title: Systematic evaluation of a tissue-engineered bone for maxillary sinus augmentation in large animal canine model.
abstract: The objective of this study is to systematically evaluate the effects of a tissue-engineered bone complex for maxillary sinus augmentation in a canine model. Twelve sinus floor augmentation surgeries in 6 animals were performed bilaterally and randomly repaired with the following 3 groups of grafts: group A consisted of tissue-engineered osteoblasts/beta-TCP complex (n=4); group B consisted of beta-TCP alone (n=4); group C consisted of autogenous bone obtained from iliac crest as a positive control (n=4). All dogs had uneventful healings following the surgery. Sequential polychrome fluorescent labeling, maxillofacial CT, microhardness tests, as well as histological and histomorphometric analyses indicated that the tissue-engineered osteoblasts/beta-TCP complex dramatically promoted bone formation and mineralization and maximally maintained the height and volume of elevated maxillary sinus. By comparison, both control groups of beta-TCP or autologous iliac bone showed considerable resorption and replacement by fibrous or fatty tissue. We thus conclude that beta-TCP alone could barely maintain the height and volume of the elevated sinus floor, and that the transplantation of autogenous osteoblasts on beta-TCP could promote earlier bone formation and mineralization, maximally maintain height, volume and increase the compressive strength of augmented maxillary sinus. This tissue engineered bone complex might be a better alternative to autologous bone for the clinical edentulous maxillary sinus augmentation.
authors.affiliation: Oral Bioengineering Lab (Oral Tissue Engineering Lab), Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai, China.
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Wang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lunguo
authors.initials: L
authors.lastname: Xia
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Sun
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Dongxia
authors.initials: D
authors.lastname: Ye
authors.forename: Hasan
authors.initials: H
authors.lastname: Uludağ
authors.forename: Xinquan
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration / Q000502:physiology; D004285:Dogs; D007150:Immunohistochemistry; D008443:Maxillary Sinus / Q000601:surgery*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D014057:Tomography, X-Ray Computed
chemical_list: D001672:Biocompatible Materials; D015675:Osteocalcin; D000469:Alkaline Phosphatase