Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles.
The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs-KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20-800 nmol L(-1) and 2 nmol L(-1), respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94-104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs-KANA system, may provide a new approach for determination of compounds rich in OH groups.
The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Xinyi
X
Wang
Mingjian
M
Zou
Xiao
X
Xu
Rong
R
Lei
Kean
K
Li
Na
N
Li
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000652:urine; D006046:Gold; D006801:Humans; D007612:Kanamycin / Q000737:chemistry / Q000652:urine*; D053768:Metal Nanoparticles; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D014508:Urea
D000900:Anti-Bacterial Agents; D007612:Kanamycin; D006046:Gold; D014508:Urea