title: (1)H, (13)C and (15)N assignments of a camelid nanobody directed against human alpha-synuclein.
abstract: Nanobodies are single chain antibodies that are uniquely produced in Camelidae, e.g. camels and llamas. They have the desirable features of small sizes (Mw < 14 kDa) and high affinities against antigens (Kd approximately nM), making them ideal as structural probes for biomedically relevant motifs both in vitro and in vivo. We have previously shown that nanobody binding to amyloidogenic human lysozyme variants can effectively inhibit their aggregation, the process that is at the origin of systemic amyloid disease. Here we report the NMR assignments of a new nanobody, termed NbSyn2, which recognises the C-terminus of the intrinsically disordered protein, human alpha-synuclein (aS), whose aberrant self-association is implicated in Parkinson's disease.
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge, UK.
authors.forename: Anneleen
authors.initials: A
authors.lastname: Vuchelen
authors.forename: Elizabeth
authors.initials: E
authors.lastname: O'Day
authors.forename: Erwin
authors.initials: E
authors.lastname: De Genst
authors.forename: Els
authors.initials: E
authors.lastname: Pardon
authors.forename: Lode
authors.initials: L
authors.lastname: Wyns
authors.forename: Mireille
authors.initials: M
authors.lastname: Dumoulin
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
authors.forename: John
authors.initials: J
authors.lastname: Christodoulou
authors.forename: Shang-Te Danny
authors.initials: ST
authors.lastname: Hsu
mesh_terms: D000818:Animals; D002161:Camelids, New World; D002162:Camelus; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000276:immunology*; D051844:alpha-Synuclein / Q000276:immunology*
chemical_list: D057127:Single-Chain Antibodies; D051844:alpha-Synuclein