Poly(ethylene glycol)-block-poly(glycidyl methacrylate) with oligoamine side chains as efficient gene vectors.
Well-defined diblock copolymers, poly(ethylene glycol)-block-poly(glycidyl methacrylate)s (PEG-b-PGMAs), with different poly(glycidyl methacrylate) (PGMA) chains, were prepared via atom transfer radical polymerization (ATRP) from the same macromolecular initiator 2-bromoisobutyryl-terminated poly(ethylene glycol) (PEG). Ethyldiamine (EDA), diethylenetriamine (DETA), triethylenetetramine (TETA), and polyethyleneimine (PEI) with an M(W) of 400 (PEI(400)) were used to decorate PEG-b-PGMAs to get the cationic polymers PEG-b-PGMA- oligoamines. These cationic polymers possessed high buffer capability and could condense plasmid DNA (pDNA) into nanoscaled complexes of 125-530 nm. These complexes showed the positive zeta potential of 20-35 mV at N/P ratios of 10-50. Most of them exhibited very low cytotoxicity and good transfection efficiency in 293T cells. The presence of the serum medium did not decrease the transfection efficiency due to the steric stabilization of the PEG chains.
Ma
Ming
M
Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, P. R. China.
Li
Feng
F
Chen
Fu-jie
FJ
Cheng
Si-xue
SX
Zhuo
Ren-xi
RX
D000588:Amines / Q000737:chemistry*; D002021:Buffers; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004587:Electrophoresis, Agar Gel; D004852:Epoxy Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D008856:Microscopy, Fluorescence; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D012542:Scattering, Radiation; D044967:Serum; D017186:Titrimetry; D014162:Transfection
D000588:Amines; D002021:Buffers; D004852:Epoxy Compounds; D008689:Methacrylates; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; C007870:glycidyl methacrylate