title: Induction of JAM-A during differentiation of human THP-1 dendritic cells.
abstract: Junctional adhesion molecule (JAM)-A is not only localized at tight junctions of endothelial and epithelial cells but is also expressed on circulating leukocytes and dendritic cells (DCs). In the present study, to investigate the regulation of JAM-A in DCs, mature DCs were differentiated from the human monocytic cell THP-1 by treatment with IL-4, GM-CSF, TNF-alpha, and ionomycin, and some cells were pretreated with the PPAR-gamma agonists. In the THP-1 monocytes, mRNAs of tight junction molecules, occludin, tricellulin, JAM-A, ZO-1, ZO-2 and claudin-4, -7, -8, and -9 were detected by RT-PCR. In mature DCs that had elongated dendrites, mRNA and protein of JAM-A were significantly increased compared to the monocytes. PPAR-gamma agonists prevented the elongation of dentrites but not upregulation of JAM-A in mature DCs. These findings indicated that the induction of JAM-A occurred during differentiation of human THP-1 DCs and was independent of PPAR-gamma and the p38 MAPK pathway.
authors.affiliation: Department of Otolaryngology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ogasawara
authors.forename: Takashi
authors.initials: T
authors.lastname: Kojima
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Go
authors.forename: Jun
authors.initials: J
authors.lastname: Fuchimoto
authors.forename: Ryuta
authors.initials: R
authors.lastname: Kamekura
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Koizumi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohkuni
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Masaki
authors.forename: Masaki
authors.initials: M
authors.lastname: Murata
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Shingo
authors.initials: S
authors.lastname: Ichimiya
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Himi
authors.forename: Norimasa
authors.initials: N
authors.lastname: Sawada
mesh_terms: D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002454:Cell Differentiation; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D047495:PPAR gamma / Q000819:agonists / Q000378:metabolism; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D007136:Immunoglobulins; D047495:PPAR gamma; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D015759:Ionomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D048051:p38 Mitogen-Activated Protein Kinases