Human cytosolic tRNase ZL can downregulate gene expression through miRNA.
A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including approximately 25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3' untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.
Elbarbary
Reyad A
RA
Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Niigata 956-8603, Japan.
Takaku
Hiroaki
H
Uchiumi
Naoto
N
Tamiya
Hiroko
H
Abe
Mayumi
M
Nishida
Hiroshi
H
Nashimoto
Masayuki
M
D001483:Base Sequence; D045744:Cell Line, Tumor; D003600:Cytosol / Q000201:enzymology; D015536:Down-Regulation; D004722:Endoribonucleases / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009690:Nucleic Acid Conformation
C544749:MIRN103 microRNA, human; D035683:MicroRNAs; D004722:Endoribonucleases; C531011:tRNase Z, human