title: Evaluation of a new ELISA assay for detection of BP230 autoantibodies in bullous pemphigoid.
abstract: The diagnosis of bullous pemphigoid is based on clinical observations and on the presence of autoantibodies directed against proteins of the dermoepidermal junction. Human recombinant BP180 and BP230 peptides have been used to develop new quantitative enzyme immunoassays (EIA) for the detection of specific antibodies. This study evaluated the sensitivity and specificity of a new immunoassay for the detection of BP230 autoantibodies and clinical correlations. Serum samples were tested from patients with bullous pemphigoid, other skin diseases, and from healthy donors. Autoantibodies anti-BP230 and anti-BP180 were assayed using the EIA method. Diagnostic specificity for both tests was over 98%; diagnostic sensitivity was 90% and 60% for anti-BP180 and anti-BP230, respectively. IgG anti-BP180 titers exhibited a significant correlation with disease activity. No patient in remission was positive for anti-BP230. In conclusion, anti-BP180 and anti-BP230 assays are useful in the diagnosis of bullous pemphigoid and provide information on disease activity.
authors.affiliation: Clinical Pathology Laboratory, Polyclinic of Bari, Bari, Italy. mtampoia@libero.it
authors.forename: Marilina
authors.initials: M
authors.lastname: Tampoia
authors.forename: Valeria
authors.initials: V
authors.lastname: Lattanzi
authors.forename: Antonietta
authors.initials: A
authors.lastname: Zucano
authors.forename: Danilo
authors.initials: D
authors.lastname: Villalta
authors.forename: Raffaele
authors.initials: R
authors.lastname: Filotico
authors.forename: Antonietta
authors.initials: A
authors.lastname: Fontana
authors.forename: Gino Antonio
authors.initials: GA
authors.lastname: Vena
authors.forename: Francesca
authors.initials: F
authors.lastname: Di Serio
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology*; D003598:Cytoskeletal Proteins / Q000276:immunology*; D000071821:Dystonin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009419:Nerve Tissue Proteins / Q000276:immunology*; D024041:Non-Fibrillar Collagens / Q000276:immunology; D010391:Pemphigoid, Bullous / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012871:Skin Diseases / Q000175:diagnosis; D000097805:Collagen Type XVII
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human