Isolation of a Methylobacillus sp. that degrades microcystin toxins associated with cyanobacteria.
Sludge from cyanobacteria-salvaged yard in Meiliang Bay, Lake Taihu in Wuxi, China was cultured and acclimated by inoculating microcystins (MCs) extract. Strain J10 was isolated by degrading the MC-RR and MC-LR and was identified as Methylobacillus sp. Further research showed that both MC-LR and MC-RR could be completely degraded at 17h after inoculation of J10, and the degradation probably was mediated by oxygen. Different enzymes, oxygen-dependent as well as oxygen-independent, with MC-degrading activity were found in the different fractions of J10 culture. However, the enzymes mainly responsible for MC degradation by J10 were oxygen-dependent and were probably bound to cell wall or outside the cytoplasmic membrane.
Hu
Liang Bin
LB
School of Food, Henan Institute of Science and Technology, Xinxiang 453003, China.
Yang
Jing Dong
JD
Zhou
Wei
W
Yin
Yu Fen
YF
Chen
Jian
J
Shi
Zhi Qi
ZQ
D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry* / Q000187:drug effects; D020631:Methylobacillus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D052998:Microcystins / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010802:Phylogeny; D013997:Time Factors
D052998:Microcystins; C078588:microcystin; D010100:Oxygen