Effect of retinoic acid on renal development in newborn mice treated with an angiogenesis inhibitor.
BACKGROUND
A mouse model of impaired renal development was developed and the effect of retinoic acid (RA) was investigated in this animal model.


METHODS
An angiogenesis inhibitor (SU1498) was injected s.c. into day 3 C57BL/6 newborn mice to create a model of arrested renal development. RA (2 mg/kg) was injected i.p. for 10 days. Morphometry and immunohistochemistry were done.


RESULTS
Mice injected with SU1498 demonstrated deranged renal development in tubular structure and glomerular tuft area. Cortical thickness and area of glomerular tuft were significantly decreased after vascular endothelial growth factor (VEGF) inhibitor, and were significantly restored by RA. The length of capillary loops/glomerulus, the number of podocytes/glomerulus, and density of peritubular capillaries on CD31 immunostaining were significantly decreased by VEGF blocking and recovered by RA.


CONCLUSIONS
VEGF plays a major role in renal development, and RA reverses the inhibited development caused by an angiogenesis inhibitor.
Lee
Soo Jeong
SJ
Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Republic of Korea.
Cho
Su Jin
SJ
Ju
Sun Young
SY
Ryu
Kyung-Ha
KH
Sung
Soon Hee
SH
Park
Eun Ae
EA
D000704:Analysis of Variance; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001707:Biopsy, Needle; D001835:Body Weight; D002934:Cinnamates / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects* / Q000254:growth & development*; D007672:Kidney Cortex / Q000187:drug effects / Q000254:growth & development; D007678:Kidney Glomerulus / Q000187:drug effects / Q000254:growth & development; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D018709:Statistics, Nonparametric; D014212:Tretinoin / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D002934:Cinnamates; C483044:SU 1498; D014212:Tretinoin