APOC1 T45S polymorphism is associated with reduced obesity indices and lower plasma concentrations of leptin and apolipoprotein C-I in aboriginal Canadians.
Apolipoprotein (apo) C-I is a constituent of chylomicrons, very low density lipoprotein, and high density lipoprotein. The role of apo C-I in human metabolism is incompletely defined. We took advantage of a naturally occurring amino acid polymorphism that is present in aboriginal North Americans, namely apo C-I T45S. We assessed the hypothesis that metabolic traits, including obesity-related and lipoprotein-related traits, would differ between carriers and noncarriers of apo C-I T45S. A genotyping assay was developed for APOC1 T45S and genotypes were determined in a sample of 410 Canadian Oji-Cree subjects. The allele frequency of the apo C-I S45 allele was approximately 8% in this sample. We observed the apo C-I S45 allele was significantly associated with 1) lower percent body fat (P < 0.05), 2) lower waist circumference (P = 0.058), 3) lower serum leptin levels (P < 0.05), and 4) lower plasma apo C-I levels (P < 0.0001), using a newly developed ELISA-based method. Taken together, these results suggest that at the whole human phenotype level, apo C-I is associated with the complex metabolic trait of obesity as well as with serum leptin levels.
Lahiry
Piya
P
Robarts Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.
Cao
Henian
H
Ban
Matthew R
MR
Pollex
Rebecca L
RL
Mamakeesick
Mary
M
Zinman
Bernard
B
Harris
Stewart B
SB
Hanley
Anthony J G
AJ
Huff
Murray W
MW
Connelly
Philip W
PW
Hegele
Robert A
RA
D000293:Adolescent; D000328:Adult; D053302:Apolipoprotein C-I / Q000097:blood* / Q000235:genetics*; D001823:Body Composition; D002170:Canada; D002648:Child; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D015228:Hypertriglyceridemia / Q000453:epidemiology; D007198:Indians, North American / Q000235:genetics*; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood / Q000235:genetics*; D056128:Obesity, Abdominal / Q000453:epidemiology; D011110:Polymorphism, Genetic; D015995:Prevalence; D012727:Sex Characteristics; D055105:Waist Circumference; D055815:Young Adult
C505803:APOC1 protein, human; D053302:Apolipoprotein C-I; D020738:Leptin