LuMPIS--a modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs.
The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems.
Vizoso Pinto
Maria Guadalupe
MG
Max von Pettenkofer-Institute, Ludwig-Maximilians University, Munich, Germany.
Villegas
Josefina Maria
JM
Peter
Jan
J
Haase
Rudolf
R
Haas
Jürgen
J
Lotz
Amelie Sophia
AS
Muntau
Ania Carolina
AC
Baiker
Armin
A
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D020862:GC Rich Sequence; D015967:Gene Expression Regulation, Viral; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D058619:Maltose-Binding Proteins; D016366:Open Reading Frames; D025941:Protein Interaction Mapping / Q000191:economics / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D049490:Systems Biology / Q000379:methods; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D014764:Viral Proteins