[Detection of metallo-beta-lactamase in Pseudomonas aeruginosa isolated from hospitalized patients in Goiânia, State of Goiás].
Pseudomonas aeruginosa is a bacterium frequently isolated from hospital environments. This study had the aims of evaluating the susceptibility profile of Pseudomonas aeruginosa previously isolated from patients in a hospital in Goiânia (Goiás, Brazil), performing phenotypic screening for metallo-beta-lactamase production and detecting its genes using the polymerase chain reaction technique. Seventy-five 75 Pseudomonas aeruginosa isolates were evaluated between January 2005 and January 2007. Biochemical identification was performed using the API 20E system and an antibiogram was produced using the Kirby-Bauer method. Among the 62 isolates that were resistant to imipenem and ceftazidime, 35 (56.4%) produced metallo-beta-lactamase, while 26 (74.3%) showed the bla(SPM-1) gene. The frequency of Pseudomonas aeruginosa that produces metallo-beta-lactamase suggests that greater control over the dissemination of resistance in hospital environments is needed.
Gonçalves
Diana Christina Pereira Santos
DC
Programa de Pós-graduação em Medicina Tropical e Saúde Publica, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás.
Lima
Ana Beatriz Mori
AB
Leão
Lara Stefania Netto de Oliveira
LS
Filho
José Rodrigues do Carmo
JR
Pimenta
Fabiana Cristina
FC
Vieira
José Daniel Gonçalves
JD
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D003428:Cross Infection / Q000382:microbiology; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases