title: ENU induced single mutation locus on chr 16 leads to high-frequency hearing loss in mice.
abstract: The hallmark of age-related (presbycusis) and noise-induced hearing loss is high-frequency (> 20 kHz) hearing loss. Through a collaborative study with TMGC (Tennessee Mouse Genome Consortium), seventeen ENU-induced mouse mutation strains with high-frequency hearing loss have been identified, but affected genes are yet identified. As a first step in identifying the gene/s underlying the ENU mutations, we created a F2 population between a representative mutation strain, 118 TNE and a wild type strain, CAST/EJ (CAST). Phenotypic analysis showed that there is a 3:1 ratio of segregation between normal and hearing loss in the F2 population, suggestion a single locus regulation. However, the linkage mapping identified 2 QTLs, each on chromosomes 15 and 16. Further statistical analysis of marker segregation patterns revealed that the locus on Chr 16 was ENU induced while the one on Chr 15 was derived from the parental strain, CAST.
authors.affiliation: Departments of Orthopaedic Surgery-Campbell Clinic and Pathology, University of Tennessee Health Science Center (UTHSC), Memphis, TN, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiao
authors.forename: Chun
authors.initials: C
authors.lastname: Cai
authors.forename: Mohammad Habiby
authors.initials: MH
authors.lastname: Kermany
authors.forename: Jian
authors.initials: J
authors.lastname: Yan
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.forename: Darla
authors.initials: D
authors.lastname: Miller
authors.forename: Daniel
authors.initials: D
authors.lastname: Goldowitz
authors.forename: Xinmin
authors.initials: X
authors.lastname: Li
authors.forename: Tai-June
authors.initials: TJ
authors.lastname: Yoo
authors.forename: Weikuan
authors.initials: W
authors.lastname: Gu
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000737:chemistry / Q000187:drug effects*; D003433:Crosses, Genetic; D005038:Ethylnitrosourea / Q000633:toxicity*; D005819:Genetic Markers / Q000502:physiology; D006316:Hearing Loss, High-Frequency / Q000139:chemically induced* / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009154:Mutation / Q000187:drug effects / Q000502:physiology*; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000187:drug effects
chemical_list: D005819:Genetic Markers; D005038:Ethylnitrosourea