Recombinant Sindbis virus vectors designed to express protective antigen of Bacillus anthracis protect animals from anthrax and display synergy with ciprofloxacin.
Recombinant Sindbis viruses were engineered to express alternative forms of the protective antigen (PA) of Bacillus anthracis. The recombinant viruses induced PA-specific immunoglobulin G and neutralizing antibodies in Swiss Webster mice. Vaccination with the recombinant viruses induced immunity that offered some protection from a lethal Ames strain spore challenge and synergized the protective effects of ciprofloxacin.
Thomas
John M
JM
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555-1070, USA. JohnM.Thomas@utsouthwestern.edu
Moen
Scott T
ST
Gnade
Bryan T
BT
Vargas-Inchaustegui
Diego A
DA
Foltz
Sheri M
SM
Suarez
Giovanni
G
Heidner
Hans W
HW
König
Rolf
R
Chopra
Ashok K
AK
Peterson
Johnny W
JW
D000818:Animals; D000881:Anthrax / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000235:genetics / Q000276:immunology*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001408:Bacillus anthracis / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D002939:Ciprofloxacin / Q000627:therapeutic use*; D005822:Genetic Vectors; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D012845:Sindbis Virus / Q000235:genetics* / Q000276:immunology; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D022122:Anthrax Vaccines; D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; C030325:anthrax toxin; D002939:Ciprofloxacin