title: [Construction of mammalian cell lines continuously expressing JEV structural proteins].
abstract: Based on the infectious clone of JEV vaccine strain SA14-14-2, prM-E genes and C-prM-E genes were cloned into pCDNA3.1 vector. The recombinant plasmid pCJE-ME was transfected into BHK-21 cells, the expressed proteins were toxic to the cell growth and accelerated the cell death. But when transfected with the plasmid pCJE-CME, the cell lines continuously expressing structural proteins could be selected with G418. And the expression products of pCJE-CME vector could be detected by ELISA, Western Blot and IFA assay. It showed that the JEV capsid protein could enhance the stability of the cell lines expressing the structural proteins. The established cell lines can make the acquirement of the virus-like particles much easier.
authors.affiliation: Lab for Protein Engineering, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Sun
authors.forename: Wei-Yuan
authors.initials: WY
authors.lastname: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis; D016466:CHO Cells; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line / Q000166:cytology / Q000821:virology*; D006224:Cricetinae; D003412:Cricetulus; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D015678:Viral Structural Proteins