Association of PDE4D and IL-1 gene polymorphism with ischemic stroke in a Han Chinese population.
BACKGROUND
The single-nucleotide polymorphisms (SNPs) of the phosphodiesterase 4D (PDE4D) and interleukin-1 (IL-1) genes are associated with increased risk for the development of ischemic stroke (IS) in whites. However, little is known about whether this association could also occur in Han Chinese.


METHODS
A total of 371 patients with IS and unrelated healthy controls were recruited and the SNPs of the PDE4D (83T/C), (87T/C), IL-1 (-889C/T) and IL-1 (-511C/T) were characterized, respectively, by polymerase chain reactions-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of these SNPs in this population were statistically analyzed.


RESULTS
The genotype and allele frequencies of the PDE4D (87T/C) and IL-1 (-511C/T) were similar between IS patients and controls. In contrast, the frequencies of CC genotype and C allele of the PDE4D (83T/C) and the T allele frequency of IL-1 (-889C/T) in IS patients were significantly higher than that in healthy controls (p=0.001, p=0.003 and p=0.02, respectively), independent of the conventional risk factors. The values of odds ratio (OR) reached at OR=1.603; 95%CI=1.032-2.489; p=0.036 for the CC genotype of the PDE4D (83T/C) and OR=1.913; 95%CI=1.621-2.375; p=0.034 for the TT genotype of the IL-1 (-889C/T), respectively.


CONCLUSIONS
the SNPs of the PDE4D (83T/C) and IL-1 (-889C/T) were associated with increased risk for the development of IS in Northern Han Chinese.
Li
Nan
N
Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China.
He
Zhiyi
Z
Xu
Jialiang
J
Liu
Fang
F
Deng
Shumin
S
Zhang
Hui
H
D044466:Asian People / Q000235:genetics; D002545:Brain Ischemia / Q000235:genetics*; D002681:China; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3 / Q000235:genetics*; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D020521:Stroke / Q000235:genetics*
D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; C517376:PDE4D protein, human