Dopamine transporter (DAT1) VNTR polymorphism in 12 Indian populations.
The dopamine transporter (DAT1) is a membrane spanning protein that binds the neurotransmitter dopamine and performs re-uptake of dopamine from the synapse into a neuron. The gene encoding DAT1 consists of 15 exons spanning 60 kb on chromosome 5p15.32. Several studies have investigated the possible associations between variants in DAT1 gene and psychiatric disorders. The present study aimed to determine the distribution of the variable number of tandem repeat (VNTR) polymorphism in the 3' untranslated region of DAT1 in 12 Indian populations. A total of 471 healthy unrelated individuals in 12 Indian populations from 3 linguistic groups were included in the present study. The analysis was carried out using PCR and electrophoresis. Overall, 4 alleles of the DAT1 40-bp VNTR, ranging from 7 to 11 repeats were detected. Heterozygosity indices were low and varied from 0.114 to 0.406. The results demonstrate the variability of the DAT1 40-bp VNTR polymorphism in Indian populations and revealed a high similarity with East Asian populations.
Bhaskar
L V K S
LV
Centre for Cellular and Molecular Biology, Hyderabad, India.
Thangaraj
Kumarasamy
K
Mulligan
Connie J
CJ
Wasnik
Samiksha
S
Nandan
Amrita
A
Sharma
Varun Kumar
VK
Sharma
Vishwas
V
Reddy
Alla Govardhana
AG
Singh
Lalji
L
Rao
Vadlamudi Raghavendra
VR
D020413:3' Untranslated Regions; D000483:Alleles; D000704:Analysis of Variance; D001483:Base Sequence; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics*; D005006:Ethnicity; D005787:Gene Frequency; D005843:Geography; D006579:Heterozygote; D006801:Humans; D007194:India; D008037:Linguistics; D018598:Minisatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods
D020413:3' Untranslated Regions; D050483:Dopamine Plasma Membrane Transport Proteins; C493214:SLC6A3 protein, human