Altered protein-expressing profile in hPNAS4-induced apoptosis in A549 human lung adenocarcinoma cells.
Human PNAS4 (hPNAS4) is a recently identified pro-apoptosis gene, which is able to induce apoptosis in A549 human lung adenocarcinoma cells following its overexpression. In this work, we investigated the changes of protein profile in hPNAS4-induced apoptosis in A549 cells through proteomic strategy consisting of two-dimensional electrophoresis (2-DE) coupled with MALDI-Q-TOF mass spectrometry. A total of 20 different proteins with more than 3.0-fold change in expression, including 5 up-regulated and 15 down-regulated proteins were successfully identified by database search. The mRNA transcription levels of the different proteins were further examined by RT-PCT. Functional analyses showed these different proteins are involved in diverse biological processes including metabolism, proteolysis, signal transduction, apoptosis, and redox regulation. Two essential apoptosis-associated protein, annexin A1 and prothymosin alpha, were confirmed by Western blot and showed consistent changes with proteomic detection. Our data provide molecular evidence and possible associated pathway in hPNAS4-induced apoptosis through proteomic strategy, which should be contributed to further investigation on biological function of hPNAS4.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, Sichuan Province, China.
Lan-Tu
LT
Gou
Ai-Ping
AP
Tong
Fei
F
Yan
Zhu
Z
Yuan
Fei
F
He
Wei
W
Wang
Yan
Y
Zhou
Li-Juan
LJ
Chen
Ming-Hai
MH
Tang
Jin-Liang
JL
Yang
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017305:Annexin A1 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D019759:Carbon-Nitrogen Lyases; D045744:Cell Line, Tumor; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010084:Oxidation-Reduction; D011498:Protein Precursors / Q000378:metabolism; D015398:Signal Transduction; D013947:Thymosin / Q000031:analogs & derivatives / Q000378:metabolism; D015854:Up-Regulation
D017305:Annexin A1; D051017:Apoptosis Regulatory Proteins; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin; D019759:Carbon-Nitrogen Lyases; C000604991:DESI2 protein, human