Spectrophotometric and HPLC determinations of anti-diabetic drugs, rosiglitazone maleate and metformin hydrochloride, in pure form and in pharmaceutical preparations.
In this study, three spectrophotometric methods and one HPLC method were developed for analysis of anti-diabetic drugs in tablets. The two spectrophotometric methods were based on the reaction of rosiglitazone (RSG) with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and bromocresol green (BCG). Linear relationship between the absorbance at lambda(max) and the drug concentration was found to be in the ranges 6.0-50.0 and 1.5-12 microg ml(-1) for DDQ and BCG methods, respectively. The third spectrophotometric method consists of a zero-crossing first-derivative spectrophotometric method for simultaneous analysis of RSG and metformin (MTF) in tablets. The calibration curves were linear within the concentration ranges of 5.0-50 microg ml(-1) for RSG and 1.0-10.0 microg ml(-1) for MTF. The fourth method is a rapid stability-indicating HPLC method developed for the determination of RSG. A linear response was observed within the concentration range of 0.25-2.5 microg ml(-1). The proposed methods have been successfully applied to the tablet analysis.
Onal
Armağan
A
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, 34116 Beyazit, Istanbul, Turkey. armaganozkul@yahoo.com
D002626:Chemistry, Pharmaceutical / Q000191:economics; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000627:therapeutic use; D008687:Metformin / Q000737:chemistry* / Q000627:therapeutic use; D015394:Molecular Structure; D000077154:Rosiglitazone; D013053:Spectrophotometry; D013607:Tablets / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry* / Q000627:therapeutic use; D045162:Thiazolidinediones / Q000737:chemistry* / Q000627:therapeutic use
D004338:Drug Combinations; D007004:Hypoglycemic Agents; D013607:Tablets; D013844:Thiazoles; D045162:Thiazolidinediones; C471074:rosiglitazone-metformin combination; D000077154:Rosiglitazone; D008687:Metformin