Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.
We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associated with processing film.
Khoury
Mitri K
MK
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA.
Parker
Ian
I
Aswad
Dana W
DW
D015153:Blotting, Western / Q000379:methods*; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008670:Metals / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors; D014962:X-Ray Film
D008670:Metals; D010087:Oxides