[Local X-irradiation promotes regeneration of spinal cord central nervous system in a rat model].
OBJECTIVE
To evaluate the effect of local X-irradiation on spinal cord injury by using physiology, kinology, electrophysiology and histology method.


METHODS
46 female Sprague-Dawley rats were subjected to spinal cord injury by weight dropping on T(11-12). All animals were divided into 3 groups randomly. One of the animal groups was irradiated with 10 Gy at the lesion site; another was irradiated with 20 Gy, the other without irradiation is regarded as sham-group. The animals were euthanized at different time points at 4 and 12 weeks after irradiation. Spinal cord callus was assessed by using physiology, kinology, and electrophysiology and histology method.


RESULTS
In all the groups, the NF at 14 weeks were found higher than that of 6 weeks. Both 10 Gy irradiated and 20 Gy irradiated groups were higher than those of group at each time point (P < 0.05). The MBP decreased at 14 weeks in irradiated groups (P < 0.05), but increased at 14 weeks in sham-group (P < 0.05), the MBP of irradiated groups was lower than that sham-group at 14 weeks (P < 0.05). The GFAP and Nogo-A at 14 weeks were higher than that in 6 weeks in all the groups (P < 0.05), and there was no statistical significance with physiology, kinology, electrophysiology test in all groups.


CONCLUSIONS
A self-repair mechanism exists after SCI, which will last at least 14 weeks. Local irradiation promotes the regeneration of spinal cord system after injury to some extent.
Shen
Yi-Xin
YX
Department of Orthopaedics, the Second Affiliated Hospital of Suzhou University, Suzhou, China. ShenYX@mail.szldbz.gov.cn
Liu
Dong
D
Sun
Yong-Ming
YM
Zhou
Xiao-Zhong
XZ
Tian
Ye
Y
Lu
Zheng-Feng
ZF
Fan
Zhi-Hai
ZH
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects*; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000532:radiotherapy*; D014965:X-Rays