Identification of a synthetic peptide inducing cross-reactive antibodies binding to Rhipicephalus (Boophilus) decoloratus, Rhipicephalus (Boophilus) microplus, Hyalomma anatolicum anatolicum and Rhipicephalus appendiculatus BM86 homologues.
The BM86 antigen, originally identified in Rhipicephalus (Boophilus) microplus, is the basis of the only commercialized anti-tick vaccine. The long-term goal of our study is to improve BM86 based vaccines by induction of high levels of tick gut binding antibodies that are also cross-reactive with a range of BM86 homologues expressed in other important tick species. Here we have used a BD86 derived synthetic peptide, BD86-3, to raise a series of mouse monoclonal antibodies. One of these mAbs, named 12.1, recognized BM86 homologues in immuno-histochemical analyses in four out of five tick species including R. (B.) microplus, Rhipicephalus (Boophilus) decoloratus, Hyalomma anatolicum anatolicum and Rhipicephalus appendiculatus. Our results indicate that broadly cross-reactive tick gut binding antibodies can be induced after immunization with a synthetic peptide derived from the protein BD86.
Kopp
Nadja
N
Swiss Tropical Institute, Department of Medical Parasitology and Infection Biology, Socinstrasse 57, 4002 Basel, Switzerland.
Diaz
Diana
D
Amacker
Mario
M
Odongo
David O
DO
Beier
Konstantin
K
Nitsch
Cordula
C
Bishop
Richard P
RP
Daubenberger
Claudia A
CA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006825:Hybridomas; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D048494:Rhipicephalus / Q000276:immunology; D016415:Sequence Alignment; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D022721:Vaccines, Virosome / Q000276:immunology
D000911:Antibodies, Monoclonal; C085065:BM86 protein, Boophilus; D008562:Membrane Glycoproteins; D010455:Peptides; D011994:Recombinant Proteins; D014612:Vaccines; D022223:Vaccines, Subunit; D022721:Vaccines, Virosome