Characterization and classification of matrix effects in biological samples analyses.
An exhaustive classification of matrix effects occurring when a sample preparation is performed prior to liquid-chromatography coupled to mass spectrometry (LC-MS) analyses was proposed. A total of eight different situations were identified allowing the recognition of the matrix effect typology via the calculation of four recovery values. A set of 198 compounds was used to evaluate matrix effects after solid phase extraction (SPE) from plasma or urine samples prior to LC-ESI-MS analysis. Matrix effect identification was achieved for all compounds and classified through an organization chart. Only 17% of the tested compounds did not present significant matrix effects.
Marchi
Ivano
I
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
Viette
Véronique
V
Badoud
Flavia
F
Fathi
Marc
M
Saugy
Martial
M
Rudaz
Serge
S
Veuthey
Jean-Luc
JL
D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000652:urine*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
D004364:Pharmaceutical Preparations