Different suppressive effects of fucoidan and lentinan on IL-8 mRNA expression in in vitro gut inflammation.
A system for assessing the anti-inflammatory effects of food factors was developed by establishing a co-culture system with intestinal epithelial Caco-2 cells and macrophage RAW264.7 cells. The results indicate that fucoidan and lentinan exhibited different suppressive effects on interleukin-8 (IL-8) mRNA expression in Caco-2 through tumor necrosis factor-alpha (TNF-alpha) production from RAW264.7 stimulated with lipopolysaccharide (LPS).
Mizuno
Masashi
M
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Japan. mizuno@kobe-u.ac.jp
Nishitani
Yosuke
Y
Hashimoto
Takashi
T
Kanazawa
Kazuki
K
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D018938:Caco-2 Cells; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics*; D007912:Lentinan / Q000494:pharmacology* / Q000627:therapeutic use; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000893:Anti-Inflammatory Agents; D016209:Interleukin-8; D011134:Polysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007912:Lentinan; C007789:fucoidan