Determination of paromomycin residues in turkey tissues by liquid chromatography/mass spectrometry.
A liquid chromatography-electrospray-mass spectrometry method has been developed and validated for the determination of paromomycin in turkey muscle, liver and kidney, using kanamycin as internal standard. The method consists of solid-phase extractions on mixed-mode columns. The chromatographic separation was carried out on a C(18) column using binary gradient elution containing acetonitrile and 5mM pentafluoropropionic-acid in water. The method was evaluated for specificity, linearity, recovery, accuracy, limit of detection, limit of quantification, intra- and inter-day repeatability, and stability. It was proven to be selective, linear, precise and accurate over the concentration range tested (0.5xMRL-2xMRL for each tissue) with correlation coefficients >0.990. The method was successfully used for the residue determination of PARO from edible tissues of turkeys.
Róna
K
K
Semmelweis University, Department of Forensic and Insurance Medicine, Ulloi út 93, 1091 Budapest, Hungary.
Klausz
G
G
Keller
E
E
Szakay
M
M
Laczay
P
P
Shem-Tov
M
M
Székely-Körmöczy
P
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D009132:Muscles / Q000737:chemistry*; D010303:Paromomycin / Q000032:analysis*; D012680:Sensitivity and Specificity; D014422:Turkeys
D000900:Anti-Bacterial Agents; D010303:Paromomycin