title: Comparison of nucleic acid extraction methods for the detection of Mycoplasma pneumoniae.
abstract: Four nucleic acid extraction procedures (2 automated and 2 manual) were compared for their efficiency at isolating Mycoplasma pneumoniae DNA. Oropharyngeal swabs from healthy volunteers were spiked with varying amounts of M. pneumoniae, extracted, and tested using real-time polymerase chain reaction. Our data indicate that both automated extraction methods consistently outperform the manual procedures.
authors.affiliation: Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Thurman
authors.forename: Kelley C
authors.initials: KC
authors.lastname: Cowart
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Winchell
mesh_terms: D001331:Automation; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D006801:Humans; D009175:Mycoplasma Infections / Q000175:diagnosis*; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004269:DNA, Bacterial