The influence of feeding and fasting on plasma metabolites in the dogfish shark (Squalus acanthias).
Dogfish sharks are opportunistic predators, eating large meals at irregular intervals. Here we present a synthesis of data from several previous studies on responses in plasma metabolites after natural feeding and during prolonged fasting (up to 56days), together with new data on changes in plasma concentrations of amino acids and non-esterified fatty acids. Post-prandial and long-term fasting responses were compared to control sharks fasted for 7days, a typical inter-meal interval. A feeding frenzy was created in which dogfish were allowed to feed naturally on dead teleosts at two consumed ration levels, 2.6% and 5.5% of body weight. Most responses were more pronounced at the higher ration level. These included increases in urea and TMAO concentrations at 20h, followed by stability through to 56days of fasting. Ammonia levels were low and exhibited little short-term response to feeding, but declined to very low values during the extended fast. Glucose and beta-hydroxybutyrate both fell after feeding, the latter to a greater and more prolonged extent (up to 60h), whereas acetoacetate did not change. During prolonged fasting, glucose concentrations were well regulated, but beta-hydroxybutyrate increased to 2-3-fold control levels. Total plasma amino acid concentrations increased in a biphasic fashion, with peaks at 6-20h, and 48-60h after the meal, followed by homeostasis during the extended fast. Essential and non-essential amino acids generally followed this same pattern, though some exhibited different trends after feeding: taurine, beta-alanine, and glycine (decreases or stability), alanine and glutamine (modest prolonged increases), and threonine, serine, asparagine, and valine (much larger short-term increases). Plasma non-esterified fatty acid concentrations declined markedly through 48h after the 2.6% meal. These data are interpreted in light of companion studies showing elevations in aerobic metabolic rate, urea production, rectal gland function, metabolic base excretion, and activation of ornithine-urea cycle and aerobic enzymes after the meal, and muscle N-depletion but maintenance of osmolality and urea production during long-term fasting.
Wood
Chris M
CM
Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada. woodcm@mcmaster.ca <woodcm@mcmaster.ca>
Walsh
Patrick J
PJ
Kajimura
Makiko
M
McClelland
Grant B
GB
Chew
Shit F
SF
D020155:3-Hydroxybutyric Acid / Q000097:blood; D000596:Amino Acids / Q000097:blood*; D000641:Ammonia / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004284:Dogfish / Q000097:blood / Q000378:metabolism*; D004435:Eating; D004734:Energy Metabolism; D005215:Fasting / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005247:Feeding Behavior; D008744:Methylamines / Q000097:blood; D009994:Osmolar Concentration; D019518:Postprandial Period; D011235:Predatory Behavior; D013997:Time Factors; D014508:Urea / Q000097:blood
D000596:Amino Acids; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D008744:Methylamines; D000641:Ammonia; D014508:Urea; C005855:trimethyloxamine; D020155:3-Hydroxybutyric Acid