title: LC/MS analysis of cellular RNA reveals NAD-linked RNA.
abstract: We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of approximately 3,000 copies per cell.
authors.affiliation: Howard Hughes Medical Institute and the Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Y Grace
authors.initials: YG
authors.lastname: Chen
authors.forename: Walter E
authors.initials: WE
authors.lastname: Kowtoniuk
authors.forename: Isha
authors.initials: I
authors.lastname: Agarwal
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Shen
authors.forename: David R
authors.initials: DR
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D013058:Mass Spectrometry; D009243:NAD / Q000302:isolation & purification* / Q000378:metabolism; D012329:RNA, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D012331:RNA, Fungal / Q000302:isolation & purification* / Q000378:metabolism; D012343:RNA, Transfer / Q000302:isolation & purification* / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D012331:RNA, Fungal; D009243:NAD; D012343:RNA, Transfer