The effect of DNA aptamer configuration on the sensitivity of detection thrombin at surface by acoustic method.
Using an acoustic method we showed that the aptamer configuration, especially those comprised of dimers with binding exosites sensitive to heparin and fibrinogen at thrombin, substantially increases the aptasensor sensitivity.
Hianik
Tibor
T
Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina F1, 84248 Bratislava, Slovakia. tibor.hianik@fmph.uniba.sk
Grman
Igor
I
Karpisova
Ivana
I
D000162:Acoustics; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
D052157:Aptamers, Nucleotide; D013917:Thrombin