Rabies glycoprotein fused with B subunit of cholera toxin expressed in tobacco plants folds into biologically active pentameric protein.
The pentameric B subunit of cholera toxin (CtxB) is an efficient mucosal adjuvant for vaccines. We report the expression of a chimeric protein comprising the synthetic cholera toxin B subunit fused at its C-terminal with rabies surface glycoprotein (G protein) in tobacco plants. The approximately 80.3 kDa fusion polypeptide expressed at 0.4% of the total soluble protein in leaves of the selected transgenic lines. The fusion protein formed a approximately 403 kDa pentameric protein which was functionally active in binding to GM1 receptor. The plant-made protein had a higher affinity for GM1 receptor than the native bacterial CtxB. The pentameric fusion protein was recognized by the anti-cholera toxin as well as anti-rabies antibodies. Its immuno-protective ability against rabies remains to be examined.
Roy
Sribash
S
National Botanical Research Institute, Council for Scientific and Industrial Research, Rana Pratap Marg, UP, Lucknow 226001, India.
Tyagi
Antariksh
A
Tiwari
Siddharth
S
Singh
Ankit
A
Sawant
Samir V
SV
Singh
Pradhyumna K
PK
Tuli
Rakesh
R
D000956:Antigens, Viral / Q000235:genetics*; D002772:Cholera Toxin / Q000235:genetics*; D022121:Cholera Vaccines / Q000276:immunology; D006023:Glycoproteins / Q000235:genetics*; D017510:Protein Folding; D055503:Protein Multimerization; D011819:Rabies Vaccines / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics*
D000956:Antigens, Viral; D022121:Cholera Vaccines; D006023:Glycoproteins; D011819:Rabies Vaccines; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; D002772:Cholera Toxin