[Preparation and identification of the monoclonal antibodies against VP1 capsid protein of Enterovius 71].
OBJECTIVE
To prepare monoclonal antibodies (McAbs) against VP1 capsid protein of Enterovirus 71.


METHODS
Two peptides, SP55 and SP70, containing amino acid 163-177 and 208-222 of VP1, were synthesized respectively. Immunized BALB/c mice with the synthetic peptides to establish the hybridoma cell strains secreting specific McAb to VP1. After the specific McAbs were prepared, identified and analyzed the titer by indirect ELISA assay. The positive clones were selected and their neutralization titer were determined by neutralization test.


RESULTS
Two high titered anti-VP1 antibodies secreted by the hybridoma cells showed good neutralization reaction with enterovirus 71 on RD cells, and the neutralization titer were 1:8 and 1:16 respectively.


CONCLUSIONS
Two high titered anti-VP1 antibodies, with good neutralization activity, secreted by the hybridoma cells, which lays the foundation for further study.
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Yao
Y
Yi
Min-Zhuo
MZ
Guo
Xin-Liang
XL
Shen
Tao
T
Yu
Zhi-Yuan
ZY
Jia
Sheng-Li
SL
Bi
Xiu-Ling
XL
Li
Si-Yong
SY
Chen
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D036022:Capsid Proteins / Q000032:analysis* / Q000276:immunology; D004770:Enterovirus / Q000737:chemistry* / Q000276:immunology; D004769:Enterovirus Infections / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins