title: Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi.
abstract: Glutaredoxins play an important role in cellular functionality. A putative dithiol glutaredoxin is encoded in the genome of Trypanosoma cruzi. We cloned the gene and obtained the recombinant protein, which behaved as a typical thioltransferase. Activity was variable and dependent on the nature of reducer or oxidant agent used, or both. Epimastigote extracts exhibited similar activity, suggesting the occurrence of the protein in the parasite. Results support a redox scenario in T. cruzi, with glutaredoxin being involved mainly in reduction of glutathione disulfide as well as in deglutathionylation of target proteins.
authors.affiliation: Instituto de Agrobiotecnología del Litoral, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral., Ciudad Universitaria-Paraje El Pozo, S3000ZAA Santa Fe, Argentina.
authors.forename: Vanina E
authors.initials: VE
authors.lastname: Marquez
authors.forename: Diego G
authors.initials: DG
authors.lastname: Arias
authors.forename: Claudia V
authors.initials: CV
authors.lastname: Piattoni
authors.forename: Carlos
authors.initials: C
authors.lastname: Robello
authors.forename: Alberto A
authors.initials: AA
authors.lastname: Iglesias
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Guerrero
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D054477:Glutaredoxins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics
chemical_list: D054477:Glutaredoxins; D011994:Recombinant Proteins