title: Discovery of new natural products by intact-cell mass spectrometry and LC-SPE-NMR: malbranpyrroles, novel polyketides from thermophilic fungus Malbranchea sulfurea.
abstract: Six photosensitive polyketides, malbranpyrroles A-F, were discovered from the thermophilic fungus Malbranchea sulfurea by using intact-cell desorption/ionization on silicon mass (ICD-MS) and LC-SPE-NMR. These two strategies facilitate the searching and structural determination of unstable natural products. The ICD-MS indicated that only brown hyphae of M. sulfurea can produce malbranpyrroles. The biosynthetic pathway of malbranpyrroles was evidenced by 13C isotope precursors and amino acid feeding experiments. The cytotoxicity data revealed that the conformation of the conjugated system in malbranpyrroles does not affect cytotoxic potency against cancer cell lines. In addition, the chlorine atom was shown to be the pharmacophore for cytotoxicity.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Yang
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Liao
authors.forename: Wan-Yun
authors.initials: WY
authors.lastname: Liu
authors.forename: Chih-Chuang
authors.initials: CC
authors.lastname: Liaw
authors.forename: Chia-Ning
authors.initials: CN
authors.lastname: Shen
authors.forename: Zih-You
authors.initials: ZY
authors.lastname: Huang
authors.forename: Shih-Hsiung
authors.initials: SH
authors.lastname: Wu
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000737:chemistry*; D006801:Humans; D018942:Macrolides / Q000737:chemistry* / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D011758:Pyrroles / Q000737:chemistry* / Q000633:toxicity; D052616:Solid Phase Extraction
chemical_list: D001688:Biological Products; D018942:Macrolides; D011758:Pyrroles