Osteoclasts are involved in the maintenance of dormant leukemic cells.
Osteoclasts (OCs) are specialized cells for the resorption of bone matrix that have also been recently reported to be involved in the mobilization of hematopoietic progenitor cells. When Ba/F3 cells expressing wild-type bcr-abl were co-cultured with osteoblasts (OBs), OCs, and bone slices, their proliferation was significantly suppressed, and the Ki-67 negative population, which is believed to be in G(0) phase, was increased. The results of our in vitro experiments suggest that OCs could be involved in the maintenance of dormant leukemic cells in the bone marrow (BM) microenvironment through the release of soluble factors, one of which could be TGF-beta.
Yokota
Asumi
A
Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto, Japan.
Kimura
Shinya
S
Tanaka
Ruriko
R
Takeuchi
Miki
M
Yao
Hisayuki
H
Sakai
Kazuki
K
Nagao
Rina
R
Kuroda
Junya
J
Kamitsuji
Yuri
Y
Kawata
Eri
E
Ashihara
Eishi
E
Maekawa
Taira
T
D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology; D010010:Osteoclasts / Q000378:metabolism / Q000502:physiology*; D055153:Stem Cell Niche / Q000187:drug effects / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D019394:Ki-67 Antigen; D053773:Transforming Growth Factor beta1; D016044:Fusion Proteins, bcr-abl