Structural analysis of an immunoenhancing heteropolysaccharide isolated from the green (unripe) fruits of Solenum melongena (Brinjal).
A water-soluble polysaccharide (SMPS) isolated from the aqueous extract of the green (unripe) fruits of Solenum melongena (Brinjal) contains D-galactose, D-methyl galacturonate, 3-O-acetyl D-methyl galacturonate, and L-arabinose in a molar proportion of nearly 1:1:1:1. Structural investigation of the polysaccharide was carried out using total acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above experiments the structure of the repeating unit of the polysaccharide (SMPS) was established as -->5)-alpha-L-Araf-(1-->4)-beta-D-Galp-(1-->2)-alpha-D-3-O-Ac-GalpA6Me-(1-->2)alpha-D-galpA6Me-(1--> This molecule showed splenocyte and thymocyte activations.
Ojha
Arnab K
AK
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Chandra
Krishnendu
K
Ghosh
Kaushik
K
Bhunia
Bibhas
B
Maiti
Tapas K
TK
Islam
Syed S
SS
D002240:Carbohydrate Sequence; D005638:Fruit / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D032323:Solanum melongena / Q000737:chemistry*
D011134:Polysaccharides