New selective phosphodiesterase 4D inhibitors differently acting on long, short, and supershort isoforms.
The lack of selective inhibitors toward the long, short, or supershort phosphodiesterases (PDE4s) prevented researchers from carefully defining the connection between different enzyme isoforms, their brain localization, and their role in neurodegenerative diseases such as Alzheimer's disease (AD). In the search for new therapeutic agents for treating memory and learning disorders, we synthesized new rolipram related PDE4 inhibitors, which had some selectivity toward the long form PDE4D3. The first series was synthesized as racemate and then resolved by semipreparative HPLC on chiral supports. Herein we report the synthetic pathways to obtain compounds 1a-c, 2a-c, 3a-c, 4a-f, 5a,b, 6a,b, 7a,b, the chiral analytical study to resolve compounds 1a-c, 2a-c, 3a-c, the molecular docking study for compound 1c, and the biological results and some SAR considerations that provide some insights and hints for the structural requirements for PDE4D subtype selectivity and enzyme inhibition.
Bruno
Olga
O
Dipartimento di Scienze Farmaceutiche, University of Genoa, v.le Benedetto XV, 3-16132 Genoa, Italy. obruno@unige.it
Romussi
Alessia
A
Spallarossa
Andrea
A
Brullo
Chiara
C
Schenone
Silvia
S
Bondavalli
Francesco
F
Vanthuyne
Nicolas
N
Roussel
Christian
C
D001547:Benzaldehydes / Q000138:chemical synthesis* / Q000737:chemistry; D001665:Binding Sites; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000737:chemistry; D003517:Cyclopentanes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D009025:Morpholines / Q000138:chemical synthesis / Q000737:chemistry; D058988:Phosphodiesterase 4 Inhibitors; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry; D020889:Rolipram / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D001547:Benzaldehydes; D003517:Cyclopentanes; D007527:Isoenzymes; D009025:Morpholines; D058988:Phosphodiesterase 4 Inhibitors; D011994:Recombinant Proteins; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D020889:Rolipram