Induction of JAM-A during differentiation of human THP-1 dendritic cells.
Junctional adhesion molecule (JAM)-A is not only localized at tight junctions of endothelial and epithelial cells but is also expressed on circulating leukocytes and dendritic cells (DCs). In the present study, to investigate the regulation of JAM-A in DCs, mature DCs were differentiated from the human monocytic cell THP-1 by treatment with IL-4, GM-CSF, TNF-alpha, and ionomycin, and some cells were pretreated with the PPAR-gamma agonists. In the THP-1 monocytes, mRNAs of tight junction molecules, occludin, tricellulin, JAM-A, ZO-1, ZO-2 and claudin-4, -7, -8, and -9 were detected by RT-PCR. In mature DCs that had elongated dendrites, mRNA and protein of JAM-A were significantly increased compared to the monocytes. PPAR-gamma agonists prevented the elongation of dentrites but not upregulation of JAM-A in mature DCs. These findings indicated that the induction of JAM-A occurred during differentiation of human THP-1 DCs and was independent of PPAR-gamma and the p38 MAPK pathway.
Ogasawara
Noriko
N
Department of Otolaryngology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan.
Kojima
Takashi
T
Go
Mitsuru
M
Fuchimoto
Jun
J
Kamekura
Ryuta
R
Koizumi
Jun-ichi
J
Ohkuni
Tsuyoshi
T
Masaki
Tomoyuki
T
Murata
Masaki
M
Tanaka
Satoshi
S
Ichimiya
Shingo
S
Himi
Tetsuo
T
Sawada
Norimasa
N
D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002454:Cell Differentiation; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D047495:PPAR gamma / Q000819:agonists / Q000378:metabolism; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D007136:Immunoglobulins; D047495:PPAR gamma; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D015759:Ionomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D048051:p38 Mitogen-Activated Protein Kinases