RhoB enhances migration and MMP1 expression of prostate cancer DU145.
Rho family protein regulates variety of cellular functions as cytoskeletal organization, cell proliferation and apoptosis. In the present study, we demonstrate that RhoB-overexpressed prostate cancer cells showed an enhanced cell motility and the administration of the GSK-3 inhibitors inhibited this increase in migration. Among the extracellular matrix and adhesion-related molecules, MMP1 RNA expression was increased in RhoB-overexpressed cells, administration of MMP inhibitor suppressed the collagen gel invasion in these cells. This is the first report evaluating RhoB function and the downstream signaling events in prostate cancer cell. Our results indicate that RhoB promotes cell motility and invasion in a metastatic prostate cancer cell.
Yoneda
Misao
M
Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20 Minami, Daiko, Higashi-ku, Nagoya City, Aichi, Japan.
Hirokawa
Yoshifumi S
YS
Ohashi
Atsuyuki
A
Uchida
Katsunori
K
Kami
Daisuke
D
Watanabe
Masatoshi
M
Yokoi
Toyoharu
T
Shiraishi
Taizo
T
Wakusawa
Shinya
S
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D020743:rhoB GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D038362:Glycogen Synthase Kinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D020743:rhoB GTP-Binding Protein