Subtractive hybridization identifies genes differentially expressed by olfactory ensheathing cells and neural stem cells.
The in vitro differentiation of embryonic stem cells into glia has received relatively limited attention to date when compared with the interest in the generation of neurons. We are interested in a particular glial phenotype, the aldynoglia, and their differentiation from multipotential neural precursors (MNP), since this type of glia can promote neuronal regeneration. We constructed cDNA libraries from cultures of purified olfactory ensheathing cells (OEC), an aldynoglia cell type, and MNP to perform subtractive hybridization. As a result, we isolated four genes from the OEC: one tenascin C (Tn-C) isoform, Insulin-like growth factor binding protein 5 (Igfbp-5), cytochrome oxidase subunit I (COX1) and a phosphodiesterase for cyclic nucleotides (CNPase). With the exception of CNPase, these genes are expressed more strongly in the OEC than in the MNP and moreover, the expression of all four is induced when MNP were exposed to OEC conditioned media. The data suggest a role for these genes in MNP differentiation, and their products appear to represent characteristic proteins of the aldynoglia phenotype.
Rojas-Mayorquín
Argelia Esperanza
AE
Laboratorio de Desarrollo y Regeneración Neural, Instituto de Neurobiología, Departamento de Biología Celular y Molecular, C.U.C.B.A, Universidad de Guadalajara, 45020 Guadalajara, Jalisco, México.
Torres-Ruíz
Nadia Magali
NM
Gudiño-Cabrera
Graciela
G
Ortuño-Sahagún
Daniel
D
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D005821:Genetic Techniques; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*; D019063:Tenascin / Q000235:genetics / Q000378:metabolism
D017077:Culture Media, Conditioned; D005904:Glial Fibrillary Acidic Protein; D018975:Insulin-Like Growth Factor Binding Protein 5; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D019063:Tenascin; C068313:Ngfr protein, rat; D051545:Cyclooxygenase 1; C496513:Ptgs1 protein, rat; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases