title: Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.
abstract: SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, Narashino, Chiba 275-0016, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Haraguchi
authors.forename: Kanako
authors.initials: K
authors.lastname: Kuwasako
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Muto
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Bessho
authors.forename: Madoka
authors.initials: M
authors.lastname: Nishimoto
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Akio
authors.initials: A
authors.lastname: Kanai
authors.forename: Gota
authors.initials: G
authors.lastname: Kawai
authors.forename: Taiichi
authors.initials: T
authors.lastname: Sakamoto
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry; D052156:SELEX Aptamer Technique; D017423:Sequence Analysis, RNA; D018271:Signal Recognition Particle / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D018271:Signal Recognition Particle; D012313:RNA