Influenza recombinant vaccine: matrix protein M1 on the platform of the adenovirus dodecahedron.
We propose a novel influenza vaccine composed of the adenovirus dodecahedron (Dd) as delivery platform carrying an internal influenza matrix protein M1. To attach the antigen to the vector we used WW domains interacting with Dd. Successful internalization of the Dd-M1WW complex was observed using biochemical and cell biology techniques. We show here that the complex of Dd with antigen is a potent activator of human myeloid dendritic cells (MDC), and that it is efficiently presented by MDC to M1-specific CD8+ T lymphocytes. These results show that proposed vaccine model is feasible and that adenovirus dodecahedron is a potent delivery platform for foreign antigens to human cells.
Naskalska
A
A
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawinskiego 5a, 02-106 Warsaw, Poland.
Szolajska
E
E
Chaperot
L
L
Angel
J
J
Plumas
J
J
Chroboczek
J
J
D000256:Adenoviridae / Q000276:immunology*; D000595:Amino Acid Sequence; D017951:Antigen Presentation / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000096:biosynthesis / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology*
D007252:Influenza Vaccines; C488936:M1 protein, Influenza A virus; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014763:Viral Matrix Proteins