Cerebral amyloidosis: postmortem detection with human 7.0-T MR imaging system.
OBJECTIVE
To explore the ability of whole-body 7.0-T magnetic resonance (MR) imaging to depict differences in aspects of the cerebral cortex of postmortem human brain specimens with cerebral amyloid beta deposition in connection with Alzheimer disease (AD), Down syndrome, or sporadic or hereditary cerebral amyloid angiopathy (CAA) and control brain specimens lacking such deposition.


METHODS
This study was approved by the local institutional review board. In all cases, informed consent was obtained to perform autopsy and to use the tissues for research purposes. T2- and T2*-weighted MR imaging was performed in formalin-fixed samples of brain tissue from six subjects with AD changes, seven with CAA, and five subjects without immunohistochemical evidence of cerebral amyloid beta deposition. All MR images were visually assessed for hypointense foci in and inhomogeneity of the cortex. Sensitivity, specificity, and kappa values of these MR imaging features in the detection of histologic changes were calculated.


RESULTS
High-spatial-resolution 0.3 x 0.3 x 0.3-mm three-dimensional T2*-weighted images revealed hypointense foci, inhomogeneity of the cortex, or both in all specimens with brain amyloid beta deposition. These MR imaging features were observed in none of the control specimens.


CONCLUSIONS
The finding of postmortem susceptibility-weighted changes in the cerebral cortex of patients with cerebral amyloidosis with a human 7.0-T MR imaging system opens up the possibility of obtaining in vivo radiologic evidence of cerebral amyloid beta deposition.
Department of Radiology, Leiden University Medical Center, C3-Q, PO Box 9600, 2300 RC Leiden, The Netherlands. S.van_Rooden@lumc.nl
Sanneke
S
van Rooden
Marion L C
ML
Maat-Schieman
Rob J A
RJ
Nabuurs
Louise
L
van der Weerd
Sara
S
van Duijn
Sjoerd G
SG
van Duinen
Remco
R
Natté
Mark A
MA
van Buchem
Jeroen
J
van der Grond
D002102:Cadaver; D016657:Cerebral Amyloid Angiopathy / Q000209:etiology / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D012307:Risk Factors; D012680:Sensitivity and Specificity