Photoelectrochemical identification of 5-methylcytosine modification in DNA: combination of photosensitization and enzymatic cleavage.
We present a novel photoelectrochemical approach for discriminating between cytosine and 5-methylcytosine in DNA when used in combination with enzymatic digestion. A photosensitizer-linked DNA duplex bearing 5-methylcytosine or cytosine at a given restriction site of the strand was immobilized on a gold electrode and digested with enzyme, and the photocurrent response was measured. We observed high photocurrent density that was selective for the methylated duplex.
Tanabe
Kazuhito
K
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan. tanabeka@scl.kyoto-u.ac.jp
Yamada
Hisatsugu
H
Ito
Takeo
T
Nishimoto
Sei-ichi
S
D044503:5-Methylcytosine / Q000032:analysis* / Q000737:chemistry; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D004262:DNA Restriction Enzymes; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D055668:Photochemical Processes; D010777:Photochemistry / Q000379:methods
D009838:Oligodeoxyribonucleotides; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D004262:DNA Restriction Enzymes