Functional activation of monoamine transporters by luteolin and apigenin isolated from the fruit of Perilla frutescens (L.) Britt.
Monoamine transporters playing major roles in regulating normal and abnormal synaptic activity are associated with various neuropsychological disorders. In spite of the discovery of a series of structurally different monoamine transporter antagonists for the therapy approach, no practical pharmaceutical can act as a transporter activator. Here, we isolated luteolin and apigenin from the fruit of Perilla frutescens (L.) Britt by using an activity-guided extraction technique, and proved that the two compounds possess actions of enhancing monoamine uptake either upon monoamine-transporter transgenic Chinese hamster ovary (CHO) cells or upon wild dopaminergic cell lines, with higher specificity for dopamine (DA) uptake than for norepinephrine (NE)- and serotonin (5HT)-uptake, as well as with more potency and greater efficacy for luteolin than for apigenin. Further, in the transgenic cells, the principal NE/DA uptake activation by luteolin was significantly prevented by respective transporter inhibitor, and the transmitter-uptake-enhancing action was independent of its ligands, which is in support of the compounds as monoamine transporter activators. Furthermore, luteolin evoked a marked disinhibition of cocaine-targeted effect in CHO cells overexpressing dopamine transporter. Thus, luteolin and apigenin function as monoamine transporter activators, which would improve several hypermonoaminergic neuropsychological disorders, especially cocaine dependence, through up-regulating monoamine transporter activity.
Zhao
Gang
G
Cell Star Bio-Technologies Co., Limited, Shanghai, PR China.
Qin
Guo-Wei
GW
Wang
Jie
J
Chu
Wen-Jing
WJ
Guo
Li-He
LH
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology*; D016466:CHO Cells; D002460:Cell Line; D003042:Cocaine / Q000819:agonists; D019970:Cocaine-Related Disorders / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D006224:Cricetinae; D003412:Cricetulus; D004298:Dopamine / Q000378:metabolism; D018765:Dopamine Uptake Inhibitors / Q000819:agonists; D047311:Luteolin / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology; D050493:Vesicular Monoamine Transport Proteins / Q000187:drug effects* / Q000378:metabolism
D000975:Antioxidants; D018765:Dopamine Uptake Inhibitors; D010936:Plant Extracts; D050493:Vesicular Monoamine Transport Proteins; D012701:Serotonin; D047310:Apigenin; D003042:Cocaine; D047311:Luteolin; D004298:Dopamine; D009638:Norepinephrine