title: Multimeric bivalent immunogens from recombinant tetanus toxin HC fragment, synthetic hexasaccharides, and a glycopeptide adjuvant.
abstract: Using recombinant tetanus toxin H(C) fragment (rTT-H(C)) as carrier, we prepared multimeric bivalent immunogens featuring the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Ogawa, in combination with either the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Inaba, or a synthetic disaccharide tetrapeptide peptidoglycan fragment as adjuvant. The conjugation reaction was effected by squaric acid chemistry and monitored in virtually real time by SELDI-TOF MS. In this way, we could prepare well-defined immunogens with predictable carbohydrate-carrier ratio, whose molecular mass and the amount of each saccharide attached could be independently determined. The ability to prepare such neoglycoconjugates opens unprecedented possibilities for preparation of conjugate vaccines for bacterial diseases from synthetic carbohydrates.
authors.affiliation: NIDDK, LBC, National Institutes of Health, Bethesda, MD 20892-0815, USA.
authors.forename: Aileen F G
authors.initials: AF
authors.lastname: Bongat
authors.forename: Rina
authors.initials: R
authors.lastname: Saksena
authors.forename: Roberto
authors.initials: R
authors.lastname: Adamo
authors.forename: Yukari
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Zenyu
authors.initials: Z
authors.lastname: Shiokawa
authors.forename: Dwight C
authors.initials: DC
authors.lastname: Peterson
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukase
authors.forename: Willie F
authors.initials: WF
authors.lastname: Vann
authors.forename: Pavol
authors.initials: P
authors.lastname: Kovác
mesh_terms: D000276:Adjuvants, Immunologic / Q000138:chemical synthesis*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry; D013744:Tetanus Toxin / Q000737:chemistry / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D006001:Glycoconjugates; D006020:Glycopeptides; D009844:Oligosaccharides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic; C040635:tetanus toxin fragment C