Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies.
OBJECTIVE
The aim of this study was to investigate the levels of cytokines in placenta-derived mesenchymal stem cells (MSCs) in normal pregnancies and those with pre-eclampsia.


METHODS
C5a, CD40 Ligand, G-CSF, GM-CSF, GROalpha, I-309, sICAM-1, IFN-gamma, IL-1alpha, IL-1beta, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-16, IL-17, IL-17E, IL-23, IL-27, IL-32alpha, IP-10, I-TAC, MCP-1, MIF, MIP-1alpha, MIP-1beta, Serpin E1, RANTES, SDF-1, TNFalpha, and sTREM-1 were measured in mesenchymal stem cells using the human cytokine array panel A. The soluble intracellular adhesion molecule-1 (sICAM-1), stromal-derived factor-1 (SDF-1) and monocyte chemotactic protein-1 (MCP-1) were measured by real-time PCR and confirmed by Western blot analysis.


RESULTS
MSCs derived from the deciduas of normal pregnancies had significantly elevated levels of sICAM (p=0.000) and SDF-1 (p=0.011), compared to the pregnancies with pre-eclampsia. The level of MCP-1 in the decidua-derived MSCs was not significantly different. No significant difference was observed between normal and pre-eclamptic pregnancies for the amnion-derived MSCs.


CONCLUSIONS
The decreased levels of sICAM and SDF-1 found in the decidua-derived MSCs from pre-eclamptic pregnancies might be associated with some of the immunological alterations in pre-eclampsia.
Hwang
Jong Ha
JH
Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Republic of Korea.
Lee
Mi Jung
MJ
Seok
Oye Sun
OS
Paek
Yu Chin
YC
Cho
Geum Joon
GJ
Seol
Hyun Joo
HJ
Lee
Jae Kwan
JK
Oh
Min Jeong
MJ
D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006801:Humans; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D010920:Placenta / Q000166:cytology* / Q000276:immunology; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy / Q000276:immunology*
D018932:Chemokine CCL2; D054377:Chemokine CXCL12; D016207:Cytokines; D018799:Intercellular Adhesion Molecule-1