[Effect of Wenyang Huazhuo Tongluo recipe contained serum on proliferation and cell cycle of systemic sclerosis skin fibroblasts].
OBJECTIVE
To investigate the effect of "Wenyang Huazhuo Tongluo recipe" (WYHZTLR) on proliferation and cell cycle of systemic sclerosis (SSc) skin fibroblasts in vitro by serum pharmacologic technique.


METHODS
Prednisone, D-penicillamine and WYHZTLR contained serum from patients with SSc were respectively added into cultured normal dermal fibroblast cell-line and SSc lesional dermal fibroblast cell-line. Then the cells were incubated for 48 hours. Cell proliferation and cell cycle were examined by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry, respectively.


RESULTS
Western medicine Chinese medicine and integrated Chinese and western medicine could markedly inhibit the proliferation of normal dermal fibroblasts and SSc lesional dermal fibroblasts in vitro compared with control group (P < 0.05 or P < 0.01). But 20% serum in integrated Chinese and western medicine group could markedly inhibit the proliferation of fibroblasts compared with western medicine group (P < 0.01). Different drug-contained serum of WYHZTLR could markedly increase the percentage of G0-G1 stage cells (P < 0.01) and depress the percentage of sphase cells and G2-M stage cells compared with control group (P < 0.05 or P < 0.01).


CONCLUSIONS
WYHZTLR can block the progression of SSc skin fibroblasts into sphase and G2-M stage and inhibit the proliferation of SSc skin fibroblasts, and WYHZTLR was unselectively interfered with normal and SSc fibroblasts in vitro.
Bian
Hua
H
ZhangZhongjing College of TCM of Nanyang Institute of Technology, Nanyang 473004, China. biancrown@163.com
Fan
Yong-Sheng
YS
Lou
Lan-Hua
LH
Mao
Bing-Yu
BY
Shi
Jing-Yang
JY
D000328:Adult; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010396:Penicillamine / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011241:Prednisone / Q000494:pharmacology; D012595:Scleroderma, Systemic / Q000473:pathology*; D055815:Young Adult
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010396:Penicillamine; D011241:Prednisone