title: Knockdown of Bmi-1 impairs growth and invasiveness of human gastric carcinoma cells.
abstract: The effect of B cell-specific MLV integration site-1 (Bmi-1) RNA interference (RNAi)-mediated inhibition of Bmi-1 expression on the proliferation, apoptosis, and invasiveness of human gastric carcinoma AGS cells was investigated. RT-PCR and Western blot analyses demonstrated that Bmi-1 expression and the Bmi-1 protein level were significantly decreased in Bmi-1 shRNA transfected AGS cells compared to untransfected and nonspecific shRNA transfected AGS cells. Bmi-1 RNAi-mediated inhibition of Bmi-1 expression significantly affected cell growth and invasiveness, and resulted in increased AGS cell apoptosis. This was not observed in untransfected and nonspecific shRNA transfected AGS cells. Inhibition of Bmi-1 expression in human gastric carcinoma cells affects cell proliferation and invasiveness.
authors.affiliation: Department of Gastroenterology and Hubei Provincial Center of Clinical Study for Digestive Diseases, Zhongnan Hospital, the Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan, PR China. xiaojunzhongnan@163.com
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Changsheng
authors.initials: C
authors.lastname: Deng
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000502:physiology; D006801:Humans; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D063150:Polycomb Repressive Complex 1; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D013274:Stomach Neoplasms / Q000473:pathology*; D025803:Tumor Suppressor Protein p14ARF / Q000502:physiology
chemical_list: C069077:BMI1 protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D025803:Tumor Suppressor Protein p14ARF; D063150:Polycomb Repressive Complex 1