Limit of detection of sedimentation and counting technique (SCT) for Echinococcus multilocularis diagnosis, estimated under experimental conditions.
The aim of this study was to estimate the limit of detection of sedimentation and counting techniques (SCT) in Echinococcus multilocularis diagnosis. Samples of small intestines, experimentally enriched with known numbers of E. multilocularis tapeworms, were used. Forty intestinal samples containing 2, 5, 10, and 30 E. multilocularis tapeworms (10 samples for each level) were prepared and examined according to SCT. E. multilocularis was detected in 30%, 40%, 60%, and 100% in samples enriched with 2, 5, 10, and 30 tapeworms, respectively. The limit of detection was estimated at 10 E. multilocularis tapeworms per sample of intestine (for 60% probability of obtaining positive results). There was a wide dispersion of counting results; these were observed in samples containing the same numbers of tapeworms, which indicates the low repeatability of the method. The limitations of SCT determined in this experiment should be considered when analysing the prevalence of E. multilocularis in carnivores.
Karamon
Jacek
J
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute in Pulawy, Al Partyzantow 57, 24-100 Pulawy, Poland. J.Karamon@piwet.pulawy.pl
Sroka
Jacek
J
Cencek
Tomasz
T
D000818:Animals; D004443:Echinococcosis / Q000175:diagnosis* / Q000469:parasitology; D048210:Echinococcus multilocularis / Q000254:growth & development / Q000302:isolation & purification*; D005589:Foxes; D007422:Intestines / Q000469:parasitology*; D010274:Parasitology / Q000379:methods* / Q000592:standards