Inhibition of proliferation and differentiation and promotion of apoptosis by cyclin L2 in mouse embryonic carcinoma P19 cells.
Cyclin L2 (CCNL2) is a novel member of the cyclin gene family. In a previous study, we demonstrated that CCNL2 expression was upregulated in ventricular septum tissues from patients with ventricular septal defect compared to healthy controls. In the present study, we established a stable CCNL2-overexpressing P19 cell line that can differentiate to myocardial cells when treated with 1% dimethyl sulfoxide (DMSO). Our data showed that stable CCNL2-overexpressing P19 cells were less differentiated after treatment with 1% DMSO and that expression of myocardial cell differentiation-related genes (such as cardiac actin, GATA4, Mef2C, Nkx2.5, and BNP) were reduced compared to vector-only transfected P19. Moreover, P19 cells overexpressing the CCNL2 gene had a reduced growth rate and a remarkably decreased S phase. We also found that these cells underwent apoptosis, as detected by two different apoptosis assays. The anti-apoptotic Bcl-2 protein was also downregulated in these cells. In addition, real-time PCR analysis revealed that expression of Wnt and beta-catenin was suppressed and GSK3beta was induced in the CCNL2-overexpressing P19 cells. These data suggest that overexpression of CCNL2 inhibited proliferation and differentiation of mouse embryonic carcinoma P19 cells and induced them to undergo apoptosis, possibly through the Wnt signal transduction pathway.
Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, No 300 Guangzhou Road, Nanjing 210029, PR China.
Lili
L
Zhuo
Jie
J
Gong
Rong
R
Yang
Yanhui
Y
Sheng
Lei
L
Zhou
Xiangqing
X
Kong
Kejiang
K
Cao
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D018236:Carcinoma, Embryonal / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016213:Cyclins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D015398:Signal Transduction; D051153:Wnt Proteins / Q000235:genetics*
C499078:Ccnl2 protein, mouse; D016213:Cyclins; D051153:Wnt Proteins