Detoxification of mercury species--an in vitro study with antidotes in human whole blood.
To investigate the effects of mercury species intoxication and to test the efficiency of different commonly applied antidotes, human whole blood and plasma surrogate samples were spiked with inorganic mercury (Hg2+) and methylmercury (MeHg+, CH3Hg+) prior to treatment with the antidotes 2,3-dimercaptopropan-1-ol (British Anti Lewisite), 2,3-dimercaptosuccinic acid (DMSA), and N-acetylcysteine (NAC). For mercury speciation analysis in these samples, liquid chromatography was coupled to either inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionisation time-of-flight mass spectrometry (ESI-TOF-MS). Adduct formation between mercury species and physiological thiols (cysteine and glutathione) was observed as well as the release of glutathione under treatment with the antidotes DMSA and NAC.
Trümpler
Stefan
S
University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 30, 48149 Münster, Germany.
Nowak
Sascha
S
Meermann
Björn
B
Wiesmüller
Gerhard A
GA
Buscher
Wolfgang
W
Sperling
Michael
M
Karst
Uwe
U
D000328:Adult; D000931:Antidotes / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008658:Inactivation, Metabolic; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008628:Mercury / Q000097:blood / Q000493:pharmacokinetics*; D008767:Methylmercury Compounds / Q000097:blood / Q000493:pharmacokinetics*; D008954:Models, Biological
D000931:Antidotes; D008767:Methylmercury Compounds; D008628:Mercury