title: Isolation of a Methylobacillus sp. that degrades microcystin toxins associated with cyanobacteria.
abstract: Sludge from cyanobacteria-salvaged yard in Meiliang Bay, Lake Taihu in Wuxi, China was cultured and acclimated by inoculating microcystins (MCs) extract. Strain J10 was isolated by degrading the MC-RR and MC-LR and was identified as Methylobacillus sp. Further research showed that both MC-LR and MC-RR could be completely degraded at 17h after inoculation of J10, and the degradation probably was mediated by oxygen. Different enzymes, oxygen-dependent as well as oxygen-independent, with MC-degrading activity were found in the different fractions of J10 culture. However, the enzymes mainly responsible for MC degradation by J10 were oxygen-dependent and were probably bound to cell wall or outside the cytoplasmic membrane.
authors.affiliation: School of Food, Henan Institute of Science and Technology, Xinxiang 453003, China.
authors.forename: Liang Bin
authors.initials: LB
authors.lastname: Hu
authors.forename: Jing Dong
authors.initials: JD
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Yu Fen
authors.initials: YF
authors.lastname: Yin
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Zhi Qi
authors.initials: ZQ
authors.lastname: Shi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry* / Q000187:drug effects; D020631:Methylobacillus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D052998:Microcystins / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010802:Phylogeny; D013997:Time Factors
chemical_list: D052998:Microcystins; C078588:microcystin; D010100:Oxygen