Gap junctions and memory: an investigation using a single trial discrimination avoidance task for the neonate chick.
Gap junctions are important to how the brain functions but are relatively under-investigated with respect to their contribution towards behaviour. In the present study a single trial discrimination avoidance task was used to investigate the effect of the gap junction inhibitor 18-alpha-glycyrrhetinic acid (alphaGA) on retention. Past studies within our research group have implied a potential role for gap junctions during the short-term memory (STM) stage which decays by 15 min post-training. A retention function study comparing 10 microM alphaGA and vehicle given immediately post-training demonstrated a significant main effect for drug with retention loss at all times of test (10-180 min post-training). Given that the most common gap junction in the brain is that forming the astrocytic network it is reasonable to conclude that alphaGA was acting upon these. To confirm this finding and interpretation two additional investigations were undertaken using endothelin-1 (ET-1) and ET-1+tolbutamide. Importantly, a retention function study using 10nM ET-1 replicated the retention loss observed for alphaGA. In order to confirm that ET-1 was acting on astrocytic gap junctions the amnestic action of ET-1 was effectively challenged with increasing concentrations of tolbutamide. The present findings suggest that astrocytic gap junctions are important for memory processing.
Verwey
L J
LJ
School of Psychology, Psychiatry and Psychological Medicine, Monash University, 3800 Victoria, Australia. lucyverwey@hotmail.com
Edwards
T M
TM
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology*; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D002491:Central Nervous System Agents / Q000008:administration & dosage / Q000494:pharmacology; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000378:metabolism; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D008570:Memory, Short-Term / Q000187:drug effects / Q000502:physiology; D009483:Neuropsychological Tests; D011897:Random Allocation; D013997:Time Factors; D014044:Tolbutamide / Q000008:administration & dosage / Q000494:pharmacology
D002491:Central Nervous System Agents; D019332:Endothelin-1; C119129:18alpha-glycyrrhetinic acid; D014044:Tolbutamide; D006034:Glycyrrhetinic Acid