Protein kinase C is involved in the regulation of several calreticulin posttranslational modifications.
Calreticulin (CRT) is a highly versatile lectin-like chaperone that affects many cellular functions both inside and outside the endoplasmic reticulum lumen. We previously reported that calreticulin interacts with several protein kinase C isozymes both in vitro and in vivo. The aim of this study was to elucidate the molecular determinants involved in the association between these proteins and the biochemical significance of their interaction. Using full-length or CRT-domain constructs expressed as GST-fusion proteins, we found that protein kinase C binds to the CRT N domain in overlay and pull-down assays. Phosphorylation experiments showed that only this CRT domain is phosphorylated by the kinase. Lectin blot analysis demonstrated that CRT is modified by N-glycosylation, but this modification did not affect its interaction with protein kinase C. We also demonstrated that although both domains of protein kinase C theta can bind to CRT, it is the catalytic one that binds with higher affinity to CRT. Immunofluorescence studies showed that CRT and PKC co-localize mainly at the ER (estimated in 35%). Activation of protein kinase C induced caused transient changes in CRT localization, and unexpectedly, also induced changes in posttranslational modifications found in the protein: CRT N-glycosylation is abolished, whereas tyrosine phosphorylation and O-linked beta-N-acetylglucosamine modification are increased. Together, these findings suggest that protein kinase C is involved in the regulation of CRT function.
Department of Biochemistry, Universidad Nacional Autónoma de México (UNAM), Mexico D.F. 04510, Mexico.
M
M
Cristina Castañeda-Patlán
Roberto
R
Razo-Paredes
Rolando
R
Carrisoza-Gaytán
Lorenza
L
González-Mariscal
Martha
M
Robles-Flores
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D037282:Calreticulin / Q000737:chemistry / Q000378:metabolism*; D020134:Catalytic Domain; D002522:Chlorocebus aethiops; D004789:Enzyme Activation; D006031:Glycosylation; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D011817:Rabbits; D051381:Rats
D037282:Calreticulin; D011493:Protein Kinase C