Altered protein-expressing profile in hPNAS4-induced apoptosis in A549 human lung adenocarcinoma cells.
Human PNAS4 (hPNAS4) is a recently identified pro-apoptosis gene, which is able to induce apoptosis in A549 human lung adenocarcinoma cells following its overexpression. In this work, we investigated the changes of protein profile in hPNAS4-induced apoptosis in A549 cells through proteomic strategy consisting of two-dimensional electrophoresis (2-DE) coupled with MALDI-Q-TOF mass spectrometry. A total of 20 different proteins with more than 3.0-fold change in expression, including 5 up-regulated and 15 down-regulated proteins were successfully identified by database search. The mRNA transcription levels of the different proteins were further examined by RT-PCT. Functional analyses showed these different proteins are involved in diverse biological processes including metabolism, proteolysis, signal transduction, apoptosis, and redox regulation. Two essential apoptosis-associated protein, annexin A1 and prothymosin alpha, were confirmed by Western blot and showed consistent changes with proteomic detection. Our data provide molecular evidence and possible associated pathway in hPNAS4-induced apoptosis through proteomic strategy, which should be contributed to further investigation on biological function of hPNAS4.
Gou
Lan-Tu
LT
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, Sichuan Province, China.
Tong
Ai-Ping
AP
Yan
Fei
F
Yuan
Zhu
Z
He
Fei
F
Wang
Wei
W
Zhou
Yan
Y
Chen
Li-Juan
LJ
Tang
Ming-Hai
MH
Yang
Jin-Liang
JL
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017305:Annexin A1 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D019759:Carbon-Nitrogen Lyases; D045744:Cell Line, Tumor; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010084:Oxidation-Reduction; D011498:Protein Precursors / Q000378:metabolism; D015398:Signal Transduction; D013947:Thymosin / Q000031:analogs & derivatives / Q000378:metabolism; D015854:Up-Regulation
D017305:Annexin A1; D051017:Apoptosis Regulatory Proteins; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin; D019759:Carbon-Nitrogen Lyases; C000604991:DESI2 protein, human