Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates.
BACKGROUND
In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics.


OBJECTIVE
To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method.


METHODS
One hundred and forty-seven clinical isolates of M. tuberculosis were studied. For the TLA method, a quadrant Petri plate containing 7H11 agar with RMP, OFX and KM was used. Results were compared to the Bactec MGIT960 for RMP and the proportion method for OFX and KM.


RESULTS
The sensitivity and specificity for RMP and OFX were 100% and for KM they were 100% and 98.7%, respectively. The use of a TLA quadrant plate enables the rapid detection of resistance to the three anti-tuberculosis drugs RMP, OFX and KM in a median of 10 days.


CONCLUSIONS
TLA was an accurate method for the detection of resistance in the three drugs studied. This faster method is simple to perform, providing an alternative method when more sophisticated techniques are not available in low-resource settings.
Martin
A
A
Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium. amartin@itg.be
Paasch
F
F
Von Groll
A
A
Fissette
K
K
Almeida
P
P
Varaine
F
F
Portaels
F
F
Palomino
J-C
JC
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods; D024881:Drug Resistance, Bacterial; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D015242:Ofloxacin / Q000494:pharmacology; D012293:Rifampin; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D000995:Antitubercular Agents; D007612:Kanamycin; D000362:Agar; D015242:Ofloxacin; D012293:Rifampin