title: Chromosome-wide analysis of protein binding and modifications.
abstract: In order to fully understand the functions of a DNA-binding protein it is necessary to identify all of its binding sites in chromosomes and assess the role of each site in the overall biological function of the factor. An approach ChIP-on-Chip which combines the chromatin immunoprecipitation technique with chromosomal DNA microarray analysis, has proven to be a powerful means for the chromosome-wide identification of protein binding sites. This approach can also be used to characterize chromosome-wide variations in patterns of post-translational protein modifications, for example histone modifications. This chapter presents methodologies for the ChIP-on-Chip analysis, using as an example the identification of chromosome-wide binding sites for the TATA-binding protein in mitotic cells.
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Sarge
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xing
authors.forename: Ok-Kyong
authors.initials: OK
authors.lastname: Park-Sarge
mesh_terms: D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D002877:Chromosomes, Human; D017931:DNA Primers; D005456:Fluorescent Dyes; D006801:Humans; D011485:Protein Binding
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes