title: Amino acid substitutions and intragenic duplications of Bacillus sp. PS3 flagellin cause complementation of the Bacillus subtilis flagellin deletion mutant.
abstract: Bacillus sp. PS3 produces a glycosylated flagellin. In this study, a number of the glycosylated residues of the flagellin protein were found to be located in the central variable region of this protein. We also report that the motility defect of the Bacillus subtilis flagellin mutant was complemented by Bacillus sp. PS3 flagellin variants without glycosylation, which contained amino acid substitutions and intragenic duplications in the variable region of flagellin.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Tokyo, Japan.
authors.forename: Jumpei
authors.initials: J
authors.lastname: Hayakawa
authors.forename: Takao
authors.initials: T
authors.lastname: Kambe
authors.forename: Morio
authors.initials: M
authors.lastname: Ishizuka
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001412:Bacillus subtilis / Q000235:genetics*; D005408:Flagellin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020440:Gene Duplication; D005816:Genetic Complementation Test; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017384:Sequence Deletion
chemical_list: D005408:Flagellin