title: Selection of high affine peptide ligands for detection of Clostridium Tyrobutyricum spores.
abstract: Clostridium tyrobutyricum is the main agent responsible for "late blowing" in cheese, which causes severe economic losses. Nowadays, the reference method for its detection is the Most-Probable-Number (MPN); however, it is time consuming and non-specific. Thus, in order to check milk contamination with spores of C. tyrobutyricum, a more specific and rapid method would be required. The objective of this work was to obtain a ligand to establish the basis to develop a biomagnetic separation method for detection of C. tyrobutyricum spores. This study describes the selection of thirteen highly affine peptides to C. tyrobutyricum spores from a phage-display peptide library. In order to test the ability of the peptides attached to a solid support to bind the spores, the most frequent peptide was synthesised and used to coat paramagnetic beads.
authors.affiliation: Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Lavilla
authors.forename: Ruth
authors.initials: R
authors.lastname: De Luis
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Pérez
authors.forename: Miguel
authors.initials: M
authors.lastname: Calvo
authors.forename: Lourdes
authors.initials: L
authors.lastname: Sánchez
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D048014:Clostridium tyrobutyricum / Q000302:isolation & purification*; D008024:Ligands; D008280:Magnetics; D008863:Microspheres; D008892:Milk / Q000382:microbiology*; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D013171:Spores, Bacterial / Q000302:isolation & purification*
chemical_list: D008024:Ligands; D019151:Peptide Library; D010455:Peptides