Genetic analysis of meiotic recombination in Schizosaccharomyces pombe.
The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays ofintragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.
Smith
Gerald R
GR
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
D018929:Cell Culture Techniques; D005821:Genetic Techniques; D008540:Meiosis / Q000235:genetics*; D008954:Models, Biological; D011995:Recombination, Genetic / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development