EIN2, the central regulator of ethylene signalling, is localized at the ER membrane where it interacts with the ethylene receptor ETR1.
Genetic studies have identified the membrane protein EIN2 (ethylene insensitive 2) as a central component of ethylene signalling in Arabidopsis. In addition, EIN2 might take part in multiple hormone signalling pathways and in response to pathogens as demonstrated by recent genetic and biochemical studies. Here we show, by an integrated approach using in vivo and in vitro fluorescence techniques, that EIN2 is localized at the ER (endoplasmic reticulum) membrane where it shows specific interaction with the ethylene receptor protein ETR1.
Bisson
Melanie M A
MM
Department of Plant Biochemistry, Heinrich-Heine University, 40225 Düsseldorf, Universitätsstrasse 1, Germany.
Bleckmann
Andrea
A
Allekotte
Silke
S
Groth
Georg
G
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002942:Circular Dichroism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D018613:Microscopy, Confocal; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D013050:Spectrometry, Fluorescence; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; C119963:EIN2 protein, Arabidopsis; C494590:ETR1 protein, Arabidopsis; D005030:Ethylenes; D010937:Plant Growth Regulators; D011956:Receptors, Cell Surface; C036216:ethylene