title: Molecular proteomics imaging of tumor interfaces by mass spectrometry.
abstract: The specific molecular profiles of ovarian cancer interface zones (IZ), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Two-dimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interface-specific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDI-IMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors.
authors.affiliation: Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Suki
authors.initials: S
authors.lastname: Kang
authors.forename: Hyo Sup
authors.initials: HS
authors.lastname: Shim
authors.forename: Jong Sik
authors.initials: JS
authors.lastname: Lee
authors.forename: Dong Su
authors.initials: DS
authors.lastname: Kim
authors.forename: Hak Yong
authors.initials: HY
authors.lastname: Kim
authors.forename: Seong Hyun
authors.initials: SH
authors.lastname: Hong
authors.forename: Pan Soo
authors.initials: PS
authors.lastname: Kim
authors.forename: Joo Heon
authors.initials: JH
authors.lastname: Yoon
authors.forename: Nam Hoon
authors.initials: NH
authors.lastname: Cho
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D010051:Ovarian Neoplasms / Q000737:chemistry*; D025341:Principal Component Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*