Different expression of mimecan as a marker for differential diagnosis between NSCLC and SCLC.
Mimecan mRNA was present in a limited number of mouse and human tissues, however, abundant mimecan mRNA was observed in the lung tissue. Therefore, we hypothesize that mimecan could serve as a biomarker for differentiating various histological types of lung cancers. In humans, the mimecan mRNA was found most abundant in ovary and less abundant in lung by using Northern blot analysis. Moreover, the mimecan was expressed strongly in the epithelial cells of the bronchial wall and weaker in the epithelial cells of the alveolar sacs by in situ hybridization and immunohistochemical analysis. Furthermore, the mimecan immunoreactivity was found in 103 (97.2%) of 106 non-small cell lung cancers (NSCLCs). Nevertheless, a large majority of small cell lung cancers (SCLCs) (50/56, 89.3%) showed negative immunoreactivity to mimecan polyclonal antibody. A significant difference of mimecan immunoreactivity was found between NSCLC and SCLC (P<0.00001). This is the first study showing that mimecan could serve as an excellent pathological biomarker to distinguish NSCLCs from SCLCs.
Zheng
Cui-Xia
CX
The Ninth Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, PR China. zcx9566@163.com
Zhao
Shuang-Xia
SX
Wang
Ping
P
Yu
Hui-Min
HM
Wang
Cao-Fu
CF
Han
Bing
B
Su
Bin
B
Xiang
Yi
Y
Li
Xue-Song
XS
Li
Sheng-Xian
SX
Ma
Qin-Yun
QY
Zhang
Rong-Xin
RX
Wan
Huan-Ying
HY
Song
Huai-Dong
HD
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D011379:Prognosis; D055752:Small Cell Lung Carcinoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism
D014408:Biomarkers, Tumor; D036341:Intercellular Signaling Peptides and Proteins; C063488:OGN protein, human