Determination of full-length cDNA nucleotide sequence of equine carbonic anhydrase VI and its expression in various tissues.
A full-length cDNA clone of an equine carbonic anhydrase (CA)-VI was obtained from the equine parotid gland. The cDNA sequence was 1338 bp long and was predicted to encode a 319 amino acid polypeptide with a putative signal peptide of 18 amino acids. The deduced amino acid sequence of mature CA-VI showed the similarity of 70% to those of other mammalians reported. Westernblot analysis using anti-horse CA-VI peptide detected the single band in parotid gland, and the band reduced its size by treatment with N-glycosidase F. Additionally, CA-VI protein expression was confirmed in submandicular gland and weakly in liver. In contrast, RT-PCR analysis revealed signals in the digestive tract including duodenum, jejunum, ileum, cecum and colon as well as the salivary glands. In addition, certain signals were detected in testis, thyroid gland and liver, but not in nerve tissue, skeletal muscle, spleen or lymph node.
Ochiai
Hideharu
H
Research Institute of Biosciences, Azabu University, Sagamihara, Kanagawa, Japan. ochiaih@azabu-u.ac.jp
Kanemaki
Nobuyuki
N
Kamoshida
Shino
S
Murakami
Masaru
M
Ichihara
Nobutsune
N
Asari
Masao
M
Nishita
Toshiho
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D041981:Gastrointestinal Tract / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D006736:Horses / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013961:Thyroid Gland / Q000378:metabolism; D014018:Tissue Distribution
D018076:DNA, Complementary; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI