Evaluation of hepatitis C virus core antigen assays in detecting recombinant viral antigens of various genotypes.
A single substitution within the hepatitis C virus core antigen sequence, A48T, which is observed in approximately 30% of individuals infected with genotype 2a virus, reduces the sensitivity of a commonly used chemiluminescence enzyme immunoassay. Quantitation of the antigen is improved by using a distinct anticore antibody with a different epitope.
Saeed
Mohsan
M
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Suzuki
Ryosuke
R
Kondo
Madoka
M
Aizaki
Hideki
H
Kato
Takanobu
T
Mizuochi
Toshiaki
T
Wakita
Takaji
T
Watanabe
Haruo
H
Suzuki
Tetsuro
T
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics; D006526:Hepatitis C / Q000175:diagnosis* / Q000821:virology; D018936:Hepatitis C Antigens / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D014758:Viral Core Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
D018936:Hepatitis C Antigens; D011994:Recombinant Proteins; D014758:Viral Core Proteins