Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film.
The bionanocomposite film consisting of glucose oxidase/Pt/functional graphene sheets/chitosan (GOD/Pt/FGS/chitosan) for glucose sensing is described. With the electrocatalytic synergy of FGS and Pt nanoparticles to hydrogen peroxide, a sensitive biosensor with a detection limit of 0.6 microM glucose was achieved. The biosensor also has good reproducibility, long-term stability and negligible interfering signals from ascorbic acid and uric acid comparing with the response to glucose. The large surface area and good electrical conductivity of graphene suggests that graphene is a potential candidate as a sensor material. The hybrid nanocomposite glucose sensor provides new opportunity for clinical diagnosis and point-of-care applications.
Wu
Hong
H
Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Wang
Jun
J
Kang
Xinhuang
X
Wang
Chongmin
C
Wang
Donghai
D
Liu
Jun
J
Aksay
Ilhan A
IA
Lin
Yuehe
Y
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry
D004800:Enzymes, Immobilized; D010984:Platinum; D006108:Graphite; D048271:Chitosan; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose