The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism.
In recent years, research in the areas of stem cells has dramatically increased, including studies of cellular adhesion to a substrate. We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. The adhesion of hASCs to collagens and laminin was completely inhibited by a monoclonal antibody, Mab 2253, which binds to the beta1 integrin subunit. These data indicate that hASC adhesion to collagens and laminin was exclusively mediated by an integrin. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP alone. These results indicate that both the beta1 subunit and the heparan sulfate proteoglycan participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN substrate. These results suggest that hASCs have an adhesion mechanism for the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly correlated with adipogenic differentiation.
Biomaterial Research Center, Division of Life Sciences, Korea Institute of Science and Technology, 39-1 Hawolgok, Seongbuk, Seoul 136-791, Republic of Korea.
In-Su
IS
Park
Min
M
Han
Jong-Won
JW
Rhie
Soo Hyun
SH
Kim
Youngmee
Y
Jung
Ik Hwan
IH
Kim
Sang-Heon
SH
Kim
D050156:Adipogenesis / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology*; D002448:Cell Adhesion / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D008875:Middle Aged; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019012:Integrin beta1