Altered profiles of gene expression in curcumin-treated rats with experimentally induced myocardial infarction.
Curcumin has extensive cardioprotective effects against diabetic cardiovascular complications, cardiac hypertrophy and myocardial infarction (MI), but the molecular mechanism behind such cardioprotective effects remains still unclear. To explore the mechanism of MI, a rat model of coronary artery ligation was used to assess the cardioprotective effects of curcumin. Microarray technology was employed to detect the gene expression in the heart of MI rats treated with curcumin. Semiquantitative RT-PCR was then performed to verify the microarray result. Our results showed that curcumin could improve heart function, diminish infarct size and reverse the abnormal changes in the activities of serum lactate dehydrogenase and creatine kinase MB in rats after MI. A total of 179 genes were found to be significantly differentially expressed between sham-operated rats and coronary artery-ligated rats. Cytokine-cytokine receptor interaction, ECM-receptor interaction, focal adhesions and colorectal cancer pathway may be involved in the cardioprotective effects of curcumin.
Hong
Dongsheng
D
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
Zeng
Xiaowei
X
Xu
Wei
W
Ma
Jing
J
Tong
Yinghui
Y
Chen
Yan
Y
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002316:Cardiotonic Agents / Q000494:pharmacology*; D052279:Creatine Kinase, MB Form / Q000097:blood; D003474:Curcumin / Q000494:pharmacology*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016277:Ventricular Function, Left / Q000187:drug effects; D017725:Ventricular Pressure / Q000187:drug effects
D015415:Biomarkers; D002316:Cardiotonic Agents; D007770:L-Lactate Dehydrogenase; D052279:Creatine Kinase, MB Form; D003474:Curcumin