Multi-locus variable number tandem repeat analysis for Escherichia coli causing extraintestinal infections.
Discriminatory genotyping methods for the analysis of Escherichia coli other than O157:H7 are necessary for public health-related activities. A new multi-locus variable number tandem repeat analysis protocol is presented; this method achieves an index of discrimination of 99.5% and is reproducible and valid when tested on a collection of 836 diverse E. coli.
Manges
Amee R
AR
Department of Epidemiology, Biostatistics and Occupational Health, McGill University, 1020 Pine Avenue West, Montréal, Québec, Canada.
Tellis
Patricia A
PA
Vincent
Caroline
C
Lifeso
Kimberley
K
Geneau
Geneviève
G
Reid-Smith
Richard J
RJ
Boerlin
Patrick
P
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D008460:Meat / Q000382:microbiology*; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial