title: Multiplex molecular detection of respiratory pathogens in children with asthma exacerbation.
abstract: BACKGROUND
  Up to 80% of asthma exacerbations in white children are associated with viral upper respiratory infections. The relative importance of different respiratory pathogens and relevant microbiological data in Asian children are unclear. This study elucidated the epidemiology of respiratory infections in Hong Kong children with asthma exacerbation.
  
  
  METHODS
  A total of 209 children aged 3-18 years with asthma exacerbations and 77 controls with stable asthma were recruited. The severity of asthma exacerbations was assessed according to Global Initiative for Asthma guideline, and subjects aged 6 years or older performed exhaled nitric oxide and spirometric measurements. Nested multiplex polymerase chain reaction was used to detect 20 different respiratory pathogens.
  
  
  RESULTS
  Respiratory pathogens were detected in 105 (51.0%) subjects. The presence of any respiratory pathogen was associated with asthma exacerbation (odds ratio [OR], 2.77; 95% CI, 1.51-5.11; P < .001). Specifically, human rhinovirus (HRV) infection was more common among children with asthma exacerbation (OR, 2.38; 95% CI, 1.09-5.32; P = .018). All other pathogens or coinfections were not associated with asthmatic attacks. None of these respiratory infections was associated with the severity of asthma exacerbation (P > .15 for all). During peak HRV season in the winter of 2007 to 2008, this virus was detected in 46.4% of children with asthma exacerbations.
  
  
  CONCLUSIONS
  Respiratory viral infections are commonly found in children with asthma exacerbation, with HRV being the most important pathogen in our patients. Respiratory viral infection is a triggering factor for asthma exacerbation but does not correlate with its severity.
authors.affiliation: Department of Pediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong. tfleung@cuhk.edu.hk
authors.forename: Ting Fan
authors.initials: TF
authors.lastname: Leung
authors.forename: Man Yin
authors.initials: MY
authors.lastname: To
authors.forename: Apple C M
authors.initials: AC
authors.lastname: Yeung
authors.forename: Yun Sze
authors.initials: YS
authors.lastname: Wong
authors.forename: Gary W K
authors.initials: GW
authors.lastname: Wong
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Chan
mesh_terms: D000293:Adolescent; D001249:Asthma / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D006723:Hong Kong / Q000453:epidemiology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012008:Recurrence; D012137:Respiratory System / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000150:complications* / Q000453:epidemiology / Q000382:microbiology; D013147:Spirometry
chemical_list: D004269:DNA, Bacterial