[Improve on serological diagnosis method of nasopharyngeal carcinoma].
OBJECTIVE
To improve the existing serological early diagnosis method of nasopharyngeal carcinoma by improve the detection sensitivity.


METHODS
The samples of 294 serum specimen from the prevention and treatment of nasopharyngeal cancer model base, involving 106 serum specimen from the patients suffering from nasopharyngeal cancer and 188 from the healthy testers. IgA/VCA antibody and IgA/EA antibody of the serums are tested by Streptavidin-biotin-antibody immunoenzymatic test and normal traditional enzyme methods, SPSS statistical software is used to analyse the test results with chi2 test and t test.


RESULTS
Referring to 106 patients, the sera antibody positive rate tested by Streptavidin-biotin-antibody immunoenzymatic test method is obviously higher than that tested by traditional method; and the t test result of the GMT has significant difference in the two method.


CONCLUSIONS
The modified method can improve the sensitivity of serology testing, ensure the specificity of test results, at the same time, improve the detection rate of nasopharyngeal carcinoma, so it can be applied to the early screen work of nasopharyngeal carcinoma.
Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Shao-Bing
SB
Zhan
Jian-Ming
JM
Zhong
Zhi-Ping
ZP
Mai
Shu-Qing
SQ
Ye
Ling
L
Zhou
Yi
Y
Zeng
Jian
J
Liao
D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*
D000914:Antibodies, Viral; D007070:Immunoglobulin A