Genotoxicity assessment of chromium(III) propionate complex in the rat model using the comet assay.
The aim of the study was to assess genotoxicity of a chromium(III) propionate complex in rat's peripheral blood lymphocytes by the comet assay. The study was carried out on 18 12-weeks old female Wistar rats that were divided into three equal groups (six animals each): control (0), control-Cr(VI) and Cr(III)-tested rat fed ad libitum a basal diet and the diet supplemented either with 10 mg Cr(VI)/kg diet (given as K(2)Cr(2)O(7), equivalent of 1mg/kg body mass/day) or 1000 mg Cr(III)/kg diet (given as [Cr(3)O(O(2)CCH(2)CH(3))6(H(2)O)(3)]NO(3)), equivalent of 100mg Cr/kg body mass/day) for 4 weeks. High doses of supplementary Cr(III) were found to not affect body mass gain, feeding efficiency ratio and internal organ masses. Treatment of rats with the Cr(III) propionate complex, in contrast to Cr(VI), did not affect significantly the comet assay results in lymphocytes, which suggests that the compound does not exert genotoxic effects in rats.
Staniek
Halina
H
Department of Hygiene and Human Nutrition, Poznan University of Life Sciences, 31 Wojska Polskiego Street, 60-624 Poznan, Poland.
Kostrzewska-Poczekaj
Magdalena
M
Arndt
Magdalena
M
Szyfter
Krzysztof
K
Krejpcio
Zbigniew
Z
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002857:Chromium / Q000737:chemistry / Q000378:metabolism; D020552:Comet Assay; D004247:DNA / Q000187:drug effects / Q000235:genetics; D004435:Eating / Q000187:drug effects; D005260:Female; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects; D008856:Microscopy, Fluorescence; D009153:Mutagens; D009929:Organ Size / Q000187:drug effects; D011422:Propionates / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain / Q000187:drug effects
D009153:Mutagens; D011422:Propionates; D002857:Chromium; D004247:DNA; C029658:propionic acid