Selective immobilization of oligonucleotide-modified gold nanoparticles by electrodeposition on screen-printed electrodes.
Here, we describe a proof of concept procedure for the selective immobilization of oligonucleotides functionalized gold nanoparticle probes (affinity modules) on arrayed screen-printed gold electrodes. Current microarrays are using many different ways to address their DNA probes onto the transducer area. For that reason, we have mixed the electrodeposition of metals, which is a very well known process, in addition with the DNA-gold nanoparticles formation, which is an area of great interest in biosensing applications in the field of genomics, clinical and warfare applications. Combining these fields, we have developed a novel method for the immobilization of gold nanoparticles conjugated with oligonucleotides (affinity modules) onto screenprinting gold electrodes through electrodeposition at a current positive potential of 800mV vs. Ag/AgCl. The modules were selectively immobilized onto the electrode surface being, afterwards, ready for a successful hybridization. The gold colloids take the advantage of being a carrier that allows the immobilization of any kind of bioreceptor in the same conditions and the capability of quality control analysis before the electrodeposition procedure. With this system, we avoided non-specific interactions between the transduction layer and the bioreceptor and in the case of DNA oligonucleotides allowed us the immobilization of multiple sequences in a multimodular device for a further industrial process of cheaper biochip fabrication.
Departamento Bioquímica y Biología Molecular, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain. mmoreno@bioapter.com
M
M
Moreno
E
E
Rincon
J M
JM
Pérez
V M
VM
González
A
A
Domingo
E
E
Dominguez
D015374:Biosensing Techniques / Q000295:instrumentation; D015342:DNA Probes / Q000737:chemistry* / Q000648:ultrastructure; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004595:Electroplating / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*
D015342:DNA Probes; D006046:Gold