title: Herpetomonas trimorpha sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera).
abstract: Monoxenous trypanosomatid Herpetomonas trimorpha sp. nov. was isolated from the digestive tract of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera). This species forms three distinct morphotypes in culture: the microflagellate promastigote, the small promastigote and the long promastigote. The last form is unique for the newly described species. Phylogenetic analyses of SSU rRNA and glycosomal glyceraldehyde phosphate dehydrogenase genes showed that H. trimorpha sp. nov. is the closest relative of Herpetomonas ztiplika, another monoxenous trypanosomatid isolated from biting midges. However, morphological and randomly amplified polymorphic DNA analyses confirmed that H. trimorpha sp. nov. is distinct from H. ztiplika.
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Zídková
authors.affiliation: Department of Zoology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Cepicka
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Votýpka
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
authors.forename: Milena
authors.initials: M
authors.lastname: Svobodová
mesh_terms: D000818:Animals; D002519:Ceratopogonidae / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D041981:Gastrointestinal Tract / Q000469:parasitology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D014351:Trypanosomatina / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D015800:Protozoan Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases