Photo-controllable aptamer.
We have successfully developed a new method for photoregulation of G-quadruplex formation using cis-trans photoisomerization of the photochromic nucleobase (8FV)G. Our photo-controllable quadruplexes can be switched between a very stable quadruplex state and a non-structured state in a straightforward and reversible fashion. We also demonstrated reversibly control binding of a G-quadruplex aptamer to thrombin.
Ogasawara
Shinzi
S
RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan. o_shinji@riken.jp
Maeda
Mizuo
M
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000528:radiation effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D024202:Electrophoretic Mobility Shift Assay; D005449:Fluorenes / Q000737:chemistry* / Q000528:radiation effects; D054856:G-Quadruplexes; D007536:Isomerism; D055668:Photochemical Processes; D010777:Photochemistry / Q000379:methods
D052157:Aptamers, Nucleotide; D005449:Fluorenes; C081676:thrombin aptamer; D003849:Deoxyguanosine