title: Lipidomics of the red cell in diagnosis of human disorders.
abstract: Applications of tandem mass spectrometry in the field of lipid clinical chemistry are considered. Haemato-logical and biochemical advantages are presented favoring the choice of red blood cell membranes as a starting material in a wide variety of biomedical fields. Practical considerations are discussed with respect to methods of sampling, storage, and lipid extraction of red blood cells. The chapter describes the capabilities of a direct infusion of raw lipid extracts in the electro-spray ionization source compared with the more sophisticated method of high-performance liquid chromatography coupled with hybrid tandem mass spectrometry. Both methods have been evaluated and have been shown to be suitable for diagnosis and/or monitoring for a variety of human disorders.
authors.affiliation: Life Sciences, King's College, London, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Quinn
authors.forename: D
authors.initials: D
authors.lastname: Rainteau
authors.forename: C
authors.initials: C
authors.lastname: Wolf
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003933:Diagnosis; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006410:Hematopoiesis; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006801:Humans; D008563:Membrane Lipids / Q000097:blood*; D053719:Tandem Mass Spectrometry
chemical_list: D008563:Membrane Lipids