Genetic and functional interactions between the mitochondrial outer membrane proteins Tom6 and Sam37.
The TOM complex is the general mitochondrial entry site for newly synthesized proteins. Precursors of beta-barrel proteins initially follow this common pathway and are then relayed to the SAM/TOB complex, which mediates their integration into the outer membrane. Three proteins, Sam50 (Tob55), Sam35 (Tob38/Tom38), and Sam37 (Mas37), have been identified as the core constituents of the latter complex. Sam37 is essential for growth at elevated temperatures, but the function of the protein is currently unresolved. To identify interacting partners of Sam37 and thus shed light on its function, we screened for multicopy suppressors of sam37Delta. We identified the small subunit of the TOM complex, Tom6, as such a suppressor and found a tight genetic interaction between the two proteins. Overexpression of SAM37 suppresses the growth phenotype of tom6Delta, and cells lacking both genes are not viable. The ability of large amounts of Tom6 to suppress the sam37Delta phenotype can be linked to the capacity of Tom6 to stabilize Tom40, an essential beta-barrel protein which is the central component of the TOM complex. Our results suggest that Sam37 is required for growth at higher temperatures, since it enhances the biogenesis of Tom40, and this requirement can be overruled by improved stability of newly synthesized Tom40 molecules.
Interfaculty Institute for Biochemistry, University of Tübingen, Hoppe-Seyler-Str. 4, 72076 Tübingen, Germany.
Jovana
J
Dukanovic
Kai S
KS
Dimmer
Nathalie
N
Bonnefoy
Katrin
K
Krumpe
Doron
D
Rapaport
D049109:Cell Proliferation; D017353:Gene Deletion; D018628:Gene Dosage; D016153:Genes, Suppressor; D008565:Membrane Proteins / Q000378:metabolism*; D050296:Microbial Viability; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D011498:Protein Precursors / Q000378:metabolism; D055550:Protein Stability; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D008565:Membrane Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins; D011498:Protein Precursors; D021122:Protein Subunits; C092812:SAM37 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C082532:TOM6 protein, S cerevisiae