Selection of reference genes for real-time polymerase chain reaction analysis in tissues from Bombus terrestris and Bombus lucorum of different ages.
Quantitative real-time polymerase chain reaction (PCR) is an accurate and sensitive technique for gene expression analysis. However, it requires data normalization using reference genes. Here we assessed the stability of eight reference genes in the labial gland and fat body of the bumblebees Bombus terrestris and Bombus lucorum of different ages. To date, no reference genes have been identified for these species. Our data show that arginine kinase (AK) and phospholipase A2 (PLA2) are the most stable genes in both tissues of B. terrestris. The most stable genes for the labial gland and fat body of B. lucorum were found to be elongation factor 1alpha (EEF1A) and PLA2.
Hornáková
Darina
D
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 166 10 Prague 6, Czech Republic.
Matousková
Petra
P
Kindl
Jirí
J
Valterová
Irena
I
Pichová
Iva
I
D000199:Actins / Q000235:genetics; D000818:Animals; D001122:Arginine Kinase / Q000235:genetics; D001516:Bees / Q000235:genetics*; D005088:Exocrine Glands / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D054467:Phospholipases A2 / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012269:Ribosomal Proteins / Q000235:genetics; D014404:Tubulin / Q000235:genetics
D000199:Actins; D020648:Peptide Elongation Factor 1; D012269:Ribosomal Proteins; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D001122:Arginine Kinase; D054467:Phospholipases A2