Isolation and characterization of a GDSL esterase from the metagenome of a marine sponge-associated bacteria.
Using a metagenome library constructed from a bacterial associated with a marine sponge Hyrtios erecta, we identified a novel esterase that belongs to the SGNH hydrolase superfamily of esterases. The substrate specificity of EstHE1 was determined using p-nitrophenyl (pNP) ester (C2: acetate, C4: butylate, C6: caproate, C12: laurate, C16: palmitate). EstHE1 exhibited activity against C2 (5.6 U/mg), C4 (5.1 U/mg), and C6 (2.8 U/mg) substrates. The optimal temperature for EstHE1 esterase activity of the pNP acetate substrate was 40 degrees C, and EstHE1 retained 60% of its enzymatic activity in the 30-50 degrees C range. This esterase showed moderate thermostability, retaining 58% of its activity even after preincubation for 12 h at 40 degrees C. EstHE1 also maintained activity in high concentrations of NaCl, indicating that this esterase is salt-tolerant. Thus, EstHE1 has the thermal stability and salt tolerance necessary for use as an industrial enzyme.
Department of Life Science and Medical Bio-Science, Waseda University, 2-2 Wakamatsu-cho, Shinjuku, Tokyo 162-8480, Japan. yoshiko_okamura@aoni.waseda.jp
Yoshiko
Y
Okamura
Tomonori
T
Kimura
Hiroko
H
Yokouchi
Macarena
M
Meneses-Osorio
Masaya
M
Katoh
Tadashi
T
Matsunaga
Haruko
H
Takeyama
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004950:Esterases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial / Q000235:genetics*; D006868:Hydrolysis; D054892:Metagenome / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D012492:Salts / Q000494:pharmacology; D016415:Sequence Alignment; D013696:Temperature
D004791:Enzyme Inhibitors; D005227:Fatty Acids; D012492:Salts; D004950:Esterases