title: delta-Aminolevulinic acid dehydratase of Haloarcula argentinensis isolated from Tuz Lake in Turkey.
abstract: The delta-aminolevulinic acid dehydratase (ALAD) enzyme of a novel record for Turkish microbial flora was studied. The isolate I-113 was obtained from Tuz Lake in Turkey and identified as Haloarcula argentinensis. The ALAD enzyme of the isolate was assayed in order to determine its requirements and to be used as biomarker for lead pollution in it's ambient. In enzymic studies, the effects of various metals (Cd, Co, Mg, Mn, Ni, Pb, and Zn), pH (3-11), temperatures (25-55 degrees C), and salinity (15-25%) conditions have been examined. The data obtained from the studies were analyzed statistically by using Kruskal-Wallis, Mann-Whitney, correlation, regression, variance analysis, and significance tests were performed by using SPSS 10.0 for Windows. Although its optimum pH was determined as 7, it was still active at pH 3-11. The optimal temperature for the enzyme was observed to be 30 degrees C. Mn and Pb inhibited its activity significantly (p < 0.05) while Zn increased it slightly. The ALAD enzyme in H. argentinensis could be used as a biomarker for Pb contamination.
authors.affiliation: Biology Department, Faculty of Science and Literature, Afyon Kocatepe University, ANS Campus, Gazligol Yolu, 03200, Afyonkarahisar, Turkey.
authors.forename: S Elif
authors.initials: SE
authors.lastname: Korcan
authors.forename: M Burçin
authors.initials: MB
authors.lastname: Mutlu
authors.forename: I Hakkı
authors.initials: IH
authors.lastname: Ciğerci
authors.forename: Kiymet
authors.initials: K
authors.lastname: Güven
authors.forename: Muhsin
authors.initials: M
authors.lastname: Konuk
authors.forename: H Mehtap
authors.initials: HM
authors.lastname: Kutlu
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D019613:Haloarcula / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D007854:Lead / Q000032:analysis / Q000633:toxicity; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D014421:Turkey; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D007854:Lead; D000623:Porphobilinogen Synthase