The effect of linseed on intramuscular fat content and adipogenesis related genes in skeletal muscle of pigs.
The aim of the study was to investigate the effect of n-3 PUFA enrichment in longissimus muscle on intramuscular fat (IMF) content and expression of related genes in growing-finishing barrows. Two isoenergetic, isonitrogenous and isolipidic diets were formulated: one was basal diet and the other contained 10% linseed. Twenty-four Landrace x NewDamLine barrows weighing 35 +/- 3.7 kg were randomly assigned to four treatment groups with six pigs per group. During the whole experimental period of 90 days, all groups were first fed the basal diet and then the linseed diet for 0, 30, 60, and 90 days before slaughter, respectively. Meat quality, fatty acid composition, and expression of genes involved in adipogenesis in longissimus muscle were measured and analyzed. The IMF content increased linearly (P < 0.05) as the linseed diet feeding time prolonged. Meanwhile, n-3 PUFA content and expression of peroxisome proliferator-activated receptor delta (PPARdelta), PPARgamma, adipocyte fatty acid-binding protein (aP2) and lipoprotein lipase (LPL) increased linearly (P < 0.01) as well, while the expression of wingless related MMTV integration site 10b (Wnt10b) linearly decreased (P < 0.01). Furthermore, significant (P < 0.01) quadratic or linear relation was observed between n-3 PUFA enrichment and expression of these genes, while significant (P < 0.01) quadratic or linear relation was observed between the expression of PPARgamma, aP2 or Wnt10b and IMF content. These data show that enhancing n-3 PUFA enrichment in muscle leads to significant increase in IMF content. A possible explanation is due to alterations in the expression of genes involved in adipogenesis, however this will need to be confirmed by protein and enzyme activity studies.
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China. sixfires@126.com
He-Feng
HF
Luo
Hong-Kui
HK
Wei
Fei-Ruo
FR
Huang
Zheng
Z
Zhou
Si-Wen
SW
Jiang
Jian
J
Peng
D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D019597:Flax; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D047494:PPAR delta / Q000235:genetics* / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D013552:Swine
D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D047494:PPAR delta; D047495:PPAR gamma; D008071:Lipoprotein Lipase