Effects of phenanthrene on hepatic enzymatic activities in tilapia (Oreochromis niloticus female x O. aureus male).
The effects of phenanthrene (Phe) on hepatosomatic index (HSI) and hepatic enzymatic activities in hybrid tilapia (Oreochromis niloticus female x O. aureus male) were investigated via the static freshwater exposure at dosage of 50, 100, and 400 microg/L for 4-14 d. Compared with the control group, HSI was significantly decreased (P < 0.05) at 400 microg/L at day 14. Increased enzymatic activities (P < 0.05) were observed for catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) at either 100 or 400 microg/L at day 8 and 14, as well as for CAT at 50 microg/L at day 14, except for GPx at 400 microg/L at day 8. Ethoxyresorufin O-deethylase (EROD) activity was significantly increased (P < 0.05) at all dosage at day 4 as well as at 50 microg/L at day 8, but significantly decreased at either 100 or 400 microg/L at day 14 (P < 0.05). Glutathione-S-transferase (GST) activity was not affected. The results suggest that CAT, GPx, SOD and EROD, as well as HSI in tilapia may be used as the biomarkers or indexes for evaluating or monitoring the pollution of polycyclic aromatic hydrocarbons (PAHs) such as Phe.
Marine Biology Institute of Shantou University, Shantou, Guangdong 515063, China. wjxu01@126.com
Wenju
W
Xu
Yuanyou
Y
Li
Qingyang
Q
Wu
Shuqi
S
Wang
Huaiping
H
Zheng
Wenhua
W
Liu
D000818:Animals; D002374:Catalase / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010616:Phenanthrenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D017210:Tilapia / Q000033:anatomy & histology / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
D010616:Phenanthrenes; D014874:Water Pollutants, Chemical; C031181:phenanthrene; D002374:Catalase; D005979:Glutathione Peroxidase; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D005982:Glutathione Transferase