Comparison of the effects of low intra-abdominal pressure and pentoxifylline on oxidative stress during CO2 pneumoperitoneum in rabbits.
BACKGROUND
The aim of this study was to investigate the effect of low-pressure pneumoperitoneum and pentoxifylline, a methylxanthine derivative, in the prevention of injury caused by free oxygen radicals generated during CO(2 )pneumoperitoneum.


METHODS
Twenty-eight rabbits were allocated randomly to 4 groups. Control group rabbits (group 1) were subjected to anesthesia for 60 min; group 2 and 3 animals were subjected to a CO(2) pneumoperitoneum (15 or 7 mm Hg); and group 4 rabbits received 50 mg/kg pentoxifylline, followed by a 15-mm-Hg pneumoperitoneum. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), lipid hydroperoxide, glutathione reductase and total antioxidant status were measured.


RESULTS
Compared with group 1, a significant increase in lipid hydroperoxide levels at the end of the pneumoperitoneum and 30 min after deflation and a significant decrease in total antioxidant status 24 h after deflation were recorded in group 2. In addition, a significant increase was observed in ALT, AST and LDH levels. These changes were attenuated by low-pressure pneumoperitoneum, whereas pentoxifylline pretreatment appeared to attenuate only transaminase levels.


CONCLUSIONS
Low-pressure pneumoperitoneum could attenuate ischemia/reperfusion injury induced by CO(2 )pneumoperitoneum in a rabbit model whereas pentoxifylline pretreatment appeared to attenuate only transaminase levels. Pentoxifylline did not prevent the development of oxidative stress.
Nesek-Adam
V
V
Department of Anesthesiology, Resuscitation and Intensive Care, University of Zagreb, Zagreb, Croatia. tihomir.adam@zg.htnet.hr
Vnuk
D
D
Rasić
Z
Z
Rumenjak
V
V
Kos
J
J
Krstonijević
Z
Z
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002245:Carbon Dioxide; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010431:Pentoxifylline / Q000494:pharmacology*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011312:Pressure; D011817:Rabbits; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control
D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D002245:Carbon Dioxide; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D010431:Pentoxifylline