 94 J cm(-2) of a light-emitting diode, was evaluated. Before and after the treatments, dentine samples were analysed with regard to the total number of microorganisms, total streptococci, mutans streptococci, and lactobacilli. Significant reductions in the bacterial count were observed for PACT with both energy densities tested, with the following values observed for 47 and 94 J cm(-2) of irradiation: for total streptococci, 3.45 and 5.18; for mutans streptococci, 3.08 and 4.16; for lactobacilli, 3.24 and 4.66; and for total microorganisms, 4.29 and 5.43, respectively. The control, treated with 94 J cm(-2) of irradiation alone, was also effective against all bacteria. To conclude, PACT was effective in killing oral microorganisms present in dentine caries produced in situ and may be a useful technique for eliminating bacteria from dentine carious lesions before restoration.
Lima
Juliana P M
JP
Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza.
Sampaio de Melo
Mary A
MA
Borges
Fátima M C
FM
Teixeira
Alrieta H
AH
Steiner-Oliveira
Carolina
C
Nobre Dos Santos
Marinês
M
Rodrigues
Lidiany K A
LK
Zanin
Iriana C J
IC
D000328:Adult; D018441:Biofilms / Q000187:drug effects; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000188:drug therapy* / Q000382:microbiology; D003773:Dental Plaque / Q000382:microbiology; D003804:Dentin / Q000187:drug effects* / Q000382:microbiology; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects*; D008297:Male; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D016037:Single-Blind Method; D013291:Streptococcus / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects; D014048:Tolonium Chloride / Q000627:therapeutic use; D055815:Young Adult
D017319:Photosensitizing Agents; D014048:Tolonium Chloride