[Association between the plasma levels of TNF-alpha, IFN-gamma, IL-10, nitric oxide and specific IgG isotypes in the clinical forms of chronic Chagas disease].
Chagas disease is an important chronic parasitic disease that affects around 9-11 million people in Latin America. A combination of parasite and host-related factors are probably responsible for pathogenesis in the chronic phase of the disease. Among the host-related factors, the immunological response is a parameter of special interest. Our aim here was to evaluate the plasma levels of the cytokines interferon gamma, interleukin 10 and tumor necrosis factor alpha and the immunoglobulins total IgG and its subclasses 3 and 4, by means of ELISA, and the levels of nitric oxide by means of the Griess reaction, among individuals who were seropositive for Trypanosoma cruzi, presenting the cardiac, indeterminate and digestive clinical forms of the disease, and among seronegative individuals. The seropositive individuals produced significantly higher levels of total IgG and IgG-3. Individuals with the digestive form presented higher levels of IgG-4 and interleukin 10. However, these individuals presented lower levels of nitric oxide than the controls did. The results suggest that the higher levels of interleukin 10 observed among individuals with the digestive form may contribute towards the higher levels of the specific IgG-4 that were seen.
Laboratório de Imunologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG.
Cristina Wide
CW
Pissetti
Dalmo
D
Correia
Teodoro
T
Braga
Gladstone Eustáquio Lima
GE
Faria
Rafael Faria de
RF
Oliveira
Betânia Maria
BM
Ribeiro
Denise Bertulucci Rocha
DB
Rodrigues
Virmondes
V
Rodrigues
D016022:Case-Control Studies; D014355:Chagas Disease / Q000097:blood* / Q000145:classification; D002908:Chronic Disease; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D009569:Nitric Oxide / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D016207:Cytokines; D007074:Immunoglobulin G; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma