title: In vitro evaluation of the bioactive factors preserved in porcine small intestinal submucosa through cellular biological approaches.
abstract: The objective of this study was to develop cellular biological approaches to evaluate the potential effect of bioactive factors in porcine small intestinal submucosa (SIS) on bladder regeneration and angiogenesis. For this purpose, we cultured human bladder smooth muscle cell (HBSMC) and human umbilical vein endothelial cell (HUVEC), and then used cellular biological techniques to characterize in vitro biological effect of SIS components on HBSMC and HUVEC. Our results indicated that the SIS components had stimulated the attachment, proliferation, and migration of HBSMC and HUVEC, as well as tube formation by HUVEC on Matrigel. These results implied that the SIS might have preserved a mixture of bioactive factors including cell adhesion factors, mitogenic factors, chemotactic cytokines, and angiogenic factors, and these bioactive factors would have the potential of promoting bladder regeneration and angiogenesis. In conclusion, these cellular biological approaches might be helpful and effective for evaluation of the bioactive factors preserved in porcine SIS before it is used for bladder augmentation in humans.
authors.affiliation: Department of Urology, Affiliated Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210008, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Liuhua
authors.initials: L
authors.lastname: Zhou
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Yutian
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D014471:Umbilical Veins / Q000166:cytology; D001743:Urinary Bladder / Q000166:cytology
chemical_list: D001685:Biological Factors; D017077:Culture Media, Conditioned