Cell transplantation of adipose tissue-derived stem cells in combination with heparin attenuated acute liver failure in mice.
The effect of adipose tissue-derived stem cells (ASCs) in combination with heparin transplantation on acute liver failure mice with carbon tetrachloride (CCl(4)) injection was investigated. CCl(4) is a well-known hepatotoxin and induces hepatic necrosis. Heparin did not affect the viability of ASCs for at least 24 h. The injection of heparin into the caudal tail vein decreased slightly the activities of the alanine aminotransferase (ALT), asparate aminotransferase (AST), and lactate dehydrogenase (LDH) in plasma. In the transplantation of ASCs (1 x 10(6) cells) group, there was a trend toward decreased activities of all markers. However, four out of six mice died of the lung infarction. In the transplantation of ASCs in combination with heparin group, there was also a trend toward decreased activities of all markers. In addition, all mice survived for at least the duration of the study period. In conclusion, the transplantation of ASCs in combination with heparin was thus found to effectively treat acute liver failure.
Yukawa
Hiroshi
H
Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461-0047, Japan. hiroshiy@med.nagoya-u.ac.jp
Noguchi
Hirofumi
H
Oishi
Koichi
K
Takagi
Soichi
S
Hamaguchi
Michinari
M
Hamajima
Nobuyuki
N
Hayashi
Shuji
S
D000273:Adipose Tissue / Q000166:cytology*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride; D003131:Combined Modality Therapy; D005260:Female; D006493:Heparin / Q000627:therapeutic use*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology
D000925:Anticoagulants; D006493:Heparin; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase