title: Stress relaxation microscopy: imaging local stress in cells.
abstract: Biomechanics is gaining relevance as complementary discipline to structural and cellular biology. The response of cells to mechanical stimuli determines cell type and function, while the spatial distribution of mechanical forces within the cells is crucial to understand cell activity. The experimental methodologies to approach cell mechanics are diverse but either they are effective in few cases or they rule out the innate cell complexity. In this regard, we have developed a simple scanning probe-based methodology that overcomes the limitations of the available methods. Stress relaxation, the decay of the force exerted by the cell surface at constant deformation, has been used to extract relaxational responses at each cellular sublocalisation and generate maps. Surprisingly, decay curves exerted by test cells are fully described by a generalized viscoelastic model that accounts for more than one simultaneously occurring relaxations. Within the range of applied forces (0.5-4nN) a slow and a fast relaxation with characteristic times of 0.1 and 1s have been detected and assigned to rearrangements of cell membrane and cytoskeleton, respectively. Relaxation time mapping of entire cells is thus promising to simultaneously detect non-uniformities in membrane and cytoskeleton and as identifying tool for cell type and disease.
authors.affiliation: Biosurfaces unit, CIC BiomaGUNE, Paseo Miramón 182, 20009 San Sebastián-Donostia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Moreno-Flores
authors.forename: Rafael
authors.initials: R
authors.lastname: Benitez
authors.forename: Maria Dm
authors.initials: MD
authors.lastname: Vivanco
authors.forename: José Luis
authors.initials: JL
authors.lastname: Toca-Herrera
mesh_terms: D001696:Biomechanical Phenomena; D001943:Breast Neoplasms / Q000473:pathology / Q000503:physiopathology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000502:physiology; D002468:Cell Physiological Phenomena; D019245:Compressive Strength; D003599:Cytoskeleton / Q000502:physiology; D004548:Elasticity; D054459:Elasticity Imaging Techniques / Q000379:methods; D005260:Female; D006801:Humans; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D014783:Viscosity