[Evaluation of the ELISA and the enhanced chemiluminescence immunoassay in use to determine the serum markers for liver fibrosis].
OBJECTIVE
To evaluate the detection method of ELISA and Enhanced Chemiluminescence Immunoassay (ECLIA) in use to determine serum hyaluronate acid (HA), laminin (LN), type IV collagen (IV-C) and type III procollagen (PC III).


METHODS
253 patients with chronic hepatitis B were determined the four liver fibrosis serum markers with both the ECLIA and ELISA, and then compared with pathology results separately.


RESULTS
Both the detection results of ELISA and ECLIA can reflect that the patient's liver fibrosis from hepatitis to liver cirrhosis aggravated gradually. Compared with ELISA, the results of ECLIA and pathology have a better correlation.


CONCLUSIONS
The detection of four liver fibrosis serum markers by ECLIA could indicate the better the response of the state of live fibrosis.
Guo
Jing-Xia
JX
Center of Clinical Laboratory, The No. 302 Hospital of The PIA, Beijing 100039, China.
Ma
Hong-Bin
HB
Li
Yong-Li
YL
Xu
Jun
J
Yang
Li-Hua
LH
Shi
Jia-Bin
JB
Li
Bo
B
Liu
Ai-Xia
AX
Li
Xiao-Xi
XX
Li
Bo-An
BA
Mao
Yuan-Li
YL
D015415:Biomarkers / Q000097:blood*; D024061:Collagen Type III / Q000097:blood; D024141:Collagen Type IV / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007797:Laminin / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D008163:Luminescent Measurements / Q000379:methods*
D015415:Biomarkers; D024061:Collagen Type III; D024141:Collagen Type IV; D007797:Laminin