title: Plasmacytoid dendritic cells capture and cross-present viral antigens from influenza-virus exposed cells.
abstract: Among the different subsets of dendritic cells (DC), plasmacytoid dendritic cells (PDC) play a unique role in secreting large amounts of type I interferons upon viral stimulation, but their efficiency as antigen-presenting cells has not been completely characterized. We show here, by flow cytometry, with human primary blood PDC and with a PDC cell line, that PDC display poor endocytic capacity for soluble or cellular antigens when compared to monocyte-derived myeloid DC. However, immature PDC efficiently take up cellular material from live influenza-exposed cells, subsequently mature and cross-present viral antigens very efficiently to specific CD8+ T cells. Therefore, during viral infection PDC not only secrete immunomodulatory cytokines, but also recognize infected cells and function as antigen cross-presenting cells to trigger the anti-viral immune response.
authors.affiliation: Université Joseph Fourier, Grenoble, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Lui
authors.forename: Olivier
authors.initials: O
authors.lastname: Manches
authors.forename: Juliette
authors.initials: J
authors.lastname: Angel
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Molens
authors.forename: Laurence
authors.initials: L
authors.lastname: Chaperot
authors.forename: Joël
authors.initials: J
authors.lastname: Plumas
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D000998:Antiviral Agents / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000821:virology*; D004705:Endocytosis; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D016898:Interferon-alpha / Q000378:metabolism; D008213:Lymphocyte Activation; D009975:Orthomyxoviridae / Q000378:metabolism*
chemical_list: D000956:Antigens, Viral; D000998:Antiviral Agents; D016207:Cytokines; D016898:Interferon-alpha