Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA.
Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.
Larsen
Anders Rhod
AR
National Center for Antimicrobials and Infection Control, Statens Serum Institut, B.47, Artillerivej 5, DK-2300 Copenhagen.
Goering
Richard
R
Stegger
Marc
M
Lindsay
Jodi A
JA
Gould
Katherine A
KA
Hinds
Jason
J
Sørum
Marit
M
Westh
Henrik
H
Boye
Kit
K
Skov
Robert
R
D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*
D004269:DNA, Bacterial