Rapid SYBR green I and modified probe real-time reverse transcription-PCR assays identify influenza H1N1 viruses and distinguish between pandemic and seasonal strains.
A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid and simultaneous typing and subtyping of influenza viruses.
Yang
Ji-Rong
JR
National Influenza Center, Centers for Disease Control, No. 161, Kun-Yang Street, Taipei 11561, Taiwan, Republic of China.
Lo
Je
J
Liu
Jian-Liang
JL
Lin
Chao-Hua
CH
Ho
Yu-Lin
YL
Chen
Chun-Jung
CJ
Wu
Ho-Sheng
HS
Liu
Ming-Tsan
MT
D052160:Benzothiazoles; D003959:Diamines; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D015345:Oligonucleotide Probes / Q000235:genetics; D009930:Organic Chemicals / Q000378:metabolism; D011804:Quinolines; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics
D052160:Benzothiazoles; D003959:Diamines; D015345:Oligonucleotide Probes; D009930:Organic Chemicals; D011804:Quinolines; D014764:Viral Proteins; C098022:SYBR Green I