Dual roles for hepatic lectin receptors in the clearance of chilled platelets.
Rapid chilling causes glycoprotein-Ib (GPIb) receptors to cluster on blood platelets. Hepatic macrophage beta(2) integrin binding to beta-N-acetylglucosamine (beta-GlcNAc) residues in the clusters leads to rapid clearance of acutely chilled platelets after transfusion. Although capping the beta-GlcNAc moieties by galactosylation prevents clearance of short-term-cooled platelets, this strategy is ineffective after prolonged refrigeration. We report here that prolonged refrigeration increased the density and concentration of exposed galactose residues on platelets such that hepatocytes, through Ashwell-Morell receptor binding, become increasingly involved in platelet removal. Macrophages rapidly removed a large fraction of transfused platelets independent of their storage conditions. With prolonged platelet chilling, hepatocyte-dependent clearance further diminishes platelet recovery and survival after transfusion. Inhibition of chilled platelet clearance by both beta(2) integrin and Ashwell-Morell receptors may afford a potentially simple method for storing platelets in the cold.
Rumjantseva
Viktoria
V
Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
Grewal
Prabhjit K
PK
Wandall
Hans H
HH
Josefsson
Emma C
EC
Sørensen
Anne Louise
AL
Larson
Göran
G
Marth
Jamey D
JD
Hartwig
John H
JH
Hoffmeister
Karin M
KM
D000117:Acetylglucosamine / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000378:metabolism*; D001212:Asialoglycoproteins / Q000494:pharmacology; D001781:Blood Component Removal; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods; D001803:Blood Transfusion / Q000379:methods; D018821:CD18 Antigens / Q000378:metabolism; D002236:Carbohydrate Conformation; D002461:Cell Line, Transformed; D003080:Cold Temperature; D005434:Flow Cytometry; D005690:Galactose / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology; D010586:Phagocytes / Q000187:drug effects; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D012034:Refrigeration / Q000379:methods; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000494:pharmacology
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D018821:CD18 Antigens; D010446:Peptide Fragments; D019038:Platelet Glycoprotein GPIb-IX Complex; D000509:alpha-Fetoproteins; C085598:asialofetuin 12 peptide; C032193:glycoprotein receptor GPIb-IX; D000117:Acetylglucosamine; D005690:Galactose