Applications of isothermal titration calorimetry in biophysical studies of G-quadruplexes.
G-quadruplexes are higher-order nucleic acids structures formed by G-rich sequences that are stabilized by tetrads of hydrogen-bonded guanine bases. Recently, there has been growing interest in the study of G-quadruplexes because of their possible involvement in many biological processes. Isothermal titration calorimetry (ITC) has been proven to be a useful tool to study the energetic aspects of G-quadruplex interactions. Particularly, ITC has been applied many times to determine the thermodynamic properties of drug-quadruplex interactions to screening among various drugs and to address drug design. In the present review, we will focus on the ITC studies of G-quadruplex structures and their interaction with proteins and drugs and the most significant results will be discussed.
Pagano
Bruno
B
Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via Ponte Don Melillo, 84084, Fisciano (SA), Italy.
Mattia
Carlo Andrea
CA
Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via Ponte Don Melillo, 84084, Fisciano (SA), Italy.
Giancola
Concetta
C
Dipartimento di Chimica, Università di Napoli Federico II, via Cintia, 80126, Napoli, Italy.
D002151:Calorimetry / Q000379:methods*; D054856:G-Quadruplexes; D013816:Thermodynamics