Simultaneous determination of seven components in Qibaomeiran pill by HPLC-DAD.
A high-performance liquid chromatography coupled with photo diode array detection method is developed for simultaneous determination of seven active components in Qibaomeiran pill, including rutin, 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glycoside, ferulic acid, psoralen, isopsoralen, emodin, and physcion. The analysis is performed on a C(18) column using a mobile phase composed of A (0.1% acetic acid) and B (acetonitrile) with linear gradient elution. Four wavelengths at 245, 290, 320, and 350 nm were chosen as the monitoring wavelength to determine seven active components, respectively. All the compounds show good linearity (r > 0.999). The developed method is fully validated in respect to precision, repeatability, and accuracy. The proposed method is successfully applied to quantify the seven active components in different Qibaomeiran pill samples. The results indicate that the developed assay could be considered as a suitable quality control method for the Qibaomeiran pill.
Wang
Chunying
C
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, PR China.
Wang
Qiao
Q
Yuan
Zhifang
Z
Yang
Wei
W
Xie
Fengli
F
Zhang
Lantong
L
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D011564:Furocoumarins / Q000032:analysis / Q000737:chemistry; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; D006027:Glycosides; C000597912:qibaomeiran; C004999:ferulic acid; C011659:angelicin; C008905:physcione; D004642:Emodin