Validation of a real-time PCR for Haemophilus parasuis.
OBJECTIVE
To validate a real-time PCR test for the diagnosis of Glässer's disease, a major pig disease caused by Haemophilus parasuis.


RESULTS
The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples of DNA from tissues and fluids of 16 experimentally challenged animals were tested with the real-time PCR, and the results were compared with culture and a conventional PCR. The real-time PCR produced significantly more positive results than the conventional PCR (165 vs 86).


CONCLUSIONS
The sensitivity of the real-time PCR combined with high specificity makes it a very valuable tool for the diagnosis of Glässer's disease.


CONCLUSIONS
This new method will improve the ability of laboratories to diagnose Glässer's disease, especially in laboratories where the culture method for H. parasuis is not optimal.
Animal Research Institute, Queensland Primary Industries and Fisheries, Yeerongpilly, Qld, Australia. Conny.Turni@deedi.qld.gov.au
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Turni
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Pyke
P J
PJ
Blackall
D000818:Animals; D006192:Haemophilus Infections / Q000175:diagnosis / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*