Heat-shock factor 1 controls genome-wide acetylation in heat-shocked cells.
A major regulatory function has been evidenced here for HSF1, the key transcription factor of the heat-shock response, in a large-scale remodeling of the cell epigenome. Indeed, upon heat shock, HSF1, in addition to its well-known transactivating activities, mediates a genome-wide and massive histone deacetylation. Investigating the underlying mechanisms, we show that HSF1 specifically associates with and uses HDAC1 and HDAC2 to trigger this heat-shock-dependent histone deacetylation. This work therefore identifies HSF1 as a master regulator of global chromatin acetylation and reveals a cross-talk between HSF1 and histone deacetylases in the general control of genome organization in response to heat shock.
Institut National de la Santé et de la Recherche Médicale, U823, Institut Albert Bonniot, Université Joseph Fourier, F-38706 Grenoble, France.
Sabrina
S
Fritah
Edwige
E
Col
Cyril
C
Boyault
Jérôme
J
Govin
Karin
K
Sadoul
Susanna
S
Chiocca
Elisabeth
E
Christians
Saadi
S
Khochbin
Caroline
C
Jolly
Claire
C
Vourc'h
D000107:Acetylation; D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D016678:Genome; D000076249:Heat Shock Transcription Factors; D018869:Heat-Shock Response / Q000235:genetics*; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism; D056464:Histone Deacetylase 2 / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D002843:Chromatin; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D006657:Histones; C490017:Hsf1 protein, mouse; D034741:RNA, Small Interfering; D014157:Transcription Factors; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2