[Comparison of pretreatment methods for the simultaneous determination of diclazuril and toltrazuril residues in chicken tissues].
The effects of four pretreatment methods (acetonitrile extraction-evaporation concentration, acetonitrile extraction-solid phase extraction (SPE), matrix solid-phase dispersion (MSPD) extraction and MSPD-SPE) for the simultaneous analysis of diclazuril and toltrazuril residues in chicken tissues were compared. The average recovery of 70% for the former three methods as achieved. In comparison with other methods, the MSPD method saved more than 60% in time and solvent. So, MSPD as the sample pretreatment method, an MSPD-high performance liquid chromatography with ultraviolet detection (MSPD-HPLC/UV) method was established for the analysis. Under the optimal chromatographic conditions, the linear range was between 50 and 1,000 microg/kg. At the added levels of 50, 500, 1,000 ng/g, the recoveries of diclazuril and toltrazuril in chicken tissues ranged from 71.13% - 84.02% with the relative standard deviations (RSD) in the range of 3.76% - 12.11%, and the RSDs of intra- and interday analyses ranged from 3.70% - 6.77%. The detection limits of diclazuril and toltrazuril were less than 10 microg/kg. The quantitative limits of diclazuril and toltrazuril were less than 20 microg/kg. The method meet the requirements of the residue analysis on accuracy and precision.
Shi
Zuhao
Z
College of Animal Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Ge
Qinglian
Q
Lu
Junxian
J
Liu
Xuexian
X
Gong
Jiansen
J
Zhu
Liangqiang
L
Qi
Kezong
K
Chen
Dingding
D
Peng
Kaisong
K
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008461:Meat Products / Q000032:analysis*; D009570:Nitriles / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D014227:Triazines / Q000032:analysis*
D003049:Coccidiostats; D009570:Nitriles; D014227:Triazines; C057884:diclazuril; C036670:toltrazuril