High expression of N-acetylglucosaminyltransferase V in mucinous tumors of the ovary.
N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes beta1-6 branching of N-acetylglucosamine (GlcNAc) on asparagine-linked oligosaccharides of cell proteins. The present study aimed to investigate GnT-V expression and its prognostic significance in epithelial ovarian cancer. GnT-V expression was studied by immunohistochemistry in 83 surgically resected ovarian cancers, and the staining intensity was evaluated. High GnT-V expression in cancer cells was found in 17 (20.5%) of the 83 cases, and was positively correlated with early FIGO staging. In the histological type, mucinous adenocarcinoma showed significantly strong immunostaining compared to the non-mucinous type (P<0.001). In 36 mucinous tumors, the GnT-V immunostaining score was significantly higher in cancer than in benign and borderline tumors (P<0.001). NOM-1, a human ovarian mucinous adenocarcinoma cell line, expressed strong GnT-V protein and swainsonine treatment suppressed beta1-6GlcNAc branching and reduced migration ability significantly (P<0.001). These results suggested that GnT-V might be involved in the malignant potential of mucinous ovarian cancer.
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Noriko
N
Takahashi
Eiko
E
Yamamoto
Kazuhiko
K
Ino
Eiji
E
Miyoshi
Tetsuro
T
Nagasaka
Hiroaki
H
Kajiyama
Kiyosumi
K
Shibata
Akihiro
A
Nawa
Fumitaka
F
Kikkawa
D018262:Adenocarcinoma, Clear Cell / Q000201:enzymology* / Q000556:secondary / Q000601:surgery; D002288:Adenocarcinoma, Mucinous / Q000201:enzymology* / Q000556:secondary / Q000601:surgery; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D018284:Cystadenocarcinoma, Serous / Q000201:enzymology* / Q000556:secondary / Q000601:surgery; D016889:Endometrial Neoplasms / Q000201:enzymology* / Q000556:secondary / Q000601:surgery; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D010835:Phytohemagglutinins / Q000378:metabolism; D011379:Prognosis; D014407:Tumor Cells, Cultured
D010835:Phytohemagglutinins; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase