title: Methionine sulfoxide reductase A expression is regulated by the DAF-16/FOXO pathway in Caenorhabditis elegans.
abstract: The methionine sulfoxide reductase system has been implicated in aging and protection against oxidative stress. This conserved system reverses the oxidation of methionine residues within proteins. We analyzed one of the components of this system, the methionine sulfoxide reductase A gene, in Caenorhabditis elegans. We found that the msra-1 gene is expressed in most tissues, particularly in the intestine and the nervous system. Worms carrying a deletion of the msra-1 gene are more sensitive to oxidative stress, show chemotaxis and locomotory defects, and a 30% decrease in median survival. We established that msra-1 expression decreases during aging and is regulated by the DAF-16/FOXO3a transcription factor. The absence of this enzyme decreases median survival and affects oxidative stress resistance of long lived daf-2 worms. A similar effect of MSRA-1 absence in wild-type and daf-2 (where most antioxidant enzymes are activated) backgrounds, suggests that the lack of this member of the methionine repair system cannot be compensated by the general antioxidant response. Moreover, FOXO3a directly activates the human MsrA promoter in a cell culture system, implying that this could be a conserved mechanism of MsrA regulation. Our results suggest that repair of oxidative damage in proteins influences the rate at which tissues age. This repair mechanism, rather than the general decreased of radical oxygen species levels, could be one of the main determinants of organisms' lifespan.
authors.affiliation: Centro de Envejecimiento y Regeneración, Centro de Regulación Celular y Patología Joaquin V. Luco, Santiago, Chile.
authors.forename: Alicia N
authors.initials: AN
authors.lastname: Minniti
authors.forename: Romina
authors.initials: R
authors.lastname: Cataldo
authors.forename: Carla
authors.initials: C
authors.lastname: Trigo
authors.forename: Luis
authors.initials: L
authors.lastname: Vasquez
authors.forename: Patricio
authors.initials: P
authors.lastname: Mujica
authors.forename: Federico
authors.initials: F
authors.lastname: Leighton
authors.forename: Nibaldo C
authors.initials: NC
authors.lastname: Inestrosa
authors.forename: Rebeca
authors.initials: R
authors.lastname: Aldunate
mesh_terms: D020121:5' Untranslated Regions; D000375:Aging; D000818:Animals; D001522:Behavior, Animal; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008124:Locomotion; D051150:Methionine Sulfoxide Reductases; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D029742:Caenorhabditis elegans Proteins; C546325:FOXO3a protein, C elegans; D051858:Forkhead Transcription Factors; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase