Marine compound Xyloketal B protects PC12 cells against OGD-induced cell damage.
Xyloketal B is a novel marine compound with unique chemical structure isolated from mangrove fungus Xylaria sp. (no. 2508). Recently, we have demonstrated that Xyloketal B is an antioxidant and can protect against oxidized low density lipoprotein (LDL)-induced cell injury. In the present study, we investigated whether Xyloketal B can protect against ischemia-induced cell injury in an in vitro oxygen glucose deprivation (OGD) model of ischemic stroke in PC12 cells. We found that Xyloketal B could directly scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and protect PC12 cells against OGD insult. Furthermore, Xyloketal B alleviated OGD-induced mitochondria superoxide, mitochondria fragmentation and GTPase dynamin-related protein 1 (Drp1) overexpression as well as reduction of mitochondrial membrane potential. All together, the present study demonstrates that Xyloketal B protects PC12 cells against OGD-induced cell injury and that the anti-oxidative property and protective action on mitochondria may account for its neuroprotective actions.
Zhao
Jia
J
Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, PR China.
Li
Ling
L
Ling
Chen
C
Li
Jie
J
Pang
Ji-Yan
JY
Lin
Yong-Cheng
YC
Liu
Jie
J
Huang
Ruxun
R
Wang
Guan-Lei
GL
Pei
Zhong
Z
Zeng
Jinsheng
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use; D005609:Free Radicals / Q000037:antagonists & inhibitors / Q000378:metabolism; D008954:Models, Biological; D009410:Nerve Degeneration / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D011714:Pyrans / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D020521:Stroke / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology
D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D018696:Neuroprotective Agents; D011714:Pyrans; C515343:xyloketal B