Global analysis of gene expression profiles in Brassica napus developing seeds reveals a conserved lipid metabolism regulation with Arabidopsis thaliana.
In order to study Brassica napus fatty acid (FA) metabolism and relevant regulatory networks, a systematic identification of fatty acid (FA) biosynthesis-related genes was conducted. Following gene identification, gene expression profiles during B. napus seed development and FA metabolism were performed by cDNA chip hybridization (>8000 EST clones from seed). The results showed that FA biosynthesis and regulation, and carbon flux, were conserved between B. napus and Arabidopsis. However, a more critical role of starch metabolism was detected for B. napus seed FA metabolism and storage-component accumulation when compared with Arabidopsis. In addition, a crucial stage for the transition of seed-to-sink tissue was 17-21 d after flowering (DAF), whereas FA biosynthesis-related genes were highly expressed primarily at 21 DAF. Hormone (auxin and jasmonate) signaling is found to be important for FA metabolism. This study helps to reveal the global regulatory network of FA metabolism in developing B. napus seeds.
Niu
Ya
Y
National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science (SIBS), Chinese Academy of Sciences, 300 Fenglin Road, 200032 Shanghai, China.
Wu
Guo-Zhang
GZ
Ye
Rui
R
Lin
Wen-Hui
WH
Shi
Qiu-Ming
QM
Xue
Liang-Jiao
LJ
Xu
Xiao-Dong
XD
Li
Yao
Y
Du
Yu-Guang
YG
Xue
Hong-Wei
HW
D017360:Arabidopsis / Q000235:genetics*; D029688:Brassica napus / Q000235:genetics*; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005227:Fatty Acids / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D050356:Lipid Metabolism / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics*
D018076:DNA, Complementary; D005227:Fatty Acids