A photochemical detection of methylcytosine by using hydrophobic interaction.
In this paper, the nonenzymatic detection of 5-methylcytosine with high selectivity is described. We present a new methylation detection method by using template-directed photoligation through 5-cyanovinyl-2'-deoxyuridine ((C)U). Significantly, the photoligation yield of the 5-methylcytosine case was approximately 9.1-fold higher than that in the case of cytosine.
Ami
Takehiro
T
School of Materials Science, Japan Advanced Institute of Science and Technnology, Nomi, Ishikawa 923-1292, Japan.
Ogino
Masayuki
M
Taya
Yuuta
Y
Takemura
Yumiko
Y
Fujimoto
Kenzo
K
D044503:5-Methylcytosine / Q000032:analysis* / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D055668:Photochemical Processes; D010777:Photochemistry / Q000379:methods
D009838:Oligodeoxyribonucleotides; D044503:5-Methylcytosine; C032387:5-(2-cyanovinyl)-2'-deoxyuridine; D003857:Deoxyuridine