title: Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe.
abstract: A hybridization-sensitive fluorescent probe has been designed for nucleic acid detection, using the concept of fluorescence quenching caused by the intramolecular excitonic interaction of fluorescence dyes. We synthesized a doubly thiazole orange-labeled nucleotide showing high fluorescence intensity for a hybrid with the target nucleic acid and effective quenching for the single-stranded state. This exciton-controlled fluorescent probe was applied to living HeLa cells using microinjection to visualize intracellular mRNA localization. Immediately after injection of the probe into the cell, fluorescence was observed from the probe hybridizing with the target RNA. This fluorescence rapidly decreased upon addition of a competitor DNA. Multicoloring of this probe resulted in the simple simultaneous detection of plural target nucleic acid sequences. This probe realized a large, rapid, reversible change in fluorescence intensity in sensitive response to the amount of target nucleic acid, and facilitated spatiotemporal monitoring of the behavior of intracellular RNA.
authors.affiliation: RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan. aki-okamoto@riken.jp
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
authors.forename: Shuji
authors.initials: S
authors.lastname: Ikeda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kubota
authors.forename: Mizue
authors.initials: M
authors.lastname: Yuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yanagisawa
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D013050:Spectrometry, Fluorescence
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D012333:RNA, Messenger; C057718:6-(Dimethylamino)-2-((1E,3E)-4-(4-(dimethylamino)phenyl)-1,3-butadien-1-yl)-1-ethylquinolinium perchlorate