Ultrastructural evidence of amyloid beta-induced autophagy in PC12 cells.
Herein we demonstrate that PC12 cells overexpress human amyloid beta precursor protein bearing double Swedish mutation(AbetaPPsw), showing the phenotype characteristic for Alzheimer's disease (AD). Examination of cells at ultrastructural level revealed the intracellular presence of peptide aggregates. Furthermore, autophagy induction was found to be a hallmark of amyloid beta-induced cytotoxicity. Importantly, autophagic vacuoles were co-localized within amyloid beta (Abeta) deposits. This suggests the involvement of autophagy in amyloid beta-elicited cell degeneration.
Pajak
Beata
B
Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland. bepaj@wp.pl
Songin
Martyna
M
Strosznajder
Joanna B
JB
Orzechowski
Arkadiusz
A
Gajkowska
Barbara
B
D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001343:Autophagy / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D009410:Nerve Degeneration / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D016716:PC12 Cells; D051381:Rats; D014162:Transfection
D016564:Amyloid beta-Protein Precursor