Poly(hydroxyalkanoates)-based polymeric nanoparticles for drug delivery.
Poly (hydroxyalkanoates) (PHAs) have recently attracted a great deal of academic and industrial interest for their biodegradability and biocompatibility making them suitable for environmental and biomedical applications. Poly(3-hydroxybutyrate-) (PHB-) and Poly(DL-lactide-co-glycolide) (PLGA-) based nanoparticles were prepared using the dialysis method as yet unreported for the preparation of nanoparticles based on PHB. Processing conditions were varied in order to evaluate their influence on morphology, drug encapsulation, and size of nanoparticles. The relevant results obtained give a theoretical understanding of the phenomenon occurring during colloidal formation. The adopted procedure allows for a relatively small diameter and homogeneity in size distribution of the PHB nanoparticles to be obtained compared to other methods like the one based on solvent evaporation which leads to particles on microscale. The biocompatibility of PHB and relative nanoparticles was investigated and both exhibited very good cytocompatibility.
Laboratory of Bioactive Polymeric Materials for Biomedical & Environmental Applications, UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Via Risorgimento 35, 56126 Pisa, Italy.
Cesare
C
Errico
Cristina
C
Bartoli
Federica
F
Chiellini
Emo
E
Chiellini
D016475:3T3 Cells; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D003956:Dialysis; D016503:Drug Delivery Systems / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000494:pharmacology; D014212:Tretinoin / Q000737:chemistry / Q000494:pharmacology; D014867:Water / Q000737:chemistry
D054813:Polyhydroxyalkanoates; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014212:Tretinoin