MALDI-TOF MS-based drug susceptibility testing of pathogens: the example of Candida albicans and fluconazole.
MALDI-TOF MS can be used for the identification of microorganism species. We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The method was accurate, and reliable, and showed full agreement with the Clinical Laboratory Standards Institute's reference method. This proof-of-concept demonstration highlights the potential for this approach to test other pathogens.
Marinach
Carine
C
INSERM, UMRS 945 Paris, France.
Alanio
Alexandre
A
Palous
Martine
M
Kwasek
Stéphanie
S
Fekkar
Arnaud
A
Brossas
Jean-Yves
JY
Brun
Sophie
S
Snounou
Georges
G
Hennequin
Christophe
C
Sanglard
Dominique
D
Datry
Annick
A
Golmard
Jean-Louis
JL
Mazier
Dominique
D
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D015725:Fluconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D000935:Antifungal Agents; D015725:Fluconazole