SOD and CAT cDNA cloning, and expression pattern of detoxification genes in the freshwater bivalve Unio tumidus transplanted into the Moselle river.
The cDNA sequences encoding manganese superoxide dismutase (Mn-SOD) and catalase (CAT) were isolated in the freshwater bivalve Unio tumidus by reverse-transcription polymerase chain reaction (RT-PCR) using degenerate primers. Quantitative real-time PCR approach was used to evaluate the mRNA expression patterns of SOD, CAT, selenium-dependent glutathione peroxidase (Se-GPx), pi class glutathione S-transferase (pi-GST) and metallothionein (MT), in the digestive gland of Unio tumidus transplanted from a control site to four stations in the Moselle River (M1-M4), for periods of 8 and 21 days. These sites were chosen upstream and downstream of populated areas. Chemical analysis performed on sediments from the Moselle river sites did not show high levels of pollutants. Decrease of SOD, CAT, Se-GPx and MT mRNA levels were observed at M3 site after a 21-day exposure compared to control site. These results suggest inefficiency of antioxidant systems affected by cytotoxic mechanisms and confirm an environmental perturbation. Organisms transplanted at M4 site showed a strong increase of biomarkers transcription levels after 21 days of exposure. These inductions could correspond to an adaptive response to an altered environment. Our results showed that biological approaches using multibiomarkers appear as essential tools complementary to measurement of contaminants, to detect environmental degradations.
Lab. L.I.E.B.E, CNRS UMR 7146, Université de Metz, Rue Delestraint, 57070, Metz, France.
Aurélie
A
Bigot
Paule
P
Vasseur
François
F
Rodius
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002374:Catalase / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005602:France; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics; D008658:Inactivation, Metabolic / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics; D008670:Metals / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045483:Rivers; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic; D049881:Unio / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D018076:DNA, Complementary; D008670:Metals; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase