CD36 gene deletion decreases oleoylethanolamide levels in small intestine of free-feeding mice.
Oleoylethanolamide (OEA) is an endogenous lipid mediator that decreases food intake and enhances lipid catabolism. Dietary fat stimulates OEA mobilization in the proximal small intestine, through a mechanism that requires the participation of the membrane glycoprotein CD36 (fatty acid translocase, FAT). CD36 is highly expressed in small-intestinal enterocytes and is involved in fatty acid uptake and intracellular signaling. Here, we analyze the impact of genetic CD36 deletion on OEA production in various mouse tissues under free-feeding conditions and at different times of the light/dark cycle. CD36 ablation decreases OEA levels in jejunum and plasma during the dark phase, when mice consume most of their daily food. CD36 deletion is also associated with reduced OEA levels in kidney, but not in other tissues including duodenum, stomach, adrenals, white and brown fat, heart, liver, pancreas, skeletal muscle and brain. The results underscore the important role of CD36 in jejunal OEA production linked to feeding.
Department of Pharmacology, University of California, Irvine, CA 92697-4625, USA.
Ana
A
Guijarro
Jin
J
Fu
Giuseppe
G
Astarita
Daniele
D
Piomelli
D000818:Animals; D001921:Brain / Q000378:metabolism; D018955:CD36 Antigens / Q000172:deficiency* / Q000235:genetics; D002940:Circadian Rhythm; D015536:Down-Regulation; D063388:Endocannabinoids; D005227:Fatty Acids / Q000378:metabolism; D005247:Feeding Behavior; D017353:Gene Deletion; D007583:Jejunum / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009829:Oleic Acids / Q000097:blood / Q000378:metabolism*
D018955:CD36 Antigens; D063388:Endocannabinoids; D005227:Fatty Acids; D009829:Oleic Acids; C488250:oleoylethanolamide