title: Effects of ovariectomy and resistance training on MMP-2 activity in skeletal muscle.
abstract: Matrix metallopeptidases (MMPs) are crucial to the maintenance of healthy tissue. The aim of this study was to investigate MMP-2 activity in gastrocnemius, soleus, tibialis anterior (TA), and extensor digitorium longus (EDL) muscle after resistance training in ovariectomized rats. Wistar adult female rats were grouped into 7 groups (n = 10 per group): sedentary (Sed-Intact); ovariectomized sedentary (Sed-Ovx); pseudo-ovariectomized sedentary (Sed-Pseudo); acute exercise (AcuteEx-Intact); ovariectomized acute exercise (AcuteEx-Ovx); strength trained (ChronicEx-Intact); and ovariectomized strength trained (ChronicEx-Ovx). A 12-week resistance training period, during which the animals climbed a 1.1-m vertical ladder with weights secured to their tails, was used. The sessions were performed once every 3 days, with 4-9 climbs and 8-12 dynamic movements per climb. The MMP-2 activity was analyzed by zymography. There was higher MMP-2 activity in soleus muscle in the ChronicEx-Intact and ChronicEx-Ovx groups, and lower MMP-2 activity in the AcuteEx-Ovx group, compared with the Sed-Intact group (p < or =0.05). The Sed-Ovx and ChronicEx-Ovx groups presented lower MMP-2 activity than the Sed-Intact group in TA. There was higher MMP-2 activity in AcuteEx-Intact and the AcuteEx-Ovx compared with the Sed-Intact and Sed-Ovx in TA, respectively (p < or = 0.05). In TA and EDL, training increased MMP-2 activity in the Sed-Intact group. No statistically significant alterations were observed for gastrocnemius muscle. Strength training increases MMP-2 activity in soleus, TA, and EDL muscle, which may be important for muscle remodeling. Ovariectomy downregulates MMP-2 in TA and EDL, which may compromise muscle function.
authors.affiliation: Department of Physiological Sciences, Federal University of Sao Carlos, Sao Carlos, Brazil. jonatop@gmail.com
authors.forename: Jonato
authors.initials: J
authors.lastname: Prestes
authors.forename: Rita
authors.initials: R
authors.lastname: de Cássia Marqueti
authors.forename: Gilberto Eiji
authors.initials: GE
authors.lastname: Shiguemoto
authors.forename: Richard Diego
authors.initials: RD
authors.lastname: Leite
authors.forename: Guilherme Borges
authors.initials: GB
authors.lastname: Pereira
authors.forename: Heloisa Sobreiro
authors.initials: HS
authors.lastname: Selistre-de-Araújo
authors.forename: Vilmar
authors.initials: V
authors.lastname: Baldissera
authors.forename: Sérgio Eduardo
authors.initials: SE
authors.lastname: de Andrade Perez
mesh_terms: D000818:Animals; D005260:Female; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009119:Muscle Contraction; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000201:enzymology*; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D055070:Resistance Training; D013997:Time Factors
chemical_list: D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat