Exercise effects on activities of Na(+),K(+)-ATPase, acetylcholinesterase and adenine nucleotides hydrolysis in ovariectomized rats.
Hormone deficiency following ovariectomy causes activation of Na(+),K(+)-ATPase and acetylcholinesterase (AChE) that has been related to cognitive deficits in experimental animals. Considering that physical exercise presents neuroprotector effects, we decide to investigate whether exercise training would affect enzyme activation in hippocampus and cerebral cortex, as well as adenosine nucleotide hydrolysis in synaptosomes from cerebral cortex of ovariectomized rats. Female adult Wistar rats were assigned to one of the following groups: sham (submitted to surgery without removal of the ovaries), exercise, ovariectomized (Ovx) and Ovx plus exercise. Thirty days after surgery, animals were submitted to one month of exercise training, three times per week. After, rats were euthanized, blood serum was collected and hippocampus and cerebral cortex were dissected. Data demonstrated that exercise reversed the activation of Na(+),K(+)-ATPase and AChE activities both in hippocampus and cerebral cortex of ovariectomized rats. Ovariectomy decreased AMP hydrolysis in cerebral cortex and did not alter adenine nucleotides hydrolysis in blood serum. Exercise per se decreased ADP and AMP hydrolysis in cerebral cortex. On the other hand, AMP hydrolysis in blood serum was increased by exercise in ovariectomized adult rats. Present data support that physical exercise might have beneficial effects and constitute a therapeutic alternative to hormone replacement therapy for estrogen deprivation.
Ben
Juliana
J
Laboratório de Neuroproteção e Doença Metabólica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, Porto Alegre, RS, Brazil.
Soares
Flávia Mahatma Schneider
FM
Cechetti
Fernanda
F
Vuaden
Fernanda Cenci
FC
Bonan
Carla Denise
CD
Netto
Carlos Alexandre
CA
Wyse
Angela Terezinha de Souza
AT
D000110:Acetylcholinesterase / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology; D004789:Enzyme Activation / Q000502:physiology; D004967:Estrogens / Q000172:deficiency; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D006624:Hippocampus / Q000033:anatomy & histology / Q000378:metabolism; D006868:Hydrolysis; D010052:Ovariectomy; D010053:Ovary / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology*; D017698:Postmenopause / Q000502:physiology; D017729:Presynaptic Terminals / Q000378:metabolism; D016548:Prosencephalon / Q000033:anatomy & histology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000227:Adenine Nucleotides; D015415:Biomarkers; D004967:Estrogens; D012739:Gonadal Steroid Hormones; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000110:Acetylcholinesterase; D000254:Sodium-Potassium-Exchanging ATPase