title: Flow cytometric analysis of benthic prokaryotes attached to sediment particles.
abstract: Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity.
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, Monterotondo, Rome, Italy. amalfitano@irsa.cnr.it
authors.forename: Stefano
authors.initials: S
authors.lastname: Amalfitano
authors.forename: Stefano Fazi Alberto
authors.initials: SF
authors.lastname: Puddu
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D019015:Geologic Sediments / Q000382:microbiology*; D009696:Nucleic Acids / Q000032:analysis / Q000235:genetics; D011387:Prokaryotic Cells / Q000737:chemistry / Q000145:classification*; D045483:Rivers; D013194:Staining and Labeling / Q000379:methods
chemical_list: D009696:Nucleic Acids