title: The extracellular matrix protein SC1/Hevin localizes to multivesicular bodies in Bergmann glial fibers in the adult rat cerebellum.
abstract: SC1 is an extracellular matrix molecule prominent in the mammalian brain. In the cerebellum, SC1 localizes to Bergmann glial cells and perisynaptic glial processes that envelop synapses in the molecular layer. In the present study, confocal microscopy revealed a punctate distribution of SC1 along Bergmann glial fibers that colocalized with the intermediate filament GFAP when fibers were viewed in cross-section. Immunoelectron microscopy showed that the punctate SC1 pattern corresponded to the localization of SC1 in multivesicular bodies situated within Bergmann glial fibers. The pattern of SC1 localization was not disrupted following hyperthermia or pilocarpine-induced status epilepticus. The present study suggests that SC1 protein may reach its destination in perisynaptic glial processes and glial endfeet by transport along Bergmann glial fibers in multivesicular bodies and that this process is preserved following stress.
authors.affiliation: Center for the Neurobiology of Stress, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.
authors.forename: Starlee
authors.initials: S
authors.lastname: Lively
authors.forename: Ian R
authors.initials: IR
authors.lastname: Brown
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006979:Hyperthermia, Induced; D008297:Male; D016253:Microscopy, Immunoelectron; D057146:Multivesicular Bodies / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology; D013569:Synapses / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D005904:Glial Fibrillary Acidic Protein; C503128:Sparcl1 protein, rat; D010862:Pilocarpine