Development and application of quantitative-PCR tools for subgroups of the Roseobacter clade.
Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment.
Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA. abuchan@utk.edu
Alison
A
Buchan
Mary
M
Hadden
Marcelino T
MT
Suzuki
D001431:Bacteriological Techniques / Q000379:methods*; D052160:Benzothiazoles; D044822:Biodiversity; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003959:Diamines; D004783:Environmental Microbiology; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D009930:Organic Chemicals; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D041904:Roseobacter / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013194:Staining and Labeling / Q000379:methods
D052160:Benzothiazoles; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I