title: Quantitative relationship between maximum growth rates and the intracellular pattern of alpha-esterase and beta-esterase activity of leguminous infecting bacteria.
abstract: Sixteen strains belonging to three families of the Rhizobiales order (Bradyrhizobiaceae, Phyllobacteriaceae and Rhizobiaceae) were evaluated according their specific growth rates (micro) and the activity of intracellular alpha-esterase and beta-esterase isoenzymes. The average esterase activity of 48 isoenzymes assayed belonging to five strains with low (micro(max) = 0.08-0.12 h(-1)), four medium (micro(max) = 0.13-0.22 h(-1)) and seven high (micro(max) = 0.24-0.28 h(-1)) growth rate values were 22.1 +/- 4.3; 8.7 +/- 2.2 and 3.9 +/-1.7 U g(-1) respectively. An inversely proportional relationship between the activity of the whole pattern of esterases and micro(max) was found. Our results illustrate a feature of intracellular esterases, ascribable in a variety of cellular functions, which might be related to characteristics micro(max) of legume infecting bacteria.
authors.affiliation: Depto. de Química, CONICET, (FCEyN) Universidad Nacional de La Pampa (UNLPam), (6300) Santa Rosa, La Pampa, Argentina.
authors.forename: Alicia E
authors.initials: AE
authors.lastname: Grassano
authors.forename: Ana L
authors.initials: AL
authors.lastname: Ronchi
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Garcia
authors.forename: Laura
authors.initials: L
authors.lastname: Mazzaferro
authors.forename: Javier D
authors.initials: JD
authors.lastname: Breccia
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development*; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000378:metabolism*; D007887:Fabaceae / Q000382:microbiology*; D042541:Intracellular Space / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*
chemical_list: D007527:Isoenzymes; D004950:Esterases