Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria.
In this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.
Miller
Christopher H
CH
School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.
Nisa
Shahista
S
Dempsey
Sandi
S
Jack
Cameron
C
O'Toole
Ronan
R
D000995:Antitubercular Agents / Q000494:pharmacology*; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development*; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000254:growth & development; D016566:Organoselenium Compounds / Q000494:pharmacology; D017279:Selenocysteine / Q000031:analogs & derivatives; D054852:Small Molecule Libraries
D000995:Antitubercular Agents; D016566:Organoselenium Compounds; D054852:Small Molecule Libraries; D017279:Selenocysteine; D003545:Cysteine; C002979:selenomethylselenocysteine