title: Simultaneous determination of seven components in Qibaomeiran pill by HPLC-DAD.
abstract: A high-performance liquid chromatography coupled with photo diode array detection method is developed for simultaneous determination of seven active components in Qibaomeiran pill, including rutin, 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glycoside, ferulic acid, psoralen, isopsoralen, emodin, and physcion. The analysis is performed on a C(18) column using a mobile phase composed of A (0.1% acetic acid) and B (acetonitrile) with linear gradient elution. Four wavelengths at 245, 290, 320, and 350 nm were chosen as the monitoring wavelength to determine seven active components, respectively. All the compounds show good linearity (r > 0.999). The developed method is fully validated in respect to precision, repeatability, and accuracy. The proposed method is successfully applied to quantify the seven active components in different Qibaomeiran pill samples. The results indicate that the developed assay could be considered as a suitable quality control method for the Qibaomeiran pill.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, PR China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Fengli
authors.initials: F
authors.lastname: Xie
authors.forename: Lantong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D011564:Furocoumarins / Q000032:analysis / Q000737:chemistry; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results
chemical_list: D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; D006027:Glycosides; C000597912:qibaomeiran; C004999:ferulic acid; C011659:angelicin; C008905:physcione; D004642:Emodin