Simultaneous determination of tegafur and gimeracil in human plasma by liquid chromatography/tandem mass spectrometry.
We developed a rapid, simple and sensitive LC/MS/MS method for the simultaneous quantitation of tegafur (FT) and gimeracil (CDHP) in human plasma with a concentration range of 20-5000 and 2-500 ng/mL, respectively. Methanol was chosen as a precipitation agent for sample preparation. Chromatographic separation was performed on an inertsil ODS-3 C18 column using 1.0% formic acid in water and methanol (80/20, v/v) at a flow rate of 0.3 mL/min. The MS detection was operated with selected reaction monitoring (SRM) in the positive-ion mode. The matrix effect ranged from -8.9 to 7.8% for all analytes. The intra- and inter-day precisions were less than 8.6 and 9.5%, and the accuracy was within +/-7.5% for all analytes, respectively. The mean recoveries were 76.5 +/- 5.2 and 78.3 +/- 5.9% for FT and CDHP, respectively. The analytes were stable under all possible conditions of storing and handling for each compound.
Gu
Yuan
Y
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Lu
Rong
R
Si
Duanyun
D
Liu
Changxiao
C
D053000:Analytic Sample Preparation Methods; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002853:Chromatography, Liquid; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D011725:Pyridines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D005641:Tegafur / Q000097:blood*; D013997:Time Factors
D011725:Pyridines; D005641:Tegafur; C104201:gimeracil