Involvement of SigT and RstA in the differentiation of Streptomyces coelicolor.
SigT is an ECF sigma factor in Streptomyces coelicolor. sigT and its putative anti-sigma factor gene rstA are located in one putative operon, and SigT could physically interact with RstA. Deletion of sigT or rstA caused accelerated morphological development and enhanced production of antibiotics, concomitant with over-expression of chpE, chpH, actII-orf4 and redD. Furthermore, SigT was undetectable after loss of rstA. These data suggested that SigT has a negative role on differentiation and that RstA negatively regulates the SigT activity through a putative antagonistic mechanism and at the post-transcriptional level.
Mao
Xu-Ming
XM
Zhejiang University, College of Life Sciences, Hangzhou 310058, China.
Zhou
Zhan
Z
Cheng
Liang-Ying
LY
Hou
Xiao-Ping
XP
Guan
Wen-Jun
WJ
Li
Yong-Quan
YQ
D002454:Cell Differentiation / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D012808:Sigma Factor / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D012808:Sigma Factor