Comparison of the effects of low intra-abdominal pressure and pentoxifylline on oxidative stress during CO2 pneumoperitoneum in rabbits.
BACKGROUND
The aim of this study was to investigate the effect of low-pressure pneumoperitoneum and pentoxifylline, a methylxanthine derivative, in the prevention of injury caused by free oxygen radicals generated during CO(2 )pneumoperitoneum.


METHODS
Twenty-eight rabbits were allocated randomly to 4 groups. Control group rabbits (group 1) were subjected to anesthesia for 60 min; group 2 and 3 animals were subjected to a CO(2) pneumoperitoneum (15 or 7 mm Hg); and group 4 rabbits received 50 mg/kg pentoxifylline, followed by a 15-mm-Hg pneumoperitoneum. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), lipid hydroperoxide, glutathione reductase and total antioxidant status were measured.


RESULTS
Compared with group 1, a significant increase in lipid hydroperoxide levels at the end of the pneumoperitoneum and 30 min after deflation and a significant decrease in total antioxidant status 24 h after deflation were recorded in group 2. In addition, a significant increase was observed in ALT, AST and LDH levels. These changes were attenuated by low-pressure pneumoperitoneum, whereas pentoxifylline pretreatment appeared to attenuate only transaminase levels.


CONCLUSIONS
Low-pressure pneumoperitoneum could attenuate ischemia/reperfusion injury induced by CO(2 )pneumoperitoneum in a rabbit model whereas pentoxifylline pretreatment appeared to attenuate only transaminase levels. Pentoxifylline did not prevent the development of oxidative stress.
Department of Anesthesiology, Resuscitation and Intensive Care, University of Zagreb, Zagreb, Croatia. tihomir.adam@zg.htnet.hr
V
V
Nesek-Adam
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D
Vnuk
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Z
Rasić
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V
Rumenjak
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J
Kos
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Krstonijević
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002245:Carbon Dioxide; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010431:Pentoxifylline / Q000494:pharmacology*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011312:Pressure; D011817:Rabbits; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control
D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D002245:Carbon Dioxide; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D010431:Pentoxifylline