The role of keratinocyte-derived chemokine in hemorrhage-induced acute lung injury in mice.
Dominant inflammatory cytokines might be different depending on the underlying causes of acute lung injury (ALI). The role of kertinocyte-derived chemokine (KC), a potent chemoattractant for neutrophils, has not been clearly established in hemorrhage-induced ALI. In this study, lung injury and cytokine expression were evaluated in LPS- or hemorrhage-induced ALI models of BALB/c mice. The myeloperoxidase activities at 4 hr after hemorrhage and LPS-injection were 47.4+/-13.0 and 56.5+/-16.4 U/g, respectively. NF-kappaB activity peaked at 4 hr after hemorrhage, which was suppressed to the control level by anti-high mobility group B1 (HMGB1) antibody. Lung expressions of TNF-alpha, MIP-2, and IL-1beta were increased by LPS injection. However, there was only a minimal increase in IL-1beta and no expressions of TNF-alpha or MIP-2 in hemorrhage-induced ALI. In contrast, lung KC increased significantly at 4 hr after hemorrhage compared to control levels (83.1+/-12.3 vs. 14.2+/-1.6 pg/mL/mg by ELISA) (P<0.05). By immunohistochemical staining, lung neutrophils stained positive for KC. Increased KC was also observed in bronchoalveolar lavage fluid and plasma. KC plays an important role in hemorrhage-induced ALI.
Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
Byoung Hoon
BH
Lee
Tae Jin
TJ
Lee
Jae Woo
JW
Jung
Dong Jin
DJ
Oh
Jae Chol
JC
Choi
Jong Wook
JW
Shin
In Won
IW
Park
Byoung Whui
BW
Choi
Jae Yeol
JY
Kim
D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D054426:Chemokine CXCL2 / Q000032:analysis; D018925:Chemokines / Q000032:analysis / Q000097:blood / Q000502:physiology*; D002645:Chickens; D024243:HMGB1 Protein / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D009195:Peroxidase / Q000032:analysis; D012771:Shock, Hemorrhagic / Q000150:complications*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D000906:Antibodies; D054426:Chemokine CXCL2; D018925:Chemokines; D024243:HMGB1 Protein; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C074302:keratinocyte-derived chemokines; D009195:Peroxidase