Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization.
We have developed 2'-O-methyl-RNAs having phosphorylated cyclohexane at the 5' and/or 3'-terminal adenine. These 2'-O-methyl-RNAs formed less stable duplex with the longer target RNA than that with the short target RNA. We tried to improve the short-RNA selective hybridization property by the synthesis of 2'-O-methyl-RNAs having terminal guanine modifications.
Seio
Kohji
K
Department of Life Science, Graduate School of Bioscience and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. kseio@bio.titech.ac.jp
Miyazaki
Kazuya
K
Kurohagi
Sayako
S
Masaki
Yoshiaki
Y
Tsunoda
Hirosuke
H
Ohkubo
Akihiro
A
Sekine
Mitsuo
M
D003510:Cyclohexanes / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D022661:RNA, Untranslated / Q000737:chemistry*
D003510:Cyclohexanes; D009841:Oligonucleotides; D022661:RNA, Untranslated; C506365:Cyclohexane