Endocrine disruption and ovarian morphometric responses in rats following exposure to tetradifon.
We have investigated whether exposure to tetradifon causes ovary injuries, disrupts folliculogenesis in rat and whether ovary hormones (estrogen and progesterone) to be affected by this endocrine-active agent. Female rats were exposed orally to a dose of 28.9 mg/kg/day for 6 or 12 weeks. After sacrifice, ovary glands were examined for morphometric changes. The serums were used to determine levels of 17beta-estradiol and progesterone. Results showed no sign of toxicity. However, tetradifon promoted a significant increase in the percentage of atretic follicles in the 12-weeks treated rats. Number and the diameter of mature follicles (tertiary and preovulatory) were markedly diminished together with a reduction of the relative weight of ovaries. Compared with controls, the treated rats exhibited significant reduction in serum 17beta-estradiol and progesterone levels. These results suggest an endocrine disruption by tetradifon which may interfere with ovarian follicles development in rat.
Laboratory of Histology, Medicine Faculty of Sfax, 3029 Sfax, Tunisia. riadh.badraoui@etud.univ-angers.fr
Riadh
R
Badraoui
Nouha B
NB
Abdelmoula
Nozha
N
Feki
Hmed
H
Ben Nasr
Tarek
T
Rebai
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D005496:Follicular Atresia / Q000187:drug effects; D006843:Hydrocarbons, Chlorinated / Q000008:administration & dosage / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D010053:Ovary / Q000187:drug effects* / Q000254:growth & development*; D011374:Progesterone / Q000097:blood; D051381:Rats
D052244:Endocrine Disruptors; D006843:Hydrocarbons, Chlorinated; D011374:Progesterone; D004958:Estradiol; C005807:tetradifon