Chryseobacterium arthrosphaerae sp. nov., isolated from the faeces of the pill millipede Arthrosphaera magna Attems.
A bacterial strain (CC-VM-7T), isolated from the faeces of the pill millipede Arthrosphaera magna Attems collected in India, was studied to determine its taxonomic allocation. Cells stained Gram-negative and were rod-shaped. Comparative analyses of the 16S rRNA gene sequence of the strain with those of the most closely related species clearly suggested allocation to the genus Chryseobacterium, with the highest sequence similarities of 99.2% to Chryseobacterium gleum CCUG 14555T, 98.6% to Chryseobacterium indologenes CCUG 14556T and 98.4% to Chryseobacterium aquifrigidense KCTC 12894T. 16S rRNA gene sequence similarities to all other species of the genus Chryseobacterium were below 98%. The major whole-cell fatty acids were iso-C15:0 and iso-C17:1omega9c. DNA-DNA hybridization resulted in relatedness values of only 29.6% (reciprocal 31.3%) to Chryseobacterium gleum CCUG 14555T, 41.2% (reciprocal 38.8%) to C. indologenes CCUG 14556T and 35.4% (reciprocal 38.5%) to C. aquifrigidense KCTC 12894T. DNA-DNA relatedness, biochemical and chemotaxonomic properties clearly show that strain CC-VM-7T represents a novel species, for which the name Chryseobacterium arthrosphaerae sp. nov. is proposed. The type strain is CC-VM-7T (=CCUG 57618T =CCM 7645T).
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Arun
A B
AB
Yenepoya Research Center, Yenepoya University, University Road, Deralakatte, Mangalore 575 018, Karnataka, India.
Young
Chiu-Chung
CC
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Sridhar
K R
KR
Microbiology and Biotechnology, Department of Biosciences, Mangalore University, Mangalagangotri, Mangalore 574 199, Karnataka, India.
Rekha
P D
PD
Yenepoya Research Center, Yenepoya University, University Road, Deralakatte, Mangalore 575 018, Karnataka, India.
D000818:Animals; D001181:Arthropods / Q000382:microbiology*; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S