Screening of a large global Aspergillus fumigatus species complex collection by using a species-specific microsphere-based Luminex assay.
A microsphere-based Luminex assay was developed and validated for rapid identification of Aspergillus fumigatus from the other species within the A. fumigatus species complex (section Fumigati). This molecular tool was then employed to screen 499 clinical A. fumigatus species complex isolates collected from multiple medical centers throughout the world with results demonstrating the exclusive presence of A. fumigatus.
Etienne
Kizee A
KA
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Gade
Lalitha
L
Lockhart
Shawn R
SR
Diekema
Daniel J
DJ
Messer
Shawn A
SA
Pfaller
Michael A
MA
Balajee
S Arunmozhi
SA
D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D006801:Humans; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D004271:DNA, Fungal