In vivo efficacy of Trachyspermum ammi anticalcifying protein in urolithiatic rat model.
BACKGROUND
Many medicinal plants have been employed during ages to treat urinary stones though the rationale behind their use is not well established. Recently, we have successfully purified an anticalcifying protein from the seeds of Trachyspermum ammi (L.) Sprague ex Turril (Umbelliferae) using oxalate depletion assay and deciphered its inhibitory activity against calcium oxalate crystal growth.


OBJECTIVE
In this report, the antilithiatic activity of Trachyspermum ammi anticalcifying protein (TAP) was studied in urolithiatic rat model.


METHODS
Urolithiasis was induced by exposure of 0.4% ethylene glycol (EG) and 1.0% ammonium chloride (NH(4)Cl) for 9 days. The efficacy of TAP was studied in another group given same dose of EG and NH(4)Cl in addition to 2mg/kg body weight of TAP. Further, we evaluated ability of TAP to inhibit the attachment of calcium oxalate (CaO(x)) crystal in kidney tissue and studied the consequences of CaO(x) adhesion on renal functioning and tissue integrity.


RESULTS
The antilithiatic potential of TAP was confirmed by its ability to maintain renal functioning, reduce renal injury and decrease crystal excretion in urine and retention in renal tissues.


CONCLUSIONS
Thus, the present investigation suggests the potential of TAP in preventing calcium oxalate deposition and forms the basis for the development of antilithiatic drug interventions against urolithiasis.
Kaur
Tanzeer
T
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat 173215, Solan, India.
Bijarnia
Rakesh K
RK
Singla
Surinder K
SK
Tandon
Chanderdeep
C
D000818:Animals; D019661:Apiaceae / Q000737:chemistry* / Q000196:embryology; D002114:Calcinosis / Q000188:drug therapy*; D004195:Disease Models, Animal; D008297:Male; D010940:Plant Proteins / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D052878:Urolithiasis / Q000188:drug therapy*
D010940:Plant Proteins