11-Tungstophosphate with iron(II) and hydrogen peroxide efficiently detached bacterial biofilm.
An attempt was made to detach bacterial biofilm, formed by Staphylococcus epidermidis, by using hydrogen peroxide (H(2)O(2)) and tungsten compounds. When iron(II) (Fe(2+)) was mixed with undecatungstophosphate ([PW(11)O(39)](7-)) and then H(2)O(2), the resulting mixture was able to totally remove the biofilm probably because of co-generation of (1)O(2) and .OH. A mixture of undecatungstosilicate ([SiW(11)O(39)](8-)) and Fe(2+) (or Cu(2+)) also gave a good result, but their catalytic activities for producing .OH (or (1)O(2)) were rather weak. An electron microscopic study showed that almost nothing was visible on the surface of a biofilm-coated glass after treatment with 1mM [PW(11)O(39)](7-)+1 mM Fe(2+) and 500 mM H(2)O(2) (incubated for 1 h at 37 degrees C).
Tajima
Yutaka
Y
Legal Medicine and Molecular Genetics, Postgraduate School of Medicine, Gunma University, Maebashi, Japan. y-tajima@koaikai.or.jp
Takagi
Rie
R
Nakajima
Tamiko
T
Yasuda
Toshiro
T
Kominato
Yoshihiko
Y
D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology*; D010772:Phosphotungstic Acid / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000502:physiology
D005296:Ferrous Compounds; D010772:Phosphotungstic Acid; D006861:Hydrogen Peroxide; D007501:Iron