Distribution of Mycoplasma haemofelis in blood and tissues following experimental infection.
The aim of the study was to describe blood and tissue copy number distribution during Mycoplasma haemofelis infection and determine if sequestration of organisms in body tissues could explain blood copy number cycling in infected cats. Thirteen domestic-shorthaired cats were used. Blood samples were regularly collected, and at a differing time point post-infection for each cat, tissue samples also collected, for quantitative PCR (qPCR). Absolute haemoplasma copy numbers were calculated for all blood and tissue samples, as well as an estimation of the ratio of tissue haemoplasma copy number to that expected in the tissue if a positive qPCR result arose due to tissue blood supply alone. Cats with high or moderate M. haemofelis blood copy numbers at the time of tissue collection had fewer M. haemofelis copies in most tissues than expected due to the tissue blood supply alone; only splenic and lung tissues consistently contained more M. haemofelis. However tissues collected from cats at a time of very low M. haemofelis blood copy numbers, when putative copy number cycling nadirs were occurring, were usually qPCR negative. Hence no evidence of significant tissue M. haemofelis sequestration was found in this study to explain the copy number cycling reported with this feline haemoplasma species.
University of Bristol, Langford, United Kingdom. s.tasker@bristol.ac.uk
Séverine
S
Tasker
Iain R
IR
Peters
Michael J
MJ
Day
Barbara
B
Willi
Regina
R
Hofmann-Lehmann
Timothy J
TJ
Gruffydd-Jones
Chris R
CR
Helps
D000818:Animals; D016470:Bacteremia / Q000382:microbiology / Q000662:veterinary*; D002371:Cat Diseases / Q000097:blood / Q000382:microbiology*; D002415:Cats; D004269:DNA, Bacterial / Q000097:blood / Q000302:isolation & purification; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D008297:Male; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
D004269:DNA, Bacterial