[Effects of tumor necrosis factor-alpha on the growth of rat osteoblasts].
OBJECTIVE
To investigate the effects of tumor necrosis factor alpha (TNF-alpha) on the growth of rat osteoblasts. To find out the mechanisms that TNF-alpha regulates the growth of osteoblasts.


METHODS
To assay osteoblasts proliferation by MTT. To assay alkaline phosphatase (ALP) activity of osteoblasts by PP-nitrophenyl phosphate (PNPP).


RESULTS
The osteblasts proliferation and the ALP activity decreased in treatment groups, and the significantly lower levels were observed in above 50 ng/mL groups (P<0.05).


CONCLUSIONS
TNF-alpha restrained osteoblasts proliferation and differentiation, and the effects were more significant in above 50 ng/mL groups.
Xue
Li-wei
LW
Dept. of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan 250012, China.
Zhang
Jun
J
Wang
Xu-xia
XX
Bu
Tao
T
Liu
Mei
M
D000469:Alkaline Phosphatase; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D010006:Osteoblasts; D051381:Rats; D014409:Tumor Necrosis Factor-alpha
D014409:Tumor Necrosis Factor-alpha; D000469:Alkaline Phosphatase