Lipid peroxidation in cats experimentally infected with Trypanosoma evansi.
The objective of this study was to evaluate the lipid peroxidation and the susceptibility of erythrocytes to in vitro peroxidation as indicators of oxidative damage in erythrocytes and their roles in the pathogenesis of anemia during experimental Trypanosoma evansi infection in cats. Animals were divided into two groups: control and infected with T. evansi. Seven cats were infected with 10(8) trypomastigotes each, and parasitemia was estimated daily for 49 days by microscopic examination of smears. Hematological and biochemical parameters were evaluated for monitoring of the disease. Plasma lipid peroxidation (Thiobarbituric Acid Reactive Substances (TBARS)) and the susceptibility of erythrocytes to in vitro peroxidation were evaluated. Blood samples for analysis were collected at days 21 and 49 post-inoculation. TBARS level, indicated by MDA concentration, was higher in the infected group than in the control group in both analyzed periods, as well as the in vitro erythrocyte peroxidation (P < 0.001). The infected cats had variable degrees of regenerative anemia, which could be explained by the damage in erythrocyte membrane caused by lipid peroxidation.
da Silva
Aleksandro Schafer
AS
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, Brazil. aleksandro_ss@yahoo.com.br
Wolkmer
Patrícia
P
Machado Costa
Márcio
M
Paim
Francine
F
Belmonte Oliveira
Camila
C
Adriel Zanette
Régis
R
Morais Santurio
Janio
J
Dos Anjos Lopes
Sonia Terezinha
ST
Gonzalez Monteiro
Silvia
S
D000818:Animals; D002371:Cat Diseases / Q000469:parasitology / Q000503:physiopathology*; D002415:Cats; D004195:Disease Models, Animal; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D007958:Leukocyte Count; D015227:Lipid Peroxidation; D016877:Oxidants / Q000633:toxicity; D010949:Plasma / Q000737:chemistry; D014345:Trypanosoma / Q000472:pathogenicity*; D014352:Trypanosomiasis / Q000503:physiopathology*
D016877:Oxidants