Osteoblastic differentiation of human mesenchymal stem cells with platelet lysate.
Culture of expanded mesenchymal stem cells (MSCs) seeded on biomaterials may represent a clinical alternative to autologous bone graft in bone regeneration. Foetal bovine serum (FBS) is currently used for MSC expansion, despite risks of infectious disease transmission and immunological reaction due to its xenogenic origin. This study aimed to compare the osteogenic capacities of clinical-grade human MSCs cultured with FBS or allogenic human platelet lysate (PL). In vitro, MSCs cultured in PL both accelerate the expansion rate over serial passages and spontaneously induce osteoblastic gene expression such as alkaline phosphatase (ALP), bone sialoprotein (BSP), osteopontin (Op) and bone morphogenetic protein-2 (BMP-2). In vivo, ectopic bone formation is only observed on ceramics seeded with MSCs grown in PL medium implanted under the skin of immunodeficient mice for 7 weeks. In conclusion, allogenic human PL accelerates MSC proliferation and enhances MSC osteogenic differentiation.
EA3952, Cellular and Tissular Bioengineering Laboratory, Henri Mondor Hospital, Paris-EST University, France. nathalie.chevallier@efs.sante.fr
Nathalie
N
Chevallier
Fani
F
Anagnostou
Sebastien
S
Zilber
Gwellaouen
G
Bodivit
Sophie
S
Maurin
Aurelie
A
Barrault
Philippe
P
Bierling
Philippe
P
Hernigou
Pierre
P
Layrolle
Helene
H
Rouard
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002130:Calcium Phosphates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002457:Cell Extracts / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005994:Glycerophosphates / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D010641:Phenotype; D054457:Tissue Scaffolds / Q000737:chemistry
D002130:Calcium Phosphates; D002457:Cell Extracts; D003470:Culture Media; D005994:Glycerophosphates; D017886:Durapatite; C018392:tricalcium phosphate; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid