title: Genistein inhibition of topoisomerase IIalpha expression participated by Sp1 and Sp3 in HeLa cell.
abstract: Genistein (4', 5, 7-trihydroxyisoflavone) is an isoflavone compound obtained from plants that has potential applications in cancer therapy. However, the molecular mechanism of the action of genistein on cancer cell apoptosis is not well known. In this study, we investigated the effect of genistein on topoisomerase II-alpha (Topo IIalpha), an important protein involved in the processes of DNA replication and cell proliferation. The results revealed that inhibition of Topo IIalpha expression through the regulation of Specificity protein 1 and Specificity protein 3 may be one of the reasons for genistein's induction of HeLa cell apoptosis.
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Najing
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Yunli
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Li
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Han
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Chang'an District Hospital, Shijiazhuang 050017, Hebei, China.
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Li
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D019833:Genistein / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D051705:Sp3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D016588:Anticarcinogenic Agents; D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D051705:Sp3 Transcription Factor; D019833:Genistein; D004250:DNA Topoisomerases, Type II