APE1/Ref-1 in Alzheimer's disease: an immunohistochemical study.
The oxidative injury in Alzheimer's disease (AD), in which amyloid beta protein induces production of reactive oxygen species, may be cause of neurodegeneration. APE1/Ref-1 is a protein involved in DNA repair and in redox co-activating function over different transcription factors. We investigated by immunohistochemistry using a highly specific monoclonal antibody, the localization of APE1/Ref-1 in autoptic and bioptic AD brain tissues in comparison with brains with unrelated pathological or normal conditions. Reliable APE1/Ref-1 immunostaining was obtained in biopsies, but not in autoptic tissues. An increased nuclear expression of APE1/Ref-1 in AD cerebral cortex supports the view that the cellular adaptive response to the oxidative stress condition is involved in the pathogenesis of this disease.
Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy. gmarcon@istituto-besta.it
Gabriella
G
Marcon
Gianluca
G
Tell
Lorena
L
Perrone
Rita
R
Garbelli
Franco
F
Quadrifoglio
Fabrizio
F
Tagliavini
Giorgio
G
Giaccone
D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D001344:Autopsy; D001706:Biopsy; D001921:Brain / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged
D000911:Antibodies, Monoclonal; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase