Genome-wide high-resolution chromatin immunoprecipitation of meiotic chromosomal proteins in Saccharomyces cerevisiae.
Cooperative actions of chromosomal proteins play critical roles in the dynamics, structural transition, segregation, and maintenance of meiotic chromosomes. A high-resolutibn genome-tiling array combined with a chromatin immunoprecipitation assay (ChIP-chip) is a powerful tool for uncovering the precise and dynamic distributions of various proteins along meiotic chromosomes. In this chapter, we describe a method to map the binding sites of meiotic chromosomal proteins such as Spo11, Mre11, and Rec8 using the high-resolution ChIP-chip technology. This system provides us with informative knowledge on the interplay of meiotic chromosomal proteins.
Kugou
Kazuto
K
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
Ohta
Kunihiro
K
D000465:Algorithms; D018929:Cell Culture Techniques / Q000379:methods; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods*; D016681:Genome, Fungal; D008540:Meiosis / Q000235:genetics*; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone