Fabrication of mesoporous SiO(2)-C-Fe(3)O(4)/gamma-Fe(2)O(3) and SiO(2)-C-Fe magnetic composites.
A synthetic method for the fabrication of silica-based mesoporous magnetic (Fe or iron oxide spinel) nanocomposites with enhanced adsorption and magnetic capabilities is presented. The successful in situ synthesis of magnetic nanoparticles is a consequence of the incorporation of a small amount of carbon into the pores of the silica, this step being essential for the generation of relatively large iron oxide magnetic nanocrystals ( approximately 10+/-3nm) and for the formation of iron nanoparticles. These composites combine good magnetic properties (superparamagnetic behaviour in the case of SiO(2)-C-Fe(3)O(4)/gamma-Fe(2)O(3) samples) with a large and accessible porosity made up of wide mesopores (>9nm). In the present work, we have demonstrated the usefulness of this kind of composite for the adsorption of a globular protein (hemoglobin). The results obtained show that a significant amount of hemoglobin can be immobilized within the pores of these materials (up to 180mgg(-1) for some of the samples). Moreover, we have proved that the composite loaded with hemoglobin can be easily manipulated by means of an external magnetic field.
Instituto Nacional del Carbón (CSIC), Apartado 73, 33080-Oviedo, Spain.
Marta
M
Sevilla
Teresa
T
Valdés-Solís
Pedro
P
Tartaj
Antonio B
AB
Fuertes
D000327:Adsorption; D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000138:chemical synthesis / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D009584:Nitrogen / Q000737:chemistry; D016062:Porosity; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000737:chemistry; D013499:Surface Properties; D014961:X-Ray Diffraction
D004337:Drug Carriers; D005290:Ferric Compounds; D006454:Hemoglobins; C509969:SBA-15; C000499:ferric oxide; D013395:Sucrose; D058428:Silica Gel; D002244:Carbon; D012822:Silicon Dioxide; D009584:Nitrogen; D052203:Ferrosoferric Oxide