Multiple primer PCR for the identification of anisakid nematodes from Taiwan Strait.
There were six major larval anisakid species found in commercial marine fishes caught in the Minnan fishing ground in the Taiwan Strait: Anisakis physeteris, Anisakis pegreffii, Raphidascaris trichiuri, Contracaecum aduncum, Contracaecum muraenesoxi, Contracaecum sp. For rapid identification of the parasite species above, a single and a multiple primer PCR (multiplex PCR) method, using specific primers based on aligned sequences of the internal transcribed spacer ITS-1, 5.8S, and ITS-2 of nuclear ribosomal DNA, were jointly used for the rapid identification of these anisakid larvae. The primers yielded distinct PCR products for each of the anisakid nematodes, providing rapid and accurate tools for identifying anisakid nematodes with distinct geographical distribution.
Fang
Wenzhen
W
Biology Department, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
Xu
Shisan
S
Zhang
Shaolei
S
Wang
Yinan
Y
Chen
Xiaobin
X
Luo
Damin
D
D000818:Animals; D017191:Ascaridida Infections / Q000469:parasitology / Q000662:veterinary*; D001199:Ascaridoidea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012623:Seawater; D016415:Sequence Alignment; D013045:Species Specificity; D013624:Taiwan
D017931:DNA Primers; D018509:DNA, Helminth; D004275:DNA, Ribosomal