Small-molecule selectively recognizes human telomeric G-quadruplex DNA and regulates its conformational switch.
Structural complexity is an inherent feature of the human telomeric sequence, and it presents a major challenge for developing ligands of pharmaceutical interest. Recent studies have pointed out that the induction of a quadruplex or change of a quadruplex conformation on binding may be the most powerful method to exert the desired biological effect. In this study, we demonstrate a quadruplex ligand that binds selectively to different forms of the human telomeric G-quadruplex structure and regulates its conformational switch. The results show that not only can oxazine750 selectively induce parallel quadruplex formation from a random coil telomeric oligonucleotide in the absence of added cations, it also can easily surpass the energy barrier between two structures and change the G-quadruplex conformation in Na(+) or K(+) solution. The combination of its unique properties, including the size and shape of the G-quadruplex and the small molecule, is proposed as the predominant force for regulating the special structural formation and transitions. These results may stimulate the design of new quadruplex binders that would be capable of discriminating different G-quadruplex structures as well as controlling biological phenomena, functional molecules, and nanomaterials.
Chen
Mingli
M
State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, People's Republic of China.
Song
Guangtao
G
Wang
Chunyan
C
Hu
Dan
D
Ren
Jinsong
J
Qu
Xiaogang
X
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D054856:G-Quadruplexes / Q000187:drug effects*; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism* / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D012996:Solutions; D013379:Substrate Specificity; D016615:Telomere / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D010078:Oxazines; D012996:Solutions; C048170:oxazine 750; D004247:DNA; D012964:Sodium; D011188:Potassium