Study of antimutagenic and anticytotoxic effects of biorag in case of mutations induced by ammonium nitrate.
The antimutagenic and anticytotoxic effects of bioactivator - biorag (created by Prof. R. Gakhokidze) in the case of mutations induced by ammonium nitrate (NH(4)NO(3)) were studied on the laboratory mice. The cytogenetic and toxicological methods of investigation were used in our research. Ammonium nitrate is characterized by mutagenic, cytotoxic and consequently general toxic action. Introduction of ammonium nitrate (doze 1/2, 1/5 LD(50)) per oral to animals induces strong increase (p<0,001) of frequency of chromosomal aberrations (multiple fragmentation, lyses), a genomic mutations (triploidy, tetraploidy), pathological mitosis (K-mitosis, hollow metaphase, adhesion of chromosomes) and destruction of interphase nucleuses (hollow nucleus). Biorag is characterized with greatly expressed antimutagenic and anticytotoxic effect and statistically reliable reduces mutagenic and cytotoxic effect of ammonium nitrate. At separate effect of ammonium nitrate (dose 1/2 LD(50)) the frequency of chromosomal anomalies was 8,8%, pathologic mitosis - 21,4%, interphase nucleus destruction - 4,5%. After addition of biorag in diet, these indexes decreases accordingly to 3,0%; 8,6% and 1,5%. (p<0,001). On the base of conducted experiments application of biorag for medical purpose is prospective, especially for people who are in contact with harmful, mutagenic substances, also for the individuals poisoned with pesticides and fertilizers.
I. Javakhishvili Tbilisi State University.
A
A
Pirtskhelani
R
R
Gakhokidze
N
N
Pirtskhelani
E
E
Gakhokidze
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects*; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis / Q000187:drug effects; D009154:Mutation / Q000187:drug effects; D009566:Nitrates / Q000037:antagonists & inhibitors* / Q000633:toxicity
D016587:Antimutagenic Agents; D009566:Nitrates; C006568:ammonium nitrate