title: An anti-inflammatory lectin from Luetzelburgia auriculata seeds inhibits adhesion and rolling of leukocytes and modulates histamine and PGE2 action in acute inflammation models.
abstract: OBJECTIVE
  To study and characterize the in vivo effect of the lectin from Luetzelburgia auriculata seed on acute inflammation models.
  
  
  METHODS
  The lectin was purified from the crude saline extract by affinity chromatography on a guar-gum matrix. Native, heat-treated, and digested lectin was evaluated for anti-inflammatory activity by using peritonitis and paw edema models. The anti-inflammatory activity was characterized by intravital microscopy, nitric oxide production, and myeloperoxidase activity.
  
  
  RESULTS
  The lectin exhibited anti-inflammatory activity (2 mg/kg) on both models, reducing local myeloperoxidase activity. Galactose or heat treatment (100 degrees C, 10 min) reduced anti-inflammatory action. Anti-inflammation involves the inhibition of adhesion and rolling of leukocytes along with augmentation of nitric oxide in serum. The lectin inhibited the edematogenic effect of histamine and prostaglandins (PGE2) but did not alter the chemoattractant effect of IL-8.
  
  
  CONCLUSIONS
  The results indicate that this lectin is a potent anti-inflammatory molecule. Its effects engage diverse modulatory events.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, CE, Brazil. nylane@ufc.br
authors.forename: N M N
authors.initials: NM
authors.lastname: Alencar
authors.forename: R S B
authors.initials: RS
authors.lastname: Oliveira
authors.forename: J G
authors.initials: JG
authors.lastname: Figueiredo
authors.forename: I J M
authors.initials: IJ
authors.lastname: Cavalcante
authors.forename: M P V
authors.initials: MP
authors.lastname: Matos
authors.forename: F Q
authors.initials: FQ
authors.lastname: Cunha
authors.forename: J V S
authors.initials: JV
authors.lastname: Nunes
authors.forename: L R
authors.initials: LR
authors.lastname: Bomfim
authors.forename: M V
authors.initials: MV
authors.lastname: Ramos
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D002351:Carrageenan; D002448:Cell Adhesion / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D015232:Dinoprostone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000737:chemistry*; D005690:Galactose / Q000378:metabolism; D006633:Histamine Antagonists; D007202:Indicators and Reagents; D007249:Inflammation / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D036904:Leukocyte Rolling / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D010538:Peritonitis / Q000139:chemically induced / Q000188:drug therapy; D009195:Peroxidase / Q000378:metabolism; D037121:Plant Lectins / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006633:Histamine Antagonists; D007202:Indicators and Reagents; D037121:Plant Lectins; D009569:Nitric Oxide; D002351:Carrageenan; D009195:Peroxidase; D015232:Dinoprostone; D005690:Galactose