Enhanced protein immobilization efficiency on a TiO2 surface modified with a hydroxyl functional group.
An antibody immobilization was investigated using a self-assembled monolayer (SAM) over the highly refractive coatings with a SiO2, TiO2, or Si3N4 substrate. The immobilization was characterized by analyzing the hydrophilic properties of hydroxyl (OH) groups on surface coatings with contact angle (CA) measurements to enhance protein immobilization. The hydroxyl (OH) group was formed in greater amounts as the oxygen plasma exposure time was increased, which resulted in a large enhancement in antibody immobilization. It indicated that hydroxyl (OH) group formation is critical for developing a label-free optical transducer with a high sensitivity.
Kim
Wan-Joong
WJ
Biosensor Research Team, Electronics and Telecommunications Research Institute, Daejeon 305-700, South Korea. kokwj@etri.re.kr
Kim
Sanghee
S
Lee
Bong Soo
BS
Kim
Ansoon
A
Ah
Chil Seong
CS
Huh
Chul
C
Sung
Gun Yong
GY
Yun
Wan Soo
WS
D000588:Amines / Q000737:chemistry; D000818:Animals; D055910:Antibodies, Immobilized / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D057927:Hydrophobic and Hydrophilic Interactions; D006878:Hydroxides / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000276:immunology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
D000588:Amines; D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D006878:Hydroxides; C009495:titanium dioxide; C031356:hydroxide ion; D014025:Titanium; D017430:Prostate-Specific Antigen; D010100:Oxygen