[Comparson study of two types of constituents in seeds of Cassia obtusifolia before and after roasted].
OBJECTIVE
To establish methods of RP-HPLC respectively for content-determination of two types of constituents in the Cassia obtusifolia respectively, and tp determine the constituents between the raw and roasted seeds of C. obtusifolia in order to distinguish the difference of those seeds.


METHODS
HPLC systems consisted of Alltima C18 (4.6 mm x 150 mm, 5 microm), column temperature at 30 degrees C, flow rate of 1.0 mL x min(-1). Two different mobile phases and detection wavelengths: I , ACN-THF-0.1% H3 PO4 (17 : 3:80), 278 nm; II , MeOH-0.1% H3PO4 (79:21), 254 nm. Analyzed and compared the content of the two types of constituents between the raw and roasted seeds of C. obtusifolia.


RESULTS
The calibrate curves of 5 constituents were linear (r > 0.999 7). The precision and repeatability were perfect (RSD < 1.6%, 1.8%). The samples were stabile during 24 h.


CONCLUSIONS
The study provide a better universal reference for evaluating and controlling the quality of C. obtusifolia pieces.
Li
Guiliu
G
Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Xiao
Yongqing
Y
Zhang
Cun
C
Li
Li
L
Pang
Zhen
Z
D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D006358:Hot Temperature; D012639:Seeds / Q000737:chemistry*
D004365:Drugs, Chinese Herbal