title: Polyhydroxyalkanoates production by actinobacteria isolated from soil.
abstract: Polyhydroxyalkanoates (PHAs) are biodegradable and renewable polymers produced by a wide range of bacterial groups. New microbial bioprospection approaches have become an important way to find new PHA producers and new synthesized polymers. Over the past years, bacteria belonging to actinomycetes group have become known as PHA producers, such as Nocardia and Rhodococcus species, Kineosphaera limosa Liu et al. 2002, and, more recently, Streptomyces species. In this paper, we disclose that there are more actinobacteria PHA producers in addition to the genera cited. Some unusual genera, such as Streptoalloteichus, and some genera frequently present in soil, such as Streptacidiphilus, have been found. Thirty-four isolates were able to accumulate poly(3-hydroxybutyrate) and a number of these have traces of poly(3-hydroxyvalerate) when cultivated on glucose or glucose and casein as carbon source. Furthermore, some strains showed traces of medium chain length PHA. Transmission electron microscopy demonstrated that the PHA accumulation occurs in hyphae and spores.
authors.affiliation: Universidade de São Paulo, Instituto de Ciências Biomédicas II, Av. Professor Lineu Prestes, 1374 São Paulo, São Paulo 05508-900, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Matias
authors.forename: Diego
authors.initials: D
authors.lastname: Bonatto
authors.forename: Gabriel
authors.initials: G
authors.lastname: Padilla
authors.forename: Maria Filomena de Andrade
authors.initials: MF
authors.lastname: Rodrigues
authors.forename: João Antonio Pêgas
authors.initials: JA
authors.lastname: Henriques
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D012989:Soil Pollutants