[FTIR spectroscopic study on gallbladder carcinoma cell and nucleus].
The aim of the present research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared spectra) of the cultured carcinoma cell and nucleus with FTIR spectroscopy, and investigating the special spectral features of the carcinoma cell and nucleus compared with the carcinoma tissues. In this paper, the gallbladder carcinoma tissues confirmed by histology were measured using a Nicolet Magna 5700-II FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gallbladder carcinoma cell (GBC-SD) and nucleus were centrifuged to provide a small pellet of cell and nucleus for FTIR analysis. The cell and nucleus pellet was then placed on the OMNIC sampler. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cell and nucleus (GBC-SD) and that of the corresponding gallbladder tissues. Several infrared spectral features of the carcinoma cell and nucleus were obtained. All the results suggest that the spectral features of the carcinoma cell and nucleus can be well reflected by that of the carcinoma tissue, though the later is more complicated, which might originate from the intrinsic complexity of the tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. sunxy@mail.xjtu.edu.cn
Xue-Jun
XJ
Sun
Feng-Lei
FL
Sun
Jun-Kai
JK
Du
Qing-Hua
QH
Pan
Yuan-Fu
YF
Zhang
Yi-Zhuang
YZ
Xu
Jing-Sen
JS
Shi
Jin-Guang
JG
Wu
D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry* / Q000473:pathology*; D002498:Centrifugation; D005706:Gallbladder Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared