title: A Wnt kinase network alters nuclear localization of TCF-1 in colon cancer.
abstract: Constitutive activation of the Wnt/beta-catenin pathway has been implicated as the primary cause of colon cancer. However, the major transducers of Wnt signaling in the intestine, T-cell factor 1 (TCF-1) and TCF-4, have opposing functions. Knockout of TCF-4 suppresses growth and maintenance of crypt stem cells, whereas knockout of TCF-1 leads to adenomas. These phenotypes suggest that TCF-4 is Wnt-promoting, whereas TCF-1 acts like a tumor suppressor. Our study of TCF expression in human colon crypts reveals a mechanistic basis for this paradox. In normal colon cells, a dominant-negative isoform of TCF-1 (dnTCF-1) is expressed that is equally distributed between nuclear and cytoplasmic compartments. In colon cancer cells, TCF-1 is predominantly cytoplasmic. Localization is because of active nuclear export and is directed by an autocrine-acting Wnt ligand that requires Ca2+/calmodulin-dependent kinase II (CaMKII) activity for secretion and a downstream step in the export pathway. TCF-4 remains nuclear; its unopposed activity is accompanied by downregulation of dnTCF-1 and increased expression of full-length isoforms. Thus, the dnTCF-1 and TCF-4 balance is corrupted in cancer by two mechanisms, a Wnt/CaMKII kinase signal for nuclear export and decreased dnTCF-1 expression. We propose that dnTCF-1 provides homeostatic regulation of Wnt signaling and growth in normal colon, and the alterations in nuclear export and promoter usage contribute to aberrant Wnt activity in colon cancer.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697, USA.
authors.forename: R
authors.initials: R
authors.lastname: Najdi
authors.forename: A
authors.initials: A
authors.lastname: Syed
authors.forename: L
authors.initials: L
authors.lastname: Arce
authors.forename: H
authors.initials: H
authors.lastname: Theisen
authors.forename: J-Ht
authors.initials: JH
authors.lastname: Ting
authors.forename: F
authors.initials: F
authors.lastname: Atcha
authors.forename: A V
authors.initials: AV
authors.lastname: Nguyen
authors.forename: M
authors.initials: M
authors.lastname: Martinez
authors.forename: R F
authors.initials: RF
authors.lastname: Holcombe
authors.forename: R A
authors.initials: RA
authors.lastname: Edwards
authors.forename: J L
authors.initials: JL
authors.lastname: Marsh
authors.forename: M L
authors.initials: ML
authors.lastname: Waterman
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D019169:Jurkat Cells; D015398:Signal Transduction / Q000235:genetics; D051822:T Cell Transcription Factor 1 / Q000235:genetics / Q000378:metabolism*; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D051822:T Cell Transcription Factor 1; C532096:TCF4 protein, human; C497576:TCF7 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D051153:Wnt Proteins; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2