[The relationship between PTEN expression and PI3K/AKT signal pathway in colon carcinoma].
OBJECTIVE
To investigate the expression of PTEN in carcinogenesis and development of colon carcinoma and to reveal their correlation with the PI3K/AKT pathway.


METHODS
The expressions of PTEN and p-AKT were detected by immunohistochemistry and western blotting in 32 specimens from patients with colon carcinoma.


RESULTS
The positive rate of p-PTEN was significantly lower in colon carcinoma (31.25%) than that in normal control tissues (96.87%, P<0.01), and the positive rate of p-AKT was significantly higher in colon carcinoma (87.5%) than that in normal control tissues (15.62%, P<0.01), and expression level of PTEN and p-AKT were related to histological differentiation, distant invasion, lymph node metastasis and Duke's stage (P<0.05). A negative correlation was observed between the expression of PTEN and p-AKT proteins. (r=-0.872, P<0.01).


CONCLUSIONS
The over-expression of AKT accompanied by the loss of PTEN involves carcinogenesis and development of colon carcinoma.
Qi
Jian-Kang
JK
Department of Internal Medicine, West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Huang
Xiao-Li
XL
Meng
Zhang-Ming
ZM
Gan
Hua-Tian
HT
Fu
Dai-Gang
DG
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction
D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human