Toxicity and carcinogenicity studies of methylene blue trihydrate in F344N rats and B6C3F1 mice.
Methylene blue trihydrate has a variety of biomedical and biologically therapeutic applications. Groups of 50 male and 50 female rats and mice were administered methylene blue trihydrate in 0.5% aqueous methylcellulose solution by gavage at doses of 0, 5, 25, or 50mg/kg bw/day (rats) or 0, 2.5, 12.5, and 25mg/kg bw/day (mice), 5 days per week for 2 years. In rats survival of all dosed groups was similar to that of the vehicle controls, whereas mice exhibited a dose-dependent increase in survival. Rats receiving 25 and 50mg/kg bw/day and mice receiving 25mg/kg bw/day developed mild anemia. The incidences of pancreatic islet cell adenoma and adenoma or carcinoma (combined) were increased in all dosed groups of male rats, but increases were statistically significant in 25mg/kg bw/day males only and the dose-response was non-linear. There was a corresponding increase in the incidence of pancreatic islet cell hyperplasia but statistically significant only in the 50mg/kg bw/day male rats. There were no significant increases in neoplastic transformation observed in the mice; however, positive trends were noted for adenoma or carcinoma (combined) of the small intestine and malignant lymphoma.
Auerbach
Scott S
SS
National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, United States. auerbachs@niehs.nih.gov
Bristol
Douglas W
DW
Peckham
John C
JC
Travlos
Gregory S
GS
Hébert
Charles D
CD
Chhabra
Rajendra S
RS
D000818:Animals; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D053208:Kaplan-Meier Estimate; D008168:Lung / Q000473:pathology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology; D008751:Methylene Blue / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains; D009369:Neoplasms / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D012727:Sex Characteristics; D013045:Species Specificity; D016019:Survival Analysis
D002273:Carcinogens; D008751:Methylene Blue