Influence of obstetric factors on osteogenic potential of umbilical cord-derived mesenchymal stem cells.
Wharton's jelly from the umbilical cord is a noncontroversial source of mesenchymal stem cells (WJMSCs) with high plasticity, proliferation rate and ability to differentiate towards multiple lineages. WJMSCs from different donors have been characterized for their osteogenic potential. Although there is large evidence of WJMSCs plasticity, recently scientific debate has focused on MSCs selection, establishing predictable elements to discriminate the cells with most promising osteoprogenitor cell potential.In the present study a comparative study between the presence of osteoblastic markers and different parameters that pertain to both the newborn and the mother was performed. Umbilical cords were collected after all patients signed the informed consent and local ethical commettee approved the study. Obstetric parameters, including baby's gender and birth weight, mother's age at delivery, gestational stage at parturition and mode of delivery were examined. After characterization and expansion, WJMSCs were analyzed for two osteoblastic markers, alkaline phosphatase (ALP) activity, and the expression level of RUNX-2 transcription factor, and for their ability to deposit mineralized matrix after osteogenic induction.We found that osteoblastic potential was not influenced by baby's gender and mode of delivery. On the contrary, the highest degree of osteoblastic potential has been shown by WJMSCs with RUNX-2 high basal levels, selected from umbilical cords of the heaviest term babies.Even if further evaluation is required, our hypothesis is that our findings may help in selecting the optimal umbilical cord donors and in collecting high potential Wharton's jelly-derived osteoprogenitors efficiently.
Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Italy. letipen@yahoo.it
Letizia
L
Penolazzi
Renata
R
Vecchiatini
Stefania
S
Bignardi
Elisabetta
E
Lambertini
Elena
E
Torreggiani
Alessandro
A
Canella
Tiziana
T
Franceschetti
Giorgio
G
Calura
Fortunato
F
Vesce
Roberta
R
Piva
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis; D001724:Birth Weight; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D000071063:Endoglin; D005260:Female; D005434:Flow Cytometry; D005865:Gestational Age; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D007231:Infant, Newborn; D019012:Integrin beta1 / Q000032:analysis; D008297:Male; D008423:Maternal Age; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000032:analysis; D018800:Thy-1 Antigens / Q000032:analysis; D014470:Umbilical Cord / Q000166:cytology* / Q000378:metabolism; D055815:Young Adult
D015703:Antigens, CD; D050659:Core Binding Factor Alpha 1 Subunit; C064206:ENG protein, human; D000071063:Endoglin; D018960:Hyaluronan Receptors; D019012:Integrin beta1; C493709:RUNX2 protein, human; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens; D000469:Alkaline Phosphatase