Carotenogenesis in Haematococcus lacustris: role of protein tyrosine phosphatases.
In the present study, we examined the inhibitory effects of protein tyrosine phosphatase (PTPase) inhibitors, including sodium orthovanadate (SOV), ammonium molybdate (AM), and iodoacetamide (IA), on cell growth, accumulation of astaxanthin, and PTPase activity in the photosynthetic algae Haematococcus lacustris. PTPase activity was assayed spectrophotometrically and was found to be inhibited 60% to 90% after treatment with the inhibitors. SOV markedly abolished PTPase activity, significantly activating the accumulation of astaxanthin. These data suggest that the accumulation of astaxanthin in H. lacustris results from the concerted actions of several PTPases.
Park
Jae-Kweon
JK
Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
Tran
Phuong Ngoc
PN
Kim
Jeong-Dong
JD
Hong
Seong-Joo
SJ
Lee
Choul-Gyun
CG
D002338:Carotenoids / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D056890:Eukaryota / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007460:Iodoacetamide / Q000494:pharmacology; D008982:Molybdenum / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology
D002338:Carotenoids; D014638:Vanadates; D008982:Molybdenum; D017027:Protein Tyrosine Phosphatases; C022175:ammonium molybdate; D007460:Iodoacetamide