title: Comparison of the activities of bacterial and mammalian nucleotide excision repair systems for DNA-protein crosslinks.
abstract: Endogenous and environmental genotoxic agents produce DNA damage and induce cell death and mutations. The repair mechanisms of base lesions and single and double strand breaks have been well characterized in both prokaryotic and eukaryotic cells. However, the molecular pathways that repair or tolerate DNA-protein crosslinks (DPCs) remains to be largely elucidated. In this study, we constructed DNA substrates containing defined DPCs and assessed the incision activities of prokaryotic and eukaryotic nucleotide excision repair systems for DPCs in vitro.
authors.affiliation: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakano
authors.forename: Amir M H
authors.initials: AM
authors.lastname: Salem
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Terato
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ide
mesh_terms: D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D011506:Proteins / Q000737:chemistry
chemical_list: D029968:Escherichia coli Proteins; D011506:Proteins; D004247:DNA; D004706:Endodeoxyribonucleases; C036385:endodeoxyribonuclease uvrABC