Validation and application of a method for the determination of total chromium in rat tissues by inductively coupled plasma mass spectrometry.
The validation of a method for the determination of chromium (Cr) in F-344/N rat tissues by inductively coupled plasma-mass spectrometry is described. Samples were analyzed after a rapid, open-vessel microwave digestion procedure. Performance of the method was evaluated using kidney tissue across a concentration range of 0.50-5.00 microg Cr/g tissue. Data for method linearity, accuracy, precision, digest stability, and storage stability are presented along with limits of detection and quantitation data. Data from a method cross-validation for B6C3F1 mouse kidney tissue are also presented. After validation, the method was applied to analyze samples collected in support of two chronic toxicity and carcinogenesis studies conducted by the National Toxicology Program.
Levine
Keith E
KE
RTI International, Research Triangle Park, NC 27709, USA. levine@rti.org
Stout
Matthew D
MD
Ross
Glenn T
GT
Essader
Amal S
AS
Weber
Frank X
FX
Grohse
Peter M
PM
Fernando
Reshan A
RA
Milstein
Lisa S
LS
Hooth
Michelle J
MJ
Collins
Bradley J
BJ
D000818:Animals; D002857:Chromium / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004355:Drug Stability; D007668:Kidney / Q000737:chemistry*; D057230:Limit of Detection; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D051381:Rats; D011916:Rats, Inbred F344; D012680:Sensitivity and Specificity
D002857:Chromium