Purification of Arabidopsis argonaute complexes and associated small RNAs.
Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.
National Institute of Biological Sciences, Beijing, China.
Yijun
Y
Qi
Shijun
S
Mi
D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D060565:Argonaute Proteins; D015153:Blotting, Western; D019175:DNA Methylation; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000737:chemistry / Q000302:isolation & purification*
C117457:AGO1 protein, Arabidopsis; C471209:AGO4 protein, Arabidopsis; C521197:AGO6 protein, Arabidopsis; D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; D034741:RNA, Small Interfering; D034743:RNA-Induced Silencing Complex