Topical administration of cyclosporin A in a solid lipid nanoparticle formulation.
Cyclosporin A (CsA)-loaded solid lipid nanoparticles (SLN) were developed for improved skin penetration. CsA-loaded SLN, prepared using a hot homogenization method, were nano-sized (about 73 nm) with a slightly negative surface charge (about -16 mV) and stable under physiological conditions regardless of CsA incorporation. In vitro permeation studies using murine skin mounted in the Franz-type vertical diffusion assembly revealed that the skin permeation efficiency of CsA-loaded SLN was 2-fold higher than that of CsA-oil mixture in viable skin. Furthermore, topically administered CsA-loaded SLN relieved symptoms of atopic dermatitis (AD) in an in vivo murine model of AD by decreasing the T helper (Th) 2 cell-related cytokines interleukin (IL)-4 and -5. These results suggest that SLN are effective drug carriers for topical delivery andthat CsA-loaded SLN can be therapeutically applied in allergy-related skin disorders.
Kim
Sung Tae
ST
Department of Biochemistry, Division of Brain Korea, College of Medicine, Korea University, Seoul, Korea. sungtae7@snu.ac.kr
Jang
Dong-Jin
DJ
Kim
Jong Hyo
JH
Park
Joo Yeon
JY
Lim
Ji Soo
JS
Lee
So Yeon
SY
Lee
Kyung-Mi
KM
Lim
Soo-Jeong
SJ
Kim
Chong-Kook
CK
D000287:Administration, Topical; D000818:Animals; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D016572:Cyclosporine / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D003876:Dermatitis, Atopic / Q000188:drug therapy; D004563:Electrochemistry; D005260:Female; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D008081:Liposomes; D051379:Mice; D008812:Mice, Hairless; D053758:Nanoparticles; D010316:Particle Size; D012869:Skin Absorption
D007166:Immunosuppressive Agents; D008081:Liposomes; D016572:Cyclosporine