title: Transplanted bone morphogenetic protein/poly(lactic-co-glycolic acid) delayed-release microcysts combined with rat micromorselized bone and collagen for bone tissue engineering.
abstract: This study was designed to optimize the preparation of delayed-release microcysts containing bone morphogenetic protein 2 (BMP-2) combined with poly(lactic-co-glycolic acid) (PLGA) and to investigate their osteogenic properties when combined with rat autologous micromorselized bone and collagen. Rat autologous micromorselized bone, collagen and BMP-2/PLGA delayed-release microcysts were implanted in various combinations into the rat gluteus maximus muscle sack model. The following post-operative measurements were made: general observations of the implant site, histological observations, osteogenesis measurements and alkaline phosphatase activity. Autologous micromorselized bone combined with collagen and BMP-2/PLGA delayed-release microcysts demonstrated significantly superior osteogenic properties than any of the other combinations of these three components. These findings suggest that micromorselized bone combined with collagen and BMP-2/PLGA delayed-release microcysts could reduce the quantity of BMP-2 and autologous bone required for these procedures, making their use feasible in human bone restoration.
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ji
authors.forename: G P
authors.initials: GP
authors.lastname: Xu
authors.forename: J L
authors.initials: JL
authors.lastname: Yan
authors.forename: S H
authors.initials: SH
authors.lastname: Pan
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D018786:Bone Substitutes / Q000008:administration & dosage*; D016025:Bone Transplantation / Q000473:pathology / Q000502:physiology; D003094:Collagen / Q000008:administration & dosage*; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D006801:Humans; D019344:Lactic Acid; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage; D023822:Tissue Engineering / Q000379:methods*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D003692:Delayed-Action Preparations; D011994:Recombinant Proteins; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen