title: Maternal vitamin D deficiency leads to cardiac hypertrophy in rat offspring.
abstract: The aim of this study was to determine the effect of vitamin D deficiency from conception until 4 weeks of age on the development of the heart in rat offspring. Sprague-Dawley (SD) rats were fed either a vitamin D deplete or vitamin D-replete diet for 6 weeks prior to pregnancy, during pregnancy and throughout lactation. Cardiomyocyte number was determined in fixed hearts of offspring at postnatal day 3 and 4 weeks of age using an optical disector/fractionator stereological technique. In other litters, cardiomyocytes were isolated from freshly excised hearts to determine the proportion of mononucleated and binucleated cardiomyocytes. Maternal vitamin D deficiency had no effect on cardiomyocyte number, cardiomyocyte area, or the proportion of mononucleated/binucleated cardiomyocytes in 3-day-old male and female offspring. Importantly, however, vitamin D deficiency led to an increase in left ventricle (LV) volume that was accompanied by an increase in cardiomyocyte number and size, and in the proportion of mononucleated cardiomyocytes at 4 weeks of age. Our findings suggest that exposure to vitamin D deficiency in utero and early life leads to delayed maturation and subsequent enhanced growth (proliferation and hypertrophy) of cardiomyocytes in the LV. This may lead to altered cardiac function later in life.
authors.affiliation: Department of Anatomy & Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Oksan
authors.initials: O
authors.lastname: Gezmish
authors.forename: Marianne
authors.initials: M
authors.lastname: Tare
authors.forename: Helena C
authors.initials: HC
authors.lastname: Parkington
authors.forename: Ruth
authors.initials: R
authors.lastname: Morley
authors.forename: Enzo R
authors.initials: ER
authors.lastname: Porrello
authors.forename: Kristen J
authors.initials: KJ
authors.lastname: Bubb
authors.forename: Mary Jane
authors.initials: MJ
authors.lastname: Black
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D002112:Calcifediol / Q000097:blood; D002118:Calcium / Q000097:blood; D006332:Cardiomegaly / Q000209:etiology* / Q000473:pathology*; D002452:Cell Count; D002455:Cell Division; D002467:Cell Nucleus / Q000473:pathology; D005260:Female; D008297:Male; D032383:Myocytes, Cardiac / Q000473:pathology; D009929:Organ Size; D011247:Pregnancy; D011248:Pregnancy Complications / Q000473:pathology*; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014807:Vitamin D / Q000494:pharmacology; D014808:Vitamin D Deficiency / Q000150:complications*
chemical_list: D014807:Vitamin D; D002112:Calcifediol; D002118:Calcium