Sequence analysis of an isolate of minute virus of canines in China reveals the closed association with bocavirus.
In the present study, we have cloned and sequenced the nearly-full-length genome of minute virus of canines (MVC), SH26, in China. The genome of MVC, 5,132 nucleotides (nts) in length, contains three open reading frames (ORFs), which are 2,325-bp of NS1, 561-bp of NP1 and 2,112-bp of VP1/VP2 encoding three proteins of 774, 186 and 703 residues, respectively. Predicted amino acids sequence of NS1 of MVC has 44% identity with human bocavirus (HBoV) and human boacvirus 2 (HBoV2), NP1 has 48 and 45% identity with HBoV and HBoV2, VP1/VP2 has 45 and 46% identity with HBoV and HBoV2, respectively. Phylogenetic analysis showed that the present Chinese MVC strain was also closely clustered with the previous American and Japanese MVC isolates, and MVCs formed a different branch together with bovine parvovirus and HBoVs from other parvoviruses classified into Parvovirinae.
Shan
T L
TL
Zoonosis and Comparative Medicine Group, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, People's Republic of China. shantongling@163.com
Cui
L
L
Dai
X Q
XQ
Guo
W
W
Shang
X G
XG
Yu
Y
Y
Zhang
W
W
Kang
Y J
YJ
Shen
Q
Q
Yang
Z B
ZB
Zhu
J G
JG
Hua
X G
XG
D000818:Animals; D052660:Bocavirus / Q000235:genetics* / Q000302:isolation & purification*; D002681:China; D004285:Dogs; D006801:Humans; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*