title: Immobilization of glucose oxidase on a poly(dimethylsiloxane) layer by using poly(L-lysine) as a polymer backbone.
abstract: Poly(dimethylsiloxane) (PDMS)-coated platinum electrodes modified with glucose oxidase (GOx) have been prepared from poly(L-lysine) (polymer backbone), glutaraldehyde (cross-linking agent) and poly(ethylene glycol) units. To fabricate a GOx layer by applying cross-linking chemistries, the PDMS layer was treated with oxygen plasma to replace silane groups with silanol groups. Optimization for the chemical fabrication of a GOx layer resulted in a simple preparation of sensors with a wide detectable range (0.1-6.0 mM) and without interference from hydrogen peroxide produced by a GOx reaction and the other in biological samples.
authors.affiliation: Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan. yasu@sci.u-hyogo.ac.jp
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yasukawa
authors.forename: Eiji
authors.initials: E
authors.lastname: Maekawa
authors.forename: Fumio
authors.initials: F
authors.lastname: Mizutani
mesh_terms: D000327:Adsorption; D001786:Blood Glucose / Q000032:analysis; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004986:Ether / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D004129:Dimethylpolysiloxanes; D004800:Enzymes, Immobilized; D004986:Ether; D011107:Polylysine; D011092:Polyethylene Glycols; D010984:Platinum; D005949:Glucose Oxidase; D005947:Glucose; D005976:Glutaral