A Tucker model based approach for analysis of complex oil biodegradation data.
A novel method based on gas chromatography-mass spectrometry in selected ion monitoring mode (GC-MS/SIM) and Tucker models is developed to evaluate the effects of oil type, microbial treatments and incubation time on the biodegradation of petroleum hydrocarbons. The data set consists of sections of the m/z 180, 192 and 198 GC-MS/SIM chromatograms of oil extracts from a biodegradation experiment where four oil types were exposed to four microbial treatments over a period of one year. The chosen sections, which are specific to methylfluorenes, phenanthrenes and dibenzothiophenes, were combined in a 4-way array (incubation timexoil typextreatmentxcombined chromatographic retention times) that was analyzed using both principal component analysis and the Tucker model. Several conclusions could be reached: the light fuel oil was the least degradable of those tested, 2- and 3-methyl isomers were more easily degraded compared to the 4-methyl isomers, the mixture of surfactant producers and PAC degraders provided the most effective degradation and the largest part of the degradation occurred between 54 and 132 days.
Tomasi
Giorgio
G
Department of Basic Sciences and Environment, The Faculty of Life Sciences, Copenhagen University, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark. gto@life.ku.dk
Christensen
Jan H
JH
D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006838:Hydrocarbons / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D010578:Petroleum / Q000032:analysis* / Q000378:metabolism
D006838:Hydrocarbons; D010578:Petroleum