title: Radioprotective potential of a novel therapeutic formulation of oligoelements Se, Zn, Mn plus Lachesis muta venom.
abstract: In this study we evaluated in vivo the tolerance induced by the combination of Se, Zn and Mn (4 microg/ml each) plus Lachesis muta venom (4 ng/ml) (O-LM) to high doses of ionizing radiation. The protective effect of O-LM was investigated on the small-intestine and bone marrow of mice irradiated with a single whole-body dose of 10 Gy employing a (137)Cs source. Mice were sacrificed 3 days after irradiation. Mice receiving a subcutaneous daily O-LM injection starting 30 days before irradiation, showed a higher number of crypts, enhanced villous conservation and lack of edema or vascular damage in comparison to the untreated and irradiated group. In addition, O-LM treatment decreased vascular damage and the grade of aplasia preserving medullar progenies induced by ionizing radiation on mouse bone marrow. The protective effect of O-LM against radiation injury to the small intestine was associated with an increase in proliferation and a reduction of apoptosis in intestinal crypts and furthermore, to an enhanced intestinal immunoreactivity of MnSOD, and CuZnSOD, and also catalase. Based on the present results and taking into account that O-LM is being safely administered in phase I clinical trial as an immunomodulator, we suggest that O-LM could be an attractive candidate as a safe radioprotective agent for patients undergoing radiotherapy.
authors.affiliation: Institute of Immunooncology, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Crescenti
authors.forename: Máximo
authors.initials: M
authors.lastname: Croci
authors.forename: Vanina
authors.initials: V
authors.lastname: Medina
authors.forename: Lorena
authors.initials: L
authors.lastname: Sambuco
authors.forename: Rosa
authors.initials: R
authors.lastname: Bergoc
authors.forename: Elena
authors.initials: E
authors.lastname: Rivera
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology* / Q000528:radiation effects*; D003435:Crotalid Venoms / Q000008:administration & dosage*; D004338:Drug Combinations; D007421:Intestine, Small / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D019216:Metals, Heavy / Q000008:administration & dosage*; D051379:Mice; D008819:Mice, Nude; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D014916:Whole-Body Irradiation / Q000009:adverse effects*
chemical_list: D003435:Crotalid Venoms; D004338:Drug Combinations; C072644:Lachesis venom; D019216:Metals, Heavy; D011837:Radiation-Protective Agents