title: Validation of immunoassays for the candidate renal markers C-reactive protein, immunoglobulin G, thromboxane B2 and retinol binding protein in canine urine.
abstract: The study of early markers for glomerular and tubular dysfunction in dogs with renal diseases holds promise to gain new insights in the pathogenesis of canine nephropathies. However, the validation of such markers in canine urine is largely lacking. Therefore, immunoassays for the quantification of a set of four urinary markers, C-reactive protein (CRP), immunoglobulin G (IgG), thromboxane B(2) (TXB(2)) and retinol binding protein (RBP), were validated by determining their sensitivity, reproducibility, precision and accuracy in a large patient group. The results show that the immunoassays are appropriate for analysis of urinary CRP, IgG, TXB(2) and RBP in dogs. Furthermore, the significant differences in urinary concentrations of the selected glomerular and tubular markers between healthy (H) dogs and dogs with several types of nephropathies (R) support their future application in both clinical settings and research models.
authors.affiliation: Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. bert.maddens@ugent.be
authors.forename: Bert E J
authors.initials: BE
authors.lastname: Maddens
authors.forename: Sylvie
authors.initials: S
authors.lastname: Daminet
authors.forename: Kristel
authors.initials: K
authors.lastname: Demeyere
authors.forename: Dieter
authors.initials: D
authors.lastname: Demon
authors.forename: Pascale
authors.initials: P
authors.lastname: Smets
authors.forename: Evelyne
authors.initials: E
authors.lastname: Meyer
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D002097:C-Reactive Protein / Q000652:urine*; D016022:Case-Control Studies; D004283:Dog Diseases / Q000652:urine; D004285:Dogs / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D007074:Immunoglobulin G / Q000652:urine*; D007668:Kidney / Q000378:metabolism; D007674:Kidney Diseases / Q000652:urine / Q000662:veterinary; D012177:Retinol-Binding Proteins / Q000652:urine*; D013929:Thromboxane B2 / Q000652:urine*
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D012177:Retinol-Binding Proteins; D013929:Thromboxane B2; D002097:C-Reactive Protein