Expression of TIP30 tumor suppressor gene is down-regulated in human colorectal carcinoma.
OBJECTIVE
Human TIP30 was initially identified as a candidate metastasis suppressor gene whose expression was down-regulated in human liver, lung, breast, and prostate cancers, and recently the role of this gene was examined in colorectal cancer. The aim of this study was to determine the level of TIP30 expression in colorectal carcinoma (CRC).


RESULTS
TIP30 protein levels were lower in colorectal carcinomas compared to normal tissue from the control group (P < 0.001). The frequencies of hypermethylation of TIP30 in tumor were 36%, while there was no aberrant methylation in paired adjacent non-tumor tissue. A statistically significant inverse association was found between TIP30 methylation status and expression of the TIP30 protein in tumor tissues (P = 0.006). Somatic missense mutations in the TIP30 gene were identified in human CRC tissue specimens.


CONCLUSIONS
Our results demonstrate that promoter methylation is involved in the decreased expression of TIP30 tumor suppressor gene in human colorectal carcinoma.
Chen
Xiaobing
X
Department of Internal Medicine-Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
Cao
Xinguang
X
Dong
Wenjie
W
Luo
Suxia
S
Suo
Zhenhe
Z
Jin
Yinchun
Y
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D014157:Transcription Factors; D000123:Acetyltransferases; C111246:HTATIP2 protein, human