Determination of amphetamine and methamphetamine in umbilical cord using liquid chromatography-tandem mass spectrometry.
The use of meconium as a drug-screening matrix for newborns has been the gold standard of care for the past two decades. A recent study using matched pairs of meconium and umbilical cord demonstrated a high degree of agreement. The use of liquid chromatography-tandem mass spectrometry as a means to confirm amphetamines presumptive positive umbilical cord specimens for amphetamine and methamphetamine is described here for the first time. The limit of detection for both compounds was 0.2 ng/g. The limit of quantitation for both compounds was 0.6 ng/g. The assay was linear for both compounds up to 100 ng/g.
United States Drug Testing Laboratories, Des Plaines, IL 60018, United States. joe.jones@usdtl.com
Joseph
J
Jones
Rosemarie
R
Rios
Mary
M
Jones
Douglas
D
Lewis
Charles
C
Plate
D000661:Amphetamine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008694:Methamphetamine / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014470:Umbilical Cord / Q000737:chemistry*
D008694:Methamphetamine; D000661:Amphetamine