High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample).
Mellmann
A
A
Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch-Str. 41, D-48149 Münster, Germany. mellmann@uni-muenster.de
Bimet
F
F
Bizet
C
C
Borovskaya
A D
AD
Drake
R R
RR
Eigner
U
U
Fahr
A M
AM
He
Y
Y
Ilina
E N
EN
Kostrzewa
M
M
Maier
T
T
Mancinelli
L
L
Moussaoui
W
W
Prévost
G
G
Putignani
L
L
Seachord
C L
CL
Tang
Y W
YW
Harmsen
D
D
D001420:Bacteria, Aerobic / Q000737:chemistry* / Q000145:classification*; D001424:Bacterial Infections / Q000175:diagnosis*; D003951:Diagnostic Errors / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*