Regulation of the human SLC25A20 expression by peroxisome proliferator-activated receptor alpha in human hepatoblastoma cells.
Solute carrier family 25, member 20 (SLC25A20) is a key molecule that transfers acylcarnitine esters in exchange for free carnitine across the mitochondrial membrane in the mitochondrial beta-oxidation. The peroxisome proliferator-activated receptor alpha (PPARalpha) is a ligand-activated transcription factor that plays an important role in the regulation of beta-oxidation. We previously established tetracycline-regulated human cell line that can be induced to express PPARalpha and found that PPARalpha induces the SLC25A20 expression. In this study, we analyzed the promoter region of the human slc25a20 gene and showed that PPARalpha regulates the expression of human SLC25A20 via the peroxisome proliferator responsive element.
Tachibana
Keisuke
K
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan. nya@phs.osaka-u.ac.jp
Takeuchi
Kentaro
K
Inada
Hirohiko
H
Yamasaki
Daisuke
D
Ishimoto
Kenji
K
Tanaka
Toshiya
T
Hamakubo
Takao
T
Sakai
Juro
J
Kodama
Tatsuhiko
T
Doi
Takefumi
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D045744:Cell Line, Tumor; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D047493:PPAR alpha / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation
D026901:Membrane Transport Proteins; D047493:PPAR alpha; C120109:SLC25A20 protein, human