title: Mapping the lipolytic proteome of adipose tissue using fluorescent suicide inhibitors.
abstract: Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl esters in animals, plants, and microorganisms. In this chapter we describe a tool for the concomitant analysis of lipases in complex proteomes. For this purpose, the target enzymes are selectively and covalently labelled with fluorescent suicide inhibitors. Stable lipid-protein complexes are formed that are separated by gel electrophoresis and identified by mass spectrometry.
authors.affiliation: Institute of Biochemistry, University of Graz, Graz, Austria.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Schicher
authors.forename: Manfred
authors.initials: M
authors.lastname: Kollroser
authors.forename: Albin
authors.initials: A
authors.lastname: Hermetter
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry* / Q000201:enzymology; D000478:Alkylation; D000818:Animals; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D007525:Isoelectric Focusing; D008049:Lipase / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D010084:Oxidation-Reduction; D020543:Proteome; D053719:Tandem Mass Spectrometry
chemical_list: D005456:Fluorescent Dyes; D008055:Lipids; D020543:Proteome; D008049:Lipase