Characterization of a dammarenediol synthase in Centella asiatica (L.) Urban.
To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative beta-amyrin synthase [Plant Cell Rep, 24:304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C. asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons.
Kim
Ok Tae
OT
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-871, Republic of Korea. kimot99@hanmail.net
Lee
Jei Wan
JW
Bang
Kyong Hwan
KH
Kim
Young Chang
YC
Hyun
Dong Yun
DY
Cha
Seon Woo
SW
Choi
Yong Eui
YE
Jin
Mei Lan
ML
Hwang
Baik
B
D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D000595:Amino Acid Sequence; D028041:Centella / Q000201:enzymology* / Q000235:genetics; D005690:Galactose; D015870:Gene Expression; D017343:Genes, Plant; D019751:Intramolecular Transferases; D009154:Mutation; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012503:Saponins / Q000096:biosynthesis* / Q000235:genetics; D017385:Sequence Homology; D014315:Triterpenes
D010940:Plant Proteins; D012503:Saponins; D014315:Triterpenes; C106188:dammarenediol; D019883:Alkyl and Aryl Transferases; D019751:Intramolecular Transferases; C021924:lanosterol synthase; D005690:Galactose