title: Multilayer fluorescence optically encoded beads for protein detection.
abstract: An easy preparation method of multilayer fluorescence optically encoded beads for protein detection is presented. The beads, which consist of multicolored layers, are made from amino polyethylene glycol grafted polystyrene (PS-g-PEG) beads by using several fluorescent dyes such as fluorescein isothiocyanate (FITC) and rhodamine via controlling diffusion of an Fmoc-protecting group after HCl solution swelling. A biotin, glutathione S-transferase (GST) antibody, and an RNA aptamer that specifically recognize streptavidin, GST antigen, and hepatitis C virus (HCV) helicase are introduced to the optically encoded beads and monitored for their binding activity to the target molecules. After binding, the ligands are identified easily by their color codes.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.forename: Chul
authors.initials: C
authors.lastname: Rho
authors.forename: Jang-Woong
authors.initials: JW
authors.lastname: Byun
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Chung
authors.forename: Homan
authors.initials: H
authors.lastname: Kang
authors.forename: Juyoung
authors.initials: J
authors.lastname: Park
authors.forename: Su-Hyung
authors.initials: SH
authors.lastname: Cho
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D020365:RNA Helicases / Q000032:analysis; D012235:Rhodamines / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011137:Polystyrenes; D011506:Proteins; D012235:Rhodamines; C103390:polyethylene glycol-polystyrene; D011092:Polyethylene Glycols; D001710:Biotin; D019809:Streptavidin; D005982:Glutathione Transferase; D020365:RNA Helicases; D016650:Fluorescein-5-isothiocyanate