Three-dimensional combined photoacoustic and optical coherence microscopy for in vivo microcirculation studies.
Photoacoustic microscopy is predominantly sensitive to optical absorption, while optical coherence tomography relies on optical backscattering. Integrating their complementary contrasts can provide comprehensive information about biological tissue. We have developed a dual-modality microscope that combines the two for studying microcirculation. Three-dimensional imaging of microvasculature and its local environment has been demonstrated at micrometer-order resolution using endogenous contrast in vivo.
Li
Li
L
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, 1 Brookings Drive, Campus Box 1097, St. Louis, Missouri 63130, USA.
Maslov
Konstantin
K
Ku
Geng
G
Wang
Lihong V
LV
D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D004423:Ear / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D008833:Microcirculation / Q000502:physiology*; D041623:Tomography, Optical Coherence / Q000379:methods*