Lack of correlation between p53 codon 72 polymorphism and anal cancer risk.
OBJECTIVE
To investigate the potential role of p53 codon 72 polymorphism as a risk factor for development of anal cancer.


METHODS
Thirty-two patients with invasive anal carcinoma and 103 healthy blood donors were included in the study. p53 codon 72 polymorphism was analyzed in blood samples through polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing.


RESULTS
The relative frequency of each allele was 0.60 for Arg and 0.40 for Pro in patients with anal cancer, and 0.61 for Arg and 0.39 for Pro in normal controls. No significant differences in distribution of the codon 72 genotypes between patients and controls were found.


CONCLUSIONS
These results do not support a role for the p53 codon 72 polymorphism in anal carcinogenesis.
Department of Surgery, Division of Coloproctology, Clinicas Hospital of Porto Alegre and Federal University of Rio Grande do Sul, 90.035-930 Porto Alegre, Brazil.
Simone S
SS
Contu
Grasiela
G
Agnes
Andrea P
AP
Damin
Paulo C
PC
Contu
Mário A
MA
Rosito
Claudio O
CO
Alexandre
Daniel C
DC
Damin
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001005:Anus Neoplasms / Q000453:epidemiology / Q000235:genetics*; D003062:Codon; D005260:Female; D005787:Gene Frequency; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D013223:Statistics as Topic
D003062:Codon