High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample).
Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch-Str. 41, D-48149 Münster, Germany. mellmann@uni-muenster.de
A
A
Mellmann
F
F
Bimet
C
C
Bizet
A D
AD
Borovskaya
R R
RR
Drake
U
U
Eigner
A M
AM
Fahr
Y
Y
He
E N
EN
Ilina
M
M
Kostrzewa
T
T
Maier
L
L
Mancinelli
W
W
Moussaoui
G
G
Prévost
L
L
Putignani
C L
CL
Seachord
Y W
YW
Tang
D
D
Harmsen
D001420:Bacteria, Aerobic / Q000737:chemistry* / Q000145:classification*; D001424:Bacterial Infections / Q000175:diagnosis*; D003951:Diagnostic Errors / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*