Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses.
NAC family genes encode plant-specific transcription factors involved in diverse biological processes. In this study, the Arabidopsis NAC gene ATAF1 was found to be induced by drought, high-salinity, abscisic acid (ABA), methyl jasmonate, mechanical wounding, and Botrytis cinerea infection. Significant induction of ATAF1 was found in an ABA-deficient mutant aba2 subjected to drought or high salinity, revealing an ABA-independent mechanism of expression. Arabidopsis ATAF1-overexpression lines displayed many altered phenotypes, including dwarfism and short primary roots. Furthermore, in vivo experiments indicate that ATAF1 is a bona fide regulator modulating plant responses to many abiotic stresses and necrotrophic-pathogen infection. Overexpression of ATAF1 in Arabidopsis increased plant sensitivity to ABA, salt, and oxidative stresses. Especially, ATAF1 overexpression plants, but not mutant lines, showed remarkably enhanced plant tolerance to drought. Additionally, ATAF1 overexpression enhanced plant susceptibility to the necrotrophic pathogen B. cinerea, but did not alter disease symptoms caused by avirulent or virulent strains of P. syringae pv tomato DC3000. Transgenic plants overexpressing ATAF1 were hypersensitive to oxidative stress, suggesting that reactive oxygen intermediates may be related to ATAF1-mediated signaling in response to both pathogen and abiotic stresses.
Wu
Yaorong
Y
State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
Deng
Zhiyong
Z
Lai
Jianbin
J
Zhang
Yiyue
Y
Yang
Cuiping
C
Yin
Bojiao
B
Zhao
Qingzhen
Q
Zhang
Ling
L
Li
Yin
Y
Yang
Chengwei
C
Xie
Qi
Q
D000040:Abscisic Acid; D000085:Acetates; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020171:Botrytis / Q000472:pathogenicity; D003517:Cyclopentanes; D055864:Droughts; D018384:Oxidative Stress / Q000502:physiology; D054883:Oxylipins; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018517:Plant Roots / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000472:pathogenicity; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D055051:Salt-Tolerant Plants / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology
C517610:ATAF1 protein, Arabidopsis; D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D012097:Repressor Proteins; D000040:Abscisic Acid; C072239:methyl jasmonate