Cloning and characterization of voltage-gated calcium channel alpha1 subunits in Xenopus laevis during development.
Voltage-gated calcium channels play a critical role in regulating the Ca2+ activity that mediates many aspects of neural development, including neural induction, neurotransmitter phenotype specification, and neurite outgrowth. Using Xenopus laevis embryos, we describe the spatial and temporal expression patterns during development of the 10 pore-forming alpha1 subunits that define the channels' kinetic properties. In situ hybridization indicates that CaV1.2, CaV2.1, CaV2.2, and CaV3.2 are expressed during neurula stages throughout the neural tube. These, along with CaV1.3 and CaV2.3, beginning at early tail bud stages, and CaV3.1 at late tail bud stages, are detected in complex patterns within the brain and spinal cord through swimming tadpole stages. Additional expression of various alpha1 subunits was observed in the cranial ganglia, retina, olfactory epithelium, pineal gland, and heart. The unique expression patterns for the different alpha1 subunits suggests they are under precise spatial and temporal regulation and are serving specific functions during embryonic development.
Lewis
Brittany B
BB
Department of Biology, The College of William and Mary, Integrated Science Center, Williamsburg, Virginia 23185, USA.
Wester
Matthew R
MR
Miller
Lauren E
LE
Nagarkar
Maitreyi D
MD
Johnson
M Brittany
MB
Saha
Margaret S
MS
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism*; D020864:Calcium Channels, N-Type / Q000235:genetics / Q000378:metabolism*; D020747:Calcium Channels, T-Type / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000196:embryology* / Q000378:metabolism; D006321:Heart / Q000196:embryology; D054261:Neurulation; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism
C000595059:CACNA1A protein, Xenopus; D020746:Calcium Channels, L-Type; D020864:Calcium Channels, N-Type; D020747:Calcium Channels, T-Type; D029867:Xenopus Proteins