title: Protective effect of Pleurotus cornucopiae mushroom extract on carbon tetrachloride-induced hepatotoxicity.
abstract: Pleurotus cornucopiae (PC) mushrooms are found in the field and commonly known in Japan as Tamogidake mushrooms. The present study investigated the protective effects of an aqueous extract of PC on carbon tetrachloride (CCl4)-induced hepatotoxicity and the possible mechanism involved in this protection including cytochrome P450 (CYP) 2E1. Wistar rats were pretreated with aqueous extracts of PC (0, 100, 200, and 400 mg/kg) orally for 8 days prior to the intraperitoneal administration of a single dose of CCl4 (0.5 ml/kg) or corn oil. Pretreatment with PC mushroom extract significantly prevented the increased serum enzyme activities of alanine and aspartate aminotransferases in a dose-dependent manner, and suppressed the expression of CYP2E1. PC mushroom extract also protected hepatocytes from the damage effects of CCl4 as remarked by histological and electromicroscopical findings. It was concluded that repeated daily doses of aqueous extracts of PC mushroom reduced the toxic effects exerted by CCl4 on the liver.
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, ZagaZig, Egypt.
authors.forename: Bohi Khlood M
authors.initials: BK
authors.lastname: El
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Kaampwe
authors.initials: K
authors.lastname: Muzandu
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ikenaka
authors.forename: Zein Shaban
authors.initials: ZS
authors.lastname: Ibrahim
authors.forename: Akio
authors.initials: A
authors.lastname: Kazusaka
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fujita
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishizuka
mesh_terms: D000363:Agaricales; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics; D017931:DNA Primers; D005784:Gene Amplification; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D010936:Plant Extracts / Q000627:therapeutic use*; D020076:Pleurotus; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D010936:Plant Extracts; D002251:Carbon Tetrachloride; D019392:Cytochrome P-450 CYP2E1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase