title: Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases.
abstract: Prostate cancer remains the most common malignancy among men in United States, and there is no remedy currently available for the advanced stage hormone-refractory cancer. This is partly due to the incomplete understanding of androgen-regulated proteins and their encoded functions. Whole-cell proteomes of androgen-starved and androgen-treated LNCaP cells were analyzed by semi-quantitative MudPIT ESI- ion trap MS/MS and quantitative iTRAQ MALDI- TOF MS/MS platforms, with identification of more than 1300 high-confidence proteins. An enrichment-based pathway mapping of the androgen-regulated proteomic data sets revealed a significant dysregulation of aminoacyl tRNA synthetases, indicating an increase in protein biosynthesis- a hallmark during prostate cancer progression. This observation is supported by immunoblot and transcript data from LNCaP cells, and prostate cancer tissue. Thus, data derived from multiple proteomics platforms and transcript data coupled with informatics analysis provides a deeper insight into the functional consequences of androgen action in prostate cancer.
authors.affiliation: Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Adaikkalam
authors.initials: A
authors.lastname: Vellaichamy
authors.forename: Arun
authors.initials: A
authors.lastname: Sreekumar
authors.forename: John R
authors.initials: JR
authors.lastname: Strahler
authors.forename: Theckelnaycke
authors.initials: T
authors.lastname: Rajendiran
authors.forename: Jindan
authors.initials: J
authors.lastname: Yu
authors.forename: Sooryanarayana
authors.initials: S
authors.lastname: Varambally
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Gilbert S
authors.initials: GS
authors.lastname: Omenn
authors.forename: Arul M
authors.initials: AM
authors.lastname: Chinnaiyan
authors.forename: Alexey I
authors.initials: AI
authors.lastname: Nesvizhskii
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry*; D000728:Androgens / Q000378:metabolism*; D045744:Cell Line, Tumor; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008297:Male; D008954:Models, Biological; D011467:Prostate / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000473:pathology*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000728:Androgens; D000604:Amino Acyl-tRNA Synthetases