title: Cytogenetic studies of three European species of Centaurea L. (Asteraceae).
abstract: Cytogenetic analysis of several populations of Centaurea jacea (2n = 4x = 44), C. oxylepis (2n = 4x = 44) and C. phrygia (2n = 2x = 22) was performed using flow cytometry, differential chromosome staining and FISH. In all species Arabidopsis-type telomeric repeats hybridized only to the terminal part of chromosomes. In C. phrygia three pairs and in C. oxylepis six pairs of chromosomes revealed the hybridization signals of 45S rDNA. Centaurea jacea showed polymorphism in the 45S rDNA loci number, five or six pairs of sites were observed. 5S rDNA loci were located in two pairs of chromosomes in C. phrygia. In C. jacea and C. oxylepis the number and position of 5S rDNA loci were the same: three pairs located interstitially and one terminally. The genome size of the diploid C. phrygia was established as 2.14 pg/2C. The genomes of tetraploid species were nearly two times larger and genome size polymorphism was observed among C. jacea populations.
authors.affiliation: Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Dydak
authors.forename: Bozena
authors.initials: B
authors.lastname: Kolano
authors.forename: Teresa
authors.initials: T
authors.lastname: Nowak
authors.forename: Dorota
authors.initials: D
authors.lastname: Siwinska
authors.forename: Jolanta
authors.initials: J
authors.lastname: Maluszynska
mesh_terms: D031179:Centaurea / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics*; D020732:Cytogenetic Analysis; D018744:DNA, Plant / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal