Two proteins from Saccharomyces cerevisiae: Pfy1 and Pkc1, play a dual role in activating actin polymerization and in increasing cell viability in the adaptive response to oxidative stress.
In this work, we show that the proteins Pkc1 and Pfy1 play a role in the repolarization of the actin cytoskeleton and in cell survival in response to oxidative stress. We have also developed an assay to determine the actin polymerization capacity of total protein extracts using fluorescence recovery after photobleaching techniques and actin purified from rabbit muscle. This assay allowed us to demonstrate that Pfy1 promotes actin polymerization under conditions of oxidative stress, while Pkc1 induces actin polymerization and cell survival under all the conditions tested. Our assay also points to a relationship between Pkc1 and Pfy1 in the actin cytoskeleton polymerization that is required to adapt to oxidative stress.
Pujol
Nuria
N
Department de Ciències Mèdiques Bàsiques-IRBLleida, Faculty of Medicine, University of Lleida, Lleida. Spain.
Bonet
Carmel
C
Vilella
Felip
F
Petkova
Mima I
MI
Mozo-Villarías
Angel
A
de la Torre-Ruiz
Maria Angeles
MA
D000199:Actins / Q000378:metabolism*; D005656:Fungal Proteins; D050296:Microbial Viability; D018384:Oxidative Stress; D051304:Profilins / Q000502:physiology*; D011493:Protein Kinase C / Q000502:physiology*; D055503:Protein Multimerization; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D013312:Stress, Physiological
D000199:Actins; D005656:Fungal Proteins; C547108:PFY1 protein, S cerevisiae; D051304:Profilins; D029701:Saccharomyces cerevisiae Proteins; C490702:PKC1 protein, S cerevisiae; D011493:Protein Kinase C