[Effect of phospholipid composition on characteristics of liposomes containing zedoary turmeric oil].
OBJECTIVE
To study the effect of phospholipids composition on pharmaceutical characteristics of liposomes containing zedoary turmeric oil (ZTO).


METHODS
The ZTO was extracted supersonically by solvent and then colored by sulfuric acid-vanillin reagent. The ZTO liposomes were prepared by ethanol injection method. To determine the entrapment efficiency of the ZTO liposomes, we passed the liposome suspension through a Sephadex G-50 column equilibrated with PBS. Pharmaceutical characteristics of different liposomes such as size, zata potential, entrapment efficiency, stablility were determined and compared.


RESULTS
The ZTO liposomes and free drug could be separated completely by the Sephadex G-50 chromatography, and the column recovery was (100.09+/-3.375)% (n=5). The ZTO liposomes composed of hydrogenated soybean phosphatidylcholine (HSPC) possessed the worst entrapment efficiency and sedimentation stability. The entrapment efficiency of the ZTO liposomes composed of soybean phosphatidylcholine (SPC) was equal to that of liposomes composed of both HSPC and SPC. However, The sedimentation and leakage stabilities of the latter were significantly higher than those of SPC liposomes.


CONCLUSIONS
As the carrier of the ZTO liposomes, the composition of both HSPC and SPC can improve its stability.
Chen
Jun
J
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. chenjun75@163.com
Zhu
Kai
K
Cheng
Na-Na
NN
Wang
Wei-Mei
WM
Bian
Zheng
Z
Lu
Tu-Lin
TL
Mao
Chun-Qin
CQ
Cai
Bao-Chang
BC
D000970:Antineoplastic Agents / Q000737:chemistry*; D030024:Curcuma / Q000737:chemistry; D004337:Drug Carriers; D006865:Hydrogenation; D008081:Liposomes; D009822:Oils, Volatile / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D012995:Solubility; D013025:Glycine max / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods
D000970:Antineoplastic Agents; D004337:Drug Carriers; D008081:Liposomes; D009822:Oils, Volatile; D010713:Phosphatidylcholines; D010743:Phospholipids