COP1 contributes to UVB-induced signaling in human keratinocytes.
UVB irradiation has been shown to trigger a broad range of changes in gene expression in human skin; however, factors governing these events are still not well understood. In this study, we show that human constitutive photomorphogenic protein-1 (huCOP1), an E3 ligase, contributes to the orchestration of UVB response of keratinocytes. Accordingly, our data show that (i) huCOP1 protein is expressed both in the nucleus and in the cytoplasm of cultured keratinocytes, (ii) UVB reduces the levels of the huCOP1 mRNA and protein, and (iii) induces changes in the subcellular localization of huCOP1. Finally, we show that gene-specific silencing of huCOP1 induces the accumulation of the tumor suppressor p53 protein, which is further increased after UVB irradiation.
Kinyó
Agnes
A
Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary. kinyoagnes@freemail.hu
Kiss-László
Zsuzsanna
Z
Hambalkó
Szabolcs
S
Bebes
Attila
A
Kiss
Mária
M
Széll
Márta
M
Bata-Csörgo
Zsuzsanna
Z
Nagy
Ferenc
F
Kemény
Lajos
L
D001706:Biopsy; D002467:Cell Nucleus / Q000378:metabolism; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014466:Ultraviolet Rays
D012333:RNA, Messenger; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C471981:COP1 protein, human; D044767:Ubiquitin-Protein Ligases