Enantioseparations on amylose tris(5-chloro-2-methylphenylcarbamate) in nano-liquid chromatography and capillary electrochromatography.
Amylose tris(5-chloro-2-methylphenylcarbamate) was coated onto native and aminopropylsilanized silica in order to prepare chiral stationary phases (CSP) for enantioseparations using nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC). The effect of the nature of silica, the particle size and pore diameter, the chiral selector loading onto silica, the mobile phase composition and pH, as well as separation variables such as a linear flow rate of the mobile phase, applied voltage in CEC, etc. on the separation of enantiomers was studied. It was found that CSPs based on amylose tris(5-chloro-2-methylphenylcarbamate) can be used for preparation of stable capillary columns for enantioseparations by nano-LC and CEC in combination with polar organic and aqueous-organic mobile phases. Higher peak efficiency was observed in CEC than in nano-LC.
Institute of Chemical Methodologies, Consiglio Nazionale delle Ricerche, Area della Ricerca di Roma I, Via Salaria Km 29, 300-00015 Monterotondo Scalo (Roma), Italy.
Salvatore
S
Fanali
Giovanni
G
D'Orazio
Ketevan
K
Lomsadze
Shorena
S
Samakashvili
Bezhan
B
Chankvetadze
D000688:Amylose / Q000031:analogs & derivatives*; D053801:Capillary Electrochromatography / Q000379:methods*; D002219:Carbamates / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D053841:Electroosmosis; D044950:Flavanones / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism
D002219:Carbamates; D044950:Flavanones; D012822:Silicon Dioxide; D000688:Amylose