title: High-performance liquid chromatographic method for the determination of cyromazine and melamine residues in milk and pork.
abstract: A high-performance liquid chromatography method is described in this paper. The method uses NH(2) column and 97% acetonitrile eluate to determine the insecticide cyromazine and metabolite melamine residues in milk and pork. Samples were treated with NaOH and extracted with acetonitrile containing 20% NH(4)OH. Target analytes of samples were cleaned up and concentrated by C(18) column solid-phase extraction. A separation for cyromazine and melamine was achieved, and respective retention times were 8 and 12 min. The calibration curves for cyromazine and melamine were linear in a concentration range of 0.01-1.0 microg/mL, with correlation coefficients of 0.9999 and 0.9997, respectively. The limit of detection of both compounds was 0.2 ng, and the limit of quantitation was 0.02 mg/kg. Recoveries of cyromazine and melamine at fortified levels of 0.02, 0.05, and 0.1 mg/kg ranged from 84.5-90.8%, and 83.6-91.3%, respectively, with coefficient of variation of 3.1-7.8%.
authors.affiliation: Jiangsu Province Key Laboratory of Animal-Derived Food Safety, Jiangsu Academy of Agricultural Science, Jiangsu, Nanjing 210014, P.R. China.
authors.forename: Ruicheng
authors.initials: R
authors.lastname: Wei
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Zeng
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Tiezheng
authors.initials: T
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D014227:Triazines / Q000032:analysis*
chemical_list: D010573:Pesticide Residues; D014227:Triazines; C028704:cyromazine; C011907:melamine