A regulatory domain spanning the repeat sequence RE1 from herpes simplex virus type 1 has cell specific differential functions in trigeminal neurons and fibroblasts.
In this report we demonstrate that the herpes simplex virus type 1 reiteration element 1 (RE1) (nt: 117158-117353) in concert with its flanking sequences is both a cell specific and stimulus inducible regulatory domain. This region of the virus genome and specifically the RE1 supports differential reporter gene expression in both baby hamster kidney cells and disassociated rat trigeminal ganglia and is present within a region that is implicated in regulating latency of the virus in neuronal cells. Further we demonstrate that this locus is a transcriptional regulatory domain and a target for the transcription factor CCCTC binding protein.
School of Biomedical Sciences, Division of Physiology, University of Liverpool, L69 3BX, UK.
Hannah C
HC
Stevens
Carolyn
C
Fiskerstrand
Vivien J
VJ
Bubb
Robert
R
Dalziel
John P
JP
Quinn
D000818:Animals; D001483:Base Sequence; D000076246:CCCTC-Binding Factor; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D017930:Genes, Reporter; D016679:Genome, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology* / Q000378:metabolism; D014775:Virus Activation
D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D012097:Repressor Proteins