title: A molecular model for diacylglycerol acyltransferase from Mortierella ramanniana var. angulispora.
abstract: Acyl CoA diacylglycerol acyltransferase (DGAT, EC 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. It catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. The protein sequence of diacylglycerol acyltransferse (DGAT) Type 2B in Moretierella ramanniana var. angulispora (Protein_ID = AAK84180.1) was retrieved from GenBank. However, a structure is not yet available for this sequence. The 3D structure of DGAT Type 2B was modeled using a template structure (PDB ID: 1K30) obtained from Protein databank (PDB) identified by searching with position specific iterative BLAST (PSI-BLAST). The template (PDB ID: 1K30) describes the structure of DGAT from Cucurbita moschata. Modeling was performed using Modeller 9v2 and protein model is hence generated. The DGAT type 2B protein model was subsequently docked with six inhibitors (sphingosine; trifluoroperazine; phosphatidic acid; lysophospatidylserine; KCl; 1, 2-diolein) using AutoDock (a molecular docking program). The binding of inhibitors to the protein model of DGAT type 2B is discussed.
authors.affiliation: Department of Biotechnology, College of Engineering and Technology, IFTM Campus, Lodhipur-Rajput, Delhi Road, Moradabad 244 001, U.P., India. sanjaymishra66@gmail.com
authors.forename: Sanjay
authors.initials: S
authors.lastname: Mishra
authors.forename: Surya Prakash
authors.initials: SP
authors.lastname: Dwivedi
authors.forename: Neeraja
authors.initials: N
authors.lastname: Dwivedi
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.forename: Anil
authors.initials: A
authors.lastname: Rawat
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kamisaka