title: Enzymatic production of linalool esters in organic and solvent-free system.
abstract: This work investigated the influence of temperature, enzyme concentration, substrates molar ratio, in the absence and presence of organic solvent, at two molar ratios of the substrates on the enzymatic production of linalil esters using the immobilized lipase Novozym 435 as catalyst, different acids and linalool and Ho-Sho essential oil as substrates. The best reaction conversion was obtained at the highest temperature (70 degrees C), for both solvent free (3.81%) and with solvent addition (2.25%), for a solvent to substrates molar ratio of 2:1, enzyme concentration of 5 wt% and acid to alcohol molar ratio of 1:1. The reaction kinetics revealed that Ho-Sho essential oil afforded the greatest conversions when compared with pure linalool. Higher linalil esters production were achieved after 10 h reaction (5.58%) in 2:1 solvent to substrates molar ratio, with enzyme concentration of 5 wt%, at 70 degrees C and anhydride to alcohol molar ratio of 1:1 using Ho-Sho essential oil as substrate.
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil.
authors.forename: Natália
authors.initials: N
authors.lastname: Paroul
authors.forename: Adriana
authors.initials: A
authors.lastname: Biasi
authors.forename: Ane Cheila
authors.initials: AC
authors.lastname: Rovani
authors.forename: Camile
authors.initials: C
authors.lastname: Prigol
authors.forename: Rogerio
authors.initials: R
authors.lastname: Dallago
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: Rogério L
authors.initials: RL
authors.lastname: Cansian
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D000080462:Acyclic Monoterpenes; D002175:Candida / Q000201:enzymology*; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D039821:Monoterpenes / Q000138:chemical synthesis* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
chemical_list: D000080462:Acyclic Monoterpenes; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D039821:Monoterpenes; D012997:Solvents; C018584:linalool; C093667:Novozyme 435; D008049:Lipase