Stability of the animal model of oleic acid-induced acute lung injury.
OBJECTIVE
To evaluate the stability of hemodynamic, respiratory and gas exchange variables in an animal model of oleic acid-induced acute lung injury.


METHODS
This was an experimental study involving 10 mongrel dogs. The variables were measured at baseline, as well as at 30, 60, 90 and 120 min after the administration of oleic acid. In order to analyze repeated measurements, linear and quadratic effects were tested. Mixed linear models with diversified variance and covariance structures were used, depending on the variable studied.


RESULTS
We found that mean arterial blood pressure stabilized at 30 min, as did heart rate, pulmonary arterial pressure and pulmonary capillary pressure at 60 min. Respiratory rate, tidal volume, minute volume and respiratory work stabilized at 30 min. Regarding gas exchange variables, PaO2, PaO2/FiO2 ratio and pulmonary shunt fraction stabilized at 30 min. The remaining variables maintained a continuous rise or fall.


CONCLUSIONS
This oleic acid-induced acute lung injury model is stable for some of the variables tested, although stabilization occurs at different times. The respiratory and gas exchange variables stabilized at 30 min, whereas the hemodynamic variables stabilized at 60 min.
Gaio
Eduardo
E
Departamento de Clínica Médica, Universidade de Brasília, Brasília, DF, Brasil. eduardogaio@unb.br
Melo e Silva
César Augusto de
CA
Brito
Flávio
F
Firmino
Marco Aurélio Pereira
MA
Storck
Rodrigo
R
Freitas
Eduardo
E
D055371:Acute Lung Injury / Q000139:chemically induced / Q000503:physiopathology*; D000704:Analysis of Variance; D000818:Animals; D004285:Dogs; D006439:Hemodynamics / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D019301:Oleic Acid; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D015656:Respiratory Mechanics / Q000502:physiology*; D013997:Time Factors
D019301:Oleic Acid