Profiling differential expression of cellulases and metabolite footprints in Aspergillus terreus.
This study reports differential expression of endoglucanase (EG) and beta-glucosidase (betaG) isoforms of Aspergillus terreus. Expression of multiple isoforms was observed, in presence of different carbon sources and culture conditions, by activity staining of poly acrylamide gel electrophoresis gels. Maximal expression of four EG isoforms was observed in presence of rice straw (28 U/g DW substrate) and corn cobs (1.147 U/ml) under solid substrate and shake flask culture, respectively. Furthermore, the sequential induction of EG isoforms was found to be associated with the presence of distinct metabolites (monosaccharides/oligosaccharides) i.e., xylose (X), G(1), G(3) and G(4) as well as putative positional isomers (G(1)/G(2), G(2)/G(3)) in the culture extracts sampled at different time intervals, indicating specific role of these metabolites in the sequential expression of multiple EGs. Addition of fructose and cellobiose to corn cobs containing medium during shake flask culture resulted in up-regulation of EG activity, whereas addition of mannitol, ethanol and glycerol selectively repressed the expression of three EG isoforms (Ia, Ic and Id). The observed regulation profile of betaG isoforms was distinct when compared to EG isoforms, and addition of glucose, fructose, sucrose, cellobiose, mannitol and glycerol resulted in down-regulation of one or more of the four betaG isoforms.
Nazir
Asiya
A
Department of Microbiology, Guru Nanak Dev University, Amritsar 143 005, India.
Soni
Rohit
R
Saini
H S
HS
Kaur
Amarjeet
A
Chadha
B S
BS
D001230:Aspergillus / Q000737:chemistry* / Q000378:metabolism; D002480:Cellulase / Q000032:analysis* / Q000378:metabolism; D015536:Down-Regulation; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D013379:Substrate Specificity; D015854:Up-Regulation; D001617:beta-Glucosidase / Q000032:analysis* / Q000378:metabolism
D007527:Isoenzymes; D001617:beta-Glucosidase; D002480:Cellulase