Inhibitory effect and transcriptional impact of berberine and evodiamine on human white preadipocyte differentiation.
It has been reported that the botanical alkaloids, berberine and evodiamine inhibit mouse preadipocyte 3T3-L1 differentiation. The aim of this study was to investigate the effect and transcriptional impact of berberine and evodiamine individually and in combination on human white preadipocyte (HWP) differentiation. We have shown that treatment with 8 microM berberine or 4 microM evodiamine resulted in a major inhibition of HWP differentiation accompanied by up-regulation of both GATA binding protein 2 and 3 (GATA-2 and GATA-3) mRNA and protein expression, suggesting that both compounds may have excellent potential as agents to prevent obesity.
Department of Pharmaceutical Sciences, College of Pharmacy, South Dakota State University, Brookings, SD 57007, USA.
Yueshan
Y
Hu
Hesham
H
Fahmy
Jordan K
JK
Zjawiony
Gareth E
GE
Davies
D017667:Adipocytes / Q000187:drug effects*; D052436:Adipose Tissue, White / Q000166:cytology / Q000187:drug effects*; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001599:Berberine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D050989:GATA2 Transcription Factor / Q000235:genetics / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D011799:Quinazolines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
D019440:Anti-Obesity Agents; D050989:GATA2 Transcription Factor; D050990:GATA3 Transcription Factor; D010936:Plant Extracts; D011799:Quinazolines; D012333:RNA, Messenger; D001599:Berberine; C049639:evodiamine