CD26 expression in donor stem cell harvest and its correlation with engraftment in human haematopoietic stem cell transplantation: potential predictor of early engraftment.
BACKGROUND
The efficiency of haematopoietic stem and progenitor cells (HSPCs) is important when donor cell numbers are limiting. Stable white blood cell (WBC) and platelet engraftment is crucial for the outcome of haematopoietic stem cell transplantation (HSCT).


METHODS
This article evaluates CD26/dipeptidyl peptidase-IV expression on mobilised peripheral blood stem cell (PBSC) harvest of donors and its correlation with engraftment in HSCT. We have analysed CD26 expression on cells in various gates, that is, lymphocytes, monocytes, neutrophils and all populations using flow cytometry tool.


RESULTS
Ours is the first study on human mobilised PBSC harvest from cancer patients or allogeneic related donors (n = 28) to demonstrate that increased CD26 expression leads to early engraftment in transplanted cancer patients. Correlation of CD26 expression with WBC engraftment was statistically significant (lymphocyte gate: P < 0.00001; monocyte gate: P < 0.00001; neutrophil gate: P < 0.00001; all populations: P < 0.00001). CD34 expression is a known predictor of engraftment. Nevertheless, there was no correlation between CD34 and CD26 expression in these cases.


CONCLUSIONS
This study has given important leads indicating that CD26 expression may be an independent predictor of engraftment. Further study with large number of patients as well as study on circulatory CD26 may add valuable information towards improving current knowledge on CD26.
Prabhash
K
K
Bone Marrow Transplantation Unit, Department of Medical Oncology.
Khattry
N
N
Bone Marrow Transplantation Unit, Department of Medical Oncology.
Bakshi
A
A
Bone Marrow Transplantation Unit, Department of Medical Oncology.
Karandikar
R
R
Chiplunkar Lab.
Joshi
A
A
Bone Marrow Transplantation Unit, Department of Medical Oncology.
Kannan
S
S
Epidemiology and Clinical Transfer Unit, Advanced Centre for Treatment, Research & Education in Cancer, Tata Memorial Centre, Navi Mumbai, India.
Sastry
P S R K
PSRK
Bone Marrow Transplantation Unit, Department of Medical Oncology.
Parikh
P
P
Bone Marrow Transplantation Unit, Department of Medical Oncology.
Kode
J A
JA
Chiplunkar Lab. Electronic address: jkode@actrec.gov.in.
D000293:Adolescent; D000328:Adult; D002648:Child; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000378:metabolism* / Q000628:therapy*; D015996:Survival Rate; D014019:Tissue Donors; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D055815:Young Adult
C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4