title: Interaction of sulfur mustard with rat liver salt fractionated chromatin.
abstract: In this study, the interaction of an alkylating agent, sulfur mustard (SM) with rat liver active (S1 and S2) and inactive (P2) chromatin was investigated employing UV/vis spectroscopy and gel electrophoreses. The results show that SM affects the chromatin structure in a dose-dependent manner. The binding of SM to fractions is different. At lower concentrations (<500 microM), SM seems to unfold the structure and at higher concentrations, it induces aggregation and condensation of chromatin possibly via forming cross-links between the chromatin components. The extent of condensation in S2 is higher when compared to the P2 fraction.
authors.affiliation: Department of Biochemistry-Chemical Injuries Research Center, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. drm_jafari@yahoo.com
authors.forename: Mahvash
authors.initials: M
authors.lastname: Jafari
authors.forename: M
authors.initials: M
authors.lastname: Nateghi
authors.forename: A
authors.initials: A
authors.lastname: Rabbani
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism*; D009151:Mustard Gas / Q000378:metabolism*; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D002843:Chromatin; D012965:Sodium Chloride; D004247:DNA; D009151:Mustard Gas