[The effect of tunicamycin on Fas protein expression and its function in fibroblasts from hypertrophic scar and keloid].
OBJECTIVE
To detect the effect of tunicamycin on Fas protein expression and Fas monoclonal antibody (FasMcAb)-induced apoptosis of fibroblast from hypertrophic scar and keloid.


METHODS
The expression of Fas protein was detected by immunostaining in 5 cases of keloid, 5 cases of hypertrophic scar and 5 cases of normal skin as control. The fibroblasts were cultured and treated with tunicamycin. The Fas protein expression and the fibroblast apoptosis rate were assessed by Western Blot and flow cytometry.


RESULTS
It revealed that Fas protein was detectable in all the three groups. The Fas glycosylation level was highest in hypertrophic scar, but lowest in normal skin. The FasMcAb-induced apoptosis had a positive relationship with the Fas glycosylation. Tunicamycin had a significant inhibitory effect on the Fas glycosylation in keloid and hypertrophic scar, but not in normal skin.


CONCLUSIONS
The FasMcAb-induced apoptosis has a positive relationship with the Fas glycosylation. Tunicamycin has a significant inhibitory effect on the Fas glycosylation in keloid and hypertrophic scar.
Department of Plastic Surgery, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China.
Yong-Bo
YB
Liu
Jian-Hua
JH
Gao
Xiao-Jun
XJ
Liu
Feng
F
Lu
Hong-Wei
HW
Liu
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D008297:Male; D015398:Signal Transduction; D014415:Tunicamycin / Q000494:pharmacology*; D055815:Young Adult; D019014:fas Receptor / Q000378:metabolism*
D000911:Antibodies, Monoclonal; C491901:FAS protein, human; D019014:fas Receptor; D014415:Tunicamycin