title: A cell polarity protein aPKClambda is required for eye lens formation and growth.
abstract: The organisation of individual cells into a functional three-dimensional tissue is still a major question in developmental biology. Modulation of epithelial cell shape is a critical driving force in forming tissues. This is well illustrated in the eye lens where epithelial cells elongate extensively during their differentiation into fibre cells. It is at the lens equator that epithelial cells elongate along their apical-basal axis. During this process the elongating epithelial cells and their earliest fibre cell derivatives remain anchored at their apical tips, forming a discrete region or modiolus, which we term the lens fulcrum. How this is achieved has received scant attention and is little understood. Here, we show that conditional depletion of aPKClambda, a central effector of the PAR polarity complex, disrupts the apical junctions in elongating epithelial cells so that the lens fulcrum fails to form. This results in disorganised fibre cell alignment that then causes cataract. Interestingly, aPKClambda depletion also promotes epithelial-mesenchymal transition of the lens epithelial cells, reducing their proliferation, leading ultimately to a small lens and microphthalmia. These observations indicate that aPKClambda, a regulator of polarity and apical junctions, is required for development of a lens that is the correct size and shape.
authors.affiliation: School of Biological and Biomedical Sciences, University of Durham, Durham, UK.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Kazunori
authors.initials: K
authors.lastname: Akimoto
authors.forename: Michael L
authors.initials: ML
authors.lastname: Robinson
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohno
authors.forename: Roy A
authors.initials: RA
authors.lastname: Quinlan
mesh_terms: D000818:Animals; D002386:Cataract / Q000201:enzymology; D016764:Cell Polarity; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007908:Lens, Crystalline / Q000196:embryology* / Q000201:enzymology; D051379:Mice; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D000099293:Protein Kinase C-lambda
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C; D000099293:Protein Kinase C-lambda