Assessment of the DNA damage using the fluorescence microscope.
DNA damage was monitored by fluorescent microscopy observations of DNA fluorescent images after hydrodynamic stretching on a microscope glass. DNA double-strand breaks lead to a decrease of the average length of observed fluorescent DNA molecules. Compared to conventional methods such as electrophoresis, the proposed method allows for the analysis of the DNA damage at very low DNA breaking frequency. In particular, this method was used to study DNA damage by weak UV irradiation in solutions of quantum dots.
Yamazaki
Yuka
Y
Graduate School of Environmental Studies, Nagoya University, Nagoya, Aichi 464-8601, Japan.
Zinchenko
Anatoly
A
Murata
Shizuaki
S
D004247:DNA / Q000528:radiation effects / Q000648:ultrastructure; D004249:DNA Damage; D008856:Microscopy, Fluorescence; D045663:Quantum Dots; D014466:Ultraviolet Rays
D004247:DNA