DNA damage and repair of head and neck cancer cells after radio- and chemotherapy.
DNA repair is critical for successful chemo- and radiotherapy of human tumours, because their genotoxic sensitivity may vary in different types of cancer cells. In this study we have compared DNA damage and the efficiency of its repair after genotoxic treatment with hydrogen peroxide, cisplatin and gamma-radiation of head and neck squamous cell carcinoma (HNSCC). Lymphocytes and tissue cells from biopsies of 37 cancer patients and 35 healthy donors as well as the HTB-43 larynx cancer cell line were employed. The cell sensitivity to genotoxic treatment was estimated by the MTT survival assay. The extent of DNA damage and efficiency of its repair was examined by the alkaline comet assay. Among the examined treatments, we found that HNSCC cells were the most sensitive to gamma-radiation and displayed impaired DNA repair. In particular, DNA damage was repaired less effectively in cells from HNSCC metastasis than healthy controls. In conclusion, our results suggest that the different genotoxic sensitivity of HNSCC cells may depend on their DNA repair capacity what in turn may be connected with the effectiveness of head and neck cancer therapy.
Department of Molecular Genetics, University of Lodz, Banacha 12/16 Street, 90-237 Lodz, Poland.
Pawel
P
Rusin
Anna
A
Walczak
Anita
A
Zwierzchlejska
Jurek
J
Olszewski
Alina
A
Morawiec-Bajda
Dariusz
D
Kaczmarczyk
Krzysztof
K
Kusmierczyk
Pawel
P
Garncarek
Dariusz
D
Pytel
Tomasz
T
Sliwinński
Ireneusz
I
Majsterek
D000328:Adult; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000532:radiotherapy; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002945:Cisplatin / Q000627:therapeutic use; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000532:radiotherapy; D006801:Humans; D006861:Hydrogen Peroxide / Q000627:therapeutic use; D007700:Kinetics; D008297:Male; D009362:Neoplasm Metastasis; D011878:Radiotherapy
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D006861:Hydrogen Peroxide; D002945:Cisplatin