title: Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS.
abstract: The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD-HPLC-ESI-TOF-MS and IT-MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF-MS in order to obtain molecular formula and exact mass. Posterior analyses with IT-MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization modes with two optimized linear gradients. In positive mode, the two most representative anthocyanins and other compounds were identified whereas the phenolic fraction, hydroxycitric acid and its lactone were identified using the negative ionization mode.
authors.affiliation: Faculty of Sciences, Department of Analytical Chemistry, University of Granada, Granada, Spain.
authors.forename: Inmaculada C
authors.initials: IC
authors.lastname: Rodríguez-Medina
authors.forename: Raúl
authors.initials: R
authors.lastname: Beltrán-Debón
authors.forename: Vicente Micol
authors.initials: VM
authors.lastname: Molina
authors.forename: Carlos
authors.initials: C
authors.lastname: Alonso-Villaverde
authors.forename: Jorge
authors.initials: J
authors.lastname: Joven
authors.forename: Javier A
authors.initials: JA
authors.lastname: Menéndez
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura-Carretero
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernández-Gutiérrez
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002951:Citrates / Q000032:analysis; D031584:Hibiscus / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D014867:Water / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D002951:Citrates; D010636:Phenols; D010936:Plant Extracts; D014867:Water; C007999:hydroxycitric acid