Protein engineering of a fibroblast growth factor-1 fusion protein with cell adhesive activity.
Fibroblast growth factor-1 (FGF1) is one of the most potent angiogenic growth factors, and also plays an important role in regulating cellular functions including cell proliferation, motility, differentiation, survival, and tissue regeneration processes. Here we described a novel fusion protein that was designed by combining the cell adhesion sequence from fibronectin with FGF1. The F1-Fn fusion protein functions as a minimized protein that directs integrin-dependent cell adhesion and stimulates cellular responses including cell proliferation and differentiation. Moreover, our results indicate that Fn-mediated signaling synergizes with signals from FGF1 in promoting cellular adhesion, proliferation, and differentiation in MG63 cells.
Jeon
Eunyi
E
Department of Biochemistry, Inha University School of Medicine, Incheon, Korea. juhjang@inha.ac.kr
Kim
Hae-Won
HW
Jang
Jun-Hyeog
JH
D000217:Acyltransferases; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D016220:Fibroblast Growth Factor 1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology; D015202:Protein Engineering / Q000379:methods; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; D016220:Fibroblast Growth Factor 1; D000217:Acyltransferases; C491188:PORCN protein, human