Suppression of ongoing experimental allergic encephalomyelitis in DA rats by novel peptide drug, structural part of human myelin basic protein 46-62.
Previously, we demonstrated that autoantibodies (AAb) in multiple sclerosis (MS) reveal site-specific binding and cleavage toward myelin basic protein (MBP) epitope library. We have found several fragments of MBP immunodominant in terms of AAb binding. Here, we applied these peptides to DA rats with induced protracted relapsing experimental allergic encephalomyelitis (EAE) most closely related to MS. DA rats with EAE induced by syngenic spinal cord homogenate in complete Freund's adjuvant were treated by nasal route with human MBP 46-62, 81-102, 124-139, 147-170, and Copaxone. MBP 124-139 and 147-170 displayed only mild therapeutic effects but MBP 46-62 significantly reduced EAE, reflected by lower clinical scores and shorter EAE duration compared to controls.
Belogurov
A A
AA
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya Street, Moscow, 117997, Russia.
Zargarova
T A
TA
Turobov
V I
VI
Novikova
N I
NI
Favorova
O O
OO
Ponomarenko
N A
NA
Gabibov
A G
AG
D000818:Animals; D017151:Antibodies, Catalytic / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000097:blood / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000068717:Glatiramer Acetate; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000627:therapeutic use*; D010455:Peptides / Q000627:therapeutic use; D051381:Rats; D014157:Transcription Factors / Q000276:immunology / Q000627:therapeutic use*
D017151:Antibodies, Catalytic; D001323:Autoantibodies; D001324:Autoantigens; D007166:Immunosuppressive Agents; C499048:MBP protein, human; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D010455:Peptides; D014157:Transcription Factors; D000068717:Glatiramer Acetate