Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID.
Bacillus subtilis fmbJ (CGMCC no.0943) was used to produce lipopeptides antibiotics fengycin. The fengycin mixture was precipitated with 6N HCl and extracted by methanol. Electrospray ionization mass spectrometry/collision-induced dissociation (ESI-MS/CID) were used to analyze the fengycin mixture. In the ESI-MS/CID spectra of fengycin molecular ions, production ions (m/z 1080, m/z 966, m/z 1108 and m/z 994) were always detected. Therefore, these production ions (m/z 1080, m/z 966, m/z 1108 and m/z 994) could be used as fingerprints to quickly detect fengycin A and fengycin B. By the method of ESI-MS/CID, six homologues of fengycin A with C14 to 19 and four homologues of fengycin B with C14 to 17 were characterized. The method will be useful for rapidly determining whether a strain produces fengycin homologues.
College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang Nanjing 210095, PR China.
Xiaomei
X
Bie
Zhaoxin
Z
Lu
Fengxia
F
Lu
D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055666:Lipopeptides / Q000737:chemistry* / Q000378:metabolism; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D055666:Lipopeptides; C049972:fengycin