title: Differences in collagen architecture between keloid, hypertrophic scar, normotrophic scar, and normal skin: An objective histopathological analysis.
abstract: Normotrophic, hypertrophic, and keloidal scars are different types of scar formation, which all need a different approach in treatment. Therefore, it is important to differentiate between these types of scar, not only clinically but also histopathologically. Differences were explored for collagen orientation and bundle thickness in 25 normal skin, 57 normotrophic scar, 56 hypertrophic scar, and 56 keloid biopsies, which were selected on clinical diagnosis. Image analysis was performed by fast fourier transformation. The calculated collagen orientation index ranged from 0 (random orientation) to 1 (parallel orientation). The bundle distance was calculated by the average distance between the centers of the collagen bundles. The results showed that compared with all three types of scars, the collagen orientation index was significantly lower in normal skin, which indicates that scars are organized in a more parallel manner. No differences were found between the different scars. Secondly, compared with normal skin, normotrophic scar, and hypertrophic scar, the bundle distance was significantly larger in keloidal scar, which suggests that thicker collagen bundles are present in keloidal scar. This first extensive histological study showed objective differences between normal skin, normotrophic, hypertrophic, and keloidal scar.
authors.affiliation: Association of Dutch Burn Centres Beverwijk, the Netherlands.
authors.forename: Pauline D H M
authors.initials: PD
authors.lastname: Verhaegen
authors.forename: Paul P M
authors.initials: PP
authors.lastname: van Zuijlen
authors.forename: Noor M
authors.initials: NM
authors.lastname: Pennings
authors.forename: Jan
authors.initials: J
authors.lastname: van Marle
authors.forename: Frank B
authors.initials: FB
authors.lastname: Niessen
authors.forename: Chantal M A M
authors.initials: CM
authors.lastname: van der Horst
authors.forename: Esther
authors.initials: E
authors.lastname: Middelkoop
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002921:Cicatrix / Q000473:pathology*; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D003094:Collagen / Q000032:analysis / Q000502:physiology*; D005260:Female; D006801:Humans; D007627:Keloid / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000737:chemistry / Q000473:pathology*; D014945:Wound Healing / Q000502:physiology*; D055815:Young Adult
chemical_list: D003094:Collagen