Lipidomic analysis of biological samples by liquid chromatography coupled to mass spectrometry.
Lipidomics studies the large-scale changes in nonwater-soluble metabolites (lipids) accompanying perturbations of biological systems. Because lipids are involved in crucial biological mechanisms, there is a growing scientific interest in using lipidomic approaches to understand the regulation of the lipid metabolism in all eukaryotic and prokaryotic organisms. Lipidomics is a powerful tool in system biology that can be used together with genomics, transcriptomics, and proteomics to answer biological questions arising from various scientific areas such as environmental sciences, pharmacology, nutrition, biophysics, cell biology, physiology, pathology, and disease diagnostics. One of the main challenges for lipidomic analysis is the range of concentrations and chemical complexity of different lipid species. In this chapter, we present a lipidomic approach that combines sample preparation, chromatographic, and intrasource ionization separation coupled to mass spectrometry for analyzing a broad-range of lipid molecules in biological samples.
Astarita
Giuseppe
G
Department of Pharmacology, University of California-Irvine, Irvine, CA, USA.
Ahmed
Faizy
F
Piomelli
Daniele
D
D002853:Chromatography, Liquid / Q000379:methods*; D003933:Diagnosis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards