Cloning and bioinformatic analysis of lovastatin biosynthesis regulatory gene lovE.
BACKGROUND
Lovastatin is an effective drug for treatment of hyperlipidemia. This study aimed to clone lovastatin biosynthesis regulatory gene lovE and analyze the structure and function of its encoding protein.


METHODS
According to the lovastatin synthase gene sequence from genebank, primers were designed to amplify and clone the lovastatin biosynthesis regulatory gene lovE from Aspergillus terrus genomic DNA. Bioinformatic analysis of lovE and its encoding animo acid sequence was performed through internet resources and software like DNAMAN.


RESULTS
Target fragment lovE, almost 1500 bp in length, was amplified from Aspergillus terrus genomic DNA and the secondary and three-dimensional structures of LovE protein were predicted.


CONCLUSIONS
In the lovastatin biosynthesis process lovE is a regulatory gene and LovE protein is a GAL4-like transcriptional factor.
School of Life Science and Biopharmacology, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.
Xin
X
Huang
Hao-ming
HM
Li
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008148:Lovastatin / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins; D008148:Lovastatin