[The infection of dendritic cells by recombinant adenoviral vector carrying HBsAg-HSP70 chimeric gene and its biological characteristics observations].
OBJECTIVE
To test the infeciton efficiency of recombinant adenoviral vector carrying HBsAg-HSP70 chimeric gene and to abserve its biological characteristics.


METHODS
Peripheral blood mononuclear cells (PBMC) were separated from healthy blood donor and they were infected by Ad-HSP70-HBsAg on the first day of isolation. DCs were induced in medium with cytokines IL-4, GM-CSF and TNF-alpha in vitro. The biological characteristics of DC induced were analyzed by inverted fluorecent microscope, RT-PCR, flow cytometer (FACS), and mixed lymphocyte reaction (MLR).


RESULTS
The traced gene-GFP were abserved in DCs by inverted fluorecent microscope and HSP70-HBsAg gene mRNA expression was detected by RT-PCR after the Ad-HSP70-HBsAg infection. FACS analysis shown that the expression of CD1a, CD80, CD86 and HLA-DR on surfece of two groups of DCs were similar. MLR showed that there are not a statitic difference of stimulated index (SI) between two groups.


CONCLUSIONS
Results indicated that Ad-HSP70-HBsAg can effectively infected DCs without affecting its biological characteristics.
The Eighth Hospital of Guangzhou City, China.
Chun-Liang
CL
Lei
Yang-Ling
YL
Ou
Zhan
Z
Yang
Xiao-Ping
XP
Tang
D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology*; D005822:Genetic Vectors / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D006514:Hepatitis B Surface Antigens; D011993:Recombinant Fusion Proteins