Pax-3 and Pax-7 label muscle progenitor cells during myotomal myogenesis in Coregonus lavaretus (Teleostei: Coregonidae).
In Coregonus lavaretus, prior the mesoderm segmentation, in cells adjacent to the notochord called adaxial cells MyoD and slow myosin heavy chain (MyHC-slow) proteins were observed. After somite formation, adaxial cells migrate towards the lateral part of the myotome and form a layer of red muscles. Deeper cells differentiate into white muscle fibres. In situ hybridization using Pax-3 molecular probe revealed, that after somitogenesis, Pax-3 is expressed in a layer of cells superficial to the myotome resembling the "external cells" (found in many teleosts species) or dermomyotome described in Amniota. During later developmental stages Pax-3 gene is expressed in cells in intermyotomal space and then in myoblasts between myotubes. In these cells Pax-7 protein was also observed. Pax-3/7 positive cells which have migrated into the myotomes differentiate into satellite cells/secondary myoblasts and participate in hypertrophic and hyperplastic growth of muscles.
Department of Animal Developmental Biology, Zoological Institute, University of Wroclaw, Sienkiewicza 21, Wroclaw 50-335, Poland.
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Kacperczyk
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Jagla
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Daczewska
D000818:Animals; D008648:Mesoderm / Q000196:embryology; D024510:Muscle Development / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018485:Muscle Fibers, Skeletal; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development; D017570:MyoD Protein / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D009672:Notochord / Q000196:embryology; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor / Q000235:genetics* / Q000378:metabolism*; D051761:Paired Box Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D012487:Salmonidae / Q000196:embryology / Q000378:metabolism*; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology; D019170:Somites / Q000196:embryology; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*
D017570:MyoD Protein; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor; D051761:Paired Box Transcription Factors; D029961:Zebrafish Proteins; C489412:pax3a protein, zebrafish; D018995:Myosin Heavy Chains