Global expression profiles in 1-hour biopsy specimens of human kidney transplantation from donors after cardiac death.
Because of the worldwide shortage of renal grafts, kidney transplantation (KTx) from donors after cardiac death (DCD) is an alternative way to obtain KTx from brain-dead donors. Although the prognosis of DCD KTx is gradually improving, the graft often undergoes delayed graft function (DGF), rendering the control of DGF essential for post-KTx patient care. In an attempt to characterize etiology of DGF, genome-wide gene expression profiling was performed using renal biopsy samples performed at 1 h after KTx from DCD and the data were compared with those of KTx from living donors (LD). A total of 526 genes were differentially expressed between them. Genes involved in acute inflammation were activated, while metabolic pathways were consistently downregulated in DCD. These findings imply the inferior performance of the DCD grafts relative to LD grafts. Several genes were identified where the expression levels were correlated well with parameters indicating short- and long-term prognosis of the DCD patients. In addition, several genes encoding secretory proteins were identified that might reflect the performance of the graft and be potential noninvasive biomarkers. These data provide a good source for candidates of biomarkers that are potentially useful for the control of DGF.
Department of Urology, Division of Molecular Genetics, Institute for Comprehensive Medical Science and 21st Century COE Program, Development Center for Targeted and Minimally Invasive Diagnosis and Treatment, Fujita Health University School of Medicine, Aichi 470-1192, Japan. mkusaka@fujita-hu.ac.jp
Mamoru
M
Kusaka
Yoko
Y
Kuroyanagi
Terumi
T
Mori
Kayuri
K
Nagaoka
Hitomi
H
Sasaki
Takahiro
T
Maruyama
Kunihiro
K
Hayakawa
Ryoichi
R
Shiroki
Hiroki
H
Kurahashi
Kiyotaka
K
Hoshinaga
D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D003643:Death; D051799:Delayed Graft Function / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D016030:Kidney Transplantation; D011379:Prognosis; D014019:Tissue Donors
D015415:Biomarkers