title: Light-independent metabolomics of endophytic Thielavia subthermophila provides insight into microbial hypericin biosynthesis.
abstract: The possible microbial mechanism of hypericin (1) and emodin (2) biosynthesis was studied in axenic submerged culture conditions in the endophytic fungus Thielavia subthermophila, isolated from Hypericum perforatum. The growth and secondary metabolite production of the endophyte remained independent of the illumination conditions. This production remained unaltered on spiking the medium with 3 or 5 mM 2, although the biomass accumulation was reduced. Neither emodin anthrone (3) nor protohypericin (4) could be detected at any stage of fermentation, irrespective of either spiking or illumination conditions. The endophytic metabolites exhibited photodynamic cytotoxicity against the human acute monocytic leukemia cell line (THP-1), at 92.7 vs 4.9%, and 91.1 vs 1.0% viability by resazurin and ATPlite assays, in light and in the dark, respectively. In trying to ascertain the presence/expression of the candidate hyp-1 gene in the endophyte, it was revealed that the hyp-1 gene was absent in T. subthermophila, indicating that the biosynthetic pathway in the endophytic fungus might be different and/or governed by a different molecular mechanism than the host plant or host cell suspension cultures. We have discussed the biosynthetic principles and evolutionary implications relating to endophytic T. subthermophila based on the results obtained.
authors.affiliation: Institut für Umweltforschung (INFU), Technische Universität Dortmund, Otto-Hahn-Strasse 6, 44221 Dortmund, Germany.
authors.forename: Souvik
authors.initials: S
authors.lastname: Kusari
authors.forename: Sebastian
authors.initials: S
authors.lastname: Zühlke
authors.forename: Ján
authors.initials: J
authors.lastname: Kosuth
authors.forename: Eva
authors.initials: E
authors.lastname: Cellárová
authors.forename: Michael
authors.initials: M
authors.lastname: Spiteller
mesh_terms: D000873:Anthracenes; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D004354:Drug Screening Assays, Antitumor; D004642:Emodin / Q000378:metabolism; D006801:Humans; D020902:Hypericum / Q000382:microbiology*; D008027:Light; D055432:Metabolomics; D015394:Molecular Structure; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D020037:Sordariales / Q000737:chemistry* / Q000235:genetics
chemical_list: D000873:Anthracenes; D000970:Antineoplastic Agents; D010569:Perylene; C004965:hypericin; D004642:Emodin