title: Molecular characterization of Tb, a new approach for an ancient Brucellaphage.
abstract: Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 +/- 2 nm diameter) and short tails (32 +/- 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage's basic mechanisms and practical applications.
authors.affiliation: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.|State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
authors.forename: Cai-Zhong
authors.initials: CZ
authors.lastname: Zhu
authors.affiliation: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Xiong
authors.affiliation: Institute of Combine Injury, School of Preventive Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
authors.forename: Bu-Yun
authors.initials: BY
authors.lastname: Cui
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
authors.forename: Dong-Ri
authors.initials: DR
authors.lastname: Piao
authors.affiliation: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Li
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.affiliation: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Ma
authors.affiliation: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Chai
authors.affiliation: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
authors.forename: Lan-Yu
authors.initials: LY
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D002003:Brucella abortus / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry*; D008969:Molecular Sequence Data; D017902:Podoviridae / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins