title: Protein and metabolite analysis reveals permanent induction of stress defense and cell regeneration processes in a tobacco cell suspension culture.
abstract: The secretome of a tobacco cell suspension culture was investigated by a combined proteomic and metabolomic approach. Protein analysis from 2-DE gels led to identification of 32 out of 60 spots from culture medium. Identified proteins were mainly involved in stress defence and cell regeneration processes. Among them three putative new isoforms, e.g. for chitinase, peroxidase and beta-1,4-xylosidase were identified, not yet present in available protein databases for the genus Nicotiana. GC-MS analysis of time course experiments revealed significant changes for metabolites involved in energy transport, signalling and cell development. Among them, the most significant increase was found for putrescine in the medium of cultures entering the exponential phase. Results showed strong abundance of stress associated proteins and metabolites in the absence of elicitors or additional stress treatments.
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
authors.forename: Rico
authors.initials: R
authors.lastname: Lippmann
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Kaspar
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
authors.forename: Twan
authors.initials: T
authors.lastname: Rutten
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Melzer
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
authors.forename: Jochen
authors.identifier: 0000-0001-7080-7983
authors.initials: J
authors.lastname: Kumlehn
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Matros
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
mesh_terms: D000595:Amino Acid Sequence; D048708:Cell Growth Processes / Q000235:genetics; D002460:Cell Line; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D055442:Metabolome; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D020543:Proteome; D010544:Peroxidases; D014995:Xylosidases; D002688:Chitinases; C026579:exo-1,4-beta-D-xylosidase; D011700:Putrescine