Vector-based siRNA delivery strategies for high-throughput screening of novel target genes.
Application of siRNA in high-throughput fashion is still in its early phase although the principle has been established for three years. In this review, we outline the different vector-based siRNA delivery platforms as well as resources that are becoming available for high-throughput applications, and some initial outcomes of vector siRNA high-throughput screening efforts using vector encoded siRNA. It is expected that further improvement of the siRNA technology and availability of the siRNA resources will help to materialize the potential of siRNA for functional genomics and drug target validation.
Chen
Meihong
M
Chinese Human Genome Center North, Beijing, 100176, China and Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology, Beijing, 100005, P. R. China.
Du
Quan
Q
Zhang
Hong-Yan
HY
Wahlestedt
Claes
C
Liang
Zicai
Z