[Acetylation of hepatic histone H3 in rats with intrauterine growth retardation].
OBJECTIVE
To investigate the effects of intrauterine growth retardation (IUGR) caused by malnutrition during pregnancy on the acetylation of histone H3 and expression of histonedeacetylase1(HDAC1) in the hepar of the adult offspring and to explore the relationship between them.


METHODS
Male 8-week-old offspring from maternal protein-malnutrition dams were studied. The expression of HDAC1 mRNA in the hepar was measured by fluorescent quantization RT-PCR. The levels of hepatic nuclear HDAC1 protein and acetylation of histone H3/K9 were assessed by Western blot.


RESULTS
The hepatic HDAC1 mRNA expression in IUGR rats was reduced to 54% of that of normal control rats (t=2.042, p<0.05). A decline in nuclear expression of HDAC1 protein (438 +/- 47) was also noted when compared with normal controls (1,128 +/- 110) (t=2.179, p<0.05). In contrast, the percentage of acetylated histone H3/K9 in IUGR rats (17.3 +/- 1.6%) increased significantly compared with that of normal control rats (10.5 +/- 1.2%) (t=3.597, p<0.01). The level of acetylated histone H3/K9 was negatively correlated with the HDAC1 protein concentration (r=-0.781, p<0.01).


CONCLUSIONS
Increased hepatic acetylation of histone H3 in the IUGR offspring might be caused by decreased HDAC1 expression in nuclear protein. This may contribute to the transcription change of some genes in the hepar.
Liu
Xiao-Mei
XM
Experimental Research Center, Shengjing Hospital of China Medical University, Shenyang 110004, China. liuxm1@sj-hospital.org
Zhang
Hai-Lan
HL
Song
Wei-Wei
WW
Jiao
Yi-Sheng
YS
Lu
Yan
Y
D000107:Acetylation; D000818:Animals; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D056284:Histone Deacetylase 1 / Q000032:analysis / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D006657:Histones; D012333:RNA, Messenger; C515890:Hdac1 protein, rat; D056284:Histone Deacetylase 1