Genotyping of 73 UM-SCC head and neck squamous cell carcinoma cell lines.
BACKGROUND
We established multiple University of Michigan Squamous Cell Carcinoma (UM-SCC) cell lines. With time, these have been distributed to other labs all over the world. Recent scientific discussions have noted the need to confirm the origin and identity of cell lines in grant proposals and journal articles. We genotyped the UM-SCC cell lines in our collection to confirm their unique identity.


METHODS
Early-passage UM-SCC cell lines were genotyped and photographed.


RESULTS
Thus far, 73 unique head and neck UM-SCC cell lines (from 65 donors, including 21 lines from 17 females) were genotyped. In 7 cases, separate cell lines were established from the same donor.


CONCLUSIONS
These results will be posted on the UM Head and Neck SPORE Tissue Core website for other investigators to confirm that the UM-SCC cells used in their laboratories have the correct features. Publications using UM-SCC cell lines should confirm the genotype.
Brenner
J Chad
JC
Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Graham
Martin P
MP
Kumar
Bhavna
B
Saunders
Lindsay M
LM
Kupfer
Robbi
R
Lyons
Robert H
RH
Bradford
Carol R
CR
Carey
Thomas E
TE
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm