[The analysis of "alpha" dominant mutation of hepatitis B virus in community-based Zhengding area, Hebei province].
OBJECTIVE
To determine the "alpha"dominant mutation of hepatitis B virus (HBV) in community-based Zhengding. Analysis the role of the newborn hepatitis B vaccination on the mutation.


METHODS
Based on the national surveillance of hepatitis B, 11,478 people's sera were collected and tested by SPRIA with kits. Collect people's sera with positive HBsAg and amplify the S gene. Sequencing and clastwaling them with the standard sequences.


RESULTS
Overall, HBV DNA was successfully amplified and sequenced in 434 of 443 samples. 6.7% samples mutated in HBV "alpha" dominant region. The difference between the mutation ratio of the two loops of HBV "alpha" dominant between the people born before and after the year 1986 has no significance.


CONCLUSIONS
There were HBV "alpha" dominant mutant virus in the local area with a low infection rate in the population born after the year 1986. It could not explain the newborn hepatitis B vaccination can induce the prevalence of the "alpha" dominant mutate HBV.
Yan
Hong-Xia
HX
Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Ma
Jing-Chen
JC
Bian
Tao
T
Huang
Chang-Hong
CH
Zhang
Shuang
S
Zhang
Zhi-Yong
ZY
Hao
Zhi-Yong
ZY
Chen
Ji-Chao
JC
Zhang
Yong
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D006509:Hepatitis B / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D055815:Young Adult
D006514:Hepatitis B Surface Antigens