title: Fluorescent properties of acridonyl group in DNA duplex.
abstract: We designed and synthesized a nucleoside derivative in which the nucleobase is replaced with acridone. The nucleoside derivative was incorporated into an oligodeoxyribonucleotide (ODN), and its influence on the stability of ODN hybrids and its fluorescent properties in a DNA duplex were measured by thermodynamic analysis and fluorescent spectroscopy. These results showed that the acridonyl group could distinguish the type of nucleobase paired from fluorescent intensity, although the hybrid stability did not depend significantly on the types of nucleobases paired.
authors.affiliation: Graduate School of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan. kuwahara@chem-bio.gunma-u.ac.jp
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tajima
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kitazume
authors.forename: Keisuke
authors.initials: K
authors.lastname: Anahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
authors.forename: Seiji
authors.initials: S
authors.lastname: Tobita
mesh_terms: D054831:Acridones / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
chemical_list: D054831:Acridones; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D004247:DNA