[The purification and characterization of a novel lipid transfer protein from caryopsis of barnyard grass (Echinochloa crusgalli)].
A novel lipid transfer protein called Ec-LTP was isolated from resting caryopsis of weed barnyard grass Echinochloa crusgalli (L.) Beauv.; its molecular weight, amino acid content and N-terminal amino acid sequence were determined. Ec-LTP was a 9150 Da protein, containing eight cysteine residues, which formed four disulfide bonds. The isolated protein could significantly inhibit the development of pathogenic fungi Phytophthora infestans and Helminthosporium sativum, causing the late blight of potato and tomato and the root rot of herbs, respectively.
Rogozhin
E A
EA
Odintsova
T I
TI
Musoliamov
A Kh
AKh
Smirnov
A N
AN
Babakov
A V
AV
Egorov
Ts A
TsA
Grishin
E V
EV
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D031747:Echinochloa / Q000737:chemistry* / Q000235:genetics / Q000382:microbiology; D006375:Helminthosporium; D055750:Phytophthora infestans; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D020539:Sequence Analysis, Protein
D002352:Carrier Proteins; D010940:Plant Proteins; C030363:lipid transfer protein