[Preparation and characterization of a novel functional anti-human CD83 monoclonal antibody].
OBJECTIVE
To prepare and characterize a novel anti-human CD83 monoclonal mAb.


METHODS
Female BALB/c mice of 6-8 weeks old were immunized with CD83 transfectant (L929/CD83) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells.The hybridoma cells were screened with CD83 transfectant (L929/CD83 and 293/CD83) by FCM. The biological characteristics of antibody were investigated by rapid isotyping analysis, karyotype analysis, competitive inhibition test and Western blot.


RESULTS
One hybridoma cell line named 9D8 was obtained, which had the property of secreting anti-human CD83 monoclonal antibody steadily, This mAb specifically recognized CD83 molecule expressed on human mature DC, activated T cells, and tumor cell line Daudi, myeloma cell line 8226.This mAb can recognize a distinct epitope from commercial mAb (HB15e).


CONCLUSIONS
One hybridoma cell line has been developed successfully, which may lay a foundation for further study on the function of this molecule.
Gao
Chao
C
Institute of Medical Biotechnology, Soochow University, Suzhou 215007, China. ycgaochao@126.com
Zhong
Wei-Xue
WX
Zheng
Shu-Dan
SD
Chen
Li-Wen
LW
Zhu
Yi-Bei
YB
Zhang
Xue-Guang
XG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D000097802:CD83 Antigen
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen