Time-dependent regulation of muscle caveolin activation and insulin signalling in response to high-fat diet.
We studied the effect of high-fat diet on the expression and activation of the three caveolins in rat skeletal muscle and their association with the insulin signalling cascade. Initial response was characterized by increased signalling through Cav-1 and Cav-3 phosphorylation, suggesting that both participate in an initial acute response to the calorie surplus. Afterwards, Cav-1 signalling was slightly reduced, whereas Cav-3 remained active. Late chronic phase signalling through both proteins was impaired inducing a prediabetic state. Summarizing, caveolins seem to mediate a time-dependent regulation of insulin cascade in response to high-fat diet in muscle.
Gómez-Ruiz
Ana
A
Department of Biochemistry and Molecular Biology, University of Navarra, Pamplona, Spain.
de Miguel
Carlos
C
Campión
Javier
J
Martínez
J Alfredo
JA
Milagro
Fermín I
FI
D000818:Animals; D001835:Body Weight; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D051243:Caveolin 2 / Q000235:genetics / Q000378:metabolism*; D051244:Caveolin 3 / Q000235:genetics / Q000378:metabolism*; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage*; D006943:Hyperglycemia / Q000097:blood; D006946:Hyperinsulinism / Q000097:blood; D007328:Insulin / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
C495537:Cav1 protein, rat; C495546:Cav2 protein, rat; C495549:Cav3 protein, rat; D051242:Caveolin 1; D051243:Caveolin 2; D051244:Caveolin 3; D004041:Dietary Fats; D007328:Insulin; D012333:RNA, Messenger; D011972:Receptor, Insulin