title: S-adenosylmethionine stimulates fatty acid metabolism-linked gene expression in porcine muscle satellite cells.
abstract: Evidence indicates that both S-adenosylmethionine (SAMe) metabolism and intramuscular fat are associated with insulin resistance and type II diabetes. However, it is still unknown whether SAMe have effects on intramuscular adipogenesis. The present study investigated the roles of SAMe in the adipogenic differentiation of porcine muscle satellite cells. Cells isolated from neonatal pig muscle were treated with different concentrations of SAMe (0, 0.5 and 1.0 mM) for 24 h, induced for a 9-day adipogenic differentiation and were finally stained by oil red O staining. The adipocyte determination and differentiation factor-1 (ADD1) and peroxisome proliferator-activated receptor gamma (PPARγ) mRNA and protein were stimulated by SAMe treatment in a dose-dependent manner. Lipoprotein lipase (LPL) mRNA and protein were enhanced in 1.0 mM treatment group, compared with the control. No significant difference was observed in the intracellular lipid content among treatments. These results provide evidence that SAMe may be associated with intramuscular adipogenesis and indicate a novel action of SAMe in fat metabolism.
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yue
authors.forename: Qian
authors.initials: Q
authors.lastname: Fang
authors.forename: JingDong
authors.initials: J
authors.lastname: Yin
authors.forename: DeFa
authors.initials: D
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics*; D008297:Male; D008716:Methionine Adenosyltransferase / Q000235:genetics / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D012436:S-Adenosylmethionine; D008716:Methionine Adenosyltransferase