Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.
SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.
Haraguchi
Yuki
Y
Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, Narashino, Chiba 275-0016, Japan.
Kuwasako
Kanako
K
Muto
Yutaka
Y
Bessho
Yoshitaka
Y
Nishimoto
Madoka
M
Yokoyama
Shigeyuki
S
Kanai
Akio
A
Kawai
Gota
G
Sakamoto
Taiichi
T
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry; D052156:SELEX Aptamer Technique; D017423:Sequence Analysis, RNA; D018271:Signal Recognition Particle / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D018271:Signal Recognition Particle; D012313:RNA