title: Circulating beta chemokine and MMP 9 as markers of oxidative injury in extremely low birth weight infants.
abstract: Matrix metalloproteinases (MMPs) and chemokines seem to be induced by hyperoxia in preclinical studies. We hypothesized that O2 exposure immediately after birth is associated with altered blood spot MMP 9 and beta chemokine concentrations. The following analytes were measured on blood spots on d 1 and 3 of life, using luminex technology in 1059 infants (birth weights <1000 g) in the NICHD Neonatal Research Network: MMP 9, monocyte chemoattractant protein 1 (MCP 1), macrophage inflammatory proteins (1alpha and beta), and regulated upon activation, normal t cell expressed and secreted (RANTES). Infants administered O2 continually from 6 to 24 h of life (n = 729), when compared with those with <6 h exposure (n = 330), had significantly lower mean birth weight and higher rate of respiratory distress syndrome (p < 0.002). On d 3, MCP 1 was higher and RANTES lower among infants with early prolonged O2 exposure. After adjusting for covariates, prolonged early O2 exposure retained a statistically significant association with higher MCP 1 on d 3 (p = 0.003). The consistent association between O2 exposure and MCP 1 among extremely preterm infants suggests that further investigation of its role in oxidative injury is warranted.
authors.affiliation: Department of Pediatrics, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. gnatara@med.wayne.edu
authors.forename: Girija
authors.initials: G
authors.lastname: Natarajan
authors.forename: Seetha
authors.initials: S
authors.lastname: Shankaran
authors.forename: Scott A
authors.initials: SA
authors.lastname: McDonald
authors.forename: Abhik
authors.initials: A
authors.lastname: DAS
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Stoll
authors.forename: Rosemary D
authors.initials: RD
authors.lastname: Higgins
authors.forename: Poul
authors.initials: P
authors.lastname: Thorsen
authors.forename: Kristin
authors.initials: K
authors.lastname: Skogstrand
authors.forename: David M
authors.initials: DM
authors.lastname: Hougaard
authors.forename: Waldemar A
authors.initials: WA
authors.lastname: Carlo
mesh_terms: D019742:Chemokines, CC / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D052577:Infant, Extremely Low Birth Weight; D007231:Infant, Newborn; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*
chemical_list: D019742:Chemokines, CC; D020780:Matrix Metalloproteinase 9