Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda.
In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.
Mills
Lisa A
LA
Division of Infectious Diseases, Johns Hopkins Medical Institutions, 1830 East Monument Street #401, Baltimore, MD 21287, USA. LisaAMills@Hotmail.com <LisaAMills@Hotmail.com>
Kagaayi
Joseph
J
Shott
Joseph P
JP
Newell
Kevin
K
Bwanika
John Baptist
JB
Ssempijja
Victor
V
Aluma
Simon
S
Quinn
Thomas C
TC
Reynolds
Steven J
SJ
Gray
Ronald H
RH
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003955:Diagnostic Tests, Routine / Q000379:methods / Q000592:standards*; D005260:Female; D006679:HIV Seropositivity; D006801:Humans; D016778:Malaria, Falciparum / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014454:Uganda; D055815:Young Adult