title: DOSY NMR applied to analysis of flavonoid glycosides from Bidens sulphurea.
abstract: 2D DOSY 1H NMR has proved to be a useful technique in the identification of the molecular skeleton of the four major compounds of ethyl acetate extract of aerial parts of Bidens sulphurea (Asteraceae). The combination of this technique with HPLC, mass spectrometry and other NMR techniques enabled the identification of four flavonoid glycosides: quercetin-3-O-beta-D-galactopyranoside, quercetin-3-O-beta-D-glycopyranoside, quercetin-3-O-alpha-L-arabinofuranoside and quercetin-3-O-beta-D-rhamnopyranoside.
authors.affiliation: Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida dos Bandeirantes 3900, 14040-901 Ribeirão Preto, SP, Brazil. delphino@pg.ffclrp.usp.br
authors.forename: Edilene Delphino
authors.initials: ED
authors.lastname: Rodrigues
authors.forename: Denise Brentan
authors.initials: DB
authors.lastname: da Silva
authors.forename: Dionéia Camilo Rodrigues
authors.initials: DC
authors.lastname: de Oliveira
authors.forename: Gil Valdo José
authors.initials: GV
authors.lastname: da Silva
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D012015:Reference Standards
chemical_list: D005419:Flavonoids; D006027:Glycosides