Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene.
Ethanol was a major byproduct of 2,3-butanediol (2,3-BD) fermentation by Klebsiella oxytoca ME-UD-3. In order to achieve a high efficiency of 2,3-BD production, K. oxytoca mutants deficient in ethanol formation were successfully constructed by replace the aldA gene coding for aldehyde dehydrogenase with a tetracycline resistance cassette. The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production. The carbon flux to 2,3-BD was enhanced by eliminating the byproducing ethanol and at the same time reducing the accumulation of another byproduct acetoin. At last, by fed-batch culturing of the mutant, the final 2,3-BD titer up to 130 g/l with the productivity of 1.63 g/l.h and the 2,3-BD yield relative to glucose of 0.48 g/g was obtained.
Ji
Xiao-Jun
XJ
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
Huang
He
H
Zhu
Jian-Guo
JG
Ren
Lu-Jing
LJ
Nie
Zhi-Kui
ZK
Du
Jun
J
Li
Shuang
S
D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D055785:Gene Knockdown Techniques; D005818:Genetic Engineering; D041121:Klebsiella oxytoca / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D001426:Bacterial Proteins; D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+)