Aberration-free lithography setup for fabrication of holographic diffractive optical elements.
A holographic optical lithography setup with extremely small longitudinal spherical aberration is described. The setup is used for the fabrication of holographic diffractive optical elements intended to collect light emitted from a fluorescence spot located on a biochip surface. A key feature of the optical setup is its ability to simulate a point-source-like fluorescence spot. A detailed description of the setup together with its optical properties are provided. The fluorescence light collection efficiency of the holographic diffractive optical elements produced using this setup is demonstrated.
Systems Toxicology Unit, Institute for Health and Consumer Protection, European Commission-DG Joint Research Centre, I-21027 Ispra (VA), Italy. peter.macko@jrc.ec.europa.eu
Peter
P
Macko
Maurice P
MP
Whelan
D016880:Anisotropy; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D006696:Holography / Q000379:methods*; D007834:Lasers; D007909:Lenses; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods; D055095:Optics and Photonics; D011108:Polymers / Q000737:chemistry; D012029:Refraction, Ocular; D012031:Refractometry / Q000379:methods; D013997:Time Factors
D005456:Fluorescent Dyes; D011108:Polymers