Localization of epididymal protease inhibitor in adult rat and its transcription profile in testis during postnatal development.
To investigate the expression pattern of rat Eppin (epididymal protease inhibitor; official symbol Spinlw1), we detected mRNA transcripts and subsequent protein translation of Eppin in several sorts of tissues by RT-PCR and western blotting. Then immunohistochemistry was performed for more detailed observation. The testicular transcription level was monitored by real-time PCR throughout postnatal development. We found that rat Eppin was specifically expressed in the testis and epididymis. The testicular transcription was slight in neonatal (1-day) and infantile stages (5-, 7- and 10-day). It increased sharply thereafter, with maximum expression level (about 38-fold compared with that of 1-day old rat) detected in prepubertal stage (15-day). Then a slightly declined but stable level (about 20-fold compared with that of 1-day old rat) was kept in pubertal-early adult (30-day) and adult (60-day) stages of postnatal maturation. In the adult rat, EPPIN protein was mainly localized in the elongated spermatids and epididymal epithelial cells. Sperm in the epididymal duct were all covered with EPPIN and its level kept constant during incubation under conditions used to achieve capacitation. Its stage-specific expression in the testis suggests that EPPIN may be important during spermatogenesis especially for the spermatid elongation. The abundant production of epididymal EPPIN indicated indirectly that it might play a role in the function of the epididymis.
Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China.
Zeng-Hui
ZH
Bian
Jie
J
Zhang
Xin-Liang
XL
Ding
Bin
B
Zhang
Zeng-Jun
ZJ
Wang
Chun-Cheng
CC
Lu
Lin
L
Song
Shou-Lin
SL
Wang
Xin-Ru
XR
Wang
D000818:Animals; D004822:Epididymis / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D011480:Protease Inhibitors / Q000032:analysis*; D053491:Proteinase Inhibitory Proteins, Secretory / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000502:physiology; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
D011480:Protease Inhibitors; D053491:Proteinase Inhibitory Proteins, Secretory; C546856:eppin protein, rat