Polyvinylpyrrolidone- (PVP-) coated silver aggregates for high performance surface-enhanced Raman scattering in living cells.
A biocompatible and stable surface-enhanced Raman scattering (SERS) probe has been successfully synthesized through a simple route with silver aggregates. Polyvinylpyrrolidone (PVP), a biocompatible polymer, was utilized to control the aggregation process and improve the chemical stability of the aggregates. Extinction spectroscopy and TEM results show the aggregation degree and core-shell structure of the probe. It is found that when we employ 4-mercaptobenzoic acid (4MBA), crystal violet (CV), Rhodamine 6G (R6G) or 4,4'-bipyridine molecules as Raman reporters, the SERS signal from the proposed probe can remain at a high level under aggressive chemical environments, even after being incorporated into living cells. In comparison with the traditional probes without the PVP shell, the new ones exhibit strong surface-enhanced effects and low toxicity towards living cells. We demonstrate that the PVP-coated silver aggregates are highly SERS effective, for which the fabrication protocol is advantageous in its simplicity and reproducibility.
Tan
Xuebin
X
Advanced Photonics Center, Southeast University, Nanjing 210096, People's Republic of China.
Wang
Zhuyuan
Z
Yang
Jing
J
Song
Chunyuan
C
Zhang
Ruohu
R
Cui
Yiping
Y
D001565:Benzoates / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D003102:Colloids / Q000737:chemistry; D004705:Endocytosis; D005840:Gentian Violet / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D011205:Povidone / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance
D001565:Benzoates; D003102:Colloids; D011725:Pyridines; D012235:Rhodamines; D013438:Sulfhydryl Compounds; C026188:rhodamine 6G; C013594:4-mercaptobenzoate; D012834:Silver; D012965:Sodium Chloride; D012835:Silver Nitrate; D011205:Povidone; D005840:Gentian Violet; C034306:4,4'-bipyridyl