Impact of RGD micro-patterns on cell adhesion.
In order to avoid the problems related to biomaterial use (inflammation, infections, aseptic loosening, etc.), a new approach consisting of associating the material and autologous cells before implantation is being developed, thus requiring a perfect cooperation between the material's surface and the cell. To improve cell adhesion to biomaterials, a suitable method is to functionalize their surface by pro-adhesive ligand grafting. The aim of this study was to covalently graft RGD containing peptides onto a poly-(ethylene terephthalate) surface in well-defined microstructures in order to control MC3T3 cell adhesion. We followed two different routes for obtaining micro-patterned materials: (1) a photoablation technique using an excimer laser and (2) a photolithography process. The resulting patterns were characterized by optical microscopy, scanning electron microscopy, optical profilometry and high resolution mu-imager. The biological evaluation of cell adhesion onto the micro-patterned surfaces was carried out using optical microscopy, scanning electron microscopy and fluorescence microscopy. Cells seeded onto photolithographical or photoablated micro-patterned PET exhibited an alignment with the RGD domains and appear to be connecting through pseudopods extending towards each other. Whatever the technique used to create micro-patterns, a cell alignment occurs once the thickness of the RGD line reaches approximately 100 microm. These results prove the importance of microstructured surfaces for the elaboration of tissue engineered biomaterials.
Chollet
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C
INSERM, U577, Bordeaux F-33076, Univ. Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France. celine.chollet@inserm.fr
Lazare
S
S
Guillemot
F
F
Durrieu
M C
MC
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D054018:Lasers, Excimer; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid