Binding fullerenol C(60)(OH)(24) to dsDNA.
The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported. The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1). Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.
Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica, 700487 Iasi, Romania.
Mariana
M
Pinteala
Andrei
A
Dascalu
Cezar
C
Ungurenasu
D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D037741:Fullerenes / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004337:Drug Carriers; D037741:Fullerenes; D046911:Macromolecular Substances; C408042:fullerol C60(OH)24; D004247:DNA