title: T lineage differentiation from induced pluripotent stem cells.
abstract: Induced pluripotent stem (iPS) cells have potential to differentiate into T lymphocytes, however, the actual ability of iPS cells to develop into T lineages is not clear. In this study, we co-cultured iPS cells on OP9 cells expressing the Notch ligand Delta-like 1 (DL1), the iPS cells differentiated into T lymphocytes. In addition, in vitro stimulation of iPS cell-derived T lymphocytes resulted in secretion of IL-2 and IFN-gamma. Moreover, adoptive transfer of iPS cell-derived T lymphocytes into Rag-deficient mice reconstituted their T cell pools. These results indicate that iPS cells are able to follow the normal program of T cell differentiation.
authors.affiliation: Department of Microbiology & Immunology and Penn State Hershey Cancer Institute, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
authors.forename: Fengyang
authors.initials: F
authors.lastname: Lei
authors.forename: Rizwanul
authors.initials: R
authors.lastname: Haque
authors.forename: Lauren
authors.initials: L
authors.lastname: Weiler
authors.forename: Kent E
authors.initials: KE
authors.lastname: Vrana
authors.forename: Jianxun
authors.initials: J
authors.lastname: Song
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D019070:Cell Lineage / Q000276:immunology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000276:immunology; D051880:Receptors, Notch / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D051880:Receptors, Notch