Chromatin flow cytometry identifies changes in epigenetic cell states.
We report flow cytometric protocols that quantitatively display the levels of different histone modifications on the single cell level. This method allows the rapid and simultaneous analysis of the global levels of chromatin marks in combination with other flow cytometric features. We show that chromatin flow cytometry identifies changes in epigenetic marks, such as histone acetylation and methylation, in drug-treated and differentiated mouse embryonic stem cell populations.
Obier
Nadine
N
Institute for Medical Radiation and Cell Research, University of Würzburg, Germany.
Müller
Albrecht M
AM
D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D044127:Epigenesis, Genetic; D005434:Flow Cytometry / Q000379:methods*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D011495:Histone-Lysine N-Methyltransferase / Q000037:antagonists & inhibitors; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D008745:Methylation / Q000187:drug effects; D051379:Mice; D011799:Quinazolines / Q000494:pharmacology
D001381:Azepines; C518299:BIX 01294; D002843:Chromatin; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D011799:Quinazolines; C012589:trichostatin A; D011495:Histone-Lysine N-Methyltransferase