Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelope protein CN54gp140 within novel rheologically structured vehicles elicits specific immune responses.
Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrix-forming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.
Curran
Rhonda M
RM
The School of Pharmacy, The Queen's University of Belfast, Belfast, BT9 7BL, Northern Ireland, UK. rhonda.curran@qub.ac.uk
Donnelly
Louise
L
Morrow
Ryan J
RJ
Fraser
Carol
C
Andrews
Gavin
G
Cranage
Martin
M
Malcolm
R Karl
RK
Shattock
Robin J
RJ
Woolfson
A David
AD
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D000282:Administration, Intravaginal; D000818:Animals; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005782:Gels; D015483:HIV Antibodies / Q000097:blood*; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D011817:Rabbits; D012212:Rheology; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
D016915:AIDS Vaccines; D005782:Gels; D015483:HIV Antibodies; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1