[The study of changes on NKT cells of experimental autoimmune encephalomyelitis (EAE) mice].
OBJECTIVE
To observe the changes of the number of NKT cells in spleens and livers of induced model of experimental autoimmune encephalomyelitis (EAE), and to study the role NKT cells play in the immunoregulation of EAE.


METHODS
C57BL/6 mice were immunized with MOG (35-55) peptide and received clinical evaluation daily. The mice were sacrificed at the fastigium and the splenic and hepatic lymphocytes were isolated. The changes of NKT cells in normal and EAE C57BL/6 mice were detected by flow cytometry.


RESULTS
The percent of NKT cells in lymphocytes of different organs of EAE model were greater decreased than in that of normal mice. The percent of NKT cells in splenic lymphocytes of normal mice was 2.22+/-0.14, while that in EAE mice was 1.94+/-0.07 (P<0.05). The percent of NKT cells in hepatic lymphocytes of normal mice was 5.52+/-2.17, while that in EAE mice was 2.67+/-1.41 (P<0.05).


CONCLUSIONS
The proliferation of splenic and hepatic NKT cells in C57BL/6 mice are inhibited in EAE model, which may indicate that the immune function conducted by NKT cell is down regulated in EAE mice.
Ouyang
Qing
Q
Department of Stomatology Lochus 6, the Fourth Military Medical University, Xi'an 710032, China. ouyangqing-hellen@163.com
Chen
Kun
K
Wang
Xi
X
Zhang
Chun-Mei
CM
Guo
Jun
J
Wei
Yu-Ying
YY
Sun
Yuan-Jie
YJ
Xu
Zhu-Wei
ZW
Yang
Kun
K
D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000166:cytology; D051379:Mice; D008969:Molecular Sequence Data; D055611:Natural Killer T-Cells / Q000166:cytology* / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D013154:Spleen / Q000166:cytology
D010446:Peptide Fragments