title: Autoantibody screen in inflammatory myopathies high prevalence of antibodies to gliadin.
abstract: BACKGROUND
  Inflammatory myopathies (IM) are associated with autoimmune diseases.
  
  
  OBJECTIVE
  To evaluate the titers of auto-antibodies specific to various autoimmune diseases in patients with IM compared with controls.
  
  
  METHODS
  Sera from 99 IM patients and 100 healthy controls were tested for autoantibodies for vasculitis (myeloperoxidase, PR3, and glomerular basement membrane) and autoimmune gastrointestinal diseases (IgA and IgG antigliadin, antitissue transglutaminase, and Saccharomyces cerevisiae) utilizing the BioPlex 2200 Multiplexed Immunoassay method (Biorad).
  
  
  RESULTS
  Antigliadin IgA levels were significantly elevated in IM patients compared with controls (0.37 units +/- 0.44 vs. 0.24 units +/- 0.15, P = 0.017). Antitissue transglutaminase IgA was marginally increased in IM patients versus controls (0.36 units +/- 1.12 vs. 0.2 units +/- 0.0, P = 0.08).
  
  
  CONCLUSIONS
  Antibodies to gliadin and tissue transglutaminase characteristic for celiac disease were elevated in patients with IM compared with controls. This may indicate a higher prevalence of gluten sensitivity or celiac disease in IM.
authors.affiliation: Department of Medicine B, Wolfson Medical Center, Holon, Israel.
authors.forename: Hedi
authors.initials: H
authors.lastname: Orbach
authors.forename: Nimrod
authors.initials: N
authors.lastname: Amitai
authors.forename: Ori
authors.initials: O
authors.lastname: Barzilai
authors.forename: Mona
authors.initials: M
authors.lastname: Boaz
authors.forename: Maya
authors.initials: M
authors.lastname: Ram
authors.forename: Gisele
authors.initials: G
authors.lastname: Zandman-Goddard
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Shoenfeld
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D002446:Celiac Disease / Q000175:diagnosis; D003430:Cross-Sectional Studies; D005260:Female; D019204:GTP-Binding Proteins; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D009220:Myositis / Q000175:diagnosis*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D011503:Transglutaminases / Q000276:immunology; D014657:Vasculitis / Q000175:diagnosis
chemical_list: D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins