[Regulation of protein kinases on signal pathway in breast cancer cell MCF-7 by curcumin].
OBJECTIVE
To observe the effect of curcumin on protein kinases on cell signal pathway in breast cancer cell MCF-7.


METHODS
The suppressive effect of curcumin on MCF-7 cell proliferation as different concentration and at different time points were observed by MTT assay; The activity of p-ERK, p-NF-kappaB, p-p38 and p-JNK were observed by western blot.


RESULTS
The suppressive effect of curcumin was shown as a dose dependent and a time dependent manner,and the IC50 was 22.48 micromol/L 40 micromol/L curcumin inhibited the expression of p-ERK, p-NF-kappaB and p-p38, but not p-JNK.


CONCLUSIONS
Curcumin can inhibit MCF-7 cell's proliferation, and its mechanism may be related to the activities of protein kinases on cell signal pathway.
Zhou
Qian-Mei
QM
Shanghai University of TCM, Shanghai 201203, China.
Su
Shi-Bing
SB
Zhang
Hui
H
Lu
Yi-Yu
YY
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004791:Enzyme Inhibitors; D016328:NF-kappa B; D011505:Protein-Tyrosine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D003474:Curcumin