title: A determination of submicromolar concentrations of glycine in periaqueductal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection.
abstract: CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 microM by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
authors.affiliation: Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Czech Republic. petr.tuma@lf3.cuni.cz
authors.forename: Petr
authors.initials: P
authors.lastname: Tůma
authors.forename: Marie
authors.initials: M
authors.lastname: Soukupová
authors.forename: Eva
authors.initials: E
authors.lastname: Samcová
authors.forename: Karel
authors.initials: K
authors.lastname: Stulík
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D005998:Glycine / Q000032:analysis* / Q000378:metabolism; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D010487:Periaqueductal Gray / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen; D002351:Carrageenan; D005998:Glycine