ENU induced single mutation locus on chr 16 leads to high-frequency hearing loss in mice.
The hallmark of age-related (presbycusis) and noise-induced hearing loss is high-frequency (> 20 kHz) hearing loss. Through a collaborative study with TMGC (Tennessee Mouse Genome Consortium), seventeen ENU-induced mouse mutation strains with high-frequency hearing loss have been identified, but affected genes are yet identified. As a first step in identifying the gene/s underlying the ENU mutations, we created a F2 population between a representative mutation strain, 118 TNE and a wild type strain, CAST/EJ (CAST). Phenotypic analysis showed that there is a 3:1 ratio of segregation between normal and hearing loss in the F2 population, suggestion a single locus regulation. However, the linkage mapping identified 2 QTLs, each on chromosomes 15 and 16. Further statistical analysis of marker segregation patterns revealed that the locus on Chr 16 was ENU induced while the one on Chr 15 was derived from the parental strain, CAST.
Jiao
Yan
Y
Departments of Orthopaedic Surgery-Campbell Clinic and Pathology, University of Tennessee Health Science Center (UTHSC), Memphis, TN, USA.
Cai
Chun
C
Kermany
Mohammad Habiby
MH
Yan
Jian
J
Cai
Qing
Q
Miller
Darla
D
Goldowitz
Daniel
D
Li
Xinmin
X
Yoo
Tai-June
TJ
Gu
Weikuan
W
D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000737:chemistry / Q000187:drug effects*; D003433:Crosses, Genetic; D005038:Ethylnitrosourea / Q000633:toxicity*; D005819:Genetic Markers / Q000502:physiology; D006316:Hearing Loss, High-Frequency / Q000139:chemically induced* / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009154:Mutation / Q000187:drug effects / Q000502:physiology*; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000187:drug effects
D005819:Genetic Markers; D005038:Ethylnitrosourea