Role of Cybr, a cytohesin binder and regulator, in CD4(+) T-cell function and host immunity.
Cytohesin binder and regulator (Cybr) is known to regulate leukocyte adhesion and migration. However, its function in T-cells is poorly understood. Here, we investigated the role of Cybr in CD4(+) T-cell function and host immunity. Cybr inhibited p38 phosphorylation following CD4(+) T-cell stimulation. Since p38 regulates the expression of T-box expressed in T-cells (T-bet) but not GATA binding protein 3 (GATA-3) in T-cells, Cybr decreased the expression of T-bet and IFN-gamma in CD4(+) T-cells. Moreover, we found that host immunity against Listeria infection and IFN-gamma production in blood were significantly compromised in Cybr-overexpressing transgenic mice. In summary, our data suggest that Cybr represses the expression of T-bet and IFN-gamma via an inhibition of p38 in T-cells and consequently reduces host resistance to bacterial infection in mice.
Chang
Shu-Lin
SL
Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei, Taiwan.
Chang
C Lee-Tian
CL
Huang
Pei-Ing
PI
Tao
Mi-Hua
MH
Yang
Wen-Chin
WC
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology* / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000096:biosynthesis / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000097:blood / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation / Q000276:immunology; D020825:T-Box Domain Proteins / Q000096:biosynthesis / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology / Q000378:metabolism; D000098815:T-bet Transcription Factor
D002352:Carrier Proteins; C513660:Cybr protein, mouse; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D008565:Membrane Proteins; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D007371:Interferon-gamma; D048051:p38 Mitogen-Activated Protein Kinases