Evaluation of the measurement of serum cystatin C by an enzyme-linked immunosorbent assay for humans as a marker of the glomerular filtration rate in dogs.
The serum cystatin C (Cys-C) concentration is a better filtration marker than plasma creatinine (Cre) concentration in humans. In veterinary medicine, a few studies have shown that the serum Cys-C concentration in dogs is also a better marker than the plasma Cre concentration. The purpose of this study is to evaluate the applicability of measuring the serum Cys-C concentration by an enzyme-linked immunosorbent assay (ELISA) as a marker of the glomerular filtration rate in dogs with various renal dysfunctions. The serum Cys-C concentration in dogs with chronic kidney disease (CKD) was significantly higher (1.23 +/- 0.21 mg/L) than that in 76 control dogs (0.85 +/- 0.15) (P<0.001). The reference range of the serum Cys-C concentrations in samples from the 76 control dogs was 0.55-1.15 mg/l. Serum Cys-C concentration was more strongly correlated with plasma iohexol clearance (r=-0.704, P<0.001) than plasma Cre concentration in dogs (r=-0.598, P<0.001). In a receiver operating characteristics analysis, significant differences between the serum Cys-C and plasma Cre concentrations were found with regard to their AUC (0.949, [SE, 0.019] and 0.849 [SE, 0.029]) and diagnostic sensitivity (90.3% and 73.6%) for detecting decreased PCio (P<0.05). Therefore, the measurement of serum Cys-C concentration by ELISA is more useful for the detection of early CKD than measuring the plasma Cre concentration.
Laboratory of Veterinary Internal Medicine, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino City, Tokyo, Japan. d0607@nvlu.ac.jp
Yuichi
Y
Miyagawa
Naoyuki
N
Takemura
Hisashi
H
Hirose
D000818:Animals; D015415:Biomarkers / Q000097:blood; D055316:Cystatin C / Q000097:blood*; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005919:Glomerular Filtration Rate / Q000662:veterinary*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000662:veterinary*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; D055316:Cystatin C