title: [Detection of specific interferon-gamma-secreting T cell response to Mycobacterium tuberculosis RD1-encoded antigens in pleural effusions, ascites, and cerebrospinal fluid].
abstract: OBJECTIVE
  To detect Mycobacterium tuberculosis RD1-encoded antigens-specific, interferon-gamma (INF-gamma)-secreting T cells in pleural effusions, ascites, and cerebrospinal fluid.
  
  
  METHODS
  The early secretory antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) peptides-specific T cells in peripheral blood mononuclear cell (MC), ascites MC, pleural effusions MC, and cerebrospinal fluid MC were detected using enzyme-linked immunospot assay (ELISPOT) for INF-gamma.
  
  
  RESULTS
  ESAT-6 or CFP-10 peptides-specific, INF-gamma-secreting T cells were detected in peripheral blood, ascites, pleural effusions, and cerebrospinal fluid, which marked the presence of tuberculosis infection. Patients with positive ELISPOT results of INF-gamma-release assay were all diagnosed as active tuberculosis. Spot forming cells in ascites and pleural effusions were much higher than those in peripheral blood (up to 6.4 and 31.9 times).
  
  
  CONCLUSIONS
  Detection of RD1-encoded antigens-specific, INF-gamma-secreting T cells in pleural effusions, ascites, and cerebrospinal fluid provides a new way to diagnose tuberculosis infection.
authors.affiliation: Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Zhang
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Liu
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001201:Ascites / Q000378:metabolism; D001426:Bacterial Proteins; D006801:Humans; D007371:Interferon-gamma / Q000134:cerebrospinal fluid / Q000378:metabolism*; D007963:Leukocytes, Mononuclear; D009169:Mycobacterium tuberculosis; D010455:Peptides; D010996:Pleural Effusion / Q000276:immunology*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010455:Peptides; D011994:Recombinant Proteins; C072681:Mycobacterium tuberculosis antigens; D007371:Interferon-gamma