Fluorescence-based detection of point mutation in DNA sequences by CdS quantum dot aggregation.
We present a novel method for the detection of single base mismatch based on fluorescence quenching that unmodified CdS quantum dots exhibit upon aggregation. Target DNA sequences of interest are breast cancer 2 (BRCA2) and signal-induced proliferation-associated gene 1 (Sipa1) sequences. We monitor aggregation of CdS quantum dots upon addition of double-stranded DNAs at different salt concentration using quasi-elastic light scattering (QELS), transmission electron microscopy (TEM), photoluminescence spectroscopy, and zeta potential measurement. Our results indicate that the double-stranded DNA with a perfectly matched sequence can easily be discerned by naked eye from the single base mismatched one due to the fluorescence quenching phenomenon caused by selective aggregation of the CdS quantum dots.
Kim
Taehoon
T
Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Korea.
Noh
Minho
M
Lee
Hosub
H
Joo
Sang-Woo
SW
Lee
So Yeong
SY
Lee
Kangtaek
K
D024682:BRCA2 Protein / Q000235:genetics; D019187:Cadmium Compounds / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D020690:GTPase-Activating Proteins / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D017354:Point Mutation; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000737:chemistry*
D024682:BRCA2 Protein; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D020690:GTPase-Activating Proteins; D013440:Sulfides; C034939:cadmium sulfide; D004247:DNA