[The association of TCR-mutant cells with DNA polymorphism in women residing in radiation polluted regions of the Russian Federation].
Using flow-cytometric method the frequency of lymphocytes beaming mutations at T-cell receptor (TCR) locus was assessed in women residing in radiation polluted regions of Bryansk and Tula Districts. Simultaneously genotyping of the 8 polymorph loci for genes involved in detoxication of xenobiotics and oestrogen metabolism was carried out. The increased TCR-mutant cell frequency was found to be characteristic of homozygotes of the low activity appropriated enzymes for 3 loci (HFE187, GSTM1 and MTHFR) at least. This tendency was statistically significant in case of deletion polymorphism of the GSTM1 gene: TCR-mutant cell frequency of the homozygous carriers of a deletion at the GSTM1 locus was (4.63 +/- 0.18) x 10(-4) while it was (4.05 +/- 0.15) x 10(-4) in other groups of persons. The greatest mutant cell frequency was observed in carriers of the minor allele 4889G of the locus CYP1A. More often the increased values of the TCR-mutant cells (outside range "3sigma") were determined in women with genotypes A/G or G/G of the locus CYP1A1 (25%) than in carries of the normal genotype A/A (1.6%) (OR = 20.6; p = 0.0002). The comparison of the groups of women with reproductive system diseases reveals significant elevation in the mean TCR-mutant cell frequency in inhabitants of the most radiation polluted region among others.
Zamulaeva
I A
IA
Sal'nikova
L E
LE
Ivanova
T I
TI
Orlova
N V
NV
Smirnova
S G
SG
Lapteva
N Sh
NSh
Chumachenko
A G
AG
Belopol'skaia
O B
OB
Krikunova
L I
LI
Smirnova
I A
IA
Saenko
A S
AS
Rubanovich
A V
AV
D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004247:DNA / Q000235:genetics; D004781:Environmental Exposure; D005260:Female; D005434:Flow Cytometry; D005831:Genital Diseases, Female / Q000235:genetics / Q000276:immunology; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation; D011110:Polymorphism, Genetic; D011848:Radioactive Pollutants; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012426:Russia
C103673:HFE protein, human; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D011848:Radioactive Pollutants; D011948:Receptors, Antigen, T-Cell; D004247:DNA; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase