[The comparison of the three anti-dsDNA antibody detecting test].
OBJECTIVE
To compare the three Anti-dsDNA antibody detecting test (IIF, ELISA, Farr) with 200 serum samples to evaluate which one has higher sensitivity and specificity.


METHODS
200 serum samples including 120 serum samples of SLE, 20 serum samples of rheumatoid arthritis, 20 serum samples of MCTD, 20 serum samples of SS, 20 serum samples of PSS and 50 serum samples of healthy measured by IIF, Farr and ELISA.


RESULTS
Detection the Anti-dsDNA antibody of the serum sample with the methods of IIF, ELISA and Farr. The positive percentage of Anti-dsDNA in SLE is 25%, 32% and 32%, while in RA is 0, 0 and 0; in PSS is 0, 0 and 5%; in SS is 0, 0 and 0; in healthy is 0, 0 and 0.


CONCLUSIONS
Detection the Anti-dsDNA antibody with two method in the same time, especially with IIF and ELISA, will heighten the positive rate than with single method and will be helpful for the diagnosis of SLE.
Clinical Laboratory of Peking Union Medical College Hospital, Beijing 100073, China. dawei_tong@163.com
Da-Wei
DW
Tong
Shu-Lan
SL
Zhang
Yong-Zhe
YZ
Li
Chao-Jun
CJ
Hu
D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000276:immunology; D016022:Case-Control Studies; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis* / Q000276:immunology; D011863:Radioimmunoassay
D000974:Antibodies, Antinuclear; D004247:DNA