Phytoconstituents of Jatropha curcas L. leaves and their immunomodulatory activity on humoral and cell-mediated immune response in chicks.
A novel biflavone di-C-glucoside, 6,6"-di-C-beta-D-glucopyranoside-methylene-(8,8")-biapigenin (1), was isolated from the leaves of Jatropha curcas L. (Euphorbiaceae), together with six known compounds; apigenin 7-O-beta-D-neohesperidoside (2), apigenin 7-O-beta-D-galactoside (3), orientin (4), vitexin (5), vicenin II (6), and apigenin (7). Their structures were determined on the basis of extensive chemical and spectroscopic analyses (UV, NMR and HRESI-MS). The immunomodulatory effect of an 80% aqueous methanol extract (AME) and compounds 1-5 (0.25 mg/kg body wt) to one-day-old specific pathogen-free (SPF) chicks was determined. Stimulation of both humoral and cell-mediated seroresponse was observed, especially those of AME and compound 1. Remarkable effective increases of the antibody titers, lymphocyte and macrophage cells, in blood were recorded. SPF chicks treated with the tested samples exhibited protection against Newcastle disease challenge virus after being vaccinated.
Chemistry of Natural Compounds Department, National Research Centre (NRC), Dokki, Egypt
Howaida I
HI
Abd-Alla
Fatma A
FA
Moharram
Ahmed H
AH
Gaara
Mounir M
MM
El-Safty
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002645:Chickens; D004534:Egypt; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D007111:Immunity, Cellular / Q000187:drug effects*; D007155:Immunologic Factors / Q000302:isolation & purification* / Q000494:pharmacology*; D031286:Jatropha / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects / Q000276:immunology; D008099:Liver / Q000187:drug effects / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D009521:Newcastle Disease / Q000276:immunology; D009522:Newcastle disease virus / Q000276:immunology; D018515:Plant Leaves / Q000737:chemistry*; D013047:Specific Pathogen-Free Organisms; D014765:Viral Vaccines / Q000627:therapeutic use; D014774:Virulence
D047309:Flavones; D007155:Immunologic Factors; D014765:Viral Vaccines