Cytologic examination of hemorrhagic fluid by capillary centrifugation: a new technique.
OBJECTIVE
To develop a simplified technique for processing hemorrhagic body fluids, allowing elimination of red blood cells (RBCs) to obtain tumor cell-rich cytosmears for accurate diagnosis of malignancy.


METHODS
Hemorrhagic fluid is collected with ethylenediamine tetraacetic acid anticoagulant. The cells are separated by centrifugation in glass capillaries into 3 layers, with the uppermost containing supernatant, middle buffy coat and lowermost dark layer of RBCs. The smears of the buffy coat are prepared by breaking the capillaries at the junction of buffy coat and RBC layer. The procedure is named capillary centrifugation technique. This procedure was developed in the cytology laboratory at King George's Medical College, Lucknow, India in 1974.


RESULTS
Cell yield is good and cellular details are well preserved. No cell debris is present on the slides. The background of the smears is absolutely clear. Any number of slides may be prepared for special study for exact typing of tumor cells


CONCLUSIONS
This is a simple, economical procedure and a good substitute for a cytocentrifuge machine for preparing smears from small amount of fluids. Technicians learn it quickly and are quite comfortable with the procedure.
Agarwal
Padam Kumari
PK
Department of Pathology, King George's Medical College, Lucknow, Uttar Pradesh, India. drrajivagarwal@rediffmail.com
D000925:Anticoagulants / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D001798:Blood Proteins; D001826:Body Fluids / Q000166:cytology*; D002469:Cell Separation; D002498:Centrifugation / Q000295:instrumentation; D003581:Cytodiagnosis / Q000295:instrumentation / Q000379:methods*; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes; D006801:Humans; D009369:Neoplasms / Q000097:blood / Q000473:pathology*; D011237:Predictive Value of Tests; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*
D000925:Anticoagulants; D001798:Blood Proteins; D004492:Edetic Acid