Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara americana.
An hsc70 homologue gene (Rahsc70) of the diptera Rhynchosciara americana was isolated and characterized. We were able to determine the mRNA sequence from an EST of salivary gland cDNA library, and a Rahsc70 cDNA cassette was used as a probe to isolate the genomic region from a genomic library. The mRNA expression of this gene parallels the 2B puff expansion, suggesting its involvement in protein processing, since this larval period corresponds to a high synthetic activity period. During heat shock stress conditions, hsc70 expression decreased. In situ hybridization of polytene chromosomes showed that the Rahsc70 gene is located near the C3 DNA puff. The cellular localization of Hsc70 protein showed this protein in the cytoplasm and in the nucleus.
de Andrade
Alexandre
A
Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, São Paulo, SP, 05508-900, Brazil.
Siviero
Fabio
F
Rezende-Teixeira
Paula
P
Santelli
Roberto Vicente
RV
Machado-Santelli
Glaucia Maria
GM
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004175:Diptera / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response; D006801:Humans; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny
D050883:HSC70 Heat-Shock Proteins; D019476:Insect Proteins