A bone-like nano-hydroxyapatite/collagen loaded injectable scaffold.
From a clinical perspective, the use of injectable scaffolds is very attractive as it minimizes patient discomfort, risk of infection, scar formation and the cost of treatment. Bone refers to a family of materials that are constructed by mineralized collagen fibrils. The main objective of this research was to develop a bone-like nano-hydroxyapatite/collagen (nHAC) loaded chitosan (C)/beta-glycerophosphate (GP) injectable scaffold. The feasibility of developing a thermo-sensitive and injectable chitosan solution in the presence of nHAC was demonstrated. Bone-marrow-derived messenchymal stem cells (MSCs) were used to measure the cell proliferation of C/GP/nHAC scaffolds based on the cell count kit-8 (CCK-8) assay. It was found that MSCs proliferated normally with the C/GP/nHAC composite scaffolds. The C/GP/nHAC composite scaffolds developed in this study exhibited good injectability, thermo-irreversible properties and solidified under mild conditions. No more than 0.02 g ml(-1) of nHAC filler was required to form a non-decaying hydrogel.
Department of Materials Science and Engineering, State Key Laboratory of New Ceramics and Fine Processing, Tsinghua University, Beijing 100084, People's Republic of China.
Zhi
Z
Huang
Jing
J
Tian
Bo
B
Yu
Yong
Y
Xu
Qingling
Q
Feng
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D018786:Bone Substitutes / Q000008:administration & dosage* / Q000138:chemical synthesis*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000008:administration & dosage / Q000737:chemistry*; D017886:Durapatite / Q000008:administration & dosage / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D011817:Rabbits
D018786:Bone Substitutes; D003094:Collagen; D017886:Durapatite