Biopharmaceutical production: Applications of surface plasmon resonance biosensors.
Surface plasmon resonance (SPR) permits the quantitative analysis of therapeutic antibody concentrations and impurities including bacteria, Protein A, Protein G and small molecule ligands leached from chromatography media. The use of surface plasmon resonance has gained popularity within the biopharmaceutical industry due to the automated, label free, real time interaction that may be exploited when using this method. The application areas to assess protein interactions and develop analytical methods for biopharmaceutical downstream process development, quality control, and in-process monitoring are reviewed.
Thillaivinayagalingam
Pranavan
P
Pfizer Biotechnology Ireland, Shanbally, Ringaskiddy, Cork, Ireland.
Gommeaux
Julien
J
McLoughlin
Michael
M
Collins
David
D
Newcombe
Anthony R
AR
D000906:Antibodies / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D055767:Immobilized Proteins / Q000737:chemistry*; D007163:Immunosorbent Techniques; D007700:Kinetics; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000379:methods*
D000906:Antibodies; D055767:Immobilized Proteins