Targeted cell adhesion on selectively micropatterned polymer arrays on a poly(dimethylsiloxane) surface.
Herein, we introduce the fabrication of polymer micropattern arrays on a chemically inert poly(dimethylsiloxane) (PDMS) surface and employ them for the selective adhesion of cells. To fabricate the micropattern arrays, a mercapto-ester-based photocurable adhesive was coated onto a mercaptosilane-coated PDMS surface and photopolymerized using a photomask to obtain patterned arrays at the microscale level. Robust polymer patterns, 380 microm in diameter, were successfully fabricated onto a PDMS surface, and cells were selectively targeted toward the patterned regions. Next, the performance of the cell adhesion was observed by anchoring cell adhesive linker, an RGD oligopeptide, on the surface of the mercapto-ester-based adhesive-cured layer. The successful anchoring of the RGD linker was confirmed through various surface characterizations such as water contact angle measurement, XPS analysis, FT-IR analysis, and AFM measurement. The micropatterning of a photocurable adhesive onto a PDMS surface can provide high structural rigidity, a highly-adhesive surface, and a physical pathway for selective cell adhesion, while the incorporated polymer micropattern arrays inside a PDMS microfluidic device can serve as a microfluidic platform for disease diagnoses and high-throughput drug screening.
Tang
Linzhi
L
Gachon BioNano Research Institute & Division of BioNano Technology and College of BioNano Technology, Kyungwon University, San 65, Bokjeong-dong, Sujeong-gu, Seongnam, Gyeonggi-do 461-701, South Korea.
Min
Junhong
J
Lee
Eun-Cheol
EC
Kim
Jong Sung
JS
Lee
Nae Yoon
NY
D001672:Biocompatible Materials / Q000737:chemistry*; D001681:Biological Assay / Q000295:instrumentation*; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D017076:Computer-Aided Design; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008422:Materials Testing; D046228:Microarray Analysis / Q000295:instrumentation*; D008904:Miniaturization; D013499:Surface Properties
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; C013830:baysilon