title: A COL2A1 gene polymorphism is related with advanced stages of osteoarthritis of the knee in Mexican Mestizo population.
abstract: Primary osteoarthritis (OA) is a multifactorial disease with several genetics factors involved. The COL2A1 gene is of particular interest because it encodes for the most abundant protein in articular cartilage. The aim was to evaluate the association of COL2A1 gene polymorphism with OA of the knee in Mexican Mestizo patients. A case-control study was conducted; cases comprised patients with a radiologic scoring > or = 2 and controls with a radiologic scoring <2. DNA was extracted from a peripheral blood sample, the polymorphic site of the COL2A1 gene was submitted to polymerase chain reaction (PCR), and the products were digested using PvuII restriction enzyme. For statistical analysis, a non-conditional logistic regression was developed. There were no associations among alleles in the overall sample, nevertheless, a significant association was found with p (Pp/pp) allele and OA of the knee grade 4 [odds ratio (OR), 95% confidence interval (CI 95%) 4.1 (1.2-14.6)] adjusted by gender, age, and body mass index (BMI). These results suggest an association of a COL2A1 gene polymorphism with advanced stages of OA of the knee in Mexican Mestizo population.
authors.affiliation: Servicio de Genética, Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, Deleg. Tlalpan, 14889, México, D.F., Mexico.
authors.forename: Arturo
authors.initials: A
authors.lastname: Gálvez-Rosas
authors.forename: Celia
authors.initials: C
authors.lastname: González-Huerta
authors.forename: Verónica Marusa
authors.initials: VM
authors.lastname: Borgonio-Cuadra
authors.forename: Carolina
authors.initials: C
authors.lastname: Duarte-Salazár
authors.forename: Lorena
authors.initials: L
authors.lastname: Lara-Alvarado
authors.forename: María
authors.initials: M
authors.lastname: de los Angeles Soria-Bastida
authors.forename: Socorro
authors.initials: S
authors.lastname: Cortés-González
authors.forename: Eva
authors.initials: E
authors.lastname: Ramón-Gallegos
authors.forename: Antonio
authors.initials: A
authors.lastname: Miranda-Duarte
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D024043:Collagen Type II / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology / Q000208:ethnology; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000208:ethnology / Q000235:genetics* / Q000276:immunology*; D011110:Polymorphism, Genetic / Q000235:genetics*; D055815:Young Adult
chemical_list: C499177:COL2A1 protein, human; D024043:Collagen Type II