[Preliminary study on cardiac allograft rejection in mice by Extractum trametes robiniophila murr].
OBJECTIVE
To study the effect of Extractum trametes robiniophila murr on cardiac allograft rejection in mice.


METHODS
All abdominal heterotopic heart transplantation models were divided into three groups as follows: (A) Extractum trametes robiniophila murr group. (B) Rejection group. (C) Isograft group. In each group, mean survival times (MST) of transplanted hearts and their pathologic histological changes at postoperative fifth day were observed. With fluoroimmunoassay, granzyme B and CD8(+) expressions were examined.


RESULTS
The MST of heart allografts in group A were (6.38 +/- 0.69) d, significantly shorter than that of group B [(8.31 +/- 0.59) d] (P < 0.01). In group A, acute rejection was present in advance; transplanted hearts were seriously damaged into acute rejection pathological grade 3, and CD8(+) T lymphocytes infiltrated diffusely and the expression of granzyme B increased significantly as compared with other groups.


CONCLUSIONS
Exclusive application of Extractum trametes robiniophila murr can promote the acute rejection of graft in early phase of postoperation, and the mechanism may be the promoted proliferation and infiltration of CD8(+) T lymphocytes and the increased expression of granzyme B.
Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Qi
Q
Cheng
Peng
P
Zhu
Dan
D
Li
Zhi-Yuan
ZY
Huang
Hui-Fang
HF
Liang
Yi-Fa
YF
Chen
Xiao-Ping
XP
Chen
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000009:adverse effects*; D005260:Female; D006084:Graft Rejection / Q000139:chemically induced*; D053804:Granzymes / Q000378:metabolism; D016027:Heart Transplantation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000201:enzymology / Q000276:immunology; D011182:Postoperative Care
D004365:Drugs, Chinese Herbal; D053804:Granzymes