title: Highly sensitive protein detection based on lanthanide chelates with antenna ligands providing a linear range of five orders of magnitude.
abstract: Protein detection is an important task for pharmaceutical and clinical research today. Numerous protein staining techniques exist but are limited regarding their sensitivity and often narrow linear quantification ranges. To the best of our knowledge, this is the first description of a novel class of lanthanide chelatators, which absorb in the lower energy region at 360 nm. The new compound (6,9-dicarboxymethyl-3-{4-([1,10]-phenanthrol-2-ylethinylphenyl-carbamoyl)-methyl}-3,6,9-triaza-)-undeca-1,11-dicarboxylic acid) was coupled to different proteins and showed highly sensitive protein detection limits in both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (1.5 fmol of bovine serum albumin) as well as Dot Blot (100 amol of lysozyme). Furthermore, the novel compound shows an exceptionally broad linear quantification range over 5 orders of magnitude allowing applications that require the highest sensitivity alongside standard sensitivity. In addition, the new compound offers multiplexing capabilities.
authors.affiliation: Institute of Bioanalytical Chemistry, Center of Biotechnology and Biomedicine, Faculty of Chemistry and Mineralogy, Leipzig University, Deutscher Platz 5, 04103 Leipzig, Germany. zuechner@rz.uni-leipzig.de
authors.forename: Thole
authors.initials: T
authors.lastname: Zuchner
authors.forename: Frank
authors.initials: F
authors.lastname: Schumer
authors.forename: Renate
authors.initials: R
authors.lastname: Berger-Hoffmann
authors.forename: Katrin
authors.initials: K
authors.lastname: Müller
authors.forename: Mathias
authors.initials: M
authors.lastname: Lukas
authors.forename: Kornelia
authors.initials: K
authors.lastname: Zeckert
authors.forename: Jörg
authors.initials: J
authors.lastname: Marx
authors.forename: Horst
authors.initials: H
authors.lastname: Hennig
authors.forename: Ralf
authors.initials: R
authors.lastname: Hoffmann
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D011506:Proteins