Effect of albumin concentration and serum matrix on ischemia-modified albumin.
OBJECTIVE
There is concern that ischemia-modified albumin (IMA) levels measured by albumin cobalt binding (ACB) assay reflect mainly albumin concentrations rather than myocardial ischemia.


METHODS
Serum matrix and proteins were separated from a serum pool by a membrane filter. Two series of pools with albumin concentrations of 10, 20, 30, 40, 50, and 60 g/L were prepared either with human albumin or serum protein fraction. IMA values of these pools were measured in quintiplicate.


RESULTS
There was a strong negative correlation between IMA and albumin levels in both pools. IMA change corresponding to each 10 g/L difference in albumin concentration was 37% and 48% in these pools.


CONCLUSIONS
ACB assay reflects albumin concentrations rather than IMA. Primary predictor of IMA in serum matrix is albumin concentration.
Hakligör
Aylin
A
Department of Clinical Biochemistry, Ankara Training and Research Hospital, Ulucanlar Caddesi, Cebeci, Ankara 06340, Turkey.
Kösem
Arzu
A
Seneş
Mehmet
M
Yücel
Doğan
D
D001681:Biological Assay / Q000379:methods; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism; D006801:Humans; D017202:Myocardial Ischemia / Q000097:blood*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism
D001798:Blood Proteins; D012709:Serum Albumin; D003035:Cobalt