In vitro evaluation of feline leukocytes radiolabeled in whole blood with 99mTc stannous colloid.
Technetium-99m stannous colloid (9mTcSnC) has been used to radiolabel human leukocytes to investigate various inflammatory disorders. We investigated the in vitro behavior of feline leukocytes labeled in whole blood with 99mTcSnC. Heparinized blood samples were collected from healthy cats and divided into control and test aliquots. The latter were labeled with 99mTcSnC using a standard procedure. Leukocyte viability was determined for each sample using a trypan blue exclusion test. Labeling efficiency was determined for test aliquots. Test aliquots were layered onto Histopaque-1077 and centrifuged before measurement of radioactivity of the blood components. Leukocytes from radiolabeled and control samples were washed and incubated with opsonized zymosan particles to allow assessment of phagocytic function. Aliquots were taken from radiolabeled feline leukocyte samples at 1, 3, 4, and 7 h postlabelling. After centrifugation of each aliquot, radioactivity of the supernatant and pellet was measured and the labeling retention determined. Leukocyte viability in both radiolabeled and control samples was > 98%. The labeling efficiency was 95.2 +/- 0.14%. The distribution of radioactivity in feline blood was found to be 3.4 +/- 0.18% in plasma, 39.0 +/- 0.37% in erythrocytes, and 57.6 +/- 0.38% in leukocytes. Labeled feline leukocytes had phagocytic activity of 90.9 +/- 0.18% (control 91.3 +/- 0.15%). The radiolabeled leukocytes retained 93.4 +/- 0.19% of the radioactivity up to 7h postlabeling. 99TcSnC efficiently labeled feline leukocytes with no effect on viability and minimal effect on phagocytic function. The percentage retention of radioactivity by the leukocytes was still high at 7h postlabeling.
Abushhiwa
Mohamed H
MH
Faculty of Veterinary Science, The University of Melbourne, Werribee, Vic. 3030, Australia. m.abushhiwa@pgrad.unimelb.edu.au
Salehi
Nouria S
NS
Whitton
Robert C
RC
Charles
Jennifer A
JA
Finnin
Peter J
PJ
Lording
Peter M
PM
Parry
Bruce W
BW
D000818:Animals; D002415:Cats / Q000097:blood*; D002470:Cell Survival; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000662:veterinary*; D007962:Leukocytes / Q000502:physiology; D010587:Phagocytosis; D019275:Radiopharmaceuticals; D017556:Technetium Compounds / Q000097:blood*; D017971:Tin Compounds / Q000097:blood*
D019275:Radiopharmaceuticals; D017556:Technetium Compounds; D017971:Tin Compounds; C033493:technetium Tc 99m tin colloid