[Effects of Annexin II gene silencing by siRNA on proliferation and invasive potential of Jurkat lymphoma cells].
OBJECTIVE
To investigate the effects of Annexin II (AnxA2) gene silencing by siRNA on proliferation and invasive potential of lymphoma cell line Jurkat cells.


METHODS
A synthesized siRNA duplex targeting to AnxA2 was transfected into Jurkat cells. Transfection efficiency was analyzed by real-time PCR and flow cytometry. MTT assay for cell proliferation and transwell plates for invasive potential were performed.


RESULTS
Compared with the negative controls, the cell proliferation inhibitory rate of the AnxA2 siRNA transfected Jurkat cells was significantly increased at 24 h, 48 h and 72 h [(17.4 +/- 2.3)%, (22.4 +/- 3.8)%, (37.6 +/- 1.5)% vs (-1.3 +/- 5.1)%, (-5.5 +/- 4.4)%, (-10.8 +/- 5.5)%, respectively, P<0.05]. The cell invasive potential of the transfected Jurkat cells was inhibited remarkably at 48 h (11.3 +/- 4.2 vs 54.3 +/- 8.7, P<0.01).


CONCLUSIONS
AnxA2 gene silenced by siRNA can inhibit the proliferation and the invasive potential of Jurkat cells remarkably.
The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Soochow 215007, China.
Hong-yu
HY
Bao
Miao
M
Jiang
Zhen-ni
ZN
Ma
Fei
F
Sheng
Ming-qing
MQ
Zhu
Lin
L
Chen
Li-qian
LQ
Xie
Ning-zheng
NZ
Dong
Chang-geng
CG
Ruan
D017306:Annexin A2 / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D002633:Chemotaxis / Q000235:genetics; D020868:Gene Silencing; D006801:Humans; D019169:Jurkat Cells; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D017306:Annexin A2; D034741:RNA, Small Interfering