title: Optical fiber biosensor coupled to chromatographic separation for screening of dopamine, norepinephrine and epinephrine in human urine and plasma.
abstract: An optical fiber biosensor has been developed for the determination of catecholamines (dopamine, norepinephrine and epinephrine) based on the recognition capacity of the enzyme laccase. In this study, a glass tube constituted by a fused silica fiber coated with a film of polystyrene/divinylbenzene resin (PS/DVB) was used for catecholamines separation. Firstly, the analyzer was tested for calibration and its analytical performance for catecholamines detection was compared with a classical analytical method, namely high performance liquid chromatography-electrochemical detector (HPLC-ED). The developed analytical device shows a high potential for catecholamines quantification with a detection limit of 2.1, 2.6 and 3.4 pg mL(-1) for dopamine, norepinephrine and epinephrine, respectively. The analytical sensitivity, inferred from the slope of the calibration curves established for a range of concentrations between 5 and 125 pg mL(-1), was found to be 0.344, 0.252 and 0.140 dB/pg mL(-1) for dopamine, norepinephrine and epinephrine, respectively. Furthermore, catecholamines speciation with the PS/DVB fiber was completely achieved in 3 min. The analytical performance of the reported sensor was also evaluated and found adequate for catecholamines determination in human urine and plasma samples.
authors.affiliation: ISEIT/Viseu-Instituto Piaget, Estrada do Alto do Gaio, Galifonge, Lordosa, Viseu, Portugal. lsilva@viseu.ipiaget.org
authors.forename: Lurdes I B
authors.initials: LI
authors.lastname: Silva
authors.forename: Filipe D P
authors.initials: FD
authors.lastname: Ferreira
authors.forename: Ana C
authors.initials: AC
authors.lastname: Freitas
authors.forename: Teresa A P
authors.initials: TA
authors.lastname: Rocha-Santos
authors.forename: A C
authors.initials: AC
authors.lastname: Duarte
mesh_terms: D000704:Analysis of Variance; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D004563:Electrochemistry; D004837:Epinephrine / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D009638:Norepinephrine / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D055100:Optical Fibers; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D011137:Polystyrenes; C432006:divinylbenzene-polystyrene copolymer; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine