New isolates of carnation Italian ringspot virus differ from the original one by having replication-associated proteins with a typical tombusvirus-like N-terminus and by inducing peroxisome- rather than mitochondrion-derived multivesicular bodies.
Five new isolates of carnation Italian ringspot virus (CIRV) from cherry trees, Gypsophila and surface water differ from the original carnation isolate (CIRV-car) and also from Pelargonium necrotic spot virus (PelNSV) by having an ORF 1/ORF1-RT with a typical tombusvirus-like 5'end and by inducing the formation of peroxisome- rather than mitochondrion-derived multivesicular bodies (MVBs). This supports with natural isolates earlier conclusions reached by others with artificially produced hybrid viruses that the 5'end of ORF 1 determines from which organelle the MBVs will be derived. CIRV-car might have resulted from a natural recombination event with genome elements of a PelNSV-like virus.
Koenig
Renate
R
c/o Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Braunschweig, Germany. renate.koenig@jki.bund.de
Lesemann
Dietrich-Eckhardt
DE
Pfeilstetter
Ernst
E
D029749:Dianthus / Q000821:virology; D016679:Genome, Viral / Q000235:genetics; D008928:Mitochondria / Q000821:virology; D008969:Molecular Sequence Data; D057146:Multivesicular Bodies / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D020675:Peroxisomes / Q000821:virology*; D027861:Prunus / Q000821:virology; D013728:Terminator Regions, Genetic / Q000235:genetics; D017864:Tombusvirus / Q000235:genetics* / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
D015534:Trans-Activators