Ocular prostaglandin production and morphology in mice lacking a single isoform of cyclooxygenase.
Prostaglandins have many important roles in ocular physiology and are used clinically for the treatment of glaucoma. The aim of this study was to analyse the contribution of each cyclooxygenase isoform to ocular prostaglandin production using isoform-specific knockout mice. Ex vivo PGE(2), 6-keto-PGF(1alpha), and TXB(2) production was measured from whole eyes, corneal tissue, uveoscleral tissue, lens, retina and optic nerve using enzyme-linked immunosorbant assays. Ocular immunohistochemical and histological analysis was also conducted for each genotype. Levels of each of the prostaglandins measured were significantly decreased in the corneal tissue, uveoscleral tissue, lens, retina and optic nerve of COX-1(-/-) mice in comparison with wild-type mice. In contrast, COX-2(-/-) mice had similar levels of ocular prostaglandin production to wild-type mice. These results suggest that COX-1 is the principal isoform responsible for prostaglandin production in the mouse eye. The absence of COX-1 or COX-2 did not appear to effect ocular development in these mice.
Cryan
Lorna M
LM
Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, Dublin, Ireland. lorna.cryan@ucd.ie
Fitzgerald
Desmond J
DJ
O'Brien
Colm
C
D000367:Age Factors; D000818:Animals; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005123:Eye / Q000033:anatomy & histology* / Q000254:growth & development / Q000378:metabolism*; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008954:Models, Biological; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D011453:Prostaglandins / Q000378:metabolism*; D014018:Tissue Distribution
D007527:Isoenzymes; D008565:Membrane Proteins; D011453:Prostaglandins; C077623:Ptgs2 protein, mouse; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496512:Ptgs1 protein, mouse