Expression profiles of seven glutathione S-transferase (GST) genes in cadmium-exposed river pufferfish (Takifugu obscurus).
Glutathione S-transferase (GST; EC 2.5.1.18) plays a critical role in detoxification pathways. In this study, we report cloning and expression of seven genes of the GST family of the pufferfish Takifugu obscurus together with mRNA tissue distribution pattern and time-course of expression in response to exposure to cadmium. At basal levels of tissue expression, GST-Mu is highly expressed in liver compared with other tissues. When fish were exposed to cadmium (5 mg/L for 96 h), expression of GST-MAPEG, GST-Mu, GST-Omega, and GST-Zeta was greatly increased, whereas GST-Alpha and GST-Kappa genes showed no significant response. These findings suggest that gene expression of a number of GST isoforms in T. obscurus is modulated in response to exposure to cadmium. We propose GST-Mu, GST-Theta, and GST-Zeta as candidate biomarkers for heavy metal exposure in this fish.
Kim
Jin-Hyoung
JH
National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea.
Dahms
Hans-Uwe
HU
Rhee
Jae-Sung
JS
Lee
Young-Mi
YM
Lee
Jehee
J
Han
Kyung-Nam
KN
Lee
Jae-Seong
JS
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003001:Cloning, Molecular; D004784:Environmental Monitoring; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005819:Genetic Markers; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D023741:Takifugu / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D029941:Fish Proteins; D005819:Genetic Markers; D007527:Isoenzymes; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D002104:Cadmium; D005982:Glutathione Transferase