title: Activation of PPAR gamma induces profound multilocularization of adipocytes in adult mouse white adipose tissues.
abstract: We sought to determine the effects of activation of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on multilocularization of adipocytes in adult white adipose tissue (WAT). Male C57BL/6 normal, db/db, and ob/ob mice were treated with agonists of PPAR-gamma, PPAR-alpha, or beta(3)-adrenoceptor for 3 weeks. To distinguish multilocular adipocytes from unilocular adipocytes, whole- mounted adipose tissues were co-immunostained for perilipin and collagen IV. PPAR-gamma activation with rosiglitazone or pioglitazone induced a profound change of unilocular adipocytes into smaller, multilocular adipocytes in adult WAT in a time-dependent, dose-dependent, and reversible manner. PPAR-alpha activation with fenofibrate did not affect the number of locules or remodeling. db/db and ob/ob obese mice exhibited less multilocularization in response to PPAR-gamma activation compared to normal mice. Nevertheless, all adipocytes activated by PPAR-gamma contained a single nucleus regardless of locule number. Multilocular adipocytes induced by PPAR-gamma activation contained substantially increased mitochondrial content and enhanced expression of uncoupling protein-1, PPAR-gamma coactivator-1-alpha, and perilipin. Taken together, PPAR-gamma activation induces profound multilocularization and enhanced mitochondrial biogenesis in the adipocytes of adult WAT. These changes may affect the overall function of WAT.
authors.affiliation: National Research Laboratory of Vascular Biology and Graduate School of Medical Science and Engineering, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Koh
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Park
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Jinah
authors.initials: J
authors.lastname: Han
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Lee
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Gou Young
authors.initials: GY
authors.lastname: Koh
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D052436:Adipose Tissue, White / Q000166:cytology*; D058667:Adrenergic beta-3 Receptor Agonists; D000818:Animals; D002352:Carrier Proteins; D048750:Cell Nucleus Division; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D047493:PPAR alpha / Q000819:agonists / Q000378:metabolism; D047495:PPAR gamma / Q000819:agonists* / Q000378:metabolism*; D000070778:Perilipin-1; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010750:Phosphoproteins / Q000378:metabolism; D000077205:Pioglitazone; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors; D000071256:Uncoupling Protein 1
chemical_list: D058667:Adrenergic beta-3 Receptor Agonists; D002352:Carrier Proteins; D007004:Hypoglycemic Agents; D007473:Ion Channels; D024101:Mitochondrial Proteins; D047493:PPAR alpha; D047495:PPAR gamma; D000070778:Perilipin-1; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010750:Phosphoproteins; C485290:Ppargc1a protein, mouse; D045162:Thiazolidinediones; D015534:Trans-Activators; D014157:Transcription Factors; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; D000077154:Rosiglitazone; D000077205:Pioglitazone