title: WNT5A gene is aberrantly methylated from the early stages of colorectal cancers.
abstract: OBJECTIVE
  Recently, it has been reported that WNT5A methylation was frequently detected in colorectal cancers. However, the relationship between the WNT5A methylation and the characteristics of these cancers remains unknown.
  
  
  METHODS
  Methylation status of the WNT5A gene was examined in primary carcinomas and the corresponding normal tissues derived from 49 patients with colorectal cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.
  
  
  RESULTS
  Aberrant methylation of the WNT5A gene was detected in 17 out of the 49 (35%) primary colorectal cancers. The clinicopathological data were correlated with the methylation results. All stages of colorectal cancers presented WNT5A methylation.
  
  
  CONCLUSIONS
  WNT5A gene has been methylated from the early stages of colorectal cancers.
authors.affiliation: Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama 227-8501, Japan. kenjih-ngy@umin.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Hibi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizukami
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Goto
authors.forename: Youhei
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Makiko
authors.initials: M
authors.lastname: Sakata
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Saito
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Gaku
authors.initials: G
authors.lastname: Kigawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nemoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sanada
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051153:Wnt Proteins / Q000235:genetics*; D000071818:Wnt-5a Protein
chemical_list: D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein