title: Mouse orthotopic lung cancer model induced by PC14PE6.
abstract: OBJECTIVE
  This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells.
  
  
  METHODS
  Three cell doses (0.5x10(6); 1x10(6); 2x10(6)) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells.
  
  
  RESULTS
  The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average life span of the three groups after inoculation was 14 days in the 2x10(6) cells inoculum group; 25 days in the 1x10(6) cells inoculum group; and 32 days in the 0.5x10(6) cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax.
  
  
  CONCLUSIONS
  This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis.
authors.affiliation: Medical Nano Element Development Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Zheng Yun
authors.initials: ZY
authors.lastname: Cui
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Ahn
authors.forename: Jee Yun
authors.initials: JY
authors.lastname: Lee
authors.forename: Won Seog
authors.initials: WS
authors.lastname: Kim
authors.forename: Ho Yeong
authors.initials: HY
authors.lastname: Lim
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Jeon
authors.forename: Soo Won
authors.initials: SW
authors.lastname: Suh
authors.forename: Jin Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Won Ho
authors.initials: WH
authors.lastname: Kong
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Kang
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Nam
authors.forename: Keunchil
authors.initials: K
authors.lastname: Park