In vitro antiherpetic and antirotaviral activities of a sulfate prepared from Mimosa scabrella galactomannan.
A chemically sulfated galactomannan (BRS) from seeds of Mimosa scabrella had in vitro antiviral activity against Herpes simplex virus 1 (HSV-1), but not against Simian rotavirus A/SA11 (SiRV-A/SA11). It was examined by (13)C NMR spectroscopy, which showed the sulfate groups to be mainly at C-6 of galactose residues. BRS had a selective inhibition against HSV-1 during its attachment step, having an IC(50) lower than 2.5microg/ml, determined by plaque reduction, and a selectivity index of greater than 181, suggesting that the antiviral effect is likely due to interactions between the virus and BRS, being influenced its overall surface charge.
Chrestani
Francielli
F
Laboratório de Microbiologia Yasuyoshi Hayashi, Departamento de Patologia Básica, Setor de Ciências Biológicas, Centro Politécnico, Universidade Federal do Paraná, Jardim das Américas, CEP: 81531-990, Curitiba, Paraná, Brazil.
Sierakowski
Maria Rita
MR
de Andrade Uchoa
Daniel Esdras
DE
Nozawa
Carlos
C
Sassaki
Guilherme Lanzi
GL
Gorin
Philip Albert James
PA
Ono
Lucy
L
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002522:Chlorocebus aethiops; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005690:Galactose / Q000737:chemistry / Q000031:analogs & derivatives; D018259:Herpesvirus 1, Human / Q000187:drug effects; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry*; D031298:Mimosa / Q000378:metabolism*; D008956:Models, Chemical; D010936:Plant Extracts / Q000494:pharmacology*; D012401:Rotavirus / Q000187:drug effects; D013055:Spectrophotometry, Infrared / Q000379:methods; D013431:Sulfates / Q000494:pharmacology*; D014709:Vero Cells
D000998:Antiviral Agents; D008351:Mannans; D010936:Plant Extracts; D013431:Sulfates; C012990:galactomannan; D005690:Galactose