Effects of nutrient pre-exposure on atrazine toxicity to Vallisneria americana Michx. (wild celery).
Accelerated eutrophication is common to many freshwater and marine environments and often co-occurs with the presence of anthropogenic chemicals. However, the toxic effects of common chemical stressors such as herbicides in the presence of elevated nutrients are not well understood for most aquatic flora, particularly vascular species. To provide insight, field-collected Vallisneria americana Michx. (wild celery) were sequentially exposed to three nutrient concentrations for 3 months and then to nominal 11 and 110 microg L(-1) atrazine for 96 h. Nutrient concentrations (combined NH(4)(+), NO(2)(-), NO(3)(-), PO(4)(-)) were based on ambient concentrations in the St. Johns River (FL) and ranged from 0.013 to 0.668 mg L(-1). Nutrient pretreatment potentiated the toxicity of atrazine as determined by chlorophyll fluorescence activity. Electron transport rates (ETR) were significantly less (48-59%) for plants pretreated with low and ambient nutrient levels in the presence of an average of 107.5-128.1 microg L(-1) atrazine. Significant ETR reductions were also observed for plants exposed to an average of 11.4 microg L(-1) atrazine after exposure to nutrients three times the ambient concentration in the St. Johns River. The results indicate the importance of considering the presence of nutrients in chemical hazard assessments, particularly for phytotoxicants and nontarget vascular plants.
Dantin
Darrin D
DD
Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, USEPA, 1 Sabine Island Drive, Gulf Breeze, FL 32561, USA. dantin.darrin@epa.gov
Boustany
Ronald G
RG
Lewis
Michael A
MA
Jordan
Stephen J
SJ
Moss
Rebecca F
RF
Michot
Thomas C
TC
D001280:Atrazine / Q000633:toxicity*; D004579:Electron Transport / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D031321:Hydrocharitaceae / Q000187:drug effects* / Q000378:metabolism; D009566:Nitrates / Q000494:pharmacology; D009573:Nitrites / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D006540:Herbicides; D009566:Nitrates; D009573:Nitrites; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D014874:Water Pollutants, Chemical; D001280:Atrazine