Tateyamaria pelophila sp. nov., a facultatively anaerobic alphaproteobacterium isolated from tidal-flat sediment, and emended descriptions of the genus Tateyamaria and of Tateyamaria omphalii.
A Gram-negative motile rod, strain SAM4T, was isolated from the highest positive dilution of a most probable number series inoculated with tidal-flat sediments from the German North Sea coast. The isolate grew at 4-35 degrees C and showed constant growth yields throughout almost the whole temperature range. Growth was observed between pH 6 and 9 and at salinities of 0.3-10.2%. Strain SAM4T required Na+ for growth, contained bacteriochlorophyll a and was catalase- and oxidase-positive. It was nutritionally versatile growing on a variety of carbon compounds including carbohydrates, amino acids and organic acids like lactate or succinate. It grew anaerobically on complex media such as marine broth, indicating fermentation, and by reducing trimethylammonium oxide. The dominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol, whereas only traces of phosphatidylcholine and an unidentified lipid were found. The major fatty acid was n-C18:1omega7c. The DNA G+C content was 56.4 mol%. The isolate was identified as a member of the Roseobacter clade within the class Alphaproteobacteria. However, based on phylogenetic, phenotypic and physiological data, it clearly differs from its closest relative Tateyamaria omphalii. Therefore, a novel species is proposed: Tateyamaria pelophila sp. nov., with strain SAM4T (=DSM 17270T=LMG 23018T) as the type strain. Emended descriptions of the genus Tateyamaria and of Tateyamaria omphalii are also presented.
Sass
Henrik
H
School of Earth and Ocean Sciences, Cardiff University, Cardiff CF10 3YE, Wales, UK.|Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Straße 9-11, D-26111 Oldenburg, Germany.
Köpke
Beate
B
Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Straße 9-11, D-26111 Oldenburg, Germany.
Rütters
Heike
H
Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Straße 9-11, D-26111 Oldenburg, Germany.
Feuerlein
Theresa
T
Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Straße 9-11, D-26111 Oldenburg, Germany.
Dröge
Stefan
S
Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Straße 9-11, D-26111 Oldenburg, Germany.
Cypionka
Heribert
H
Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Straße 9-11, D-26111 Oldenburg, Germany.
Engelen
Bert
B
Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Straße 9-11, D-26111 Oldenburg, Germany.
D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S