title: Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene.
abstract: Ethanol was a major byproduct of 2,3-butanediol (2,3-BD) fermentation by Klebsiella oxytoca ME-UD-3. In order to achieve a high efficiency of 2,3-BD production, K. oxytoca mutants deficient in ethanol formation were successfully constructed by replace the aldA gene coding for aldehyde dehydrogenase with a tetracycline resistance cassette. The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production. The carbon flux to 2,3-BD was enhanced by eliminating the byproducing ethanol and at the same time reducing the accumulation of another byproduct acetoin. At last, by fed-batch culturing of the mutant, the final 2,3-BD titer up to 130 g/l with the productivity of 1.63 g/l.h and the 2,3-BD yield relative to glucose of 0.48 g/g was obtained.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhu
authors.forename: Lu-Jing
authors.initials: LJ
authors.lastname: Ren
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Nie
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D055785:Gene Knockdown Techniques; D005818:Genetic Engineering; D041121:Klebsiella oxytoca / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+)