Assessment of plasma membrane and chromatin structure of sperm from transgenic and non-transgenic boars.
The aim of this study was to determine the apoptotic changes and chromatin damage in non-transgenic and transgenic boars carrying the human alpha1,2-fucosyltransferase gene. Five ejaculates were collected from six transgenic (TG) and six non-transgenic (NTG) boars. Five ejaculates were collected from six transgenic (TG) and six non-transgenic (NTG) boars both crossbreds of Polish Landrace and Large White. Two fluorescence methods were employed to measure apoptosis: an assay to assess the early changes in sperm membrane integrity using fluorophore YO-PRO-1 and an assay for phosphatidylserine (PS) translocation across the plasma membrane using fluorescein-labeled Annexin-V. The chromatin damage was assessed based on the sperm chromatin structure assay method. No significant differences in the proportion of all detected subpopulations of spermatozoa were found between TG and NTG boars. Similarly, the analysis of the chromatin structure revealed no statistical differences in the sperm chromatin damage between TG and NTG boars. In conclusion, the presence of the human alpha1,2-fucosyltransferase gene in the genome of TG boars did not cause any spermatogenesis process disturbances leading to the increased production of apoptotic spermatozoa. Moreover, the low level of sperm with damaged chromatin in TG boars confirms the high stability of the spermatogenesis process in the TG boars analyzed.
Trzcińska
M
M
Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Balice/Kraków, Poland. mcala@izoo.krakow.pl
Bryła
M
M
Bochenek
M
M
Słomski
R
R
Smorag
Z
Z
D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis; D002462:Cell Membrane / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D005647:Fucosyltransferases / Q000235:genetics; D006801:Humans; D008297:Male; D055101:Semen Analysis; D013094:Spermatozoa / Q000648:ultrastructure*; D013552:Swine / Q000235:genetics*
D002843:Chromatin; D005647:Fucosyltransferases