Trace analysis of glyclazide in human plasma at microscale level by mass spectrometry.
Green (reagents and organic solvents saving) analytical chemistry is a new strategy for pharmaceutical analysis. The principles of this idea include primary elimination or at least reduction of the amounts of organic reagents and solvents. In this study, we have provided two simple methods for the analysis of clinical drugs in human plasma. One is the capillary LC (Cap LC) connected to MS-MS, the other is the matrix-assisted laser desorption ionization (MALDI) connected to TOF MS. Sulfonylurea drugs are usually used in diabetes mellitus patients. Diabetes is a syndrome of disordered metabolism resulting in abnormally high blood sugar levels (hyperglycemia). These microscale methods were successfully applied for the monitoring of drug levels in human plasma using gliclazide (a second-generation sulfonylurea) as the test platform. The sensitivity of these methods is sufficient for detecting the gliclazide within a therapeutic range. All the analytical procedures (including human plasma, sample preparation, and flow rate of the analytical system) were at microscale level. These two methods would lower the consumption of organic solvents further safeguarding our environment.
Feng
Chia-Hsien
CH
Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
Yang
Chuan-Ming
CM
Lu
Chi-Yu
CY
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005907:Gliclazide / Q000097:blood* / Q000737:chemistry; D055772:Green Chemistry Technology; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood* / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014044:Tolbutamide / Q000737:chemistry
D007004:Hypoglycemic Agents; D012997:Solvents; D014044:Tolbutamide; D005907:Gliclazide