The infection of primary avian tracheal epithelial cells with infectious bronchitis virus.
Here we introduce a culture system for the isolation, passaging and amplification of avian tracheal epithelial (ATE) cells. The ATE medium, which contains chicken embryo extract and fetal bovine serum, supports the growth of ciliated cells, goblet cells and basal cells from chicken tracheas on fibronectin- or matrigel-coated dishes. Non-epithelial cells make up less than 10% of the total population. We further show that ATE cells support the replication and spread of infectious bronchitis virus (IBV). Interestingly, immunocytostaining revealed that basal cells are resistant to IBV infection. We also demonstrate that glycosaminoglycan had no effect on infection of the cells by IBV. Taken together, these findings suggest that primary ATE cells provide a novel cell culture system for the amplification of IBV and the in vitro characterization of viral cytopathogenesis.
Shen
Ching-I
CI
Department of Veterinary Medicine, National Chung-Hsing University, 250 Kuo-Kuang Rd., Taichung 402, Taiwan, Republic of China.
Wang
Ching-Ho
CH
Liao
Jiunn-Wang
JW
Hsu
Tien-Wang
TW
Kuo
Shu-Ming
SM
Su
Hong-Lin
HL
D000818:Animals; D004847:Epithelial Cells / Q000821:virology*; D006025:Glycosaminoglycans / Q000494:pharmacology; D001351:Infectious bronchitis virus / Q000187:drug effects / Q000502:physiology*; D020545:Respiratory Mucosa / Q000166:cytology*; D016662:Virus Integration / Q000187:drug effects / Q000502:physiology*; D014779:Virus Replication
D006025:Glycosaminoglycans