Growth curves of Escherichia coli and Pseudomonas aeruginosa submitted to different ceftazidime concentrations.
Cultures of Escherichia coli and Pseudomonas aeruginosa have been submitted to different ceftazidime concentrations (from 1 to 500 mg/l). A continuous monitoring of the culture was made with the MS-2 system. Phase contrast microscopy was used for study of bacterial morphology. The data obtained showed: for E. coli a drop in optical density and formation of round forms at concentrations close to the minimum inhibitory concentration (MIC) and for Ps. aeruginosa, a residual growth at all concentrations tested during the first 2 h. This corresponded to filament formation. These observations might have clinical implications concerning the mode of administration of the antibiotic, i.e., bolus or continuous infusion.
Yourassowsky
E
E
Department of Clinical Biology, Brugmann University Hospital, 4, Place Van Gehuchten, 1020 Bruxelles, Belgium.
Van der Linden
M P
MP
Lismont
M L
ML
Crokaert
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008858:Microscopy, Phase-Contrast; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development*
D000900:Anti-Bacterial Agents; D002442:Ceftazidime