Effect of vitamin A supplemented diet on calcium oxalate renal stone formation in rats.
OBJECTIVE
To study the effect of a vitamin A supplemented diet on calcium-oxalate stone formation in rats and to test its expected action in the dissolution of renal calculi.


METHODS
Twenty-four male Wistar rats were randomly divided into three groups of eight rats each. The first group (group A) received a normal diet for six weeks. The second group (group B) was fed a lithogenic diet by the addition of ethylene glycol 0.5% to drinking water for three weeks then a normal diet for three weeks. The third group (group C) received the same lithogenic diet for three weeks then a vitamin A supplemented diet 20 times the normal amount (5.1mg/100g of diet) at the three last weeks. One day before the end of treatment, each animal was placed for 24h in metabolic cage in order to collect urine samples and determine the urinary parameters.


RESULTS
The glomerular filtration rate and the urinary excretion of citric acid which fell in group B have been restored in group C.


CONCLUSIONS
This study shows that a vitamin A supplemented diet at the rate of 20 times standard ration could improve the renal function by restoring the glomerular filtration rate and by increasing the urinary pH and excretion of citric acid.
Bardaoui
Mourad
M
Laboratoire de Physiologie, Faculté de Médecine, 04/UR/09-03, Monastir 5000, Tunisia. bardaouimourad@yahoo.fr
Sakly
Rachid
R
Neffati
Fadoua
F
Najjar
Mohamed Fadel
MF
El Hani
Abdelhamid
A
D000818:Animals; D002129:Calcium Oxalate / Q000378:metabolism*; D019343:Citric Acid / Q000652:urine; D019587:Dietary Supplements; D019855:Ethylene Glycol / Q000633:toxicity; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007669:Kidney Calculi / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014801:Vitamin A / Q000627:therapeutic use*
D014801:Vitamin A; D002129:Calcium Oxalate; D019343:Citric Acid; D019855:Ethylene Glycol