title: Assessment of deoxynivalenol (DON) adsorbents and characterisation of their efficacy using complementary in vitro tests.
abstract: Deoxynivalenol (DON) is a prevalent and resistant mycotoxin found in cereals and related products. Adsorbents appear to provide an opportunity to decrease DON absorption in animals but, due to their specificity, it is very difficult to evaluate their actual efficacy. It is pointless to extrapolate results obtained with one mycotoxin to another and even to extrapolate results obtained in vitro in buffer to an in vivo situation. We carried out experiments to characterize the properties of potential DON adsorbents. Initial tests in buffer pH 7 allowed us to focus on six adsorbents: activated charcoal, cholestyramin, Saccharomyces cerevisiae mannans, algal beta-glycan, fungal beta-glycan and leguminous plant. The use of equilibrium sorption models suggested a non-saturated phenomenon and involved variable mechanisms according to the specific material. Subsequent tests with a Caco-2 cell model showed a high reduction in DON cytotoxicity on proliferative intestinal cells and DON absorption by differentiated intestinal cells when adsorbent was added (except for cholestyramin). Otherwise, values were not always in accordance with those obtained in buffer. Our work allowed us to identify five potential DON adsorbents and to propose a complementary in vitro test allowing improved determination of adsorbent properties.
authors.affiliation: Umr Inra-Dger-Isara-Lyon, Métabolisme et Toxicologie Comparée des Xénobiotiques, AGRAPOLE, 69364 Lyon cedex 7, France. cavret@isara.fr
authors.forename: S
authors.initials: S
authors.lastname: Cavret
authors.forename: N
authors.initials: N
authors.lastname: Laurent
authors.forename: B
authors.initials: B
authors.lastname: Videmann
authors.forename: M
authors.initials: M
authors.lastname: Mazallon
authors.forename: S
authors.initials: S
authors.lastname: Lecoeur
mesh_terms: D000327:Adsorption; D000821:Animal Feed / Q000032:analysis / Q000633:toxicity; D000818:Animals; D018938:Caco-2 Cells; D002606:Charcoal; D002792:Cholestyramine Resin; D002523:Edible Grain / Q000737:chemistry / Q000633:toxicity; D007887:Fabaceae; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008351:Mannans; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D014255:Trichothecenes / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D008351:Mannans; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D014255:Trichothecenes; D002792:Cholestyramine Resin; C071736:betaglycan; D002606:Charcoal; C007262:deoxynivalenol