Development of a rapid and reversible photocrosslinking of RNA.
We describe a novel reversible interstrand photocrosslinking reaction of RNA. In this system, a modified oligodeoxynucleotide (ODN) containing 3-cyanovinylcarbazole nucleoside ((CNV)K) reacts by photoirradiation at 366 nm for 1 s with pyrimidine residue of a complementary template RNA to yield a crosslinked product in 94% yield.
Yoshimura
Yoshinaga
Y
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan.
Ohtake
Tomoko
T
Okada
Hajime
H
Fujimoto
Kenzo
K
D002227:Carbazoles / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects; D055668:Photochemical Processes; D012313:RNA / Q000737:chemistry* / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002227:Carbazoles; D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA