Arthrobacter antarcticus sp. nov., isolated from an Antarctic marine sediment.
A bacterial strain, SPC26(T), was isolated from a sediment sample of the Southern Ocean off Antarctica. The strain was Gram-staining- and catalase-positive and contained lysine and alanine in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C₁₅:₀ (54.92 %), iso-C₁₅:₀ (11.47 %), anteiso-C₁₇:₀ (6.48 %) and anteiso-C₁₅:₁ (6.38 %) and the major menaquinones were MK-8, MK-9 and MK-10. The major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. The G+C content was 68 ± 0.5 mol%. Based on 16S rRNA gene sequence similarities, the nearest phylogenetic neighbours of strain SPC26(T) were identified as Arthrobacter gangotriensis Lz1y(T) (98.8 %), A. sulfureus DSM 20167(T) (98.6 %), A. psychrophenolicus DSM 15454(T) (97.9 %) and A. kerguelensis KGN15(T) (97.5). With these strains, strain SPC26(T) exhibited DNA-DNA relatedness values of 36, 21, 12 and 10 %, respectively. Therefore, on the basis of 16S rRNA gene sequence comparisons, phylogenetic analysis, phenotypic characteristics and DNA-DNA relatedness, it is proposed that strain SPC26(T) represents a novel species of Arthrobacter, for which the name Arthrobacter antarcticus sp. nov. is proposed, with strain SPC26(T) (=LMG 24542(T) =NCCB 100228(T)) as the type strain.
Pindi
Pavan Kumar
PK
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Manorama
Ruth
R
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Begum
Zareena
Z
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Shivaji
S
S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
D000409:Alanine / Q000032:analysis; D000864:Antarctic Regions; D001173:Arthrobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008239:Lysine / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D002374:Catalase; D008239:Lysine; D000409:Alanine