title: Thermosporothrix hazakensis gen. nov., sp. nov., isolated from compost, description of Thermosporotrichaceae fam. nov. within the class Ktedonobacteria Cavaletti et al. 2007 and emended description of the class Ktedonobacteria.
abstract: We isolated from compost an aerobic, thermophilic, Gram-stain-positive, spore-forming bacterium that formed branched vegetative and aerial mycelia. This strain, designated SK20-1T, grew at 31-58 degrees C, with optimum growth at 50 degrees C, while no growth was observed below 28 or above 60 degrees C. The pH range for growth was 5.4-8.7, with optimum growth at pH 7.0, while no growth was observed below pH 5.0 or above pH 9.1. Strain SK20-1T was able to hydrolyse polysaccharides such as cellulose, xylan and chitin. The DNA G+C content was 54.0 mol%. The major fatty acid was iso-C17:0 and the major menaquinone was MK-9(H2). The cell wall contained glutamic acid, serine, alanine and ornithine in a molar ratio of 1.00:1.07:2.64:0.83. The polar lipids consisted of phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. Cell-wall sugars were rhamnose and mannose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK20-1T belongs to the class Ktedonobacteria, and that the strain is most closely related to Ktedonobacter racemifer SOSP1-21(T) (88.5 %). On the basis of its phenotypic features and phylogenetic position, we propose that SK20-1T represents a novel genus and species, Thermosporothrix hazakensis gen. nov., sp. nov., within the new family Thermosporotrichaceae fam. nov. The type strain of Thermosporothrix hazakensis is strain SK20-1(T) (=JCM 16142T =ATCC BAA-1881T). In addition, we propose an emended description of the class Ktedonobacteria to classify the class in the phylum Chloroflexi.
authors.affiliation: Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yabe
authors.affiliation: F.T. Innovation, 6F Royal Ikebukuro Bldg, 39-9, Nishi-Ikebukuro 2-chome Toshima-ku, Tokyo 171-0021, Japan.|Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Aiba
authors.affiliation: Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Sakai
authors.affiliation: Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Hazaka
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yokota
mesh_terms: D041862:Chloroflexi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S