Fast and simple method for the determination of urinary 1-hydroxypyrene.
A fast method for the measurement of metabolites of pyrene in urine was improved by HPLC with fluorescence detector using "heart-cut" technique. This method can quantify the total amount of pyrene metabolites corresponding to glucuronic acid and sulfate conjugates as well as free 1-OH-Py. The hydrolyzed biological fluid was directly injected into the chromatographic system, via a column-switching system. Pre-treatment and analysis were performed within 0.5 and 9 min, respectively. Enzymatic hydrolysis has been optimized to not exceed 2 h. The best response function, in the 0.2-10 ng/mL range, is the linear regression, bringing simplicity, good accuracy, and low LOQ. The intra- and interday precision values were inferior to 1 and 2%, respectively. The proposed method provided a simple, convenient, and practical procedure to determine the level of the main urinary pyrene metabolites in biological samples.
Cahours
Xavier
X
SEITA, Imperial Tobacco Group, Fleury-les-Aubrais, France. xavier.cahours@altadis.com
Blanchet
Maryline
M
Rey
Miguel
M
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006868:Hydrolysis; D009153:Mutagens / Q000302:isolation & purification / Q000378:metabolism; D011721:Pyrenes / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D014556:Urine / Q000737:chemistry*
D009153:Mutagens; D011721:Pyrenes; C033146:1-hydroxypyrene