Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings.
In order to create a moist environment for rapid wound healing, a hydrogel sheet composed of a blended powder of alginate, chitin/chitosan and fucoidan (ACF-HS; 60:20:2:4 w/w) has been developed as a functional wound dressing. ACF-HS gradually absorbed DMEM without any maceration, and fluid absorption became constant within 18 h. On application, ACF-HS was expected to effectively interact with and protect the wound in rats, providing a good moist healing environment with exudates. In addition, the wound dressing has properties such as ease of application and removal and good adherence. Full-thickness skin defects were made on the backs of rats and mitomycin C solution (1 mg/ml in saline) was applied onto the wound for 10 min in order to prepare healing-impaired wounds. After thoroughly washing out the mitomycin C, ACF-HS was applied to the healing-impaired wounds. Although normal rat wound repair was not stimulated by the application of ACF-HS, healing-impaired wound repair was significantly stimulated. Histological examination demonstrated significantly advanced granulation tissue and capillary formation in the healing-impaired wounds treated with ACF-HS on day 7, as compared to those treated with calcium alginate fiber (Kaltostat; Convatec Ltd., Tokyo, Japan) and those left untreated.
Department of Oral and Maxillofacial Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Kaoru
K
Murakami
Hiroshi
H
Aoki
Shingo
S
Nakamura
Shin-ichiro
S
Nakamura
Megumi
M
Takikawa
Motoaki
M
Hanzawa
Satoko
S
Kishimoto
Hidemi
H
Hattori
Yoshihiro
Y
Tanaka
Tomoharu
T
Kiyosawa
Yasunori
Y
Sato
Masayuki
M
Ishihara
D000464:Alginates; D000818:Animals; D001458:Bandages; D002478:Cells, Cultured; D002686:Chitin; D048271:Chitosan; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D020100:Hydrogels; D016685:Mitomycin / Q000008:administration & dosage; D011134:Polysaccharides; D051381:Rats; D014945:Wound Healing
D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D011134:Polysaccharides; D002686:Chitin; D016685:Mitomycin; D020723:Glucuronic Acid; D048271:Chitosan; C007789:fucoidan