title: Down-regulation synaptic vesicle protein 2A in the anterior temporal neocortex of patients with intractable epilepsy.
abstract: Synaptic vesicle protein 2A (SV2A) involvement has been reported in the animal models of epilepsy. The aim of this study was to investigate the expression of SV2A in human intractable epilepsy (IE) brain tissue. Using immunohistochemistry, immunofluorescence, and Western blot, we detected SV2A expression in tissue samples from the anterior temporal neocortex of 33 patients who had been surgically treated for IE. We compared these tissues with nine histologically normal anterior temporal lobe samples from controls. SV2A immunoreactive staining was 0.1651+/-0.0564 in patient group and 0.2347+/-0.0187 in the control group (p<0.05) using immunohistochemistry, and this finding was consistently observed with Western blot analysis (0.1727 +/- 0.0471 versus 0.3976+/-0.0983, p<0.05). Immunofluorescence staining showed that SV2A was mainly accumulated in neurons. Our findings demonstrate that down-regulation of SV2A is present in patients with temporal lobe epilepsy.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Guibo
authors.initials: G
authors.lastname: Feng
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Zuchun
authors.initials: Z
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xi
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D015536:Down-Regulation / Q000502:physiology*; D004827:Epilepsy / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007120:Immunochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D019579:Neocortex / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D013702:Temporal Lobe / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D055815:Young Adult
chemical_list: D015415:Biomarkers; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C076414:SV2A protein, human