title: [Relationship between glucocorticoid receptors and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura].
abstract: OBJECTIVE
  To identify the relationship between the expression of alpha and beta-isoforms of glucocorticoid receptors (GR) in peripheral blood mononuclear cells (PBMC) and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura (ITP).
  
  
  METHODS
  Real-time PCR was used to detect the expression of GR alpha and GR beta mRNA in PBMC from 30 children with ITP (glucocorticoid-sensitive, n=18; glucocorticoid-resistant, n=12) and 10 healthy children (control group). Enzyme immunoassay was used to measure plasma levels of total glucocorticoids.
  
  
  RESULTS
  There were no significant differences in PBMC GR alpha mRNA levels among the glucocorticoid sensitive, the glucocorticoid-resistant and the control groups. Compared with the glucocorticoid-sensitive and the control groups, the expression of GR beta mRNA in the glucocorticoid-resistant group was significantly up-regulated (p<0.01). Plasma total glucocorticoids level in the glucocorticoid-resistant group was found to be much higher than that in the glucocorticoid-sensitive and the control groups (p<0.01).
  
  
  CONCLUSIONS
  The up-regulated expression of GR beta mRNA may associated with glucocorticoid resistance in children with ITP.
authors.affiliation: Department of Hematology, Shenzhen Children' s Hospital, Shenzhen, Guangdong 518020, China. ljlwyn@hotmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Hui-Rong
authors.initials: HR
authors.lastname: Mai
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Yuan
authors.forename: Hong-Song
authors.initials: HS
authors.lastname: Shi
authors.forename: Si-Xi
authors.initials: SX
authors.lastname: Liu
authors.forename: Chang-Gang
authors.initials: CG
authors.lastname: Li
mesh_terms: D002648:Child; D002675:Child, Preschool; D004351:Drug Resistance; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008297:Male; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000188:drug therapy*; D012333:RNA, Messenger / Q000032:analysis; D011965:Receptors, Glucocorticoid / Q000097:blood* / Q000235:genetics
chemical_list: D005938:Glucocorticoids; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid