title: Sequestration, fluorometric detection, and mass spectroscopy analysis of lanthanide ions using surface modified magnetic microspheres for microfluidic manipulation.
abstract: Several methods for rapid sequestration, fluorometric detection, and the subsequent mass spectroscopic analysis of lanthanide ions using surface modified polystyrene magnetic microspheres are demonstrated. Mixed-ligand antenna complexes of Eu(3+) in which one of the ligands is attached to the surface of the microspheres have been used as a means for the sequestration, immobilization, and detection of these ions. Using the ion-exchange properties of these microspheres, this scheme has been extended to the detection of nonluminescent ions. The principles of these assays form the basis for operation of a portable microfluidic device for general analytical and nuclear forensics applications and indicate the manner in which the established methods of analytical chemistry, such as liquid-liquid extraction and ion-exchange chromatography, can be adapted for such miniature devices.
authors.affiliation: Chemical Sciences and Engineering Division, Argonne National Laboratory, 9700 South Cass Ave, Argonne, Illinois 60439, USA. shkrob@anl.gov
authors.forename: Ilya A
authors.initials: IA
authors.lastname: Shkrob
authors.forename: Michael D
authors.initials: MD
authors.lastname: Kaminski
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Mertz
authors.forename: Paul G
authors.initials: PG
authors.lastname: Rickert
authors.forename: Mark S
authors.initials: MS
authors.lastname: Derzon
authors.forename: Kamyar
authors.initials: K
authors.lastname: Rahimian