title: A 5' leader sequence regulates expression of methanosarcinal CO dehydrogenase/acetyl coenzyme A synthase.
abstract: In vivo expression of CO dehydrogenase/acetyl coenzyme A synthase in Methanosarcina spp. is coordinately regulated in response to substrate by at least two mechanisms: differential transcription initiation and early elongation termination near the 3' end of a 371-bp leader sequence. This is the first report of regulation of transcription elongation in the Archaea.
authors.affiliation: Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt St., Baltimore, MD 21202, USA.
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: Anderson
authors.forename: Ethel E
authors.initials: EE
authors.lastname: Apolinario
authors.forename: Sheridan R
authors.initials: SR
authors.lastname: MacAuley
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Sowers
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics / Q000502:physiology; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000502:physiology*; D019843:Archaeal Proteins / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D019848:Gene Expression Regulation, Archaeal / Q000235:genetics; D017020:Methanosarcina / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D019843:Archaeal Proteins; D009097:Multienzyme Complexes; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase