Absence of the major light-harvesting antenna proteins alters the redox properties of photosystem II reaction centres in the chlorina F2 mutant of barley.
Although the chlorina F2 mutant of barley specifically exhibits reduced levels of the major light-harvesting polypeptides associated with photosystem II (PSII), thermoluminescence measurements of photosystem reaction centre photochemistry revealed that S2/S3QB- charge recombinations were shifted to lower temperatures, while the characteristic peak of S2QA- charge recombinations was shifted to higher temperatures compared with wild-type (WT) barley. Thus, we show that the absence of the major light-harvesting polypeptides affects the redox properties of PSII reaction centres. Radiolabeling studies in vivo and in vitro with [32P]orthophosphate or [gamma-32P]ATP, respectively, demonstrated that the D1 PSII reaction centre polypeptide is phosphorylated in both the WT and the F2 mutant. In contrast with the radiolabeling results, phosphorylation of D1 and other PSII proteins, although detected in WT barley, was ambiguous in the F2 mutant when the phosphothreonine antibody method of detection was used. Thus, caution must be exercised in the use of commercially available phosphothreonine antibodies to estimate thylakoid polypeptide phosphorylation. Furthermore, in membrano, the D1 polypeptide of the F2 mutant was less susceptible to trypsin treatment than that of WT barley. The role of the light-harvesting complex in modulating the structure and function of the D1 polypeptide of PSII reaction centers is discussed.
Krol
Marianna
M
Department of Biology and the Biotron Experimental Climate Change Research Centre, University of Western Ontario, 1151 Richmond Street N., London, ON N6A 5B7, Canada.
Ivanov
Alexander G
AG
Booij-James
Isabelle
I
Mattoo
Autar K
AK
Sane
P V
PV
Hüner
Norman P A
NP
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D001467:Hordeum / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D010766:Phosphorylation; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D020524:Thylakoids / Q000378:metabolism
D045332:Photosystem II Protein Complex