[Expression of oxyntomodulin in bifidobacteria and effect of oxyntomodulin-transformed bifidobacteria on the body weight of obese mice].
OBJECTIVE
To observe the effect of pBBADs-OXM-transformed bifidobacteria on the body weight of obese mice.


METHODS
B. longum was transformed with pBBADs-OXM by electroporation, and arabopyranose-induced oxyntomodulin expression by the bacterium was detected by ELISA. pBBADs-OXM-transformed bifidobacteria was administered orally obese mice on a daily basis with pBBADs-GFP-transformed bifidobacteria as the negative control, and the body weight changes of the mice were observed.


RESULTS
OXM was detected by ELISA not only in the supernatant but also the precipitant of the transformed bacterial culture. The body weight of the obese mice fed with pBBADs-OXM-transformed bifidobacteria decreased significantly compared with that of the mice in the obese model group (P<0.05).


CONCLUSIONS
Administration of pBBADs-OXM-transformed B.longum can reduce the body weight of obese mice.
Department of Cell Biology, Southern Medical University, Guangzhou, China. longruoting@yahoo.com.cn
Ruo-ting
RT
Long
Wei-sen
WS
Zeng
Shen-qiu
SQ
Luo
Jiao
J
Guo
Yuan-zao
YZ
Lin
Qing-song
QS
Huang
D000284:Administration, Oral; D000818:Animals; D001067:Appetite Depressants / Q000008:administration & dosage / Q000378:metabolism; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009765:Obesity / Q000188:drug therapy*; D053772:Oxyntomodulin / Q000008:administration & dosage* / Q000096:biosynthesis* / Q000235:genetics; D011897:Random Allocation; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics
D001067:Appetite Depressants; D053772:Oxyntomodulin; D011994:Recombinant Proteins