Enhanced enrichment and detection of thermotolerant Campylobacter species from water using the Portable Microbe Enrichment Unit and real-time PCR.
An enhanced enrichment using the Portable Microbe Enrichment Unit (PMEU) with the microaerobic bubbling of broths was applied for the detection of thermotolerant Campylobacter species from water. This PMEU enrichment was compared with the conventional static enrichment of the international standard ISO 17995:2005. In addition, Campylobacter detection after enrichment using a real-time PCR detection was compared with colony counts. The tests with stressed Campylobacter jejuni cells in drinking water indicated that the PMEU enrichment yielded a significantly higher number of Campylobacter cells in the Bolton broth compared with the conventional static incubation. Application of the real-time PCR technique shortened the Campylobacter detection time. This combination of method modifications can be used for Campylobacter detection from water and adds methodological repertoire for the rapid survey and management of waterborne outbreaks.
Pitkänen
Tarja
T
National Institute for Health and Welfare, Department of Environmental Health, Water and Health Unit, PO Box 95, FI-70701 Kuopio, Finland. tarja.pitkanen@thl.fi
Bräcker
Juliane
J
Miettinen
Ilkka T
IT
Heitto
Anneli
A
Pesola
Jouni
J
Hakalehto
Elias
E
D001431:Bacteriological Techniques / Q000379:methods*; D002167:Campylobacter / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D005618:Fresh Water / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature