Threading the needle: getting selenocysteine into proteins.
The co-translational incorporation of selenocysteine (Sec) requires that UGA be recognized as a sense rather than a nonsense codon. This is accomplished by the concerted action of a Sec insertion sequence (SECIS) element, SECIS binding protein 2, and a ternary complex of the Sec specific elongation factor, Sec-tRNA(Sec), and GTP. The mechanism by which they alter the canonical protein synthesis reaction has been elusive. Here we present an overview of the mechanistic perspective on Sec incorporation, highlighting recent advances in the field.
Donovan
Jesse
J
Department of Microbiology, Molecular Genetics, and Immunology, Graduate School of Biomedical Sciences, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, NJ 08854, USA.
Copeland
Paul R
PR
D000818:Animals; D003062:Codon; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020871:RNA Stability; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D017279:Selenocysteine / Q000378:metabolism*
D003062:Codon; D020033:Protein Isoforms; D011506:Proteins; D012346:RNA, Transfer, Amino Acyl; D016601:RNA-Binding Proteins; C119984:selenocysteine insertion sequence binding protein, mammalian; C112705:selenocysteinyl-tRNA; D017279:Selenocysteine; D012313:RNA; D006160:Guanosine Triphosphate