[The analysis of "alpha" dominant mutation of hepatitis B virus in community-based Zhengding area, Hebei province].
OBJECTIVE
To determine the "alpha"dominant mutation of hepatitis B virus (HBV) in community-based Zhengding. Analysis the role of the newborn hepatitis B vaccination on the mutation.


METHODS
Based on the national surveillance of hepatitis B, 11,478 people's sera were collected and tested by SPRIA with kits. Collect people's sera with positive HBsAg and amplify the S gene. Sequencing and clastwaling them with the standard sequences.


RESULTS
Overall, HBV DNA was successfully amplified and sequenced in 434 of 443 samples. 6.7% samples mutated in HBV "alpha" dominant region. The difference between the mutation ratio of the two loops of HBV "alpha" dominant between the people born before and after the year 1986 has no significance.


CONCLUSIONS
There were HBV "alpha" dominant mutant virus in the local area with a low infection rate in the population born after the year 1986. It could not explain the newborn hepatitis B vaccination can induce the prevalence of the "alpha" dominant mutate HBV.
Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Hong-Xia
HX
Yan
Jing-Chen
JC
Ma
Tao
T
Bian
Chang-Hong
CH
Huang
Shuang
S
Zhang
Zhi-Yong
ZY
Zhang
Zhi-Yong
ZY
Hao
Ji-Chao
JC
Chen
Yong
Y
Zhang
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D006509:Hepatitis B / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D055815:Young Adult
D006514:Hepatitis B Surface Antigens