A general life-death selection strategy for dissecting protein functions.
Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.
Ear
Po Hien
PH
Département de Biochimie, Université de Montréal, Montréal, Québec, Canada.
Michnick
Stephen W
SW
D043525:Cytosine Deaminase / Q000235:genetics*; D005816:Genetic Complementation Test / Q000379:methods; D010446:Peptide Fragments / Q000378:metabolism; D025941:Protein Interaction Mapping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development
D010446:Peptide Fragments; D043525:Cytosine Deaminase