Combination antioxidant effect of α-tocoferol and erdosteine in ischemia-reperfusion injury in rat model.
BACKGROUND
Renal ischemia/reperfusion (I/R) which is an important cause of renal dysfunction is inevitable in renal transplantation, surgical revascularization of the renal artery, partial nephrectomy and treatment of suprarenal aortic aneurysms.


OBJECTIVE
The purpose of this study was to investigate the efficacy of α-tocopherol and erdosteine combination in the reduction in injury induced by ROS in a rat model of renal ischemia-reperfusion.


METHODS
Thirty-six- male Wistar albino rats weighing 200-250 g were utilized for this study. Rats were divided into six groups, and each group was consistent of six rats: (1) sham-operated (control), (2) ischemia group (3) I/R group, (4) I/R/α-tocoferol group (5) I/erdosteine group (6). I/R/α-tocoferol and erdosteine group. Biochemically tissue MDA, XO and SOD activities, light and electron microscopic findings were evaluated.


RESULTS
The erdosteine and α-tocoferol significantly reversed the effect of protein oxidation and lipid peroxidation induced by I/R shown by the decreased levels of MDA and XO activities. Both MDA and XO levels were found to be lower in group 6 compared to single agent treatment groups, and this was significantly different. All treatment groups showed increased SOD activity, which accounts for their oxidative properties. The mean Paller score of the combination treatment group (group 6) was lower than all groups except the sham group (3.67 ± 1.2), and this finding was statistically significant (0.05). Our results showed that the antioxidant pretreatment with α-tocopherol and erdosteine combination reduced lipid peroxidation of renal cellular membranes in a model of normothermic renal ischemia-reperfusion in rats. Combination of erdosteine and α-tocopherol has a synergistic effect of protection against oxidative processes. Long-term use of α-tocopherol seems to have a greater effect on the prevention of IR injury. However, further investigations are needed for the clinical applications of our findings.
Yurdakul
Talat
T
Department of Urology, Selcuk University Meram Medical Faculty, Konya, Turkey.
Kulaksizoglu
Haluk
H
Pişkin
Mehmet Mesut
MM
Avunduk
Mustafa Cihat
MC
Ertemli
Esra
E
Gokçe
Gürhan
G
Barişkaner
Hülagü
H
Byükbaş
Sadik
S
Kocabas
Volkan
V
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013864:Thioglycolates / Q000008:administration & dosage*; D013876:Thiophenes / Q000008:administration & dosage*; D014969:Xanthine Oxidase / Q000378:metabolism; D024502:alpha-Tocopherol / Q000008:administration & dosage*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D013864:Thioglycolates; D013876:Thiophenes; D008315:Malondialdehyde; C048498:erdosteine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D024502:alpha-Tocopherol