[Effects of cyclic mechanical stretch on the activity of STAT3 in human bronchial epithelial cell (16HBEC)].
OBJECTIVE
To study the effects of cyclic mechanical stretch on the activity and expression of STAT3 in Human bronchial epithelial Cell (16HBEC).


METHODS
The 16HBECs were subjected to cyclic mechanical stretch by the four-point bending system at 0.5 Hz of 1 kPa, 2 kPa, 3 kPa, 4 kPa for 30 min or for 30 min, 1 h, 3 h, and 6 h of 3 kPa respectively. The mRNA level of STAT3 was semi-quantified by RT-PCR; STAT3 protein and phosphorylated STAT3 were detected by western blot.


RESULTS
Phosphorylation level of STAT3 Tyr705 was higher under 3 kPa cyclic mechanical stretch than the others. 3 kPa stretch phosphorylatd STAT3 Tyr705 in 16HBE cells in a time-dependent manner. The phosphorylation of STAT3-Tyr705 was significantly increased at 30 min compared with the control group (P<0.05), and reached the highest phosphorylation level at 1 h, then showed a sharp descending tendency. There was no effect of 3 kPa stretch on the phosphorylation of STAT3 Ser727, expression of STAT3 protein or mRNA levels.


CONCLUSIONS
STAT3 played an important role in the response and signal transduction of 16HBECs to the cyclic mechanical stretch stimulation through the phosphorylation of STAT3 Tyr705.
Ning
Yun-Ye
YY
Laboratory of Pulmonary Disease, Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Han
Su-Xia
SX
Wang
Tao
T
Xu
Dan
D
Wen
Fu-Qiang
FQ
D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D013314:Stress, Mechanical; D013997:Time Factors
D012333:RNA, Messenger; D050796:STAT3 Transcription Factor