Concurrent extraction of proteins and RNA from cell-laden hydrogel scaffold free of polysaccharide interference.
Concurrent extractions of proteins and RNA from cell-laden scaffolds would be of great help to biomaterialists and tissue engineers. Here we describe a procedure to extract proteins from the discard solution generated during the RNA isolation from polysaccharide-rich hydrogel scaffolds. This approach allows to obtain proteins and RNA from same sample while eliminating the polysaccharide interference.
Wang
Chunming
C
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, Singapore 637457, Republic of Singapore.
Li
Xin
X
Yao
Yongchang
Y
Wang
Dong-An
DA
D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D011134:Polysaccharides; D011506:Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012313:RNA