Ros-mediated activation of NF-kappaB and Foxo during muscle disuse.
We examined reactive oxygen species as upstream activators of nuclear factor kappaB; (NF-kappaB) and forkhead box O (Foxo) in skeletal muscle during disuse atrophy. Catalase, an enzyme that degrades H2O2, was overexpressed in soleus muscles via plasmid injection prior to 7 days of hindlimb immobilization. The increased catalase activity abolished immobilization-induced transactivation of both NF-kappaB and Foxo and attenuated the loss of muscle mass. Thus, H2O2 may be an important initiator of these signaling pathways that lead to muscle atrophy.
Dodd
Stephen L
SL
Department of Applied Physiology and Kinesiology, University of Florida, P.O. Box 118205, Gainesville, Florida 32611, USA. sdodd@hhp.ufl.edu
Gagnon
Brittany J
BJ
Senf
Sarah M
SM
Hain
Brian A
BA
Judge
Andrew R
AR
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000502:physiology*; D005500:Follow-Up Studies; D051858:Forkhead Transcription Factors / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D020966:Muscular Disorders, Atrophic / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D015398:Signal Transduction
D051858:Forkhead Transcription Factors; D016328:NF-kappa B; D017382:Reactive Oxygen Species