DOSY NMR applied to analysis of flavonoid glycosides from Bidens sulphurea.
2D DOSY 1H NMR has proved to be a useful technique in the identification of the molecular skeleton of the four major compounds of ethyl acetate extract of aerial parts of Bidens sulphurea (Asteraceae). The combination of this technique with HPLC, mass spectrometry and other NMR techniques enabled the identification of four flavonoid glycosides: quercetin-3-O-beta-D-galactopyranoside, quercetin-3-O-beta-D-glycopyranoside, quercetin-3-O-alpha-L-arabinofuranoside and quercetin-3-O-beta-D-rhamnopyranoside.
Rodrigues
Edilene Delphino
ED
Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida dos Bandeirantes 3900, 14040-901 Ribeirão Preto, SP, Brazil. delphino@pg.ffclrp.usp.br
da Silva
Denise Brentan
DB
de Oliveira
Dionéia Camilo Rodrigues
DC
da Silva
Gil Valdo José
GV
D019659:Asteraceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D012015:Reference Standards
D005419:Flavonoids; D006027:Glycosides