Effect of liver hypothermic preservation: exploration and protection.
OBJECTIVE
The principal aim of conservation is to maintain the viability of grafts. This requires the addition of a cellular protector allowing better conservation of the graft. The aim of this work is to evaluate the effect of trimetazidine (TMZ) addition to Wistar rat livers conserved in Krebs-Henseleit solution, compared to the livers preserved only in Krebs-Henseleit solution (24 h at 4 degrees C).


METHODS
40 Wistar female rats divided into 5 groups were used: the first group consists of nonpreserved livers, the second consists of livers preserved only in the Krebs-Henseleit solution, and the other 3 groups consist of livers preserved in Krebs solution with different concentrations of TMZ added (16.5, 49.5 and 165 microg/ml).


RESULTS
The obtained results show an improvement in the state of the liver in the presence of a high concentration of TMZ, which approaches normal physiological conditions. We note a clear diminution of transaminase activities, as well as an amelioration in metabolic capacities of the liver if the mitochondrial esterase pathway is supported in Wistar rats by a reduction of histological injuries.


CONCLUSIONS
A TMZ concentration of 165 microg/ml clearly restored the metabolic capacities of the liver. Indeed, TMZ limited the appearance of necrotic areas and almost suppressed apoptotic cells.
Unité d'Expérimentation Animale, Faculté de Médecine de Tunis, Tunis, Tunisie. ridha_benali @ voila.fr
R
R
Ben Ali
A
A
Klouz
N
N
Elkadri
N
N
Kourda
E
E
Gaies
D
D
Ben Saîd
M
M
Lakhal
C
C
Belkahia
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D050377:Cold Ischemia / Q000379:methods*; D005260:Female; D005947:Glucose; D066298:In Vitro Techniques; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D016031:Liver Transplantation; D009682:Magnetic Resonance Spectroscopy; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D014292:Trimetazidine / Q000008:administration & dosage; D014325:Tromethamine
C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D014325:Tromethamine; D005947:Glucose; D014292:Trimetazidine