[Expression of Vitreoscilla hemoglobin gene in Pseudomonas delafieldii R-8 and its application to diesel desulfurization].
OBJECTIVE
To construct an engineering strain of higher desulfurization activity with Vitreoscilla hemoglobin gene (vgb) for desulfurization of diesel.


METHODS
We constructed the vgb expressing plasmid, pPR-Pdsz-vgb and transformed by electroporation to Pseudomonas delafieldii R-8, then obtained a genetic engineering strain R-8-2.


RESULTS
The results of CO-difference spectrum analysis indicated that R-8-2 expressed Vitreoscilla hemoglobin (VHb) having biological activity expressed in strain R-8-2. Compared with the wild-type R-8, the density of strain R-8-2 increased by 20%, and its desulfurization activity is also higher in the lower deliquescence oxygen environment. In the end, about 69.9% of total sulfur in diesel oil was removed by strain R-8-2, whereas only 57.2% of sulfur was removed by strain R-8.


CONCLUSIONS
The activity of strain R-8-2 was enhanced and furthermore, the work is helpful for further development in biodesulfurization.
Li
Huanjie
H
Laboratory of Biochemical and Molecular Biology, Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China. lihuanjie2002@163.com
Yu
Zhijian
Z
Xiong
Xiaochao
X
Li
Yuguang
Y
Wang
Haisheng
H
Li
Xin
X
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D005742:Gasoline / Q000032:analysis*; D015964:Gene Expression Regulation, Bacterial; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D054793:Truncated Hemoglobins / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D005742:Gasoline; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D013455:Sulfur