[Genotypic characteristics of Neisseria meningitidis serogroup W-135 strains isolated as the agent of fatal meningitis in a military hospital].
The aim of this study was to describe the genetic characterization of a total of 6 Neisseria meningitidis serogroup W-135 strains isolated from patients with meningitis and carriers in a military hospital in 2007-2008. Suspected colonies on modified Thayer-Martin medium plates were screened for oxidase reactivity and Gram stain. If gram-negative diplococci were present, a biochemical profile by the API NH system was used for species confirmation. Pulse field gel electrophoresis typing of Nhel-digested DNA was performed by a previously described method. Multi-locus sequence typing (MLST) was performed using the standard primers as listed on the Neisseria MLST website. Three distinct sequence types (STs) were identified: ST-11, ST-2754, ST-3751. One of the clinical isolates was identified as the same sequence type with Hajj isolate (ST-11) and the isolate with ST-2754 was the same as the first Turkish clinical strain isolated in 2003. These data demonstrated that along with ST-11 which is a known Hajj isolate, the ST-2754 strain causing meningococcal disease in Turkey beginning from the year 2003, should be carefully monitored.
Kiliç
Abdullah
A
Jolley
Keith A
KA
Beşirbellioğlu
Bülent
B
Koçak
Nafiz
N
Bedir
Orhan
O
Eyigün
Can Polat
CP
Başustaoğlu
Ahmet Celal
AC
D002353:Carrier State / Q000382:microbiology; D005838:Genotype; D006772:Hospitals, Military; D006801:Humans; D008585:Meningitis, Meningococcal / Q000382:microbiology* / Q000401:mortality*; D008889:Military Personnel; D038582:Neisseria meningitidis, Serogroup W-135 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014421:Turkey / Q000453:epidemiology