[Effect of spinal glutamate transporter 1 on chronic constriction injury of sciatic nerve and morphine tolerance of rats].
In order to investigate the role of spinal glutamate transporter 1 (GLT-1) in the neuropathic pain and morphine tolerance, rat chronic constriction injury (CCI) of sciatic nerve was performed, and the mechanical allodynia was evaluated by mechanical withdrawal threshold (MWT), the expression of GLT-1 was measured by real-time PCR and Western blotting analysis. The results showed that compared to sham group, the MWT of CCI group had decreased approximately 80%. Administration of morphine alone could develop tolerance rapidly in initial two days, and then had no significant difference with CCI group, the expression of GLT-1 was down-regulated. Ceftriaxone sodium alone could improve mechanical allodynia. Co-administration of ceftriaxone sodium with morphine attenuated morphine tolerance and up-regulated GLT-1 expression, and the MWT remained at high level after 6 days. In conclusion, change of spinal GLT-1 expression and function has close correlation with the development of neuropathic pain and morphine tolerance.
Yan
Hui
H
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Li
Cheng-Min
CM
Li
Yu-Lei
YL
Gong
Ze-Hui
ZH
D000818:Animals; D004361:Drug Tolerance; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D011843:Radiculopathy / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000473:pathology*; D020426:Sciatic Neuropathy / Q000378:metabolism* / Q000473:pathology; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism
D027342:Excitatory Amino Acid Transporter 2; C493395:Slc1a2 protein, rat; D009020:Morphine