[Isolation, identification and diversity analysis of petroleum-degrading bacteria in Shengli Oil Field wetland soil].
The petroleum-degrading bacteria in Shengli Oil Field wetland soil were isolated and identified by traditional experiment methods, and their diversity was analyzed by PCR-DGGE (denaturing gradient gel electrophoresis). A total of thirteen petroleum-degrading bacterial strains were isolated, among which, six strains were found to have the ability of degrading the majority of C12-C26 petroleum hydrocarbon, with a degradation rate of > 90%. These petroleum degraders were phylogeneticly identified as the members of Halomonas, Alcanivorax, and Marinobacter, which were all belonged to gamma-proteobacteria. The uncultured predominant bacteria in Shengli Oil Field wetland soil were of Sulfurovum, Gillisia and Arcobacter. Among the predominant bacteria, gamma-proteobacteria accounted for a larger proportion, followed by alpha-proteobactiria, epsilon-proteobactiria, Actinobacteria, and Flavobacteria.
Research Centre for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, Shandong, China. han_ping89@yahoo.com.cn
Ping
P
Han
Li
L
Zheng
Zhi-Song
ZS
Cui
Xiu-Chun
XC
Guo
Li
L
Tian
D020561:Alphaproteobacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D002681:China; D020563:Gammaproteobacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism*; D012988:Soil Microbiology; D053833:Wetlands
D010578:Petroleum