[Tissue-engineering bone with ADSCs and coral scaffold for repairing of cranial bone defect in canine].
OBJECTIVE
To investigate the application of tissue-engineering bone with ADSCs (adipose-derived stem cells) and coral scaffold for repairing of cranial bone defect in canine.


METHODS
Autologous ADSCs isolated from canine subcutaneous fat were expanded, osteogenically induced, and seeded on coral scaffolds. Bilateral full-thickness defects (20 mm x 20 mm) of parietal bone were created (n = 7). The defects were either repaired with ADSC-coral constructs (experimental group) or with coral alone (control group). Radiological, gross, biomechanical and histological observations were done to evaluate the bone regeneration.


RESULTS
Three-dimensional CT scan showed that new bones were formed in the experimental group at 12 weeks after implantation, while coral scaffolds were partially degraded in the control group. By radiographic analysis at 24 weeks post-transplantation, it showed that an average repair percentage of each defect was (84.19 +/- 6.45)% in experimental group, and (25.04 +/- 18.82)% in control group (P < 0.01). The maximum compression loading was (73.45 +/- 17.26) N in experimental group, and (104.27 +/- 22.71) N in control group (P <0.01). Histological examination revealed that the defect was repaired by typical bone tissue in experimental group, while only minimal bone formation with fibrous connection in the control group.


CONCLUSIONS
The tissue-engineering bone with autologous osteogenic ADSCs and scaffold could successfully repair the cranial defects in canine models.
Department of Plastic and Reconstructive Surgery, Zhejiang Traditional Chinese Medicine Hospital, Hangzhou 310006, China.
Bo
B
Liu
Lei
L
Cui
Guang-Peng
GP
Liu
Yi-Lin
YL
Cao
Jin-Tu
JT
Zhu
Yi
Y
Cao
D017667:Adipocytes / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D037421:Anthozoa; D001861:Bone Regeneration; D018786:Bone Substitutes; D001842:Bone and Bones; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D008297:Male; D012886:Skull / Q000601:surgery*; D033581:Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous
D018786:Bone Substitutes