[The effects and mechanisms of Forsythia suspense on the expression of Foxp3 on splenocytes and level of Treg in peripheral blood in severely burnt rats].
OBJECTIVE
To explore the immunity modulation function of aqueous of Forsythia suspense (AFS) and its possible mechanisms.


METHODS
Rats of burned model group were burned with vapor under 3 mpa pressure and 108 degrees C temperature for 8 seconds to achieve deep partial-thickness burn, to make a thirty percent total body surface area (TBSA) burn. The experiment were divided into five groups:


METHODS
without any treatment; 8PBH group: 8 h after burn; the rats of AFS1 group, AFS2 group and AFS3 group of them were given AFS 5 g/kg, 2.5 g/kg, 1.25 g/kg once a day by Po. pathway for seven days before burns, respectively. Rats were sacrificed before and 8h after burn, The percentage of Treg cells in CD4(+) T cells was detected by flow cytometry (FCM); the expression of Foxp3 mRNA on splenocytes were measured by RT-PCR, and the protein of Foxp3 activity was evaluated by immunohistochemistry staining.


RESULTS
Compared with CONTROL GROUP, the expression of Foxp3 mRNA and protein on the splenocytes were upregulated markedly (P<0.01), and the percentage of Treg were significantly increased (P<0.01) in the 8PBH group. AFS1, AFS2 and AFS3 significantly attenuated these increases (P<0.01), which was dose-dependent.


CONCLUSIONS
AFS has immunity modulation function and mechanism of it is corrected with Foxp3 mRNA on splenocytes.
Fu
Ying-Jun
YJ
Department of Pharmacology, Nanchang University Medical College, Nanchang 330006, China. fuyingjun123@126.com
Yuan
Juan-Li
JL
Chen
Jiang
J
Xie
Yong
Y
D000818:Animals; D002056:Burns / Q000097:blood / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D031657:Forsythia / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013535:Suspensions; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D004365:Drugs, Chinese Herbal; D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat; D053645:Interleukin-2 Receptor alpha Subunit; D012333:RNA, Messenger; D013535:Suspensions