title: In vitro and in vivo genotoxicity tests on fullerene C60 nanoparticles.
abstract: There are several conflicting reports on the genotoxicity of fullerene C(60) in the literature. To determine the genotoxic potential of C(60) nanoparticles, we prepared stable nano-sized C(60) suspensions using 0.1% carboxymethylcellulose sodium (CMC-Na) or 0.1% Tween 80 aqueous solution. We conducted a bacterial reverse mutation test with Ames Salmonella typhimurium TA98, TA100, TA1535, and TA1537 strains and Escherichia coli strain and a chromosomal aberration test with cultured Chinese hamster CHL/IU cells in the presence and absence of metabolic activation under dark conditions and visible light irradiation using a stable C(60) nanoparticle suspension with CMC-Na. In addition, we performed a bone marrow micronucleus test using a stable C(60) nanoparticle suspension with Tween 80 on ICR mice. C(60) nanoparticles did not show a positive mutagenic response up to the maximum dose of 1000 microg/plate with any tester strain in the bacterial reverse mutation test regardless of metabolic activation and irradiation, although a slight but not significant increase in the number of revertants was observed in TA100 and WP2 uvrA/pKM101. No increase in the incidence of chromosomal aberrations was observed at any C(60) nanoparticle dose regardless of metabolic activation and irradiation in the chromosomal aberration test up to the maximum doses of 100 and 200 microg/mL. In addition, the micronucleus test showed that the in vivo clastogenic ability of the C(60) nanoparticles was negative up to the maximum dose of 88 mg/kg x 2. Therefore, we concluded that the stable and well-characterized C(60) nanoparticles did not have genotoxic ability in the bacterial reverse mutation assay, in vitro chromosome aberration assay, nor in vivo micronucleus assay.
authors.affiliation: Research Institute of Science for Safety and Sustainability, AIST, 16-1, Onogawa, Tsukuba, Ibaraki 305-8569, Japan. n-shinohara@aist.go.jp
authors.forename: Naohide
authors.initials: N
authors.lastname: Shinohara
authors.forename: Kyomu
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Endoh
authors.forename: Junko
authors.initials: J
authors.lastname: Maru
authors.forename: Junko
authors.initials: J
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D016466:CHO Cells; D002266:Carboxymethylcellulose Sodium; D002869:Chromosome Aberrations / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003624:Darkness; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D037741:Fullerenes / Q000633:toxicity*; D008027:Light; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D053758:Nanoparticles / Q000633:toxicity*; D011136:Polysorbates; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013535:Suspensions
chemical_list: D037741:Fullerenes; D009153:Mutagens; D011136:Polysorbates; D013535:Suspensions; D002266:Carboxymethylcellulose Sodium