title: Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis.
abstract: A new 3-min rapid dot immunogold filtration assay (DIGFA) for serodiagnosis of human cystic and alveolar echinococcosis was developed using four native antigen preparations: crude and partially purified hydatid cyst fluid extracts from Echinococcus granulosus (EgCF and AgB), E. granulosus protoscolex extract (EgP) and Echinococcus multilocularis metacestode antigen (Em2). The overall sensitivity of DIGFA in a hospital diagnostic setting was 80.7% for human cystic echinococcosis (CE) (n=857) and 92.9% for human alveolar echinococcosis (AE) (n=42). Highest specificity was 93.4% with AgB extract for CE, and 90.3% with Em2 antigen for AE when CE versus AE cross-reactivity was excluded. Anti-AgB antibodies were present in 35.5% of AE cases and anti-Em2 in 7.4% of CE cases. In endemic communities in northwest China screened for echinococcosis, the sensitivity of DIGFA ranged from 71.8% to 90.7% in comparison to abdominal ultrasound; specificity for CE using AgB was 94.6% and for AE using Em2 was 97.1%. This simple eye-read rapid test can be used for both clinical diagnostic support, as well as in conjunction with ultrasound for mass screening in endemic CE and AE areas.
authors.affiliation: Xinjiang Hydatid Clinical Research Institute, First Teaching Hospital of Xinjiang Medical University, No.1 Liyushan RD, Urumqi 830000, Xinjiang, China. feng_xh_cn@yahoo.com
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
authors.forename: Xudong
authors.initials: X
authors.lastname: Ma
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
authors.forename: Xinwei
authors.initials: X
authors.lastname: Qi
authors.forename: Helen
authors.initials: H
authors.lastname: Bradshaw
authors.forename: Dominique
authors.initials: D
authors.lastname: Vuitton
authors.forename: Philip S
authors.initials: PS
authors.lastname: Craig
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D004443:Echinococcosis / Q000175:diagnosis* / Q000469:parasitology; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000469:parasitology; D048209:Echinococcus granulosus / Q000276:immunology / Q000302:isolation & purification*; D048210:Echinococcus multilocularis / Q000276:immunology / Q000302:isolation & purification*; D005374:Filtration; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth