Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity.
In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors.
Nekrasov
Vladimir
V
The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
Li
Jing
J
Batoux
Martine
M
Roux
Milena
M
Chu
Zhao-Hui
ZH
Lacombe
Severine
S
Rougon
Alejandra
A
Bittel
Pascal
P
Kiss-Papp
Marta
M
Chinchilla
Delphine
D
van Esse
H Peter
HP
Jorda
Lucia
L
Schwessinger
Benjamin
B
Nicaise
Valerie
V
Thomma
Bart P H J
BP
Molina
Antonio
A
Jones
Jonathan D G
JD
Zipfel
Cyril
C
D017360:Arabidopsis / Q000276:immunology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism; D007113:Immunity, Innate; D010935:Plant Diseases / Q000276:immunology*; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C545106:EFR protein, Arabidopsis; D050956:HSP40 Heat-Shock Proteins; D051192:Receptors, Pattern Recognition; C545107:SDF2 protein, Arabidopsis; C077221:BIP protein, Arabidopsis