Platinum nanocolloid-supplemented hydrogendissolved water inhibits growth of human tongue carcinoma cells preferentially over normal cells.
OBJECTIVE
Hydrogen-dissolved water (HD-water) or platinum nanocolloid (Pt-nc) has been individually expected as a new therapeutic agent for oxidative stress-related diseases, whereas little is known about their combined effects on cancer, which were elucidated in the present study.


METHODS
HD-water was prepared by microporous gas bubbling, and supplemented with Pt-nc consisting of 0.003-1 ppm Pt and PVP polymers. Antioxidant activities were examined by 1, 1-diphenyl-picrylhydrazyl (DPPH)-radicalscavenging assay. Cytotoxic activities were examined by culturing of tumor and normal cell lines, respectively.


RESULTS
HD-water accelerated the Pt-nc-based DPPH-radical scavenging. Pt-nc-supplemented HD-water inhibited either colony formation efficiencies or colony sizes of human tongue carcinoma cells HSC-4, in contrast to no effects of HD-water alone, Pt-nc alone or Pt-absent PVP, but not appreciably inhibit normal human tongue epithelial-like cells DOK. Pt-nc-supplemented HD-water also suppressed cell population growth of HSC-4 cells of near-confluence (at higher cell densities) in view of decreases in either cell numbers or mitochondrial function, although less markedly than colony formation starting from a sparse-cell state (at lower cell densities). Dissolved hydrogen, oxygen concentration or oxido-reduced potentials of HD-water was decreased, rather decreased or increased by Pt-nc addition, respectively.


CONCLUSIONS
Anti-cancer activity of Pt-nc-supplemented HD-water was shown by its preferential cell-growth inhibition to human tongue carcinoma cells HSC-4 over normal human tongue cells DOK, and might be partly attributed to HD-water-caused enhancement of Pt-nc-relevant antioxidant ability. Pt-nc-supplemented HD-water is expected as a novel agent against human tongue cancers due to its cancer progression-repressive abilities.
Cell-Death Control BioTechnology Laboratory, Faculty of Life and environmental Sciences, Prefectural, University of Hiroshima, Shobara, Hiroshima, Japan.
Y
Y
Saitoh
Y
Y
Yoshimura
K
K
Nakano
N
N
Miwa
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001713:Biphenyl Compounds; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003102:Colloids / Q000737:chemistry; D003114:Colony-Forming Units Assay; D016166:Free Radical Scavengers; D006801:Humans; D006859:Hydrogen / Q000737:chemistry*; D053768:Metal Nanoparticles; D010851:Picrates; D010984:Platinum / Q000737:chemistry*; D014062:Tongue Neoplasms / Q000473:pathology / Q000517:prevention & control*; D014867:Water / Q000737:chemistry / Q000494:pharmacology*
D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D003102:Colloids; D016166:Free Radical Scavengers; D010851:Picrates; D014867:Water; D010984:Platinum; D006859:Hydrogen; C004931:1,1-diphenyl-2-picrylhydrazyl