title: [Preparation technology of Zhangyanming dispersible tablet and determination of puerarin by HPLC].
abstract: OBJECTIVE
  To study the extraction and pharmceutics technology of Zhangyanming dispersible tablet and establish the method for determining the content of puerarin by HPLC.
  
  
  METHODS
  Using total extracted puerarin and disintegrating time as evaluation indexes and the conditions for determination were as follows: Hypersil BDS C18 column (5 microm, 4.6 mm x 200 mm), Methanol-H2O (25:75) as the mobile phase, the detection wavelength at 250 nm, the column temperature at 30 degrees C, and 1.0 mL/min as the mobile phase speed.
  
  
  RESULTS
  The calibration curve was linear at the range of 0.1699 to 1.3594 microg for the puerarin content and the linear equation was y = 3.054 x 10(6)x - 24019 (r = 0.9998). The average recovery ratio was 100.03%, and the relative standard deviation was 1.79%.
  
  
  CONCLUSIONS
  The new preparation technology is reasonable and HPLC can be used to determine the content of puerarin in Zhangyanming dispersible tablet because the operation was convenient, fast, easy to repeat, and the analysis results were accurate and especial.
authors.affiliation: Laboratory of Natural Drug, Institute of Materia Medica of Jiangxi Province, Nanchang 330029, China. zhucqin@sohu.com
authors.forename: Cai-Qing
authors.initials: CQ
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Wu
authors.initials: W
authors.lastname: Di
authors.forename: Nan-Sheng
authors.initials: NS
authors.lastname: He
authors.forename: Xian-Yi
authors.initials: XY
authors.lastname: Zeng
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007529:Isoflavones / Q000032:analysis*; D000432:Methanol / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012995:Solubility; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D013607:Tablets; D014867:Water; D000432:Methanol; C033607:puerarin