Correlation between an in vitro and an in vivo measure of dioxin sensitivity in birds.
We describe a statistically significant correlation between two well-characterized responses to dioxin-like compounds in birds; induction of 7-ethoxyresorufin-O-deethylase (EROD) activity in cultured hepatocytes, and embryo mortality. Data were obtained from a review of the literature. EROD EC50 values for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 6 polychlorinated biphenyls (PCBs) were strongly correlated with LD50 values in chicken embryos (r(2) = 0.93, P < 0.005). Similarly, EROD EC50 values for TCDD and a potent dioxin-like compound, PCB 126, were correlated with embryonic LD50 values in different species of birds (chicken, ring-necked pheasant, turkey, double-crested cormorant, and common tern) (r(2) = 0.92, P < 0.005). Our findings contribute to a developing understanding of the molecular basis for differential dioxin sensitivity in birds, and validate the EROD bioassay as a useful predictive tool for ecological risk assessment.
Cooperative Institute for Limnology and Ecosystem Research, University of Michigan and NOAA's Great Lakes Environmental Research Laboratory, Ann Arbor, MI, USA. jesshead@umich.edu
Jessica A
JA
Head
Sean W
SW
Kennedy
D000818:Animals; D001681:Biological Assay; D001717:Birds / Q000145:classification / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000378:metabolism*; D004147:Dioxins / Q000378:metabolism / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism / Q000633:toxicity; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism / Q000633:toxicity; D018570:Risk Assessment; D013045:Species Specificity; D015996:Survival Rate; D014421:Turkey; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1