Single amino acid changes in the turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards Arabidopsis thaliana mutants knocked out for eukaryotic initiation factors eIF(iso)4E and eIF(iso)4G.
Previous resistance analyses of Arabidopsis thaliana mutants knocked out for eukaryotic translation initiation factors showed that disruption of the At-eIF(iso)4E or both the At-eIF(iso)4G1 and At-eIF(iso)4G2 genes resulted in resistance against turnip mosaic virus (TuMV). This study selected TuMV virulent variants that overcame this resistance and showed that two independent mutations in the region coding for the viral genome-linked protein (VPg) were sufficient to restore TuMV virulence in At-eIF(iso)4E and At-eIF(iso)4G1xAt-eIF(iso)4G2 knockout plants. As a VPg-eIF(iso)4E interaction has been shown previously to be critical for TuMV infection, a systematic analysis of the interactions between A. thaliana eIF4Es and VPgs of virulent and avirulent TuMVs was performed. The results suggest that virulent TuMV variants may use an eIF4F-independent pathway.
Gallois
Jean-Luc
JL
Genetics and Breeding of Fruits and Vegetable, INRA Avignon, BP 94, F-84143 Montfavet, France.
Charron
Carine
C
Sánchez
Flora
F
Pagny
Gaëlle
G
Houvenaghel
Marie-Christine
MC
Moretti
André
A
Ponz
Fernando
F
Revers
Frédéric
F
Caranta
Carole
C
German-Retana
Sylvie
S
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000821:virology*; D029681:Arabidopsis Proteins / Q000235:genetics*; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics*; D039642:Eukaryotic Initiation Factors / Q000235:genetics*; D055786:Gene Knockout Techniques; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000235:genetics / Q000472:pathogenicity*; D025941:Protein Interaction Mapping; D013489:Suppression, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology
D029681:Arabidopsis Proteins; D039603:Eukaryotic Initiation Factor-4G; D039642:Eukaryotic Initiation Factors; D017361:Viral Nonstructural Proteins; D037521:Virulence Factors; C511734:eIF(iso)4E protein, Arabidopsis