Titanium(IV) complexes: cytotoxicity and cellular uptake of titanium(IV) complexes on caco-2 cell line.
Replacement of the ancillary ligand in titanocene dichloride by amino acids provides titanocene species with high water solubility. As part of our research efforts in the area of titanium-based antitumor agents, we have investigated the cytotoxic activity of Cp(2)TiCl(2) and three water soluble titanocene-amino acid complexes - [Cp(2)Ti(aa)(2)]Cl(2) (aa=L-cysteine, L-methionine, and D-penicillamine) and one water soluble coordination compound, [Ti(4)(maltolato)(8)(micro-O)(4)] on the human colon adenocarcinoma cell line, Caco-2. At pH of 7.4 all titanocene species decompose extensively while [Ti(4)(maltolato)(8)(micro-O)(4)] is stable for over seven days. In terms of cytotoxicity, the [Cp(2)Ti(aa)(2)]Cl(2) and [Ti(4)(maltolato)(8)(micro-O)(4)] complexes exhibited slightly higher toxicity than titanocene dichloride at 24h, but at 72h titanocene dichloride and [Ti(4)(maltolato)(8)(micro-O)(4)] have higher cytotoxic activity. Cellular titanium uptake was quantified at various time intervals to investigate the possible relationship between Ti uptake and cellular toxicity. Results indicated that there was not a clear relationship between Ti uptake and cytotoxicity. A structure-activity relationship is discussed.
Hernández
Ramón
R
University of Puerto Rico, Department of Chemistry, Mayaguez,, Puerto Rico.
Méndez
Janet
J
Lamboy
José
J
Torres
Madeline
M
Román
Féliz R
FR
Meléndez
Enrique
E
D000970:Antineoplastic Agents / Q000378:metabolism / Q000633:toxicity*; D001667:Binding, Competitive / Q000187:drug effects; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004996:Ethidium / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000378:metabolism / Q000633:toxicity*; D013054:Spectrophotometry, Atomic; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000378:metabolism / Q000633:toxicity*
D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D004247:DNA; D014025:Titanium; D004996:Ethidium; C020528:titanocene dichloride