A fluorescence lifetime imaging scanning confocal endomicroscope.
We describe a fluorescence lifetime imaging endomicroscope employing a fibre bundle probe and time correlated single photon counting. Preliminary images of stained pollen grains, eGFP-labelled cells exhibiting Förster resonant energy transfer and tissue autofluorescence are presented.
Kennedy
Gordon T
GT
Manning
Hugh B
HB
Elson
Daniel S
DS
Neil
Mark A A
MA
Stamp
Gordon W
GW
Viellerobe
Bertrand
B
Lacombe
François
F
Dunsby
Chris
C
French
Paul M W
PM
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D019723:Endoscopes; D004724:Endoscopy / Q000379:methods*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D017785:Photons; D011058:Pollen; D051381:Rats; D013710:Tendons / Q000033:anatomy & histology; D013997:Time Factors
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins