A general electrochemical method for label-free screening of protein-small molecule interactions.
Here we report a versatile method by which the interaction between a protein and a small molecule, and the disruption of that interaction by competition with other small molecules, can be monitored electrochemically directly in complex sample matrices.
Cash
Kevin J
KJ
Department of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Ricci
Francesco
F
Plaxco
Kevin W
KW
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000032:analysis* / Q000378:metabolism
D011506:Proteins; D054852:Small Molecule Libraries; D004247:DNA