title: [The purification and characterization of a novel lipid transfer protein from caryopsis of barnyard grass (Echinochloa crusgalli)].
abstract: A novel lipid transfer protein called Ec-LTP was isolated from resting caryopsis of weed barnyard grass Echinochloa crusgalli (L.) Beauv.; its molecular weight, amino acid content and N-terminal amino acid sequence were determined. Ec-LTP was a 9150 Da protein, containing eight cysteine residues, which formed four disulfide bonds. The isolated protein could significantly inhibit the development of pathogenic fungi Phytophthora infestans and Helminthosporium sativum, causing the late blight of potato and tomato and the root rot of herbs, respectively.
authors.forename: E A
authors.initials: EA
authors.lastname: Rogozhin
authors.forename: T I
authors.initials: TI
authors.lastname: Odintsova
authors.forename: A Kh
authors.initials: AKh
authors.lastname: Musoliamov
authors.forename: A N
authors.initials: AN
authors.lastname: Smirnov
authors.forename: A V
authors.initials: AV
authors.lastname: Babakov
authors.forename: Ts A
authors.initials: TsA
authors.lastname: Egorov
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D031747:Echinochloa / Q000737:chemistry* / Q000235:genetics / Q000382:microbiology; D006375:Helminthosporium; D055750:Phytophthora infestans; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D020539:Sequence Analysis, Protein
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; C030363:lipid transfer protein