Regulation of ciliary polarity by the APC/C.
Planar cell polarity signaling controls a variety of polarized cell behaviors. In multiciliated Xenopus epidermal cells, recruitment of Dishevelled (Dvl) to the basal body and its localization to the center of the ciliary rootlet are required to correctly position the motile cilia. We now report that the anaphase-promoting complex (APC/C) recognizes a D-box motif of Dvl and ubiquitylates Dvl on a highly conserved lysine residue. Inhibition of APC/C function by knockdown of the ANAPC2 subunit disrupts the polarity of motile cilia and alters the directionality of the fluid movement along the epidermis of the Xenopus embryo. Our results suggest that the APC/C activity enables cilia to correctly polarize in Xenopus epidermal cells.
Ganner
Athina
A
Renal Division, Department of Medicine, University Hospital Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany.
Lienkamp
Soeren
S
Schäfer
Tobias
T
Romaker
Daniel
D
Wegierski
Tomasz
T
Park
Tae Joo
TJ
Spreitzer
Stefan
S
Simons
Matias
M
Gloy
Joachim
J
Kim
Emily
E
Wallingford
John B
JB
Walz
Gerd
G
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D030801:Animals, Genetically Modified; D064190:Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome; D001483:Base Sequence; D002460:Cell Line; D016764:Cell Polarity / Q000502:physiology*; D002923:Cilia / Q000502:physiology*; D017124:Conserved Sequence; D000072261:Dishevelled Proteins; D000078404:Epidermal Cells; D005260:Female; D018390:Gene Targeting; D006801:Humans; D051379:Mice; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D043743:Ubiquitin-Protein Ligase Complexes / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051153:Wnt Proteins / Q000378:metabolism; D029867:Xenopus Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics / Q000502:physiology
D048868:Adaptor Proteins, Signal Transducing; C000606586:DVL1 protein, Xenopus; D000072261:Dishevelled Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D010750:Phosphoproteins; D011994:Recombinant Proteins; D051153:Wnt Proteins; D029867:Xenopus Proteins; D043743:Ubiquitin-Protein Ligase Complexes; C577341:Anapc2 protein, mouse; D064173:Anaphase-Promoting Complex-Cyclosome; D064190:Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome