Inhibition of influenza virus by baculovirus-mediated shRNA.
Influenza viruses A and B cause widespread infections of the human respiratory tract; however, existing vaccines and drug therapy are of limited value for their treatment. Here we show that bispecific short hairpin small-interfering RNA constructs containing an eight-nucleotide intervening spacer, targeted against influenza virus A or influenza virus B, can inhibit the production of both types of virus in infected cell lines. This multiple vector showed remarkable ability to cope with both influenza virus A or B. Furthermore, the Autographa californica multiple nuclear polyhedrosis virus can infect a range of mammalian cells, facilitating its use as a baculovirus vector for gene delivery into cells. In this study, baculovirus-mediated bispecific short-hairpin RNA expression markedly inhibited both influenza virus A and B production.
Suzuki
Hitoshi
H
Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology and High Technology Research Center, Chiba Institute of Technology, Narashino Chiba 275-0016, Japan.
Saitoh
Hiroshi
H
Suzuki
Tomoyuki
T
Takaku
Hiroshi
H
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D004285:Dogs; D015536:Down-Regulation; D005822:Genetic Vectors; D009980:Influenza A virus / Q000235:genetics*; D009981:Influenza B virus / Q000235:genetics*; D019590:Nucleocapsid Proteins; D034741:RNA, Small Interfering / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D014758:Viral Core Proteins / Q000037:antagonists & inhibitors / Q000235:genetics
C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins