Hyaluronic acid and chitosan-DNA complex multilayered thin film as surface-mediated nonviral gene delivery system.
Sustained release of DNA from the surface of materials represents a promising approach to combine the gene therapy and implantable biomaterials. The nonviral chitosan-DNA complexes were incorporated into the multilayer via layer-by-layer deposition with hyaluronic acid (HA). The UV-vis spectroscopy and atomic force microscopy (AFM) results showed the successful construction of the nonviral complex contained multilayers. The complexes were releasable in physiological condition and a sustained release manner was gained when the multilayer was crosslinked. The cell viability test and the gene transfection assay showed that the natural polyelectrolyte-based nonviral complex incorporated multilayer not only had good cytocompatibility, but also possessed the in vitro gene transfection ability. This kind of surface-mediated nonviral complex incorporated multilayer may have great potential in the localized and controlled delivery of DNA in biomedical implants and tissue engineering application.
Lin
Quan-Kui
QK
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
Ren
Ke-Feng
KF
Ji
Jian
J
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D018014:Gene Transfer Techniques; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties / Q000187:drug effects; D014162:Transfection; D014780:Viruses / Q000235:genetics
D003432:Cross-Linking Reagents; D006820:Hyaluronic Acid; D004247:DNA; D048271:Chitosan