Complex analysis of cyclin D1 expression in mantle cell lymphoma: two cyclin D1-negative cases detected.
OBJECTIVE
The cytogenetic and diagnostic hallmark of mantle cell lymphoma (MCL) is translocation t(11;14)(q13;q32), resulting in overexpression of cyclin D1. Cyclin D1 expression was analysed in 32 cases of MCL.


METHODS
The t(11;14) translocation was detected by fluorescence in situ hybridisation, level of cyclin D1 mRNA by competitive RT-PCR, and level of cyclin D1 and D2 proteins by immunohistochemistry and/or immunoblotting.


RESULTS
In 30 cases, the presence of translocation t(11;14), a high level of cyclin D1 mRNA, and a high level of the cyclin D1 protein were confirmed. Two cyclin D1-negative cases overexpressing cyclin D2 were detected by immunoblotting.


CONCLUSIONS
There are rare cyclin D1-negative cases of MCL overexpressing cyclin D2. Anti-cyclin D1 antibodies with low specificity can bind both cyclin D1 and cyclin D2, thus providing false cyclin D1-positive signals in immunohistochemical analysis.
Department of Pathology, University Hospital Brno Jihlavska 20, 625 00 Brno, Czech Republic.
L
L
Stefancikova
M
M
Moulis
P
P
Fabian
I
I
Falkova
I
I
Vasova
L
L
Kren
J
J
Macak
J
J
Smardova
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D019938:Cyclin D1 / Q000378:metabolism*; D056742:Cyclin D2 / Q000378:metabolism; D005260:Female; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D014178:Translocation, Genetic
D014408:Biomarkers, Tumor; D056742:Cyclin D2; D009363:Neoplasm Proteins; D019938:Cyclin D1