title: Binding and elution behavior of proteins on strong cation exchangers.
abstract: This work provides a broad survey of binding and elution behavior of proteins on strong cation exchangers. Four proteins comprising two monoclonal antibodies, lysozyme, and cytochrome c were used as models in the investigation. Seven chromatography resins with different base matrices were compared. Dynamic binding capacity as a function of salt concentration was examined for a monoclonal antibody and lysozyme. Elution behavior as a function of gradient slope was modeled to determine the characteristic charge, essentially a measure of the number of sites involved in binding, for each protein on each resin. Trends with respect to dynamic binding capacity and elution behavior are analyzed and discussed.
authors.affiliation: Pfizer Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA.
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Pabst
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Suda
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Thomas
authors.forename: Paul
authors.initials: P
authors.lastname: Mensah
authors.forename: Natraj
authors.initials: N
authors.lastname: Ramasubramanyan
authors.forename: Mark E
authors.initials: ME
authors.lastname: Gustafson
authors.forename: Alan K
authors.initials: AK
authors.lastname: Hunter
mesh_terms: D000818:Animals; D002411:Cation Exchange Resins / Q000737:chemistry*; D002460:Cell Line; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D006736:Horses; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*
chemical_list: D002411:Cation Exchange Resins; D011506:Proteins