[The influence of Namalwa cells on inhibition of HSV-1 replication].
The main immunological elements that control infection with herpes simplex virus type 1 include interferon, NK cells, and specific T and B cells. The aim of this study was to determine the influence of Namalwa cells (B cell immortalized by EBV) which produce IFN alpha in large amount on inhibition of HSV-1 replication in CV-1 and MDBK cells. Inhibition of HSV-1 replication was measured by MTT assay. Addition Namalwa cells to CV-1 or MDBK cells infected HSV-1 inhibited virus replication. Degree of inhibition was connected with amount of added cells and with time of addition (before infection or after infection). The highest inhibition of HSV-1 replication showed Namalwa cells added one hour before infection (72% for CV-1 infected HSV-1 and 68% for MDBK infected HSV-1).
Zakład Wirusologii NIZP-PZH w Warszawie. mrechnio@pzh.gov.pl
Magdalena
M
Wieczorek
Bogumiła
B
Litwińska
D001402:B-Lymphocytes / Q000276:immunology*; D002051:Burkitt Lymphoma / Q000276:immunology / Q000821:virology; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D016898:Interferon-alpha / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014779:Virus Replication / Q000276:immunology*
D016898:Interferon-alpha