Oxidative stress in rat hippocampus caused by pilocarpine-induced seizures is reversed by buspirone.
Temporal lobe epilepsy is the most common form of epilepsy in humans. Oxidative stress is a mechanism of cell death induced by seizures. Buspirone presents anxyolitic and antidepressant effects due to their ability to stimulate 5-HT(1A) receptor. We studied the buspirone effects on oxidative stress in rat hippocampus after seizures and status epilepticus (SE) induced by pilocarpine. In pilocarpine group there was a significant increase in lipid peroxidation and nitrite levels. However, no alteration was observed in superoxide dismutase and catalase activities. Buspirone pretreatment produces significantly reduction of the lipid peroxidation level (60%) and nitrite content (44%) as well as increased the superoxide dismutase (47%) and catalase (40%) activities in rat hippocampus after seizures, when compared with the pilocarpine group. The intraperitoneal injection of buspirone prior to pilocarpine suppressed the behavioral seizure occurrence. According to our results, the oxidative stress is present during seizures. Buspirone exerted anticonvulsant effects associated with the inhibition of the development of oxidative stress. These results suggest a therapeutic use potential of buspirone in epilepsy treatment.
de Freitas
Rizângela Lyne Mendes
RL
Course of Pharmacy of Catholic Faculty Rainha do Sertão, Quixada, Ceara, CE, Brazil. rivelilson@ufpi.br
Santos
Itala Mônica de Sales
IM
de Souza
Geane Felix
GF
Tomé
Adriana da Rocha
Ada R
Saldanha
Gláucio Barros
GB
de Freitas
Rivelilson Mendes
RM
D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage / Q000494:pharmacology; D002065:Buspirone / Q000008:administration & dosage / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology*; D017366:Serotonin Receptor Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D013226:Status Epilepticus / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D000927:Anticonvulsants; D009573:Nitrites; D017366:Serotonin Receptor Agonists; D010862:Pilocarpine; D002374:Catalase; D013482:Superoxide Dismutase; D002065:Buspirone