Exploring natural anticoagulation by endothelial cells: a novel in vitro model.
Acute damage and activation of endothelial cells (EC), the inner lining of blood vessels, is linked to release of heparan sulfate proteoglycans and exposure of a proinflammatory and procoagulant cell surface. To study the pathophysiology of EC activation and the effect of substances which protect EC in vitro we have developed a model with EC cultured on small beads, which are then incubated with whole, non-anticoagulated human blood.
Banz
Yara
Y
Department of Clinical Research, Cardiovascular Research, University of Bern, Switzerland.
Rieben
Robert
R
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001769:Blood; D001777:Blood Coagulation / Q000502:physiology*; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D019812:Heparan Sulfate Proteoglycans / Q000502:physiology; D006801:Humans; D007249:Inflammation / Q000503:physiopathology*
D000925:Anticoagulants; D019812:Heparan Sulfate Proteoglycans