Genome-wide analysis for protein-DNA interaction: ChIP-chip.
Chromatin immunoprecipitation (ChIP) is a well-established procedure for protein-DNA interaction research. ChIP-chip, combining chromatin immunoprecipitation (ChIP) and microarray technology (Chip), enables scientists to survey genome-wide DNA binding sites for a given protein. The ChIP-chip technique has been used to identify transcription factor binding sites, explore epigenomic information and investigate factors in DNA replicate/repairs. Here we describe a protocol for ChIP-chip to study Pituitary Tumor Transforming Gene (PTTG1) in mammalian cells.
Tong
Yunguang
Y
Department of Medicine, Cedars-Sinai Research Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Falk
Jeff
J
D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D016678:Genome; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000737:chemistry*
D017931:DNA Primers; D011506:Proteins; D004247:DNA