Adipokine expression in adipose tissue and in peripheral blood mononuclear cells in children Correlation with BMI and fatty acid content.
OBJECTIVE
Adipose tissue contributes in energy, lipid homeostasis and inflammation, through the adipokines it releases. Our aim was to study the associations between adipose tissue (AT) fatty acid content, adipokines' expression in AT and PBMCs and BMI in children.


METHODS
Thirty-one (17 male) healthy children aged 10.9+/-1.8years and of BMI 19.3+/-3.5kg/m(2) were enrolled. Adipokines (TNF-alpha, IL-6, leptin and visfatin) expression was quantified by real-time quantitative PCR in AT and PBMCs. Serum levels were measured by ELISA and fatty acids (FA) from AT by gas chromatography.


RESULTS
BMI was correlated with monounsaturated fatty acids (MUFAs) (beta=0.339, p=0.043), arachidonic (AA) (beta=0.576, p< or =0.001) and eicosapentaenoic acids (EPA) (beta=0.404, p=0.004) and negatively with stearic acid (beta=-0.577, p< or =0.001). TNF-alpha and visfatin expression from PBMCs were positively correlated with MUFAs (beta=0.271, p=0.027 and beta=0.214, p=0.020, respectively), n-9 fatty acids (beta=0.313, p=0.010 and beta=0.269 and p=0.024, respectively) and with docosahexaenoic acid (DHA) (beta=0.429, p=0.004 and beta=0.484, p< or =0.001, respectively), while negatively with the ratio n-6/n-3 (beta=-0.490, p=0.007 and beta=-0.374, p=0.044).


CONCLUSIONS
A series of FA molecules were correlated with children's BMI and with TNF-alpha and visfatin expression from PBMCs indicating that AT fatty acid content, might have a role, as a potential regulator of PBMCs inflammatory gene expression.
Dedoussis
George V
GV
Department of Nutrition-Dietetics, Harokopio University, 70 El. Venizelou St., 17671 Athens, Greece. dedousi@hua.gr
Kapiri
Alexandra
A
Kalogeropoulos
Nick
N
Samara
Anastasia
A
Dimitriadis
Dimitris
D
Lambert
Daniel
D
Pfister
Michele
M
Siest
Gerard
G
Visvikis-Siest
Sophie
S
D054392:Adipokines / Q000032:analysis* / Q000097:blood; D000273:Adipose Tissue / Q000737:chemistry*; D001773:Blood Cells; D001823:Body Composition; D015992:Body Mass Index; D002648:Child; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000032:analysis / Q000097:blood; D020738:Leptin / Q000032:analysis / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000032:analysis / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000097:blood
D054392:Adipokines; D005227:Fatty Acids; D015850:Interleukin-6; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha; D054409:Nicotinamide Phosphoribosyltransferase