Bioactive TGF-beta levels can be preserved in plasma samples collected into heparin but not EDTA.
Quantifying TGF-beta is important for many research areas since its effects often are dose-dependently bidirectional. The post-transcriptional control of TGF-beta bioavailability points out the need to determine TGF-beta at the protein level. Studies measuring TGF-beta in peripheral blood have to avoid contamination with platelet-derived TGF-beta. Techniques to obtain platelet-poor plasma have been suggested, however, the impact of different anti-coagulants on artificial TGF-beta contamination has not been studied in detail. Here, we compare TGF-beta levels in blood samples collected into heparin and EDTA tubes, stored for 0.5-18 h at various temperatures. We show that contamination with latent TGF-beta can only be prevented by collecting the sample on ice. Importantly, levels of bioactive TGF-beta in blood collected into heparin but not EDTA tubes remained stable up to 18 h, even when kept at RT. Further in vitro experiments indicate that heparin prevents the activation of latent TGF-beta into its bioactive form probably by virtue of accelerating the complex-formation between AT-III and thrombin. Where precise measurement of latent TGF-beta in blood samples is required, samples need to be collected on ice; bioactive TGF-beta can be detected reliably in samples collected into heparin tubes even when stored at RT.
Walther
Michael
M
Medical Research Council Laboratories, Fajara, PO Box 273, Banjul, Gambia. mwalther@mrc.gm
Jallow
Isatou K
IK
Jeffries
David
D
Walther
Brigitte
B
D000328:Adult; D001793:Blood Preservation / Q000379:methods*; D001800:Blood Specimen Collection / Q000592:standards; D015925:Cryopreservation; D004492:Edetic Acid / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013696:Temperature; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000097:blood* / Q000737:chemistry; D055815:Young Adult
D016212:Transforming Growth Factor beta; D006493:Heparin; D004492:Edetic Acid