Effects of blood contamination on peritoneal D-dimer concentration in horses with colic.
BACKGROUND
Peritoneal D-Dimer concentration can be determined to assess peritoneal fibrinolysis activity in horses with gastrointestinal disorders. However, blood contamination of peritoneal fluid may occur during collection and could alter peritoneal D-Dimer concentration.


OBJECTIVE
Blood contamination in peritoneal fluid does not affect interpretation of peritoneal D-Dimer concentration in horses with colic.


METHODS
Thirty-four horses with colic and 4 healthy horses.


METHODS
Peritoneal fluid and blood samples were simultaneously collected upon admission. Then, peritoneal fluid was serially contaminated with the horse's own blood; final contaminations corresponded to 1, 5, 10, and 20% of blood in peritoneal fluid. D-Dimer concentration was determined in blood, peritoneal fluid, and contaminated peritoneal fluid samples. Data were analyzed using a longitudinal linear model and a generalized estimating equations analysis to assess the quantitative and qualitative variations of the effect of blood contamination on peritoneal D-Dimer concentration.


RESULTS
Peritoneal D-Dimer concentration was only quantitatively affected when peritoneal fluid was contaminated at 20% of blood. However, when using increasing cut-off values of peritoneal D-Dimer concentration (100, 2,000, 8,000, and 16,000 ng/mL), this effect disappeared at the highest cut-off values (8,000 and 16,000 ng/mL). When peritoneal fluid contamination was grouped as "minimally contaminated" (< or =1% of blood) and "highly contaminated" (> or =5% of blood), no significant differences on D-Dimer concentration between both groups at each cut-off value were observed.


CONCLUSIONS
Although quantitative results of peritoneal D-Dimer concentration could be affected by high levels of blood contamination (> or =20%), interpretation of increased peritoneal fibrinolytic activity was not significantly affected.
Delgado
M A
MA
Departament de Medicina i Cirurgia Animals, Servei de Medicina Interna Equina, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain.
Monreal
L
L
Armengou
L
L
Segura
D
D
Ríos
J
J
D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry*; D015415:Biomarkers; D001769:Blood; D003085:Colic / Q000175:diagnosis / Q000662:veterinary*; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006734:Horse Diseases / Q000097:blood / Q000175:diagnosis*; D006736:Horses; D008297:Male; D013048:Specimen Handling / Q000662:veterinary
D015415:Biomarkers; D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D