[TLR4 signals are involved in multiple myeloma cell proliferation and apoptosis].
To investigate the TLR4 signaling in multiple myeloma cell proliferation and apoptosis.



TLR4 gene transcription and protein expression were detected by RT-PCR and PE flow cytometry staining; the cells proliferation were detected by MTT assay; doxorubicin-induced apoptosis of cells was detected by AnnexinV-PI double staining flow cytometry.



TLR4 gene transcription and protein expression was detected in the myeloma cell lines. Under the lipopolysaccharides (LPS) stimulation, MM1-s cells TLR4 positive proliferation significantly increased (P<0.05), but not found in U266 cells TLR4 negative; MM1-s cells showed the resistance to doxorubisin-induced apoptosis, but LPS did not protect doxorubicin-induced U266 cell apoptosis.



TLR4 signaling may play an important role both in multiple myeloma proliferation and survival.
Bao
Han-Ying
HY
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Wang
Li-Juan
LJ
Yang
Yang
Y
Cai
Zhen
Z
D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4