Investigation of the mechanism of the fluorescent emission of the silylated pyrene modified DNA.
Modified DNA containing silylated pyrene derivative at the 5'-terminus showed the obvious discrimination ability of the duplex formation, that the modified DNA showed the strong fluorescence emission in the presence of the complementary DNA, in spite of the very weak emission in the absence of the complementary DNA. The intensity of the fluorescence emission of the double strand found to increase about 10 times compared with that of the single strand. In this paper, we tried to disclose the detailed mechanism of such a discrimination ability of the silylated pyrene-modified DNA.
Moriguchi
Tomohisa
T
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan.
Hattori
Masayoshi
M
Sekiguchi
Tohru
T
Shinozuka
Kazuo
K
D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D017646:Organosilicon Compounds; D011721:Pyrenes; D004247:DNA