Upc2p-associated differential protein expression in Candida albicans.
The gain-of-function mutation G648D in UPC2 causes ERG11 up-regulation and increased fluconazole resistance in Candida albicans. In this study, we performed 2-DE and PMF to identify proteomic alterations in an ERG11-overexpressing fluconazole-resistant C. albicans clinical isolate compared with its fluconazole-susceptible parent strain. We identified 23 differentially expressed proteins, and among them, seven became differentially expressed in a C. albicans wild-type strain after the introduction of a UPC2 allele carrying this mutation. These Upc2p-regulated proteins may contribute to fluconazole resistance in C. albicans.
Hoehamer
Christopher F
CF
Departments of Clinical Pharmacy, Pharmaceutical Sciences, and Pediatrics, Colleges of Pharmacy and Medicine, University of Tennessee Health Science Center, Memphis, TN, USA.
Cummings
Edwin D
ED
Hilliard
George M
GM
Morschhäuser
Joachim
J
David Rogers
Phillip
P
D002176:Candida albicans / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020543:Proteome / Q000032:analysis*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D005656:Fungal Proteins; D020543:Proteome; D015534:Trans-Activators; D015725:Fluconazole