title: Prostaglandin synthesis genes are differentially transcripted in normal and pyometra endometria of bitches.
abstract: Pro-inflammatory stimuli, such as endotoxins released by Gram-negative bacteria, are potent stimulators of prostaglandin (PG) synthesis. The aim of this study was to evaluate the gene transcription pattern of PG synthesis enzymes in normal (anestrous, n = 6 and diestrous, n = 8) and pyometra (n = 7) endometria of bitches. Uteri were collected during routine ovariohysterectomy, processed for histopathological evaluation and uterine contents cultured. Gene transcription of COX-1, COX-2, mPGES-1 and PGF-synthase (PGFS) were evaluated by relative real-time PCR and normalized with the ribosomal protein L27 (RPL27) housekeeping gene. Normal uteri had no histological abnormalities and were negative for bacteriology. All pyometra uteri were hyperplasic and Escherichia coli was the only isolated bacterium. Except for COX-1, gene transcription was significantly higher in pyometra than in normal endometria. No significant differences in gene transcription were observed between normal diestrous and anestrous endometria. COX-2 gene transcription was 19 and 69 times higher in pyometra than in diestrous and anestrous endometria (p < 0.001), while PGFS gene transcription had a 3- and 600-fold increase in pyometra endometria compared to normal diestrous and anestrous endometria (p < 0.001). Gene transcription of mPGES-1 was 9 times higher in pyometra than in normal uteri (p < 0.01). Based on these results, we suggest that pyometra-associated E. coli endotoxin release stimulates the up-regulation of COX-2 PGFS and mPGES-1 gene transcription in the endometrium.
authors.affiliation: CIISA, Faculty of Veterinary Medicine, TULisbon, Lisbon, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Silva
authors.forename: S
authors.initials: S
authors.lastname: Leitão
authors.forename: G
authors.initials: G
authors.lastname: Ferreira-Dias
authors.forename: L
authors.initials: L
authors.lastname: Lopes da Costa
authors.forename: L
authors.initials: L
authors.lastname: Mateus
mesh_terms: D000818:Animals; D004285:Dogs; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D011453:Prostaglandins / Q000096:biosynthesis* / Q000235:genetics*; D055112:Pyometra / Q000378:metabolism / Q000662:veterinary*; D014158:Transcription, Genetic
chemical_list: D011453:Prostaglandins