Determination of imatinib plasma levels in patients with chronic myeloid leukemia by high performance liquid chromatography-ultraviolet detection and liquid chromatography-tandem mass spectrometry: methods' comparison.
The aims of this study were to validate and compare HPLC and LCMSMS analytical methods and their applicability for the quantitation of imatinib in human plasma. A total of 50 patients with chronic myeloid leukemia (CML) in chronic phase (CP) receiving 400 mg/day imatinib were enrolled in the study. Drug levels were determined by HPLC-UV and LCMSMS. HPLC intra-day accuracy ranged from 100.51 to 103.19%. LCMSMS accuracy ranged from 89.72 to 106.29%. The correlation coefficient between both methods was r(2)=0.96. HPLC can be used for imatinib levels' determinations in patients accurately and precisely.
Awidi
Abdalla
A
Department of Medicine, Jordan University, Amman, Jordan. aawidi@yahoo.com
Salem
Isam I
II
Najib
Naji
N
Mefleh
Razan
R
Tarawneh
Bothainah
B
D000328:Adult; D000970:Antineoplastic Agents / Q000032:analysis / Q000097:blood / Q000627:therapeutic use; D001549:Benzamides; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D002940:Circadian Rhythm / Q000502:physiology; D005260:Female; D006801:Humans; D000068877:Imatinib Mesylate; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000188:drug therapy; D008297:Male; D008875:Middle Aged; D010879:Piperazines / Q000032:analysis* / Q000097:blood / Q000627:therapeutic use; D047428:Protein Kinase Inhibitors / Q000032:analysis / Q000097:blood / Q000627:therapeutic use; D011743:Pyrimidines / Q000032:analysis* / Q000097:blood / Q000627:therapeutic use; D016032:Randomized Controlled Trials as Topic; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; D000068877:Imatinib Mesylate