Protective effects of melatonin in reduction of oxidative damage and immunosuppression induced by aflatoxin B1-contaminated diets in young chicks.
In the present study, the ability of aflatoxin B(1) (AFB(1) ) to induce endogenous oxidative damages and the possible ameliorating effects of dietary melatonin (MEL) were investigated. Newly hatched broiler chicks (n = 240) were fed aflatoxin-contaminated diets (0.5 or 1 mg kg(-1) diet) with or without MEL (40 mg kg(-1) bw) supplementation in diet for 40 days. AFB(1) resulted in a significant increase in lipid peroxidation (LPO) in the liver and erythrocytes accompanied with suppression of superoxide dismutase (SOD) and catalase (CAT) enzyme activities of erythrocytes. It also caused a significant reduction in levels of serum proteins and marked elevation in activities of serum transaminases. AFB(1) treatment also decreased both humoral and cell-mediated immune responses in growing chicks. Simultaneous administration of MEL with AFB(1) resulted in an obvious improvement in all the tested parameters. Long-term rather than short-term administration of MEL was more effective in rendering protection against AFB(1)-induced toxicity.
Sirajudeen
M
M
Division of Physiology and Reproduction, Central Avian Research Institute, Izatnagar, India. shiraz.m@rediff.com
Gopi
Kandasamy
K
Tyagi
Jagbir Singh
JS
Moudgal
Ram Phal
RP
Mohan
Jag
J
Singh
Ram
R
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D002645:Chickens / Q000378:metabolism; D004032:Diet; D004305:Dose-Response Relationship, Drug; D007107:Immune System / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000008:administration & dosage / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D011041:Poisoning / Q000188:drug therapy; D011042:Poisons / Q000633:toxicity*
D000975:Antioxidants; D011042:Poisons; D016604:Aflatoxin B1; D008550:Melatonin