Oxidative burst inhibitory and cytotoxic amides and lignans from the stem bark of Fagara heitzii (Rutaceae).
Two amides, heitziamide A and heitziamide B and two phenylethanoids, heitziethanoid A and heitziethanoid B together with thirteen known compounds were isolated from F. heitzii (Letouzey). The structures of all compounds were established by spectroscopic analysis. Nine compounds were evaluated for oxidative burst inhibitory activity in a chemoluminescence assay and for cytotoxicity against PC-3 prostate cancer cells. All compounds exhibited a clear suppressive effect on phagocytosis response upon activation with serum opsonized zymosan at the range of IC(50)=2.0-6.5 microM, but no cytotoxic effect was observed (IC(50)>100 microM).
Mbaze
Luc Meva'a
LM
Department of Chemistry, Faculty of Science, University of Douala, P.O. Box 24157, Douala, Cameroon. lmbazze@yahoo.fr
Lado
Jean Alexandre
JA
Wansi
Jean Duplex
JD
Shiao
Tze Chieh
TC
Chiozem
David Dako
DD
Mesaik
Muhammad Ahmed
MA
Choudhary
Muhammad Iqbal
MI
Lacaille-Dubois
Marie-Aleth
MA
Wandji
Jean
J
Roy
René
R
Sewald
Norbert
N
D000577:Amides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D006574:Heterocyclic Compounds, 2-Ring / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D010587:Phagocytosis / Q000187:drug effects*; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018547:Plant Stems; D011471:Prostatic Neoplasms / Q000188:drug therapy; D016897:Respiratory Burst / Q000187:drug effects*; D027821:Rutaceae / Q000737:chemistry*; D015054:Zymosan / Q000097:blood
D000577:Amides; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D006574:Heterocyclic Compounds, 2-Ring; D007155:Immunologic Factors; D017705:Lignans; D010936:Plant Extracts; C545973:heitziamide A; C545974:heitziamide B; C545975:heitziethanoid A; C545976:heitziethanoid B; D015054:Zymosan