title: Qualea parviflora Mart.: an integrative study to validate the gastroprotective, antidiarrheal, antihemorragic and mutagenic action.
abstract: BACKGROUND
  Qualea parviflora Mart. is a medicinal species commonly found in the Brazilian Cerrado biome.
  
  
  OBJECTIVE
  Based on ethnopharmacological data, methanolic extract from Qualea parviflora (QP) bark was evaluated for its antiulcer, analgesic, anti-hemorrhagic, mutagenic and anti-Helicobacter pylori activities.
  
  
  METHODS
  The gastroprotective action of the extract was evaluated in rodent experimental models (HCl/ethanol, ethanol or NSAID). We also evaluated mutagenic effect (Ames assay), anti-Helicobacter pylori, anti-hemorrhagic action, analgesic and inflammatory effects (hot-plate test and carrageenin-induced hind paw edema) of methanolic extract from Qualea parviflora.
  
  
  RESULTS
  QP (500 mg/kg, p.o.) was able to protect gastric mucosa against HCl/ethanol solution (77%), absolute ethanol (97%), and also against injurious effect of NSAID (36%). When QP was challenged with sulfhydryl depletor compound, the gastroprotective action of extract was abolished. QP treatment was able to maintain the GSH level and show a concentration-dependent inhibition effect on the lipid peroxidation. QP present anti-Helicobacter pylori effect (MIC=75 microg/mL), anti-hemorrhagic and antidiarrheal action but not present analgesic or anti-inflammatory effect.
  
  
  CONCLUSIONS
  methanolic extract from Qualea parviflora had gastroprotective effect related to the increase of gastric mucosa defensive factors such PGE(2) levels and maintain the basal gastric glutathione levels. The methanolic extract also showed anti-Helicobacter pylori activity, anti-hemorrhagic effect and antioxidant action, but absence of analgesic, mutagenic and toxic effects, a profile that adds safety to its use.
authors.affiliation: Physiology Department, Biosciences Institute, São Paulo State University-UNESP, cp 510, Zip Code 18618-000 Botucatu, SP, Brazil.
authors.forename: Lucilene Patrícia
authors.initials: LP
authors.lastname: Mazzolin
authors.forename: Ana Lúcia Martiniano
authors.initials: AL
authors.lastname: Nasser
authors.forename: Thiago Mello
authors.initials: TM
authors.lastname: Moraes
authors.forename: Raquel Cássia
authors.initials: RC
authors.lastname: Santos
authors.forename: Catarine Massucato
authors.initials: CM
authors.lastname: Nishijima
authors.forename: Fábio Vieira
authors.initials: FV
authors.lastname: Santos
authors.forename: Eliana Aparecida
authors.initials: EA
authors.lastname: Varanda
authors.forename: Taís Maria
authors.initials: TM
authors.lastname: Bauab
authors.forename: Lúcia Regina Machado
authors.initials: LR
authors.lastname: da Rocha
authors.forename: Luis Cláudio
authors.initials: LC
authors.lastname: Di Stasi
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.forename: Clélia Akiko
authors.initials: CA
authors.lastname: Hiruma-Lima
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000930:Antidiarrheals / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D016480:Helicobacter pylori / Q000187:drug effects / Q000502:physiology; D006490:Hemostatics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000897:Anti-Ulcer Agents; D000930:Antidiarrheals; D006490:Hemostatics; D010936:Plant Extracts