title: An immunohistochemical study of the myenteric plexus in idiopathic achalasia.
abstract: BACKGROUND
  Achalasia is a primary esophageal motor disorder characterized by degenerative changes of the myenteric plexus. The pathophysiologic abnormalities may be the final result of several intermeshing mechanisms, and more than one single factor may cause the motor abnormalities.
  
  
  OBJECTIVE
  To report our experience in investigating the myenteric plexus of achalasia patients undergoing esophagomyotomy.
  
  
  METHODS
  Tissue samples from 12 patients undergoing Heller myotomy for achalasia were evaluated and compared with esophageal tissue specimens from 7 controls. Enteric neurons and interstitial cells of Cajal (ICC) were assessed by immunohistochemical methods, and the presence of vasoactive intestinal polypeptide ergic fibers and of CD3 lymphocytes. The possible presence of herpesvirus was also assessed by immunohistochemistry, whereas that of papillomavirus was assessed by in-situ hybridization.
  
  
  RESULTS
  Compared with controls, achalasia patients displayed a significant decrease of both enteric neurons and ICC. Immunoreactivity for vasoactive intestinal polypeptide was completely absent in each patient. CD3 staining disclosed myenteric plexitis in 5 (42%) patients; no control patient had plexitis. All patients were completely negative for the presence of both herpes simplex virus and human papillomavirus.
  
  
  CONCLUSIONS
  The enteric nervous system of the lower esophageal sphincter area is impaired in patients with "idiopathic achalasia," and the abnormalities involve ICC and neurons in many patients. The triggering factors for these abnormalities are, however, still unknown.
authors.affiliation: 2nd Department of Pathology, Spedali Civili, Brescia, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Villanacci
authors.forename: Vito
authors.initials: V
authors.lastname: Annese
authors.forename: Antonello
authors.initials: A
authors.lastname: Cuttitta
authors.forename: Simona
authors.initials: S
authors.lastname: Fisogni
authors.forename: Gerardo
authors.initials: G
authors.lastname: Scaramuzzi
authors.forename: Ermelinda
authors.initials: E
authors.lastname: De Santo
authors.forename: Nadia
authors.initials: N
authors.lastname: Corazzi
authors.forename: Gabrio
authors.initials: G
authors.lastname: Bassotti
mesh_terms: D000328:Adult; D000368:Aged; D017252:CD3 Complex / Q000378:metabolism; D004931:Esophageal Achalasia / Q000276:immunology / Q000503:physiopathology; D004947:Esophagus / Q000276:immunology / Q000503:physiopathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D056885:Interstitial Cells of Cajal / Q000378:metabolism / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000166:cytology; D009197:Myenteric Plexus / Q000276:immunology / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
chemical_list: D017252:CD3 Complex; D014660:Vasoactive Intestinal Peptide