Mass spectrometry as a tool for characterization of N,N-dialkylaminoethane-2-thiols--precursors and degradation products of chemical warfare agents.
N,N-dialkylaminoethane-2-thiols belong to the group of precursors and degradation products of chemical warfare agents (CWAs). These compounds were analyzed by means of electrospray ionization-multiple stage mass spectrometry (ion trap) and proposed fragments were confirmed by accurate mass measurement using a QqTOF system. The fragmentation pathways of studied compounds and the products of oxidation (formation of -S-S- linkage) were described. Some minor interesting processes, such as rearrangement of SH group, were observed and proved. A new microLC/MS method, based on ion-pairing chromatography, was developed. Trifluoroacetic acid was employed as an ion-pairing agent to increase the low retention of compounds of interest in the reverse-phase system. The technique was compared with the UPLC/MS method, allowing fast analysis of all the studied thiols as well as an explorative control of originated disulfides.
Papousková
Barbora
B
Faculty of Science, Department of Analytical Chemistry, Palacký University, Trída Svobody 8, 771 46 Olomouc, Czech Republic.
Bednár
Petr
P
Stýskala
Jakub
J
Hlavác
Jan
J
Barták
Petr
P
Lemr
Karel
K
D002619:Chemical Warfare Agents / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D002619:Chemical Warfare Agents; D004220:Disulfides; D013438:Sulfhydryl Compounds