Multilayer fluorescence optically encoded beads for protein detection.
An easy preparation method of multilayer fluorescence optically encoded beads for protein detection is presented. The beads, which consist of multicolored layers, are made from amino polyethylene glycol grafted polystyrene (PS-g-PEG) beads by using several fluorescent dyes such as fluorescein isothiocyanate (FITC) and rhodamine via controlling diffusion of an Fmoc-protecting group after HCl solution swelling. A biotin, glutathione S-transferase (GST) antibody, and an RNA aptamer that specifically recognize streptavidin, GST antigen, and hepatitis C virus (HCV) helicase are introduced to the optically encoded beads and monitored for their binding activity to the target molecules. After binding, the ligands are identified easily by their color codes.
Jun
Bong-Hyun
BH
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
Rho
Chul
C
Byun
Jang-Woong
JW
Kim
Jong-Ho
JH
Chung
Woo-Jae
WJ
Kang
Homan
H
Park
Juyoung
J
Cho
Su-Hyung
SH
Kim
Byung-Gee
BG
Lee
Yoon-Sik
YS
D052157:Aptamers, Nucleotide / Q000737:chemistry; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D020365:RNA Helicases / Q000032:analysis; D012235:Rhodamines / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011137:Polystyrenes; D011506:Proteins; D012235:Rhodamines; C103390:polyethylene glycol-polystyrene; D011092:Polyethylene Glycols; D001710:Biotin; D019809:Streptavidin; D005982:Glutathione Transferase; D020365:RNA Helicases; D016650:Fluorescein-5-isothiocyanate