P68 RNA helicase is a nucleocytoplasmic shuttling protein.
P68 RNA helicase is a prototypical DEAD box RNA helicase. The protein plays a very important role in early organ development and maturation. Consistent with the function of the protein in transcriptional regulation and pre-mRNA splicing, p68 was found to predominately localize in the cell nucleus. However, recent experiments demonstrate a transient cytoplasmic localization of the protein. We report here that p68 shuttles between the nucleus and the cytoplasm. The nucleocytoplasmic shuttling of p68 is mediated by two nuclear localization signal and two nuclear exporting signal sequence elements. Our experiments reveal that p68 shuttles via a classical RanGTPase-dependent pathway.
Department of Biology, Georgia State University, Atlanta, GA 30303, USA.
Haizhen
H
Wang
Xueliang
X
Gao
Yun
Y
Huang
Jenny
J
Yang
Zhi-Ren
ZR
Liu
D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology* / Q000235:genetics*; D003593:Cytoplasm / Q000201:enzymology* / Q000235:genetics*; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D049790:Nuclear Export Signals / Q000235:genetics; D019913:Nuclear Localization Signals / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D020931:ran GTP-Binding Protein / Q000235:genetics / Q000378:metabolism
D049790:Nuclear Export Signals; D019913:Nuclear Localization Signals; C102460:Ran protein, mouse; D053487:DEAD-box RNA Helicases; D020931:ran GTP-Binding Protein