Impact of RGD micro-patterns on cell adhesion.
In order to avoid the problems related to biomaterial use (inflammation, infections, aseptic loosening, etc.), a new approach consisting of associating the material and autologous cells before implantation is being developed, thus requiring a perfect cooperation between the material's surface and the cell. To improve cell adhesion to biomaterials, a suitable method is to functionalize their surface by pro-adhesive ligand grafting. The aim of this study was to covalently graft RGD containing peptides onto a poly-(ethylene terephthalate) surface in well-defined microstructures in order to control MC3T3 cell adhesion. We followed two different routes for obtaining micro-patterned materials: (1) a photoablation technique using an excimer laser and (2) a photolithography process. The resulting patterns were characterized by optical microscopy, scanning electron microscopy, optical profilometry and high resolution mu-imager. The biological evaluation of cell adhesion onto the micro-patterned surfaces was carried out using optical microscopy, scanning electron microscopy and fluorescence microscopy. Cells seeded onto photolithographical or photoablated micro-patterned PET exhibited an alignment with the RGD domains and appear to be connecting through pseudopods extending towards each other. Whatever the technique used to create micro-patterns, a cell alignment occurs once the thickness of the RGD line reaches approximately 100 microm. These results prove the importance of microstructured surfaces for the elaboration of tissue engineered biomaterials.
INSERM, U577, Bordeaux F-33076, Univ. Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France. celine.chollet@inserm.fr
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D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D054018:Lasers, Excimer; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid