RhoB enhances migration and MMP1 expression of prostate cancer DU145.
Rho family protein regulates variety of cellular functions as cytoskeletal organization, cell proliferation and apoptosis. In the present study, we demonstrate that RhoB-overexpressed prostate cancer cells showed an enhanced cell motility and the administration of the GSK-3 inhibitors inhibited this increase in migration. Among the extracellular matrix and adhesion-related molecules, MMP1 RNA expression was increased in RhoB-overexpressed cells, administration of MMP inhibitor suppressed the collagen gel invasion in these cells. This is the first report evaluating RhoB function and the downstream signaling events in prostate cancer cell. Our results indicate that RhoB promotes cell motility and invasion in a metastatic prostate cancer cell.
Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20 Minami, Daiko, Higashi-ku, Nagoya City, Aichi, Japan.
Misao
M
Yoneda
Yoshifumi S
YS
Hirokawa
Atsuyuki
A
Ohashi
Katsunori
K
Uchida
Daisuke
D
Kami
Masatoshi
M
Watanabe
Toyoharu
T
Yokoi
Taizo
T
Shiraishi
Shinya
S
Wakusawa
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D020743:rhoB GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D038362:Glycogen Synthase Kinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D020743:rhoB GTP-Binding Protein