Construction of collagen II/hyaluronate/chondroitin-6-sulfate tri-copolymer scaffold for nucleus pulposus tissue engineering and preliminary analysis of its physico-chemical properties and biocompatibility.
To construct a novel scaffold for nucleus pulposus (NP) tissue engineering, The porous type II collagen (CII)/hyaluronate (HyA)-chondroitin-6-sulfate (6-CS) scaffold was prepared using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) cross-linking system. The physico-chemical properties and biocompatibility of CII/HyA-CS scaffolds were evaluated. The results suggested CII/HyA-CS scaffolds have a highly porous structure (porosity: 94.8 +/- 1.5%), high water-binding capacity (79.2 +/- 2.8%) and significantly improved mechanical stability by EDC/NHS crosslinking (denaturation temperature: 74.6 +/- 1.8 and 58.1 +/- 2.6 degrees C, respectively, for the crosslinked scaffolds and the non-crosslinked; collagenase degradation rate: 39.5 +/- 3.4 and 63.5 +/- 2.0%, respectively, for the crosslinked scaffolds and the non-crosslinked). The CII/HyA-CS scaffolds also showed satisfactory cytocompatibility and histocompatibility as well as low immunogenicity. These results indicate CII/HyA-CS scaffolds may be an alternative material for NP tissue engineering due to the similarity of its composition and physico-chemical properties to those of the extracellular matrices (ECM) of native NP.
Li
Chang-Qing
CQ
Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, People's Republic of China.
Huang
Bo
B
Luo
Gang
G
Zhang
Chuan-Zhi
CZ
Zhuang
Ying
Y
Zhou
Yue
Y
D001672:Biocompatible Materials / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry*; D024043:Collagen Type II / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007403:Intervertebral Disc / Q000166:cytology* / Q000502:physiology*; D008422:Materials Testing; D010865:Pilot Projects; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D024043:Collagen Type II; D011108:Polymers; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates