Interleukin-2 induces the activities of DNA topoisomerase I and DNA topoisomerase II in HuT 78 cells.
The induction by interleukin-2 of DNA topoisomerase I and DNA topoisomerase II activities in the human T cell line HuT 78 was investigated. HuT 78 cells were treated with 1000 U of interleukin-2/ml, and extracts of the HuT 78 nuclei were prepared over a 24h period. The extracts were assayed quantitatively for the activities of DNA topoisomerase I and DNA topoisomerase II. Three concomitant, transient increases of 3- to 11-fold in the specific activities of both DNA topoisomerase I and DNA topoisomerase II were observed following treatment with IL-2 at 0.5, 4, and 10h after treatment with interleukin-2. The specific activities of both enzymes returned to base-line values after each of these transient increases. These results reveal that the activities of DNA topoisomerase I and DNA topoisomerase II are highly regulated in HuT 78 cells upon treatment with IL-2.
Pham
Minh-Hieu
MH
Department of Biology, UPO #311, University of the Incarnate Word, 4301 Broadway, San Antonio, TX 78209-6318, USA. minhhieu21@att.net
Kondapalli
Navya
N
Reckord
Calvin L
CL
Foglesong
P David
PD
D045744:Cell Line, Tumor; D004264:DNA Topoisomerases, Type I / Q000032:analysis / Q000096:biosynthesis*; D004250:DNA Topoisomerases, Type II / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000201:enzymology
D007376:Interleukin-2; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II