High-performance liquid chromatography using UV detection for the simultaneous quantification of ropivacaine and bupivacaine in human plasma.
A specific high-performance liquid chromatography assay coupled with UV detection has been developed and validated for the simultaneous determination of ropivacaine and bupivacaine in human plasma. A liquid-liquid back extraction procedure was used to increase specificity, and very good and consistent recoveries were obtained: 93%-95% for ropivacaine and 90%-96% for bupivacaine. The lowest limit of quantification was 4 and 8 ng/mL for ropivacaine and bupivacaine, respectively. The method was sensitive, reproducible (coefficient of variation <or= 6.9%), and accurate (96%-102%) and was applied successfully to study the pharmacokinetics of ropivacaine and bupivacaine in a perioperative context.
Gaudreault
François
F
Faculté de pharmacie, Université de Montréal, Montréal, Canada.
Drolet
Pierre
P
Varin
France
F
D000577:Amides / Q000097:blood* / Q000493:pharmacokinetics; D053000:Analytic Sample Preparation Methods / Q000379:methods; D000779:Anesthetics, Local / Q000097:blood* / Q000493:pharmacokinetics; D019645:Arthroplasty, Replacement, Knee; D002045:Bupivacaine / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000191:economics / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D000077212:Ropivacaine; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D000577:Amides; D000779:Anesthetics, Local; D000077212:Ropivacaine; D002045:Bupivacaine