[The inhibitory effect of calcitriol on the proliferation of hPDLCs populations of different vitamin D receptor Fok I genotypes].
OBJECTIVE
The study was designed to investigate the effect of calcitriol [1,25-(OH)(2)D(3),VD(3)] on the proliferation of human periodontal ligament cells (hPDLCs) and to analyze the influence of vitamin D receptor Fok I polymorphism (rs2228570, T/C) on the effect.


METHODS
Primary cultures of hPDLCs populations from individual donors were established. Restriction fragment length polymorphism method was used to detect the genotypes of the Fok I site. The fourth passage hPDLCs populations of FF, Ff and ff genotypes were respectively treated with 0.1% ethyl alcohol (control) or 10(-14)-10(-8) mol/L VD3 for 1, 3, 5 and 7 days. MTT method was performed to evaluate the proliferation of hPDLCs. The data was analyzed by one-way ANOVA and LSD Post Hoc test using SPSS 11.5 software package.


RESULTS
VD3 inhibited the proliferation of hPDLCs populations of three genotypes in a time- and concentration-dependent manner. 10(-8) mol/L VD(3) exerted the most powerful inhibition compared with controls and other concentrations and the highest inhibition occurred on day 5 or day 7. Compared with the corresponding controls, the lowest concentrations of VD3 which exerted statistically significant inhibitory effect on the proliferation of hPDLCs populations were listed as follows: 10(-12) mol/L for the hPDLCs population of FF genotype (P<0.01), 10(-10) for Ff genotype (P<0.01) and 10(-8) mol/L for ff genotype (P<0.01). The highest proliferation inhibitory rate exerted by 10(-8) mol/L VD(3) on the ff population was 16.93%,which was significantly lower than FF (31.20%, P<0.05) and Ff (29.68%, P<0.05). On day 3 and day 5, 10(-8) mol/L VD(3) exhibited the strongest suppression on the proliferation of hPDLCs population of FF genotype, followed by Ff and ff genotypes (P<0.01).


CONCLUSIONS
VD(3) can significantly inhibit the proliferation of hPDLCs, which may be affected by VDR Fok I polymorphism.
Tang
Xiao-lin
XL
Department of Periodontology, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China. xltang@mail.cmu.edu.cn
Meng
Huan-xin
HX
D002117:Calcitriol; D005838:Genotype; D006801:Humans; D010513:Periodontal Ligament; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol
D018167:Receptors, Calcitriol; D002117:Calcitriol