[Simultaneous determination of anthraquinones in Liuwei'anxiao capsules by HPLC].
OBJECTIVE
To establish an RP-HPLC method for measuring the contents of aloe-emodin, rhein, emodin, chrysophanol and physion in Liuwei'anxiao Capsules (traditional Chinese medicine).


METHODS
The analysis was carried out with a Diamonsil-C18 column (200 mm x 4.6 mm, 5 microm) and a mobile phase of methanol-0.05% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL/min and the column temperature was 35 degrees C. The detection wavelength was 254 nm.


RESULTS
The calibration curve was linear within the range of 1.622 - 16.22 microg/mL (r1 = 0.9998), 0.6960 - 6.960 microg/mL (r2 = 0.9998), 2.448 - 24.48 microg/mL (r3 = 0.9995), 2.752 - 27.52 microg/mL (r4 = 0.9997), 2.702 -27.02 microg/mL (r = 0.9997) for aloe-emodin, rhein, emodin, chrysophanol and physion, respectively.


CONCLUSIONS
This method is convenient, accurate and reproducible for the quality control of Liuwei'anxiao Capsules.
Jiang
Bo
B
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. rabbitbo@sina.com
Gao
Xun
X
Bi
Kai-Shun
KS
Chen
Xiao-Hui
XH
D000880:Anthraquinones / Q000032:analysis*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D004642:Emodin / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D000880:Anthraquinones; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D004642:Emodin; C027113:chrysophanic acid; C020491:rhein