Timing of serum soluble HLA-G levels in acute and subacute phases after spontaneous intracerebral hemorrhage.
Serum levels of sHLA-G (sHLA-G1/HLA-G5) antigens and their soluble isoforms, sHLA-G1 and HLA-G5, were measured by ELISA in 22 patients with spontaneous intracerebral hemorrhage (SICH) at 24 h, 48 h and 7 days after bleeding. The perihematomal edema volume was calculated on non-enhanced computed tomography scans using the formula AxBxC/2 at the same time points. The mean serum concentrations of sHLA-G1/HLA-G5 and sHLA-G1 as well as the perihematomal edema volume changed significantly over time (p < 0.0001, p < 0.001 and p < 0.0001, respectively), whereas no statistical differences were found in serum HLA-G5 concentrations over the course of the experiment. In comparison to the values found at 24 h, sHLA-G1/HLA-G5 and sHLA-G1 increased at 48 h and then decreased at 7 days, whereas the perihematomal edema volume was more elevated at 48 h and, to a lesser extent, at 7 days. A positive correlation was detected between mean serum sHLA-G1/HLA-G5 and sHLA-G1 levels and perihematomal edema volume at 24 h (p < 0.02) and at 48 h (p < 0.01). Our results may indicate a role for sHLA-G in inflammatory mechanisms related to SICH, where these proteins probably act as anti-inflammatory molecules and are predominantly produced as the sHLA-G1 isoform.
Fainardi
Enrico
E
Neuroradiology Unit, Department of Neurosciences and Rehabilitation, Azienda Ospedaliera-Universitaria, Ferrara, Italy.
Rizzo
Roberta
R
Lupato
Angelica
A
Ramponi
Vania
V
De Santis
Giuseppe
G
Garofano
Francesco
F
Stignani
Marina
M
Tamborino
Carmine
C
Castellazzi
Massimiliano
M
Casetta
Ilaria
I
Baricordi
Olavio Roberto
OR
D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001929:Brain Edema / Q000209:etiology; D002543:Cerebral Hemorrhage / Q000097:blood* / Q000150:complications / Q000000981:diagnostic imaging; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006680:HLA Antigens / Q000097:blood*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014057:Tomography, X-Ray Computed / Q000379:methods
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I