EDG3 and SHC3 on chromosome 9q22 are co-amplified in human ependymomas.
By qPCR we found that EDG3 and SHC3 were amplified in 60% of ependymomas but none in choroid plexus papillomas. In ependymomas EDG3 and SHC3 amplification increased Shc3 protein levels while EDG3 was less affected. Both proteins were co-immunoprecipitated from ependymoma and Shc3 was tyrosine phosphorylated thus presumably active. We showed by digestion with N-glycosidase-F that EDG3 was glycosylated indicating that EDG3 protein was not retained in the endoplasmic reticulum. The co-immunoprecipitation of Shc3 and EDG3 proteins from ependymomas with amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo.
Magrassi
Lorenzo
L
Neurochirurgia Dipartimento di Scienze Chirurgiche Università di Pavia Fondazione IRCCS Policlinico S. Matteo, Pavia, Italy. magrassi@igm.cnr.it
Marziliano
Nicola
N
Inzani
Frediano
F
Cassini
Pamela
P
Chiaranda
Ilaria
I
Skrap
Miran
M
Pizzolito
Stefano
S
Arienta
Cesare
C
Arbustini
Eloisa
E
D015153:Blotting, Western; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D004806:Ependymoma / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D049349:Receptors, Lysosphingolipid / Q000235:genetics*; D055765:Shc Signaling Adaptor Proteins / Q000235:genetics*; D000071427:Src Homology 2 Domain-Containing, Transforming Protein 3
D049349:Receptors, Lysosphingolipid; C122029:SHC3 protein, human; D055765:Shc Signaling Adaptor Proteins; D000071427:Src Homology 2 Domain-Containing, Transforming Protein 3