Protective effects of selenium and vitamin E combination on experimental colitis in blood plasma and colon of rats.
Ulcerative colitis increases oxidative damage accompanied by production of free oxygen radicals. Selenium (Se) and vitamin E are two natural antioxidants. The present study was undertaken to investigate the possible protective role of Se and vitamin E combination in experimental colitis induced by acetic acid (AA) in rats. This study was carried out on three groups, namely the first (control), the second (experimental colitis group, 2 ml 5% acetic acid), and the third groups (2 ml 5% acetic acid, vitamin E (100 mg/kg body weight (bw)) plus Se (0.2 mg/kg bw)). The activities of catalase (CAT), prolidase (PRS), myeloperoxidase (MPO), total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), total thiol (T-SH) were determined in plasma and colon samples. Macroscopic and microscopic damages in colon were increased by AA treatment (p < 0.01 and p < 0.01, respectively), whereas they were decreased by selenium and vitamin E treatment (p < 0.05 and p < 0.01, respectively). The activities of CAT and PRS in the plasma and colon were significantly affected (p < 0.05 and p < 0.01) by treatment of AA, Se, and vitamin E. MPO activity in colon was increased (p < 0.01) by AA treatment and decreased (p < 0.05) by Se and vitamin E administration. The values of TOS and OSI in plasma were increased (p < 0.5) by AA. The TAC and T-SH in colon were decreased (p < 0.05) by AA and increased (p < 0.05) by Se and vitamin E. Based upon these results, Se and vitamin E may play an important role in preventive indication of the oxidative damage associated by acetic acid caused inflammation.
Department of Pathology, Faculty of Medicine, University of Harran, Sanliurfa, 63200, Turkey.
Muharrem
M
Bitiren
Ali Ziya
AZ
Karakilcik
Mustafa
M
Zerin
Ilyas
I
Ozardali
Sehabettin
S
Selek
Yaşar
Y
Nazligül
Abdullah
A
Ozgonul
Davut
D
Musa
Ali
A
Uzunkoy
D019342:Acetic Acid; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002374:Catalase; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000473:pathology; D004150:Dipeptidases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000627:therapeutic use*; D024505:Tocopherols / Q000627:therapeutic use*
D000975:Antioxidants; D002374:Catalase; D009195:Peroxidase; D004150:Dipeptidases; C022449:proline dipeptidase; D012643:Selenium; D019342:Acetic Acid; D024505:Tocopherols