Proteomic analysis reveals differentially expressed proteins in the rat frontal cortex after methamphetamine treatment.
Methamphetamine (MA) is an addictive psycho-stimulant and the illicit use of the drug is escalating. In the present study, we examined protein expression profiles in the rat frontal cortex exposed to a total of eight MA injections (1 mg/kg, intraperitoneal) using 2-DE based proteomics. We investigated protein changes occurring in both the cytosolic fraction and the membrane fraction. 2-DE analysis resulted in 62 cytosolic and 44 membrane protein spots that were differentially regulated in the frontal cortex of rats exposed to MA when compared to control animals. Of these spots, 47 cytosolic and 42 membrane proteins were identified respectively, using ESI-Quad-TOF, which included ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), beta-synuclein, 78 kDa glucose-regulated protein (GRP 78), gamma-enolase, dihydropyrimidase-related protein 2 (DRP 2), complexin 2 and synapsin II. These proteins are associated with protein degradation, redox regulation, energy metabolism, cellular growth, cytoskeletal modifications and synaptic function. Proteomic research may be useful in exploring the complex underlying molecular mechanisms of MA dependence.
Faure
J J
JJ
Division of Medical Physiology, University of Stellenbosch, Tygerberg, South Africa. jfaure@sun.ac.za
Hattingh
S M
SM
Stein
D J
DJ
Daniels
W M
WM
D033942:Adaptor Proteins, Vesicular Transport / Q000187:drug effects / Q000378:metabolism; D019969:Amphetamine-Related Disorders / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000187:drug effects / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000187:drug effects* / Q000378:metabolism; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000187:drug effects / Q000378:metabolism; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D016704:Synapsins / Q000187:drug effects / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D043222:Ubiquitin Thiolesterase / Q000187:drug effects / Q000378:metabolism; D051846:beta-Synuclein / Q000187:drug effects / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP
D033942:Adaptor Proteins, Vesicular Transport; D018765:Dopamine Uptake Inhibitors; D006360:Heat-Shock Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008694:Methamphetamine; D009419:Nerve Tissue Proteins; D010751:Phosphopyruvate Hydratase; D016704:Synapsins; D043222:Ubiquitin Thiolesterase; D051846:beta-Synuclein; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP; C095114:collapsin response mediator protein-2; C095993:complexin II; C496976:Uchl1 protein, mouse