Gastro-intestinal handling of water and solutes in three species of elasmobranch fish, the white-spotted bamboo shark, Chiloscyllium plagiosum, little skate, Leucoraja erinacea and the clear nose skate Raja eglanteria.
The present study reports aspects of GI tract physiology in the white-spotted bamboo shark, Chiloscyllium plagiosum, little skate, Leucoraja erinacea and the clear nose skate, Raja eglanteria. Plasma and stomach fluid osmolality and solute values were comparable between species, and stomach pH was low in all species (2.2 to 3.4) suggesting these elasmobranchs may maintain a consistently low stomach pH. Intestinal osmolality, pH and ion values were comparable between species, however, some differences in ion values were observed. In particular Ca(2+) (19.67+/-3.65mM) and Mg(2+) (43.99+/-5.11mM) were high in L. erinacea and Mg(2+) was high (130.0+/-39.8mM) in C. palgiosum which may be an indication of drinking. Furthermore, intestinal fluid HCO(3)(-) values were low (8.19+/-2.42 and 8.63+/-1.48mM) in both skates but very high in C. plagiosum (73.3+/-16.3mM) suggesting ingested seawater may be processed by species-specific mechanisms. Urea values from the intestine to the colon dropped precipitously in all species, with the greatest decrease seen in C. plagiosum (426.0+/-8.1 to 0mM). This led to the examination of the molecular expression of both a urea transporter and a Rhesus like ammonia transporter in the intestine, rectal gland and kidney in L. erinacea. Both these transporters were expressed in all tissues; however, expression levels of the Rhesus like ammonia transporter were orders of magnitude higher than the urea transporter in the same tissue. Intestinal flux rates of solutes in L. erinacea were, for the most part, in an inward direction with the notable exception of urea. Colon flux rates of solutes in L. erinacea were all in an outward direction, although absolute rates were considerably lower than the intestine, suggestive of a much tighter epithelia. Results are discussed in the context of the potential role of the GI tract in salt and water, and nitrogen, homeostasis in elasmobranchs.
Department of Biological Sciences, University of Manitoba, Winnipeg, MB, Canada. andersow@cc.umanitoba.ca <andersow@cc.umanitoba.ca>
W Gary
WG
Anderson
Patricia J
PJ
Dasiewicz
Suadi
S
Liban
Calen
C
Ryan
Josi R
JR
Taylor
Martin
M
Grosell
Dirk
D
Weihrauch
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport / Q000235:genetics; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D012623:Seawater; D012754:Sharks / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D012860:Skates, Fish / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014508:Urea / Q000378:metabolism; D014882:Water-Electrolyte Balance; D000096982:Urea Transporters
D000641:Ammonia; D001639:Bicarbonates; D002118:Calcium; D008274:Magnesium; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D014508:Urea; D000096982:Urea Transporters