Three-dimensional alginate spheroid culture system of murine osteosarcoma.
Osteosarcoma (OS) is the most common primary malignant tumor of the bone and often forms pulmonary metastases, which are the most important prognostic factor. For further elucidation of the mechanism underlying the progression and metastasis of human OS, a culture system mimicking the microenvironment of the tumor in vivo is needed. We report a novel three-dimensional (3D) alginate spheroid culture system of murine osteosarcoma. Two different metastatic clones, the parental Dunn and its derivative line LM8, which has a higher metastatic potential to the lungs, were encapsulated in alginate beads to develop the 3D culture system. The beads containing murine OS cells were also transplanted into mice to determine their metastatic potential in vivo. In this culture system, murine OS cells encapsulated in alginate beads were able to grow in a 3D structure with cells detaching from the alginate environment. The number of detaching cells was higher in the LM8 cell line than the Dunn cell line. In the in vivo alginate bead transplantation model, the rate of pulmonary metastasis was higher with LM8 cells compared with that of Dunn cells. The cell characteristics and kinetics in this culture system closely reflect the original malignant potential of the cells in vivo.
Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, Mie 514-8507, Japan. k_akeda@clin.medic.mie-u.ac.jp
Koji
K
Akeda
Akinobu
A
Nishimura
Haruhiko
H
Satonaka
Ken
K
Shintani
Katsuyuki
K
Kusuzaki
Akihiko
A
Matsumine
Yuichi
Y
Kasai
Koichi
K
Masuda
Atsumasa
A
Uchida
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003692:Delayed-Action Preparations / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008863:Microspheres; D012516:Osteosarcoma / Q000556:secondary*; D018874:Spheroids, Cellular / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000464:Alginates; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; D020723:Glucuronic Acid