Influence of the tissue microenvironment on Toll-like receptor expression by CD11c+ antigen-presenting cells isolated from mucosal tissues.
It is recognized that functional activities of antigen-presenting cells (APCs) in mucosal tissue sites differ from those of systemic APCs; however, it is unknown whether there are further differences between APC populations residing in different mucosal sites. In this study, we directly compared murine CD11c+ APCs isolated from colon, lung, and spleen and found that APCs isolated from these tissues differ considerably in Toll-like receptor (TLR) expression and responses to in vitro TLR ligand stimulation. We also provide evidence that tissue microenvironments dictate distinct patterns of TLR expression by CD11c+ APCs in different mucosal tissues. Moreover, CD11c+ cells isolated from different tissues have varied capacities to induce the development of T helper 1 (Th1), Th2, or regulatory CD4+ T cells. Thus, unique tissue microenvironments have a significant influence on determining TLR expression by CD11c+ cells that migrate to and reside in each mucosal tissue and are likely to modulate their functional activities.
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
Shunsuke
S
Takenaka
Sarah
S
McCormick
Ekaterina
E
Safroneeva
Zhou
Z
Xing
Jack
J
Gauldie
D000818:Animals; D000938:Antigen-Presenting Cells / Q000737:chemistry* / Q000276:immunology*; D039521:CD11c Antigen / Q000032:analysis*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003106:Colon / Q000276:immunology; D020869:Gene Expression Profiling; D008168:Lung / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D051193:Toll-Like Receptors / Q000096:biosynthesis*
D039521:CD11c Antigen; D051193:Toll-Like Receptors