Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis.
Western blot analysis is a common technique for detecting a target protein on a nitrocellulose membrane. The target protein is detected using specific antibodies. The positive signal can be visualized either by chemiluminescence or by a colorimetric reaction. Some specific antibodies can be produced in-house in high amounts and can be used without considering costs. However many antibodies (especially antibodies for detection tag fused proteins) must be purchased from a company. Unfortunately most of them are very expensive. Here we report the effectiveness of different antibodies after reusing them several times and also compare the influence of different storage conditions.
Boonrod
K
K
RLP AgroScience GmbH, Alplanta, Institute for Plant Research, Breitenweg 71, D-67435 Neustadt, Germany. kajohn.boonrod@agroscience.rlp.de
Roth
B
B
Leong Ngar
S
S
Krczal
G
G
D000906:Antibodies / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods*; D055550:Protein Stability; D013696:Temperature
D000906:Antibodies