[Repair of radius bone defect with the nano-hydroxyapatite/collagen/calcium alginate].
OBJECTIVE
To explore the osteogenic potential of the nano-hydroxyapatite/collagen/calcium alginate composite implanted in animals.


METHODS
Eighteen 3-month-old New Zealand white rabbits were adopted to prepare 15 mm segmental defect model at the middle part of radius. Rabbit models were randomly divided into experimental group and blank control group. Nano-hydroxyapatite/collagen/calcium alginate was implanted into the defects of experimental group. Four, 8, and 12 weeks after operation, all specimens were examined by X-ray and histological methods.


RESULTS
All the 18 rabbit models entered the final analysis. X-ray showed that osteotylus was seen in the whole defect area in the experimental group 12 weeks after operation, during which osteogenesis was more obvious than in weeks 4 and 8 and the bridge grafting of defect area was obviously visible. In the blank control group, osteotylus was only observed at the two ends of the defects, and no osteogenesis was found in the central part of the defect area. Histological examination showed that new osteoid formation was seen in internal porous zone in the experimental group in weeks 4 and 8; in week 12, more woven bone-like tissues were visible and trabecular-like structure was formed.


CONCLUSIONS
The nano-hydroxyapatite/ collagen/calcium alginate has good osteogenic potential.
Lan
Xiao-Yong
XY
Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
Zhou
Chu-Song
CS
Tian
Jing
J
Lü
Zhi-De
ZD
Xu
Qi-Fei
QF
Feng
Qing-Ling
QL
Huang
Zhi
Z
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D010012:Osteogenesis; D011817:Rabbits; D011884:Radius / Q000033:anatomy & histology*; D023822:Tissue Engineering; D014945:Wound Healing
D000464:Alginates; D006603:Hexuronic Acids; C509317:nano-hydroxyapatite-collagen; D020723:Glucuronic Acid; D003094:Collagen; D017886:Durapatite