Enhancement of angiogenic and vasculogenic potential of endothelial progenitor cells by haptoglobin.
Endothelial progenitor cells (EPCs) were transfected with the haptoglobin (Hp) gene to investigate the effect of Hp on cell function. Hp potentiated the gene expression of various pro-angiogenic factors in the EPCs. The Hp-modified EPCs also increased in vitro tube formation on Matrigel compared with control cells. In hindlimb ischaemia models, Hp-EPCs showed a greater ability for improving blood perfusion and recovery from ischaemic injury. These results indicate that Hp improves EPC function in neovasculogenesis, which suggests that ex vivo modification of EPCs with the Hp gene can be applied to the treatment of vascular damage.
Park
Seon-Joo
SJ
Department of Natural Sciences, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of Korea.
Baek
Sang Hong
SH
Oh
Mi-Kyung
MK
Choi
Seung Hyun
SH
Park
Eun Hye
EH
Kim
Nam-Hoon
NH
Shin
Jong-Chul
JC
Kim
In-Sook
IS
D000818:Animals; D002454:Cell Differentiation; D042783:Endothelial Cells / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology; D006242:Haptoglobins / Q000235:genetics / Q000502:physiology*; D006614:Hindlimb / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000503:physiopathology; D051379:Mice; D018919:Neovascularization, Physiologic / Q000235:genetics*; D013234:Stem Cells / Q000502:physiology*; D014162:Transfection
D006242:Haptoglobins