title: [Effects of ERK and STAT3 on cell signal transduction in alveolar epithelium A549 cells stimulated by mechanical force].
abstract: OBJECTIVE
  To observe the effect of mechanical force on the expression of p-ERK and p-STAT3 when pulmonary epithelium A549 cells were subjected to tensile stress.
  
  
  METHODS
  By the self-made four-point bending system, the A549 cells were subjected to cyclic tensile stress of 3000 microstrain for 30 min, 1 h, 2 h or 4 h respectively, then the total protein of each time point was collected and the expression of p-ERK and p-STAT3 were detected by Western blot analysis.
  
  
  RESULTS
  (1) The expression of p-ERK was activated at 1 h and remained at a high level until 4 h. (2) The expression of p-STAT3 (Ser) was activated at 30 min and remained at a high level until 4 h.
  
  
  CONCLUSIONS
  Tensile stress can activate the expression of p-ERK and p-STAT3 (Ser) in alveolar epithelium A549 cells. ERK and STAT3 may participate in the cell mechanotransduction in A549 cells.
authors.affiliation: Department of Respiratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jing
authors.initials: J
authors.lastname: An
authors.forename: Su-Xia
authors.initials: SX
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Hou
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Hu
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Wen
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D011650:Pulmonary Alveoli / Q000166:cytology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D013314:Stress, Mechanical
chemical_list: D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D048049:Extracellular Signal-Regulated MAP Kinases