Expression of HIF-1 alpha, VEGF and EPO in peripheral blood from patients with two cardiac abnormalities associated with hypoxia.
OBJECTIVE
HIF-1 alpha (hypoxia-inducible factor-1 alpha) mediates the responses of mammalian cells to hypoxia/ischemia by inducing the expression of adaptive gene products (e.g., vascular endothelial growth factor (VEGF) and erythropoietin (EPO)). Persistent pulmonary hypertension of the newborn (PPHN) and cyanotic congenital heart disease (CCHD) are common neonatal diseases considered as paradigms of hypoxemia. Since the expression HIF-1 alpha, VEGF and EPO in newborns diagnosed with these diseases has yet to be studied, we set out to define the expression of these genes in peripheral blood from newborn infants diagnosed with PPHN and CCHD.


METHODS
The mRNA transcripts encoding HIF-1 alpha, VEGF and EPO were measured by RT-PCR in healthy newborn infants and infants diagnosed with PPHN and CCHD.


RESULTS
An important increase in HIF-1 alpha expression was observed in both pathological conditions, accompanied by significant increases in VEGF and EPO expression when compared to healthy infants.


CONCLUSIONS
HIF-1 alpha mRNA expression increases in newborn infants with PPHN or CCHD, as does the expression of its target genes VEGF and EPO.
Lemus-Varela
M L
ML
Neonatología, Hospital de Pediatría UMAE del CMNO, IMSS, Guadalajara, Jalisco, Mexico.
Flores-Soto
M E
ME
Cervantes-Munguía
R
R
Torres-Mendoza
B M G
BM
Gudiño-Cabrera
G
G
Chaparro-Huerta
V
V
Ortuño-Sahagún
D
D
Beas-Zárate
C
C
D004921:Erythropoietin / Q000097:blood / Q000235:genetics; D006331:Heart Diseases / Q000097:blood / Q000151:congenital / Q000503:physiopathology; D006801:Humans; D000860:Hypoxia / Q000097:blood / Q000473:pathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000097:blood / Q000235:genetics; D007223:Infant; D007231:Infant, Newborn; D010547:Persistent Fetal Circulation Syndrome / Q000097:blood / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000235:genetics
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A; D004921:Erythropoietin