[Study of copper metabolism and liver damage in TX Mice-an animal model for liver disease].
OBJECTIVE
To provide right time points in selection of right aged animals and the normal physiological data of TX mice.


METHODS
7-12 months old TX and DL mice were studied, each group contained 3 female and 3 male mice of TX or DL mice. The concentration of copper in the serum, dry tissues (liver, brain and kidney), together with copper biochemistry indexes were measured. The liver histopathology was observed under light microscopy and electron microscope.


RESULTS
Transaminase increased significantly only in 10 and 11-month- old (AST(TX10) = 218.3 U/L, AST(TX11) = 197.5 U/L, AST(DL10) = 171.5 U/L, AST(DL11) = 165.0 U/L, P(10) less than 0.001, P(11) = 0.022), but the copper concentration of liver, brain and kidney was significantly increased during 7-12 month old (the average concentration of copper, Liver(TX) = (750.0 +/- 85.5) mg/kg, Brain(TX) = (39.7 +/- 2.2)mg/kg, Kidney(TX) = (29.8 +/- 5.0) mg/kg, Liver(DL) = (11.6 +/- 1.5) mg/kg, Brain(DL) = (16.8 +/- 0.9) mg/kg, Kidney(DL) = (14.2 +/- 1.0) mg/kg, t = 21.16, 23.60, 7.47, for all these organs P less than 0.05).


CONCLUSIONS
TX mice is a suitable model of liver disease with natural recovery, so selecting animal model of suitable time point is very important.
Department of Rehabilitation Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Xi
X
Chen
Chu-huai
CH
Wang
Yan-qing
YQ
Feng
Qi-qiang
QQ
Tang
Qiu-you
QY
Xie
Qi
Q
Liang
Xiu-ling
XL
Liang
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001921:Brain / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008107:Liver Diseases / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013997:Time Factors
D003300:Copper; D002570:Ceruloplasmin; D001219:Aspartate Aminotransferases