title: Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum.
abstract: In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle. The 70% decreased activity of isocitrate dehydrogenase, achieved by start codon exchange, resulted in a >40% improved lysine production. By flux analysis, this could be correlated to a flux shift from the TCA cycle toward anaplerotic carboxylation.
authors.affiliation: Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaussstr. 17, D-38106 Braunschweig, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Becker
authors.forename: Corinna
authors.initials: C
authors.lastname: Klopprogge
authors.forename: Hartwig
authors.initials: H
authors.lastname: Schröder
authors.forename: Christoph
authors.initials: C
authors.lastname: Wittmann
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002952:Citric Acid Cycle; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005818:Genetic Engineering; D008239:Lysine / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008239:Lysine