High-throughput microarray profiling of cell wall polymers during hydrothermal pre-treatment of wheat straw.
Lignocellulosic plant material is potentially a sustainable source of fermentable sugars for bioethanol production. However, a barrier to this is the high resistance or recalcitrance of plant cell walls to be hydrolyzed. Therefore, a detailed knowledge of the structural features of plant cell walls that contribute to recalcitrance is important for improving the efficiency of bioethanol production. In this work we have used a technique known as Comprehensive Microarray Polymer Profiling (CoMPP) to analyze wheat straw before and after being subjected to hydrothermal pre-treatments at four different temperatures. The CoMPP technique combines the specificity of monoclonal antibodies with the high-throughput capacity of microarrays. Changes in the relative abundance of cell wall polysaccharides could be tracked during processing, and a reduction in xylan, arabinoxylans, xyloglucan, and mixed-linked glucan epitopes was detected at the two highest temperatures of pre-treatment used. This work demonstrates the potential of CoMPP as a complementally technique to conventional methods for analyzing biomass composition.
Department of Biology, University of Copenhagen, Denmark.
Ana
A
Alonso-Simón
Jan Bach
JB
Kristensen
Jens
J
Obro
Claus
C
Felby
William G T
WG
Willats
Henning
H
Jørgensen
D000911:Antibodies, Monoclonal / Q000276:immunology; D001709:Biotechnology / Q000379:methods*; D002473:Cell Wall / Q000737:chemistry*; D006358:Hot Temperature; D018547:Plant Stems / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods; D014908:Triticum / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D011134:Polysaccharides