Improving soil protein extraction for metaproteome analysis and glomalin-related soil protein detection.
Soil contains low amounts of protein but high amounts of interfering substances. Current extraction methods for soil protein cannot produce high-quality samples suitable for proteomic analysis. To resolve the problem, we devised a sequential extraction method, through sequentially extracting soil in citrate and SDS buffers, followed by phenol extraction. The method allows for obtaining applicable 1-D and 2-D protein profiles with various agricultural soils and detecting glomalin-related soil protein. The method may be a valuable tool for soil proteomics.
Chen
Shaoning
S
College of Life Science, Henan Agricultural University, Zhengzhou, PR China.
Rillig
Matthias C
MC
Wang
Wei
W
D006023:Glycoproteins / Q000302:isolation & purification*; D056186:Metagenomics / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012987:Soil / Q000032:analysis*
D006023:Glycoproteins; D020543:Proteome; D012987:Soil