Photophysical characterization of oligopyrene modules for DNA-based nanosystems.
The photophysics of free pyrenedicarboxamide (Py-DCA) in solution as well as of single-stranded and double-stranded oligonucleotides (ss and ds ONs) containing 1-7 pyrene building blocks per strand were studied by steady-state and time-resolved fluorescence spectroscopy. It was found that the fluorescence quantum yield Phi(F) of free Py-DCA chromophore in solution is rather high (Phi(F) = 0.44). However, after incorporation of the chromophore into a ss ON the monomeric chromophore fluorescence is quenched more than 40-fold due to electron-transfer reactions with ON bases. An increase of the number n of neighboring pyrenes in an ON results in Phi(F) growth up to 0.25 at n = 6. Starting from n = 2, all fluorescence belongs mainly to excimer formed by pyrene chromophores. Sections composed of multiple pyrenes may be considered as robust functional entities that may serve as independent modules in DNA-based, functional nano-architectures.
B.I. Stepanov's Institute of Physics, National Academy of Sciences of Belarus, Nezavisimosti ave. 68, 220072, Minsk, Belarus.
Victor A
VA
Galievsky
Vladimir L
VL
Malinovskii
Alexander S
AS
Stasheuski
Florent
F
Samain
Klaas A
KA
Zachariasse
Robert
R
Häner
Vladimir S
VS
Chirvony
D000042:Absorption; D000577:Amides / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D000577:Amides; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D011721:Pyrenes; D004247:DNA; C030984:pyrene