[Genomic instability in sunflower interspecific hybrids].
The expression of genomic instability was studied at the phenotypical (morphological characters, electrophoretic spectra of seed storage proteins) and molecular (DNA amplification products) levels in interspecific hybrids (ISHs) from crosses of inbred lines of cultivated sunflower Helianthus annuus with perennial species of the genus Helianthus and in introgressive lines (ILs) produced on their basis. Unstable state of the locus determining the trait of lower branching was proved by the method of hybridological analysis. It was shown with the use of RAPD markers that the IL genome is characterized by instability even after long-term inbreeding (in generations F8-F12). In progenies of different combinations of interspecific crosses, identical polymorphous variants-were revealed for a seed storage protein, helianthinin, and for DNA fragments homologous to structural genes of functionally important proteins, suggesting the nonrandom character of ISH genome variation. This variation may be determined by genome reorganizations under the action of a genome shock induced by interspecific hybridization. The factors inducing reorganizations in the genome include the activity of mobile genetic elements (MGEs). Using primers specific to different MGE families, nucleotide sequences with a high level of homology to the sequences of fragments of the mobile elements MuDR, Far1, CACTA, Stowaway, and Tourist were identified in the sunflower genome. The possibility of using MGE fragments for sunflower genotyping was demonstrated.
Anisimova
I N
IN
Tumanova
L G
LG
Gavrilova
V A
VA
Diagileva
A V
AV
Pasha
L I
LI
Mitin
V A
VA
Timofeeva
G I
GI
D002678:Chimera / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D042822:Genomic Instability / Q000235:genetics*; D006368:Helianthus / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
D004251:DNA Transposable Elements; D010940:Plant Proteins