Analysis of meiotic recombination products from human sperm.
Traditional methods for surveying meiotic recombination in humans are limited to pedigree and linkage disequilibrium analyses. We have developed assays that allow the direct detection of crossover and gene conversion molecules in batches of sperm DNA. To date, we have characterized 26 recombination hotspots by allele-specific PCR and selectively amplified recombinant DNA molecules from these regions. These analyses have revealed that meiotic crossover hotspots in humans are highly localized and flanked by DNA segments where recombination is suppressed. The centers of crossover hotspots are also active in noncrossover recombination, displaying short conversion tracts.
Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Liisa
L
Kauppi
Celia A
CA
May
Alec J
AJ
Jeffreys
D000465:Algorithms; D020732:Cytogenetic Analysis / Q000379:methods*; D006801:Humans; D008297:Male; D008540:Meiosis / Q000235:genetics*; D008954:Models, Biological; D016133:Polymerase Chain Reaction / Q000379:methods; D011995:Recombination, Genetic / Q000235:genetics*; D013094:Spermatozoa / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*