[Evaluation of the ELISA and the enhanced chemiluminescence immunoassay in use to determine the serum markers for liver fibrosis].
OBJECTIVE
To evaluate the detection method of ELISA and Enhanced Chemiluminescence Immunoassay (ECLIA) in use to determine serum hyaluronate acid (HA), laminin (LN), type IV collagen (IV-C) and type III procollagen (PC III).


METHODS
253 patients with chronic hepatitis B were determined the four liver fibrosis serum markers with both the ECLIA and ELISA, and then compared with pathology results separately.


RESULTS
Both the detection results of ELISA and ECLIA can reflect that the patient's liver fibrosis from hepatitis to liver cirrhosis aggravated gradually. Compared with ELISA, the results of ECLIA and pathology have a better correlation.


CONCLUSIONS
The detection of four liver fibrosis serum markers by ECLIA could indicate the better the response of the state of live fibrosis.
Center of Clinical Laboratory, The No. 302 Hospital of The PIA, Beijing 100039, China.
Jing-Xia
JX
Guo
Hong-Bin
HB
Ma
Yong-Li
YL
Li
Jun
J
Xu
Li-Hua
LH
Yang
Jia-Bin
JB
Shi
Bo
B
Li
Ai-Xia
AX
Liu
Xiao-Xi
XX
Li
Bo-An
BA
Li
Yuan-Li
YL
Mao
D015415:Biomarkers / Q000097:blood*; D024061:Collagen Type III / Q000097:blood; D024141:Collagen Type IV / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007797:Laminin / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D008163:Luminescent Measurements / Q000379:methods*
D015415:Biomarkers; D024061:Collagen Type III; D024141:Collagen Type IV; D007797:Laminin