Wistar rat skin as surrogate for human skin in nortriptyline hydrochloride patch studies.
Six different matrices were prepared containing nortriptyline hydrochloride (NTH) with hydroxypropyl-methyl-cellulose as polymer. A mixture of transdermal enhancers was included as part of the vehicle. Diffusion studies were carried out through Wistar rat full thickness skin using Franz cells. They were compared with previously determined human heat separated epidermis in order to test if this animal can be used as model for in vivo assays. A linear correlation was obtained between NTH diffusion coefficients through both skin types (r2=0.996).
Department of Pharmacy and Pharmaceutics, University of Valencia, Av. VA Estellés, s/n, 46100 Burjassot, Valencia, Spain.
Ana
A
Melero
Claus-Michael
CM
Lehr
Ulrich F
UF
Schäfer
Teresa M
TM
Garrigues
D000279:Administration, Cutaneous; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D023421:Models, Animal; D009661:Nortriptyline / Q000008:administration & dosage* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology
D009661:Nortriptyline