title: Extraction and mechanism investigation of trace roxithromycin in real water samples by use of ionic liquid-salt aqueous two-phase system.
abstract: The ionic liquid, as a green solvent, has several advantages over the organic solvents in traditional liquid-liquid extraction. Aqueous two-phase system (ATPS) consisting of a hydrophilic ionic liquid (1-butyl-3-methylimidazolium tetrafluoraborate, [Bmim]BF(4)) and Na(2)CO(3), which is a novel, simple, non-toxic and effective sample pretreatment technique coupled with molecular fluorescence spectrophotometry, was developed for the simultaneous separation, enrichment and rapid analysis of roxithromycin. The extraction yield of roxithromycin in [Bmim]BF(4)-Na(2)CO(3) aqueous two-phase system is influenced by the types of salts, concentrations of Na(2)CO(3) and [Bmim]BF(4), as well as the extracting temperature. Under the optimum conditions, the average extraction efficiency is up to 90.7%. The mechanism of ionic liquid-salt ATPS formation was discussed by hydration theory, and the extraction mechanism of the [Bmim]BF(4)-salt ATPS was investigated by FT-IR spectroscopy and UV-vis spectroscopy. The results demonstrate that no chemical (bonding) interactions are observed between ionic liquid and roxithromycin, while the nature properties of the roxithromycin are not altered. This method was practical when applied to the analysis of roxithromycin in real water samples with the detection limit of 0.03 microg mL(-1), relative standard deviation (RSD) of 1.9% (n=13), and linear ranges of 1.00-20.00 microg mL(-1). The proposed extraction technique will be promising in the separation of other small biomolecules.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Yan
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Pan
mesh_terms: D002254:Carbonates / Q000737:chemistry*; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D015575:Roxithromycin / Q000032:analysis*; D012492:Salts / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D002254:Carbonates; D007093:Imidazoles; D052578:Ionic Liquids; D012492:Salts; D014867:Water; D015575:Roxithromycin; C005686:sodium carbonate