Handy and prompt DNA separation using PNA with internal disulfide bond.
PNA (peptide nucleic acid) is a DNA analogue which has a peptide backbone. PNA is very useful as a recognition probe because of its immensely high affinity to DNA. In this study, we used PNA as a DNA separation tool. Furthermore, we introduced a cleavable disulfide bond into main chain of PNA in order to facilitate the removal of PNA from PNA/DNA duplex. This disulfide bond was readily cleaved by various reducing agents and then the resultant two short PNA fragments could not form stable PNA/DNA duplex anymore. Accordingly, a desired DNA fragment was picked up from the DNA mixtures and readily recovered by the reductive treatment.
Aiba
Yuichiro
Y
Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.
Komiyama
Makoto
M
D019921:Biotinylation; D004247:DNA / Q000302:isolation & purification*; D004220:Disulfides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D004220:Disulfides; D020135:Peptide Nucleic Acids; D004247:DNA