Modulation of immunoresponse in BALB/c mice by oral administration of Fag e 1-glucomannan conjugate.
Maillard-type glycosylation was applied to preparation of hypoallergenic agents from a major buckwheat allergen, Fag e 1. Conjugation with arabinogalactan (AG), xyloglucan (XG), or yeast glucomannan (YGM) successfully decreased in vitro allergenicity of Fag e 1. Determination of IgE titer in the tested allergic mice revealed that YGM was the most effective for in vivo allergenicity of Fag e 1 among these water-soluble polysaccharides. Real-time PCR analysis using a set of primer for IL-4 (a typical Th2 cytokine) or IFN-gamma (a typical Th1 cytokine) showed that expressed mRNA for IL-4 in splenocytes drastically decreased with increasing with Fag e 1-YGM conjugate feeding. In addition, based on a flow-cytometric analysis of T cell subsets in the splenocytes, it was confirmed that the feeding led to an improvement of Th1/Th2 balance in the allergic mice where population of Th1 increased from 2.91% to 4.02%, while that of Th2 decreased from 3.75% to 2.72%. Furthermore, it was revealed that differentiation ratio of regulatory T cell (Treg) in the splenocytes increased from 14.5% to 18.7% by the oral administration. These results indicated that Fag e 1-YGM conjugate can be available for an immunomodulating agent for buckwheat allergy.
Department of Bioscience and Biotechnology, Shinshu University, 8304 Minamiminowamura, Ina, Nagano, Japan 399-4598.
Yasuhiro
Y
Suzuki
Masahiro
M
Kassai
Tahiro
T
Hirose
Shigeru
S
Katayama
Kosuke
K
Nakamura
Hiroshi
H
Akiyama
Reiko
R
Teshima
Soichiro
S
Nakamura
D000284:Administration, Oral; D000485:Allergens / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D052179:Antigens, Plant; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D019612:Fagopyrum / Q000737:chemistry / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D008351:Mannans / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism
D000485:Allergens; D052179:Antigens, Plant; D016207:Cytokines; C492862:Fag e 1 allergen, Fagopyrum esculentum; D008351:Mannans; C022901:(1-6)-alpha-glucomannan