[Effects of hydrogen sulfide on the K ATP current in isolated rat ventricular myocytes].
OBJECTIVE
To investigate the effects of endogenous and exogenous hydrogen sulfide (H(2)S) on the K(ATP) current in isolated rat ventricular myocytes.


METHODS
Ventricular myocytes were isolated from rat heart by modified Langendorff perfusion with collagenase. K(ATP) current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique.


RESULTS
The density of K(ATP) current was significantly reduced by 200 micromol/L DL-propargylglycine (PPG, an irreversible inhibitor of the H(2)S) [(5.3258 +/- 0.7556) pA/pF vs. (3.7856 +/- 0.4312) pA/pF, P < 0.01] in a time-dependent way. The density of K(ATP) current could be significantly increased by NaHS (a H(2)S donor, 9.375, 18.75, 37.5, 75, 150 micromol/L) in a concentration-dependent manner [(6.6310 +/- 0.6092) pA/pF vs. (9.0949 +/- 1.0259) pA/pF at 150 micromol/L, P < 0.01].


CONCLUSIONS
Both endogenous and exogenous H(2)S could open K(ATP) channels and enhance the K(ATP) current in rat ventricular myocytes.
Department of Cardiology, Chaoyang Hospital, Capital Medical University, Beijing, China.
Yan-bing
YB
Li
Guang-zhen
GZ
Zhong
Xin-chun
XC
Yang
Xiu-lan
XL
Liu
D000818:Animals; D002478:Cells, Cultured; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D054086:KATP Channels / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar
D054086:KATP Channels; D006862:Hydrogen Sulfide