Enlarged and prominent nucleus may be indicative of tetraploidy: a laboratory study of a rare near-tetraploidy in a child patient with acute myelogenous leukemia AML-M4.
Near-tetraploidy is a rare cytogenetic abnormality in myelocytic malignancies in children and its significance is unknown. To investigate the pathologic characteristics of a near-tetraploidy in a child with acute myelogenous leukemia (AML-M4), bone marrow smears were prepared for morphologic analysis. Bone marrow samples were collected at presentation for flow cytometry, prepared by short-term (24 h) unstimulated culture and R-banding for conventional cytogenetic assay. We have performed a multifactorial analysis of the laboratory test results. In this case, the chromosomal analysis (R-banding) demonstrated a near-tetraploidy. Combined with morphologic and immunophenotypic results, the diagnosis was established as acute myelogenous leukemia (AML-M4). Near-tetraploidy is an uncommon cytogenetic finding, and the experience of this case further emphasizes the importance of the laboratory diagnostic methods.
Zhang
Ji-Hong
JH
Department of Hematology/ Oncology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. zhangjihong_cmu@sina.com
Zheng
Ying-Chun
YC
Wang
Yun-Xiu
YX
Zhang
Jun-Yan
JY
Liu
Zhuo-Gang
ZG
D001853:Bone Marrow / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology*; D002648:Child; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015479:Leukemia, Myelomonocytic, Acute / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D011123:Polyploidy