New potential biocatalysts by laccase immobilization in PVA Cryogel type carrier.
Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process is recommended. Thus, the paper presents different ways of obtaining new biocatalysts by a laccase covalent immobilization on a macroporous carrier based on poly(vinyl alcohol) cryogel. Different procedures of covalent immobilization are described, the newly obtained biocatalysts being characterized. According to the experimental data, the stability of the immobilized enzyme increased and the pH profile changed, compared with those of the free enzyme.
Stanescu
Michaela Dina
MD
Aurel Vlaicu University, Elena Dragoi str. 2, 310330 Arad, Romania. stanescu@uav.ro
Fogorasi
Magdalena
M
Shaskolskiy
Boris L
BL
Gavrilas
Simona
S
Lozinsky
Vladimir I
VI
D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D059985:Cryogels; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism*; D010316:Particle Size; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties
D059985:Cryogels; D004800:Enzymes, Immobilized; D020100:Hydrogels; D011142:Polyvinyl Alcohol; D042845:Laccase