A versatile approach for assaying in vitro metallodrug metabolism using CE hyphenated with ICP-MS.
An informative analytical method, based on commercially available capillary electrophoresis (CE), inductively coupled plasma mass spectrometry (ICP-MS) and interface units, was developed for ascertaining possible metabolic transformations of metal-based drugs. Using a novel anticancer gallium compound, it was demonstrated that the drug remains intact in the simulated intestine juice. On the contrary, it undergoes a marked change in speciation (mostly, due to binding to transferrin) in human serum.
Abramski
Jan K
JK
Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
Foteeva
Lidia S
LS
Pawlak
Kasia
K
Timerbaev
Andrei R
AR
Jarosz
Maciej
M
D000284:Administration, Oral; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005708:Gallium / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism
D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D014168:Transferrin; D005708:Gallium