Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases.
We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).
Yan
Yajun
Y
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531, Boelter Hall, 420 Westwood Plaza, Los Angeles, CA 90095, USA.
Lee
Chia-Chi
CC
Liao
James C
JC
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002072:Butylene Glycols / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D013237:Stereoisomerism; D013379:Substrate Specificity
D002072:Butylene Glycols; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP)