[Study on fast quality control in extracting process of Paeonia lactiflora using near infrared spectroscopy].
OBJECTIVE
To set up a novel method for fast analysis of active components in water extracting process of Paeonia lactiflora with near infrared spectroscopy (NIRS).


METHODS
HPLC was used as the reference method to determine the content of Paeoniflorin and a multivariate calibration model based on PLS algorithm was developed to analyze the correlation between the spectra and the corresponding values determined by the reference method.


RESULTS
The correlation coefficient of the calibration model was 0.996 2, and the predicted coefficient was 0.989 5. The RMSEC and RMSEP were 0.109 g x L(-1) and 0.138 g x L(-1), respectively, and the RSEP was 5.6%.


CONCLUSIONS
The method mentioned above is proved to be convenient, rapid and nondestructive, accurate and reliable, and is applicable for fast analysis and monitoring of active components in extraction process of traditional Chinese medicine.
Chen
Xueying
X
Institute of Modern Chinese Medicine, Zhejiang University, Hangzhou 310058, China.
Li
Yerui
Y
Chen
Yong
Y
Wang
Longhu
L
Sun
Changhai
C
Liu
Xuesong
X
D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
D010936:Plant Extracts