Towards profiling the gene expression of tyrosinase-induced melanogenesis in HEK293 cells: a functional DNA chip microarray and interactomics studies.
The overexpression of a single tyrosinase gene can induce conspicuous pigmentation in nonpigmented cells. We hypothesized that some unknown tyrosinase-associated genes are simultaneously regulated by melanin synthesis. To improve understanding of melanogenesis and tyrosinase-associated functions, we attempted to profile the genes that are altered during melanin production in HEK293 cells by using a functional DNA chip microarray. The candidate genes were obtained based on significance analysis of microarray (SAM) and further computational prediction via protein-protein interaction (PPI) mapping suggested that newly detected hub genes were involved in melanogenesis. PPI mapping using bioinformatic tools revealed 8 genes that formed an interaction hub. The yeast two-hybridization results suggested some candidate genes could interact with tyrosinase. The present study provides information to further understand the complex factors associated with tyrosinase-induced melanogenesis and apoptosis. The approach of combining expression data analysis and predicted protein interaction partners can help identify genes involved in pigmentation.
Cho
Ick-Hyun
IH
Department of Biotechnology, Yonsei University, Seoul, Korea.
Lü
Zhi-Rong
ZR
Yu
Jae-Ran
JR
Park
Yong-Doo
YD
Yang
Jun-Mo
JM
Hahn
Myong-Joon
MJ
Zou
Fei
F
D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008543:Melanins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D014442:Monophenol Monooxygenase / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D012880:Skin Pigmentation / Q000235:genetics
D008543:Melanins; D012333:RNA, Messenger; D014442:Monophenol Monooxygenase