Single-step enzymatic synthesis of (R)-2-O-alpha-D-glucopyranosyl glycerate, a compatible solute from micro-organisms that functions as a protein stabiliser.
Regioselective glucosylation of R-glycerate catalysed by sucrose phosphorylase in the presence of sucrose as the donor substrate provided the natural compatible solute (R)-2-O-alpha-D-glucopyranosyl glycerate with complete regioselectivity in an optimised synthetic yield of 90% R-glycerate converted and a concentration of about 270 mM.
Sawangwan
Thornthan
T
Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/1, Graz, Austria.
Goedl
Christiane
C
Nidetzky
Bernd
B
D055162:Biocatalysis; D005960:Glucosides / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000378:metabolism*; D005988:Glyceric Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006878:Hydroxides / Q000378:metabolism; D007934:Leuconostoc / Q000201:enzymology; D055550:Protein Stability / Q000187:drug effects; D013237:Stereoisomerism; D013379:Substrate Specificity; D013395:Sucrose / Q000378:metabolism
C509251:2-glucosylglycerate; D005960:Glucosides; D005988:Glyceric Acids; D006878:Hydroxides; D013395:Sucrose; C042971:glyceric acid; C031356:hydroxide ion; D005964:Glucosyltransferases; C025901:sucrose phosphorylase