title: Swine influenza matrix 2 (M2) protein contributes to protection against infection with different H1 swine influenza virus (SIV) isolates.
abstract: A swine influenza virus (SIV) vaccine-challenge pig model was used to study the potential of a conserved matrix 2 (M2) protein vaccine alone or in combination with an inactivated H1N1-vaccine to protect against H1N1 and H1N2 viruses. The H1N1-vaccine and heterologous H1N2-challenge virus model has previously been shown to prolong fever and increase SIV-associated pneumonic lesions. The M2 vaccine in combination with the H1N1-vaccine reduced the H1N2 induced fever but not virus shedding. The M2 vaccine alone reduced respiratory signs and pneumonic lesions to levels similar to the negative control pigs following H1N2 infection. This study found that the M2 protein has potential as a vaccine for SIV-associated disease prevention. However, development of an immune response towards the major envelope HA protein was required to reduce SIV shedding.
authors.affiliation: College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: Pravina
authors.initials: P
authors.lastname: Kitikoon
authors.forename: Amy L
authors.initials: AL
authors.lastname: Vincent
authors.forename: Bruce H
authors.initials: BH
authors.lastname: Janke
authors.forename: B
authors.initials: B
authors.lastname: Erickson
authors.forename: Erin L
authors.initials: EL
authors.lastname: Strait
authors.forename: Shan
authors.initials: S
authors.lastname: Yu
authors.forename: Marie R
authors.initials: MR
authors.lastname: Gramer
authors.forename: Eileen L
authors.initials: EL
authors.lastname: Thacker
mesh_terms: D000818:Animals; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D057221:Influenza A Virus, H1N2 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D013552:Swine; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D007252:Influenza Vaccines; D014759:Viral Envelope Proteins