Treatment with DNAse I fosters binding to nec PBMC of CRP.
CRP is an important inflammatory marker, however, CRP levels are relatively low in patients with SLE. In addition patients with SLE often display low activities and serum levels for DNase I and complement, respectively. Here we show that DNase I treatment of nec PBMC increased their binding of CRP. Consequently, reduced DNase I activity in patients with SLE may contribute to the impaired opsonisation by CRP of dead cells, exacerbating the clearance defect in SLE of apo and nec cells.
Janko
Christina
C
Department for Internal Medicine 3, Institute for Clinical Immunology, Friedrich-Alexander University of Erlangen-Nürnberg, Erlangen, Germany. christina.janko@uk-erlangen.de
Schorn
Christine
C
Weidner
Daniela
D
Sarter
Kerstin
K
Chaurio
Ricardo
R
Sheriff
Ahmed
A
Schett
Georg
G
Munoz
Luis E
LE
D002097:C-Reactive Protein / Q000378:metabolism*; D002478:Cells, Cultured; D003850:Deoxyribonuclease I / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D009336:Necrosis; D010587:Phagocytosis / Q000276:immunology
D002097:C-Reactive Protein; D003850:Deoxyribonuclease I