NanoPCR observation: different levels of DNA replication fidelity in nanoparticle-enhanced polymerase chain reactions.
Nanoparticle-assisted PCR (polymerase chain reaction) technology is getting more and more attention recently. It is believed that some of the DNA recombinant technologies will be upgraded by nanotechnology in the near future, among which DNA replication is one of the core manipulation techniques. So whether or not the DNA replication fidelity is compromised in nanoparticle-assisted PCR is a question. In this study, a total of 16 different metallic and non-metallic nanoparticles (NPs) were tested for their effects on DNA replication fidelity in vitro and in vivo. Sixteen types of nanomaterials were distinctly different in enhancing the PCR efficiency, and their relative capacity to retain DNA replication fidelity was largely different from each other based on rpsL gene mutation assay. Generally speaking, metallic nanoparticles induced larger error rates in DNA replication fidelity than non-metallic nanoparticles, and non-metallic nanomaterials such as carbon nanopowder or nanotubes were still safe as PCR enhancers because they did not compromise the DNA replication fidelity in the Taq DNA polymerase-based PCR system.
Shen
Cenchao
C
HIT Bio-X Centre for Systems Biotechnology, Harbin Institute of Technology, Weihai 200642, People's Republic of China. scc20001017@gmail.com
Yang
Wenjuan
W
Ji
Qiaoli
Q
Maki
Hisaji
H
Dong
Anjie
A
Zhang
Zhizhou
Z
D004261:DNA Replication / Q000187:drug effects*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000009:adverse effects; D053758:Nanoparticles / Q000009:adverse effects*; D036103:Nanotechnology / Q000379:methods*; D010984:Platinum / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D010984:Platinum; D006046:Gold