title: Changes in sympathetic activity in prion neuroinvasion.
abstract: Prion diseases are neurodegenerative diseases affecting humans and animals in which the infectious agent or prion is PrP(res), a protease-resistant conformer of the cell protein PrP. The natural transmission route of prion diseases is peripheral infection, with the lymphoreticular system (LRS) and peripheral nerves being involved in animal models of scrapie neuroinvasion and human prion diseases. To study the effects of PrP neuroinvasion on sympathetic nerve function, we measured plasma catecholamine levels, blood pressure, heart rate, and PrP tissue levels in intraperitoneally or intracerebrally infected mice. The results indicate a specific alteration in sympathetic nerve function because the levels of noradrenaline (but not adrenaline) were increased in the animals infected peripherally (but not in those infected intracerebrally) and correlated with increased blood pressure. These findings confirm that prion neuroinvasion uses the sympathetic nervous system to spread from the periphery to the central nervous system after invading the LRS.
authors.affiliation: Department of Pharmacology, Chemotherapy and Medical Toxicology, Faculty of Medicine and Surgery, University of Milan, Milan, Italy.
authors.forename: Gianpietro
authors.initials: G
authors.lastname: Bondiolotti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Rossoni
authors.forename: Maria
authors.initials: M
authors.lastname: Puricelli
authors.forename: Elena
authors.initials: E
authors.lastname: Formentin
authors.forename: Barbara
authors.initials: B
authors.lastname: Lucchini
authors.forename: Giorgio
authors.initials: G
authors.lastname: Poli
authors.forename: Wilma
authors.initials: W
authors.lastname: Ponti
authors.forename: Silvio R
authors.initials: SR
authors.lastname: Bareggi
mesh_terms: D000818:Animals; D001342:Autonomic Nervous System Diseases / Q000097:blood* / Q000503:physiopathology*; D001794:Blood Pressure / Q000502:physiology; D015153:Blotting, Western; D001921:Brain / Q000503:physiopathology; D004837:Epinephrine / Q000097:blood; D006339:Heart Rate / Q000502:physiology; D007150:Immunohistochemistry; D016014:Linear Models; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009638:Norepinephrine / Q000097:blood; D017096:Prion Diseases / Q000097:blood* / Q000503:physiopathology*; D011328:Prions / Q000378:metabolism; D011897:Random Allocation; D013154:Spleen / Q000503:physiopathology; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D011328:Prions; D009638:Norepinephrine; D004837:Epinephrine