Promotion of triplex formation by 3'-amino-2'-O,4'-C-methylene bridged nucleic acid modification.
We examined the effect of 3'-amino-2'-O,4'-C-methylene bridged nucleic acid (3'-amino-2',4'-BNA) backbone modification of triplex-forming oligonucleotide (TFO) on the pyrimidine motif triplex formation at neutral pH, a condition where pyrimidine motif triplexes are unstable. The melting temperature of the pyrimidine motif triplex at pH 6.8 with 3'-amino-2',4'-BNA modified TFO was significantly higher than that observed with unmodified TFO. The 3'-amino-2',4'-BNA modification of TFO increased the thermal stability of the pyrimidine motif triplex at neutral pH. The present results certainly support the idea that the 3'-amino-2',4'-BNA modification of TFO could be a key chemical modification and may eventually lead to progress in therapeutic applications of the antigene strategy in vivo.
Sasaki
Kiyomi
K
Department of Applied Chemistry, Faculty of Sciecne, Tokyo University of Science, Tokyo 162-8601, Japan.
Rahman
S M Abdur
SM
Sato
Norihiro
N
Obika
Satoshi
S
Imanishi
Takeshi
T
Torigoe
Hidetaka
H
D001952:Bridged-Ring Compounds / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry; D011742:Pyrimidine Nucleotides / Q000737:chemistry*
D001952:Bridged-Ring Compounds; D009841:Oligonucleotides; D011742:Pyrimidine Nucleotides; C051802:triplex DNA; D004247:DNA