title: Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm.
abstract: Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8-oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage. In all samples examined, increased levels of 8-oxoguanine were present only in those spermatozoa with fragmented DNA, suggesting a link between both DNA damage types.
authors.affiliation: INIBIC-Complejo Hospitalario Universitario A Coruña, Unidad de Genética, Coruña, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Santiso
authors.forename: María
authors.initials: M
authors.lastname: Tamayo
authors.forename: Jaime
authors.initials: J
authors.lastname: Gosálvez
authors.forename: Marcos
authors.initials: M
authors.lastname: Meseguer
authors.forename: Nicolás
authors.initials: N
authors.lastname: Garrido
authors.forename: José Luis
authors.initials: JL
authors.lastname: Fernández
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D053938:DNA Fragmentation; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D002843:Chromatin; D011933:Reagent Kits, Diagnostic; C024829:8-hydroxyguanine; D006147:Guanine