[Effect of Dahuang Zhechong pills on arterial thrombosis in rabbits].
OBJECTIVE
To investigate the effect of Dahuang Zhechong pills (DZ) on arterial thrombotic model in vivo.


METHODS
Sixty-five rabbits were randomly divided into 7 groups: normal, model (collagen encapsulated thread-drawing),model+aspirin (ASA), model+clopidogrel (CP),model+ASA+CP, model+ low dosage DZ (DZL), and model+high dosage DZ (DZH). All rabbits except the normal group were fed with the drugs repectively for 8 days,and sacrificed at 2 hours after the last feeding, obtained aortae. The pathological changes in the aortae were observed under microscope,and the level of FDP, D-dimer and tissue factor (TF) were measured by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The vascular vessels were filled with thrombi in the model group and the elastic membranes of the vessel wall were seriously injured. The arterial thrombi were observed around the vascular wall in the DZL group, but some of the thrombi were dissolved. The number of thrombi was remarkably decreased in the DZH group, and most thrombi were dissolved and the vascular intimal membranes were intact. Compared with the model group, the dry and wet weight of the thrombi and the level of D-dimer, FDP, and TF in the plasma were significantly attenuated (P<0.01) in all the treatment groups. There were no significant difference between the DZL group and the ASA group in the dry weight, D-Dimer, and FDP (P>0.05). The pathological changes in the vascular vessel and the elevation of plasma parameters in the DZL group were similar to those in the ASA and CP groups (P>0.05). The dry and wet weight, D-dimer, FDP, and TF in the plasma in the DZH group were significantly lower than those in the DZL group (P<0.01 or P<0.05, separatively), and closed to those in the ASA+CP group.


CONCLUSIONS
Dahuang Zhechong pills are potential novel anti-thromobotic agent for arterial thrombosis.
Department of Integrated Chinese and Western Medicine, Engineering Center of Screening Traditional Chinese Medicine, Xiangya Hospital, Central South University, Changsha, China. wds66@yahoo.com.cn
Dongsheng
D
Wang
Fangping
F
Chen
Faqing
F
Tang
Juntao
J
Feng
Shilin
S
He
Xilian
X
Huang
Changjiang
C
Xiao
D000818:Animals; D017536:Carotid Artery, Common / Q000378:metabolism / Q000473:pathology*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D008297:Male; D008517:Phytotherapy; D011817:Rabbits; D011897:Random Allocation; D013925:Thromboplastin / Q000378:metabolism; D013927:Thrombosis / Q000188:drug therapy*
C521634:Dahuang Zhechong pill; D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D013925:Thromboplastin