Visualizing the beating heart in Drosophila.
The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in myogenic hearts. A key step in examining heart function in the fly is finding a way to access the heart in a manner that preserves its myogenic function while still allowing the beating heart organ to be observed and recorded. Two different methods for observing and recording the beating heart in both larva and adult Drosophila are described here. Our semi-intact preparation using adult flies allows clear visualization of the abdominal heart without interference from the pigmented cuticle and overlying fat bodies. To record larval heart beats it is necessary to immobilize the larva, which minimizes body wall movements thereby reducing heart movements that are not associated with myocardial contractions. Our methodologies produce stable adult and larval heart preparations that can beat for hours at rates of 1-3 Hz.
Vogler
Georg
G
Development and Aging Program, The Burnham Institute for Medical Research.
Ocorr
Karen
K
D000818:Animals; D004330:Drosophila / Q000502:physiology*; D006321:Heart / Q000502:physiology*; D009200:Myocardial Contraction / Q000502:physiology; D055095:Optics and Photonics / Q000379:methods