Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.
A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.
Carroll
James
J
Department of Biological Science, Cork Institute of Technology, Cork, Ireland.
Douarre
Pierre
P
Coffey
Aidan
A
Buckley
Jim
J
Cashman
Bill
B
O'Farrell
Kevin
K
O'Mahony
Jim
J
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D009162:Mycobacterium avium / Q000166:cytology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology