Cell growth-promoting activity of fluid from eye sacs of the bubble-eye goldfish (Carassius auratus).
The growth-promoting effects of fish body fluids, such as serum and embryonic extract, on fish cell cultures have been widely demonstrated. The bubble-eye variety of aquarium goldfish is characterized as having a large sac filled with fluid (sac fluid) under each eye. These sacs are believed to contain lymph, which is similar in composition to serum or blood plasma. In order to test whether the sac fluid can be used as an additive for fetal bovine serum (FBS) in growth factor supplements, we compared cell growth in media containing FBS together with different concentrations of sac fluid. A dose-dependent growth-promotion effect was observed in early passage caudal fin cells from both medaka and zebrafish. Cell-growth promotion was also confirmed in early passage medaka blastula cells and in a zebrafish embryonic cell line (ZF4). Replacement of the fluid in the eye sacs of bubble-eyes occurs within a couple of months after the sac fluid has been harvested, and the cell-growth promoting activity of the new fluid is similar to that of the fluid that was tapped initially. These findings suggest that sac fluid can be used as a growth-promoting supplement for fish cell culture. Importantly, the ability of the goldfish to replace the fluid combined with the fact that equipotent fluid can be repeatedly harvested from the eye sacs means that a sustainable source of the fluid can be obtained without needing to sacrifice the fish.
Sawatari
Etsuko
E
Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan.
Hashimoto
Hisashi
H
Matsumura
Takaharu
T
Iwata
Yasuhiro
Y
Yamamoto
Naoki
N
Yokoyama
Yoshihiro
Y
Wakamatsu
Yuko
Y
D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005121:Extremities / Q000502:physiology; D006054:Goldfish / Q000502:physiology*; D009990:Oryzias; D015027:Zebrafish