Zinc-dependent binding between peptides derived from rainbow trout CD8alpha and LCK.
The cytoplasmic tail of mammalian CD8alpha binds the kinase LCK in a zinc-dependent manner. In analogy with a previous study for humans (Kim et al., 2003) peptides were synthesized from rainbow trout CD8alpha and LCK. Surface plasmon resonance (SPR) analysis indicated that also in fish these molecules bind to each other in a zinc-dependent manner.
Hayashi
Nobuhiro
N
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Takeuchi
Masami
M
Nakanishi
Teruyuki
T
Hashimoto
Keiichiro
K
Dijkstra
Johannes Martinus
JM
D000818:Animals; D001665:Binding Sites; D016827:CD8 Antigens / Q000378:metabolism*; D002645:Chickens; D006801:Humans; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism*; D051379:Mice; D017686:Oncorhynchus mykiss / Q000276:immunology / Q000378:metabolism*; D016415:Sequence Alignment; D020349:Surface Plasmon Resonance; D015032:Zinc / Q000378:metabolism / Q000502:physiology*
D016827:CD8 Antigens; C431698:CD8alpha antigen; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D015032:Zinc