title: Bioburden assessment of banked bone used for allografts.
abstract: Allograft bone is commonly used in reconstructive orthopaedic surgery and needs to be assessed for bioburden before transplant. The Microbiology Department of the South Eastern Area Laboratory Services (SEALS), located at the St. George Hospital, Sydney, has provided this service to the New South Wales (NSW) Bone Bank. This study reviewed the organisms isolated from femoral head allografts of living donors from the NSW Bone Bank over a 7-year period. It was found that growth was reported from 4.9% of samples with the predominant organism being coagulase-negative staphylococci. This review will focus on the micro-organisms isolated, the interaction of the laboratory with the bone bank, the relevance of the bioburden assessment in the overall quality process and patient safety.
authors.affiliation: South Eastern Area Laboratory Services, St. George Hospital, Sydney, NSW, Australia. Kerry.Varettas@sesiahs.health.nsw.gov.au
authors.forename: Kerry
authors.initials: K
authors.lastname: Varettas
authors.forename: Peter
authors.initials: P
authors.lastname: Taylor
mesh_terms: D001419:Bacteria / Q000302:isolation & purification; D018586:Bone Banks / Q000592:standards; D016025:Bone Transplantation / Q000009:adverse effects; D005270:Femur Head / Q000382:microbiology* / Q000637:transplantation*; D005658:Fungi / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D046509:Tissue Culture Techniques; D014184:Transplantation, Homologous