title: Directed evolution of orthogonal ligand specificity in a single scaffold.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McLachlan
authors.forename: Karuppiah
authors.initials: K
authors.lastname: Chockalingam
authors.forename: Ka Chun
authors.initials: KC
authors.lastname: Lai
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D019020:Directed Molecular Evolution / Q000379:methods*; D004958:Estradiol / Q000737:chemistry / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008024:Ligands; D016296:Mutagenesis; D010658:Phenylglyoxal / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D017434:Protein Structure, Tertiary / Q000235:genetics; D013844:Thiazoles / Q000737:chemistry / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
chemical_list: C545673:2,4-di(4-hydroxyphenyl)-5-ethylthiazole; C545672:4,4'-dihydroxybenzil; D047628:Estrogen Receptor alpha; D008024:Ligands; D013844:Thiazoles; D004958:Estradiol; D010658:Phenylglyoxal