[Determination of xanthohumol in beer by solid-phase extraction-high performance liquid chromatography].
A method for the determination of xanthohumol in beer using solid-phase extraction-high performance liquid chromatography (SPE-HPLC) has been developed. A Waters Sep-Pak C18 column was used to extract and clean-up the sample. The separation was achieved on a reversed-phase Agilent Zorbax Eclipse XDB-C18 (250 mm x 4.6 mm, 5 microm) in a linear gradient, and the mobile phases were consisted of water (containing 0.1% formic acid) (A) and methanol (B) with a flow rate of 0.4 mL/min. In addition, the column temperature was maintained at 25 degrees C. The detection wavelength was set at 370 nm. There was a good linear relationship (r2 = 1) in the range of 0.5 - 500 microg/L. The average recoveries were between 91.21% and 95.58% with the relative standard deviations less than 2%. The limit of detection was 0.24 microg/L and the limit of quantification was 0.80 microg/L. It was proved to be a convenient and accurate method for the analysis of xanthohumol content in beer.
Wang
Ning
N
Jiangnan University, Key Laboratory of Industrial Biotechnology of Ministry of Education, Wuxi 214122, China.
Li
Yongxian
Y
Zheng
Feiyun
F
Liu
Chunfeng
C
Li
Qi
Q
Gu
Guoxian
G
D001515:Beer / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D011427:Propiophenones / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*
D005419:Flavonoids; D011427:Propiophenones; C104536:xanthohumol