title: Promotion of triplex formation by 3'-amino-2'-O,4'-C-methylene bridged nucleic acid modification.
abstract: We examined the effect of 3'-amino-2'-O,4'-C-methylene bridged nucleic acid (3'-amino-2',4'-BNA) backbone modification of triplex-forming oligonucleotide (TFO) on the pyrimidine motif triplex formation at neutral pH, a condition where pyrimidine motif triplexes are unstable. The melting temperature of the pyrimidine motif triplex at pH 6.8 with 3'-amino-2',4'-BNA modified TFO was significantly higher than that observed with unmodified TFO. The 3'-amino-2',4'-BNA modification of TFO increased the thermal stability of the pyrimidine motif triplex at neutral pH. The present results certainly support the idea that the 3'-amino-2',4'-BNA modification of TFO could be a key chemical modification and may eventually lead to progress in therapeutic applications of the antigene strategy in vivo.
authors.affiliation: Department of Applied Chemistry, Faculty of Sciecne, Tokyo University of Science, Tokyo 162-8601, Japan.
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Sasaki
authors.forename: S M Abdur
authors.initials: SM
authors.lastname: Rahman
authors.forename: Norihiro
authors.initials: N
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imanishi
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Torigoe
mesh_terms: D001952:Bridged-Ring Compounds / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry; D011742:Pyrimidine Nucleotides / Q000737:chemistry*
chemical_list: D001952:Bridged-Ring Compounds; D009841:Oligonucleotides; D011742:Pyrimidine Nucleotides; C051802:triplex DNA; D004247:DNA