Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea.
To investigate the population structure of five dog breeds in South Korea and to validate polymorphic microsatellite markers for the parentage test, microsatellite loci analyses were conducted for two Korean native dog breeds, Poongsan and Jindo, and three imported dog breeds, German Shepherd, Beagle and Greyhound. Overall genetic diversity was high across all dog breeds (expected heterozygosity range: 0.71 to 0.85), although breeds differed in deviations from Hardy-Weinberg equilibrium (HWE). Significant reduction of heterozygosity in the Poongsan and Greyhound breeds was caused by non-random mating and population substructure within these breeds (the Wahlund effects). The close relationship and high degree of genetic diversity for two Korean native dog breeds were substantial. The mean polymorphism information content value was highest in Jindos (0.82) and Poongsans (0.81), followed by Beagles (0.74), Greyhounds (0.72), and German Shepherds (0.66). Accumulated exclusion power values, as an indication of marker validity for parentage tests, were varied but very high across breeds, 0.9999 for Jindos, Poongsans, and Beagles, 0.9997 for Greyhounds, and 0.9995 for German Shepherds. Taken together, the microsatellite loci investigated in this study can serve as suitable markers for the parentage test and as individual identification to establish a reliable pedigree verification system of dog breeds in South Korea. This study also stresses that the population subdivision within breeds can become an important cause of deviation from HWE in dog breeds.
Kang
Byeong-Teck
BT
BK21 Program of Basic and Diagnostic Veterinary Specialist Program for Animal Diseases and Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, South Korea.
Kim
Kyung-Seok
KS
Min
Mi-Sook
MS
Chae
Young-Jin
YJ
Kang
Jung-Won
JW
Yoon
Junghee
J
Choi
Jihye
J
Seong
Je-Kyung
JK
Park
Han-Chan
HC
An
Junghwa
J
Lee
Mun-Han
MH
Park
Hee-Myung
HM
Lee
Hang
H
D000818:Animals; D001947:Breeding / Q000379:methods; D004285:Dogs / Q000235:genetics*; D005821:Genetic Techniques / Q000662:veterinary; D014644:Genetic Variation; D007723:Korea; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D010375:Pedigree; D010802:Phylogeny