Durable, region-specific protein patterning in microfluidic channels.
We present a straightforward, accessible method to covalently pattern proteins in poly(dimethyl siloxane) (PDMS) microchannels. Our approach includes (i) region-specific photografting of a layer of poly(acrylamide) (PAAm) and (ii) bioconjugation of PAAm with a desired protein. The method produces symmetric protein patterns on all channel walls, which have high specificity and pattern fidelity, are compatible with a variety of geometries and exhibit excellent longevity under shear stresses of up to 1 dyn/cm. We demonstrate the generality of the method by creating multi-protein gradients within microfluidic microchannels and by in-situ patterning of islands of multiple proteins. Protein activity was observed by the digestion of BODIPY-casein using channels patterned with trypsin.
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.
Lindsey K
LK
Fiddes
Ho Ka C
HK
Chan
Bryan
B
Lau
Eugenia
E
Kumacheva
Aaron R
AR
Wheeler
D002364:Caseins / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate; D005453:Fluorescence; D044085:Microfluidics / Q000379:methods*; D040081:Protein Array Analysis; D013499:Surface Properties / Q000528:radiation effects; D014357:Trypsin / Q000378:metabolism; D014466:Ultraviolet Rays
D002364:Caseins; D004129:Dimethylpolysiloxanes; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate