[In vitro study on the effect of ribavirin reducing the EV71 replication].
OBJECTIVE
Observe the effect of Ribavirin on reducing the EV71 replication, inactivating EV71 and protecting the RD-A cells against the EV71 infection in vitro.


METHODS
Using the EV71 isolated from Anhui Fuyang HFMD outbreak, the effect of Ribavirin on RD-A cells during the EV71 infection was observed.


RESULTS
In the experiment of Ribavirin inhibiting the EV71 replication, comparing with the no-Ribavirin-dealed virus control group, the group of 1:640 dilution of Ribavirin delayed the CPE for 2 days, while the normal cell group was growing very well. In the experiment of protecting cell from EV71 infection, comparing with the no-Ribavirin-dealed virus control group, the group of 1:8 dilution of Ribavirin delayed the CPE for 4 days. In the experiment of Ribavirin effect on the inactivation of EV71, the group of 1:40 dilution of Ribavirin delayed the CPE for 2 days comparing with the virus control group.


CONCLUSIONS
Ribavirin could inhibit the replication of the EV71 and inactivate the EV71 in vitro. Additionally, Ribavirin could protect RD-A cells from EV 71 infection. The observation will contribute to EV71 infection control and quick medicine therapy.
National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100050, China.
Yan
Y
Zhang
Zhen
Z
Zhu
Shuang-Li
SL
Zhu
Hong-Qiu
HQ
An
Zhao-Hui
ZH
Yang
Xue-Bin
XB
Guo
Wen-Bo
WB
Xu
D002460:Cell Line; D004770:Enterovirus / Q000187:drug effects* / Q000502:physiology*; D004769:Enterovirus Infections / Q000821:virology*; D006801:Humans; D012254:Ribavirin / Q000494:pharmacology*; D038301:Virus Inactivation / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
D012254:Ribavirin