Targeted transduction via CD4 by a lentiviral vector uses a clathrin-mediated entry pathway.
We recently developed a novel targeting Sindbis virus envelope pseudotyped lentiviral vector, 2.2ZZ, which acquires specific transduction capacity by antibody conjugation and binding with specific antigens on the surface of targeted cells. Here we characterize the virological properties of this vector by examining its targeting to CD4 antigen. Our results show that entry is dependent on CD4 cell surface density and occurs via the clathrin-mediated endocytic pathway. These findings provide insight into the mechanism of infection by a new viral vector with combined properties of Sindbis virus and lentiviruses and infectivity conferred by monoclonal antibody-ligand interactions.
Liang
Min
M
Department of Microbiology, Immunology and Molecular Genetics, and Medicine, UCLA AIDS Institute, 615 Charles E. Young Dr. East, BSRB 173, Los Angeles, CA 90095, USA.
Morizono
Kouki
K
Pariente
Nonia
N
Kamata
Masakazu
M
Lee
Benhur
B
Chen
Irvin S Y
IS
D015704:CD4 Antigens / Q000502:physiology*; D002966:Clathrin / Q000502:physiology*; D004705:Endocytosis; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D014161:Transduction, Genetic
D015704:CD4 Antigens; D002966:Clathrin