Osteoconductive conjugation of bone morphogenetic protein-2 onto titanium/titanium oxide surfaces coated with non-biofouling poly(poly(ethylene glycol) methacrylate).
This paper describes a method for introducing osteoconductivity onto titanium, a widely used material for implants, as well as maintaining its non-biofouling ("bioinert") property, in the aim of increasing bioactivity of titanium for its wider applications to biomedical areas. Titanium substrates were coated with a non-biofouling poly(poly(ethylene glycol) methacrylate) (pPEGMA) by surface-initiated polymerization, and bone morphogenetic protein-2 (BMP-2) was chemically conjugated to the activated pPEGMA films. The non-biofouling property and increased bioactivity of titanium were confirmed by the maintenance of the cellular response of mesenchymal stem cells on the titanium substrates: the BMP-2-conjugated pPEGMA films induced the adhesion and differentiation of mesenchymal stem cells, while non-conjugated pPEGMA films showed the excellent resistance against the adhesion of the cells.
Kang
Sung Min
SM
Department of Chemistry, KAIST, Daejeon 305-701, Republic of Korea.
Kong
Bokyung
B
Oh
Eugene
E
Choi
Joon Sig
JS
Choi
Insung S
IS
D057219:Biofouling / Q000517:prevention & control*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011109:Polymethacrylic Acids; D013055:Spectrophotometry, Infrared; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D011109:Polymethacrylic Acids; C528061:poly(poly(ethylene glycol)methacrylate); C009495:titanium dioxide; D011092:Polyethylene Glycols; D014025:Titanium