Protective effect of Antrodia camphorata on bladder ischemia/reperfusion injury.
BACKGROUND
Obstructive bladder dysfunction is directly related to ischemia/reperfusion injury characterized by damage to nerves, synapses and smooth muscle cells within the bladder wall. Antrodia Camphorata (AC) has significant antioxidant, antiinflammatory and cell-cycle inhibition properties. The specific aim of this study was to evaluate whether orally administered AC can protect rabbit bladders from the progressive dysfunctions induced by bilateral ischemia/reperfusion (I/R).


METHODS
Twenty-four male NZW rabbits were separated into 4 groups of 6 animals each. Rabbits in groups 1 and 2 were fed Antrodia Camphorata (AC) suspensions; those in groups 3 and 4 received vehicle. Each rabbit in groups 2 and 4 were subjected to in vivo bilateral ischemia for 2 h and then allowed to recover for 1 week. The rabbits in groups 1 and 3 received sham operation and served as control groups. Cystometry, contractile responses to field stimulation, carbachol, ATP and KCl were determined. Biochemical and immuno-histochemical studies were also performed.


RESULTS
I/R resulted in decreased compliance, decreased contractile responses, decreased nerve density, and increased apoptosis. AC pretreatment of rabbits subjected to I/R significantly protected the bladder from all contractile, biochemical, and structural dysfunctions resulting in significantly improved bladder.
Juan
Yung-Shun
YS
Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan.
Mannikarottu
Anita
A
Chuang
Shu Mien
SM
Li
Suning
S
Lin
Alpha D
AD
Chang-Chou
Lan
L
Schuler
Catherine
C
Leggett
Robert E
RE
Levin
Robert M
RM
D000284:Administration, Oral; D000818:Animals; D055491:Antrodia; D017209:Apoptosis; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000473:pathology / Q000503:physiopathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage*; D011817:Rabbits; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D001743:Urinary Bladder / Q000473:pathology / Q000503:physiopathology*
D010936:Plant Extracts