Association of functional gene polymorphism IL-1beta in patients with external apical root resorption.
BACKGROUND
External apical root resorption (EARR) is a frequent complication of orthodontic treatment. Interleukin 1beta (IL-1beta) is a potent stimulus for bone resorption and osteoclastic cell recruitment during orthodontic tooth movement. The purpose of this study was to assess the association between a polymorphism in the IL-1beta genes and EARR during orthodontic treatment.


METHODS
The sample included 61 Brazilian orthodontic patients, divided into 2 groups according to the presence (affected group, n = 23) or absence (control group, n = 38) of EARR in the central and lateral maxillary incisors in the posttreatment period. DNA was obtained from buccal swab cells. The polymorphism was analyzed by the polymerase chain reaction followed by digestion with restriction enzyme. The polymerase chain reaction products were analyzed in 10% polyacrylamide gel and stained with silver.


RESULTS
There were significant statistical differences (P <0.05) among the frequencies of the alleles and genotypes of the IL-1beta gene polymorphism between the affected and unaffected groups, suggesting that allele 1 predisposed the subjects to EARR (odds ratio = 4.0).


CONCLUSIONS
The polymorphism of the IL-1beta gene is associated with root resorption in the studied population.
Bastos Lages
Elizabeth Maria
EM
Department of Orthodontics, Dental School, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. bethlages@uai.com.br
Drummond
Alexandre Fortes
AF
Pretti
Henrique
H
Costa
Fernando Oliveira
FO
Lages
Eugênio José Pereira
EJ
Gontijo
Amanda Ianotta
AI
Miranda Cota
Luís Otávio
LO
Brito
Rui Barbosa
RB
D000293:Adolescent; D000483:Alleles; D001938:Brazil; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D007180:Incisor / Q000473:pathology; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008437:Maxilla / Q000473:pathology; D009811:Odontometry; D009971:Orthodontics, Corrective / Q000009:adverse effects; D011110:Polymorphism, Genetic / Q000235:genetics*; D016300:Radiography, Bitewing; D012391:Root Resorption / Q000209:etiology / Q000235:genetics*; D019227:Tooth Apex / Q000473:pathology*
D053583:Interleukin-1beta