Antibacterial activity of essential oil components and their potential use in seed disinfection.
Among the main (> or = 0.7%) components of some essential oils, considerable antibacterial activity was shown by terpenoid and phenylpropanoid derivatives containing phenol and alcohol functionalities. A reduced or no activity was shown by those derivatives containing ketones, aldehydes, ethers, and ester functionalities as well as the remaining terpenoids. Eugenol emulsion treatments (1-8 mg/mL) of bean seeds bearing about 2.6 x 10(6) cfu/seed of strain ICMP239 of Xanthomonas campestris pv. phaseoli var. fuscans determined a highly significant reduction of the bacteria on seeds. In particular, eugenol at 4 mg/mL disinfect seeds bearing about 7.0 x 10(2) cfu/seed and lower densities. However, after 72 h, incubation treatments with 2, 4, and 8 mg/mL of eugenol caused germination reduction of 3%, 7%, and 16%, respectively, which was significantly different from the controls. No effect on germination was observed with 1 mg/mL eugenol emulsion treatment. These data indicate eugenol as potentially useful for bean seed disinfection from X. campestris pv. phaseoli var. fuscans. Further studies on the effects on seed vitality and on formulation of essential oils are needed.
Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Universita degli Studi della Basilicata, Viale dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
Pietro
P
Lo Cantore
Vellasamy
V
Shanmugaiah
Nicola Sante
NS
Iacobellis
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D004202:Disinfectants / Q000494:pharmacology*; D005054:Eugenol / Q000494:pharmacology; D007887:Fabaceae / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology; D018525:Germination / Q000187:drug effects; D009822:Oils, Volatile / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004202:Disinfectants; D009822:Oils, Volatile; D005054:Eugenol