Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10 degrees C with electrolyte free medium.
OBJECTIVE
In-situ preservation of cauda epididymal spermatozoa at -10 degrees C with electrolyte free media for obtaining maximum functional gametes than preservation at 5 degrees C.


METHODS
Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 - glycerol and soybean lecithin - Coenzyme Q10- glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degrees C, wrapped with aluminium foil and freezed at - 10 degrees C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5 degrees C in a water bath for 5 min.


RESULTS
Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 +/- 0.62; 45.2 +/- 0.82 and 41.6 +/- 0.79 percent respectively on day 21.


CONCLUSIONS
This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter.
Datta
Uttam
U
Department of Veterinary Gynaecology & Obstetrics, Faculty of Veterinary and Animal Sciences West Bengal University of ANIMAL and Fishery Sciences, 37and 68, Kshudiram Bose Sarani, Belgachia, Kolkata, 700037, West Bengal, India. uttamdtt@yahoo.com
Chandra Sekar
M
M
Hembram
Manik Lal
ML
Dasgupta
Raju
R
D000818:Animals; D002470:Cell Survival; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D003470:Culture Media / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D004822:Epididymis / Q000166:cytology*; D005990:Glycerol / Q000737:chemistry; D006041:Goats; D054709:Lecithins / Q000737:chemistry; D008297:Male; D009994:Osmolar Concentration; D012662:Semen Preservation / Q000379:methods*; D013025:Glycine max / Q000737:chemistry; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
D003451:Cryoprotective Agents; D003470:Culture Media; D004573:Electrolytes; D054709:Lecithins; D014451:Ubiquinone; C024989:coenzyme Q10; D005990:Glycerol