Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4-based vaccine vector development.
In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development.
Department of Animal Health, Sezione di Malattie Infettive degli Animali, Facoltà di Medicina Veterinaria, University of Parma, Parma, Italy. gaetano.donofrio@unipr.it
Gaetano
G
Donofrio
Valentina
V
Franceschi
Antonio
A
Capocefalo
Simone
S
Taddei
Chiara
C
Sartori
Sabrina
S
Bonomini
Sandro
S
Cavirani
Clotilde S
CS
Cabassi
Cesidio F
CF
Flammini
D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D015478:Antigens, Heterophile / Q000276:immunology / Q000378:metabolism*; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology; D026021:Herpesvirus 4, Bovine / Q000235:genetics / Q000276:immunology*; D021381:Protein Transport; D011817:Rabbits; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*; D014765:Viral Vaccines / Q000276:immunology*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015478:Antigens, Heterophile; D014764:Viral Proteins; D014765:Viral Vaccines