title: Large scale study of epidermal recovery after stratum corneum removal: dynamics of genomic response.
abstract: The stratum corneum (SC) is a superficial skin compartment that protects the body from the outside environment. Any disturbance of this function induces cascading steps of molecular and cellular repair in the whole epidermis. The aim of this study was to investigate epidermal gene expression following SC removal by tape stripping. Twenty-nine healthy male volunteers were included (27 +/- 4 years old). Tape stripping was processed on one inner forearm, the other unstripped forearm served as a control. Epidermis samples were collected at 2, 6, 19, 30 and 72 h after tape stripping. Trans-epidermal water loss measurements were performed at each step to monitor barrier restoration. Total RNA was extracted from collected epidermis samples and analysed by using DermArray cDNA microarrays. Among 4000 genes under investigation, we found that the expression of 370 genes varied significantly at least once during the time following stripping. Using an original clustering method, the modulated genes were gathered into eight groups. A functional characterization of the clusters enabled us to get a dynamic and global view of the main molecular processes taking place during epidermal recovery.
authors.affiliation: L'Oréal Recherche, Clichy, France.
authors.forename: Peggy
authors.initials: P
authors.lastname: Sextius
authors.forename: Claire
authors.initials: C
authors.lastname: Marionnet
authors.forename: Francois-Xavier
authors.initials: FX
authors.lastname: Bon
authors.forename: Albane Lamy
authors.initials: AL
authors.lastname: de La Chapelle
authors.forename: Charlotte
authors.initials: C
authors.lastname: Tacheau
authors.forename: Morad
authors.initials: M
authors.lastname: Lahfa
authors.forename: Alain
authors.initials: A
authors.lastname: Mauviel
authors.forename: Bruno A
authors.initials: BA
authors.lastname: Bernard
authors.forename: Jacques
authors.initials: J
authors.lastname: Leclaire
authors.forename: Francoise
authors.initials: F
authors.lastname: Bernerd
authors.forename: Louis
authors.initials: L
authors.lastname: Dubertret
mesh_terms: D000328:Adult; D030541:Databases, Genetic; D004817:Epidermis / Q000293:injuries* / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D007700:Kinetics; D008297:Male; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014945:Wound Healing / Q000235:genetics*; D055815:Young Adult