Association study on two vitamin D receptor gene polymorphisms and vitamin D metabolites in multiple sclerosis.
BACKGROUND
An association of two single-nucleotide polymorphisms (SNPs) of the vitamin D receptor (VDR) gene, Apal (rs7975232) and Taql (rs731236), with multiple sclerosis (MS) has been reported in a Caucasian population. Another SNP of the VDR gene, Fokl (rs10735810), has been associated with serum levels of 25-hydroxyvitamin D [25(OH)D].


OBJECTIVE
Since 25(OH)D status has been associated with MS incidence, we assessed the association Apal and Taql with the serum levels of 25(OH)D and 1,25(OH)(2)D, and MS in our population.


METHODS
We determined the two SNPs as well as the summer and winter period serum levels of 25(OH)D and 1,25(OH)(2)D in 212 MS patients. Additionally, we genotyped 289 healthy controls and determined the metabolite levels.


RESULTS
The genotype and allele distribution of the two VDR gene SNPs did not differ between patients and controls. There was no association of the Apal and Taql SNPs with 25(OH)D levels, 1,25(OH)(2)D levels, or 1,25(OH)(2)D/25(OH)D ratios in patients or controls.


CONCLUSIONS
We found no association of the Apal and Taql VDR gene SNPs with MS or with vitamin D metabolism in our population. Further research should assess the complex interaction between vitamin D, the VDR, and susceptibility to MS.
Smolders
Joost
J
School for Mental Health and Neuroscience, Maastricht University Medical Center, Maastricht, The Netherlands. j.smolders@mumc.nl
Damoiseaux
Jan
J
Menheere
Paul
P
Tervaert
Jan Willem Cohen
JW
Hupperts
Raymond
R
D000328:Adult; D002117:Calcitriol / Q000097:blood; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics*; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism
D018167:Receptors, Calcitriol; D014807:Vitamin D; C104450:25-hydroxyvitamin D; D002117:Calcitriol