Evaluation of protective immunity of 28KD-GST as a vaccine against experimental Schistosoma mansoni.
Schistosoma mansoni GST was purified from adult worm homogenates by affinity chromatography. SDS-PAGE analysis of the purified antigen revealed that SmGST has molecular weight around 28 KD was used as a vaccine in a dose of 25 and 35 microg. Eleven groups of BALB/c mice of 10 mice each were vaccinated by GST. Complete Freund's adjuvant (CFA) and BCG vaccine were used as adjuvant. Booster doses were given after 2 & 4 weeks. Two weeks after the last dose of vaccine, mice were challenged by S. mansoni cercariae. Blood samples were taken 7 weeks post infection for detection of IgGl, IgE and circulating antigens. Then mice were sacrificed for histopathological study of the liver. Highly significant (p > 0.001) increase in the mean optical density of IgGl & IgE in groups vaccinated by 35 microg GST and CFA was demonstrated. On the other hand, highly significant (P < 0.001) decrease in circulating antigen, grnuloma number and size in the same group.
Department of Parsitology, Faculty of Medicine, Zagazig University, Zagazig, Sharkia Governorate, Egypt.
Mohsen M
MM
Hassan
Nahed E
NE
Moustafa
Mona H
MH
El-Motayam
Magda A
MA
El-Settawy
Afaf A
AA
Taha
D000276:Adjuvants, Immunologic; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005620:Freund's Adjuvant; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D011897:Random Allocation; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000517:prevention & control*
D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D001500:BCG Vaccine; D015801:Helminth Proteins; C052358:P28 antigen, Schistosoma; D016052:Protozoan Vaccines; D005620:Freund's Adjuvant