title: Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine and 5-methyl-2-thiodeoxycytosine.
abstract: For more effective DNA triplex formation under neutral conditions, we synthesized triplex-forming oligonucleotides (TFO) containing 8-thioxodeoxyadenine (s(8)A) residues in place of the protonated cytosines (Cs) required for the third base pairing with DNA duplexes. Consequently, it was found that s(8)A exhibited much stronger hybridization ability than C under neutral conditions when four s(8)A bases were arranged in a consecutive sequence. Moreover, we also synthesized TFOs containing 5-methyl-2-thiocytosines and examined their ability of triplex formation.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Kenichi
authors.initials: K
authors.lastname: Miyata
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tsunoda
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D003839:Deoxyadenosines / Q000737:chemistry*; D003845:Deoxycytosine Nucleotides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009946:Organothiophosphorus Compounds / Q000737:chemistry*
chemical_list: C535199:8-thioxodeoxyadenosine; D003839:Deoxyadenosines; D003845:Deoxycytosine Nucleotides; D009838:Oligodeoxyribonucleotides; D009946:Organothiophosphorus Compounds; C051802:triplex DNA; D004247:DNA