A PCR-based SNP marker for specific authentication of Korean ginseng (panax ginseng) cultivar "Chunpoong".
Korean ginseng (Panax ginseng) has been developed as a horticultural crop due to the increasing demand in the world market. "Chunpoong" is an economically important cultivar with superior quality and high yield among nine cultivars of Korean ginseng. The aim of this work was to develop a simple technique for specific authentication of Chunpoong using DNA method. Molecular authentication of Chunpoong was investigated using DNA sequences of mitochondrial cytochrome oxidase subunit 2 (cox2) intron I and intron II regions. A single nucleotide polymorphism (SNP) specific to Chunpoong was detected and amplification refractory mutation system (ARMS)-PCR method was applied to specific identification of Chunpoong based on the SNP site. Ginseng samples collected from other locations were used to validate the SNP marker and the established method was determined to be effective. Thus, this work provides a rapid and reliable method for the specific identification of Chunpoong cultivar.
Wang
Hongtao
H
Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, Gyeonggi-do, 446-701, Republic of Korea.
Sun
Hua
H
Kwon
Woo-Saeng
WS
Jin
Haizhu
H
Yang
Deok-Chun
DC
D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods; D018744:DNA, Plant / Q000032:analysis; D005819:Genetic Markers; D008969:Molecular Sequence Data; D005894:Panax / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers