Histochemical study on the intestine goblet cells in cichlid and poecilid species (Teleostei).
Histochemical properties of intestine goblet cells in firemouth cichlid, zebra mbuna, freshwater angelfish and platyfish are described. Goblet cells occurred regularly in the epithelial cell layer throughout the entire intestine, they were strongly coloured by alcian blue at pH 2.5. This colour got gradually weaker when the pH was reduced, but still after alcian blue at pH 0.2 these cells displayed a distinct blue colour. When the goblet cells were treated with periodic acid-Schiff (PAS), they displayed a strong purple-magenta colour. The findings that a number of goblet cells displayed various colours between blue and purple-magenta when acidic alcian blue was followed by PAS, and between blue and red-brown when acidic alcian blue was followed by neutral red, may reflect different ages or stages of development and differentiation for these cells. However, such results may also suggest a true cellular heterogeneity in the present population of goblet cells, reflecting that the intestine mucus layer has a number of roles in teleosts like lubrication, protection, immunological defence, digestion and absorption. In the ferritin injected specimens of firemouth cichlid and platyfish, a number of macrophage-like cells in intestine wall displayed Prussian blue precipitations in tissue treated with acid ferrocyanide, suggesting that these cells play a cleansing role in the intestinal wall. No ferritin uptake was seen in the intestine goblet cells and eosinophilic granule cells.
Leknes
I L
IL
Faculty of Engineering and Science, Sogn og Fjordane University College, Box 133, N-6851 Sogndal, Norway. ingvar.leknes@hisf.no
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D044002:Bryophyta / Q000378:metabolism*; D023681:Cichlids / Q000033:anatomy & histology / Q000378:metabolism*; D004063:Digestion / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005247:Feeding Behavior / Q000502:physiology; D005293:Ferritins / Q000378:metabolism / Q000493:pharmacokinetics; D020397:Goblet Cells / Q000166:cytology / Q000378:metabolism*; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D010503:Periodic Acid-Schiff Reaction; D011036:Poecilia / Q000033:anatomy & histology / Q000378:metabolism; D011540:Prussian Blue Reaction; D013045:Species Specificity; D013194:Staining and Labeling
D005293:Ferritins