Fluorescence lifetime-resolved imaging.
This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.
Chen
Yi-Chun
YC
Bioengineering Department, University of Illinois at Urbana-Champaign, 3120 Digital Computer Laboratory, MC-278, 1304 West Springfield Avenue, Urbana, IL 61801, USA.
Clegg
Robert M
RM
D008856:Microscopy, Fluorescence / Q000379:methods*; D013223:Statistics as Topic; D013997:Time Factors