Antisecretory activity from the flowers of Chiranthodendron pentadactylon and its flavonoids on intestinal fluid accumulation induced by Vibrio cholerae toxin in rats.
BACKGROUND
The flowers of Chiranthodendron pentadactylon Larreat. (Sterculiaceae) has been traditionally used as folk medicine in Mexico for the treatment of gastrointestinal disorders such as diarrhea and dysentery.


OBJECTIVE
This study aimed to assess the antisecretory activity which supports the therapeutic use of Chiranthodendron pentadactylon and its flavonoids to treat diarrhea.


METHODS
The methanol extract of Chiranthodendron pentadactylon, subsequent fractions, and flavonoids were evaluated on cholera toxin-induced intestinal secretion in rat jejunal loops model.


RESULTS
Three antisecretory flavonoids were isolated by bioassay-guided purification, namely, isoquercitrin 3, (+)-catechin 4 and (-)-epicatechin 5. Among them, epicatechin exhibited the most potent antisecretory activity with ID(50) of 8.3 microM/kg. Its potency was close that of to loperamide (ID(50) 6.1 microM/kg), drug used as control. Isoquercitrin (ID(50) 19.2 microM/kg) and catechin (ID(50) 51.7 microM/kg) showed moderate and weak activity, respectively.


CONCLUSIONS
The results of the present study lend some support to the anecdotal report for the traditional use of the flowers of Chiranthodendron pentadactylon in the control of dysentery.
Unidad de Investigación Médica en Farmacología de Productos Naturales, Hospital de Pediatría, CORCE 2 piso Centro Médico Nacional Siglo XXI, IMSS, Av. Cuauhtémoc 330, Col. Doctores, CP 06725, México, DF, Mexico. cvg09@yahoo.com
Claudia
C
Velázquez
Fernando
F
Calzada
Baldomero
B
Esquivel
Elizabeth
E
Barbosa
Samuel
S
Calzada
D000818:Animals; D001826:Body Fluids; D002772:Cholera Toxin / Q000633:toxicity*; D002852:Chromatography, Ion Exchange; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D008297:Male; D019660:Malvaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005419:Flavonoids; D010936:Plant Extracts; D002772:Cholera Toxin