Rapid identification of Propionibacterium acnes from blood cultures by fluorescence in situ hybridization.
A newly designed probe for rapid identification of Propionibacterium acnes by fluorescence in situ hybridization was evaluated using 111 isolates from subculture and showed 100% sensitivity and specificity. A sensitivity of 95% and a specificity of 100% were achieved with direct application on 55 blood cultures containing Gram-positive rods.
Poppert
Sven
S
Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany. poppert@bni-hamburg.de
Riecker
Melanie
M
Essig
Andreas
A
D016470:Bacteremia / Q000175:diagnosis; D001769:Blood / Q000382:microbiology*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D011425:Propionibacterium acnes / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes