title: Parvo-like virus in the hepatopancreas of freshwater prawns Macrobrachium rosenbergii cultivated in Thailand.
abstract: A survey of cultivated giant freshwater prawns Macrobrachium rosenbergii from Thailand revealed the presence of unusual spherical to ovoid inclusions in nuclei of hepatopancreas tubule epithelial cells. These began as small eosinophilic inclusions that became more basophilic as they increased in size. They were present in both R-cells and E-cells but were largest and deeply basophilic only in the E-cells. Confocal laser microscopy revealed that stained nucleic acid fluorescence from the inclusions was lost by treatment with DNase I specific for double- and single-stranded DNA and also lost or reduced by treatment with mungbean nuclease specific for single-stranded nucleic acids. Transmission electron microscopy (TEM) revealed that the inclusions contained tightly packed, unenveloped, viral-like particles of approximately 25 to 30 nm diameter, resembling those produced by shrimp parvoviruses. However, PCR, in situ hybridization and immunohistochemical tests for shrimp parvoviruses previously reported from Thailand were all negative. These results suggested that the inclusions contained a parvo-like virus, not previously reported from M. rosenbergii in Thailand.
authors.affiliation: Centex Shrimp, Chalerm Prakiat Building, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.
authors.forename: Warachin
authors.initials: W
authors.lastname: Gangnonngiw
authors.forename: Wansika
authors.initials: W
authors.lastname: Kiatpathomchai
authors.forename: Siriporn
authors.initials: S
authors.lastname: Sriurairatana
authors.forename: Kesinee
authors.initials: K
authors.lastname: Laisutisan
authors.forename: Niti
authors.initials: N
authors.lastname: Chuchird
authors.forename: Chalor
authors.initials: C
authors.lastname: Limsuwan
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Flegel
mesh_terms: D000818:Animals; D017756:Aquaculture; D005618:Fresh Water; D043143:Hepatopancreas / Q000473:pathology / Q000821:virology; D046529:Microscopy, Electron, Transmission; D033541:Palaemonidae / Q000821:virology*; D017992:Parvovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D013785:Thailand