title: Classical swine fever virus Erns glycoprotein antagonizes induction of interferon-beta by double-stranded RNA.
abstract: Classical swine fever virus (CSFV) is capable of counteracting innate cellular antiviral responses by inhibiting type I interferon (IFN)-alpha/beta induction. A function associated with CSFV N(pro), with respect to the inhibition of IFN-beta production, has been clearly elucidated. In this study, we explored the role of CSFV E(rns) in IFN-beta induction by exogenous double-stranded (ds) RNA. Synthetic dsRNA (poly (IC)) was used as an exogenous stimulus to trigger IFN-beta induction. CSFV E(rns) inhibited IFN-beta promoter-driven luciferase activity induced by poly (IC) in different cell lines, and the inhibitory effect was dose-dependent. Moreover, E(rns) reduced IFN-beta mRNA synthesis and blocked IFN-alpha/beta production induced by poly (IC), suggesting that this inhibition occurs at the transcriptional level. Furthermore, E(rns) counteracted poly (IC)-mediated IFN-beta induction independent of its ribonuclease activity. In conclusion, CSFV E(rns) antagonizes extracellular dsRNA-mediated IFN-beta expression. These findings contribute to our understanding of the pathogenesis of CSFV.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Luo
authors.forename: Dawei
authors.initials: D
authors.lastname: Ling
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Wan
authors.forename: Chuyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Zishu
authors.initials: Z
authors.lastname: Pan
mesh_terms: D000818:Animals; D002460:Cell Line; D006691:Classical Swine Fever / Q000235:genetics / Q000276:immunology* / Q000821:virology; D006692:Classical Swine Fever Virus; D006801:Humans; D016899:Interferon-beta / Q000235:genetics* / Q000276:immunology; D011070:Poly I-C / Q000276:immunology; D011401:Promoter Regions, Genetic; D012330:RNA, Double-Stranded / Q000276:immunology*; D013552:Swine; D015533:Transcriptional Activation; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D012330:RNA, Double-Stranded; D014759:Viral Envelope Proteins; D016899:Interferon-beta; D011070:Poly I-C