[Effect of estradiol on fluctuation of calcium in swine granulose cells stimulated by theophylline and prolactin].
Effect of estradiol on Ca2+ exit from intracellular stores in swine granulose cells activated by theophylline and prolactin is investigated with using fluorescent Ca2+ sensitive probe chlortetracycline. It was shown that prolactin and theophylline stimulated Ca2+ exit from intracellular stores. In absence of estradiol if we treatment of oocytes by theophylline and prolactin together we have not found influence at Ca2+ exit from intracellular stores. In the presence of estradiol we had opposite results: we tested additional Ca2+ exit at common action of theophylline and prolactin. Protein kinase C influences on Ca2+ exit from intracellular stores during common affect of theophylline and prolactin only in the presence of estradiol. Protein kinase A does not influence this process. The obtained data indicate an influence of estradiol on Ca2+ exit from intracellular stores in swine granulose cells stimulated by joint action of prolactin and theophylline.
Denisenko
V Iu
VIu
Kuz'mina
T I
TI
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D011388:Prolactin / Q000494:pharmacology*; D013552:Swine; D013806:Theophylline / Q000494:pharmacology*
D004958:Estradiol; D011388:Prolactin; D013806:Theophylline; D002118:Calcium