[Impacts of the formula of Suoquanwan(SQW) on expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney of rat polyuria model of Yang-deficiency].
OBJECTIVE
To observe the impacts of the formula of Suoquanwan (SQW) on the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney of rat polyuria model of Yang-deficiency.


METHODS
The model rats were induced by adenine (250 mg/kg) for 4 weeks, then treated respectively with SQW or dDAVP. The expression of AQP-2 mRNA and AVPR-V2 mRNA in kidney of Yang-deficiency model by realtime fluorescence quantitative PCR method were investigated.


RESULTS
In model rats, the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney decreased, dDAVP and SQW high dose could increased the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney. The others had no influence on the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney.


CONCLUSIONS
SQW can increase the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney of rat polyuria model of Yang-deficiency.
Cao
Hong-Ying
HY
Guangzhou University of TCM, Guangzhou 510405, China. hycao@gzhtcm.edu.cn
Wu
Qing-He
QH
Huang
Ping
P
He
Jin-Yang
JY
D000818:Animals; D051399:Aquaporin 2 / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D011141:Polyuria / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000235:genetics / Q000378:metabolism*; D016711:Yang Deficiency / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism
D051399:Aquaporin 2; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D017483:Receptors, Vasopressin