Reverse breeding: a novel breeding approach based on engineered meiosis.
Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on reducing genetic recombination in the selected heterozygote by eliminating meiotic crossing over. Male or female spores obtained from such plants contain combinations of non-recombinant parental chromosomes which can be cultured in vitro to generate homozygous doubled haploid plants (DHs). From these DHs, complementary parents can be selected and used to reconstitute the heterozygote in perpetuity. Since the fixation of unknown heterozygous genotypes is impossible in traditional plant breeding, RB could fundamentally change future plant breeding. In this review, we discuss various other applications of RB, including breeding per chromosome.
Dirks
Rob
R
Rijk Zwaan Breeding BV, Fijnaart, The Netherlands.
van Dun
Kees
K
de Snoo
C Bastiaan
CB
van den Berg
Mark
M
Lelivelt
Cilia L C
CL
Voermans
William
W
Woudenberg
Leo
L
de Wit
Jack P C
JP
Reinink
Kees
K
Schut
Johan W
JW
van der Zeeuw
Eveline
E
Vogelaar
Aat
A
Freymark
Gerald
G
Gutteling
Evert W
EW
Keppel
Marina N
MN
van Drongelen
Paul
P
Kieny
Matthieu
M
Ellul
Philippe
P
Touraev
Alisher
A
Ma
Hong
H
de Jong
Hans
H
Wijnker
Erik
E
D001947:Breeding / Q000379:methods*; D018556:Crops, Agricultural / Q000235:genetics / Q000254:growth & development; D003434:Crossing Over, Genetic; D055785:Gene Knockdown Techniques; D018745:Genome, Plant; D006579:Heterozygote; D008540:Meiosis; D063245:Plant Development; D010944:Plants / Q000235:genetics