title: Comparison of the oxidative status of vitamin E-supplemented and non-supplemented cows under field conditions.
abstract: The effect of oral vitamin E supplementation during the dry period on serum biomarkers of oxidative status was evaluated in an observational field study. Ninety-eight cows were selected from 26 herds in the Netherlands. On 15 farms dry cows were supplemented with a dry-cow mineral mix containing 1000 IU vitamin E (vitamin E+ group, n = 52), and on 11 other farms dry cows did not receive the supplement (control group, n = 48). A blood sample was taken from all cows between 0 and 28 days before calving. Serum levels of alpha-tocopherol, cholesterol, triacylglycerol, superoxide dismutase (SOD), glutathione peroxidase (GSHpx), uric acid, albumin, protein sulphydryl oxidation level, reactive oxygen metabolites (ROM), iron, the ferric reducing ability (FRAP), and malondialdehyde (MDA) were measured. Alpha-tocopherol levels were significantly higher in the vitamin E+ group, as was the alpha-tocopherol:cholesterol ratio. MDA levels were significantly lower in the vitamin E+ group, suggesting that levels of lipid peroxidation were lower with vitamin E supplementation. In conclusion, vitamin E supplementation increases alpha-tocopherol and lowers MDA serum levels.
authors.affiliation: Departement Gezondheidszorg Landbouwhuisdieren, faculteit Diergeneeskunde, Utrecht, Nederland. R.J.Bouwstra@uu.nl
authors.forename: R J
authors.initials: RJ
authors.lastname: Bouwstra
authors.forename: M
authors.initials: M
authors.lastname: Nielen
authors.forename: T
authors.initials: T
authors.lastname: van Werven
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002417:Cattle / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D019587:Dietary Supplements; D005260:Female; D006304:Health Status; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D014810:Vitamin E / Q000008:administration & dosage*; D024502:alpha-Tocopherol / Q000097:blood*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D008315:Malondialdehyde; D002784:Cholesterol; D024502:alpha-Tocopherol