BchY-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR.
To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.
Yutin
Natalya
N
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA. yutin@ncbi.nlm.nih.gov
Suzuki
Marcelino T
MT
Rosenberg
Mira
M
Rotem
Denisse
D
Madigan
Michael T
MT
Süling
Jörg
J
Imhoff
Johannes F
JF
Béjà
Oded
O
D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D008969:Molecular Sequence Data; D010788:Photosynthesis; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment
D001426:Bacterial Proteins; D017931:DNA Primers