AAV retinal transduction in a large animal model species: comparison of a self-complementary AAV2/5 with a single-stranded AAV2/5 vector.
OBJECTIVE
To compare self-complementary (sc) and single-stranded (ss) adeno-associated viral 2/5 (AAV2/5) vectors for retinal cell transduction in the dog when delivered by subretinal injection.


METHODS
ScAAV2/5 and ssAAV2/5 vectors encoding enhanced green fluorescent protein (GFP) under control of the chicken beta actin promoter were prepared to the same titer. Equal amounts of viral particles were delivered into the subretinal spaces of both eyes of two dogs. In each dog, one eye received the scAAV2/5 and the other the ssAAV2/5. In vivo expression of GFP was monitored ophthalmoscopically. The dogs were sacrificed, and their retinas were examined by fluorescent microscopy and immunohistochemistry to determine GFP expression patterns and to assay for glial reactivity.


RESULTS
GFP expression in the scAAV2/5 injected eyes was detectable at a much earlier time point than in the ssAAV2/5 injected eyes. Expression of GFP was also at higher levels in the scAAV2/5-injected eyes. Expression levels remained stable for the seven month duration of the study. The types of cells transduced by both vectors were similar; there was strong reporter gene expression in the RPE and photoreceptors, although not all cones in the transduced area expressed GFP. Some horizontal and Müller cells were also transduced.


CONCLUSIONS
When delivered by subretinal injection in the dog, scAAV2/5 induces faster and stronger transgene expression than ssAAV2/5. The spectrum of retinal neurons transduced is similar between the two vectors. These results confirm in a large animal model those previously reported in the mouse. ScAAV2/5 shows promise for use in the treatment of conditions where a rapid transgene expression is desirable. Furthermore, it may be possible to use a lower number of viral particles to achieve the same effect compared with ssAAV2/5 vectors.
Petersen-Jones
S M
SM
Department of Small Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA. Peter315@cvm.msu.edu
Bartoe
J T
JT
Fischer
A J
AJ
Scott
M
M
Boye
S L
SL
Chiodo
V
V
Hauswirth
W W
WW
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D000229:Dependovirus / Q000235:genetics*; D004285:Dogs; D005260:Female; D005822:Genetic Vectors / Q000235:genetics*; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D009457:Neuroglia / Q000166:cytology; D012160:Retina / Q000166:cytology; D014161:Transduction, Genetic
D018076:DNA, Complementary; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins