Expression of Breast Cancer Resistance Protein (BCRP-1) in canine mammary adenocarcinomas and adenomas.
BACKGROUND
Among the recognized factors which induce multi-drug resistance, an increased activity of proteins belonging to the ATP-binding casette family, including breast cancer resistance protein 1 (BCRP-1), is regarded as the most important. Localization and intensity of BCRP-1 expression was evaluated in mammary adenocarcinomas and adenomas in dogs. The obtained results were compared to the grade of malignancy (G) of the tumours.


METHODS
Materials for the study were sampled in the course of surgery from 54 dogs, of various breeds, aged 6 to 16 years (36 cases of mammary adenocarcinoma and 18 cases of mammary adenoma). The tumours were histopathologically verified and immunohistochemical reactions were performed to evaluate expression of BCRP-1. The microscopic patterns were photographed and subjected to computer-assisted analysis taking advantage of MultiScanBase Ver. 14.02 software.


RESULTS
Expression of BCRP-1 was detected in over 85% of adenocarcinomas and almost 28% of adenomas. Samples of tumours with a higher grade of malignancy demonstrated an increased expression of BCRP-1. The two variables manifested a moderate positive correlation (r=0.35; p<0.05).


CONCLUSIONS
The results point to a role of BCRP-1 protein in biology of tumour cells in dogs.
Nowak
Marcin
M
Department of Pathological Anatomy, Pathophysiology, Microbiology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. marcin.nowak@up.wroc.pl
Madej
Janusz A
JA
Dziegiel
Piotr
P
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D000236:Adenoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002452:Cell Count / Q000662:veterinary; D004285:Dogs; D005260:Female; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques / Q000662:veterinary; D015674:Mammary Neoplasms, Animal / Q000378:metabolism* / Q000473:pathology / Q000601:surgery
D018528:ATP-Binding Cassette Transporters; D014408:Biomarkers, Tumor