Gamma-delta T cell subsets are differentially associated with granuloma development and organization in a bovine model of mycobacterial disease.
The characteristic lesion in bovine tuberculosis is well-organized respiratory granulomas. This is typically associated with a strong T-helper 1 biased cell-mediated immune response and eventual containment of the infection. In bovine paratuberculosis, the classic lesion is unorganized granulomatous intestinal inflammation. Clinical paratuberculosis is associated with a T-helper 2 biased humoral immune response and eventual death because of inability of the host to contain the infection. Recent reports have suggested that gamma-delta (gammadelta) T cells play a significant role in granuloma development and/or maintenance during initial stages of infection and may influence the subsequent adaptive immune response. The objective of this study was to use an in vivo bovine model to evaluate gammadelta T cells during the early host immune response to mycobacterial infection. We used immunofluorescent staining, hyperspectral microscopy, and computerized assisted morphometry to evaluate staining and distribution of gammadelta T cells during development of organized and unorganized granulomas. Our data suggest that bovine gammadelta T cell subsets are differentially recruited to early infection sites, and may be instrumental during the initial antimycobacterial host immune response as well as for granuloma organization.
Plattner
Brandon L
BL
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Doyle
Robert T
RT
Hostetter
Jesse M
JM
D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D003936:Diagnosis, Computer-Assisted; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D006099:Granuloma / Q000382:microbiology* / Q000473:pathology*; D008297:Male; D008853:Microscopy / Q000379:methods; D009164:Mycobacterium Infections / Q000276:immunology* / Q000517:prevention & control; D016927:Mycobacterium avium subsp. paratuberculosis; D013194:Staining and Labeling; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage; D014611:Vaccination
D032581:Tuberculosis Vaccines