Development of a diffraction imaging flow cytometer.
Diffraction images record angle-resolved distribution of scattered light from a particle excited by coherent light and can correlate highly with the 3D morphology of a particle. We present a jet-in-fluid design of flow chamber for acquisition of clear diffraction images in a laminar flow. Diffraction images of polystyrene spheres of different diameters were acquired and found to correlate highly with the calculated ones based on the Mie theory. Fast Fourier transform analysis indicated that the measured images can be used to extract sphere diameter values. These results demonstrate the significant potentials of high-throughput diffraction imaging flow cytometry for extracting 3D morphological features of cells.
Jacobs
Kenneth M
KM
Department of Physics, East Carolina University, Greenville, North Carolina 27858, USA.
Lu
Jun Q
JQ
Hu
Xin-Hua
XH
D003952:Diagnostic Imaging / Q000295:instrumentation; D004548:Elasticity; D004867:Equipment Design / Q000295:instrumentation; D005434:Flow Cytometry / Q000379:methods*; D005583:Fourier Analysis; D008027:Light; D016011:Normal Distribution; D055095:Optics and Photonics; D011137:Polystyrenes / Q000737:chemistry; D012542:Scattering, Radiation; D014867:Water / Q000737:chemistry
D011137:Polystyrenes; D014867:Water