title: Beyond directed evolution: Darwinian selection as a tool for synthetic biology.
abstract: Synthetic biology is an engineering approach that seeks to design and construct new biological parts, devices and systems, as well as to re-design existing components. However, rationally designed synthetic circuits may not work as expected due to the context-dependence of biological parts. Darwinian selection, the main mechanism through which evolution works, is a major force in creating biodiversity and may be a powerful tool for synthetic biology. This article reviews selection-based techniques and proposes strict Darwinian selection as an alternative approach for the identification and characterization of parts. Additionally, a strategy for fine-tuning of relatively complex circuits by coupling them to a master standard circuit is discussed.
authors.affiliation: Fundació General de la Universitat de València, Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Postal Code 22085, 46071 València, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Porcar