title: Expression profiles of seven glutathione S-transferase (GST) genes in cadmium-exposed river pufferfish (Takifugu obscurus).
abstract: Glutathione S-transferase (GST; EC 2.5.1.18) plays a critical role in detoxification pathways. In this study, we report cloning and expression of seven genes of the GST family of the pufferfish Takifugu obscurus together with mRNA tissue distribution pattern and time-course of expression in response to exposure to cadmium. At basal levels of tissue expression, GST-Mu is highly expressed in liver compared with other tissues. When fish were exposed to cadmium (5 mg/L for 96 h), expression of GST-MAPEG, GST-Mu, GST-Omega, and GST-Zeta was greatly increased, whereas GST-Alpha and GST-Kappa genes showed no significant response. These findings suggest that gene expression of a number of GST isoforms in T. obscurus is modulated in response to exposure to cadmium. We propose GST-Mu, GST-Theta, and GST-Zeta as candidate biomarkers for heavy metal exposure in this fish.
authors.affiliation: National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea.
authors.forename: Jin-Hyoung
authors.initials: JH
authors.lastname: Kim
authors.forename: Hans-Uwe
authors.initials: HU
authors.lastname: Dahms
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
authors.forename: Kyung-Nam
authors.initials: KN
authors.lastname: Han
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003001:Cloning, Molecular; D004784:Environmental Monitoring; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005819:Genetic Markers; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D023741:Takifugu / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D005819:Genetic Markers; D007527:Isoenzymes; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D002104:Cadmium; D005982:Glutathione Transferase