Spatially resolved DNA brushes on a chip: gene activation by enzymatic cascade.
We assemble on a chip spatially resolved DNA polymer brushes with density that can be controlled along continuous gradients, from dilute to dense packing, with 20-30 nm between DNA molecules. Investigation of DNA digestion in a approximately 1 kb DNA brush showed that endonucleases can digest within the brush, yet their activity is impeded at high DNA density and for restriction sites near the bottom. Local gene activation-a form of switch-was then demonstrated by a digestion-ligation cascade between two spatially resolved DNA brushes, leading to the in situ expression of green fluorescent protein upon a diffusion-controlled DNA swapping.
Bar
Maya
M
Department of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel.
Bar-Ziv
Roy H
RH
D000327:Adsorption; D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000648:ultrastructure*; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
D004247:DNA; D003851:Deoxyribonucleases