[SKLB-102 inhibits acute hepatitis induced by concanavalin A in mice].
OBJECTIVE
To test the effect of 5-(4-methoxybenzylidene)-2-thioxo-dihydropyrimidine-4, 6 (1H, 5H)-dione (SKLB-102) on acute hepatic inflammatory induced by concanavalin A (ConA) in mice.


METHODS
The inhibitive effect of SKLB-102 on RAW264.7 cell migration induced by recombinant rat monocyte chemotactic protein-1 (MCP-1) was tested. The serum from the ConA-treated mice was collected after intragastric administration of SKLB-102 at the dose of 50 mg/kg bodyweight. The serum AST and ALT were determined by an automatic analyzer, and the serum TNF-alpha was determined with enzyme-linked immunosorbent assay (ELISA) kits. The liver samples were fixed in 10% formalin, embedded in paraffin, sectioned and then stained with hematoxylin and eosin for histological examinations.


RESULTS
SKLB-102 markedly reduced cell migrations, successfully reduced serum AST, ALT and down-regulated TNF-alpha.


CONCLUSIONS
SKLB-102 is likely to suppress the occurrence of Con A-induced hepatitis by suppressing macrophages migration and TNF-alpha releases.
He
Chun-Mei
CM
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Cai
Lu-Lu
LL
Zheng
Hao
H
Ma
Liang
L
Luo
You-Fu
YF
Chen
Li-Juan
LJ
D000208:Acute Disease; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D003208:Concanavalin A / Q000633:toxicity*; D008099:Liver / Q000473:pathology; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D045162:Thiazolidinediones / Q000627:therapeutic use*
C546799:5-(4-methoxybenzylidene)thiazolidine-2,4-dione; D045162:Thiazolidinediones; D003208:Concanavalin A