Analysis of interaction between liposome membranes induced by stress condition: utilization of liposomes immobilized on indium tin oxide electrode.
NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with alpha-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.
Ishii
Haruyuki
H
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Shimanouchi
Toshinori
T
Umakoshi
Hiroshi
H
Kuboi
Ryoichi
R
D000327:Adsorption; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008567:Membranes, Artificial; D018384:Oxidative Stress; D017971:Tin Compounds / Q000737:chemistry*
D008051:Lipid Bilayers; D008081:Liposomes; D008567:Membranes, Artificial; D017971:Tin Compounds; C109984:indium tin oxide