Cell-targeted self-assembled DNA nanostructures.
We present two strategies for attaching self-assembled DNA arrays to the surfaces of cells. Our first approach uses biotin-streptavidin interactions to bind DNA architectures to biotinylated cells. The second approach takes advantage of specific antibody-cell surface interactions, conjugated arrays and the subsequent binding to native epidermal growth factor receptors expressed on cancer cells. DNA array-cell surface interactions were visualized by fluorescence, confocal microscopy, and scanning electron microscopy. This novel application of DNA nanoarrays provides strategies to specifically label cell surfaces with micrometer-sized patches, bind cells onto a designed functionalized DNA scaffold, engineer cell/cell networks into microtissues, and deliver materials to cell surfaces.
Koyfman
Alexey Y
AY
Biomolecular Science and Engineering Program, University of California-Santa Barbara, Santa Barbara, California 93106-9510, USA.
Braun
Gary B
GB
Reich
Norbert O
NO
D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D001710:Biotin / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D066246:ErbB Receptors / Q000276:immunology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry
D000906:Antibodies; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D001710:Biotin; D019809:Streptavidin; D066246:ErbB Receptors