[An in vitro evaluation of the antibacterial activity of chitosan-based thermosensitive hydrogel against periodontal pathogens].
OBJECTIVE
To evaluate the in vitro antibacterial activity of chitosan - quaternized chitosan/alpha, beta-glycerophosphate (CS-HTCC/GP) thermosensitive hydrogel against three periodontal pathogens- P. gingivalis, P. intermedia, and A. actinomycetemcomitans.


METHODS
An agar diffusion method was used to assess the antimicrobial property of CS-HTCC/GP thermosensitive hydrogel with minimum inhibitory concentration (MIC) and inhibitory zone measurement. SPSS13.0 software package was used for Student's t test.


RESULTS
Three periodontal pathogens strains were all susceptible to CS-HTCC/GP thermosensitive hydrogel. Both matrix of thermosensitive hydrogel and antibiotic exhibited stronger antibacterial activity especially when they were combined.


CONCLUSIONS
CS-HTCC/GP thermosensitive hydrogel is not only as the vehicle of antibiotics which joins the local drug delivery system but as an activator which takes part in the antibacterial process.
Ji
Qiu-xia
QX
Department of Stomatology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China. jqx_1@163.com
Deng
Jing
J
Yu
Xin-bo
XB
Xu
Quan-chen
QC
Xu
Xiao-yan
XY
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D048271:Chitosan; D016503:Drug Delivery Systems; D005994:Glycerophosphates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D013696:Temperature
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D005994:Glycerophosphates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; C031463:beta-glycerophosphoric acid