Immobilization of DNA on microporous PVDF membranes by plasma polymerization.
Microporous poly(vinylidene fluoride) (PVDF) membranes with different porous surface morphologies were prepared by immersion-precipitation from coagulation baths of different strengths. On these membranes single-strand deoxyribonucleic acid (ss-DNA) was covalently immobilized by a dual-step procedure. First, poly(glycidyl methacrylate) (PGMA) was grafted on PVDF membranes by plasma-induced free radical polymerization. Then, ss-DNA was bonded to PGMA through ring-opening reactions of epoxies with amine to form amino alcohols. The highest attainable graft yield of PGMA on PVDF membrane was 0.3 mg/cm(2), which was the case when a highly porous PVDF membrane was employed as the substrate. For immobilization of ss-DNA, the yield was found to depend significantly on the reaction temperature and pH. The maximal value was 48.5 mug/cm(2). Furthermore, adsorption tests of anti-DNA antibody were carried out on membranes with and without immobilized ss-DNA using serum obtained from systemic lupus erythematosus patients. The results indicated that the immobilized DNA could effectively adsorb the antibody in the serum.
Department of Chemical and Materials Engineering, Tamkang University, Taipei 25137, Taiwan.
Dar-Jong
DJ
Lin
Dong-Tsamn
DT
Lin
Tai-Horng
TH
Young
Tsung-Chin
TC
Chen
Hsu-Hsien
HH
Chang
Liao-Ping
LP
Cheng
D000327:Adsorption; D001128:Argon / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D013696:Temperature; D005465:Fluorocarbon Polymers
D001128:Argon; D004277:DNA, Single-Stranded; D008567:Membranes, Artificial; D011109:Polymethacrylic Acids; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C042535:polyglycidyl methacrylate