Metabonomic characterization of aging and investigation on the anti-aging effects of total flavones of Epimedium.
A liquid chromatography coupled with mass spectrometry (LC/MS) based metabonomics approach was applied to characterize the aging of rats, and the anti-aging effect of total flavones of Epimedium (TFE), a traditional Chinese medicine, has also been investigated. Serum samples collected from 4, 10, 18 and 24 month-old rats and TFE-administered rats have been profiled by LC/MS. Thirty age-related endogenous metabolites were discovered by partial least squares (PLS) and Hotelling's T(2) control chart, among which 25 metabolites were structurally identified by MS(n) analysis and ten of them were further confirmed via authentic chemicals. All important age-related metabolites, such as unsaturated fatty acids, saturated fatty acids, nucleotides, carnosine, ergothioneine and amino acids, displayed age-related changes, and most of them were reset to a younger level after TFE administration. This study indicated that aging could be characterized by changes of lipid metabolisms and accumulation of free radicals. The anti-aging effects of TFE might due to the intervention on lipid metabolism and its property of anti-oxidation.
Yan
Shikai
S
School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, PR China.
Wu
Bin
B
Lin
Zhongying
Z
Jin
Huizi
H
Huang
Jianhua
J
Yang
Yun
Y
Zhang
Xinmin
X
Shen
Ziyin
Z
Zhang
Weidong
W
D000375:Aging / Q000097:blood / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D034181:Epimedium / Q000737:chemistry*; D047309:Flavones / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000378:metabolism; D016018:Least-Squares Analysis; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D013058:Mass Spectrometry; D055432:Metabolomics; D015999:Multivariate Analysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
D047309:Flavones; D016166:Free Radical Scavengers; D010936:Plant Extracts; D008315:Malondialdehyde; D013482:Superoxide Dismutase