Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: effect of organic solvent and initial water content.
The effect of the reaction conditions on the ester synthesis reaction with CALB displaying yeast whole cells was determined. Utilization of hydrophobic organic solvent improved the efficiency of the ester synthesis reaction. Also the initial water content was important for the expression of the ester synthesis activity of CALB displaying yeast whole cells.
Tanino
Takanori
T
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
Ohno
Takumi
T
Ogino
Chiaki
C
Fukuda
Hideki
H
Kondo
Akihiko
A
D002384:Catalysis; D018929:Cell Culture Techniques / Q000379:methods; D004951:Esterification; D004952:Esters / Q000378:metabolism; D005656:Fungal Proteins; D008049:Lipase / Q000378:metabolism*; D009930:Organic Chemicals / Q000378:metabolism*; D012997:Solvents / Q000378:metabolism; D014867:Water / Q000378:metabolism*
D004952:Esters; D005656:Fungal Proteins; D009930:Organic Chemicals; D012997:Solvents; D014867:Water; D008049:Lipase; C431349:lipase B, Candida antarctica