Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B12 hydrolysate on alpha1-acid glycoprotein and beta-cyclodextrin columns.
Taking into account the structural similarities of amino alcohols with amino acids and in order to reduce time for derivatization unconventional approaches viz. microwave irradiation, ultrasonication and ultra centrifugation were applied for synthesis of dinitrophenyl derivatives of nine amino alcohols and to work out a method of choice for sample preparation for direct enantioseparation. The enantiomeric dinitrophenyl derivatives so synthesized were separated on alpha(1)-acid glycoprotein and beta-cyclodextrin columns with detection at 230nm using photodiode array detection system. Derivatization methods and chromatographic parameters were optimized. beta-Cyclodextrin column was found better compared to AGP column for enantioseparation. Limit of detection, quantification, accuracy and precision were also determined. The developed method was successfully applied to determine enantiomeric purity of 1-amino-2-propanol obtained from vitamin B(12) hydrolysate.
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India. rbushfcy@iitr.ernet.in
Ravi
R
Bhushan
Rajender
R
Kumar
D000605:Amino Alcohols / Q000737:chemistry / Q000302:isolation & purification*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D004136:Dinitrobenzenes / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D006358:Hot Temperature; D008872:Microwaves; D011412:Propanolamines / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*; D013010:Sonication; D013237:Stereoisomerism; D014461:Ultracentrifugation; D014805:Vitamin B 12 / Q000032:analysis; D047392:beta-Cyclodextrins / Q000737:chemistry
D000605:Amino Alcohols; D004136:Dinitrobenzenes; D006023:Glycoproteins; D011412:Propanolamines; D011492:Protein Hydrolysates; D047392:beta-Cyclodextrins; C031215:betadex; D014805:Vitamin B 12; C008544:monoisopropanolamine