title: A new Neotyphodium species from Festuca parvigluma Steud. grown in China.
abstract: Neotyphodium species evolved from Epichloë species and are asexual, seedborne endophytes in many cool-season grasses. Here we propose a new species inhabiting Festuca parvigluma native to China. Morphology, host specificity and molecular phylogenetic evidence supported recognition of this new species. Sequences of beta-tubulin gene (tubB) introns and translation elongation factor 1-alpha gene (tefA) introns were present as two copies in all five isolates examined. In phylogenetic analyses copy 1 was closely related to E. yangzii in the EBY clade and copy 2 with E. typhina in the ETC clade, indicating this new species might have originated as a result of hybridization between members of these two clades. Referring to the distribution area of host plants, Neotyphodium sinofestucae is proposed for this new species.
authors.affiliation: Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yong-gan
authors.initials: YG
authors.lastname: Chen
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Ji
authors.forename: Han-shou
authors.initials: HS
authors.lastname: Yu
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Wang
mesh_terms: D002681:China; D055329:Epichloe / Q000235:genetics; D031751:Festuca / Q000382:microbiology*; D006824:Hybridization, Genetic; D007438:Introns / Q000235:genetics; D055328:Neotyphodium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014404:Tubulin / Q000235:genetics
chemical_list: D020648:Peptide Elongation Factor 1; D014404:Tubulin