High expression of antizyme inhibitor 2, an activator of ornithine decarboxylase in steroidogenic cells of human gonads.
High activity of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine synthesis, is typically present in rapidly proliferating normal and malignant cells. The mitotically inactive steroidogenic cells in rodent testis and ovaries, however, also display high ODC activity. The activity of ODC in these cells responds to luteinizing hormone, and inhibition of ODC reduces the production of steroid hormones. Polyamines and ODC also control proliferation of germ cells and spermiogenesis. The activity of ODC, especially in proliferating cells, is regulated by antizyme inhibitor (AZIN). This protein displaces ODC from a complex with its inhibitor, antizyme. We have previously identified and cloned a second AZIN, i.e. antizyme inhibitor 2 (AZIN2), which has the highest levels of expression in brain and in testis. In the present study, we have used immunohistochemistry and in situ hybridization to localize the expression of AZIN2 in human gonads. We found a robust expression of AZIN2 in steroidogenic cells: testicular Leydig cells and Leydig cell tumors, in ovarian luteinized cells lining corpus luteum cysts, and in hilus cells. The results suggest that AZIN2 is not primarily involved in regulating the proliferation of the germinal epithelium, indicating a different role for AZIN1 and AZIN2 in the regulation of ODC. The localization of AZIN2 implies possible involvement in the gonadal synthesis and/or release of steroid hormones.
Mäkitie
Laura T
LT
Department of Pathology, Haartman Institute, University of Helsinki, P.O. Box 21, 00014, Helsinki, Finland.
Kanerva
Kristiina
K
Sankila
Anna
A
Andersson
Leif C
LC
D002262:Carboxy-Lyases; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D005260:Female; D006066:Gonads / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007984:Leydig Cell Tumor / Q000737:chemistry; D007985:Leydig Cells / Q000737:chemistry; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010048:Ovarian Cysts / Q000737:chemistry; D013256:Steroids / Q000096:biosynthesis*
D002352:Carrier Proteins; D013256:Steroids; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; C546395:AZIN2 protein, human