title: Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A.
abstract: Surface-activated chemical ionization (SACI) has been widely used in recent years for the analysis of different compounds (e.g. peptides, street drugs, amino acids). The main benefits of this technology are its high sensitivity and its effectiveness under different chromatographic conditions [i.e. ion exchange chromatography and reversed-phase (RP) chromatography]. Here we used SACI in conjunction with quadrupole time-of-flight mass spectrometry to analyze enterotoxin A, which is produced by Staphylococcus aureus, in milk matrix using both RP and ion exchange chromatographies. SACI had increased sensitivity as compared with electrospray ionization. Moreover, the higher quantitation efficiency of this technique, mainly in terms of limit of detection (0.01 ng/ml), limit of quantitation (0.05 ng/ml), linearity range (0.05-50 ng/ml), matrix effect, accuracy (intraday and interday accuracy errors were 9.2% and 10.3%, respectively) and precision (intraday and interday precision errors were 5.3% and 12.8%, respectively), is shown and discussed.
authors.affiliation: ISB-Ion Source & Biotechnologies, Milan, Italy. simone.cristoni@isbiotechnology.com
authors.forename: Simone
authors.initials: S
authors.lastname: Cristoni
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Zingaro
authors.forename: Cristina
authors.initials: C
authors.lastname: Canton
authors.forename: Paola
authors.initials: P
authors.lastname: Cremonesi
authors.forename: Bianca
authors.initials: B
authors.lastname: Castiglioni
authors.forename: Stefano
authors.initials: S
authors.lastname: Morandi
authors.forename: Milena
authors.initials: M
authors.lastname: Brasca
authors.forename: Massimo
authors.initials: M
authors.lastname: Luzzana
authors.forename: Cristina
authors.initials: C
authors.lastname: Battaglia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D004269:DNA, Bacterial / Q000032:analysis; D004768:Enterotoxins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D008892:Milk / Q000737:chemistry; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011817:Rabbits; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal