title: TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
abstract: Inflammation is associated with blood vessel and lymphatic vessel proliferation and remodeling. The microvasculature of the mouse trachea provides an ideal opportunity to study this process, as Mycoplasma pulmonis infection of mouse airways induces widespread and sustained vessel remodeling, including enlargement of capillaries into venules and lymphangiogenesis. Although the mediators responsible for these vascular changes in mice have not been identified, VEGF-A is known not to be involved. Here, we sought to determine whether TNF-alpha drives the changes in blood vessels and lymphatics in M. pulmonis-infected mice. The endothelial cells, but not pericytes, of blood vessels, but not lymphatics, were immunoreactive for TNF receptor 1 (TNF-R1) and lymphotoxin B receptors. Most TNF-R2 immunoreactivity was on leukocytes. Infection resulted in a large and sustained increase in TNF-alpha expression, as measured by real-time quantitative RT-PCR, and smaller increases in lymphotoxins and TNF receptors that preceded vessel remodeling. Substantially less vessel remodeling and lymphangiogenesis occurred when TNF-alpha signaling was inhibited by a blocking antibody or was silenced in Tnfr1-/- mice. When administered after infection was established, the TNF-alpha-specific antibody slowed but did not reverse blood vessel remodeling and lymphangiogenesis. The action of TNF-alpha on blood vessels is probably mediated through direct effects on endothelial cells, but its effects on lymphangiogenesis may require inflammatory mediators from recruited leukocytes. We conclude that TNF-alpha is a strong candidate for a mediator that drives blood vessel remodeling and lymphangiogenesis in inflammation.
authors.affiliation: Cardiovascular Research Institute, Comprehensive Cancer Center, and Department of Anatomy, University of California-San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA. baluk@ucsf.edu
authors.forename: Peter
authors.initials: P
authors.lastname: Baluk
authors.forename: Li-Chin
authors.initials: LC
authors.lastname: Yao
authors.forename: Jennifer
authors.initials: J
authors.lastname: Feng
authors.forename: Talia
authors.initials: T
authors.lastname: Romano
authors.forename: Sonia S
authors.initials: SS
authors.lastname: Jung
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Schreiter
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: David J
authors.initials: DJ
authors.lastname: Shealy
authors.forename: Donald M
authors.initials: DM
authors.lastname: McDonald
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology / Q000502:physiology; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D007249:Inflammation / Q000276:immunology*; D042583:Lymphangiogenesis / Q000276:immunology*; D042601:Lymphatic Vessels / Q000033:anatomy & histology / Q000502:physiology; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009175:Mycoplasma Infections / Q000276:immunology / Q000473:pathology; D045803:Mycoplasma pulmonis; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics / Q000276:immunology; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000235:genetics / Q000276:immunology; D012137:Respiratory System / Q000033:anatomy & histology / Q000276:immunology; D015398:Signal Transduction / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D006023:Glycoproteins; D026901:Membrane Transport Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha; C492273:LYVE1 protein, mouse