Live-cell imaging of the cytoskeleton and mitochondrial-cytoskeletal interactions in budding yeast.
This chapter describes labeling methods and optical approaches for live-cell imaging of the cytoskeleton and of a specific organelle-cytoskeleton interaction in budding yeast.
Swayne
Theresa C
TC
Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY, USA.
Lipkin
Thomas G
TG
Pon
Liza A
LA
D000199:Actins / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D008870:Microtubules / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D011726:Pyridinium Compounds / Q000378:metabolism; D020112:Rhodamine 123 / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods; D014335:Tropomyosin / Q000378:metabolism
D000199:Actins; D002232:Carbocyanines; D004396:Coloring Agents; D005456:Fluorescent Dyes; D007211:Indoles; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D011726:Pyridinium Compounds; D014335:Tropomyosin; C026742:plastin; D049452:Green Fluorescent Proteins; D020112:Rhodamine 123; C011786:2-(4-(dimethylamino)styryl)-1-methylpyridinium; C007293:DAPI; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine