Chelidonium majus is not hepatotoxic in Wistar rats, in a 4 weeks feeding experiment.
OBJECTIVE
Aerial parts of Chelidonium majus L. (Papaveraceae family) are traditionally used in the treatment of gallstones and dyspepsia, however several cases of hepatotoxicity are reported. In this work we evaluated the effects on liver function of a C. majus extract, obtained from the herbal material responsible for one case of hepatotoxicity.


METHODS
Experiments were performed in Wistar rats, after oral administration of doses corresponding to 1.5 and 3g/(kg day) of herbal drug, for 2 or 4 weeks. Blood samples were collected to perform biochemical analysis, whereas liver samples were used for histomorphological and immunohistochemical examination along with the determination of oxidative stress parameters.


RESULTS
No significant modification in animal body weight, food consumption, enzyme activities, hepatic histomorphology and MDA formation, at either time or dosage level. Conversely, C. majus induced a slight but significant decrease of GSH levels and SOD activity, especially at the high dose.


CONCLUSIONS
Our study suggests that C. majus, at doses about 50 and 100 times higher than those generally used in humans, does not alter hepatic function. However, the reduction in GSH levels and SOD activity suggests particular attention in use of C. majus or its preparations in situations (pharmacological treatments, physio-pathological conditions, etc.) that can compromise liver function.
Mazzanti
Gabriela
G
Department of Physiology and Pharmacology, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy. gabriela.mazzanti@uniroma1.it
Di Sotto
Antonella
A
Franchitto
Antonio
A
Mammola
Caterina Loredana
CL
Mariani
Paola
P
Mastrangelo
Sabina
S
Menniti-Ippolito
Francesca
F
Vitalone
Annabella
A
D000284:Administration, Oral; D000818:Animals; D028281:Chelidonium / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D010936:Plant Extracts; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione