title: Human cytosolic tRNase ZL can downregulate gene expression through miRNA.
abstract: A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including approximately 25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3' untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.
authors.affiliation: Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Niigata 956-8603, Japan.
authors.forename: Reyad A
authors.initials: RA
authors.lastname: Elbarbary
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takaku
authors.forename: Naoto
authors.initials: N
authors.lastname: Uchiumi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tamiya
authors.forename: Mayumi
authors.initials: M
authors.lastname: Abe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishida
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nashimoto
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D003600:Cytosol / Q000201:enzymology; D015536:Down-Regulation; D004722:Endoribonucleases / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009690:Nucleic Acid Conformation
chemical_list: C544749:MIRN103 microRNA, human; D035683:MicroRNAs; D004722:Endoribonucleases; C531011:tRNase Z, human