Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis.
Agarose gel electrophoresis is by far the most widely used method for characterizing the topological state of DNA molecules. Although this technique has been used for more than 30 years, the physical mechanism underlying the resolution of topological states remains poorly understood. However, electrophoretic methods remain the most robust and precise techniques for determining the local unwinding of DNA induced by the binding of proteins and small-molecule ligands, analyzing conformational transitions in duplex DNA, measuring changes in helical repeat that accompany shifts in environmental conditions, and characterizing knotting and linking in duplex DNA.
Levene
Stephen D
SD
Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Dallas, TX, USA.
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D026942:DNA Topoisomerases / Q000378:metabolism; D043864:DNA, Catenated / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D045522:Recombinases / Q000378:metabolism; D012984:Software
D043864:DNA, Catenated; D004278:DNA, Superhelical; D010940:Plant Proteins; D045522:Recombinases; D004247:DNA; D026942:DNA Topoisomerases