Bacterial chemoattraction towards jasmonate plays a role in the entry of Dickeya dadantii through wounded tissues.
Jasmonate is a key signalling compound in plant defence that is synthesized in wounded tissues. In this work, we have found that this molecule is also a strong chemoattractant for the phythopathogenic bacteria Dickeya dadantii (ex-Erwinia chysanthemi). Jasmonic acid induced the expression of a subset of bacterial genes possibly involved in virulence/survival in the plant apoplast and bacterial cells pre-treated with jasmonate showed increased virulence in chicory and Saintpaulia leaves. We also showed that tissue wounding induced bacterial spread through the leaf surface. Moreover, the jasmonate-deficient aos1 Arabidopsis thaliana mutant was more resistant to bacterial invasion by D. dadantii than wild-type plants. These results are consistent with the hypothesis that sensing jasmonic acid by this bacterium helps the pathogen to ingress inside plant tissues.
Antunez-Lamas
Maria
M
Centro de Biotecnología y Genómica de Plantas (CBGP), Departamento de Biotecnología. E. T. S. Ingenieros Agrónomos. Universidad Politécnica de Madrid, 28040 Madrid, Spain.
Cabrera
Ezequiel
E
Lopez-Solanilla
Emilia
E
Solano
Roberto
R
González-Melendi
Pablo
P
Chico
Jose Manuel
JM
Toth
Ian
I
Birch
Paul
P
Pritchard
Leighton
L
Prichard
Leighton
L
Liu
Hui
H
Rodriguez-Palenzuela
Pablo
P
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects*; D018651:Cichorium intybus / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D003517:Cyclopentanes / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism; D019684:Magnoliopsida / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D054883:Oxylipins / Q000494:pharmacology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D010944:Plants / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
D001426:Bacterial Proteins; D003517:Cyclopentanes; D007506:Iron-Sulfur Proteins; D054883:Oxylipins; D012329:RNA, Bacterial; D012097:Repressor Proteins; D037521:Virulence Factors; C011006:jasmonic acid; D013437:Carbon-Sulfur Lyases