title: Alpha-N-acetylglucosamine-linked O-glycans of sialoglycoproteins (Tc-mucins) from Trypanosoma cruzi Colombiana strain.
abstract: Trypanosoma cruzi sialoglycoproteins (Tc-mucins) are mucin-like molecules linked to a parasite membrane via a glycosylphosphatidylinositol anchor. We previously determined the structures of Tc-mucin O-glycan domains from several T. cruzi strains and observed significant differences among them. We now report the amino acid content and structure of Tc-mucin O-glycan chains from T. cruzi Colombiana, a strain resistant to common trypanocidal drugs. Amino acid analysis demonstrated the predominance of threonine residues (42%) and helped to identify the O-glycans as belonging to a Tc-mucin family that contain a beta-galactofuranose (beta-Galf) residue attached to an alpha-N-acetylglucosamine (alpha-GlcNAc) O-4, with the most complex glycan, a pentasaccharide-GlcNAc-ol with a branched trigalactopyranose chain, on the GlcNAc O-6. The presence of beta-Galf on O-glycans from T. cruzi Colombiana mucins supports the use of glycosylation as a phylogenetic marker for the classification of Colombiana in the T. cruzi I group.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Adriane R
authors.initials: AR
authors.lastname: Todeschini
authors.forename: Eliane G
authors.initials: EG
authors.lastname: de Almeida
authors.forename: Orlando A
authors.initials: OA
authors.lastname: Agrellos
authors.forename: Christopher
authors.initials: C
authors.lastname: Jones
authors.forename: José O
authors.initials: JO
authors.lastname: Previato
authors.forename: Lucia
authors.initials: L
authors.lastname: Mendonça-Previato
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis*; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D009077:Mucins / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis*; D012795:Sialoglycoproteins / Q000032:analysis*; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000145:classification
chemical_list: D009077:Mucins; D009844:Oligosaccharides; D012795:Sialoglycoproteins; D000117:Acetylglucosamine