Coating of nitrocellulose for colorimetric DNA microarrays.
We report on the modification of a nitrocellulose film with copoly(DMA-NAS-MAPS), a tercopolymer based on N,N-dimethylacrylamide (DMA), N-acryloyloxysuccinimide (NAS), and 3-(trimethoxysilyl)propyl-methacrylate (MAPS). The chains of this polymer, interacting with nitrocellulose fibers, introduce active ester functionalities that promote the covalent binding of short oligonucleotide fragments to the nitrocellulose thin film. Using colorimetric detection, naked eye visible DNA microarrays are developed for easy identification of foodborne pathogens. The fast and robust procedure of nitrocellulose functionalization opens the opportunity to implement this material in disposable analytical microdevices that do not require sophisticated readout systems.
Istituto di Chimica del Riconoscimento Molecolare, CNR, 20131 Milano, Italy. marina.cretich@icrm.cnr.it
Marina
M
Cretich
Valentina
V
Sedini
Francesco
F
Damin
Maria
M
Pelliccia
Laura
L
Sola
Marcella
M
Chiari
D000178:Acrylamides / Q000737:chemistry; D003101:Collodion / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005517:Foodborne Diseases / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017646:Organosilicon Compounds / Q000737:chemistry; D016133:Polymerase Chain Reaction
D000178:Acrylamides; D004269:DNA, Bacterial; D008689:Methacrylates; D017646:Organosilicon Compounds; D003101:Collodion; C099046:N,N-dimethylacrylamide; C505504:3-(trimethoxysilyl)propyl methacrylate