BchY-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR.
To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA. yutin@ncbi.nlm.nih.gov
Natalya
N
Yutin
Marcelino T
MT
Suzuki
Mira
M
Rosenberg
Denisse
D
Rotem
Michael T
MT
Madigan
Jörg
J
Süling
Johannes F
JF
Imhoff
Oded
O
Béjà
D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D008969:Molecular Sequence Data; D010788:Photosynthesis; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment
D001426:Bacterial Proteins; D017931:DNA Primers