Role of the Yes and Csk tyrosine kinases in the development of a pathological state in the human retina.
Amplification and a cloning of fragments of genes of human retina tyrosine kinases, the nucleotide sequences of which feature a high homology to the gene families of the Yes and Csk tyrosine kinases, and a cloning of the complete coding sequence of the cDNA of the Csk tyrosine kinase gene of the human lymphocytes have been carried out. It has been established that this sequence contains 1,624 bp and encodes a protein that, with a 99% homology, corresponds to the human tyrosine kinase. A comparative analysis of the nucleotide sequences of the full-size cDNA of the Csk tyrosine kinase of the lymphocytes of healthy donors and of patients with an eye choroidal melanoma has shown that a risk of development of an eye choroidal melanoma can be estimated by the frequency of occurrence of a mutant allele in the 10th exon.
Baranova
Lyudmila
L
Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Akademicheskaya str., 27, 220072 Minsk, Belarus. r344@biobel.bas-net.by
Emelyanova
Valentina
V
Volotovski
Igor
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D000081247:CSK Tyrosine-Protein Kinase; D002830:Choroid Neoplasms / Q000201:enzymology / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005091:Exons / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005787:Gene Frequency / Q000235:genetics; D006262:Health; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D008545:Melanoma / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051575:Proto-Oncogene Proteins c-yes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012160:Retina / Q000201:enzymology* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014019:Tissue Donors; D019061:src-Family Kinases
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases; C000631346:CSK protein, human