title: Prophylactic effects of short-term acupuncture on Zusanli (ST36) in Wistar rats with lipopolysaccharide-induced acute lung injury.
abstract: OBJECTIVE
  To evaluate the prophylactic effects of short-term manual acupuncture stimulation on Zusanli (ST36) on acute lung injury (ALI) caused by lipopolysaccharide instillation in Wistar rats.
  
  
  METHODS
  Thirty-two Wistar rats were randomized into 4 groups (n=8) classified by the absence or presence of lipopolysaccharide instillation [negative (NC) and positive control (PC), respectively], and performance of sham or real needle stimulation [sham (SA) or real (RA) acupuncture, respectively]. Manual acupuncture was performed daily for 5 minutes over four consecutive days (days 1 to 4). Lipopolysaccharide instillation was performed on day 4 after the last acupuncture stimulation. Systemic blood samples, bronchoalveolar lavage, and bone marrow blood sample were collected on day 5 to measure the count of leukocytes.
  
  
  RESULTS
  RA reduced the inflammatory response on ALI as indicated by blood cell count and bronchoalveolar lavage cell count. SA presented a minor efficacy to reduce inflammatory response in rats with experimental ALI. Bronchoalveolar lavage showed increased cell counts of rats in the PC group as compared with the NC group, and less but no significant differences in cell counts in the PC-RA group as compared with the PC-SA group.
  
  
  CONCLUSIONS
  Prophylactic manual needling stimulation of ST36 can mitigate ALI caused by lipopolysaccharide instillation in Wistar rats. Further studies should address the mechanisms of immune system response induced by needle stimulation of ST36 acupoint.
authors.affiliation: Laboratory of Human Motion Analysis, Augusto Motta University Center, Rio de Janeiro, Brazil. arthurde@profunisuam.edu.br
authors.forename: Arthurde de Sá
authors.initials: Ade S
authors.lastname: Ferreira
authors.forename: Januário Gomes Mourão e
authors.initials: JG
authors.lastname: Lima
authors.forename: Tatiana Pereira Teixeira
authors.initials: TP
authors.lastname: Ferreira
authors.forename: Camila Madeira Tavares
authors.initials: CM
authors.lastname: Lopes
authors.forename: Rubens
authors.initials: R
authors.lastname: Meyer
mesh_terms: D026881:Acupuncture; D055371:Acute Lung Injury / Q000628:therapy*; D000818:Animals; D004195:Disease Models, Animal; D007249:Inflammation; D008070:Lipopolysaccharides; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008070:Lipopolysaccharides