Molecular characterization of Tb, a new approach for an ancient Brucellaphage.
Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 +/- 2 nm diameter) and short tails (32 +/- 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage's basic mechanisms and practical applications.
Zhu
Cai-Zhong
CZ
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.|State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
Xiong
Hong-Yan
HY
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
Han
Jing
J
Institute of Combine Injury, School of Preventive Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
Cui
Bu-Yun
BY
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
Piao
Dong-Ri
DR
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
Li
Ya-Fei
YF
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
Jiang
Hai
H
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
Ren
Qian
Q
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
Ma
Xiang-Yu
XY
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
Chai
Ya-Ming
YM
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
Huang
Xia
X
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China.
Zhao
Hong-Yan
HY
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
Li
Lan-Yu
LY
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China.
D001483:Base Sequence; D002003:Brucella abortus / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry*; D008969:Molecular Sequence Data; D017902:Podoviridae / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry
D004279:DNA, Viral; D014764:Viral Proteins