title: MALDI-TOF MS-based drug susceptibility testing of pathogens: the example of Candida albicans and fluconazole.
abstract: MALDI-TOF MS can be used for the identification of microorganism species. We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The method was accurate, and reliable, and showed full agreement with the Clinical Laboratory Standards Institute's reference method. This proof-of-concept demonstration highlights the potential for this approach to test other pathogens.
authors.affiliation: INSERM, UMRS 945 Paris, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Marinach
authors.forename: Alexandre
authors.initials: A
authors.lastname: Alanio
authors.forename: Martine
authors.initials: M
authors.lastname: Palous
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Kwasek
authors.forename: Arnaud
authors.initials: A
authors.lastname: Fekkar
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Brossas
authors.forename: Sophie
authors.initials: S
authors.lastname: Brun
authors.forename: Georges
authors.initials: G
authors.lastname: Snounou
authors.forename: Christophe
authors.initials: C
authors.lastname: Hennequin
authors.forename: Dominique
authors.initials: D
authors.lastname: Sanglard
authors.forename: Annick
authors.initials: A
authors.lastname: Datry
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Golmard
authors.forename: Dominique
authors.initials: D
authors.lastname: Mazier
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D015725:Fluconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole