The redox status of experimental hemorrhagic shock as measured by cyclic voltammetry.
Hemorrhagic shock (HS) leads to reactive oxygen species production. However, clinicians do not have access to bedside measurements of the redox status during HS. Cyclic voltammetry (CyV) is a simple electrochemical method of measuring redox status. The aims of this study were to 1) report the first application of cyclic voltammetry to measure the acute changes in serum redox status after HS, 2) to contrast it with another severe systemic disease with a different redox pathology (acute pancreatitis [AP]), and 3) to describe the response of CyV over time in a resolving model of AP. In the acute study, 24 male Wistar rats were randomized into three groups: groups 1 (control), 2 (AP), and 3 (HS). In the time-course study, 28 rats were randomized to a sham-control as well as 6 and 24 h post-AP cohorts, respectively.Cyclic voltammetry was performed using a three-electrode system. In the acute study, the first and second voltammetric peaks increased significantly in HS. In contrast, within the AP group, only the first voltammetric peak showed a significant increase. The first voltammetric peak correlated with plasma protein carbonyls (PCs) and with thiobarbituric acid-reactive substances, whereas the second voltammetric peak correlated positively with plasma protein carbonyls. In the second study, the first voltammetric peak correlated with physiological improvements. Here, we showed that serum CyV could respond to the serum redox change in HS and AP. Cyclic voltammetry warrants evaluation as a potential real-time beside measure of a patient's redox status during shock.
Mittal
Anubhav
A
Department of Surgery, Faculty of Medicine and Health Sciences, University of Auckland, New Zealand.
Göke
Friederike
F
Flint
Richard
R
Loveday
Benjamin P T
BP
Thompson
Nichola
N
Delahunt
Brett
B
Kilmartin
Paul A
PA
Cooper
Garth J S
GJ
MacDonald
Julia
J
Hickey
Anthony
A
Windsor
John A
JA
Phillips
Anthony R J
AR
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004195:Disease Models, Animal; D055664:Electrochemical Techniques / Q000379:methods*; D008297:Male; D010084:Oxidation-Reduction; D010195:Pancreatitis / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000097:blood*
D000975:Antioxidants