title: Changes in oil content of transgenic soybeans expressing the yeast SLC1 gene.
abstract: The wild type (Wt) and mutant form of yeast (sphingolipid compensation) genes, SLC1 and SLC1-1, have been shown to have lysophosphatidic acid acyltransferase (LPAT) activities (Nageic et al. in J Biol Chem 269:22156-22163, 1993). Expression of these LPAT genes was reported to increase oil content in transgenic Arabidopsis and Brassica napus. It is of interest to determine if the TAG content increase would also be seen in soybeans. Therefore, the wild type SLC1 was expressed in soybean somatic embryos under the control of seed specific phaseolin promoter. Some transgenic somatic embryos and in both T2 and T3 transgenic seeds showed higher oil contents. Compared to controls, the average increase in triglyceride values went up by 1.5% in transgenic somatic embryos. A maximum of 3.2% increase in seed oil content was observed in a T3 line. Expression of the yeast Wt LPAT gene did not alter the fatty acid composition of the seed oil.
authors.affiliation: Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Suryadevara S
authors.initials: SS
authors.lastname: Rao
authors.forename: David
authors.initials: D
authors.lastname: Hildebrand
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics*; D004398:Dyneins; D030821:Plants, Genetically Modified / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D013024:Soybean Oil / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D013025:Glycine max / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D013024:Soybean Oil; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase; D004398:Dyneins; C083745:SLC1 protein, S cerevisiae