Demethylation of LHR in dehydroepiandrosterone-induced mouse model of polycystic ovary syndrome.
The cause of polycystic ovary syndrome (PCOS), a complex endocrine disorder, is unknown, but its familial aggregation implies underlying genetic influences. Hyperandrogenemia is regarded as a major endocrine character of the PCOS. In this study, we employed bisulfite sequencing and bisulfite restriction analysis to investigate the DNA methylation status of LHR, AR, FSHR and H19 in dehydroepiandrosterone (DHEA)-induced mouse PCOS model. The result showed that methylation of LHR was lost in ovary from induced PCOS mouse. However, AR, FSHR and H19 had similar methylation pattern in DHEA-treated group and control groups. These data provide evidence for close linkage between DNA demethylation of LHR and PCOS.
Zhu
Jia-Qiao
JQ
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Rd., Chaoyang, Beijing 100101, China.
Zhu
Liang
L
Liang
Xing-Wei
XW
Xing
Fu-Qi
FQ
Schatten
Heide
H
Sun
Qing-Yuan
QY
D000818:Animals; D019175:DNA Methylation / Q000235:genetics*; D003687:Dehydroepiandrosterone; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000235:genetics; D011962:Receptors, FSH / Q000235:genetics; D011974:Receptors, LH / Q000235:genetics*
D011944:Receptors, Androgen; D011962:Receptors, FSH; D011974:Receptors, LH; D003687:Dehydroepiandrosterone