Inhibition of influenza virus infection by targeting genome conserved region with non-natural nucleic acid.
Two highly conserved 15 base sequences of influenza A virus genome were identified by CONSERV software, which can detect contiguous conserved sequences of biological sequences. Antiviral effect of phosphorothioate oligonucleotide that target these conserved sequences was evaluated by plaque formation assay and cell viability assay. Pre-treatment of cells with anti-PB2 (RNA polymerase subunit) reduced the size of plaques in a sequence dependent manner. Post-treatment of cells with anti-PB2 phosphorothioate oligonucleotide inhibited the virus-induced cytotoxicity.
Takahashi
Tomoya
T
Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Ohzawa
Takuya
T
Sawada
Shinjiro
S
Kato
Nobuo
N
Goto
Naohisa
N
Nakamura
Shota
S
Yasunaga
Teruo
T
Kaihatsu
Kunihiro
K
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D004285:Dogs; D016679:Genome, Viral; D009980:Influenza A virus / Q000235:genetics* / Q000254:growth & development; D054735:Phosphorothioate Oligonucleotides / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors / Q000235:genetics; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics
C057027:PB2 protein, Influenzavirus A; D054735:Phosphorothioate Oligonucleotides; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase