title: Comparison between pp65 antigenemia assay and quantitative real-time polymerase chain reaction for detection of active cytomegalovirus infection in routine diagnostics.
abstract: Real-time polymerase chain reaction (PCR) and pp65 antigenemia assay for the detection of active cytomegalovirus infection in immunocompromised patients experiencing neutropenia after bone marrow or kidney transplantation have been compared with a special focus on evaluability and embedment in daily routine diagnostics. Investigating 334 specimens from 97 patients, real-time PCR was shown to be the superior assay with regard to the parameters focused on.
authors.affiliation: Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany. rlugert@gwdg.de
authors.forename: Raimond
authors.initials: R
authors.lastname: Lugert
authors.forename: Utz
authors.initials: U
authors.lastname: Reichard
authors.forename: Frank T
authors.initials: FT
authors.lastname: Hufert
authors.forename: Uwe
authors.initials: U
authors.lastname: Gross
mesh_terms: D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D010750:Phosphoproteins / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014180:Transplantation; D014763:Viral Matrix Proteins / Q000097:blood*
chemical_list: D004279:DNA, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa