Isolation of a Bacillus strain producing ketone reductase with high substrate tolerance.
Target reaction-oriented screening from soil samples yielded a ketone reductase-producing Bacillus sp., strain ECU0013, which exhibits excellent stereoselectivity, high substrate tolerance and is capable of regenerating the required cofactor with glucose as a co-substrate. Whole-cells catalyzed the asymmetric reduction of 2-chloro-1-phenylethanone (50mM) to (R)-2-chloro-1-phenylethanol with a 93.3% conversion rate and 99% e.e. (enantiomeric excess). A variety of ketones were enantioselectively reduced by resting cells, giving corresponding chiral alcohols with good to excellent e.e. values. These results suggest the potential of this strain for the industrial production of chiral halogenated aromatic alcohols.
Xie
Yan
Y
Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Xu
Jian-He
JH
Xu
Yi
Y
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000438:Alcohols / Q000737:chemistry; D001407:Bacillus / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D013237:Stereoisomerism; D013379:Substrate Specificity; D013997:Time Factors; D002721:omega-Chloroacetophenone / Q000737:chemistry
D000438:Alcohols; D002721:omega-Chloroacetophenone; D000429:Alcohol Oxidoreductases; D005947:Glucose