[The effect of Nifedipine and mechanical strain on matrix metalloproteinase expression in human periodontal ligament fibroblast].
OBJECTIVE
To investigate the effect of Nifedipine on matrix metalloproteinase (MMP)-10 and MMP-13 expression in human periodontal ligament fibroblast (HPDLF) induced by mechanical strain in vitro.


METHODS
HPDLF was divided into four groups at random by mechanical strain of elongation 0%, 8%, 12% and 16%. Each group was divided into four subgroups again by the concentration of Nifedipine 0, 10, 30, 50 microm. The cells were imposed to state mechanical strain 12 h after incubated with Nifedipine 1 h. And then immunohistochemical staining was used to investigate the expressions of MMP-10 and MMP-13 in intracytoplasm.


RESULTS
In 0% group, there was no significant difference of MMP-10 and MMP-13 expression in all subgroups (P>0.05). The expressions of MMP-10 and MMP-13 were high in group 8%, 12% and 16% without Nifedipine, and increasing significantly with elongation (P<0.001). The expressions of MMP-10 and MMP-13 decreased with dose increasing (P<0.001) in HPDLF after imposed to Nifidepine.


CONCLUSIONS
The expressions of MMP-10 and MMP-13 induced by mechanical strain were inhibited by Nifedipine, which suggested calcium ions channels participate in expressions of MMP-10 and MMP-13 induced by loading.
Ni
Hui
H
Dept. of Orthodontics, School of Stomatology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Zhang
Miao-miao
MM
Dai
Jia-yin
JY
Sun
Miao
M
D005347:Fibroblasts; D006801:Humans; D020782:Matrix Metalloproteinases; D009543:Nifedipine; D010513:Periodontal Ligament; D012333:RNA, Messenger
D012333:RNA, Messenger; D020782:Matrix Metalloproteinases; D009543:Nifedipine