[Serum proteomics in patients with RAEB myelodysplastic syndromes].
OBJECTIVE
To screen the molecular markers for refractory anemia with excess blasts in transformation (RAEB) in myelodysplastic syndromes (MDS) by serum proteome profiling.


METHODS
The serum protein were isolated from patients with RAEB, acute myeloid leukemia or normal subjects by 2-dimensional electrophoresis (2-DE), and the electrophoresis gels were obtained to identify the differentially reacting protein spots. The replica gels of the differentially reacting proteins were analyzed to locate the matching protein spots, which were identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of-flight mass spectrometry (MALDI-TOF-MS) and database searching.


RESULTS
Seven differentially expressed proteins in RAEB were found by 2-DE. Of the 7 proteins, 4 were identified by MALDI-TOF-MS to have significantly differential expression in RAEB, including dipeptidyl peptidase (DPP/CD26), polymerase (DNA directed) kappa, PRO2044 and an albumin-like protein.


CONCLUSIONS
2-DE-based serum proteome profiling helps identify serum proteomic biomarkers related to MDS. DDP/CD26 has increased expression in the serum in RAEB subtype MDS, suggesting its possible role in advanced MDS.
Zhong
Li-ye
LY
Department of Hematology, Guangdong General Hospital/Guangdong Academy of Medical Sciences, Guangzhou, China. zhongliye99@163.com
Liu
Tian-hao
TH
Li
Yang-qiu
YQ
Geng
Su-xia
SX
Lu
Ze-sheng
ZS
Weng
Jian-yu
JY
Wu
Sui-jing
SJ
Luo
Cheng-wei
CW
Du
Xin
X
D000754:Anemia, Refractory, with Excess of Blasts / Q000097:blood* / Q000235:genetics; D001853:Bone Marrow / Q000473:pathology; D004259:DNA-Directed DNA Polymerase / Q000097:blood*; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood* / Q000145:classification / Q000235:genetics; D040901:Proteomics; D000099270:Y-Family DNA Polymerases
D004259:DNA-Directed DNA Polymerase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C404302:POLK protein, human; D000099270:Y-Family DNA Polymerases