title: Leishmania(Leishmania) chagasi in captive wild felids in Brazil.
abstract: This study used a PCR-RFLP test to determine the presence of Leishmania (Leishmania) chagasi in 16 captive wild felids [seven Puma concolor (Linnaeus, 1771); five Panthera onca (Linnaeus, 1758) and four Leopardus pardalis (Linnaeus, 1758)] at the zoological park of the Federal University of Mato Grosso, Brazil. Amplification of Leishmania spp. DNA was seen in samples from five pumas and one jaguar, and the species was characterized as L. chagasi using restriction enzymes. It is already known that domestic felids can act as a reservoir of L. chagasi in endemic areas, and further studies are necessary to investigate their participation in the epidemiological chain of leishmaniasis.
authors.affiliation: Federal University of Mato Grosso, 78060-900 Cuiabá, MT, Brasil.
authors.forename: Magyda A A
authors.initials: MA
authors.lastname: Dahroug
authors.forename: Arleana B P F
authors.initials: AB
authors.lastname: Almeida
authors.forename: Valéria R F
authors.initials: VR
authors.lastname: Sousa
authors.forename: Valéria
authors.initials: V
authors.lastname: Dutra
authors.forename: Nívea C M R
authors.initials: NC
authors.lastname: Turbino
authors.forename: Luciano
authors.initials: L
authors.lastname: Nakazato
authors.forename: Roberto L
authors.initials: RL
authors.lastname: de Souza
mesh_terms: D000818:Animals; D000836:Animals, Zoo / Q000469:parasitology*; D001938:Brazil; D016054:DNA, Protozoan / Q000032:analysis*; D045989:Felidae / Q000469:parasitology*; D005784:Gene Amplification; D018314:Leishmania infantum / Q000235:genetics / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000469:parasitology / Q000662:veterinary*; D046009:Panthera / Q000469:parasitology; D016133:Polymerase Chain Reaction; D046011:Puma / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan