High-fat diet and hydrochlorothiazide increase oxidative stress in brain of rats.
This study evaluated the effect of possible synergic interaction between high fat diet (HF) and hydrochlorothiazide (HCTZ) on biochemical parameters of oxidative stress in brain. Rats were fed for 16 weeks with a control diet or with an HF, both supplemented with different doses of HCTZ (0.4, 1.0, and 4.0 g kg(-1) of diet). HF associated with HCTZ caused a significant increase in lipid peroxidation and blood glucose levels. In addition, HF ingestion was associated with an increase in cerebral lipid peroxidation, vitamin C and non-protein thiol groups (NPSH) levels. There was an increase in vitamin C as well as NPSH levels in HCTZ (1.0 and 4.0 g kg(-1) of diet) and HF plus HCTZ groups. Na(+)-K(+)-ATPase activity of HCTZ (4.0 g kg(-1) of diet) and HCTZ plus HF-fed animals was significantly inhibited. Our data indicate that chronic intake of a high dose of HCTZ (4 g kg(-1) of diet) or HF change biochemical indexes of oxidative stress in rat brain. Furthermore, high-fat diets consumption and HCTZ treatment have interactive effects on brain, showing that a long-term intake of high-fat diets can aggravate the toxicity of HCTZ.
Hospital Universitário de Santa Maria, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. mcpribeiro@yahoo.com.br
Marinei Cristina Pereira
MC
Ribeiro
Nilda Berenice de Vargas
NB
Barbosa
Tielle Moraes
TM
de Almeida
Lutiane Mozzaquatro
LM
Parcianello
Juliano
J
Perottoni
Daiana Silva
DS
de Avila
João Batista Teixeira
JB
Rocha
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology*; D006852:Hydrochlorothiazide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001786:Blood Glucose; D004041:Dietary Fats; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D006852:Hydrochlorothiazide; D000254:Sodium-Potassium-Exchanging ATPase; D001205:Ascorbic Acid