Biochemical dissection of RISC assembly and function.
Small silencing RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs), regulate expression of their target genes via RNA-induced silencing complex (RISC). RISC assembly follows complex, ordered pathways, and RISC function is as diverse as cleavage, translational repression, and deadenylation of the target. We have recently shown how siRNAs and miRNAs are assembled into distinct types of RISC, and how differently they function, using Drosophila as a model organism.
Tomari
Yukihide
Y
Department of Medical Genome Sciences, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. tomari@iam.u-tokyo.ac.jp
D000818:Animals; D060565:Argonaute Proteins; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D039642:Eukaryotic Initiation Factors / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D014176:Protein Biosynthesis; D034743:RNA-Induced Silencing Complex / Q000378:metabolism*
C489654:AGO1 protein, Drosophila; D060565:Argonaute Proteins; D029721:Drosophila Proteins; D039642:Eukaryotic Initiation Factors; D035683:MicroRNAs; D034743:RNA-Induced Silencing Complex