title: [TLR4 signals are involved in multiple myeloma cell proliferation and apoptosis].
abstract: To investigate the TLR4 signaling in multiple myeloma cell proliferation and apoptosis.
  
  
  
  TLR4 gene transcription and protein expression were detected by RT-PCR and PE flow cytometry staining; the cells proliferation were detected by MTT assay; doxorubicin-induced apoptosis of cells was detected by AnnexinV-PI double staining flow cytometry.
  
  
  
  TLR4 gene transcription and protein expression was detected in the myeloma cell lines. Under the lipopolysaccharides (LPS) stimulation, MM1-s cells TLR4 positive proliferation significantly increased (P<0.05), but not found in U266 cells TLR4 negative; MM1-s cells showed the resistance to doxorubisin-induced apoptosis, but LPS did not protect doxorubicin-induced U266 cell apoptosis.
  
  
  
  TLR4 signaling may play an important role both in multiple myeloma proliferation and survival.
authors.affiliation: The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Han-Ying
authors.initials: HY
authors.lastname: Bao
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cai
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4