UV-defined flat PDMS stamps suitable for microcontact printing.
We report a simple method of creating well-defined micropatterns on the surface of a flat PDMS stamp, making it suitable for microcontact printing of proteins. This method only requires a UV lamp (254 nm) and a TEM grid (as a photomask) to modify the surface of PDMS for creating desired micropatterns. By using the UV-modified stamp, a printed protein micropattern that resembles the original TEM grid can be obtained. Surprisingly, unlike the oxygen-plasma-treated PDMS, the UV-modified flat stamp is also long-lasting (>1 week). The method reported herein is very economical for microcontact printing applications because expensive silicon masters and microstructured PDMS are no longer required.
Xue
Chang-Ying
CY
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576.
Chin
Shi Yao
SY
Khan
Saif A
SA
Yang
Kun-Lin
KL
D000818:Animals; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011327:Printing; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013997:Time Factors; D014466:Ultraviolet Rays
D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; C013830:baysilon