Convenient modular method for affinity labeling (MoAL method) based on a catalytic amidation.
A modular methodology for affinity labeling, in which three essential elements generally constituting affinity probes are prepared separately as individual molecules, has been developed based on a catalytic amidation.
Kunishima
Munetaka
M
Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical, and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. kunisima@p.kanazawa-u.ac.jp
Nakanishi
Shuichi
S
Nishida
Jin
J
Tanaka
Hiroyuki
H
Morisaki
Daiki
D
Hioki
Kazuhito
K
Nomoto
Hiroshi
H
D000345:Affinity Labels / Q000737:chemistry*; D000818:Animals; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002384:Catalysis; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D016716:PC12 Cells; D011506:Proteins / Q000737:chemistry*; D051381:Rats
D000345:Affinity Labels; D005456:Fluorescent Dyes; D011506:Proteins; D001360:Avidin; D001710:Biotin