title: ST2 gene expression is proliferation-dependent and its ligand, IL-33, induces inflammatory reaction in endothelial cells.
abstract: ST2 gene products that are members of IL-1 receptor family are expressed in various cells such as growth-stimulated fibroblasts and Th2 helper T-cells, and recently, IL-33, which belongs to IL-1 family, was identified as the ligand for ST2L, the receptor type product of the ST2 gene. Subsequently, IL-33 and ST2L have been reported to be involved in Th2 immunity and inflammation, however, their functions on non-immunological cells are still obscure. Among non-immunological adhesive cells, vascular endothelial cells were reported to express both ST2 gene products and IL-33, therefore, we investigated the expression manner of the ST2 gene in vascular endothelial cells and the effect of IL-33 on endothelial cells. ST2 gene was expressed in each of the vascular endothelial cell types tested, and the expression was growth-dependent and down-regulated when the cells were differentiated to form vascular structures on the extracellular membrane matrix. IL-33 scarcely affected the growth and tube formation of the endothelial cells, but induced IL-6 and IL-8 secretion from endothelial cells with the rapid activation of extracellular signal-regulated kinase (ERK) 1/2, so IL-33 is supposed to involve in inflammatory reaction of vascular endothelial cells through its receptor, ST2L.
authors.affiliation: Department of Ophthalmology, School of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Aoki
authors.forename: Morisada
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ozaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Takezako
authors.forename: Hiroto
authors.initials: H
authors.lastname: Obata
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ibaraki
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Tsuru
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Tominaga
authors.forename: Ken
authors.initials: K
authors.lastname: Yanagisawa
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins / Q000378:metabolism*; D008024:Ligands; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism
chemical_list: C079241:IL1RL1 protein, human; C506536:IL33 protein, human; D018836:Inflammation Mediators; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins; D008024:Ligands; D011956:Receptors, Cell Surface