title: Reduced high-density lipoprotein 2b in non-obese type 2 diabetic patients analysed by a microfluidic chip method in a case-control study.
abstract: BACKGROUND
  Alterations in high-density lipoprotein (HDL) subfractions, especially in the HDL2b subfraction, have been reported in type 2 diabetes mellitus (T2DM). However, new methods for convenient and reliable quantitation of HDL2b are yet to be developed.
  
  
  METHODS
  Thirty-eight patients with T2DM were enrolled and age-, sex- and body mass index (BMI)-matched controls were selected from the same population. A microfluidic chip method was employed to analyse serum HDL subfractions.
  
  
  RESULTS
  The microfluidic chip method revealed a significant reduction in HDL2b and its ratio to total HDL in T2DM patients. There was a reverse correlation for total HDL and HDL2b, and its ratio with triglycerides, homeostasis model assessment-insulin sensitivity index (HOMA-IS) and insulin resistance index (HOMA-IR).
  
  
  CONCLUSIONS
  We have shown a reduction of HDL2b and its ratio to total HDL by a novel chip method in T2DM patients. The significant correlation between HDL2b and HOMA-IS and HOMA-IR may have further predictive value in clinical utility.
authors.affiliation: Institute of Cardiovascular Sciences, Peking University, Beijing, China.
authors.forename: Xunde
authors.initials: X
authors.lastname: Xian
authors.forename: Yahong
authors.initials: Y
authors.lastname: Ma
authors.forename: Danhui D
authors.initials: DD
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
authors.forename: Odilo
authors.initials: O
authors.lastname: Mueller
authors.forename: Elain
authors.initials: E
authors.lastname: Chang
authors.forename: Yves
authors.initials: Y
authors.lastname: Konigshofer
authors.forename: Mark
authors.initials: M
authors.lastname: Van-Cleve
authors.forename: Jinkui
authors.initials: J
authors.lastname: Yang
authors.forename: George
authors.initials: G
authors.lastname: Liu
mesh_terms: D000368:Aged; D044466:Asian People; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006706:Homeostasis; D006801:Humans; D007333:Insulin Resistance; D008075:Lipoproteins, HDL / Q000097:blood; D053439:Lipoproteins, HDL2 / Q000097:blood*; D008297:Male; D046210:Microfluidic Analytical Techniques; D008875:Middle Aged
chemical_list: D001786:Blood Glucose; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2