A model of gene-environment interaction reveals altered mammary gland gene expression and increased tumor growth following social isolation.
Clinical studies have revealed that social support improves the outcome of cancer patients, whereas epidemiologic studies suggest that social isolation increases the risk of death associated with several chronic diseases. However, the precise molecular consequences of an unfavorable social environment have not been defined. To do so, robust, reproducible preclinical models are needed to study the mechanisms whereby an adverse environment affects gene expression and cancer biology. Because random assignment of inbred laboratory mice to well-defined social environments allows accurate and repeated measurements of behavioral and endocrine parameters, transgenic mice provide a preclinical framework with which to begin to determine gene-environment mechanisms. In this study, we found that female C3(1)/SV40 T-antigen mice deprived of social interaction from weaning exhibited increased expression of genes encoding key metabolic pathway enzymes in the premalignant mammary gland. Chronic social isolation was associated with up-regulated lipid synthesis and glycolytic pathway gene expression-both pathways are known to contribute to increased breast cancer growth. Consistent with the expression of metabolic genes in premalignant mammary tissue, isolated mice subsequently developed a significantly larger mammary gland tumors burden compared with group-housed mice. Endocrine evaluation confirmed that isolated mice developed a heightened corticosterone stress response compared with group-housed mice. Together, these transdisciplinary studies show for the first time that an adverse social environment is associated with altered mammary gland gene expression and tumor growth. Moreover, the identification of specific alterations in metabolic pathways gene expression favoring tumor growth suggests potential molecular biomarkers and/or targets (e.g., fatty acid synthesis) for preventive intervention in breast cancer.
Williams
J Bradley
JB
Department of Medicine, The University of Chicago, Chicago, Illinois.
Pang
Diana
D
Department of Medicine, The University of Chicago, Chicago, Illinois.
Delgado
Bertha
B
Department of Pathology, The University of Chicago, Chicago, Illinois.|The Institute of Mind and Biology, The University of Chicago, Chicago, Illinois.
Kocherginsky
Masha
M
Department of Health Studies, The University of Chicago, Chicago, Illinois.
Tretiakova
Maria
M
Department of Pathology, The University of Chicago, Chicago, Illinois.
Krausz
Thomas
T
Department of Pathology, The University of Chicago, Chicago, Illinois.
Pan
Deng
D
Department of Medicine, The University of Chicago, Chicago, Illinois.
He
Jane
J
Department of Medicine, The University of Chicago, Chicago, Illinois.
McClintock
Martha K
MK
The Institute of Mind and Biology, The University of Chicago, Chicago, Illinois.
Conzen
Suzanne D
SD
Department of Medicine, The University of Chicago, Chicago, Illinois.|The Institute of Mind and Biology, The University of Chicago, Chicago, Illinois.|Ben May Department for Cancer Research, The University of Chicago, Chicago, Illinois.
D000818:Animals; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000503:physiopathology / Q000523:psychology*; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D011230:Precancerous Conditions / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012934:Social Isolation