Comparision of biocompatibility and cytotoxicity of two new root canal sealers.
The aim of this study was to investigate the remote organ toxicity and connective tissue reaction of two new root canal sealers ("GuttaFlow(®)" and "EndoREZ(®)") and to compare them with zinc oxide eugenol sealer using biochemical and histopathological parameters. A total of 60 white albino Wistar rats were used in the study. 0.1ml of GuttaFlow(®), EndoREZ(®) or Kerr Pulp Canal Sealer(®) were administered subcutaneously into the mid-dorsal thoracic region of rats (15 in each group). Control rats were given saline only. Rats were decapitated after 24h, on day 7 and on day 30 of the experiment and tissue samples from lung, liver, kidney and skin were removed for the determination of malondialdehyde (MDA) and glutathione (GSH) levels. In parallel, tissues were also examined histologically. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, and creatinine and blood urea nitrogen (BUN), concentrations (BUN) were measured to assess liver and kidney functions, respectively. Tumor necrosis factor (TNF) and lactate dehydrogenase (LDH) were also assayed in serum samples. No statistical differences were found among the control and EndoREZ(®), GuttaFlow(®) and Kerr Pulp Canal sealers regarding tissue MDA, GSH levels or serum parameters (p>0.05) at all time points examined. Both of the new root canal sealers showed good compatibility and acceptable tissue toxicity.
Department of Endodontics, Faculty of Dentistry, Marmara University, Nişantaşı, Istanbul, Turkey.
Nimet
N
Gencoglu
Göksel
G
Sener
Gulden Z
GZ
Omurtag
Ayfer
A
Tozan
Bahar
B
Uslu
Serap
S
Arbak
Dilek
D
Helvacioglu
D000818:Animals; D003188:Composite Resins / Q000008:administration & dosage / Q000009:adverse effects / Q000633:toxicity*; D004129:Dimethylpolysiloxanes / Q000008:administration & dosage / Q000009:adverse effects / Q000633:toxicity*; D004338:Drug Combinations; D005978:Glutathione / Q000032:analysis; D006171:Gutta-Percha / Q000009:adverse effects / Q000633:toxicity*; D007279:Injections, Subcutaneous; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000009:adverse effects / Q000633:toxicity*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D015035:Zinc Oxide-Eugenol Cement / Q000008:administration & dosage / Q000009:adverse effects / Q000633:toxicity*
D003188:Composite Resins; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; C481259:EndoRez; C515718:GuttaFlow; D012387:Root Canal Filling Materials; D014409:Tumor Necrosis Factor-alpha; D015035:Zinc Oxide-Eugenol Cement; D008315:Malondialdehyde; D006171:Gutta-Percha; D007770:L-Lactate Dehydrogenase; D005978:Glutathione