[Effects of ERK and STAT3 on cell signal transduction in alveolar epithelium A549 cells stimulated by mechanical force].
OBJECTIVE
To observe the effect of mechanical force on the expression of p-ERK and p-STAT3 when pulmonary epithelium A549 cells were subjected to tensile stress.


METHODS
By the self-made four-point bending system, the A549 cells were subjected to cyclic tensile stress of 3000 microstrain for 30 min, 1 h, 2 h or 4 h respectively, then the total protein of each time point was collected and the expression of p-ERK and p-STAT3 were detected by Western blot analysis.


RESULTS
(1) The expression of p-ERK was activated at 1 h and remained at a high level until 4 h. (2) The expression of p-STAT3 (Ser) was activated at 30 min and remained at a high level until 4 h.


CONCLUSIONS
Tensile stress can activate the expression of p-ERK and p-STAT3 (Ser) in alveolar epithelium A549 cells. ERK and STAT3 may participate in the cell mechanotransduction in A549 cells.
An
Jing
J
Department of Respiratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Han
Su-Xia
SX
Hou
Yan
Y
Zhou
Hong
H
Wang
Tao
T
Xu
Dan
D
Hu
Xiao-Rong
XR
Wen
Fu-Qiang
FQ
D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D011650:Pulmonary Alveoli / Q000166:cytology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D013314:Stress, Mechanical
D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D048049:Extracellular Signal-Regulated MAP Kinases