Label-free photoelectrochemical immunoassay for alpha-fetoprotein detection based on TiO(2)/CdS hybrid.
A novel photoelectrochemical immunosensor based on TiO(2)/CdS hybrid modified electrode was developed. The TiO(2)/CdS hybrid modified electrode was obtained by alternately dipping the TiO(2) modified indium-tin oxide (ITO) electrode into the [Cd(NH(3))(4)](2+) and S(2-) solution repeatedly. Compared with the routine method using Cd(2+) solution for CdS deposition, the as obtained TiO(2)/CdS electrode showed enhanced photocurrent intensity with fewer coating times. After the ITO/TiO(2)/CdS electrode was coated with chitosan (CS), alpha-fetoprotein (AFP) antibodies were covalently conjugated on the surface of the electrode. Thus, a label-free photoelectrochemical immunosensor for the detection of AFP was developed by monitoring the changes in the photocurrent signals of the electrode resulting from the immunoreaction. The immunosensor displayed a linear response to AFP in the ranges from 50pg/mL to 50ng/mL with a relatively low detection limit of 40pg/ml. The photoelectrochemical results for the detection of AFP in five human sera showed acceptable accuracy. The method is simple, sensitive and specific. Moreover, the studied immunosensor possessed acceptable reproducibility and storage stability. The proposed methodology was potentially attractive for clinical immunoassay.
Wang
Guang-Li
GL
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Xu
Jing-Juan
JJ
Chen
Hong-Yuan
HY
Fu
Shou-Zhong
SZ
D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D010783:Photometry / Q000295:instrumentation*; D018036:Selenium Compounds / Q000737:chemistry*; D013194:Staining and Labeling; D014025:Titanium / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
D019187:Cadmium Compounds; D018036:Selenium Compounds; D000509:alpha-Fetoproteins; C009495:titanium dioxide; C058667:cadmium selenide; D014025:Titanium