[Detection of LY6G6D gene exon-intron structure,].
Earlier we have shown high frequency of loss of heterozygosity of microsatellite marker D6S273 within HLA III class region in DNA samples from cervical intraepithelial neoplasias and cervical cancer. According to publications three genes were identified in this region. For detection of D6S273 position we used in silico analysis of mRNA sequences deposited in GenBank (NCBI) and investigated LY6G6D gene expression in tumor cell lines. LY6G6D gene exon borders were analyzed with 5'- or 3'-rapid amplification of cDNA ends. We have found that LY6G6D gene consists of 9 exons and includes two earlier identified genes G6D and G6F. Microsatellite D6S273 is located in the last 8 intron of LY6G6D gene. The third gene LY6G6E consisting of four exons is located in 6 intron of LY6G6D gene in the opposite orientation. We suggest that LY6G6D gene is coding three main mRNA transcripts in the same open reading frame but differ in exon composition: MEGT1 consists of 1-4, 8, 9 exons, G6F consists of 1-6 exons and G6D consists of 7-9 exons of LY6G6D gene. High homology with immunoglobulin superfamily within 20-120 aminoacids of MEGT1 and G6F proteins is shown by in silico translation of their mRNAs.
Beliakov
I S
IS
Karakasheva
T A
TA
Mazurenko
N N
NN
D017398:Alternative Splicing / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005091:Exons / Q000235:genetics*; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D019656:Loss of Heterozygosity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics
D006680:HLA Antigens; D007136:Immunoglobulins; C548380:LY6G6D protein, human; D012333:RNA, Messenger