title: Topical administration of cyclosporin A in a solid lipid nanoparticle formulation.
abstract: Cyclosporin A (CsA)-loaded solid lipid nanoparticles (SLN) were developed for improved skin penetration. CsA-loaded SLN, prepared using a hot homogenization method, were nano-sized (about 73 nm) with a slightly negative surface charge (about -16 mV) and stable under physiological conditions regardless of CsA incorporation. In vitro permeation studies using murine skin mounted in the Franz-type vertical diffusion assembly revealed that the skin permeation efficiency of CsA-loaded SLN was 2-fold higher than that of CsA-oil mixture in viable skin. Furthermore, topically administered CsA-loaded SLN relieved symptoms of atopic dermatitis (AD) in an in vivo murine model of AD by decreasing the T helper (Th) 2 cell-related cytokines interleukin (IL)-4 and -5. These results suggest that SLN are effective drug carriers for topical delivery andthat CsA-loaded SLN can be therapeutically applied in allergy-related skin disorders.
authors.affiliation: Department of Biochemistry, Division of Brain Korea, College of Medicine, Korea University, Seoul, Korea. sungtae7@snu.ac.kr
authors.forename: Sung Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Jang
authors.forename: Jong Hyo
authors.initials: JH
authors.lastname: Kim
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Ji Soo
authors.initials: JS
authors.lastname: Lim
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Lee
authors.forename: Soo-Jeong
authors.initials: SJ
authors.lastname: Lim
authors.forename: Chong-Kook
authors.initials: CK
authors.lastname: Kim
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D016572:Cyclosporine / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D003876:Dermatitis, Atopic / Q000188:drug therapy; D004563:Electrochemistry; D005260:Female; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D008081:Liposomes; D051379:Mice; D008812:Mice, Hairless; D053758:Nanoparticles; D010316:Particle Size; D012869:Skin Absorption
chemical_list: D007166:Immunosuppressive Agents; D008081:Liposomes; D016572:Cyclosporine