title: Doppler ultrasonography for the noninvasive measurement of uterine artery volume blood flow through gestation in the pregnant sheep.
abstract: Accurate noninvasive quantification of volume blood flow in the uterine arteries (UtAs) would have clinical and research benefits. We evaluated the correlation and agreement between uterine artery volume blood flow (UtABF) as calculated (cUtABF) from color/pulsed-wave Doppler acquisitions and that measured (mUtABF) by bilateral perivascular transit-time flow probes in 6 pregnant sheep at 2 gestational ages. Out of 22 Doppler acquisitions, 19 were successful. The overall correlation between cUtABF and mUtABF was 0.55 (n = 19, P = .01). Calculated UtABF and mUtABF were significantly correlated in late gestation (n = 11, r = 0.71, P = .01) but not at mid-gestation (n = 8, r = .02, P = .96). By Bland-Altman analysis, the mean cUtABF/mUtABF was 1.15 with 95% limit of agreement (-0.26 to 2.56), similar to results previously achieved using power/pulsed-wave Doppler. Despite the acceptable correlation, the limits of agreement between Doppler and transit-time flow probe measurements remain wide. This makes Doppler ultrasonography less than a desirable method to quantify UtABF in studies where accurate quantification is required.
authors.affiliation: Fetal Medicine Unit, Elizabeth Garrett Anderson Institute for Women's Health, University College London and University College London Hospitals NHS Foundation Trust, London WC1E 6HX, United Kingdom. abinaderk@yahoo.com
authors.forename: Khalil N
authors.initials: KN
authors.lastname: Abi-Nader
authors.forename: Vedanta
authors.initials: V
authors.lastname: Mehta
authors.forename: Victoria
authors.initials: V
authors.lastname: Wigley
authors.forename: Elisa
authors.initials: E
authors.lastname: Filippi
authors.forename: Berrin
authors.initials: B
authors.lastname: Tezcan
authors.forename: Michael
authors.initials: M
authors.lastname: Boyd
authors.forename: Donald M
authors.initials: DM
authors.lastname: Peebles
authors.forename: Anna L
authors.initials: AL
authors.lastname: David
mesh_terms: D000818:Animals; D001810:Blood Volume / Q000502:physiology*; D005260:Female; D011247:Pregnancy; D012039:Regional Blood Flow / Q000502:physiology*; D012044:Regression Analysis; D012756:Sheep; D018608:Ultrasonography, Doppler / Q000379:methods*; D016216:Ultrasonography, Prenatal / Q000379:methods; D055988:Uterine Artery / Q000000981:diagnostic imaging* / Q000502:physiology