Statistical assessment of variability of terminal restriction fragment length polymorphism analysis applied to complex microbial communities.
The variability of terminal restriction fragment polymorphism analysis applied to complex microbial communities was assessed statistically. Recent technological improvements were implemented in the successive steps of the procedure, resulting in a standardized procedure which provided a high level of reproducibility.
Rossi
Pierre
P
Ecole Polytechnique Fédérale de Lausanne, ENAC ISTE Laboratory for Environmental Biotechnology, CH C3 425, Station 6, CH-1015 Lausanne, Switzerland. pierre.rossi@epfl.ch
Gillet
François
F
Rohrbach
Emmanuelle
E
Diaby
Nouhou
N
Holliger
Christof
C
D054458:Amplified Fragment Length Polymorphism Analysis / Q000592:standards / Q000706:statistics & numerical data*; D001419:Bacteria / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D014644:Genetic Variation; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S