Molecular analysis of an actin gene, CarACT1, from chickpea (Cicer arietinum L.).
Actins are ubiquitous and highly conserved proteins that play key roles in cell formation and cellular activities. In this study, an actin gene was isolated from chickpea for the first time and designated as CarACT1 (for Cicer arietinum L. actin gene 1; Genbank accession no. EU529707). It encoded a putative protein with 377 amino acids and contained five exons and four introns within genomic DNA sequence. CarACT1 was localized in cytoplasm and showed high similarity to other well known actins from various species. Reverse transcription-polymerase chain reaction (RT-PCR) assay proved that CarACT1 transcripts were ubiquitously accumulated in all major organs, such as seedling roots, stems, leaves, flowers, young pods, and seeds, as well as in diverse developmental stages, such as leaf senescence, seed development and germination. Our results suggested that CarACT1 is an actin gene with physiological functions and may be served as a potential reference gene for transcription level of interesting genes in chickpea.
State Key Laboratory of Crop Genetics and Germplasm, Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, 210095 Nanjing, China.
Hui
H
Peng
Huiying
H
Cheng
Xingwang
X
Yu
Qinghua
Q
Shi
Hua
H
Zhang
Jiangui
J
Li
Hao
H
Ma
D000199:Actins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D029863:Cicer / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination / Q000235:genetics; D008958:Models, Molecular; D010802:Phylogeny; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
D000199:Actins