Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells.
BACKGROUND
Adipogenesis is a complex process that involves many genes/proteins at different stages of differentiation. In order to identify genes critical for adipogenesis, we took a novel approach based on phenotype change of individual cell, to search for genes with regulatory roles in adipogenesis genome-wide in 3T3-L1 cells.


METHODS
Lentivirus-based inducible random homologous knockdown was used for the screening of functional gene that altered lipid formation in the adipocyte during differentiation.


RESULTS
In the present study, we reported the identification of an alternatively spliced mitochondrial oxodicarboxylate carrier (ODC), so named ODC-AS. ODC-AS is different from ODC by replacing 22 amino acids with 29 amino acids at the N-terminal. ODC was widely expressed in most tissues in mouse as determined by multi-tissue cDNA panel polymerase chain reaction. However, ODC-AS was only detected in adipose tissue and in iris and sclera-choroid complex of the eye. The expression of ODC-AS in 3T3-L1 was detected after the induction of differentiation, and reached a peak at day 4 and then reduced thereafter, whereas no ODC transcript detected in the cells neither before nor after differentiation. Knocking down of ODC-AS expression by RNA interference led to significant reduction in lipid accumulation as determined by triglyceride measurement and Nile Red staining, as well as adipogenic marker CEBPalpha, PPARgamma, aP2 and CD36. Although both ODC and ODC-AS are expressed in white and brown adipose tissues, only the expression of ODC-AS was down-regulated in brown adipose tissue by cold exposure.


CONCLUSIONS
These results implicate that ODC-AS may promote lipid accumulation during adipocyte differentiation and play an important role in the regulation of lipid metabolism in adipose tissues.
Niimi
Masashi
M
Otsuka Maryland Medicinal Laboratories, Inc, Rockville, Maryland, USA.
Tao
Lian
L
Lin
Shi-Hua
SH
Yin
Jun
J
Wu
Xiaoyun
X
Fukui
Hiroyuki
H
Kambayashi
Junichi
J
Ye
Jianping
J
Sun
Bing
B
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D002264:Carboxylic Acids / Q000378:metabolism*; D002454:Cell Differentiation; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D016678:Genome; D016086:Lentivirus / Q000235:genetics; D008055:Lipids / Q000737:chemistry; D051379:Mice; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics / Q000502:physiology*; D009928:Organ Specificity; D027361:Organic Anion Transporters / Q000235:genetics / Q000502:physiology*; D010641:Phenotype
D002264:Carboxylic Acids; D008055:Lipids; D033681:Mitochondrial Membrane Transport Proteins; D027361:Organic Anion Transporters