title: Purification, characterization, and crystallization of the adhesive domain of SdrD from Staphylococcus aureus.
abstract: The adhesive domain of SdrD from Staphylococcus aureus was solubly expressed in Escherichia coli in high yield. After a series of purification steps, the purified protein was >95% pure, which was SdrD from S. aureus identified by SDS-PAGE and MALDI-TOF MS. Crystals were grown at 18 degrees C using 25% polyethylene glycol 3350 as precipitant. Diffraction by the crystal extends to 1.65A resolution, and the crystal belongs to the space group C2, with the unit cell parameters a=133.3, b=58.3, c=112.3A, alpha=90.00, beta=111.14, gamma=90.00.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, People's Republic of China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Xiang
authors.forename: Jinlan
authors.initials: J
authors.lastname: Gao
authors.forename: Jia
authors.initials: J
authors.lastname: Hu
authors.forename: Shiying
authors.initials: S
authors.lastname: Miao
authors.forename: Linfang
authors.initials: L
authors.lastname: Wang
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.forename: Shentao
authors.initials: S
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D003460:Crystallization; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; C115915:SdrD protein, Staphylococcus aureus