Improving the detection of hydrogen peroxide of screen-printed carbon paste electrodes by modifying with nonionic surfactants.
Nonionic surfactants, such as Triton X-100 and Tween-20, were shown in this study to improve the electrocatalytic activity of screen-printed carbon paste electrodes (SPCE). The electrochemical response of SPCE to hydrogen peroxide increased 8-10-fold with the modification of nonionic surfactants. In addition, the glucose biosensors fabricated from nonionic surfactant-modified SPCE exhibited 6.4-8.6-fold higher response to glucose than that fabricated from unmodified SPCE. A concentration effect is proposed for nonionic surfactant to bring neutral reactants to the surface of electrode. Moreover, nonionic surfactant-modified SPCE exhibits a capability of repetitive usage and good reproducibility (R.S.D.<5%) in the measurement of H(2)O(2). Interestingly, the nonionic surfactant-modified SPCE exhibited an opposite effect to ascorbic acid, a common electroactive agent, which causes interference during clinical diagnosis. The differential responses of nonionic surfactant-modified SPCE to H(2)O(2) and ascorbic acid suggest its potential in the development of biosensors for clinical diagnosis.
Yuan
Chiun-Jye
CJ
Department of Biological Science and Technology, National Chiao Tung University, 75 Bo Ai Street, Hsinchu 300, Taiwan, ROC. cjyuan@mail.nctu.edu.tw
Wang
Yen-Chi
YC
Reiko
Ohara
O
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D013501:Surface-Active Agents / Q000737:chemistry*
D004800:Enzymes, Immobilized; D013501:Surface-Active Agents; D002244:Carbon; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose