Intrathecal injection of GluR6 antisense oligodeoxynucleotides alleviates acute inflammatory pain of rectum in rats.
OBJECTIVE
To investigate whether the kainate (KA) receptor subunit GluR6 is involved in the acute inflammatory pain.


METHODS
Formalin was injected into the mucosa of rectum in Sprague-Dawley rats to induce visceral pain. The antisense oligodeoxynucleotides (ODNs) of GluR6 were injected once per day for 3 d before formalin injection, after which GluR6 protein level was examined by immunoblotting method. The change of visceral pain was also investigated.


RESULTS
The expression of GluR6 in the spinal cord of rats increased after the formalin injection. Moreover, pre-treatment of GluR6 antisense ODNs could suppress GluR6 expression in the spinal cord of rats and decrease the scores of visceral pain at 45 min following formalin injection.


CONCLUSIONS
Kainate receptor subunit GluR6 plays an important role in the visceral pain induced by injection of formalin into the wall of rectum. GluR6 may serve as a potential target for the treatment of acute inflammatory visceral pain.
Key Laboratory of Anesthesiology of Jiangsu Province, Xuzhou Medical College, Xuzhou 221002, China. lczhang@xzmc.edu.cn
Wei-Guang
WG
Zhang
Li-Cai
LC
Zhang
Zhen-Dan
ZD
Peng
Yin-Ming
YM
Zeng
D000700:Analgesics / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D005557:Formaldehyde; D015151:Immunoblotting; D007278:Injections, Spinal; D008297:Male; D009092:Mucous Membrane / Q000187:drug effects; D020319:Oligodeoxyribonucleotides, Antisense / Q000008:administration & dosage / Q000627:therapeutic use*; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D010147:Pain Measurement; D051381:Rats; D017207:Rats, Sprague-Dawley; D018092:Kainic Acid Receptors / Q000235:genetics / Q000378:metabolism*; D012007:Rectum; D013116:Spinal Cord / Q000378:metabolism; D013997:Time Factors; D000097806:GluK2 Kainate Receptor
D000700:Analgesics; D005557:Formaldehyde; D020319:Oligodeoxyribonucleotides, Antisense; D018092:Kainic Acid Receptors; D000097806:GluK2 Kainate Receptor