title: Solution conformation of C-linked antifreeze glycoprotein analogues and modulation of ice recrystallization.
abstract: Antifreeze glycoproteins (AFGPs) are a unique class of proteins that are found in many organisms inhabiting subzero environments and ensure their survival by preventing ice growth in vivo. During the last several years, our laboratory has synthesized functional C-linked AFGP analogues (3 and 5) that possess custom-tailored antifreeze activity suitable for medical, commercial, and industrial applications. These compounds are potent inhibitors of ice recrystallization and do not exhibit thermal hysteresis. The current study explores how changes in the length of the amide-containing side chain between the carbohydrate moiety and the polypeptide backbone in 5 influences ice recrystallization inhibition (IRI) activity. Analogue 5 (n = 3, where n is the number of carbons in the side chain) was a potent inhibitor of ice recrystallization, while 4, 6, and 7 (n = 4, 2, and 1, respectively) exhibited no IRI activity. The solution conformation of the polypeptide backbone in C-linked AFGP analogues 4-7 was examined using circular dichroism (CD) spectroscopy. The results suggested that all of the analogues exhibit a random coil conformation in solution and that the dramatic increase in IRI activity observed with 5 is not due to a change in long-range solution conformation. Variable-temperature (1)H NMR studies on truncated analogues 26-28 failed to elucidate the presence of persistent intramolecular bonds between the amide in the side chain and the peptide backbone. Molecular dynamics simulations performed on these analogues also failed to show persistent intramolecular hydrogen bonds. However, the simulations did indicate that the side chain of IRI-active analogue 26 (n = 3) adopts a unique short-range solution conformation in which it is folded back onto the peptide backbone, orienting the more hydrophilic face of the carbohydrate moiety away from the bulk solvent. In contrast, the solution conformation of IRI-inactive analogues 25, 27, and 28 had fully extended side chains, with the carbohydrate moiety being exposed to bulk solvent. These results illustrate how subtle changes in conformation and carbohydrate orientation dramatically influence IRI activity in C-linked AFGP analogues.
authors.affiliation: Department of Chemistry, D'Iorio Hall, 10 Marie Curie, University of Ottawa, Ottawa, ON, Canada K1N 6N5.
authors.forename: Roger Y
authors.initials: RY
authors.lastname: Tam
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Rowley
authors.forename: Ivan
authors.initials: I
authors.lastname: Petrov
authors.forename: Tianyi
authors.initials: T
authors.lastname: Zhang
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Afagh
authors.forename: Tom K
authors.initials: TK
authors.lastname: Woo
authors.forename: Robert N
authors.initials: RN
authors.lastname: Ben
mesh_terms: D021301:Antifreeze Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D003460:Crystallization; D007053:Ice; D009682:Magnetic Resonance Spectroscopy; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation
chemical_list: D021301:Antifreeze Proteins; D007053:Ice