Attovial-based antibody nanoarrays.
Antibody array-based technology is a powerful emerging tool in proteomics, but to enable global proteome analysis, antibody array layouts with even higher density has to be developed. To this end, we have further developed the first generation of a nanoarray platform, based on attoliter-sized vials, attovials, which we have characterized and used for the detection of complement factor C1q in human serum samples. Finally, we demonstrated proof-of-concept for individual functionalization of the attovials with a recombinant antibody.
Ellmark
Peter
P
Department of Immunotechnology, Lund University, Lund, Sweden.
Ghatnekar-Nilsson
Sara
S
Meister
André
A
Heinzelmann
Harry
H
Montelius
Lars
L
Wingren
Christer
C
Borrebaeck
Carl A K
CA
D000906:Antibodies / Q000276:immunology*; D015922:Complement C1q / Q000032:analysis* / Q000276:immunology; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D040901:Proteomics / Q000379:methods
D000906:Antibodies; D015922:Complement C1q