Development of cerebral fiber pathways in cats revealed by diffusion spectrum imaging.
Examination of the three-dimensional axonal pathways in the developing brain is key to understanding the formation of cerebral connectivity. By tracing fiber pathways throughout the entire brain, diffusion tractography provides information that cannot be achieved by conventional anatomical MR imaging or histology. However, standard diffusion tractography (based on diffusion tensor imaging, or DTI) tends to terminate in brain areas with low water diffusivity, indexed by low diffusion fractional anisotropy (FA), which can be caused by crossing fibers as well as fibers with less myelin. For this reason, DTI tractography is not effective for delineating the structural changes that occur in the developing brain, where the process of myelination is incomplete, and where crossing fibers exist in greater numbers than in the adult brain. Unlike DTI, diffusion spectrum imaging (DSI) can define multiple directions of water diffusivity; as such, diffusion tractography based on DSI provides marked flexibility for delineation of fiber tracts in areas where the fiber architecture is complex and multidirectional, even in areas of low FA. In this study, we showed that FA values were lower in the white matter of newborn (postnatal day 0; P0) cat brains than in the white matter of infant (P35) and juvenile (P100) cat brains. These results correlated well with histological myelin stains of the white matter: the newborn kitten brain has much less myelin than that found in cat brains at later stages of development. Using DSI tractography, we successfully identified structural changes in thalamo-cortical and cortico-cortical association tracts in cat brains from one stage of development to another. In newborns, the main body of the thalamo-cortical tract was smooth, and fibers branching from it were almost straight, while the main body became more complex and branching fibers became curved reflecting gyrification in the older cats. Cortico-cortical tracts in the temporal lobe were smooth in newborns, and they formed a sharper angle in the later stages of development. The cingulum bundle and superior longitudinal fasciculus became more visible with time. Within the first month after birth, structural changes occurred in these tracts that coincided with the formation of the gyri. These results show that DSI tractography has the potential for mapping morphological changes in low FA areas associated with growth and development. The technique may also be applicable to the study of other forms of brain plasticity, including future studies in vivo.
Takahashi
Emi
E
Department of Radiology, Harvard Medical School, Charlestown, MA, USA. emi@nmr.mgh.harvard.edu
Dai
Guangping
G
Wang
Ruopeng
R
Ohki
Kenichi
K
Rosen
Glenn D
GD
Galaburda
Albert M
AM
Grant
P Ellen
PE
Wedeen
Van J
VJ
D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D016880:Anisotropy; D048588:Benzoxazines; D002415:Cats; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods; D007211:Indoles; D009186:Myelin Sheath / Q000502:physiology; D009413:Nerve Fibers, Myelinated / Q000502:physiology; D009434:Neural Pathways / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology; D010078:Oxazines; D013788:Thalamus / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology
D048588:Benzoxazines; D007211:Indoles; D010078:Oxazines; C018588:Luxol Fast Blue MBS; C028911:cresyl violet