Functional consequence of plasmid DNA modified site-specifically with 7-deaza-deoxyadenosine at a single, programmable site.
Replacement of a single dA nucleotide positioned at a programmed site in a DNA plasmid with its 7-deaza-analog is described together with its complete resistance to restriction enzymatic cleavage.
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Wee Han
WH
Ang
Stephen J
SJ
Lippard
D004247:DNA / Q000737:chemistry*; D053904:DNA Breaks, Single-Stranded; D011088:DNA Ligases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry*; D014372:Tubercidin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
C053222:2'-deoxytubercidin; C045550:7-deaza-2'-deoxyguanosine; D004247:DNA; D004262:DNA Restriction Enzymes; D011088:DNA Ligases; D003849:Deoxyguanosine; D014372:Tubercidin