title: Preparation and characterization of folate-poly(ethylene glycol)-grafted-trimethylchitosan for intracellular transport of protein through folate receptor-mediated endocytosis.
abstract: To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocyanate conjugated bovine serum albumin (FITC-BSA) were prepared under suitable weight ratio of copolymer to FITC-BSA by ionic interaction between the positively charged copolymers and the negatively charged FITC-BSA. Intracellular uptake of FITC-BSA was specifically enhanced in SKOV3 cells (folate receptor over-expressing cell line) through folate receptor-mediated endocytosis compared with A549 cells (folate receptor deficient cell line). Folate-PEG-g-TMC shows promise for intracellular transport of negatively charged therapeutic proteins into folate receptor over-expressing tumor cells.
authors.affiliation: Sichuan University, Chengdu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Song
authors.forename: Michael
authors.initials: M
authors.lastname: Darby
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Liang
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cai
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Chen
authors.forename: Yueqi
authors.initials: Y
authors.lastname: Bi
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Yang
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Xu
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Li
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Sun
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
authors.forename: Shixiang
authors.initials: S
authors.lastname: Hou
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011108:Polymers; D011956:Receptors, Cell Surface; D011092:Polyethylene Glycols; D048271:Chitosan; D005492:Folic Acid