title: Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment.
abstract: OBJECTIVE
  Although the role of bacteria in the etiology of periodontitis is well established, it has been suggested that herpetic viruses could contribute to the initiation and progression of this disease. The aim of this study was to determine the prevalence of human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) and herpes simplex virus (HSV) in gingival crevicular fluid (GCF) samples obtained from periodontally healthy, gingivitis and periodontitis patients. In addition, the effect of periodontal treatment (scaling and root planing) on the persistence of herpetic viruses was evaluated in a sub-group of patients suffering from chronic periodontitis.
  
  
  METHODS
  The presence of viruses in GCF samples was assessed by a nested PCR amplification technique. The persistence of viruses in periodontal sites was evaluated following a scaling and root planing therapy.
  
  
  RESULTS
  A statistically significant higher prevalence of HCMV was observed in periodontitis patients as compared to healthy control subjects (35 vs. 8%, respectively; P = 0.0377). A trend for a higher prevalence of HSV was also noted in the periodontitis group, in comparison with healthy control subjects. In addition, a higher prevalence of HCMV was associated with deep periodontal pockets in subjects suffering from periodontitis. In the sub-group of periodontitis patients, periodontal therapy resulted in the elimination (HCMV and EBV) or reduction (HSV) of the herpetic viruses.
  
  
  CONCLUSIONS
  This study showed that the prevalence of HCMV and HSV viruses in GCF is higher in patients suffering from periodontitis compared to periodontally healthy subjects, and that the prevalence of HCMV is higher in deep periodontal pockets. It also brought evidences that periodontal therapy may be associated with virus elimination in diseased sites.
authors.affiliation: Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada. Daniel.Grenier@greb.ulaval.ca
authors.forename: G
authors.initials: G
authors.lastname: Grenier
authors.forename: G
authors.initials: G
authors.lastname: Gagnon
authors.forename: D
authors.initials: D
authors.lastname: Grenier
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003587:Cytomegalovirus / Q000302:isolation & purification; D012534:Dental Scaling; D005260:Female; D005883:Gingival Crevicular Fluid / Q000821:virology*; D005891:Gingivitis / Q000382:microbiology* / Q000628:therapy; D006564:Herpesviridae / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000382:microbiology* / Q000628:therapy; D018139:Simplexvirus / Q000302:isolation & purification; D055815:Young Adult