The poplar GT8E and GT8F glycosyltransferases are functional orthologs of Arabidopsis PARVUS involved in glucuronoxylan biosynthesis.
The poplar GT8E and GT8F glycosyltransferases have previously been shown to be associated with wood formation, but their roles in the biosynthesis of wood components are not known. Here, we show that PoGT8E and PoGT8F are expressed in vessels and fibers during wood formation and their encoded proteins are predominantly located in the endoplasmic reticulum. We demonstrate that expression of PoGT8E and PoGT8F in the Arabidopsis parvus mutant rescues the defects in the content and structure of glucuronoxylan conferred by the parvus mutation. These findings suggest that PoGT8E and PoGT8F are involved in glucuronoxylan biosynthesis during wood formation in poplar.
Lee
Chanhui
C
Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
Teng
Quincy
Q
Huang
Wenlin
W
Zhong
Ruiqin
R
Ye
Zheng-Hua
ZH
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D029681:Arabidopsis Proteins; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D005816:Genetic Complementation Test; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000201:enzymology* / Q000235:genetics; D014934:Wood / Q000235:genetics / Q000378:metabolism; D014990:Xylans / Q000096:biosynthesis*; D052584:Xylem / Q000166:cytology / Q000378:metabolism
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D010940:Plant Proteins; D014990:Xylans; C038910:glucuronoxylan; C496965:GLZ1 protein, Arabidopsis; D016695:Glycosyltransferases