Thymidine production by overexpressing NAD+ kinase in an Escherichia coli recombinant strain.
Abstract Intracellular NADPH/NADP+ ratio in cells grown on various production media with different carbon and nitrogen sources had a positive correlation with the thymidine production. To improve thymidine production in a previously engineered E. coli strain, NAD+ kinase was overexpressed in it resulting in the NADPH/NADP+ ratio shifting from 0.184 to 0.267. The [NADH + NADP+]/[NAD+ + NADPH] ratio was, however, not significantly altered. In jar fermentation, 740 mg thymidine l-1 was produced in parental strain, while 940 mg l-1 of thymidine was produced in NAD+ kinase-expressing strain.
BioNgene Co., Ltd, 10-1, 1Ka, Myungryun-Dong, Jongro-Ku, Seoul 110-521, Republic of Korea. churry@biongene.com
Hyeon Cheol
HC
Lee
Jin Sook
JS
Kim
Wonhee
W
Jang
Sang Yong
SY
Kim
D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D018628:Gene Dosage; D009584:Nitrogen / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013936:Thymidine / Q000096:biosynthesis*
D003470:Culture Media; D011994:Recombinant Proteins; D002244:Carbon; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase; D009584:Nitrogen; D013936:Thymidine