Comparison of changes in markers of muscle damage induced by eccentric exercise and ischemia/reperfusion.
To examine the effects of eccentric exercise (EE) and ischemia/reperfusion (I/R) on the markers of muscle damage, 72 rats were randomly assigned to the EE group, I/R group and control group (C), respectively. The rats in EE ran downhill on a treadmill with a 16 ° inclination at a constant speed for 90 min, and the rats in the I/R group underwent 90 min of four-limb ischemia, followed by 24, 48 and 72 h of reperfusion. Blood and tissue samples were collected immediately, 24, 48 and 72 h after exercise or reperfusion. Quantitative analyses showed that the I/R group had a significantly larger mitochondrial volume at 24 h after reperfusion compared with the C, and there were more disrupted Z-lines in the EE group and more disrupted mitochondria in the I/R group at 24 h after exercise or reperfusion. When compared with the C, a significantly lower total antioxidant capacity and higher interleukin-6 value were observed after exercise or reperfusion. Our data suggest that although EE and I/R result in some similar changes in the muscle damage markers, there are still some differences. The EE- and I/R-induced muscle damage may be due to different mechanisms.
Faculty of Sports Medicine, Chengdu Sports University, Chengdu, China.
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Su
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JG
Zhang
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Dong
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Hua
J-Z
JZ
Sun
D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D003402:Creatine Kinase / Q000097:blood; D052283:Creatine Kinase, MM Form / Q000097:blood; D005260:Female; D015850:Interleukin-6 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008931:Mitochondria, Muscle / Q000648:ultrastructure*; D009132:Muscles / Q000293:injuries* / Q000378:metabolism / Q000648:ultrastructure; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology*; D013482:Superoxide Dismutase / Q000097:blood
D015415:Biomarkers; D015850:Interleukin-6; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D052283:Creatine Kinase, MM Form