Oxidative stress and immune-suppression in Toxoplasma gondii positive blood donors: implications for safe blood transfusion.
The sero-markers of Toxoplasma gondii and oxidative stress (OS) were determined in a group of 260 blood donors attending blood banks in Greater Cairo. Twenty-four blood donors with the highest anti-T. gondii IgG titre were tested for IgG avidity. Of whom 4 (16.6%) had low IgG avidity antibodies, documenting recent infection, 6 (25%) had borderline avidity and 14 (58.3%) showed high avidity, ruling out recent infection. The plasma level of malondialdehyde (MDA) was significantly higher and activity of glutathione peroxidase (GSH-Px) and level of tocopherol (alpha, gamma, & lambda) fractions (P < 0.001) were lower in T. gondii-seropositive than in seronegative blood donors. This significant alteration in redox status between seropositive and seronegative donors suggested a degradation of their antioxidant enzymes caused by OS induced by increased free radicals attributable to toxoplasmosis infection. T. gondii infection also had a prominent influence on the association between OS biomarkers and immune-suppression status in seropositive donors.
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD, UK.
Hany M
HM
Elsheikha
Mona H
MH
El-Motayam
Mohamed Fathy
MF
Abouel-Nour
Ayman T A
AT
Morsy
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001782:Blood Donors; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D016036:Seroepidemiologic Studies; D024505:Tocopherols / Q000097:blood; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000097:blood* / Q000276:immunology
D000913:Antibodies, Protozoan; D015415:Biomarkers; D007074:Immunoglobulin G; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D024505:Tocopherols