Systematic evaluation of a tissue-engineered bone for maxillary sinus augmentation in large animal canine model.
The objective of this study is to systematically evaluate the effects of a tissue-engineered bone complex for maxillary sinus augmentation in a canine model. Twelve sinus floor augmentation surgeries in 6 animals were performed bilaterally and randomly repaired with the following 3 groups of grafts: group A consisted of tissue-engineered osteoblasts/beta-TCP complex (n=4); group B consisted of beta-TCP alone (n=4); group C consisted of autogenous bone obtained from iliac crest as a positive control (n=4). All dogs had uneventful healings following the surgery. Sequential polychrome fluorescent labeling, maxillofacial CT, microhardness tests, as well as histological and histomorphometric analyses indicated that the tissue-engineered osteoblasts/beta-TCP complex dramatically promoted bone formation and mineralization and maximally maintained the height and volume of elevated maxillary sinus. By comparison, both control groups of beta-TCP or autologous iliac bone showed considerable resorption and replacement by fibrous or fatty tissue. We thus conclude that beta-TCP alone could barely maintain the height and volume of the elevated sinus floor, and that the transplantation of autogenous osteoblasts on beta-TCP could promote earlier bone formation and mineralization, maximally maintain height, volume and increase the compressive strength of augmented maxillary sinus. This tissue engineered bone complex might be a better alternative to autologous bone for the clinical edentulous maxillary sinus augmentation.
Wang
Shaoyi
S
Oral Bioengineering Lab (Oral Tissue Engineering Lab), Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai, China.
Zhang
Zhiyuan
Z
Xia
Lunguo
L
Zhao
Jun
J
Sun
Xiaojuan
X
Zhang
Xiuli
X
Ye
Dongxia
D
Uludağ
Hasan
H
Jiang
Xinquan
X
D000469:Alkaline Phosphatase / Q000378:metabolism; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration / Q000502:physiology; D004285:Dogs; D007150:Immunohistochemistry; D008443:Maxillary Sinus / Q000601:surgery*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D014057:Tomography, X-Ray Computed
D001672:Biocompatible Materials; D015675:Osteocalcin; D000469:Alkaline Phosphatase