Evaluation of mobility of 2-N-tert-butylaminoxyladenosine incorporated into oligodeoxynucleotides.
We synthesized oligodeoxynucleotide (ODN, 3) containing 2-N-tert-butylaminoxyladenosine (1) and studied EPR spectra of 3 and its duplexes. The h(+)/h(0) values in the EPR spectra of duplexes between 3 and 5-8 well correlated with Tm values. We also synthesized ODN 4 containing 2, which has a cyclic aminoxyl via a linker, to compare with ODN 3. The h(+)/h(0) values in the EPR spectra of duplexes between 4 and 5-8 did not correlate with Tm values. These results indicate that 1 has a potential to monitor of motion of the nucleobase.
Higuchi
Yoshitaka
Y
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Aso
Mariko
M
Harada
Ryouko
R
Koga
Noboru
N
Suemune
Hiroshi
H
D000241:Adenosine / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D009038:Motion; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013113:Spin Labels
D009838:Oligodeoxyribonucleotides; D013113:Spin Labels; D000241:Adenosine