title: [Comparative studies on the amount of ginsenosides between the crown galls and Chinese medicinal materials of Panax quinquefolium].
abstract: OBJECTIVE
  To provide theoretical basis for the production of ginsenosides by using modern biotechnologies, the comparative studies of the amount of ginsenosides between the crown galls and Chinese medicinal materials of Panax quinquefolium were carried out.
  
  
  METHODS
  Total ginsenosides were determined by spectrophotometry, and the contents of ginsenoside Rb1, Rb3, Rc, Re were determined by HPLC.
  
  
  RESULTS
  (1) The total ginsenosides in the crown galls of Panax quinquefolium (aftert 27 days' cultivation) was almost as much as that of Chinese medicinal material of Panax quinquefolium. (2) The contents of ginsenoside Rb1, Re in the crown galls were about half of that in Chinese medicinal materials of Panax quinquefolium, and ginsenoside Rc was about 80%. But the amount of ginsenoside Rb3 in the crown galls of Panax quinquefolium was much more than that in Chinese medicinal materials of Panax quinquefolium as almost 15 times higher.
  
  
  CONCLUSIONS
  The crown galls of Panax quinquefolium may be a new potential resource for large-scale production of ginsenosides.
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China. zjh4164@sina.com
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhu
authors.forename: Wen-Qiong
authors.initials: WQ
authors.lastname: Wang
authors.forename: Le
authors.initials: L
authors.lastname: Tang
authors.forename: Yi-Rei
authors.initials: YR
authors.lastname: Sun
authors.forename: Rong-Min
authors.initials: RM
authors.lastname: Yu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000096:biosynthesis* / Q000302:isolation & purification; D005894:Panax / Q000378:metabolism*; D010598:Pharmacognosy; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal / Q000378:metabolism*; D011786:Quality Control; D015203:Reproducibility of Results; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D036145:Ginsenosides