title: Production of pure beta-glucan by Aureobasidium pullulans after pullulan synthetase gene disruption.
abstract: By disruption of the pullulan synthetase gene (pul) of Aureobasidium pullulans IMS822 KCTC11179BP, we constructed a mutant strain, A. pullulans NP1221, which produced a pure beta-glucan exopolysaccharide. The mutant NP1221 was white, whereas the wild-type strain produced a black dye. When we compared fermentation kinetics between wide-type and mutant strains, the mutant NP1221 did not produce pullulan. Substrate uptake rate and beta-glucan production were similar in both strains. However, the biomass yield of mutant NP1221 was 2.3-fold (9.2 g l(-1)) greater than that of wild-type.
authors.affiliation: Institute Bioindustry Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 1404, Sinjeong-dong, Jeungeup-si, Jeonbuk, 580-185, Korea.
authors.forename: Byung-Kwan
authors.initials: BK
authors.lastname: Kang
authors.forename: Hee-Jong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Nack-Shick
authors.initials: NS
authors.lastname: Choi
authors.forename: Keug-Hyun
authors.initials: KH
authors.lastname: Ahn
authors.forename: Chan-Sun
authors.initials: CS
authors.lastname: Park
authors.forename: Byung-Dae
authors.initials: BD
authors.lastname: Yoon
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Kim
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005936:Glucans / Q000235:genetics*; D007700:Kinetics; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D047071:beta-Glucans; C009109:pullulan