Prdm1a is necessary for posterior pharyngeal arch development in zebrafish.
Multiple tissue interactions and signaling within the pharyngeal arches are required for development of the craniofacial skeleton. Here, we focus on the role of the transcription factor prdm1a in the differentiation of the posterior skeleton. prdm1a is expressed in the presumptive pharyngeal arch region and later in an endodermal pouch, the otic vesicle, and pharyngeal teeth. prdm1a mutants display a reduction in pharyngeal arch markers, a loss of posterior ceratobranchial cartilages, and a reduction in most neural crest-derived dermal bones. This is likely caused by a decrease in the number of proliferating cells but not an increase in cell death. Finally, a reduction in two key developmental signaling pathways, Fgf and retinoic acid, alters prdm1a expression, suggesting that prdm1a expression is mediated by these signaling pathways to pattern the posterior craniofacial skeleton. Together, these results indicate an essential role for prdm1a in the development of the zebrafish craniofacial skeleton.
Birkholz
Denise A
DA
Department of Biomedical and Pharmaceutical Sciences, University of Montana, Center for Structural and Functional Neuroscience, Missoula, Montana, USA.
Olesnicky Killian
Eugenia C
EC
George
Kathleen M
KM
Artinger
Kristin Bruk
KB
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001934:Branchial Region / Q000033:anatomy & histology / Q000196:embryology*; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology / Q000378:metabolism; D005147:Facial Bones / Q000002:abnormalities / Q000033:anatomy & histology / Q000196:embryology; D005346:Fibroblast Growth Factors / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D009024:Morphogenesis / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D015398:Signal Transduction / Q000502:physiology; D012886:Skull / Q000002:abnormalities / Q000033:anatomy & histology / Q000196:embryology; D014212:Tretinoin / Q000378:metabolism; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D015415:Biomarkers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D029961:Zebrafish Proteins; D014212:Tretinoin; D005346:Fibroblast Growth Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1; C513257:prdm1a protein, zebrafish