Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration.
Adipose tissue consists of mature adipocytes, preadipocytes and mesenchymal stem cells (MSCs), but a culture system for analyzing their cell types within the tissue has not been established. We have recently developed "adipose tissue-organotypic culture system" that maintains unilocular structure, proliferative ability and functions of mature adipocytes for a long term, using three-dimensional collagen gel culture of the tissue fragments. In this system, both preadipocytes and MSCs regenerate actively at the peripheral zone of the fragments. Our method will open up a new way for studying both multiple cell types within adipose tissue and the cell-based mechanisms of obesity and metabolic syndrome. Thus, it seems to be a promising model for investigating adipose tissue biology and regeneration. In this article, we introduce adipose tissue-organotypic culture, and propose two theories regarding the mechanism of tissue regeneration that occurs specifically at peripheral zone of tissue fragments in vitro.
Toda
Shuji
S
Department of Pathology and Biodefense University Hospital; Faculty of Medicine; Saga University; Nabeshima, Saga Japan.
Uchihashi
Kazuyoshi
K
Aoki
Shigehisa
S
Sonoda
Emiko
E
Yamasaki
Fumio
F
Piao
Meihua
M
Ootani
Akifumi
A
Yonemitsu
Nobuhisa
N
Sugihara
Hajime
H