Failure of the BD GeneOhm StaphSR assay for direct detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates in positive blood cultures collected in the United States.
Fifty-nine Staphylococcus aureus isolates from one hundred blood cultures containing gram-positive cocci in clusters were identified by conventional methods and the BD GeneOhm StaphSR assay (SR). The SR misidentified three methicillin (meticillin)-resistant S. aureus (MRSA) isolates as methicillin-susceptible S. aureus (MSSA), while one MSSA isolate tested negative for S. aureus. The three MRSA isolates were strains with MREJ types that cannot be detected by the currently available SR.
Department of Pathology & Laboratory Medicine, University of Louisville, 530 South Jackson Street, Louisville, KY 40202-8204, USA. jwsnyd01@louisville.edu
James W
JW
Snyder
Gina K
GK
Munier
Stacy A
SA
Heckman
Pamela
P
Camp
Timothy L
TL
Overman
D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D014481:United States
D011933:Reagent Kits, Diagnostic