title: Label-free and time-resolved measurements of cell volume changes by surface plasmon resonance (SPR) spectroscopy.
abstract: Cell volume and its regulation is one of the key players for cellular integrity and a strong indicator for several cell pathologies. But time-resolved volume measurements of adherently grown mammalian cells using established methods, such as extracellular impedance analysis or light microscopy, are complex and time-consuming. In this study, we demonstrate that surface plasmon resonance spectroscopy (SPR) is a powerful transducer device capable of reporting volume changes of cells that are directly grown on the SPR sensor surface. The approach is label-free, non-invasive and provides an outstanding time resolution. In proof-of-principle studies we recorded the volume change of confluent MDCK II cells induced by hypo- or hypertonic stimulation in a time-resolved manner. Comparison of the SPR-based experiments reported here with more recent studies using different approaches suggests a direct correlation between SPR signal shift and cell volume changes.
authors.affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany. rudolf.robelek@chemie.uni-regensburg.de
authors.forename: R
authors.initials: R
authors.lastname: Robelek
authors.forename: J
authors.initials: J
authors.lastname: Wegener
mesh_terms: D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D048429:Cell Size; D004285:Dogs; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007668:Kidney / Q000166:cytology* / Q000502:physiology*; D012031:Refractometry / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*