Role of ORF74-encoded viral G protein-coupled receptor in human herpesvirus 8 lytic replication.
The human herpesvirus 8 (HHV-8) viral G protein-coupled receptor (vGPCR) has been implicated in virus-associated disease pathogenesis due principally to its ability to induce the production of angiogenic cytokines involved in this process. However, the role of the vGPCR in normal virus biology is understudied and remains unknown. Here we provide evidence from vGPCR gene knockout and depletion experiments that vGPCR is a positive regulator of HHV-8 productive replication and, through experimental utilization of Galpha-coupling variants of vGPCR, that signaling via Galpha(q) activation and targeted mitogen-activated protein kinase pathways is of particular relevance to this activity.
Sandford
Gordon
G
Sidney Kimmel Comprehensive Cancer Center, Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Choi
Young Bong
YB
Nicholas
John
J
D019288:Herpesvirus 8, Human / Q000502:physiology*; D006801:Humans; D019707:Receptors, Chemokine / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000502:physiology*; D014779:Virus Replication
C115448:G protein-coupled receptor, Human herpesvirus 8; C105181:ORF74 protein, Human herpesvirus 8; D019707:Receptors, Chemokine; D014764:Viral Proteins