title: HLA-DQA1 gene expression profiling in oligoarticular JIA.
abstract: Polymorphisms in the upstream regulatory region of the HLA class II DQA1 gene are currently defined by 10 different alleles. Two of them carrying a Y-box mutation are associated with susceptibility to oligoarticular juvenile idiopathic arthritis (OA-JIA). We investigated allele-dependent differences in HLA-DQA1 gene expression in OA-JIA patients. In cells from affected joints compared to peripheral blood, gene expression of HLA-DRA as well as total HLA-DQA1 was significantly upregulated. Differential analyses of HLA-DQA1 allelic expression showed DQA1*02 and *04 to be comparatively increased. Intra-articular upregulation of HLA-DQA1 was predominantly observed for the OA-JIA associated allele HLA-DQA1*04. Nevertheless, the Y-box mutation of the disease-associated allele DQA1*0401 was not a common denominator for expression behaviour.
authors.affiliation: Department of Medicine III, Institute for Clinical Immunology, Friedrich Alexander University, Erlangen Nuremberg, Germany.
authors.forename: J P
authors.initials: JP
authors.lastname: Haas
authors.forename: M
authors.initials: M
authors.lastname: Metzler
authors.forename: C
authors.initials: C
authors.lastname: Frank
authors.forename: R
authors.initials: R
authors.lastname: Haefner
authors.forename: R
authors.initials: R
authors.lastname: Wassmuth
mesh_terms: D001171:Arthritis, Juvenile / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen