Capillary electrophoresis with dual laser detection in separation of amplified fragment length polymorphism fragments.
We are presenting the application of CE technique with dual-channel LIF detection for the simultaneous separation of DNA fragments labeled with two different fluorescence dyes. The optimal conditions of the analysis were determined for the separation of amplified fragment length polymorphism (AFLP) fragments labeled with 5'-6-carboxyfluorescein (6-FAM) and the DNA size standard labeled with sulfoindocyanine succinimidyl ester (Cy-5). CE equipped with both argon ion and diode lasers is a good alternative for sequencers and might be applied in analyses of PCR products generated by various fingerprinting methods.
Stefanowicz-Hajduk
Justyna
J
Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland. justyna@biology.pl
Filipowicz
Natalia
N
Kosinski
Igor
I
Ochocka
J Renata
JR
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D018744:DNA, Plant / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007834:Lasers; D031384:Polygonatum / Q000235:genetics
D002232:Carbocyanines; D018744:DNA, Plant; D005452:Fluoresceins; D005456:Fluorescent Dyes; C085321:cyanine dye 5; C024098:6-carboxyfluorescein