Oriented immobilization of antibody fragments on Ni-decorated single-walled carbon nanotube devices.
We herein demonstrate that Ni-decorated single-walled carbon nanotube field effect transistors (SWNT-FETs) combined with antibody fragments can be used as effective biosensing platforms. Nanoscales Ni particles 20 to 60 nm in diameter were formed on the sidewalls of SWNT-FETs using an electrochemical method. Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)(6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. Whereas randomly oriented anti-CEA scFvs did not impart a noticeable change of conductance upon addition of CEA, a clear increase in conductance was observed using Ni-decorated SWNT-FETs functionalized with engineered scFvs.
Lo
Young-Seop
YS
NanoBio Fusion Research Center, Korea Research Institute of Chemical Technology, Daejeon, Korea.
Nam
Dong Hyun
DH
So
Hye-Mi
HM
Chang
Hyunju
H
Kim
Ju-Jin
JJ
Kim
Yong Hwan
YH
Lee
Jeong-O
JO
D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002417:Cattle; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D006639:Histidine / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D009842:Oligopeptides / Q000378:metabolism; D011487:Protein Conformation; D015202:Protein Engineering; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014173:Transistors, Electronic
D055910:Antibodies, Immobilized; D002272:Carcinoembryonic Antigen; C471213:His-His-His-His-His-His; D037742:Nanotubes, Carbon; D009842:Oligopeptides; D057127:Single-Chain Antibodies; D006639:Histidine; D006046:Gold; D009532:Nickel