Effect of dimethyl sulfoxide on cell cycle synchronization of goldfish caudal fin derived fibroblasts cells.
Several studies have previously been conducted regarding cell cycle synchronization in mammalian somatic cells. However, limited work has been performed on the control of cell cycle stages in the somatic cells of fish. The aim of this study was to determine the cell cycle arresting effects of several dimethyl sulfoxide (DMSO) concentrations for different times on different cell cycle stages of goldfish caudal fin-derived fibroblasts. Results demonstrated that the cycling cells or control group (68.29%) yields significantly higher (p < 0.05) arrest in G0/G1 phase compared with the group treated for 24 h with different concentrations (0.5%, 1.0% or 1.5%) of DMSO (64.88%, 65.70%, 64.22% respectively). The cell cycle synchronization in the treatment of cells with 1.0% DMSO at 48 h (81.14%) was significantly higher than that in the groups treated for 24 h (76.82%) and the control group (77.90%). Observations showed that treatment of DMSO resulted in an increase in the proportion of cells at G0/G1 phase for 48 h of culture. However, high levels of apoptotic cells can be detected after 48 h of culture treated with 1% concentration of DMSO.
Choresca
C H
CH
Laboratory of Aquatic Animal Medicine, Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul, South Korea.
Koo
O J
OJ
Hong
S G
SG
Oh
H J
HJ
Gomez
D K
DK
Kim
J H
JH
Lee
B C
BC
Park
S C
SC
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary; D002453:Cell Cycle / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005121:Extremities; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D006054:Goldfish / Q000502:physiology*; D013997:Time Factors
D004121:Dimethyl Sulfoxide