Regulation of ANKRD9 expression by lipid metabolic perturbations.
Fatty acid oxidation (FAO) defects cause abnormal lipid accumulation in various tissues, which provides an opportunity to uncover novel genes that are involved in lipid metabolism. During a gene expression study in the riboflavin deficient induced FAO disorder in the chicken, we discovered the dramatic increase in mRNA levels of an uncharacterized gene, ANKRD9. No functions have been ascribed to ANKRD9 and its orthologs, although their sequences are well conserved among vertebrates. To provide insight into the function of ANKRD9, the expression of ANKRD9 mRNA in lipidperturbed paradigms was examined. The hepatic mRNA level of ANKRD9 was repressed by thyroid hormone (T(3)) and fasting, elevated by re-feeding upon fasting. However, ANKRD9 mRNA level is reduced in response to apoptosis. Transient transfection assay with green fluorescent protein tagged- ANKRD9 showed that this protein is localized within the cytoplasm. These findings point to the possibility that ANKRD9 is involved in intracellular lipid accumulation. [BMB reports 2009; 42(9): 568-573].
Department of Biological Sciences, Tennessee State University, 3500 John A Merritt Blvd, Nashville, TN 37221, USA. xwang@tnstate.edu
Xiaofei
X
Wang
Robert F
RF
Newkirk
Wilfrid
W
Carre
Purnima
P
Ghose
Barry
B
Igobudia
James G
JG
Townsel
Larry A
LA
Cogburn
D000563:Amebicides / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat; D017209:Apoptosis; D002642:Chick Embryo; D002645:Chickens; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005839:Gentamicins / Q000494:pharmacology; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D000563:Amebicides; D005230:Fatty Acids, Nonesterified; D005839:Gentamicins; D026901:Membrane Transport Proteins; D011506:Proteins; D012333:RNA, Messenger; C019079:riboflavin-binding protein; C010680:antibiotic G 418