[Community structure and kinetics characterization of enriched nitrifiers cultivated with reject water].
The community of nitrifiers cultivated with reject water were investigated by fluorescent in situ hybridization (FISH) performed with 16S rRNA-targeted oligonucleotide probes, and the kinetic properties such as temperature correction factor tauN and the half saturation coefficient K(N) were also investigated. The results showed that the percentage of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) in the activated sludge was 15.7% +/- 3.7% and 12.9% +/- 3.2%, Nitrosomonas europaea-Nitrosococcus mobilis lineage were the dominant AOB, Nitrobacter spp. were the dominant NOB. The temperature correction factors of ammonium utilized rate (AUR) and nitrite utilized rate (NUR) were 1.092 and 1.061 respectively; the K(N) of AOB and NOB were (1.60 +/- 0.29) mg/L (NH4(+) -N), (2.78 +/- 0.30) mg/L (NO2(-) -N) respectively.
Key Laboratory of Northwest Water Resources, Environment and Ecology of Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China. yulifang81@163.com
Li-Fang
LF
Yu
Qing-Qing
QQ
Chen
Jin
J
Yang
Dang-Cong
DC
Peng
D007218:Industrial Microbiology; D007700:Kinetics; D009573:Nitrites / Q000378:metabolism; D009576:Nitrobacter / Q000254:growth & development* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009606:Nitrosomonas / Q000254:growth & development* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*
D009573:Nitrites; D000644:Quaternary Ammonium Compounds; D012722:Sewage; D009584:Nitrogen