Isolation of a novel leptin receptor gene promoter preferentially functioning in neuronal cells.
Leptin exerts its metabolic effects by binding to the leptin receptor (Ob-R). In humans, the promoter of the Ob-R gene-related protein (Ob-RGRP) gene and the B219/Ob-R promoter have been speculated to spatially and temporarily regulate Ob-R gene transcription; however, the promoter function of the Ob-R gene has not been directly analyzed. Using 5' rapid amplification of the cDNA end, we isolated novel ob-r transcripts starting from the 3' portions of the B219/ob-r 5'-untranslated region in the adult mouse brain. The proximal promoter containing these start sites showed robust activity in neuron-derived, but not non-neuronal cell lines. The promoter activity was comparable with that of the ob-rgrp promoter in a neuronal cell, but significantly weaker in non-neuronal cells. Deletion analyses identifying two enhancer elements were located in this promoter. The identification of a novel ob-r gene promoter might provide a useful tool to study neuron specific expression and hormonal regulation of the ob-r gene.
Satoh
Tetsurou
T
Department of Medicine and Molecular Science, Gunma University, Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Japan. tsato@showa.gunma-u.ac.jp
Yoshino
Satoshi
S
Katano
Akiko
A
Ishizuka
Takahiro
T
Tomaru
Takuya
T
Shibusawa
Nobuyuki
N
Hashimoto
Koshi
K
Yamada
Masanobu
M
Mori
Masatomo
M
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D054411:Receptors, Leptin / Q000235:genetics*; D017384:Sequence Deletion
D054411:Receptors, Leptin; C515447:leptin receptor, mouse