title: Stimulatory effect of growth hormone-releasing hormone (GHRH(1-29)NH2) on the proliferation, VEGF and chromogranin A secretion by human neuroendocrine tumor cell line NCI-H727 in vitro.
abstract: Growth hormone-releasing hormone (GHRH) and its receptors have been implicated in a variety of cellular processes like cell survival, proliferation, apoptosis, angiogenesis and neoplastic transformation of various non-pituitary tissues. Here, we investigated for the first time the in vitro effect of GHRH(1-29)NH2 on the proliferation and the secretion of vascular endothelial growth factor (VEGF) and chromogranin A by the human bronchial neuroendocrine tumor cells NCI-H727. GHRH(1-29)NH2 at the concentrations of 10(-8)-10(-6)M increased the proliferation of these cells and this effect was associated with a statistically significant increase in VEGF and chromogranin A secretion into the supernatants of the tested cells. Our findings indicate that GHRH functions as a trophic hormone for bronchial neuroendocrine (NET) tumors.
authors.affiliation: Department of General and Endocrinological Surgery, Copernicus Memorial Hospital, Pabianicka Street 62, 93-513 Lodz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Stepień
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sacewicz
authors.forename: Hanna
authors.initials: H
authors.lastname: Lawnicka
authors.forename: Roman
authors.initials: R
authors.lastname: Krupiński
authors.forename: Jan
authors.initials: J
authors.lastname: Komorowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Siejka
authors.forename: Henryk
authors.initials: H
authors.lastname: Stepień
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D053379:Chromogranin A / Q000378:metabolism*; D006801:Humans; D018358:Neuroendocrine Tumors / Q000378:metabolism*; D017337:Sermorelin / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D053379:Chromogranin A; D042461:Vascular Endothelial Growth Factor A; D017337:Sermorelin