Characterization of tail sheath protein of giant bacteriophage phiKZ Pseudomonas aeruginosa.
The tail sheath protein of giant bacteriophage phiKZ Pseudomonas aeruginosa encoded by gene 29 was identified and its expression system was developed. Localization of the protein on the virion was confirmed by immunoelectron microscopy. Properties of gene product (gp) 29 were studied by electron microscopy, immunoblotting and limited trypsinolysis. Recombinant gp29 assembles into the regular tubular structures (polysheaths) of variable length. Trypsin digestion of gp29 within polysheaths or extended sheath of virion results in specific cleavage of the peptide bond between Arg135 and Asp136. However, this cleavage does not affect polymeric structure of polysheaths, sheaths and viral infectivity. Digestion by trypsin of the C-truncated gp29 mutant, lacking the ability to self-assemble, results in formation of a stable protease-resistant fragment. Although there is no sequence homology of phiKZ proteins to proteins of other bacteriophages, some characteristic biochemical properties of gp29 revealed similarities to the tail sheath protein of bacteriophage T4.
Kurochkina
Lidia P
LP
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia. lpk@ibch.ru
Aksyuk
Anastasia A
AA
Sachkova
Maria Yu
MY
Sykilinda
Nina N
NN
Mesyanzhinov
Vadim V
VV
D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D008969:Molecular Sequence Data; D017105:Pseudomonas Phages / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D017376:Viral Tail Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000914:Antibodies, Viral; D017376:Viral Tail Proteins