title: Complex formation of quercetin with lanthanum enhances binding to plant viral satellite double stranded RNA.
abstract: Due to the broad spectrum of biological activities of flavonoids, their target molecules in the cell are intensively studied. We examined the interactions of the flavonoid quercetin (Q) and its lanthanum complex (QLa(3+)) with very recently isolated plant viral satellite (sat) dsRNA. Comparison of the cumulative binding affinity and the estimated intercalative binding constant pointed towards an additional binding mode of quercetin to exclusively viral dsRNA, which is not recorded for synthetic dsRNAs. The QLa(3+) showed significantly higher affinity toward viral dsRNA than Q and La(3+) alone, most likely as the consequence of quercetin intercalation accompanied by additional electrostatic interaction of La(3+) with the negatively charged viral RNA backbone.
authors.affiliation: Department of Biology, Faculty of Science, University of Zagreb, 10 000 Zagreb, Croatia. gordana@botanic.hr
authors.forename: Gordana
authors.initials: G
authors.lastname: Rusak
authors.forename: Ivo
authors.initials: I
authors.lastname: Piantanida
authors.forename: Sabine
authors.initials: S
authors.lastname: Bretschneider
authors.forename: Jutta
authors.initials: J
authors.lastname: Ludwig-Müller
mesh_terms: D019401:Cucumber Mosaic Virus Satellite / Q000378:metabolism; D007811:Lanthanum / Q000378:metabolism*; D011794:Quercetin / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism*; D019244:RNA, Satellite / Q000378:metabolism*
chemical_list: D019401:Cucumber Mosaic Virus Satellite; D012330:RNA, Double-Stranded; D019244:RNA, Satellite; D007811:Lanthanum; D011794:Quercetin