Design of Eudragit RL 100 nanoparticles by nanoprecipitation method for ocular drug delivery.
The objective of the current study was to prepare positively charged amphotericin-B-loaded nanoparticles providing a controlled release formulation. The particles were prepared by solvent displacement or nanoprecipitation method. The non-biodegradable positively charged polymer Eudragit RL 100 was used to prepare the different formulations with varying ratios of drug and polymer. The formulations were evaluated in terms of particle size, zeta potential, and differential scanning calorimetry measurements. Drug entrapment and release properties were examined also. The antimicrobial activity against Fusarium solani was determined. In vivo eye irritation study was carried out by a modified Draize test. All the formulations remained within a size range of 130 to 300 nm in fresh preparation as well as after 2 months. The zeta potential was positive (+22 to +42 mV) for all the formulations and was suitable for ophthalmic application. A prolonged drug release was shown by all the formulations. The formulation possesses a good antifungal activity against Fusarium solani when tested by disk diffusion method, and no eye irritation on in vivo testing was found.


BACKGROUND
The objective of the current study was to prepare positively charged amphotericin-B-loaded nanoparticles providing a controlled release formulation. The described formulation displayed good antifungal activity against Fusarium solani when tested by disk diffusion method, and no eye irritation on in vivo testing was found.
Das
Swarnali
S
School of Pharmacy and Technology Management, NMIMS University, Mumbai, Maharastra, India. swarnali4u@rediffmail.com
Suresh
Preeti K
PK
Desmukh
Rohitas
R
D000180:Acrylic Resins / Q000737:chemistry*; D000666:Amphotericin B / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D015195:Drug Design; D015821:Eye Infections, Fungal / Q000188:drug therapy*; D005590:Fractional Precipitation / Q000379:methods; D008297:Male; D049329:Nanostructures / Q000737:chemistry / Q000627:therapeutic use*; D009883:Ophthalmic Solutions / Q000008:administration & dosage* / Q000737:chemistry*; D011817:Rabbits; D016896:Treatment Outcome
D000180:Acrylic Resins; D000935:Antifungal Agents; D003692:Delayed-Action Preparations; D009883:Ophthalmic Solutions; C068538:liposomal amphotericin B; C050528:Eudragit RS; D000666:Amphotericin B