Study on the preservation with CO (PCO = 200-2,000 hPa), resuscitation, and heterotopic transplantation of an isolated rat heart.
In this experiment, CO was used as a gas mixture in a reversible relationship with O(2). CO was added in a gas form mixed with O(2). An isolated donor rat heart was obtained, exposed to a gas mixture such as PO(2) = 1,800 hPa and PCO = 200 hPa, and PO(2) = 1,000 hPa and PCO = 1,000 hPa in a 2 ATA high-pressure chamber and preserved in a refrigerator at 4 degrees C. This report demonstrates that significant reproducibility has been verified. The heart was removed from the refrigerator 24 h later and heterotopic heart transplantation was performed in the right neck of a recipient rat and the pulsating of the transplanted heart was detected by an electrocardiogram.
Yoshida
Yu
Y
Kanagawa University Faculty of Science, Hiratsukashi, 259-1293 Japan. yoshiy412@hotmail.co.jp
Hatayama
Naoyuki
N
Seki
Kunihiro
K
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D005466:Fluorocarbons / Q000494:pharmacology; D006321:Heart / Q000502:physiology*; D016027:Heart Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D012151:Resuscitation
D005466:Fluorocarbons; D002248:Carbon Monoxide