The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction.
Topographical cues play an important role in influencing cellular behavior and are considered as significant parameters to be controlled in tissue engineering applications. This work investigated the biocompatibility with regard to scaffold architecture and topographical effect of nanofibrous scaffolds on the in vivo and in vitro foreign body reaction. Random and aligned polycaprolactone (PCL) nanofibers were fabricated by electrospinning technique, with diameters of 313 +/- 5 nm and 506 +/- 24 nm, respectively. Primary monocytes isolated from five human donors were cultured on PCL nanofibers, PCL film, and RGD-coated glass in vitro and cell density and morphology was evaluated at time points of day 0 (2 h), day 3, day 7, and day 10. The in vivo study was carried out by implanting PCL nanofibers and film scaffolds subcutaneously in rats to test the biocompatibility and host response at time points of week 1, week 2, and week 4. The in vitro studies revealed that the initial monocyte adhesion on the aligned fiber scaffold was significantly less (p < 0.001) when compared to the random fiber scaffold. The in vivo study showed that the thicknesses of fibrous capsule on fibrous scaffolds were 7.55 +/- 0.54 microm for aligned fibers and 4.13 +/- 0.31 microm for random fibers, which were significantly thinner than that of film implants 37.7 +/- 0.25 microm (p < 0.001). Additionally, cell infiltration was observed in aligned fibrous scaffolds both in vitro and in vivo, while on random fibers and films, distinct fibrous capsule boundaries were found on the surfaces. These results indicate that aligned electrospun nanofibers may serve as a promising scaffold for tissue engineering by minimizing host response, enhancing tissue-scaffold integration, and eliciting a thinner fibrous capsule.
Cao
Haoqing
H
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459.
McHugh
Kevin
K
Chew
Sing Yian
SY
Anderson
James M
JM
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D005260:Female; D005549:Foreign-Body Reaction / Q000473:pathology*; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D008422:Materials Testing; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
D011091:Polyesters; C016240:polycaprolactone