Enhanced protein immobilization efficiency on a TiO2 surface modified with a hydroxyl functional group.
An antibody immobilization was investigated using a self-assembled monolayer (SAM) over the highly refractive coatings with a SiO2, TiO2, or Si3N4 substrate. The immobilization was characterized by analyzing the hydrophilic properties of hydroxyl (OH) groups on surface coatings with contact angle (CA) measurements to enhance protein immobilization. The hydroxyl (OH) group was formed in greater amounts as the oxygen plasma exposure time was increased, which resulted in a large enhancement in antibody immobilization. It indicated that hydroxyl (OH) group formation is critical for developing a label-free optical transducer with a high sensitivity.
Biosensor Research Team, Electronics and Telecommunications Research Institute, Daejeon 305-700, South Korea. kokwj@etri.re.kr
Wan-Joong
WJ
Kim
Sanghee
S
Kim
Bong Soo
BS
Lee
Ansoon
A
Kim
Chil Seong
CS
Ah
Chul
C
Huh
Gun Yong
GY
Sung
Wan Soo
WS
Yun
D000588:Amines / Q000737:chemistry; D000818:Animals; D055910:Antibodies, Immobilized / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D057927:Hydrophobic and Hydrophilic Interactions; D006878:Hydroxides / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000276:immunology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
D000588:Amines; D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D006878:Hydroxides; C009495:titanium dioxide; C031356:hydroxide ion; D014025:Titanium; D017430:Prostate-Specific Antigen; D010100:Oxygen