DHEA-Bodipy--a functional fluorescent DHEA analog for live cell imaging.
The androgen dehydroepiandrosterone (DHEA) has been reported to protect neuronal cells against dysfunction and apoptosis. Several signaling pathways involved in these effects have been described but little is known about the intracellular trafficking of DHEA. We describe design, synthesis and characterization of DHEA-Bodipy, a novel fluorescent DHEA analog. DHEA-Bodipy proved to be a functional DHEA derivative: DHEA-Bodipy (i) induced estrogen receptor alpha-mediated gene activation, (ii) protected PC12 rat pheochromocytoma cells against serum-deprivation-induced apoptosis, and (iii) induced stress fibers and focal adhesion contacts in SH-SY5Y human neuroblastoma cells. DHEA-Bodipy bound rapidly and specifically to plasma membranes of living PC12 cells. We analyzed metabolism and trafficking of DHEA-Bodipy in human neuroblastoma cells. DHEA-Bodipy is the first functional fluorescent DHEA derivative suitable for live cell imaging of intracellular DHEA transport and localization.
Lemcke
Susanne
S
Institute of Biochemistry, University of Mainz, Becherweg 30, 55099 Mainz, Germany.
Hönnscheidt
Christoph
C
Waschatko
Gustav
G
Bopp
Anita
A
Lütjohann
Dieter
D
Bertram
Norbert
N
Gehrig-Burger
Katja
K
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001692:Biological Transport; D001896:Boron Compounds / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D015394:Molecular Structure; D009447:Neuroblastoma; D016716:PC12 Cells; D051381:Rats
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D047628:Estrogen Receptor alpha; D005456:Fluorescent Dyes; D003687:Dehydroepiandrosterone