In silico DNA vaccine designing against human papillomavirus (HPV) causing cervical cancer.
HPV vaccines available in the market are not effective against different strains of papillomavirus, therefore, there is a need to develop a new prophylactic DNA vaccine which can work against different strains of HPVs and may lead to protection of cervical cancer against new pandemic viruses. We designed a potential prophylactic DNA vaccine by using all the consensus epitopic sequences of HPVs L2 capsid protein and performed in silico cloning of multiepitopic antigenic DNA sequence in pVAX-1 vector. Immunogenicity of vaccine has been enhanced by techniques like codon optimization, engineering CpG motifs, introducing promoters and co-injection with plasmids expressing immune-stimulatory molecules.
Gupta
Shishir Kumar
SK
Society for Biological Research & Rural Development, Lucknow 226010, UP, India. shishir.bioinfo@gmail.com
Singh
Archana
A
Srivastava
Mugdha
M
Gupta
Shailendra K
SK
Akhoon
Bashir Akhlaq
BA
D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D018899:CpG Islands; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D016366:Open Reading Frames; D030361:Papillomavirus Infections / Q000276:immunology / Q000517:prevention & control*; D053918:Papillomavirus Vaccines / Q000276:immunology*; D010802:Phylogeny; D016415:Sequence Alignment; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology
D000956:Antigens, Viral; D019496:Cancer Vaccines; D036022:Capsid Proteins; D000939:Epitopes; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; D019444:Vaccines, DNA