Tracking epitope-specific antiviral CD4+ T cell responses to a live attenuated vaccine reveals ongoing functional responses.
There are few studies that have examined the frequencies of epitope-specific CD4(+) T cells following the use of a highly effective vaccine, yet such data would potentially be of value for the development of novel vaccination strategies. In this study we tracked human epitope-specific CD4(+) T cell responses over time after immunisation with a live attenuated varicella zoster virus vaccine by MHC Class II tetrameric complexes and functional assays. We show that the peptide-specific responses reflect those against whole virus antigens, and are similar in both frequency and phenotype to those found in healthy volunteers, despite a highly attenuated and clinically inapparent infection.
Jones
L
L
MRC Human Immunology Unit, University of Oxford, Weatherall Institute of Molecular Medicine, Oxford, United Kingdom. louise.jones@imm.ox.ac.uk
Malavige
G
G
Jeffery
K
K
Kemp
E
E
Breuer
J
J
Klenerman
P
P
Ogg
G S
GS
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002644:Chickenpox / Q000517:prevention & control*; D019433:Chickenpox Vaccine / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D059811:HLA-DRB1 Chains; D006282:Health Personnel; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014613:Vaccines, Attenuated / Q000276:immunology; D055815:Young Adult
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019433:Chickenpox Vaccine; D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C489631:HLA-DRB1*15:01 antigen; D007074:Immunoglobulin G; D014613:Vaccines, Attenuated; D007371:Interferon-gamma