Quantification of the sulfated cholecystokinin CCK-8 in hamster plasma using immunoprecipitation liquid chromatography-mass spectrometry/mass spectrometry.
Cholecystokinin (CCK) and the different molecular forms of CCK are well established as biomarkers for satiety but accurate analysis has been limited by the multiple naturally occurring forms and extensive similarities to gastrin. Changes in levels of one form, CCK-8, a naturally occurring eight amino acid peptide of CCK, have been correlated with satiety responses. Endogenous CCK-8 has not been well characterized in Syrian Golden hamsters, an important model in the study of fat uptake and digestion. We have cloned and sequenced hamster CCK and identified and characterized endogenous CCK-8 from hamster plasma. Hamster CCK-8 is composed of eight amino acid residues which are highly conserved among other species. Following accurate identification and characterization of hamster CCK-8, we have developed a highly specific and sensitive immunoprecipitation based LC-MS/MS assay for its quantification. The present assay enables determination of active CCK-8 over a concentration range from 0.05 to 2.5 ng/mL in hamster plasma samples. This range covers both the basal and postprandial levels of CCK-8. Method performance validation samples were examined at three concentrations replicated over the course of 4 days. The assay accuracy (percent relative error, % RE) average was 11.3% with a precision (percent coefficient of variation, % CV) of 15.5% over all samples in this 4 day period. Additionally, the method was used to determine increases of endogenous plasma CCK-8 in hamsters challenged with a high-fat meal.
The Dow Chemical Company, Analytical Sciences, BioAnalytical, 1897 Building, Midland, Michigan 48667, USA. sayoung@dow.com
Scott A
SA
Young
Samir
S
Julka
Glenn
G
Bartley
Jeffrey R
JR
Gilbert
Brian M
BM
Wendelburg
Shao-Ching
SC
Hung
W H Kerr
WH
Anderson
Wallace H
WH
Yokoyama
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002766:Cholecystokinin / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006224:Cricetinae; D004041:Dietary Fats; D047468:Immunoprecipitation / Q000379:methods*; D008647:Mesocricetus; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000097:blood*; D016415:Sequence Alignment; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
D004041:Dietary Fats; D010446:Peptide Fragments; C410885:cholecystokinin 8; C005662:tyrosine O-sulfate; D014443:Tyrosine; D002766:Cholecystokinin