Periodontal gene transfer by ultrasound and nano/microbubbles.
A non-viral gene delivery approach with nano/microbubbles and ultrasound offers opportunities for targeting soft tissues for gene therapy. The periodontium is a complex structure comprised of hard (cementum, alveolar bone) and soft tissues (periodontal ligament, gingivae). We hypothesized that our established gene delivery method would allow the periodontal tissue to be targeted for transfection for gene therapy. Expression kinetics and sites of transfection sites with this approach were investigated in rat periodontal tissue. Bioluminescence imaging revealed that transient gene expression was induced at day 1 posttransfection, while confocal microscopy showed that gene expression was localized in the muscle cells of gingival tissues. These findings indicate that regular transfection with this approach results in high gene expression, facilitating gene therapy for periodontal disease involving alveolar bone resorption.
Chen
R
R
Molecular Delivery System Laboratory, Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
Chiba
M
M
Mori
S
S
Fukumoto
M
M
Kodama
T
T
D000818:Animals; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques / Q000295:instrumentation; D017930:Genes, Reporter; D015316:Genetic Therapy; D005822:Genetic Vectors; D005881:Gingiva / Q000378:metabolism; D049452:Green Fluorescent Proteins; D049409:Luciferases, Firefly; D049449:Luminescence; D049408:Luminescent Agents; D008297:Male; D045423:Microbubbles; D018613:Microscopy, Confocal; D032342:Muscle Cells / Q000378:metabolism; D049329:Nanostructures; D010510:Periodontal Diseases / Q000628:therapy; D010519:Periodontium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013539:Simian virus 40 / Q000235:genetics; D013997:Time Factors; D014162:Transfection; D014465:Ultrasonics
D005456:Fluorescent Dyes; D049408:Luminescent Agents; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D049409:Luciferases, Firefly