[Effects of CO2 and 5% dextrose solution on expression of CD44 and ICAM-1 in endometrial carcinoma cells].
OBJECTIVE
To investigate the effects of 5% dextrose solution and different CO2 pressures on expression of adhesion molecules (including CD44 and ICAM-1) of endometrial carcinoma cells in vitro. To provide experimental base for the safety question of hysteroscope operation and the selection of better distending medium for the patients with suspected endometrial cancer.


METHODS
Endometrial cancer cells (HEC-1-B) were put in 5% dextrose, 70 mmHg 100% CO2, 100 mmHg 100% CO2 at 37 degrees C, the control group was not given any administration, respectively for 1 hour. Expression of CD44 and ICAM-1 were measured at 0, 12, 24, 48 and 72 hours by using flow cytometry.


RESULTS
The expression of CD44 and ICAM-1 increased significantly at 0 h-72 h after the cells were exposed to 5% dextrose, 70 mmHg 100% CO2, 100 mmHg 100% CO2 for 1 hour (P<0.05) respectively, and returned to the control levels by 72 h. With the increase of the CO2 pressure, the expression of the CD44 and ICAM-1 increased (P<0.05). The expression of the CD44 and ICAM-1 totally increased significantly compared with control.


CONCLUSIONS
Both high pressure CO2 (100 mmHg) and 5% dextrose solution can increase the expression of CD44 and ICAM-1 in endometrial cancer cells, and may promote the capability of adhesion of endometrial cancer cells. Lower CO2 pressure does not change the expression of CD44 and ICAM-1 in endometrial cancer cells, therefore the low CO2 pressure (70 mmHg) is safer as distending medium for the patients with suspected endometrial cancer.
Liao
Zhi
Z
Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Xiao
Hong-Tao
HT
Li
Li
L
Wang
Yu-Jue
YJ
Qu
Da-Cheng
DC
Gao
Xue-Mei
XM
D002245:Carbon Dioxide / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D009362:Neoplasm Metastasis
D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1; D002245:Carbon Dioxide; D005947:Glucose