title: Preparations of hammerhead ribozymes for investigations of their cleavable sequences.
abstract: Recently, in hammerhead ribozymes, newly identified loop-loop interaction was found to be important for their activation. Therefore, we chemically synthesized a hammerhead ribozyme with this extra loop sequences and its mutant ribozymes, as well as their substrate RNA strands in order to clarify their cleavable sequences. After purification with an anion exchange column chromatography, we were able to obtain 44mer and 20mer RNA.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Sendai 980-8578, Japan.
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Tateoka
authors.forename: Ikumi
authors.initials: I
authors.lastname: Kawahara
authors.forename: Satomi
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Kaichiro
authors.initials: K
authors.lastname: Haruta
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kondo
authors.forename: Chojiro
authors.initials: C
authors.lastname: Kojima
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016337:RNA, Catalytic; C427204:hammerhead ribozyme; D012313:RNA