title: Identification of potential serum markers for endometrial cancer using protein expression profiling.
abstract: OBJECTIVE
  Screening method of endometrial cancer (EC) has not been established yet. Our study was to explore serum biomarkers of EC patients using surface-enhanced laser desorption and ionization-time-of-flight mass spectrometry (SELDI-TOF MS).
  
  
  METHODS
  Serum samples from 65 EC patients and 40 controls were analyzed by SELDI-TOF MS (training set). Single- and multi-variant analyses were performed to compare protein profiles in serum of EC patients and healthy controls. Subsequently, blind test set including 40 EC patients and 40 controls were analyzed for validation.
  
  
  RESULTS
  A panel of four biomarker candidates were selected in training set analysis. These markers could also distinguish stage I patients from controls. Among them, two biomarkers were purified and identified as apolipoprotein A1 and a modified form of apolipoprotein C1. Screening for blind test set using dual-biomarker analysis yielded a sensitivity of 82% and a specificity of 86%.
  
  
  CONCLUSIONS
  Involvement of apolipoproteins with EC is first suggested in this study. In addition to possibility of screening method for EC, findings of these new biomarkers might be related with carcinogenesis or predisposition to EC.
authors.affiliation: Department of Obstetrics and Gynecology, National Defense Medical College, Tokorozawa, Saitama, 359-8513, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takano
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Asakawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Goto
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Kita
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kudoh
authors.forename: Junzo
authors.initials: J
authors.lastname: Kigawa
authors.forename: Noriaki
authors.initials: N
authors.lastname: Sakuragi
authors.forename: Masaru
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Toru
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yaegashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Seto
authors.forename: Mieko
authors.initials: M
authors.lastname: Shiwa
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism; D001774:Blood Chemical Analysis / Q000379:methods; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D016889:Endometrial Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D009367:Neoplasm Staging; D040081:Protein Array Analysis / Q000379:methods; D012372:ROC Curve; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor