Short communication: RNA interference directed against Axin1 upregulates human immunodeficiency virus type 1 gene expression by activating the Wnt signaling pathway in HeLa-derived J111 cells.
Axin1, a regulator of Wnt signaling, was previously identified as playing a negative role in the late phase of human immunodeficiency virus type 1 (HIV-1) replication in HeLa-derived J111 cells. In this report, we studied the molecular mechanism of how Axin1 regulates HIV-1 replication. HIV-1 transactivator, Tat-dependent viral reporter gene expression was enhanced in J111 cells transfected with small interfering RNA (siRNA) against Axin1. In addition, viral transcription was upregulated in J111 cells transfected with siRNA against Axin1. In contrast, HIV-1 gene expression was not enhanced by transfecting HeLa cells with siRNA against Axin1. The expression levels of T cell factor-4 (TCF4) and beta-catenin were higher in J111 than HeLa cells. In addition, siRNAs against TCF4 and beta-catenin inhibited the Axin1 siRNA-dependent enhancement of HIV-1 gene expression in J111 cells. These results suggest that Axin1 plays a negative role in HIV-1 transcription through the Wnt signaling pathway in J111 cells under normal cell culture conditions.
Kameoka
Masanori
M
Thailand-Japan Research Collaboration Center on Emerging And Re-emerging Infections, Nonthaburi, Thailand. mkameoka@biken.osaka-u.ac.jp
Kameoka
Yoko
Y
Utachee
Piraporn
P
Kurosu
Takeshi
T
Sawanpanyalert
Pathom
P
Ikuta
Kazuyoshi
K
Auwanit
Wattana
W
D060466:Axin Protein; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000096:biosynthesis; D015967:Gene Expression Regulation, Viral; D015497:HIV-1 / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D034622:RNA Interference; D012097:Repressor Proteins / Q000037:antagonists & inhibitors*; D015398:Signal Transduction; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000096:biosynthesis; D014158:Transcription, Genetic; D015854:Up-Regulation; D051176:beta Catenin / Q000096:biosynthesis; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000096:biosynthesis
C555451:AXIN1 protein, human; D060466:Axin Protein; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D012097:Repressor Proteins; C532096:TCF4 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051176:beta Catenin; D054322:tat Gene Products, Human Immunodeficiency Virus