[Differential proteomics in glioblastoma].
OBJECTIVE
To establish differential proteomics profiles of glioblastoma cell lines from Chinese, and to provide reference for future basic studies.


METHODS
Total protein was extracted from 3 glioblastoma cell lines, CHG-5, TJ899 and TJ905. After normalization, the total protein was presented by two-dimensional (2D) electrophoresis, scanned and analyzed. Some of the identified protein spots were verified by immunocytochemistry of cell lines and immunohistochemistry of solid tumors. The glia cells were used as the control throughout the study.


RESULTS
A total of 13 differential protein spots were selected, and eventually 10 were identified as unique proteins. These 10 proteins were involved in cytoskeleton forming, cellular metabolism, tumor migration, stress and inflammatory reaction. Immunocytochemistry and immunohistochemistry further confirmed these proteins present in the solid tumors.


CONCLUSIONS
Distinct differential proteomics profiles exist in glioblastoma cell lines and normal glia cells, likely related to the transformation of normal glia to glioma.
Department of Pathology, Department of Neurosurgery, Affiliated Hospital of Medical College of Chinese People's Armed Police Force, Tianjin 300162, China.
Zhan-Kao
ZK
Zhao
Zhong-Min
ZM
Jiang
Xiao-Zhi
XZ
Liu
Li-Sha
LS
Qi
Xiao-Jing
XJ
Qin
D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; C072061:coronin proteins; D002402:Cathepsin D