Interleukin-6 leads to interleukin-10 production in several human multiple myeloma cell lines. Does interleukin-10 enhance the proliferation of these cells?
Multiple myeloma (MM) is characterised as a malignant plasma cell proliferation. Interleukin-6 (IL-6) is an important cytokine in the proliferation of the multiple myeloma cells. Interleukin-10 (IL-10) is involved in the terminal differentiation of B cells into plasma cells and enhances the proliferation of B cells. Elevated IL-10 levels were detected in patients with MM, relating to the clinical manifestation of the disease. In this study it was investigated the effect of exogenous IL-6 on the IL-10 production and the proliferative effect of IL-6 and IL-10 in human multiple myeloma cell lines. The ten cell lines had different sources: blood, ascitic fluid, bone marrow, peritoneum and lymph node.


RESULTS
(1) Four cell lines produced IL-10 (up to 179 pg/ml) spontaneously. IL-6 increased the IL-10 production up to 1626 pg/ml in 6/10 cell lines. With IL-6 receptor antagonist (IL-6RA) the values of IL-10 were in the range of untreated samples (spontaneous cytokine production). IL-6RA counteracted the increased IL-10 production induced by IL-6 treatment and decreased the high values of IL-10 significantly. (2) IL-6 enhanced the proliferation (up to 160%) in 9/10 cell lines, IL-10 enhanced the proliferation (up to 170%) in 7/10 cell lines. There was a significant correlation between the proliferative effects of IL-6 and IL-10.


CONCLUSIONS
It is reported for the first time that IL-6 leads to a marked production of IL-10 and that this cytokine is an IL-6 related growth factor for MM cells. The findings of this study can be important in the therapeutic modalities of multiple myeloma.
Kovacs
Eva
E
evakovacsbenke@hotmail.com
D001201:Ascites / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D008198:Lymph Nodes / Q000473:pathology; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D019899:Paracrine Communication; D010994:Pleura / Q000473:pathology; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology
C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D009363:Neoplasm Proteins; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins; D016753:Interleukin-10