Comparative study of c-Fos expression in rat dorsal vagal complex and nucleus ambiguus induced by different durations of restraint water-immersion stress.
Restraint water-immersion stress (RWIS) of rats induces vagally-mediated gastric dysfunction. The present work explored the effects of different durations of RWIS on neuronal activities of the dorsal vagal complex (DVC) and the nucleus ambiguous (NA) in rats. Male Wistar rats were exposed to RWIS for 0, 30, 60, 120, or 180 min. Then, a c-Fos immunoperoxidase technique was utilized to assess neuronal activation. Resumptively, c-Fos expression in DVC and NA peaked at 60 min of stress, subsequently decreased gradually with increasing durations of RWIS. Interestingly, the most intense c-Fos expression was observed in the dorsal motor nucleus of the vagus (DMV) during the stress, followed by NA, nucleus of solitary tract (NTS) and area postrema (AP). The peak of c-Fos expression in caudal DMV appeared at 120 min of the stress, slower than that in rostral and intermediate DMV. The c-Fos expression in intermediate and caudal NTS was significantly more intense than that in rostral NTS. These results indicate that the neuronal hyperactivity of DMV, NA, NTS and AP, the primary center that control gastric functions, especially DMV and NA, may play an important role in the disorders of gastric motility and secretion induced by RWIS.
Key Laboratory of Animal Resistance of Shandong Province, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong, The People's Republic of China.
Yu-Yu
YY
Zhang
Guo-Hong
GH
Cao
Wen-Xing
WX
Zhu
Xi-Yun
XY
Cui
Hong-Bin
HB
Ai
D000055:Accessory Nerve / Q000378:metabolism*; D000818:Animals; D031608:Area Postrema / Q000378:metabolism; D005930:Glossopharyngeal Nerve / Q000378:metabolism*; D007101:Immersion / Q000503:physiopathology*; D008297:Male; D023421:Models, Animal; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical / Q000502:physiology; D017552:Solitary Nucleus / Q000378:metabolism; D013270:Stomach / Q000294:innervation / Q000503:physiopathology; D013312:Stress, Physiological / Q000502:physiology; D014630:Vagus Nerve / Q000378:metabolism*
D016760:Proto-Oncogene Proteins c-fos