[The effect of the parameters of biolistic transformation of spring barley (Hordeum vulgare L.) on the level of transient expression of GFP reporter gene].
The effect of the parameters of biolistic transformation (rupture disk pressure of helium, vacuum pressure, stopping screen to target tissue distance, material (gold or tungsten) and size of particles, and duration of explant culturing before bombardment) on the level of transient expression of GFP reporter gene was studied in barley embryos. The highest transient expression was observed after explant preincubation for 12-14 days and bombardment with 1 microm gold particles at the helium pressure of 61.24-74.85 atm, vacuum pressure of 0.064 atm, and distance to target of 9 cm.
Chernobrovkina
M A
MA
Sidorov
E A
EA
Baranov
I A
IA
Kharchenko
P N
PN
Dolgov
S V
SV
D019470:Biolistics / Q000379:methods*; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006371:Helium; D001467:Hordeum / Q000235:genetics*; D030821:Plants, Genetically Modified; D011312:Pressure; D012639:Seeds / Q000235:genetics; D014170:Transformation, Genetic
D049452:Green Fluorescent Proteins; D006371:Helium