A novel method to detect seven microcystins in hard clam and corbicula fluminea by liquid chromatography-tandem mass spectrometry.
A simple and reliable method to detect seven microcystins in hard clam and corbicula fluminea, based on liquid chromatography with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS), was developed and validated. The sample preparation procedure includes extraction of tissue by methanol, followed by cleanup on a reversed-phase solid phase extraction (SPE) cartridge. With the optimized method, recoveries were between 43.7% and 92.3% for hard clam, 54.3% and 93.8% for corbicula fluminea, the relative standard deviations (RSD) were less than or equal to 16.2% and 15.7% in hard clam and corbicula fluminea at spiking levels of 1 microg/kg, 2 microg/kg and 5 microg/kg for MC-RR, MC-YR, MC-LR, and MC-LY, and 2 microg/kg, 5 microg/kg and 10 microg/kg for MC-LA, MC-LW and MC-LF, respectively, the limits of quantitation (LOQ) of this method were ranged from 0.7 microg/kg to 2.0 microg/kg.
Yang
Bo
B
School of Science, Nanjing University of Technology, Nanjing 210009, PR China.
Xu
Jin-Zhong
JZ
Ding
Tao
T
Wu
Bin
B
Jing
Su
S
Ding
Shu-jing
SJ
Chen
Hui-Lan
HL
Sheng
Chong-Yu
CY
Jiang
Yuan
Y
D000818:Animals; D049872:Bivalvia / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D049874:Corbicula / Q000378:metabolism*; D052998:Microcystins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D052998:Microcystins