title: Real-time fluorescence detection of exoribonucleases.
abstract: The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases.
authors.affiliation: Institut de Biologie Physico-Chimique, CNRS UPR 9073, Université de Paris 7-Denis Diderot, 75005 Paris, France.
authors.forename: Flore
authors.initials: F
authors.lastname: Sinturel
authors.forename: Olivier
authors.initials: O
authors.lastname: Pellegrini
authors.forename: Song
authors.initials: S
authors.lastname: Xiang
authors.forename: Liang
authors.initials: L
authors.lastname: Tong
authors.forename: Ciarán
authors.initials: C
authors.lastname: Condon
authors.forename: Lionel
authors.initials: L
authors.lastname: Bénard
mesh_terms: D001483:Base Sequence; D005095:Exoribonucleases / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D005095:Exoribonucleases