title: Metabolism of stanozolol: chemical synthesis and identification of a major canine urinary metabolite by liquid chromatography-electrospray ionisation ion trap mass spectrometry.
abstract: The canine phase I and phase II metabolism of the synthetic anabolic-androgenic steroid stanozolol was investigated following intramuscular injection into a male greyhound. The major phase I biotransformation was hydroxylation to give 6alpha-hydroxystanozolol which was excreted as a glucuronide conjugate and was identified by comparison with synthetically derived reference materials. An analytical procedure was developed for the detection of this stanozolol metabolite in canine urine using solid phase extraction, enzyme hydrolysis of glucuronide conjugates and analysis by positive ion electrospray ionisation ion trap LC-MS.
authors.affiliation: Research School of Chemistry, Australian National University, Canberra, ACT, Australia.
authors.forename: Rhiannon T
authors.initials: RT
authors.lastname: Stewart
authors.forename: Andrew R
authors.initials: AR
authors.lastname: McKinney
authors.forename: Carmel M
authors.initials: CM
authors.lastname: Kerwick
authors.forename: E Bruce
authors.initials: EB
authors.lastname: Young
authors.forename: Andrew
authors.initials: A
authors.lastname: Vadasz
authors.forename: Ian A
authors.initials: IA
authors.lastname: Cade
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Willis
authors.forename: Malcolm D
authors.initials: MD
authors.lastname: McLeod
mesh_terms: D045930:Anabolic Agents / Q000138:chemical synthesis / Q000493:pharmacokinetics* / Q000652:urine; D000728:Androgens / Q000138:chemical synthesis / Q000493:pharmacokinetics* / Q000652:urine; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D018360:Crystallography, X-Ray; D004285:Dogs; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008958:Models, Molecular; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013197:Stanozolol / Q000138:chemical synthesis / Q000493:pharmacokinetics* / Q000652:urine
chemical_list: D045930:Anabolic Agents; D000728:Androgens; D013197:Stanozolol