[Effects of mutational sptl5 gene to xylose utilization of Saccharomyces cerevisiae].
We used genetic methods to get a mutational spt15 gene from the recombinant strain Saccharomyces cerevisiae YPH499-3, screened by global transcription machinery engineering (gTME) approach. We transformed the gene into the original strain Saccharomyces cerevisiae YPH499 using the vector pYX212, then got a new recombinant strain. We studied the characteristic of this strain and found that it could metabolize xylose and co-ferment xylose and glucose. Under the fermentation condition of 30 degrees C, 200 r/min, 72 h, the utilization ratio of xylose was 82.0%, with 32.4% of ethanol yield when the carbon source in the media was 50 g/L xylose, while the utilization ratio of xylose and glucose was 80.4% and 100% respectively, with the 31.4% of ethanol yield when the carbon source was 50 g/L glucose/xylose (1:1). Meanwhile, the concentration of the by-product xylitol was very low. This study demonstrates the effect which the forward mutation of spt15 gene makes to the co-fermentation of xylose and glucose to ethanol by Saccharomyces cerevisiae.
State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
Hongmei
H
Liu
Wen
W
Tang
Cangang
C
Lai
Ming
M
Yan
Lin
L
Xu
Pingkai
P
Ouyang
D001483:Base Sequence; D000431:Ethanol / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D035181:TATA-Box Binding Protein / Q000235:genetics*; D014170:Transformation, Genetic; D014994:Xylose / Q000378:metabolism*
C115017:SPT15 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D000431:Ethanol; D014994:Xylose; D005947:Glucose