Correlative fluorescence and electron microscopy in tissues: immunocytochemistry.
Correlative microscopy is a collection of procedures that rely upon two or more imaging modalities to examine the same specimen. The imaging modalities employed should each provide unique information and the combined correlative data should be more information rich than that obtained by any of the imaging methods alone. Currently the most common form of correlative microscopy combines fluorescence and electron microscopy. While much of the correlative microscopy in the literature is derived from studies of model cell culture systems we have focused, primarily, on correlative microscopy in tissue samples. The use of tissue, particularly human tissue, may add constraints not encountered in cell culture systems. Ultrathin cryosections, typically used for immunoelectron microscopy, have served as the substrate for correlative fluorescence and electron microscopic immunolocalization in our studies. In this work, we have employed the bifunctional reporter FluoroNanogold. This labeling reagent contains both a fluorochrome and a gold-cluster compound and can be imaged by sequential fluorescence and electron microscopy. This approach permits the examination of exactly the same sub-cellular structures in both fluorescence and electron microscopy with a high level of spatial resolution.
Robinson
J M
JM
Department of Physiology and Cell Biology, Ohio State University, Columbus, Ohio, USA. robinson.21@osu.edu
Takizawa
T
T
D002824:Chorionic Villi / Q000737:chemistry / Q000648:ultrastructure; D005453:Fluorescence; D006046:Gold; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D053758:Nanoparticles; D009504:Neutrophils / Q000737:chemistry / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods*
D006046:Gold