[Phage display random peptide library for screening the peptides that specifically bind to hepatocellular carcinoma cells].
OBJECTIVE
To screen the peptides that bind specifically to the hepatoma cells using phage display random peptides library.


METHODS
Three rounds of panning were conducted in vitro targeting HepG2 cell lines. In nude mice bearing HepG2 tumor, one round of panning was conducted, and 30 phage clones were randomly selected for sequence analysis to identify the consensus sequence. Immunocytochemistry and immunohistochemistry were performed to determine the specificity of the phages to the hepatoma cells.


RESULTS
After three rounds of panning in vitro and one round of panning in vivo, the phages binding to HepG2 cells were enriched. Sequence analysis of the randomly selected clones identified the peptide sequence VRKRSECLGAHD as the most frequent sequence. Immunocytochemistry and immunohistochemistry confirmed the specificity of the phage binding to the hepatoma cells.


CONCLUSIONS
Specific peptides against hepatoma cells can be obtained from a phage- display peptide library, which provides an experimental basis for developing therapeutic agents targeting hepatoma cells.
Yang
Pu
P
National Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, Changsha, China. yangpu1981@gamil.com
He
Jian-tai
JT
Wang
Ji-wei
JW
Hu
Yu
Y
Yu
Li
L
Jin
Xin
X
Zhang
Yang-de
YD
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D051379:Mice; D009368:Neoplasm Transplantation; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding
D019151:Peptide Library; D010455:Peptides