Cloning and tissue-specific expression of a delta-COP homologue in a freshwater and a brackish water-adapted strain of rainbow trout (Oncorhynchus mykiss).
In eukaryotic cells, intracellular transport is mediated by coated vesicular carriers. Coat proteins I (COPI) vesicles are involved in the retrograde transport from Golgi apparatus to the endoplasmic reticulum. The COPI complex is composed of ADP-ribosylation factor 1 and coatomer comprising seven subunits, termed alpha-zeta. We isolated and characterised a cDNA sequence from rainbow trout homolog to delta-subunit of COPI complex (delta-COP). Trout delta-COP gene encodes a protein of 509 aa including a characteristic Mu homology domain. Searches at the Ensemble Genome browser identified three additional teleostean delta-COP-like sequences from pufferfish, rice fish, and stickleback. Sequence identity of piscine delta-COP protein sequences is greater than 84%. Moreover, a phylogenetic analysis indicates that delta-COP protein sequences are strongly conserved among vertebrate species. delta-COP homologue is ubiquitously expressed in trout tissues. Quantitative Real-Time RT-PCR revealed that delta-COP is differentially expressed in liver and gill tissue of two rainbow trout strains, the freshwater strain STEELHEAD and the brackish water-adapted strain BORN.
Rebl
Alexander
A
Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Molecular Biology Research Unit, Dummerstorf, Germany. rebl@fbn-dummerstorf.de
Anders
Eckhard
E
Wimmers
Klaus
K
Goldammer
Tom
T
D000220:Adaptation, Biological / Q000235:genetics*; D000818:Animals; D001454:Baltic States; D003001:Cloning, Molecular; D020756:Coatomer Protein / Q000235:genetics* / Q000378:metabolism*; D005618:Fresh Water; D005880:Gills / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D012623:Seawater; D017385:Sequence Homology; D014018:Tissue Distribution / Q000235:genetics
D020756:Coatomer Protein