Synthesis and characterization of deoxyuridine triphosphates labeled with pyrene.
Deoxyuridine triphosphate derivatives modified with pyrene was synthesized to functionalize DNA with fluorescent molecules based on the template DNA sequence. Incorporation of pyrene-labeled deoxyuridine triphosphates into DNA by DNA polymerase was investigated by using reverse-phase HPLC and polyacrylamide gel electrophoresis. The fluorescent properties of functionalized DNA were characterized by the steady-state fluorescence measurements.
Tanimizu
Yosuke
Y
Graduate School of Engineering, University of Hyogo, Himeji, Hyogo 671-2280, Japan.
Takada
Tadao
T
Nakamura
Mitsunobu
M
Yamana
Kazushige
K
D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*
D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D011721:Pyrenes; D004247:DNA; D004259:DNA-Directed DNA Polymerase