[Flavonoids from Diospyros kaki inhibit the adhesion between lymphocyte and dorsal root ganglion].
OBJECTIVE
To investigate the effects of flavonoids from the leaves of Diospyros kaki L (FLDK) on the adhesion between the lymphocyte and the neurone.


METHODS
Centrifugal assay for fluorescence-bsaed cell adhesion was used to assay the adhesion between the lymphocyte and the dorsal root ganglion (DRG).


RESULTS
The adhesion was significantly suppressed in the presence of FLDK dose-dependently at 5, 25 microg/mL concentration. FLDK was also effective to inhibit the adhesion under the challenge of ICAM-1 by 28.5% and 50.1%, respectively. Furthermore, FLDK enforced the inhibition of anti-NCAM antibody on the lymphocyte adhesion to DRG cells.


CONCLUSIONS
FLDK might contribute to the prevention and treatment of the inflammation injury under neuron insult such as ischemia/reperfusion, neurotrauma and other neurodegenerative disease by inhibiting the adhesion between lymphocytes and neurons.
Bei
Wei-Jian
WJ
Guangzhou Pharmaceutical University, The Institute of Chinese Medicinal Sciences, Guangzhou 510006, China. bei-wj2000@yahoo.com.cn
Xu
An-Long
AL
Li
Chu-Yuan
CY
Cabot
Peter J
PJ
Hermanussen
Siobhan
S
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D029788:Diospyros / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008297:Male; D019006:Neural Cell Adhesion Molecules / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D005419:Flavonoids; D019006:Neural Cell Adhesion Molecules; D020011:Protective Agents; D018799:Intercellular Adhesion Molecule-1