[Time-order expression of caspase-3 and iNOS in contused human brain tissue].
OBJECTIVE
To investigate the expression of caspase-3 and iNOS in different intervals and to provide evidence for estimation of injury intervals after brain contusion in human.


METHODS
Thirty cases died of serious brain injury were included into the injury groups and 5 cases died of non-brain injury were served as control group. To analyze the changes of caspase-3 and iNOS expression in brain samples at different intervals (2h, 4-8h, 10-14h, 1-2d, 3-5d, 8-11d) by immunohistochemistry and auto-image analysis system.


RESULTS
The level of caspase-3 expression started to increase in 2 hours after brain contusion compared to the control group (P<0.05). The level of caspase-3 expression continued to increase in 1-2 days and maintained high level in 3-5 days compared to the control group (P<0.05), then decreased gradually. There was no statistically significant difference between the expression level of iNOS in 2 hours with the control group (P>0.05). But the expression level of iNOS began to increase in 4-8 hours after brain contusion and reached its maximum in 1-2 days, then decreased. Weak expression of iNOS still could be detected in 8-11 days.


CONCLUSIONS
The expression of caspase-3 and iNOS can be used as effective evidence for human brain contusion interval.
Cai
Hong-Xing
HX
Department of Forensic Medicine, Xuzhou Medical College, Xuzhou 221002, China.
Li
Zhou-Ru
ZR
Cheng
Yan-Bo
YB
Dong
Guo-Kai
GK
Zhong
Jing-Fang
JF
Yin
Wen-Jiang
WJ
D000328:Adult; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism*; D005260:Female; D049429:Forensic Pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D013997:Time Factors; D055815:Young Adult
D012333:RNA, Messenger; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D053148:Caspase 3