Performance levels of four Latin American laboratories for the serodiagnosis of Chagas disease in Mexican sera samples.
In nearly all of the previous multicentre studies evaluating serological tests for Trypanosoma cruzi infection, sera samples from Central or South American countries have been used preferentially. In this work we compared the reliability of the serological tests using Mexican sera samples that were evaluated in four independent laboratories. This included a reference laboratory in Brazil and three participant laboratories, including one in Central America and two in Mexico. The kappa index between Brazilian and Honduran laboratories reached 1.0 and the index for the Mexican laboratories reached 0.94. Another finding of this study was that the source of antigen did not affect the performance of the serological tests.
Luquetti
Alejandro O
AO
Instituto de Patología Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania, Brazil.
Espinoza
Bertha
B
Martínez
Ignacio
I
Hernández-Becerril
Nidia
N
Ponce
Carlos
C
Ponce
Elisa
E
Reyes
Pedro A
PA
Hernández
Oscar
O
López
Ruth
R
Monteón
Victor
V
D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D001938:Brazil; D014355:Chagas Disease / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006721:Honduras; D006801:Humans; D007753:Laboratories / Q000592:standards*; D008800:Mexico; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards*; D014349:Trypanosoma cruzi / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan