Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by DNA polymerases.
We describe herein the results of (i) enzymatic recognition for imidazopyridopyrimidine (Im):naphthyridine (Na) base pairs and (ii) further primer extension reactions after the Im:Na base pairs by DNA polymerases. Among the base pairs examined, ImN(O):NaO(N) base pair was rather selectively recognized by Klenow fragment exo(-) [F(exo(-))]as complementary base. However, this DNA polymerase did not catalyze primer extension reactions after the ImN(O):NaO(N) base pair. Therefore, we carried out a screening of DNA polymerases to promote the primer extension reaction as well as to improve the selectivity of base pair recognition. As a result, a family B DNA polymerase, especially Deep Vent (exo(-)), seemed most promising for this purpose.
Minakawa
Noriaki
N
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Ogata
Shintaro
S
Takahashi
Mayumi
M
Matsuda
Akira
A
D020029:Base Pairing; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D009287:Naphthyridines / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*
D009287:Naphthyridines; D011743:Pyrimidines; D004247:DNA; C088944:Deep Vent DNA polymerase; D004259:DNA-Directed DNA Polymerase