title: Rapid data analysis method for differential mobility cytometry.
abstract: Differential mobility cytometry (DMC) has recently been established as a powerful method to capture cells and study adhesion processes. DMC uses an oscillation system and cell affinity chromatography to monitor cells as they adhere to a surface. In the past, differential images had to be created individually which limited the throughput of the method. A new method to create differential images is presented. The method involves the subtraction of short movies from each other to create a stack of differential images that can be easily analyzed. In the future, this method will make DMC more accessible and improve throughput.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.
authors.forename: Randall D
authors.initials: RD
authors.lastname: Reif
authors.forename: Kelong
authors.initials: K
authors.lastname: Wang
authors.forename: Dimitri
authors.initials: D
authors.lastname: Pappas
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D006801:Humans