Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample.
We have developed and validated quantitative ELISAs for human angiopoietin-like (ANGPTL)3 and 4 and correlated their serum levels with parameters of lipid and carbohydrate metabolism. For this study, we used a random subsample of the Health 2000 Health Examination Survey consisting of 125 men and 125 women, aged 30-94 years. The anthropometric and biochemical parameters of subjects were characterized in detail. ANGPTL 3 and 4 levels were determined using the developed ELISAs. The intra- and inter-assay coefficients of variation for the assays were less than 15%. The average serum concentration of ANGPTL3 was 368 +/- 168 ng/ml (mean +/- SD) and for ANGPTL4 it was 18 +/- 23 ng/ml (mean +/- SD). ANGPTL4 serum levels displayed high variability between individuals ranging from 2 to 158 ng/ml. In post-heparin plasma, both ANGPTL 3 and 4 were increased. Low levels of ANGPTL3 were associated with decreased HDL-cholesterol and increased triglyceride levels. ANGPTL4 levels were positively correlated with FFAs (P = 0.044) and waist-hip ratio (P = 0.016). The developed ELISAs will be important tools to clarify the role of ANGPTL 3 and 4 in human energy metabolism and partitioning of triglycerides between sites of storage (adipose tissue) and oxidation (skeletal and cardiac muscle).
Robciuc
Marius R
MR
Department of Chronic Disease Prevention, National Institute for Health and Welfare, Public Health Genomics Research Unit, Biomedicum, Helsinki, Finland.
Tahvanainen
Esa
E
Jauhiainen
Matti
M
Ehnholm
Christian
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000091303:Angiopoietin-Like Protein 3; D000075803:Angiopoietin-Like Protein 4; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005387:Finland; D006306:Health Surveys; D006493:Heparin / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D050177:Overweight / Q000097:blood; D015203:Reproducibility of Results; D012727:Sex Characteristics; D013997:Time Factors; D049629:Waist-Hip Ratio
C479896:ANGPTL3 protein, human; C474772:ANGPTL4 protein, human; D000091303:Angiopoietin-Like Protein 3; D000075803:Angiopoietin-Like Protein 4; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins; D008055:Lipids; D006493:Heparin