[Design of experimental approaches on the base of standard proteins for testing blood biopreparations and immunoperoxidase conjugates specific to human and animal immunoglobulines G].
Using standard forms of immunoglobulin (Ig) G and albumin, we have studied electrophoretic and chromatographic profiles of samples of pharmaceutical blood biopreparation batches. The usability of standard proteins was also demonstrated by testing analytical characteristics of immunoperoxidase conjugates specific to human and animal IgG (anti-IgG IPC). In particular, we suggest an additional estimation of analytical characteristics of anti-IgG IPC by the enzyme reaction kinetics with the standard dilution which is calculated by the direct enzyme-liked immunoassay on the homologous IgG-antigen.
Barsukov
A K
AK
Barmin
A V
AV
Kuznetsov
A I
AI
Nesterova
O Iu
OIu
Ushnurtseva
S A
SA
Panin
A N
AN
Smolenskiĭ
V I
VI
Ulasov
V I
VI
Sviderskiĭ
V L
VL
Khovanskikh
A E
AE
D000818:Animals; D000918:Antibody Specificity; D001769:Blood; D001774:Blood Chemical Analysis / Q000379:methods / Q000592:standards; D006735:Horseradish Peroxidase; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D007124:Immunoenzyme Techniques / Q000379:methods / Q000592:standards*; D007074:Immunoglobulin G / Q000032:analysis*; D012015:Reference Standards
D018796:Immunoconjugates; D007074:Immunoglobulin G; D006735:Horseradish Peroxidase