Cross-linking enhances deposition of human endothelial progenitor cells in the rat heart after intracoronary transplantation.
Transplantation of human endothelial progenitor cells (hEPCs) may improve vascularization and left ventricular function after myocardial infarction. The scope of this study was to explore, whether cross-linking of EPCs may enhance the deposition of cells in the rat heart after clinical-like, intracoronary transplantation. To this end, (111)In-oxinate-labeled hEPCs were infused by a minimally invasive technique into the coronary arteries of immunosuppressed Wistar rats under control conditions and after ischemia/reperfusion. In a second set of experiments hEPCs were treated with phytohemagglutinin to create small cell clusters prior to transplantation. Continous three-dimensional HiSPECT images for 1 h and after 48 h revealed that cell deposition was significantly higher when hEPCs were cross-linked. Therefore, cross-linking of hEPCs may provide a promising approach to enhance the number of trapped cells also in a clinical setting.
Burghoff
Sandra
S
Institute for Cardiovascular Physiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany. sandra.burghoff@uni-duesseldorf.de
Ding
Zhaoping
Z
Blaszczyk
Angelika
A
Wirrwar
Andreas
A
Buchholz
Doris
D
Müller
Hans-Wilhelm
HW
Schrader
Jürgen
J
D000818:Animals; D002404:Catheterization; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D007261:Infusions, Intra-Arterial / Q000379:methods; D008297:Male; D009203:Myocardial Infarction / Q000628:therapy; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000628:therapy; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015899:Tomography, Emission-Computed, Single-Photon; D016896:Treatment Outcome
D010835:Phytohemagglutinins