title: Localization of microcystin-LR in medaka fish tissues after cyanotoxin gavage.
abstract: Microcystins (MCs) are toxic monocyclic heptapeptides produced by many cyanobacteria. Over 70 MCs have been successfully isolated and identified, of which MC-LR is the most commonly occurring toxin. Microcystins, especially MC-LR, cause toxic effects in mammals, birds and fish and are a recognized potent cause of environmental stress and pose a potential health hazard in aquatic ecosystems when heavy blooms of cyanobacteria appear. They also constitute a public health threat to people via drinking water and food chains. The concentrations of MC-LR can be very low, even in fish displaying severely disrupted tissues, which makes it essential to devise selective and sensitive histochemical methods for identifying and localizing MC-LR in target organs, such as liver and intestine. The aim of the study reported here was to analyze the presence of MC-LR in contaminated fish tissues using immunohistochemical methods. The present experiment involving subacute exposure confirmed our initial hypothesis that subacute and acute exposure to microcystin contamination can exacerbate physiological stress, induce sustained pathological damage, and affect the immune response in exposed medaka fish.
authors.affiliation: FRE 3206 CNRS-USM 0505 Molécules de communication et adaptation des micro-organismes, 12 rue Buffon, Muséum National d'Histoire Naturelle, F-75231 Paris cedex 05, France.
authors.forename: Chakib
authors.initials: C
authors.lastname: Djediat
authors.forename: Mélodie
authors.initials: M
authors.lastname: Malécot
authors.forename: Amaury
authors.initials: A
authors.lastname: de Luze
authors.forename: Cécile
authors.initials: C
authors.lastname: Bernard
authors.forename: Simone
authors.initials: S
authors.lastname: Puiseux-Dao
authors.forename: Marc
authors.initials: M
authors.lastname: Edery
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D007441:Intubation, Gastrointestinal; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008387:Marine Toxins; D052998:Microcystins / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity; D009990:Oryzias / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D013347:Subcellular Fractions / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR