Distribution of HLA-DRB1 alleles in Argentinean patients with Chagas' disease cardiomyopathy.
Chronic Chagas' disease occurs in a variable number of infected individuals and mainly manifests as an inflammatory cardiomyopathy that may lead to a fatal course. The factors underlying the establishment of chronic myocardial lesions are not fully understood. The study included 71 unrelated individuals serologically positive for T. cruzi. A group of 81 no related healthy individuals with neither symptoms nor previous diagnosis of Chagas' disease was studied as control group. Genomic DNA was extracted from peripheral blood using the standard salting out method and used as a template to amplify by the PCR the polymorphic second exon of the HLA-DRB1. PCR products were hybridized separately with sequence-specifics oligonucleotides (SSOP). DRB1*0409 and DRB1*1503 alleles were significantly more prevalent in seropositives (pC = 0.002, OR: 26.17 and 24.87 respectively). The prevalence of DRB1*1103 allele was statistically significant in the group control and could be associated with resistance Chagas' disease (pC = 0.026, OR: 0.19). Increased significance frequency of DRB1*1503 allele was found among cardiomyopathy patients suggesting that this antigen might be related with the genetic susceptibility to cardiac damage in these patients (pC = 0.014, OR: 9.22).
García Borrás
Silvia
S
Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina. sigarcia@fbioyf.unr.edu.ar
Racca
Liliana
L
Cotorruelo
Carlos
C
Biondi
Claudia
C
Beloscar
Juan
J
Racca
Amelia
A
D000483:Alleles; D001118:Argentina; D002598:Chagas Cardiomyopathy / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains