Screening of a large global Aspergillus fumigatus species complex collection by using a species-specific microsphere-based Luminex assay.
A microsphere-based Luminex assay was developed and validated for rapid identification of Aspergillus fumigatus from the other species within the A. fumigatus species complex (section Fumigati). This molecular tool was then employed to screen 499 clinical A. fumigatus species complex isolates collected from multiple medical centers throughout the world with results demonstrating the exclusive presence of A. fumigatus.
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Kizee A
KA
Etienne
Lalitha
L
Gade
Shawn R
SR
Lockhart
Daniel J
DJ
Diekema
Shawn A
SA
Messer
Michael A
MA
Pfaller
S Arunmozhi
SA
Balajee
D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D006801:Humans; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D004271:DNA, Fungal