Aqueous-phase quantitative NMR determination of amino acid enantiomer ratio by 13C-NMR using chiral neodymium shift reagent.
A neodymium-(S)-PDTA (PDTA = N,N,N',N'-tetrakis[(hydroxycarbonyl)methyl]-1,2-diaminopropane) complex was found exceptionally useful in the quantitative determination of enantiomer ratios of water-soluble natural amino acids by (13)C-NMR. The method is demonstrated on mixtures of L- and D-enantiomers of various amino acids. The interactions of the chiral shift reagent with the amino acid molecules were rationalized by molecular orbital calculations.
Florini
Nicola
N
Department of Chemistry, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy.
Faglioni
Francesco
F
Zucchi
Claudia
C
Caglioti
Luciano
L
Pályi
Gyula
G
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D009354:Neodymium / Q000737:chemistry*; D013237:Stereoisomerism
D000596:Amino Acids; D007202:Indicators and Reagents; D009354:Neodymium