Flow cytometric analysis of benthic prokaryotes attached to sediment particles.
Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity.
Amalfitano
Stefano
S
Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, Monterotondo, Rome, Italy. amalfitano@irsa.cnr.it
Puddu
Stefano Fazi Alberto
SF
D005434:Flow Cytometry / Q000379:methods*; D019015:Geologic Sediments / Q000382:microbiology*; D009696:Nucleic Acids / Q000032:analysis / Q000235:genetics; D011387:Prokaryotic Cells / Q000737:chemistry / Q000145:classification*; D045483:Rivers; D013194:Staining and Labeling / Q000379:methods
D009696:Nucleic Acids