[MTT assay for detecting osteosarcoma cell apoptosis].
OBJECTIVE
To assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.


METHODS
The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.


RESULTS
MTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.


CONCLUSIONS
MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.
Chen
Qiang
Q
Department of Spinal and Orthopedic Surgery, Southern Medical University, Guangzhou, China. cqnfyy@yahoo.com.cn
Zhang
Jin-hua
JH
Chen
Jian-ting
JT
Shi
Zhan-jun
ZJ
Deng
Yong-jian
YJ
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000473:pathology* / Q000601:surgery; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology* / Q000601:surgery; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue