Identification and mapping of ten new potential insulators in the FXYD5-COX7A1 region of human chromosome 19q13.12.
A positive-negative selection system revealed 10 potential insulators able to block enhancer interaction with promoter in the 10(6) bp human chromosome 19 region between genes FXYD5 and COX7A1. Relative positions of insulators and genes are in accord with the hypothesis that insulators subdivide genomic DNA into independently regulated loop domains.
Didych
D A
DA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Akopov
S B
SB
Snezhkov
E V
EV
Skaptsova
N V
NV
Nikolaev
L G
LG
Sverdlov
E D
ED
D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D006801:Humans; D038101:Insulator Elements; D007473:Ion Channels; D008562:Membrane Glycoproteins / Q000235:genetics*; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic
C451410:FXYD5 protein, human; D007473:Ion Channels; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; C513901:COX7A1 protein, human; D003576:Electron Transport Complex IV