A new assay format for NF-kappaB based on a DNA triple helix and a fluorescence resonance energy transfer.
Herein we report a feasibility study for a new concept to detect DNA binding protein NF-kappaB based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.
Altevogt
Dominik
D
Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, Freiburg, 79104, Germany.
Hrenn
Andrea
A
Kern
Claudia
C
Clima
Lilia
L
Bannwarth
Willi
W
Merfort
Irmgard
I
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005240:Feasibility Studies; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D019169:Jurkat Cells; D016328:NF-kappa B / Q000032:analysis* / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D044366:Transition Temperature
D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; C051802:triplex DNA; D004247:DNA