Imaging nervous system activity.
This unit describes methods for loading ion- and voltage-sensitive dyes into neurons, with a particular focus on the spinal cord as a model system. In addition, we describe the use of these dyes to visualize neural activity. Although the protocols described here concern spinal networks in culture or an intact in vitro preparation, they can be, and have been, widely used in other parts of the nervous system.
Section on Nervous System Development and Plasticity, National Institute of Child Health and Human Development (NICHD), NIH, Bethesda, Maryland, USA.
Douglas R
DR
Fields
Neil
N
Shneider
George Z
GZ
Mentis
Michael J
MJ
O'Donovan
D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009415:Nerve Net / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009482:Neurophysiology / Q000379:methods*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes