title: Protein expression profile using two-dimensional gel analysis in squamous cervical cancer patients.
abstract: OBJECTIVE
  Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two- dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI- TOF) mass spectrometer.
  
  
  METHODS
  Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3 approximately 10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D software. The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases.
  
  
  RESULTS
  A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins were down-regulated. Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, alpha-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study.
  
  
  CONCLUSIONS
  2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC.
authors.affiliation: Cancer Research Institute, The Catholic University of Korea, Seoul, Korea.
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Bae
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Min
authors.forename: Guo Hua
authors.initials: GH
authors.lastname: Ding
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kwak
authors.forename: Young-Lae
authors.initials: YL
authors.lastname: Cho
authors.forename: Kye-Hyun
authors.initials: KH
authors.lastname: Nam
authors.forename: Choong Hak
authors.initials: CH
authors.lastname: Park
authors.forename: Yong-Wan
authors.initials: YW
authors.lastname: Kim
authors.forename: Chong-Kook
authors.initials: CK
authors.lastname: Kim
authors.forename: Byoung-Don
authors.initials: BD
authors.lastname: Han
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: Do Kang
authors.initials: DK
authors.lastname: Kim
authors.forename: Woong-Shick
authors.initials: WS
authors.lastname: Ahn