GATEWAY technology and E. coli recombinant system produce a properly folded and functional recombinant allergen of the lipid transfer protein of apple (Mal d 3).
The lipid transfer protein of apple fruit, Mal d 3, has been produced as a soluble recombinant protein in transformed Escherichia coli cells using the GATEWAY technology. Circular dichroism spectra showing the protein essentially consists of alpha-helices indicate that the rMal d 3 is properly folded. The (1)H NMR spectra also indicates a correct fold for the recombinant allergen. The reactivity of rMal d 3 towards IgE from apple allergic patients and in vitro degranulation activity measured on transformed rat basophil leukemia cells expressing the human Fc epsilon RI alpha-subunit of rMal d 3 is similar to those of the native allergen purified from apple fruits. The expression of active rMal d 3 in E. coli is readily feasible and offers an interesting alternative to the production of recombinant allergen in the yeast Pichia pastoris. This expression in E. coli open the way to the modification of Mal d 3 by site-directed mutagenesis for immunotherapy purposes.
Borges
Jean-Philippe
JP
Surfaces Cellulaires et Signalisation chez les Végétaux, UMR UPS-CNRS 5546, Université de Toulouse, 24 Chemin de Borde Rouge, 31326 Castanet, Tolosan, France. joao.borges@fc.up.pt
Culerrier
Raphaël
R
Aldon
Didier
D
Barre
Annick
A
Benoist
Hervé
H
Saurel
Olivier
O
Milon
Alain
A
Didier
Alain
A
Rougé
Pierre
P
D000485:Allergens / Q000235:genetics*; D000818:Animals; D052179:Antigens, Plant; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D027845:Malus / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
D000485:Allergens; D052179:Antigens, Plant; D002352:Carrier Proteins; C463180:Mal d 3 allergen; D011994:Recombinant Proteins; C030363:lipid transfer protein