title: Enhanced contact hypersensitivity by delayed T-helper 2 response in BALB/c mice.
abstract: T-helper (Th) 1/Th2 balance determines the direction of contact hypersensitivity (CHS). To clarify the immunopathogenesis of contact dermatitis, 2,4-dinitrofluorobenzene (DNFB)-induced CHS reaction was compared between the BALB/c and C57BL/6 mice. The two strains were sensitized with DNFB systemically and challenged with DNFB locally. The CHS reaction in BALB/c mice was intense compared with that in C57BL/6 mice at 24 and 48 hours post-DNFB challenge. The dermal lesions were characterized by infiltration of lymphocytes, eosinophils, neutrophils, and macrophages including CD4+ and CD8+ T cells, and interleukin (IL)-4-producing(+) and interferon (IFN)-gamma+ cells in BALB/c mice. In C57BL/6 mice, the composition of inflammatory cells was same as those in BALB/c mice except for eosinophils, CD4+ T cells, and IL-4+ cells. There was no increase in the number of mast cells in the two strains. Local and systemic productions of IL-4 and IFN-gamma in BALB/c mice were higher than those in C57BL/6 mice. Although blood IgE values increased in BALB/c mice, but not in C57BL/6 mice, at 48 hours postchallenge, its value was low. The delayed Th2-like response together with Th1-like response in BALB/c mice may induce strong CHS reaction compared with C57BL/6 mice, which may dominantly develop Th1-like reaction. Moreover, mast cell and IgE do not appear to be involved in delayed CHS.
authors.affiliation: Laboratory of Veterinary Pathology, Yamaguchi University, 1677-1 Yoshida,Yamaguchi 753-8515, Japan. hayasi@yamaguchi-u.ac.jp
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Hayashi
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hara
authors.forename: Keiko
authors.initials: K
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D003877:Dermatitis, Contact / Q000097:blood / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology*; D004139:Dinitrofluorobenzene / Q000008:administration & dosage / Q000276:immunology*; D018450:Disease Progression; D004804:Eosinophils / Q000276:immunology / Q000473:pathology; D006968:Hypersensitivity, Delayed / Q000097:blood / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology*; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene