Improved antioxidant and anti-inflammatory potential in mice consuming sour cherry juice (Prunus Cerasus cv. Maraska).
The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties.
Sarić
Ana
A
Division of Molecular Medicine, Rudjer Bosković Institute, Bijenicka 54, Zagreb 10000, Croatia.
Sobocanec
Sandra
S
Balog
Tihomir
T
Kusić
Borka
B
Sverko
Visnja
V
Dragović-Uzelac
Verica
V
Levaj
Branka
B
Cosić
Zrinka
Z
Macak Safranko
Zeljka
Z
Marotti
Tatjana
T
D000818:Animals; D000872:Anthocyanins / Q000032:analysis / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000097:blood; D005638:Fruit; D005979:Glutathione Peroxidase / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008264:Macrophages; D051379:Mice; D008808:Mice, Inbred CBA; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism*
D000872:Anthocyanins; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D002374:Catalase; D005979:Glutathione Peroxidase; D051546:Cyclooxygenase 2; D013482:Superoxide Dismutase