Recombinant sendai virus-mediated gene transfer to mouse pancreatic stem cells.
Efficient gene transfer into stem cells is essential for the basic research and for therapeutic applications in gene-modified regenerative medicine. Adenovirus (AdV) vectors, one of the most commonly used types of vectors, can mediate high, albeit transient, levels of expression of the transgene in pancreatic stem/progenitor cells. However, high multiplicity of infection (MOI) with AdV vectors can result in cellular toxicity. Therefore, AdV vectors have been of limited usefulness in clinical applications. In this study, we investigated the in vitro gene transfer efficiency of Sendai virus (SeV) vectors, a paramyxovirus vector that can efficiently introduce foreign genes without toxicity into several cell types, including pancreatic stem cells. The dose-dependent GFP expression of pancreatic stem cells transfected with SeV vectors after 48 h of culture at 37 degrees C was observed. The transfection of pancreatic stem cells with SeV vectors and AdV vectors results in equal expression of the transgene (GFP expression) in the cells after 48 h of culture at 37 degrees C. Although the transfection of pancreatic stem cells with AdV vectors at high MOIs was cytotoxic, transfection with SeV vectors at high MOIs was rarely cytotoxic. In addition, pancreatic stem cells transfected with SeV maintained their differentiation ability. These data suggest that SeV could provide advantages with respect to safety issues in gene-modified regenerative medicine.
Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Koichi
K
Oishi
Hirofumi
H
Noguchi
Hiroshi
H
Yukawa
Makoto
M
Inoue
Soichi
S
Takagi
Hisashi
H
Iwata
Mamoru
M
Hasegawa
Shuji
S
Hayashi
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000633:toxicity; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010179:Pancreas / Q000166:cytology*; D010183:Pancreatic Ducts / Q000166:cytology; D011995:Recombination, Genetic; D029082:Sendai virus / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology*; D014161:Transduction, Genetic / Q000379:methods*
D007328:Insulin; D049452:Green Fluorescent Proteins