Chemoselective ligand patterning of electroactive surfaces using microfluidics.
To generate model substrates for cell adhesion, we have developed two different biocompatible strategies based on self-assembled monolayers (SAMs) of alkanethiolates on gold terminated with latent ketones and aldehydes. Under spatial control, the hydroquinone and alcohol-terminated SAMs can be oxidized to allow for oxyamine ligand patterning on the surface with microfluidic cassettes. These immobilization strategies were characterized by electrochemistry, fluorescence, and utilizing a cell adhesive peptide, cell patterns were generated.
Department of Chemistry and the Carolina Center for Genome Science, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
Nathan P
NP
Westcott
Muhammad N
MN
Yousaf
D000268:Adhesiveness; D000438:Alcohols / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion; D004563:Electrochemistry / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology; D006873:Hydroquinones / Q000737:chemistry; D008024:Ligands; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D010084:Oxidation-Reduction; D041701:Swiss 3T3 Cells
D000438:Alcohols; D006873:Hydroquinones; D008024:Ligands; C031927:hydroquinone