Analysis of meiotic recombination in Caenorhabditis elegans.
Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. A variety of techniques have been developed for the measurement of meiotic recombination in C. elegans, ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.
Hillers
Kenneth J
KJ
Biological Sciences, California Polytechnic State University, San Luis Obispo, CA, USA.
Villeneuve
Anne M
AM
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D005819:Genetic Markers / Q000502:physiology; D005821:Genetic Techniques; D008540:Meiosis / Q000235:genetics*; D008957:Models, Genetic; D009866:Oogenesis / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D011839:Radiation, Ionizing; D011995:Recombination, Genetic / Q000235:genetics* / Q000528:radiation effects; D013091:Spermatogenesis / Q000235:genetics
D005819:Genetic Markers