Bacterial immobilization by adhesion onto agave-fiber/polymer foamed composites.
Adhesion of Pseudomonas putida F1 onto agave-fiber/recycled-polyethylene foamed composites was studied under different controlled conditions. The adhesion process was analyzed in batch experiments controlling factors such as pH, contact time, temperature, initial biomass concentration and ionic strength; and was verified by scanning electron microscopy (SEM). The number of adhered bacteria after the experimental time was determined by difference between concentration of suspended cells in NaCl solution contained in two different Erlenmeyer flasks, one of the flasks with composite pellets and the other one without them. The concentration of cells in each flask was obtained using the serial dilution technique. Experimental data analysis showed that adsorption follows first-order kinetics. And it was further corroborated to be an irreversible process. For the first time, an equation is proposed here to predict the correlation between adhered bacteria and aqueous pH. In addition to the obvious reuse of waste material, these results suggested that agave-fiber/polymer foamed composites could be used as support for bacterial immobilization to be applied, among others in environmental processes such as bioremediation and biofiltration of gases with almost limitless possibilities.
Robledo-Ortíz
J R
JR
Departamento de Ingeniería Química, Universidad de Guadalajara, Blvd. Gral. Marcelino García Barragán # 1451, Guadalajara, Jalisco, C.P. 44430, Mexico.
Ramírez-Arreola
D E
DE
Gomez
C
C
González-Reynoso
O
O
González-Núñez
R
R
D027882:Agave / Q000382:microbiology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018533:Biomass; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007700:Kinetics; D009994:Osmolar Concentration; D020959:Polyethylene / Q000494:pharmacology*; D016958:Pseudomonas putida / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D012965:Sodium Chloride; D020959:Polyethylene