Imaging nervous system activity.
This unit describes methods for loading ion- and voltage-sensitive dyes into neurons, with a particular focus on the spinal cord as a model system. In addition, we describe the use of these dyes to visualize neural activity. Although the protocols described here concern spinal networks in culture or an intact in vitro preparation, they can be, and have been, widely used in other parts of the nervous system.
Fields
Douglas R
DR
Section on Nervous System Development and Plasticity, National Institute of Child Health and Human Development (NICHD), NIH, Bethesda, Maryland, USA.
Shneider
Neil
N
Mentis
George Z
GZ
O'Donovan
Michael J
MJ
D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009415:Nerve Net / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009482:Neurophysiology / Q000379:methods*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes