Modulating and targeting meiotic double-strand breaks in Saccharomyces cerevisiae.
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10-100-fold from site to site. Herein are reviewed the methods used in budding yeast to modulate locally and globally the native DSB frequencies, including a powerful method to target Spo11-dependent meiotic DSB in novel chromosomal regions. These methods serve to investigate the control and the mechanism of recombination initiation and modify the natural distribution of meiotic recombination.
Institut Curie, Centre de Recherche, UMR7147-CNRS, Université Pierre et Marie Curie, Paris, France.
Alain
A
Nicolas
D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics / Q000502:physiology*; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D004706:Endodeoxyribonucleases; D018390:Gene Targeting / Q000379:methods; D008540:Meiosis / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D030781:Organisms, Genetically Modified; D011995:Recombination, Genetic / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D000099257:Meiotic Recombination Protein SPO11
D004250:DNA Topoisomerases, Type II; D004706:Endodeoxyribonucleases; D029701:Saccharomyces cerevisiae Proteins; C518938:Spo11 protein, S cerevisiae; D000099257:Meiotic Recombination Protein SPO11