Fabrication of a novel impedance cell sensor based on the polystyrene/polyaniline/Au nanocomposite.
Gold nanoparticles (AuNPs) were assembled on the surface of polystyrene (PS) and polyaniline (PANI) core-shell nanocomposite (PS@PANI) for the immobilization of HL-60 leukemia cells to fabricate a cell electrochemical sensor. The immobilized cells exhibited irreversible voltammetric response and increased the electron transfer resistance with a good correlation to the logarithmic value of concentration ranging from 1.6 x 10(3) to 1.6 x 10(8) cells mL(-1) with a limit of detection of 7.3 x 10(2) cells mL(-1) at 10 sigma. This biosensor was simple, low cost and disposable, which implied that the PS@PANI/Au composites can regard as the potential applications for clinical applications.
Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Miaomiao
M
Gu
Jingjing
J
Zhang
Ye
Y
Li
Liping
L
Jiang
Jun-Jie
JJ
Zhu
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D018914:Cells, Immobilized; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011137:Polystyrenes / Q000737:chemistry*
D000814:Aniline Compounds; D011137:Polystyrenes; C416807:polyaniline; D006046:Gold