Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.
We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associated with processing film.
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA.
Mitri K
MK
Khoury
Ian
I
Parker
Dana W
DW
Aswad
D015153:Blotting, Western / Q000379:methods*; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008670:Metals / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors; D014962:X-Ray Film
D008670:Metals; D010087:Oxides