A single-nucleotide polymorphism marker within the FCRL5 gene and HLA-B27 positive Han Chinese ankylosing spondylitis patients.
The aim of this study was to determine whether FCRL5 genes in concert with human leukocyte antigen-B27 (HLA-B27) genotypes are associated with susceptibility to ankylosing spondylitis (AS) in Chinese population. One hundred and sixty-nine HLA-B27-positive AS patients (107 males and 62 females) and 184 HLA-B27-positive matched controls (112 males and 72 females) were analyzed from Han Chinese populations by case-control design, and their samples were genotyped using a panel of two single-nucleotide polymorphism (SNP) markers (rs6427384, rs12036228) within the FCRL5 gene by ligase detection reactions (LDRs) and the HLA-B27 subtypes were determined by polymerase chain reaction (PCR) using sequence-specific primer (SSP) methods. Our results show that in addition to B27, the SNPs rs6427384 and rs12036228 were associated with AS, and the C-T haplotype was higher in cases with AS than in the control population [74.8% vs 63.6%, Fisher's P = 0.003, odds ratio (OR) = 1.660,95% confidence interval (CI) = 1.184-- 2.326]. Our results suggest that, in addition to HLA-B27, a novel polymorphism within the FCRL5 gene confers susceptibility to AS in Han Chinese population.
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Anhui, China.
X
X
Tang
F
F
Pan
G
G
Xia
F
F
Liao
R
R
Ge
Y
Y
Mei
D
D
Ye
S
S
Xu
J
J
Xu
D000328:Adult; D044466:Asian People; D016022:Case-Control Studies; D002681:China; D005260:Female; D015796:HLA-B27 Antigen / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*; D011961:Receptors, Fc; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000276:immunology / Q000821:virology
C432367:FCRL5 protein, human; D015796:HLA-B27 Antigen; D011956:Receptors, Cell Surface; D011961:Receptors, Fc