Application of 4-iodophenol-enhanced luminol chemiluminescence to direct detection of horseradish peroxidase encapsulated in liposomes.
4-Iodophenol was applied to an enhancer in the direct detection of horseradish peroxidase (HRP) encapsulated in liposomes by using luminol chemiluminescence (CL). Luminol, 4-iodophenol and hydrogen peroxide permeate into the inner phase of liposomes containing HRP, resulting in the progress of 4-iodophenol-enhanced luminol CL catalyzed by HRP in liposomes. The CL intensity observed in liposomes was a factor of 150 greater than that observed in a lipid-free bulk solution. The detection limit in the direct detection of HRP encapsulated in liposomes was sensitive by a factor of 30 compared with that in a lipid-free bulk solution. 4-Iodophenol effectively functioned as an enhancer in HRP-catalyzed luminol CL in liposomes.
Kamidate
Tamio
T
Division of Biotechnology and Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo 060-8268, Japan.
Maruya
Masumi
M
Tani
Hirofumi
H
Ishida
Akihiko
A
D055162:Biocatalysis; D006735:Horseradish Peroxidase / Q000032:analysis* / Q000378:metabolism; D007462:Iodobenzenes / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000378:metabolism*
D007462:Iodobenzenes; D008081:Liposomes; C050181:4-iodophenol; D008165:Luminol; D006735:Horseradish Peroxidase