Monitoring insulin-stimulated production of signaling lipids at the plasma membrane.
Lipid second messengers play important roles in many cell signaling cascades. Lipid signaling molecules allow for high specificity, rapid transduction, and rapid reversibility of localized stimulation events. Fluorescent sensors capable of detecting individual signaling lipids enable their production and degradation to be followed, revealing the nature and dynamics of signaling pathways. The following sections outline a method for using lipid sensors to monitor the production of signaling lipids on the plasma membrane of C2C12 myotubes in response to insulin signaling.
Osisami
Mary
M
Center for Developmental Genetics, Graduate Program in Genetics, Stony Brook University, Stony Brook, NY, USA.
Huang
Huiyan
H
Frohman
Michael A
MA
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007328:Insulin / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D051379:Mice; D018613:Microscopy, Confocal; D015290:Second Messenger Systems; D015398:Signal Transduction / Q000187:drug effects*
D007328:Insulin; D008055:Lipids