Uber infektiöse Substrukturen aus Bakteriophagen. VI. A simple method for separating the replicative form from single-stranded phiX174 DNA.
A selective adsorption method is presented which allows a rapid separation of phiX174 replicative form DNA from single-stranded phiX174 phage DNA, facilitating the handling of a large number of samples. The single-stranded phiX174 DNA is selectively adsorbed to silicic acid coated with methylated albumin. After removal of the adsorbent by sedimentation, at least 40% of the input replicative form DNA is recovered in the supernatant fraction; the ratio of replicative form to single-stranded phage DNA is increased 20- to 200-fold. The potential of the method for separating other nucleic acids is discussed.
Benzinger
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Max-Planck-Institut für Biochemie Goethestrasse 31, Munich, Germany.
Jaenisch
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R
Hofschneider
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PH
D000418:Albumins / Q000737:chemistry; D010584:Bacteriophage phi X 174 / Q000737:chemistry* / Q000235:genetics; D002845:Chromatography / Q000379:methods; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry / Q000821:virology*; D008745:Methylation
D000418:Albumins; D004277:DNA, Single-Stranded; D004279:DNA, Viral