p53 gene therapy using RNA interference.
p53 gene, discovered almost 35 years ago, keeps the main role in cell cycle control, apoptosis pathways and transcription. p53 gene is found mutated in more than 50% of all human cancers in different locations. Many structures from viral to non viral were designed to incorporate and deliver in appropriate conditions forms of p53 gene or its transcripts, systemically to target tumor cells and to eliminate them through apoptosis or to restore the normal tumor suppressor gene role. Each delivery system presents advantages and low performance in relation to immune system recognition and acceptance. One of the major discoveries in the last years, silencing of RNA, represents a powerful tool for inhibiting post transcriptional control of gene expression. According to several studies, the RNA silencing technology for p53 transcripts together with other carriers or transporters at nano level can be used for creating new therapeutic models. RNA interference for p53 uses different double-stranded (ds) molecules like short interfering (si) RNA and, despite the difficulty of introducing them into mammalian cells due to immune system response, it can be exploited in cancer therapy.
Cancer Institute I.Chiricuta, Cluj Napoca, Romania. ioanaberi@iocn.ro
I
I
Berindan-Neagoe
O
O
Balacescu
C
C
Burz
C
C
Braicu
L
L
Balacescu
O
O
Tudoran
V
V
Cristea
A
A
Irimie
D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D016158:Genes, p53; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008957:Models, Genetic; D009369:Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D034622:RNA Interference / Q000502:physiology*; D014158:Transcription, Genetic