The completion of the Mammalian Gene Collection (MGC).
Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.
Temple
Gary
G
Gerhard
Daniela S
DS
Rasooly
Rebekah
R
Feingold
Elise A
EA
Good
Peter J
PJ
Robinson
Cristen
C
Mandich
Allison
A
Derge
Jeffrey G
JG
Lewis
Jeanne
J
Shoaf
Debonny
D
Collins
Francis S
FS
Jang
Wonhee
W
Wagner
Lukas
L
Shenmen
Carolyn M
CM
Misquitta
Leonie
L
Schaefer
Carl F
CF
Buetow
Kenneth H
KH
Bonner
Tom I
TI
Yankie
Linda
L
Ward
Ming
M
Phan
Lon
L
Astashyn
Alex
A
Brown
Garth
G
Farrell
Catherine
C
Hart
Jennifer
J
Landrum
Melissa
M
Maidak
Bonnie L
BL
Murphy
Michael
M
Murphy
Terence
T
Rajput
Bhanu
B
Riddick
Lillian
L
Webb
David
D
Weber
Janet
J
Wu
Wendy
W
Pruitt
Kim D
KD
Maglott
Donna
D
Siepel
Adam
A
Brejova
Brona
B
Diekhans
Mark
M
Harte
Rachel
R
Baertsch
Robert
R
Kent
Jim
J
Haussler
David
D
Brent
Michael
M
Langton
Laura
L
Comstock
Charles L G
CL
Stevens
Michael
M
Wei
Chaochun
C
van Baren
Marijke J
MJ
Salehi-Ashtiani
Kourosh
K
Murray
Ryan R
RR
Ghamsari
Lila
L
Mello
Elizabeth
E
Lin
Chenwei
C
Pennacchio
Christa
C
Schreiber
Kirsten
K
Shapiro
Nicole
N
Marsh
Amber
A
Pardes
Elizabeth
E
Moore
Troy
T
Lebeau
Anita
A
Muratet
Mike
M
Simmons
Blake
B
Kloske
David
D
Sieja
Stephanie
S
Hudson
James
J
Sethupathy
Praveen
P
Brownstein
Michael
M
Bhat
Narayan
N
Lazar
Joseph
J
Jacob
Howard
H
Gruber
Chris E
CE
Smith
Mark R
MR
McPherson
John
J
Garcia
Angela M
AM
Gunaratne
Preethi H
PH
Wu
Jiaqian
J
Muzny
Donna
D
Gibbs
Richard A
RA
Young
Alice C
AC
Bouffard
Gerard G
GG
Blakesley
Robert W
RW
Mullikin
Jim
J
Green
Eric D
ED
Dickson
Mark C
MC
Rodriguez
Alex C
AC
Grimwood
Jane
J
Schmutz
Jeremy
J
Myers
Richard M
RM
Hirst
Martin
M
Zeng
Thomas
T
Tse
Kane
K
Moksa
Michelle
M
Deng
Merinda
M
Ma
Kevin
K
Mah
Diana
D
Pang
Johnson
J
Taylor
Greg
G
Chuah
Eric
E
Deng
Athena
A
Fichter
Keith
K
Go
Anne
A
Lee
Stephanie
S
Wang
Jing
J
Griffith
Malachi
M
Morin
Ryan
R
Moore
Richard A
RA
Mayo
Michael
M
Munro
Sarah
S
Wagner
Susan
S
Jones
Steven J M
SJ
Holt
Robert A
RA
Marra
Marco A
MA
Lu
Sun
S
Yang
Shuwei
S
Hartigan
James
J
Graf
Marcus
M
Wagner
Ralf
R
Letovksy
Stanley
S
Pulido
Jacqueline C
JC
Robison
Keith
K
Esposito
Dominic
D
Hartley
James
J
Wall
Vanessa E
VE
Hopkins
Ralph F
RF
Ohara
Osamu
O
Wiemann
Stefan
S
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D019295:Computational Biology / Q000379:methods*; D004247:DNA / Q000096:biosynthesis; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D005796:Genes / Q000235:genetics*; D006801:Humans; D008322:Mammals / Q000235:genetics*; D051379:Mice; D009316:National Institutes of Health (U.S.); D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014481:United States
D018076:DNA, Complementary; D004247:DNA