Rapid data analysis method for differential mobility cytometry.
Differential mobility cytometry (DMC) has recently been established as a powerful method to capture cells and study adhesion processes. DMC uses an oscillation system and cell affinity chromatography to monitor cells as they adhere to a surface. In the past, differential images had to be created individually which limited the throughput of the method. A new method to create differential images is presented. The method involves the subtraction of short movies from each other to create a stack of differential images that can be easily analyzed. In the future, this method will make DMC more accessible and improve throughput.
Reif
Randall D
RD
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.
Wang
Kelong
K
Pappas
Dimitri
D
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D006801:Humans