Evaluation of antimicrobial peptides as novel bactericidal agents for room temperature-stored platelets.
BACKGROUND
A single cost-effective pathogen inactivation approach would help to improve the safety of our nation's blood supply. Several methods and technologies are currently being studied to help reduce bacterial contamination of blood components. There is clearly need for simple and easy-to-use pathogen inactivation techniques specific to plasma, platelets (PLTs), and red blood cells.


METHODS
In this report, we introduce a novel proof of concept: using known therapeutic antimicrobial peptides (AMPs) as bactericidal agents for room temperature-stored PLT concentrates (PCs). Nine synthetic AMPs, four from PLT microbicidal protein-derived peptides (PD1-4) and five Arg-Trp (RW) repeat peptides containing one to five repeats, were tested for bactericidal activity in plasma and PC samples spiked with Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Bacillus cereus. A 3-log reduction of viable bacteria was considered as the bactericidal activity of a given peptide.


RESULTS
In both plasma alone and PCs, RW3 peptide demonstrated bactericidal activity against S. aureus, S. epidermidis, E. coli, P. aeruginosa, and K. pneumoniae; PD4 and RW2 against P. aeruginosa; and RW4 against K. pneumoniae. The activity of each of these four peptides against the remaining bacterial species in the test panel resulted in less than a 3-log reduction in the number of viable bacteria and hence considered ineffective.


CONCLUSIONS
These findings suggest a new approach to improving the safety of blood components, demonstrating the potential usefulness of screening therapeutic AMPs against selected bacteria to identify suitable bactericidal agents for stored plasma, PCs, and other blood products.
Mohan
Ketha V K
KV
Section of Cell Biology, Laboratory of Cellular Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
Rao
Shilpakala Sainath
SS
Atreya
Chintamani D
CD
D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001424:Bacterial Infections / Q000097:blood / Q000517:prevention & control* / Q000635:transmission; D000094902:Blood Banking / Q000379:methods*; D001792:Blood Platelets / Q000382:microbiology*; D001793:Blood Preservation; D006801:Humans; D008828:Microbiological Techniques; D010949:Plasma / Q000382:microbiology; D017713:Platelet Transfusion; D013696:Temperature
D023181:Antimicrobial Cationic Peptides