Molecular characterization and marker based chemotaxonomic studies of Podophyllum hexandrum Royle.
Detailed chemical studies and RAPD analysis were done in different populations of Podophyllum hexandrum collected from high altitude regions of North Western Himalayas. Random amplified polymorphic DNA (RAPD) analysis revealed a high degree of genetic diversity among the 12 collected accessions, attributed to their geographical and climatic conditions. HPLC analysis also revealed variation in the concentration of two major marker compounds which lead to the identification of a chemotype. The study demonstrated that RAPD and chemical markers are very useful tools to compare the genetic relationship and pattern of variation among such prioritized and endangered medicinal plants.
Division of Biotechnology, Indian Institute of Integrative Medicine, formerly, RRL-(CSIR), Sanatnagar, Srinagar 190005, Jammu and Kashmir, India. bioshakir@gmail.com
Phalisteen
P
Sultan
A S
AS
Shawl
Suriya
S
Rehman
S Fayaz
SF
Ahmed
P W
PW
Ramteke
D000531:Altitude; D000970:Antineoplastic Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002980:Climate; D016172:DNA Fingerprinting; D018744:DNA, Plant; D014644:Genetic Variation; D005843:Geography; D006027:Glycosides / Q000032:analysis; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry* / Q000235:genetics; D011034:Podophyllotoxin / Q000032:analysis / Q000235:genetics; D011035:Podophyllum / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D000970:Antineoplastic Agents; D018744:DNA, Plant; D006027:Glycosides; D011034:Podophyllotoxin