Classification of individuals with dyslipidaemia controlled by statins according to plasma biomarkers of oxidative stress using cluster analysis.
Oxidative stress is a physiological condition that is associated with atherosclerosis, and it can be influenced by diet. Our objective was to group fifty-seven individuals with dyslipidaemia controlled by statins according to four oxidative biomarkers, and to evaluate the diet pattern and blood biochemistry differences between these groups. Blood samples were collected and the following parameters were evaluated: diet intake; plasma fatty acids; lipoprotein concentration; glucose; oxidised LDL (oxLDL); malondialdehyde (MDA); total antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ability power assays. Individuals were separated into five groups by cluster analysis. All groups showed a difference with respect to at least one of the four oxidative stress biomarkers. The separation of individuals in the first axis was based upon their total antioxidant activity. Clusters located on the right side showed higher total antioxidant activity, higher myristic fatty acid and lower arachidonic fatty acid proportions than clusters located on the left side. A negative correlation was observed between DPPH and the peroxidability index. The second axis showed differences in oxidation status as measured by MDA and oxLDL concentrations. Clusters located on the upper side showed higher oxidative status and lower HDL cholesterol concentration than clusters located on the lower side. There were no differences in diet among the five clusters. Therefore, fatty acid synthesis and HDL cholesterol concentration seem to exert a more significant effect on the oxidative conditions of the individuals with dyslipidaemia controlled by statins than does their food intake.
Botelho
Patrícia B
PB
Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580 B14, 05508-900 São Paulo, SP, Brazil.
Fioratti
Cyntia O
CO
Abdalla
Dulcinéia S P
DS
Bertolami
Marcelo C
MC
Castro
Inar A
IA
D000368:Aged; D015415:Biomarkers / Q000097:blood; D001713:Biphenyl Compounds / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D016000:Cluster Analysis; D004032:Diet; D050171:Dyslipidemias / Q000097:blood* / Q000145:classification; D005227:Fatty Acids / Q000097:blood; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D010851:Picrates / Q000097:blood
D015415:Biomarkers; D001713:Biphenyl Compounds; D001786:Blood Glucose; D005227:Fatty Acids; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D010851:Picrates; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl