A photolabile linker for the mild and selective cleavage of enriched biomolecules from solid support.
Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.
Orth
Ronald
R
Center for Integrated Protein Science Munich, Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, 81377 Munich, Germany.
Sieber
Stephan A
SA
D002851:Chromatography, High Pressure Liquid; D019906:Nuclear Magnetic Resonance, Biomolecular; D010777:Photochemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays