title: Multidrug-resistant variants of HIV type 1 (HIV-1) can exist in cells as defective quasispecies and be rescued by superinfection with other defective HIV-1 variants.
abstract: A tissue culture cell line infected with multidrug-resistant (MDR) human immunodeficiency virus type 1 (HIV-1) produced only noninfectious particles because of a lethal mutation in env. The defective MDR provirus was rescued by superinfection with either wild-type HIV-1 or a second replication-defective virus lethally mutated in capsid. Drug-resistance phenotyping revealed that the MDR viruses dominated if even single reverse-transcriptase inhibitors were present, reflecting linkage of the various drug resistance mutations on a single viral nucleic acid backbone. These results are most likely attributable to recombination during second rounds of infection and suggest that defective HIV-1 variants may nonetheless constitute part of the HIV-1 reservoir.
authors.affiliation: McGill AIDS Centre-Jewish General Hospital, Quebec, Canada.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Quan
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.forename: Bluma G
authors.initials: BG
authors.lastname: Brenner
authors.forename: Mark A
authors.initials: MA
authors.lastname: Wainberg
mesh_terms: D002460:Cell Line; D003673:Defective Viruses / Q000235:genetics*; D024921:Drug Resistance, Multiple, Viral / Q000235:genetics*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D018894:Reverse Transcriptase Inhibitors; D015163:Superinfection
chemical_list: D018894:Reverse Transcriptase Inhibitors; D012194:RNA-Directed DNA Polymerase