Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin.
The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rha-(1-->, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-L-Rha-(1-->, which was identified in a previous study.
Konishi
Tomoyuki
T
Forestry and Forest Products Research Institute, 1, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan.
Taguchi
Fumiko
F
Iwaki
Masako
M
Ohnishi-Kameyama
Mayumi
M
Yamamoto
Masanobu
M
Maeda
Ikuko
I
Nishida
Yoshihiro
Y
Ichinose
Yuki
Y
Yoshida
Mitsuru
M
Ishii
Tadashi
T
D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D044224:Pseudomonas syringae / Q000737:chemistry*
D009844:Oligosaccharides