Analysis of mycophenolic acid in dried blood spots using reversed phase high performance liquid chromatography.
At our laboratory a reversed phase high performance liquid chromatography assay was developed for analysis of mycophenolic acid in dried blood spot samples. The assay was validated in the range of 0.74-23.4mg/L and proved to be accurate and precise. The developed sample pretreatment procedure was consistent and has a recovery of 95.2% for mycophenolic acid. Hematocrit showed to have influence on the physics of the blood spots and thus on the concentration of mycophenolic acid, therefore standard and control samples should be made with a standardized hematocrit.
Wilhelm
A J
AJ
VU Medical Center, Laboratory of Pharmacology and Pharmacy, Boelelaan 1117, 1081 HV Amsterdam, Netherlands.
den Burger
J C G
JC
Chahbouni
A
A
Vos
R M
RM
Sinjewel
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004355:Drug Stability; D009173:Mycophenolic Acid / Q000097:blood*
D009173:Mycophenolic Acid