The expression and significance of HIF-1alpha and GLUT-3 in glioma.
HIF-1alpha plays an indispensable role in tumor formation and histogenesis. Target genes involved in glucose transport are acutely transactivated by HIF-1alpha. GLUT-3 protein is the rate-limiting factor related to glucose transport, which is classified as brain-type glucose transporter. This study was the initial one aiming to probe into the co-expression and clinical significance of HIF-1alpha and GLUT-3 in glioma. One hundred and twenty cases of glioma tissues and ten human normal cerebral tissues decompressed in glioma excision were examined using immunohistochemistry and Western blot. The expression of HIF-1alpha and GLUT-3 increased gradually with the increase of pathological grade of glioma, respectively. There was significant difference in the expression of HIF-1alpha and GLUT-3 in every two groups, respectively. There was a positive correlation between HIF-1alpha and GLUT-3. In conclusion, the expression of HIF-1alpha and GLUT-3 in glioma was correlated significantly with tumors' pathological grade, which can be taken as a pair of useful markers for predicting the biological behavior of glioma.
Liu
Yang
Y
Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Xi'an, 710032, China.
Li
Yun-ming
YM
Tian
Rui-feng
RF
Liu
Wei-ping
WP
Fei
Zhou
Z
Long
Qian-fa
QF
Wang
Xiao-an
XA
Zhang
Xiang
X
D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism; D002648:Child; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D055815:Young Adult
D000199:Actins; D051274:Glucose Transporter Type 3; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C495687:SLC2A3 protein, human