Liquid chromatography with complementary electrospray and inductively coupled plasma mass spectrometric detection of ferrocene-labelled peptides and proteins.
Succinimidylferrocenyl propionate (SFP) is introduced as labelling agent for amino functions in peptides and proteins. The resulting derivatives are characterised by considerably lower polarity compared with the native analytes and can thus be well separated by means of reversed phase liquid chromatography (RP-LC). The reaction products are characterised by electrospray ionisation mass spectrometry (ESI-MS) and inductively coupled plasma mass spectrometry (ICP-MS). A further advantage of the method is a simple and straightforward derivatisation protocol. Different basic and acidic model proteins as lysozyme, beta-lactoglobulin A and insulin were derivatised using SFP. Furthermore, the first dual-labelling strategy of thiol and amino groups with ferrocene-based reagents is presented. Whereas the amino groups were derivatised with SFP, the thiol groups were functionalised by reaction with ferrocenecarboxylic acid(2-maleimidoyl)ethylamide. Again, LC/ESI-MS is a suitable tool to characterise the modified peptides and proteins.
Bomke
Susanne
S
Westfälische Wilhelms-Universität Münster, Institut für Anorganische und Analytische Chemie, Corrensstrasse 30, 48149 Münster, Germany.
Pfeifer
Thorben
T
Meermann
Björn
B
Buscher
Wolfgang
W
Karst
Uwe
U
D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005296:Ferrous Compounds / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D000075163:Metallocenes; D009113:Muramidase / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013194:Staining and Labeling / Q000379:methods*
D005296:Ferrous Compounds; D007328:Insulin; D007782:Lactoglobulins; D000075163:Metallocenes; D010455:Peptides; D009113:Muramidase; C004998:ferrocene