Presence of structural homologs of ubiquitin in haloalkaliphilic Archaea.
Ubiquitin, a protein widely conserved in eukaryotes, is involved in many cellular processes, including proteolysis. While sequences encoding ubiquitin-like proteins have not been identified in prokaryotic genomes sequenced so far, they have revealed the presence of structural and functional homologs of ubiquitin in Bacteria and Archaea. This work describes the amplification and proteomic analysis of a 400-bp DNA fragment from the haloalkaliphilic archaeon Natrialba magadii. The encoded polypeptide, P400, displayed structural homology to ubiquitin-like proteins such as those of the ThiS family and Urm1. Expression of the P400 DNA sequence in Escherichia coli cells yielded a recombinant polypeptide that reacted with anti-ubiquitin antibodies. In addition, a putative open reading frame encoding P400 was identified in the recently sequenced genome of N. magadii. Together, these results evidence the presence in Archaea of structural homologs of ubiquitin- related proteins.
Nercessian
Débora
D
Institute of Biological Research, Faculty of Exact and Natural Sciences, National University of Mar del Plata and CONICET, Mar del Plata, Argentina. dnercess@mdp.edu.ar
Marino Buslje
Cristina
C
Ordóñez
María V
MV
De Castro
Rosana E
RE
Conde
Rubén D
RD
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D019843:Archaeal Proteins / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006216:Halobacteriaceae / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D040901:Proteomics; D017386:Sequence Homology, Amino Acid; D025801:Ubiquitin / Q000235:genetics* / Q000276:immunology
D000906:Antibodies; D019843:Archaeal Proteins; D025801:Ubiquitin