Heat-shock factor 1 controls genome-wide acetylation in heat-shocked cells.
A major regulatory function has been evidenced here for HSF1, the key transcription factor of the heat-shock response, in a large-scale remodeling of the cell epigenome. Indeed, upon heat shock, HSF1, in addition to its well-known transactivating activities, mediates a genome-wide and massive histone deacetylation. Investigating the underlying mechanisms, we show that HSF1 specifically associates with and uses HDAC1 and HDAC2 to trigger this heat-shock-dependent histone deacetylation. This work therefore identifies HSF1 as a master regulator of global chromatin acetylation and reveals a cross-talk between HSF1 and histone deacetylases in the general control of genome organization in response to heat shock.
Fritah
Sabrina
S
Institut National de la Santé et de la Recherche Médicale, U823, Institut Albert Bonniot, Université Joseph Fourier, F-38706 Grenoble, France.
Col
Edwige
E
Boyault
Cyril
C
Govin
Jérôme
J
Sadoul
Karin
K
Chiocca
Susanna
S
Christians
Elisabeth
E
Khochbin
Saadi
S
Jolly
Caroline
C
Vourc'h
Claire
C
D000107:Acetylation; D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D016678:Genome; D000076249:Heat Shock Transcription Factors; D018869:Heat-Shock Response / Q000235:genetics*; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism; D056464:Histone Deacetylase 2 / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D002843:Chromatin; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D006657:Histones; C490017:Hsf1 protein, mouse; D034741:RNA, Small Interfering; D014157:Transcription Factors; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2