Comparison of 2.5% sodium hypochlorite and 2% chlorhexidine gel on oral bacterial lipopolysaccharide reduction from primarily infected root canals.
BACKGROUND
This clinical study was conducted to compare the efficacy of chemomechanical preparation with 2.5% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) gel on eliminating oral bacterial lipopolysaccharide (LPS) in teeth with pulp necrosis and apical periodontitis.


METHODS
Fifty-four root canals were selected. Samples were collect before (s1) and after chemomechanical preparation (s2). Teeth were randomly divided into groups: GI, 2.5% NaOCl (n = 27) and GII, CHX gel (n = 27). Limulus amebocyte lysate assay was used to quantify endotoxins.


RESULTS
Endotoxin was present in 100% of the samples investigated, with a median value of 272 endotoxin units (EU)/mL (GI) and of 152.46 EU/mL (GII). As a result of chemomechanical preparation, LPS content was reduced to a median value of 86 EU/mL (GI) and 85 EU/mL (GII). Higher percentage value of endotoxin reduction was found in GI (P < .05).


CONCLUSIONS
The 2.5% NaOCl and 2% CHX gel were not effective in eliminating endotoxin from the primarily infected root canals.
Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil. bpgomes@fop.unicamp.br
Brenda P F A
BP
Gomes
Frederico C
FC
Martinho
Morgana E
ME
Vianna
D000293:Adolescent; D000328:Adult; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D002710:Chlorhexidine / Q000627:therapeutic use*; D002863:Chromogenic Compounds; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology* / Q000628:therapy; D004731:Endotoxins / Q000032:analysis; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis*; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology* / Q000628:therapy; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000627:therapeutic use*; D016896:Treatment Outcome; D055815:Young Adult
D000891:Anti-Infective Agents, Local; D002863:Chromogenic Compounds; D004731:Endotoxins; D008070:Lipopolysaccharides; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002710:Chlorhexidine