The adverse effect of phytoestrogens on the synthesis and secretion of ovarian oxytocin in cattle.
The current investigations were undertaken to study the mechanism of the adverse effect of phytoestrogens on the function of bovine granulosa (follicles >1< cm in diameter) and luteal cells from day 1-5, 6-10, 11-15, 16-19 of the oestrous cycle. The cells were incubated with genistein, daidzein or coumestrol (each at the dose of 1 × 10(-6) m). The viability and secretion of estradiol (E2), progesterone (P4) and oxytocin (OT) were measured after 72 h of incubation. Moreover, the expression of mRNA for neurophysin-I/OT (NP-I/OT; precursor of OT) and peptidyl-glycine-α-amidating monooxygenase (PGA, an enzyme responsible for post-translational OT synthesis) was determined after 8 h of treatment. None of the phytoestrogens used affected the viability of cells except for coumestrol. The increased secretion of E2 and P4 was only obtained by coumestrol (p<0.05) from granulosa cells from follicles <1cm in diameter and decreased from luteal cells on days 11-15 of the oestrous cycle, respectively. All three phytoestrogens stimulated (p<0.05) OT secretion from granulosa and luteal cells in all stages of the oestrous cycle and the expression of NP-I/OT mRNA in the both types of cells. The expression of mRNA for PGA was stimulated (p<0.05) by daidzein and coumestrol in granulosa cells, and by genistein and coumestrol in luteal cells. In conclusion, our results demonstrate that these phytoestrogens can impair the ovary function in cattle by adversely affecting the synthesis of OT in follicles and in corpus luteum. However, their influence on the ovarian steroids secretion was less evident.
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.
J
J
Mlynarczuk
M H
MH
Wrobel
J
J
Kotwica
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D003375:Coumestrol / Q000494:pharmacology; D005260:Female; D019833:Genistein / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics; D009097:Multienzyme Complexes / Q000235:genetics; D009481:Neurophysins / Q000235:genetics; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D010121:Oxytocin / Q000096:biosynthesis* / Q000378:metabolism*; D048789:Phytoestrogens / Q000009:adverse effects*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007529:Isoflavones; D009097:Multienzyme Complexes; D009481:Neurophysins; D048789:Phytoestrogens; D012333:RNA, Messenger; D010121:Oxytocin; C004742:daidzein; D019833:Genistein; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D003375:Coumestrol