title: Use of propyl paraben to control growth and ochratoxin A production by Aspergillus section Nigri species on peanut meal extract agar.
abstract: This study was carried out to determine the efficacy of the phenolic antioxidant propyl paraben (PP) under different interacting water activity (a(W)) and temperature regimes on lag phase, growth rate and Ochratoxin A production by Aspergillus section Nigri strains. In this experiment six Aspergillus section Nigri strains were used. Peanut meal extract agar (PMEA) was prepared at 2%. The a(W) of the medium was adjusted to 0.995, 0.980 and 0.930, PP levels of 1, 5, 10 and 20 mmol/L were added to the basic medium. Plates were inoculated and incubated for 30 days at 18 and 25 degrees C. Lag phase (h) and radial growth rates (mm/day) were calculated. In control treatments, the lag phase increased and the growth rate decreased as a(W) reduced in all assayed strains. At all a(W) levels, when antioxidant concentrations increased the growth rate decreased. At 5, 10 and 20 mmol/L of PP the strains were not able to reach the exponential phase and completely inhibited fungal growth and OTA production regardless of a(W) used in all the evaluated strains.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
authors.forename: Carla
authors.initials: C
authors.lastname: Barberis
authors.forename: Andrea
authors.initials: A
authors.lastname: Astoreca
authors.forename: Guillermina
authors.initials: G
authors.lastname: Fernandez-Juri
authors.forename: Sofía
authors.initials: S
authors.lastname: Chulze
authors.forename: Ana
authors.initials: A
authors.lastname: Dalcero
authors.forename: Carina
authors.initials: C
authors.lastname: Magnoli
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D010367:Arachis / Q000382:microbiology*; D001230:Aspergillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media; D009793:Ochratoxins / Q000096:biosynthesis*; D010226:Parabens / Q000494:pharmacology*; D013696:Temperature; D014867:Water
chemical_list: D000975:Antioxidants; D003470:Culture Media; D009793:Ochratoxins; D010226:Parabens; D014867:Water; C025589:ochratoxin A; C006068:propylparaben