[Determination of optical purity of alpha-phenylethylamine by high performance liquid chromatography with pre-column derivatization].
A simple pre-column derivatization-high performance liquid chromatographic (HPLC) method was established for the determination of optical purity of alpha-phenylethylamine. The enantiomers of alpha-phenylethylamine were derivatized with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (GITC). The resulted diastereoisomers were separated on an Agilent Zorbax C18 column (250 mm x 4.6 mm, 5 microm) with a mobile phase of methanol-phosphate buffer (1.36 g/L aqueous solution of potassium dihydrogen phosphate, adjusted to pH 3.0 with concentrated phosphoric acid) (58:42, v/v). The flow rate was set at 1.0 mL/min and the detection wavelength was set at 241 nm. The method was linear from 0.15 - 15.0 mg/L for both enantiomers. The limit of detection and the limit of quantification were 0.05 mg/L and 0.15 mg/L, respectively. The relative standard deviations (RSDs) of inter- and intra-day determination were below 0.5%. The method is easy to handle, accurate, and suitable for the quality control of the optical purity of alpha-phenylethylamine.
Wang
Jinzhao
J
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Zeng
Su
S
Wang
Danhua
D
Hu
Gongyun
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010627:Phenethylamines / Q000032:analysis* / Q000737:chemistry*; D010777:Photochemistry; D013237:Stereoisomerism
D010627:Phenethylamines; C033198:1-phenethylamine