[Effect of glutamine on intestinal mucosal barrier function in rats with acute hepatic injury].
OBJECTIVE
To explore the role of glutamine in LPS and D-Gal induced acute hepatic injury.


METHODS
A total of 61 Wistar rats were randomly divided into three groups: control group, model group and GLN pretreated group. The animal model was established by LPS and D-Gal intraperitoneal injection. GLN at dose of 1 g/kg was intragastrically administrated for 7 d before intraperitoneal injection. To evaluate the hepatic injury, the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBiL) were detected by automatic biochemistry analysator. The liver and bowel tissue was observed by lightmicroscope and transmission electron microscope (TEM). The apoptosis of hepatocyte was detected by TUNEL. HPLC-PED was used in the study of intestinal permeability.


RESULTS
No significant differences were noted between ALT, AST, TBIL level, death rate and intestinal permeability (L/M) between model group and GLN pretreated group; In microscope, the confused structure of hepatic injury and inflammatory infiltration were similar between model group and GLN pretreated group. The injury of bowel was not obviously. Compared with the model group, there was better trend in liver and bowel in GLN pretreated group by transmission electron microscope (TEM). The apoptosis index in GLN pretreated group were lower than those in model group.


CONCLUSIONS
LPS can induce acute liver injury in D-Gal-sensitized rats.Glutamine has't the trend of protecting liver function and intestinal barrier function,decreasing death rates.
You an Hospital Attached to Capital University of Medical Science, Beijing 100069, China.
Hui
H
Wang
Chen-Zhao
CZ
Song
Qing-Hua
QH
Meng
Yi
Y
Ren
Fen-Yan
FY
Zhang
Jing-Jing
JJ
Zhao
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D005260:Female; D005973:Glutamine / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000293:injuries*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D005973:Glutamine