[Establishment and evaluation of nitrate reductase combined with mycobacteriophage assay].
OBJECTIVE
To establish a rapid, inexpensive, and simple drug susceptibility test (DST) for Mycobacterium tuberculosis (M. tb) and evaluate its feasibility.


METHODS
We used nitrate reductase combined with mycobacteriophage assay (PhaB-NRA) to test 49 clinical M. tb isolates of, and the results were compared with those of PhaB-NRA and traditional absolute concentration method.


RESULTS
The sensitivity, specificity, and accuracy of PhaB-NRA for rifampicin were 89.1%, 91.67%, and 89.8%; on the contrary, those of isonicotinyl hydrazide were 86.21%, 90.0%, and 87.8%, respectively. The coincidence between PhaB-NRA and traditional assay were 0.746 for rifampicin and 0.750 for isonicotinyl hydrazide.


CONCLUSIONS
PhaB-NRA is an inexpensive, rapid, and simple DST method. It is a promising rapid screening technique for DST of M. tb.
Xing
Ai-Ying
AY
Laboratory of Molecular Biology for Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Liu
Zhong-Quan
ZQ
Jia
Hong-Yan
HY
Gu
Shu-Xiang
SX
Zhang
Zong-De
ZD
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009160:Mycobacteriophages / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D050901:Nitrate Reductase / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D050901:Nitrate Reductase; D012293:Rifampin