[The role of cholesterol in exo- and endocytosis of the synaptic vesicles at the frog motor nerve terminal].
In experiments on the frog neuro-muscular preparations using electrophysiological (two electrode fixing of potential) and optical (fluorescent endocytic dye FM1-43) methods, the value of surface cholestertol for exo-endocytic cycle of synaptic vesicles at the prolonged rhythmic activity (20 Hz--3 minutes) was investigated. It is shown that extraction of cholesterol from surface membranes by methyl-betta-cyclodextrin (1 mM MCD) leads to the expressed shifts in recycling of synaptic vesicles. Exocytosis of vesicles is decreased, and oppression of processes leading to restoration of the number of vesicles of ready releasable pool is observed. Cholesterol replacement from external membranes and membranes of recycling synaptic vesicles in addition to above described effects breaks processes of endocytosis and recycle of synaptic vesicles. Thus, in the processes of exocytosis, the key role is played by cholesterol of plasmatic membranes, and endocytosis critically depends on the amount of cholesterol in the membranes of synaptic vesicles.
Petrov
A M
AM
kasimov
M R
MR
Giniatullin
A R
AR
Tarakanova
O I
OI
Zefirov
A L
AL
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000494:pharmacology / Q000502:physiology*; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D009469:Neuromuscular Junction / Q000502:physiology*; D011895:Rana ridibunda; D013572:Synaptic Vesicles / Q000187:drug effects / Q000502:physiology*
D002784:Cholesterol