title: Development of a rapid and reversible photocrosslinking of RNA.
abstract: We describe a novel reversible interstrand photocrosslinking reaction of RNA. In this system, a modified oligodeoxynucleotide (ODN) containing 3-cyanovinylcarbazole nucleoside ((CNV)K) reacts by photoirradiation at 366 nm for 1 s with pyrimidine residue of a complementary template RNA to yield a crosslinked product in 94% yield.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan.
authors.forename: Yoshinaga
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ohtake
authors.forename: Hajime
authors.initials: H
authors.lastname: Okada
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
mesh_terms: D002227:Carbazoles / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects; D055668:Photochemical Processes; D012313:RNA / Q000737:chemistry* / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002227:Carbazoles; D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA