Estimation of methods of protein delivery into mammalian cells--a comparative study by electroporation and bioporter assay.
To test the efficiency of some alternative methods for protein delivery into live cells were used rEGFP (recombinant Enhanced Green Fluorescent Protein) and HeLa cells as model system. The compared methods, electroporation (up to 10%) and Bioporter (up to 20%), deliver proteins and peptides to the cells with efficiency, enough for functional studies of mammalian cells. Thus, the delivery by either of both methods, followed by fluorescence activated cell sorting (FACS) of the cell population, can give a sufficient number of uniformly loaded cells for different studies including transcription assays and gene therapy.
Todorova
R
R
Institute of Biophysics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria. todorova@obzor.bio21.bas.bg
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D016503:Drug Delivery Systems / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins / Q000494:pharmacology*; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins