title: Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization.
abstract: The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.
authors.affiliation: ForteBio, Inc, CA 94025, USA. concepcionjoy@netscape.net
authors.forename: Joy
authors.initials: J
authors.lastname: Concepcion
authors.forename: Krista
authors.initials: K
authors.lastname: Witte
authors.forename: Charles
authors.initials: C
authors.lastname: Wartchow
authors.forename: Sae
authors.initials: S
authors.lastname: Choo
authors.forename: Danfeng
authors.initials: D
authors.lastname: Yao
authors.forename: Henrik
authors.initials: H
authors.lastname: Persson
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: Pu
authors.initials: P
authors.lastname: Li
authors.forename: Bettina
authors.initials: B
authors.lastname: Heidecker
authors.forename: Weilei
authors.initials: W
authors.lastname: Ma
authors.forename: Ram
authors.initials: R
authors.lastname: Varma
authors.forename: Lian-She
authors.initials: LS
authors.lastname: Zhao
authors.forename: Donald
authors.initials: D
authors.lastname: Perillat
authors.forename: Greg
authors.initials: G
authors.lastname: Carricato
authors.forename: Michael
authors.initials: M
authors.lastname: Recknor
authors.forename: Kevin
authors.initials: K
authors.lastname: Du
authors.forename: Huddee
authors.initials: H
authors.lastname: Ho
authors.forename: Tim
authors.initials: T
authors.lastname: Ellis
authors.forename: Juan
authors.initials: J
authors.lastname: Gamez
authors.forename: Michael
authors.initials: M
authors.lastname: Howes
authors.forename: Janette
authors.initials: J
authors.lastname: Phi-Wilson
authors.forename: Scott
authors.initials: S
authors.lastname: Lockard
authors.forename: Robert
authors.initials: R
authors.lastname: Zuk
authors.forename: Hong
authors.initials: H
authors.lastname: Tan
mesh_terms: D000906:Antibodies / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055808:Drug Discovery / Q000379:methods; D007368:Interferometry / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D011506:Proteins / Q000737:chemistry; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D000906:Antibodies; D008024:Ligands; D011506:Proteins