Experimental characterization and mitigation of specimen charging on thin films with one conducting layer.
Specimen charging may be one of the most significant factors that contribute to the high variability and generally low quality of images in cryo-electron microscopy. Understanding the nature of specimen charging can help in devising methods to reduce or even avoid its effects and thus improve the rate of data collection as well as the quality of the data. We describe a series of experiments that help to characterize the charging phenomenon, which has been termed the Berriman effect. The pattern of buildup and disappearance of the charge pattern has led to several suggestions for how to alleviate the effect. Experiments are described that demonstrate the feasibility of such charge mitigation.
Downing
Kenneth H
KH
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-0001, USA. KHDowning@lbl.gov
McCartney
M R
MR
Glaeser
Robert M
RM
D002244:Carbon; D020285:Cryoelectron Microscopy / Q000379:methods*; D004553:Electric Conductivity; D005240:Feasibility Studies; D006696:Holography; D010984:Platinum; D018717:Purple Membrane / Q000648:ultrastructure; D016610:Tissue Embedding / Q000379:methods*
D010984:Platinum; D002244:Carbon