title: Co-localization of carbonic anhydrase and phosphoenol-pyruvate carboxylase and localization of pyruvate kinase in roots and hypocotyls of etiolated Glycine max seedlings.
abstract: We investigated the presence of carbonic anhydrase in root and hypocotyl of etiolated soybean using enzymatic, histochemical, immunohistochemical and in situ hybridization approaches. In parallel, we used in situ hybridization and immunolocalization to determine the expression pattern and localization of phosphoenolpyruvate carboxylase. Their co-localization in the root tip as well as in the central cylinder, suggests that a large fraction of the CO(2) may be re-introduced into C4 compounds. GmPK3 expression, coding for a cytoplasmic isoform of pyruvate kinase, was detected in all different root cell types, suggesting that both phosphoenolpyruvate-utilizing enzymes are involved in phosphoenolpyruvate metabolism in etiolated soybean roots; a case indicative of the necessary flexibility plant metabolism has to adopt in order to compensate various physiological conditions.
authors.affiliation: Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Dimou
authors.affiliation: Institute of Biology, Splaiul Independentei 296, Bucharest 060031, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Paunescu
authors.affiliation: Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Aivalakis
authors.affiliation: Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Flemetakis
authors.affiliation: Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Katinakis
mesh_terms: D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D018546:Hypocotyl / Q000201:enzymology* / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology* / Q000378:metabolism; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011770:Pyruvate Kinase; D010730:Phosphoenolpyruvate Carboxylase; D002256:Carbonic Anhydrases