Bioavailability of catechins from ready-to-drink tea.
OBJECTIVE
Because consumption of teas may be associated with potential health benefits due to its content in polyphenols and in Western countries the consumption of tea is equally divided between the hot and the ready-to-drink (RTD) cold versions of this typical beverage, the aim of this work was to study the absorption and metabolism of flavan-3-ols in human volunteers after the ingestion of a commercial RTD tea.


METHODS
A feeding study was carried out in 20 healthy human volunteers and urine samples were collected for 24h after tea ingestion. Flavan-3-ols-derived molecules were identified and quantified in urine samples by high-performance liquid chromatography with tandem mass spectrometric detection.


RESULTS
Eight relevant metabolites were identified in urine, all modified flavan-3-ols with the exception of unmetabolized gallic acid. The urinary excretion of flavan-3-ols was equal to 7.2% of the intake with tea. Gallic acid, which was abundant in the RTD tea used in this study, reached a 4.5% of the drunken amount.


CONCLUSIONS
The bioavailability values observed are in agreement with previous reports, although the dosage of polyphenols ingested in this study is remarkably lower. Moreover, the use of a group of 20 volunteers, more than the average number of subjects used for usual human acute-feeding studies involving polyphenols, provides additional credibility to the results. After drinking the RTD ice tea used in this study, the internal compartments are exposed to non-marginal doses of flavanols and flavanol metabolites up to 24h.
Del Rio
Daniele
D
Department of Public Health, University of Parma, Parma, Italy.
Calani
Luca
L
Scazzina
Francesca
F
Jechiu
Lucia
L
Cordero
Chiara
C
Brighenti
Furio
F
D000293:Adolescent; D000328:Adult; D001682:Biological Availability; D002392:Catechin / Q000031:analogs & derivatives / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D005707:Gallic Acid / Q000008:administration & dosage / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry; D013662:Tea / Q000737:chemistry*
D005419:Flavonoids; D013662:Tea; C404987:flavan-3-ol; D005707:Gallic Acid; D002392:Catechin; C045651:epigallocatechin gallate; C057580:gallocatechol