A selective thromboxane A2 (TXA2) synthase inhibitor, ozagrel, attenuates lung injury and decreases monocyte chemoattractant protein-1 and interleukin-8 mRNA expression in oleic acid-induced lung injury in guinea pigs.
This study examined the effect of ozagrel, a thromboxane A(2) synthase inhibitor, on the accumulation of leucocytes and chemokine mRNA expression in lungs experimentally injured using oleic acid (OA). OA injection into guinea pigs rapidly increased thromboxane A(2) generation and subsequently increased total protein concentration and the numbers of macrophages and neutrophils in bronchoalveolar lavage fluid and increased monocyte chemoattractant protein-1 and interleukin-8 mRNA expression in the whole lung. Administration of ozagrel prevented these changes associated with OA injection. Ozagrel is a promising drug candidate for preventing acute lung injury.
Ishitsuka
Yoichi
Y
Department of Clinical Chemistry and Informatics, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Japan.
Moriuchi
Hiroshi
H
Isohama
Yoichiro
Y
Tokunaga
Hidehiro
H
Hatamoto
Keita
K
Kurita
Sumika
S
Irikura
Mitsuru
M
Iyama
Ken-ichi
K
Irie
Tetsumi
T
D055371:Acute Lung Injury / Q000378:metabolism* / Q000517:prevention & control; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D018932:Chemokine CCL2 / Q000378:metabolism*; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D006168:Guinea Pigs; D016209:Interleukin-8 / Q000378:metabolism*; D007958:Leukocyte Count; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008689:Methacrylates; D008751:Methylene Blue / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013928:Thromboxane A2 / Q000037:antagonists & inhibitors*; D013997:Time Factors
D018932:Chemokine CCL2; D016209:Interleukin-8; C043260:May-Grunwald Giemsa; D008689:Methacrylates; D011506:Proteins; D012333:RNA, Messenger; D019301:Oleic Acid; D013928:Thromboxane A2; C034364:ozagrel; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)