Improving the detection of hydrogen peroxide of screen-printed carbon paste electrodes by modifying with nonionic surfactants.
Nonionic surfactants, such as Triton X-100 and Tween-20, were shown in this study to improve the electrocatalytic activity of screen-printed carbon paste electrodes (SPCE). The electrochemical response of SPCE to hydrogen peroxide increased 8-10-fold with the modification of nonionic surfactants. In addition, the glucose biosensors fabricated from nonionic surfactant-modified SPCE exhibited 6.4-8.6-fold higher response to glucose than that fabricated from unmodified SPCE. A concentration effect is proposed for nonionic surfactant to bring neutral reactants to the surface of electrode. Moreover, nonionic surfactant-modified SPCE exhibits a capability of repetitive usage and good reproducibility (R.S.D.<5%) in the measurement of H(2)O(2). Interestingly, the nonionic surfactant-modified SPCE exhibited an opposite effect to ascorbic acid, a common electroactive agent, which causes interference during clinical diagnosis. The differential responses of nonionic surfactant-modified SPCE to H(2)O(2) and ascorbic acid suggest its potential in the development of biosensors for clinical diagnosis.
Department of Biological Science and Technology, National Chiao Tung University, 75 Bo Ai Street, Hsinchu 300, Taiwan, ROC. cjyuan@mail.nctu.edu.tw
Chiun-Jye
CJ
Yuan
Yen-Chi
YC
Wang
Ohara
O
Reiko
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D013501:Surface-Active Agents / Q000737:chemistry*
D004800:Enzymes, Immobilized; D013501:Surface-Active Agents; D002244:Carbon; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose