[Heterotopic complex membrane induces bone formation].
OBJECTIVE
The composite membrane was made by recombinant human bone morphogenetic protein-2 (rhBMP-2), collagen (Co), polylactic-co-glycolic acid (PLGA). To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane.


METHODS
48 male Kunming adult mice were divided into two groups randomly: Co/PLGA complex membrane group and rhBMP-2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination.


RESULTS
Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft X-ray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane.


CONCLUSIONS
Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.
Lin
Chong-tao
CT
Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China.
Li
Hong-yan
HY
Yan
Jia-qing
JQ
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D003094:Collagen; D006016:Glycolates; D019344:Lactic Acid; D008297:Male; D051379:Mice; D010012:Osteogenesis; D011091:Polyesters; D011100:Polyglycolic Acid; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta
D055396:Bone Morphogenetic Protein 2; D006016:Glycolates; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; C031149:glycolic acid; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen