Generation of induced pluripotent stem cells from human cord blood.
Induced pluripotent stem cells (iPSCs) may represent an ideal cell source for future regenerative therapies. A critical issue concerning the clinical use of patient-specific iPSCs is the accumulation of mutations in somatic (stem) cells over an organism's lifetime. Acquired somatic mutations are passed onto iPSCs during reprogramming and may be associated with loss of cellular functions and cancer formation. Here we report the generation of human iPSCs from cord blood (CB) as a juvenescent cell source. CBiPSCs show characteristics typical of embryonic stem cells and can be differentiated into derivatives of all three germ layers, including functional cardiomyocytes. For future therapeutic production of autologous and allogeneic iPSC derivatives, CB could be routinely harvested for public and commercial CB banks without any donor risk. CB could readily become available for pediatric patients and, in particular, for newborns with genetic diseases or congenital malformations.
Haase
Alexandra
A
Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Hannover, Germany.
Olmer
Ruth
R
Schwanke
Kristin
K
Wunderlich
Stephanie
S
Merkert
Sylvia
S
Hess
Christian
C
Zweigerdt
Robert
R
Gruh
Ina
I
Meyer
Johann
J
Wagner
Stefan
S
Maier
Lars S
LS
Han
Dong Wook
DW
Glage
Silke
S
Miller
Konstantin
K
Fischer
Philipp
P
Schöler
Hans R
HR
Martin
Ulrich
U
D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction