[Determination of six active components in three species of genus Swertia by HPLC multiwavelength with detection].
OBJECTIVE
To develop an HPLC method for the quantification of six active components in three species (Swertia davidi, S. nervosa and S. mussotii) .


METHODS
The determination was performed on a Hypersil BDS colunm (4. 6 mm x 200 mm, 5 microm). Acetonitrile and 0.5% phosphoric acid solution were used as the mobile phases with a gradient elution. The flow rate was 1.0 mL x min(-1). The UV detection wavelength was at 240, 274, 325 and 334 nm. The column oven temperature was at 25 degrees C.


RESULTS
Six components were separated commendably in 60 minutes. The calibration curves of swertiamarin, gentiopicroside, norswertianolin, swertianolin, demethylbellidifolin and bellidifolin were in good linearity over the range of 0.520-20.8, 0.202-8.06, 0.107-4.28, 0.097-3.86, 0.094-3.77, 0.101-4.02 microg, respectively (r = 0.999 9). The average recoveries were 98.7%, 98.1%, 98.3%, 98.8%, 98.1% and 98.6%, respectively, and the RSD were less than 3.0% (n = 6).


CONCLUSIONS
The method is accurate,simple and reproducible, and can be used to control the quality of Swertia.
Xu
Kangping
K
School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
Shen
Jian
J
Li
Fushuang
F
Liu
Jianfeng
J
Liu
Guoru
G
Tan
Jianbing
J
Tan
Guishan
G
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005960:Glucosides / Q000032:analysis; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis; D011753:Pyrones / Q000032:analysis; D029967:Swertia / Q000737:chemistry*; D044004:Xanthones / Q000032:analysis
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011753:Pyrones; D044004:Xanthones; C012997:gentiopicroside; C013270:swertiamarin; C056208:norswertianolin