[Application of 293 cells in in vitro detection of carcinogens].
OBJECTIVE
To investigate the application of 293 cells to detect suspected carcinogens and provide experimental evidence by using in vitro cell transformation assay and tumorigenicity study.


METHODS
The transformation systems of cells cultured in vitro have been adopted to clarify the tumor promotive activity of Microcystin LR (MC-LR). The malignant transformation of 293 cells induced by MC-LR is tested by several methods including clone forming in soft agarose, serum requirement assay and tumor forming in mice to define the promotive activity of 293 cells.


RESULTS
293 cell acted like tumor cells after induced by MC-LR: serum dependence decreased, anchorage independence growth in soft agarose and formed cell clones, malignant tumors appeared in SCID mice.


CONCLUSIONS
293 cells were easy to culture and sensitive to environmental carcinogens so that can be used in detection of suspicious carcinogens.
The College of Life Science and Bio-engineering, Beijing University of Technology 100022, China.
Rui
R
Zhao
Lei
L
Zhang
Ru-Gang
RG
Zhong
Ling
L
Zhou
Yi
Y
Zeng
D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D006801:Humans; D008387:Marine Toxins; D051379:Mice; D016513:Mice, SCID; D052998:Microcystins / Q000633:toxicity*
D002273:Carcinogens; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR