Vitrification of isolated human blastomeres.
This article describes a new methodology for preserving and banking isolated human blastomeres, whose originality is based on packing the blastomere into an emptied zona pellucida before vitrification. After warming, 75.7% of blastomeres survived and developed at a rate comparable to that in noncryopreserved blastomeres (62.5% cleavage, 26.6% compaction, and 20.3% cavitation).
Universitary Institute IVI Valencia, Instituto Valenciano de Infertilidad, Plaza Policía Local, 3, Valencia 46015, Spain. mjescriba@ivi.es
María-José
MJ
Escribá
Noelia
N
Grau
Laura
L
Escrich
Antonio
A
Pellicer
D001757:Blastomeres / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
D003451:Cryoprotective Agents