In situ immobilization of acid protease on mesoporous activated carbon packed column for the production of protein hydrolysates.
The mesoporous activated carbon (MAC) was used as a support material for in situ immobilization of acid protease (AP). The optimum temperature for the activities of both free and immobilized AP was found to be 50 degrees C. The catalytic efficiency of AP-MAC system has significantly been maintained for more than ten consecutive reaction cycles. The functional groups and surface morphology of the AP, MAC and AP-MAC were observed by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The production of protein hydrolysates was carried out from bovine serum albumin (BSA) using AP-MAC packed column and its properties were studied.
Ganesh Kumar
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A
Department of Biomedical Engineering, Sathyabama University, Rajiv Gandhi Road, Chennai 600 119, India. microganesh@yahoo.com
Perinbam
K
K
Kamatchi
P
P
Nagesh
N
N
Sekaran
G
G
D000818:Animals; D002417:Cattle; D002606:Charcoal / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism* / Q000648:ultrastructure; D016062:Porosity; D011492:Protein Hydrolysates / Q000096:biosynthesis*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D004800:Enzymes, Immobilized; D011492:Protein Hydrolysates; D002606:Charcoal; D012710:Serum Albumin, Bovine; D010447:Peptide Hydrolases