Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells.
BACKGROUND
The aim of this study was to evaluate and compare the cytotoxic effects of BioAggregate (Innovative BioCaramix Inc, Vancouver, BC, Canada), a novel bioceramic nanoparticulate cement, on human mesenchymal cells. White Pro-Root MTA (Dentsply, Tulsa Dental, Tulsa, OK) was used as a reference for comparison.


METHODS
Fifty-six human maxillary incisor teeth were submitted to a step-back flaring technique and prepared for cytotoxicity assay in an in situ root-end filling experimental model. After retro filling, each root containing MTA, BioAggregate, or empty root canals (control) was exposed to culture media for 24, 48, or 72 hours, providing several extraction media. Mesenchymal cells were incubated with each extract medium for 24 hours, and toxicity was evaluated by three different parameters of cell survival and integrity on the same sample: XTT, neutral red, and crystal violet dye elution.


RESULTS
No statistically significant differences between MTA and BioAggregate were found in all the experimental periods (p > 0.05).


CONCLUSIONS
DiaRoot BioAggregate displayed in vitro compatibility similar to MTA.
De-Deus
Gustavo
G
Department of Endodontics, Veiga de Almeida University, Rio de Janeiro, Brazil.
Canabarro
Antonio
A
Alves
Gutemberg
G
Linhares
Adriana
A
Senne
Maria Isabel
MI
Granjeiro
Jose Mauro
JM
D017607:Aluminum Compounds / Q000494:pharmacology; D001672:Biocompatible Materials / Q000633:toxicity*; D017610:Calcium Compounds / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D003738:Dental Cements / Q000633:toxicity*; D004338:Drug Combinations; D006801:Humans; D008422:Materials Testing; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects*; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000494:pharmacology; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; C022616:thiazolyl blue