title: Applications of isothermal titration calorimetry in biophysical studies of G-quadruplexes.
abstract: G-quadruplexes are higher-order nucleic acids structures formed by G-rich sequences that are stabilized by tetrads of hydrogen-bonded guanine bases. Recently, there has been growing interest in the study of G-quadruplexes because of their possible involvement in many biological processes. Isothermal titration calorimetry (ITC) has been proven to be a useful tool to study the energetic aspects of G-quadruplex interactions. Particularly, ITC has been applied many times to determine the thermodynamic properties of drug-quadruplex interactions to screening among various drugs and to address drug design. In the present review, we will focus on the ITC studies of G-quadruplex structures and their interaction with proteins and drugs and the most significant results will be discussed.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via Ponte Don Melillo, 84084, Fisciano (SA), Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Pagano
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via Ponte Don Melillo, 84084, Fisciano (SA), Italy.
authors.forename: Carlo Andrea
authors.initials: CA
authors.lastname: Mattia
authors.affiliation: Dipartimento di Chimica, Università di Napoli Federico II, via Cintia, 80126, Napoli, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Giancola
mesh_terms: D002151:Calorimetry / Q000379:methods*; D054856:G-Quadruplexes; D013816:Thermodynamics