Quadrupole-central-transition 17O NMR spectroscopy of protein-ligand complexes in solution.
We report the first (17)O quadrupole-central-transition (QCT) NMR spectroscopic study of protein-ligand complexes in solution. This work shows that it is possible to obtain high resolution (17)O NMR spectra for (17)O-labeled ligands bound to proteins. At high magnetic fields such as 21.14 T, this (17)O QCT NMR approach should be applicable to studies of all oxygen-containing functional groups in large proteins (>30 kDa).
Zhu
Jianfeng
J
Department of Chemistry, Queen's University, 90 Bader Lane, Kingston, Ontario, Canada K7L 3N6.
Kwan
Irene C M
IC
Wu
Gang
G
D006801:Humans; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D019815:Oxalic Acid / Q000737:chemistry; D010103:Oxygen Isotopes; D019308:Palmitic Acid / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions
D008024:Ligands; D010103:Oxygen Isotopes; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D019308:Palmitic Acid; D019815:Oxalic Acid