[Influence factors of the separation of steroids using oil-in-water microemulsion liquid chromatography].
The effect of the varying operating parameters on the separation of steroids was studied by oil-in-water microemulsion liquid chromatography (MELC). The parameters included the surfactant concentration, the type of oil phase, the nature of organic solvent additives, and the pore size of the stationary-phase. The optimized conditions for the separation of 6 steroids were as follows: a Venusil ASB C18 (T) column (5 microm, 30 nm, 250 mm x 4.6 mm) was used at 35 degrees C, the microemulsion mobile phase consisted of 30 g/L sodium dodecylsulfate (SDS), 0.8% (w/w) n-octane, 6.6% (w/w) n-butanol. The optimized method can be used for the separation, identification and simultaneous determination of steroids in bulk drugs and in pharmaceutical dose forms.
Guangdong College of Pharmacy, Guangzhou 510006, China. godhs@tom.com
Ning
N
Li
Xuanzhu
X
Hou
Wen
W
Yang
Guangliang
G
Huang
Xiujin
X
Ye
D002853:Chromatography, Liquid / Q000379:methods*; D003348:Cortisone / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004655:Emulsions; D011241:Prednisone / Q000302:isolation & purification*; D011374:Progesterone / Q000302:isolation & purification*; D013256:Steroids / Q000737:chemistry*
D004655:Emulsions; D013256:Steroids; D011374:Progesterone; D003348:Cortisone; D011241:Prednisone