Accessory and main olfactory systems influences on predator odor-induced behavioral and endocrine stress responses in rats.
Exposures to predator odors are very effective methods to evoke a variety of stress responses in rodents. We have previously found that ferret odor exposure leads to changes in endocrine hormones (corticosterone and ACTH) and behavior. To distinguish the contributions of the main and accessory olfactory systems in these responses, studies were designed to interfere with these two systems either independently, or simultaneously. Male Sprague-Dawley rats were treated with 10% zinc sulfate (ZnSO(4)), which renders rodents anosmic (unable to smell) while leaving the accessory olfactory areas intact, or saline, in Experiment 1. In Experiment 2, the vomeronasal organs of rats were surgically removed (VNX) to block accessory olfactory processing, while leaving the main olfactory system intact. And in the third experiment both the main and accessory olfactory areas were disrupted by combining the two procedures in the same rats. Neither ZnSO(4) treatment nor VNX alone reliably reduced the increased corticosterone response to ferret odor compared to strawberry odor, but in combination, they did. This suggests that processing through the main or the accessory olfactory system can elicit the endocrine stress response to ferret odor. VNX alone also did not affect the behavioral responses to the ferret odor. ZnSO(4) treatment, alone and in combination with VNX, led to changes in behavior in response to both ferret and strawberry odor, making the behavioral results less clearly interpretable. Overall these studies suggest that both the main and accessory olfactory systems mediate the neuroendocrine response to predator odor.
Masini
Cher V
CV
Department of Psychology and Neuroscience & Center for Neuroscience, University of Colorado, Boulder, CO 80309, USA. cher.masini@colorado.edu
Garcia
Robert J
RJ
Sasse
Sarah K
SK
Nyhuis
Tara J
TJ
Day
Heidi E W
HE
Campeau
Serge
S
D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D002452:Cell Count; D003345:Corticosterone / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D004924:Escape Reaction / Q000502:physiology*; D005289:Ferrets; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism / Q000502:physiology; D008297:Male; D009812:Odorants; D000857:Olfaction Disorders / Q000139:chemically induced; D009833:Olfactory Pathways / Q000378:metabolism* / Q000502:physiology; D055696:Olfactory Perception / Q000502:physiology*; D010913:Pituitary-Adrenal System / Q000378:metabolism / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012903:Smell / Q000502:physiology; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology; D019147:Vomeronasal Organ / Q000378:metabolism / Q000502:physiology; D019287:Zinc Sulfate / Q000633:toxicity
D016760:Proto-Oncogene Proteins c-fos; D019287:Zinc Sulfate; D003345:Corticosterone