title: Transfection efficiencies of PAMAM dendrimers correlate inversely with their hydrophobicity.
abstract: Dendriplexes were characterized by ethidium bromide intercalation assay and their transfection efficiency was studied using HEK 293 cells and human mesenchymal stem cells. PAMAM G4 showed a higher transfection efficiency than PAMAM G3-G6, G4-OH, G4-25% or G4-50% dendrimers. Substitution of OH groups for the NH(2) surface groups rendered the dendrimer unable to form dendriplexes and to transfect cells. Partial (25%) substitution of CH(3) groups for the NH(2) groups markedly impaired transfection; 50% substitution decreased the ability of PAMAM G4 to transfect threefold. It was concluded that increased hydrophobicity decreased the ability of dendrimers to transfect. PAMAM G4-50% is highly hydrophobic and forms micelles in solution, which can transfect pGFP. The results of ethidium bromide intercalation assays, ANS fluorescence studies and transfection efficiencies of PAMAM dendrimers were correlated. Subsequently, we constructed a neurotrophin-encoding plasmid and studied its delivery to mesenchymal stem cells using PAMAM G4 dendrimer and Lipofectamine 2000. Lipofectamine 2000 was a more effective carrier (18.5%) than PAMAM G4 dendrimer (1.2%).
authors.affiliation: Institute of Genetics and Cytology of Natl Acad Sci Bel, Minsk, Belarus.
authors.forename: Antos
authors.initials: A
authors.lastname: Shakhbazau
authors.forename: Iauhenia
authors.initials: I
authors.lastname: Isayenka
authors.forename: Nikolai
authors.initials: N
authors.lastname: Kartel
authors.forename: Natalya
authors.initials: N
authors.lastname: Goncharova
authors.forename: Ihar
authors.initials: I
authors.lastname: Seviaryn
authors.forename: Svetlana
authors.initials: S
authors.lastname: Kosmacheva
authors.forename: Mihail
authors.initials: M
authors.lastname: Potapnev
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D002460:Cell Line; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013050:Spectrometry, Fluorescence; D014162:Transfection / Q000379:methods* / Q000639:trends
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst