Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine.
A multianalyte lateral-flow immunochromatographic technique using colloidal gold-labeled polyclonal antibodies was developed for the rapid simultaneous detection of clenbuterol and ractopamine. The assay procedure could be accomplished within 5 min, and the results of this qualitative one-step assay were evaluated visually according to whether test lines appeared or not. When applied to the swine urines, the detection limit and the half maximal inhibitory concentration (IC(50)) of the test strip under an optical density scanner were calculated to be 0.1 +/- 0.01 ng mL(-1) and 0.1 +/- 0.01 ng mL(-1), 0.56 +/- 0.08 ng mL(-1), and 0.71 +/- 0.06 ng mL(-1), respectively, the cut-off levels with the naked eye of 1 ng mL(-1) and 1 ng mL(-1) for clenbuterol and ractopamine were observed. Parallel analysis of swine urine samples with clenbuterol and ractopamine showed comparable results obtained from the multianalyte lateral-flow test strip and GC-MS. Therefore, the described multianalyte lateral-flow test strip can be used as a reliable, rapid, and cost-effective on-site screening technique for the simultaneous determination of clenbuterol and ractopamine residues in swine urine.
Zhang
Ming-Zhou
MZ
Institute of Bio-safety and Food Science, College of Life Science, China Jiliang University, Hangzhou, 310018, People's Republic of China. zmzcjlu@cjlu.edu.cn
Wang
Min-Zi
MZ
Chen
Zong-Lun
ZL
Fang
Jie-Hong
JH
Fang
Mei-Ming
MM
Liu
Jun
J
Yu
Xiao-Ping
XP
D000318:Adrenergic beta-Agonists / Q000276:immunology / Q000652:urine*; D000818:Animals; D002976:Clenbuterol / Q000276:immunology / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D017979:Gold Colloid / Q000737:chemistry* / Q000276:immunology*; D007118:Immunoassay / Q000379:methods*; D010627:Phenethylamines / Q000276:immunology / Q000652:urine*; D011934:Reagent Strips; D013552:Swine
D000318:Adrenergic beta-Agonists; D017979:Gold Colloid; D010627:Phenethylamines; D011934:Reagent Strips; C060897:ractopamine; D002976:Clenbuterol