title: Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues.
abstract: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-reaction of IBV RT-LAMP was found when tested with other viruses including Newcastle disease virus (NDV), avian reovirus (ARV), and infectious laryngotrachietis virus (ILTV) due to their mismatch with IBV RT-LAMP primers. A total of 187 clinical tissues samples (88 blood, 62 kidney and 37 lung) were evaluated and compared to conventional RT-PCR. The sensitivity of RT-LAMP and RT-PCR assays for detecting IBV RNA in clinical specimens was 99.5% and 98.4%, respectively. These findings showed that the RT-LAMP assay has potential usefulness for rapid and sensitive diagnosis in outbreak of IBV.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Hao-tai
authors.initials: HT
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Ma
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Ma
authors.forename: Yao-zhong
authors.initials: YZ
authors.lastname: Ding
authors.forename: Xiang-tao
authors.initials: XT
authors.lastname: Liu
authors.forename: Xue-peng
authors.initials: XP
authors.lastname: Cai
authors.forename: Li-qing
authors.initials: LQ
authors.lastname: Ma
authors.forename: Yong-guang
authors.initials: YG
authors.lastname: Zhang
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000821:virology*; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D001351:Infectious bronchitis virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases / Q000821:virology*; D048348:Reverse Transcription / Q000235:genetics*; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D017931:DNA Primers