Detection and genotyping of human papillomavirus DNA using polymerase chain reaction short PCR fragment 10-line probe assay in abnormal Papanicolaou-stained cervicovaginal smears.
OBJECTIVE
To evaluate the effectiveness of the short PCR fragment 10-line probe assay (SPF10-LiPA) system in detection of human papillomavirus (HPV) in abnormal Papanicolaou-stained cervicovaginal smears.


METHODS
We included 20 abnormal Papanicolaou-stained cervicovaginal smears. Samples were tested for HPV DNA detection and genotyping using the SPF10-LiPA system.


RESULTS
All samples amplified the INF150 gene control. In 2 of 20 cases, amplification of the viral sequences was negative. Of processed samples, 18 of 20 presented HPV infection; of them, 56% showed only 1 type of HPV infection; the remaining presented > or = 2 types of HPV (multiple infections). HPV16 was the most frequent specific viral in 60%, in single and multiple infections, followed by HPV18 (20%), HPV6 (10%) and HPV58 (10%). We also found HR-HPV45, 52, 58 and 68 and LR-HPV6, 11 and 70 viral DNA types. These multiple infections were detected with greater frequencies in atypical squamous cells of undetermined significance and in the low-grade squamous intraepithelial lesions.


CONCLUSIONS
The SPF10-LiPA system is efficient, sensitive, fast and simple for detecting and genotyping HPV DNA in abnormal Papanicolaou-stained cervicovaginal smears, which could be enormously useful in routine investigation for better clinical management of the patient.
Department of Clinic Bioanalysis, University of Los Andes, Mérida, Venezuela. tmorelva@ula.ve
Morelva Toro
MT
de Méndez
Antonio Llombart
AL
Bosch
D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology*; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D065006:Papanicolaou Test; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*
D004279:DNA, Viral