title: [Sensitivity of interferon-gamma release assay T-SPOT. TB in diagnosing extrapulmonary tuberculosis].
abstract: OBJECTIVE
  To evaluate the sensitivity of an interferon-gamma release assay T-SPOT. TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis.
  
  
  METHODS
  Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT. TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells (PBMCs).
  
  
  RESULTS
  T-SPOT. TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5% (95% CI 84.8% - 100%). The median of spot forming cells (SFCs) in response to early secreting antigen target 6 peptides was 196/10(6) PBMCs (interquartile range, 72-532/10(6) PBMCs), the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/10(6) PBMCs (interquartile range, 72-568/10(6) PBMCs), and the median of the incorporate SFCs was 612/10(6) PBMCs (interquartile range, 192-1 152/10(6) PBMCs).
  
  
  CONCLUSIONS
  T-SPOT. TB is highly sensitive in diagnosing extrapulmonary tuberculosis.
authors.affiliation: Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Huo
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Zhang
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Liu
mesh_terms: D006801:Humans; D007371:Interferon-gamma; D059425:Interferon-gamma Release Tests; D007963:Leukocytes, Mononuclear; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D007371:Interferon-gamma