Expression of 4 truncated fragments of Pasteurella multocida toxin and their immunogenicity.
Pasteurella multocida toxin (PMT) is a poor antigen that becomes more immunogenic after its native structure has been destroyed. In contrast, partially truncated PMT proteins, which are predicted to be good antigens when used as a vaccine, might be used to improve the control of atrophic rhinitis in pigs. In this study, 4 truncated PMT fragments were expressed in Escherichia coli, and those 4 fragments were inoculated into mice to produce the polyclonal antibodies. The results of an enzyme-linked immunosorbent assay (ELISA) revealed that #1 and #4 fragments were the most immunogenic. Immunized mice were subsequently challenged intraperitoneally with P. multocida type D. Five of the eight #1 fragment-immunized mice showed some protection against death and bacterial clearance. Pigs immunized with #1 fragment produced no or mild atrophic rhinitis (turbinate conchal score) after challenge, suggesting that this #1 fragment could be a good candidate for a subunit recombinant-type vaccine.
Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Jayoung
J
Seo
Hyoju
H
Pyo
Semi
S
Lee
Jaeil
J
Lee
Taejung
T
Kim
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007114:Immunization / Q000379:methods / Q000662:veterinary; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010326:Pasteurella Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology*; D010446:Peptide Fragments / Q000494:pharmacology*; D011897:Random Allocation; D012222:Rhinitis, Atrophic / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin; D010446:Peptide Fragments