[Study on purification and property of polysaccharide from Scolopendra subspinipes mutilans].
OBJECTIVE
To obtain polysaccharide from Scolopendra subspinipes mutilans and study its partial properties.


METHODS
A raw polysaccharide was isolated from Chinese medicine Scolopendra subspinipes mutilans, and three groups were isolated by DEAE-52. The thin layer chromatography and gel filtration chromatography were used to detect the main monosaccharide composition and molecular weight of the group I. The inhibitory effect of the group I on tumor cells was detected by MTT assay.


RESULTS
The molecular weight of the group I was 33.1 kD. The inhibitory effect of the group I on Hela cells was obvious, as its inhibitive rate was 60.8% on Hela cells when the polysaccharide's concentration was 3.13 microg/mL, but it had no effect on the Eca 109 cells.


CONCLUSIONS
The group I has specific effects on different tumor cells.
Li
Xing-Nuan
XN
College of Medical, Jiujiang University, Jiujiang 332000, China. li_xingnuan@126.com
Xiao
Xiang
X
Wang
Yun
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001181:Arthropods / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D004938:Esophageal Neoplasms / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D008419:Materia Medica / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D008419:Materia Medica; D011134:Polysaccharides