4D retrospective black blood trueFISP imaging of mouse heart.
The purpose of this study was to demonstrate the feasibility of steady-state True fast imaging with steady precession (TrueFISP) four-dimensional imaging of mouse heart at high resolution and its efficiency for cardiac volumetry. Three-dimensional cine-imaging of control and hypoxic mice was carried out at 4.7 T without magnetization preparation or ECG-triggering. The k-space lines were acquired with the TrueFISP sequence (pulse repetition time/echo time = 4/2 ms) in a repeated sequential manner. Retrospective reordering of raw data allowed the reconstruction of 10 three-dimensional images per cardiac cycle. The acquisition scheme used an alternating radiofrequency phase and sum-of-square reconstruction method. Black-blood three-dimensional images at around 200 mum resolution were produced without banding artifact throughout the cardiac cycle. High contrast to noise made it possible to estimate cavity volumes during diastole and systole. Right and left ventricular stroke volume was significantly higher in hypoxic mice vs controls (20.2 +/- 2 vs 15.1 +/- 2; P < 0.05, 24.9 +/- 2 vs 20.4 +/- 2; P < 0.05, respectively). In conclusion, four-dimensional black-blood TrueFISP imaging in living mice is a method of choice to investigate cardiac abnormalities in mouse models.
Centre de Résonance Magnétique des Systèmes biologiques, UMR 5536 CNRS/Université Victor Segalen Bordeaux 2, Bordeaux, France. miraux@rmsb.u-bordeaux2.fr
Sylvain
S
Miraux
Guillaume
G
Calmettes
Philippe
P
Massot
William
W
Lefrançois
Elodie
E
Parzy
Bernard
B
Muller
Laurent M
LM
Arsac
Véronique
V
Deschodt-Arsac
Jean-Michel
JM
Franconi
Philippe
P
Diolez
Eric
E
Thiaudière
D000465:Algorithms; D000818:Animals; D055424:Cardiac-Gated Imaging Techniques / Q000379:methods*; D005260:Female; D006321:Heart / Q000033:anatomy & histology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D019028:Magnetic Resonance Imaging, Cine / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012680:Sensitivity and Specificity