title: Purinergic receptor P2Y, G-protein coupled, 2 (P2RY2) gene is associated with cerebral infarction in Japanese subjects.
abstract: G-protein-coupled purinergic receptor P2Y2 (P2RY2) has an important role in the process of atherosclerosis related to cerebral infarction (CI). The aim of this study was to investigate the relationship between the P2RY2 gene and CI through a haplotype-based case-control study, including the separate analysis of two gender groups. A total of 237 CI patients and two control groups (control 1, 254; control 2, 255) were genotyped for five single nucleotide polymorphisms (SNPs) in the human P2RY2 gene (rs4944831, rs1783596, rs4944832, rs4382936, rs10898909). Among women, the distribution of the dominant rs4944832 phenotype (GG vs. GA+AA) differed significantly between the CI patients and the control 1 group (P=0.043) and between the CI patients and the control 2 group (P=0.029). Logistic regression analysis showed that the GG genotype of rs4944832 was significantly more prevalent in the female CI patients than in the control 1 (P=0.021) and control 2 groups (P=0.005). For all subjects, the overall distribution of the haplotype established by rs1783596-rs4382936-rs10898909 was significantly different between the CI patients and the control 1 group (P=0.027). For all subjects, the frequency of the T-A-G haplotype (rs1783596-rs4382936-rs10898909) was also significantly higher (P=0.031), whereas the frequency of the T-C-G haplotype (rs1783596-rs4382936-rs10898909) was significantly lower (P=0.029) in the CI patients than in the control 1 group. The present results indicate that the T-A-G haplotype of the human P2RY2 gene is a susceptibility haplotype for CI in Japanese subjects, and that the GG genotype is a genetic marker for CI, particularly in Japanese women.
authors.affiliation: Division of Laboratory Medicine, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Wang
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nakayama
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sato
authors.forename: Mai
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Izumi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kasamaki
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Ohta
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Soma
authors.forename: Noriko
authors.initials: N
authors.lastname: Aoi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ozawa
authors.forename: Yitong
authors.initials: Y
authors.lastname: Ma
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Doba
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Hinohara
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000453:epidemiology* / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D018048:Receptors, Purinergic P2 / Q000235:genetics*; D058473:Receptors, Purinergic P2Y2; D012044:Regression Analysis; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012737:Sex Factors
chemical_list: C546385:P2RY2 protein, human; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2; D004247:DNA