title: Absence of ZnuABC-mediated zinc uptake affects virulence-associated phenotypes of uropathogenic Escherichia coli CFT073 under Zn(II)-depleted conditions.
abstract: In an effort to uncover the role of the high-affinity Zn(II) uptake system in uropathogenic Escherichia coli CFT073, we deleted the znuB gene, which encodes for the transmembrane component of the ZnuABC transporter system. The null mutant for znuB did not grow on minimal medium unless supplemented with excess Zn(II) (50 muM ZnCl(2)). In contrast, the E. coli K-12 DeltaznuB cell line grew well on minimal medium that was not supplemented with Zn(II). The DeltaznuB mutant was significantly deficient in the formation of biofilm under static conditions and also showed a substantially reduced migration front of swarm cells. Because motility and biofilm formation are important for E. coli CFT073 pathogenicity, we propose that the high-affinity Zn(II) uptake system may contribute to the virulence of this pathogen in the urinary tract.
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
authors.forename: Thusitha S
authors.initials: TS
authors.lastname: Gunasekera
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Herre
authors.forename: Michael W
authors.initials: MW
authors.lastname: Crowder
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D001692:Biological Transport; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010641:Phenotype; D056689:Uropathogenic Escherichia coli / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence; D015032:Zinc / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; C546032:ZnuB protein, E coli; D015032:Zinc