Multiple proteins and phosphorylations regulate Saccharomyces cerevisiae Cdc24p localization.
Targeting of Saccharomyces cerevisiae Cdc24p to polarized growth sites is essential for its function. Localization of GFP-tagged Cdc24 proteins or fragments was assayed in deletion mutants of Cdc24p-interacting proteins. The boi2Delta, ent2Delta, and hua1Delta mutants showed localization defects. The tos2Delta skg6Delta double mutant displayed aberrant pre-anaphase localization to the mother-bud neck region. The same aberrant pattern was seen when potential phosphorylation sites Ser697, Thr704, and Tyr200 were mutated. The S697A mutation also resulted in phosphorylation defects in vivo. These data support roles for Boi2p, Ent2p, Hua1p, Tos2p, and for Cdc24p phosphorylation in targeting Cdc24p to growth sites.
Department of Microbiology and Molecular Genetics, University of Vermont, 202 Stafford Hall, 95 Carrigan Drive, VT 05405, United States.
Karen C
KC
Cole
Joy-El R
JE
Barbour
John F
JF
Midkiff
Brittany M
BM
Marble
Douglas I
DI
Johnson
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010766:Phosphorylation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; C099887:BOI2 protein, S cerevisiae; C073594:CDC24 protein, S cerevisiae; D018797:Cell Cycle Proteins; C121321:ENT2 protein, S cerevisiae; D020662:Guanine Nucleotide Exchange Factors; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins