Hemoperfusion with polymyxin B-immobilized fiber column improves liver function after ischemia-reperfusion injury.
OBJECTIVE
To investigate the usefulness of direct hemoperfusion with a polymyxin B-immobilized fiber column (DHP-PMX therapy) for warm hepatic ischemia-reperfusion (I/R) injury after total hepatic vascular exclusion (THVE) using a porcine model.


METHODS
Eleven Mexican hairless pigs weighing 22-38 kg were subjected to THVE for 120 min and then observed for 360 min. The animals were divided into two groups randomly: the DHP-PMX group (n = 5) underwent DHP-PMX at a flow rate of 80 mL/min for 120 min (beginning 10 min before reperfusion), while the control group did not (n = 6). The rate pressure product (RPP): heart rate x end-systolic arterial blood pressure, hepatic tissue blood flow (HTBF), portal vein blood flow (PVBF), and serum aspartate aminotransferase (AST) levels were compared between the two groups.


RESULTS
RPP and HTBF were significantly (P < 0.05) higher in the DHP-PMX group than in the control group 240 and 360 min after reperfusion. PVBF in the DHP-PMX group was maintained at about 70% of the flow before ischemia and differed significantly (P < 0.05) compared to the control group 360 min after reperfusion. The serum AST increased gradually after reperfusion in both groups, but the AST was significantly (P < 0.05) lower in the DHP-PMX group 360 min after reperfusion.


CONCLUSIONS
DHP-PMX therapy reduced the hepatic warm I/R injury caused by THVE in a porcine model.
Sato
Hiroaki
H
Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
Oshima
Kiyohiro
K
Kobayashi
Katsumi
K
Yamazaki
Hodaka
H
Suto
Yujin
Y
Takeyoshi
Izumi
I
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001783:Blood Flow Velocity; D001794:Blood Pressure; D004195:Disease Models, Animal; D005260:Female; D006464:Hemoperfusion / Q000379:methods*; D008099:Liver / Q000098:blood supply / Q000503:physiopathology*; D008107:Liver Diseases / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D011112:Polymyxin B / Q000627:therapeutic use*; D011169:Portal Vein / Q000502:physiology; D015427:Reperfusion Injury / Q000503:physiopathology / Q000628:therapy*; D013552:Swine; D016896:Treatment Outcome
D001219:Aspartate Aminotransferases; D011112:Polymyxin B