title: Detection of prostate specific antigen with nanomechanical resonators.
abstract: In this work, we use arrays of nanomechanical resonators to detect prostate specific antigen (PSA), a protein biomarker associated with prostate cancer. The surfaces of our very thin, trampoline-like devices are functionalized for immunospecific capture of PSA molecules, and the mass of bound material can be detected as a reduction in the resonant frequency. Fetal bovine serum was spiked with known concentrations of PSA, and in conjunction with a nanoparticle-based sandwich assay, concentrations as low as 50 fg mL(-1), or 1.5 fM, could be detected from the realistic samples. The presence of non-specific proteins in the serum did not significantly affect the sensitivity of our assay.
authors.affiliation: School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Philip S
authors.initials: PS
authors.lastname: Waggoner
authors.forename: Madhukar
authors.initials: M
authors.lastname: Varshney
authors.forename: Harold G
authors.initials: HG
authors.lastname: Craighead
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002021:Buffers; D002417:Cattle; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D017430:Prostate-Specific Antigen / Q000097:blood*; D012015:Reference Standards; D044967:Serum / Q000737:chemistry; D013499:Surface Properties
chemical_list: D015415:Biomarkers; D002021:Buffers; D017430:Prostate-Specific Antigen