Effect of sodium selenite on bone repair in tibiae of irradiated rats.
This study evaluated the radioprotective effect of sodium selenite on the bone repair process in tibiae of female rats. For such purpose, 100 female Wistar rats (Rattus norvegicus, albinus) were randomly assigned to 4 groups (n=25), according to the treatment received: administration of distilled water (control); administration of sodium selenite; gamma radiation; and administration of sodium selenite plus gamma radiation. A bone defect was prepared on both tibiae of all animals. Three days after surgery, the gamma radiation and selenium/gamma radiation groups received 8 Gy gamma rays on the lower limbs. Five animals per group were sacrificed 7, 14, 21, 28 days after surgery for evaluation of the repair process by bone volumetric density analysis. The 5 animals remaining in each group were sacrificed 45 days postoperatively for examination of the mature bone by scanning electron microscopy. Based on all analyzed parameters, the results of the present study suggest that sodium selenite exerted a radioprotective effect in the bone repair of tibia of irradiated rats.
Department of Physics, Federal Technological University of Paraná, Curitiba, PR, Brazil.
Anna Silvia Setti da
AS
Rocha
Flávia Maria de Moraes
FM
Ramos-Perez
Frab Norberto
FN
Bóscolo
Flavio Ricardo
FR
Manzi
Mariliani
M
Cchicarelo
Solange Maria
SM
Almeida
D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D008137:Longitudinal Studies; D010027:Osteotomy; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D018038:Sodium Selenite / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D013977:Tibia / Q000187:drug effects / Q000293:injuries / Q000528:radiation effects* / Q000648:ultrastructure; D014945:Wound Healing / Q000187:drug effects / Q000528:radiation effects
D011837:Radiation-Protective Agents; D018038:Sodium Selenite