title: Influence of thermal processing on IgE reactivity to lentil and chickpea proteins.
abstract: In the last years, legume proteins are gaining importance as food ingredients because of their nutraceutical properties. However, legumes are also considered relevant in the development of food allergies through ingestion. Peanuts and soybeans are important food allergens in Western countries, while lentil and chickpea allergy are more relevant in the Mediterranean area. Information about the effects of thermal-processing procedures at various temperatures and conditions is scarce; therefore, the effect of these procedures on legume allergenic properties is not defined so far. The SDS-PAGE and IgE-immunoblotting patterns of chickpeas and lentils were analyzed before and after boiling (up to 60 min) and autoclaving (1.2 and 2.6 atm, up to 30 min). The results indicated that some of these treatments reduce IgE binding to lentil and chickpea, the most important being harsh autoclaving. However, several extremely resistant immunoreactive proteins still remained in these legumes even after this extreme treatment.
authors.affiliation: Departamento de Tecnología de Alimentos, SGIT-INIA, Madrid, Spain. cuadrado@inia.es
authors.forename: Carmen
authors.initials: C
authors.lastname: Cuadrado
authors.forename: Beatriz
authors.initials: B
authors.lastname: Cabanillas
authors.forename: Mercedes M
authors.initials: MM
authors.lastname: Pedrosa
authors.forename: Alejandro
authors.initials: A
authors.lastname: Varela
authors.forename: Eva
authors.initials: E
authors.lastname: Guillamón
authors.forename: Mercedes
authors.initials: M
authors.lastname: Muzquiz
authors.forename: Jesús F
authors.initials: JF
authors.lastname: Crespo
authors.forename: Julia
authors.initials: J
authors.lastname: Rodriguez
authors.forename: Carmen
authors.initials: C
authors.lastname: Burbano
mesh_terms: D000485:Allergens / Q000276:immunology*; D029863:Cicer / Q000276:immunology*; D005511:Food Handling; D005512:Food Hypersensitivity / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D027806:Lens Plant / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*
chemical_list: D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E