title: Mapping 3-dimensional neovessel organization steps using micro-computed tomography in a murine model of hindlimb ischemia-brief report.
abstract: OBJECTIVE
  Studying the mechanisms of neovascularization and evaluating the effects of proangiogenic strategies require accurate analysis of the neovascular network. We sought to evaluate the contribution of the microcomputed tomography (mCT) providing high-resolution 3-dimensional (3D) structural data, to a better comprehension of the well-studied mouse hindlimb postischemic neovascularization.
  
  
  RESULTS
  We showed a predominant arteriogenesis process in the thigh and a predominant angiogenesis-related process in the tibiofibular region, in response to ischemia during the first 15 days. After 15 days, mCT quantitative analysis reveals a remodeling of arterial neovessels and a regression depending on the restoration of the blood flow. We provided also new mCT data on the rapid and potent angiogenic effects of mesenchymal stem cell therapy on vessel formation and organization. We discussed the contribution of this technique compared with or in addition to data generated by the more conventional approaches.
  
  
  CONCLUSIONS
  This study demonstrated that optimized mCT is a robust method for providing new insights into the 3D understanding of postischemic vessel formation.
authors.affiliation: Inserm U828, Pessac, Université Victor Ségalen, Bordeaux, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Oses
authors.forename: Marie-Ange
authors.initials: MA
authors.lastname: Renault
authors.forename: Rémi
authors.initials: R
authors.lastname: Chauvel
authors.forename: Lionel
authors.initials: L
authors.lastname: Leroux
authors.forename: Cécile
authors.initials: C
authors.lastname: Allières
authors.forename: Benjamin
authors.initials: B
authors.lastname: Séguy
authors.forename: Jean-Marie Daniel
authors.initials: JM
authors.lastname: Lamazière
authors.forename: Pascale
authors.initials: P
authors.lastname: Dufourcq
authors.forename: Thierry
authors.initials: T
authors.lastname: Couffinhal
authors.forename: Cécile
authors.initials: C
authors.lastname: Duplàa
mesh_terms: D000818:Animals; D001464:Barium; D003287:Contrast Media; D004195:Disease Models, Animal; D006614:Hindlimb / Q000098:blood supply*; D021621:Imaging, Three-Dimensional; D007511:Ischemia / Q000473:pathology* / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D009387:Neoprene; D018919:Neovascularization, Physiologic; D016491:Peripheral Vascular Diseases / Q000473:pathology / Q000601:surgery; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D003287:Contrast Media; D001464:Barium; D009387:Neoprene