title: Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity.
abstract: In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Nekrasov
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Martine
authors.initials: M
authors.lastname: Batoux
authors.forename: Milena
authors.initials: M
authors.lastname: Roux
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Chu
authors.forename: Severine
authors.initials: S
authors.lastname: Lacombe
authors.forename: Alejandra
authors.initials: A
authors.lastname: Rougon
authors.forename: Pascal
authors.initials: P
authors.lastname: Bittel
authors.forename: Marta
authors.initials: M
authors.lastname: Kiss-Papp
authors.forename: Delphine
authors.initials: D
authors.lastname: Chinchilla
authors.forename: H Peter
authors.initials: HP
authors.lastname: van Esse
authors.forename: Lucia
authors.initials: L
authors.lastname: Jorda
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schwessinger
authors.forename: Valerie
authors.initials: V
authors.lastname: Nicaise
authors.forename: Bart P H J
authors.initials: BP
authors.lastname: Thomma
authors.forename: Antonio
authors.initials: A
authors.lastname: Molina
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
authors.forename: Cyril
authors.initials: C
authors.lastname: Zipfel
mesh_terms: D017360:Arabidopsis / Q000276:immunology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism; D007113:Immunity, Innate; D010935:Plant Diseases / Q000276:immunology*; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C545106:EFR protein, Arabidopsis; D050956:HSP40 Heat-Shock Proteins; D051192:Receptors, Pattern Recognition; C545107:SDF2 protein, Arabidopsis; C077221:BIP protein, Arabidopsis