Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization.
The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.
Concepcion
Joy
J
ForteBio, Inc, CA 94025, USA. concepcionjoy@netscape.net
Witte
Krista
K
Wartchow
Charles
C
Choo
Sae
S
Yao
Danfeng
D
Persson
Henrik
H
Wei
Jing
J
Li
Pu
P
Heidecker
Bettina
B
Ma
Weilei
W
Varma
Ram
R
Zhao
Lian-She
LS
Perillat
Donald
D
Carricato
Greg
G
Recknor
Michael
M
Du
Kevin
K
Ho
Huddee
H
Ellis
Tim
T
Gamez
Juan
J
Howes
Michael
M
Phi-Wilson
Janette
J
Lockard
Scott
S
Zuk
Robert
R
Tan
Hong
H
D000906:Antibodies / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055808:Drug Discovery / Q000379:methods; D007368:Interferometry / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D011506:Proteins / Q000737:chemistry; D013194:Staining and Labeling; D013997:Time Factors
D000906:Antibodies; D008024:Ligands; D011506:Proteins