[Retrospective diagnosis of congenital CMV infection in DBS from Guthrie cards: French experience].
Systematic screening for cytomegalovirus congenital infection is not performed in France. For children with hearing loss or other neurological CMV compatible symptoms, retrospective diagnosis is possible by PCR detection of CMV DNA in dried blood spot of neonatal Guthrie cards. We report here the results obtained with this technique in the French national reference laboratory for cytomegalovirus.
Leruez-Ville
M
M
Laboratoire de virologie, centre national de référence du cytomégalovirus-laboratoire associé, hôpital Necker-Enfants-Malades, 149 rue de Sèvres, Paris, France. marianne.leruez@nck.aphp.fr
Vauloup-Fellous
C
C
Couderc
S
S
Parat
S
S
Ouchérif
S
S
Castel
C
C
Magny
J-F
JF
D001800:Blood Specimen Collection; D003587:Cytomegalovirus / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000175:diagnosis / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005312:Fetal Blood / Q000821:virology*; D005602:France; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015997:Neonatal Screening; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D013048:Specimen Handling; D014766:Viremia / Q000151:congenital / Q000175:diagnosis / Q000821:virology
D004279:DNA, Viral