Preparation of (14)C-labeled multiwalled carbon nanotubes for biodistribution investigations.
A new method allowing the (14)C-labeling of carboxylic acid functions of carbon nanotubes is described. The key step of the labeling process is a decarbonylation reaction that has been developed and optimized with the help of a screening method. The optimized process has been successfully applied to multiwalled carbon nanotubes (MWNTs), and the corresponding (14)C-labeled nanotubes were used to investigate their in vivo behavior. Preliminary results obtained after i.v. contamination of rats revealed liver as the main target organ. Radiolabeling of NTs with a long-life radioactive nucleus like (14)C, coupled to a highly sensitive autoradiographic method, that provides a unique detection threshold, will make it possible to determine for a long time period whether or not NTs remain in any organs after animal exposure.
Georgin
Dominique
D
CEA, IBITECS, SCBM and SIMOPRO, CEA, IRAMIS, SPAM and SCM, Gif sur Yvette, F-91191, France, and CEA, IRCM, SREIT, Bruyeres le Chatel, F-91680, France.
Czarny
Bertrand
B
Botquin
Magali
M
Mayne-L'hermite
Martine
M
Pinault
Mathieu
M
Bouchet-Fabre
Brigitte
B
Carriere
Marie
M
Poncy
Jean-Luc
JL
Chau
Quang
Q
Maximilien
Rémy
R
Dive
Vincent
V
Taran
Frédéric
F
D000818:Animals; D002250:Carbon Radioisotopes / Q000737:chemistry; D007267:Injections; D007553:Isotope Labeling; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009570:Nitriles / Q000737:chemistry; D051381:Rats; D014018:Tissue Distribution
D002250:Carbon Radioisotopes; D037742:Nanotubes, Carbon; D009570:Nitriles