In vivo assessment of the effect of an adhesive for complete dentures on colonisation of Candida species.
OBJECTIVE
Denture adhesives have long been recognised by denture wearers as a useful adjunct to denture retention and stability. The objective of the present study was to evaluate, in vivo, the effect of a denture adhesive on oral quantities of Candida species by determination of absolute counts of colony-forming units (CFU) per ml of saliva of individuals who use this denture adhesive for a period of 14 days.


METHODS
Twenty-four individuals were randomised in two equal groups of 12 (test and control), with the individuals of the test group using the adhesive for 14 days. Samples of saliva were collected from all individuals on days 0 (initial), 7 and 14. Aliquots of saliva were diluted and plated in duplicate on Sabouraud dextrose agar with chloramphenicol and incubated for 37 °C for 48 h, the CFU/ml were counted in the individuals of each group and the data of each group were compared at the different time periods and analysed statistically by the non-parametric Mann-Whitney U-test (α ≤ 5%).


RESULTS
There were no statistically significant differences between the test and control groups during the test periods.


CONCLUSIONS
Within of the limitations of this study, the data suggested that the denture adhesive tested did not significantly alter the oral microbiota during the 14-day trial period.
Oliveira
Mario C S
MC
College of Dentistry, State University of Feira de Santana and Master in Clinical Dentistry, Bahiana School of Medicine and Public Health, Salvador-Ba, Brazil. mcezar11@gmail.com
Oliveira
Viviane M B
VM
Vieira
Alex C
AC
Rambob
Isabel
I
D000269:Adhesives / Q000737:chemistry*; D000368:Aged; D002175:Candida / Q000145:classification / Q000254:growth & development*; D015169:Colony Count, Microbial; D003781:Denture Retention; D003824:Denture, Complete / Q000382:microbiology*; D003920:Diabetes Mellitus / Q000382:microbiology; D005260:Female; D006801:Humans; D006973:Hypertension / Q000382:microbiology; D008297:Male; D008422:Materials Testing; D008875:Middle Aged; D009172:Mycology / Q000379:methods; D012463:Saliva / Q000382:microbiology; D012907:Smoking; D013696:Temperature; D013997:Time Factors
D000269:Adhesives