Towards profiling the gene expression of tyrosinase-induced melanogenesis in HEK293 cells: a functional DNA chip microarray and interactomics studies.
The overexpression of a single tyrosinase gene can induce conspicuous pigmentation in nonpigmented cells. We hypothesized that some unknown tyrosinase-associated genes are simultaneously regulated by melanin synthesis. To improve understanding of melanogenesis and tyrosinase-associated functions, we attempted to profile the genes that are altered during melanin production in HEK293 cells by using a functional DNA chip microarray. The candidate genes were obtained based on significance analysis of microarray (SAM) and further computational prediction via protein-protein interaction (PPI) mapping suggested that newly detected hub genes were involved in melanogenesis. PPI mapping using bioinformatic tools revealed 8 genes that formed an interaction hub. The yeast two-hybridization results suggested some candidate genes could interact with tyrosinase. The present study provides information to further understand the complex factors associated with tyrosinase-induced melanogenesis and apoptosis. The approach of combining expression data analysis and predicted protein interaction partners can help identify genes involved in pigmentation.
Department of Biotechnology, Yonsei University, Seoul, Korea.
Ick-Hyun
IH
Cho
Zhi-Rong
ZR
Lü
Jae-Ran
JR
Yu
Yong-Doo
YD
Park
Jun-Mo
JM
Yang
Myong-Joon
MJ
Hahn
Fei
F
Zou
D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008543:Melanins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D014442:Monophenol Monooxygenase / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D012880:Skin Pigmentation / Q000235:genetics
D008543:Melanins; D012333:RNA, Messenger; D014442:Monophenol Monooxygenase