[Effect and mechanism of matrine on adhesion and invasion of salivary gland adenoid cystic carcinoma cells in vitro].
OBJECTIVE
To investigate the effect and mechanism of matrine on adhesion and invasion of human adenoid cystic carcinoma ACC-M cells in vitro.


METHODS
MTT assay was used to examine the effect of matrine on proliferation of ACC-M cells treated after 1 day to 3 days. Byden chamber assay was performed to detect the effect of matrine on invasion capacity of the cells; Effect on adhesion potential of ACC-M cells was tested by cell matrigel adhesion assay. Mechanism of inhibitory adhesion and invasion was investigated by expression of E-cad protein. The results was analyzed by SPSS11.0 software package.


RESULTS
Matrine could inhibit the proliferation of ACC-M cell lines with apparent dose-dependent and time-dependent effect. Matrine significantly inhibited adhesion and invasion capacity of ACC-M cell lines in vitro with concentration increasing. The expression level of E-cad was significantly increased compared with the untreated group in ACC-M cell lines.


CONCLUSIONS
Matrine can inhibit the adhesion and invasion capacity of ACC-M cell lines in vitro. Mechanism of the inhibition effect may be related to over expression of E-cad protein.
Zhao
Jun-fang
JF
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province,China. junfangzhao@zzu.edu.cn
Li
Xin-ming
XM
Xie
Wei-hong
WH
Wang
Hai-bin
HB
Fang
Zheng
Z
Li
Qing-chuan
QC
D000470:Alkaloids; D003528:Carcinoma, Adenoid Cystic; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D006801:Humans; D066298:In Vitro Techniques; D011807:Quinolizines; D012468:Salivary Gland Neoplasms; D012469:Salivary Glands; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D000093842:Matrines