Cardiac biomarkers in a model of acute catecholamine cardiotoxicity.
Coronary heart disease and in particular its most serious form - acute myocardial infarction (AMI) - represents the most common cause of mortality in developed countries. Better prognosis may be achieved by understanding the etiopathogenetic mechanisms of AMI. Therefore, a catecholamine model of myocardial injury, which has appeared to be very similar to AMI in human in some aspect, was used. Male Wistar:Han rats were randomly divided into two groups: control group (saline) and isoprenaline group (ISO; synthetic catecholamine, 100 mg.kg(- 1) subcutaneously [s.c.]). After 24 hours, functional parameters were measured, biochemical markers in the blood and metals content in the heart tissue were analysed and histological examination was performed. ISO caused marked myocardial injury that was associated with myocardial calcium overload. Close correlation between myocardial impairment (i.e. serum TnT, stroke volume index and wet ventricles weight) and the levels of myocardial calcium was observed. Direct reactive oxygen species (ROS) involvement was documented only by non-significant increase in malonyldialdehyde 24 hours after ISO injury. Moreover, myocardial element analysis revealed no significant changes as for the content of zinc and iron while selenium and copper increased in the ISO group although it reached statistical significance only for the latter.
Mladenka
Premysl
P
Department of Pharmacology and Toxicology, Charles University in Prague, Hradec Králové, Czech Republic. mladenkap@faf.cuni.cz
Hrdina
Radomír
R
Bobrovová
Zuzana
Z
Semecky
Vladimír
V
Vávrová
Jaroslava
J
Holecková
Magdaléna
M
Palicka
Vladimir
V
Mazurová
Yvona
Y
Nachtigal
Petr
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood; D015415:Biomarkers / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002395:Catecholamines / Q000633:toxicity*; D004195:Disease Models, Animal; D006334:Heart Function Tests; D006339:Heart Rate / Q000187:drug effects; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007501:Iron / Q000378:metabolism; D007545:Isoproterenol / Q000633:toxicity*; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D020107:Troponin T / Q000097:blood; D014810:Vitamin E / Q000097:blood; D015032:Zinc / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D002395:Catecholamines; D017382:Reactive Oxygen Species; D020107:Troponin T; D014810:Vitamin E; D007501:Iron; D015032:Zinc; D007545:Isoproterenol; D001205:Ascorbic Acid; D002118:Calcium