Attachment of stem cells to scaffold particles for intra-cerebral transplantation.
Cell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin needle. However, this does not provide cells with a support system that would allow tissue regeneration. Scaffold particles are needed to provide structural support to cells to form de novo tissue. In this 16-d protocol, we describe the generation and functionalization of poly (D,L-lactic-co-glycolic) acid (PLGA) particles to enhance cell attachment, the attachment procedure to avoid clumping and aggregation of cells and particles, and their preparation for intra-cerebral injection through a thin needle. Although the stem cell-scaffold transplantation is more complicated and labor-intensive than cell suspensions, it affords de novo tissue generation inside the brain and hence provides a significant step forward in traumatic brain repair.
Bible
Ellen
E
Department of Neuroscience, Institute of Psychiatry, Kings College London, London, UK.
Chau
David Y S
DY
Alexander
Morgan R
MR
Price
Jack
J
Shakesheff
Kevin M
KM
Modo
Michel
M
D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D054022:Cerebrum / Q000166:cytology* / Q000601:surgery; D005353:Fibronectins / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D013499:Surface Properties; D054457:Tissue Scaffolds
D005353:Fibronectins