Immobilization of DNA on microporous PVDF membranes by plasma polymerization.
Microporous poly(vinylidene fluoride) (PVDF) membranes with different porous surface morphologies were prepared by immersion-precipitation from coagulation baths of different strengths. On these membranes single-strand deoxyribonucleic acid (ss-DNA) was covalently immobilized by a dual-step procedure. First, poly(glycidyl methacrylate) (PGMA) was grafted on PVDF membranes by plasma-induced free radical polymerization. Then, ss-DNA was bonded to PGMA through ring-opening reactions of epoxies with amine to form amino alcohols. The highest attainable graft yield of PGMA on PVDF membrane was 0.3 mg/cm(2), which was the case when a highly porous PVDF membrane was employed as the substrate. For immobilization of ss-DNA, the yield was found to depend significantly on the reaction temperature and pH. The maximal value was 48.5 mug/cm(2). Furthermore, adsorption tests of anti-DNA antibody were carried out on membranes with and without immobilized ss-DNA using serum obtained from systemic lupus erythematosus patients. The results indicated that the immobilized DNA could effectively adsorb the antibody in the serum.
Lin
Dar-Jong
DJ
Department of Chemical and Materials Engineering, Tamkang University, Taipei 25137, Taiwan.
Lin
Dong-Tsamn
DT
Young
Tai-Horng
TH
Chen
Tsung-Chin
TC
Chang
Hsu-Hsien
HH
Cheng
Liao-Ping
LP
D000327:Adsorption; D001128:Argon / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D013696:Temperature; D005465:Fluorocarbon Polymers
D001128:Argon; D004277:DNA, Single-Stranded; D008567:Membranes, Artificial; D011109:Polymethacrylic Acids; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C042535:polyglycidyl methacrylate