Development of a new high performance liquid chromatography method for measurement of coenzyme Q10 in healthy blood plasma.
OBJECTIVE
To improve a new high performance liquid chromatography (HPLC) method for coenzyme Q10 (CoQ10) measurement in human plasma.


METHODS
We conducted this study in the Chemistry and Biochemistry Department, College of Medicine, Al-Nahrain University, Baghdad, Iraq from September 2004 to July 2005. Sixty healthy volunteers aged 28.51+/-7.30 years were participated in this study. All participants were non-smokers and under no any treatment for the last 3 days and fast for 12 hours before analysis. Coenzyme Q10 levels were measured by improved isocratic reversed phase HPLC technique after one-step ice-cold 1-propanol extraction step.


RESULTS
The ultra violet (UV) detection, developed HPLC method gives a good linearity range between 0.1-4.0 mg/L. The coefficients of variations (CVs%) of within-day precision for CoQ10 in human plasma were 0.2-3.90%. Analytical recoveries were 95.5-101.30%, whereas the average CV% of CoQ10 reproducibility was 0.959%.


CONCLUSIONS
A new improved rapid, precise, and sensitive HPLC method for the quantitation of CoQ10 is suitable for analysis of blood plasma samples.
Abdul-Rasheed
Omar F
OF
Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Al-Kadhmiya, PO Box 70027, Baghdad, Iraq. dr.omar_rasheed@yahoo.com
Farid
Yahya Y
YY
D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012016:Reference Values; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood
D014451:Ubiquinone; C024989:coenzyme Q10