The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths.
DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25ng) compared to the absence of RNA (5ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications.
Shaw
Kirsty J
KJ
Department of Chemistry, University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom.
Thain
Lauren
L
Docker
Peter T
PT
Dyer
Charlotte E
CE
Greenman
John
J
Greenway
Gillian M
GM
Haswell
Stephen J
SJ
D004247:DNA / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012313:RNA / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction
D012313:RNA; D012822:Silicon Dioxide; D004247:DNA