Antibodies against recombinant heat shock proteins of 60 kDa from enterobacteria in the sera and synovial fluid of HLA-B27 positive ankylosing spondylitis patients.
OBJECTIVE
To study the association of HLA-B27 with IgG antibodies to different enterobacterial HSP60s in patients with ankylosing spondylitis (AS).


METHODS
IgG antibodies to 60 kDa enterobacterial HSPs were determined by ELISA in paired samples of sera and synovial fluid from 21 HLA-B27+ ankylosing spondylitis (AS) patients; and in sera from 32 HLA-B27+ AS patients, 35 HLA-B27+ healthy relatives of AS patients, and 60 HLA-B27- healthy individuals with no family members with AS.


RESULTS
HLA-B27+ patients and healthy individuals showed significantly higher IgG antibody levels to recombinant enterobacterial HSP60s than HLA-B27- healthy controls. The levels of anti-HSP60Sf and anti-HSP60Ec antibodies correlated with disease activity and anti-HSP60Ec antibodies with male gender. No association between enterobacterial HSP60 antibody levels and disease duration was observed. All groups had lower levels of IgG antibodies to rHSP60 from Streptococcus pyogenes (rHSP60 Spy). In paired samples of sera and synovial fluid from B27+ patients, IgG antibodies to enterobacterial HSP60s were detected, but in significantly higher levels in sera than in synovial fluid. The anti-rHSPSpy IgG response in these samples was lower and similar in the three groups.


CONCLUSIONS
A correlation was found between HLA-B27 and the response to recombinat enterobacterial HSP60s. This response could be associated with disease activitir and gender in some proteins and the presence eof IgG antibodies to these proteins in synovial fluid could be associated with the inflammatory process and initiation of AS.
Domínguez-López
M L
ML
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico. ldmguez@yahoo.com.mx
Ortega-Ortega
Y
Y
Manríquez-Raya
J C
JC
Burgos-Vargas
R
R
Vega-López
A
A
García-Latorre
E
E
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000097:blood / Q000150:complications / Q000276:immunology*; D005260:Female; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D008297:Male; D008800:Mexico; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D013167:Spondylitis, Ankylosing / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D013582:Synovial Fluid / Q000276:immunology* / Q000382:microbiology; D055815:Young Adult
D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D015796:HLA-B27 Antigen; D011994:Recombinant Proteins