Effect of noise on DNA sequencing via transverse electronic transport.
Previous theoretical studies have shown that measuring the transverse current across DNA strands while they translocate through a nanopore or channel may provide a statistically distinguishable signature of the DNA bases, and may thus allow for rapid DNA sequencing. However, fluctuations of the environment, such as ionic and DNA motion, introduce important scattering processes that may affect the viability of this approach to sequencing. To understand this issue, we have analyzed a simple model that captures the role of this complex environment in electronic dephasing and its ability to remove charge carriers from current-carrying states. We find that these effects do not strongly influence the current distributions due to the off-resonant nature of tunneling through the nucleotides--a result we expect to be a common feature of transport in molecular junctions. In particular, only large scattering strengths, as compared to the energetic gap between the molecular states and the Fermi level, significantly alter the form of the current distributions. Since this gap itself is quite large, the current distributions remain protected from this type of noise, further supporting the possibility of using transverse electronic transport measurements for DNA sequencing.
Krems
Matt
M
Department of Physics, University of California, San Diego, La Jolla, California, USA.
Zwolak
Michael
M
Pershin
Yuriy V
YV
Di Ventra
Massimiliano
M
D000227:Adenine Nucleotides / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004553:Electric Conductivity; D004579:Electron Transport; D056004:Molecular Dynamics Simulation; D009690:Nucleic Acid Conformation; D011336:Probability; D017422:Sequence Analysis, DNA / Q000379:methods*; D013997:Time Factors
D000227:Adenine Nucleotides; D004247:DNA