Preparation of nanobubbles for ultrasound imaging and intracelluar drug delivery.
Echogenic bubble formulations have wide applications in both disease diagnosis and therapy. In the current study, nanobubbles were prepared and the contrast agent function was evaluated in order to study the nanosized bubble's property for ultrasonic imaging. Coumarin-6 as a model drug was loaded into nanobubbles to investigate the drug delivery potential to cells. The results showed that the nanobubbles composed of 1% of Tween 80, and 3 mg/ml of lipid worked well as an ultrasonic contrast agent by presenting a contrast effect in the liver region in vivo. The drug-loaded nanobubbles could enhance drug delivery to cells significantly, and the process was analyzed by sigmoidally fitting the pharmacokinetic curve. It can be concluded that the nanobubble formulation is a promising approach for both ultrasound imaging and drug delivery enhancing.
Wang
Ye
Y
Zhejiang-California International Nanosystems Institute Molecular Imaging Platform, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, PR China. wangyeph@hotmail.com
Li
Xiang
X
Zhou
Yan
Y
Huang
Pengyu
P
Xu
Yuhong
Y
D000818:Animals; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D045423:Microbubbles; D033401:Microscopy, Acoustic / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000378:metabolism
D053769:Nanocapsules; D004364:Pharmaceutical Preparations