[Investigation on distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai].
OBJECTIVE
To explore the distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai.


METHODS
Nested polymerase chain reaction (nPCR) was used for amplification of S gene and C gene of HBV from sera carried by Tibetan chronic HBV carrier in Tongde, Qinghai, then the HBV DNA positive products were sequenced by direct sequencing. Genotype and serotype were identified by analysis of sequence result.


RESULTS
271, which come from 311 sera samples with positive HBsAg randomly selected from natural community, were amplified and sequenced in both S gene and C gene successfully, 10 (3.7%), 261 (96.3%) out of them were identified as genotype C, recombinant between genotypes C and D respectively; 259 (95.6%), 10 (3.7%), 2 (0.7%) belonged to serotype ayw2, adr, adw2 respectively.


CONCLUSIONS
The recombinant between genotypes C and D was the main genotype in Tibetan chronic carrier with hepatitis Bin Tongde, Qinghai; the serotype of this areas was consisted largely of ayw2.
Department of Epidemiology and Healthy Statistics, Hebei Medical University, Shijiazhuang 050017, China.
Jie
J
Zang
Shuang-Ying
SY
Jiang
Xue-Wen
XW
Wang
Feng
F
Wang
Tao
T
Bian
Zhen-Yun
ZY
Mu
Yong
Y
Zhang
D000293:Adolescent; D000328:Adult; D002681:China; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood; D006509:Hepatitis B / Q000276:immunology / Q000821:virology*; D006512:Hepatitis B Core Antigens / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D006508:Hepatitis Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens