[In vitro study on the effect of ribavirin reducing the EV71 replication].
OBJECTIVE
Observe the effect of Ribavirin on reducing the EV71 replication, inactivating EV71 and protecting the RD-A cells against the EV71 infection in vitro.


METHODS
Using the EV71 isolated from Anhui Fuyang HFMD outbreak, the effect of Ribavirin on RD-A cells during the EV71 infection was observed.


RESULTS
In the experiment of Ribavirin inhibiting the EV71 replication, comparing with the no-Ribavirin-dealed virus control group, the group of 1:640 dilution of Ribavirin delayed the CPE for 2 days, while the normal cell group was growing very well. In the experiment of protecting cell from EV71 infection, comparing with the no-Ribavirin-dealed virus control group, the group of 1:8 dilution of Ribavirin delayed the CPE for 4 days. In the experiment of Ribavirin effect on the inactivation of EV71, the group of 1:40 dilution of Ribavirin delayed the CPE for 2 days comparing with the virus control group.


CONCLUSIONS
Ribavirin could inhibit the replication of the EV71 and inactivate the EV71 in vitro. Additionally, Ribavirin could protect RD-A cells from EV 71 infection. The observation will contribute to EV71 infection control and quick medicine therapy.
Zhang
Yan
Y
National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100050, China.
Zhu
Zhen
Z
Zhu
Shuang-Li
SL
An
Hong-Qiu
HQ
Yang
Zhao-Hui
ZH
Guo
Xue-Bin
XB
Xu
Wen-Bo
WB
D002460:Cell Line; D004770:Enterovirus / Q000187:drug effects* / Q000502:physiology*; D004769:Enterovirus Infections / Q000821:virology*; D006801:Humans; D012254:Ribavirin / Q000494:pharmacology*; D038301:Virus Inactivation / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
D012254:Ribavirin