title: [Heterotopic complex membrane induces bone formation].
abstract: OBJECTIVE
  The composite membrane was made by recombinant human bone morphogenetic protein-2 (rhBMP-2), collagen (Co), polylactic-co-glycolic acid (PLGA). To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane.
  
  
  METHODS
  48 male Kunming adult mice were divided into two groups randomly: Co/PLGA complex membrane group and rhBMP-2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination.
  
  
  RESULTS
  Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft X-ray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane.
  
  
  CONCLUSIONS
  Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.
authors.affiliation: Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China.
authors.forename: Chong-tao
authors.initials: CT
authors.lastname: Lin
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Li
authors.forename: Jia-qing
authors.initials: JQ
authors.lastname: Yan
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D003094:Collagen; D006016:Glycolates; D019344:Lactic Acid; D008297:Male; D051379:Mice; D010012:Osteogenesis; D011091:Polyesters; D011100:Polyglycolic Acid; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta
chemical_list: D055396:Bone Morphogenetic Protein 2; D006016:Glycolates; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; C031149:glycolic acid; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen