title: Capillary electrophoresis with dual laser detection in separation of amplified fragment length polymorphism fragments.
abstract: We are presenting the application of CE technique with dual-channel LIF detection for the simultaneous separation of DNA fragments labeled with two different fluorescence dyes. The optimal conditions of the analysis were determined for the separation of amplified fragment length polymorphism (AFLP) fragments labeled with 5'-6-carboxyfluorescein (6-FAM) and the DNA size standard labeled with sulfoindocyanine succinimidyl ester (Cy-5). CE equipped with both argon ion and diode lasers is a good alternative for sequencers and might be applied in analyses of PCR products generated by various fingerprinting methods.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland. justyna@biology.pl
authors.forename: Justyna
authors.initials: J
authors.lastname: Stefanowicz-Hajduk
authors.forename: Natalia
authors.initials: N
authors.lastname: Filipowicz
authors.forename: Igor
authors.initials: I
authors.lastname: Kosinski
authors.forename: J Renata
authors.initials: JR
authors.lastname: Ochocka
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D018744:DNA, Plant / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007834:Lasers; D031384:Polygonatum / Q000235:genetics
chemical_list: D002232:Carbocyanines; D018744:DNA, Plant; D005452:Fluoresceins; D005456:Fluorescent Dyes; C085321:cyanine dye 5; C024098:6-carboxyfluorescein