title: Production of yeast (m2G10) methyltransferase (Trm11 and Trm112 complex) in a wheat germ cell-free translation system.
abstract: Transfer RNA (guanine-N(2)-)-methyltransferase [tRNA (m(2)G10) methyltransferase] catalyzes a methyl-transfer from S-adenosyl-L-methionine to N(2)-atom of guanine at position 10 (G10) in tRNA and generates N(2)-methylguanine at position 10 (m(2)G10). Yeast enzyme contains two protein subunits (Trm11 and Trm112). Trm11 protein is expected to be a catalytic subunit and Trm112 contains a Zinc-finger. In yeast cells, Trm112 binds not only to Trm11 but also to other proteins such as Lys9, Trm9, and Mtq2. Therefore, the Trm112 protein may regulate population of several protein complexes. To address these issues, we started the study on synthesis of Trm112 related protein complexes. In this meeting, we report synthesis of active Trm11-Trm112 complex in a wheat germ cell-free translation system.
authors.affiliation: Department of Materials Science and Biotechnology, Graduate School of Science and Engineering, Ehime University, Matsuyama 790-8577, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Okada
authors.forename: Yuki
authors.initials: Y
authors.lastname: Muneyoshi
authors.forename: Yaeta
authors.initials: Y
authors.lastname: Endo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hori
mesh_terms: D001483:Base Sequence; D002474:Cell-Free System; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D055503:Protein Multimerization; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D014908:Triticum / Q000235:genetics; D012359:tRNA Methyltransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D012359:tRNA Methyltransferases; C023196:tRNA (guanine(10)-N(2))-dimethyltransferase; C502204:Trm11 protein, S cerevisiae; C502205:Trm112 protein, S cerevisiae