[Expression and correlation of mast cell, Clusterin/apoJ and transforming growth factor-beta in the different stages of human dermal hemangioma].
OBJECTIVE
To investigate the expression and correlation of mast cell, Clusterin/apoJ and transforming growth factor-beta (TGF-beta) in the different stages of human dermal hemangioma.


METHODS
Immunohistochemical stain technique (SABC) and toluidine blue (TB) stain technique were respectively used to detect the expression level of Clusterin/apoJ and TGF-beta and the number of mast cells in the different stages of human dermal hemangioma.


RESULTS
There was remarkable statistical difference between the advanced stage of proliferative hemangioma and the other stages of proliferative hemangioma in the number of mast cell(P<0.01). There was also remarkable statistical difference between the early stage of involutional hemangioma and the other stages of involutional hemangioma in the number of mast cell (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the advanced stage of proliferative hemangioma was significantly higher than the other stages in proliferative hemangioma (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the early stage of involutional hemangioma was significantly higher than the other stages in involutional hemangioma (P<0.01). There was a significantly positive correlation between Clusterin/apoJ and TGF-beta in the different stages of human dermal hemangioma (P<0.01). The expression level of Clusterin/apoJ and TGF-beta was positively correlated with the number of mast cell in the different stages of human dermal hemangioma (P<0.01).


CONCLUSIONS
Mast cell may play a promotive role of apoptosis during the spontaneous regulate the expression of Clusterin/apoJ and promote the spontaneous involution of human dermal hemangioma.
Yuan
Wei-li
WL
Dept. of Stomatology, The Fourth Affiliated Hospital, China Medical University, Shenyang 110032, China.
Qin
Xing-jun
XJ
Wang
Xu-kai
XK
D017209:Apoptosis; D051152:Clusterin; D006023:Glycoproteins; D006391:Hemangioma; D006801:Humans; D008407:Mast Cells; D018832:Molecular Chaperones; D016212:Transforming Growth Factor beta
D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D016212:Transforming Growth Factor beta