[Effect of early enteral immune nutrition on immune function of intestine in mice with severe burn].
OBJECTIVE
To investigate the effects of early enteral nutrition supplemented with immune nutrient on intestine immune function in mice with severe burn.


METHODS
Twenty-four BALB/c mice were inflicted with 20% TBSA full-thickness scald, then they were randomly divided into EN(with oral administration of common enteral nutrition after 2 hours) and EIN (with oral administration of common enteral nutrition and glutamine, arginine after 2 hours) groups. Another 10 mice were used as the normal control (NC) group. The supplied energy ratio( carbohydrate: fat: protein)in former 2 groups was 82:3:15, and the ratio of energy to nitrogen was 150: 1. The energy requirement of each mouse was calculated according to 732.2 kJ x kg(-1) x d(-1), one third of the requirement was administrated on 1st day, and one half of it on 2nd day, and full energy requirement was started on the 3rd day,and the requirement was divided into 4-6 portions every day. The feed was isocaloric, isonitrogenous, and isovolumic for the 2 experimental groups. All mice were sacrificed and entire small intestine was harvested for determination of intestinal IgA level by ELISA, total Peyer's patches (PP) lymphocytes and their apoptosis ratio, and changes in PP lymphocytes (CD3+, CD4+, CD19+) on 7th day of the experiment.


RESULTS
Compared with those in NC group [(4.5 +/- 0.6) x 10(6), (42 +/- 7) microg/cm, respectively], total PP lymphocytes and intestinal IgA levels in EN and EIN groups obviously decreased [(2.3 +/- 0.4) x 10(6), (35 +/- 6) microg/cm, (3.8 +/- 0. 5) x 10(6), (38 +/- 6), microg/cm, respectively, P < 0.05] , among which the values in EIN group were higher than EN group (P < 0.05). The changes in PP lymphocytes were similar to that of total PP lymphocytes. Compared with that in NC group [(4.8 +/- 2.1)%], the apoptosis ratio of PP lymphocytes in EN and EIN groups significantly increased [(12.7 +/- 2.4)%, (8.0 +/- 1.7)%, respectively, P < 0.05], however the ratio in EIN group was lower than that of EN group (P < 0.05).


CONCLUSIONS
Early enteral nutrition supplemented with immune nutrient can improve intestinal immune function in mice with severe burn.
Department of Burns, the General Hospital of Shenyang Military Command, Shenyang 110015, China.
Jun
J
Fan
Qing-Yan
QY
Meng
Guang-Hua
GH
Guo
Yong
Y
Xie
Yue
Y
Li
Fu-Sun
FS
Hu
Yi-Ping
YP
Xiu
Tai-Ran
TR
Li
Liang
L
Ma
D000818:Animals; D002056:Burns / Q000276:immunology* / Q000503:physiopathology / Q000628:therapy*; D004750:Enteral Nutrition; D007421:Intestine, Small / Q000276:immunology; D007422:Intestines / Q000276:immunology*; D016131:Lymphocyte Subsets; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C