Simultaneous determination of venlafaxine and its main active metabolite O-desmethyl venlafaxine in rat plasma by LC-MS/MS.
A simple, sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the analysis of venlafaxine (VX) and its major active metabolite O-desmethyl venlafaxine (ODV) in rat plasma using carbamazepine as an internal standard (IS). The analytes from the biological matrix were extracted by liquid-liquid extraction using tert-butyl methyl ether (TBME). The HPLC separation of the analytes was performed on a water symmetry C18 (150 x 4.6 mm i.d., 5 microm) column, using a 10 mM ammonium formate:methanol (20:80 v/v) as mobile phase. The calibration curve concentration range was 10.10 to 8000.00 ng/mL for VX and ODV with coefficient of determination above 0.9970. The lower limit of quantification (LLOQ) of VX and ODV were 3.35 and 3.86 ng/mL, respectively. The intra- and inter-day coefficients of variation were within 15%.
Shah
Gaurang R
GR
Department of Chemistry, Veer Narmad South Gujarat University, Udhna-Magdalla Road, Surat-395 007, Gujarat, India.
Thaker
Bharat T
BT
Surati
Kiran R
KR
Parabia
Minu H
MH
D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002138:Calibration; D002220:Carbamazepine / Q000097:blood; D002853:Chromatography, Liquid; D003511:Cyclohexanols / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000069468:Desvenlafaxine Succinate; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D000069470:Venlafaxine Hydrochloride
D003511:Cyclohexanols; D012997:Solvents; D002220:Carbamazepine; D000069470:Venlafaxine Hydrochloride; D000069468:Desvenlafaxine Succinate