Pancreas-specific protein disulfide isomerase has a cell type-specific expression in various mouse tissues and is absent in human pancreatic adenocarcinoma cells: implications for its functions.
Members of the protein disulfide isomerase (PDI) family play a critical role in catalyzing the formation of disulfide bonds in secretory proteins, and most of these enzymes have a wide tissue distribution. However, the pancreas-specific PDI homolog was previously suggested to be exclusively expressed in the pancreas (thus commonly referred to as PDIp). In the present study, we found that PDIp was also highly expressed in several other tissues in mice, including the stomach, cecum, ileum, adrenal glands, epididymis, and prostate. Notably, in the digestive organs, such as the stomach and pancreas, very high levels of PDIp were selectively expressed in the digestive enzyme-secreting cells (e.g., gastric chief cells and pancreatic acinar cells). This observation suggests that PDIp may function as a protein-folding catalyst for secretory digestive enzymes. In ileum, PDIp was exclusively expressed in Paneth cells. In addition, high levels of PDIp expression were also detected in normal human pancreas, but its expression was mostly absent in human pancreatic duct adenocarcinoma and pancreatic cancer cell lines. The absence of PDIp expression in pancreatic adenocarcinoma may serve as an additional biomarker for pancreatic cancer.
Fu
Xin-Miao
XM
Department of Pharmacology, Toxicology and Therapeutics, School of Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Dai
Xiangchen
X
Ding
Jian
J
Zhu
Bao Ting
BT
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D021441:Carcinoma, Pancreatic Ductal / Q000201:enzymology / Q000473:pathology; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology* / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000473:pathology; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D021381:Protein Transport; D013347:Subcellular Fractions / Q000201:enzymology
D019704:Protein Disulfide-Isomerases