[Expression of HSP60, TLR4 and NF-kappaBp65 in the peripheral blood of patients with atopic dermatitis].
OBJECTIVE
To investigate the expressions of HSP60, TLR4 and NF-kappaBp65 in the peripheral blood of patients with atopic dermatitis (AD) and their roles in the pathogenesis of AD.


METHODS
RT-PCR, immunocytochemistry and ELISA were performed to measure the mRNA expressions of TLR4 and NF-kappaBp65 in the peripheral lymphocytes and monocytes, and the serum HSP60, respectively. A total of 17 patients with AD were recruited for the study, with 15 healthy people serving as controls.


RESULTS
The levels of HSP60, TLR4 mRNA and NF-kappaBp65 were significantly higher in the peripheral blood of the patients with AD than in the controls (P<0.05).


CONCLUSIONS
HSP60, TLR4 and NF-kappaBp65 are overexpressed in the peripheral blood of patients with AD, which might be associated with the pathogenesis of AD.
Gao
Ying
Y
Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu, China.
Guo
Zai-Pei
ZP
D000293:Adolescent; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000097:blood*; D002648:Child; D003876:Dermatitis, Atopic / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000097:blood; D051197:Toll-Like Receptor 4 / Q000097:blood* / Q000235:genetics; D051996:Transcription Factor RelA / Q000097:blood*; D055815:Young Adult
D018834:Chaperonin 60; D012333:RNA, Messenger; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA