Peptidomics and genomics analysis of novel antimicrobial peptides from the frog, Rana nigrovittata.
Much attention has been paid on amphibian peptides for their wide-ranging pharmacological properties, clinical potential, and gene-encoded origin. More than 300 antimicrobial peptides (AMPs) from amphibians have been studied. Peptidomics and genomics analysis combined with functional test including microorganism killing, histamine-releasing, and mast cell degranulation was used to investigate antimicrobial peptide diversity. Thirty-four novel AMPs from skin secretions of Rana nigrovittata were identified in current work, and they belong to 9 families, including 6 novel families. Other three families are classified into rugosin, gaegurin, and temporin family of amphibian AMP, respectively. These AMPs share highly conserved preproregions including signal peptides and spacer acidic peptides, while greatly diversified on mature peptides structures. In this work, peptidomics combined with genomics analysis was confirmed to be an effective way to identify amphibian AMPs, especially novel families. Some AMPs reported here will provide leading molecules for designing novel antimicrobial agents.
Ma
Yufang
Y
Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
Liu
Cunbao
C
Liu
Xiuhong
X
Wu
Jing
J
Yang
Hailong
H
Wang
Yipeng
Y
Li
Jianxu
J
Yu
Haining
H
Lai
Ren
R
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D023281:Genomics; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000494:pharmacology; D040901:Proteomics; D011817:Rabbits; D011898:Ranidae / Q000235:genetics; D051381:Rats; D020539:Sequence Analysis, Protein; D012867:Skin / Q000737:chemistry
D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D010455:Peptides