Intratracheal instillation of bone marrow-derived cell in an experimental model of silicosis.
The time course of lung mechanics, histology, and inflammatory and fibrogenic mediators are analysed after intratracheal instillation (IT) of bone marrow-derived cells (BMDC) in a model of silicosis. C57BL/6 mice were randomly divided into SIL (silica, 20mg IT) and control (CTRL) groups (saline IT). At day 15, mice received saline or BMDC (2 x 10(6)cells) IT. The biodistribution of technetium-99m BMDC was higher in lungs compared with other organs. At days 30 and 60, lung mechanics, the area of granulomatous nodules, and mRNA expression of IL-1beta and TGF-beta were higher in SIL than CTRL animals. BMDC minimized changes in lung mechanics, the area of granulomatous nodules, and total cell infiltration at day 30, but these effects were no longer observed at day 60. Conversely, BMDC avoided the expression of IL-1beta at days 30 and 60 and TGF-beta only at day 30. In conclusion, BMDC therapy improved lung mechanics and histology, but this beneficial effect was not maintained in the course of injury.
Lassance
Roberta M
RM
Laboratory of Cellular and Molecular Physiology, Brazil.
Prota
Luiz Felipe M
LF
Maron-Gutierrez
Tatiana
T
Garcia
Cristiane S N B
CS
Abreu
Soraia C
SC
Pássaro
Caroline P
CP
Xisto
Debora G
DG
Castiglione
Raquel C
RC
Carreira
Humberto
H
Ornellas
Debora Santos
DS
Santana
Maria Cristina E
MC
Souza
Sergio A L
SA
Gutfilen
Bianca
B
Fonseca
Lea Miriam B
LM
Rocco
Patricia R M
PR
Morales
Marcelo M
MM
D000704:Analysis of Variance; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015609:Organotechnetium Compounds; D012333:RNA, Messenger / Q000378:metabolism; D015656:Respiratory Mechanics / Q000502:physiology; D012829:Silicosis / Q000473:pathology* / Q000601:surgery*; D013997:Time Factors; D014132:Trachea / Q000601:surgery*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D014183:Transplantation, Heterologous; D014998:Y Chromosome
D053583:Interleukin-1beta; D015609:Organotechnetium Compounds; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta