[Laser tweezers Raman spectroscopy analysis of liver cancer tissue].
Single cell laser tweezers Raman spectroscopy (LTRS) has been applied to biology field. In the present article, the authors measured the spectra of liver cancer cells, para-cancer cells and normal hepatocytes using single cell laser tweezer Raman spectroscopy (LTRS) system and compared their average spectra changes. The results showed that the laser tweezers Raman spectroscopy could differentiate specimens of different pathological changes from liver tissue studied. The 1 070 and 1 266 cm(-1) peaks obtained from normal hepatocytes were more visible than the same two peaks obtained from liver cancer and para-cancer specimen. The 1 445 cm(-1) peak of normal hepatocytes was higher than that of liver cancer cells and para-cancer cells. It is known that the 1 070 cm(-1) peak represents lipids and nucleic acids, while 1 266 and 1 445 cm(-1) peaks represent lipids and proteins. So, these peak changes may directly reflect the changed biomaterials related to liver carcinogenesis. Thus, single cell laser tweezer Raman spectroscopy may be a nondestructive, rapid and good method to measure and analyze different pathological specimens from liver cancer.
State Key Lab of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. wangyanjun117@yahoo.com.cn
Yan-Jun
YJ
Wang
Hui-Lu
HL
Yao
Gui-Wen
GW
Wang
Yun
Y
Wang
Mei-Fu
MF
Feng
D006801:Humans; D008099:Liver / Q000166:cytology / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology*; D052898:Optical Tweezers; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*