[Expression of Bcl-2 and Bax proteins in myocardial cells after Macleaya cordata alkaloids poisoning in rats].
OBJECTIVE
To observe the expression of Bcl-2 and Bax proteins in rat's myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning.


METHODS
Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system.


RESULTS
The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05).


CONCLUSIONS
If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.
Institute of Forensic Science, Gongli Hospital of Pudong New District, Shanghai 200135, China. zhangdy32@126.com
De-Yu
DY
Zhang
Qing-Quan
QQ
Kong
Chun-Bing
CB
Zhang
Jian-Hua
JH
Zhu
Ming-Di
MD
Chen
Peng
P
Wang
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D028101:Papaveraceae / Q000737:chemistry*; D010208:Papaverine / Q000008:administration & dosage / Q000506:poisoning*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D010208:Papaverine