Effect of baicalin and octreotide on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis.
OBJECTIVE
To investigate the effect of baicalin and octreotide on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis and explore the underlying mechanism.


METHODS
Rats were randomly divided into sham-operated, model control, baicalin-treated and octreotide-treated groups. At 3, 6 and 12 h after operation, the mortality rates of rats, the contents of plasma endotoxin as well as serum NO and ET-1, the pathological changes in multiple organs, and the expression levels of P-selectin protein in each group were observed.


RESULTS
At 12 h after operation, the mortality rates of rats in treated groups were significantly lower than that in the model control group (P < 0.05), and the pathological severity scores in multiple organs in treated groups were also significantly lower than those in the model control group (P < 0.05). The contents of plasma endotoxin, serum PLA(2) (at 6 and 12 h after operation), ET-1 and NO (at 3 and 12 h after operation) in treated groups were significantly lower than those in the model control group (P < 0.05, P < 0.01 or P < 0.001). In the baicalin-treated group, the expression levels of P-selectin protein in liver (at 3 h after operation), kidney (at 3 and 6 h after operation), pancreas, lung and spleen were significantly lower than those in the model control group (P < 0.01). In the octreotide-treated group, the expression levels of this protein in lung, intestinal mucosa (at 6 and 12 h after operation), lymph nodes (at 3 and 6 h after operation), spleen and thymus were significantly lower than those in the model control group (P < 0.05). Additionally, the products of the staining intensity and positive rate of P-selectin protein in pancreas, spleen (at 3 h after operation), intestinal mucosa (at 6 h after operation), thymus (at 6 h after operation) and lung (at 6 h after operation) in treated groups were significantly lower than those in the model control group (P < 0.05).


CONCLUSIONS
Both baicalin and octreotide can exert some protective effects on multiple organs and the former is superior to the latter in protecting pancreas. Furthermore, decreasing the expression levels of P-selectin protein in these organs is one of the possible mechanisms.
Department of General Surgery, Hangzhou First People's Hospital, China. zxp99688@vip.163.com
Xiping
X
Zhang
Guanghua
G
Feng
Jun
J
Jiang
Hua
H
Tian
Li
L
Chen
Yang
Y
Cai
Yuemin
Y
Shou
Jin
J
Ma
Meili
M
Ma
Feibo
F
Zhu
D000208:Acute Disease; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood; D004731:Endotoxins / Q000097:blood; D005419:Flavonoids / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009569:Nitric Oxide / Q000097:blood; D015282:Octreotide / Q000494:pharmacology*; D019007:P-Selectin / Q000378:metabolism*; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D054467:Phospholipases A2 / Q000097:blood; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D013656:Taurocholic Acid; D013997:Time Factors; D046888:Tissue Array Analysis
D015415:Biomarkers; D019332:Endothelin-1; D004731:Endotoxins; D005419:Flavonoids; D019007:P-Selectin; D020011:Protective Agents; D009569:Nitric Oxide; C038044:baicalin; D013656:Taurocholic Acid; D054467:Phospholipases A2; D015282:Octreotide