title: New quantification method for estradiol in the prostatic tissues of benign prostatic hyperplasia using liquid chromatography-tandem mass spectrometry.
abstract: Estrogen is suspected to play a role in the pathogenesis of benign prostatic hyperplasia (BPH) and prostate cancer. To clarify the role of estradiol (E2) in the prostatic tissues (prostatic tissue E2) during the development of prostatic disorders, we developed a new sensitive and specific quantification method for prostatic tissue E2 using liquid chromatography-tandem mass spectrometry (LC-MS/MS). For the solid-phase extraction, E2 was purified by anion-exchange through an Oasis MAX cartridge. In addition, after the formation of 3-pentaflurobenzyl-17beta-pyridinium-estradiol derivative (E2-PFBPY), E2-PFBPY was purified by cation-exchange through an Oasis WCX cartridge. These processes in the LC-MS/MS method improved the specificity and sensitivity for prostatic tissue E2 measurement, compared to the radioimmunoassay (RIA) method. The validation tests showed that intra-day and inter-day precisions were both within +/-15% (except for 15.5% of the inter-day precision of the lowest concentration), with the accuracy ranging from 88 to 110%. The quantification limit of this assay was 0.15pg/tube in our method, which was 80-fold more sensitive than that of the RIA method. With the use of our present method, the median E2 levels in the prostatic tissues in patients with BPH (n=20, median age: 71 years) were 12.0pg/g tissue (95% confidence interval=9.1-22.6pg/g tissue). Furthermore, the E2 levels increased significantly with aging. These results showed that our present method would be useful for elucidating the role of prostatic tissue E2 in the development of prostatic disorders with a small amount of tissue samples.
authors.affiliation: Department of Urology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi 371-8511, Japan. seiji@showa.gunma-u.ac.jp
authors.forename: Seiji
authors.initials: S
authors.lastname: Arai
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Miyashiro
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Shibata
authors.forename: Bunzo
authors.initials: B
authors.lastname: Kashiwagi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Tomaru
authors.forename: Mikio
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Seijiro
authors.initials: S
authors.lastname: Honma
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004958:Estradiol / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D006801:Humans; D008297:Male; D011467:Prostate / Q000737:chemistry* / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004958:Estradiol