title: Radioprotection by short-term oxidative preconditioning: role of manganese superoxide dismutase.
abstract: Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H(2)O(2) increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD.
authors.affiliation: Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Belikova
authors.forename: Ashley
authors.initials: A
authors.lastname: Glumac
authors.forename: Ruslan
authors.initials: R
authors.lastname: Rafikov
authors.forename: Jianfei
authors.initials: J
authors.lastname: Jiang
authors.forename: Joel S
authors.initials: JS
authors.lastname: Greenberger
authors.forename: Valerian E
authors.initials: VE
authors.lastname: Kagan
authors.forename: Hülya
authors.initials: H
authors.lastname: Bayir
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors; D004622:Embryo, Mammalian / Q000166:cytology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D012402:Rotenone; D000968:Antimycin A; D013482:Superoxide Dismutase; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III