[The introduction of the 9alpha-hydroxy group into androst-4-en-3,17-dione using a new actinobacterium strain].
A 9alpha-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4-20 g/l) into 9alpha-hydroxy-AD over 20-48 h. This culture was able to grow and perform AD 9alphahydroxylation at a concentration of dimethyl formamide up to 9%. Crystalline 9alpha-hydroxy-AD was isolated with a yield of over 90%.
Rodina
N V
NV
Andriushina
V A
VA
Stytsenko
T S
TS
Turova
T P
TP
Baslerov
R V
RV
Panteleeva
A N
AN
Voĭshvillo
N E
NE
D000735:Androstenedione / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004126:Dimethylformamide / Q000494:pharmacology; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D006900:Hydroxylation / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D000735:Androstenedione; D004126:Dimethylformamide; D006867:Hydrolases