Mast cell stabilization and antihistaminic potentials of Curculigo orchioides rhizomes.
OBJECTIVE
To investigate the mast cell stabilization and antihistaminic activities of the rhizomes of Curculigo orchioides (COR). Extract of Curculigo orchioides Gaertn. (Fam. Amaryllidaceae) has been reported to possess immunostimulant, and anti-inflammatory potentials. In Indian traditional system of medicine it is also used as anti-asthmatic and anti-inflammatory.


METHODS
Estimation of histamine release is key parameter for evaluating any target for its anti-allergic potential. The stabilization potential of the alcoholic extract of COR (100-400mg/kg) against mast cell degranulation was studied on isolated mice peritoneal mast cells. The antihistaminic activity was performed by determining the mortality rate of mice upon exposure to compound 48/80 and effect on inhibition of histamine release upon degranulation.


RESULTS
The raised number of intact mast cells intimates that the COR stabilized the mast cell degranulation (60.96+/-1.96%) and percentage antihistaminic potential of the extract (63.58+/-1.8 inhibition at dose of 400mg/kg) and it virtues further work towards the isolation of phytoconstituents from this plant.


CONCLUSIONS
This finding provides evidence that COR inhibits mast cell-derived immediate-type allergic reactions and mast cell degranulation.
Venkatesh
P
P
School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
Mukherjee
Pulok K
PK
Kumar
Satheesh N
SN
Nema
Neelesh K
NK
Bandyopadhyay
A
A
Fukui
Hiroyuki
H
Mizuguchi
Hiroyuki
H
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D031408:Curculigo / Q000737:chemistry*; D006633:Histamine Antagonists / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006633:Histamine Antagonists; D010936:Plant Extracts; D003189:p-Methoxy-N-methylphenethylamine