Effect of rosmarinic acid in motor dysfunction and life span in a mouse model of familial amyotrophic lateral sclerosis.
Amyotrophic lateral sclerosis (ALS) is a late-onset progressive neurodegenerative disease affecting motor neurons. About 2% of patients with the disease are associated with mutations in the gene encoding Cu/Zn superoxide dismutase (SOD1). The purpose of this study is to assess the effect of rosemary extract and its major constituents, rosmarinic acid (RA) and carnosic acid (CA), in human SOD1 G93A transgenic mice, which are well-established mouse models for ALS. The present study demonstrates that intraperitoneal administration of rosemary extract or RA from the presymptomatic stage significantly delayed motor dysfunction in paw grip endurance tests, attenuated the degeneration of motor neurons, and extended the life span of ALS model mice. In addition, RA administration significantly improved the clinical score and suppressed body weight loss compared with a vehicle-treated group. In conclusion, this study provides the first report that rosemary extract and, especially, RA have preventive effects in the mouse model of ALS.
Beauty Care Product Developing Team, Research and Development Center, Nagase & Co., Ltd., Hyogo, Japan.
Yosuke
Y
Shimojo
Kunio
K
Kosaka
Yoshihiro
Y
Noda
Takahiko
T
Shimizu
Takuji
T
Shirasawa
D000375:Aging / Q000187:drug effects; D000690:Amyotrophic Lateral Sclerosis / Q000150:complications* / Q000235:genetics / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects / Q000235:genetics; D002934:Cinnamates / Q000627:therapeutic use*; D053630:Depsides / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006801:Humans; D008136:Longevity / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009043:Motor Activity / Q000187:drug effects / Q000235:genetics; D009046:Motor Neurons / Q000187:drug effects / Q000473:pathology; D009069:Movement Disorders / Q000188:drug therapy* / Q000209:etiology*; D009154:Mutation / Q000235:genetics; D008517:Phytotherapy; D011597:Psychomotor Performance / Q000187:drug effects / Q000502:physiology; D027542:Rosmarinus / Q000737:chemistry; D013116:Spinal Cord / Q000473:pathology; D013482:Superoxide Dismutase / Q000235:genetics; D000097123:Rosmarinic Acid
D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D013482:Superoxide Dismutase; D000097123:Rosmarinic Acid; C109415:SOD1 G93A protein