[Investigation on distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai].
OBJECTIVE
To explore the distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai.


METHODS
Nested polymerase chain reaction (nPCR) was used for amplification of S gene and C gene of HBV from sera carried by Tibetan chronic HBV carrier in Tongde, Qinghai, then the HBV DNA positive products were sequenced by direct sequencing. Genotype and serotype were identified by analysis of sequence result.


RESULTS
271, which come from 311 sera samples with positive HBsAg randomly selected from natural community, were amplified and sequenced in both S gene and C gene successfully, 10 (3.7%), 261 (96.3%) out of them were identified as genotype C, recombinant between genotypes C and D respectively; 259 (95.6%), 10 (3.7%), 2 (0.7%) belonged to serotype ayw2, adr, adw2 respectively.


CONCLUSIONS
The recombinant between genotypes C and D was the main genotype in Tibetan chronic carrier with hepatitis Bin Tongde, Qinghai; the serotype of this areas was consisted largely of ayw2.
Zang
Jie
J
Department of Epidemiology and Healthy Statistics, Hebei Medical University, Shijiazhuang 050017, China.
Jiang
Shuang-Ying
SY
Wang
Xue-Wen
XW
Wang
Feng
F
Bian
Tao
T
Mu
Zhen-Yun
ZY
Zhang
Yong
Y
D000293:Adolescent; D000328:Adult; D002681:China; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood; D006509:Hepatitis B / Q000276:immunology / Q000821:virology*; D006512:Hepatitis B Core Antigens / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D006508:Hepatitis Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens