Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection.
Affymetrix GeneChip arrays are a powerful tool for transcriptome profiling and have been applied to a wide range of species. A genomic DNA (gDNA)-based probe selection method has been developed which broadens the range of species to which GeneChips may be successfully applied. This study demonstrated that gDNA-based probe selection on the Affymetrix U133+2 GeneChip array can be used to study the equine transcriptome which, to date, has received only limited attention. More than 29,000 transcripts can be detected in equine brain and liver and in primary cultures of equine articular chondrocytes. Gene ontology analysis of differentially expressed genes revealed the presence of expected categories within each tissue. The level of gene expression could also be correlated with the phenotypes and specialised functions of each tissue. The results demonstrated that probe selection on a human chip can be successfully used to study the equine transcriptome.
Nottingham Arabidopsis Stock Centre, Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough, UK.
Neil S
NS
Graham
Abigail L
AL
Clutterbuck
Nicholas
N
James
Richard G
RG
Lea
Ali
A
Mobasheri
Martin R
MR
Broadley
Sean T
ST
May
D000818:Animals; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D015870:Gene Expression; D016678:Genome; D006736:Horses / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D012641:Selection, Genetic; D013045:Species Specificity; D014158:Transcription, Genetic
D015342:DNA Probes; D004247:DNA