Steroid hormones alter AMP hydrolysis in intact trophozoites of Trichomonas vaginalis.
Trichomonas vaginalis infection may be influenced by the vaginal concentrations of estrogens. We have investigated the effects of 17beta-estradiol and dehydroepiandrosterone sulfate (DHEAS) on the ecto-5'-nucleotidase activity in fresh clinical (VP60) and in long-term-grown (30236 ATCC) isolates of T. vaginalis. In vitro exposure to DHEAS and 17beta-estradiol did not induce any changes in adenosine monophosphate (AMP) hydrolysis in these isolates. The treatment of parasites in the presence of DHEAS (0.01-1.0 microM) for 2 h inhibited AMP hydrolysis in VP60 isolate, whereas there were no significant changes in nucleotide hydrolysis in the presence of 17beta-estradiol. DHEAS and 17beta-estradiol (0.01-1.0 microM) for 2 h inhibited AMP hydrolysis in 30236 isolate. The 12 treatment with 0.1 microM DHEAS inhibited AMP hydrolysis, whereas 17beta-estradiol did not alter the nucleotide hydrolysis in VP60 isolate. Our findings have shown that the complex effect of steroid hormones and their receptors on T. vaginalis may promote changes in ecto-5'-nucleotidase activity during exposure to these hormones.
Rückert
Caroline
C
Programa de Pós-Graduação em Biologia Celular e Molecular, Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 90619-900, Porto Alegre, RS, Brazil.
Stuepp
Cristiane dos Santos
Cdos S
Gottardi
Bárbara
B
Rosa
Jéssica
J
Cisilotto
Julia
J
Borges
Fernanda Pires
FP
Bogo
Maurício Reis
MR
Tasca
Tiana
T
De Carli
Geraldo Attilio
GA
Bonan
Carla Denise
CD
D000249:Adenosine Monophosphate / Q000378:metabolism*; D000818:Animals; D019314:Dehydroepiandrosterone Sulfate / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D006728:Hormones / Q000378:metabolism*; D006868:Hydrolysis; D009708:Nucleotidases / Q000037:antagonists & inhibitors*; D013256:Steroids / Q000378:metabolism*; D014246:Trichomonas vaginalis / Q000187:drug effects* / Q000378:metabolism; D053058:Trophozoites / Q000187:drug effects* / Q000378:metabolism*
D006728:Hormones; D013256:Steroids; D000249:Adenosine Monophosphate; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate; C019598:5'-adenosine monophosphate phosphohydrolase; D009708:Nucleotidases