Centrosomal localization of Diversin and its relevance to Wnt signaling.
Wnt pathways regulate many developmental processes, including cell-fate specification, cell polarity, and cell movements during morphogenesis. The subcellular distribution of pathway mediators in specific cellular compartments might be crucial for the selection of pathway targets and signaling specificity. We find that the ankyrin-repeat protein Diversin, which functions in different Wnt signaling branches, localizes to the centrosome in Xenopus ectoderm and mammalian cells. Upon stimulation with Wnt ligands, the centrosomal distribution of Diversin is transformed into punctate cortical localization. Also, Diversin was recruited by Frizzled receptors to non-homogeneous Dishevelled-containing cortical patches. Importantly, Diversin deletion constructs, which did not localize to the centrosome, failed to efficiently antagonize Wnt signaling. Furthermore, a C-terminal construct that interfered with Diversin localization inhibited Diversin-mediated beta-catenin degradation. These observations suggest that the centrosomal localization of Diversin is crucial for its function in Wnt signaling.
Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Keiji
K
Itoh
Andreas
A
Jenny
Marek
M
Mlodzik
Sergei Y
SY
Sokol
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism*; D000072261:Dishevelled Proteins; D004475:Ectoderm / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism*; D014981:Xenopus / Q000378:metabolism*; D029867:Xenopus Proteins / Q000737:chemistry / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
C464904:ANKRD6 protein, Xenopus; D048868:Adaptor Proteins, Signal Transducing; C464903:Ankrd6 protein, mouse; D003598:Cytoskeletal Proteins; D000072261:Dishevelled Proteins; C113865:FZD8 protein, Xenopus; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D011956:Receptors, Cell Surface; D051153:Wnt Proteins; D029867:Xenopus Proteins; D051176:beta Catenin