Speeding up (13)C direct detection biomolecular NMR spectroscopy.
After the exploitation of (1)H polarization as a starting source for (13)C direct detection experiments, pulse sequences are designed which exploit the accelerated (1)H longitudinal relaxation to expedite (13)C direct detection experiments. We show here that 2D experiments based on (13)C direct detection on a 0.5 mM water sample of ubiquitin can be recorded in a few minutes and 3D experiments in a few hours. We also show that fast methods like nonuniform sampling can be easily implemented. As overall experimental time has always been a counter indication for the use of (13)C direct detection experiments, this research opens new avenues for the application of (13)C NMR to biological molecules.
Bruker BioSpin GmbH, Silberstreifen, 76287 Rheinstetten, Germany.
Wolfgang
W
Bermel
Ivano
I
Bertini
Isabella C
IC
Felli
Roberta
R
Pierattelli
D002247:Carbon Isotopes / Q000032:analysis*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017434:Protein Structure, Tertiary; D013997:Time Factors; D025801:Ubiquitin / Q000737:chemistry
D002247:Carbon Isotopes; D025801:Ubiquitin