Pentraxin 3 in amniotic fluid: a novel association with intra-amniotic infection and inflammation.
Pentraxin 3 (PTX3) is a soluble pattern recognition receptor (PRR) that has an important role in immunoregulation and vascular integrity. The aim of this study was to determine if PTX3 is present in amniotic fluid (AF) and whether its concentration changes with gestational age (GA), in the presence of preterm or term labor, and in cases of intra-amniotic infection/inflammation (IAI) associated with spontaneous preterm labor (PTL) or preterm prelabor rupture of membranes (PROM).



This cross-sectional study included the following groups: 1) mid-trimester (n=45); 2) uncomplicated pregnancies at term with (n=48) and without (n=40) spontaneous labor; 3) women with PTL and intact membranes who: a) delivered at term (n=44); b) delivered preterm without IAI (n=40); or c) delivered preterm with IAI (n=62); 4) women with preterm PROM with (n=63) and without (n=36) IAI. PTX3 concentration in AF was determined by ELISA. Non-parametric statistics were used for analyses.



1) Among women with PTL and intact membranes, the median AF PTX3 concentration was significantly higher in women with IAI than in those without IAI (7.95 ng/mL vs. 0.38 ng/mL; P<0.001) and than in those who delivered at term (0.55 ng/mL; P<0.001); 2) women with preterm PROM and IAI had a higher median AF PTX3 concentration than those without IAI (9.12 ng/mL vs. 0.76 ng/mL; P<0.001); 3) the median AF PTX3 concentration did not change with GA (mid-trimester: 0.79 ng/mL vs. term not in labor: 0.58 ng/mL; P=0.09); and 4) labor at term was not associated with a significant change of AF PTX 3 concentration (in labor: 0.54 ng/mL vs. not in labor: 0.58 ng/mL, P=0.9).



PTX3 is a physiologic constituent of the AF, and its median concentration is elevated in the presence of IAI, suggesting that PTX3 may play a role in the innate immune response against IAI.
Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, Maryland, USA.
Laura
L
Cruciani
Roberto
R
Romero
Edi
E
Vaisbuch
Juan Pedro
JP
Kusanovic
Tinnakorn
T
Chaiworapongsa
Shali
S
Mazaki-Tovi
Pooja
P
Mittal
Giovanna
G
Ogge
Francesca
F
Gotsch
Offer
O
Erez
Sun Kwon
SK
Kim
Zhong
Z
Dong
Percy
P
Pacora
Ronald F
RF
Lamont
Lami
L
Yeo
Sonia S
SS
Hassan
Gian Carlo
GC
Di Renzo
D000328:Adult; D000650:Amnion / Q000378:metabolism / Q000382:microbiology; D000653:Amniotic Fluid / Q000378:metabolism* / Q000382:microbiology; D001724:Birth Weight; D002097:C-Reactive Protein / Q000032:analysis / Q000378:metabolism*; D002821:Chorioamnionitis / Q000378:metabolism* / Q000382:microbiology; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007752:Obstetric Labor, Premature / Q000378:metabolism* / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000378:metabolism*; D000683:Serum Amyloid P-Component / Q000032:analysis / Q000378:metabolism*; D018709:Statistics, Nonparametric; D055815:Young Adult; D000099153:Pentraxins
D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins