[Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production].
Candida glycerinogenes WL2002-5 (C.g) is an important industrial strain for glycerol production. To further improve glycerol production, we reconstructed a binary vector pCAM3300-zeocin-CgGPD1, introduced it to Agrobacterium tumefaciens LBA4404 by electroporation, and then transformed the T-DNA harboring the CgGPD1 to Candida glycerinogenes by Agrobacterium tumefaciens-mediated transformation (ATMT). After 96 h fermentation with glucose as the substrate, we screened a transformant named C.g-G8 with high glycerol production. Compared with the wild strain, the glucose consumption rate of C.g-G8 and the glycerol production were 12.97% and 18.06% higher, respectively. During the fermentation, the activity of glycerol-3-phosphate dehydrogenase of C.g-G8 was 27.55% higher than that of the wild strain. The recombinant Candida glycerinogenes with high glycerol production was successful constructed by ATMT method.
The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Ailing
A
Liu
Zhiming
Z
Rao
Zheng
Z
Ma
Bin
B
Zhuge
Huiying
H
Fang
Jian
J
Zhuge
D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics*; D002175:Candida / Q000235:genetics* / Q000378:metabolism; D018274:Electroporation; D005285:Fermentation; D005990:Glycerol / Q000032:analysis* / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D005993:Glycerolphosphate Dehydrogenase; D005990:Glycerol