OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice.
The rice Xa21 gene, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), encodes a receptor-like kinase. Few components involved in transducing the Xa21-mediated defense response have yet been identified. Here, we report that XA21 binds to a WRKY transcription factor, called OsWRKY62. The OsWRKY62 gene encodes two splice variants (OsWRKY62.1 and OsWRKY62.2). OsWRKY62.1:smGFP2 and OsWRKY62.2:smGFP2 fusion proteins partially localize to the nucleus. Transgenic plants overexpressing OsWRKY62.1 are compromised in basal defense and Xa21-mediated resistance to Xoo. Furthermore, overexpression of OsWRKY62.1 suppresses the activation of defense-related genes. These results imply that OsWRKY62 functions as a negative regulator of innate immunity in rice, and serves as a critical mediator of both basal and race-specific defense responses.
Peng
Ying
Y
Department of Plant Pathology, University of California, Davis, CA 95616, USA.
Bartley
Laura E
LE
Chen
Xuewei
X
Dardick
Christopher
C
Chern
Mawsheng
M
Ruan
Randy
R
Canlas
Patrick E
PE
Ronald
Pamela C
PC
D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control*; D018515:Plant Leaves / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014974:Xanthomonas
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors