Multipyrene tandem probes for detection of C677T polymorphism in MTHFR gene.
We designed tandems of oligo(2'-O-methylribonucleotides) conjugates containing two bispyrene (5'-bisPyr and 3'-bisPyr) groups on their junction for detection of C677T polymorphism in the methylenetetrahydrofolate reductase gene (MHTFR). The potential of SNP detection with multipyrene tandems of oligo(2'-O-methylribonucleotides) was demonstrated.
Kholodar
Svetlana A
SA
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk 630090, Russia. danov@niboch.nsc.ru
Novopashina
Daria S
DS
Meschaninova
Mariya I
MI
Lomzov
Alexander A
AA
Venyaminova
Alya G
AG
D005456:Fluorescent Dyes / Q000737:chemistry*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000737:chemistry
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D042965:Methylenetetrahydrofolate Reductase (NADPH2)