Osmium complex binding to mismatched methylcytosine: effect of adjacent bases.
We investigated the efficiency of osmium complex formation at 5-methylcytosine in mismatched DNA duplexes. Osmium complexation was not observed in fully matched duplexes, whereas the complexation site and efficiency in mismatched duplexes depended on the 5'-neighboring base of the 5-methylcytosine. In particular, when the base adjacent to the 5' side of the mismatched base pair was thymine, a unique side reaction was observed. However, the mismatched base pairs did not influence the selectivity of osmium complexation with methylated DNA.
Nomura
Akiko
A
RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
Tainaka
Kazuki
K
Okamoto
Akimitsu
A
D044503:5-Methylcytosine / Q000737:chemistry*; D020137:Base Pair Mismatch; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction; D013941:Thymine / Q000737:chemistry
D009992:Osmium; D044503:5-Methylcytosine; D013941:Thymine