title: Yeast proteome map (last update).
abstract: The identification of proteins separated on 2-D gels is essential to exploit the full potential of 2-D gel electrophoresis for proteomic investigations. For this purpose we have undertaken the systematic identification of Saccharomyces cerevisiae proteins separated on 2-D gels. We report here the identification by mass spectrometry of 100 novel yeast protein spots that have so far not been tackled due to their scarcity on our standard 2-D gels. These identifications extend the number of protein spots identified on our yeast 2-D proteome map to 716. They correspond to 485 unique proteins. Among these, 154 were resolved into several isoforms. The present data set can now be expanded to report for the first time a map of 363 protein isoforms that significantly deepens our knowledge of the yeast proteome. The reference map and a list of all identified proteins can be accessed on the Yeast Protein Map server (www.ibgc.u-bordeaux2.fr/YPM).
authors.affiliation: IBGC, UMR CNRS 5095 Bordeaux, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Perrot
authors.forename: Suzette
authors.initials: S
authors.lastname: Moes
authors.forename: Aurélie
authors.initials: A
authors.lastname: Massoni
authors.forename: Paul
authors.initials: P
authors.lastname: Jenoe
authors.forename: Hélian
authors.initials: H
authors.lastname: Boucherie
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D020407:Internet; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis*
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins