Characterization of Bacillus polyfermenticus KJS-2 as a probiotic.
The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be 60.71+/-4.9%. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of 140+/-7.9% and 108+/-3.5%, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately 65+/-0.6%.
Kim
Kang Min
KM
Department of Smart Foods and Drugs, Inje University, Gyeongsangnam-do 621-749, Korea.
Kim
Myo Jeong
MJ
Kim
Dong Hee
DH
Park
You Soo
YS
Kang
Jae Seon
JS
D001407:Bacillus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D001422:Bacterial Adhesion; D001647:Bile Acids and Salts / Q000494:pharmacology; D018938:Caco-2 Cells / Q000382:microbiology; D005285:Fermentation; D005750:Gastric Juice; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009005:Monosaccharides / Q000378:metabolism; D019936:Probiotics / Q000737:chemistry*; D013171:Spores, Bacterial / Q000502:physiology / Q000648:ultrastructure; D013379:Substrate Specificity
D001647:Bile Acids and Salts; D009005:Monosaccharides