Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution.
The tyrosine phenol-lyase from Symbiobacterium toebii was engineered to improve both its stability and catalytic activity by the application of random mutagenesis and subsequent reassembly of the acquired mutations. Activity screening of the random library produced four mutants with a two-fold improved activity, whereas parallel screening after heat treatment at 65 degrees C identified three mutants with half-inactivation temperatures improved by up to 5.6 degrees C. The selected mutants were then reassembled using the staggered extension PCR method, and subsequent screening of the library produced seven mutants with up to three-fold improved activity and half-inactivation temperatures improved by up to 11.2 degrees C. Sequence analyses revealed that the stability-improved hits included A13V, E83K and T407A mutations, whereas the activity-improved hits included the additional T129I or T451A mutation. In particular, the A13V mutation was propagated in the hits with improved stability during the reassembly-screening process, indicating the critical nature of the N-terminal moiety for enzyme stability. Furthermore, homology modeling of the enzyme structure revealed that most of the stability mutations were located around the dimer-dimer interface, including the N-terminus, whereas the activity-improving mutations were located further away, thereby minimizing any interference that would be detrimental to the co-improvement of the stability and catalytic activity of the enzyme.
Rha
Eugene
E
Industrial Biotechnology & Bioenergy Research Center, KRIBB, Daejeon, South Korea.
Kim
Sujin
S
Choi
Su-Lim
SL
Hong
Seung-Pyo
SP
Sung
Moon-Hee
MH
Song
Jae J
JJ
Lee
Seung-Goo
SG
D039903:Actinobacteria / Q000201:enzymology; D000595:Amino Acid Sequence; D002384:Catalysis; D019020:Directed Molecular Evolution / Q000379:methods*; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011487:Protein Conformation; D014447:Tyrosine Phenol-Lyase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D014447:Tyrosine Phenol-Lyase