Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria.
In this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.
School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.
Christopher H
CH
Miller
Shahista
S
Nisa
Sandi
S
Dempsey
Cameron
C
Jack
Ronan
R
O'Toole
D000995:Antitubercular Agents / Q000494:pharmacology*; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development*; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000254:growth & development; D016566:Organoselenium Compounds / Q000494:pharmacology; D017279:Selenocysteine / Q000031:analogs & derivatives; D054852:Small Molecule Libraries
D000995:Antitubercular Agents; D016566:Organoselenium Compounds; D054852:Small Molecule Libraries; D017279:Selenocysteine; D003545:Cysteine; C002979:selenomethylselenocysteine