title: Aldo keto reductase 1B7 and prostaglandin F2alpha are regulators of adrenal endocrine functions.
abstract: Prostaglandin F(2alpha) (PGF(2alpha)), represses ovarian steroidogenesis and initiates parturition in mammals but its impact on adrenal gland is unknown. Prostaglandins biosynthesis depends on the sequential action of upstream cyclooxygenases (COX) and terminal synthases but no PGF(2alpha) synthases (PGFS) were functionally identified in mammalian cells. In vitro, the most efficient mammalian PGFS belong to aldo-keto reductase 1B (AKR1B) family. The adrenal gland is a major site of AKR1B expression in both human (AKR1B1) and mouse (AKR1B3, AKR1B7). Thus, we examined the PGF(2alpha) biosynthetic pathway and its functional impact on both cortical and medullary zones. Both compartments produced PGF(2alpha) but expressed different biosynthetic isozymes. In chromaffin cells, PGF(2alpha) secretion appeared constitutive and correlated to continuous expression of COX1 and AKR1B3. In steroidogenic cells, PGF(2alpha) secretion was stimulated by adrenocorticotropic hormone (ACTH) and correlated to ACTH-responsiveness of both COX2 and AKR1B7/B1. The pivotal role of AKR1B7 in ACTH-induced PGF(2alpha) release and functional coupling with COX2 was demonstrated using over- and down-expression in cell lines. PGF(2alpha) receptor was only detected in chromaffin cells, making medulla the primary target of PGF(2alpha) action. By comparing PGF(2alpha)-responsiveness of isolated cells and whole adrenal cultures, we demonstrated that PGF(2alpha) repressed glucocorticoid secretion by an indirect mechanism involving a decrease in catecholamine release which in turn decreased adrenal steroidogenesis. PGF(2alpha) may be regarded as a negative autocrine/paracrine regulator within a novel intra-adrenal feedback loop. The coordinated cell-specific regulation of COX2 and AKR1B7 ensures the generation of this stress-induced corticostatic signal.
authors.affiliation: CNRS, UMR6247-Genetic, Reproduction & Development, Clermont University, Aubière, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Lambert-Langlais
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Pointud
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Lefrançois-Martinez
authors.forename: Fanny
authors.initials: F
authors.lastname: Volat
authors.forename: Michèle
authors.initials: M
authors.lastname: Manin
authors.forename: François
authors.initials: F
authors.lastname: Coudoré
authors.forename: Pierre
authors.initials: P
authors.lastname: Val
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sahut-Barnola
authors.forename: Bruno
authors.initials: B
authors.lastname: Ragazzon
authors.forename: Estelle
authors.initials: E
authors.lastname: Louiset
authors.forename: Catherine
authors.initials: C
authors.lastname: Delarue
authors.forename: Hervé
authors.initials: H
authors.lastname: Lefebvre
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
authors.forename: Antoine
authors.initials: A
authors.lastname: Martinez
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000449:Aldehyde Reductase / Q000378:metabolism*; D000074408:Aldo-Keto Reductases; D000818:Animals; D001665:Binding Sites; D019439:Chromaffin Cells / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism*; D004703:Endocrine System; D015971:Gene Expression Regulation, Enzymologic; D005938:Glucocorticoids / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005938:Glucocorticoids; D015237:Dinoprost; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; C066498:Akr1b7 protein, mouse; D000449:Aldehyde Reductase; C000614209:AKR7A5 protein, mouse; D051546:Cyclooxygenase 2