Structure of yellow fever virus envelope protein domain III.
The structure of recombinant domain III of the envelope protein (rED3) of yellow fever virus (YFV), containing the major neutralization site, was determined using NMR spectroscopy. The amino acid sequence and structure of the YFV-rED3 shows differences from ED3s of other mosquito-borne flaviviruses; in particular, the partially surface-exposed BC loop where methionine-304 and valine-324 were identified as being critical for the structure of the loop. Variations in the structure and surface chemistry of ED3 between flaviviruses affect neutralization sites and may affect host cell receptor interactions and play a role in the observed variations in viral pathogenesis and tissue tropism.
Volk
David E
DE
Departments of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555-1157, USA.
May
Fiona J
FJ
Gandham
Sai H A
SH
Anderson
Anjenique
A
Von Lindern
Jana J
JJ
Beasley
David W C
DW
Barrett
Alan D T
AD
Gorenstein
David G
DG
D000595:Amino Acid Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics; D015005:Yellow fever virus / Q000737:chemistry* / Q000235:genetics
D012367:RNA, Viral; D014759:Viral Envelope Proteins