Selective neuronal requirement for huntingtin in the developing zebrafish.
Huntington's disease shares a common molecular basis with eight other neurodegenerative diseases, expansion of an existing polyglutamine tract. In each case, this repeat tract occurs within otherwise unrelated proteins. These proteins show widespread and overlapping patterns of expression in the brain and yet the diseases are distinguished by neurodegeneration in a specific subset of neurons that are most sensitive to the mutation. It has therefore been proposed that expansion of the polyglutamine region in these genes may result in perturbation of the normal function of the respective proteins, and that this perturbation in some way contributes to the neuronal specificity of these diseases. The normal functions of these proteins have therefore become a focus for investigation as potential pathogenic pathways. We have used synthetic antisense morpholinos to inhibit the translation of huntingtin mRNA during early zebrafish development and have previously reported the effects of huntingtin reduction on iron transport and homeostasis. Here we report an analysis of the effects of huntingtin loss-of-function on the developing nervous system, observing distinct defects in morphology of neuromasts, olfactory placode and branchial arches. The potential common origins of these defects were explored, revealing impaired formation of the anterior-most region of the neural plate as indicated by reduced pre-placodal and telencephalic gene expression with no effect on mid- or hindbrain formation. These investigations demonstrate a specific 'rate-limiting' role for huntingtin in formation of the telencephalon and the pre-placodal region, and differing levels of requirement for huntingtin function in specific nerve cell types.
ARC Special Research Centre for the Molecular Genetics of Development and Discipline of Genetics, School of Molecular and Biomedical Sciences, The University of Adelaide, Adelaide, SA, Australia.
Tanya L
TL
Henshall
Ben
B
Tucker
Amanda L
AL
Lumsden
Svanhild
S
Nornes
Michael T
MT
Lardelli
Robert I
RI
Richards
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology / Q000502:physiology; D002356:Cartilage / Q000166:cytology / Q000254:growth & development; D002454:Cell Differentiation; D055785:Gene Knockdown Techniques; D006801:Humans; D006816:Huntington Disease / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009432:Neural Crest / Q000166:cytology / Q000254:growth & development; D054258:Neural Plate / Q000254:growth & development; D055495:Neurogenesis / Q000235:genetics*; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000502:physiology*; D013687:Telencephalon / Q000254:growth & development* / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development*; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
D019208:Brain-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; D029961:Zebrafish Proteins; C524778:htt protein, zebrafish