title: Plate assay for fungal enzymes using cellophane membranes.
abstract: Fungal mycelia mass and pigments are major obstacles to investigating the secretion of bioactive substances such as enzyme activities using a plate assay. In this study, we applied a cellophane membrane and demonstrated that it can block mycelia mass and conidia (especially pigmented spores that would likely interfere with any subsequent color development-based activity detection) while allowing secreting enzymes to pass through. Visual observation after lifting the cellophane membrane and the collected mycelia and conidia indicated that the bioactivities on specific plates were improved significantly, although some fungal growth hurdle was noted. This proved to be true whether the assays were color development based or not.
authors.affiliation: Department of Molecular Biotechnology, Da-Yeh University, Changhua 51591, Taiwan.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Liu
authors.forename: Pei-Char
authors.initials: PC
authors.lastname: Feng
authors.forename: Chaur-Tsuen
authors.initials: CT
authors.lastname: Lo
authors.forename: Kou-Cheng
authors.initials: KC
authors.lastname: Peng
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry; D002476:Cellophane / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism*; D025282:Mycelium / Q000201:enzymology; D013172:Spores, Fungal / Q000201:enzymology; D014242:Trichoderma / Q000201:enzymology
chemical_list: D002130:Calcium Phosphates; D002476:Cellophane; D002480:Cellulase; C018392:tricalcium phosphate