Live cell multicolor imaging of lipid droplets with a new dye, LD540.
A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.
Spandl
Johanna
J
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
White
Daniel J
DJ
Peychl
Jan
J
Thiele
Christoph
C
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000737:chemistry*; D000818:Animals; D019556:COS Cells; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D003116:Color; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008113:Liver Neoplasms / Q000473:pathology; D008565:Membrane Proteins; D051379:Mice; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014162:Transfection; D014404:Tubulin / Q000378:metabolism
C464942:AUP1 protein, human; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D008055:Lipids; D008565:Membrane Proteins; D014404:Tubulin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins