FGF4 independent derivation of trophoblast stem cells from the common vole.
The derivation of stable multipotent trophoblast stem (TS) cell lines from preimplantation, and early postimplantation mouse embryos has been reported previously. FGF4, and its receptor FGFR2, have been identified as embryonic signaling factors responsible for the maintenance of the undifferentiated state of multipotent TS cells. Here we report the derivation of stable TS-like cell lines from the vole M. rossiaemeridionalis, in the absence of FGF4 and heparin. Vole TS-like cells are similar to murine TS cells with respect to their morphology, transcription factor gene expression and differentiation in vitro into derivatives of the trophectoderm lineage, and with respect to their ability to invade and erode host tissues, forming haemorrhagic tumours after subcutaneous injection into nude mice. Moreover, vole TS-like cells carry an inactive paternal X chromosome, indicating that they have undergone imprinted X inactivation, which is characteristic of the trophoblast lineage. Our results indicate that an alternative signaling pathway may be responsible for the establishment and stable proliferation of vole TS-like cells.
Grigor'eva
Elena V
EV
Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Department, Novosibirsk, Russia.
Shevchenko
Alexander I
AI
Mazurok
Nina A
NA
Elisaphenko
Eugeny A
EA
Zhelezova
Antonina I
AI
Shilov
Alexander G
AG
Dyban
Pavel A
PA
Dyban
Andrey P
AP
Noniashvili
Ekaterina M
EM
Slobodyanyuk
Sergey Ya
SY
Nesterova
Tatyana B
TB
Brockdorff
Neil
N
Zakian
Suren M
SM
D000818:Animals; D003411:Arvicolinae; D001755:Blastocyst / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D051519:Fibroblast Growth Factor 4 / Q000378:metabolism*; D005786:Gene Expression Regulation; D006493:Heparin / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D011003:Ploidies; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*; D014157:Transcription Factors / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology*
D051519:Fibroblast Growth Factor 4; D014157:Transcription Factors; D006493:Heparin