[Development of a comprehensive approach to detecting the antituberculous activity of low molecular-weight chemical compounds].
The optimal parameters of the Alamar Blue test have been determined to detect the antituberculous activity of the chemical compounds under study. The duration of mycobacterial cell incubation before addition of Alamar Blue is 24 hours; that is 17 hours for both H37Ra and H37Rv M. tuberculosis. A method has been devised to evaluate the bactericidal/bacteriostatic activity of the chemical compounds. A thoroughly characterized collection of clinical M. tuberculosis strains that differ in drug sensitivity has been created. A procedure has been developed to reveal the activity of the chemical compounds, by applying mono and multiresistant M. tuberculosis strains. Variability in the growth rate for the clinical strains of mycobacterial cultures is shown. A method has been devised to evaluate the toxicity of the chemical compounds for eukaryotic cells.
V N
VN
Stepanshina
T N
TN
Mukhina
S A
SA
Blagodatskikh
R I
RI
Mironova
I G
IG
Shemiakin
E V
EV
Belova
D000995:Antitubercular Agents / Q000494:pharmacology*; D004339:Drug Compounding; D015195:Drug Design; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D014376:Tuberculosis / Q000188:drug therapy* / Q000382:microbiology
D000995:Antitubercular Agents