Unstable propoxyphene metabolite excreted in human urine is detected by liquid chromatography-tandem mass spectrometry.
A "dilute and shoot" method for measuring norpropoxyphene in human urine using liquid chromatography-tandem mass spectrometry distinguishes two different metabolites of propoxyphene, norpropoxyphene (m/z 326) and a dehydrated rearrangement product (m/z 308). The metabolite formed from the rearrangement and dehydration of norpropoxyphene is excreted in human urine and also may be formed from the chemical degradation of norpropoxyphene. Previously, these two metabolites were indistinguishable by gas chromatography- mass spectrometry methods that use an alkaline extraction that converts norpropoxyphene into its rearrangement product. The degradation of norpropoxyphene presents a challenge for its analytical quantitation, and methods for circumventing these issues are presented.
Millennium Laboratories, 16981 Via Tazon, San Diego, California 92117, USA. bcrews@becausepainmatters.com
Bridgit
B
Crews
Charles
C
Mikel
Sergey
S
Latyshev
Robert
R
West
Amadeo
A
Pesce
D000701:Analgesics, Opioid / Q000652:urine*; D001711:Biotransformation; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D011431:Dextropropoxyphene / Q000031:analogs & derivatives / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D012015:Reference Standards; D012996:Solutions; D053719:Tandem Mass Spectrometry
D000701:Analgesics, Opioid; D007202:Indicators and Reagents; D012996:Solutions; C002756:norpropoxyphene; D011431:Dextropropoxyphene