title: Expression of the mu opioid receptor and effects of the opioid antagonist naloxone on in vitro maturation of oocytes recovered from anoestrous bitches.
abstract: The mu-opioid receptor (MOR) is expressed in bovine, human, equine and canine oocytes, and in seasonal breeders, it is expressed with higher intensity during the anoestrous phase. Supplementation of in vitro maturation (IVM) medium with opioid agents, agonists or antagonists, was shown to affect oocyte maturation in several species such as rat, bovine and equine. This study reports the effects of supplementing IVM medium with naloxone (Nx), an opioid antagonist, on nuclear and cytoplasmic maturation rate of oocytes recovered from anoestrous bitches. Cytoplasmic maturation was examined in terms of mitochondrial (mt) distribution. In order to confirm the receptor-mediated action of Nx, in oocytes of anoestrous bitches, MOR expression was analyzed by Western blot. Cumulus-oocyte complexes, recovered from the ovaries of bitches in anoestrous, were cultured in vitro and Nx was added at the concentrations of 1 x 10(-6), 1 x 10(-8) and 1 x 10(-10) M. The rate of oocytes resuming meiosis after culture in presence of 1 x 10(-6) M Nx (29%) was significantly higher than that of oocytes of control group (12%; p < 0.05). However, treatment with Nx did not affect mt distribution pattern. In denuded oocytes and in corresponding cumulus cells, a doublet of 65 and 50 kDa was observed. We conclude that, in oocytes of anoestrous bitches, MOR is expressed and Nx significantly improves nuclear maturation rate. Further studies should be performed to elucidate the expression of other opioid receptors, such as delta and kappa, and possible interactive effects of their antagonists on canine oocyte maturation.
authors.affiliation: Department of Animal Production, University of Bari, Bari, Italy. i_alina@yahoo.com
authors.forename: A I
authors.initials: AI
authors.lastname: Iorga
authors.forename: L
authors.initials: L
authors.lastname: Valentini
authors.forename: T
authors.initials: T
authors.lastname: De Santis
authors.forename: B
authors.initials: B
authors.lastname: Ambruosi
authors.forename: M
authors.initials: M
authors.lastname: Albrizio
authors.forename: A C
authors.initials: AC
authors.lastname: Guaricci
authors.forename: M
authors.initials: M
authors.lastname: Caira
authors.forename: Me
authors.initials: M
authors.lastname: Dell'Aquila
mesh_terms: D000780:Anestrus / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D009270:Naloxone / Q000494:pharmacology*; D009292:Narcotic Antagonists / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000235:genetics / Q000378:metabolism*
chemical_list: D009292:Narcotic Antagonists; D017450:Receptors, Opioid, mu; D009270:Naloxone