Levels of DNA adducts in the blood and follicular fluid of women undergoing in vitro fertilization treatment and its correlation with the pregnancy outcome.
This study is designed to investigate the impact of DNA damage on pregnancy and fertilization rate outcome in a sub-sample of women undergoing IVF treatment. Blood and follicular fluid samples (n = 60) were analyzed for DNA adducts. While no BPDE-DNA adducts were detected, other unknown lipophilic adducts were seen in blood and follicular fluid. Women who failed to achieve pregnancy had higher DNA adducts in follicular fluid than those who succeeded (p < 0.05). Follicular fluid cotinine levels were associated with DNA adduct levels in blood and follicular fluid (p < 0.05). Evaluation of DNA damage resulting from oxidative stress could have a role in predicting IVF success rate.
Al-Saleh
Iman
I
Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Saudi Arabia. iman@kfshrc.edu.sa
El-Doush
Inaam
I
Arif
Jamal
J
Coskun
Serdar
S
Jaroudi
Kamal
K
Al-Shahrani
Abdulaziz
A
El-Din Mohamed
Gamal
G
D000328:Adult; D003367:Cotinine / Q000032:analysis; D018736:DNA Adducts / Q000032:analysis / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry; D006801:Humans; D019216:Metals, Heavy / Q000097:blood; D018384:Oxidative Stress; D011247:Pregnancy; D011256:Pregnancy Outcome
D018736:DNA Adducts; D019216:Metals, Heavy; D003367:Cotinine