Gastric afferents project to the aldosterone-sensitive HSD2 neurons of the NTS.
The HSD2 (11-beta-hydroxysteroid dehydrogenase-type 2 enzyme) containing neurons of the nucleus tractus solitarius (NTS) become activated during low-sodium and high-aldosterone states such as hypovolemia. This response may be due to hormonal and/or neural signals. Hormonal signals may activate neurons in the area postrema that innervate the HSD2 neurons. The vagus nerve projects directly to the HSD2 neurons and this could be another route whereby these neurons receive information about systemic sodium/aldosterone status. The peripheral sites of origin that contribute to this vagal projection remain unknown, and in the present study, we injected the transganglionic tracer, cholera toxin beta-subunit-horseradish peroxidase (CTb-HRP), into wall of various gastrointestinal organs (stomach, small and large intestine) or liver of rats. Confocal microscopy of brainstem sections stained by a double immunohistochemical procedure was used to analyze whether the HSD2 neurons received axonal contacts from specific gastrointestinal structures. The major source of afferents arose from the stomach, mainly from its pyloric antrum, but a weaker input originated from the fundus region. A trace amount originated from the duodenum. The terminal part of the small intestine and large intestine did not to contribute to this projection. Similarly, no afferent inputs from the liver or portal vein were found. In conclusion, HSD2 neurons receive an input mainly from the stomach and these results are considered as potential sites affecting sodium intake.
Shin
Jung-Won
JW
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110, USA. jshin@pcg.wustl.edu
Loewy
Arthur D
AD
D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000378:metabolism*; D000344:Afferent Pathways / Q000378:metabolism / Q000502:physiology; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002452:Cell Count; D004386:Duodenum / Q000502:physiology; D005260:Female; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D008297:Male; D018613:Microscopy, Confocal; D056325:Neuronal Tract-Tracers; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012982:Sodium, Dietary / Q000378:metabolism; D017552:Solitary Nucleus / Q000378:metabolism / Q000502:physiology*; D013270:Stomach / Q000502:physiology*
D056325:Neuronal Tract-Tracers; D012982:Sodium, Dietary; D000450:Aldosterone; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2