title: Angiotensin type 1 receptor expression and interleukin-8 production in polymorphonuclear leukocytes of patients with peripheral arterial disease.
abstract: We investigated angiotensin type 1 receptor (AT1R) expression and interleukin-8 (IL-8) productions in polymorphonuclear leukocytes obtained from patients with peripheral arterial disease (PAD) undergoing femoral endarterectomy. Subjects at high cardiovascular risk (high-risk subjects, HRS) and healthy controls (HC) were also enrolled. To this end, patients with PAD were studied 1 month before surgery, at the time of surgery, and 3 and 6 months after surgery. Polymorphonuclear leukocytes were obtained from venous blood and evaluated for AT1R expression at messenger RNA (mRNA) and protein level and IL-8 production (by means of enzyme-linked immunosorbent assay). At baseline, AT1R membrane expression was similar in cells from patients with PAD, HRS, and HC, whereas AT1R mRNA was similar in patients with PAD and HC and higher in HRS. During the follow-up period, AT1R expression progressively decreased both on the cell membrane and at the mRNA level. Both resting and stimulated production of IL-8 was lower in patients with PAD in comparison to HC and HRS and did not change during the follow up period. In PAD patients, femoral endarterectomy is associated with reduction of AT1R expression however with no apparent effect on IL-8 production. The relevance of such effects for cardiovascular protection deserves consideration.
authors.affiliation: Department of Clinical Medicine, Section of Experimental and Clinical Pharmacology, University of Insubria, Varese VA, Italy. franca.marino@uninsubria.it
authors.forename: Franca
authors.initials: F
authors.lastname: Marino
authors.forename: Luigina
authors.initials: L
authors.lastname: Guasti
authors.forename: Matteo
authors.initials: M
authors.lastname: Tozzi
authors.forename: Ramona
authors.initials: R
authors.lastname: Consuelo Maio
authors.forename: Luana
authors.initials: L
authors.lastname: Castiglioni
authors.forename: Emanuela
authors.initials: E
authors.lastname: Rasini
authors.forename: Laura
authors.initials: L
authors.lastname: Schembri
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Maroni
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Legnaro
authors.forename: Alessandra
authors.initials: A
authors.lastname: De Leo
authors.forename: Gabriele
authors.initials: G
authors.lastname: Piffaretti
authors.forename: Patrizio
authors.initials: P
authors.lastname: Castelli
authors.forename: Achille
authors.initials: A
authors.lastname: Venco
authors.forename: Sergio
authors.initials: S
authors.lastname: Lecchini
authors.forename: Marco
authors.initials: M
authors.lastname: Cosentino
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000924:Anticholesteremic Agents / Q000627:therapeutic use; D001054:Apolipoproteins A / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D050197:Atherosclerosis / Q000097:blood* / Q000188:drug therapy / Q000601:surgery; D000069059:Atorvastatin; D002318:Cardiovascular Diseases / Q000097:blood; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004691:Endarterectomy; D005260:Female; D005263:Femoral Artery / Q000601:surgery; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006538:Heptanoic Acids / Q000627:therapeutic use; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D016491:Peripheral Vascular Diseases / Q000097:blood* / Q000188:drug therapy / Q000601:surgery; D011758:Pyrroles / Q000627:therapeutic use; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D000924:Anticholesteremic Agents; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D006538:Heptanoic Acids; D016209:Interleukin-8; D011758:Pyrroles; D044140:Receptor, Angiotensin, Type 1; D014280:Triglycerides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002784:Cholesterol; D000069059:Atorvastatin