[Novel methods and strategies for strain improvement].
Improvement of the productivity of industrial strains is an important field in micro-biology, because wild-type strains isolated from nature usually produce only a low level of antibiotics. Although random screening and simple rational screening are still effective without using genomic information, they are always time- and labor-consuming. With the broad application of recombinant DNA technology, protoplast fusion and X-omics, novel methods and strategies such as metabolic engineering, genome shuffling, system biology and system biotechnology, ribosome engineering, epigenetic modification are being exploited for the industry microbiology. In this review, we will focus on the progress of these novel methods and strategies for strain improvement in recent years.
Institute of Modern Biopharmaceuticals, School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Wenting
W
Zhao
Yi
Y
Zou
Changhua
C
Hu
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000639:trends; D032621:DNA Shuffling / Q000379:methods*; D004274:DNA, Recombinant; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D007218:Industrial Microbiology / Q000379:methods*; D013678:Technology, Pharmaceutical
D004274:DNA, Recombinant