Geographic HIV type 1 subtype distribution in Rakai district, Uganda.
To analyze HIV-1 subtype distribution, sequence analysis was performed on serum specimens obtained in 1994 from the Rakai Health Sciences community cohort in Uganda. Portions of gag-p24 and env-gp41 were sequenced and HIV subtype was determined for 773 subjects residing in 10 community clusters in rural Uganda. Subtypes A (17%) and D (70%) were the most common strains in the population. Subtype distribution varied by geographic region with significantly more subtype A in northern community clusters compared with southern clusters (21% vs. 8%, p < 0.001) and more subtype D in southern clusters compared with northern clusters (78% vs. 65%, p < 0.008). These data illustrate the geographic complexity of subtype variation, which has important implications for HIV-1 vaccine design.
Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Baltimore, MD 21205, USA.
Aleisha N
AN
Collinson-Streng
Andrew D
AD
Redd
Nelson K
NK
Sewankambo
David
D
Serwadda
Mona
M
Rezapour
Susanna L
SL
Lamers
Ronald H
RH
Gray
Maria J
MJ
Wawer
Thomas C
TC
Quinn
Oliver
O
Laeyendecker
D000293:Adolescent; D000328:Adult; D000818:Animals; D005260:Female; D005838:Genotype; D005843:Geography; D015700:HIV Envelope Protein gp41 / Q000235:genetics; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012424:Rural Population; D017422:Sequence Analysis, DNA; D014454:Uganda / Q000453:epidemiology; D055815:Young Adult; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D015700:HIV Envelope Protein gp41; D054301:gag Gene Products, Human Immunodeficiency Virus