APE1/Ref-1 in Alzheimer's disease: an immunohistochemical study.
The oxidative injury in Alzheimer's disease (AD), in which amyloid beta protein induces production of reactive oxygen species, may be cause of neurodegeneration. APE1/Ref-1 is a protein involved in DNA repair and in redox co-activating function over different transcription factors. We investigated by immunohistochemistry using a highly specific monoclonal antibody, the localization of APE1/Ref-1 in autoptic and bioptic AD brain tissues in comparison with brains with unrelated pathological or normal conditions. Reliable APE1/Ref-1 immunostaining was obtained in biopsies, but not in autoptic tissues. An increased nuclear expression of APE1/Ref-1 in AD cerebral cortex supports the view that the cellular adaptive response to the oxidative stress condition is involved in the pathogenesis of this disease.
Marcon
Gabriella
G
Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy. gmarcon@istituto-besta.it
Tell
Gianluca
G
Perrone
Lorena
L
Garbelli
Rita
R
Quadrifoglio
Franco
F
Tagliavini
Fabrizio
F
Giaccone
Giorgio
G
D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D001344:Autopsy; D001706:Biopsy; D001921:Brain / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged
D000911:Antibodies, Monoclonal; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase