A possible new diagnostic biomarker in early diagnosis of Alzheimer's disease.
Early diagnosis in patients with Alzheimer's disease (AD) is of great importance since only a sufficient treatment in early stages of this disease helps to keep patients in an autonomous state for as long as possible. Until now, there is no single diagnostic biomarker for AD derived from material routinely obtained. In this study, proton nuclear magnetic resonance (1H-NMR) spectra of the cerebrospinal fluid (CSF) of AD patients were compared with 1H-NMR spectra of the CSF of healthy control subjects using a metabonomic approach. The 1H-NMR spectra of CSF of AD patients showed specific multiplets at 2.15 ppm and 2.45 ppm, which could not be detected in the majority (59.3% and 70.4%, respectively) of healthy control subjects (positive likelihood ratio (+LR) 2.33, 95% CI [1.46, 3.72], p<0.01; +LR 3.22, 95% CI [1.78, 5.78], p<0.01). Moreover, CSF 1H-NMR spectra of AD patients showed specific resonances at 7.03 ppm (+LR 3.38, 95% CI [1.60, 7.14], p<0.05), 7.19 ppm (+LR 2.89, 95% CI [1.46, 5.74], p<0.05), 7.43 ppm (+LR 3.15, 95% CI [1.47, 6.75], p<0.05), and at 7.91 ppm (+LR 3.38, 95% CI [1.60, 7.14], p<0.01). CSF 1H-NMR spectroscopy is obviously a capable method for detection and quantification of substances in the CSF of AD patients even without the knowledge of molecular structures. These substances can be used as a biomarker in the early diagnosis of AD. We assume that this biomarker will simplify the diagnosis of AD, especially in early stages of the disease.
Kork
Felix
F
Department of Anesthesiology and Intensive Care Medicine, Charité-Universitaetsmedizin Berlin, Campus Virchow-Klinikum and Campus Charité Mitte, Berlin, Germany.
Holthues
Jan
J
Hellweg
Rainer
R
Jankowski
Vera
V
Tepel
Martin
M
Ohring
Renate
R
Heuser
Isabella
I
Bierbrauer
Jeffrey
J
Peters
Oliver
O
Schlattmann
Peter
P
Zidek
Walter
W
Jankowski
Joachim
J
D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000473:pathology; D015415:Biomarkers / Q000097:blood* / Q000134:cerebrospinal fluid*; D005260:Female; D006624:Hippocampus / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D009483:Neuropsychological Tests; D009929:Organ Size
D015415:Biomarkers