DNA supercoiling regulates the stress-inducible expression of genes in the cyanobacterium Synechocystis.
Changes in the supercoiling of genomic DNA play an important role in the regulation of gene expression. We compared the genome-wide expression of genes in cells of the cyanobacterium Synechocystis sp. PCC 6803 when they were subjected to salt, cold, and heat stress, in the presence of novobiocin, an inhibitor of DNA gyrase, and in its absence. The analysis revealed that the expression of a large number of stress-inducible genes depends on the extent of genomic DNA supercoiling. The function of the two-component regulatory systems, which are known as sensors and transducers of salt, cold, and heat stress, depends on, and might be controlled by, the degree of supercoiling of the genomic DNA. These results suggest that stress-induced changes in superhelicity of genomic DNA provide an important permissive background for successful acclimatization of cyanobacterial cells to stress conditions.
Prakash
Jogadhenu S S
JS
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Gachibowli, Hyderbad 500 046, Andhra Pradesh, India.
Sinetova
Maria
M
Zorina
Anna
A
Kupriyanova
Elena
E
Suzuki
Iwane
I
Murata
Norio
N
Los
Dmitry A
DA
D015152:Blotting, Northern; D016000:Cluster Analysis; D004278:DNA, Superhelical / Q000235:genetics / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D009675:Novobiocin / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D012965:Sodium Chloride / Q000737:chemistry; D013312:Stress, Physiological / Q000502:physiology*; D046939:Synechocystis / Q000235:genetics / Q000502:physiology*; D013696:Temperature
D004278:DNA, Superhelical; D004791:Enzyme Inhibitors; D009675:Novobiocin; D012965:Sodium Chloride