Quantum dots for labeling adipose tissue-derived stem cells.
Adipose tissue-derived stem cells (ASCs) have a self-renewing ability and can be induced to differentiate into various types of mesenchymal tissue. Because of their potential for clinical application, it has become desirable to label the cells for tracing transplanted cells and for in vivo imaging. Quantum dots (QDs) are novel inorganic probes that consist of CdSe/ZnS-core/shell semiconductor nanocrystals and have recently been explored as fluorescent probes for stem cell labeling. In this study, negatively charged QDs655 were applied for ASCs labeling, with the cationic liposome, Lipofectamine. The cytotoxicity of QDs655-Lipofectamine was assessed for ASCs. Although some cytotoxicity was observed in ASCs transfected with more than 2.0 nM of QDs655, none was observed with less than 0.8 nM. To evaluate the time dependency, the fluorescent intensity with QDs655 was observed until 24 h after transfection. The fluorescent intensity gradually increased until 2 h at the concentrations of 0.2 and 0.4 nM, while the intensity increased until 4 h at 0.8 nM. The ASCs were differentiated into both adipogenic and osteogenic cells with red fluorescence after transfection with QDs655, thus suggesting that the cells retain their potential for differentiation even after transfected with QDs655. These data suggest that QDs could be utilized for the labeling of ASCs.
Yukawa
Hiroshi
H
Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461-0047, Japan. hiroshiy@med.nagoya-u.ac.jp
Mizufune
Shogo
S
Mamori
Chiharu
C
Kagami
Yukimasa
Y
Oishi
Koichi
K
Kaji
Noritada
N
Okamoto
Yukihiro
Y
Takeshi
Manabu
M
Noguchi
Hirofumi
H
Baba
Yoshinobu
Y
Hamaguchi
Michinari
M
Hamajima
Nobuyuki
N
Hayashi
Shuji
S
D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis; D045663:Quantum Dots; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors
D005456:Fluorescent Dyes; D008081:Liposomes