title: Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex.
abstract: Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances in spectra affecting the quantification. As an alternative, thiopental can be administrated without any solubilizing agents. The aim of the study was to investigate the effect of deep thiopental anesthesia on the neurochemical profile consisting of 19 metabolites and on glucose transport kinetics in vivo in rat cortex compared with alpha-chloralose using localized (1)H NMR spectroscopy. Thiopental was devoid of effects on the neurochemical profile, except for the elevated glucose at a given plasma glucose level resulting from thiopental-induced depression of glucose consumption at isoelectrical condition. Over the entire range of plasma glucose levels, steady-state glucose concentrations were increased on average by 48% +/- 8%, implying that an effect of deep thiopental anesthesia on the transport rate relative to cerebral glucose consumption ratio was increased by 47% +/- 8% compared with light alpha-chloralose-anesthetized rats. We conclude that the thiopental-induced isoelectrical condition in rat cortex significantly affected glucose contents by depressing brain metabolism, which remained substantial at isoelectricity.
authors.affiliation: Laboratory of functional and metabolic imaging (LIFMET), Institute of the Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. hongxia.lei@epfl.ch
authors.forename: Hongxia
authors.initials: H
authors.lastname: Lei
authors.forename: Joao M N
authors.initials: JM
authors.lastname: Duarte
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mlynarik
authors.forename: Agathe
authors.initials: A
authors.lastname: Python
authors.forename: Rolf
authors.initials: R
authors.lastname: Gruetter
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D002698:Chloralose / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects*; D007526:Isoelectric Point; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011522:Protons; D051381:Rats; D017207:Rats, Sprague-Dawley; D013874:Thiopental / Q000494:pharmacology*
chemical_list: D018686:Anesthetics, Intravenous; D001786:Blood Glucose; D011522:Protons; D002698:Chloralose; D005947:Glucose; D013874:Thiopental