Single DNA/protein studies with magnetic traps.
Magnetic traps provide a simple technique to pull and twist a variety of biomolecules and monitor the resulting change in extension. They have been used with great success to investigate the interaction of stretched and supercoiled DNA and DNA fibers (e.g. chromatin) with a great variety of enzymes. In this small review we will address their recent use in the study of topoisomerases, gyrase, DNA translocases and various structural proteins.
Meglio
Adrien
A
ENS, LPS, UMR 8550 CNRS, 24 rue Lhomond, 75005 Paris, France; Universite Paris 6, Universite Paris 7, 4 place Jussieu, 75005 Paris, France.
Praly
Elise
E
Ding
Fangyuan
F
Allemand
Jean-Francois
JF
Bensimon
David
D
Croquette
Vincent
V
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D006801:Humans; D008280:Magnetics; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
D004277:DNA, Single-Stranded; D004798:Enzymes; D011506:Proteins