Analysis of protein conformation and dynamics by hydrogen/deuterium exchange MS.
Understanding as much as possible about proteins in the shortest amount of time has long been a goal of hydrogen exchange (HX) MS. Recent technological advances have led to improvements in the technique, but has this goal yet been achieved? (To listen to a podcast about this Feature, please go to the Analytical Chemistry Web site at pubs.acs.org/journal/ancham.).
Northeastern University, Boston, MA 02115-5000, USA. j.engen@neu.edu
John R
JR
Engen
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D003903:Deuterium / Q000737:chemistry*; D041961:Deuterium Exchange Measurement / Q000379:methods*; D006859:Hydrogen / Q000737:chemistry*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D006859:Hydrogen; D003903:Deuterium