Antifouling coatings for optoelectronic tweezers.
Optoelectronic tweezers enables parallel manipulation of individual single cells using optical addressing and optically induced dielectrophoretic force. This provides a useful platform for performing a variety of biological functions, such as cell manipulation, cell sorting, and cell electroporation. However, in order to obtain more reliable cellular manipulation, especially of adherent mammalian cells, antifouling coatings need to be used to avoid non-specific cell adherence. Two antifouling coatings are discussed here, which can reduce the amount of non-specific adherence by as much as a factor of 30.
Lau
Aldrich N K
AN
Applied Biosystems, Foster City, CA 94404, USA. lauan@appliedbiosystems.com
Ohta
Aaron T
AT
Phan
Huan L
HL
Hsu
Hsan-Yin
HY
Jamshidi
Arash
A
Chiou
Pei-Yu
PY
Wu
Ming C
MC
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003584:Cytological Techniques / Q000295:instrumentation*; D004581:Electronics; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D052898:Optical Tweezers; D011092:Polyethylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission
D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols