Immunomodulatory properties of fermented soy and dairy milks prepared with lactic acid bacteria.
Fermented soy and dairy milk preparations provide a means for delivering lactic acid bacteria and their fermentation products into the diet. Our aims were to test immunomodulatory bioactivity of fermented soy beverage (SB) and dairy milk blend (MB) preparations on human intestinal epithelial cells (IEC) and to determine the impact of freezing medium on culture survival prior to bioactivity analyses. Fermented SB and MB were prepared using pure or mixed cultures of Streptococcus thermophilus ST5, Bifidobacterium longum R0175, and Lactobacillus helveticus R0052. Immunomodulatory bioactivity was assessed by testing selected SB and MB ferments on tumor necrosis factor alpha (TNFalpha)-treated IEC and measuring effects on Interleukin-8 (IL-8) production. Impact of timing of ferment administration relative to this pro-inflammatory challenge was investigated. The most pronounced reductions in IEC IL-8 production were observed when IEC were treated with either SB or MB ferment preparations prior to TNFalpha challenge. These results indicate that freezing-stable MB and SB ferments prepared with selected strains can modulate IEC IL-8 production in vitro, and suggest that yogurt-like fermented soy formulations could provide a functional food alternative to milk-based fermented products.
Wagar
L E
LE
Faculty of Science, Univ. of Ontario Inst. of Technology, Oshawa, ON, Canada.
Champagne
C P
CP
Buckley
N D
ND
Raymond
Y
Y
Green-Johnson
J M
JM
D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development*; D045744:Cell Line, Tumor; D002470:Cell Survival; D018920:Coculture Techniques; D043302:Cultured Milk Products / Q000378:metabolism* / Q000382:microbiology; D004847:Epithelial Cells / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D005615:Freezing; D019073:HT29 Cells; D006801:Humans; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D052198:Lactobacillus helveticus / Q000254:growth & development*; D041942:Soy Milk / Q000008:administration & dosage*; D048249:Streptococcus thermophilus / Q000254:growth & development*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007155:Immunologic Factors; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha