Impaired mitochondrial energy metabolism and kinetic properties of cytochrome oxidase following acute aluminium phosphide exposure in rat liver.
The present study was designed with an aim to analyze the effect of acute aluminium phosphide (ALP) exposure (10mg/kg b.wt, intragastrically) on the kinetic characteristics of cytochrome oxidase and energy metabolism in male Wistar rat liver mitochondria. Liver mitochondrial preparations from ALP-treated rats exhibited significant decrease (66%) in the activity of cytochrome oxidase suggesting that there was a decrease in the catalytic efficiency of the active oxidase molecules on ALP treatment. The decreased activity of cytochrome oxidase with altered NADH and succinic dehydrogenase activities might have contributed towards a significant decline in state 3 and state 4 respiration as observed. These alterations in the electron transport chain complexes in turn adversely affected the ATP synthesis rate as well as ATP levels in the mitochondria isolated from treated rats. The alterations in the respiratory chain, was followed by enhanced lipid peroxidation in rat liver mitochondria which might have further contributed to change in the fluidity of membrane as depicted by decreased fatty acid content of liver mitochondria. However, no significant change was observed in cholesterol and phospholipids content in our study. The present study thus highlights the significance of altered mitochondrial respiratory chain functions and membrane integrity after acute ALP exposure.
Dua
Raina
R
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.
Sunkaria
Aditya
A
Kumar
Vijay
V
Gill
Kiran Dip
KD
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000378:metabolism; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005227:Fatty Acids / Q000737:chemistry; D066298:In Vitro Techniques; D007700:Kinetics; D007928:Lethal Dose 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism; D010575:Pesticides / Q000633:toxicity*; D010720:Phosphines / Q000633:toxicity*; D010743:Phospholipids / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013696:Temperature
D017607:Aluminum Compounds; D005227:Fatty Acids; D010575:Pesticides; D010720:Phosphines; D010743:Phospholipids; D000255:Adenosine Triphosphate; D002784:Cholesterol; C001864:aluminum phosphide; D013385:Succinate Dehydrogenase; D009252:NADPH Dehydrogenase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases