Effects of thiopental on endothelium-dependent relaxation in porcine coronary arteries.
This study investigated the effects of thiopental on endothelium-dependent relaxation (EDR), and especially the effects on nitric oxide- and prostacyclin-independent EDR. Fresh porcine coronary artery rings (4 mm long), were consecutively tested with and without 20 microg/ml thiopental in Krebs-Henseleit solution. Indomethacin (10 micromol/l) was used in all experiments to eliminate prostacyclin effects. Prostaglandin F(2alpha) (10 micromol/l) was used to induce contractions and bradykinin (10(-10) - 10(-5) M) was used to induce EDR. Experiments were also carried out using 300 micromol/l N-nitro-L-arginine to block nitric oxide production and to assess the influence of thiopental on nitric oxide- and prostacyclin-independent EDR. Thiopental induced statistically significant increases in EDR at concentrations of 10(-6) - 10(-5) M bradykinin. Following nitric oxide production block, thiopental significantly reduced the relaxation response at concentrations of 10(-8) - 10(-5) M bradykinin. At a clinically relevant concentration of 20 microg/ml thiopental, a significant increase in EDR and a significant reduction in nitric oxide- and prostacyclin-independent relaxation was observed in porcine epicardial coronary arteries.
Dagtekin
O
O
Department of Anaesthesiology and Intensive Care Medicine, University of Cologne, Cologne, Germany. oguzhan.dagtekin@uk-koeln.de
Gerbershagen
H J
HJ
Ozgür
E
E
Gaertner
J
J
Fischer
J H
JH
D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001685:Biological Factors / Q000502:physiology; D001920:Bradykinin / Q000494:pharmacology; D003331:Coronary Vessels / Q000187:drug effects*; D015237:Dinoprost / Q000494:pharmacology; D004359:Drug Therapy, Combination; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D013552:Swine; D013874:Thiopental / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects*
D018686:Anesthetics, Intravenous; D001685:Biological Factors; C067410:endothelium-dependent hyperpolarization factor; D019335:Nitroarginine; D009569:Nitric Oxide; D015237:Dinoprost; D013874:Thiopental; D001920:Bradykinin