title: Sulfated modification and immunomodulatory activity of water-soluble polysaccharides derived from fresh Chinese persimmon fruit.
abstract: In this study, three kinds of chemically sulfated polysaccharides (PFP-S) were derived from a water-soluble polysaccharide of persimmon fruit with chlorosulfonic acid-pyridine method. Relationship between the degree of substitution and immunomodulatory activity of PFP-S was examined with the splenocytes experiment. The results showed that the splenocytes-activating activity was significantly enhanced by PFP-S in all the groups compared with control group (P<0.01). PFP-SII exhibited the most potent splenocytes-activating activity by increased cytokine production and NO release. It also suggested that the sulfate groups and molecular weight of polysaccharides are key factors to regulate the immunomodulatory activities.
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xianning Road 28#, Xi'an 710049, Shaanxi Province, PR China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lu
authors.forename: Yuning
authors.initials: Y
authors.lastname: Zhang
authors.forename: Liguo
authors.initials: L
authors.lastname: Qin
authors.forename: Jianbao
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002681:China; D029788:Diospyros / Q000737:chemistry*; D005260:Female; D005638:Fruit / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D007155:Immunologic Factors / Q000276:immunology* / Q000494:pharmacology; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012995:Solubility / Q000187:drug effects; D013055:Spectrophotometry, Infrared; D013154:Spleen / Q000166:cytology; D013431:Sulfates / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014867:Water
chemical_list: D007155:Immunologic Factors; D007376:Interleukin-2; D011134:Polysaccharides; D012333:RNA, Messenger; D013431:Sulfates; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D009569:Nitric Oxide