title: Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis.
abstract: A prospective study was designed to evaluate the clinical usefulness of spoligotyping applied directly to sputum samples. Patients suspected of having tuberculosis were recruited at the Hospital Sanatorio Partenon in Porto Alegre, Brazil. Of the 197 samples included in the analysis, 175 (88.8%) yielded a spoligotyping result that fully matched that obtained from culture. Low bacillary samples presented lower accuracy (50%). From 135 Mycobacterium tuberculosis spoligopatterns, we identified 44 different spoligotypes, of which 21 were shared patterns and 23 were unique. T1 was the most frequent subfamily. The genotyping strategy proposed here presents a short turnaround time and could be helpful in providing rapid information on strain identities in a clinical setting.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600, Porto Alegre, RS 90035-003, Brazil. patricia_cafrune@hotmail.com
authors.forename: Patricia Izquierdo
authors.initials: PI
authors.lastname: Cafrune
authors.forename: Lia Gonçalves
authors.initials: LG
authors.lastname: Possuelo
authors.forename: Andrezza Wolowski
authors.initials: AW
authors.lastname: Ribeiro
authors.forename: Marta Osório
authors.initials: MO
authors.lastname: Ribeiro
authors.forename: Gisela
authors.initials: G
authors.lastname: Unis
authors.forename: Carla Adriane
authors.initials: CA
authors.lastname: Jarczewski
authors.forename: Maria Lúcia Rosa
authors.initials: ML
authors.lastname: Rossetti
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Zaha
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D011446:Prospective Studies; D013183:Sputum / Q000737:chemistry / Q000382:microbiology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial