Preparation of an ochre suppressor tRNA recognizing exclusively UAA codon by using the molecular surgery technique.
In order to create an ochre suppressor tRNA which exclusively recognizes UAA codon, we replaced the G34 at the first position of yeast tRNA(Tyr)[GPsiA] anticodon with pseudouridine34 (Psi34) by using the molecular surgery technique. This tRNA(Tyr)[PsiPsiA] recognized only the UAA codon as expectedly, but tRNA(Tyr)[UPsiA] made as a control also behaved similarly. This result may suggest that U34 must be somehow modified to facilitate the wobble-pairing to G at the third position of codon.
Yokogawa
Takashi
T
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan.
Hassan
Hanim Munirah Binti Che
HM
Yokota
Yukiko
Y
Ohno
Satoshi
S
Nishikawa
Kazuya
K
D000926:Anticodon / Q000737:chemistry; D018388:Codon, Terminator / Q000737:chemistry*; D005821:Genetic Techniques; D011560:Pseudouridine / Q000737:chemistry; D012365:RNA, Transfer, Tyr / Q000737:chemistry*
D000926:Anticodon; D018388:Codon, Terminator; D012365:RNA, Transfer, Tyr; D011560:Pseudouridine