Flow injection chemiluminescence determination of 6-mercaptopurine based on a new system of potassium permanganate-thioacetamide-sodium hexametaphosphate.
A novel chemiluminescence method for the determination of 6-mercaptopurine was established based on 6-mercaptopurine inhibition of the chemiluminescence emission of potassium permanganate-thioacetamide-sodium hexametaphosphate system. The peak height was proportional to log 6-mercaptopurine concentration in the range 7.0 × 10(-10) to 1.0 × 10(-7)  g/mL and the detection limit was 1.9 × 10(-11)  g/mL (S/N = 3). The relative standard deviation was 1.5% for the determination of 8.0 × 10(-8)  g/mL 6-mercaptopurine (n = 11). The proposed sensor was successfully applied to the analysis of 6-mercaptopurine in human serum samples.
Wang
Lun
L
Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, China. WangLun@mail.ahnu.edu.cn
Ling
Bo
B
Chen
Hongqi
H
Liang
Ani
A
Qian
Binbin
B
Fu
Jie
J
D017022:Flow Injection Analysis / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D015122:Mercaptopurine / Q000032:analysis / Q000097:blood*; D010710:Phosphates; D011196:Potassium Permanganate; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013853:Thioacetamide
D007202:Indicators and Reagents; D010710:Phosphates; D011196:Potassium Permanganate; D013853:Thioacetamide; D015122:Mercaptopurine; C009285:sodium polymetaphosphate