Analysis of myeloperoxidase promotor polymorphism and enzyme activity in Turkish patients with vitiligo.
Vitiligo is an acquired depigmentary disorder characterized by white areas of the skin due to loss of epidermal melanocytes. Oxidative stress and free radicals are suggested as important phenomena in the pathogenesis of vitiligo. Myeloperoxidase is a lysosomal enzyme of polymorphonuclear leukocytes and acts as a catalyst in the production of hypochlorous acid, a powerful oxidant. In this study we analysed enzyme activity and gene polymorphism of myeloperoxidase in patients with vitiligo. Fifty-four patients with vitiligo and 58 healthy controls were enrolled to this study. Patient groups were subdivided according to localization of the lesions; generalized, acrofacial and local. Plasma myeloperoxidase enzyme activity was determined with ELISA and G-463A gene (-463) polymorphism with the PCR-RFLP (AciI) method. The plasma MPO level was significantly lower in vitiligo patients than in the healthy controls (p = 0.005), however, it was not significantly different among subtypes of vitiligo (p = 0.8). A significant difference was not observed for G-463A genotype and allele distribution in patients with vitiligo. In conclusion, the present study is the first study investigating MPO G-463A polymorphism and enzyme levels, which warrants further studies with higher patient numbers and broader polymorphism panels.
Aksoy
S Nur
SN
Department of Biochemistry, Medical Faculty of Gaziantep University, 27310, Gaziantep, Turkey.
Erbagci
Zulal
Z
Saygili
E Ilker
EI
Sever
Tugce
T
Erbagci
A Binnur
AB
Pehlivan
Sacide
S
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005609:Free Radicals; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D009195:Peroxidase / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014421:Turkey; D014820:Vitiligo / Q000097:blood / Q000201:enzymology / Q000235:genetics* / Q000473:pathology
D005609:Free Radicals; D005819:Genetic Markers; D006997:Hypochlorous Acid; D009195:Peroxidase