Cellulase assays.
Cellulose is a heterogeneous polysaccharide, and its enzymatic hydrolysis requires endoglucanase, exoglucanase (cellobiohydrolase), and beta-glucosidase to work together. We summarize the most commonly used assays for individual enzymes and cellulase mixture.
Zhang
Y H Percival
YH
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Hong
Jiong
J
Ye
Xinhao
X
D000693:Anaerobiosis / Q000502:physiology; D002480:Cellulase / Q000032:analysis / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D004187:Disaccharides / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D006868:Hydrolysis; D001617:beta-Glucosidase / Q000378:metabolism
D004187:Disaccharides; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase