Effect of calcium-modulating cyclophilin ligand on human immunodeficiency virus type 1 particle release and cell surface expression of tetherin.
The human immunodeficiency virus type 1 (HIV-1) accessory protein Vpu enhances virus particle release by counteracting a host factor that retains virions at the surfaces of infected cells. It was recently demonstrated that cellular protein BST-2/CD317/Tetherin restricts HIV-1 release in a Vpu-dependent manner. Calcium-modulating cyclophilin ligand (CAML) was also proposed to be involved in this process. We investigated whether CAML is involved in cell surface expression of Tetherin. Here, we show that CAML overexpression in permissive Cos-7 cells or CAML depletion in restrictive HeLa cells has no effect on HIV-1 release or on Tetherin surface expression, indicating that CAML is not required for Tetherin-mediated restriction of HIV-1 release.
Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montréal, 110, Avenue des Pins Ouest, Montreal, Quebec, Canada H2W 1R7.
Mariana G
MG
Bego
Mathieu
M
Dubé
Johanne
J
Mercier
Eric A
EA
Cohen
D048868:Adaptor Proteins, Signal Transducing / Q000037:antagonists & inhibitors / Q000502:physiology*; D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000502:physiology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D058851:GPI-Linked Proteins; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis / Q000502:physiology*; D014771:Virion / Q000502:physiology*; D000074010:Bone Marrow Stromal Antigen 2
D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2; C089404:CAMLG protein, human