Identification of polymorphisms in the RNase3 gene and the association with allergic rhinitis.
Eosinophil cationic protein (ECP), a potent cytotoxic molecule, is released by activated eosinophils. ECP has been suggested to be involved in tissue remodeling of allergic diseases. The ECP (RNase3) gene is a candidate gene in atopic diseases. RNase3 polymorphisms have been reported to have an association with atopy. We determined whether polymorphisms in the RNase3 gene are associated with allergic rhinitis in a Korean population. The Taqman assay, restriction fragment length polymorphism (PCR-RFLP), and high-resolution melt (HRM) were used for genotyping. Three single nucleotide polymorphisms (SNPs; g.-550A>G, g.371G>C, and g.499G>C) were identified. The genotype of the SNPs was analyzed in patients with allergic rhinitis and controls without allergic rhinitis. The genotype and allele frequencies were compared between both groups. The genotype frequencies of the g.-550A>G and g.371G>C SNPs were not significantly different between patients with allergic rhinitis and controls (P > 0.05). However, in patients with allergic rhinitis, the genotype and allele frequencies of the g.499G>C SNP of RNase 3 were significantly different from those of the control group (P < 001, P = 0.034, respectively). Haplotype analysis demonstrated the presence of the following five different (-550)-(+371)-(+499) major haplotypes: A-G-G, G-C-C, G-G-G, G-C-G, and A-G-C. The G-C-G haplotype was positively associated with allergic rhinitis (P = 0.048), while the G-G-G haplotype was negatively associated with allergic rhinitis (P = 0.004). Our study suggests that RNase3 polymorphisms are potentially associated with susceptibility to allergic rhinitis.
Kang
Inhong
I
Department of Pathology, Wonkwang Institute of Medical Science, Wonkwang University School of Medicine, Iksan, Cheonbuk, South Korea.
An
Xue-hua
XH
Oh
Yeon-Kyun
YK
Lee
Sang Heon
SH
Jung
Ha Min
HM
Chae
Soo-Cheon
SC
Lee
Jae Hoon
JH
D000328:Adult; D000483:Alleles; D047150:Eosinophil Cationic Protein / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D056910:Republic of Korea; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics*; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics*
D047150:Eosinophil Cationic Protein; C479220:RNASE3 protein, human