Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm.
Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8-oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage. In all samples examined, increased levels of 8-oxoguanine were present only in those spermatozoa with fragmented DNA, suggesting a link between both DNA damage types.
Santiso
Rebeca
R
INIBIC-Complejo Hospitalario Universitario A Coruña, Unidad de Genética, Coruña, Spain.
Tamayo
María
M
Gosálvez
Jaime
J
Meseguer
Marcos
M
Garrido
Nicolás
N
Fernández
José Luis
JL
D015415:Biomarkers / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D053938:DNA Fragmentation; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
D015415:Biomarkers; D002843:Chromatin; D011933:Reagent Kits, Diagnostic; C024829:8-hydroxyguanine; D006147:Guanine