Effects of the inclusion of Equex STM into Tris-based extender on the motility of dog spermatozoa incubated at 5 degrees C.
The aim of this study was to estimate the effects of Equex STM on sperm motion characteristics in chilled dog semen extended in Tris-based diluent. Thirty-two ejaculates were collected from 12 proven German shepherd stud dogs. The sperm-rich fractions were diluted in Tris-based extender with 1% (v/v) Equex STM (sample A) and in Tris-based extender with no addition of detergent (sample B). The extended semen was incubated for 240 h at 5 degrees C and the motility parameters were evaluated by CASA system at 24-h intervals. Addition of Equex STM to Tris-based extender led to an initial activation of motion activity of spermatozoa, followed by a rapid decrease, shortening the lifespan of spermatozoa incubated at 5 degrees C. Computer-assisted sperm analysis clearly showed that Equex STM-induced changes of sperm motion characteristics resemble the hyperactivation (HA) of spermatozoa associated with the capacitation process.
Nizański
W
W
Department and Clinic of Reproduction, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland. wojtek.nizanski@poczta.onet.eu
Klimowicz
M
M
Partyka
A
A
Savić
M
M
Dubiel
A
A
D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004285:Dogs; D008297:Male; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*
D003451:Cryoprotective Agents