title: Functional and neurochemical changes of the gastrointestinal tract in a rodent model of Parkinson's disease.
abstract: Patients with Parkinson's disease develop motor disturbances often accompanied by peripheral autonomic dysfunctions, including gastrointestinal disorders, such as dysphagia, gastric stasis and constipation. While the mechanisms subserving enteric autonomic dysfunctions are not clearly understood, they may involve the enteric dopaminergic and/or nitrergic systems. In the present study, we demonstrate that rats with unilateral 6-hydroxydopamine lesion of nigrostriatal dopaminergic neurons develop a marked inhibition of propulsive activity compared to sham-operated controls, as indicated by a 60% reduction of daily fecal output at the 4th week of observation. Immunohistochemical data revealed that 6-hydroxydopamine treatment did not affect the total number of HuC/D-positive myenteric neurons in both the proximal and distal segments of ileum and colon. Conversely, in the distal ileum and proximal colon the number of nitrergic neurons was significantly reduced. These results suggest that a disturbed distal gut transit, reminiscent of constipation in the clinical setting, may occur as a consequence of a reduced propulsive motility, likely due to an impairment of a nitric oxide-mediated descending inhibition during peristalsis.
authors.affiliation: Interdepartmental Research Center for Parkinson's Disease (CRIMP), Neurological Institute C. Mondino, Pavia, Pavia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Blandini
authors.forename: B
authors.initials: B
authors.lastname: Balestra
authors.forename: G
authors.initials: G
authors.lastname: Levandis
authors.forename: M
authors.initials: M
authors.lastname: Cervio
authors.forename: R
authors.initials: R
authors.lastname: Greco
authors.forename: C
authors.initials: C
authors.lastname: Tassorelli
authors.forename: M
authors.initials: M
authors.lastname: Colucci
authors.forename: M
authors.initials: M
authors.lastname: Faniglione
authors.forename: E
authors.initials: E
authors.lastname: Bazzini
authors.forename: G
authors.initials: G
authors.lastname: Nappi
authors.forename: P
authors.initials: P
authors.lastname: Clavenzani
authors.forename: S
authors.initials: S
authors.lastname: Vigneri
authors.forename: R
authors.initials: R
authors.lastname: De Giorgio
authors.forename: M
authors.initials: M
authors.lastname: Tonini
mesh_terms: D000818:Animals; D003106:Colon / Q000294:innervation / Q000503:physiopathology; D004195:Disease Models, Animal; D017615:Enteric Nervous System / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005767:Gastrointestinal Diseases / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology*; D005769:Gastrointestinal Motility / Q000502:physiology*; D007082:Ileum / Q000294:innervation / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D009473:Neuronal Plasticity / Q000502:physiology; D026602:Nitrergic Neurons / Q000378:metabolism / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D016627:Oxidopamine; D010300:Parkinson Disease / Q000150:complications*; D020734:Parkinsonian Disorders / Q000150:complications; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009569:Nitric Oxide; D016627:Oxidopamine