Cortactin is involved in transforming growth factor-beta1-induced epithelial-mesenchymal transition in AML-12 cells.
Cortactin is an F-actin binding protein, regulating cell movement and adhesive junction assembly. However, the function of cortactin in epithelial-mesenchymal transition (EMT) remains elusive. Here we found that during transforming growth factor-beta1 (TGF-beta1)- induced EMT in AML-12 murine hepatocytes, cortactin underwent tyrosine dephosphorylation. Inhibition of the dephosphorylation of cortactin by sodium vanadate blocked TGF-beta1-induced EMT. Knockdown of cortactin by RNAi led to decrease of intercellular junction proteins E-cadherin and Zonula occludens-1 and induced expression of mesenchymal protein fibronectin. Additionally, knockdown of cortactin further promoted TGF-beta1-induced EMT in AML-12 cells, as determined by EMT markers and cell morphological changes. Moreover, migration assay showed that cortactin knockdown promoted the migration of AML-12 cells, and also enhanced TGF-beta1-induced migration. Our study showed the involvement of cortactin in the TGFbeta1- induced EMT.
Zhang
Kehua
K
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Wang
Dongmei
D
Song
Jianguo
J
D002454:Cell Differentiation / Q000502:physiology*; D054338:Cell Transdifferentiation / Q000502:physiology*; D051356:Cortactin / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D051548:Histone Acetyltransferases / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008648:Mesoderm / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000502:physiology; D053773:Transforming Growth Factor beta1 / Q000502:physiology*; D014407:Tumor Cells, Cultured
D051356:Cortactin; D009419:Nerve Tissue Proteins; D053773:Transforming Growth Factor beta1; C538729:ELP3 protein, human; D051548:Histone Acetyltransferases