Imaging of the cytoskeleton and mitochondria in fixed budding yeast cells.
The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently, high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model eukaryote. However, it is now feasible for laboratories to acquire and analyze high-resolution multidimensional images of yeast cell biology. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing the cytoskeleton and interactions of the actin cytoskeleton with mitochondria in fixed yeast cells using fluorescence microscopy.
Swayne
Theresa C
TC
Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY, USA.
Boldogh
Istvan R
IR
Pon
Liza A
LA
D000199:Actins / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010590:Phalloidine / Q000378:metabolism; D012235:Rhodamines / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D015415:Biomarkers; D005456:Fluorescent Dyes; D012235:Rhodamines; D010590:Phalloidine