title: Oligomeric formylpeptide activity on human neutrophils.
abstract: A series of oligomeric formylpeptides were synthesized by cross-linking the prototype fMLP using a Lys residue. They were then investigated for their ability to stimulate chemotaxis, superoxide anion production, and lytic enzyme release in human neutrophils. Although active in stimulating the different receptor isoforms, leading to the different biological responses, these analogues showed a lesser potency and affinity than the standard peptide. On the basis of the results reported here, we can hypothesise that: (i) the increased bulk of these molecules seems to hinder their correct positioning into the receptor pocket, thereby hindering favourable receptor interaction; and that: (ii) fMLP space positions do not seem to allow the ligand to increase biological responses.
authors.affiliation: Department of Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara 17/19, Ferrara 44100, Italy. g5z@unife.it
authors.forename: Giorgio
authors.initials: G
authors.lastname: Cavicchioni
authors.forename: Anna
authors.initials: A
authors.lastname: Fraulini
authors.forename: Sofia
authors.initials: S
authors.lastname: Falzarano
authors.forename: Susanna
authors.initials: S
authors.lastname: Spisani
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D010455:Peptides; D013481:Superoxides