Concurrent extraction of proteins and RNA from cell-laden hydrogel scaffold free of polysaccharide interference.
Concurrent extractions of proteins and RNA from cell-laden scaffolds would be of great help to biomaterialists and tissue engineers. Here we describe a procedure to extract proteins from the discard solution generated during the RNA isolation from polysaccharide-rich hydrogel scaffolds. This approach allows to obtain proteins and RNA from same sample while eliminating the polysaccharide interference.
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, Singapore 637457, Republic of Singapore.
Chunming
C
Wang
Xin
X
Li
Yongchang
Y
Yao
Dong-An
DA
Wang
D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D011134:Polysaccharides; D011506:Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012313:RNA