Modulating and targeting meiotic double-strand breaks in Saccharomyces cerevisiae.
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10-100-fold from site to site. Herein are reviewed the methods used in budding yeast to modulate locally and globally the native DSB frequencies, including a powerful method to target Spo11-dependent meiotic DSB in novel chromosomal regions. These methods serve to investigate the control and the mechanism of recombination initiation and modify the natural distribution of meiotic recombination.
Nicolas
Alain
A
Institut Curie, Centre de Recherche, UMR7147-CNRS, Université Pierre et Marie Curie, Paris, France.
D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics / Q000502:physiology*; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D004706:Endodeoxyribonucleases; D018390:Gene Targeting / Q000379:methods; D008540:Meiosis / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D030781:Organisms, Genetically Modified; D011995:Recombination, Genetic / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D000099257:Meiotic Recombination Protein SPO11
D004250:DNA Topoisomerases, Type II; D004706:Endodeoxyribonucleases; D029701:Saccharomyces cerevisiae Proteins; C518938:Spo11 protein, S cerevisiae; D000099257:Meiotic Recombination Protein SPO11