Investigation of Van Gogh-like 2 mRNA regulation and localisation in response to nociception in the brain of adult common carp (Cyprinus carpio).
The Van Gogh-like 2 (vangl2) gene is typically associated with planar cell polarity pathways, which is essential for correct orientation of epithelial cells during development. The encoded protein of this gene is a transmembrane protein and is highly conserved through evolution. Van Gogh-like 2 was selected for further study on the basis of consistent regulation after a nociceptive stimulus in adult common carp and rainbow trout in a microarray study. An in situ hybridisation was conducted in the brain of mature common carp (Cyprinus carpio), 1.5 and 3 h after a nociceptive stimulus comprising of an acetic acid injection to the lips of the fish and compared with a saline-injected control. The vangl2 gene was expressed in all brain regions, and particularly intensely in neurons of the telencephalon and in ependymal cells. In the cerebellum, a greater number (P=0.018) of Purkinje cells expressed vangl2 after nociception (n=7) compared with controls (n=5). This regulation opens the possibility that vangl2 is involved in nociceptive processing in the adult fish brain and may be a novel target for central nociception in vertebrates.
University of Liverpool, School of Biological Sciences, The BioScience Building, Liverpool L69 7ZB, UK.
Siobhan C
SC
Reilly
Anja
A
Kipar
David J
DJ
Hughes
John P
JP
Quinn
Andrew R
AR
Cossins
Lynne U
LU
Sneddon
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009928:Organ Specificity; D010146:Pain / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014018:Tissue Distribution
C431577:Ltap protein, mouse; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger