Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces.
In vitro studies of the initial attachment, spreading and motility of human bone mesenchymal stem cells have been carried out on bovine osteopontin (OPN) coated hydroxyapatite (HA) and gold (Au) model surfaces. The adsorption of OPN extracted from bovine milk was monitored by the quartz crystal microbalance with dissipation (QCM-D) and the ellipsometry techniques, and the OPN coated surfaces were further investigated by antigen-antibody interaction. It is shown that the OPN surface mass density is significantly lower and that the number of antibodies binding to the resulting OPN layers is significantly higher on the HA as compared to the Au surfaces. The initial attachment, spreading and motility of human mesenchymal stem cells show a larger cell area, a faster arrangement of vinculin in the basal cell membrane and more motile cells on the OPN coated HA surfaces as compared to the OPN coated Au surfaces and to the uncoated Au and HA surfaces. These in vitro results indicate that there may be great potential for OPN coated biomaterials, for instance as functional protein coatings or drug delivery systems on orthopaedic implants or scaffolds for tissue-engineering.
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C, Denmark.
Thomas
T
Jensen
Alireza
A
Dolatshahi-Pirouz
Morten
M
Foss
Jørgen
J
Baas
Jette
J
Lovmand
Mogens
M
Duch
Finn Skou
FS
Pedersen
Moustapha
M
Kassem
Cody
C
Bünger
Kjeld
K
Søballe
Flemming
F
Besenbacher
D000327:Adsorption / Q000187:drug effects; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003460:Crystallization; D017886:Durapatite / Q000494:pharmacology*; D005453:Fluorescence; D006046:Gold / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D053495:Osteopontin / Q000494:pharmacology*; D011791:Quartz; D013194:Staining and Labeling; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D016596:Vinculin / Q000378:metabolism
D000906:Antibodies; D020099:Coated Materials, Biocompatible; D053495:Osteopontin; D016596:Vinculin; D011791:Quartz; D006046:Gold; D017886:Durapatite