Expression profiling of the AT2R mRNA in affected tissue from children with CAKUT.
OBJECTIVE
Congenital anomalies of the kidney and urinary tract (CAKUT) are common causes of chronic renal failure in children. The angiotensin II receptor type 2 (AT2R) is one of proposed candidate genes for CAKUT, but the expression was never explored in humans. The aim was to establish the AT2R gene expression in human CAKUT concerning -1332A/G polymorphism, which might affect alternative splicing.


METHODS
Forty-eight patients with CAKUT constitute the basis of this study. Genotyping for -1332A/G, RT-PCR for AT2R gene expression and confirmation sequencing were performed.


RESULTS
The expression of Ex 1/2/3 and Ex 1/3 transcript splice variants of the AT2R mRNA were detected in human CAKUT tissue. The pattern was observed independently of A to G transition.


CONCLUSIONS
The expression of AT2R mRNA in human CAKUT was established for the first time and was not affected by -1332A/G polymorphism in children with CAKUT.
Vinca Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, 11001 Belgrade, Serbia. alexas@vinca.rs
Aleksandra
A
Stanković
Maja
M
Zivković
Mirjana
M
Kostić
Jasmina
J
Atanacković
Zoran
Z
Krstić
Dragan
D
Alavantić
D017398:Alternative Splicing; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D007668:Kidney / Q000002:abnormalities*; D007676:Kidney Failure, Chronic / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D014551:Urinary Tract / Q000002:abnormalities*
D012333:RNA, Messenger; D044139:Receptor, Angiotensin, Type 2