[Effects of IFN alpha on the expressions of Collagen I and transforming growth factor beta 1 in hepatic stellate cell activated by PDGF-BB].
OBJECTIVE
To investigate the effect of IFN alpha on the expressions of Collagen I and TGF beta 1 in hepatic stellate cell activated by PDGF-BB.


METHODS
Hepatic stellate cells (rHSC-99) treated with IFN alpha of different concentration (0, 0.0125, 0.025, 0.050, 0.100, 0.200, 0.400 ng/ml). The cell viability of HSC was measured by MTT. The levels of Col-I mRNA and TGF beta 1 mRNA were measured by the quantitative reverse-transcription polymerase chain reaction (RT-PCR).


RESULTS
(1) When HSC was exposed in PDGF-BB, the cell viability of HSC (1.35 +/- 0.22) was higher than that of the control group (0.890 +/- 0.12) (F = 16.311, P less than 0.05), indicating that PDGF-BB can promote the cell viability of HSC. When HSC was exposed to both PDGF-BB and different concentration of IFN alpha (0.025, 0.05, 0.1, 0.2, 0.4 ng/ml), the cell viability of HSC (0.840 +/- 0.18, 0.450 +/- 0.15, 0.260 +/- 0.01, 0.330 +/- 0.07, 0.30 +/- 0.06) were lower than that of the control group (0.890 +/- 0.12) (F = 7.430, P less than 0.05), indicating that the cell viability of HSC was inhibited when HSC was exposed to both PDGF-BB and different concentrations of IFN alpha. Furthermore, within the range of 0.025 ng/ml to 0.1 ng/ml, the effect of IFN alpha was dose-dependent. (2). The relative expression values of Col-I mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (0.940 +/- 0.19, 0.610 +/- 0.12, 0.520 +/- 0.02), which were lower than those in the control group (1.410 +/- 0.01) (F = 127.921, P less than 0.05). The relative expression values of TGF beta 1 mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (1.180 +/- 0.06, 1.150 +/- 0.10, 1.390 +/- 0.04), again were lower than those in the control group (1.620 +/- 0.12) (F = 82.115, P less than 0.05). These results indicated that the expression of Col-I mRNA and TGF beta 1 mRNA was remarkably inhibited when HSC was exposed in both PDGF-BB and IFN alpha.


CONCLUSIONS
The cell viability of HSC and the expression of Col-I mRNA and TGF beta 1 mRNA is remarkably inhibited when HSC is exposed in both PDGF-BB and IFN alpha, and the inhibition is dose-dependent.
Department of Infectious Diseases, Affiliated Shengjing Hospital of China Medical University, Shenyang 110003, China.
Jing
J
Hu
Zhi-wei
ZW
Li
Xiu-jin
XJ
Yang
Yue-jie
YJ
Yang
Li
L
Ma
D000818:Animals; D000077214:Becaplermin; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism*; D016898:Interferon-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
D024042:Collagen Type I; D016898:Interferon-alpha; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin