title: Ectomycorrhizal species associated with Pinus radiata in New Zealand including novel associations determined by molecular analysis.
abstract: Ectomycorrhizal (ECM) associates of the exotic plantation species Pinus radiata were investigated above and below ground over two years in the North Island of New Zealand. ECM species were identified using morphological and molecular (restriction fragment length polymorphism and DNA sequencing) analysis. Eighteen ECM species were observed fruiting above ground; 19 ECM species were identified below ground. In the above ground study, Wilcoxina mikolae, Rhizopogon pseudoroseolus and Inocybe sindonia were noted for the first time as ECM associates of P. radiata in New Zealand. Below ground, the species W. mikolae, R. pseudoroseolus, Rhizopogon luteorubescens, Pseudotomentella sp., Pseudotomentella tristis and Tomentella sp. were found as new associates of P. radiata in New Zealand. Additionally, six ECM types were found that could not be identified with molecular analysis. The putative ECM taxa Tricholoma pessundatum, Laccaria laccata and Hebeloma crustuliniforme were examined by molecular analysis, and species identifications were proposed to be changed to Tricholoma sp., L. laccata and Hebeloma sp. for specimens associated with P. radiata in New Zealand. The species identity of I. sindonia, previously unidentified to species level, was determined with direct sequencing.
authors.affiliation: Bio-Protection Research Centre, Lincoln University, Lincoln, New Zealand. Katrin.Walbert@scionresearch.com
authors.forename: Katrin
authors.initials: K
authors.lastname: Walbert
authors.forename: Tod D
authors.initials: TD
authors.lastname: Ramsfield
authors.forename: Hayley J
authors.initials: HJ
authors.lastname: Ridgway
authors.forename: E Eirian
authors.initials: EE
authors.lastname: Jones
mesh_terms: D044822:Biodiversity; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D038821:Mycorrhizae; D009520:New Zealand; D028223:Pinus / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer