Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles.
The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs-KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20-800 nmol L(-1) and 2 nmol L(-1), respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94-104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs-KANA system, may provide a new approach for determination of compounds rich in OH groups.
Wang
Xinyi
X
The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Zou
Mingjian
M
Xu
Xiao
X
Lei
Rong
R
Li
Kean
K
Li
Na
N
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000652:urine; D006046:Gold; D006801:Humans; D007612:Kanamycin / Q000737:chemistry / Q000652:urine*; D053768:Metal Nanoparticles; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D014508:Urea
D000900:Anti-Bacterial Agents; D007612:Kanamycin; D006046:Gold; D014508:Urea