title: [Catalytical properties of Arthrobacter nicotianae cells, a producer of glucose isomerase, immobilized in xerogel of silicium dioxide].
abstract: Arthrobacter nicotinanae cells, producers of glucose isomerase, were immobilized in xerogel of silicium dioxide, and properties of the resulted heterogeneous biocatalysts were investigated in the process of isomerization of monosaccharide (glucose and fructose). The glucose isomerase activity of the resulted biocatalysts was shown to be 10 U/g, on average, taking into account the loss of the activity upon the immobilization, which amounted to 50% of the cell activity in suspension. The rate of the fructose isomerization increased linearly in the range of 55-80 degrees C with the temperature coefficient 1.3. The biocatalysts were stable in this range; they were rapidly inactivated, however, at increasing temperature. The half-inactivation time was six to seven h and five min or less at 80 degrees C and 85 degrees C, respectively. The half-inactivation time of heterogeneous biocatalysts was 50-90 h in the periodic process of isomerization of 2 M monosaccharides at 60 degrees C in the presence of the immobilized Arthrobacter nicotinanae cells.
authors.forename: L V
authors.initials: LV
authors.lastname: Perminova
authors.forename: G A
authors.initials: GA
authors.lastname: Kovalenko
authors.forename: N A
authors.initials: NA
authors.lastname: Rudina
authors.forename: L I
authors.initials: LI
authors.lastname: Sapunova
authors.forename: I O
authors.initials: IO
authors.lastname: Tamkovich
authors.forename: A G
authors.initials: AG
authors.lastname: Lobanok
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000096:biosynthesis / Q000737:chemistry*; D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry*; D018914:Cells, Immobilized / Q000201:enzymology; D005632:Fructose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006358:Hot Temperature; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D005632:Fructose; D012822:Silicon Dioxide; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose