Acteoside and 6-O-acetylacteoside downregulate cell adhesion molecules induced by IL-1beta through inhibition of ERK and JNK in human vascular endothelial cells.
Acteoside, an active phenylethanoid glycoside of many medicinal plants and bitter tea, displays anti-inflammatory properties in vitro. However, it is unclear whether acteoside and similar compounds may inhibit the expression of cell adhesion molecules (CAMs), which plays a role in the pathogenesis of atherosclerosis and inflammation. Here, we found that acteoside, isoacteoside, and 6-O-acetylacteoside inhibited IL-1beta-activated expression of intercellular CAM-1 (ICAM-1) and vascular CAM-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs); the inhibitory potency was as follows: 6-O-acetylacteoside > acteoside > isoacteoside. Acteoside and 6-O-acetylacteoside also dose-dependently inhibited VCAM-1 gene promoter activity in IL-1beta-activated HUVECs. The inhibition of acteoside and 6-O-acetylacteoside on IL-1beta-activated expression of CAMs was manifested by decreased phosphorylation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). These results indicate that acteoside and 6-O-acetylacteoside may exert anti-inflammatory activities in vascular endothelium by inhibiting the expression of CAMs, primarily through decreased phosphorylation of ERK and JNK.
Chen
Chao-Hsiang
CH
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, ROC.
Song
Tuzz-Ying
TY
Liang
Yu-Chih
YC
Hu
Miao-Lin
ML
D002109:Caffeic Acids / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000235:genetics*; D004187:Disaccharides / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D007962:Leukocytes / Q000502:physiology; D010636:Phenols / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D014471:Umbilical Veins; D059808:Polyphenols
D002109:Caffeic Acids; D015815:Cell Adhesion Molecules; D004187:Disaccharides; D004791:Enzyme Inhibitors; D048049:Extracellular Signal-Regulated MAP Kinases; D005960:Glucosides; D053583:Interleukin-1beta; D048031:JNK Mitogen-Activated Protein Kinases; D010636:Phenols; C546692:6-O-acetylacteoside; C058956:acteoside; D059808:Polyphenols