Fabrication of protein-anchoring surface by modification of SiO2 with liposomal bilayer.
SiO(2) surface was successfully modified with phospholipid bilayer for biocompatibility by covering the planar surface with vesicular liposomes. By applying heat to rupture the vesicle, they were converted into a planar form. To effectively decorate the bilayer with biological molecules such as a protein, BAM (biological anchor for membrane) was used as a linker. It is a linear assembly consisting of oleyl chain, polyethylene glycol, and NHS (N-hydroxysuccinimide). After a target protein (BSA) was conjugated with BAM by NHS replacement, the conjugate was effectively inserted to the phospholipid bilayer through the lipophilic interaction between the oleyl chain and the lipid bilayer. The entire process was monitored and quantitatively analyzed by QCM (quartz crystal microbalance). BSA-BAM conjugate showed approximately 12-fold higher binding efficiency to the lipid bilayer than BSA alone. From this result, we conclude that SiO(2) surface could be modified to a lipid bilayer surface so as to anchor a protein by the action of BAM.
Cho
Hyung-Min
HM
Department of Chemical Engineering, Hanyang University, Ansan 426-791, Republic of Korea.
Cho
Do Youn
DY
Jeon
Jun Yeoung
JY
Hwang
Sang Youn
SY
Ahn
Il Shin
IS
Choo
Jaebum
J
Lee
E K
EK
D000818:Animals; D002417:Cattle; D003460:Crystallization; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010783:Photometry; D011791:Quartz; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012822:Silicon Dioxide / Q000378:metabolism*; D013499:Surface Properties; D013696:Temperature
D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D011791:Quartz; D012710:Serum Albumin, Bovine; D006046:Gold; D012822:Silicon Dioxide