Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4-based vaccine vector development.
In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development.
Donofrio
Gaetano
G
Department of Animal Health, Sezione di Malattie Infettive degli Animali, Facoltà di Medicina Veterinaria, University of Parma, Parma, Italy. gaetano.donofrio@unipr.it
Franceschi
Valentina
V
Capocefalo
Antonio
A
Taddei
Simone
S
Sartori
Chiara
C
Bonomini
Sabrina
S
Cavirani
Sandro
S
Cabassi
Clotilde S
CS
Flammini
Cesidio F
CF
D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D015478:Antigens, Heterophile / Q000276:immunology / Q000378:metabolism*; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology; D026021:Herpesvirus 4, Bovine / Q000235:genetics / Q000276:immunology*; D021381:Protein Transport; D011817:Rabbits; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*; D014765:Viral Vaccines / Q000276:immunology*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015478:Antigens, Heterophile; D014764:Viral Proteins; D014765:Viral Vaccines