Identification of heavy metal regulated genes from the root associated ascomycete Cadophora finlandica using a genomic microarray.
The ascomycete Cadophora finlandica, which can form mycorrhizas with ectomycorrhizal and ericoid hosts, is commonly found in heavy metal polluted soils. To understand the selective advantage of this organism at contaminated sites heavy metal regulated genes from C. finlandica were investigated. For gene identification a strategy based on a genomic microarray was chosen, which allows a rapid, genome-wide screening in genetically poorly characterized organisms. In a preliminary screen eleven plasmids covering eight distinct genomic regions and encoding a total of ten Cd-regulated genes were identified. Northern analyses with RNA from C. finlandica grown in the presence of either Cd, Pb or Zn revealed different transcription patterns in response to the heavy metals present in the growth medium. The Cd-regulated genes are predicted to encode several extracellular proteins with unknown functions, transporters, a centaurin-type regulator of intracellular membrane trafficking, a GNAT-family acetyltransferase and a B-type cyclin.
Gorfer
Markus
M
Department of Applied Genetics and Cell Biology, Fungal Genomics Unit, Austrian Research Centers and BOKU-University Vienna, Muthgasse 18, 1190 Vienna, Austria. markus.gorfer@boku.ac.at
Persak
Helene
H
Berger
Harald
H
Brynda
Sabine
S
Bandian
Dragana
D
Strauss
Joseph
J
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000327:Adsorption; D001203:Ascomycota / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000494:pharmacology; D019295:Computational Biology; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D007220:Industrial Waste; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D019216:Metals, Heavy / Q000494:pharmacology*; D005810:Multigene Family; D038821:Mycorrhizae / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018517:Plant Roots / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000494:pharmacology*
D007220:Industrial Waste; D026901:Membrane Transport Proteins; D019216:Metals, Heavy; D012989:Soil Pollutants; D002104:Cadmium; D000251:Adenosine Triphosphatases