title: Reversible effect of magnetic fields on human lymphocyte activation patterns: different sensitivity of naive and memory lymphocyte subsets.
abstract: The aim of this study was to investigate the influence of 50 Hz magnetic or static magnetic fields of 0.5 mT on subsets of human CD4(+) T cells in terms of cytokine release/content, cell proliferation and intracellular free calcium concentration. CD4(+) T cells can be divided into different subsets on the basis of surface marker expression, such as CD45, and T cells can be divided into naive (CD45RA(+)) and memory (CD45RA(-)) cells. In this study, the effects of magnetic fields after 24 and 48 h of cell culture were analyzed. We found that the CD4(+)CD45RA(-) T subset were more sensitive after 2 h of exposure. Decreases in the release/content of IFN-gamma, in cell proliferation and in intracellular free calcium concentrations were observed in exposed CD4(+)CD45RA(-) T cells compared to CD4(+)CD45RA(+) T cells. The results suggest that exposure to the magnetic fields induces a delay in the response to stimulants and that modifications are rapidly reversible, at least after a short exposure.
authors.affiliation: Dipartimento di Biotecnologie Mediche e Medicina Legale, Università di Palermo, Palermo, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Salerno
authors.forename: Carmela
authors.initials: C
authors.lastname: La Mendola
authors.forename: Marco Pio
authors.initials: MP
authors.lastname: La Manna
authors.forename: Antonio
authors.initials: A
authors.lastname: Lo Casto
authors.forename: Nadia
authors.initials: N
authors.lastname: Caccamo
authors.forename: Alfredo
authors.initials: A
authors.lastname: Salerno
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology* / Q000528:radiation effects; D007371:Interferon-gamma / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation / Q000528:radiation effects; D008280:Magnetics; D008297:Male; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors
chemical_list: D007371:Interferon-gamma; D017493:Leukocyte Common Antigens; D002118:Calcium