Transforming growth factor-beta3 expression up-regulates on cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate, in which the molecular etiology of the defect is poorly characterized. Recently, transforming growth factor-beta3 (TGF-beta3) has been indicated to play an essential role in the development of palatal shelves. In this developmental toxicity study, we investigated the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of TGF-beta3 in fetal mice. Pregnant C57BL/6 mice were exposed to corn oil or TCDD (32 microg/kg/day 64 microg/kg/day, per os) at embryonic day 10 (ED10), a drastic inhibition of palatal shelves was induced. By using RT-PCR (reverse transcription-polymerase chain reaction) and Western blot, the expressions of TGF-beta3 was investigated. We found that the expression of TGF-beta3 was gradually up-regulated in TCDD-treated group. These results suggest that cleft palate can be induced by TCDD exposure, the modification of TGF-beta3 is related to its pathogenesis.
Gan
Li-qiang
LQ
Department of Pediatric Surgery, Chongqing Children's Hospital, Chongqing Medical University, Chongqing, China.
Fu
Yue-xian
YX
Liu
Xing
X
Qiu
Lin
L
Wu
Sheng-de
SD
Tian
Xiao-fei
XF
Liu
Yan
Y
Wei
Guang-hui
GH
D000014:Abnormalities, Drug-Induced / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002972:Cleft Palate / Q000139:chemically induced* / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000187:drug effects; D007150:Immunohistochemistry; D051379:Mice; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053782:Transforming Growth Factor beta3 / Q000378:metabolism*; D015854:Up-Regulation
D000072317:Polychlorinated Dibenzodioxins; D053782:Transforming Growth Factor beta3