Effects of temperature, ultraviolet radiation and pectin methyl esterase on aerobic methane release from plant material.
This study examines the effects of different irradiance types on aerobic methane (CH(4)) efflux rates from terrestrial plant material. Furthermore, the role of the enzyme pectin methyl esterase (PME) on CH(4) efflux potential was also examined. Different types of plant tissue and purified pectin were incubated in glass vials with different combinations of irradiation and/or temperature. Purified dry pectin was incubated in solution, and with or without PME. Before and after incubation, the concentration of CH(4) was measured with a gas chromatograph. Rates of CH(4) emission were found to depend exponentially on temperature and linearly on UV-B irradiance. UV-B had a greater stimulating effect than UV-A, while visible light had no effect on emission rates. PME was found to substantially reduce the potential for aerobic CH(4) emissions upon demethylation of pectin.
Bruhn
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D
Ecosystems Programme, Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark, Roskilde, Denmark. dabr@risoe.dtu.dk
Mikkelsen
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TN
Obro
J
J
Willats
W G T
WG
Ambus
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P
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis*; D010368:Pectins / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects; D010944:Plants / Q000378:metabolism* / Q000528:radiation effects*; D013696:Temperature; D014466:Ultraviolet Rays
D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D008697:Methane