Characterization of newly established bovine intestinal epithelial cell line.
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.
Miyazawa
Kohtaro
K
Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Japan. kohtaroh.miyazawa@yale.edu
Hondo
Tetsuya
T
Kanaya
Takashi
T
Tanaka
Sachi
S
Takakura
Ikuro
I
Itani
Wataru
W
Rose
Michael T
MT
Kitazawa
Haruki
H
Yamaguchi
Takahiro
T
Aso
Hisashi
H
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology*; D051379:Mice