Mouse models targeting selenocysteine tRNA expression for elucidating the role of selenoproteins in health and development.
Selenium (Se) deficiency has been known for many years to be associated with disease, impaired growth and a variety of other metabolic disorders in mammals. Only recently has the major role that Se-containing proteins, designated selenoproteins, play in many aspects of health and development begun to emerge. Se is incorporated into protein by way of the Se-containing amino acid, selenocysteine (Sec). The synthesis of selenoproteins is dependent on Sec tRNA for insertion of Sec, the 21st amino acid in the genetic code, into protein. We have taken advantage of this dependency to modulate the expression of Sec tRNA that in turn modulates the expression of selenoproteins by generating transgenic, conditional knockout, transgenic/standard knockout and transgenic/conditional knockout mouse models, all of which involve the Sec tRNA gene, to elucidate the intracellular roles of this protein class.
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. carlsonb@mail.nih.gov
Bradley A
BA
Carlson
Min-Hyuk
MH
Yoo
Petra A
PA
Tsuji
Vadim N
VN
Gladyshev
Dolph L
DL
Hatfield
D000818:Animals; D001483:Base Sequence; D048788:Growth and Development; D006262:Health; D051379:Mice; D023421:Models, Animal; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific / Q000737:chemistry / Q000235:genetics*; D051140:Selenoproteins / Q000378:metabolism*
D012345:RNA, Transfer, Amino Acid-Specific; D051140:Selenoproteins; C036928:tRNA, selenocysteine-