Production of D-arabitol by a newly isolated Kodamaea ohmeri.
A new yeast, isolated from natural osmophilic sources, produces D-arabitol as the main metabolic product from glucose. According to 18S rRNA analysis, the NH-9 strain belongs to the genus Kodamaea. The optimal culture conditions for inducing production of D-arabitol were 37 degrees C, neutral pH, 220 rpm shaking, and 5% inoculum. The yeast produced 81.2 +/- 0.67 g L(-1) D-arabitol from 200 g L(-1) D-glucose in 72 h with a yield of 0.406 g g(-1) glucose and volumetric productivity Q(P) of 1.128 g L(-1) h(-1). Semi-continuous repeated-batch fermentation was performed in shaker-flasks to enhance the process of D-arabitol production by Kodamaea ohmeri NH-9 from D-glucose. Under repeated-batch culture conditions, the highest volumetric productivity was 1.380 g L(-1) h(-1).
Zhu
Hong-Yang
HY
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Gulou District, Nanjing, China.
Xu
Hong
H
Dai
Xiao-Yan
XY
Zhang
Yang
Y
Ying
Han-Jie
HJ
Ouyang
Ping-Kai
PK
D005947:Glucose / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism*
D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D013402:Sugar Alcohols; D005947:Glucose; C014999:arabitol