title: Structure of a novel highly branched alpha-glucan enzymatically produced from maltodextrin.
abstract: The bacterial strain PP710, isolated from soil and identified as Paenibacillus species, produced a low-digestibility alpha-glucan containing a large amylase-resistant portion. This alpha-glucan was obtained in high yields from maltodextrin (dextrose equivalent 3) by using the condensed culture supernatant of the strain as the enzyme preparation. The water-soluble dietary fiber content of the low-digestibility alpha-glucan was 80.2%, and showed resistance to a rat intestinal enzyme preparation. The alpha-glucan was found to be a novel highly branched alpha-glucan by acid hydrolysis, NMR analysis, gel permeation chromatography, methylation analysis, and enzymatic digestion.
authors.affiliation: Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc., Naka-ku, Okayama 702-8006, Japan. tsusaki@hayashibara.co.jp
authors.forename: Keiji
authors.initials: K
authors.lastname: Tsusaki
authors.forename: Hikaru
authors.initials: H
authors.lastname: Watanabe
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nishimoto
authors.forename: Takuo
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Michio
authors.initials: M
authors.lastname: Kubota
authors.forename: Hiroto
authors.initials: H
authors.lastname: Chaen
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D003910:Dextranase / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D001614:beta-Amylase / Q000378:metabolism
chemical_list: D004798:Enzymes; D005936:Glucans; D005960:Glucosides; D011134:Polysaccharides; C008315:maltodextrin; D006026:Glycoside Hydrolases; D003910:Dextranase; D001614:beta-Amylase; C091051:glucan 1,6-alpha-isomaltosidase