Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on Biosilicate scaffolds.
OBJECTIVE
The purpose of this study was (i) to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings, and (ii) to determine whether the application of laser phototherapy at 830 nm would result in osteoblast proliferation on the glass-ceramic scaffold.


BACKGROUND
The use of bioscaffolds is considered a promising strategy for a number of clinical applications where tissue healing is sub-optimal. As in vitro osteoblast growth is a slow process, laser phototherapy could be used to stimulate osteoblast proliferation on bioscaffolds.


METHODS
A methodology was developed to seed an osteoblastic (MC3T3) cell line onto a novel glass-ceramic scaffold. Seeded scaffolds were irradiated with a single exposure of 830 nm laser at 10 J/cm(2) (at diode). Non-irradiated seeded scaffolds acted as negative controls. Cell proliferation was assessed seven days after irradiation.


RESULTS
Osteoblastic MC3T3 cells were successfully grown on discs composed of a glass-ceramic composite. Laser irradiation produced a 13% decrease in MC3T3 cell proliferation on glass-ceramic discs (mean +/- SD = 0.192 +/- 0.002) compared with control (non-irradiated) discs (mean +/-SD = 0.22 +/- 0.002).


CONCLUSIONS
Despite successful seeding of bioscaffolds with osteoblasts, laser phototherapy resulted in a reduction in cell growth compared to non-irradiated controls. Future research combining laser phototherapy and glass-ceramic scaffolds should take into account possible interactions of the laser with matrix compounds.
Renno
Ana Claudia Muniz
AC
Department of Bioscience, Federal University of São Paulo, Santos, SP, Brazil. a.renno@unifesp.br
McDonnell
Pauline Ann
PA
Crovace
Murilo Camuri
MC
Zanotto
Edgar Dutra
ED
Laakso
Liisa
L
D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects*; D002516:Ceramics; D066298:In Vitro Techniques; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy / Q000379:methods*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism / Q000528:radiation effects*; D010012:Osteogenesis / Q000528:radiation effects; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; C022107:Bioglass