title: Application of electrolysis to stimulate microbial reductive PCE dechlorination and oxidative VC biodegradation.
abstract: A novel approach was applied to stimulate biodegradation of chloroethenes bya coupled bioelectro-process. In a flow-through column system, microbial dechlorination of tetrachloroethene to cis-dichloroethene, vinyl chloride, and ethene was stimulated by hydrogen produced by water electrolysis. Dechlorinating bacteria (Dehalococcoides spp. and Desulfitobacterium spp.) and also methanogens and homoacetogens were detected in the anaerobic column. Simultaneously, oxidative biodegradation of lower chlorinated metabolites (vinyl chloride) was stimulated by electrolytic oxygen formation in the corresponding aerobic column. The process was stable for more than 100 days and an average removal of approximately 23 micromol/d PCE and 72 micromo/d vinyl chloride was obtained with a current density of 0.05 mA/cm2. Abiotic electrochemical degradation of the contaminants was not observed. Microbial dechlorination correlated with the current densities in the applied range of 0.01-0.05 mA/cm2. The results are promising for environmental applications, since with electrolysis hydrogen and oxygen can be supplied continuously to chloroethene degrading microorganisms, and the supply rates can be easily controlled by adjusting the electric current.
authors.affiliation: Water Technology Center, Department of Environmental Biotechnology, Karlsruhe, Germany.
authors.forename: Svenja T
authors.initials: ST
authors.lastname: Lohner
authors.forename: Andreas
authors.initials: A
authors.lastname: Tiehm
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004560:Electricity; D004572:Electrolysis; D054879:Halogenation; D010084:Oxidation-Reduction; D011143:Polyvinyl Chloride / Q000378:metabolism*; D014752:Vinyl Chloride / Q000378:metabolism*
chemical_list: D011143:Polyvinyl Chloride; D014752:Vinyl Chloride