[PCR rDNA 16S used for the etiological diagnosis of blood culture negative endocarditis].
We report the case of a 55 year-old man presenting with a double aortic and mitral endocarditis for which resected valve culture was repeatedly negative. Specific PCR made on valves because of highly positive blood tests for Bartonella henselae remained negative. A molecular approach was made with 16S rDNA PCR, followed by sequencing. Bartonella quintana was identified as the etiology of endocarditis. B. quintana, "fastidious" bacteria, even if hard to identify in a laboratory, is often reported as a blood culture negative endocarditis (BCNE) agent. Molecular biology methods have strongly improved the diagnosis of BCNE. We propose a review of the literature focusing on the interest of broad-spectrum PCR on valve for the etiological diagnosis of BCNE.
Service de bactériologie-virologie, hôpital Bretonneau, CHRU de Tours, Tours, France.
G
G
Baty
P
P
Lanotte
L
L
Hocqueloux
T
T
Prazuck
L
L
Bret
M
M
Romano
L
L
Mereghetti
D000382:Agricultural Workers' Diseases / Q000097:blood / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology; D001021:Aortic Valve / Q000382:microbiology / Q000601:surgery; D018413:Bartonella quintana / Q000235:genetics / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D004697:Endocarditis, Bacterial / Q000097:blood / Q000209:etiology* / Q000382:microbiology / Q000601:surgery; D005188:False Negative Reactions; D051639:Gardening; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D008943:Mitral Valve / Q000382:microbiology / Q000601:surgery; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D021521:Ribotyping; D012689:Sequence Homology, Nucleic Acid; D014205:Trench Fever / Q000150:complications / Q000175:diagnosis*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S