Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.).
To understand the development of rice leaf blades, we identified a new rolled-leaf mutant, w32, from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene, bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene, tentatively named rl11(t), is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers, the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway.
Chinese National Center for Rice Improvement/National key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.
YongFeng
Y
Shi
Jie
J
Chen
WenQiang
W
Liu
QiNa
Q
Huang
Bo
B
Shen
Hei
H
Leung
JianLi
J
Wu
D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005020:Ethyl Methanesulfonate; D017343:Genes, Plant; D006824:Hybridization, Genetic; D016296:Mutagenesis; D009153:Mutagens; D009154:Mutation; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics*; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology
D017931:DNA Primers; D018744:DNA, Plant; D009153:Mutagens; D005020:Ethyl Methanesulfonate