Protein and metabolite analysis reveals permanent induction of stress defense and cell regeneration processes in a tobacco cell suspension culture.
The secretome of a tobacco cell suspension culture was investigated by a combined proteomic and metabolomic approach. Protein analysis from 2-DE gels led to identification of 32 out of 60 spots from culture medium. Identified proteins were mainly involved in stress defence and cell regeneration processes. Among them three putative new isoforms, e.g. for chitinase, peroxidase and beta-1,4-xylosidase were identified, not yet present in available protein databases for the genus Nicotiana. GC-MS analysis of time course experiments revealed significant changes for metabolites involved in energy transport, signalling and cell development. Among them, the most significant increase was found for putrescine in the medium of cultures entering the exponential phase. Results showed strong abundance of stress associated proteins and metabolites in the absence of elicitors or additional stress treatments.
Lippmann
Rico
R
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
Kaspar
Stephanie
S
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
Rutten
Twan
T
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
Melzer
Michael
M
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
Kumlehn
Jochen
J
0000-0001-7080-7983
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
Matros
Andrea
A
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
Mock
Hans-Peter
HP
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
D000595:Amino Acid Sequence; D048708:Cell Growth Processes / Q000235:genetics; D002460:Cell Line; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D055442:Metabolome; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism
D007527:Isoenzymes; D010940:Plant Proteins; D020543:Proteome; D010544:Peroxidases; D014995:Xylosidases; D002688:Chitinases; C026579:exo-1,4-beta-D-xylosidase; D011700:Putrescine