Succinatimonas hippei gen. nov., sp. nov., isolated from human faeces.
A novel strictly anaerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped, Gram-negative bacterium (YIT 12066T) was isolated from human faeces. The isolate was negative for catalase, oxidase, urease, hydrolysis of aesculin and gelatin, nitrate reduction and indole production. The major end products of glucose metabolism were succinate and acetate. The major cellular fatty acids (>10%) were C14:0, C18:1omega7c, C18:1omega9c, C16:1omega7c and C16:0. The G+C content of the DNA was 40.3 mol%. 16S rRNA gene sequence analysis showed that strain YIT 12066T was most closely related to members of the family Succinivibrionaceae, with sequence similarity of 92-87%. However, some phenotypic characteristics such as cellular morphology and the major fatty acid profile of strain YIT 12066T were markedly different from those of other members of the family Succinivibrionaceae. On the basis of both phylogenetic and phenotypic evidence, it is suggested that strain YIT 12066T represents a novel species in a new genus, for which the name Succinatimonas hippei gen. nov., sp. nov. is proposed; the type strain of Succinatimonas hippei is YIT 12066T (=DSM 22608T =JCM 16073T).
Morotomi
Masami
M
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 186-8650 Tokyo, Japan.
Nagai
Fumiko
F
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 186-8650 Tokyo, Japan.
Watanabe
Yohei
Y
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 186-8650 Tokyo, Japan.
Tanaka
Ryuichiro
R
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 186-8650 Tokyo, Japan.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S