Comparison of chromosome preparation methods for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis.
Here we present a comparison of the different chromosome preparation methods, including the method of the single embryo or larva, the method of juvenile-swimming and the method of phytohemagglutinin injection, used for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis. The mean index of mitosis for the three methods was 0.79%, 0.09% and 0.15%, respectively. From the well-spread metaphase chromosomes obtained, it was determined that C. semilaevis has a diploid chromosome number of 42 and heterotypic sex chromosomes. Also, the metaphase chromosomes produced could be used for karyotype preparation and banding studies. The three methods are also successfully used for chromosome preparation in Japanese flounder (Paralichthys olivaceus) and summer flounder (Paralichthys dentatus).
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China.
Chang-Wei
CW
Shao
Peng-Fei
PF
Wu
Xian-Li
XL
Wang
Yong-Sheng
YS
Tian
Song-Lin
SL
Chen
D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes; D020732:Cytogenetic Analysis / Q000379:methods*; D004171:Diploidy; D005412:Flatfishes / Q000235:genetics*; D007621:Karyotyping; D008677:Metaphase; D008938:Mitosis