Proline and glycinebetaine confer cadmium tolerance on tobacco bright yellow-2 cells by increasing ascorbate-glutathione cycle enzyme activities.
Cadmium (Cd) stress significantly decreased membrane integrity and impaired the ascorbate (ASC)-glutathione (GSH) cycle in tobacco Bright Yellow-2 cells. Exogenous application of proline and glycinebetaine (betaine) significantly restored the membrane integrity and increased the activities of ASC-GSH cycle enzymes under Cd stress without maintenance of the rich ASC or GSH pools. Moreover, proline offered more efficient protection against Cd stress than betaine.
Islam
Mohammad Muzahidul
MM
Graduate School of Natural Science and Technology, Okayama University, Japan.
Hoque
Md Anamul
MA
Okuma
Eiji
E
Jannat
Rayhanur
R
Banu
Mst Nasrin Akhter
MN
Jahan
Md Sarwar
MS
Nakamura
Yoshimasa
Y
Murata
Yoshiyuki
Y
D001205:Ascorbic Acid / Q000378:metabolism*; D001622:Betaine / Q000494:pharmacology*; D002104:Cadmium / Q000633:toxicity*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D011392:Proline / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism
D002104:Cadmium; D001622:Betaine; D011392:Proline; D006861:Hydrogen Peroxide; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid