A cost-effective labeling strategy for the NMR study of large proteins: selective 15N-labeling of the tryptophan side chains of prolyl oligopeptidase.
Tarragó
Teresa
T
Institute for Research in Biomedicine, Barcelona Science Park, Baldiri Reixac, 10, 08028 Barcelona, Spain.
Claasen
Birgit
B
Kichik
Nessim
N
Rodriguez-Mias
Ricard A
RA
Gairí
Margarida
M
Giralt
Ernest
E
D000818:Animals; D008958:Models, Molecular; D009587:Nitrogen Isotopes / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D000085822:Prolyl Oligopeptidases; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D012106:Research / Q000191:economics; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry*
D009587:Nitrogen Isotopes; D011506:Proteins; D014364:Tryptophan; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases