The influence of dietary habits and pathological conditions on the binding of theophylline to serum albumin.
The influence of fatty acids (FA) on theophylline (Th) binding to human serum albumin (HSA) in its high and low affinity binding sites was investigated. The content of studied FA solutions corresponds to the ones associating with different dietary habits and pathological states in vivo. Using fluorescence and (1)H NMR spectroscopy two high and two low affinity binding sites of Th in HSA structure were found. For each site several binding parameters in the absence and presence of FA were estimated. The results showed that the impact of FA on the affinity of HSA towards Th in high affinity binding sites is negligible whereas binding of the drug in low affinity sites decreases significantly in the presence of FA. It was observed that this effect is dependent on the number of fatty acid molecules bound to the protein while the chemical structure of fatty acids contained in the solution plays a minor role.
Department of Physical Pharmacy, Faculty of Pharmacy, Medical University of Silesia, 41-200 Sosnowiec, Poland. bbojko@sum.edu.pl
B
B
Bojko
A
A
Sułkowska
M
M
Maciazek-Jurczyk
J
J
Równicka
W W
WW
Sułkowski
D001665:Binding Sites; D003920:Diabetes Mellitus / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005247:Feeding Behavior; D006801:Humans; D007700:Kinetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D009765:Obesity / Q000378:metabolism*; D011485:Protein Binding; D012015:Reference Standards; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013806:Theophylline / Q000378:metabolism*
D005227:Fatty Acids; D012709:Serum Albumin; D013806:Theophylline