title: Synthesis and characterization of deoxyuridine triphosphates labeled with pyrene.
abstract: Deoxyuridine triphosphate derivatives modified with pyrene was synthesized to functionalize DNA with fluorescent molecules based on the template DNA sequence. Incorporation of pyrene-labeled deoxyuridine triphosphates into DNA by DNA polymerase was investigated by using reverse-phase HPLC and polyacrylamide gel electrophoresis. The fluorescent properties of functionalized DNA were characterized by the steady-state fluorescence measurements.
authors.affiliation: Graduate School of Engineering, University of Hyogo, Himeji, Hyogo 671-2280, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Tanimizu
authors.forename: Tadao
authors.initials: T
authors.lastname: Takada
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kazushige
authors.initials: K
authors.lastname: Yamana
mesh_terms: D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D011721:Pyrenes; D004247:DNA; D004259:DNA-Directed DNA Polymerase