Revision of analytical conditions for determining ligand molecules specific to soft metal ions using dequenching of copper(I)-bathocuproine disulfonate as a detection system.
Reoptimization of analytical conditions was performed for a high-performance liquid chromatographic (HPLC) detection system for Cu(I) chelators based on the dequenching of Cu(I)-bathocuproine disulfonate complexes that occurs in the presence of Cu(I) chelators. The revision corrects for emission and excitation wavelengths that were in fact second-order light of the actual optimal wavelengths and for the composition of the postcolumn solution. These revisions resulted in an order of magnitude decrease in detection limits of phytochelatins, a class of cysteine-rich, heavy metal-binding peptides. The revised technique is capable of phytochelatin quantitation at femtomole quantities.
Shinya
S
Ogawa
Rina
R
Ichiki
Mitsuru
M
Abo
Etsuro
E
Yoshimura
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D008024:Ligands; D008670:Metals / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D054811:Phytochelatins / Q000737:chemistry
D008024:Ligands; D008670:Metals; D010618:Phenanthrolines; C028559:bathocuproine sulfonate; D003300:Copper; D054811:Phytochelatins