Demonstration of x-ray microscopy with an x-ray laser operating near the carbon K edge.
High-brightness and short-pulse-width ( approximately 200 ps) x-ray lasers offer biologists the possibility of high-resolution three-dimensional imaging of specimens in an aqueous environment without the blurring effects associated with natural motions. As a first step toward developing the capabilities of this type of x-ray microscopy we have used a tantalum x-ray laser (lambda = 4.483 nm) together with an x-ray zone plate lens to image a test pattern. The observed image shows a detector-limited resolution of approximately 75 nm and paves the way to three dimensional biological imaging with high spatial resolution (20-30 nm).
L B
LB
Da Silva
J E
JE
Trebes
S
S
Mrowka
T W
TW
Barbee
J
J
Brase
J A
JA
Koch
R A
RA
London
B J
BJ
Macgowan
D L
DL
Matthews
D
D
Minyard
G
G
Stone
T
T
Yorkey
E
E
Anderson
D T
DT
Attwood
D
D
Kern