title: In vitro effects on bacterial growth of phenoloxidase reaction products.
abstract: An active phenoloxidase preparation from the freshwater crayfish Pacifastacus leniusculus exhibited a strong antibacterial effect in vitro on the bacteria Aeromonas hydrophila, Escherichia coli, Streptococcus pneumoniae whereas a weaker but still significant effect against Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus. In most cases reduction of bacterial growth was stronger when dopamine was used as substrate as compared to L-dopa. The effect on bacteria was abolished if no substrate was available for the phenoloxidase or in the presence of the phenoloxidase inhibitor phenylthiourea.
authors.affiliation: Department of Comparative Physiology, Uppsala University, Uppsala, Sweden. Lage.Cerenius@ebc.uu.se
authors.forename: Lage
authors.initials: L
authors.lastname: Cerenius
authors.forename: Ramesh
authors.initials: R
authors.lastname: Babu
authors.forename: Kenneth
authors.initials: K
authors.lastname: Söderhäll
authors.forename: Pikul
authors.initials: P
authors.lastname: Jiravanichpaisal
mesh_terms: D016980:Aeromonas hydrophila / Q000254:growth & development; D000818:Animals; D003400:Astacoidea / Q000201:enzymology*; D001409:Bacillus cereus / Q000254:growth & development; D015169:Colony Count, Microbial; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000254:growth & development; D050296:Microbial Viability / Q000187:drug effects*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010670:Phenylthiourea / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D013211:Staphylococcus aureus / Q000254:growth & development; D013296:Streptococcus pneumoniae / Q000254:growth & development
chemical_list: D004791:Enzyme Inhibitors; D010670:Phenylthiourea; D014442:Monophenol Monooxygenase