Irradiation stability and cytotoxicity of gold nanoparticles for radiotherapy.
Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy. If gold nanoparticles are shown to have good irradiation stability and biocompatibility, they would play an important role in radiotherapy. In this work, we investigated irradiation effects of gold nanoparticles under 2-10 kR gamma irradiation and cytotoxicity of gold nanoparticles with human K562 cells by using Cell Titre-Glo luminescent cell viability assay. The results revealed that gamma irradiation had not induced any obvious instability and size variations in gold nanoparticles. We found that gold nanoparticles showed excellent radiation hardness with an absorbed dose conversation factor of 9.491 rad/R. Meanwhile, the surface plasmon resonance of gold nanoparticles was enhanced obviously after 2-10 kR gamma irradiation. Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability. Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, People's Republic of China.
Xiao-Dong
XD
Zhang
Mei-Li
ML
Guo
Hong-Ying
HY
Wu
Yuan-Ming
YM
Sun
Yan-Qiu
YQ
Ding
Xin
X
Feng
Liang-An
LA
Zhang
D002470:Cell Survival / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D004355:Drug Stability; D005720:Gamma Rays; D006046:Gold / Q000008:administration & dosage* / Q000528:radiation effects*; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage* / Q000528:radiation effects*; D011829:Radiation Dosage; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage* / Q000737:chemistry*
D011838:Radiation-Sensitizing Agents; D006046:Gold