[Apoptosis of U937 cell line promoted by matrine through MAPK signal transduction pathway].
OBJECTIVE
To study the anti-cancer effect of matrine (Mat) on U937 cell line and its possible molecular mechanism.


METHODS
The cells were cultured in medium containing either 0.1, 0.2, 0.3, 0.4, or 0.5 g x L(-1) of Mat. The morphological alteration was observed by inverted microscopy and electron microscopy. Cell proliferation was analyzed by Try pan blue staining and MTT. The method of Western Blot was used to detect phosphorylation activity of MAPK.


RESULTS
Matrine had a significant inhibitory effect on proliferation of U937 cell line at the concentration of 0.2 g x L(-1). Treated with matrine of 0.2 g x L(-1) for 48 h, U937 cells became smaller and appeared more round than previously. The number of U937 cells showing apoptosis increased with elevation of the concentration of the matrine. Matrine had an ability of inhibiting the activity of ERK and increasing the activities of p38 and JNK to some degree in U937 cells.


CONCLUSIONS
Matrine can inhibit the proliferation of U937 cell line in vitro and induce its apoptosis possibly through inhibiting the activity of ERK and increasing the activities of p38 and JNK in U937 cells.
The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. zesongyang@126.com
Zesong
Z
Yang
Jun
J
Mu
Jianbin
J
Chen
Qunfang
Q
Ge
Yang
Y
Liao
Qianwei
Q
Lu
Zonggan
Z
Huang
D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D011807:Quinolizines / Q000494:pharmacology*; D020298:U937 Cells; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D000093842:Matrines