Production of pure beta-glucan by Aureobasidium pullulans after pullulan synthetase gene disruption.
By disruption of the pullulan synthetase gene (pul) of Aureobasidium pullulans IMS822 KCTC11179BP, we constructed a mutant strain, A. pullulans NP1221, which produced a pure beta-glucan exopolysaccharide. The mutant NP1221 was white, whereas the wild-type strain produced a black dye. When we compared fermentation kinetics between wide-type and mutant strains, the mutant NP1221 did not produce pullulan. Substrate uptake rate and beta-glucan production were similar in both strains. However, the biomass yield of mutant NP1221 was 2.3-fold (9.2 g l(-1)) greater than that of wild-type.
Kang
Byung-Kwan
BK
Institute Bioindustry Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 1404, Sinjeong-dong, Jeungeup-si, Jeonbuk, 580-185, Korea.
Yang
Hee-Jong
HJ
Choi
Nack-Shick
NS
Ahn
Keug-Hyun
KH
Park
Chan-Sun
CS
Yoon
Byung-Dae
BD
Kim
Min-Soo
MS
D001203:Ascomycota / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005936:Glucans / Q000235:genetics*; D007700:Kinetics; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D047071:beta-Glucans / Q000378:metabolism*
D005656:Fungal Proteins; D005936:Glucans; D047071:beta-Glucans; C009109:pullulan