[Mechanisms and effects of biosynthesis and apoptosis in repair of full-thickness skin defect with collagen-chitosan dermal stent].
OBJECTIVE
To investigate biosynthetic and apoptotic mechanisms in repair of full thickness skin defect with collagen-chitosan porous scaffold transplantation, and to determinate differences between wound repair with the scaffold transplantation and scar healing without the scaffold transplantation.


METHODS
The full thickness skin defects were made on 10 Bama miniature pigs and the bilayer dermal equivalent (BDE) composed of collagen-chitosan porous scaffold and silicone membrane was transplanted on wounds. Surfaces of wounds were observed at 1, 2, and 3 weeks after the BDE transplantation, and so were done the wound repairs after epidermis had been grafted for 2 weeks on surface of the scaffold which had been transplanted on skin defect wounds for 2 weeks. At the same time, TGF-beta1 expressions, apoptosis and self collagen replacement of scaffolds in wounds were detected in situ by immunohistochemical staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) and picrosirius red polarized light. Wounds without scaffold transplantation were studied as control.


RESULTS
1) Wounds with the scaffold transplantation were different from granulation tissue. 2) The peak of TGF-beta1 expression in the scaffold wounds was from 1 to 2 weeks after BDE transplantation, and TGF-beta1 expressions decreased continuously from 3 to 4 weeks. TGF-beta1 expressions increased continuously in the control wounds from 1 to 3 weeks and decreased on 4 weeks. TGF-beta1 expressions in the scaffold wounds on 1st and 2nd week were significantly higher than those in the corresponding control wounds, whereas, TGF-beta1 expressions in the scaffold wounds on 3rd and 4th week were significantly lower than those in the corresponding control wounds. 3) Apoptosis increased continuously in the scaffold wounds from 2 to 4 weeks after BDE transplantation, and so did in the control wounds from 3 to 4 weeks. However, apoptosis signals in the scaffold wounds on 2nd, 3rd, and 4th week after BDE transplantation were significantly more than those in the corresponding control wounds, and there was no difference between apoptosis signals in the scaffold wounds on 1st week after BDE transplantation and those in the corresponding control wounds. 4) Observation by picrosirius red polarized light method: self collagen began to synthesize in the scaffold wounds on 1st week after BDE transplantation, and scaffolds had been replaced by self collagen from 2 to 3 weeks after BDE transplantation.


CONCLUSIONS
Collagen-chitosan porous scaffold plays a very important role in wound healing of full thickness skin defect. The mechanisms of wound repair by dermal scaffold are different from those by granulation and scar healing. It has a good future in repairing skin defect.
Department of Plastic Surgery of the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China.
Shao-Jun
SJ
Xu
Ai-bin
AB
Huang
Lie
L
Ma
Jian-Ying
JY
Teng
Chang-You
CY
Gao
Zhi-Liang
ZL
Zhang
You-Di
YD
Ni
Sheng
S
Ye
Yong-Guang
YG
Wang
D000818:Animals; D017209:Apoptosis; D048271:Chitosan / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis* / Q000378:metabolism; D020405:Dermis; D005109:Extracellular Matrix; D005260:Female; D023422:Skin Irritancy Tests; D017768:Skin, Artificial; D015607:Stents; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing
D053773:Transforming Growth Factor beta1; D003094:Collagen; D048271:Chitosan