Sulfated modification and immunomodulatory activity of water-soluble polysaccharides derived from fresh Chinese persimmon fruit.
In this study, three kinds of chemically sulfated polysaccharides (PFP-S) were derived from a water-soluble polysaccharide of persimmon fruit with chlorosulfonic acid-pyridine method. Relationship between the degree of substitution and immunomodulatory activity of PFP-S was examined with the splenocytes experiment. The results showed that the splenocytes-activating activity was significantly enhanced by PFP-S in all the groups compared with control group (P<0.01). PFP-SII exhibited the most potent splenocytes-activating activity by increased cytokine production and NO release. It also suggested that the sulfate groups and molecular weight of polysaccharides are key factors to regulate the immunomodulatory activities.
Zhang
Yali
Y
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xianning Road 28#, Xi'an 710049, Shaanxi Province, PR China.
Lu
Xiaoyun
X
Zhang
Yuning
Y
Qin
Liguo
L
Zhang
Jianbao
J
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002681:China; D029788:Diospyros / Q000737:chemistry*; D005260:Female; D005638:Fruit / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D007155:Immunologic Factors / Q000276:immunology* / Q000494:pharmacology; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012995:Solubility / Q000187:drug effects; D013055:Spectrophotometry, Infrared; D013154:Spleen / Q000166:cytology; D013431:Sulfates / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014867:Water
D007155:Immunologic Factors; D007376:Interleukin-2; D011134:Polysaccharides; D012333:RNA, Messenger; D013431:Sulfates; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D009569:Nitric Oxide