title: Electrophoretic exclusion for the selective transport of small molecules.
abstract: A novel method capable of differentiating and concentrating small molecules in bulk solution termed "electrophoretic exclusion" is described and experimentally investigated. In this technique, the hydrodynamic flow of the system is countered by the electrophoretic velocity to prevent a species from entering into the channel. The separation can be controlled by changing the flow rate or applied electric field in order to exclude certain species selectively while allowing others to pass through the capillary. Proof of principle studies employed a flow injection regime of the method and examined the exclusion of Methyl Violet dye in the presence of a neutral species. Methyl Violet was concentrated almost 40 times the background concentration in 30 s using 6 kV. Additionally, a threshold voltage necessary for exclusion was determined. The establishment of a threshold voltage enabled the differentiation of two similar cationic species: Methyl Green and Neutral Red.
authors.affiliation: Arizona State University, Tempe, AZ 85287, USA.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Meighan
authors.forename: Michael W
authors.initials: MW
authors.lastname: Keebaugh
authors.forename: Alicia M
authors.initials: AM
authors.lastname: Quihuis
authors.forename: Stacy M
authors.initials: SM
authors.lastname: Kenyon
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hayes
mesh_terms: D004574:Electromagnetic Fields; D019075:Electrophoresis, Capillary / Q000379:methods*; D005840:Gentian Violet / Q000737:chemistry; D007526:Isoelectric Point; D008739:Methyl Green / Q000737:chemistry; D008970:Molecular Weight; D009499:Neutral Red / Q000737:chemistry
chemical_list: D009499:Neutral Red; D008739:Methyl Green; D005840:Gentian Violet