Site-specific modification of RNA by functionality-transfer ODN probes.
Efficient methods for the modification of RNA molecules have been expected as innovative biological tools and therapeutic methods. In this study, the development of a general method for site-specific RNA modification by the functionality-transfer ODN probes has been investigated. Site-specific and cytosine-selective RNA modifications were achieved by the functionality-transfer reaction. It was shown that the base and site-selectivity were due to the close proximity of the reactants in the DNA-RNA duplexes.
Onizuka
Kazumitsu
K
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Taniguchi
Yosuke
Y
Nishioka
Takamasa
T
Sasaki
Shigeki
S
D003596:Cytosine / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D015345:Oligonucleotide Probes; D012313:RNA; D003596:Cytosine