Tissue-specific distribution of uncoupling proteins in normal rats and rats with high-fat-diet-induced obesity.
Uncoupling proteins (UCPs) are a proton transporter family located in the mitochondrial inner membrane. Thus far, five molecules (UCP1-UCP5) have been identified as members of the UCP family. Recently, UCPs have attracted considerable interest in research on energy metabolism and obesity. However, to date, no study has focused on a comprehensive and systematic evaluation of the tissue-specific distribution of UCPs in obese individuals. Our study presents evidence of differential tissue expression profiles of five isoforms of UCPs in normal and diet-induced obese (DIO) rats using real-time polymerase chain reaction (PCR) analysis. The results clearly show that the tissue-specific expression patterns of individual isoforms between DIO and normal rats are quite distinct, which suggests a close relationship between the alterations in UCP expression and dietary obesity.
Zhang
Chun-Mei
CM
Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing, China.
Gu
Ying
Y
Qing
Da-ni
DN
Zhu
Jin-gai
JG
Zhu
Chun
C
Zhang
Min
M
Guo
Xi-Rong
XR
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism*; D009765:Obesity / Q000097:blood / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012187:Retroperitoneal Space; D014280:Triglycerides / Q000097:blood; D000071256:Uncoupling Protein 1; D015430:Weight Gain
D001786:Blood Glucose; D004041:Dietary Fats; D007473:Ion Channels; D008077:Lipoproteins, LDL; D024101:Mitochondrial Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D014280:Triglycerides; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D002784:Cholesterol