title: Polypyrimidine tract binding protein prevents activity of an intronic regulatory element that promotes usage of a composite 3'-terminal exon.
abstract: Alternative splicing of 3'-terminal exons plays a critical role in gene expression by producing mRNA with distinct 3'-untranslated regions that regulate their fate and their expression. The Xenopus alpha-tropomyosin pre-mRNA possesses a composite internal/3'-terminal exon (exon 9A9') that is differentially processed depending on the embryonic tissue. Exon 9A9' is repressed in non-muscle tissue by the polypyrimidine tract binding protein, whereas it is selected as a 3'-terminal or internal exon in myotomal cells and adult striated muscles, respectively. We report here the identification of an intronic regulatory element, designated the upstream terminal exon enhancer (UTE), that is required for the specific usage of exon 9A9' as a 3'-terminal exon in the myotome. We demonstrate that polypyrimidine tract binding protein prevents the activity of UTE in non-muscle cells, whereas a subclass of serine/arginine rich (SR) proteins promotes the selection of exon 9A9' in a UTE-dependent way. Morpholino-targeted blocking of UTE in the embryo strongly reduced the inclusion of exon 9A9' as a 3'-terminal exon in the endogenous mRNA, demonstrating the function of UTE under physiological circumstances. This strategy allowed us to reveal a splicing pathway that generates a mRNA with no in frame stop codon and whose steady-state level is translation-dependent. This result suggests that a non-stop decay mechanism participates in the strict control of the 3'-end processing of the alpha-tropomyosin pre-mRNA.
authors.affiliation: CNRS-Université de Rennes1 UMR 6061, Institut de Génétique et Développement de Rennes, IFR 140, Faculté de Médecine, CS 34317, 35043 Rennes Cedex, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Anquetil
authors.forename: Caroline
authors.initials: C
authors.lastname: Le Sommer
authors.forename: Agnès
authors.initials: A
authors.lastname: Méreau
authors.forename: Sandra
authors.initials: S
authors.lastname: Hamon
authors.forename: Hubert
authors.initials: H
authors.lastname: Lerivray
authors.forename: Serge
authors.initials: S
authors.lastname: Hardy
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001665:Binding Sites; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation; D007438:Introns; D008957:Models, Genetic; D009132:Muscles / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D026723:Polyadenylation; D038941:Polypyrimidine Tract-Binding Protein / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D014335:Tropomyosin / Q000737:chemistry; D014982:Xenopus laevis
chemical_list: D014335:Tropomyosin; D038941:Polypyrimidine Tract-Binding Protein; D012260:Ribonucleases