SSeCKS is a suppressor in Schwann cell differentiation and myelination.
Src-suppressed protein kinase C substrate (SSeCKS) plays an important role in the differentiation process. In regeneration of sciatic nerve injury, expression of SSeCKS decreases, mainly in Schwann cells. However, the function of SSeCKS in Schwann cells differentiation remains unclear. We observed that SSeCKS was decreased in differentiated Schwann cells. In long-term SSeCKS-reduced Schwann cells, cell morphology changed and myelin gene expression induced by cAMP was accelerated. Myelination was also enhanced in SSeCKS-suppressed Schwann cells co-culture with dorsal root ganglion (DRG). In addition, we found suppression of SSeCKS expression promoted Akt serine 473 phosphorylation in cAMP-treated Schwann cells. In summary, our data indicated that SSeCKS was a negative regulator of myelinating glia differentiation.
Ji
Yuhong
Y
Laboratory Center, Affiliated Hospital of Nantong University, and The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, 226001 Nantong, People's Republic of China.
Tao
Tao
T
Cheng
Chun
C
Yang
Huiguang
H
Wang
Youhua
Y
Yang
Junling
J
Liu
Haiou
H
He
Xinxing
X
Wang
Huiming
H
Shen
Aiguo
A
D054758:A Kinase Anchor Proteins / Q000502:physiology*; D000818:Animals; D018797:Cell Cycle Proteins / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects*; D018920:Coculture Techniques; D000242:Cyclic AMP / Q000502:physiology; D015536:Down-Regulation; D005727:Ganglia, Spinal / Q000502:physiology; D009186:Myelin Sheath / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology
D054758:A Kinase Anchor Proteins; C102361:Akap12 protein, rat; D018797:Cell Cycle Proteins; D000242:Cyclic AMP; D051057:Proto-Oncogene Proteins c-akt