Acid hydrolysis of sugarcane bagasse for lactic acid production.
In order to use sugarcane bagasse as a substrate for lactic acid production, optimum conditions for acid hydrolysis of the bagasse were investigated. After lignin extraction, the conditions were varied in terms of hydrochloric (HCl) or sulfuric (H(2)SO(4)) concentration (0.5-5%, v/v), reaction time (1-5h) and incubation temperature (90-120 degrees C). The maximum catalytic efficiency (E) was 10.85 under the conditions of 0.5% of HCl at 100 degrees C for 5h, which the main components (in gl(-1)) in the hydrolysate were glucose, 1.50; xylose, 22.59; arabinose, 1.29; acetic acid, 0.15 and furfural, 1.19. To increase yield of lactic acid production from the hydrolysate by Lactococcus lactis IO-1, the hydrolysate was detoxified through amberlite and supplemented with 7 g l(-1) of xylose and 7 g l(-1) of yeast extract. The main products (in gl(-1)) of the fermentation were lactic acid, 10.85; acetic acid, 7.87; formic acid, 6.04 and ethanol, 5.24.
Laopaiboon
Pattana
P
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand. patlao@kku.ac.th
Thani
Arthit
A
Leelavatcharamas
Vichean
V
Laopaiboon
Lakkana
L
D000143:Acids / Q000737:chemistry; D064753:Ammonium Hydroxide; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002482:Cellulose / Q000737:chemistry*; D005285:Fermentation; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D006878:Hydroxides / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D013294:Lactococcus lactis / Q000378:metabolism; D008031:Lignin / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000143:Acids; D006878:Hydroxides; D013464:Sulfuric Acids; D019344:Lactic Acid; D064753:Ammonium Hydroxide; D002482:Cellulose; D008031:Lignin; C027433:bagasse; C033158:sulfuric acid; D006851:Hydrochloric Acid