The effect of the aqueous extract of Kohautia grandiflora on paracetamol-induced liver damage in albino rats.
This study was carried out to investigate the hepatoprotective effect of the aqueous extract of Kohautia grandiflora on paracetamol induced hepatotoxicity in rats. A total of 20 albino rats of the Wister strain weighing 120-180 g were used for the study. The animals were divided into 4 groups of 5-rats each [I-IV]. Groups I, II and III served as the normal, paracetamol and plant extract controls and were administered with normal saline, 500 mg/kg of paracetamol and 300 mg/kg of the plant extract respectively for 7 days while rats in group IV served as the treatment group and were pre-treated with 300 mg/kg of the plant extract respectively for 7 days before 500 mg/kg of paracetamol was administered on the 8th day. At the end of the experimental period, blood was obtained from each rat for the determination of serum levels of aspartate transaminase [AST], alanine transaminase [ALT], alkaline phosphatase [ALP], albumin and bilirubin. Biochemical analysis of the serum obtained showed a significant increase [P<0.05] in the levels of AST, ALT, ALP and albumin in rats administered with 500 mg/kg of paracetamol and 300 mg/kg of the extract respectively. Pre-treatment of the animals with the extract caused a decrease in the levels of these enzymes. Histopathological assessments of the liver sections of rats administered with 500 mg/kg of paracetamol and 300 mg/kg of the extract showed congestion of the venous sinusoids, necrosis, edema, mononuclear infiltration and cloudy swellings with the severity higher in the paracetamol treated group. Pre-treatment with 300 mg/kg of the extract revealed a slight hepatoprotection compared with the rats that were administered with paracetamol alone. This study has shown that the aqueous extract of Kohautia grandiflora possesses slight hepatoprotective property.
Garba
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Department of Human Anatomy, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria. saniwakawa@yahoo.co.uk
Sambo
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Bala
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D000082:Acetaminophen; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D019610:Cytoprotection; D004195:Disease Models, Animal; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D019666:Rubiaceae; D012709:Serum Albumin / Q000378:metabolism
D015415:Biomarkers; D010936:Plant Extracts; D012709:Serum Albumin; D000082:Acetaminophen; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin