title: A novel convenient method for high bacteriophage titer assay.
abstract: The recent various applications of phages (bacteriophages) including phage therapy have brought about a revival of phage investigation. The phage titer assay is indispensable for phage experiments. However, the conventional standard method is a plaque counting method which requires a little skill with tedious repeating operation. Furthermore, it is not directly applicable to high phage titers. In this paper, we describe a novel convenient "cross streak and paper disk assay method" for high titer concentration without plaque counting.
authors.affiliation: Department of Nutritional Sciences, Yasuda Women's University, Hiroshima 731-0153, Japan. kazuyosi@hiroshima-u.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nasu
authors.forename: Nozomi
authors.initials: N
authors.lastname: Shitami
authors.forename: Hitoko
authors.initials: H
authors.lastname: Toyoda
authors.forename: Hitomi
authors.initials: H
authors.lastname: Takemura
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Oomori
mesh_terms: D017123:Bacteriophage T7 / Q000254:growth & development; D001435:Bacteriophages / Q000254:growth & development*; D014776:Virus Cultivation / Q000379:methods*