MCP-1 gene A-2518G polymorphism and carotid artery atherosclerosis in patients with type 2 diabetes.
OBJECTIVE
Cardiovascular diseases are the major cause of mortality in patients with diabetes mellitus. Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine and plays an important role in cardiovascular diseases. The objective of this study was to evaluate the relation between the genotypes of the MCP-1 A-2518G polymorphism and the development of carotid atherosclerosis in patients with type 2 diabetes.


METHODS
The subjects were 303 unrelated patients who were diagnosed with type 2 diabetes mellitus. To evaluate macroangiopathy, we measured carotid artery intima-media thickness (IMT) by ultrasonography. The MCP-1 A-2518G polymorphism was determined by TaqMan PCR method.


RESULTS
IMT in patients with the MCP-1 -2518 AG or GG genotype was significantly greater than the AA-genotype (P=0.007). Simple regression analysis showed that age, systolic blood pressure, LDL-cholesterol, the MCP-1 -2518 AG+GG polymorphism, and HbA1c level were correlated with IMT (P<0.0001, <0.0001, 0.006, 0.007, 0.025, respectively). In multiple regression analysis, the MCP-1 -2518 AG+GG polymorphism was the third strongest independent determinant of IMT in patients with type 2 diabetes (P=0.021), subsequent to age and systolic blood pressure.


CONCLUSIONS
Assessment of the MCP-1 A-2518G polymorphism would be useful in identifying the risk of developing carotid atherosclerosis in patients with type 2 diabetes.
Division of Metabolism, Department of Internal Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan. sachicom@mvj.biglobe.ne.jp
Sachiko
S
Yuasa
Taro
T
Maruyama
Yukihiro
Y
Yamamoto
Hiroshi
H
Hirose
Toshihide
T
Kawai
Seiko
S
Matsunaga-Irie
Hiroshi
H
Itoh
D000225:Adenine; D000368:Aged; D044466:Asian People / Q000235:genetics; D002340:Carotid Artery Diseases / Q000150:complications / Q000000981:diagnostic imaging / Q000235:genetics*; D018932:Chemokine CCL2 / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000235:genetics; D003925:Diabetic Angiopathies / Q000000981:diagnostic imaging / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012044:Regression Analysis; D013941:Thymine; D014463:Ultrasonography
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D000225:Adenine; D013941:Thymine