[Comparison of low temperature- induced proteins in tillering node of winter wheat cultivars with different cold resistance].
By using two-dimensional electrophoresis and mass spectrometry techniques, the proteomes in the tillering node of cold-resistant winter wheat Dongnongdongmai 1 and cold-susceptible winter wheat Jimai 22 before and after low temperature treatment were comparatively analyzed. It was observed that within the range of pH 4-7, there was a significant difference in the expression level of 55 protein spots in the protein profiles of tillering node before and after low temperature treatment, and 47 of the 55 protein spots were expressed in the two cultivars. After low temperature treatment, the abundance value of 23 protein spots increased, with the absolute abundance being higher in Dongnongdongmai 1 than in Jimai 22, while that of 7 protein spots decreased, with the absolute abundance being lower in Dongnongdongmai 1 than in Jimai 22. The expression level of 8 protein spots specific in Dongnongdongmai 1 was up-regulated. In all of the up-regulated proteins, their expression level in Dongnongdongmai 1 was 2.1 - 16.5 times as high as that in Jimai 22, among which, 10 protein spots had an expression level of 4 times high. In the down-regulated proteins, their expression level in Dongnongdongmai 1 was 0.1-0.4 times as high as that in Jimai 22. The differential protein spots were detected by mass spectrometry, and identified by compared with retrieval database. A total of 51 protein spots fingerprints were obtained, among which, 15.7% were of stress protein, 27.5% were of metabolism-related protein, 19.6% were signaling molecules, 9.8% were of unknown protein, and other proteins took up 27.4%. These variously expressed proteins might play an important role in the cold resistance of Dongnongdongmai 1.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China. yujing1981@126.com
Jing
J
Yu
Lin
L
Zhang
Jing
J
Cang
Zai-Bin
ZB
Hao
Yang
Y
Yang
Zhuo-Fu
ZF
Li
D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D014908:Triticum / Q000145:classification / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome