Pseudomonas fluorescens CHA0 can kill subterranean termite Odontotermes obesus by inhibiting cytochrome c oxidase of the termite respiratory chain.
Pseudomonas fluorescens CHA0 has been shown to suppress the growth of a wide range of microbial plant pathogens as well as invertebrate pests such as root nematodes. Hydrogen cyanide, a secondary metabolite produced by the bacterium, has been credited as being one of the determinants of its biocontrol ability. The use of biocontrol agents against social insect pests such as termite Odontotermes obesus has limitations because of behavioural adaptations that include (1) removal of the pathogen when grooming by the termites and (2) isolation of infested members of the colony. In this study, we show that cyanide of bacterial origin may inhibit cytochrome c oxidase (CCO) of the termite respiratory chain and demonstrate that HCN-producing bacteria such as P. fluorescens can actually kill a macroscopic insect pest by cyanide poisoning. This ability of pseudomonad metabolites such as cyanide, which can bring about pest death by blocking respiration through inhibition of CCO rather than infection or predation, can potentially overcome the behavioural adaptations of social insect pests such as termites and represents an attractive option for insect pest management.
Devi
Karunanidhi Kanchana
KK
Laboratory of Soil Biology and Microbial Ecology, Centre for Environmental Management of Degraded Ecosystems, University of Delhi, Delhi, India. dmkothamasi@cemde.du.ac.in
Kothamasi
David
D
D000818:Animals; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D006856:Hydrogen Cyanide / Q000378:metabolism* / Q000633:toxicity*; D020049:Isoptera / Q000382:microbiology*; D011552:Pseudomonas Infections / Q000382:microbiology* / Q000662:veterinary; D011551:Pseudomonas fluorescens / Q000472:pathogenicity*; D016019:Survival Analysis
D006856:Hydrogen Cyanide; D003576:Electron Transport Complex IV