N-terminal acetylation of the neuronal protein SNAP-25 is revealed by the SMI81 monoclonal antibody.
The monoclonal antibody SMI81 binds SNAP-25, a major player in neurotransmitter release, with high affinity and has previously been used to follow changes in the levels of this protein in neuropsychiatric disorders. We report here that the SMI81 epitope is present at the extreme N-terminus of SNAP-25 and, unusually, cannot be recognized when present as an internal sequence. Although it is known that SNAP-25 can be palmitoylated and phosphorylated in brain, we now reveal the existence of a third modification, acetylation of the N-terminus. This acetylation event greatly increases the efficiency of SMI81 antibody binding. We show that this highly specific antibody can be used for studying brain function in many vertebrate organisms.
Connell
Emma
E
MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
Darios
Frédéric
F
Peak-Chew
Sew
S
Soloviev
Mikhail
M
Davletov
Bazbek
B
D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D017124:Conserved Sequence; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000378:metabolism*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000276:immunology* / Q000378:metabolism*; D051381:Rats; D050825:Synaptosomal-Associated Protein 25 / Q000276:immunology* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C494208:SNAP25 protein, human; D050825:Synaptosomal-Associated Protein 25; D029961:Zebrafish Proteins