The LIM domain protein Wtip interacts with the receptor tyrosine kinase Ror2 and inhibits canonical Wnt signalling.
Wtip is a LIM domain protein of the Ajuba/Zyxin family involved in kidney and neural crest development; Ror2 is a receptor tyrosine kinase involved in the development of skeleton, heart, lung, genitalia and kidneys. Here we describe Wtip as an intracellular interaction partner of Ror2. Full-length Ror2 recruits Wtip to the cell membrane, a mutant involved in human disease fails to do so. Both genes and proteins show overlapping expression in the mouse embryo. We show that Wtip is able to inhibit canonical Wnt signalling in mammalian cells and in Xenopus embryos linking Wtip to a crucial developmental pathway.
Max Planck-Institute for Molecular Genetics, Development and Disease Group, 14195 Berlin, Germany.
Nicole Verhey
NV
van Wijk
Florian
F
Witte
Ann Carolin
AC
Feike
Alexandra
A
Schambony
Walter
W
Birchmeier
Stefan
S
Mundlos
Sigmar
S
Stricker
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D056970:Co-Repressor Proteins; D003598:Cytoskeletal Proteins; D006801:Humans; D051379:Mice; D017434:Protein Structure, Tertiary; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D051153:Wnt Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014981:Xenopus
D002352:Carrier Proteins; D056970:Co-Repressor Proteins; D003598:Cytoskeletal Proteins; D051153:Wnt Proteins; C484858:Wtip protein, mouse; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; C477010:Ror2 protein, mouse