[Arbovirus investigation in some regions of Shanxi province in 2007].
OBJECTIVE
To investigate arboviruses in some regions of Shanxi province, isolation and identification for arbovirus activity from mosquitoes was conducted.


METHODS
Mosquitoes were collected from these area in 2007 and then used for virus isolation by cell culture. The virus isolates were identified by molecular biology and the sequences were analyzed by bioinformatics.


RESULTS
Ten Banna virus (strains SX0765, SX0766, SX0767, SX0771, SX0789, SX0790, SX0793, SX0794, SX0795, SX0796) were isolated, and two Liaoning virus were also isolated from isolates SX0771, SX0794. Phylogenetic tree of the Banna virus isolates showed that ten strains are located in a distinct branch from all of the other Chinese Banna virus isolates. The homology is between 89.7% and 94.1%.


CONCLUSIONS
Ten Banna virus and two Liaoning virus were isolated during this arbovirus investigation in Shanxi province. New Banna virus isolates showed a distinct phylogenetic relationship with the other Chinese Banna virus strains.
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Ming-Hua
MH
Li
Wei-Shan
WS
Meng
Shi-Hong
SH
Fu
Jing-Xia
JX
Cheng
Jun-Ying
JY
Zhao
Xiang-Sheng
XS
Kong
Pei-Fang
PF
Dai
Guo-Dong
GD
Liang
D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002460:Cell Line; D002681:China; D009033:Culicidae / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny