Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer.
We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.
Frye
Michaela
M
Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge CB2 1QR, UK.
Dragoni
Ilaria
I
Chin
Suet-Feung
SF
Spiteri
Immaculada
I
Kurowski
Agata
A
Provenzano
Elena
E
Green
Andrew
A
Ellis
Ian O
IO
Grimmer
Donna
D
Teschendorff
Andrew
A
Zouboulis
Christos C
CC
Caldas
Carlos
C
Watt
Fiona M
FM
D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics*; D002470:Cell Survival; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D012333:RNA, Messenger; D008780:Methyltransferases; C515610:NSUN2 protein, human