title: Reverse breeding: a novel breeding approach based on engineered meiosis.
abstract: Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on reducing genetic recombination in the selected heterozygote by eliminating meiotic crossing over. Male or female spores obtained from such plants contain combinations of non-recombinant parental chromosomes which can be cultured in vitro to generate homozygous doubled haploid plants (DHs). From these DHs, complementary parents can be selected and used to reconstitute the heterozygote in perpetuity. Since the fixation of unknown heterozygous genotypes is impossible in traditional plant breeding, RB could fundamentally change future plant breeding. In this review, we discuss various other applications of RB, including breeding per chromosome.
authors.affiliation: Rijk Zwaan Breeding BV, Fijnaart, The Netherlands.
authors.forename: Rob
authors.initials: R
authors.lastname: Dirks
authors.forename: Kees
authors.initials: K
authors.lastname: van Dun
authors.forename: C Bastiaan
authors.initials: CB
authors.lastname: de Snoo
authors.forename: Mark
authors.initials: M
authors.lastname: van den Berg
authors.forename: Cilia L C
authors.initials: CL
authors.lastname: Lelivelt
authors.forename: William
authors.initials: W
authors.lastname: Voermans
authors.forename: Leo
authors.initials: L
authors.lastname: Woudenberg
authors.forename: Jack P C
authors.initials: JP
authors.lastname: de Wit
authors.forename: Kees
authors.initials: K
authors.lastname: Reinink
authors.forename: Johan W
authors.initials: JW
authors.lastname: Schut
authors.forename: Eveline
authors.initials: E
authors.lastname: van der Zeeuw
authors.forename: Aat
authors.initials: A
authors.lastname: Vogelaar
authors.forename: Gerald
authors.initials: G
authors.lastname: Freymark
authors.forename: Evert W
authors.initials: EW
authors.lastname: Gutteling
authors.forename: Marina N
authors.initials: MN
authors.lastname: Keppel
authors.forename: Paul
authors.initials: P
authors.lastname: van Drongelen
authors.forename: Matthieu
authors.initials: M
authors.lastname: Kieny
authors.forename: Philippe
authors.initials: P
authors.lastname: Ellul
authors.forename: Alisher
authors.initials: A
authors.lastname: Touraev
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Hans
authors.initials: H
authors.lastname: de Jong
authors.forename: Erik
authors.initials: E
authors.lastname: Wijnker
mesh_terms: D001947:Breeding / Q000379:methods*; D018556:Crops, Agricultural / Q000235:genetics / Q000254:growth & development; D003434:Crossing Over, Genetic; D055785:Gene Knockdown Techniques; D018745:Genome, Plant; D006579:Heterozygote; D008540:Meiosis; D063245:Plant Development; D010944:Plants / Q000235:genetics