Development of a high-performance liquid chromatography-fluorescence detection method for analyzing nonylphenol/dinonylphenol-polyethoxylate-based phosphate esters.
A novel reversed phase HPLC method for the simultaneous analysis of surfactants containing nonylphenol/dinonylphenol-polyethoxylates and their o-phosphate esters is reported, in which eluting substances are detected fluorescently. Their chemical structures were elucidated by direct infusion electrospray-mass spectrometry in positive mode. The limits of quantification and range of the method were determined to be 0.1mg and 0.1-100 mg surfactant L(-1), respectively, with a reproducibility (RSD) at a concentration of 38 mg surfactant L(-1) of 5.6%. The accuracy was determined by spiking selected process water samples with known amounts of surfactant, and recoveries were typically in the 82-102% range.
Stenholm
Ake
A
Quality Control Support, GE Healthcare, Björkgatan 30, S-751 84 Uppsala, Sweden. ake.stenholm@ge.com
Holmström
Sara
S
Hjärthag
Sandra
S
Lind
Ola
O
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters; D007220:Industrial Waste; D015394:Molecular Structure; D010755:Organophosphates / Q000032:analysis* / Q000737:chemistry; D010636:Phenols / Q000032:analysis; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry*
D004952:Esters; D007220:Industrial Waste; D010755:Organophosphates; D010636:Phenols; D013501:Surface-Active Agents; C025256:nonylphenol