title: Enterotoxigenic coagulase positive Staphylococcus in milk and milk products, lben and jben, in northern Morocco.
abstract: BACKGROUND
  The aim of this research was to determine the prevalence of enterotoxin genes (sea-seo) in Coagulase Positive Staphylococcus (CPS) isolated from unpasteurized milk and milk products. These results were compared with the results obtained by using the detection kit SET-RPLA for the specific detection of staphylococcal enterotoxins (SEA-SED).
  
  
  METHODS
  Eighty-one samples of milk and milk products were analyzed for the presence of Staphylococcus strains. Forty-six coagulase positive Staphylococcus isolates were tested for the production of staphylococcal enterotoxins (SEA-SED) by using the reversed passive latex agglutination method. The strains were also tested for the presence of se genes (sea-seo) by polymerase chain reaction.
  
  
  RESULTS
  One or more classical enterotoxin products (SEA-SED) were observed in 39% of the strains tested, while se genes were detected in 56.5%. SEA and sea were most commonly detected. For newly discovered se genes among CPS isolates tested in this study, except the seh gene which was revealed in four isolates (8.7 %), none of the strains harbored any of the other se genes (see, seg, sei, sej, sek, sel, sem, seo and sen).
  
  
  CONCLUSIONS
  The finding of a pathogen such as staphylococci-producing SEs and containing se genes in milk and milk products in northern Morocco may indicate a problem for public health in this region. The presence of enterotoxigenic strains in food does not always necessarily mean that the toxin will be produced. For that reason, the combination of both methods (RPLA and PCR) is a guarantee for success in diagnostic analysis tests.
authors.affiliation: Département de Sécurité Alimentaire & Environnementale, Institut Pasteur Du Maroc-Tanger-Maroc.
authors.forename: Abdrezzak
authors.initials: A
authors.lastname: Bendahou
authors.forename: Mohammed
authors.initials: M
authors.lastname: Abid
authors.forename: Nadine
authors.initials: N
authors.lastname: Bouteldoun
authors.forename: Dierick
authors.initials: D
authors.lastname: Catelejine
authors.forename: Mariam
authors.initials: M
authors.lastname: Lebbadi
mesh_terms: D000818:Animals; D003030:Coagulase / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000235:genetics; D003611:Dairy Products / Q000382:microbiology*; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D007841:Latex Fixation Tests / Q000379:methods; D008892:Milk / Q000382:microbiology*; D009018:Morocco; D016133:Polymerase Chain Reaction / Q000379:methods; D013211:Staphylococcus aureus / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D003030:Coagulase; D004269:DNA, Bacterial; D004768:Enterotoxins