Spectrofluorimetric quantification of bromazepam using a highly selective optical probe based on Eu3+-bromazepam complex in pharmaceutical and serum samples.
A rapid, simple and sensitive spectrofluorimetric method for determination of trace amount of bromazepam is developed. In phosphate buffer of pH 7.4. The bromazepam enhance the luminescence intensity of the Eu(3+) ion in Eu(3+)-bromazepam complex at lambda(ex)=390nm. The produced luminescence intensity of Eu(3+)-bromazepam complex is in proportion to the concentration of bromazepam. The working range for the determination of bromazepam is 2.3x10(-8) to 6.2x10(-7)M with detection limit (LoD) and quantitative detection limit (LoQ) of 3x10(-9) and 1.2x10(-8)M, respectively. While, the working range, detection limit (LoD) and quantitative detection limit (LoQ) in case of the quantum yield calculations are 3.7x10(-8) to 3.4x10(-7)M with of 3.4x10(-9) and 9.2x10(-8)M, respectively. The enhancement mechanism of the luminescence intensity in the Eu(3+)-bromazepam system has been also explained.
Attia
M S
MS
Department of Chemistry, Faculty of Science, Ain Shams University, Cairo 115611, Egypt. Mohamed sam@yahoo.com
D014151:Anti-Anxiety Agents / Q000032:analysis* / Q000097:blood; D001960:Bromazepam / Q000032:analysis* / Q000097:blood; D005063:Europium / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000191:economics / Q000379:methods*; D013997:Time Factors
D014151:Anti-Anxiety Agents; D005063:Europium; D001960:Bromazepam