Surface plasmon resonance biosensor based on water-soluble ZnO-Au nanocomposites.
A wavelength modulation surface plasmon resonance biosensor based on ZnO-Au nanocomposites for the detection of human IgM was developed. Self-assembly technique has the advantages of flexibility, simplicity and the precise control of film component and was applied to the building of the sensor. The ZnO-Au nanocomposites are in a dumbbell-like shape and can be immobilized on the Au film through 1,6-hexanedithiol by covalent attachment. Meanwhile the activated ZnO nanocrystals can be used to connect protein. The biosensor based on ZnO-Au nanocomposites was used to detect human IgM. Some experimental conditions were examined and optimized. In the selected conditions, the modified biosensor exhibits a satisfactory response for human IgM in the concentration range of 0.30-20.00 microg mL(-1). However, the biosensor without ZnO-Au nanocomposites shows a response for human IgM in the concentration range of 1.25-20.00 microg mL(-1). Compared with the biosensor based on Au film, when the biosensor based on the ZnO-Au nanocomposites was applied, the sensitivity for determination of human IgM is significantly enhanced.
Wang
Liying
L
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Wang
Jian
J
Zhang
Songling
S
Sun
Ying
Y
Zhu
Xiaonan
X
Cao
Yanbo
Y
Wang
Xinghua
X
Zhang
Hanqi
H
Song
Daqian
D
D055910:Antibodies, Immobilized / Q000276:immunology / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D014867:Water / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
D055910:Antibodies, Immobilized; D007075:Immunoglobulin M; D013438:Sulfhydryl Compounds; D014867:Water; D006046:Gold; C501433:1,6-hexanedithiol; D015034:Zinc Oxide