title: Methylhexahydrophthalic anhydride adducted albumin tryptic peptides in nasal lavage fluid.
abstract: Methylhexahydrophthalic anhydride (MHHPA) is a reactive, low molecular weight chemical used in products such as plastics, paints, and electronic components. Exposure to MHHPA may lead to work-related airway diseases such as rhinitis, conjunctivitis, and asthma. Twelve subjects employed at a plant manufacturing electrical capacitors using MHHPA were included in this study. Nasal lavages were collected from subjects before work Monday morning and after work Tuesday afternoon. The levels of MHHPA adducted to serum albumin were analyzed with a straightforward work-up method. The samples were trypsinated before being analyzed with a liquid chromatography-triple quadrupole mass spectrometer. The mass spectrometer was run using selected reaction monitoring for six adducted peptides. Also, some biomarkers of effect (albumin, total protein, eosinophil cationic protein, and tryptase) were analyzed in nasal lavages. Furthermore, the metabolite MHHP acid in urine after work on Tuesday was analyzed by gas chromatography-mass spectrometry. Symptoms from the airways and the eyes and sensitization were registered. The main result of this study is that protein adducts can be analyzed in vivo after low occupational exposures to MHHPA. The results also show a correlation between adducted peptides and albumin in nasal lavage. Furthermore, there may be a difference in the potential to induce hyperresponsiveness between adducts bound to different amino acids.
authors.affiliation: Department of Occupational and Environmental Medicine, Institute of Laboratory Medicine, University Hospital, Lund, Sweden. Marina.Jeppsson@med.lu.se
authors.forename: Marina C
authors.initials: MC
authors.lastname: Jeppsson
authors.forename: Christian H
authors.initials: CH
authors.lastname: Lindh
authors.forename: Monica H
authors.initials: MH
authors.lastname: Kristiansson
authors.forename: Jörn
authors.initials: J
authors.lastname: Nielsen
authors.forename: Bo A G
authors.initials: BA
authors.lastname: Jönsson
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000737:chemistry* / Q000633:toxicity*; D000418:Albumins / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry; D015415:Biomarkers; D016535:Bronchial Hyperreactivity / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D047150:Eosinophil Cationic Protein / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D019570:Inhalation Exposure; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009296:Nasal Cavity / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D010796:Phthalic Anhydrides / Q000737:chemistry* / Q000633:toxicity* / Q000652:urine; D010865:Pilot Projects; D011492:Protein Hydrolysates / Q000737:chemistry*; D007507:Therapeutic Irrigation; D014357:Trypsin / Q000737:chemistry*; D053802:Tryptases / Q000032:analysis
chemical_list: D000395:Air Pollutants, Occupational; D000418:Albumins; D000596:Amino Acids; D015415:Biomarkers; D010455:Peptides; D010796:Phthalic Anhydrides; D011492:Protein Hydrolysates; C051070:4-methylcyclohexyl-1,6-dicarboxylic acid anhydride; D047150:Eosinophil Cationic Protein; D014357:Trypsin; D053802:Tryptases