Assessment of cadmium genotoxicity in peripheral blood and bone marrow tissues of male Wistar rats.
In this study, Cadmium (Cd) genotoxicity was investigated in both bone marrow and peripheral blood treatment using rat micronucleus technique as genotoxicity test at acute and chronic treatment in the same animals. This study evaluated the frequency of micronuclei in the peripheral blood and bone marrow of male rats treated with unique cadmium dose (15 mg/kg. body w/day) by gavage for 60 days and acute treatment for 24 h, respectively. Mitomycin C (MMC) 2 mg/kg body wt was used as a positive control. This study shows that cadmium chloride treatment significantly induced the frequency of micronucleus in polychromatic erythrocytes in both tibia bone marrow and peripheral blood (p < 0.001, p < 0.01, respectively). This increase in micronucleus frequency shows that cadmium has a genotoxic effect on bone marrow and peripheral blood at this level. Also, in order to determine cytotoxicity in bone marrow and peripheral blood, the ratio of polychromatic erythrocytes to normochromatic erythrocytes was calculated in bone marrow and peripheral blood. Cd treatment decreased this ratio in only bone marrow. The results of this study demonstrate that Cd has both toxic and genotoxic potential in bone marrow and only genotoxic potential in peripheral blood. There is a significant difference between the control group and exposed group, including acute and chronic treatment for blood Cd level (p < 0.001). No significant difference was found between acute and chronic exposure group (p > 0.05).
Celik
Ayla
A
Department of Biology, Faculty of Science and Letters, Mersin University, Mersin, Turkey.
Büyükakilli
Belgin
B
Cimen
Burak
B
Taşdelen
Bahar
B
Oztürk
M Ikbal
MI
Eke
Dilek
D
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D019256:Cadmium Chloride / Q000633:toxicity*; D004247:DNA / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D016685:Mitomycin / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D004247:DNA; D019256:Cadmium Chloride