Chromosome-wide analysis of protein binding and modifications.
In order to fully understand the functions of a DNA-binding protein it is necessary to identify all of its binding sites in chromosomes and assess the role of each site in the overall biological function of the factor. An approach ChIP-on-Chip which combines the chromatin immunoprecipitation technique with chromosomal DNA microarray analysis, has proven to be a powerful means for the chromosome-wide identification of protein binding sites. This approach can also be used to characterize chromosome-wide variations in patterns of post-translational protein modifications, for example histone modifications. This chapter presents methodologies for the ChIP-on-Chip analysis, using as an example the identification of chromosome-wide binding sites for the TATA-binding protein in mitotic cells.
Sarge
Kevin D
KD
Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY, USA.
Xing
Hongyan
H
Park-Sarge
Ok-Kyong
OK
D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D002877:Chromosomes, Human; D017931:DNA Primers; D005456:Fluorescent Dyes; D006801:Humans; D011485:Protein Binding
D017931:DNA Primers; D005456:Fluorescent Dyes