Concentration polarization of high-density lipoprotein and its relation with shear stress in an in vitro model.
The purpose of this study was to determine the concentration polarization of high-density lipoprotein (HDL) at the surface of the carotid artery under conditions of steady flow and to establish its relationship with shear stress using an in vitro vascular simulation model of carotid bifurcation. Shear stress, HDL concentration at the surface, and the ratio of HDL concentration at the surface to concentration in bulk flow were measured at different locations within the model under high-speed (1.451 m/s) and low-speed (0.559 m/s) flow. HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus. With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased. The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).
Meng
Wei
W
Department of Thoracic and Cardiovascular Surgery, West China Medical Center, Sichuan University, Chengdu, China.
Yu
Fengxu
F
Chen
Huaiqing
H
Zhang
Jianmin
J
Zhang
Eryong
E
Dian
Ke
K
Shi
Yingkang
Y
D001783:Blood Flow Velocity / Q000502:physiology; D002339:Carotid Arteries / Q000502:physiology*; D003198:Computer Simulation; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry* / Q000502:physiology*; D008955:Models, Cardiovascular; D018709:Statistics, Nonparametric; D013314:Stress, Mechanical
D008075:Lipoproteins, HDL