A trial of alveolar cleft bone regeneration by controlled release of bone morphogenetic protein: an experimental study in rabbits.
OBJECTIVE
Autologous bone grafting is a currently preferred method for alveolar cleft bone regeneration. However, there are some disadvantages to this technique, including the limited availability of donor sites. In this study, we introduce a novel graft material of gelatin hydrogel enabling the controlled release of bone morphogenetic protein 2 (BMP-2) for alveolar cleft bone regeneration.


METHODS
Gelatin hydrogels incorporating BMP-2 or BMP-2-free solution and BMP-2 solution were applied to experimental alveolar clefts prepared in the maxillary bone of rabbits. As an additional control, the alveolar clefts were left untreated. Bone regeneration at the alveolar clefts was evaluated by microfocus computerized tomographic, histologic, and histomorphometric examinations.


RESULTS
Significant bone regeneration was observed in the alveolar clefts treated with gelatin hydrogels incorporating BMP-2 compared with other groups.


CONCLUSIONS
Gelatin hydrogels incorporating BMP-2 is a promising material for the bone regeneration of alveolar clefts.
First Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka Dental University, Osaka, Japan.
Yasunori
Y
Sawada
Akishige
A
Hokugo
Aki
A
Nishiura
Rie
R
Hokugo
Naoyuki
N
Matsumoto
Shosuke
S
Morita
Yasuhiko
Y
Tabata
D020341:Absorbable Implants; D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology / Q000601:surgery; D000543:Alveoloplasty; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D018786:Bone Substitutes / Q000008:administration & dosage; D004343:Drug Implants / Q000008:administration & dosage*; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D020100:Hydrogels / Q000008:administration & dosage; D008437:Maxilla / Q000000981:diagnostic imaging / Q000502:physiology / Q000601:surgery; D016348:Osseointegration / Q000187:drug effects; D010027:Osteotomy; D011817:Rabbits; D011994:Recombinant Proteins; D018709:Statistics, Nonparametric; D055114:X-Ray Microtomography
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D004343:Drug Implants; D020100:Hydrogels; D011994:Recombinant Proteins