A deregulated immune response to gliadin causes a decreased villus height in DQ8 transgenic mice.
Celiac disease (CD) is an enteropathy triggered by gluten and mediated by CD4+ T cells. A complete understanding of CD immunopathogenesis has been hindered due to the lack of adequate in vivo models. Here, we explored the effect of the inhibition of COX by indomethacin in wheat gliadin-sensitized transgenic mice expressing the HLA-DQ8 heterodimer, a molecule associated with CD. Treated mice showed a gliadin-specific immune response with a significant reduction of villus height, not linked to crypt hyperplasia and to expansion of intraepithelial T cells. Notably, treated mice showed increased numbers of CD25+ and apoptotic cells in the lamina propria, whereas high basal levels of IFN-gamma secretion, along with a reduced gliadin-specific IL-2 expression were detected in MLN. Biochemical assessment of the lesion revealed increased mRNA of Lamb3 and Adamts2, encoding for ECM proteins, and enhanced activities of metalloproteinases MMP1, 2 and 7. We conclude that an intestinal sensitivity to gliadin, in connection with COX inhibition, caused a decreased villus height in DQ8 tg mice. The lesion was induced by a deregulated mucosal cell immunity to gliadin, thus triggering activation of a specific ECM protein pathway responsible for lamina propria remodeling.
D'Arienzo
Rossana
R
Institute of Food Sciences, CNR, Avellino, Italy.
Stefanile
Rosita
R
Maurano
Francesco
F
Luongo
Diomira
D
Bergamo
Paolo
P
Mazzarella
Giuseppe
G
Troncone
Riccardo
R
Auricchio
Salvatore
S
David
Chella
C
Rossi
Mauro
M
D051722:ADAM Proteins / Q000235:genetics; D000071096:ADAMTS Proteins; D000071121:ADAMTS4 Protein; D000818:Animals; D017209:Apoptosis; D017252:CD3 Complex / Q000378:metabolism; D002446:Celiac Disease / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005903:Gliadin / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D011348:Procollagen N-Endopeptidase / Q000235:genetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000097794:Kalinin
D051722:ADAM Proteins; D000071096:ADAMTS Proteins; D000071121:ADAMTS4 Protein; D017252:CD3 Complex; D015815:Cell Adhesion Molecules; D016861:Cyclooxygenase Inhibitors; D051858:Forkhead Transcription Factors; D005903:Gliadin; D006683:HLA-DQ Antigens; D007213:Indomethacin; D007371:Interferon-gamma; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2; D020783:Matrix Metalloproteinase 7; D011348:Procollagen N-Endopeptidase; D011451:Prostaglandin-Endoperoxide Synthases; D000097794:Kalinin; C498833:Foxp3 protein, mouse; C109756:HLA-DQ8 antigen; C507081:Mmp13 protein, mouse; C000609307:ADAMTS2 protein, human; C000609308:Adamts2 protein, mouse; C522361:MMP2 protein, human