title: Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates.
abstract: Cryptococcus neoformans was grown in 96-well microtiter plates sealed by foil which is less than 0.01 % permeable to oxygen. On day 14 of the cultivation, we observed peculiar clusters of small droplike daughter cells arranged around < or = 4 % of mother cells. The fact that most of the other cells had died indicates that few cells had been able to survive hypoxic conditions and escape the cell-cycle arrest. However, their daughters were unable to separate from them and to continue their proliferation under such conditions.
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, 775 15 Olomouc, Czech Republic. rac@tunw.upol.cz
authors.forename: V
authors.initials: V
authors.lastname: Raclavský
authors.forename: J
authors.initials: J
authors.lastname: Pavlícek
authors.forename: R
authors.initials: R
authors.lastname: Novotný
authors.forename: Z
authors.initials: Z
authors.lastname: Moránová
authors.forename: M
authors.initials: M
authors.lastname: Ohkusu
authors.forename: J
authors.initials: J
authors.lastname: Trtková
authors.forename: K
authors.initials: K
authors.lastname: Takeo
authors.forename: S
authors.initials: S
authors.lastname: Kawamoto
mesh_terms: D002455:Cell Division; D003455:Cryptococcus neoformans / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D010100:Oxygen / Q000378:metabolism*
chemical_list: D010100:Oxygen