Spectroscopic investigation of sulfonate phthalocyanine to probe enzyme reactions for heavy metals detection.
Optical absorption and Raman spectra of the sulfonated copper phthalocyanine (CuTsPc) layer were exploited for detection of cadmium (Cd) contaminants in water. Acetylcholine esterase was immobilized by freely suspending them in calcium alginate microbeads and this gel was then spincoated on the drop cast sulfonated copper phthalocyanine film on a glass substrate to form a bilayer. The inhibition of catalytic reaction between acetylcholine chloride and enzyme due to Cd contaminants was monitored by recording changes in spectra of drop cast CuTsPc as an indicator. The detection limit of cadmium content in water was found to be 1 ppm.
Chaure
Shweta
S
School of Engineering & Material Science, Queen Mary, University of London, London E1 4NS, UK.
Paul
Deepen
D
Vadagma
Pankaj
P
Ray
Asim K
AK
D000109:Acetylcholine / Q000032:analysis; D000110:Acetylcholinesterase / Q000737:chemistry; D000464:Alginates / Q000737:chemistry; D000465:Algorithms; D003300:Copper / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007202:Indicators and Reagents; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D019216:Metals, Heavy / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D014874:Water Pollutants, Chemical / Q000032:analysis
D000464:Alginates; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D007211:Indoles; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; C028418:tetrasulfophthalocyanine; D003300:Copper; D000110:Acetylcholinesterase; D000109:Acetylcholine