title: [Determination of six active components in three species of genus Swertia by HPLC multiwavelength with detection].
abstract: OBJECTIVE
  To develop an HPLC method for the quantification of six active components in three species (Swertia davidi, S. nervosa and S. mussotii) .
  
  
  METHODS
  The determination was performed on a Hypersil BDS colunm (4. 6 mm x 200 mm, 5 microm). Acetonitrile and 0.5% phosphoric acid solution were used as the mobile phases with a gradient elution. The flow rate was 1.0 mL x min(-1). The UV detection wavelength was at 240, 274, 325 and 334 nm. The column oven temperature was at 25 degrees C.
  
  
  RESULTS
  Six components were separated commendably in 60 minutes. The calibration curves of swertiamarin, gentiopicroside, norswertianolin, swertianolin, demethylbellidifolin and bellidifolin were in good linearity over the range of 0.520-20.8, 0.202-8.06, 0.107-4.28, 0.097-3.86, 0.094-3.77, 0.101-4.02 microg, respectively (r = 0.999 9). The average recoveries were 98.7%, 98.1%, 98.3%, 98.8%, 98.1% and 98.6%, respectively, and the RSD were less than 3.0% (n = 6).
  
  
  CONCLUSIONS
  The method is accurate,simple and reproducible, and can be used to control the quality of Swertia.
authors.affiliation: School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
authors.forename: Kangping
authors.initials: K
authors.lastname: Xu
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.forename: Fushuang
authors.initials: F
authors.lastname: Li
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Guoru
authors.initials: G
authors.lastname: Liu
authors.forename: Jianbing
authors.initials: J
authors.lastname: Tan
authors.forename: Guishan
authors.initials: G
authors.lastname: Tan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005960:Glucosides / Q000032:analysis; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis; D011753:Pyrones / Q000032:analysis; D029967:Swertia / Q000737:chemistry*; D044004:Xanthones / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011753:Pyrones; D044004:Xanthones; C012997:gentiopicroside; C013270:swertiamarin; C056208:norswertianolin