Autoantibody screen in inflammatory myopathies high prevalence of antibodies to gliadin.
BACKGROUND
Inflammatory myopathies (IM) are associated with autoimmune diseases.


OBJECTIVE
To evaluate the titers of auto-antibodies specific to various autoimmune diseases in patients with IM compared with controls.


METHODS
Sera from 99 IM patients and 100 healthy controls were tested for autoantibodies for vasculitis (myeloperoxidase, PR3, and glomerular basement membrane) and autoimmune gastrointestinal diseases (IgA and IgG antigliadin, antitissue transglutaminase, and Saccharomyces cerevisiae) utilizing the BioPlex 2200 Multiplexed Immunoassay method (Biorad).


RESULTS
Antigliadin IgA levels were significantly elevated in IM patients compared with controls (0.37 units +/- 0.44 vs. 0.24 units +/- 0.15, P = 0.017). Antitissue transglutaminase IgA was marginally increased in IM patients versus controls (0.36 units +/- 1.12 vs. 0.2 units +/- 0.0, P = 0.08).


CONCLUSIONS
Antibodies to gliadin and tissue transglutaminase characteristic for celiac disease were elevated in patients with IM compared with controls. This may indicate a higher prevalence of gluten sensitivity or celiac disease in IM.
Orbach
Hedi
H
Department of Medicine B, Wolfson Medical Center, Holon, Israel.
Amitai
Nimrod
N
Barzilai
Ori
O
Boaz
Mona
M
Ram
Maya
M
Zandman-Goddard
Gisele
G
Shoenfeld
Yehuda
Y
D001323:Autoantibodies / Q000097:blood*; D002446:Celiac Disease / Q000175:diagnosis; D003430:Cross-Sectional Studies; D005260:Female; D019204:GTP-Binding Proteins; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D009220:Myositis / Q000175:diagnosis*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D011503:Transglutaminases / Q000276:immunology; D014657:Vasculitis / Q000175:diagnosis
D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins