[Studies on antibacterial constituents of Discocleidion rufescens (2)].
OBJECTIVE
To screen effective constituents of Discocleidion rufescens against bacteria.


METHODS
Compounds were isolated by bioassay-guided fractionation method with various chromatographic methods and their structures were determined by spectral analysis and chemical evidence.


RESULTS
Seven compounds were isolated from the Et2O and n-BuOH extracts and identified as 5,7-dihydroxy-4'-methoxy flavone (1), quercetin (2), apigenin-7-O-beta-D-glucopyranoside (3), apigenin-7-O-neohesperidoside (4), luteolin-7-O-neohesperidoside (5), gallic acid (6) and beta-daucosteol (7).


CONCLUSIONS
Compounds 1-6 were isolated from genus Discocleidion for the first time and exhibited certain inhibitory activity against Escherichia coli. Compound 6 also showed antibacterial activity against Staphylococus aureus.
Tian
Ye
Y
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Tang
Haifeng
H
Wang
Xiaojuan
X
Qiu
Feng
F
Xue
Gaijin
G
Li
Jun
J
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D019665:Euphorbiaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D010936:Plant Extracts