[The interaction between secreted frizzled-related protein 2 and osteoblast-specific factor 2 in keloid].
OBJECTIVE
To verify the interaction between secreted frizzled-related protein 2 (SFRP2) and osteoblast-specific factor 2 (OSF-2).


METHODS
HA-tagged OSF-2 fusion protein recombinant vector pCMV-HA-OSF-2, which could express in mammal cells was constructed, then identified by enzyme-cutting and transfected into human kidney 293 (HK293) cells with or without Myc-SFRP2 recombinant eukaryotic expression vector pCMV-HA-SFRP2. The interaction between SFRP2 and OSF-2 was detected through coimmunoprecipitation and Western blotting.


RESULTS
In electrophoresis bath, target fragment of SFRP2 coding gene with 800 bp and target gene OSF-2 with 2500 bp could be seen respectively after enzyme-cutting, which showed that pCMV-Myc-SFRP2 and pCMV-HA-OSF-2 were constructed successfully. No HA-OSF-2 expression was detected after pCMV-Myc-SFRP2 or pCMV-HA-OSF-2 transfection. Whereas, HA-OSF-2 expressed by Myc antibody immunoprecipitation after pCMV-Myc-SFRP2 and pCMV-HA-OSF-2 co-transfection.


CONCLUSIONS
HA-OSF-2 recombinant vector can express in mammal cells. Interaction exists between HA-OSF-2 and SFRP2.
Department of Plastic Surgery, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
Zhen-Xiang
ZX
Wang
Xi
X
Tao
Shi-Rong
SR
Li
D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D007627:Keloid / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D000094742:Secreted Frizzled-Related Proteins
D050659:Core Binding Factor Alpha 1 Subunit; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C109618:SFRP2 protein, human; D000094742:Secreted Frizzled-Related Proteins