title: Systematic comparative protein expression profiling of clear cell renal cell carcinoma: a pilot study based on the separation of tissue specimens by two-dimensional gel electrophoresis.
abstract: Proteome-based technologies represent powerful tools for the analysis of protein expression profiles, including the identification of potential cancer candidate biomarkers. Thus, here we provide a comprehensive protein expression map for clear cell renal cell carcinoma established by systematic comparative two-dimensional gel electrophoresis-based protein expression profiling of 16 paired tissue systems comprising clear cell renal cell carcinoma lesions and corresponding tumor-adjacent renal epithelium using overlapping narrow pH gradients. This approach led to the mapping of 348 distinct spots corresponding to 248 different protein identities. By implementing restriction criteria concerning their detection frequency and overall regulation mode, 28 up- and 56 down-regulated single target spots were considered as potential candidate biomarkers. Based on their gene ontology information, these differentially expressed proteins were classified into distinct functional groups and according to their cellular distribution. Moreover, three representative members of this group, namely calbindin, gelsolin, and heart fatty acid-binding protein, were selected, and their expression pattern was analyzed by immunohistochemistry using tissue microarrays. Thus, this pilot study provides a significant update of the current renal cell carcinoma map and defines a number of differentially expressed proteins, but both their potential as candidate biomarkers and clinical relevance has to be further explored in tissues and for body fluids like serum and urine.
authors.affiliation: Institute of Medical Immunology, Martin Luther University Halle-Wittenberg, 06112 Halle/Saale, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Lichtenfels
authors.forename: Sven P
authors.initials: SP
authors.lastname: Dressler
authors.forename: Monica
authors.initials: M
authors.lastname: Zobawa
authors.forename: Christian V
authors.initials: CV
authors.lastname: Recktenwald
authors.forename: Angelika
authors.initials: A
authors.lastname: Ackermann
authors.forename: Derek
authors.initials: D
authors.lastname: Atkins
authors.forename: Michael
authors.initials: M
authors.lastname: Kersten
authors.forename: Andrea
authors.initials: A
authors.lastname: Hesse
authors.forename: Maria
authors.initials: M
authors.lastname: Puttkammer
authors.forename: Friedrich
authors.initials: F
authors.lastname: Lottspeich
authors.forename: Barbara
authors.initials: B
authors.lastname: Seliger
mesh_terms: D064026:Calbindins; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D018260:Gelsolin / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010865:Pilot Projects; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D046888:Tissue Array Analysis
chemical_list: D064026:Calbindins; C493408:FABP3 protein, human; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D018260:Gelsolin; D009363:Neoplasm Proteins; D064030:S100 Calcium Binding Protein G