Native electrophoretic techniques to identify protein-protein interactions.
Permanent protein-protein interactions are commonly identified by co-purification of two or more protein components using techniques like co-immunoprecipitation, tandem affinity purification and native electrophoresis. Here we focus on blue-native electrophoresis, clear-native electrophoresis, high-resolution clear-native electrophoresis and associated techniques to identify stable membrane protein complexes and detergent-labile physiological supercomplexes. Hints for dynamic protein-protein interactions can be obtained using two-hybrid techniques but not from native electrophoresis and other protein isolation techniques except after covalent cross-linking of interacting proteins in vivo prior to protein separation.
Wittig
Ilka
I
Cluster of Excellence Macromolecular Complexes, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Goethe-Universität Frankfurt, Frankfurt am Main, Germany.
Schägger
Hermann
H
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods* / Q000639:trends; D006801:Humans; D025941:Protein Interaction Mapping / Q000379:methods* / Q000639:trends; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
D011506:Proteins