title: Analysis of the mouse high-growth region in pigs.
abstract: In the mouse, homozygous animals for the high growth mutation show a 30-50% increase in growth without becoming obese. This region is homologous to the distal part of pig chromosome 5 (SSC5). A previous genome scan detected several quantitative trait loci (QTL) in this region for body composition and meat quality using a three generation Berkshire x Yorkshire resource family. In this study, the effects on swine growth, fat and meat quality traits of three genes previously identified within the mouse high growth region were analysed. The genes studied were CASP2 and RIPKI domain containing adaptor with death domain (CRADD), suppressor of cytokine signalling 2 (SOCS2) and plexinC1 (PLXNC1). In addition, the influence of two other genes located very close to this region, namely the plasma membrane calcium-transporting ATPase 1 (ATP2B1) and dual specificity phosphatase 6 (DUSP6) genes, was also investigated. Single nucleotide polymorphisms were identified and used to map these genes to the QTL region on SSC5. Results indicate significant associations between these genes and several phenotypic traits, including fat deposition and growth in pigs. The present study suggests associations of these genes with swine fat and growth related traits, but further studies are needed in order to clearly identify the genes involved in the regulation of the QTL located on SSC5.
authors.affiliation: Department of Animal Science, Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50010, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Ramos
authors.forename: R H
authors.initials: RH
authors.lastname: Pita
authors.forename: M
authors.initials: M
authors.lastname: Malek
authors.forename: P S
authors.initials: PS
authors.lastname: Lopes
authors.forename: S E F
authors.initials: SE
authors.lastname: Guimarães
authors.forename: M F
authors.initials: MF
authors.lastname: Rothschild
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D053420:CRADD Signaling Adaptor Protein / Q000235:genetics; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005260:Female; D016678:Genome / Q000235:genetics; D008297:Male; D008460:Meat; D051379:Mice / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics; D010641:Phenotype; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D011956:Receptors, Cell Surface / Q000235:genetics; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D053420:CRADD Signaling Adaptor Protein; C484481:Cradd protein, mouse; D009419:Nerve Tissue Proteins; C492112:Plxna3 protein, mouse; D011956:Receptors, Cell Surface; C491409:Socs2 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins