Preparation and characterization of a polymeric (PLGA) nanoparticulate drug delivery system with simultaneous incorporation of chemotherapeutic and thermo-optical agents.
The objective of this study was to develop biodegradable poly(DL-lactide-co-glycolic acid) (PLGA) nanoparticles simultaneously loaded with indocyanine green (ICG) and doxorubicin (DOX). The modified oil in water single emulsion solvent evaporation method was used. To enhance the incorporation of both agents and control particle size, four independent processing parameters including amount of polymer, initial ICG content, initial DOX content, and concentration of poly-vinyl alcohol (PVA) were investigated. The ICG and DOX entrapment in nanoparticles as well as the nanoparticle size were determined. The nanoparticles produced by standardized formulation were in the range of 171+/-2 nm (n=3) with low polydispersity index (0.040+/-0.014, n=3). The entrapment efficiency was determined by spectrofluorometer measurements. The efficiency was 44.4+/-1.6% for ICG and 74.3+/-1.9% for DOX. Drug loading was 0.015+/-0.001%, w/w, for ICG and 0.022+/-0.001%, w/w, for DOX (n=3). The release pattern was biphasic. ICG and DOX loaded-nanoparticle preparation was standardized based on the following parameters: PLGA concentration, PVA concentration and initial drug content.
Manchanda
Romila
R
Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA. rmanchan@fiu.edu
Fernandez-Fernandez
Alicia
A
Nagesetti
Abhignyan
A
McGoron
Anthony J
AJ
D002626:Chemistry, Pharmaceutical; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D007208:Indocyanine Green / Q000737:chemistry*; D007700:Kinetics; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D008027:Light; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055620:Optical Phenomena; D010316:Particle Size; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis / Q000737:chemistry; D012015:Reference Standards; D012542:Scattering, Radiation; D013696:Temperature
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004317:Doxorubicin; D011142:Polyvinyl Alcohol; D007208:Indocyanine Green