Immortalized human brain endothelial cell line HCMEC/D3 as a model of the blood-brain barrier facilitates in vitro studies of central nervous system infection by Cryptococcus neoformans.
Cryptococcus neoformans cells must cross the blood-brain barrier prior to invading the central nervous system. Here we demonstrate that the immortalized human brain endothelial cell line HCMEC/D3 is a useful alternative to primary brain endothelial cells as a model of the blood-brain barrier for studies of central nervous system infection.
Vu
Kiem
K
Department of Pharmacology, School of Medicine, University of California, Genome and Biomedical Sciences Facility, Davis, CA, USA.
Weksler
Babette
B
Romero
Ignacio
I
Couraud
Pierre-Olivier
PO
Gelli
Angie
A
D001812:Blood-Brain Barrier / Q000378:metabolism / Q000382:microbiology*; D001921:Brain / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D002460:Cell Line; D002494:Central Nervous System Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D003453:Cryptococcosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D003455:Cryptococcus neoformans / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology* / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D008954:Models, Biological