title: Imprint cytology detects floating Brachyspira in human intestinal spirochetosis.
abstract: Human intestinal spirochetosis is a colorectal infectious disease caused by 2 Brachyspira species. Its diagnosis is established by histology, culture, and polymerase chain reaction, but the value of cytologic examination in routine practice remains unclear. In this study, imprint cytology of biopsy specimens was examined for cytologic features specific to human intestinal spirochetosis. Specimens were obtained from 65 colorectal regions (1-3 regions from each case) in 25 ultrastructurally and/or genetically confirmed human intestinal spirochetosis cases (20 with Brachyspira aalborgi, 3 with B pilosicoli, 2 with both genotypes). In cytologic specimens, spirochetes tended to be floating freely within the mucus and intestinal fluid, whereas the "fringe formation" of spirochetes typically observed in histologic specimens was indistinct in cytologic specimens. Spirochetes were identified in 58 regions (89.2%) and 23 cases (92.0%) by cytology, against in 50 regions (76.9%) and 22 cases (88.0%) by histology (no significant differences). In 6 of 8 regions exhibiting positive cytology and negative histology, B pilosicoli was present within the mucus. Hence, B pilosicoli may tend to float in the mucus. In conclusion, cytologic examination would be useful for the routine identification of human intestinal spirochetosis, especially if B pilosicoli is involved. Further, we suggest the existence of differences in biological behavior between these spirochetes.
authors.affiliation: Division of Medicine, Japan Self Defense Forces Hospital Yokosuka, Yokosuka 237-0071, Japan. sogata@ndmc.ac.jp
authors.forename: Sho
authors.initials: S
authors.lastname: Ogata
authors.forename: Masaaki
authors.initials: M
authors.lastname: Higashiyama
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Adachi
authors.forename: Ichiyo
authors.initials: I
authors.lastname: Ohara
authors.forename: Junichiro
authors.initials: J
authors.lastname: Nishiyama
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okusa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeo
authors.forename: Kimiya
authors.initials: K
authors.lastname: Sato
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kawai
mesh_terms: D000328:Adult; D001706:Biopsy; D017400:Brachyspira / Q000235:genetics / Q000302:isolation & purification*; D003106:Colon / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D003584:Cytological Techniques; D004269:DNA, Bacterial / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D007410:Intestinal Diseases / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D009093:Mucus / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial