title: Characterization of newly established bovine intestinal epithelial cell line.
abstract: Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Japan. kohtaroh.miyazawa@yale.edu
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hondo
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanaya
authors.forename: Sachi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Ikuro
authors.initials: I
authors.lastname: Takakura
authors.forename: Wataru
authors.initials: W
authors.lastname: Itani
authors.forename: Michael T
authors.initials: MT
authors.lastname: Rose
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology*; D051379:Mice