Structural determinants underlying constitutive dimerization of unoccupied human follitropin receptors.
The human follitropin receptor (hFSHR) is a G protein-coupled receptor (GPCR) central to reproductive physiology that is composed of an extracellular domain (ECD) fused to a serpentine region. Using bioluminescence resonance energy transfer (BRET) in living cells, we show that hFSHR dimers form constitutively during their biosynthesis. Mutations in TM1 and TM4 had no effect on hFSHR dimerization, alone or when combined with mutation of Tyr(110) in the ECD, a residue predicted to mediate dimerization of the soluble hormone-binding portion of the ECD complexed with FSH (Q. Fan and W. Hendrickson, Nature 433:269-277, 2005). Expressed individually, the serpentine region and a membrane-anchored form of the hFSHR ECD each exhibited homodimerization, suggesting that both domains contribute to dimerization of the full-length receptor. However, even in the context of only the membrane-anchored ECD, mutation of Tyr(110) to alanine did not inhibit dimerization. The full-length hFSHR and the membrane-anchored ECD were then each engineered to introduce a consensus site for N-linked glycosylation at residue 110. Despite experimental validation of the presence of carbohydrate on residue 110, we failed to observe disruption of dimerization of either the full-length hFSHR or membrane-anchored ECD containing the inserted glycan wedge. Taken altogether, our data suggest that both the serpentine region and the ECD contribute to hFSHR dimerization and that the dimerization interface of the unoccupied hFSHR does not involve Tyr(110) of the ECD.
Department of Molecular Physiology and Biophysics, The University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, United States.
Rongbin
R
Guan
Xueqing
X
Wu
Xiuyan
X
Feng
Meilin
M
Zhang
Terence E
TE
Hébert
Deborah L
DL
Segaloff
D019943:Amino Acid Substitution; D002460:Cell Line; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D006031:Glycosylation; D006801:Humans; D016297:Mutagenesis, Site-Directed; D017434:Protein Structure, Tertiary; D011962:Receptors, FSH / Q000737:chemistry* / Q000378:metabolism
D011962:Receptors, FSH