[Evaluation of chromogenic media in microbiological diagnostics].
The aim of the study was the evaluation ofchromogenic agars as a screening media in the routine microbiological diagnostics. 627 clinical samples were cultured on the chromogenic media: chromID S. aureus, chromID MRSA, chromID CPS3 and chromID ESBL from bioMerieux. The results of presumptive identification and detection of selected resistance mechanisms by chromogenic media were compared with results obtained with the conventional methods. Our studies revealed the highest sensitivities of chromID MRSA and chromID CPS3 (> 90%). The sensivities for chromID ESBL and chromID S. aureus were > 70%. All media were also highly specific (97%). This specificity for chromID S.aureus and chromID CPS3 was 100%. We conclude, that all tested media showed good performance in presumptive identification and detection of resistance mechanisms.
Zaklad Mikrobiologii Farmaceutycznej Wydział Farmaceutyczny UJ CM, Kraków. budak@cm-uj.krakow.pl
Alicja
A
Budak
Marianna
M
Tokarczyk
Paweł
P
Nowak
Małgorzata
M
Skałkowska
Bozena
B
Bogusz
Małgorzata
M
Zwolińska-Wcisło
Marek
M
Wilk
D015373:Bacterial Typing Techniques / Q000379:methods*; D002863:Chromogenic Compounds; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D002863:Chromogenic Compounds; D003470:Culture Media