Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.
TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TNF receptors on the heterogeneous rheumatoid synovial cells. Using this system, we detected a higher population of macrophages and a greater TNF receptor expression on the synovial macrophages derived from a synovectomy in comparison to the findings obtained from knee joint replacement surgery.
First Department of Internal Medicine, Nagasaki University Hospital, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan, idah@net.nagasaki-u.ac.jp.
Hiroaki
H
Ida
Toshiyuki
T
Aramaki
Hideki
H
Nakamura
Keita
K
Fujikawa
Kazuhiko
K
Arima
Mami
M
Tamai
Makoto
M
Kamachi
Katsuya
K
Satoh
Tomoki
T
Origuchi
Atsushi
A
Kawakami
Itaru
I
Furuichi
Yojiro
Y
Kawabe
Katsumi
K
Eguchi