title: Genome-wide mapping of PiggyBac transposon integrations in primary human T cells.
abstract: The piggyBac transposon system represents a promising nonviral tool for gene delivery and discovery, and may also be of value for clinical gene therapy. PiggyBac is a highly efficient integrating vector that stably transfects (approximately 40%) of primary human T cells for potential adoptive immunotherapy applications. To evaluate the potential genotoxicity of piggyBac, we compared 228 integration sites in primary human T cells to integrations in 2 other human-derived cell lines (HEK293 and HeLa) and randomly simulated integrations into the human genome. Our results revealed distinct differences between cell types. PiggyBac had a nonrandom integration profile and a preference for transcriptional units (approximately 50% into RefSeq genes in all cell types), CpG islands (18% in T cells and 8% in other human cells), and transcriptional start sites (<5 kb, 16% to 20% in all cell types). PiggyBac also preferred TTAA but not AT-rich regions of the human genome. We evaluated the expression of mapped genes into which piggyBac integrated, and found selection of more active genes in primary human T cells compared with other human cell types, possibly due to concomitant T-cell activation during transposition. Importantly, we found that in comparison to what has been reported for gammaretroviral and human lenitviral vectors, piggyBac had decreased integration frequency into or within 50 kb of the transcriptional start sites of known proto-oncogenes. Hence the piggyBac nonviral gene delivery system seems to represent a promising gene transfer system for clinical applications using human T lymphocytes.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Galvan
authors.forename: Yozo
authors.initials: Y
authors.lastname: Nakazawa
authors.forename: Aparna
authors.initials: A
authors.lastname: Kaja
authors.forename: Claudia
authors.initials: C
authors.lastname: Kettlun
authors.forename: Laurence J N
authors.initials: LJ
authors.lastname: Cooper
authors.forename: Cliona M
authors.initials: CM
authors.lastname: Rooney
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Wilson
mesh_terms: D018899:CpG Islands / Q000235:genetics; D004251:DNA Transposable Elements; D017988:Gammaretrovirus; D005822:Genetic Vectors; D055106:Genome-Wide Association Study; D006367:HeLa Cells; D006801:Humans; D016219:Immunotherapy, Adoptive; D016086:Lentivirus; D009152:Mutagenicity Tests; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D024363:Transcription Initiation Site
chemical_list: D004251:DNA Transposable Elements