Generation of a human immunodeficiency virus type 1 chronically infected monkey B cell line expressing low levels of endogenous TRIM5alpha.
Several innate cellular antiviral factors exist in mammalian cells that prevent the replication of retroviruses. Among them, the tripartite motif protein (TRIM)5alpha has been shown to block human immunodeficiency virus type 1 (HIV-1) infection in several types of Old World monkey cells. Here we report a novel HIV-1 chronically infected monkey B cell line, F6/HIV-1, characterized by very low levels of TRIM5alpha expression that allows HIV-1 to overcome the restriction. Virus produced by F6/HIV-1 cells fails to infect monkey cells but retains the ability to infect human peripheral blood mononuclear cells (PBMCs) and T cell lines, although with a reduced infectivity compared to the input virus. Ultrastructural analyses revealed the presence of budding virions at the F6/HIV-1 cells plasma membrane characterized by a typical conical core shell. To our knowledge F6/HIV-1 is the first monkey cell line chronically infected by HIV-1 and able to release infectious particles thus representing a useful tool to gain further insights into the molecular mechanisms of HIV-1 pathogenesis.
Ridolfi
Barbara
B
National AIDS Center, Istituto Superiore di Sanità, Rome, Italy.
Catone
Stefania
S
Sgarbanti
Marco
M
Sernicola
Leonardo
L
Battistini
Angela
A
Parolin
Cristina
C
Titti
Fausto
F
Borsetti
Alessandra
A
D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D000090103:Antiviral Restriction Factors; D001402:B-Lymphocytes / Q000378:metabolism* / Q000821:virology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000235:genetics; D016655:HIV Core Protein p24 / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008252:Macaca fascicularis; D013045:Species Specificity; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D014771:Virion / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D014779:Virus Replication / Q000502:physiology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
D000090103:Antiviral Restriction Factors; D002352:Carrier Proteins; D016655:HIV Core Protein p24; D054298:Human Immunodeficiency Virus Proteins; D000070584:Tripartite Motif Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C477346:TRIM5 protein, human; D044767:Ubiquitin-Protein Ligases