Purification of Arabidopsis argonaute complexes and associated small RNAs.
Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.
Qi
Yijun
Y
National Institute of Biological Sciences, Beijing, China.
Mi
Shijun
S
D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D060565:Argonaute Proteins; D015153:Blotting, Western; D019175:DNA Methylation; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000737:chemistry / Q000302:isolation & purification*
C117457:AGO1 protein, Arabidopsis; C471209:AGO4 protein, Arabidopsis; C521197:AGO6 protein, Arabidopsis; D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; D034741:RNA, Small Interfering; D034743:RNA-Induced Silencing Complex