Molecular characterization of human parvovirus B19 genotypes 2 and 3.
We have characterized the transcription profiles of parvovirus B19 (B19V) genotype-2 A6 and genotype-3 V9 variants. The A6 RNA profile differs from that of the prototype B19V in both B19V non-permissive and permissive cells, whereas the overall profile of the V9 RNA in these cells is similar to that of the prototype. A unique feature we have identified is that the genotype-2 A6 variant used only one splice acceptor to remove the first intron. We also demonstrated that the inverted terminal repeats (ITRs) of the prototype B19V support replication of the V9 genome, which produces infectious virus, but not that of the A6 genome, in B19V-permissive cells. Similar to the proapoptotic nature of the prototype B19V large non-structural protein (NS1), the A6 and V9 NS1 proteins also are potent inducers of apoptosis in B19V-permissive cells.
Chen
Zhaojun
Z
Department of Clinical Laboratory, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, China.
Guan
Wuxiang
W
Cheng
Fang
F
Chen
Aaron Yun
AY
Qiu
Jianming
J
D017209:Apoptosis; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020079:Terminal Repeat Sequences; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology
D017931:DNA Primers; C106202:NS1 protein, parvovirus; D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins