title: Microfluidic device for single-molecule experiments with enhanced photostability.
abstract: A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Lemke
authors.forename: Yann
authors.initials: Y
authors.lastname: Gambin
authors.forename: Virginia
authors.initials: V
authors.lastname: Vandelinder
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brustad
authors.forename: Hsiao-Wei
authors.initials: HW
authors.lastname: Liu
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
authors.forename: Alex
authors.initials: A
authors.lastname: Groisman
authors.forename: Ashok A
authors.initials: AA
authors.lastname: Deniz
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D046210:Microfluidic Analytical Techniques; D038761:Photobleaching
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon