Parapedobacter luteus sp. nov. and Parapedobacter composti sp. nov., isolated from cotton waste compost.
Two aerobic, Gram-reaction-negative, non-spore-forming bacterial strains, 4M29T and 4M40T, were isolated from cotton composts. The two strains grew in the presence of 0-5% (w/v) NaCl (optimum growth in the absence of NaCl), at pH 6.0-8.0 (optimum, pH 7.0) and at 15-45 degrees C (optimum, 30 degrees C). The strains shared 97.1% 16S rRNA gene sequence similarity. Strains 4M29T and 4M40T showed the next highest levels of 16S rRNA gene sequence similarity to Parapedobacter koreensis Jip14T (95.6 and 94.4%, respectively) and Parapedobacter soli DCY14T (95.2 and 93.8%). The level of DNA-DNA relatedness between strains 4M29T and 4M40T was 38%. The two strains contained iso-C15:0, summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1omega7c) and iso-C17:0 3-OH as major fatty acids, MK-7 as the major respiratory quinone, homospermidine as the only polyamine and phosphatidylethanolamine as the major polar lipid. The DNA G+C contents of strains 4M29T and 4M40T were 47.6 and 48.6 mol%, respectively. On the basis of phylogenetic and phenotypic data, strains 4M29T and 4M40T are considered to represent two novel species of the genus Parapedobacter, for which the names Parapedobacter luteus sp. nov. (type strain 4M29T =KACC 10955T =JCM 15977T) and Parapedobacter composti sp. nov. (type strain 4M40T =KACC 10972T =JCM 15978T) are proposed.
Kim
Soo-Jin
SJ
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Suwon 441-707, Republic of Korea.
Weon
Hang-Yeon
HY
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Suwon 441-707, Republic of Korea.
Kim
Yi-Seul
YS
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Suwon 441-707, Republic of Korea.
Yoo
Seung-Hee
SH
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Suwon 441-707, Republic of Korea.
Kim
Byung-Yong
BY
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Suwon 441-707, Republic of Korea.
Anandham
Rangasamy
R
Agricultural Microbiology, Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai, India.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Suwon 441-707, Republic of Korea.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D003368:Gossypium / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014866:Waste Products / Q000032:analysis*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014866:Waste Products