title: [Repair of radius bone defect with the nano-hydroxyapatite/collagen/calcium alginate].
abstract: OBJECTIVE
  To explore the osteogenic potential of the nano-hydroxyapatite/collagen/calcium alginate composite implanted in animals.
  
  
  METHODS
  Eighteen 3-month-old New Zealand white rabbits were adopted to prepare 15 mm segmental defect model at the middle part of radius. Rabbit models were randomly divided into experimental group and blank control group. Nano-hydroxyapatite/collagen/calcium alginate was implanted into the defects of experimental group. Four, 8, and 12 weeks after operation, all specimens were examined by X-ray and histological methods.
  
  
  RESULTS
  All the 18 rabbit models entered the final analysis. X-ray showed that osteotylus was seen in the whole defect area in the experimental group 12 weeks after operation, during which osteogenesis was more obvious than in weeks 4 and 8 and the bridge grafting of defect area was obviously visible. In the blank control group, osteotylus was only observed at the two ends of the defects, and no osteogenesis was found in the central part of the defect area. Histological examination showed that new osteoid formation was seen in internal porous zone in the experimental group in weeks 4 and 8; in week 12, more woven bone-like tissues were visible and trabecular-like structure was formed.
  
  
  CONCLUSIONS
  The nano-hydroxyapatite/ collagen/calcium alginate has good osteogenic potential.
authors.affiliation: Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Lan
authors.forename: Chu-Song
authors.initials: CS
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Zhi-De
authors.initials: ZD
authors.lastname: Lü
authors.forename: Qi-Fei
authors.initials: QF
authors.lastname: Xu
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Feng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D010012:Osteogenesis; D011817:Rabbits; D011884:Radius / Q000033:anatomy & histology*; D023822:Tissue Engineering; D014945:Wound Healing
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; C509317:nano-hydroxyapatite-collagen; D020723:Glucuronic Acid; D003094:Collagen; D017886:Durapatite