Liquid chromatography tandem mass spectrometry method for determination of bisoprolol in human plasma using d5-bisoprolol as the internal standard.
A simple, reliable and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) protocol was developed and validated for quantification of bisoprolol in human plasma. The sample was pretreated with a simple procedure of protein precipitation and an isotope-labeled d5-bisoprolol was used as internal standard. The chromatographic separation was performed on a Capcell Pak C(18) MG III column (100 mm x 2.0 mm, 5 microm). The protonated ion of the analyte was detected in positive ionization by multiple reaction monitoring mode. The mass transition pairs of m/z 326.3 --> 116.3 and m/z 331.3 --> 121.3 were used to detect bisoprolol and the internal standard, respectively. Linearity, accuracy, precision, recovery, matrix effect, dilution test and stability were evaluated during method validation over the range of 0.5-100 ng/mL. The validated method was successfully applied to analyze human plasma samples in a bisoprolol bioavailability study.
Liu
Gang-Yi
GY
Central Laboratory, Shanghai Xuhui Central Hospital,966 Huaihai Middle Road, Shanghai 200031, China.
Wang
Wei
W
Jia
Jing-Ying
JY
Lu
Chuan
C
Liu
Yan-Mei
YM
Zhang
Meng-Qi
MQ
Liu
Yun
Y
Li
Shui-Jun
SJ
Yu
Chen
C
D017298:Bisoprolol / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D006801:Humans; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
D017298:Bisoprolol