title: Tryptophan and iodothyronine transport interactions in HepG2 human hepatoma cells.
abstract: This study identifies interactions between transport of the aromatic amino acid L: -tryptophan (Trp) and thyroid hormones (TH) in HepG2 human hepatoma cells. The major portion of Trp uptake in HepG2 cells occurs via the NEM-sensitive amino acid transport System L2 (consistent with hepatic LAT3 expression), with a smaller aromatic-AA selective System T (MCT10) component. LAT3 and MCT10 mRNA were both detected in HepG2 cells. Uptake of TH does not involve System L2, but a significant portion of T(3) uptake is mediated by System T, alongside a taurocholate-sensitive organic anion transporter. T(4) uptake into HepG2 cells appears to be mediated principally by organic anion/monocarboxylate transporters, with smaller contributions by System T and receptor-mediated endocytosis. TH-Trp transport interactions in liver cells centre on System T which, due to a perivenous localisation alongside deiodinase 1, may impact on hepatic T(3) generation and release.
authors.affiliation: Division of Molecular Physiology, College of Life Sciences, James Black Centre, University of Dundee, Dundee, DD1 5EH, UK.
authors.forename: James W A
authors.initials: JW
authors.lastname: Ritchie
authors.forename: Peter Maving
authors.initials: PM
authors.lastname: Taylor
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D017931:DNA Primers; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D014284:Triiodothyronine / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D014284:Triiodothyronine; D014364:Tryptophan