[Analysis of biochemical indices and efficient ingredients in autotetraploid of Dioscorea zingibiernsis].
OBJECTIVE
To improve the yield and quality of Dioscrea zingibiernsis.


METHODS
The Biochemical indices and the diosgenin's content were analysed in the autotetraploid lines.


RESULTS
The results showed that the activities of APX, SOD and POD in most of autotetraploid lines were higher than that in diploid line or close to it, there was also difference between autotetraploid lines and control lines in the SDS-PAGE of soluble proteins. The content of diosgenin in most autotetraploid plantlets were higher than that in the control.


CONCLUSIONS
There were difference between autotetraploid and control lines in the content of diosgenin and Biochemical indices, therefore, inducing autotetraploid could be an effective way to breeding the superior varieties.
Xie
Cai-Xia
CX
Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. nanyangxcx@163.com
Dong
Cheng-Ming
CM
Gao
Shan-Lin
SL
Qin
Hui-Zhen
HZ
Bai
Yan
Y
D027742:Dioscorea / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004144:Diosgenin / Q000032:analysis* / Q000302:isolation & purification; D004171:Diploidy; D004591:Electrophoresis, Polyacrylamide Gel; D010544:Peroxidases / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis; D011123:Polyploidy; D013482:Superoxide Dismutase / Q000378:metabolism; D046509:Tissue Culture Techniques
D010940:Plant Proteins; D010544:Peroxidases; D013482:Superoxide Dismutase; D004144:Diosgenin