[Effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells].
OBJECTIVE
To investigate the effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells.


METHODS
The proliferation inhibitory rate was evaluated by MTT assay. The telomerase activity was analyzed by TRAP-ELISA, and the expression of hTERT mRNA was determined by semi-quantitative RT-PCR.


RESULTS
Matrine displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against SW1116 cells. Compared with control group, the telomerase activity and the expression of hTERT mRNA decreased significantly (P < 0.05 or P < 0.01) in matrine group.


CONCLUSIONS
Matrine can inhibit the telomerase activity by depressing the expression of hTERT in SW1116 cells and inhibiting cell proliferation.
Zhou
Xi-Han
XH
Department of Gastroenterology, Youjiang Medical College for Nationalities, Baise 533000, China. ZHXH@ymcn.gx.cn
Wei
Xing
X
Huang
Zan-Song
ZS
Cen
Chao
C
Yin
Yi-Xia
YX
Qin
Yue-Qiu
YQ
Su
Qian-Bo
QB
D000470:Alkaloids / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D011807:Quinolizines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029909:Sophora / Q000737:chemistry*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D000093842:Matrines
D000470:Alkaloids; D000970:Antineoplastic Agents; D011807:Quinolizines; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase; D000093842:Matrines