Determinants of DNA yield and purity collected with buccal cell samples.
Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 microg (median 54.3 microg; mean 82.2 microg +/- SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 microg) than those from women (median 44.2 microg). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics.
van Wieren-de Wijer
D B M A
DB
Department of Pharmacoepidemiology & Pharmacotherapy, Faculty of Science, Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, P. O. Box 80082, 3508 TB, Utrecht, The Netherlands. B.M.A.vanWieren@uu.nl
Maitland-van der Zee
A H
AH
de Boer
A
A
Belitser
S V
SV
Kroon
A A
AA
de Leeuw
P W
PW
Schiffers
P
P
Janssen
R G J H
RG
van Duijn
C M
CM
Stricker
B H C H
BH
Klungel
O H
OH
D000367:Age Factors; D000368:Aged; D000959:Antihypertensive Agents / Q000009:adverse effects; D016022:Case-Control Studies; D004247:DNA / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology* / Q000187:drug effects; D012737:Sex Factors; D013048:Specimen Handling
D000959:Antihypertensive Agents; D004247:DNA