Adiponectin gene single-nucleotide polymorphisms and treatment response to obesity.
BACKGROUND
In the adiponectin gene polymorphisms, single-nucleotide polymorphism (SNP)-45 and SNP276 have reportedly been associated with obesity, Type 2 diabetes, and other features of metabolic syndrome.


OBJECTIVE
Whether these adiponectin SNP affect obesity-related parameters during caloric restriction in obese subjects.


METHODS
Thirty- two obese Japanese women were treated by meal replacement with a low calorie diet for 8 weeks and asked to maintain their habitual lifestyle. Obesity-related parameters were measured before and after the treatment period. We determined four SNP (T45G, I164T, G276T, and C-11377G) using a fluorescent allele-specific DNA primer assay systemand FRET probe assay system.


RESULTS
After the treatment, the extent of decrease in waist circumference was greater in the subjects with the G/G or G/T genotype of SNP276 than in those with the T/T genotype (p=0.026). As for SNP45, the extent of decrease in triglyceride levels was greater in the subjects with the T/T genotype than in those with the T/G genotype (p=0.003). For SNP-11377, the extent of decrease in systolic blood pressure and fasting plasma glucose was greater in the subjects with the C/G or G/G genotype than in those with the C/C genotype (p=0.044).


CONCLUSIONS
Our findings indicate that each SNP in the adiponectin gene might modify the change in obesity-related parameters during meal replacement with a low calorie diet.
Division of Preventive Medicine and Diabetes Education, Clinical Research Institute for Endocrine and Metabolic Disease, National Hospital Organization Kyoto Medical Center, Kyoto, Japan.
K
K
Tsuzaki
K
K
Kotani
N
N
Nagai
K
K
Saiga
Y
Y
Sano
T
T
Hamada
T
T
Moritani
M
M
Yoshimura
K
K
Egawa
C
C
Horikawa
Y
Y
Kitagawa
Y
Y
Kiso
N
N
Sakane
D052242:Adiponectin / Q000235:genetics; D000328:Adult; D004038:Diet, Reducing; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D016896:Treatment Outcome; D055105:Waist Circumference / Q000235:genetics
C499918:ADIPOQ protein, human; D052242:Adiponectin