title: Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera beta-glucosidase genes.
abstract: Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and beta-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an alpha-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and beta-glucosidase was able to produce ethanol from beta-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.
authors.affiliation: School of Life Science and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: Eugene
authors.initials: E
authors.lastname: Jeon
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Hyeon
authors.forename: Dong Jin
authors.initials: DJ
authors.lastname: Suh
authors.forename: Young-Woong
authors.initials: YW
authors.lastname: Suh
authors.forename: Seoung Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Song
authors.forename: Sung Ok
authors.initials: SO
authors.lastname: Han
mesh_terms: D002480:Cellulase / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism*; D048013:Acetivibrio thermocellus / Q000201:enzymology*; D000431:Ethanol / Q000138:chemical synthesis*; D005947:Glucose / Q000378:metabolism; D030781:Organisms, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012442:Saccharomycopsis / Q000201:enzymology*; D001617:beta-Glucosidase / Q000235:genetics*
chemical_list: D000431:Ethanol; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose