title: [Acetylation of hepatic histone H3 in rats with intrauterine growth retardation].
abstract: OBJECTIVE
  To investigate the effects of intrauterine growth retardation (IUGR) caused by malnutrition during pregnancy on the acetylation of histone H3 and expression of histonedeacetylase1(HDAC1) in the hepar of the adult offspring and to explore the relationship between them.
  
  
  METHODS
  Male 8-week-old offspring from maternal protein-malnutrition dams were studied. The expression of HDAC1 mRNA in the hepar was measured by fluorescent quantization RT-PCR. The levels of hepatic nuclear HDAC1 protein and acetylation of histone H3/K9 were assessed by Western blot.
  
  
  RESULTS
  The hepatic HDAC1 mRNA expression in IUGR rats was reduced to 54% of that of normal control rats (t=2.042, p<0.05). A decline in nuclear expression of HDAC1 protein (438 +/- 47) was also noted when compared with normal controls (1,128 +/- 110) (t=2.179, p<0.05). In contrast, the percentage of acetylated histone H3/K9 in IUGR rats (17.3 +/- 1.6%) increased significantly compared with that of normal control rats (10.5 +/- 1.2%) (t=3.597, p<0.01). The level of acetylated histone H3/K9 was negatively correlated with the HDAC1 protein concentration (r=-0.781, p<0.01).
  
  
  CONCLUSIONS
  Increased hepatic acetylation of histone H3 in the IUGR offspring might be caused by decreased HDAC1 expression in nuclear protein. This may contribute to the transcription change of some genes in the hepar.
authors.affiliation: Experimental Research Center, Shengjing Hospital of China Medical University, Shenyang 110004, China. liuxm1@sj-hospital.org
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Hai-Lan
authors.initials: HL
authors.lastname: Zhang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Song
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Jiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000107:Acetylation; D000818:Animals; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D056284:Histone Deacetylase 1 / Q000032:analysis / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006657:Histones; D012333:RNA, Messenger; C515890:Hdac1 protein, rat; D056284:Histone Deacetylase 1