MCE enzyme immunoassay for carcinoembryonic antigen and alpha-fetoprotein using electrochemical detection.
An MCE electrochemical enzyme immunoassay protocol for the determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was reported. Two antigens (Ag), CEA and AFP, were incubated simultaneously with an excess amount of horseradish peroxidase-labeled antibody (Ab(*)). The free Ab(*) and the Ab(*)-Ag complex produced in the solution were first separated through a postcolumn reaction and then traced by the enzyme substrate o-aminophenol. The 3-aminophenoxazine produced in enzyme reaction was detected with downstream amperometric detection. The separations were performed at a separation voltage of +1.4 kV and were completed in less than 60 s. The better analytical performance and distinct miniaturization/portability for MCE at less assay time and sample volume consumption was achieved. The detection limit of CEA and AFP was calculated to be 0.25 and 0.13 ng/mL, respectively. Therefore, MCE could be used as a sensitive and new tool in separation science and offered considerable promise in biological sample analysis or quick clinical diagnosis.
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, P. R. China. shushzhang@126.com
Shusheng
S
Zhang
Wei
W
Cao
Jing
J
Li
Mingming
M
Su
D002272:Carcinoembryonic Antigen / Q000097:blood* / Q000276:immunology; D004563:Electrochemistry / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D007124:Immunoenzyme Techniques / Q000191:economics / Q000379:methods*; D057230:Limit of Detection; D044967:Serum / Q000737:chemistry; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D002272:Carcinoembryonic Antigen; D000509:alpha-Fetoproteins