An unusual dependence of human herpesvirus-8 glycoproteins-induced cell-to-cell fusion on heparan sulfate.
Human herpesvirus-8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins-induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from following lines of evidence: (1) a significant reduction in cell-to-cell fusion occurred when target cells were treated with heparinase; (2) in a competition assay, when the effector cells expressing HHV-8 glycoproteins were challenged with soluble HS, cell-to-cell fusion was reduced; and, (3) co-expression of HHV-8 glycoproteins gH-gL on target cells resulted in inhibition of cell surface HS expression. Taken together, our results indicate that cell surface HS can play an additional role during HHV-8 pathogenesis.
Tiwari
Vaibhav
V
Department of Ophthalmology, University of Illinois at Chicago, IL 60612, USA.
Darmani
Nissar A
NA
Thrush
Gerald R
GR
Shukla
Deepak
D
D000818:Animals; D016466:CHO Cells; D002459:Cell Fusion; D002462:Cell Membrane / Q000378:metabolism / Q000821:virology; D006224:Cricetinae; D003412:Cricetulus; D006497:Heparan Sulfate / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000378:metabolism*; D019288:Herpesvirus 8, Human / Q000378:metabolism*; D006801:Humans; D008561:Membrane Fusion; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D006497:Heparan Sulfate; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116207:glycoprotein B, human herpesvirus 8; C464461:glycoprotein H, human herpesvirus 8; C534114:glycoprotein L, human herpesvirus 8