title: Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels.
abstract: Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived strain A/J, and the Mus musculus castaneus derived strain CAST/Ei. We describe approaches for the accurate identification of nucleotide and structural variation in the genomes of vertebrate experimental organisms, and show how these techniques can be applied to help prioritize candidate genes within quantitative trait loci.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1HH, UK. ims@sanger.ac.uk
authors.forename: Ian
authors.initials: I
authors.lastname: Sudbery
authors.forename: Jim
authors.initials: J
authors.lastname: Stalker
authors.forename: Jared T
authors.initials: JT
authors.lastname: Simpson
authors.forename: Thomas
authors.initials: T
authors.lastname: Keane
authors.forename: Alistair G
authors.initials: AG
authors.lastname: Rust
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Hurles
authors.forename: Klaudia
authors.initials: K
authors.lastname: Walter
authors.forename: Dee
authors.initials: D
authors.lastname: Lynch
authors.forename: Lydia
authors.initials: L
authors.lastname: Teboul
authors.forename: Steve D
authors.initials: SD
authors.lastname: Brown
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Zemin
authors.initials: Z
authors.lastname: Ning
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Nadeau
authors.forename: Colleen M
authors.initials: CM
authors.lastname: Croniger
authors.forename: Richard
authors.initials: R
authors.lastname: Durbin
authors.forename: David J
authors.initials: DJ
authors.lastname: Adams
mesh_terms: D000818:Animals; D001483:Base Sequence; D033481:Chromosomes, Mammalian / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics; D018628:Gene Dosage / Q000235:genetics; D016678:Genome / Q000235:genetics; D018095:Germ-Line Mutation / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008285:Major Histocompatibility Complex / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011786:Quality Control; D040641:Quantitative Trait Loci / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D017422:Sequence Analysis, DNA / Q000379:methods*; D017384:Sequence Deletion / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D018388:Codon, Terminator; D012333:RNA, Messenger; D014280:Triglycerides