title: Characterization of spliced leader genes of Trypanosoma ( Megatrypanum) theileri: phylogeographical analysis of Brazilian isolates from cattle supports spatial clustering of genotypes and parity with ribosomal markers.
abstract: Trypanosoma (Megatrypanum) theileri from cattle and trypanosomes of other artiodactyls form a clade of closely related species in analyses using ribosomal sequences. Analysis of polymorphic sequences of a larger number of trypanosomes from broader geographical origins is required to evaluate the clustering of isolates as suggested by previous studies. Here, we determined the sequences of the spliced leader (SL) genes of 21 isolates from cattle and 2 from water buffalo from distant regions of Brazil. Analysis of SL gene repeats revealed that the 5S rRNA gene is inserted within the intergenic region. Phylogeographical patterns inferred using SL sequences showed at least 5 major genotypes of T. theileri distributed in 2 strongly divergent lineages. Lineage TthI comprises genotypes IA and IB from buffalo and cattle, respectively, from the Southeast and Central regions, whereas genotype IC is restricted to cattle from the Southern region. Lineage TthII includes cattle genotypes IIA, which is restricted to the North and Northeast, and IIB, found in the Centre, West, North and Northeast. PCR-RFLP of SL genes revealed valuable markers for genotyping T. theileri. The results of this study emphasize the genetic complexity and corroborate the geographical structuring of T. theileri genotypes found in cattle.
authors.affiliation: Departamento de Parasitologia, Universidade de São Paulo, São Paulo, SP, 05508-900, Brasil.
authors.forename: A C
authors.initials: AC
authors.lastname: Rodrigues
authors.forename: H A
authors.initials: HA
authors.lastname: Garcia
authors.forename: J S
authors.initials: JS
authors.lastname: Batista
authors.forename: A H H
authors.initials: AH
authors.lastname: Minervino
authors.forename: G
authors.initials: G
authors.lastname: Góes-Cavalcante
authors.forename: F
authors.initials: F
authors.lastname: Maia da Silva
authors.forename: R C
authors.initials: RC
authors.lastname: Ferreira
authors.forename: M
authors.initials: M
authors.lastname: Campaner
authors.forename: F
authors.initials: F
authors.lastname: Paiva
authors.forename: M M G
authors.initials: MM
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002020:Buffaloes / Q000469:parasitology; D002417:Cattle / Q000469:parasitology*; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D019143:Evolution, Molecular; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D020038:RNA, Spliced Leader / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D020038:RNA, Spliced Leader