title: Selection of colistin-resistant Acinetobacter baumannii isolates in postneurosurgical meningitis in an intensive care unit with high presence of heteroresistance to colistin.
abstract: Colistin heteroresistance in Acinetobacter baumannii (Ab) has been reported, but the clinical impact and the antimicrobial treatment have not been established yet. We observed the selection intratreatment with colistin of Ab colistin-resistant strains from a colistin-heteroresistant isolate in one patient with postneurosurgical meningitis. The presence and the genetic relationship of heteroresistant Ab isolates from intensive care units (ICUs) obtained in the same period of the case report were analyzed. Twenty-eight isolates from patients admitted to the ICUs of an Argentinian university hospital during June to December 2004 were evaluated. Genomoespecie was determined by amplified ribosomal DNA restriction analysis, and genetic similarity among the strains was determined by pulsed-field electrophoresis. Colistin heteroresistance was observed in 46, 4% of these isolates. The majority belonged to clones previously identified as I and III.
authors.affiliation: Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 1120 Buenos Aires, Argentina. carlos_hernanrodriguez@hotmail.com.ar
authors.forename: Carlos Hernan
authors.initials: CH
authors.lastname: Rodriguez
authors.forename: Rodriguez Carlos
authors.initials: RC
authors.lastname: Hernan
authors.forename: Karina
authors.initials: K
authors.lastname: Bombicino
authors.forename: Bombicino
authors.initials: B
authors.lastname: Karina
authors.forename: Gabriela
authors.initials: G
authors.lastname: Granados
authors.forename: Granados
authors.initials: G
authors.lastname: Gabriela
authors.forename: Marcela
authors.initials: M
authors.lastname: Nastro
authors.forename: Nastro
authors.initials: N
authors.lastname: Marcela
authors.forename: Carlos
authors.initials: C
authors.lastname: Vay
authors.forename: Vay
authors.initials: V
authors.lastname: Carlos
authors.forename: Angela
authors.initials: A
authors.lastname: Famiglietti
authors.forename: Famiglietti
authors.initials: F
authors.lastname: Angela
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001118:Argentina; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003091:Colistin / Q000494:pharmacology / Q000627:therapeutic use*; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006785:Hospitals, University; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D016920:Meningitis, Bacterial / Q000188:drug therapy / Q000382:microbiology*; D021521:Ribotyping; D012641:Selection, Genetic; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D003091:Colistin