Deproteinized bone with VEGF gene transfer to facilitate the repair of early avascular necrosis of femoral head of rabbit.
OBJECTIVE
To explore a new method for early avascular necrosis of femoral head (AVNFH) therapy.


METHODS
Sixty-nine AVNFH New Zealand adult rabbits were randomly divided into three groups with equal number. In Group A, deproteinized bone (DPB) that absorbed with recombinant plasmid pcDNA3.1-hVEGF165 was implanted into the drilled tunnel of necrotic femoral head. In Group B, only DPB was implanted. In Group C, only tunnel was drilled without DPB or plasmid implanted. Femoral head specimens were obtained at postoperative 1, 2, 4, 8, 16 weeks. The expression of VEGF165 and collagen I was detected by immunohistochemistry. Bone formation was detected generally by X-ray. Angiogenesis and the repair of the femoral head were observed histologically.


RESULTS
The expression of VEGF 165 could be detected 2 weeks after implantation in Group A, but it was not observed in other groups. The result of collagen I expression had a significantly difference 2, 4 and 8 weeks after operation in Group A from those in other groups (P < 0.01). X-ray results indicated that there was more bone formation in Group A than in other groups. The regenerated capillary vessels staining result of necrotic femoral head in Group A was significantly different from those in other groups at postoperative 2 and 4 weeks (P < 0.01).


CONCLUSIONS
Transfection of hVEGF165 gene enhances local angiogenesis and DPB-VEGF compound improves the repair of necrotic femoral head. Deproteinized bone grafting with VEGF gene transfer provides a potential method for the treatment of osteonecrosis.
Department of Orthopedics, First Affiliated Hospital, Nanchang University, Nanchang 330006, China. osteo.caokai@yahoo.com.cn
Kai
K
Cao
Wei
W
Huang
Hong
H
An
Dian-ming
DM
Jiang
Yong
Y
Shu
Zhi-min
ZM
Han
D000818:Animals; D016025:Bone Transplantation; D024042:Collagen Type I / Q000032:analysis; D005271:Femur Head Necrosis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D015316:Genetic Therapy; D007150:Immunohistochemistry; D018919:Neovascularization, Physiologic; D011817:Rabbits; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000235:genetics*
D024042:Collagen Type I; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A