title: DNA cross-link generated by a novel modified DNA containing a formyl group.
abstract: We here report the synthesis of novel modified nucleic acid containing a formyl group and the evaluation of interstrand cross-linking between the modified DNA and the complementary strand. The synthesis of the formyl-containing oligodeoxynucleotide (ODN) was achieved by a post synthetic modification of corresponding ODN containing a 1,2-diol. The ODN containing the diol moiety was successfully synthesized by a standard phosphoramidite method, and was quantitatively converted to the desired formyl-containing ODN by sodium periodate oxidation. Hybridizaiton of the formyl ODN and the complementary ODN produced the interstrand cross-link between the formyl group and N(6)-exocyclic amino group of adenine.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Shibata
authors.forename: Chikara
authors.initials: C
authors.lastname: Dohno
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D000225:Adenine / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D005561:Formates / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D005561:Formates; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C030544:formic acid; D004247:DNA; D000225:Adenine