[Study on effects of curcumin on expressions of PDGF-BB, PDGFRbeta and ERK1 of HSC].
OBJECTIVE
To observe the effects of curcumin on expression of PDGF-BB, PDGFRbeta and ERK1 of rat Hepatic stellate cell (HSC-T6).


METHODS
The cultured HSC were divided into control group, each curcumin treating group. The influence of curcumin on HSC proliferation was detected by MTT assay. The protein levels of PDGF-BB, PDGFRbeta and ERK1 was detected by immunhistochemical examination; The influence of curcumin on expression of PDGF-BB, PDGFRbeta and ERK1 mRNA was detected by RT-PCR.


RESULTS
Immunohistiochemical results showed that normal cultured HSC-T6 cell expressed PDGF-BB, PDGFRbeta and ERK1 remarkably. After the intervention of different concentration of curcumin,the masculine degrees of PDGF-BB, PDGFRbeta and ERK1 and the masculine cell population decreased obviously. The RT-PCR results indicated that the expressions of PDGF-BB, PDGFRbeta and ERK1 mRNA could be obviously seen in control and TGF-beta1 stimulus group. After the intervention of different concentration of curcumin, the expression of PDGF-BB, PDGFRbeta and ERK1 mRNA decreased in a dose-dependent manner.


CONCLUSIONS
Curcumin could inhibit the expression of PDGF-BB, PDGFRbeta and ERK1 which might be the mechanism of action curcumin on anti-fibrosis.
Zhao
Zhen-Dong
ZD
Guang Dong Food and Drug Vocational College, Guangzhou 510520, China. Zhaozd2008@126.com
Huang
Zhao-Sheng
ZS
D000818:Animals; D000077214:Becaplermin; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D004791:Enzyme Inhibitors; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D000077214:Becaplermin; D020797:Receptor, Platelet-Derived Growth Factor beta; D019950:Mitogen-Activated Protein Kinase 1; D003474:Curcumin