Single-molecule magnetic tweezers studies of type IB topoisomerases.
The past few years have seen the application of single-molecule force spectroscopy techniques to the study of topoisomerases. Magnetic tweezers are particularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.
Lipfert
Jan
J
Kavli Institute of NanoScience, Delft University of Technology, Delft, Delft, Netherlands.
Koster
Daniel A
DA
Vilfan
Igor D
ID
Hage
Susanne
S
Dekker
Nynke H
NH
D004247:DNA / Q000737:chemistry / Q000378:metabolism; D053904:DNA Breaks, Single-Stranded; D004264:DNA Topoisomerases, Type I / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008280:Magnetics / Q000295:instrumentation / Q000379:methods; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D009690:Nucleic Acid Conformation; D012984:Software
D004247:DNA; D004264:DNA Topoisomerases, Type I