Mapping 3-dimensional neovessel organization steps using micro-computed tomography in a murine model of hindlimb ischemia-brief report.
OBJECTIVE
Studying the mechanisms of neovascularization and evaluating the effects of proangiogenic strategies require accurate analysis of the neovascular network. We sought to evaluate the contribution of the microcomputed tomography (mCT) providing high-resolution 3-dimensional (3D) structural data, to a better comprehension of the well-studied mouse hindlimb postischemic neovascularization.


RESULTS
We showed a predominant arteriogenesis process in the thigh and a predominant angiogenesis-related process in the tibiofibular region, in response to ischemia during the first 15 days. After 15 days, mCT quantitative analysis reveals a remodeling of arterial neovessels and a regression depending on the restoration of the blood flow. We provided also new mCT data on the rapid and potent angiogenic effects of mesenchymal stem cell therapy on vessel formation and organization. We discussed the contribution of this technique compared with or in addition to data generated by the more conventional approaches.


CONCLUSIONS
This study demonstrated that optimized mCT is a robust method for providing new insights into the 3D understanding of postischemic vessel formation.
Oses
Pierre
P
Inserm U828, Pessac, Université Victor Ségalen, Bordeaux, France.
Renault
Marie-Ange
MA
Chauvel
Rémi
R
Leroux
Lionel
L
Allières
Cécile
C
Séguy
Benjamin
B
Lamazière
Jean-Marie Daniel
JM
Dufourcq
Pascale
P
Couffinhal
Thierry
T
Duplàa
Cécile
C
D000818:Animals; D001464:Barium; D003287:Contrast Media; D004195:Disease Models, Animal; D006614:Hindlimb / Q000098:blood supply*; D021621:Imaging, Three-Dimensional; D007511:Ischemia / Q000473:pathology* / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D009387:Neoprene; D018919:Neovascularization, Physiologic; D016491:Peripheral Vascular Diseases / Q000473:pathology / Q000601:surgery; D014057:Tomography, X-Ray Computed / Q000379:methods*
D003287:Contrast Media; D001464:Barium; D009387:Neoprene