A study on the perfusion preservation, resuscitation, and transplantation of a rat heart isolated for 96 hours.
Krebs-Henseleit (KH) solution was used to fill the heart chamber of an isolated rat heart before it was immersed in perfluorocarbon (PFC), which is an inert fluid. A gas mixture (PCO(2) = 150 hPa and PO(2) = 850 hPa) was then aerated at a constant rate into the PFC solution, and the isolated heart was thereafter preserved for 96 h with KH solution perfused continuously at a rate of 0.1 ml/h from the aorta of the isolated heart through a cannula. After preservation, the preserved heart was heterotopically transplanted into the neck of a recipient rat and then it was resuscitated. Using this method for preserving mammalian organs, we attained reproducibility after perfusion preservation for 96 h.
Hatayama
Naoyuki
N
Kanagawa University Faculty of Science, Hiratsukashi, 259-1293, Japan. nao_h416@yahoo.co.jp
Yoshida
Yu
Y
Seki
Kunihiro
K
D000818:Animals; D005466:Fluorocarbons / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006321:Heart / Q000502:physiology*; D016027:Heart Transplantation; D008297:Male; D015425:Myocardial Reperfusion; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012151:Resuscitation; D013997:Time Factors; D014325:Tromethamine / Q000494:pharmacology
D005466:Fluorocarbons; C074097:Krebs-Henseleit solution; D014325:Tromethamine; D005947:Glucose