In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (Marine Brown Alga).
Water-soluble crude polysaccharide from a brown alga Turbinaria ornata (TCP) was screened for its antioxidant and anti-inflammatory potential. The major functional groups of polysaccharide were analyzed by Fourier Transmission-Infra Red (FT-IR). In vitro free radical quenching and total antioxidant activity of TCP was investigated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide (NO) scavenging, lipid peroxidation (LPO) inhibition and ABTS radical assay. Evaluation of anti-inflammatory activity of TCP was performed using carrageenan-induced paw edema in rats and vascular permeability test in mice. Phytochemical analysis of TCP showed the presence of carbohydrates, proteins and polyphenols further, the FT-IR analysis of TCP showed the presence of functional groups of sugar moiety, uronic acids and sulfate groups. TCP showed maximum LPO, NO and DPPH inhibition of 78.04%, 38.82% and 80.21% at a concentration of 1000, 125 and 500 microg/ml respectively. Oral administration of TCP (2.5, 5, 10, 20mg/kg) reduced the paw edema considerably (p<0.05) in a dose dependent manner compared to carrageenan induced rats. Similarly, oral administration of TCP (3, 10, 30 mg/kg) evoked a significant (p<0.05) dose dependent inhibitory effect on vascular permeability in mice. Altogether, these results suggest that the crude polysaccharide of T.ornata could be considered as a potential antioxidant and anti-inflammatory agent.
Ananthi
Subash
S
Herbal and Indian Medicine Research Laboratory, Department of Biochemistry, Sri Ramachandra University, Porur, Chennai 600 116, India.
Raghavendran
Hanumantha Rao Balaji
HR
Sunil
Adoor Gopalan
AG
Gayathri
Veeraraghavan
V
Ramakrishnan
Ganapathy
G
Vasanthi
Hannah R
HR
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005260:Female; D005528:Foot / Q000473:pathology; D007202:Indicators and Reagents; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D016877:Oxidants / Q000737:chemistry; D000459:Phaeophyceae / Q000737:chemistry*; D010851:Picrates; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D001713:Biphenyl Compounds; D007202:Indicators and Reagents; D016877:Oxidants; D010851:Picrates; D011134:Polysaccharides; D017392:Thiobarbituric Acid Reactive Substances; D009599:Nitroprusside; D009569:Nitric Oxide; D002351:Carrageenan; C004931:1,1-diphenyl-2-picrylhydrazyl