Allotransplantation of sulphate glucomannan-alginate barium (SGA)-microencapsulated rat islets for the treatment of diabetes mellitus.
To offer a more effective microencapsulation technique of islets for the treatment of diabetes, we have developed a new type of microcapsule comprising sulphate glucomannan-alginate barium (SGA). We compared it with traditional microencapsulated APA (alginate-poly-L-lysine-alginate) and ABa (Ba(2+)-alginate) microencapsulated islets. These three types of microencapsulated islets were prepared and cultured in vitro and we studied their morphology and activity. To determine their effects on insulin secretion and cytokine production (MCP-1, IL-1, IFN-gamma, TNF-alpha) the islets were transplanted into diabetic rats. There was no difference in the morphologies of the three types of microencapsulated islets or their insulin secretory capacity in vitro. However, the SGA microencapsulated islets had higher activity and produced more insulin than the APA and ABa microencapsulated islets after transplantation. Normoglycemia was maintained for longer in the SGA-transplanted group than in the other two groups. The concentrations of cytokines in the peritoneal fluid were significantly decreased in the SGA group, as was the infiltration of inflammatory cells around the microcapsules. In conclusion, the novel SGA microencapsulated islets can maintain normoglycemia in diabetic rats without immunosuppression for longer than APA and ABa microencapsulated islets.
Chen
Xi
X
Dept of Surgery, Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Shao
Wei
W
Chen
Ji-Bing
JB
Zhang
Lei
L
Matthias
Corbascio
C
Shan
Shi-guang
SG
Qi
Zhong-quan
ZQ
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001466:Barium Sulfate / Q000737:chemistry; D001786:Blood Glucose / Q000378:metabolism; D018932:Chemokine CCL2 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery*; D004797:Enzyme-Linked Immunosorbent Assay; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D007515:Islets of Langerhans / Q000737:chemistry / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D008351:Mannans / Q000737:chemistry*; D008863:Microspheres; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000464:Alginates; D001786:Blood Glucose; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D006603:Hexuronic Acids; D007375:Interleukin-1; D008351:Mannans; D014409:Tumor Necrosis Factor-alpha; C051546:alginate-polylysine-alginate; D011107:Polylysine; D001466:Barium Sulfate; C022901:(1-6)-alpha-glucomannan; D007371:Interferon-gamma; D020723:Glucuronic Acid