[Effect of Mycobacterium smegmatis vaccine on the level of nitric oxide produced by peritoneal macrophages in mice].
OBJECTIVE
To study the effect of Mycobacterium smegmatis vaccine on the level of nitric oxide (NO) produced by peritoneal macrophages in immunized mice.


METHODS
Balb/c mice were randomized into low-dose, middle-dose, and high-dose groups (injected with different doses of Mycobacterium smegmatis vaccine) and a control group (injected with normal saline). Then the peritoneal macrophages were cultured with lipopolysaccharide in vitro. The supernatants were collected and the concentrations of NO were analyzed through the reaction with Griess reagents.


RESULTS
The levels of NO produced by the peritoneal macrophages in the control group, low-dose group, middle-dose group, and high-dose group were (3.50 +/- 3.11), (16.63 +/- 6.47), (13.97 +/- 6.20), and (7.55 +/- 2.26) ng/ml, respectively. The levels of NO in all dosing groups were significantly different from that in control group (P < 0.01).


CONCLUSIONS
Mycobacterium smegmatis vaccine can promote the peritoneal macrophages to produce NO in mice.
Xu
Miao
M
1st Bacterial Vaccine Division, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Chen
Bao-Wen
BW
Shen
Xiao-Bing
XB
Su
Cheng
C
Wang
Guo-Zhi
GZ
D000818:Animals; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D008070:Lipopolysaccharides; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020102:Mycobacterium smegmatis; D009569:Nitric Oxide / Q000378:metabolism*
D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D009569:Nitric Oxide