Induction of apoptosis in human leukemia cell (Jurkat) by neolignans isolated from seeds of Licaria puchury-major.
Ethanol extract of the seeds of Licaria puchury-major, a Brazilian herbal medicine, was found to inhibit cell proliferation in human leukemia cell line (Jurkat) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Bioassay-guided fractionation of the active components led to the isolation of one phenylpropanoid (1) and ten neolignans (2-11). The apoptosis-inducing activity of the compounds showing cytotoxicity in Jurkat cells was assessed by flow cytometric analysis. Among the identified neolignans, compounds 3, 4, 6 and 7 which have similar molecular structures, showed apoptotic activity. To elucidate the mechanism of apoptosis induction by neolignans, intracellular caspase-3, -8 and -9 activity in Jurkat cells was evaluated. Compound 4 markedly elevated the activity of caspase-3 and -9.
College of Pharmacy, Nihon University, Narashinodai, Funabashi, Chiba 274-8555, Japan. uchiyama.taketo@nihon-u.ac.jp
Taketo
T
Uchiyama
Keiichi
K
Tabata
Sawako
S
Nomura
Yasuhisa
Y
Kaneko
Yasuo
Y
Fujimoto
Takashi
T
Suzuki
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D019770:Lauraceae / Q000737:chemistry*; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D015394:Molecular Structure; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D020005:Propanols / Q000302:isolation & purification; D012639:Seeds
D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; D010936:Plant Extracts; D020005:Propanols; C439395:1-phenylpropanol; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9