[High-performance capillary electrophoresis for determining caffeic acid content in compound Yinhuangjiedu decoction].
OBJECTIVE
To establish a method for determining the content of caffeic acid in compound Yinhuangjiedu decoction using high-performance capillary electrophoresis (HPCE).


METHODS
The optimized HPCE for determining caffeic acid content utilized a fused-silica capillary tube (75 microm x 60 cm, effective length of 53 cm) with 20 mmol/L borate as the running buffer (pH=9.18), a constant voltage of 12 kV, a sampling time of 5 s at 25 degrees celsius, and UV detection wavelength at 313 nm.


RESULTS
The linear range of caffeic acid was 20-100 microg/ml.


CONCLUSIONS
HPCE is simple, rapid, and sensitive for quality control of the compound Yinhuangjiedu decoction.
Department of Pharmacy, 458 Hospital of PLA, Guangzhou, China.
Zhuo
Z
Cai
Fang
F
Yang
Fa
F
Yu
Lu-jun
LJ
Lou
D002109:Caffeic Acids / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008516:Medicine, Chinese Traditional
D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; C040048:caffeic acid