Development of a novel device to trap heavy metal cations: application of the specific interaction between heavy metal cation and mismatch DNA base pair.
We have already found that Hg(II) cation specifically binds to T:T mismatch base pair in heteroduplex DNA, which increases the melting temperature of heteroduplex DNA involving T:T mismatch base pair by about 4 degrees C. We have also found that Ag(I) cation specifically binds to C:C mismatch base pair in heteroduplex DNA, which increases the melting temperature of heteroduplex DNA involving C:C mismatch base pair by about 4 degrees C. Using the specific interaction, we developed a novel device to trap each of Hg(II) and Ag(I) cation. The device is composed of 5'-biotinylated T-rich or C-rich DNA oligonucleotides, BIO-T20: 5'-Bio-T(20)-3' or BIO-C20: 5'-Bio-C(20)-3' (Bio is a biotin), immobilized on streptavidin-coated polystylene beads. When the BIO-T20-immobilized beads were added to a solution containing Hg(II) cation, and the beads trapping Hg(II) cation were collected by centrifugation, almost all of Hg(II) cation were removed from the solution. Also, when the BIO-C20-immobilized beads were added to a solution containing Ag(I) cation, and the beads trapping Ag(I) cation were collected by centrifugation, almost all of Ag(I) cation were removed from the solution. We conclude that, using the novel device developed in this study, Hg(II) and Ag(I) cation can be effectively removed from the solution.
Torigoe
Hidetaka
H
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, Tokyo 162-8601, Japan. htorigoe@rs.kagu.tus.ac.jp
Miyakawa
Yukako
Y
Fukushi
Miyako
M
Ono
Akira
A
Kozasa
Tetsuo
T
D020137:Base Pair Mismatch; D019921:Biotinylation; D002412:Cations / Q000737:chemistry; D008628:Mercury / Q000737:chemistry* / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012834:Silver / Q000737:chemistry* / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence
D002412:Cations; D009838:Oligodeoxyribonucleotides; D011137:Polystyrenes; D012834:Silver; D008628:Mercury