Microfluidic device for single-molecule experiments with enhanced photostability.
A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.
Lemke
Edward A
EA
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Gambin
Yann
Y
Vandelinder
Virginia
V
Brustad
Eric M
EM
Liu
Hsiao-Wei
HW
Schultz
Peter G
PG
Groisman
Alex
A
Deniz
Ashok A
AA
D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D046210:Microfluidic Analytical Techniques; D038761:Photobleaching
D004129:Dimethylpolysiloxanes; C013830:baysilon