Lipidomics of the red cell in diagnosis of human disorders.
Applications of tandem mass spectrometry in the field of lipid clinical chemistry are considered. Haemato-logical and biochemical advantages are presented favoring the choice of red blood cell membranes as a starting material in a wide variety of biomedical fields. Practical considerations are discussed with respect to methods of sampling, storage, and lipid extraction of red blood cells. The chapter describes the capabilities of a direct infusion of raw lipid extracts in the electro-spray ionization source compared with the more sophisticated method of high-performance liquid chromatography coupled with hybrid tandem mass spectrometry. Both methods have been evaluated and have been shown to be suitable for diagnosis and/or monitoring for a variety of human disorders.
Quinn
Peter J
PJ
Life Sciences, King's College, London, UK.
Rainteau
D
D
Wolf
C
C
D002851:Chromatography, High Pressure Liquid; D003933:Diagnosis; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006410:Hematopoiesis; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006801:Humans; D008563:Membrane Lipids / Q000097:blood*; D053719:Tandem Mass Spectrometry
D008563:Membrane Lipids