Anti-inflammatory effects of YeongyoSeungma-tang.
BACKGROUND
YeongyoSeungma-tang which includes Fructus Forsythia, has been used in oriental traditional medicine for treatment of early smallpox and atopic dermatitis.


OBJECTIVE
YeongyoSeungma-tang was carried out to investigate for anti-inflammatory effects.


METHODS
YeongyoSeungma-tang was tested for anti-inflammatory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E(2) (PGE(2)), and tumor necrosis factor alpha (TNF-alpha) releases as well as nuclear factor kappa B (NF-kappaB) expression using RAW264.7 macrophage cells.


RESULTS
YeongyoSeungma-tang significantly inhibited generation of NO (42% and 59% inhibition at doses of 5 microg/mL and 10 microg/mL, respectively), PGE(2) (46% and 80% inhibition at doses of 5 microg/mL and 10 microg/mL, respectively) and TNF-alpha (6% and 23% inhibition at doses of 5 microg/mL and 10 microg/mL, respectively) on LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. Consistently in these observations, the expression of inducible NO synthase (iNOS) enzyme was also inhibited by YeongyoSeungma-tang. However, YeongyoSeungma-tang did not show any influence on the expression of cyclooxygenase-2. The cream containing 0.075% YeongyoSeungma-tang showed good skin moisturizing effect without any irritation.


CONCLUSIONS
The present study may support the fact that YeongyoSeungma-tang can have the good possibility as an anti-inflammatory agent for troubled skins.
R&D Center, Saimdang Cosmetics Co., Ltd., 493-1 Daeheung-dong, Jung-gu, Daejeon, Republic of Korea.
Mi Jin
MJ
Kim
Kyung Ran
KR
Im
Kyung-Sup
KS
Yoon
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D015232:Dinoprostone