Promotion of strand invasion by utilizing entropically-favored PNA.
Invasion of peptide nucleic acid (PNA) is important to target a specific site in double-stranded DNA. However, it needs improvement in many ways such as sequence versatility and invasion efficiency. It is here reported that one 10-mer strand of alpha-lysine substituted peptide nucleic acid (PNA) efficiently invades double-stranded DNA (dsDNA) in the presence of single-strand binding protein (SSB) via Watson-Crick rule. According to the thermodynamic parameters in this study, highly efficient invasion was achieved by a favorable entropy change. The importance of preorganization of PNAs for efficient strand invasion is indicated.
Ishizuka
Takumi
T
Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.
Sforza
Stefano
S
Komiyama
Makoto
M
D004247:DNA / Q000737:chemistry*; D019277:Entropy; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013816:Thermodynamics
D020135:Peptide Nucleic Acids; D004247:DNA