title: An in vivo experimental model to assess furcal lesions as a result of perforation.
abstract: OBJECTIVE
  To design and validate a rat molar model of furcal perforation to allow investigation of the biological phenomena that follow and to explore its potential for evaluating repair materials under standardized conditions.
  
  
  METHODS
  Eighteen male Wistar rats were used. Surgical aseptic procedures were carried out in order to open the pulp chamber of a first molar tooth. A cavity was prepared on the floor of the pulp chamber using a (1/4) round bur that created a communication between the furcation and the periodontal tissues. Six animals for each time point were sacrificed on days 14, 21 and 28 to assay morphological changes at the furcation region of molars. Maxillary bone was processed, removed and sectioned. Cellular infiltration, collagen deposition and bone resorption were assessed by histological analysis. Cellularity in the lesion area was determined by morphometric analysis. Data were analysed using parametric Student's t-test.
  
  
  RESULTS
  A furcal perforation model was standardized in which both radiological outcome and periodontal tissue reactions could be assessed through evaluation of cellularity, osteoclast activity and collagen deposition. The morphometric analysis revealed a greater number of cells 21 day post-surgery when compared with 14 days.
  
  
  CONCLUSIONS
  This animal model was suitable for radiological and histological evaluation of the processes that accompany surgical furcal perforation.
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: M J B
authors.initials: MJ
authors.lastname: Silva
authors.forename: M V
authors.initials: MV
authors.lastname: Caliari
authors.forename: A P R
authors.initials: AP
authors.lastname: Sobrinho
authors.forename: L Q
authors.initials: LQ
authors.lastname: Vieira
authors.forename: R M E
authors.initials: RM
authors.lastname: Arantes
mesh_terms: D017607:Aluminum Compounds / Q000627:therapeutic use; D000539:Alveolar Process / Q000293:injuries / Q000473:pathology; D000818:Animals; D001862:Bone Resorption / Q000209:etiology / Q000473:pathology; D017610:Calcium Compounds / Q000627:therapeutic use; D003094:Collagen; D003782:Dental Pulp / Q000473:pathology; D003786:Dental Pulp Cavity / Q000293:injuries* / Q000473:pathology; D003804:Dentin / Q000473:pathology; D004195:Disease Models, Animal; D004338:Drug Combinations; D006097:Granulation Tissue / Q000473:pathology; D006171:Gutta-Percha / Q000627:therapeutic use; D008297:Male; D008437:Maxilla / Q000293:injuries / Q000473:pathology; D008963:Molar / Q000293:injuries / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000502:physiology; D009504:Neutrophils / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology; D010087:Oxides / Q000627:therapeutic use; D010519:Periodontium / Q000293:injuries / Q000473:pathology; D011670:Pulpectomy; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000009:adverse effects* / Q000295:instrumentation; D017640:Silicates / Q000627:therapeutic use; D014945:Wound Healing / Q000502:physiology
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D006171:Gutta-Percha; D003094:Collagen