The synergistic effects of lysophosphatidic acid receptor agonists and calcitriol on MG63 osteoblast maturation at titanium and hydroxyapatite surfaces.
Successful osseointegration stems from the provision of a mechanically competent mineralised matrix at the implant site. Mature osteoblasts are the cells responsible for achieving this and a key factor for ensuring healthy bone tissue is associated with prosthetic materials will be 1 alpha,25 dihydroxy vitamin D3 (calcitriol). However it is known that calcitriol per se does not promote osteoblast maturation, rather the osteoblasts need to be in receipt of calcitriol in combination with selected growth factors in order to undergo a robust maturation response. Herein we report how agonists of the lysophosphatidic acid (LPA) receptor, LPA and (2S)-OMPT, synergistically co-operate with calcitriol to secure osteoblast maturation for cells grown upon two widely used bone biomaterials, titanium and hydroxyapatite. Efforts could now be focussed on functionalizing these materials with LPA receptor agonists to support in vivo calcitriol-induced osseointegration via heightened osteoblast maturation responses.
Department of Oral & Dental Science, University of Bristol Dental School, Lower Maudlin St., Bristol BS1 2LY, UK. j.p.mansell@bris.ac.uk
Jason P
JP
Mansell
Michele
M
Barbour
Christopher
C
Moore
Maryam
M
Nowghani
Moreica
M
Pabbruwe
Terje
T
Sjostrom
Ashley W
AW
Blom
D000418:Albumins / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology; D063086:Organothiophosphates / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D010712:Phosphatidic Acids / Q000494:pharmacology; D049368:Receptors, Lysophosphatidic Acid / Q000819:agonists*; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*
C473891:1-oleoyl-2-O-methyl-rac-glycerophosphothionate; D000418:Albumins; D001672:Biocompatible Materials; D008246:Lysophospholipids; D063086:Organothiophosphates; D010712:Phosphatidic Acids; D049368:Receptors, Lysophosphatidic Acid; D017886:Durapatite; D014025:Titanium; D002117:Calcitriol; C032881:lysophosphatidic acid