Nucleoside diphosphate kinase-1 regulates hyphal development via the transcriptional regulation of catalase in Neurospora crassa.
A nucleoside diphosphate kinase-1-disrupted (ndk-1(RIP-1)) mutant was observed to be defective in aerial hyphal and conidial development. In this study, two types of hyphae, fine and thick, were observed in wild-type (Wt) strains. However, only fine-type hyphae were observed in the ndk-1(RIP-1) mutants. The ndk-1(RIP-1) mutants were stimulated by oxidative stress and constitutively expressed an antioxidant enzyme catalase (CAT)-3. Furthermore the ndk-1(RIP-1) mutants could form thick hyphae by oxidative stress and a disruption of cat-3. These results suggest that the loss of thick hyphae in the ndk-1(RIP-1) mutants may be caused by the over-expression of cat-3.
Lee
Bumkyu
B
Biosafety Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, Rural Development Administration, 150 Sooin-ro, Seodun-dong, Gwonseon-gu, Suwon 441-707, Republic of Korea.
Yoshida
Yusuke
Y
Hasunuma
Kohji
K
D002374:Catalase / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism*; D018384:Oxidative Stress; D014158:Transcription, Genetic
D002374:Catalase; D009701:Nucleoside-Diphosphate Kinase