[Purification of EGCG monomer from the extracts of tea by high speed countercurrent chromatography].
OBJECTIVE
To investigate the feasibility and techniques for purifying epigallocatechin-3-gallate (EGCG) by high speed countercurrent chromatography (HSCCC).


METHODS
A HSCCC unit was used for the purification of EGCG from the extracts of tea.


RESULTS
The preferable solvent system was composed of Ether-Ethyl Acetate-H2O (4:10: 25, V/V), and the preferable technical conditions were as follows: the temperature was 35 degrees C, the rotation speed was 800 r/min and the mobile phase flow-rate was 2.5 mL/min.


CONCLUSIONS
The purity of the obtained EGCG sample determined by high performance liquid chromatography (HPLC) is 95%.
Zhang
Yang
Y
School of Chemistry & Chemical Engineering, South China University of Technology, Guangzhou 510640, China.
Jiang
Yan-Bin
YB
Huang
Shao-Lie
SL
Zheng
Ming-Ying
MY
D000085:Acetates / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D004986:Ether / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D013662:Tea / Q000737:chemistry*
D000085:Acetates; D010936:Plant Extracts; D012997:Solvents; D013662:Tea; D004986:Ether; D002392:Catechin; C045651:epigallocatechin gallate