Null mutation analysis of an afsA-family gene, barX, that is involved in biosynthesis of the {gamma}-butyrolactone autoregulator in Streptomyces virginiae.
Virginiae butanolide (VB) is a gamma-butyrolactone autoregulator that triggers production of the streptogramin antibiotic virginiamycin in Streptomyces virginiae. Our previous studies suggested that the barX gene, an afsA-family gene, is likely to participate in the regulatory pathway for the production of VB, rather than in the biosynthetic pathway of VB itself, in contrast to the function of other afsA-family genes. Mutation analysis now shows that BarX at least plays an enzymic role in the VB biosynthetic pathway. Heterologous expression of the afsA gene from Streptomyces griseus into the barX mutant partially restored the deficiency of virginiamycin production, suggesting that afsA-family genes have a common ability to synthesize the gamma-butyrolactone autoregulators. Taken together with previous works relating to the function of an afsA-family gene, these results support the idea that streptomycetes have two biosynthetic pathways for the gamma-butyrolactone autoregulators.
Lee
Yong Jik
YJ
International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Kitani
Shigeru
S
International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nihira
Takuya
T
MU-OU Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd, 10400 Bangkok, Thailand.|International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
D015107:4-Butyrolactone / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D012329:RNA, Bacterial / Q000235:genetics; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014769:Virginiamycin / Q000096:biosynthesis
D001426:Bacterial Proteins; C412004:BarX protein, Streptomyces virginiae; D012329:RNA, Bacterial; D014769:Virginiamycin; D015107:4-Butyrolactone