Co-localization of carbonic anhydrase and phosphoenol-pyruvate carboxylase and localization of pyruvate kinase in roots and hypocotyls of etiolated Glycine max seedlings.
We investigated the presence of carbonic anhydrase in root and hypocotyl of etiolated soybean using enzymatic, histochemical, immunohistochemical and in situ hybridization approaches. In parallel, we used in situ hybridization and immunolocalization to determine the expression pattern and localization of phosphoenolpyruvate carboxylase. Their co-localization in the root tip as well as in the central cylinder, suggests that a large fraction of the CO(2) may be re-introduced into C4 compounds. GmPK3 expression, coding for a cytoplasmic isoform of pyruvate kinase, was detected in all different root cell types, suggesting that both phosphoenolpyruvate-utilizing enzymes are involved in phosphoenolpyruvate metabolism in etiolated soybean roots; a case indicative of the necessary flexibility plant metabolism has to adopt in order to compensate various physiological conditions.
Dimou
Maria
M
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
Paunescu
Anca
A
Institute of Biology, Splaiul Independentei 296, Bucharest 060031, Romania.
Aivalakis
Georgios
G
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
Flemetakis
Emmanouil
E
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
Katinakis
Panagiotis
P
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D018546:Hypocotyl / Q000201:enzymology* / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology* / Q000378:metabolism; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology* / Q000378:metabolism
D010940:Plant Proteins; D011770:Pyruvate Kinase; D010730:Phosphoenolpyruvate Carboxylase; D002256:Carbonic Anhydrases