Simultaneous expression of glutathione, thioredoxin-1, and their reductases in nerve transected hypoglossal motor neurons of rat.
Anti-oxidative stress responses are crucial for the survival of nerve-injured motor neurons. Herein, we examined changes in expression of glutathione reductase (GSHr), thioredoxins (TRX1 and TRX2), and thioredoxin reductases (TRXr1 and TRXr2), important constituents of anti-oxidative pathways, following rat hypoglossal nerve transection. RT-PCR and in situ hybridization demonstrated that GSHr, TRX1, and TRXr1 mRNAs were significantly up-regulated during the first few weeks in nerve-injured motor neurons, while TRX2 and TRXr2 mRNAs were unchanged throughout 8 weeks after nerve transection. The up-regulation of GSH, GSHr, TRX1, and TRXr1 proteins in injured neurons was confirmed by immunohistochemical analysis. Western blotting also demonstrated up-regulation of GSHr, TRX1, and TRXr1 in injured neurons. These data suggest that the two major redox systems, GSH/GSHr and TRX1/TRXr1, are simultaneously activated in injured neurons, and likely provide protection of injured neurons against oxidative stress.
Hama
Isuzu
I
Department of Anatomy and Neurobiology, Osaka City University, Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
Nakagomi
Saya
S
Konishi
Hiroyuki
H
Kiyama
Hiroshi
H
D000818:Animals; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007002:Hypoglossal Nerve / Q000201:enzymology / Q000503:physiopathology*; D061228:Hypoglossal Nerve Injuries; D008297:Male; D009046:Motor Neurons / Q000201:enzymology / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D054481:Thioredoxin Reductase 1 / Q000378:metabolism; D054482:Thioredoxin Reductase 2 / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism; D013997:Time Factors
D012333:RNA, Messenger; C495697:Txn1 protein, rat; C516113:Txn2 protein, rat; D013879:Thioredoxins; D005980:Glutathione Reductase; D054481:Thioredoxin Reductase 1; D054482:Thioredoxin Reductase 2; C516126:Txnrd1 protein, rat; C516129:Txnrd2 protein, rat; D005978:Glutathione