Growth curves of Escherichia coli and Pseudomonas aeruginosa submitted to different ceftazidime concentrations.
Cultures of Escherichia coli and Pseudomonas aeruginosa have been submitted to different ceftazidime concentrations (from 1 to 500 mg/l). A continuous monitoring of the culture was made with the MS-2 system. Phase contrast microscopy was used for study of bacterial morphology. The data obtained showed: for E. coli a drop in optical density and formation of round forms at concentrations close to the minimum inhibitory concentration (MIC) and for Ps. aeruginosa, a residual growth at all concentrations tested during the first 2 h. This corresponded to filament formation. These observations might have clinical implications concerning the mode of administration of the antibiotic, i.e., bolus or continuous infusion.
Department of Clinical Biology, Brugmann University Hospital, 4, Place Van Gehuchten, 1020 Bruxelles, Belgium.
E
E
Yourassowsky
M P
MP
Van der Linden
M L
ML
Lismont
F
F
Crokaert
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008858:Microscopy, Phase-Contrast; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development*
D000900:Anti-Bacterial Agents; D002442:Ceftazidime