Knockdown of angiotensinogen by shRNA-mediated RNA interference inhibits human visceral preadipocytes differentiation.
OBJECTIVE
The immediate cause of obesity is the massive deposition of subcutaneous and visceral fat attributing to the continuous proliferation and differentiation of preadipocytes. The identification of the underlying molecular mechanisms of preadipocytes differentiation is urgent, and will have an important role in plastic and reconstructive surgical procedures.


METHODS
Two small hairpin RNA (shRNA)-mediated RNA interference plasmids have been constructed on the basis of the activity of H1 promoter-driven expression vector psiRNA-hH1neo to suppress the expression of angiotensinogen (AGT) in human preadipocytes-visceral (HPA-v). Subsequently, glycerol-3-phosphate dehydrogenase (G3PDH) activity and intracytoplasmic lipids content were detected during the process of HPA-v differentiation.


RESULTS
Small hairpin RNA-expressing vectors have been successfully constructed to suppress the expression of AGT significantly. Both intracytoplasmic lipids content and G3PDH activity decreased to a certain extent compared with that in the control group in the whole process of HPA-v differentiation.


CONCLUSIONS
Two shRNA-mediated AGT-targeting plasmids inhibited the process of HPA-v differentiation to a certain extent. However, the accumulation of intracytoplasmic lipids was not exclusively determined by the expression of AGT, and it may also be regulated by other factors. In conclusion, this study provided a method to inhibit the process of preadipocytes differentiation, and it may have a role in obesity treatment and adipose tissue engineering application.
Ye
Z-W
ZW
College of Food and Bioengineering, South China University of Technology, Guangzhou, China.
Wu
X-M
XM
Zhang
Li-Jun
LJ
Huang
Zhi-Li
ZL
Jiang
J-G
JG
D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D000808:Angiotensinogen / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D055029:Inverted Repeat Sequences; D050356:Lipid Metabolism / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics
D034741:RNA, Small Interfering; D000808:Angiotensinogen; D005993:Glycerolphosphate Dehydrogenase