title: Isolation of a novel leptin receptor gene promoter preferentially functioning in neuronal cells.
abstract: Leptin exerts its metabolic effects by binding to the leptin receptor (Ob-R). In humans, the promoter of the Ob-R gene-related protein (Ob-RGRP) gene and the B219/Ob-R promoter have been speculated to spatially and temporarily regulate Ob-R gene transcription; however, the promoter function of the Ob-R gene has not been directly analyzed. Using 5' rapid amplification of the cDNA end, we isolated novel ob-r transcripts starting from the 3' portions of the B219/ob-r 5'-untranslated region in the adult mouse brain. The proximal promoter containing these start sites showed robust activity in neuron-derived, but not non-neuronal cell lines. The promoter activity was comparable with that of the ob-rgrp promoter in a neuronal cell, but significantly weaker in non-neuronal cells. Deletion analyses identifying two enhancer elements were located in this promoter. The identification of a novel ob-r gene promoter might provide a useful tool to study neuron specific expression and hormonal regulation of the ob-r gene.
authors.affiliation: Department of Medicine and Molecular Science, Gunma University, Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Japan. tsato@showa.gunma-u.ac.jp
authors.forename: Tetsurou
authors.initials: T
authors.lastname: Satoh
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshino
authors.forename: Akiko
authors.initials: A
authors.lastname: Katano
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Takuya
authors.initials: T
authors.lastname: Tomaru
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shibusawa
authors.forename: Koshi
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yamada
authors.forename: Masatomo
authors.initials: M
authors.lastname: Mori
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D054411:Receptors, Leptin / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D054411:Receptors, Leptin; C515447:leptin receptor, mouse