The effects of the electric fields on hydrogels to achieve antitumoral drug release.
The achievement of electrochemotherapy was obtained using electrodes covered with guar gum (GG) hydrogel swollen in a sulfate bleomycin solution. The bleomycin delivery into the plasma membranes of cancer cells occurs only when field strength (V/cm) was applied, decreasing the drug contact with healthy tissues. The effect of the delivered bleomycin at different concentrations was examined on tumoral mouse fibroblast (NIH3T3) and human coronary artery endothelial cells. The GG hydrogel released the drug only when the field strength was applied and the amount depended on the electromotive force. Consequently, cellular survival depended on the field strength. Moreover in vitro results showed a bigger cellular mortality of the NIH3T3 compared with endothelial cells. The best parameters to be utilized in electrochemotherapy were ascertained.
Fantozzi
Federica
F
C.R.I.S.M.A., University of Siena, Via Aldo Moro 2, 53100 Siena, Italy. federica-fantozzi@libero.it
Arturoni
Enrico
E
Barbucci
Rolando
R
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D001761:Bleomycin / Q000737:chemistry*; D016923:Cell Death; D002470:Cell Survival; D018920:Coculture Techniques; D004560:Electricity; D053672:Electrochemotherapy; D004566:Electrodes; D042783:Endothelial Cells / Q000166:cytology; D005685:Galactans / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry; D013058:Mass Spectrometry; D051379:Mice; D041681:NIH 3T3 Cells; D053149:Plant Gums / Q000737:chemistry
D000970:Antineoplastic Agents; D005685:Galactans; D020100:Hydrogels; D008351:Mannans; D053149:Plant Gums; D001761:Bleomycin; C007894:guar gum