title: Developmental regulation of the composite CAG promoter activity in the murine T lymphocyte cell lineage.
abstract: Promoter selection is of utmost importance for the study of in vivo gene function using transgenic models. In the present study, we have analyzed the expression of the GFP marker under the control of the composite CAG promoter in the lymphoid compartment of several transgenic mouse strains. Despite the ability of the CAG promoter to drive gene expression in almost all tissues examined to date, its activity appears to be developmentally regulated within the T lymphocyte cell lineage. In particular, CD4 and CD8-expressing, thymic immature T cells displayed lower levels of the GFP marker when compared with both bone marrow precursors and mature circulating T cells, suggesting a transient downregulation of CAG activity during T cell development. Alternative promoters may therefore be preferred for the study of T cell development in vivo using a transgenic approach.
authors.forename: Delphine
authors.initials: D
authors.lastname: Baup
authors.forename: Muriel
authors.initials: M
authors.lastname: Moser
authors.forename: Stéphane
authors.initials: S
authors.lastname: Schurmans
authors.forename: Oberdan
authors.initials: O
authors.lastname: Leo
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002460:Cell Line; D019070:Cell Lineage; D002645:Chickens; D003587:Cytomegalovirus / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D019076:Transgenes / Q000235:genetics
chemical_list: D000199:Actins; D018941:Antigens, CD19; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D049452:Green Fluorescent Proteins