Characterization of a new insect cell line (NTU-YB) derived from the common grass yellow butterfly, Eurema hecabe (Linnaeus) (Pieridae: Lepidoptera) and its susceptibility to microsporidia.
A new lepidopteran cell line, NTU-YB, was derived from pupal tissue of Eurema hecabe (Linnaeus) (Pieridae: Lepidoptera). The doubling time of YB cells in TNM-FH medium supplemented with 8% FBS at 28 degrees C was 26.87h. The chromosome numbers of YB cells varied widely from 21 to 196 with a mean of 86. Compared to other insect cell lines, the YB cells produced distinct esterase, malate dehydrogenase, and lactate dehydrogenase isozyme patterns. Identity of the internal transcribed spacer region-I (ITS-I) of YB cells to E. hecabe larvae was 96% and to Eurema blanda larvae (tissue isolated from head) was 81%. The YB cells were permissive to Nosema sp. isolated from E. blanda and the infected YB cells showed obvious cytopathic effects after 3weeks post inoculation. The highest level of spore production was at 4weeks post inoculation when cells were infected with the Nosema isolate, and spore production was 1.34+/-0.9x10(6)spore/ml. Ultrastructrual studies showed that YB cells can host in vitro propagation of the E. blanda Nosema isolate, and developing stages were observed in the host cell nuclei as observed in the natural host, E. blanda. The NTU-YB cell line is also susceptible to Nosema bombycis.
Chen
Yun-Ru
YR
Department of Entomology, College of Bioresources and Agriculture, National Taiwan University, Taipei 106, Taiwan, ROC.
Solter
Leellen F
LF
Chien
Tsz-Ying
TY
Jiang
Ming-Han
MH
Lin
Hsieh-Fang
HF
Fan
Huai-Sheng
HS
Lo
Chu-Fang
CF
Wang
Chung-Hsiung
CH
D000818:Animals; D002080:Butterflies / Q000166:cytology* / Q000382:microbiology; D018929:Cell Culture Techniques; D002455:Cell Division; D002460:Cell Line; D021901:DNA, Intergenic / Q000737:chemistry; D019476:Insect Proteins / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007621:Karyotyping; D016818:Nosema / Q000302:isolation & purification / Q000502:physiology; D011679:Pupa / Q000166:cytology / Q000382:microbiology
D021901:DNA, Intergenic; D019476:Insect Proteins; D007527:Isoenzymes