title: Iron deficiency and frequency of HFE C282Y gene mutation in Brazilian blood donors.
abstract: Limited data are available about iron deficiency (ID) in Brazilian blood donors. This study evaluated the frequencies of ID and iron-deficiency anaemia (IDA) separately and according to frequency of blood donations. The protective effect of the heterozygous genotype for HFE C282Y mutation against ID and IDA in female blood donors was also determined. Five hundred and eight blood donors were recruited at the Blood Bank of Santa Casa in Sao Paulo, Brazil. Haemoglobin and serum ferritin concentrations were measured. The genotype for HFE C282Y mutation was determined by polymerase chain reaction followed by restriction fragment length polymorphism analysis. The ID was found in 21.1% of the women and 2.6% of the men whereas the IDA was found in 6.8 and 0.3%, respectively. The ID was found in 11.9% of the women in group 1 (first-time blood donors) and the frequency increased to 38.9% in women of the group 3 (blood donors donating once or more times in the last 12 months). No ID was found in men from group 1; however the ID frequency increased to 0.9% in group 2 (who had donated blood before but not in the last 12 months) and 5.0% in group 3. In summary, the heterozygous genotype was not associated with reduction of ID or IDA frequencies in both genders, but in male blood donors it was associated with a trend to elevated ferritin levels (P = 0.060). ID is most frequent in Brazilian women but was also found in men of group 3.
authors.affiliation: Department of Clinical Chemistry and Toxicology, School of Pharmaceutical Sciences, University of Sao Paulo, CEP 05508-900, SP, Brazil.
authors.forename: C T
authors.initials: CT
authors.lastname: Terada
authors.forename: P C J L
authors.initials: PC
authors.lastname: Santos
authors.forename: R D
authors.initials: RD
authors.lastname: Cançado
authors.forename: S
authors.initials: S
authors.lastname: Rostelato
authors.forename: F R
authors.initials: FR
authors.lastname: Lopreato
authors.forename: C S
authors.initials: CS
authors.lastname: Chiattone
authors.forename: E M
authors.initials: EM
authors.lastname: Guerra-Shinohara
mesh_terms: D000328:Adult; D017677:Age Distribution; D018798:Anemia, Iron-Deficiency / Q000453:epidemiology / Q000235:genetics*; D001782:Blood Donors / Q000706:statistics & numerical data*; D001938:Brazil; D005260:Female; D005293:Ferritins / Q000097:blood; D005820:Genetic Testing / Q000706:statistics & numerical data; D005838:Genotype; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D000090463:Iron Deficiencies; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D017354:Point Mutation; D012737:Sex Factors; D055815:Young Adult
chemical_list: C103673:HFE protein, human; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D005293:Ferritins