Structural basis of EB1 effects on microtubule dynamics.
+TIPs (plus-end tracking proteins) are an increasing group of molecules that localize preferentially to the end of growing microtubules. +TIPs regulate microtubule dynamics and contribute to the organization of the microtubular network within the cell. Thus they participate in a wide range of cellular processes including cell division, motility and morphogenesis. EB1 (end-binding 1) is a highly conserved key member of the +TIP group that has been shown to modulate microtubule dynamics both in vitro and in cells. EB1 is involved in accurate chromosome segregation during mitosis and in the polarization of the microtubule cytoskeleton in migrating cells. Here, we review recent in vitro studies that have started to reveal a regulating activity of EB1, and its yeast orthologue Mal3p, on microtubule structure. In particular, we examine how EB1-mediated changes in the microtubule architecture may explain its effects on microtubule dynamics.
Coquelle
Frédéric M
FM
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6026, Institut Fédératif de Recherche 140, Génétique Fonctionnelle Agronomie et Santé, Université de Rennes 1, Campus de Beaulieu, 263, Avenue du Général Leclerc, Rennes, France. frederic.coquelle@univ-rennes1.fr
Vitre
Benjamin
B
Arnal
Isabelle
I
D000818:Animals; D016764:Cell Polarity; D020090:Chromosome Segregation; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D008958:Models, Molecular; D011487:Protein Conformation; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism
C405804:EB1 microtubule binding proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin