A colorimetric and fluorescent merocyanine-based probe for biological thiols.
A new "dual-mode" chromogenic and fluorescent turn-on probe (2) for the selective sensing of biological thiols is reported. In MeOH-H(2)O cosolvent at physiological pH 7.40 (MeOH-H(2)O = 3:7), biological thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the chromo- and fluorophore merocyanine (3).
Wang
Shu-Ping
SP
Department of Chemistry and The Key Laboratory of Analytical Sciences of MOE, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, PR China.
Deng
Wu-Jian
WJ
Sun
Dan
D
Yan
Min
M
Zheng
Hong
H
Xu
Jin-Gou
JG
D003124:Colorimetry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011744:Pyrimidinones / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry
D005456:Fluorescent Dyes; D011744:Pyrimidinones; D013438:Sulfhydryl Compounds; C003954:merocyanine dye