title: The RNA-binding protein NANOS2 is required to maintain murine spermatogonial stem cells.
abstract: Stem cells give rise to differentiated cell types but also preserve their undifferentiated state through cell self-renewal. With the use of transgenic mice, we found that the RNA-binding protein NANOS2 is essential for maintaining spermatogonial stem cells. Lineage-tracing analyses revealed that undifferentiated spermatogonia expressing Nanos2 self-renew and generate the entire spermatogenic cell lineage. Conditional disruption of postnatal Nanos2 depleted spermatogonial stem cell reserves, whereas mouse testes in which Nanos2 had been overexpressed accumulated spermatogonia with undifferentiated, stem cell-like properties. Thus, NANOS2 is a key stem cell regulator that is expressed in self-renewing spermatogonial stem cells and maintains the stem cell state during murine spermatogenesis.
authors.affiliation: Department of Genetics, SOKENDAI, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Sada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Hitomi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saga
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D055786:Gene Knockout Techniques; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D016601:RNA-Binding Proteins / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002352:Carrier Proteins; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D051741:Kruppel-Like Transcription Factors; D009419:Nerve Tissue Proteins; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D016601:RNA-Binding Proteins; C494988:Gfra1 protein, mouse; C477341:Nanos2 protein, mouse; C477342:Nanos3 protein, mouse; C407737:Neurog3 protein, mouse; C513267:Zbtb16 protein, mouse