A fluorogenic, nucleic acid directed "click" reaction.
A fluorogenic, nucleic acid directed, Cu(+)-catalyzed chemical reaction was developed that allows sequence-specific detection of nucleic acids at concentrations as low as 20 nM through monitoring of the increase of the fluorescence intensity. A single nucleotide mismatch in the template strand leads to the complete inhibition of this reaction. We observed that a Cu(+) ion stabilized with a water-soluble ligand is a significantly more efficient catalyst than free Cu(+).
Jentzsch
Elmar
E
Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
Mokhir
Andriy
A
D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D057230:Limit of Detection; D009696:Nucleic Acids / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D003300:Copper