Characterisation of N-glycans bound to IGFBP-3 in sera from healthy adults.
Human IGFBP-3 contains three potential N-linked glycosylation sites. Published data concerning the type and saccharide composition of the N-glycans is scarce. The aim of this study was to characterise N-glycans covalently attached to IGFBP-3 from sera of healthy adults (men and women). In order to do that a panel of eight lectins covering broad saccharide specificity was used: agarose-immobilised SNA (Sambucus nigra agglutinin), Con A (lectin from Canavalia ensiformis), RCA I (Ricinus communis agglutinin I), PHA-E (Phaseolus vulgaris erythroagglutinin), PHA-L (P. vulgaris leukoagglutinin), succinylated WGA (wheat germ agglutinin), ECL (Erythrina cristagalli lectin) and UEA (Ulex europaeus agglutinin). IGFBP-3 interacted with SNA, Con A, RCA I, PHA-E and, to a much lesser extent, with PHA-L. These results indicate that human IGFBP-3 bears mostly biantennary complex type N-glycans with a very high content of alpha-2,6-linked Sia at their termini. Hybrid type and high-mannose type N-glycans are present, as well as a bisecting GlcNAc residue, which may be core fucosylated. N-glycosylation of IGFBP-3 follows the N-glycosylation pattern of major serum proteins. This study represents a ground for the future research of glycosylation pattern of IGFBP-3 from the circulation of men and women diagnosed with different illnesses.
Masnikosa
Romana
R
INEP-Institute for the Application of Nuclear Energy, University of Belgrade, Serbia. romana@inep.co.rs
Baricević
Ivona
I
Lagundzin
Dragana
D
Nedić
Olgica
O
D000328:Adult; D015153:Blotting, Western; D002846:Chromatography, Affinity; D005260:Female; D006031:Glycosylation; D006262:Health; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood* / Q000378:metabolism*; D008297:Male; D009584:Nitrogen; D037121:Plant Lectins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013379:Substrate Specificity
D018972:Insulin-Like Growth Factor Binding Protein 3; D037121:Plant Lectins; D011134:Polysaccharides; D009584:Nitrogen