title: Two novel null alleles of the KEL gene detected in two Chinese women with the K(null) phenotype.
abstract: In screening 87665 unrelated healthy blood donors in China, serology studies resulted in the detection of two K(0) probands, both female. To explore the molecular basis of the K(null) phenotype in the Chinese population, genomic DNA, total RNA, and reticulocyte RNA were subsequently prepared from the two probands, five family members of proband 1, four unrelated normal controls, and one unrelated KEL1 control. Nucleic acids were analyzed for the KEL gene by DNA and RNA sequencing, while antigens were analyzed by flow cytometry with BRIC18, BRIC68, anti-k, and anti-Kp(b). Two novel K(null) alleles were identified in both probands: in exon 3, 185insT (Ser62Phe and a premature stop codon in exon 4, GenBank accession number, EF208900), and in exon 7, 715G>T (Glu239Stop, GenBank accession number EF208901). Alternative splicing patterns were observed in RNA obtained from whole blood versus from a reticulocyte fraction. Our study identified these two novel K(null) alleles resulting in the K(null) phenotype, the frequency of the K(null) phenotype amongst Chinese mainlanders is only 0.00228%.
authors.affiliation: Shanghai Blood Center, Shanghai, China 200051. yangyinghla@yahoo.com.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: LingLing
authors.initials: L
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: HePing
authors.initials: H
authors.lastname: Chen
authors.forename: ZhongHui
authors.initials: Z
authors.lastname: Guo
authors.forename: YuXian
authors.initials: Y
authors.lastname: Zhang
authors.forename: ZiYan
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D000483:Alleles; D017398:Alternative Splicing; D000941:Antigens / Q000032:analysis; D001483:Base Sequence; D002681:China; D005091:Exons; D005260:Female; D005787:Gene Frequency; D005820:Genetic Testing; D006801:Humans; D007626:Kell Blood-Group System / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA
chemical_list: D000941:Antigens; D007626:Kell Blood-Group System