Concentration method for the detection of enteric viruses from large volumes of foods.
Enteric viruses are the major cause of outbreaks of foodborne viral disease worldwide, and vegetables and fruits are considered significant vectors of virus transmission. In this study, we compared viral elution concentration methods in strawberry and lettuce and tested the secondary concentration step for concentrating viruses from large volumes of lettuce samples. Among the tested procedures, the combination of a 0.05 M glycine plus 100 mM Tris elution buffer (pH 9.5) and a polyethylene glycol precipitation concentration was most efficient for the detection of norovirus genogroup II from strawberries (50% of samples) and lettuce (2.9% of samples). The secondary concentration step using ultrafiltration devices could be applied to large lettuce samples without any decrease in detection limit and efficiency, and other cultivable enteric viruses including enteroviruses, adenoviruses, and rotaviruses were recovered from lettuce at efficiencies of 11.4, 9.05, and 11.3%, respectively. This method could be useful for detecting enteric viruses in fresh foods.
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, South Korea.
Sooryun
S
Cheong
Cheonghoon
C
Lee
Weon Cheon
WC
Choi
Chan-Hee
CH
Lee
Sang-Jong
SJ
Kim
D002478:Cells, Cultured; D011232:Chemical Precipitation; D004770:Enterovirus / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D031985:Fragaria / Q000821:virology*; D006801:Humans; D018545:Lactuca / Q000821:virology*; D016133:Polymerase Chain Reaction; D014461:Ultracentrifugation