Production of recombinant anthrax toxin receptor (ATR/CMG2) fused with human Fc in planta.
Mass vaccination against anthrax with existing vaccines is costly and unsafe due to potential side effects. For post-infection treatment, passive immunotherapy measures are currently available, most based on anthrax protective antigen (PA)-specific therapeutic antibodies. Efficient against wild-type strains, these treatment(s) might fail to protect against infections caused by genetically engineered Bacillus anthracis strains. A recent discovery revealed that the von Willebrand factor A (VWA) domain of human capillary morphogenesis protein 2 (CMG2) is an exceptionally effective anthrax toxin receptor (ATR) proficient in helping to resolve this issue. Here we describe in planta production of chimeric recombinant protein (immunoadhesin) comprised of functional ATR domain fused with the human immunoglobulin Fc fragment (pATR-Fc). The fusion design allowed us to obtain pATR-Fc in plant green tissues in a soluble form making it fairly easy to purify by Protein-A chromatography. Standardized pATR-Fc preparations (purity>90%) were shown to efficiently bind anthrax PA as demonstrated by ELISA and Western blot analysis. Recombinant pATR-Fc was also shown to protect J774A1 macrophage cells against the anthrax toxin. This study confirmed that plant-derived pATR-Fc antibody-like protein is a prospective candidate for anthrax immunotherapy.
Andrianov
V
V
Biotechnology Foundation Laboratories at Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
Brodzik
R
R
Spitsin
S
S
Bandurska
K
K
McManus
H
H
Koprowski
H
H
Golovkin
M
M
D001408:Bacillus anthracis / Q000276:immunology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014026:Nicotiana / Q000235:genetics
C447150:ANTXR2 protein, human; D007141:Immunoglobulin Fc Fragments; D008565:Membrane Proteins; D018000:Receptors, Peptide; D011993:Recombinant Fusion Proteins; C070309:anthrax toxin receptors