Short communication: evidence for the presence of a putative odorant-binding protein in bovine colostrum.
Using a combination of PAGE and mass spectrometry for protein identification, we obtained evidence that a putative odorant-binding protein, designated hypothetical protein LOC517854, occurs in bovine colostrum. This protein, termed as a putative bovine colostral odorant-binding protein (bcOBP), consists of 172 AA residues, including a putative 16-AA signal peptide. The theoretical isoelectric point value and molecular mass of the full-length sequence of bcOBP were calculated to be 4.57 and 19604.18, respectively. The highest sequence similarity (83%) was observed with a potential pheromone transporter, Allergen Bos d 2. An odorant-binding protein derived from bovine nasal mucosa showed relatively low sequence similarity (52%) against bcOBP. Its biological function is unclear, but pheromone transport could be considered. This is the first report of a putative odorant-binding protein in bovine colostrum.
Fukuda
K
K
Department of Animal and Food Hygiene, Obihiro University of agriculture and Veterinary medicine, Obihiro, Hokkaido 080-8555, Japan.
Senda
A
A
Ishii
T
T
Urashima
T
T
Morita
M
M
Terabayashi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D008969:Molecular Sequence Data; D009812:Odorants; D010675:Pheromones; D018035:Receptors, Odorant; D017385:Sequence Homology
D002352:Carrier Proteins; D010675:Pheromones; D018035:Receptors, Odorant