Adipokine gene transcription level in adipose tissue of runt piglets.
Runt piglets were used as a model for neonatal stress to test the hypothesis that stress during the pre-weaning period can alter adipokine gene transcription levels. Runts were selected by birth mass <1kg and compared to littermates (controls) of mean litter weight. Subcutaneous (SQ) and perirenal (PR) adipose tissues were collected at d1 (n=5), d7 (n=7) or d21 (n=9) of age. Real time PCR was used to quantify mRNA abundance for: leptin, adiponectin, interleukin 1beta (IL1beta), IL6, IL8, IL10, IL15, tumor necrosis factor alpha, haptoglobin, macrophage migration inhibitory factor (MIF), monocyte chemotactic protein, vascular endothelial growth factor and cyclophilin. Leptin and adiponectin mRNA abundance were lower, while IL1beta, IL6, IL10 and MIF mRNA abundance were higher in SQ of runts than controls at d1 (P<0.05). Leptin, IL6, IL10, haptoglobin and MIF mRNA abundance were higher in PR from runts than controls at d7 (P<0.05) and MIF mRNA abundance was elevated by 30 fold in PR of runts at d21 (P<0.001). Thus, stressors affecting neonatal runts produce different responses in adipokine gene transcription by PR and SQ than in normal sized littermates.
Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, USDA/ARS, Beltsville Agricultural Research Center, USDA, Beltsville, MD 20705, USA. timothy.ramsay@ars.usda.gov
T G
TG
Ramsay
M J
MJ
Stoll
T J
TJ
Caperna
D054392:Adipokines / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000235:genetics / Q000502:physiology; D001835:Body Weight / Q000235:genetics; D005260:Female; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D013552:Swine / Q000235:genetics*; D014158:Transcription, Genetic; D014886:Weaning
D054392:Adipokines; D012333:RNA, Messenger