Oxygen recovery up-regulates avian UCP and ANT in newly hatched ducklings.
At hatching, breaking eggshell induces a surge in oxygen availability that is likely to generate oxidative stress in newborn chicks. To investigate the involvement of potential adaptive antioxidant mechanisms, we explored some markers of oxidative stress and the regulation of muscle avian uncoupling protein (avUCP) and adenine nucleotide translocase (ANT) in ducklings in the peri-hatching period. When compared with pre-hatching levels, the amount of peroxidized lipids were increased 24 h after external pipping in gastrocnemius muscle (+37%) and heart (+39%) as well as the muscle avUCP mRNA expression (+60%) but the susceptibility of red blood cells to free radicals (a functional test of oxidative status) was not affected. In order to relate these changes to the oxidative transition of hatching, an imposed hypoxia/re-oxygenation protocol was used. Hatched chicks that had spent the last 24 h of incubation in artificial severe hypoxia showed a rise in muscle (+50%) and heart (+69%) lipid peroxidation, an increased susceptibility of red blood cells to free radicals, a marked over-expression of avUCP mRNA (+105%) and a rise in mitochondrial ANT content (+54%). These results suggest that avian UCP and ANT may contribute to prepare incubating eggs to the oxidative stress generated by the hypoxia/re-oxygenation transition naturally occurring at hatching.
Rey
Benjamin
B
Laboratoire de Physiologie Intégrative, Cellulaire et Moléculaire, CNRS, Université Lyon 1, Université de Lyon, 43 Bvd 11 Novembre 1918, 69622, Villeurbanne Cedex, France. benjamin.rey@univ-lyon1.fr
Spée
Marion
M
Belouze
Maud
M
Girard
Aurélie
A
Prost
Josiane
J
Roussel
Damien
D
Duchamp
Claude
C
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D030161:Avian Proteins / Q000378:metabolism*; D004372:Ducks / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D000071245:Mitochondrial Uncoupling Proteins; D009132:Muscles / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010100:Oxygen / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D030161:Avian Proteins; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C422907:mitochondrial uncoupling protein, chicken; D000226:Mitochondrial ADP, ATP Translocases; D010100:Oxygen