Synthesis of rapeseed biodiesel using short-chained alkyl acetates as acyl acceptor.
In this study, we conducted experiments using a response surface methodology to determine the optimal reaction conditions for the enzymatic synthesis of biodiesel from rapeseed oil and short-chained alkyl acetates, such as methyl acetate or ethyl acetate, as the acyl acceptor at 40 degrees C. Based on our response surface methodology experiments, the optimal reaction conditions for the synthesis of biodiesel were as follows: methyl acetate as acyl acceptor, catalyst concentration of 16.50%, oil-to-methyl acetate molar ratio of 1:12.44, and reaction time of 19.70 h; ethyl acetate as acyl acceptor, catalyst concentration of 16.95%, oil-to-ethyl acetate molar ratio of 1:12.56, and reaction time of 19.73 h. The fatty acid ester content under the above conditions when methyl acetate and ethyl acetate were used as the acyl acceptor was 58.0% and 62.6%, respectively. The statistical method described in this study can be applied to effectively optimize the enzymatic conditions required for biodiesel production with short-chained alkyl acetates.
Department of Biotechnology, Pukyong National University, Busan 608-737, Korea.
Gwi-Taek
GT
Jeong
Don-Hee
DH
Park
D000085:Acetates / Q000737:chemistry*; D055162:Biocatalysis; D056804:Biofuels; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D029741:Brassica rapa / Q000737:chemistry / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry; D008049:Lipase / Q000378:metabolism
D000085:Acetates; D056804:Biofuels; D005227:Fatty Acids; D008049:Lipase