Comment on "The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles".
Zhang et al. (Research Articles, 13 March 2009, p. 1448) reported that synaptic vesicles usually release neurotransmitter through a kiss-and-run mechanism occurring within 1 second but that full collapse of the vesicles becomes more prevalent with repeated stimuli. We report that the kinetics of vesicle retrieval do not change during a stimulus train, with endocytosis occurring in 10 to 15 seconds.
Granseth
Björn
B
Department of Clinical and Experimental Medicine, Division of Cell Biology, Linköping University, SE 581 83 Linköping, Sweden.
Odermatt
Benjamin
B
Royle
Stephen J
SJ
Lagnado
Leon
L
D000200:Action Potentials; D000818:Animals; D004558:Electric Stimulation; D004705:Endocytosis; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008561:Membrane Fusion; D018377:Neurotransmitter Agents / Q000378:metabolism*; D045663:Quantum Dots; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000502:physiology*
D018377:Neurotransmitter Agents; D049452:Green Fluorescent Proteins