[The effect of vasopressin and its analogs on hyaluronan-synthase type 2 gene expression in Wistar and vasopressin-deficient Brattleboro rat kidney].
Effect of vasopressin and its analogs on the expression of HAS2 gene in different functional zones of Wistar and homozygous vasopressin-defficient Brattlboro rat kidneys was analyzed using RT-PCR method. It has been found that in the inner medulla of Wistar rat kidney the content of HAS2 mRNA depends on the level ofvasopressin in the blood: treatment by dDAVP (agonist of V2 receptor) suppressed the expression of HAS2, whereas lowered vasopressin secretion under hydration caused increase of HAS2 mRNA content. In the inner medulla of vasopressin-deficient Brattleboro rat kidney, exogenous arginin-vasopressin and its analog AVP-A (agonist of V2 and antagonist of V1a receptor) suppressed HAS2 expressin. It seems that U2 receptor takes part in hormonal regulation HA metabolism system. The age-related dynamics of HAS2 expression in the medulla were studied in 5, 10 and 30-days old rats of both strains. HAS2 gene expressed during the early period of postnatal life in the medulla and reached maximum on the 30th day. The comparison of the data obtained in Wistar and Brattleboro rats suggest that vasopressin suppresses HAS2 expression in the inner medulla of adult rat kidney and has no influence on the maturing of the HA synthesis enzyme system during postnatal development.
Ivanova
L N
LN
Kabilova
N O
NO
D000818:Animals; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology; D007684:Kidney Tubules / Q000187:drug effects / Q000201:enzymology; D009995:Osmosis; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011910:Rats, Brattleboro; D017208:Rats, Wistar; D017483:Receptors, Vasopressin / Q000819:agonists; D014667:Vasopressins / Q000235:genetics / Q000494:pharmacology*
D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D014667:Vasopressins; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; C491097:Has2 protein, rat; D000076002:Hyaluronan Synthases; D003894:Deamino Arginine Vasopressin