Toxicity of hexachloronaphthalene (HxCN) and induction of CYP 1A in rats.
The aim of the study was to investigate the toxicity of hexachloronaphthalene (HxCN) and its effect on cytochrome P-450 in rats and to make a comparison between HxCN and tetrachloronaphthalene (TeCN), an inactive congener. Our study provided evidence that the anorectic effect, with concurrent significant increase in relative liver mass was the most spectacular symptom of the toxic effect of hexachloronaphthalene in the rats after its single (250mg/kg) and repeated (1 and 10mg/kg) administration. Regardless of the kind of the experiment (acute or subacute toxicity), dose-dependent increase in lipid peroxidation in the liver was also observed, which may indicate that HxCN most probably generates oxidative stress in this organ. It was also observed that HxCN is a very strong inducer of cytochrome P-450, especially of CYP 1A, which is the most sensitive biomarker of exposure to this congener. In this study, LOAEL is 1mg HxCN/kgb.w.
Kilanowicz
Anna
A
Department of Toxicology, Faculty of Pharmacy, Medical University, Muszynskiego 1, 90-151 Lodz, Poland. anna.kilanowicz@umed.lodz.pl
Skrzypinska-Gawrysiak
Malgorzata
M
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009281:Naphthalenes / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D020033:Protein Isoforms / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D009281:Naphthalenes; D020033:Protein Isoforms; D008315:Malondialdehyde; C049022:1,2,3,4,5,6-hexachloronaphthalene; D019363:Cytochrome P-450 CYP1A1; D005978:Glutathione