title: [The effect of Nifedipine and mechanical strain on matrix metalloproteinase expression in human periodontal ligament fibroblast].
abstract: OBJECTIVE
  To investigate the effect of Nifedipine on matrix metalloproteinase (MMP)-10 and MMP-13 expression in human periodontal ligament fibroblast (HPDLF) induced by mechanical strain in vitro.
  
  
  METHODS
  HPDLF was divided into four groups at random by mechanical strain of elongation 0%, 8%, 12% and 16%. Each group was divided into four subgroups again by the concentration of Nifedipine 0, 10, 30, 50 microm. The cells were imposed to state mechanical strain 12 h after incubated with Nifedipine 1 h. And then immunohistochemical staining was used to investigate the expressions of MMP-10 and MMP-13 in intracytoplasm.
  
  
  RESULTS
  In 0% group, there was no significant difference of MMP-10 and MMP-13 expression in all subgroups (P>0.05). The expressions of MMP-10 and MMP-13 were high in group 8%, 12% and 16% without Nifedipine, and increasing significantly with elongation (P<0.001). The expressions of MMP-10 and MMP-13 decreased with dose increasing (P<0.001) in HPDLF after imposed to Nifidepine.
  
  
  CONCLUSIONS
  The expressions of MMP-10 and MMP-13 induced by mechanical strain were inhibited by Nifedipine, which suggested calcium ions channels participate in expressions of MMP-10 and MMP-13 induced by loading.
authors.affiliation: Dept. of Orthodontics, School of Stomatology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ni
authors.forename: Miao-miao
authors.initials: MM
authors.lastname: Zhang
authors.forename: Jia-yin
authors.initials: JY
authors.lastname: Dai
authors.forename: Miao
authors.initials: M
authors.lastname: Sun
mesh_terms: D005347:Fibroblasts; D006801:Humans; D020782:Matrix Metalloproteinases; D009543:Nifedipine; D010513:Periodontal Ligament; D012333:RNA, Messenger
chemical_list: D012333:RNA, Messenger; D020782:Matrix Metalloproteinases; D009543:Nifedipine