Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis.
Mariner eukaryotic elements transpose randomly and independently of any host factors, making them ideal tools for random mutagenesis in bacteria, including genetically intractable microorganisms. The transposable element Himar1, a member of the mariner family of transposons, originally isolated from the horn fly (Haematobia irritans), has thus been extensively used to generate large numbers of insertion mutants. Transposon-based approaches greatly facilitate studies of bacterial biology. We summarize the current mariner-based transposon tools and techniques for conducting genetic studies in bacteria.
Picardeau
Mathieu
M
Unité de Biologie des Spirochètes, Institut Pasteur, 28 rue du docteur Roux, 75724 Paris Cedex 15, France. mathieu.picardeau@pasteur.fr
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005821:Genetic Techniques; D005822:Genetic Vectors; D008958:Models, Molecular; D008968:Molecular Conformation; D009117:Muscidae / Q000235:genetics*; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D019895:Transposases / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases