Experimental studies on imaging of infected site with (99m)Tc-labeled ciprofloxacin in mice.
BACKGROUND
Bacterial infection can pose a substantial diagnostic dilemma. (99m)Tc-labeled ciprofloxacin (CPF) was developed as a biologically active radiopharmaceutical to diagnose infection. In the present research, we studied the biodistribution and imaging properties of infection tracer (99m)Tc-CPF in a mouse model of infection.


METHODS
CPF was labeled with (99m)Tc and the radiochemical purity and labeling rate were measured. A mouse model of infection was established. We then determined the biodistribution of (99m)Tc-CPF and conducted the whole body scintigraphy of the animal model.


RESULTS
(99m)Tc-Ciprotech was stable for at least 6 hours at room temperature. The labeling rate of CPF by (99m)Tc was over 90%. Clearance of radioactivity mainly occurred in the liver and kidney, and the clearance from blood was rapid. Both biodistribution and imaging results showed higher uptake of (99m)Tc-CPF at sites of infection. The infectious tissue/normal tissue ratio peak was 4.30 at 4 hours after injection.


CONCLUSIONS
(99m)Tc-CPF is a sensitive radiopharmaceutical for scintigraphy of infectious lesions and it is easy to prepare.
Department of Nuclear Medicine, Second Affiliated Hospital of Sun Yat-Sen University, Guangdong, China.
Hong
H
Zhang
Ning-yi
NY
Jiang
Lin
L
Zhu
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000493:pharmacokinetics*; D001424:Bacterial Infections / Q000175:diagnosis*; D002939:Ciprofloxacin / Q000737:chemistry* / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D007553:Isotope Labeling / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D015609:Organotechnetium Compounds / Q000737:chemistry*; D014018:Tissue Distribution
D000890:Anti-Infective Agents; D015609:Organotechnetium Compounds; D002939:Ciprofloxacin