[Construction of mammalian cell lines continuously expressing JEV structural proteins].
Based on the infectious clone of JEV vaccine strain SA14-14-2, prM-E genes and C-prM-E genes were cloned into pCDNA3.1 vector. The recombinant plasmid pCJE-ME was transfected into BHK-21 cells, the expressed proteins were toxic to the cell growth and accelerated the cell death. But when transfected with the plasmid pCJE-CME, the cell lines continuously expressing structural proteins could be selected with G418. And the expression products of pCJE-CME vector could be detected by ELISA, Western Blot and IFA assay. It showed that the JEV capsid protein could enhance the stability of the cell lines expressing the structural proteins. The established cell lines can make the acquirement of the virus-like particles much easier.
Liu
Shan
S
Lab for Protein Engineering, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Huang
Ying
Y
Yang
Peng
P
Sun
Zhi-Wei
ZW
Yu
Wei-Yuan
WY
D000818:Animals; D017209:Apoptosis; D016466:CHO Cells; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line / Q000166:cytology / Q000821:virology*; D006224:Cricetinae; D003412:Cricetulus; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism
D036022:Capsid Proteins; D015678:Viral Structural Proteins