Ferredoxin:NADP+ oxidoreductase association with phycocyanin modulates its properties.
In photosynthetic organisms, ferredoxin:NADP(+) oxidoreductase (FNR) is known to provide NADPH for CO(2) assimilation, but it also utilizes NADPH to provide reduced ferredoxin. The cyanobacterium Synechocystis sp. strain PCC6803 produces two FNR isoforms, a small one (FNR(S)) similar to the one found in plant plastids and a large one (FNR(L)) that is associated with the phycobilisome, a light-harvesting complex. Here we show that a mutant lacking FNR(L) exhibits a higher NADP(+)/NADPH ratio. We also purified to homogeneity a phycobilisome subcomplex comprising FNR(L,) named FNR(L)-PC. The enzymatic activities of FNR(L)-PC were compared with those of FNR(S). During NADPH oxidation, FNR(L)-PC exhibits a 30% decrease in the Michaelis constant K(m)((NADPH)), and a 70% increase in K(m)((ferredoxin)), which is in agreement with its predicted lower activity of ferredoxin reduction. During NADP(+) reduction, the FNR(L)-PC shows a 29/43% decrease in the rate of single electron transfer from reduced ferredoxin in the presence/absence of NADP(+). The increase in K(m)((ferredoxin)) and the rate decrease of single reduction are attributed to steric hindrance by the phycocyanin moiety of FNR(L)-PC. Both isoforms are capable of catalyzing the NADP(+) reduction under multiple turnover conditions. Furthermore, we obtained evidence that, under high ionic strength conditions, electron transfer from reduced ferredoxin is rate limiting during this process. The differences that we observe might not fully explain the in vivo properties of the Synechocystis mutants expressing only one of the isoforms. Therefore, we advocate that FNR localization and/or substrates availability are essential in vivo.
Korn
Anja
A
Institut de Biologie et de Technologie de Saclay, Commissariat à L'Energie Atomique, CNRS, F-91191 Gif sur Yvette, France.
Ajlani
Ghada
G
Lagoutte
Bernard
B
Gall
Andrew
A
Sétif
Pierre
P
D002457:Cell Extracts; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D009249:NADP / Q000378:metabolism*; D009994:Osmolar Concentration; D045524:Phycobilisomes / Q000201:enzymology* / Q000235:genetics; D010798:Phycocyanin / Q000378:metabolism*; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics
D002457:Cell Extracts; D045524:Phycobilisomes; D010798:Phycocyanin; D009249:NADP; D005287:Ferredoxin-NADP Reductase