Phenotypic modulation in Mycobacterium tuberculosis infected neutrophil during tuberculosis.
OBJECTIVE
Polymorphonuclear leucocytes (PMN) or neutrophils infiltrate to the inflammatory sites and phagocytose mycobacteria thereby inhibiting the bacillary spread initially until the accumulated macrophages get activated. The present study was carried out to highlight the interaction of neutrophils with the two clinical isolates (S7 and S10) of Mycobacterium tuberculosis and the subsequent morphological changes.


METHODS
Dextran purified neutrophils from normal and TB patients infected with M. tuberculosis isolates were cultured for 3 and 18 h time points. At the end of termination, the cell surface expression of CD16, CD69, CXCR2 and induction of apoptosis were analyzed using flow cytometry. Cytokines and chemokines were estimated in supernatants by ELISA.


RESULTS
All infected PMN showed decrease in CD16 at both time points in normals while at 18 h in TB group. Interestingly, CD69 expression was significantly high at early time point in TB-PMN compared to normals. The high expression of CXCR2 was sustained in infected TB-PMN at both the time points. S7 and S10 infected neutrophils showed high phagocytic indices compared to H37Rv in both the groups. A significant increase in apoptosis was observed at both the time points in infected TB-PMN but only at 18 h in normals. Increased pro-inflammatory cytokine (TNF-alpha) and chemokine (IL-8) response was observed in infected neutrophils at 3 h in both the groups.


CONCLUSIONS
This study demonstrates the varying degree of modulation of neutrophil functions in both the groups. TB-PMN was more competent in amplifying the innate immune response and conferring protection at the early phase of infection. However, the response was not strain specific in either of these groups.
Department of Immunology, Tuberculosis Research Centre (ICMR), Chennai, India.
Supriya
S
Pokkali
Priya
P
Rajavelu
R
R
Sudhakar
Sulochana D
SD
Das
D000328:Adult; D001500:BCG Vaccine; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D010587:Phagocytosis; D010641:Phenotype; D017452:Receptors, IgG / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control; D055815:Young Adult
D001500:BCG Vaccine; D016207:Cytokines; D017452:Receptors, IgG