Selective formation of a linear-shaped bundle of microtubules.
By using rigid microtubules (MTs) prepared by polymerization with guanylyl-(alpha, beta)-methylene-diphosphonate GMPCPP, giant straight-shaped MT bundles were selectively obtained through a dynamic self-assembly process. We demonstrate the effect of the rigidity on the shape and motility of MT bundle composed of GMPCPP-polymerized MTs (GMPCPP-MTs) compared with control MTs that were polymerized with GTP and stabilized with paclitaxel.
Kawamura
Ryuzo
R
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
Kakugo
Akira
A
Osada
Yoshihito
Y
Gong
Jian Ping
JP
D000818:Animals; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism*; D009068:Movement; D013552:Swine; D014404:Tubulin / Q000378:metabolism
D014404:Tubulin; C004805:5'-guanylylmethylenebisphosphonate; D006160:Guanosine Triphosphate; D016547:Kinesins