Evaluation of a new polymeric stationary phase with reversed-phase properties for high temperature liquid chromatography.
The performance of a polymeric stationary phase with reversed-phase properties (ET-RP1) was evaluated for LC separations at elevated temperature. The most significant observation was that the reduced plate height (h) decreased from 3.4 at 25 degrees C (optimal flow 0.5 mL/min) to 2.4 at 150 degrees C (optimal flow 2.5 mL/min) which is comparable to the efficiency obtained with silica-based reversed-phase columns of 4.6mm ID operated at 0.8 mL/min. The phase showed no deterioration after long use at 150 degrees C within the pH range 1-9. Catalytic activity originating from the stationary phase material, e.g. as experienced on zirconium columns operated at elevated temperature, was absent. The performance of ET-RP1 is illustrated with the analysis of some pharmaceutical samples by LC and LC-MS. Operation at elevated temperature also allows to reduce the amount of organic modifier or to replace acetonitrile and methanol by the biodegradable ethanol.
Vanhoenacker
Gerd
G
Research Institute for Chromatography, Kennedypark 26, B-8500 Kortrijk, Belgium.
Dos Santos Pereira
Alberto
A
Kotsuka
Takashi
T
Cabooter
Deirdre
D
Desmet
Gert
G
Sandra
Pat
P
D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D004364:Pharmaceutical Preparations; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results
D000097:Acetonitriles; D004364:Pharmaceutical Preparations; D011108:Polymers; D000432:Methanol; C032159:acetonitrile