[Sensitivity of interferon-gamma release assay T-SPOT. TB in diagnosing extrapulmonary tuberculosis].
OBJECTIVE
To evaluate the sensitivity of an interferon-gamma release assay T-SPOT. TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis.


METHODS
Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT. TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells (PBMCs).


RESULTS
T-SPOT. TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5% (95% CI 84.8% - 100%). The median of spot forming cells (SFCs) in response to early secreting antigen target 6 peptides was 196/10(6) PBMCs (interquartile range, 72-532/10(6) PBMCs), the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/10(6) PBMCs (interquartile range, 72-568/10(6) PBMCs), and the median of the incorporate SFCs was 612/10(6) PBMCs (interquartile range, 192-1 152/10(6) PBMCs).


CONCLUSIONS
T-SPOT. TB is highly sensitive in diagnosing extrapulmonary tuberculosis.
Huo
Fei-Fei
FF
Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zhang
Li-Fan
LF
Liu
Xiao-Qing
XQ
D006801:Humans; D007371:Interferon-gamma; D059425:Interferon-gamma Release Tests; D007963:Leukocytes, Mononuclear; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*
D007371:Interferon-gamma