Identification of group I-III avian adenovirus by PCR coupled with direct sequencing of the hexon gene.
Polymerase chain reaction (PCR) coupled with direct sequencing of the product of the hexon gene was applied to avian adenoviruses (formerly group I-III). The expected sizes of DNA fragments were successfully amplified by PCR from all of the group I-III avian adenoviruses with our designed primers. The resulting PCR product contained diagnostically relevant hexon sequences that could be used to identify the group and type of avian adenovirus.
National Institute of Animal Health, Kannondai Tsukuba, Ibaraki, Japan. masema@affrc.go.jp
Masaji
M
Mase
Hiromichi
H
Mitake
Toshikazu
T
Inoue
Tadao
T
Imada
D000165:Acridine Orange; D000259:Aviadenovirus / Q000145:classification* / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary*
D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus; D000165:Acridine Orange