title: Examination of the brain mitochondrial lipidome using shotgun lipidomics.
abstract: Contamination from subcellular organelles and myelin has hindered attempts to characterize the lipidome of brain mitochondria. A high degree of mitochondrial purity is required for accurate measurements of the content and molecular species composition of mitochondrial lipids. We devised a discontinuous Ficoll and sucrose gradient procedure for the isolation and purification of brain mitochondria free from any detectable contamination. Shotgun lipidomics was used to analyze the lipid composition of the brain mitochondria. These procedures can be used to determine whether intrinsic lipid abnormalities underlie mitochondrial dysfunction associated with neurological and neurodegenerative diseases.
authors.affiliation: Biology Department, Boston College, Boston, MA, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kiebish
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Seyfried
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*
chemical_list: D008055:Lipids