title: Quantitative miRNA expression analysis: comparing microarrays with next-generation sequencing.
abstract: Recently, next-generation sequencing has been introduced as a promising, new platform for assessing the copy number of transcripts, while the existing microarray technology is considered less reliable for absolute, quantitative expression measurements. Nonetheless, so far, results from the two technologies have only been compared based on biological data, leading to the conclusion that, although they are somewhat correlated, expression values differ significantly. Here, we use synthetic RNA samples, resembling human microRNA samples, to find that microarray expression measures actually correlate better with sample RNA content than expression measures obtained from sequencing data. In addition, microarrays appear highly sensitive and perform equivalently to next-generation sequencing in terms of reproducibility and relative ratio quantification.
authors.affiliation: Exiqon, DK-2950 Vedbaek, Denmark. haw@exiqon.com
authors.forename: Hanni
authors.initials: H
authors.lastname: Willenbrock
authors.forename: Jesper
authors.initials: J
authors.lastname: Salomon
authors.forename: Rolf
authors.initials: R
authors.lastname: Søkilde
authors.forename: Kim Bundvig
authors.initials: KB
authors.lastname: Barken
authors.forename: Thomas Nøhr
authors.initials: TN
authors.lastname: Hansen
authors.forename: Finn Cilius
authors.initials: FC
authors.lastname: Nielsen
authors.forename: Søren
authors.initials: S
authors.lastname: Møller
authors.forename: Thomas
authors.initials: T
authors.lastname: Litman
mesh_terms: D015870:Gene Expression; D035683:MicroRNAs / Q000032:analysis* / Q000138:chemical synthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D035683:MicroRNAs