Production and biochemical characterization of a type B ferulic acid esterase from Streptomyces ambofaciens.
For the first time, the presence of a ferulic acid esterase (FAE) was demonstrated in Streptomyces ambofaciens. This extracellular enzyme was produced on a range of lignocellulosic substrates. The maximal level of activity was detected in the presence of either destarched wheat bran or oat spelt xylan as the sole carbon source. We found that 1% (m/v) of destarched wheat bran was the optimal concentration to induce its production. With this inducer, no ferulic acid dimers were released from the cell wall by the produced FAE. Interestingly, rape cattle cake (Brassica napus), which does not contain esterified ferulic acid, was also shown to induce the production of the FAE from S. ambofaciens. The FAE was partially purified from the culture supernatant. The purified enzyme was optimally active at pH 7 and 40 degrees C. The substrate specificity of the FAE from S. ambofaciens was investigated: the highest activity was determined with methyl p-coumarate, methyl ferulate, and methyl cinnamate. Furthermore, the FAE required a certain distance between the benzene ring and the ester bond to be active. According to these biochemical characteristics, the FAE from S. ambofaciens has been classified as a type B FAE.
Kheder
Fadi
F
Laboratoire d'Ingénierie des Biomolécules, Nancy-Université, B.P. 172 F-54505 Vandoeuvre les Nancy, France.
Delaunay
Stéphane
S
Abo-Chameh
Ghassan
G
Paris
Cédric
C
Muniglia
Lionel
L
Girardin
Michel
M
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D004795:Enzyme Stability; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity
D001426:Bacterial Proteins; D003373:Coumaric Acids; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase