False-positive PCR detection of Tropheryma whipplei in cerebrospinal fluid and biopsy samples from a child with chronic lymphocytic meningitis.
We report the case of a teenager with chronic lymphocytic meningitis for whom Tropheryma whipplei 16S rRNA PCR results were positive in two cerebrospinal fluid samples and one duodenal biopsy specimen. PCR targeting another specific sequence of Tropheryma whipplei and sequencing of the initially amplified 16S rRNA fragment did not confirm the results.
Goyo
Daniel
D
Servicio de Microbiología, Hospital Universitario 12 de Octubre, Avenida de Córdoba sn, Madrid 28041, Spain. fchaves.hdoc@salud.madrid.org
Camacho
Ana
A
Gómez
Carmen
C
de Las Heras
Rogelio Simón
RS
Otero
Joaquín R
JR
Chaves
Fernando
F
D000293:Adolescent; D001483:Base Sequence; D001706:Biopsy; D002555:Cerebrospinal Fluid / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004386:Duodenum / Q000382:microbiology; D005189:False Positive Reactions; D006801:Humans; D008297:Male; D016920:Meningitis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D054851:Tropheryma / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S