Halofuginone has a beneficial effect on gentamicin-induced acute nephrotoxicity in rats.
The aim of this study was to investigate the putative beneficial effect of halofuginone on gentamicin-induced nephrotoxicity in rats. Sprague-Dawley rats were treated with gentamicin sulphate (GEN; 80 mg/kg) or saline intraperitoneally (i.p.) for 7 days. Halofuginone was administered (0.1 mg/kg/day; i.p.) following GEN or saline injections. Blood and urine samples were collected to measure the renal function tests. Kidneys were excised for histological evaluation and for the measurement of malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO) activity, and chemiluminescence (CL). Halofuginone treatment to animals with GEN-induced renal injury caused a significant decrease in serum blood urea nitrogen level and reduced the elevated MDA, GSH content, and MPO activity. It was also effective in reversing the elevated CL values of rats with GEN-induced nephrotoxicity and preserving renal morphology, as examined microscopically. In conclusion, halofuginone was beneficial in GEN-induced acute nephrotoxicity. The mechanism could be attributed, at least in part, to decreased tissue leukocyte infiltration and reactive metabolite production.
Karakoyun
Berna
B
Department of Nursing, Marmara University, Health Sciences Faculty, Istanbul, Turkey.
Yuksel
Meral
M
Turan
Pinar
P
Arbak
Serap
S
Alican
Inci
I
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D052999:Quinazolinones / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051437:Renal Insufficiency / Q000139:chemically induced*
D000975:Antioxidants; D002712:Chlorides; D005839:Gentamicins; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D010880:Piperidines; D010936:Plant Extracts; D052999:Quinazolinones; D008315:Malondialdehyde; C010176:halofuginone