L-ribose production from L-arabinose by using purified L-arabinose isomerase and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans.
Two enzymes, L-arabinose isomerase and mannose-6-phosphate isomerase, from Geobacillus thermodenitrificans produced 118 g/liter L-ribose from 500 g/liter L-arabinose at pH 7.0, 70 degrees C, and 1 mM Co(2+) for 3 h, with a conversion yield of 23.6% and a volumetric productivity of 39.3 g liter(-1) h(-1).
Yeom
Soo-Jin
SJ
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
Kim
Nam-Hee
NH
Park
Chang-Su
CS
Oh
Deok-Kun
DK
D019747:Aldose-Ketose Isomerases / Q000302:isolation & purification* / Q000378:metabolism*; D001089:Arabinose / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D020536:Enzyme Activators / Q000494:pharmacology; D056506:Geobacillus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008359:Mannose-6-Phosphate Isomerase / Q000302:isolation & purification* / Q000378:metabolism*; D012266:Ribose / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D020536:Enzyme Activators; D003035:Cobalt; D012266:Ribose; D001089:Arabinose; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase; D008359:Mannose-6-Phosphate Isomerase