PTTH-stimulated ERK phosphorylation in prothoracic glands of the silkworm, Bombyx mori: role of Ca(2+)/calmodulin and receptor tyrosine kinase.
Our previous studies showed that the prothoracicotropic hormone (PTTH) stimulated extracellular signal-regulated kinase (ERK) phosphorylation in prothoracic glands of Bombyx mori both in vitro and in vivo. In the present study, the signaling pathway by which PTTH activates ERK phosphorylation was further investigated using PTTH, second messenger analogs, and various inhibitors. ERK phosphorylation induced by PTTH was partially reduced in Ca(2+)-free medium. The calmodulin antagonist, calmidazolium, partially inhibited both PTTH-stimulated ERK phosphorylation and ecdysteroidogenesis, indicating the involvement of calmodulin. When the prothoracic glands were treated with agents that directly elevate the intracellular Ca(2+) concentration [either A23187, thapsigargin, or the protein kinase C (PKC) activator, phorbol 12-myristate acetate (PMA)], a great increase in ERK phosphorylation was observed. In addition, it was found that PTTH-stimulated ecdysteroidogenesis was greatly attenuated by treatment with PKC inhibitors (either calphostin C or chelerythrine C). However, PTTH-stimulated ERK phosphorylation was not attenuated by the above PKC inhibitors, indicating that PKC is not involved in PTTH-stimulated ERK phosphorylation. A potent and specific inhibitor of insulin receptor tyrosine kinase, HNMPA-(AM)(3), greatly inhibited the ability of PTTH to activate ERK phosphorylation and stimulate ecdysteroidogenesis. However, genistein, another tyrosine kinase inhibitor, did not inhibit PTTH-stimulated ERK phosphorylation, although it did markedly attenuate the ability of A23187 to activate ERK phosphorylation. From these results, it is suggested that PTTH-stimulated ERK phosphorylation is only partially Ca(2+)- and calmodulin-dependent and that HNMPA-(AM)(3)-sensitive receptor tyrosine kinase is involved in activation of ERK phosphorylation by PTTH.
Gu
Shi-Hong
SH
Department of Zoology, National Museum of Natural Science, 1 Kuan-Chien Road, Taichung 404, Taiwan, ROC. gu330@mail.nmns.edu.tw
Lin
Ju-Ling
JL
Lin
Pei-Ling
PL
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D012831:Bombyx / Q000201:enzymology*; D000001:Calcimycin; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D026461:Ecdysteroids / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000276:immunology / Q000378:metabolism*; D019833:Genistein; D007093:Imidazoles; D007301:Insect Hormones / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D009281:Naphthalenes; D063065:Organophosphonates; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000906:Antibodies; D002147:Calmodulin; D026461:Ecdysteroids; D007093:Imidazoles; D007301:Insect Hormones; D009281:Naphthalenes; D063065:Organophosphonates; C519736:hydroxy-2-naphthalenyl-methyl phosphonic acid trisacetoxymethylester; D000001:Calcimycin; C031938:calmidazolium; C009089:prothoracicotropic hormone; D019833:Genistein; D020794:Receptor Protein-Tyrosine Kinases; D011493:Protein Kinase C; D048049:Extracellular Signal-Regulated MAP Kinases; D002118:Calcium