Detection and genotyping of human papillomavirus DNA using polymerase chain reaction short PCR fragment 10-line probe assay in abnormal Papanicolaou-stained cervicovaginal smears.
OBJECTIVE
To evaluate the effectiveness of the short PCR fragment 10-line probe assay (SPF10-LiPA) system in detection of human papillomavirus (HPV) in abnormal Papanicolaou-stained cervicovaginal smears.


METHODS
We included 20 abnormal Papanicolaou-stained cervicovaginal smears. Samples were tested for HPV DNA detection and genotyping using the SPF10-LiPA system.


RESULTS
All samples amplified the INF150 gene control. In 2 of 20 cases, amplification of the viral sequences was negative. Of processed samples, 18 of 20 presented HPV infection; of them, 56% showed only 1 type of HPV infection; the remaining presented > or = 2 types of HPV (multiple infections). HPV16 was the most frequent specific viral in 60%, in single and multiple infections, followed by HPV18 (20%), HPV6 (10%) and HPV58 (10%). We also found HR-HPV45, 52, 58 and 68 and LR-HPV6, 11 and 70 viral DNA types. These multiple infections were detected with greater frequencies in atypical squamous cells of undetermined significance and in the low-grade squamous intraepithelial lesions.


CONCLUSIONS
The SPF10-LiPA system is efficient, sensitive, fast and simple for detecting and genotyping HPV DNA in abnormal Papanicolaou-stained cervicovaginal smears, which could be enormously useful in routine investigation for better clinical management of the patient.
de Méndez
Morelva Toro
MT
Department of Clinic Bioanalysis, University of Los Andes, Mérida, Venezuela. tmorelva@ula.ve
Bosch
Antonio Llombart
AL
D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology*; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D065006:Papanicolaou Test; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*
D004279:DNA, Viral