Protective effects of aqueous extract of Artemisia campestris against puffer fish Lagocephalus lagocephalus extract-induced oxidative damage in rats.
The aerial parts of Artemisia campestris are often used in Tunisian poisoning cases and are known to possess significant antioxidant activities. The objective of this study is to evaluate the protective effects of an aqueous extract (5g/l) of A. campestris leaves and stems (AE), on oxidative damages induced by liver extract (LT) from poisonous fish Lagocephalus lagocephalus in Wistar rats. AE was found to contain large amounts of K(+), Na(+), Ca(++) and significant antioxidant capacities highlighted by high level of polyphenols and scavenging activities for DPPH and superoxide anion. LT-injected rats (1ml/100g body wt) for 10 days showed (1) a reduced appetite and diarrhea resulting in a lower growth rate than controls, (2) a decrease in serum ALT and AST activities suggesting liver functional disorders, (3) an increase of serum urea and creatinine and reduced serum sodium and potassium concentrations highlighting renal insufficiency and (4) an oxidative stress as evidenced by the raise of TBARS and the inhibition of SOD, CAT and GSH-Px activities in liver, kidney and brain tissues Absorption of AE as a drink, for 20 days (10 pre-treatment days+10 experiment days) did not lead significant change of studied parameters but prevented all the disorders induced by LT.
Saoudi
Mongi
M
Animal Ecophysiology Laboratory, Marine Biotechnology and Biodiversity Laboratory, Department of Life Sciences, Sciences Faculty of Sfax, Sfax, Tunisia.
Allagui
Mohamed Salah
MS
Abdelmouleh
Abdelwaheb
A
Jamoussi
Kamel
K
El Feki
Abdelfattah
A
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008903:Minerals / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D023721:Tetraodontiformes; D014020:Tissue Extracts / Q000633:toxicity
D000975:Antioxidants; D008903:Minerals; D010936:Plant Extracts; D014020:Tissue Extracts; D002374:Catalase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase