title: Molecular interaction of α-synuclein with tubulin influences on the polymerization of microtubule in vitro and structure of microtubule in cells.
abstract: Microtubule dynamics is essential for many vital cellular processes such as in intracellular transport, metabolism, and cell division. Evidences demonstrate that α-synuclein may associate with microtubular cytoskeleton and its major component, tubulin. In the present study, the molecular interaction between α-synuclein and tubulin was confirmed by GST pull-down assay and co-immunoprecipitation. The interacting regions within α-synuclein with tubulin were mapped at the residues 60-100 of α-synuclein that is critical for the binding activity with tubulin. Microtubule assembly assays and sedimentation tests demonstrated that α-synuclein influenced the polymerization of tubulin in vitro, revealing an interacting region-dependent feature. Confocal microscopy detected that exposures of α-synuclein proteins inhibited microtubule formation in the cultured cells, with a length-dependent phenomenon. Our data highlight a potential role of α-synuclein in regulating the microtubule dynamics in neurons. The association of α-synuclein with tubulin may further provide insight into the biological and pathophysiological function of synuclein.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Rd 100, Beijing, 100052, People's Republic of China.
authors.forename: R M
authors.initials: RM
authors.lastname: Zhou
authors.forename: Y X
authors.initials: YX
authors.lastname: Huang
authors.forename: X L
authors.initials: XL
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.forename: G R
authors.initials: GR
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Tian
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: Y Y
authors.initials: YY
authors.lastname: Jing
authors.forename: C
authors.initials: C
authors.lastname: Gao
authors.forename: X P
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D006224:Cricetinae; D006801:Humans; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism*; D050505:Mutant Proteins / Q000378:metabolism; D009391:Nephelometry and Turbidimetry; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021381:Protein Transport; D011817:Rabbits; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012996:Solutions; D014162:Transfection; D014404:Tubulin / Q000302:isolation & purification / Q000378:metabolism*; D051844:alpha-Synuclein / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D011994:Recombinant Proteins; C497604:SNCA protein, human; D012996:Solutions; D014404:Tubulin; D051844:alpha-Synuclein