Impact of a recombinant fowlpox vaccine on the efficacy of adoptive cell therapy with tumor infiltrating lymphocytes in a patient with metastatic melanoma.
A patient with metastatic melanoma who had progressive disease after prior surgical resections, high dose interleukin-2, and anti-cytotoxic T lymphocyte antigen-4 antibody received sequential treatments with autologous tumor infiltrating lymphocytes that recognized the gp100 melanocyte differentiation antigen. Although no clinical response was seen when cells were administered alone, an objective clinical response to therapy was seen with tumor infiltrating lymphocytes administered together with a highly immunogenic fowlpox vaccine expressing a gp100: 209-217 (210M) epitope. Persistence of the transferred antigen-specific lymphocytes in the peripheral blood was observed only after adoptive cell therapy plus administration of vaccine. Cell proliferation in vitro was further stimulated by additional vaccine and interleukin-2. The patient has an ongoing partial response at 10 months after the last treatment.
Smith
Franz O
FO
Surgery Branch, National Cancer Institute, National Institute of Health, Bethesda, MD 20892-1201, USA.
Klapper
Jacob A
JA
Wunderlich
John R
JR
Rosenberg
Steven A
SA
Dudley
Mark E
ME
D001804:Blood Transfusion, Autologous; D049109:Cell Proliferation; D018450:Disease Progression; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000378:metabolism; D005153:Facial Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D005587:Fowlpox virus; D006801:Humans; D016219:Immunotherapy, Adoptive; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009362:Neoplasm Metastasis; D014765:Viral Vaccines / Q000235:genetics; D058951:gp100 Melanoma Antigen
D018984:Epitopes, T-Lymphocyte; D008562:Membrane Glycoproteins; C548637:PMEL protein, human; D014765:Viral Vaccines; D058951:gp100 Melanoma Antigen