[Biocompatibility of a novel cavernous nickel-titanium alloy with rat bone marrow stromal cells in vitro].
OBJECTIVE
To investigate the biocompatibility of a novel cavernous nickel-titanium alloy with rat bone marrow stromal cells (BMSCs) in vitro.


METHODS
Rat BMSCs were cultured on the surface of compact, microporous and macroporous nickel-titanium alloys, and the cell proliferation on day 3 during the culture was assessed using MTT assay. On day 7 of the cell culture, the cells were labeled with Hoechst33342 for cell counting under a fluorescence microscope. Scanning electron microscopy (SEM) was performed on day 7 of cell culture to observe the morphological changes of the cells.


RESULTS
The cell proliferation rate and cell numbers differed significantly between the cavernous alloy groups and the compact alloy group (P<0.05), but similar between the former two groups (P>0.05). SEM showed that compared with the compact alloy, microporous and macroporous nickel-titanium alloys had better biocompatibility with the BMSCs, and the cells on the surface of the cavernous alloys had normal cell morphology.


CONCLUSIONS
Cavernous nickel-titanium alloy has good biocompatibility and can promote the adhesion, aggregation and proliferation of rat BMSCs in vitro.
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China. luoj73@126.com
Jie
J
Luo
Yi-quan
YQ
Ke
Ru-xiang
RX
Xu
Xiao-dan
XD
Jiang
Shan
S
Xue
Yun
Y
Lü
Sen
S
Li
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D008297:Male; D008422:Materials Testing / Q000379:methods*; D009532:Nickel / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology*; D014025:Titanium / Q000494:pharmacology*
D001672:Biocompatible Materials; C040654:titanium nickelide; D009532:Nickel; D014025:Titanium