title: Evaluation of microbial contamination of air in two haematology departments equipped with ventilation systems with different filtration devices.
abstract: BACKGROUND
  Nosocomial infections (NI) are above all due to health-care workers practices, but also the contamination of the environment could lead to their rise in health-care facilities. Introduction. In the last years, the incidence of NI has increased due to a substantial rise in the number of immuno-compromised patients. These patients are often gathered in hospital areas declared at "high risk" of infection such as Hematology and Bone Marrow Transplant ward. In this study, we evaluated microbial contamination of the air in two divisions with high risk patients, focusing on the validity of the air system with correlation to the presence or not of the HEPA absolute filters.
  
  
  METHODS
  An environmental surveillance study has been carried out in two Divisions of Haematology, in two different Hospitals. Investigations have been performed by sampling air and by analyzing bacterial and fungal growth on microbiology plates after an incubation period.
  
  
  RESULTS
  Unit A, without HEPA filters in the ventilation systems, showed a gradual increase in the bacterial load 20 and 60 days after cleaning of the ventilation system. Mycetes and Aspergilli were not present in basal conditions, at 20 or 60 days after decontamination. Unit B, equipped with HEPA filters placed at the inlet vents, showed extremely low values of the bacterial load either in basal conditions or upon inspection 60 days after cleaning. No mycetes were present.
  
  
  CONCLUSIONS
  From the results obtained, it was evident that following the cleaning operation, the quality of the air is excellent in both types of equipment, since no mycetes were present and the bacterial load was < 20 CFU/mc in all the sites tested. However, although in subsequent controls mycetes were absent in both types of equipment, a great difference in the suspended bacterial load was found: Unit B was close to sterility whereas in Unit A a progressive increase was observed.
authors.affiliation: Department of Health Sciences, San Martino Hospital, University of Genoa, Italy. paolo.crimi@hsanmartino.liguria.it
authors.forename: P
authors.initials: P
authors.lastname: Crimi
authors.forename: M
authors.initials: M
authors.lastname: Valgiusti
authors.forename: G
authors.initials: G
authors.lastname: Macrina
authors.forename: A
authors.initials: A
authors.lastname: Grieco
authors.forename: L
authors.initials: L
authors.lastname: Massone
authors.forename: A
authors.initials: A
authors.lastname: Ciucci
authors.forename: F
authors.initials: F
authors.lastname: Ansaldi
authors.forename: L
authors.initials: L
authors.lastname: Sticchi
authors.forename: L
authors.initials: L
authors.lastname: Sasso
authors.forename: S
authors.initials: S
authors.lastname: Del Buono
authors.forename: P
authors.initials: P
authors.lastname: Durando
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis* / Q000517:prevention & control; D015169:Colony Count, Microbial; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology / Q000517:prevention & control; D004784:Environmental Monitoring / Q000379:methods; D062665:Epidemiological Monitoring; D004866:Equipment Contamination; D005374:Filtration / Q000295:instrumentation*; D006405:Hematology; D006749:Hospital Design and Construction; D006757:Hospital Units; D006801:Humans; D016867:Immunocompromised Host; D015994:Incidence; D017053:Infection Control / Q000379:methods; D007558:Italy / Q000453:epidemiology; D008172:Lung Diseases, Fungal / Q000453:epidemiology / Q000517:prevention & control; D014691:Ventilation / Q000295:instrumentation / Q000592:standards