[Composition diversity of the multifunctional bacterium community NSC-7].
The NSC-7 microbial community could decompose cellulose and lindan with high efficiency. In order to determine the bacterial composition of the community, 11 isolate strains were detected by plate isolation, while a community reset by the 11 isolate strains lost the capacity of degrading cellulose. The capacity of degrading of the filter paper in double deck plate and monolayer plate were determined, only the filter paper in double deck plate were degraded, that means the main or key microbe are anaerobic. The denaturing gradient gel electrophoresis (DGGE) and construction of 16S rDNA clone library were used to identify the composition diversity of NSC-7 community. 195 clones and 25 strains were detected in clone library, and about 60% closest relative among them was known the detailed information which were belonged to Clostridium, Petrobacter, Bacteria, Paenibacillus, Proteobacterium. Furthermore, there were 40% closest relative belonged to uncultured bacterium clone.
College of Life Science, Northeast Forestry University, Harbin 150040,China. liuchangli08@yahoo.com.cn
Chang-Li
CL
Liu
Xiao-Fen
XF
Wang
Jun-Ling
JL
Niu
Yu-Cai
YC
Lü
Peng
P
Guo
Hai-Long
HL
Shen
Zong-Jun
ZJ
Cui
D001419:Bacteria / Q000145:classification*; D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D044822:Biodiversity; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005285:Fermentation; D001556:Hexachlorocyclohexane / Q000378:metabolism; D056507:Paenibacillus / Q000302:isolation & purification; D020560:Proteobacteria / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S; D001556:Hexachlorocyclohexane; D002482:Cellulose