Association of DLC1 gene polymorphism with susceptibility to hepatocellular carcinoma in Chinese hepatitis B virus carriers.
BACKGROUND
Lost or downexpression of the gene deleted in liver cancer 1 (DLC1) has been implicated in the development of hepatocellular carcinoma (HCC). We examined the relationship between DLC1 polymorphisms and HCC risk among Chinese.


METHODS
Three DLC1 polymorphisms, Ex11+255T>G (rs3739298), Ex11-620G>A (rs532841) and IVS19+108C>T (rs621554), were genotyped in 434 patients with HCC and 480 controls by PCR-RFLP. The associations with the susceptibility to HCC were evaluated while controlling for confounding factors.


RESULTS
We observed significantly increased susceptibility to HCC for the C/C genotype compared with T/T of IVS19+108C>T in the HBV carriers (OR=2.95, 95% CI, 1.65-5.26, P<0.001). Compared with the haplotype G-A-T (in order of Ex11+255T>G, Ex11-620G>A and IVS19+108C>T), the haplotype T-G-C was also significantly associated with an increased susceptibility to HCC among HBV carriers (OR=2.16, 95% CI, 1.08-4.35, P=0.009). The stratified analysis indicated no modification of the confounding factors on the increased susceptibility to HCC related to the DLC1 polymorphism/haplotype.


CONCLUSIONS
Our findings suggest that DLC1 genetic polymorphism or haplotype play a role in mediating the susceptibility to HBV-related HCC.
Dong
Xiaoqun
X
The State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.
Zhou
Gangqiao
G
Zhai
Yun
Y
Zhang
Hongxing
H
Yang
Hao
H
Zhi
Lianteng
L
Zhang
Xiumei
X
Chu
Jiayou
J
He
Fuchu
F
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000821:virology; D002353:Carrier State; D002681:China; D005260:Female; D020690:GTPase-Activating Proteins; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D025521:Tumor Suppressor Proteins / Q000235:genetics*
C112352:DLC1 protein, human; D020690:GTPase-Activating Proteins; D025521:Tumor Suppressor Proteins