Bioactive steroidal sulfates from the ambulakrums of the Pacific starfish Lysastrosoma anthosticta.
Lysaketotriol (1) and lysaketodiol (2), along with two previously known polyhydroxylated steroidal sulfates (3, 4), were isolated from the ethanolic extract of ambulakrums separated from the arms of the starfish Lysastrosoma anthosticta (Sea of Japan). The chemical structures have been elucidated using 1D and 2D NMR spectroscopy (1H, 13C, DEPT, COSY-45, HSQC and HMBC) and mass spectrometry. Compound 1 produced moderate stimulation of lysosomal activity in mouse splenocytes and compounds 1-2 showed immunomodulatory activity.
Levina
Eleonora V
EV
Pacific Institute of Bioorganic Chemistry, Vladivostok, Russian Federation. levina@piboc.dvo.ru
Kalinovsky
Anatoly I
AI
Dmitrenok
Pavel S
PS
Aminin
Dmitry L
DL
D000818:Animals; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D010140:Pacific Ocean; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D013154:Spleen; D013215:Starfish / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D012503:Saponins; D013256:Steroids; D013431:Sulfates