[Effects of medicinal insect, Catharsius molossus on biosynthesis of polysaccharides from Ganoderma lucidum in submerged culture].
We studied the effects of several medicinal insects on biosynthesis of polysaccharides from Ganoderma lucidum in submerged culture. The results showed that the medicinal insect, Catharsius molossus at 5 g/L significantly promoted the biosynthesis of intracellular polysaccharides (IPS) and extracellular polysaccharides (EPS) of G. lucidum, and compared with control, IPS and EPS yields markedly enhanced from (1.93 +/- 0.09) g/L to (2.41 +/- 0.12) g/L and (520.3 +/- 20.2) mg/L to (608.9 +/- 20.2) mg/L, respectively (P < 0.05). Both IPS and EPS consisted of five kinds of components, and IPS-1 and EPS-1 were the major components of IPS and EPS, respectively. Further separation studies showed that IPS-1 was made up of three single compounds, while EPS-1 was made up of two single compounds. There were no new components in both IPS and EPS obtained from G lucidum in submerged culture by the addition of the insect, C. molossus, suggesting the biosynthetic pathways of the major components of IPS and EPS had not been changed.
Liu
Gaoqiang
G
College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China. gaoliuedu@yahoo.com.cn
Ding
Chongyang
C
Zhang
Kechang
K
Wang
Xiaoling
X
Han
Wenjun
W
D000818:Animals; D003058:Cockroaches / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D008419:Materia Medica / Q000494:pharmacology*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D020940:Reishi / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D008419:Materia Medica; D011134:Polysaccharides