Involvement of two rice ETHYLENE INSENSITIVE3-LIKE genes in wound signaling.
Ethylene and jasmonic acid (JA) have been proposed as key compounds for wound signaling in plants. In Arabidopsis, ETHYLENE INSENSITIVE3 (EIN3), which is an essential transcription factor for ethylene signaling, is regulated at the post-transcriptional level, while transcriptional regulation of EIN3 or EIN3-LIKE (EIL) genes has not been well documented. The expression of 6 rice EIL genes (OsEIL1-6) was analyzed and only OsEIL1 and 2 were found to be wound-inducible EIL. OsEIL2 was also induced by JA. Electrophoretic mobility shift assays showed that recombinant OsEIL1 and 2 proteins bound to specific DNA sequences that are recognized by a wound-inducible tobacco EIL. Accumulation of OsEIL1 and 2 transcripts reached a maximum at 1 and 0.5 h after wounding, respectively, and the corresponding DNA-binding activity in nuclear extracts of rice leaves was increased at 1 h after wounding. Candidates for OsEIL-target genes were selected by microarray analysis of wounded rice and by promoter sequence analyses of wound-inducible genes identified by microarray analysis. In OsEIL1- and/or 2-suppressed rice plants, the expression of at least four of 18 candidate genes analyzed was down-regulated. These results indicate the importance of inducible OsEILs in wound signaling in rice.
Hiraga
Susumu
S
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8518, Japan.
Sasaki
Katsutomo
K
Hibi
Tadaharu
T
Yoshida
Hitoshi
H
Uchida
Eiji
E
Kosugi
Shunichi
S
Kato
Takeshi
T
Mie
Takashi
T
Ito
Hiroyuki
H
Katou
Shinpei
S
Seo
Shigemi
S
Matsui
Hirokazu
H
Ohashi
Yuko
Y
Mitsuhara
Ichiro
I
D001483:Base Sequence; D001665:Binding Sites; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D015398:Signal Transduction / Q000235:genetics*; D013489:Suppression, Genetic; D014026:Nicotiana / Q000235:genetics
D002457:Cell Extracts; D018744:DNA, Plant; D010940:Plant Proteins