Mass spectrometry-based metabolomics oriented by correlation analysis for wound-induced molecule discovery: identification of a novel jasmonate glucoside.
BACKGROUND
Jasmonates are members of the oxylipin family involved in various plant regulatory processes, particularly in the response to biotic and abiotic stresses. They are present in very low amounts in wounded plants, which complicates their detection within complex plant extracts. Therefore, advanced analytical methods are needed for the profiling and characterisation of novel jasmonate derivatives.


OBJECTIVE
To use metabolomics to search for original wound-induced metabolites belonging to the jasmonate family.


METHODS
Numerous Arabidopsis specimens harvested at various time points after wounding were analysed by ultra-high pressure liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC/TOFMS). A correlation analysis based on kinetic profile similarities with known jasmonates was applied to find wound-induced molecules having a potential role in defence signalling. Their characterisation was performed by tandem mass spectrometry and capillary nuclear magnetic resonance spectroscopy.


RESULTS
The statistical data treatment highlighted several previously reported jasmonates as well as a new glucoside derivative of the jasmonate 3-oxo-2-(2Z-pentenyl) cyclopentane-1-butyric acid (OPC-4). The monitoring of its kinetics in response to wounding revealed a delayed accumulation compared to the profile of OPC-4. This suggests an inactivation or elimination of OPC-4 through the formation of a polar glucosylated metabolite.


CONCLUSIONS
The metabolomic approach developed has proved useful in the discovery of original jasmonates synthesised in response to plant wounding.
Glauser
Gaetan
G
School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30, quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
Boccard
Julien
J
Rudaz
Serge
S
Wolfender
Jean-Luc
JL
D017360:Arabidopsis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000032:analysis / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics; D019906:Nuclear Magnetic Resonance, Biomolecular; D054883:Oxylipins / Q000032:analysis / Q000378:metabolism*
D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid