[Screening and characteristics of normal temperature lignocellulose-degradation microbial community].
A normal temperature lignocellulose-degrading microflora has been constructed by our laboratory. We researched the degradation activity and compose of the community in 28 degrees C fermentation condition. The results showed that the microbial community could degrade 39.6% of rice stew gross weight within five days.The volatile products were detected using CP-Chirasil-Dex CB capillary column by GC-MS, propionic acid, ethanol, isopropyl alcohol, 4-amino-1-butanol, butanoic acid, diethoxydimethyl-silane, lactic acid, ethanol,2,2'-oxybis-, diethyl phthalate and glycerin,more than 10 kinds of volatile products were detected. The state volatile products of changed largely along with the process of decompose, the productions gradually increase, and the content changes much with the process of decompose. Denaturing gradient gel electrophoresis (DGGE) detected the dynamic change of bacterium compose, the bacterium changes much in different period,the result of Blast from 16S rDNA sequence was found that the closest relative in community belong to Clostridium sp., Brevibacillus sp., Rhizobium sp., Bacterium sp. four genera.
Liu
Chang-li
CL
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China. liuchangli08@yahoo.com.cn
Zhu
Wan-bin
WB
Guo
Peng
P
Wang
Xiao-juan
XJ
Wang
Xiao-fen
XF
Cui
Zong-jun
ZJ
D001673:Biodegradation, Environmental; D001951:Brevibacterium / Q000302:isolation & purification / Q000378:metabolism*; D003013:Clostridium / Q000302:isolation & purification / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D012037:Refuse Disposal / Q000379:methods; D012231:Rhizobium / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
C036909:lignocellulose; D008031:Lignin