Effects of hyaluronic acid sponge as a scaffold on odontoblastic cell line and amputated dental pulp.
It is important to develop a suitable three-dimensional scaffold for the regeneration therapy of dental pulp. In the present study, the effects of hyaluronic acid (HA) sponge on responses of the odontoblastic cell line (KN-3 cells) in vitro, as well as responses of amputated dental pulp of rat molar in vivo, were examined. In vitro, KN-3 cells adhered to the stable structure of HA sponge and that of collagen sponge. In vivo, dental pulp proliferation and vessel invasion were observed in both sponges implanted at dentin defect area above amputated dental pulp, and the cell-rich reorganizing tissue was observed in the dentin defect when HA sponge was implanted as compared with collagen sponge. Expression levels of IL-6 and TNF-alpha in KN-3 cells seeded in HA sponge were nearly the same with those in the cells seeded in collagen sponge, while the numbers (0.67 x 10(3) at 1 week and 0.7 x 10(3) at 3 weeks) of granulated leukocytes that invaded into HA sponge from amputated dental pulp was significantly lower than those (1.22 x 10(3) at 1 week and 1.1 x 10(3) at 3 weeks) of collagen sponge (p < 0.01 at 1 week and p < 0.05 at 3 weeks). These results suggest that HA sponge has an appropriate structure, biocompatibility, and biodegradation for use as a scaffold for dental pulp regeneration.
Division of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Kyushu Dental College, Japan.
Yoshio
Y
Inuyama
Chiaki
C
Kitamura
Tatsuji
T
Nishihara
Takahiko
T
Morotomi
Masato
M
Nagayoshi
Yasuhiko
Y
Tabata
Kou
K
Matsuo
Ker-Kong
KK
Chen
Masamichi
M
Terashita
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D003782:Dental Pulp / Q000166:cytology*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009804:Odontoblasts / Q000166:cytology*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D006820:Hyaluronic Acid