Decreased paraoxonase activity in critically ill patients with sepsis.
Paraoxonase 1 is believed to play a role in preventing lipid oxidation and, thus, limiting production of proinflammatory mediators. Systemic inflammatory response in sepsis increases oxidative stress and decreases high-density lipoprotein (HDL) concentrations. The objective of this study was to investigate serum paraoxonase 1 activities in critically ill patients with sepsis and after recovery. Serum paraoxonase 1 arylesterase/paraoxonase activities, concentration of total cholesterol, HDL cholesterol (HDL-C) and serum C-reactive protein (CRP) in septic patients of a medical intensive care unit (n = 30) and age/sex-matched outpatient controls without sepsis (n = 30) were analyzed. Paired convalescent samples were also taken 1 week after recovery (n = 11). In septic patients, both arylesterase (88.3 +/- 36.5 vs. 162.1 +/- 44.8 kU/l, P < 0.001) and paraoxonase (75.2 +/- 50.0 vs. 125.2 +/- 69.4 U/l, P < 0.01) paraoxonase 1 activities decreased as compared to controls. Both activities normalized after recovery. Negative correlation was found between CRP and both arylesterase (r = -0.676, P < 0.001) and paraoxonase (r = -0.401, P < 0.01) as well as positive correlation between HDL-C and both arylesterase (r = 0.585, P < 0.001) and paraoxonase (r = 0.405, P < 0.01) paraoxonase 1 activities. The decreased activity of paraoxonase 1 in negative correlation with CRP offers a potentially useful marker of sepsis progress and recovery in critically ill patients.
Novak
Frantisek
F
4th Department of Internal Medicine, 1st Faculty of Medicine, General Teaching Hospital, Charles University in Prague, U Nemocnice 2, CZ-12808, Prague, Czech Republic. fnova@lf1.cuni.cz
Vavrova
Lucie
L
Kodydkova
Jana
J
Novak
Frantisek
F
Hynkova
Magdalena
M
Zak
Ales
A
Novakova
Olga
O
D000328:Adult; D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers; D002097:C-Reactive Protein / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D016638:Critical Illness; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018805:Sepsis / Q000175:diagnosis* / Q000473:pathology*
D015415:Biomarkers; D008076:Cholesterol, HDL; D002097:C-Reactive Protein; D002784:Cholesterol; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human