title: [Influences of the phosphorylation of p38 mitogen activated protein kinase on gene expression of tumor necrosis factor-alpha in multiple organ dysfunction syndrome in pigs].
abstract: OBJECTIVE
  To investigate that the phosphorylation of the p38 mitogen activated protein kinase (p38MAPK) influences gene expression of tumor necrosis factor-alpha (TNF-alpha) in multiple organ dysfunction syndrome (MODS) in pigs.
  
  
  METHODS
  Thirty pigs were divided into MODS group and control group, and an animal model of MODS of "two-hit" injury, including hemorrhagic shock and endotoxemia, was reproduced. The content of p38MAPK's phosphorylation was assessed with Western blotting. TNF-alpha mRNA in peripheral blood monocytes was assayed with real time-polymerase chain reaction (RT-PCR). TNF-alpha was monitored in the peripheral blood plasma with enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Phosphorylation of p38MAPK was obviously increased in extent, which enhanced gene expression of TNF-alpha and then secretion of TNF-alpha by the peripheral blood mononuclear cell in MODS, and the differences were statistically significant compared with that of control group (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  p38MAPK's phosphorylation is important in pathogenesis of MODS, and phosphorylation of p38MAPK can enhance TNF-alpha mRNA transcription and secretion of TNF-alpha from peripheral blood mononuclear cells, which is the mechanism of increased TNF-alpha in MODS.
authors.affiliation: The PLA 85th Hospital, Shanghai, China.
authors.forename: An-rong
authors.initials: AR
authors.lastname: Mao
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Guo-en
authors.initials: GE
authors.lastname: Fang
authors.forename: Li-ye
authors.initials: LY
authors.lastname: Ma
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Bi
authors.forename: Tian-hang
authors.initials: TH
authors.lastname: Luo
authors.forename: Wen-zheng
authors.initials: WZ
authors.lastname: Fu
authors.forename: Ji-de
authors.initials: JD
authors.lastname: Hua
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D008297:Male; D009102:Multiple Organ Failure / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D048051:p38 Mitogen-Activated Protein Kinases