title: Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases.
abstract: We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531, Boelter Hall, 420 Westwood Plaza, Los Angeles, CA 90095, USA.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yan
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Lee
authors.forename: James C
authors.initials: JC
authors.lastname: Liao
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002072:Butylene Glycols / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D002072:Butylene Glycols; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP)