title: Evaluation of in vitro activity of two topical products against three organisms isolated from canine referral patients with otitis externa and cutaneous pyoderma.
abstract: Canine otitis externa and cutaneous pyoderma are common problems that are often associated with Staphylococcus intermedius, Pseudomonas aeruginosa, and Malassezia pachydermatis. In vitro activity of two topical products against these organisms isolated from canine referral patients were evaluated. Organisms were grown and diluted to a concentration equivalent to 10(7) colony-forming units (CFU) per mL and exposed to either a 0 or 1/5 dilution of Hexadene Flush with Spherulites (Virbac Animal Health Inc, Fort Worth, TX) or a 1/5 or 1/25 dilution of ResiCHLOR Lotion with Spherulites (Virbac Animal Health Inc, Fort Worth, TX) at time intervals from 1 to 30 minutes. Results showed that all three organisms were killed within 1 minute of contact time at 0 and 1/5 dilution of the flush. The lotion diluted to 1/5 also killed all three organisms. At 1/25 dilution, this lotion killed S. intermedius and P. aeruginosa within 1 minute of contact time, whereas M. pachydermatis was killed after 1 minute. The findings suggest that the two topical products exhibit efficacy against these common skin pathogens in vitro and can be useful in their clinical management.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088, USA.
authors.forename: O
authors.initials: O
authors.lastname: Okwumabua
authors.forename: F
authors.initials: F
authors.lastname: Goodman
authors.forename: O
authors.initials: O
authors.lastname: Elfassy
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical; D002710:Chlorhexidine / Q000008:administration & dosage / Q000494:pharmacology*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D008290:Malassezia / Q000187:drug effects / Q000302:isolation & purification; D010032:Otitis Externa / Q000382:microbiology / Q000662:veterinary*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification; D011711:Pyoderma / Q000382:microbiology / Q000662:veterinary*; D034367:Streptococcus intermedius / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D002710:Chlorhexidine