title: Effect of catalase and superoxide dismutase on motility, viability and acrosomal integrity of frozen-thawed cat spermatozoa.
abstract: The aim of this study was to evaluate the effect of antioxidant catalase (CAT) and superoxide dismutase (SOD) in semen extender on motility, viability and acrosomal integrity of frozen-thawed cat spermatozoa. Semen was collected by using an artificial vagina from five domestic cats (two ejaculates/cat). Spermatozoa were diluted in egg yolk Ttris-fructose citrate solution (EYT-FC) without glycerol and cooled at 4 degrees C for 1 h, then diluted further with EYT-FC with glycerol (7% final concentration) and 400 IU/ml of CAT (treatment 1) or SOD (treatment 2) or without antioxidants (control). Before freezing using a styrofoam box, diluted spermatozoa filled in 0.25-ml straws were equilibrated for 1 h at 4 degrees C. After thawing, spermatozoa were assessed for motility, viability and acrosomal integrity. Cryopreservation significantly impaired sperm motility, viability and acrosomal integrity (p < 0.05). However, motility, viability and acrosomal integrity of frozen-thawed cat spermatozoa in the EYT-FC with CAT, SOD and without the antioxidants were not significantly different. The average percentages of spermatozoa motility after thawing compared between control, treatment 1 and treatment 2 group were 43.5 +/- 3.2, 42 +/- 4.1 and 38 +/- 4.5; for viability: 44.8 +/- 3.5, 50.6 +/- 5.7 and 47.1 +/- 4.1 and for acrosomal integrity: 45 +/- 3.5, 44.9 +/- 3.4 and 44.4 +/- 3.3, respectively. In conclusion, adding CAT and SOD to EYT-FC did not improve motility, viability and acrosomal integrity in cryopreserved cat spermatozoa.
authors.affiliation: Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom, Thailand. fvetkot@yahoo.com
authors.forename: K
authors.initials: K
authors.lastname: Thiangtum
authors.forename: A
authors.initials: A
authors.lastname: Pinyopummin
authors.forename: T
authors.initials: T
authors.lastname: Hori
authors.forename: E
authors.initials: E
authors.lastname: Kawakami
authors.forename: T
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002374:Catalase / Q000494:pharmacology*; D002415:Cats; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000494:pharmacology*
chemical_list: D003451:Cryoprotective Agents; D002374:Catalase; D013482:Superoxide Dismutase