Feedback from peripheral musculature to central pattern generator in the neurogenic heart of the crab Callinectes sapidus: role of mechanosensitive dendrites.
The neurogenic heart of decapod crustaceans is a very simple, self-contained, model central pattern generator (CPG)-effector system. The CPG, the nine-neuron cardiac ganglion (CG), is embedded in the myocardium itself; it generates bursts of spikes that are transmitted by the CG's five motor neurons to the periphery of the system, the myocardium, to produce its contractions. Considerable evidence suggests that a CPG-peripheral loop is completed by a return feedback pathway through which the contractions modify, in turn, the CG motor pattern. One likely pathway is provided by dendrites, presumably mechanosensitive, that the CG neurons project into the adjacent myocardial muscle. Here we have tested the role of this pathway in the heart of the blue crab, Callinectes sapidus. We performed "de-efferentation" experiments in which we cut the motor neuron axons to the myocardium and "de-afferentation" experiments in which we cut or ligated the dendrites. In the isolated CG, these manipulations had no effect on the CG motor pattern. When the CG remained embedded in the myocardium, however, these manipulations, interrupting either the efferent or afferent limb of the CPG-peripheral loop, decreased contraction amplitude, increased the frequency of the CG motor neuron spike bursts, and decreased the number of spikes per burst and burst duration. Finally, passive stretches of the myocardium likewise modulated the spike bursts, an effect that disappeared when the dendrites were cut. We conclude that feedback through the dendrites indeed operates in this system and suggest that it completes a loop through which the system self-regulates its activity.
García-Crescioni
Keyla
K
Institute of Neurobiology, University of Puerto Rico, 201 Blvd. del Valle, San Juan, Puerto Rico 00901.
Fort
Timothy J
TJ
Stern
Estee
E
Brezina
Vladimir
V
Miller
Mark W
MW
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D003386:Brachyura / Q000502:physiology*; D003712:Dendrites / Q000502:physiology*; D025461:Feedback, Physiological / Q000502:physiology*; D005260:Female; D017952:Ganglia, Invertebrate / Q000502:physiology*; D006321:Heart / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D008465:Mechanoreceptors / Q000502:physiology; D009046:Motor Neurons / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D009206:Myocardium; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010507:Periodicity; D012026:Reflex, Stretch / Q000502:physiology