Production of tylosin in solid-state fermentation by Streptomyces fradiae NRRL-2702 and its gamma-irradiated mutant (gamma-1).
OBJECTIVE
To develop solid-state fermentation system (SSF) for hyper production of tylosin from a mutant gamma-1 of Streptomyces fradiae NRRL-2702 and its parent strain.


RESULTS
Various agro-industrial wastes were screened to study their effect on tylosin production in SSF. Wheat bran as solid substrate gave the highest production of 2500 microg of tylosin g(-1) substrate by mutant gamma-1 against parent strain (300 microg tylosin g(-1) substrate). The tylosin yield was further improved to 4500 microg g(-1) substrate [70% moisture, 10% inoculum (v/w), pH 9.2, 30 degrees C, supplemental lactose and sodium glutamate on day 9]. Wild-type strain displayed less production of tylosin (655 microg of tylosin g(-1) substrate) in SSF even after optimization of process parameters.


CONCLUSIONS
The study has shown that solid-state fermentation system significantly enhanced the tylosin yield by mutant gamma-1.


CONCLUSIONS
This study proved to be very useful and resulted in 6.87 +/- 0.30-fold increase in tylosin yield by this mutant when compared to that of wild-type strain.
Khaliq
Shazia
S
Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Rashid
Nosheen
N
Akhtar
Kalsoom
K
Ghauri
Muhammad Afzal
MA
D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D005285:Fermentation; D005720:Gamma Rays; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation / Q000187:drug effects*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D015645:Tylosin / Q000096:biosynthesis*
D003470:Culture Media; D015645:Tylosin