Influence of size on apparent scrambling of sequence during CID of b-type ions.
We investigated the influence of peptide size on the apparent loss of sequence during collision-induced dissociation (CID) of b ions using a group of peptides containing from between 4 and 10 residues. Although scrambling of sequence for b3+ generated from tetrapeptides is minimal, significant formation of nondirect sequence ions (i.e., ions for which scrambling has apparently occurred) was observed for all larger b ions included in the study.
Molesworth
Samuel
S
Department of Chemistry, Wichita State University, Wichita, Kansas 67260-0051, USA.
Osburn
Sandra
S
Van Stipdonk
Michael
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D007477:Ions / Q000145:classification*; D007536:Isomerism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D007477:Ions; D009842:Oligopeptides