First isolation of Enterobacter, Enterococcus, and Acinetobacter spp. as inhabitants of the tsetse fly (Glossina palpalis palpalis) midgut.
This paper reports the first evidence of the presence of bacteria, other than the three previously described as symbionts, Wigglesworthia glossinidia, Wolbachia, and Sodalis glossinidius, in the midgut of Glossina palpalis palpalis, the tsetse fly, a vector of the chronic form of human African trypanosomiasis in sub-Saharan African countries. Based on the morphological, nutritional, physiological, and phylogenetic results, we identified Enterobacter, Enterococcus, and Acinetobacter spp. as inhabitants of the midgut of the tsetse fly from Angola. Enterobacter spp. was the most frequently isolated. The role of these bacteria in the gut, in terms of vector competence of the tsetse fly, is discussed, as is the possibility of using these bacteria to produce in situ trypanolytic molecules.
UMR 177, IRD-CIRAD, CIRAD TA A-17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France. anne.geiger@mpl.ird.fr
Anne
A
Geiger
Marie-Laure
ML
Fardeau
Pascal
P
Grebaut
Gedeao
G
Vatunga
Théophile
T
Josénando
Stéphane
S
Herder
Gérard
G
Cuny
Philippe
P
Truc
Bernard
B
Ollivier
D000150:Acinetobacter / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D000810:Angola; D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004754:Enterobacter / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D016983:Enterococcus / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007303:Insect Vectors / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013559:Symbiosis; D014353:Trypanosomiasis, African / Q000635:transmission; D014370:Tsetse Flies / Q000382:microbiology* / Q000502:physiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S