title: Cold-active halophilic bacteria from the ice-sealed Lake Vida, Antarctica.
abstract: Lake Vida is a large, permanently ice-covered lake in the Victoria Valley of the McMurdo Dry Valleys, Antarctica and is unique among Dry Valley lakes because it is ice-sealed, with an ice-cover of nearly 19 m. Enrichment cultures of melt-water from Lake Vida 15.9 m ice yielded five pure cultures of aerobic, heterotrophic bacteria. Of these, one strain grew at -8 degrees C and the four others at -4 degrees C. All isolates were either halotolerant or halophilic, with two strains capable of growth at 15% NaCl. Phylogenetic analysis revealed the Lake Vida isolates to be Gammaproteobacteria, related to species of Psychrobacter and Marinobacter. This is the first report of pure cultures of bacteria from Lake Vida, and the isolates displayed a phenotype consistent with life in a cold hypersaline environment.
authors.affiliation: Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA.
authors.forename: Lindsay J
authors.initials: LJ
authors.lastname: Mondino
authors.forename: Marie
authors.initials: M
authors.lastname: Asao
authors.forename: Michael T
authors.initials: MT
authors.lastname: Madigan
mesh_terms: D000864:Antarctic Regions; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D052836:Heterotrophic Processes; D046448:Ice Cover / Q000382:microbiology*; D010802:Phylogeny; D054712:Salinity; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial