A novel method for the functionalization of gamma-irradiated single wall carbon nanotubes with DNA.
In this work we describe a novel method for highly efficient functionalization of single wall carbon nanotubes (SWCNTs) by DNA wrapping. Exposure of SWCNTs to gamma-irradiation (50 kGy) has lowered by one order of magnitude the amount of single stranded deoxyribonucleic acid (ssDNA) required for SWCNT modification. The resulting hybrids of gamma-irradiated SWCNTs and ssDNA were characterized by optical absorbance spectroscopy, Raman spectroscopy and Fourier transform infrared spectroscopy. Atomic force microscopy was used to investigate the morphology of hybrids. While gamma-irradiation in three different media has significantly improved the process of SWCNT dispersion, irradiation in ammonia was the most efficient. The gamma-irradiated SWCNTs functionalized with ssDNA were stabilized by electrostatic forces. This preliminary study suggests that gamma-irradiation can significantly improve the functionalization of SWCNTs with DNA.
Vinca Institute of Nuclear Sciences, POB 522, 11001 Belgrade, Serbia. svetlanajovanovic@vinca.rs
S P
SP
Jovanović
Z M
ZM
Marković
D N
DN
Kleut
N Z
NZ
Romcević
V S
VS
Trajković
M D
MD
Dramićanin
B M
BM
Todorović Marković
D000388:Air; D000641:Ammonia / Q000737:chemistry; D000818:Animals; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005720:Gamma Rays; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000528:radiation effects*; D009994:Osmolar Concentration; D012474:Salmon; D012965:Sodium Chloride / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D055672:Static Electricity; D014867:Water / Q000737:chemistry
D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; D014867:Water; D012965:Sodium Chloride; D000641:Ammonia