Homing of lin(-)/CD117(+) hematopoietic stem cells.
In this report, we describe the homing of hematopoietic stem cells (HSCs) to non-hematopoietic tissues in lethally irradiated (9Gy) hybrid mice transplanted intravenously with lin(-)/CD117(+) bone marrow cells from ROSA26 mice. The numbers of CFU-GM in spleen of irradiated transplanted mice were well above the levels found in non-irradiated group as early as day 8 after transplant. On 12th day regeneration of lymphocytes was observed, an increase in granulocytes was detected as late as on 33rd day. Transplanted cells containing lacZ gene were detected in recipient mice by histochemistry and their location in the thymus, liver, stomach and ileum was followed during 33days post-transplantation. On 8 and 33days post-transplantation, we found massive presence of donor (lacZ(+)) cells in the thymic cortex. Hematopoietic stem cell transplantation led not only to recovery of hematopoietic and lymphoid tissues but also facilitated recovery of the small intestinal mucosa, which was significantly damaged by ionizing radiation.
Department of Oncology and Radiotherapy, Charles University in Prague, Faculty of Medicine and Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic. filip@fnhk.cz
Stanislav
S
Filip
Jaroslav
J
Mokrý
Jirina
J
Vávrová
Dana
D
Cízková
Zuzana
Z
Sinkorová
Vlasta
V
Tosnerová
Milan
M
Bláha
D000818:Animals; D002465:Cell Movement; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006413:Hematopoietic System / Q000502:physiology*; D007413:Intestinal Mucosa / Q000502:physiology; D007421:Intestine, Small; D007763:Lac Operon; D008221:Lymphoid Tissue / Q000502:physiology*; D051379:Mice; D019009:Proto-Oncogene Proteins c-kit; D012038:Regeneration; D013997:Time Factors; D014018:Tissue Distribution; D014916:Whole-Body Irradiation
D019009:Proto-Oncogene Proteins c-kit