title: Osterix enhances BMSC-associated osseointegration of implants.
abstract: Cellular and molecular events in osseointegration at the dental implant surface remain largely unknown. We hypothesized that bone marrow stromal cells (BMSCs) participate in this process, and that osterix (Osx) promotes implant osseointegration. To prove this hypothesis, we tracked double-labeled BMSCs in implantation sites created in nude mice transplanted with these cells. We also inserted implants into the femurs of our established transgenic mice after local administration of viruses encoding Osx, to determine the osteogenic effects of Osx. Immunohistochemical results demonstrated that BMSCs can recruit from peripheral circulation and participate in wound healing and osseointegration after implantation. Microcomputed tomography (microCT) analysis revealed an increased bone density at the bone-to-implant interface in the Osx group, and histomorphometric analysis indicated an elevated level of bone-to-implant contact in the Osx group. We concluded that exogenous BMSCs participate in the osseointegration after implantation, and that Osx overexpression accelerates osseointegration.
authors.affiliation: Division of Oral Biology, Tufts University School of Dental Medicine, One Kneeland Street, Boston, MA 02111, USA.
authors.forename: B
authors.initials: B
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: E
authors.initials: E
authors.lastname: Brewer
authors.forename: Q
authors.initials: Q
authors.lastname: Tu
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Tang
authors.forename: P
authors.initials: P
authors.lastname: Krebsbach
authors.forename: M
authors.initials: M
authors.lastname: Wieland
authors.forename: J
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D015921:Dental Implants; D005269:Femur / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D058574:Integrin-Binding Sialoprotein; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D016348:Osseointegration / Q000187:drug effects* / Q000502:physiology; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D012795:Sialoglycoproteins / Q000032:analysis; D000076182:Sp7 Transcription Factor; D017154:Stromal Cells / Q000187:drug effects* / Q000502:physiology; D014157:Transcription Factors / Q000032:analysis / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology; D055114:X-Ray Microtomography; D016335:Zinc Fingers / Q000502:physiology*
chemical_list: D015921:Dental Implants; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D012795:Sialoglycoproteins; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors; D015675:Osteocalcin