title: The Staphylococcus aureus lineage-specific markers collagen adhesin and toxic shock syndrome toxin 1 distinguish multilocus sequence typing clonal complexes within spa clonal complexes.
abstract: Spa typing/based upon repeat pattern (BURP) sometimes cannot differentiate multilocus sequence typing (MLST) clonal complexes (CCs) within spa-CCs. It has been observed previously that virulence factors, such as collagen adhesin (CNA) and toxic shock syndrome toxin 1 (TSST-1), are associated with certain Staphylococcus aureus lineages. Analysis of methicillin-sensitive and methicillin-resistant S. aureus by spa typing/BURP and detection of CNA and TSST-1 observed an association between CNA and MLST CC1, 12, 22, 30, 45, 51, and 239 and between TSST-1 and MLST CC30. In spa-CC 012, associated with MLST CC7, CC15, and CC30, MLST CC30 could be distinguished from MLST CC7 and CC15 with CNA and TSST-1 as lineage-specific markers. Lineage-specific markers can overcome clustering of nonrelated MLST CCs into 1 spa-CC.
authors.affiliation: Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands. ruud.deurenberg@mumc.nl
authors.forename: Ruud H
authors.initials: RH
authors.lastname: Deurenberg
authors.forename: Michelle I A
authors.initials: MI
authors.lastname: Rijnders
authors.forename: Silvie
authors.initials: S
authors.lastname: Sebastian
authors.forename: Maaike A
authors.initials: MA
authors.lastname: Welling
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Beisser
authors.forename: Ellen E
authors.initials: EE
authors.lastname: Stobberingh
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics*; D018089:Superantigens / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D018089:Superantigens; C048165:adhesin, Staphylococcus aureus; C030107:enterotoxin F, Staphylococcal