title: DNA photocleavage activity of cobalt(III) polypyridyl complexes containing dpq ligand.
abstract: Four cobalt(III) polypyridyl complexes, [Co(phen)(3-)(n)(dpq)(n)](3+) (phen=1,10-phenanthroline, dpq=dipyrido[3,2-f:2',3'-h]-quinoxaline) (n=0, 1, 2, and 3) were synthesized and the influences of the dpq ligand on the photophysical properties, electrochemical properties, DNA binding affinities, as well as photonuclease activities of the complexes, were examined in detail. The presence of dpq ligand increases the DNA binding affinities of the corresponding complexes remarkably with respect to [Co(phen)(3)](3+). With the sequential substitution of phen ligand by dpq ligand, the (1)O(2) quantum yields of the corresponding complexes are enhanced greatly. As a result, the photonuclease activities follow the order of [Co(dpq)(3)](3+)>[Co(phen)(dpq)(2)](3+)>[Co(phen)(2)(dpq)](3+)>>[Co(phen)(3)](3+). It was found all the examined complexes can generate ()OH upon UV irradiation, and ()OH is also involved in DNA photocleavage as reactive oxygen species.
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Qian-Xiong
authors.initials: QX
authors.lastname: Zhou
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Chen
authors.forename: Yuan-Jun
authors.initials: YJ
authors.lastname: Hou
authors.forename: Wan-Hua
authors.initials: WH
authors.lastname: Lei
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Wang
authors.forename: Bao-Wen
authors.initials: BW
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D003035:Cobalt / Q000737:chemistry*; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D053837:DNA Cleavage; D003851:Deoxyribonucleases / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008024:Ligands; D010618:Phenanthrolines / Q000737:chemistry; D010782:Photolysis
chemical_list: D056831:Coordination Complexes; D008024:Ligands; D010618:Phenanthrolines; D003035:Cobalt; D004247:DNA; C427203:calf thymus DNA; D003851:Deoxyribonucleases