title: Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries.
abstract: Photodynamic antimicrobial therapy (PACT) promotes bacterial death as a result of the photosensitization of microbial components. This study evaluated the effect of PACT on dentine caries produced in situ. Over the course of 14 d, 20 volunteers wore intra-oral devices containing human dentine slabs that were treated 10 times daily with a 40% sucrose solution. Afterwards, the antimicrobial effect of toluidine blue O, associated with 47 or 94 J cm(-2) of a light-emitting diode, was evaluated. Before and after the treatments, dentine samples were analysed with regard to the total number of microorganisms, total streptococci, mutans streptococci, and lactobacilli. Significant reductions in the bacterial count were observed for PACT with both energy densities tested, with the following values observed for 47 and 94 J cm(-2) of irradiation: for total streptococci, 3.45 and 5.18; for mutans streptococci, 3.08 and 4.16; for lactobacilli, 3.24 and 4.66; and for total microorganisms, 4.29 and 5.43, respectively. The control, treated with 94 J cm(-2) of irradiation alone, was also effective against all bacteria. To conclude, PACT was effective in killing oral microorganisms present in dentine caries produced in situ and may be a useful technique for eliminating bacteria from dentine carious lesions before restoration.
authors.affiliation: Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza.
authors.forename: Juliana P M
authors.initials: JP
authors.lastname: Lima
authors.forename: Mary A
authors.initials: MA
authors.lastname: Sampaio de Melo
authors.forename: Fátima M C
authors.initials: FM
authors.lastname: Borges
authors.forename: Alrieta H
authors.initials: AH
authors.lastname: Teixeira
authors.forename: Carolina
authors.initials: C
authors.lastname: Steiner-Oliveira
authors.forename: Marinês
authors.initials: M
authors.lastname: Nobre Dos Santos
authors.forename: Lidiany K A
authors.initials: LK
authors.lastname: Rodrigues
authors.forename: Iriana C J
authors.initials: IC
authors.lastname: Zanin
mesh_terms: D000328:Adult; D018441:Biofilms / Q000187:drug effects; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000188:drug therapy* / Q000382:microbiology; D003773:Dental Plaque / Q000382:microbiology; D003804:Dentin / Q000187:drug effects* / Q000382:microbiology; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects*; D008297:Male; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D016037:Single-Blind Method; D013291:Streptococcus / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects; D014048:Tolonium Chloride / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D017319:Photosensitizing Agents; D014048:Tolonium Chloride