Preliminary investigation of seeding mesenchymal stem cells on biodegradable scaffolds for vascular tissue engineering in vitro.
We used epsilon-caprolactone/L-lactide (PCLA) as a biodegradable scaffold and bone marrow (BM) mesenchymal stem cells (MSCs) as seeding cells for vascular tissue engineering: we expected MSCs to grow in the scaffolds in a bioreactor. The MSCs we used were from the BM of dogs, and vascular scaffolds were carried out on the electrospinning process of PCLA copolymers. MSCs expressed CD44 and CD105 but did not express CD34 or CD14 at an identical time point. Scaffolds were nontoxic to cells and were favorable for the growth and migration of MSCs. After culture in a bioreactor with mechanical stimulation, cells completely covered the surfaces of PCLA scaffolds and penetrated or infiltrated into the inside of the scaffold structure.
Li
Chun-Min
CM
Department of Thoracic Cardiovascular Surgery, FuXing Hospital, Capital Medical University, Beijing, People's Republic of China.
Wang
Zhong-Gao
ZG
Gu
Yong-Quan
YQ
Dong
Jian-De
JD
Qiu
Rong-Xin
RX
Bian
Ce
C
Liu
Xiao-Fei
XF
Feng
Zeng-Guo
ZG
D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D004285:Dogs; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D011091:Polyesters; C505727:poly(epsilon-caprolactone-co-lactide)