Enhancement of guided-wave two-photon-excited luminescence response with a photonic-crystal fiber.
Photonic-crystal fiber (PCF) is shown to substantially increase the guided-wave luminescent response from molecules excited through two-photon absorption (TPA) by femtosecond near-infrared laser pulses. With only a few nanoliters of TPA-excited molecules filling airholes in a specifically designed PCF, the guided-wave two-photon-excited luminescence (TPL) signal is enhanced by more than 2 orders of magnitude relative to the maximum TPL signal attainable from a cell with the same dye excited and collected by the same PCF. Biophotonic implications of this waveguide TPL-response enhancement include fiber-format solutions for online monitoring of drug delivery and drug activation, interrogation of neural activity, biosensing, endoscopy, and locally controlled singlet oxygen generation in photodynamic therapy.
Fedotov
Ilya V
IV
Physics Department, International Laser Center, M. V. Lomonosov Moscow State University, Vorob'evy gory, Moscow 119992, Russia.
Fedotov
Andrei B
AB
Doronina
Lyubov V
LV
Zheltikov
Aleksei M
AM
D017076:Computer-Aided Design; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008029:Lighting / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D055100:Optical Fibers; D017785:Photons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity