title: DDAH2 mRNA expression is inversely associated with some cardiovascular risk-related features in healthy young adults.
abstract: The purpose of this study was to evaluate whether the mRNA expression profiles of three genes (PRMT1, DDAH2 and NOS3) are related to ADMA metabolism and signalling, and the potential relationships with anthropometrical, biochemical, lifestyle and inflammatory indicators in healthy young adults. An emphasis on the putative effect of different mRNA expression on cardiovascular risk-related features was paid. Anthropometrical measurements as well as lifestyle features were analyzed in 120 healthy young adults. Fasting blood samples were collected for the measurement of glucose and lipid profiles as well as the concentrations of selected inflammatory markers. Profiles of mRNA expression were assessed for PRMT1, DDAH2 and NOS3 genes from peripheral blood mononuclear cells. Regarding inflammatory biomarkers, DDAH2 was inversely associated with IL-6 and TNF-alpha. Moreover, subjects in the highest quintile of DDAH2 mRNA expression showed a reduced risk to have higher values of waist circumference, and to be more prone to show higher values of HDL-c. Interestingly, DDAH2 gene expression seemed to be related with some anthropometrical, biochemical, lifestyle and inflammatory indicators linked to cardiovascular risk in apparently healthy young adults, emerging as a potential disease marker.
authors.affiliation: Department of Nutrition and Food Science, Physiology and Toxicology, University of Navarra, Pamplona, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Puchau
authors.forename: Helen Hermana M
authors.initials: HH
authors.lastname: Hermsdorff
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Zulet
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martínez
mesh_terms: D000328:Adult; D000581:Amidohydrolases / Q000097:blood / Q000235:genetics*; D000886:Anthropometry; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008019:Life Style; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000097:blood / Q000235:genetics*; D011484:Protein-Arginine N-Methyltransferases / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D012097:Repressor Proteins / Q000097:blood / Q000235:genetics*; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D012333:RNA, Messenger; D012097:Repressor Proteins; D014409:Tumor Necrosis Factor-alpha; C487735:dimethylarginine; D001120:Arginine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases; D000581:Amidohydrolases; C059999:dimethylargininase