[Analysis of monosaccharide compositions in Heterosmilax japonica polysaccharide by precolumn derivation HPLC].
OBJECTIVE
To establish precolumn derivation HPLC method to determine the monosaccharides in Heterosmilax japonica polysaccharide.


METHODS
Heterosmilax japonica sample was hydrolyzed with trifluoroacetic (TFA)and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition of Heterosmilax japonica polysaccharide was carried out by reversed-phase technique on a Kromasil C18 column with a mobile phase composed of 100 mmol/L ammonium acetate buffer (pH5.5) and acetonitrile in the ratio of 77: 23. The detection was carried out at 245 nm.


RESULTS
The Heterosmilax japonica polysaccharide composed of mannose, rhamnose, glucose, galactose and xylose with amolar ratio of 66: 1:7590: 452: 528.


CONCLUSIONS
The HPLC method with precolumn derivatization is appropriate for the analysis of monosaccharide composition of Heterosmilax japonica polysaccharide and the method is simple, quick and accurate.
Han
Jing
J
Shenyang Pharmaceutical University, Shenyang 110016, China. hanjing0591@yahoo.cn
Chen
Xiao-Hui
XH
Sun
Li-Xin
LX
Zhan
Zhi-Hui
ZH
Bi
Kai-Shun
KS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D019668:Liliaceae / Q000737:chemistry*; D008358:Mannose / Q000032:analysis; D009005:Monosaccharides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012210:Rhamnose / Q000032:analysis; D027343:Rhizome / Q000737:chemistry
D009005:Monosaccharides; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D012210:Rhamnose; D005690:Galactose