title: Identification of functional marker proteins in the mammalian growth cone.
abstract: Identification of proteins in the mammalian growth cone has the potential to advance our understanding of this critical regulator of neuronal growth and formation of neural circuit; however, to date, only one growth cone marker protein, GAP-43, has been reported. Here, we successfully used a proteomic approach to identify 945 proteins present in developing rat forebrain growth cones, including highly abundant, membrane-associated and actin-associated proteins. Almost 100 of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 17 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus their identification presents a significant step forward, providing marker proteins and candidate neuronal growth-associated proteins.
authors.affiliation: Divisions of Molecular Cell Biology and Ophthalmology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Nozumi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Togano
authors.forename: Kazuko
authors.initials: K
authors.lastname: Takahashi-Niki
authors.forename: Jia
authors.initials: J
authors.lastname: Lu
authors.forename: Atsuko
authors.initials: A
authors.lastname: Honda
authors.forename: Masato
authors.initials: M
authors.lastname: Taoka
authors.forename: Takashi
authors.initials: T
authors.lastname: Shinkawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koga
authors.forename: Kosei
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Isobe
authors.forename: Michihiro
authors.initials: M
authors.lastname: Igarashi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000032:analysis*; D051177:Catenins / Q000032:analysis / Q000235:genetics; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics; D009414:Nerve Growth Factors / Q000032:analysis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D016548:Prosencephalon / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D034622:RNA Interference; D051381:Rats; D055647:Rats, Transgenic; D013997:Time Factors
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D051177:Catenins; C413755:Dpysl5 protein, rat; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020543:Proteome; C085284:microtubule-associated protein 1B; D049452:Green Fluorescent Proteins