Acute pancreatitis, bacterial translocation, and different octreotide regimens: an experimental study.
OBJECTIVE
To determine the effect of octreotide, octreotide with zinc, levamisole, and misoprostol on the bacterial translocation that develops in rats with acute pancreatitis (AP).


METHODS
A total of 36 rats were divided into six groups, each consisting of six rats. Only laparotomy was performed on the first group. Acute pancreatitis was performed on the second group. Octreotide was given to the third, fourth, fifth, and sixth groups. Octreotide, octreotide with zinc, levamisole, and misoprostol were given to groups III, IV, V, VI, respectively. Rats were euthanized 48 h after the occurrence of AP. Blood and mesenteric lymph node samples were collected for polymerase chain reaction (PCR). Pancreatic tissue and terminal ileum were obtained for histopathological examinations.


RESULTS
The severity of pancreatitis and mucosal damage of the terminal ileum was higher in group II than groups I, III, IV, V, and VI, histopathologically (P < 0.05). There wasn't a significant difference with respect to OA with Zn or L or M and OA group (P > 0.05). A significant difference was found in PCR positivity in blood and mesenteric lymph node between groups I and II (P < 0.05).


CONCLUSIONS
In AP, administering octreotide alone significantly prevented the bacterial translocation by preventing mucosal damage. The zinc, levamisole, or misoprostol with octreotide did not influence the results.
Department of General Surgery, Ankara Training and Research Hospital, Ankara 06340, Turkey.
Osman
O
Güler
Saadet
S
Akturan
Erol
E
Kisli
Iştar
I
Dolapçi
Muzaffer
M
Caydere
Atilla
A
Akova
D000208:Acute Disease; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D004359:Drug Therapy, Combination; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D007082:Ileum / Q000187:drug effects / Q000473:pathology; D007978:Levamisole / Q000494:pharmacology; D008297:Male; D016595:Misoprostol / Q000494:pharmacology; D015282:Octreotide / Q000494:pharmacology*; D010195:Pancreatitis / Q000097:blood / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D015032:Zinc / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D005765:Gastrointestinal Agents; D016595:Misoprostol; D007978:Levamisole; D015032:Zinc; D015282:Octreotide