title: [Effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells].
abstract: OBJECTIVE
  To investigate the effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells.
  
  
  METHODS
  The proliferation inhibitory rate was evaluated by MTT assay. The telomerase activity was analyzed by TRAP-ELISA, and the expression of hTERT mRNA was determined by semi-quantitative RT-PCR.
  
  
  RESULTS
  Matrine displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against SW1116 cells. Compared with control group, the telomerase activity and the expression of hTERT mRNA decreased significantly (P < 0.05 or P < 0.01) in matrine group.
  
  
  CONCLUSIONS
  Matrine can inhibit the telomerase activity by depressing the expression of hTERT in SW1116 cells and inhibiting cell proliferation.
authors.affiliation: Department of Gastroenterology, Youjiang Medical College for Nationalities, Baise 533000, China. ZHXH@ymcn.gx.cn
authors.forename: Xi-Han
authors.initials: XH
authors.lastname: Zhou
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Zan-Song
authors.initials: ZS
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Cen
authors.forename: Yi-Xia
authors.initials: YX
authors.lastname: Yin
authors.forename: Yue-Qiu
authors.initials: YQ
authors.lastname: Qin
authors.forename: Qian-Bo
authors.initials: QB
authors.lastname: Su
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D011807:Quinolizines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029909:Sophora / Q000737:chemistry*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D011807:Quinolizines; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase; D000093842:Matrines