Anti-inflammatory and immunomodulatory effects of Baccharis trimera aqueous extract on induced pleurisy in rats and lymphoproliferation in vitro.
Baccharis trimera is a widespread South American plant known as "carqueja". Medicinal teas prepared from the aerial parts of this plant are used in folk medicine in cases of liver diseases and inflammatory processes. We evaluated the effects of aqueous extract of B. trimera in the experimental inflammatory model of carrageenan-induced pleurisy in rat. The injection of carrageenan into the pleural cavity induces an influx of cells and fluid accumulation with a large number of polymorphonuclear leukocytes and increase of protein levels. The inflammation parameters were attenuated when B. trimera (400 and 800 mg/kg, i.p.) was administrated 30 min before the carrageenan. The immunomodulatory effects were evaluated in vitro on human peripheral blood mononuclear cells. The extract in concentration of 25, 50 and 100 mg/mL presented inhibited the T-lymphocytes proliferation stimulated by phytohemagglutinin, but these extract concentrations also presented cytotoxic effect. These results showed that the aqueous extract of B. trimera has anti-inflammatory effect.
Paul
Elisiane Lozza
EL
Laboratório de Pesquisa em Biofísica Celular e Inflamação, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil.
Lunardelli
Adroaldo
A
Caberlon
Eduardo
E
de Oliveira
Cristiane Bernardes
CB
Santos
Roberto Christ Vianna
RC
Biolchi
Vanderlei
V
Bastos
Carolina Maria Alves
CM
Moreira
Karla Barbosa
KB
Nunes
Fernanda Bordignon
FB
Gosmann
Grace
G
de Oliveira
Jarbas Rodrigues
JR
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D031175:Baccharis / Q000276:immunology; D002351:Carrageenan / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008213:Lymphocyte Activation / Q000276:immunology*; D008517:Phytotherapy / Q000379:methods; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D010998:Pleurisy / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014867:Water
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007155:Immunologic Factors; D010936:Plant Extracts; D014867:Water; D002351:Carrageenan