Quaternary diffusion coefficients in a protein-polymer-salt-water system determined by rayleigh interferometry.
We have experimentally investigated multicomponent diffusion in a protein-polymer-salt-water quaternary system. Specifically, we have measured the nine multicomponent diffusion coefficients, D(ij), for the lysozyme-poly(ethylene glycol)-NaCl-water system at pH 4.5 and 25 degrees C using precision Rayleigh interferometry. Lysozyme is a model protein for protein-crystallization and enzymology studies. We find that the protein diffusion coefficient, D(11), decreases as polymer concentration increases at a given salt concentration. This behavior can be quantitatively related to the corresponding increase in fluid viscosity only at low polymer concentration. However, at high polymer concentration (250 g/L), protein diffusion is enhanced compared to the corresponding viscosity prediction. We also find that a protein concentration gradient induces salt diffusion from high to low protein concentration. This effect increases in the presence of poly(ethylene glycol). Finally, we have evaluated systematic errors associated with measurements of protein diffusion coefficients by dynamic light scattering. This work overall helps characterize protein diffusion in crowded environments and may provide guidance for further theoretical developments in the field of protein crystallization and protein diffusion in such crowded systems, such as the cytoplasm of living cells.
Annunziata
Onofrio
O
Department of Chemistry, Texas Christian University, Fort Worth, Texas 76129, USA. o.annunziata@tcu.edu
Vergara
Alessandro
A
Paduano
Luigi
L
Sartorio
Roberto
R
Miller
Donald G
DG
Albright
John G
JG
D000818:Animals; D004058:Diffusion; D007368:Interferometry; D009113:Muramidase / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*; D013816:Thermodynamics; D014783:Viscosity; D014867:Water / Q000737:chemistry*
D012492:Salts; D014867:Water; D011092:Polyethylene Glycols; D009113:Muramidase