Short-term effects of zinc on acetylcholine metabolism and viability of SN56 cholinergic neuroblastoma cells.
Excessive accumulation of zinc in the brain is one of putative factors involved in pathomechanism of cholinergic encephalopathies. The aim of this work was to investigate whether short-term increase of zinc concentration in the extracellular space may affect energy and acetylcholine metabolism in SN56 cholinergic cells of septal origin. Short 30 min exposition of SN56 cells to increasing zinc levels caused greater loss of viability of differentiated (DC, [EC(0.4)] 0.09 mM) than nondifferentiated cells (NC, [EC(0.4)] 0.14 mM). Concentration-dependent accumulation of zinc displayed exponential non-saturable kinetics. Zinc accumulation caused the decrease of calcium accumulation in mitochondria and its increase in cytoplasmic compartment of SN56 cells. Significant inverse and direct correlations were found between zinc accumulation and calcium levels in mitochondrial (r=-0.96, p=0.028) and cytoplasmic (r=0.97, p=0.028) compartments of DC, respectively. Zinc exerted similar inhibition of pyruvate dehydrogenase, aconitase and isocitrate dehydrogenase both in NC and DC homogenates, at Ki values equal to about 0.07, 0.08 and 0.005 mM, respectively. On the other hand, ketoglutarate dehydrogenase activity in DC was inhibited by zinc (Ki 0.0005 mM) 8 times stronger that in NC (Ki 0.004 mM). Also zinc-evoked decreases in acetylcholine content and its release were significantly greater in DC than in NC. Same conditions caused suppression of cytoplasmic and mitochondrial content of acetyl-CoA, that positively correlated with inhibition of transmitter functions (r=0.995, p=005) and loss of cell viability (r=0.990, p=0.0006), respectively. Significant correlations were also found in zinc-challenged cells between pyruvate dehydrogenase activity and both mitochondrial acetyl-CoA content and cell viability. These data indicate that pyruvate dehydrogenase-dependent acetyl-CoA synthesis in neuronal mitochondria may be a primary target for short-term neurotoxic effects of zinc. In consequence, shortages of acetyl-CoA in the mitochondrial compartment would cause fast loss of functional and structural integrity of cholinergic neurons.
Department of Laboratory Medicine, Medical University of Gdańsk, Gdansk, Poland.
Anna
A
Ronowska
Aleksandra
A
Dyś
Agnieszka
A
Jankowska-Kulawy
Joanna
J
Klimaszewska-Łata
Hanna
H
Bielarczyk
Piotr
P
Romianowski
Tadeusz
T
Pawełczyk
Andrzej
A
Szutowicz
D000105:Acetyl Coenzyme A / Q000096:biosynthesis*; D000109:Acetylcholine / Q000378:metabolism*; D000154:Aconitate Hydratase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002799:Cholinergic Fibers / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects* / Q000502:physiology; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology; D007521:Isocitrate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009498:Neurotoxins / Q000633:toxicity; D011768:Pyruvate Dehydrogenase Complex / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D015032:Zinc / Q000633:toxicity*
D009498:Neurotoxins; D011768:Pyruvate Dehydrogenase Complex; D000105:Acetyl Coenzyme A; D007521:Isocitrate Dehydrogenase; D000154:Aconitate Hydratase; D015032:Zinc; D000109:Acetylcholine; D002118:Calcium