The chicken or the egg: microRNA-mediated regulation of mRNA translation or mRNA stability.
In this issue of Molecular Cell, Fabian et al. (2009) demonstrate that in cell-free extracts from mouse Krebs-2 ascites, microRNA-mediated translational repression precedes target mRNA deadenylation, and identify GW182, PABP, and deadenylase subunits CAF1 and CCR4 as factors required for deadenylation.
Omer
Arina D
AD
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
Janas
Maja M
MM
Novina
Carl D
CD
D000818:Animals; D060565:Argonaute Proteins; D001201:Ascites / Q000235:genetics / Q000378:metabolism; D001324:Autoantigens / Q000378:metabolism; D001665:Binding Sites; D002287:Carcinoma, Krebs 2 / Q000235:genetics / Q000378:metabolism; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D039603:Eukaryotic Initiation Factor-4G / Q000378:metabolism; D005095:Exoribonucleases; D020868:Gene Silencing; D006801:Humans; D007700:Kinetics; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000235:genetics / Q000378:metabolism*; D054398:Receptors, CCR4 / Q000378:metabolism; D012097:Repressor Proteins; D012260:Ribonucleases
C526735:Ago2 protein, mouse; D060565:Argonaute Proteins; D001324:Autoantigens; C515355:Ccr4 protein, mouse; D015852:Eukaryotic Initiation Factor-2; D039603:Eukaryotic Initiation Factor-4G; D035683:MicroRNAs; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D012333:RNA, Messenger; D034743:RNA-Induced Silencing Complex; D054398:Receptors, CCR4; D012097:Repressor Proteins; C542687:mirnlet7 microRNA, mouse; C489778:Cnot7 protein, mouse; D005095:Exoribonucleases; D012260:Ribonucleases