Pyranose dehydrogenases: biochemical features and perspectives of technological applications.
Pyranose dehydrogenase is a fungal flavin-dependent sugar oxidoreductase which is structurally and catalytically related to fungal pyranose oxidase and cellobiose dehydrogenase and probably fulfills similar biological functions in lignocellulose breakdown. It is a monomeric secretory glycoprotein and is limited to a rather small group of litter-decomposing basidiomycetes. Compared with pyranose oxidase, it displays broader substrate specificity and a variable regioselectivity and is unable to utilize oxygen as electron acceptor using substituted benzoquinones and (organo) metallic ions instead. Depending on the structure of the sugar in pyranose form (mono/di/oligosaccharide or glycoside) and the enzyme source, selective monooxidations at C-1, C-2, C-3, or dioxidations at C-2,3 or C-3,4 of the molecule to the corresponding aldonolactones (C-1), or (di)dehydrosugars (aldos(di)uloses) can be performed. These features make pyranose dehydrogenase a promising and versatile biocatalyst for production of highly reactive, sometimes unique, di- and tri-carbonyl sugar derivatives that may serve as interesting chiral intermediates for the synthesis of rare sugars, novel drugs, and fine chemicals.
Peterbauer
Clemens K
CK
Department of Food Sciences and Technology, BOKU-University of Natural Resources and Applied Life Sciences, Vienna, Austria. clemens.peterbauer@boku.ac.at
Volc
Jindrich
J
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002384:Catalysis; D004563:Electrochemistry; D005658:Fungi / Q000201:enzymology / Q000378:metabolism; D010802:Phylogeny; D013379:Substrate Specificity
D002241:Carbohydrates; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase