Synthesis and properties of oligodeoxynucleotides containing biaryl units.
We describe the synthesis and properties of oligonucleotides (ONs) containing biaryl units, which are composed of a bis(hydroxymethyl)benzene residue and a naphthalene or pyrene moiety. We found that by introducing the biaryl units into the ONs, the aromatic chromophores are suitably arrayed in the DNAs. Further, we succeeded in the detection of a single-base mismatch in RNA by using the ON containing the biaryl unit as a molecular beacon.
Ueno
Yoshihito
Y
Department of Biomolecular Science, Faculty of Engineering, Gifu University, United Graduate School of Drug Discovery and Medical Information Sciences, Gifu 501-1193, Japan. uenoy@gifu-u.ac.jp
Komatsuzaki
Shinji
S
Takasu
Keiji
K
Kitamura
Yoshiaki
Y
Kitade
Yukio
Y
D001592:Benzyl Alcohols / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D001592:Benzyl Alcohols; D005456:Fluorescent Dyes; D009281:Naphthalenes; D009838:Oligodeoxyribonucleotides; D011721:Pyrenes; D012333:RNA, Messenger