Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine.
Gel-based and membrane-based flow-through immunoassay formats were investigated for rapid ochratoxin A (OTA) detection in red wine. The flow-through set-up consisted of an antibody containing gel or membrane placed at the bottom of a standard solid-phase extraction column (i.e. the flow-through column), combined with a clean-up column. Different clean-up methods were studied for red wine clarification and purification. The optimal method consisted of passing wine, diluted with an aqueous solution containing 1% polyethylene glycol (PEG 6000) and 5% sodium hydrogencarbonate, through strong anion exchange (SAX) silica. An immunoassay for OTA detection in red wine was optimized and a cut-off level at 2 microg L(-1) according to EU legislation was achieved with both formats. A more significant colour difference between blank and spiked samples was observed for the gel-based assay making this superior to the membrane-based assay. The proposed rapid gel-based test was compared with a standard immunoaffinity column-high-performance liquid chromatography-fluorescent detection (IAC-HPLC-FLD) method and a good correlation of the results was obtained for naturally contaminated wine samples.
Rusanova
T Yu
TY
Saratov State University, Chemistry Institute , Astrakhanskaya 83, 410012 Saratov, Russia. tatyanarys@yandex.ru
Beloglazova
N V
NV
Goryacheva
I Yu
IY
Lobeau
M
M
Van Peteghem
C
C
De Saeger
S
S
D000907:Antibodies, Bacterial / Q000276:immunology / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction; D013050:Spectrometry, Fluorescence; D014920:Wine / Q000032:analysis*
D000907:Antibodies, Bacterial; D009793:Ochratoxins; C025589:ochratoxin A