[The use of enzymatic preparation for the production of low molecular-weight chitosan from the king crab hepatopancrease].
This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 100 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1:200, 37 degrees C, and 20 h duration of hydrolysis.
Il'ina
A V
AV
Zagorskaia
D S
DS
Levov
A N
AN
Albulov
A I
AI
Kovacheva
N P
NP
Varlamov
V P
VP
D000818:Animals; D033381:Anomura / Q000737:chemistry* / Q000201:enzymology; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry* / Q000302:isolation & purification*; D043143:Hepatopancreas / Q000737:chemistry / Q000201:enzymology; D006868:Hydrolysis; D008970:Molecular Weight
D048271:Chitosan