A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation.
We report a systematic study of the condensation of plasmid DNA by oligocations with variation of the charge, Z, from +3 to +31. The oligocations include a series of synthetic linear epsilon-oligo(l-lysines), (denoted epsilonKn, n = 3-10, 31; n is the number of lysines equal to the ligand charge) and branched alpha-substituted homologues of epsilonK10: epsilonYK10, epsilonLK10 (Z = +10); epsilonRK10, epsilonYRK10 and epsilonLYRK10 (Z = +20). Data were obtained by light scattering, UV absorption monitored precipitation assay and isothermal titration calorimetry in a wide range concentrations of DNA and monovalent salt (KCl, C(KCl)). The dependence of EC(50) (ligand concentration at the midpoint of DNA condensation) on C(KCl) shows the existence of a salt-independent regime at low C(KCl) and a salt-dependent regime with a steep rise of EC(50) with increase of C(KCl). Increase of the ligand charge shifts the transition from the salt-independent to salt-dependent regime to higher C(KCl). A novel and simple relationship describing the EC(50) dependence on DNA concentration, charge of the ligand and the salt-dependent dissociation constant of the ligand-DNA complex is derived. For the epsilon-oligolysines epsilonK3-epsilonK10, the experimental dependencies of EC(50) on C(KCl) and Z are well-described by an equation with a common set of parameters. Implications from our findings for understanding DNA condensation in chromatin are discussed.
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. korolev@ntu.edu.sg
Nikolay
N
Korolev
Nikolay V
NV
Berezhnoy
Khee Dong
KD
Eom
James P
JP
Tam
Lars
L
Nordenskiöld
D002151:Calorimetry; D002412:Cations / Q000737:chemistry; D011232:Chemical Precipitation; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D008027:Light; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D011107:Polylysine / Q000737:chemistry*; D011189:Potassium Chloride / Q000737:chemistry; D012542:Scattering, Radiation; D055672:Static Electricity
D002412:Cations; D008024:Ligands; D011107:Polylysine; D011189:Potassium Chloride; D004247:DNA