Edaravone protects against apoptotic neuronal cell death and improves cerebral function after traumatic brain injury in rats.
Edaravone is a novel free radical scavenger used clinically in patients with acute cerebral infarction; however, it has not been assessed in traumatic brain injury (TBI). We investigated the effects of edaravone on cerebral function and morphology following TBI. Rats received TBI with a pneumatic controlled injury device. Edaravone (3 mg/kg) or physiological saline was administered intravenously following TBI. Numbers of 8-OHdG-, 4-HNE-, and ssDNA-positive cells around the damaged area after TBI were significantly decreased in the edaravone group compared with the saline group (P < 0.01). There was a significant increase in neuronal cell number and improvement in cerebral dysfunction after TBI in the edaravone group compared with the saline group (P < 0.01). Edaravone administration following TBI inhibited free radical-induced neuronal degeneration and apoptotic cell death around the damaged area. In summary, edaravone treatment improved cerebral dysfunction following TBI, suggesting its potential as an effective clinical therapy.
Itoh
Tatsuki
T
Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, Osakasayama, Osaka 589-8511, Japan. tatsuki@med.kindai.ac.jp
Satou
Takao
T
Nishida
Shozo
S
Tsubaki
Masahiro
M
Imano
Motohiro
M
Hashimoto
Shigeo
S
Ito
Hiroyuki
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D001930:Brain Injuries / Q000188:drug therapy*; D004277:DNA, Single-Stranded / Q000276:immunology / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D051959:ELAV Proteins / Q000276:immunology; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology; D018782:Maze Learning / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D000447:Aldehydes; D004277:DNA, Single-Stranded; D051959:ELAV Proteins; D016166:Free Radical Scavengers; D005904:Glial Fibrillary Acidic Protein; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C027576:4-hydroxy-2-nonenal; D000077553:Edaravone; D000983:Antipyrine