title: Transforming growth factorbeta(1) transactivates EGFR via an H(2)O(2)-dependent mechanism in squamous carcinoma cell line.
abstract: TGFbeta is known to transactivate EGFR. However, the signaling component involved in this crosstalk has yet to be revealed. Here, we found that TGFbeta(1) phosphorylated EGFR in a dose-dependent manner in SCC13 and A431 cells, and it was not blocked by EGF-neutralizing antibody. H(2)O(2) was increased by TGFbeta(1) treatment in the same time-kinetics as EGFR activation. Pretreatment of N-acetyl cysteine abolished TGFbeta(1)-induced H(2)O(2) induction and EGFR activation. Direct treatment of H(2)O(2) phosphorylated EGFR and catalase inhibitor prolonged TGFbeta(1)-induced EGFR activation. These results show that TGFbeta(1) activates EGFR via an H(2)O(2)-dependent mechanism, which subsequently leads to the activation of Erk(1/2).
authors.affiliation: Department of Genetic Engineering, Musculoskeletal Bioorgan Center, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: EunAh
authors.initials: E
authors.lastname: Lee
authors.forename: Jae Youn
authors.initials: JY
authors.lastname: Yi
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Chung
authors.forename: Youngsook
authors.initials: Y
authors.lastname: Son
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015151:Immunoblotting; D047468:Immunoprecipitation; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D020239:Receptor Cross-Talk; D015398:Signal Transduction / Q000502:physiology*; D015533:Transcriptional Activation; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D053773:Transforming Growth Factor beta1; D006861:Hydrogen Peroxide; D066246:ErbB Receptors; D048049:Extracellular Signal-Regulated MAP Kinases