Bi-phasic CD34+ cell mobilization of a syngeneic donor during prolonged G-CSF delivery.
BACKGROUND
G-CSF administration over 10 days and neutrophil cytapheresis have been reported in the literature, but the kinetics of CD34+ cells in this situation is unclear.


METHODS
A 42-year-old female underwent syngeneic transplantation for metastatic breast cancer. The recipient was in critical condition peri-transplant, therefore the donor received G-CSF for 13 days, during which eight cytaphereses for both PBPC and neutrophils were performed. Two peaks in CD34+ cells were noted; the first on Day 5 and the second on Days 10-13 of G-CSF administration; a total of 11.6 x 10(6)/kg CD34+ cells and 38.11 x 10(8)/kg neutrophils were infused. The recipient's ANC exceeded 0.1 x 10(9)/L on Day +3.


CONCLUSIONS
To our knowledge, this is the longest reported cytapheresis of CD34+ cells from a normal donor The bi-phasic pattern in the cytapheresis product is also of interest. It is an unusual pattern that suggests a profound and complicated alteration in the marrow progenitor cell pool. If substantiated, this finding may offer an alternative cytapheresis schedule for donors.
Arnold
S M
SM
Division of Hematology and Oncology, Markey Cancer Center, University of Kentucky, Chandler Medical Center, Kentucky 40536, USA.
Van Zant
G
G
Phillips
G
G
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism*; D001943:Breast Neoplasms / Q000556:secondary / Q000601:surgery*; D049109:Cell Proliferation; D016238:Cytapheresis; D004334:Drug Administration Schedule; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007700:Kinetics; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D011994:Recombinant Proteins; D014185:Transplantation, Isogeneic; D014427:Twins
D018952:Antigens, CD34; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor