Identification of novel stress-regulated microRNAs from Oryza sativa L.
MicroRNAs (miRNAs) are a type of small non-coding RNA found in eukaryotes. They play a key role in gene expression by down-regulating gene expression and are involved in the environment stress response in plants. Although a large number of miRNAs have been identified from Arabidopsis, few studies have focused on Oryza sativa miRNAs, especially on stress-related miRNAs. Five cDNA libraries of small RNAs from rice seedlings treated with cold, dehydration, salinity, and abscisic acid (ABA), as well as wild-type seedlings, were constructed. Seven rice novel miRNAs were identified by Northern analysis, and their expression patterns under different stress treatments were determined. Results showed that the expression of several novel miRNAs was regulated by one or more stress treatments. Our identification of novel stress-related miRNAs in rice suggests that these miRNAs might be involved in rice stress response pathways.
Jian
Xinyu
X
National Centre for Plant Gene Research, Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, P.O. Box 2707, South 1-3, Zhongguancun, Beijing 100080, P.R. China.
Zhang
Lin
L
Li
Guanglin
G
Zhang
Liang
L
Wang
Xiujie
X
Cao
Xiaofeng
X
Fang
Xiaohua
X
Chen
Fan
F
D000040:Abscisic Acid / Q000737:chemistry; D000222:Adaptation, Physiological / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D006863:Hydrogen-Ion Concentration; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D054712:Salinity; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014867:Water
D035683:MicroRNAs; D018749:RNA, Plant; D014867:Water; D000040:Abscisic Acid