Generation of neutralizing monoclonal antibodies against Enterovirus 71 using synthetic peptides.
Enterovirus 71 (EV71) has led to recent outbreaks of hand, foot and mouth disease (HFMD) in China, resulting in high mortality. In this study, several monoclonal antibodies were generated by immunizing mice with two synthetic peptides, SP55 and SP70, containing amino acids 163-177 and 208-222 of VP1. The specificities of the anti-EV71 peptide monoclonal antibodies were confirmed by Western blot analysis and immunocytochemistry against EV71 virus. Most importantly, we have identified a monoclonal antibody, clone 22A12, which shows strong neutralizing activity against EV71 in an in vitro neutralization assay. Because there is no vaccine available and treatment is very limited, mouse anti-EV71 monoclonal antibody, clone 22A12, could be a promising candidate to be humanized and used for treatment of EV71 infection.
Li
Xiuling
X
Beijing Vigoo Biologicals Co., Ltd, 4 South San Jian Fan, Chaoyang District, Beijing 100024, China.
Mao
Chunming
C
Ma
Shujun
S
Wang
Xiaoqing
X
Sun
Zhe
Z
Yi
Yao
Y
Guo
Minzhuo
M
Shen
Xinliang
X
Sun
Le
L
Bi
Shengli
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000627:therapeutic use; D057134:Antibodies, Neutralizing / Q000096:biosynthesis* / Q000627:therapeutic use; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000627:therapeutic use; D003384:Coxsackievirus Infections / Q000276:immunology / Q000628:therapy / Q000821:virology; D029821:Enterovirus A, Human / Q000276:immunology*; D006232:Hand, Foot and Mouth Disease / Q000276:immunology / Q000628:therapy / Q000821:virology; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D010455:Peptides / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology* / Q000627:therapeutic use
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D010455:Peptides; D014765:Viral Vaccines