Phased control of expansin activity during leaf development identifies a sensitivity window for expansin-mediated induction of leaf growth.
Expansins are cell wall proteins associated with the process of plant growth. However, investigations in which expansin gene expression has been manipulated throughout the plant have often led to inconclusive results. In this article, we report on a series of experiments in which overexpression of expansin was targeted to specific phases of leaf growth using an inducible promoter system. The data indicate that there is a restricted window of sensitivity when increased expansin gene expression leads to increased endogenous expansin activity and an increase in leaf growth. This phase of maximum expansin efficacy corresponds to the mid phase of leaf growth. We propose that the effectiveness of expansin action depends on the presence of other modulating factors in the leaf and we suggest that it is the control of expression of these factors (in conjunction with expansin gene expression) that defines the extent of leaf growth. These data help to explain some of the previously observed variation in growth response following manipulation of expansin gene expression and highlight a potential linkage of the expression of modifiers of expansin activity with the process of exit from cell division.
Sloan
Jennifer
J
Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Backhaus
Andreas
A
Malinowski
Robert
R
McQueen-Mason
Simon
S
Fleming
Andrew J
AJ
D002455:Cell Division; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
D010940:Plant Proteins; C095825:expansin protein, plant; D005966:Glucuronidase