title: [Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus].
abstract: The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder, Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63% of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ni
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Guang-Qing
authors.initials: GQ
authors.lastname: Liu
authors.forename: Liu
authors.initials: L
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Tao
authors.initials: T
authors.lastname: Yun
authors.forename: Jiong-Gang
authors.initials: JG
authors.lastname: Hua
authors.forename: Shuang-Mao
authors.initials: SM
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D017250:Caliciviridae Infections / Q000235:genetics / Q000503:physiopathology* / Q000662:veterinary* / Q000821:virology; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000821:virology; D053938:DNA Fragmentation; D017929:Hemorrhagic Disease Virus, Rabbit / Q000502:physiology*; D011817:Rabbits
chemical_list: D053148:Caspase 3