Mouse orthotopic lung cancer model induced by PC14PE6.
OBJECTIVE
This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells.


METHODS
Three cell doses (0.5x10(6); 1x10(6); 2x10(6)) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells.


RESULTS
The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average life span of the three groups after inoculation was 14 days in the 2x10(6) cells inoculum group; 25 days in the 1x10(6) cells inoculum group; and 32 days in the 0.5x10(6) cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax.


CONCLUSIONS
This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis.
Cui
Zheng Yun
ZY
Medical Nano Element Development Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Ahn
Jin Seok
JS
Lee
Jee Yun
JY
Kim
Won Seog
WS
Lim
Ho Yeong
HY
Jeon
Hyun Jung
HJ
Suh
Soo Won
SW
Kim
Jin Hoon
JH
Kong
Won Ho
WH
Kang
Ji Min
JM
Nam
Do Hyun
DH
Park
Keunchil
K