title: Cytogenetic, fluorescence in situ hybridization, and molecular characterization of chronic myeloid leukemia in chronic phase with four BCR/ABL1 fusion signals: a case report.
abstract: We report a case of chronic myeloid leukemia in chronic stage with 48 chromosomes and four BCR/ABL1 fusion signals on two out of three chromosomes 9 and two signals on the two Philadelphia chromosomes. These abnormalities were detected by both conventional cytogenetic analysis and metaphase and interphase fluorescence in situ hybridization studies in approximately 90% of the cells at diagnosis. Real-time-polymerase chain reaction studies on peripheral blood showed b3a2(p210) and e1a2(p190) BCR/ABL1 fusion transcripts. During treatment with imatinib, the patient was asymptomatic with hematological remission. Cytogenetic and fluorescence in situ hybridization analysis revealed that only 6.6% of cells had the initial majority line karyotype, with disappearance of the p210 but increased p190 transcript, which led to the treatment being changed. We discuss the implication of cytogenetic and molecular alterations in the patient's evolution and treatment.
authors.affiliation: Division of Genetics, Department of Pathology, V. Macarena University Hospital, Seville, Spain. mtvargas@us.es
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Vargas
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Portero
authors.forename: Alicia
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Juana
authors.initials: J
authors.lastname: Reyes
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernández-Novoa
mesh_terms: D000328:Adult; D020732:Cytogenetic Analysis; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016044:Fusion Proteins, bcr-abl