title: Structural insights into the mechanism of the allosteric transitions of Mycobacterium tuberculosis cAMP receptor protein.
abstract: The cAMP receptor protein (CRP) from Mycobacterium tuberculosis is a cAMP-responsive global transcriptional regulator, responsible for the regulation of a multitude of diverse proteins. We have determined the crystal structures of the CRP.cAMP and CRP.N(6)-cAMP derivative-bound forms of the enzyme to 2.2- and 2.3 A-resolution, respectively, to investigate cAMP-mediated conformational and structural changes. The allosteric switch from the open, inactive conformation to the closed, active conformation begins with a number of changes in the ligand-binding cavity upon cAMP binding. These subtle structural changes and numerous non-bonding interactions between cAMP, the N-domain residues, and the C-domain helices demonstrate that the N-domain hairpin loop acts as a structural mediator of the allosteric switch. Based on the CRP.N(6)-cAMP crystal structure, binding of N(6)-cAMP with a bulkier methylphenylethyl extension from the N6 atom stabilizes the cAMP-binding domain, N-domain hairpin, and C-terminal domain in a similar manner as that of the CRP.cAMP structure, maintaining structural integrity within the subunits. However, the bulkier N6 extension of N(6)-cAMP (in R conformation) is accommodated only in subunit A with minor changes, whereas in subunit B, the N6 extension is in the S conformation hindering the hinge region of the central helix. As a result, the entire N-domain and the C-domain of subunit B integrated by the cAMP portion of this ligand, together tilt away ( approximately 7 degrees tilt) from central helix C, positioning the helix-turn-helix motif in an unfavorable position for the DNA substrate, asymmetrically. Together, these crystal structures demonstrate the mechanism of action of the cAMP molecule and its role in integrating the active CRP structure.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843.
authors.forename: Manchi C M
authors.initials: MCM
authors.lastname: Reddy
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843.
authors.forename: Satheesh K
authors.initials: SK
authors.lastname: Palaninathan
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843.
authors.forename: John B
authors.initials: JB
authors.lastname: Bruning
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843.
authors.forename: Cory
authors.initials: C
authors.lastname: Thurman
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843.
authors.forename: Danielle
authors.initials: D
authors.lastname: Smith
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843. Electronic address: sacchett@tamu.edu.
authors.forename: James C
authors.initials: JC
authors.lastname: Sacchettini
mesh_terms: D000494:Allosteric Regulation / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D000242:Cyclic AMP / Q000737:chemistry* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary / Q000502:physiology; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; C513697:CRP protein, Mycobacterium tuberculosis; D000242:Cyclic AMP