A successful pregnancy and live birth after intracytoplasmic sperm injection with globozoospermic sperm and electrical oocyte activation.
OBJECTIVE
To present the effectiveness of intracytoplasmic sperm injection (ICSI) using globozoospermic sperm and assisted oocyte activation by electrical stimulation.


METHODS
A case report.


METHODS
A private IVF center in Japan.


METHODS
A man with globozoospermia.


METHODS
Acridine orange (AO) test, mouse oocyte activation test, and ICSI with electrical oocyte activation.


METHODS
Fertilization, pregnancy, and live birth.


RESULTS
In the first ICSI attempt, neither of the two injected oocytes fertilized. Staining of the patient's sperm with AO showed that only 2.9% of the sperm emitted a green fluorescence at the characteristic round head (sperm with native DNA content). The mouse oocyte activation test using the roundheaded sperm showed that the normal fertilization rate was 78.9% when SrCl(2) was used for assisted oocyte activation; however, it was 6.0% without assisted oocyte activation. We confirmed that the sperm had defective ability to activate oocytes. In the second ICSI attempt, human oocytes were activated electrically with use of a single square direct current pulse after microinjection. All the seven injected oocytes fertilized normally, and two eight-cell embryos were transferred on day 3. Clinical pregnancy was confirmed, and a healthy girl weighing 2362 g was delivered at 37 weeks of gestation by cesarean section.


CONCLUSIONS
This is the first successful outcome of ICSI using globozoospermic sperm and electrical oocyte activation. The electroactivation obviates the need for the use of potentially harmful drugs for activation.
Egashira
Akiyoshi
A
Kuramoto Women's Clinic, Fukuoka, Japan.
Murakami
Masao
M
Haigo
Kosuke
K
Horiuchi
Toshitaka
T
Kuramoto
Takeshi
T
D000328:Adult; D000818:Animals; D004558:Electric Stimulation; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000002:abnormalities*