A new chromosome x exon-specific microarray platform for screening of patients with X-linked disorders.
Recent studies and advances in high-density oligonucleotide arrays have shown that microdeletions and microduplications occur at a high frequency in the human genome, causing various genetic conditions including mental retardation. Thus far little is known about the pathways leading to this disease, and implementation of microarrays is hampered by their increasing cost and complexity, underlining the need for new diagnostic tools. The aim of this study was to introduce a new targeted platform called "chromosome X exon-specific array" and to apply this new platform to screening of 20 families (including one blind positive control) with suspected X-linked mental retardation, to identify new causative X-linked mental retardation genes. The new microarray contains of 21,939 oligonucleotides covering 92.9% of all exons of all genes on chromosome X. Patient screening resulted in successful identification of the blind positive control included in the sample of 20 families, and one of the remaining 19 families was found to carry a 1.78-kilobase deletion involving all exons of pseudogene BRAF2. The BRAF2 deletion segregated in the family and was not found in 200 normal male samples, and no copy number variations are reported in this region. Further studies and focused investigation of X-linked disorders have the potential to reveal the molecular basis of human genetic pathological conditions that are caused by copy-number changes in chromosome X genes.
Cyprus Institute of Neurology and Genetics, PO Box 23462, 1683 Nicosia, Cyprus.
Stavros
S
Bashiardes
Ludmila
L
Kousoulidou
Hans
H
van Bokhoven
Hans-Hilger
HH
Ropers
Jamel
J
Chelly
Claude
C
Moraine
Arjan P M
AP
de Brouwer
Hilde
H
Van Esch
Guy
G
Froyen
Philippos C
PC
Patsalis
D041321:Chromosomes, Human, X / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D005091:Exons / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D038901:X-Linked Intellectual Disability / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis