title: Identification of recombinant human papillomavirus type 16 variants.
abstract: Intratypic diversity of human papillomavirus (HPV) genome is generally characterized by point mutation, insertion, and/or deletion. Using PCR-based cloning and sequencing, we detected concurrent infection with 8 HPV16 variants in a woman enrolled in the ASCUS-LSIL Triage Study. The European variant was the major variant; each of the 7 minor variants had partial DNA sequences identical to the European variant and another part identical to the African 2 variant. At a follow-up visit, only an HPV16 African 2 variant was detected. Results from the present study suggest presence of intratypic recombination of HPV genome in natural infection.
authors.affiliation: Department of Pathology, School of Medicine, Seattle, WA 98105, USA.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Jiang
authors.forename: Long Fu
authors.initials: LF
authors.lastname: Xi
authors.forename: Zoe R
authors.initials: ZR
authors.lastname: Edelstein
authors.forename: Denise A
authors.initials: DA
authors.lastname: Galloway
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Olsem
authors.forename: William Chun-Che
authors.initials: WC
authors.lastname: Lin
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Kiviat
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004279:DNA, Viral