DkMyb4 is a Myb transcription factor involved in proanthocyanidin biosynthesis in persimmon fruit.
Proanthocyanidins (PAs) are secondary metabolites that contribute to the protection of the plant and also to the taste of the fruit, mainly through astringency. Persimmon (Diospyros kaki) is unique in being able to accumulate abundant PAs in the fruit flesh. Fruits of the nonastringent (NA)-type mutants lose their ability to produce PA at an early stage of fruit development, while those of the normal astringent (A) type remain rich in PA until fully ripened. The expression of many PA pathway genes was coincidentally terminated in the NA type at an early stage of fruit development. The five genes encoding the Myb transcription factor were isolated from an A-type cultivar (Kuramitsu). One of them, DkMyb4, showed an expression pattern synchronous to that of the PA pathway genes in A- and NA-type fruit flesh. The ectopic expression of DkMyb4 in kiwifruit (Actinidia deliciosa) induced PA biosynthesis but not anthocyanin biosynthesis. The suppression of DkMyb4 in persimmon calluses caused a substantial down-regulation of the PA pathway genes and PA biosynthesis. Furthermore, analysis of the DNA-binding ability of DkMyb4 showed that it directly binds to the MYBCORE cis-motif in the promoters of the some PA pathway genes. All our results indicate that DkMyb4 acts as a regulator of PA biosynthesis in persimmon and, therefore, suggest that the reduction in the DkMyb4 expression causes the NA-type-specific down-regulation of PA biosynthesis and resultant NA trait.
Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Takashi
T
Akagi
Ayako
A
Ikegami
Tomoyuki
T
Tsujimoto
Shozo
S
Kobayashi
Akihiko
A
Sato
Atsushi
A
Kono
Keizo
K
Yonemori
D029042:Actinidia / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D029788:Diospyros / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D044945:Proanthocyanidins / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000378:metabolism*; D016372:RNA, Antisense / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014170:Transformation, Genetic
D010940:Plant Proteins; D044945:Proanthocyanidins; D020598:Proto-Oncogene Proteins c-myb; D016372:RNA, Antisense; D014157:Transcription Factors