[Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production].
Candida glycerinogenes WL2002-5 (C.g) is an important industrial strain for glycerol production. To further improve glycerol production, we reconstructed a binary vector pCAM3300-zeocin-CgGPD1, introduced it to Agrobacterium tumefaciens LBA4404 by electroporation, and then transformed the T-DNA harboring the CgGPD1 to Candida glycerinogenes by Agrobacterium tumefaciens-mediated transformation (ATMT). After 96 h fermentation with glucose as the substrate, we screened a transformant named C.g-G8 with high glycerol production. Compared with the wild strain, the glucose consumption rate of C.g-G8 and the glycerol production were 12.97% and 18.06% higher, respectively. During the fermentation, the activity of glycerol-3-phosphate dehydrogenase of C.g-G8 was 27.55% higher than that of the wild strain. The recombinant Candida glycerinogenes with high glycerol production was successful constructed by ATMT method.
Liu
Ailing
A
The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Rao
Zhiming
Z
Ma
Zheng
Z
Zhuge
Bin
B
Fang
Huiying
H
Zhuge
Jian
J
D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics*; D002175:Candida / Q000235:genetics* / Q000378:metabolism; D018274:Electroporation; D005285:Fermentation; D005990:Glycerol / Q000032:analysis* / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D005993:Glycerolphosphate Dehydrogenase; D005990:Glycerol