MALDI-TOF-MS analysis of small molecules using modified mesoporous material SBA-15 as assisted matrix.
Mesoporous silica, SBA-15 was successfully functionalized with quinoline moiety, and was applied as a matrix in the MALDI-TOF-MS analysis of small molecules. The modified SBA-15 material [SBA-15-8-(3-(triethoxysilyl)propoxy) quinoline, SBA-15-8QSi] was obtained by using calcined SBA-15 and 8-hydroxy quinoline. The structure of the functionalized mesoporous material was systemically characterized by TEM, the N(2) adsorption-desorption isotherm technique and FT-IR spectra. Compared with DHB and SBA-15, SBA-15-8QSi demonstrated several advantages in the analysis of small molecules with MALDI-TOF-MS, such as less background interference ions, high homogeneity, and better reproducibility. Based on these results, the various analytical parameters were optimized. The ideal operating conditions were (1) methanol used as the dissolving solvent; (2) sample first dropping method; (3) a ratio between the analyte and the matrix of 3.5:10. Under these optimization conditions, a low detection limit (8 pmol for L-Arginine-HCl) and high reproducibility (< or = 29%) were obtained. This technique was successfully applied to the analysis of various types of small molecules, such as saccharides, amino acids, metabolites, and natural honey.
Li
Xiuhua
X
Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Jilin, China.
Wu
Xue
X
Kim
Ji Man
JM
Kim
Sung Soo
SS
Jin
Mingshi
M
Li
Donghao
D
D000596:Amino Acids / Q000032:analysis*; D019411:Clinical Laboratory Techniques; D005841:Gentisates / Q000737:chemistry; D006722:Honey / Q000032:analysis*; D000432:Methanol / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011804:Quinolines / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000032:analysis*; D013499:Surface Properties
D000596:Amino Acids; D005841:Gentisates; D011804:Quinolines; C509969:SBA-15; D012997:Solvents; D013395:Sucrose; D012822:Silicon Dioxide; C010925:2,5-dihydroxybenzoic acid; D000432:Methanol