title: Genotyping of 73 UM-SCC head and neck squamous cell carcinoma cell lines.
abstract: BACKGROUND
  We established multiple University of Michigan Squamous Cell Carcinoma (UM-SCC) cell lines. With time, these have been distributed to other labs all over the world. Recent scientific discussions have noted the need to confirm the origin and identity of cell lines in grant proposals and journal articles. We genotyped the UM-SCC cell lines in our collection to confirm their unique identity.
  
  
  METHODS
  Early-passage UM-SCC cell lines were genotyped and photographed.
  
  
  RESULTS
  Thus far, 73 unique head and neck UM-SCC cell lines (from 65 donors, including 21 lines from 17 females) were genotyped. In 7 cases, separate cell lines were established from the same donor.
  
  
  CONCLUSIONS
  These results will be posted on the UM Head and Neck SPORE Tissue Core website for other investigators to confirm that the UM-SCC cells used in their laboratories have the correct features. Publications using UM-SCC cell lines should confirm the genotype.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: J Chad
authors.initials: JC
authors.lastname: Brenner
authors.forename: Martin P
authors.initials: MP
authors.lastname: Graham
authors.forename: Bhavna
authors.initials: B
authors.lastname: Kumar
authors.forename: Lindsay M
authors.initials: LM
authors.lastname: Saunders
authors.forename: Robbi
authors.initials: R
authors.lastname: Kupfer
authors.forename: Robert H
authors.initials: RH
authors.lastname: Lyons
authors.forename: Carol R
authors.initials: CR
authors.lastname: Bradford
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Carey
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm