title: Fetuin-A promotes primary keratinocyte migration: independent of epidermal growth factor receptor signalling.
abstract: Previously, we reported that fetuin-A is a major component of ovine foetal skin and significantly enhances 'wound closure' in primary keratinocyte cultures. In this study, we found that in human newborn foreskin, a high level of fetuin-A protein is detected throughout the dermis. However, in adult skin a low level of fetuin-A is observed throughout the epidermal and dermal layers, except at regions surrounding hair follicles and at the epidermal-dermal junction where the level of fetuin-A is relatively high. Fetuin-A significantly induces actin-rich protrusions in human primary keratinocytes. Interestingly, blockade of epidermal growth factor (EGF) receptor signalling has a limited effect on fetuin-A promoted 'wound closure' on primary human keratinocytes, but significantly inhibits fetuin-A's effect on HaCaT cells. These results indicate that high levels of fetuin-A may partially contribute to less scar formation in newborn foreskin and that the effect of fetuin-A on primary keratinocyte migration is independent of EGF receptor signalling.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Wang
authors.forename: Betsy S
authors.initials: BS
authors.lastname: Hung
authors.forename: Margit
authors.initials: M
authors.lastname: Kempf
authors.forename: Pei-Yun
authors.initials: PY
authors.lastname: Liu
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Dalley
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Saunders
authors.forename: Roy M
authors.initials: RM
authors.lastname: Kimble
mesh_terms: D001798:Blood Proteins / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D016211:Transforming Growth Factor alpha; D060749:alpha-2-HS-Glycoprotein
chemical_list: C556354:AHSG protein, human; D001798:Blood Proteins; D016211:Transforming Growth Factor alpha; D060749:alpha-2-HS-Glycoprotein; D004815:Epidermal Growth Factor; D066246:ErbB Receptors