Preservation of platelets by adding epigallocatechin-3-o-gallate to platelet concentrates.
The effect of epigallocatechin-3-O-gallate (EGCG), a major component of green tea, on platelet preservation was evaluated. Single donor platelets (N = 10) were collected and preserved by the standard method. EGCG was added to the platelet concentrates before preservation and then the functional and biochemical parameters were monitored throughout the storage period. After 6 days of preservation, the aggregability of the platelets was significantly maintained by addition of 50 and 100 microg/ml of EGCG. Platelet prothrombinase activity was also significantly retained by the addition of EGCG. The accumulation of P-selectin and RANTES in the plasma preserved with EGCG was less than those preserved without EGCG, which indicated that EGCG might inhibit platelet activation. Furthermore, EGCG reduced the increase of LDH in plasma during preservation and inhibited the activation of caspase-3 and cleavage of gelsolin, thereby showing that EGCG could inhibit the apoptosis of platelets. These results suggest that EGCG may play an effective role in preserving platelets by inhibiting the activation and apoptosis of platelets.
Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
Kazuaki
K
Matsumura
Hiroshi
H
Takayama
Jung Yoon
JY
Bae
Mitsuru
M
Kurihara
Sadami
S
Tsutsumi
Suong-Hyu
SH
Hyon
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D001793:Blood Preservation / Q000379:methods*; D053148:Caspase 3 / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D018946:Chemokine CCL5 / Q000378:metabolism; D018260:Gelsolin / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D013925:Thromboplastin / Q000378:metabolism
D000975:Antioxidants; D018946:Chemokine CCL5; D018260:Gelsolin; D019007:P-Selectin; D002392:Catechin; D013925:Thromboplastin; C045651:epigallocatechin gallate; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3