Current-driven ion fluxes of polymeric membrane ion-selective electrode for potentiometric biosensing.
A polymeric membrane ion-selective electrode (ISE) for rapid potentiometric biosensing has been developed. Butyrylcholinesterase, as a target enzyme, is immobilized on the surface of the polymeric membrane. A macro-command-controlled procedure for switching between the potentiostatic and galvanostatic steps has been designed to provide a current-driven release of the butyrylcholine substrate for in situ biosensing of the enzyme and its inhibitors. This system has the flexibility of trapping substrate ions in the ISE inner filling solution and provides rapid, continuous, and reproducible measurements of enzymes and other bioanalytes involved in enzymatic systems.
Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai 264003, PR China.
Jiawang
J
Ding
Wei
W
Qin
D015374:Biosensing Techniques / Q000379:methods*; D002091:Butyrylcholinesterase / Q000032:analysis / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000032:analysis; D004553:Electric Conductivity; D004566:Electrodes; D007477:Ions / Q000378:metabolism; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011199:Potentiometry
D002800:Cholinesterase Inhibitors; D007477:Ions; D008567:Membranes, Artificial; D011108:Polymers; D002091:Butyrylcholinesterase