Quantum dots for labeling adipose tissue-derived stem cells.
Adipose tissue-derived stem cells (ASCs) have a self-renewing ability and can be induced to differentiate into various types of mesenchymal tissue. Because of their potential for clinical application, it has become desirable to label the cells for tracing transplanted cells and for in vivo imaging. Quantum dots (QDs) are novel inorganic probes that consist of CdSe/ZnS-core/shell semiconductor nanocrystals and have recently been explored as fluorescent probes for stem cell labeling. In this study, negatively charged QDs655 were applied for ASCs labeling, with the cationic liposome, Lipofectamine. The cytotoxicity of QDs655-Lipofectamine was assessed for ASCs. Although some cytotoxicity was observed in ASCs transfected with more than 2.0 nM of QDs655, none was observed with less than 0.8 nM. To evaluate the time dependency, the fluorescent intensity with QDs655 was observed until 24 h after transfection. The fluorescent intensity gradually increased until 2 h at the concentrations of 0.2 and 0.4 nM, while the intensity increased until 4 h at 0.8 nM. The ASCs were differentiated into both adipogenic and osteogenic cells with red fluorescence after transfection with QDs655, thus suggesting that the cells retain their potential for differentiation even after transfected with QDs655. These data suggest that QDs could be utilized for the labeling of ASCs.
Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461-0047, Japan. hiroshiy@med.nagoya-u.ac.jp
Hiroshi
H
Yukawa
Shogo
S
Mizufune
Chiharu
C
Mamori
Yukimasa
Y
Kagami
Koichi
K
Oishi
Noritada
N
Kaji
Yukihiro
Y
Okamoto
Manabu
M
Takeshi
Hirofumi
H
Noguchi
Yoshinobu
Y
Baba
Michinari
M
Hamaguchi
Nobuyuki
N
Hamajima
Shuji
S
Hayashi
D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis; D045663:Quantum Dots; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors
D005456:Fluorescent Dyes; D008081:Liposomes