Structural effects of clinically observed mutations in JAK2 exons 13-15: comparison with V617F and exon 12 mutations.
BACKGROUND
The functional relevance of many of the recently detected JAK2 mutations, except V617F and exon 12 mutants, in patients with chronic myeloproliferative neoplasia (MPN) has been significantly overlooked. To explore atomic-level explanations of the possible mutational effects from those overlooked mutants, we performed a set of molecular dynamics simulations on clinically observed mutants, including newly discovered mutations (K539L, R564L, L579F, H587N, S591L, H606Q, V617I, V617F, C618R, L624P, whole exon 14-deletion) and control mutants (V617C, V617Y, K603Q/N667K).


RESULTS
Simulation results are consistent with all currently available clinical/experimental evidence. The simulation-derived putative interface, not possibly obtained from static models, between the kinase (JH1) and pseudokinase (JH2) domains of JAK2 provides a platform able to explain the mutational effect for all mutants, including presumably benign control mutants, at the atomic level.


CONCLUSIONS
The results and analysis provide structural bases for mutational mechanisms of JAK2, may advance the understanding of JAK2 auto-regulation, and have the potential to lead to therapeutic approaches. Together with recent mutation profiling results demonstrating the breadth of clinically observed JAK2 mutations, our findings suggest that molecular testing/diagnostics of JAK2 should extend beyond V617F and exon 12 mutations, and perhaps should encompass most of the pseudo-kinase domain-coding region.
Lee
Tai-Sung
TS
Biomedical Informatics and Computational Biology, and Department of Chemistry, University of Minnesota, 207 Pleasant Street, S.E., Minneapolis, MN 55455, USA. leex2750@umn.edu
Ma
Wanlong
W
Zhang
Xi
X
Kantarjian
Hagop
H
Albitar
Maher
M
D019295:Computational Biology; D005091:Exons; D006801:Humans; D053614:Janus Kinase 2 / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D009154:Mutation; D010649:Phenylalanine / Q000235:genetics; D017434:Protein Structure, Tertiary; D040681:Structural Homology, Protein; D014633:Valine / Q000235:genetics
D010649:Phenylalanine; D053614:Janus Kinase 2; D014633:Valine