[In vitro activity of matrine against Candida albicans biofilms].
OBJECTIVE
To establish a model of Candida albicans biofilms and to examine the effect of matrine on C.albicans biofilms and ultrastructure.


METHODS
C. albicans collection strain ATCC76615 was obtained and propagated. Biofilms were formed in 96-well microtiter plates. Antifungal susceptibility testing of C. albicans biofilms were assessed with the tetrazolium salt (XTT) reduction assay. Confocal laser scanning microscopy (CLSM) and dead/live fluorescent staining technique were combined to detect the effects of Matrine on preformed C. albican biofilms' composition and ultrastructure.


RESULTS
Matrine was active against different growth stages (early,middle,mature) of biofilms; The bioactivity and drug-resistance of C. albican biofilm increased with culturing time. CLSM showed that C. albicans biofilms were inhibited and growth were predominantly composed of yeast cells and pseudohyphae.


CONCLUSIONS
This study demonstrates that Matrine has potent activity against C.albicans biofilms in vitro and potential therapeutic implication for biofilm-associated candidal infections.
Wu
Lan
L
Department of Oral Medicine, College of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. teana-wu@hotamil.com
Zhou
Zeng-tong
ZT
Zhou
Yong-mei
YM
Wang
Hai-yan
HY
Shi
Lin-jun
LJ
D000470:Alkaloids; D000935:Antifungal Agents; D018441:Biofilms; D002176:Candida albicans; D006801:Humans; D066298:In Vitro Techniques; D011807:Quinolizines; D000093842:Matrines
D000470:Alkaloids; D000935:Antifungal Agents; D011807:Quinolizines; D000093842:Matrines