Novel GH10 xylanase, with a fibronectin type 3 domain, from Cellulosimicrobium sp. strain HY-13, a bacterium in the gut of Eisenia fetida.
The gene encoding a novel modular xylanase from Cellulosimicrobium sp. strain HY-13 was identified and expressed in Escherichia coli, and its truncated gene product was characterized. The enzyme consisted of three distinct functional domains, an N-terminal catalytic GH10 domain, a fibronectin type 3 domain, and C-terminal carbohydrate-binding module 2.
Kim
Do Young
DY
Industrial Bio-Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea
Han
Mi Kyoung
MK
Park
Doo-Sang
DS
Lee
Jong Suk
JS
Oh
Hyun-Woo
HW
Shin
Dong-Ha
DH
Jeong
Tae-Sook
TS
Kim
Sung Uk
SU
Bae
Kyung Sook
KS
Son
Kwang-Hee
KH
Park
Ho-Yong
HY
D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000382:microbiology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013379:Substrate Specificity / Q000235:genetics; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D005353:Fibronectins; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases