Genetic analysis of canine group 2 coronavirus in Korean dogs.
Three out of 109 mixed lung and tracheal tissue extracts originating from Korean dogs tested positive for infection with canine respiratory coronavirus (CRCoV) of Coronaviridae group 2. The three CRCoV-positive samples were found to be competent for viral propagation and isolation, using human rectal tumor cells (HRT-18), and the structure and nonstructure proteins encoded in the 3'-end of the CRCoV genome were sequenced. The small open reading frames situated between the spike and envelope genes of the three Korean CRCoV isolates were found to encode three nonstructural proteins (4.9 kDa, 2.7 kDa, and 12.8 kDa in size), as were the British (CRCoV G9142) and Italian (CRCoV 240-05) strains. Comparison of the deduced amino acid sequences of the spike protein in the CRCoV and bovine coronavirus (BCoV) strains revealed twenty sequence variations. The predicted spike protein of CRCoV contained 20 or 21 N-glycosylation sites, whereas that of BCoV contained 19 sites. Phylogenetic analysis of the spike gene from eight CRCoV and six BCoV strains, performed using the neighbor-joining approach, allowed us to classify into two clades (CRCoV and BCoV) and three Korean strains (CRCoV-K9, -K37, and -K39) related to the Japanese strain 06/075.
An
Dong-Jun
DJ
National Veterinary Research and Quarantine Service, Anyang, Kyunggi-do, 430-824, Republic of Korea.
Jeong
Wooseog
W
Yoon
Sook Hee
SH
Jeoung
Hye-Young
HY
Kim
Hyun-Jeong
HJ
Park
Bong-Kyun
BK
D000818:Animals; D045744:Cell Line, Tumor; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D007723:Korea; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017385:Sequence Homology; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins