Synthesis of poly(o-anisidine)/H(2)SO(4) film for the development of glucose biosensor.
The poly(o-anisidine)-sulfuric acid-glucose oxidase (POA-H(2)SO(4)-GOx) electrode has been investigated in the present work. Platinum electrode was used for the synthesis of poly (o-anisidine)-sulfuric acid (POA-H(2)SO(4)) film using galvanostatic method with 0.2 M o-anisidine, 1.0 M H(2)SO(4) solution, 1.0 pH and 2 mA/cm(2) applied current density. The synthesized film was characterized using electrochemical technique, conductivity measurement, UV-visible spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy. GO(X) was immobilized on synthesized POA-H(2)SO(4) film by cross-linking via glutaraldehyde in phosphate and acetate buffer. The Michaelis-Menten constant (K(m')) was determined for the immobilized enzyme. The glucose oxidase electrode shows the maximum current response at pH 5.5 and potential 0.6 V. The sensitivity of POA-H(2)SO(4)-GO(X) electrode in phosphate and acetate buffer has been recorded. The results of this study reveal that the phosphate buffer gives fast response as compared to acetate buffer in amperometric measurements.
Savale
P A
PA
Optoelectronics and Sensor Research Laboratory, Department of Physics, Dr Babasaheb Ambedkar Marathwada University, Aurangabad 431004, India. pa_savale@yahoo.co.in
Shirsat
M D
MD
D000814:Aniline Compounds / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D008567:Membranes, Artificial; D013464:Sulfuric Acids / Q000737:chemistry*
D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D013464:Sulfuric Acids; D005949:Glucose Oxidase; D005947:Glucose; C033158:sulfuric acid