Development of a fast capillary electrophoresis method for the determination of propranolol-Total analysis time reduction strategies.
The aim of this study was to develop a fast capillary electrophoresis method for the determination of propranolol in pharmaceutical preparations. In the method development the pH and constituents of the background electrolyte were selected using the effective mobility versus pH curves. Benzylamine was used as the internal standard. The background electrolyte was composed of 60mmolL(-1) tris(hydroxymethyl)aminomethane and 30mmolL(-1) 2-hydroxyisobutyric acid, at pH 8.1. Separation was conducted in a fused-silica capillary (32cm total length and 8.5cm effective length, 50microm I.D.) with a short-end injection configuration and direct UV detection at 214nm. The run time was only 14s. Three different strategies were studied in order to develop a fast CE method with low total analysis time for propranolol analysis: low flush time (Lflush) 35runs/h, without flush (Wflush) 52runs/h, and Invert (switched polarity) 45runs/h. Since the three strategies developed are statistically equivalent, Wflush was selected due to the higher analytical frequency in comparison with the other methods. A few figures of merit of the proposed method include: good linearity (R(2)>0.9999); limit of detection of 0.5mgL(-1); inter-day precision better than 1.03% (n=9) and recovery in the range of 95.1-104.5%.
Department of Chemistry, Federal University of Santa Catarina, 88040-900 Florianopolis, SC, Brazil. gustavomicke@qmc.ufsc.br
Gustavo Amadeu
GA
Micke
Ana Carolina O
AC
Costa
Melina
M
Heller
Michelle
M
Barcellos
Marcel
M
Piovezan
Thiago
T
Caon
Marcone Augusto Leal
MA
de Oliveira
D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011433:Propranolol / Q000032:analysis*; D013997:Time Factors
D000319:Adrenergic beta-Antagonists; D004364:Pharmaceutical Preparations; D011433:Propranolol