Morphometric analysis of AgNORs in the lymph node lesions.
Silver stained argyrophilic nucleolar organizer regions (AgNOR) had widely been used for diagnostic as well as prognostic purposes in surgical pathology. Our study was carried out for the quantitative and morphometric analysis of AgNORs in various lymph node lesions. Ten cases each of reactive lymphadenitis and non-Hodgkin's lymphoma (NHL) were included in the present study. Out of 10 cases of NHL, 5 cases each were of Low grade and High grade lymphomas. The morphometric analysis and quantification of AgNORs were curried out using a specific programme of an automatic image analysis Biovis Image measure version 1.0. The results revealed that mean AgNOR count was maximum in high grade lymphoma (5.17 +/- 0.255) when compared with low grade lymphoma (4.78 +/- 0.41) and reactive lymph nodes (2.45 +/- 0.486). Similarly, the mean of total AgNOR area in high grade lymphoma (511.9 +/- 41.52) was highest followed by low grade lymphoma (451.12 +/- 37.1) and reactive lymphadenitis (215 +/- 37). However, the mean of ratio of single AgNOR dot area to nuclear area was lowest in high grade lymphoma (0.0718 +/- 0.01) in comparison to low grade lymphoma (0.0826 +/- 0.0096) and reactive lymph node (0.132 +/- 0.01). Thus, it can be concluded from the present study that different features of AgNOR evaluated morphometrically (AgNOR count, total AgNOR area, AgNOR dot area, ratio of total AgNOR area and single AgNOR area to nuclear area) were significantly altered in various lymph node lesions which may be helpful in differential diagnosis of problematic cases.
Arora
B
B
Department of Pathology, P.G.I.M.S., Rohtak, India. drbarora@rediffmail.com
Jain
Rahul
R
Kumar
Sanjay
S
Rekhi
Bharat
B
Arora
Hina
H
Arora
D R
DR
D034961:Antigens, Nuclear / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008199:Lymphadenitis / Q000378:metabolism / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000378:metabolism / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000473:pathology*; D016622:Silver Staining
D034961:Antigens, Nuclear; C051963:nucleolar organizer region associated proteins