title: [Effects of c-Met-siRNA on the biological behaviour of laryngeal carcinoma Hep-2 cells].
abstract: OBJECTIVE
  To explore the effects of c-Met-siRNA on the proliferation, movement and invasion of laryngeal carcinoma Hep-2 cells in vitro.
  
  
  METHODS
  Firstly, the pSilencer 2.0/c-Met-shRNA recombinant plasmid was transfected into laryngeal carcinoma Hep-2 cells with transfecting agent of cationic liposome Lipofectamine 2000. Secondly,the transfection efficacy was tested by RT-PCR and Western-Blot, then the most inhibitive c-Met-siRNA sequence was elected. Cell proliferation, movement and invasion were detected with MTT, cell migration assay and cell invasion assay, respectively.
  
  
  RESULTS
  After the transfection of pSilencer 2.0/c-Met-shRNA recombinant plasmid into laryngeal carcinoma Hep-2 cells, the expression of mRNA and protein of c-Met decreased significantly in Hep-2 cells, and ability of the proliferation, movement and invasion of laryngeal carcinoma Hep-2 cells were also inhibited.
  
  
  CONCLUSIONS
  The results indicated that c-Met-siRNA can down-regulated the expression of c-Met and markedly inhibited laryngeal carcinoma Hep-2 cell proliferation, movement and invasion. It may have the potential as a therapeutic modality to treat human laryngeal carcinoma.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Daping Hospital, China.
authors.forename: Zhinian
authors.initials: Z
authors.lastname: Xie
authors.forename: Changyou
authors.initials: C
authors.lastname: Ji
authors.forename: Jichuan
authors.initials: J
authors.lastname: Chen
authors.forename: Yi'nan
authors.initials: Y
authors.lastname: Wang
authors.forename: Liqian
authors.initials: L
authors.lastname: Guan
authors.forename: Hongtao
authors.initials: H
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Junhui
authors.initials: J
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D008081:Liposomes; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D008081:Liposomes; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019859:Proto-Oncogene Proteins c-met