Antihypertensive effect of enzymatic hydrolysate of collagen and Gly-Pro in spontaneously hypertensive rats.
Continuous oral feeding of enzymatic hydrolysate of porcine skin collagen showed an antihypertensive effect in spontaneously hypertensive rats (SHRs). We isolated an angiotensin I-converting enzyme (ACE) inhibitory peptide, Gly-Phe-Hyp-Gly-Pro (IC(50)=91 microM), from the hydrolysate, but the ACE inhibitory activities of the other peptides isolated were weak. Although the ACE inhibitory activity of Gly-Pro (IC(50)=360 microM) was not potent, Gly-Pro exists in collagen as a large number of repeated sequences. We then examined the antihypertensive effect of Gly-Pro. Orally administered Gly-Pro at 500 mg/kg significantly decreased the blood pressure of SHRs, and at 50 mg/kg it also showed a tendency to lower the blood pressure. Oral administration of Gly-Phe-Hyp-Gly-Pro (10 or 30 mg/kg) also decreased the blood pressure of SHRs.
Ichimura
Toshiaki
T
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba Ibaraki, Japan. ichimura@ni.aist.go.jp
Yamanaka
Akiko
A
Otsuka
Tatsuro
T
Yamashita
Eiichi
E
Maruyama
Susumu
S
D000595:Amino Acid Sequence; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D003094:Collagen / Q000378:metabolism*; D004151:Dipeptides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004798:Enzymes / Q000378:metabolism*; D006868:Hydrolysis; D006973:Hypertension / Q000188:drug therapy / Q000503:physiopathology; D008297:Male; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D011918:Rats, Inbred SHR
D000959:Antihypertensive Agents; D004151:Dipeptides; D004798:Enzymes; D010446:Peptide Fragments; C015248:glycylproline; D003094:Collagen