Simultaneous saccharification and fermentation and partial saccharification and co-fermentation of lignocellulosic biomass for ethanol production.
Ethanol production by fermentation of lignocellulosic biomass-derived sugars involves a fairly ancient art and an ever-evolving science. Production of ethanol from lignocellulosic biomass is not avant-garde, and wood ethanol plants have been in existence since at least 1915. Most current ethanol production relies on starch- and sugar-based crops as the substrate; however, limitations of these materials and competing value for human and animal feeds is renewing interest in lignocellulose conversion. Herein, we describe methods for both simultaneous saccharification and fermentation (SSF) and a similar but separate process for partial saccharification and cofermentation (PSCF) of lignocellulosic biomass for ethanol production using yeasts or pentose-fermenting engineered bacteria. These methods are applicable for small-scale preliminary evaluations of ethanol production from a variety of biomass sources.
Doran-Peterson
Joy
J
Microbiology Department, University of Georgia, Athens, GA, USA.
Jangid
Amruta
A
Brandon
Sarah K
SK
DeCrescenzo-Henriksen
Emily
E
Dien
Bruce
B
Ingram
Lonnie O
LO
D000143:Acids / Q000494:pharmacology; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D002849:Chromatography, Gas / Q000379:methods; D000431:Ethanol / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D008954:Models, Biological; D011134:Polysaccharides / Q000378:metabolism*
D000143:Acids; D011134:Polysaccharides; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin