A molecular and physiological survey of a diverse collection of hydrothermal vent Thermococcus and Pyrococcus isolates.
Strains of hyperthermophilic anaerobic hydrothermal vent archaea maintained in the culture collection assembled by Holger Jannasch at the Woods Hole Oceanographic Institution between 1984 and 1998 were identified and partially characterized by Denaturing Gradient Gel Electrophoresis, 16S rRNA gene sequencing, and by growth tests at different temperatures and on different organic carbon and nitrogen sources. All strains were members of the genera Thermococcus and Pyrococcus. The greatest phylogenetic diversity was found in strains from a single Guaymas Basin core isolated by serial dilution from four different depth horizons of heated sediment incubated at the corresponding in situ temperatures. In contrast, geographically distinct vent locations and sample materials yielded a lower diversity of isolates when enriched under uniform temperature regimes and without prior dilution of the source material.
Teske
Andreas
A
Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. teske@email.unc.edu
Edgcomb
Virginia
V
Rivers
Adam R
AR
Thompson
Janelle R
JR
de Vera Gomez
Alvin
A
Molyneaux
Stephen J
SJ
Wirsen
Carl O
CO
D001431:Bacteriological Techniques; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D045482:Hot Springs / Q000382:microbiology*; D008386:Marine Biology; D008800:Mexico; D008969:Molecular Sequence Data; D010802:Phylogeny; D019711:Pyrococcus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D013045:Species Specificity; D013696:Temperature; D019712:Thermococcus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S