[Ribonuclease from the hepatopancreas of the red king crab Paralithodes camtschatica].
Three enzyme preparations, two acid and one alkaline RNases, were isolated from the hepatopancreas of the red king crab Paralithodes camtschatica using DEAE-cellulose chromatography and gel chromatography. The alkaline RNase was activated by Mg2+ ions and had a pH optimum of 7.2; the acid RNases, a pH optimum of 5.5. The molecular weight of the alkaline RNase was 19 kDa; two acid RNases, 33 and 70 kDa, respectively. The enzymes exhibited a sufficiently high thermostability (IT50 = 53-55 degrees C) and were strongly inhibited by NaCl (IC50, 0.1-0.25 M). The alkaline RNase exhibited no specificity for heterocyclic bases, whereas the acid RNases hydrolyzed poly(U) and poly(A) at maximum rates.
Menzorova
N I
NI
Sibirtsev
Iu T
IuT
Rasskazov
V A
VA
D000818:Animals; D033381:Anomura / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D043143:Hepatopancreas / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D012260:Ribonucleases / Q000737:chemistry* / Q000302:isolation & purification*
D012260:Ribonucleases