Biomarkers of cellular reaction to pesticide exposure in a rural population.
In the present study we report data obtained from the evaluation of subjects occupationally exposed to pesticide mixtures from Santa Fe province, Argentina, using biomarkers for butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities, catalase (CAT), lipid peroxidation (by TBARS assay) and the Damage Index Comet Assay (DICA). Our results showed an AChE inhibition (25% and 15% in directly and indirectly groups, respectively) in relation to controls with no significant modifications in BChE. TBARS levels were higher (51%) in pesticide sprayers while CAT activity was reduced in both, applicators (61%) and non-applicators (43%). DICA was significantly increased in direct (83%) and indirect (98%) exposed groups, compared with controls. These results showed modifications in lipid peroxidation, antioxidant defence system, and DNA damage in lymphocytes of exposed workers. Further investigations are suggested in order to link our findings with adverse health effects observed in chronic pesticide toxicity, where oxidative damage plays a pathophysiological role.
Simoniello
María Fernanda
MF
Cátedra de Toxicología, Farmacología y Bioquímica Legal, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina. macarballo2003@yahoo.com.ar
Kleinsorge
Elisa Carlotta
EC
Scagnetti
Jorge Alberto
JA
Mastandrea
Carlos
C
Grigolato
Raúl Alberto
RA
Paonessa
Adriana Monica
AM
Carballo
Marta Ana
MA
D000110:Acetylcholinesterase / Q000032:analysis / Q000097:blood; D000328:Adult; D000382:Agricultural Workers' Diseases / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D001118:Argentina; D015415:Biomarkers / Q000097:blood; D002091:Butyrylcholinesterase / Q000032:analysis / Q000097:blood; D016022:Case-Control Studies; D002374:Catalase / Q000032:analysis / Q000097:blood; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000032:analysis*; D010575:Pesticides / Q000009:adverse effects*; D055815:Young Adult
D015415:Biomarkers; D010575:Pesticides; D002374:Catalase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase