The synaptic remodeling between regenerated perforant pathway and granule cells in slice culture.
In order to understand the synaptic remodeling in the course of axonal regeneration, the synaptic remodeling of the perforant path in hippocampus was investigated in the present study with entorhino-hippocampal coculture, DiI DiOlistic assay and transmission electron microscopy. The results showed that the regeneration of the perforant pathway occurred in entorhino-hippocampal slice coculture, and putative synaptic contacts formed between the regenerated fibers and dendritic spines of granule cells. Ultrastructural analysis confirmed the formation of new synaptic contacts. In conclusion, the synaptic formation implicated in the neuroregeneration could integrate into the network in CNS.
Yu
Dong-Ming
DM
Institute of Neurobiology and Laboratory of Neurobiology, Henan University, 475004, Kaifeng, Henan Province, People's Republic of China.
Tang
Wen-Chun
WC
Wu
Ping
P
Deng
Tong-Xing
TX
Liu
Bin
B
Li
Ming-Shan
MS
Deng
Jin-Bo
JB
D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D018728:Entorhinal Cortex / Q000166:cytology* / Q000502:physiology; D006624:Hippocampus / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009416:Nerve Regeneration / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D019580:Perforant Pathway / Q000166:cytology / Q000502:physiology; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure