title: Efficient synthesis and evaluation of quorum-sensing modulators using small molecule macroarrays.
abstract: A method for the synthesis of small molecule macroarrays of N-acylated L-homoserine lactones (AHLs) is reported. A focused library of AHLs was constructed, and the macroarray platform was found to be compatible with both solution and agar-overlay assays using quorum-sensing (QS) reporter strains. Several QS antagonists were discovered and serve to showcase the macroarray as a straightforward technique for QS research.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706-1322, USA.
authors.forename: Thanit
authors.initials: T
authors.lastname: Praneenararat
authors.forename: Grant D
authors.initials: GD
authors.lastname: Geske
authors.forename: Helen E
authors.initials: HE
authors.lastname: Blackwell
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000215:Acylation; D048908:Aliivibrio fischeri / Q000166:cytology / Q000187:drug effects; D002861:Chromobacterium / Q000166:cytology / Q000187:drug effects; D003500:Cyclization; D009584:Nitrogen / Q000737:chemistry; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: C088386:homoserine lactone; D009584:Nitrogen; D015107:4-Butyrolactone