Involvement of non-enzymatic antioxidant defenses in the protective effect of diphenyl diselenide on testicular damage induced by cadmium in mice.
The involvement of non-enzymatic antioxidant defenses in the protective effect of diphenyl diselenide (PhSe)(2) on testicular damage caused by cadmium in mice was investigated. Mice received a single dose of CdCl(2) (5mg/kg, intraperitoneally). Thirty minutes after the CdCl(2) injection, they received a single oral dose of (PhSe)(2) (400micromol/kg). Twenty-four hours after CdCl(2) administration, blood samples were collected and mice were killed and had their testes dissected. Parameters in plasma (aspartate (AST) and alanine (ALT) aminotransferases and lactato dehydrogenase (LDH) activities as well as creatinine levels) were determined. The activity of delta-aminolevulinate dehydratase (delta-ALA-D), the levels of thiobarbituric acid-reactive substances (TBARS), ascorbic acid and nonprotein thiols (NPSH) and histological analysis were determined in collected samples. Results demonstrated that (PhSe)(2) protected against toxicity induced by CdCl(2) on delta-ALA-D activity, ascorbic acid and NPSH levels. (PhSe)(2) protected against the increase in plasma AST, ALT and LDH activities caused by CdCl(2). Testes of mice exposed to CdCl(2) showed marked histopathological alterations that were ameliorated by administration of (PhSe)(2). (PhSe)(2) protected against toxicity induced by CdCl(2) in testes of mice. Ascorbic acid and NPSH, non-enzymatic antioxidant defenses, are involved in the protective effect of (PhSe)(2) against testicular damage caused by CdCl(2) in mice.
Brandão
Ricardo
R
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, RS, Brazil.
Santos
Francielli Weber
FW
Oliveira
Renata
R
Roman
Silvane Souza
SS
Nogueira
Cristina Wayne
CW
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis; D001555:Benzene Derivatives / Q000494:pharmacology*; D002104:Cadmium / Q000633:toxicity*; D019256:Cadmium Chloride / Q000008:administration & dosage; D004796:Clinical Enzyme Tests; D003404:Creatinine / Q000097:blood; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D009929:Organ Size; D016566:Organoselenium Compounds / Q000494:pharmacology*; D000623:Porphobilinogen Synthase / Q000378:metabolism; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology; D013438:Sulfhydryl Compounds / Q000032:analysis; D013733:Testicular Diseases / Q000139:chemically induced / Q000517:prevention & control*; D013737:Testis / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology
D000975:Antioxidants; D001555:Benzene Derivatives; D016566:Organoselenium Compounds; D013438:Sulfhydryl Compounds; D002104:Cadmium; C061132:diphenyldiselenide; D003404:Creatinine; D000623:Porphobilinogen Synthase; D019256:Cadmium Chloride; D001205:Ascorbic Acid