Cryopreservation of in vitro shoot apices of Glehnia littoralis - a medical plant.
Cryopreservation was examined as a practical method for preserving the genetic resources of Glehnia littoralis Fr. Schm. a nearly exterminated medical plant. In vitro shoot apices were successfully cryopreserved using vitrification and encapsulation-dehydration. In vitrification, regrowth of apices loaded for 60 min with PVS2 (66.7 +/- 6.7 percent) was preferable to loading with PVS3 (50.0 +/- 5.8 percent). With encapsulation-dehydration, the best regrowth (43.3 +/- 3.3 percent) was achieved when the moisture content in the beads was reduced to 19 percent by drying with silica gel for 6 h. Increased regrowth of shoot tips cryopreserved by encapsulation-dehydration resulted from the addition of 0.1 mM acetylsalicylic acid to the loading solution (86.7 +/- 3.3 percent).
Department of Horticultural Science and Landscape Architecture, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan. otkcanda@res.agr.hokudai.ac.jp
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Otokita
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Kami
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Suzuki
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Suzuki
D019661:Apiaceae / Q000235:genetics / Q000502:physiology*; D001241:Aspirin / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003890:Desiccation; D066298:In Vitro Techniques; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*
D001241:Aspirin