title: Preparation of an ochre suppressor tRNA recognizing exclusively UAA codon by using the molecular surgery technique.
abstract: In order to create an ochre suppressor tRNA which exclusively recognizes UAA codon, we replaced the G34 at the first position of yeast tRNA(Tyr)[GPsiA] anticodon with pseudouridine34 (Psi34) by using the molecular surgery technique. This tRNA(Tyr)[PsiPsiA] recognized only the UAA codon as expectedly, but tRNA(Tyr)[UPsiA] made as a control also behaved similarly. This result may suggest that U34 must be somehow modified to facilitate the wobble-pairing to G at the third position of codon.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokogawa
authors.forename: Hanim Munirah Binti Che
authors.initials: HM
authors.lastname: Hassan
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yokota
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohno
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nishikawa
mesh_terms: D000926:Anticodon / Q000737:chemistry; D018388:Codon, Terminator / Q000737:chemistry*; D005821:Genetic Techniques; D011560:Pseudouridine / Q000737:chemistry; D012365:RNA, Transfer, Tyr / Q000737:chemistry*
chemical_list: D000926:Anticodon; D018388:Codon, Terminator; D012365:RNA, Transfer, Tyr; D011560:Pseudouridine