Analyzing and monitoring of phage-bacteria interaction using CE.
The utilization of CE for monitoring bacteria-phage interaction was investigated in this study. Streptococcus thermophilus and Lactobacillus bulgaricus strains and their phages were used as model bacteria and phages for the purpose of validation in this study. CE with heterogeneous polymer polyethylene oxide was utilized for the separation of intact bacteria and investigation of phage-bacteria interaction. An intact phage detection was carried out with CZE by adding SDS in the running buffer. Calibration graphs of bacteria and phages were obtained with R(2) values of 0.963 and 0.937, respectively. S. thermophilus strain was infected with its virulent phage B3-X18 for investigation of phage-bacteria interaction. It was observed in capillary electropherogram that the culture was lysed depending on the multiplicity of infection value and it showed to be completely lysed when the multiplicity of infection value was 10. The interaction of S. thermophilus strain with L. bulgaricus phage was also investigated by using a CE and a microbiological method and it was observed that the L. bulgaricus phage attached itself to the cell wall of S. thermophilus strain without damaging the cell.
Soykut
Esra Acar
EA
Department of Food Engineering, Hacettepe University, Beytepe, Ankara, Turkey.
Boyaci
Ismail Hakki
IH
D001435:Bacteriophages / Q000302:isolation & purification / Q000502:physiology*; D002473:Cell Wall / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D006790:Host-Parasite Interactions / Q000502:physiology*; D048668:Lactobacillus delbrueckii / Q000302:isolation & purification / Q000502:physiology*; D048249:Streptococcus thermophilus / Q000302:isolation & purification / Q000502:physiology*