Induction of oncogene addiction shift to NF-kappaB by camptothecin in solid tumor cells.
The biological basis of the resistance of solid tumor cells to chemotherapy is not well understood. While addressing this problem, we found that gastric cancer cell line St-4/CPT, lung cancer cell line A549/CPT, and colon cancer cell line HT-29/CPT, all of which are resistant to camptothecin (CPT), showed strong and constitutive nuclear factor (NF)-kappaB activity driven by IkappaB kinase compared with their parental cell lines St-4, A549, and HT-29. A new NF-kappaB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), reduced viability and induced apoptosis in St-4/CPT, A549/CPT, and HT-29/CPT cell lines, while their parental cell lines were resistant to DHMEQ. The results in this study present an example of the shift in signals that support the survival of solid tumor cells to NF-kappaB during the acquisition of resistance to CPT. The results also indicate that solid tumor cells that become resistant to chemotherapy may be more easily treated by NF-kappaB inhibitors.
Togano
Tomiteru
T
Department of Hematology, School of Medicine, Kitasato University, Sagamihara, Kanagawa 228-8555, Japan.
Sasaki
Masataka
M
Watanabe
Mariko
M
Nakashima
Makoto
M
Tsuruo
Takashi
T
Umezawa
Kazuo
K
Higashihara
Masaaki
M
Watanabe
Toshiki
T
Horie
Ryouichi
R
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis; D001549:Benzamides / Q000494:pharmacology; D002166:Camptothecin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003512:Cyclohexanones / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D009857:Oncogenes
D000972:Antineoplastic Agents, Phytogenic; D001549:Benzamides; D003512:Cyclohexanones; D016328:NF-kappa B; C464444:dehydroxymethylepoxyquinomicin; D002166:Camptothecin