Evaluation of the tissue reaction to fast endodontic cement (CER) and Angelus MTA.
BACKGROUND
A new cement (CER; Cimento Endodôntico Rápido or fast endodontic cement) has been developed to improve handling properties. It is a formulation that has Portland cement in gel. However, there had not yet been any study evaluating its biologic properties. The purpose of this study was to evaluate the rat subcutaneous tissue response to CER and Angelus MTA.


METHODS
The materials were placed in polyethylene tubes and implanted into dorsal connective tissue of Wistar rats for 7, 30, and 60 days. The specimens were prepared to be stained with hematoxylin-eosin or von Kossa or not stained for polarized light. The presence of inflammation, predominant cell type, calcification, and thickness of fibrous connective tissue were recorded. Scores were defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25-125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as thin when thickness was <150 mum and thick at >150 mum. Necrosis and formation of calcification were both recorded.


RESULTS
Both materials Angelus MTA and CER caused moderate reactions at 7 days, which decreased with time. The response was similar to the control at 30 and 60 days with Angelus MTA and CER, characterized by organized connective tissue and presence of some chronic inflammatory cells. Mineralization and granulations birefringent to polarized light were observed with both materials.


CONCLUSIONS
It was possible to conclude that CER was biocompatible and stimulated mineralization.
Gomes-Filho
João Eduardo
JE
Department of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, São Paulo, Brazil. joao@foa.unesp.br
Rodrigues
Guilherme
G
Watanabe
Simone
S
Estrada Bernabé
Pedro Felício
PF
Lodi
Carolina Simonett
CS
Gomes
Alessandra Cristina
AC
Faria
Max Doulgas
MD
Domingos Dos Santos
Alailson
A
Silos Moraes
João Carlos
JC
D017607:Aluminum Compounds / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002114:Calcinosis / Q000473:pathology; D017610:Calcium Compounds / Q000737:chemistry*; D003738:Dental Cements / Q000737:chemistry*; D004338:Drug Combinations; D005355:Fibrosis; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D008422:Materials Testing; D008859:Microscopy, Polarization; D009336:Necrosis; D010087:Oxides / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D040521:Subcutaneous Tissue / Q000473:pathology; D013997:Time Factors
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate