[Screening and identification of polypeptides specifically binding to human B type natriuretic peptide from 12TM phage display peptide library].
OBJECTIVE
To identify and characterize the polypeptides specifically binding to human B type natriuretic peptide (BNP) screened from 12TM phage display peptide library.


METHODS
The BNP-binding peptides were screened from 12TM phage display peptide library and identified by ELISA.


RESULTS
After 4 rounds of screening, 10 of the 16 phage clones were identified as the positive clones which could bind to BNP. Five amino acid sequences were obtained in the 10 positive clones. Dose-dependent ELISA results demonstrated that the screened polypeptides could specifically bind to BNP.


CONCLUSIONS
These screened polypeptides can bind specifically to BNP, which provides a basis for further research on expression and purification of anti-BNP polypeptides and the development of the detection kit of BNP.
Zhang
Zhen-hui
ZH
Intensive Care Unit, Second Affiliated Hospital of Guangzhou Medical College, Guangzhou, China. drzhzhh@126.com
Liu
Shi-ming
SM
Wu
Wen-yan
WY
Zhang
Huan-tang
HT
Zheng
Wei-nan
WN
D006801:Humans; D020097:Natriuretic Peptide, Brain / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding
D019151:Peptide Library; D010455:Peptides; D020097:Natriuretic Peptide, Brain