A highly selective fluorescent ESIPT probe for the dual specificity phosphatase MKP-6.
A highly selective fluorescent probe for a protein tyrosine phosphatase (PTP) was designed by a simple phosphorylation of the 2-(2'-hydroxyphenyl)benzothiazole (HBT) chromophore: upon selective enzymatic hydrolysis, an excited-state intramolecular proton transfer (ESIPT) occurs, resulting in a large Stokes shift.
Kim
Tae-Il
TI
Department of Chemistry, Dankook University, 126 Jukjeon-dong, Yongin-si, Gyeonggi-do, 448-701, Korea.
Kang
Hyo Jin
HJ
Han
Garam
G
Chung
Sang J
SJ
Kim
Youngmi
Y
D054637:Dual-Specificity Phosphatases / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D054639:Mitogen-Activated Protein Kinase Phosphatases / Q000378:metabolism*; D010766:Phosphorylation
D005456:Fluorescent Dyes; D054639:Mitogen-Activated Protein Kinase Phosphatases; C498843:DUSP14 protein, human; D054637:Dual-Specificity Phosphatases