Doppler OCT imaging of cytoplasm shuttle flow in Physarum polycephalum.
The Doppler optical coherence tomography technique was applied to image the oscillatory dynamics of protoplasm in the strands of the plasmodium of slime mould Physarum polycephalum. Radial contractions of the gel-like walls of the strands and the velocity distributions in the sol-like endoplasm streaming along the plasmodial strands are imaged. The motility inhibitor effect of carbon dioxide on the cytoplasm shuttle flow and strand-wall contraction is shown. The optical attenuation coefficient of cytoplasm is estimated.
Bykov
Alexander V
AV
University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, 90014 Oulu, Finland. bykov@ee.oulu.fi
Priezzhev
Alexander V
AV
Lauri
Janne
J
Myllylä
Risto
R
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism*; D008018:Life Cycle Stages; D016839:Physarum polycephalum / Q000166:cytology* / Q000254:growth & development; D012542:Scattering, Radiation; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*