Extractive fermentation in cloud point system for lipase production by Serratia marcescens ECU1010.
Extractive microbial fermentation for production of lipase by Serratia marcescens ECU1010 has been carried out in cloud point system. The cloud point system is composed of mixture nonionic surfactants with a ratio of Triton X-114 to Triton X-45 4:1 in aqueous solution. The lipase prefers to partition into the surfactant rich phase (coacervate phase) whereas the cells and other hydrophilic proteins retain in the dilute phase of cloud point system. Thus, a concentration factor 4.2-fold and a purification factor 1.3-fold of the lipase have been achieved in the extractive fermentation process. This is the first report about extractive fermentation of proteins in cloud point system.
Pan
Tao
T
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People's Republic of China.
Wang
Zhilong
Z
Xu
Jian-He
JH
Wu
Zhenqiang
Z
Qi
Hanshi
H
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003902:Detergents; D005285:Fermentation; D008049:Lipase / Q000096:biosynthesis*; D017830:Octoxynol; D011092:Polyethylene Glycols; D012706:Serratia marcescens / Q000201:enzymology* / Q000254:growth & development; D013501:Surface-Active Agents
D001426:Bacterial Proteins; D003902:Detergents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D008049:Lipase