title: Simultaneous quantification of 8-iso-prostaglandin-F(2alpha) and 11-dehydro thromboxane B(2) in human urine by liquid chromatography-tandem mass spectrometry.
abstract: Both F(2)-isoprostanes (8-iso-PGF(2alpha)), a well-known marker of oxidative stress, and thromboxanes A(2) (TXA(2)) are involved in atherosclerosis through LDL oxidation and platelet activation. Different aspects of the pathology can be described by 8-iso-PGF(2alpha) and TXA(2) so it is important to determine both their concentrations to monitor the disease progression and/or therapy effects. We developed a simple and sensitive method based on liquid chromatography-tandem mass spectrometry, using electrospray ionization in negative-ion mode, for the simultaneous measurement of the concentration of 8-iso-PGF(2alpha) and 11-dehydro thromboxane B(2) (11-DH-TXB(2)), a TXA(2) metabolite. This method was applied to analyze urine samples collected overnight from 15 atherosclerotic patients, with documented carotid artery sclerosis (CAS), and from 20 controls. The detection limit was 0.097pg/microL for 8-iso-PGF(2alpha) and 0.375pg/microL for 11-DH-TXB(2), with a linear range of 0.78-25pg/microL; the inter- and intraday imprecision was <5% for both metabolites. These analytes were higher in CAS (P<0.005 vs controls) and were positively correlated in patients but not in controls, even after adjustment for age and gender (r=0.60; P=0.032). This highly sensitive, precise, and rapid method allows for the simultaneous determination of 8-iso-PGF(2alpha) and 11-DH-TXB(2) in human urine samples in order to evaluate oxidative stress and platelet aggregation.
authors.affiliation: Università degli Studi di Milano, Dipartimento di Scienze Cardiovascolari, 20138 Milano, Italy. viviana.cavalca@unimi.it
authors.forename: Viviana
authors.initials: V
authors.lastname: Cavalca
authors.forename: Fabiana
authors.initials: F
authors.lastname: Minardi
authors.forename: Samuele
authors.initials: S
authors.lastname: Scurati
authors.forename: Federico
authors.initials: F
authors.lastname: Guidugli
authors.forename: Isabella
authors.initials: I
authors.lastname: Squellerio
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Veglia
authors.forename: Luca
authors.initials: L
authors.lastname: Dainese
authors.forename: Anna
authors.initials: A
authors.lastname: Guarino
authors.forename: Elena
authors.initials: E
authors.lastname: Tremoli
authors.forename: Donatella
authors.initials: D
authors.lastname: Caruso
mesh_terms: D000368:Aged; D050197:Atherosclerosis / Q000652:urine*; D015415:Biomarkers / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D010974:Platelet Aggregation / Q000502:physiology; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods; D013928:Thromboxane A2 / Q000378:metabolism; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000652:urine
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D013929:Thromboxane B2; D013928:Thromboxane A2; C049235:11-dehydro-thromboxane B2; D015237:Dinoprost