Differential immunoproteomics enables identification of Metarhizium anisopliae proteins related to Rhipicephalus microplus infection.
Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differentially secreted proteins led to the identification of proteases (carboxypeptidase and Pr1A), chitinase, carboxylic acid transport and proline-rich protein. Differential immunoproteomics strategy facilitated the detection and the identification of new proteins related to M. anisopliae host-pathogen interaction and could be used in further works to identify novel proteins related to other microbial infection systems.
Santi
Lucélia
L
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil.
Silva
Walter Orlando Beys da
WO
Pinto
Antônio Frederico Michel
AF
Schrank
Augusto
A
Vainstein
Marilene Henning
MH
D000818:Animals; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics; D015151:Immunoblotting; D052981:Metarhizium / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D048494:Rhipicephalus / Q000378:metabolism / Q000382:microbiology / Q000472:pathogenicity*; D013984:Tick Infestations / Q000382:microbiology
D005656:Fungal Proteins; D020543:Proteome