title: Regenerative effects of basic fibroblast growth factor on extracellular matrix production in aged rat vocal folds.
abstract: OBJECTIVE
  We investigated acute changes in extracellular matrix (ECM) gene expression and histologic changes in the deposition of collagen and hyaluronan (hyaluronic acid; HA) after basic fibroblast growth factor (bFGF) treatment of the aged rat vocal fold.
  
  
  METHODS
  For the polymerase chain reaction (PCR) experiments, we divided ten 18-month-old Sprague-Dawley rats into two groups that received serial injections of sham (saline solution) or bFGF (2 ng/microL) and euthanized them 2 weeks after the initial injection to investigate acute changes in ECM gene expression. We treated a separate group of 5 animals unilaterally and sacrificed them 4 weeks after the initial injection to investigate histologic changes in the deposition of collagen and HA.
  
  
  RESULTS
  Real-time PCR revealed significantly up-regulated HA synthase (HAS)-2, HAS-3, matrix metalloproteinase (MMP)-2, and procollagen type I gene expression in the bFGF treatment group as compared to the sham treatment group. Histologic staining revealed significantly increased deposition of HA in the bFGF-treated vocal fold as compared to the sham-treated vocal fold. No differences in ECM collagen levels were observed between treatment sides.
  
  
  CONCLUSIONS
  Basic fibroblast growth factor induced the up-regulation of HAS-2, HAS-3, MMP-2, and procollagen type I. Histologically, aged vocal folds treated with bFGF revealed increased deposition of HA as compared to sham-treated vocal folds.
authors.affiliation: Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee 37232-4480, USA.
authors.forename: Tsunehisa
authors.initials: T
authors.lastname: Ohno
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Yoo
authors.forename: Erik R
authors.initials: ER
authors.lastname: Swanson
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.forename: Robert H
authors.initials: RH
authors.lastname: Ossoff
authors.forename: Bernard
authors.initials: B
authors.lastname: Rousseau
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism; D000076002:Hyaluronan Synthases; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D011347:Procollagen / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects*; D014827:Vocal Cords / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D024042:Collagen Type I; D011347:Procollagen; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D014453:Glucuronosyltransferase; C491097:Has2 protein, rat; C491355:Has3 protein, rat; D000076002:Hyaluronan Synthases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9