Stretching the timescale of intravital imaging in tumors.
Since the time it was pioneered in 1992, intravital imaging of tumors at cellular resolution has offered us the extremely important opportunity of "seeing biology." However, until now, most studies were monitoring tumor cell behavior in the same animal over short times, requiring the combining of acquired data into a hypothesis via statistical analysis. In the last year, different groups have independently developed techniques to extend the time scale of intravital imaging to several days. This improvement allows one to address the connection between tumor cell behavior and the microenvironment which surrounds them. We can now assess dynamics of the cell-cell interactions in tumors, analyze tumor cell fate and changes in the tumor extracellular matrix which accompany tumor progression.
Department of Anatomy and Structural Biology, Gruss-Lipper Biophotonic Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA. bgligori@aecom.yu.edu
Bojana
B
Gligorijevic
John
J
Condeelis
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D002450:Cell Communication; D002465:Cell Movement; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D024022:Fibrillar Collagens / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D049452:Green Fluorescent Proteins; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D009361:Neoplasm Invasiveness / Q000473:pathology; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000473:pathology; D017154:Stromal Cells / Q000473:pathology; D013997:Time Factors
D024022:Fibrillar Collagens; D049452:Green Fluorescent Proteins