Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis.
A prospective study was designed to evaluate the clinical usefulness of spoligotyping applied directly to sputum samples. Patients suspected of having tuberculosis were recruited at the Hospital Sanatorio Partenon in Porto Alegre, Brazil. Of the 197 samples included in the analysis, 175 (88.8%) yielded a spoligotyping result that fully matched that obtained from culture. Low bacillary samples presented lower accuracy (50%). From 135 Mycobacterium tuberculosis spoligopatterns, we identified 44 different spoligotypes, of which 21 were shared patterns and 23 were unique. T1 was the most frequent subfamily. The genotyping strategy proposed here presents a short turnaround time and could be helpful in providing rapid information on strain identities in a clinical setting.
Cafrune
Patricia Izquierdo
PI
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600, Porto Alegre, RS 90035-003, Brazil. patricia_cafrune@hotmail.com
Possuelo
Lia Gonçalves
LG
Ribeiro
Andrezza Wolowski
AW
Ribeiro
Marta Osório
MO
Unis
Gisela
G
Jarczewski
Carla Adriane
CA
Rossetti
Maria Lúcia Rosa
ML
Zaha
Arnaldo
A
D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D011446:Prospective Studies; D013183:Sputum / Q000737:chemistry / Q000382:microbiology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D004269:DNA, Bacterial