Steatosis and hepatic expression of genes regulating lipid metabolism in Japanese patients infected with hepatitis C virus.
OBJECTIVE
Steatosis is a histological finding associated with the progression of chronic hepatitis C. The aims of this study were to elucidate risk factors associated with steatosis and to evaluate the association between steatosis and hepatic expression of genes regulating lipid metabolism.


METHODS
We analyzed 297 Japanese patients infected with hepatitis C virus and a subgroup of 100 patients who lack metabolic factors for steatosis. We determined intrahepatic mRNA levels of 18 genes regulating lipid metabolism in these 100 patients using real-time reverse transcription-polymerase chain reaction. Levels of peroxisome proliferator-activated receptor alpha and sterol regulatory element-binding protein 1 proteins were assessed by immunohistochemistry.


RESULTS
Steatosis was present in 171 (57%) of 297 patients. The presence of steatosis was independently associated with a higher body mass index, higher levels of gamma-glutamyl transpeptidase and triglyceride, and a higher fibrosis stage. Steatosis was present in 43 (43%) of 100 patients lacking metabolic factors. Levels of mRNA and protein of peroxisome proliferator-activated receptor alpha, which regulates beta-oxidation of fatty acid, were lower in patients with steatosis than in patients without steatosis.


CONCLUSIONS
These findings indicate that impaired degradation of lipid may contribute to the development of hepatitis C virus-related steatosis.
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan. yasuik@koto.kpu-m.ac.jp
Kohichiroh
K
Yasui
Yuichi
Y
Harano
Hironori
H
Mitsuyoshi
Kazuhiro
K
Tsuji
Mio
M
Endo
Tomoaki
T
Nakajima
Masahito
M
Minami
Yoshito
Y
Itoh
Yoh
Y
Zen
Yasuni
Y
Nakanuma
Toshikazu
T
Yoshikawa
Takeshi
T
Okanoue
D000328:Adult; D000368:Aged; D005234:Fatty Liver / Q000453:epidemiology / Q000209:etiology* / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006526:Hepatitis C / Q000150:complications* / Q000235:genetics; D006801:Humans; D007564:Japan / Q000453:epidemiology; D050356:Lipid Metabolism / Q000235:genetics*; D008099:Liver / Q000378:metabolism / Q000503:physiopathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D047493:PPAR alpha / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D055815:Young Adult
D047493:PPAR alpha; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1