Ostrich involvement in the selection of H5N1 influenza virus possessing mammalian-type amino acids in the PB2 protein.
Amino acids at positions 627 and 701 in the PB2 protein (PB2-627 and PB2-701, respectively) of avian influenza A viruses affect virus replication in some mammalian cells. Highly pathogenic H5N1 influenza viruses possessing mammalian-type PB2-627 were detected during the Qinghai Lake outbreak in 2005 and spread to Europe and Africa. Via a database search, we found a high rate of viral isolates from Ratitae, including ostrich, possessing mammalian-type PB2-627 or -701. Here, we report that H5N1 avian influenza viruses possessing mammalian-type amino acids in PB2-627 or -701 are selected during replication in ostrich cells in vitro and in vivo.
Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. kyoko.tanaka@aioros.ocn.ne.jp
Kyoko
K
Shinya
Akiko
A
Makino
Makoto
M
Ozawa
Jin Hyun
JH
Kim
Yuko
Y
Sakai-Tagawa
Mutsumi
M
Ito
Quynh Mai
QM
Le
Yoshihiro
Y
Kawaoka
D000818:Animals; D002642:Chick Embryo; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry / Q000502:physiology*; D020310:Struthioniformes / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
C502893:PB2 protein, influenza virus; D014764:Viral Proteins