Broadening of 1H NMR signals in the spectra of heparin and OSCS by paramagnetic transition metal ions. The use of EDTA to sharpen the signals.
Some signals in the (1)H NMR spectra of heparin and oversulphated chondroitin sulphate (OSCS) are occasionally broad or very broad owing to the presence of paramagnetic metal ions in those polysaccharides. The addition of very small amounts of EDTA to heparin or to OSCS contaminated heparin solutions was needed to obtain normal looking spectra.
McEwen
Ian
I
Medical Products Agency, Box 26, SE-75103 Uppsala, Sweden. ian.mcewen@mpa.se
D002809:Chondroitin Sulfates / Q000032:analysis*; D004492:Edetic Acid / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006493:Heparin / Q000032:analysis*; D007477:Ions; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards; D019216:Metals, Heavy / Q000032:analysis*; D011522:Protons
D007477:Ions; D019216:Metals, Heavy; D011522:Protons; D006493:Heparin; D002809:Chondroitin Sulfates; D004492:Edetic Acid