Molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap-3) by a new class-II piggyBac-related insect transposon.
A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.
Carpes
M P
MP
Laboratório de Microscopia Eletrônica, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
Nunes
J F
JF
Sampaio
T L
TL
Castro
M E B
ME
Zanotto
P M A
PM
Ribeiro
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics* / Q000502:physiology*; D004587:Electrophoresis, Agar Gel; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D009154:Mutation / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D004251:DNA Transposable Elements; D051034:Inhibitor of Apoptosis Proteins