Effect of liver hypothermic preservation: exploration and protection.
OBJECTIVE
The principal aim of conservation is to maintain the viability of grafts. This requires the addition of a cellular protector allowing better conservation of the graft. The aim of this work is to evaluate the effect of trimetazidine (TMZ) addition to Wistar rat livers conserved in Krebs-Henseleit solution, compared to the livers preserved only in Krebs-Henseleit solution (24 h at 4 degrees C).


METHODS
40 Wistar female rats divided into 5 groups were used: the first group consists of nonpreserved livers, the second consists of livers preserved only in the Krebs-Henseleit solution, and the other 3 groups consist of livers preserved in Krebs solution with different concentrations of TMZ added (16.5, 49.5 and 165 microg/ml).


RESULTS
The obtained results show an improvement in the state of the liver in the presence of a high concentration of TMZ, which approaches normal physiological conditions. We note a clear diminution of transaminase activities, as well as an amelioration in metabolic capacities of the liver if the mitochondrial esterase pathway is supported in Wistar rats by a reduction of histological injuries.


CONCLUSIONS
A TMZ concentration of 165 microg/ml clearly restored the metabolic capacities of the liver. Indeed, TMZ limited the appearance of necrotic areas and almost suppressed apoptotic cells.
Ben Ali
R
R
Unité d'Expérimentation Animale, Faculté de Médecine de Tunis, Tunis, Tunisie. ridha_benali @ voila.fr
Klouz
A
A
Elkadri
N
N
Kourda
N
N
Gaies
E
E
Ben Saîd
D
D
Lakhal
M
M
Belkahia
C
C
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D050377:Cold Ischemia / Q000379:methods*; D005260:Female; D005947:Glucose; D066298:In Vitro Techniques; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D016031:Liver Transplantation; D009682:Magnetic Resonance Spectroscopy; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D014292:Trimetazidine / Q000008:administration & dosage; D014325:Tromethamine
C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D014325:Tromethamine; D005947:Glucose; D014292:Trimetazidine