Defining mechanisms that regulate RNA polymerase II transcription in vivo.
In the eukaryotic genome, the thousands of genes that encode messenger RNA are transcribed by a molecular machine called RNA polymerase II. Analysing the distribution and status of RNA polymerase II across a genome has provided crucial insights into the long-standing mysteries of transcription and its regulation. These studies identify points in the transcription cycle where RNA polymerase II accumulates after encountering a rate-limiting step. When coupled with genome-wide mapping of transcription factors, these approaches identify key regulatory steps and factors and, importantly, provide an understanding of the mechanistic generalities, as well as the rich diversities, of gene regulation.
Fuda
Nicholas J
NJ
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA.
Ardehali
M Behfar
MB
Lis
John T
JT
D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D014157:Transcription Factors; D012319:RNA Polymerase II