[Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus].
The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder, Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63% of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.
Ni
Zheng
Z
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Wei
Wei
W
Liu
Guang-Qing
GQ
Chen
Liu
L
Yu
Bin
B
Yun
Tao
T
Hua
Jiong-Gang
JG
Li
Shuang-Mao
SM
D000818:Animals; D017209:Apoptosis; D017250:Caliciviridae Infections / Q000235:genetics / Q000503:physiopathology* / Q000662:veterinary* / Q000821:virology; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000821:virology; D053938:DNA Fragmentation; D017929:Hemorrhagic Disease Virus, Rabbit / Q000502:physiology*; D011817:Rabbits
D053148:Caspase 3