A robust and fast bacteria counting method using CdSe/ZnS core/shell quantum dots as labels.
A rapid, sensitive fluorescence measurement method for detecting the bacterial count using CdSe/ZnS as a fluorescence marker was described. High-quality CdSe/ZnS nanocrystals were synthesized and successfully conjugated with bacteria. The fluorescence intensity was proportional to bacterial count in the range of 10(2)-10(8)CFU/mL and the low detection limit was 10(2)CFU/mL.
Department of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. fuxin1129@hotmail.com
Xin
X
Fu
Kelong
K
Huang
Suqin
S
Liu
D019187:Cadmium Compounds / Q000737:chemistry*; D015169:Colony Count, Microbial / Q000379:methods*; D004926:Escherichia coli / Q000166:cytology*; D016014:Linear Models; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide