title: [Effect of biological pretreatment with Trametes vesicolor on the enzymatic hydrolysis of softwood and hardwood].
abstract: We evaluated the effect of biological pretreatment with white rot fungus Trametes vesicolor on the enzymatic hydrolysis of two wood species, Chinese willow (Salix babylonica, hardwood) and China-fir (Cunninghamia lanceolata, softwood). The result indicated that the pretreated woods showed significant increases in the final conversion ratios of enzymatic hydrolysis (4.78-fold for hardwood and 4.02-fold for softwood). In order to understand the role of biological pretreatment we investigated the enzyme-substrate interactions. Biological pretreatment enhanced the substrate accessibility to cellulase but not always correlated with the initial conversion rate. However, the change of the conversion rate decreased dramatically with increased desorption values after biological pretreatment. Thus, the biological pretreatment slowed down the declines in conversion rates during enzymatic hydrolysis by reducing the irreversible adsorption of cellulase and then improved the enzymatic hydrolysis. Moreover, the decreases of the irreversible adsorption may be attributed to the partial lignin degradation and alteration in lignin structure after biological pretreatment.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. hongbo.fish@gmail.com
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D002480:Cellulase / Q000378:metabolism*; D032486:Cunninghamia / Q000378:metabolism / Q000382:microbiology; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D032108:Salix / Q000378:metabolism / Q000382:microbiology; D055454:Trametes / Q000378:metabolism / Q000502:physiology*; D014934:Wood / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D008031:Lignin; D002480:Cellulase