[Molecular imprinting-flow injection chemiluminescence method for determination of doxycycline hydrochloride].
Doxycycline hydrochloride can enhance the chemiluminescence of potassium ferricyanide and luminol in alkaline medium. So a molecular imprinting-flow-injection chemiluminescence method for the determination of doxycycline hydrochloride was established by using doxycycline hydrochloride-imprinted polymers as recognition material and potassium ferricyanide and luminol as detection system. Doxycycline hydrochloride-imprinted polymer was synthesized using methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker. The linear range is 9.0 x 10(-7)-6.0 x 10(-5) g x mL(-1) and the detection limit is 3.2 x 10(-7) g x mL(-1). The relative standard deviation for 6.0 X 10(-6) g x mL(-1) of doxycycline hydrochloride was 3.5% (n = 9). This method has been successfully applied to the determination of doxycycline hydrochloride in tablets and in urine samples.
Zhou
Yan-Mei
YM
College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China. zhouym@henu.edu.cn
Zhang
Cheng-Li
CL
Lei
Jian-Du
JD
Ma
Tong-Sen
TS
Ma
Guang-Hui
GH
Su
Zhi-Guo
ZG
Chao
Yang
Y
D000327:Adsorption; D002138:Calibration; D004318:Doxycycline / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D005292:Ferricyanides / Q000737:chemistry; D017022:Flow Injection Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis*; D013997:Time Factors
D005292:Ferricyanides; D008689:Methacrylates; D011108:Polymers; D008165:Luminol; C004919:ethylene dimethacrylate; D004318:Doxycycline; C028033:potassium ferricyanide