Follicular dendritic cells in multicentric Castleman disease present human herpes virus type 8 (HHV8)-latent nuclear antigen 1 (LANA1) in a proportion of cases and is associated with an enhanced T-cell response.
Multicentric Castleman disease (MCD) is a rare human herpes virus type 8 (HHV8)-associated lymphoproliferative disorder that occurs more frequently in patients infected with human immunodeficiency virus (HIV). In tissue samples, HHV8-infected plasmablasts localise to the mantle zones of the lymphoid follicles. We revisited the immunohistological features in 25 lymph node (LN) and three spleen samples of MCD. In five (20%) LN and one (33%) spleen sample, HHV8 latent nuclear antigen 1 (LANA1) staining was also noted on the follicular dendritic cells (FDC). The HHV8-positive FDC subgroup of patients had significantly higher numbers of CD3-positive T cells infiltrating the follicles when compared to the HHV8-negative FDC subgroup (P = 0.047). Furthermore, the numbers of HHV8-positive plasmablasts and serum HHV8 viral copy numbers were lower among the HHV8-positive FDC subgroup when compared to the HHV8-negative FDC subgroup (not statistically significant). Our findings show, for the first time, possible 'presentation' of an HHV8 antigen by FDCs in MCD.
Department of Histopathology, Hammersmith Hospital and Imperial College, London, UK.
Hesham
H
El-Daly
Mark
M
Bower
Kikkeri N
KN
Naresh
D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000276:immunology* / Q000378:metabolism; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D005871:Castleman Disease / Q000150:complications / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D005260:Female; D006678:HIV / Q000276:immunology* / Q000378:metabolism; D015658:HIV Infections / Q000150:complications / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D006566:Herpesviridae Infections / Q000150:complications / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D019288:Herpesvirus 8, Human / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000821:virology
D000956:Antigens, Viral; D017252:CD3 Complex; D009687:Nuclear Proteins; C109540:latency-associated nuclear antigen