title: Biological activities of sulfated polysaccharides from tropical seaweeds.
abstract: Sulfated polysaccharides from 11 species of tropical marine algae (one edible specie of Rhodophyta, six species of Phaeophyta and four species of Chlorophyta) collected from Natal city coast (Northeast of Brazil) were evaluated for their anticoagulant, antioxidant and antiproliverative in vitro activities. In the activated partial thromboplastin time (APTT) test, which evaluates the intrinsic coagulation pathway, seven seaweeds presented anticoagulant activity. Dictyota cervicornis showed the highest activity, prolonging the coagulation time to double the baseline value in the APTT with only 0.01 mg/100 microl of plasma, 1.4-fold lesser than Clexane, a low molecular weight heparin. In the protrombin time (PT) test, which evaluates the extrinsic coagulation pathway, only Caulerpa cupresoides showed anticoagulant activity. All species collected showed antioxidant activities. This screening emphasized the great antioxidant potential (total capacity antioxidant, power reducing and ferrous chelating) of four species: C. sertularioide; Dictyota cervicornis; Sargassum filipendula and Dictyopteris delicatula. After 72 h incubation, HeLa cell proliferation was inhibited (p<0.05) between 33.0 and 67.5% by S. filipendula; 31.4 and 65.7% by D. delicatula; 36.3 and 58.4% by Caulerpa prolifera and 40.2 and 61.0% by Dictyota menstrualis at 0.01-2mg/mL algal polysaccharides. The antiproliferative efficacy of these algal polysaccharides were positively correlated with the sulfate content (r=0.934). Several polysaccharides demonstrated promising antioxidant, antiproliferative an/or anticoagulant potential and have been selected for further studies on bioguided fractionation, isolation and characterization of pure polysaccharides from these species as well as in vivo experiments are needed and are already in progress.
authors.affiliation: UFRN, Laboratório de Biotecnologia de Polímeros Naturais-Biopol, Centro de Biociências, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho, 3000, 59072970 Natal, Rio Grande do Norte, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Costa
authors.forename: G P
authors.initials: GP
authors.lastname: Fidelis
authors.forename: S L
authors.initials: SL
authors.lastname: Cordeiro
authors.forename: R M
authors.initials: RM
authors.lastname: Oliveira
authors.forename: D A
authors.initials: DA
authors.lastname: Sabry
authors.forename: R B G
authors.initials: RB
authors.lastname: Câmara
authors.forename: L T D B
authors.initials: LT
authors.lastname: Nobre
authors.forename: M S S P
authors.initials: MS
authors.lastname: Costa
authors.forename: J
authors.initials: J
authors.lastname: Almeida-Lima
authors.forename: E H C
authors.initials: EH
authors.lastname: Farias
authors.forename: E L
authors.initials: EL
authors.lastname: Leite
authors.forename: H A O
authors.initials: HA
authors.lastname: Rocha
mesh_terms: D000925:Anticoagulants / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D017984:Enoxaparin / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D010314:Partial Thromboplastin Time; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011517:Prothrombin Time; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D017984:Enoxaparin; D011134:Polysaccharides