The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths.
DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25ng) compared to the absence of RNA (5ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications.
Department of Chemistry, University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom.
Kirsty J
KJ
Shaw
Lauren
L
Thain
Peter T
PT
Docker
Charlotte E
CE
Dyer
John
J
Greenman
Gillian M
GM
Greenway
Stephen J
SJ
Haswell
D004247:DNA / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012313:RNA / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction
D012313:RNA; D012822:Silicon Dioxide; D004247:DNA