A scanning peptide array approach uncovers association sites within the JNK/beta arrestin signalling complex.
Beta arrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/beta arrestin signalling complex. We show that beta arrestin 1 and beta arrestin 2 associate with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known 'common docking' motif. In the N-domain and C-terminus of beta arrestin 1 and beta arrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the beta arrestin 1 and beta arrestin 2 we identify a novel MKK4 docking site.
Li
Xiang
X
Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom.
MacLeod
Ruth
R
Dunlop
Allan J
AJ
Edwards
Helen V
HV
Advant
Noopur
N
Gibson
Lucien C D
LC
Devine
Nicola M
NM
Brown
Kim M
KM
Adams
David R
DR
Houslay
Miles D
MD
Baillie
George S
GS
D000595:Amino Acid Sequence; D000818:Animals; D019390:Arrestins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D048670:MAP Kinase Kinase 4 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology*; D048057:Mitogen-Activated Protein Kinase 10 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000071557:beta-Arrestins
D019390:Arrestins; D019151:Peptide Library; D010455:Peptides; D011993:Recombinant Fusion Proteins; D000071557:beta-Arrestins; D048057:Mitogen-Activated Protein Kinase 10; D048670:MAP Kinase Kinase 4