Verrucosispora sediminis sp. nov., a cyclodipeptide-producing actinomycete from deep-sea sediment.
An actinomycete, designated MS426T, the culture broth of which showed potent antimicrobial activity, was isolated from a deep-sea sediment sample of the South China Sea. An almost-complete sequence of the 16S rRNA gene of strain MS426T was determined and aligned with those of representatives of the family Micromonosporaceae available in public databases. Phylogenetic trees were inferred by using three algorithms. Strain MS426T formed a branch adjacent to Verrucosispora lutea YIM 013T in a distinct cluster occupied only by strains of the genus Verrucosispora. Strain MS426T was distinguishable from the type strains of the two described Verrucosispora species by using a combination of chemical and morphological markers and by DNA-DNA relatedness. On the basis of these genotypic and phenotypic differences, the novel antimicrobial strain with pharmaceutical potential represents a novel species, for which the name Verrucosispora sediminis sp. nov. is proposed. The type strain is MS426T (=CGMCC 4.3550T =JCM 15670T).
Dai
Huan-Qin
HQ
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Wang
Jian
J
Graduate University, CAS, Beijing 100049, PR China.|South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.|Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Xin
Yu-Hua
YH
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Pei
Gang
G
Graduate University, CAS, Beijing 100049, PR China.|Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Tang
Shu-Kun
SK
The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Ren
Biao
B
Graduate University, CAS, Beijing 100049, PR China.|Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Ward
Alan
A
Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
Ruan
Ji-Sheng
JS
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.|South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Zhang
Li-Xin
LX
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.|Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004151:Dipeptides / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004151:Dipeptides; D012336:RNA, Ribosomal, 16S