[Study on proteomics of Jurkat cells treated with the extracts from Prunella vulgaris].
OBJECTIVE
To analyse the differential proteins of Jurkat cells after treated with the extracts from Prunella vulgaris using two-dimensional electrophoresis and mass spectrum.


METHODS
Jurkat cell growth inhibitive effect of the extracts from Prunella vulgaris was analyzed by MTT assay. The total proteins of the cells were extracted after treated with the extracts in a dose of 20 microg/mL. Then 2D and MALDI-TOF-MS were used to assess the differential proteins.


RESULTS
The extracts from Prunella vulgaris could depress the proliferation of Jurkat cells in a dose-dependent manner. After 2-DE and MALDI-TOF-MS,11 proteins were identified successfully, including glyceraldehyde-3-phosphate dehydrogenase, coagulation factor VII, Heterogeneous nuclear ribonucleoprotein L, heat shock 70 kDa protein 8 isoform 2, immunoglobulin heavy chain variable region, heterogeneous nuclear ribonucleoprotein A2/B1, zinc finger protein 43, chaperonin containing TCP1, subunit 6A (zeta 1), isoform CRA_b, etc.


CONCLUSIONS
The extracts from Prunella vulgaris could inhibit the growth of Jurkat cells significantly, and lead the proteomics change of Jurkat cells, which may be related to the anti-tumor effect of the extracts from Prunella vulgaris.
Zhang
Ming-Zhi
MZ
Department of Medical Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Sun
Zhen-Chang
ZC
Fu
Xiao-Rui
XR
Chen
Chang-Ying
CY
Ding
Meng-Jie
MJ
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism*; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D040901:Proteomics; D031347:Prunella / Q000737:chemistry*; D012097:Repressor Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D050883:HSC70 Heat-Shock Proteins; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; C474510:ZNF43 protein, human; C439768:hnRNP A2