Fabrication of a novel impedance cell sensor based on the polystyrene/polyaniline/Au nanocomposite.
Gold nanoparticles (AuNPs) were assembled on the surface of polystyrene (PS) and polyaniline (PANI) core-shell nanocomposite (PS@PANI) for the immobilization of HL-60 leukemia cells to fabricate a cell electrochemical sensor. The immobilized cells exhibited irreversible voltammetric response and increased the electron transfer resistance with a good correlation to the logarithmic value of concentration ranging from 1.6 x 10(3) to 1.6 x 10(8) cells mL(-1) with a limit of detection of 7.3 x 10(2) cells mL(-1) at 10 sigma. This biosensor was simple, low cost and disposable, which implied that the PS@PANI/Au composites can regard as the potential applications for clinical applications.
Gu
Miaomiao
M
Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Zhang
Jingjing
J
Li
Ye
Y
Jiang
Liping
L
Zhu
Jun-Jie
JJ
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D018914:Cells, Immobilized; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011137:Polystyrenes / Q000737:chemistry*
D000814:Aniline Compounds; D011137:Polystyrenes; C416807:polyaniline; D006046:Gold