[HPLC fingerprint of flavonoids and phenols of Dendrobium nobile].
OBJECTIVE
To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile.


METHODS
Phenomenex prodigy ODS(3) C18 column (250 mm x 4.6 mm, 5 microm) was used with a mixture of acetonitrile-0.1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25 degrees C, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm.


RESULTS
The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed.


CONCLUSIONS
This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.
Ou
Huan-Jiao
HJ
Guangzhou University of Chinese Medicine, Guangzhou 510006, China. ouhuanjiao@163.com
Cheng
Jin-Le
JL
Li
Xian-Xia
XX
Zhan
Ruo-Ting
RT
Liang
Yi-Zeng
YZ
Xu
Ji-Yin
JY
Xu
Hui
H
Yan
Ping
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031667:Dendrobium / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010636:Phenols; D012997:Solvents