A comparison of two microbial detection methods used in aseptic processing of musculoskeletal allograft tissues.
Tissues from 78 musculoskeletal donors were concurrently tested for microorganisms using both a swab and liquid culture method. An aggregate total of 20 organisms were detected by both methods. The swab detected 4/20 organisms while the liquid culture detected 18/20 organisms. The swab method yielded sensitivity and negative predictive values of 20 and 92.3%, respectively. Comparatively, the liquid culture displayed a sensitivity of 90% and a negative predictive value of 99%. These results clearly demonstrate that the liquid culture method is superior to swab cultures in microbial detection. Additional studies are necessary to determine the optimal culture conditions for different types of tissues when utilizing the liquid culture method.
Dennis
Jeremy A
JA
University of Miami Tissue Bank, Department of Orthopaedics, University of Miami Miller School of Medicine, 1600 NW 10th Avenue, Miami, FL 33136, USA.
Martinez
Octavio V
OV
Landy
David C
DC
Malinin
Theodore I
TI
Morris
Paul R
PR
Fox
William P
WP
Buck
Billy E
BE
Temple
H Thomas
HT
D001419:Bacteria / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods*; D016025:Bone Transplantation; D001842:Bone and Bones / Q000382:microbiology*; D006801:Humans; D011237:Predictive Value of Tests; D046509:Tissue Culture Techniques; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014184:Transplantation, Homologous