title: Multipyrene tandem probes for detection of C677T polymorphism in MTHFR gene.
abstract: We designed tandems of oligo(2'-O-methylribonucleotides) conjugates containing two bispyrene (5'-bisPyr and 3'-bisPyr) groups on their junction for detection of C677T polymorphism in the methylenetetrahydrofolate reductase gene (MHTFR). The potential of SNP detection with multipyrene tandems of oligo(2'-O-methylribonucleotides) was demonstrated.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk 630090, Russia. danov@niboch.nsc.ru
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Kholodar
authors.forename: Daria S
authors.initials: DS
authors.lastname: Novopashina
authors.forename: Mariya I
authors.initials: MI
authors.lastname: Meschaninova
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Lomzov
authors.forename: Alya G
authors.initials: AG
authors.lastname: Venyaminova
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D042965:Methylenetetrahydrofolate Reductase (NADPH2)