Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil.
The taxonomic positions of two gamma- and UV-ray-resistant strains isolated from radiation-polluted soil in north-west China were determined in a polyphasic study. The organisms, designated R12(T) and R13(T), were Gram-stain-positive, non-spore-forming cocci, which contained MK-8 as the major respiratory quinone and C(16 : 1)omega7c and C(16 : 0) as major fatty acids. The cell walls of strains R12(T) and R13(T) contained ornithine. Phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridizations showed that strains R12(T) and R13(T) are members of novel species belonging to the genus Deinococcus, with Deinococcus radiodurans DSM 20539(T) as the closest relative. The isolates R12(T) and R13(T) shared 97 and 97.1 % 16S rRNA gene similarity, respectively, and 29.5 and 33.3 % DNA-DNA relatedness, respectively, with D. radiodurans DSM 20539(T). The DNA G+C contents of isolates R12(T) and R13(T) were 66.7 and 63.8 %, respectively. On the basis of phenotypic tests and other results, two species, Deinococcus wulumuqiensis sp. nov. (type strain R12(T) =CGMCC 1.8884(T) =NBRC 105665(T)) and Deinococcus xibeiensis sp. nov. (type strain R13(T) =CGMCC 1.8885(T) =NBRC 105666(T)), are proposed.
Wang
Wei
W
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.|Nanjing University of Technology, No. 5 Xinmofan Road, Nanjin 210009, PR China.
Mao
Jun
J
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Zhang
Zhidong
Z
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Tang
Qiyong
Q
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Xie
Yuqing
Y
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Zhu
Jing
J
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
Zhang
Lixin
L
Drug Discovery Centre for TB, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Liu
Zhiheng
Z
Drug Discovery Centre for TB, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Shi
Yuhu
Y
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.|Nanjing University of Technology, No. 5 Xinmofan Road, Nanjin 210009, PR China.
Goodfellow
Michael
M
School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012990:Soil Pollutants, Radioactive / Q000032:analysis*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012990:Soil Pollutants, Radioactive