Expression pattern of antibacterial genes in the Musca domestica.
This work studied the transcriptional patterns of three antibacterial genes, attacin, defensin and cecropin, during the development of Musca domestica. Quantitative analysis by real-time PCR was performed on mRNA levels in different development stages and challenged 3rd-instar larva at different time points after challenge of Musca domestica. The results revealed a predominance of the transcripts of all three genes during the 3rd-instar larvae and the adults. In the meanwhile, it revealed the greatest increase in mRNA. The transcript levels increased to 801 times, 1009 times and 2500 times respectively for cecropin, attacin and defensin in 3rd-instar larvae after challenging susceptible bacterium. The results suggested that the transcriptional patterns of Musca domestica antibacterial genes were different during the different growth stages as well as the microbial challenge encountered in 3rd-instar larvae.
Wang
Yan
Y
Institute of Pathogen Biology and Department of Pathogen Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China. sahara81@163.com
Jin
XiaoBao
X
Zhu
JiaYong
J
Zeng
AiHua
A
Chu
FuJiang
F
Yang
XiaoRong
X
Ma
Yan
Y
D000818:Animals; D001483:Base Sequence; D054807:Cecropins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D023082:Defensins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D006793:Houseflies / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D054807:Cecropins; D017931:DNA Primers; D023082:Defensins; D019476:Insect Proteins; D012333:RNA, Messenger; C039711:attacin antibacterial protein, insect