Abiotic stress-associated miRNAs: detection and functional analysis.
MicroRNAs (miRNAs) are small regulatory noncoding RNAs varying in length between 20 and 24 nucleotides. They play a key role during plant development by negatively regulating gene expression at the posttranscriptional level. Moreover, recent studies reported several miRNAs associated with abiotic stress responses. Small RNA cloning and high-throughput deep sequencing methods provide expression profiles of not only known miRNAs, but also novel miRNAs. In this chapter, we describe the methods used to identify and characterize abiotic stress-associated miRNAs and their target genes.
Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Dong-Hoon
DH
Jeong
Marcelo A
MA
German
Linda A
LA
Rymarquis
Shawn R
SR
Thatcher
Pamela J
PJ
Green
D003080:Cold Temperature; D019295:Computational Biology; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D010710:Phosphates / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012492:Salts / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism
D035683:MicroRNAs; D010710:Phosphates; D012492:Salts; D013431:Sulfates