Are anti-Helicobacter pylori urease antibodies involved in atherosclerotic diseases?
OBJECTIVE
The ureB subunit of urease is a major target recognized by the antibodies of Helicobacter pylori-infected patients. The minimal epitope was determined to be an 8-mer peptide (H-SIKEDVQF-OH).


METHODS
The aim of this study was to discover whether this synthetic 8-mer peptide (BK-61A) directly recognizes the anti-ureB subunit antibodies of H. pylori-infected and atherosclerotic patients. To achieve a better presentation of the epitopes to antibodies, a new isocyanuric linker was designed and used for to immobilize the peptides on a cellulose support.


RESULTS
In this study a new peptide synthesis method is presented. Anti-ureB antibodies were evaluated by the dot blot technique in 26 H. pylori-infected donors and the sera of 20 H. pylori-infected patients with atherosclerosis using the 8-mer peptide.


CONCLUSIONS
The results reveal that the BK-61A peptide could be used for diagnosing the presence of anti-ureB antibodies that may be involved in the initiation of atherosclerosis.
Department of Microbiology, Institute of Biology, Jan Kochanowski University, ul Swietokrzyska 15, 25-406 Kielce, Poland. arabski@ujk.kielce.pl
Michał
M
Arabski
Iwona
I
Konieczna
Dariusz
D
Sołowiej
Alicja
A
Rogoń
Beata
B
Kolesińska
Zbigniew
Z
Kamiński
Wiesław
W
Kaca
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D050197:Atherosclerosis / Q000201:enzymology / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D016481:Helicobacter Infections / Q000201:enzymology / Q000276:immunology; D016480:Helicobacter pylori / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014510:Urease / Q000235:genetics / Q000276:immunology*; D055815:Young Adult
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D010455:Peptides; D014510:Urease