title: Binding of DNA topoisomerases I and II to replication origins.
abstract: The interaction of DNA topology modifying enzymes with eukaryotic DNA replication origins can be detected with nucleotide precision exploiting the action of enzyme poisons specific for type I or type II DNA topoisomerases. Using the topoisomerase I poison camptothecin and the topoisomerase II poison VP16, the precise sites of interaction of these enzymes around the lamin B2 origin have been identified at different points in the cell cycle. The procedure can be applied to any origin for which the sequence has been identified within approximately 1 kb.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
authors.forename: Arturo
authors.initials: A
authors.lastname: Falaschi
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods*; D002166:Camptothecin / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism; D005047:Etoposide / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D034921:Lamin Type B / Q000235:genetics; D008969:Molecular Sequence Data; D011485:Protein Binding; D018741:Replication Origin; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004791:Enzyme Inhibitors; D034921:Lamin Type B; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D005047:Etoposide; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II; D002166:Camptothecin