title: [Effect of electroacupuncture on epileptic EEG and intracellular Ca2+ content in the hippocampus in epilepsy rats].
abstract: OBJECTIVE
  To study the underlying mechanism of electroacupuncture (EA) in relieving epilepsy in pentylenetetrazole (PTZ)-induced epilepsy rats.
  
  
  METHODS
  Twenty SD rats were randomly divided into normal control, model, EA, Nimodipine groups, with 5 cases in each. Epilepsy model was established by intraperitoneal injection of PTZ (32.0 mg/kg), once daily for 28 days. EA (100 Hz, 0.6 mA) was applied to "Baihui" (GV 20) and "Dazhui" (GV 14), once daily for 7 days. For Nimodipine group, the rats were given with nimodipine (0.25 mg/kg, i.p.), once daily for 7 days. Electroencephalogram (EEG) was recorded and the fluorescence intensity of Ca2+ of the hippocampus tissue sections was detected by laser scanning confocal microscope (LSCM) after incubation in artificial cerebrospinal fluid containing Flou-3/AM (10 micromol/L) and pluronic F-127 (5 microl).
  
  
  RESULTS
  Compared with model group, the latencies of epileptic EEG seizure prolonged obviously (P < 0.05), and epileptic EEG seizure frequencies decreased significantly (P < 0.05) in EA and Nimodipine groups. The fluorescence intensity of intracellular Ca2+ in hippocampus tissue in model group was obviously higher than that in control group (P < 0.01). In comparison to model group, Ca2+ levels in EA and Nimodipine groups lowered considerably (P < 0.05, P < 0.01). No significant differences were found between EA and Nimodipine groups in the aforementioned 3 indexes (P > 0.05).
  
  
  CONCLUSIONS
  EA has an obvious anti-epileptic effect, which may be closely related to its effect in downregulating the increased hippocampal Ca2+ level in PTZ-kindled epilepsy rats.
authors.affiliation: Shanghai Institute of Acu-moxibustion and Meridians, Shanghai 200030, China. saildoctor@126.com
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Guo-long
authors.initials: GL
authors.lastname: Xu
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Yang
authors.forename: De-kai
authors.initials: DK
authors.lastname: Shen
authors.forename: Pin-zhi
authors.initials: PZ
authors.lastname: Feng
authors.forename: Pin
authors.initials: P
authors.lastname: Wang
authors.forename: Xiang-guo
authors.initials: XG
authors.lastname: Liu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004195:Disease Models, Animal; D015671:Electroacupuncture; D004569:Electroencephalography; D004827:Epilepsy / Q000378:metabolism / Q000628:therapy*; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats
chemical_list: D002118:Calcium