Transient assays for the analysis of miRNA processing and function.
Transient assays provide a convenient alternative to stable transformation. For small RNA analysis in plants, the most widely used method, commonly named agroinfiltration, makes use of Agrobacterium tumefaciens to deliver transgenes into leaf cells of Nicotiana benthamiana. Compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. Agroinfiltration has been used successfully in many different applications, including the analysis of small RNAs. We describe here a protocol for analysis of miRNA processing using agroinfiltration of N. benthamiana leaves.
de Felippes
Felipe F
FF
Max Planck Institute for Developmental Biology, Tübingen, Germany.
Weigel
Detlef
D
D016960:Agrobacterium tumefaciens / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D014026:Nicotiana / Q000235:genetics
D035683:MicroRNAs