Copro-antigen detection versus direct methods for the diagnosis of Giardia lamblia in patients from the National Liver Institute.
In this study a commercially available immunoenzymatic assay to detect G. lamblia specific copro-antigen was evaluated. A total of 90 stool samples were tested. Diagnosis of giardiasis by ELISA for copro-Ag detection was positive in 46 (51.1%) patients whereas by direct stool analysis after formol ether concentration G. lamblia was detected in 38 (42.2%) patients only. ELISA technique for detection of Giardia copro-antigen had a sensitivity of 97.3% and a specificity of 82.6% with PPV of 80.4% and a NPV of 97.7%.
Selim
Sahar
S
Department of Parasitology, National Liver Institute, Egypt.
Nassef
Nashaat
N
Sharaf
Soraya
S
Badra
Gamal
G
Abdel-Atty
Dalia
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000469:parasitology; D005260:Female; D016829:Giardia lamblia / Q000276:immunology*; D005873:Giardiasis / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
D000953:Antigens, Protozoan