Aggregating brain cell cultures: investigation of stroke related brain damage.
Aggregating brain cell cultures are three-dimensional primary cell cultures derived from embryonal rat brain cells. Within 3-4 weeks in culture under continuous agitation, they exhibit organotypic structures and functions. The transient arrest of agitation induced adverse effects comparable with those occurring in the ischemic brain in vivo. This culture system therefore offers a suitable in vitro model for the elucidation of ischemia-related pathogenic processes in the brain.
Honegger
Paul
P
Department of Physiology, University of Lausanne, Lausanne, Switzerland. paul.honegger@unil.ch
Pardo
Beatriz
B
D000826:Animal Testing Alternatives; D000818:Animals; D001921:Brain / Q000196:embryology / Q000473:pathology*; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000379:methods; D016923:Cell Death; D006801:Humans; D051379:Mice; D008875:Middle Aged; D051381:Rats; D012307:Risk Factors; D020521:Stroke / Q000453:epidemiology / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology