Cytokine-related genes and oxidation-related genes detected in preeclamptic placentas.
OBJECTIVE
To investigate cytokine- and oxidation-related genes for preeclampsia using DNA microarray analysis.


METHODS
Placentas were collected from 13 normal pregnancies and 13 patients with preeclampsia. Gene expression was studied using DNA microarray. Among significantly expressed genes, we focused on genes associated with cytokines and oxidation, and the results were confirmed using quantitative real time-polymerase chain reaction (QRT-PCR).


RESULTS
415 genes out of 30,940 genes were altered by > or =2-fold in the microarray analysis. 121 up-regulated genes and 294 down-regulated genes were found to be in preeclamptic placenta. Six cytokine-related genes and 5 oxidation-related genes were found from among the 121 up-regulated genes. The cytokine-related genes studied included oncostatin M (OSM), fms-related tyrosine kinase (FLT1) and vascular endothelial growth factor A (VEGFA), and the oxidation-related genes studied included spermine oxidase (SMOX), l cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), acetate dehydrogenase A (LDHA). These six genes were also significantly higher in placentas from patients with preeclampsia than in those from women with normal pregnancies. The placental tissue of patients with preeclampsia showed significantly higher mRNA expression of these six genes than the normal group, using QRT-PCR.


CONCLUSIONS
DNA microarray analysis is one of the great methods for simultaneously detecting the functionally associated genes of preeclampsia. The cytokine-related genes such as OSM, FLT1 and VEGFA, and the oxidation-related genes such as LDHA, CYP26A1 and SMOX might prove to be the starting point in the elucidation of the pathogenesis of preeclampsia.
Lee
Gui Se Ra
GS
Department of Obstetrics and Gynecology, Catholic University of Korea, Soechoe-dong, Banpo-gu, Seoul, Republic of Korea.
Joe
Yoon Seong
YS
Kim
Sa Jin
SJ
Shin
Jong Chul
JC
D000328:Adult; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000098:blood supply / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger; D015854:Up-Regulation
D016207:Cytokines; D012333:RNA, Messenger