Fabrication of mesoporous SiO(2)-C-Fe(3)O(4)/gamma-Fe(2)O(3) and SiO(2)-C-Fe magnetic composites.
A synthetic method for the fabrication of silica-based mesoporous magnetic (Fe or iron oxide spinel) nanocomposites with enhanced adsorption and magnetic capabilities is presented. The successful in situ synthesis of magnetic nanoparticles is a consequence of the incorporation of a small amount of carbon into the pores of the silica, this step being essential for the generation of relatively large iron oxide magnetic nanocrystals ( approximately 10+/-3nm) and for the formation of iron nanoparticles. These composites combine good magnetic properties (superparamagnetic behaviour in the case of SiO(2)-C-Fe(3)O(4)/gamma-Fe(2)O(3) samples) with a large and accessible porosity made up of wide mesopores (>9nm). In the present work, we have demonstrated the usefulness of this kind of composite for the adsorption of a globular protein (hemoglobin). The results obtained show that a significant amount of hemoglobin can be immobilized within the pores of these materials (up to 180mgg(-1) for some of the samples). Moreover, we have proved that the composite loaded with hemoglobin can be easily manipulated by means of an external magnetic field.
Sevilla
Marta
M
Instituto Nacional del Carbón (CSIC), Apartado 73, 33080-Oviedo, Spain.
Valdés-Solís
Teresa
T
Tartaj
Pedro
P
Fuertes
Antonio B
AB
D000327:Adsorption; D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000138:chemical synthesis / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D009584:Nitrogen / Q000737:chemistry; D016062:Porosity; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000737:chemistry; D013499:Surface Properties; D014961:X-Ray Diffraction
D004337:Drug Carriers; D005290:Ferric Compounds; D006454:Hemoglobins; C509969:SBA-15; C000499:ferric oxide; D013395:Sucrose; D058428:Silica Gel; D002244:Carbon; D012822:Silicon Dioxide; D009584:Nitrogen; D052203:Ferrosoferric Oxide