title: Postoperative intra-abdominal infection increases angiogenesis and tumor recurrence after surgical excision of colon cancer in mice.
abstract: BACKGROUND
  Recent reports have suggested that anastomotic leakage is associated with greater rates of tumor recurrence and cancer-specific mortality after surgery for colorectal cancer. The impact of postoperative intra-abdominal infection on long-term oncologic results, however, is still controversial, and no direct causal relationship has been found between both processes. Our aim was to investigate the influence of postoperative intraabdominal infection on angiogenesis and tumor growth in an animal model of colon cancer.
  
  
  METHODS
  Balb/c mice were randomized immediately after injection of 5x10(6) B51LiM cells into the cecal wall into 2 groups: cecal resection without postoperative infection (group 1), and cecal resection with postoperative intra-abdominal infection (group 2). A total of 18 days after cell injection, cecectomy was performed, and infection was induced in group 2 by intraperitoneal injection of 3x10(8) colony-forming units of Bacteroides fragilis. On postoperative day 12, the mice were killed.
  
  
  RESULTS
  Comparing group 1 with group 2, tumor recurrence was more frequent in animals with intraabdominal infection (65% vs 100%, respectively; P=02). VEGF serum levels were greater at the time of sacrifice in the group with infection (11+/-10 vs 30+/-23 pg/mL; P<.05). Tumor angiogenesis was also increased in the postoperative infection group. The mean (+/-standard deviation) microvessel density was 16+/-7 versus 28+/-11 vessels per high-power field (P<.05).
  
  
  CONCLUSIONS
  We concluded that postoperative intra-abdominal infection increases angiogenesis and tumor recurrence after operative excision of a colon cancer in mice.
authors.affiliation: Colorectal Surgery Unit, Department of Surgery, Hospital del Mar, Barcelona, Spain.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Bohle
authors.forename: Miguel
authors.initials: M
authors.lastname: Pera
authors.forename: Marta
authors.initials: M
authors.lastname: Pascual
authors.forename: Sandra
authors.initials: S
authors.lastname: Alonso
authors.forename: Xavier
authors.initials: X
authors.lastname: Mayol
authors.forename: Margarita
authors.initials: M
authors.lastname: Salvado
authors.forename: Jan
authors.initials: J
authors.lastname: Schmidt
authors.forename: Luis
authors.initials: L
authors.lastname: Grande
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000601:surgery*; D000818:Animals; D045744:Cell Line, Tumor; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology / Q000601:surgery*; D051379:Mice; D008807:Mice, Inbred BALB C; D009364:Neoplasm Recurrence, Local / Q000209:etiology*; D009389:Neovascularization, Pathologic / Q000209:etiology*; D010538:Peritonitis / Q000150:complications; D013530:Surgical Wound Infection / Q000150:complications*