Fabrication of Nanoscale Bioarrays for the Study of Cytoskeletal Protein Binding Interactions Using Nanoimprint Lithography.
We describe a high throughput patterning process used to create arrays of molecular-scale features for the study of cytoskeletal protein binding interactions. The process uses a shadow-evaporated metal mask to facilitate lift-off of features defined by nanoimprint lithography. This simple and robust approach alleviates difficulties in pattern transfer of ultra-small features and results in arrays of highly ordered sub-10 nm features which are then functionalized with extracellular matrix proteins. Application of these arrays is demonstrated in cell spreading assays.
Schvartzman
M
M
Dept. of Chemical Engineering, Columbia University, New York, NY 10027, USA.
Nguyen
K
K
Palma
M
M
Abramson
J
J
Sable
J
J
Hone
J
J
Sheetz
M P
MP
Wind
S J
SJ