TGF-beta induces fibroblast activation protein expression; fibroblast activation protein expression increases the proliferation, adhesion, and migration of HO-8910PM [corrected].
Several studies recognize cancer-stromal fibroblasts' role in cancer-cell invasion and metastasis. Through paracrine signaling molecules, TGF-beta and IL-1beta, cancer cells activate stromal fibroblasts and induce the expression of fibroblast activation protein (FAP). FAP, in turn, affects the proliferation, invasion and migration of the cancer cells. We report that TGF-beta and IL-1beta are important factors in inducing differentiation of myofibroblasts and expression of functional markers, notably alpha-SMA. We discover that TGF-beta is the dominant factor in promoting FAPalpha protein expression. This study also examines FAP's function in vitro by assaying the proliferation, migration and invasion of ovarian cancer cell line HO-8910PM.
Chen
He
H
Department of Pathology, Harbin Medical University, Harbin, PR China. chenhe_2008@yahoo.com.cn
Yang
Wei-Wei
WW
Wen
Qiu-Ting
QT
Xu
Li
L
Chen
Ming
M
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D010450:Endopeptidases; D004789:Enzyme Activation; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D018093:Gelatinases / Q000096:biosynthesis* / Q000494:pharmacology; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology / Q000502:physiology; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000201:enzymology / Q000473:pathology*; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000502:physiology*; D000099167:Fibroblast Activation Protein Alpha
D010450:Endopeptidases; D018093:Gelatinases; D053583:Interleukin-1beta; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D016212:Transforming Growth Factor beta; D000099167:Fibroblast Activation Protein Alpha