Peroxisome dynamics in Arabidopsis plants under oxidative stress induced by cadmium.
Peroxisomes are organelles with an essentially oxidative metabolism that are involved in various metabolic pathways such as fatty acid beta-oxidation, photorespiration, and metabolism of reactive oxygen species (ROS) and reactive nitrogen species. These organelles are highly dynamic but there is little information about the regulation of, and the effects of environment on, peroxisome movement. In this work a stable Arabidopsis line expressing the GFP-SKL peptide targeted to peroxisomes was characterized. Peroxisome-associated fluorescence was observed in all tissues, including leaves (mesophyll and epidermal cells, trichomes, and stomata) and roots. The dynamics of peroxisomes in epidermal cells was examined by confocal laser microscope, and various types of movement were observed. The speed of movement differed depending on the plant age. Treatment of plants with CdCl(2) (100 microM) produced a significant increase in speed, which was dependent on endogenous ROS and Ca(2+), but was not related to actin cytoskeleton modifications. In light of the results obtained, it is proposed that the increase in peroxisomal motility observed in Arabidopsis plants could be a cellular mechanism of protection against the Cd-imposed oxidative stress. Other possible roles for the enhanced peroxisome movement in plant cell physiology are discussed.
Rodríguez-Serrano
María
M
Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, 18080 Granada, Spain.
Romero-Puertas
María C
MC
Sparkes
Imogen
I
Hawes
Chris
C
del Río
Luis A
LA
Sandalio
Luisa M
LM
D017360:Arabidopsis / Q000502:physiology*; D019256:Cadmium Chloride / Q000494:pharmacology*; D048708:Cell Growth Processes / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D018613:Microscopy, Confocal; D009068:Movement / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D020675:Peroxisomes / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D019441:Plant Epidermis / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D018515:Plant Leaves / Q000166:cytology; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D019256:Cadmium Chloride