Rapid noninvasive optical imaging of tissue composition in breast tumor margins.
BACKGROUND
In women undergoing breast conserving surgery (BCS), up to 60% can require re-excision. Our objective is to develop an optically based technology which can differentiate benign from malignant breast tissues intraoperatively through differences in tissue composition factors.


METHODS
A prospective study of optical imaging of BCS margins is being performed. Optical images are transformed into tissue composition maps with parameters of total hemoglobin concentration, b-carotene concentration and scattering. The predicted outcome is then compared to the margin-level pathology.


RESULTS
Fifty-five margins from 48 patients have undergone assessment. Within 34 specimens with pathologically confirmed positive margins, the ratio map of b-carotene/scattering showed the most significant difference reflecting a decrease in adipose and an increase in cell density within malignant margins (p=.002). These differences were notable in both in-situ and invasive disease.


CONCLUSIONS
We present a novel optical spectral imaging device that provides a rapid, non-destructive assay of the tissue composition of breast tumor margins.
Wilke
Lee G
LG
Department of Surgery, Duke University Health System, 3116 North Duke St., Durham, NC 27704, USA. wilke031@mc.duke.edu
Brown
J Quincy
JQ
Bydlon
Torre M
TM
Kennedy
Stephanie A
SA
Richards
Lisa M
LM
Junker
Marlee K
MK
Gallagher
Jennifer
J
Barry
William T
WT
Geradts
Joseph
J
Ramanujam
Nimmi
N
D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D015412:Mastectomy, Segmental; D055096:Optical Devices; D011237:Predictive Value of Tests; D012086:Reoperation; D013057:Spectrum Analysis