[Influence of combination of extractum Angelicae Dahuricae Siccum and total alkaloids of Rhizoma Corydalis on pharmacokinetics of tetrahydropalmatine in rats].
This paper is aimed to develop a rapid and sensitive HPLC-fluorescence detection (FLD) method for the determination of tetrahydropalmatine (TET) in rats' plasma. The influence of combinations of Extractum Angelicae Dahuricae Siccum (coumarin and volatile oil) and total alkaloids (TA) from Rhizoma Corydalis (TA) on pharmacokinetics of TET in rats was studied. Plasma samples were treated with hexane-isopropanol (95:5) to precipitate the protein, and were determined by HPLC-fluorescence detection. The calibration curve was linear in the range of 2.096-167.68 microg L(-1). The limit of quantification was 2.096 microg L(-1). The method recovery of TET was 94.0%-100.0%. The extract recovery was 72.0%-81.5%. RSDs ofintra- and inter-day precisions were all less than 7.0%. Pharmacokinetics of TET in rats was fitted to two compartments open model after oral administration of TA, TA-volatile oil (VO), TA-coumarin (Cou) and TA-VO-Cou. Compared with TA, AUC(0-t), AUC(0-infinity), MRT(0-t), and MRT(0-infinity) of TET had significant deviation when combined with VO and/or Cou. The determination method is sensitive, specific, accurate, and appropriate for determination of TET in vivo. Coumarin and/or VO combined with TA can prolong the resistance time of TET significantly, delay elimination and enhance bioavailability of tetrahydropalmatine.
Liang
Xin-Li
XL
Key Laboratory of Modern Preparation of Traditional Chinese Medicine of Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Liao
Zheng-Gen
ZG
Wang
Guang-Fa
GF
Zhao
Guo-Wei
GW
Dai
Chun-Lan
CL
Zhang
Xiao-Hui
XH
D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D029969:Angelica / Q000737:chemistry; D000818:Animals; D001600:Berberine Alkaloids / Q000493:pharmacokinetics*; D031311:Corydalis / Q000737:chemistry; D003374:Coumarins / Q000008:administration & dosage / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D009822:Oils, Volatile / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry
D000470:Alkaloids; D001600:Berberine Alkaloids; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; C014215:tetrahydropalmatine; C030123:coumarin