Complementary peptide sequence coverage using alternative enzymes for on-line digestion with a triaxial electrospray probe.
Using alternative enzymes for on-line digestion with a triaxial electrospray probe extends sequence coverage. This is the first report of utilization of our triaxial probe for on-line analysis with enzymes other than pepsin, suggesting potential for broader application. The probe allows access to processes occurring on a timescale and/or involving substrate conformations complementary to those for conventional (off-line) digestion. Some of the features observed in application to Abeta fibrils are suggestive of unique reactive intermediates during dissolution. Data obtained with enzyme mixtures suggest synergistic effects.
Dykstra
Andrew B
AB
Department of Chemistry, University of Tennessee, Knoxville, Tennessee, USA.
Chen
Maolian
M
Cook
Kelsey D
KD
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry*; D006860:Hydrogen Bonding; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009862:Online Systems; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000145:classification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D010447:Peptide Hydrolases; D016282:Aspartic Acid Endopeptidases; C042466:Endothia aspartic proteinase; D010434:Pepsin A