Analysis of the interaction of the nucleotide base with myosin and the effect on substrate efficacy.
A wide variety of purine- and pyrimidine-based nucleotides can serve as a substrate for actomyosin mechanics, but with varying effectiveness. To understand the myosin-ATP interaction and in particular, the interactions with the base, we have used molecular dynamics simulations to model the interactions of myosin with ATP, CTP, UTP, aza-ATP, ITP, and GTP (in decreasing order of effectiveness as a substrate for the generation of motility) docked at the active site. The simulations with ATP, and x-ray structures, show a triad of conserved amino acids lining the nucleotide site that form a cyclical chain of nucleotide-protein hydrogen bonding interactions: ATP --> Y135 --> Y116 --> N188 --> ATP. Mechanical efficacy of a substrate correlates with its ability to maintain this coordination. Simulations modeling the active site of other myosin isoforms with different amino acids in the triad likewise imply that the amino acid composition at the nucleotide site could modulate function. The modeling has predictive power. In silico mutation experiments suggest mutations that would enhance GTP as a substrate for myosin while simultaneously making ATP a less effective substrate.
Department of Mathematics, Washington State University, Pullman, Washington, USA.
David
D
Hyatt
Roger
R
Cooke
Edward
E
Pate
D000205:Actomyosin / Q000378:metabolism; D020134:Catalytic Domain; D004023:Dictyostelium; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D009154:Mutation; D009218:Myosins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009711:Nucleotides / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism
D009711:Nucleotides; D015800:Protozoan Proteins; D000205:Actomyosin; D009218:Myosins