title: Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the resuscitation promoting factor interacting protein RipA from M. tuberculosis.
abstract: RipA is an important growth factor of M. tuberculosis. Its depletion produces decreasing bacterial growth and an abnormal phenotype. RipA C-terminal fragment (263-472), containing its predicted catalytic domain has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and atomic resolution refinement is in progress.
authors.affiliation: Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16. I-80134 Napoli, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Ruggiero
authors.forename: Flavia
authors.initials: F
authors.lastname: Squeglia
authors.forename: Carla
authors.initials: C
authors.lastname: Esposito
authors.forename: Daniela
authors.initials: D
authors.lastname: Marasco
authors.forename: Emilia
authors.initials: E
authors.lastname: Pedone
authors.forename: Carlo
authors.initials: C
authors.lastname: Pedone
authors.forename: Rita
authors.initials: R
authors.lastname: Berisio
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D016207:Cytokines / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D011994:Recombinant Proteins; C113704:resuscitation-promoting factor, bacteria