Structure of a novel highly branched alpha-glucan enzymatically produced from maltodextrin.
The bacterial strain PP710, isolated from soil and identified as Paenibacillus species, produced a low-digestibility alpha-glucan containing a large amylase-resistant portion. This alpha-glucan was obtained in high yields from maltodextrin (dextrose equivalent 3) by using the condensed culture supernatant of the strain as the enzyme preparation. The water-soluble dietary fiber content of the low-digestibility alpha-glucan was 80.2%, and showed resistance to a rat intestinal enzyme preparation. The alpha-glucan was found to be a novel highly branched alpha-glucan by acid hydrolysis, NMR analysis, gel permeation chromatography, methylation analysis, and enzymatic digestion.
Tsusaki
Keiji
K
Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc., Naka-ku, Okayama 702-8006, Japan. tsusaki@hayashibara.co.jp
Watanabe
Hikaru
H
Nishimoto
Tomoyuki
T
Yamamoto
Takuo
T
Kubota
Michio
M
Chaen
Hiroto
H
Fukuda
Shigeharu
S
D000818:Animals; D003910:Dextranase / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D001614:beta-Amylase / Q000378:metabolism
D004798:Enzymes; D005936:Glucans; D005960:Glucosides; D011134:Polysaccharides; C008315:maltodextrin; D006026:Glycoside Hydrolases; D003910:Dextranase; D001614:beta-Amylase; C091051:glucan 1,6-alpha-isomaltosidase