title: In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.
abstract: Matrix metalloproteases (MMPs) are endopeptidases involved in tumor cell invasion. Childhood acute lymphoblastic leukemia (ALL) is characterized by its capacity to infiltrate different organs. We analyzed the expression of MMP-2, -9, -14 and TIMP-1 and -2 in a prospective study on 86 children with newly diagnosed ALL (73 B- and 13 T-lineage) and 9 children at relapse with B-ALL. Membrane-bound and intracytoplasmic MMPs and TIMPs were analyzed by flow cytometry, and secreted MMPs were quantified by ELISA. In patients at relapse, MMP-14 was present in a greater proportion of the B-ALL cell population than at diagnosis. In patients with peripheral infiltration, intracytoplasmic MMP-9 was significantly higher than in patients without infiltration. ROC curve and Kaplan-Meier curve analysis showed that a high secretion of MMP-9 (>2450 pg/ml/10(6) cells) was associated with a lower overall survival rate, suggesting that the secretion of MMP-9 is an independent prognostic factor in childhood B-ALL.
authors.affiliation: Pediatric Hematology, Oncology and IHU, Rouen University Hospital, Rouen, France. pascale.schneider@chu-rouen.fr
authors.forename: Pascale
authors.initials: P
authors.lastname: Schneider
authors.forename: Odile
authors.initials: O
authors.lastname: Costa
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Legrand
authors.forename: Dany
authors.initials: D
authors.lastname: Bigot
authors.forename: Sylviane
authors.initials: S
authors.lastname: Lecleire
authors.forename: Vanessa
authors.initials: V
authors.lastname: Grassi
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Vannier
authors.forename: Marc
authors.initials: M
authors.lastname: Vasse
mesh_terms: D000293:Adolescent; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007621:Karyotyping; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D011446:Prospective Studies
chemical_list: D014408:Biomarkers, Tumor; D020780:Matrix Metalloproteinase 9