Comparison of nucleic acid extraction methods for the detection of Mycoplasma pneumoniae.
Four nucleic acid extraction procedures (2 automated and 2 manual) were compared for their efficiency at isolating Mycoplasma pneumoniae DNA. Oropharyngeal swabs from healthy volunteers were spiked with varying amounts of M. pneumoniae, extracted, and tested using real-time polymerase chain reaction. Our data indicate that both automated extraction methods consistently outperform the manual procedures.
Thurman
Kathleen A
KA
Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Cowart
Kelley C
KC
Winchell
Jonas M
JM
D001331:Automation; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D006801:Humans; D009175:Mycoplasma Infections / Q000175:diagnosis*; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods
D004269:DNA, Bacterial