title: The immunohistochemical localization of the glycosphingolipid asialo-GM1 in the intestine of weaned piglets.
abstract: The duodenum, jejunum, ileum, cecum and colon of three male hybrid piglets, 4 weeks old just after weaning, were investigated for the immunohistochemical localization of the asialoganglioside, GM1 (asialo-GM1). The study revealed various degrees of labelling for this acid glycosphingolipid in neural, epithelial and blood elements in all the gut segments. The immunolabelled neural structures, represented by ganglionic perikarya and nerve fibers, were distributed throughout the intestinal wall and showed quantitative variations in the various regions. In contrast the numerical evaluation of labelled epithelial cells was encountered only in the terminal jejunum and along the entire ileum, cecum and large intestine. In addition, a heterogeneous population of immunolabelled leukocytes was spread randomly in the lamina propria and submucosa of the entire intestine and did not show any apparent quantitative fluctuations between the different parts. The observations regarding the typical distribution patterns of the asialoganglioside GM1 in ganglionic perikarya and epithelial cells of weaned piglets are discussed in relation to their possible functional significance in the intestine and other mammalian organs.
authors.affiliation: Center of Cytobiology, Institute of Biology, Spl Independentei 296, Bucharest, Romania. ioana.trandaburu@ibiol.ro
authors.forename: Ioana
authors.initials: I
authors.lastname: Trandaburu
authors.forename: Isabelle P
authors.initials: IP
authors.lastname: Oswald
authors.forename: Tiberiu
authors.initials: T
authors.lastname: Trandaburu
mesh_terms: D016604:Aflatoxin B1 / Q000008:administration & dosage; D000818:Animals; D037341:Fumonisins / Q000008:administration & dosage; D005677:G(M1) Ganglioside / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000737:chemistry*; D008297:Male; D013552:Swine; D014886:Weaning
chemical_list: D037341:Fumonisins; D005677:G(M1) Ganglioside; C056933:fumonisin B1; D016604:Aflatoxin B1