A novel convenient method for high bacteriophage titer assay.
The recent various applications of phages (bacteriophages) including phage therapy have brought about a revival of phage investigation. The phage titer assay is indispensable for phage experiments. However, the conventional standard method is a plaque counting method which requires a little skill with tedious repeating operation. Furthermore, it is not directly applicable to high phage titers. In this paper, we describe a novel convenient "cross streak and paper disk assay method" for high titer concentration without plaque counting.
Yoshida
Kazuo
K
Department of Nutritional Sciences, Yasuda Women's University, Hiroshima 731-0153, Japan. kazuyosi@hiroshima-u.ac.jp
Nasu
Yuki
Y
Shitami
Nozomi
N
Toyoda
Hitoko
H
Takemura
Hitomi
H
Oomori
Kiyomi
K
D017123:Bacteriophage T7 / Q000254:growth & development; D001435:Bacteriophages / Q000254:growth & development*; D014776:Virus Cultivation / Q000379:methods*