Control of the release of digestive enzymes in the larvae of the fall armyworm, Spodoptera frugiperda.
There is a basal level of enzyme activity for trypsin, aminopeptidase, amylase, and lipase in the gut of unfed larval (L6) Spodoptera frugiperda. Trypsin activity does not decrease with non-feeding, possibly because of the low protein levels in plants along with high amino acid requirements for growth and storage (for later reproduction in adults). Therefore, trypsin must always be present so that only a minimal protein loss via egestion occurs. Larvae, however, adjust amylase activity to carbohydrate ingestion, and indeed amylase activity is five-fold higher in fed larvae compared to unfed larvae. Gut lipase activity is low, typical of insects with a high carbohydrate diet. A flat-sheet preparation of the ventriculus was used to measure the release of enzymes in response to specific nutrients and known brain/gut hormones in S. frugiperda. Sugars greatly increase (>300%) amylase release, but starch has no effect. Proteins and amino acids have little or no effect on trypsin or aminopeptidase release. The control of enzyme release in response to food is likely mediated through neurohormones. Indeed, an allatostatin (Spofr-AS A5) inhibits amylase and trypsin, and allatotropin (Manse- AT) stimulates amylase and trypsin release. Spofr-AS A5 also inhibits ileum myoactivity and Manse-AT stimulates myoactivity. The epithelial secretion rate of amylase and trypsin was about 20% of the amount of enzyme present in the ventricular lumen, which, considering the efficient counter-current recycling of enzymes, suggests that the secretion rate is adequate to replace egested enzymes.
Lwalaba
Digali
D
Department of Animal Ecology I, University of Bayreuth, Bayreuth, Germany.
Hoffmann
Klaus H
KH
Woodring
Joseph
J
D000626:Aminopeptidases / Q000378:metabolism*; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D004064:Digestive System / Q000201:enzymology*; D005502:Food; D007082:Ileum / Q000378:metabolism; D066298:In Vitro Techniques; D007301:Insect Hormones / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000033:anatomy & histology / Q000502:physiology*; D008049:Lipase / Q000378:metabolism*; D009130:Muscle, Smooth / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D018411:Spodoptera / Q000502:physiology*; D014357:Trypsin / Q000378:metabolism*
D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; C076523:allatostatin; C050669:allatotropin; D008049:Lipase; D000681:Amylases; D000626:Aminopeptidases; D014357:Trypsin