Diagnostic value of enzyme-linked immunosorbent assays using hypothetical proteins CT226 and CT795 as antigens in Chlamydia trachomatis serodiagnosis.
The CT226 and the CT795 proteins were produced as purified recombinant proteins and were used as antigens in enzyme-linked immunosorbent assay (ELISA) tests for the detection of Chlamydia trachomatis IgG antibodies. The performances of the developed ELISA tests were compared with our in-house microimmunofluorescence test and the species-specific pELISA test using a panel of 342 sera. Our results indicate that the performance of the CT795 ELISA test was higher than that of the CT226 ELISA test according to the microimmunofluorescence and to the pELISA. To assess whether a combination of tests could improve the serodiagnosis of C. trachomatis infections, we associated results obtained with these tests to that using the previously developed CT694 ELISA test. Combining ELISA test results did not improve significantly the performances of these ELISA tests. The CT795 ELISA test, showing the highest performance, may be used for the serodiagnosis of C. trachomatis infections.
Frikha-Gargouri
Olfa
O
Department of Microbiology and Research Laboratory Microorganismes et Pathologie Humaine, Habib Bourguiba Hospital of Sfax, Tunisia.
Gdoura
Radhouane
R
Znazen
Abir
A
Gargouri
Jalel
J
Hammami
Adnene
A
D000942:Antigens, Bacterial / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D003627:Data Interpretation, Statistical; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D011994:Recombinant Proteins