title: Expression, purification and characterization of UvrD2 helicase from Mycobacterium tuberculosis.
abstract: Helicases appear to be important for genome stability of dormant Mycobacterium tuberculosis responsible for latent tuberculosis infection and big proportion of active disease cases caused by reactivation. It was demonstrated that in both M. tuberculosis and Mycobacterium smegmatis, a helicase termed UvrD2 is essential for bacterial growth making it a promising target to fight tuberculosis. In many cases expression of soluble and active mycobacterial proteins in Escherichia coli is a complicated issue. In this work we for the first time report a non-trivial expression procedure in E. coli, leading to soluble UvrD2 from M. tuberculosis which possesses DNA-dependent ATP-ase activity.
authors.affiliation: Dipartimento di Scienze Chimiche, Alimentari, Farmaceutiche e Farmacologiche, Università del Piemonte Orientale A. Avogadro, Novara, Italy.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Kazarian
authors.forename: Camilla
authors.initials: C
authors.lastname: Cassani
authors.forename: Menico
authors.initials: M
authors.lastname: Rizzi
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D055985:Latent Tuberculosis / Q000201:enzymology / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D004247:DNA; D000251:Adenosine Triphosphatases; D004265:DNA Helicases