Estimation of antioxidant capacity against pathophysiologically relevant oxidants using Pyrogallol Red.
Peroxynitrite and hypochlorite are oxidants relevant in many pathological situations. We propose a simple spectrophotometric assay to determine antioxidant capacity against hypochlorite and peroxynitrite based on protection against Pyrogallol Red decolorization. The assay can be performed on a microplate and requires minute amounts of material. Standard antioxidants show different reactivities for both oxidants. Antioxidant capacity of blood plasma (anticoagulated with EDTA) of healthy persons was found to be 559+/-49 micromol/l and 11.6+/-1.2 mmol/l of ascorbic acid equivalents for peroxynitrite and hypochlorite, respectively.
Robaszkiewicz
Agnieszka
A
Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. agnieszka.robaszkiewicz@gmail.com
Bartosz
Grzegorz
G
D000975:Antioxidants / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry*; D006801:Humans; D006997:Hypochlorous Acid / Q000037:antagonists & inhibitors*; D016877:Oxidants / Q000037:antagonists & inhibitors*; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors*; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
D000975:Antioxidants; D016877:Oxidants; D011748:Pyrogallol; D030421:Peroxynitrous Acid; C058731:pyrogallol sulfonphthalein; D006997:Hypochlorous Acid