Genetic stability, active constituent, and pharmacoactivity of Salvia miltiorrhiza hairy roots and wild plant.
Salvia miltiorrhiza is an annual plant growing in China, Mongolia, Korea and some other Asian countries. The extract from S. miltiorrhiza roots has been used for supporting healthy cardiovascular and circulatory systems during the last decade. The active constituents of S. miltiorrhiza from different areas vary significantly, and the wild resources are overexploited. To adapt the demand for active constituents of S. miltiorrhiza against cardiovascular-related diseases, alternative materials need to be developed. The aim of the present work was to investigate the possibility of S. miltiorrhiza hairy roots as the alternative materials. The results showed that S. miltiorrhiza hairy roots are genetically stable. The contents of salvianolic acid B and tanshinone IIA, two main active constituents in hairy roots, determined by the assessment of combining flow cytometry and phytochemical analysis, are comparable to or significantly lower than in wild plant roots. The extract from S. miltiorrhiza hairy roots also had similar protection activity for hypoxia and reoxygenation injury in rat cardiac myocytes like that from wild plant roots. S. miltiorrhiza hairy roots may be alternative materials to obtain the drug or healthy food for cardiovascular-related diseases.
Yuan
Yuan
Y
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Liu
Yunjun
Y
Lu
Dongmei
D
Huang
Luqi
L
Liang
Rixin
R
Yang
Zhaochun
Z
Chen
Shunqin
S
D045784:Abietanes; D000818:Animals; D001208:Asia; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D018745:Genome, Plant; D006321:Heart / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry / Q000235:genetics*
D045784:Abietanes; D017931:DNA Primers; D004269:DNA, Bacterial; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D010936:Plant Extracts; C036483:T-DNA; C021751:tanshinone