title: Dynamic light-scattering measurement of sieving polymer solutions for protein separation on SDS CE.
abstract: We evaluated the mesh size and homogeneity of polymer network by dynamic light scattering and discussed the relationship between the physical properties of polymer network and the protein separation behavior by capillary polymer electrophoresis. We compared three kinds of sieving polymers in solutions with a wide range of molecular weights and concentrations: polyacrylamide and polyethylene oxide as flexible polymers, and hydroxyethyl cellulose as a semiflexible polymer. We found that the mobility of protein was dominated primarily by the mesh size xi, irrespective of the type of sieving polymers, and the peak spacing between protein peaks increased drastically in the range of xi<10 nm, where the mobility also decreased. And the peak widths were dependent on the molecular species of sieving polymers and their homogeneity of polymer network. We proposed that a polymer network with a homogenous mesh size of less than 10 nm is the best sieving medium for separation of the proteins in the molecular weight range 14,300-97,200 Da from the view point of the resolution in protein separation.
authors.affiliation: SORST, Japan Science and Technology Agency, Ibaraki, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Sumitomo
authors.forename: Koichi
authors.initials: K
authors.lastname: Mayumi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sakai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Minamikawa
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Masuda
authors.forename: Toshimi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kohzo
authors.initials: K
authors.lastname: Ito
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yamaguchi
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D008027:Light; D011506:Proteins / Q000302:isolation & purification*; D012542:Scattering, Radiation; D012967:Sodium Dodecyl Sulfate
chemical_list: D011506:Proteins; D012967:Sodium Dodecyl Sulfate