Three new species of bipolar budding yeasts of the genus Hanseniaspora and its anamorph Kloeckera isolated in Thailand.
In the course of a survey of yeast biodiversity in the natural substrates in Thailand, eight strains were found to represent three hitherto undescribed species of Hanseniaspora/Kloeckera. They were isolated from insect frass, flower, lichen, rotted fruit and rotted wood. Based on the morphological and physiological characteristics, and sequences of D1/D2 domain, six strains represent a single species of the genus Hanseniaspora, described as Hanseniaspora thailandica sp. nov. (type BCC 14938(T)=NBRC 104216(T)=CBS 10841(T)), and another strain as Hanseniaspora singularis sp. nov. (type BCC 15001(T)=NBRC 104214(T)=CBS 10840(T)). A further strain, which belongs to Kloeckera and does not produce ascospores, is described as Kloeckera hatyaiensis sp. nov. (type BCC 14939(T)=NBRC 104215(T)=CBS 10842(T)). Strains belonging to H. thailandica sp. nov. differed by 17-19 nucleotide substitutions from Hanseniaspora meyeri, the closest species. DNA reassociation between the two taxa showed 30-48% relatedness. Kloeckera hatyaiensis sp. nov. and H. singularis sp. nov. differed by eight and 16 nucleotide substitutions with one gap from the nearest species, Hanseniaspora clermontiae and Hanseniaspora valbyensis, respectively.
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand. sasitorn@biotec.or.th
Sasitorn
S
Jindamorakot
Shinya
S
Ninomiya
Savitree
S
Limtong
Wichien
W
Yongmanitchai
Manee
M
Tuntirungkij
Wanchern
W
Potacharoen
Kenji
K
Tanaka
Hiroko
H
Kawasaki
Takashi
T
Nakase
D000818:Animals; D001482:Base Composition; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D055321:Hanseniaspora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D007313:Insecta / Q000382:microbiology*; D055322:Kloeckera / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010944:Plants / Q000382:microbiology*; D012331:RNA, Fungal / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000166:cytology; D013785:Thailand; D014451:Ubiquinone / Q000032:analysis
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012339:RNA, Ribosomal, 28S; D014451:Ubiquinone