[The correlation of the fetal cytokeratin expressing in epidermal cells and the different outcomes of wound repairing].
OBJECTIVE
To investigate the changing regular of specific cytokeratin (CK) markers expressing in human pseudoepitheliomatous hyperplasia (PEH), keloids (Ke) and hypertrophic scar (HS) lesion, and to explore the correlation between such changes and the different outcomes of wound repair.


METHODS
Histopathology and immunohistochemistry (IHC) double staining methods were used in samples of human PEH, Ke, HS and NS to determine the distribution characteristics and changing regularity of CKs in epidermal tissues.


RESULTS
No CK8&18 and CK17 expressed in epidermis of NS group, while CK8&18(+) cells and CK17(+) cells were detected in epidermis of active-stage Ke, HS and PEH. The quantities of CK8&18(+) cells and CK17(+) cells ranked as follows: PEH > Ke > HS and HS > Ke > PEH (P < 0.05). CK19(+) cells and CK5&6(+) cells expressed similar changing trend, while reverse trend of CK10(+) cells was detected in epidermal cells, with local epidermal hyperplasia, cells morphological changes and sub-epidermal inflammatory reaction.


CONCLUSIONS
Different degree of de-differentiation and terminal differentiation imbalance are found in epidermal cells of active-stage PEH, Ke and HS, which hint the correlation between the abnormal proliferation and differentiation of epidermal cells and the different outcomes of wound repair.
Wei
Xin-jing
XJ
Department of Dermatology, Provincial Hospital, Affiliated to Shandong University, Jinan 250021, China.
Jiang
Du-yin
DY
Zong
Xian-lei
XL
Fu
Xiao-bing
XB
Sheng
Zhi-yong
ZY
Wang
Wei
W
Shan
Fei
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D007223:Infant; D007633:Keratins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014945:Wound Healing; D055815:Young Adult
D007633:Keratins