[Optimal extraction of melampyrit from Euonymus fortunei by central composite design-response surface methodology].
OBJECTIVE
To optimize the process of extracting melampyrit from Euonymus fortunei by central composite design-response surface methodology.


METHODS
The independent variables were the solvent fold and extractive time, and the dependent variable was the extraction rate of melampyrit from Euonymus fortunei. Then different mathematic models were used to estimate the relationship between the independent and dependent variables. The response surface methodology was used to optimize the process of extraction and the prediction was carried out through comparing the observed and predicted values.


RESULTS
The regression coefficient of binomial fitting complex model was 0.9515, and the optimum conditions of extraction process were 12-fold volume of solvent, 1.5 hours for decoction and 2 times for extraction. The bias between the observed and predicted values was -5.37%.


CONCLUSIONS
It shows that this method is convenient and the optimum model is highly predictive.
Wei
Wen-Jun
WJ
Faculty of Phamacy, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China. wwj5101@hotmail.com
Liu
Yan-Ping
YP
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029756:Euonymus / Q000737:chemistry*; D004376:Galactitol / Q000032:analysis / Q000302:isolation & purification*; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D012997:Solvents; D014867:Water; D004376:Galactitol