Urine cytokines suggest an inflammatory response in the overactive bladder: a pilot study.
OBJECTIVE
To study the hypothesis of detecting bladder inflammation associated with overactive bladder (OAB) through altered urine levels of cytokines, chemokines, and growth factors.


METHODS
Midstream urine specimens were collected from a prospective study done on eight asymptomatic control subjects and 17 idiopathic OAB patients. The urine was analyzed by a multiplex panel screen for 12 chemokines, cytokines, growth factors, and soluble receptors using Luminex™ xMAP(®) technology. Protein concentration values were normalized to the levels of creatinine.


RESULTS
This analysis revealed a significant elevation of seven key proteins in the urine of OAB patients relative to controls (*P < 0.05). A greater than tenfold elevation was measured in OAB, relative to controls, in the levels of monocyte chemotactic protein-1 (MCP-1), soluble fraction of the CD40 ligand (sCD40L) in urine was obtained from OAB patients relative to controls. At least five fold elevations were detected in the levels of macrophage inflammatory protein (MIP-1β), IL-12p70/p40, IL-5, epidermal growth factor (EGF), and growth-related oncogene GRO-α compared to controls. Significant threefold elevation was also noticed in the urine levels of sIL-2Rα, and IL-10 in the OAB group. The levels of the remaining proteins tested were not statistically significantly different from control values.


CONCLUSIONS
The presence of elevated levels in urine of inflammatory biomarkers involved in inflammation and tissue repair suggests a role for inflammation in OAB, and may help in diagnosis and treatment of this disease.
Department of Urology, William Beaumont Hospital, Royal Oak, MI 48073, USA.
Pradeep
P
Tyagi
Derek
D
Barclay
Ruben
R
Zamora
Naoki
N
Yoshimura
Kenneth
K
Peters
Yoram
Y
Vodovotz
Michael
M
Chancellor
D000328:Adult; D023201:CD40 Ligand / Q000652:urine; D018932:Chemokine CCL2 / Q000652:urine; D054360:Chemokine CXCL1 / Q000652:urine; D016207:Cytokines / Q000652:urine*; D004815:Epidermal Growth Factor / Q000652:urine; D006801:Humans; D018664:Interleukin-12 / Q000652:urine; D015848:Interleukin-5 / Q000652:urine; D008297:Male; D008875:Middle Aged; D053201:Urinary Bladder, Overactive / Q000473:pathology / Q000652:urine*
D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D016207:Cytokines; D015848:Interleukin-5; D023201:CD40 Ligand; D018664:Interleukin-12; D004815:Epidermal Growth Factor