[Express the recombinant protein PD-L1 in prokaryotic and analyze its biological activity].
OBJECTIVE
To construct the programmed cell death 1 ligant 1 (PD-L1) recombination expression vector, express the fusion protein in prokaryotic and analyze the biological action of express product.


METHODS
The whole PD-L1 gene sequence was synthesized after codon optimized. Construct the thioredoxin-(PD-L1) recombination expression vector and express the fusion protein in E. coli. Purified the target protein and analyze the conjugated ability of protein by ELISA.


RESULTS
The PD-L1 recombinant expression vector has been constructed correctly. The target protein has been obtained with which expressed in high efficiency and production. The target protein can conjugate specifically with the PD-1, its specific receptor.


CONCLUSIONS
We have obtained the PD-L1 recombinant protein success with high biological activity. The result provide the basic condition for further study on antibody and mutually action between PD-L1 and chronic virus infectious.
Shen
Li-Ping
LP
National Institute of Viral Disease Prevention and Control, Chinese CDC , Beijing 100052, China.
Chen
Si-Yong
SY
Bian
Tao
T
Yi
Yao
Y
Wang
Feng
F
Qiu
Feng
F
Bi
Sheng-Li
SL
D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D060890:B7-H1 Antigen; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human; D011993:Recombinant Fusion Proteins