Impact of hydrodynamics on oral biofilm strength.
Mechanical removal of oral biofilms is ubiquitously accepted as the best way to prevent caries and periodontal diseases. Removal effectiveness strongly depends on biofilm strength. To investigate the influence of hydrodynamics on oral biofilm strength, we grew single- and multi-species biofilms of Streptococcus oralis J22, Actinomyces naeslundii TV14-J1, and full dental plaque at shear rates ranging from 0.1 to 50 1/sec and measured their compressive strength. Subsequently, biofilm architecture was evaluated by confocal laser scanning microscopy. Multi-species biofilms were stronger than single-species biofilms, with strength values ranging from 6 to 51 Pa and from 5 to 17 Pa, respectively. In response to increased hydrodynamic shear, biofilm strength decreased, and architecture changed from uniform carpet-like to more "fluffy" with higher thickness. S. oralis biofilms grown under variable shear of 7 and 50 1/sec possessed properties intermediate of those measured at the respective single shears.
Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
E
E
Paramonova
O J
OJ
Kalmykowa
H C
HC
van der Mei
H J
HJ
Busscher
P K
PK
Sharma
D000190:Actinomyces / Q000166:cytology / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms; D001696:Biomechanical Phenomena; D019245:Compressive Strength; D003773:Dental Plaque / Q000382:microbiology*; D055119:Elastic Modulus; D006801:Humans; D018613:Microscopy, Confocal; D033081:Shear Strength; D019533:Streptococcus oralis / Q000166:cytology / Q000502:physiology*; D013314:Stress, Mechanical