Role of neutrophils in arginine-asymmetric dimethylarginine pathway subsequent to endotoxemia.
There is a striking correlation between nitric oxide (NO) production and myeloperoxidase (MPO) enzyme activity accession in various tissues after endotoxemia. Both arginine-NO and arginine-asymmetric dimethylarginine (ADMA) pathways have been recognized to play critical roles during infection and inflammation. We investigated whether there is a link between MPO-mediated nitrating pathway and arginine-ADMA pathway after endotoxemia in liver. All experiments were performed in two groups (control and endotoxemia) of 10 guinea pigs. In this study, 6 h after the administration of endotoxin at a dose of 4 mg/kg, MPO activity and ADMA, L-arginine, and 3-nitrotyrosine (3-NT, a stable product of peroxynitrite formation) levels were measured. Measurement of ADMA and L-arginine were accomplished by HPLC with fluorescent detector. 3-NT was quantified by HPLC with electrochemical detector. MPO activity was determined by spectrophotometric method. After administration of endotoxin, ADMA and L-arginine levels decreased, but 3-NT levels and MPO activity increased significantly. In conclusion, there is an inverse relationship between MPO-mediated tyrosine nitration and arginine-ADMA pathway in liver after endotoxemia.
Nurten
Türközkan
T
Department of Biochemistry, Gazi University, Ankara, Turkey.
Nihal
Sahin
S
Gonca
Ozan
O
Ozgür
Akgül
A
Halil
Yaman
Y
Filiz
Bircan
B
Barbaros
Balabanli
B
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism*; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000139:chemically induced / Q000503:physiopathology*; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000633:toxicity; D008099:Liver / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D008070:Lipopolysaccharides; C002744:3-nitrotyrosine; D014443:Tyrosine; C018524:N,N-dimethylarginine; D001120:Arginine; D009195:Peroxidase