title: Automated assays for sVEGF R1 and PlGF as an aid in the diagnosis of preterm preeclampsia: a prospective clinical study.
abstract: OBJECTIVE
  The purpose of this study was to assess the utility of soluble vascular endothelial growth factor 1 (sVEGF R1) and placental growth factor (PlGF) levels in the clinical diagnosis of preeclampsia.
  
  
  METHODS
  Plasma was collected prospectively from 457 subjects (n = 409 without preeclampsia, n = 48 with preeclampsia) at 20-36 weeks' gestation. Automated immunoassays were used to measure free sVEGF R1 and free PlGF.
  
  
  RESULTS
  Clinical sensitivities of 0.96 and specificities of 0.96 and 0.95 were calculated for sVEGF R1 and PlGF, respectively, for aiding in the diagnosis of preeclampsia. Among subjects with chronic hypertension, sVEGFR1 was dramatically elevated and PlGF decreased in those with superimposed preeclampsia (P < .001 for superimposed preeclampsia vs chronic hypertension for both biomarkers). The ratio of sVEGFR1/PlGF provided a better test to aid in the diagnosis of preeclampsia than either analyte alone (3% false positive rate).
  
  
  CONCLUSIONS
  Free sVEGF R1 and PlGF were useful in differentiating women with preterm preeclampsia from normotensive and hypertensive subjects.
authors.affiliation: Department of Maternal-Fetal Medicine, The Toledo Hospital, OH 43606, USA. shiraz.sunderjimd@promedica.org
authors.forename: Shiraz
authors.initials: S
authors.lastname: Sunderji
authors.forename: Emanuel
authors.initials: E
authors.lastname: Gaziano
authors.forename: Donald
authors.initials: D
authors.lastname: Wothe
authors.forename: Linda C
authors.initials: LC
authors.lastname: Rogers
authors.forename: Baha
authors.initials: B
authors.lastname: Sibai
authors.forename: S Ananth
authors.initials: SA
authors.lastname: Karumanchi
authors.forename: Cheryl
authors.initials: C
authors.lastname: Hodges-Savola
mesh_terms: D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000175:diagnosis*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D011446:Prospective Studies; D012372:ROC Curve; D012680:Sensitivity and Specificity; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*
chemical_list: C000608036:PGF protein, human; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1