Isolation of 4-coumarate Co-A ligase gene promoter from loblolly pine (Pinus taeda) and characterization of tissue-specific activity in transgenic tobacco.
We characterized promoter activity of a phenylpropanoid biosynthetic gene encoding 4-coumarate Co-A ligase (4CL), Pta4Clalpha, from Pinus taeda. Histochemical- and quantitative assays of GUS expression in the vascular tissue were performed using transgenic tobacco plants expressing promoter-GUS reporters. Deletion analysis of the Pta4Clalpha promoter showed that the region -524 to -252, which has two AC elements, controls the high expression levels in ray-parenchyma cells of older tobacco stems. High activity level of the promoter domain of Pta4CLalpha was also detected in the xylem cells under bending stress. DNA-protein complexes were detected in the reactions of the Pta4CLalpha promoter fragments with the nuclear proteins of xylem of P. taeda. The AC elements in the Pta4CLalpha promoter appeared to have individual roles during xylem development that are activated in a coordinated manner in response to stress in transgenic tobacco.
Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA. ayosa@mail.ecc.u-tokyo.ac.jpv
Yuriko
Y
Osakabe
Keishi
K
Osakabe
Vincent L
VL
Chiang
D000222:Adaptation, Physiological / Q000235:genetics; D001483:Base Sequence; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D018744:DNA, Plant; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D041603:Pinus taeda / Q000201:enzymology / Q000235:genetics*; D018547:Plant Stems / Q000166:cytology / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D052584:Xylem / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
D018744:DNA, Plant; D009687:Nuclear Proteins; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase