title: Antimicrobial and cytotoxic abietane diterpenoids from the roots of Meriandera benghalensis (Roxb.) Benth.
abstract: Four abietane diterpenoids were isolated from the methanolic extract of the roots of Meriandera benghalensis and tested for their biological activity. Cryptotanshinone (2) and 17-hydroxycryptotanshinone (4) are known metabolites however the occurrence of tanshinone IIA (1) and przewaquinone A (3) from Meriandera benghalensis is reported for the first time. The four diterpenoids were identified by MS and one- and two dimensional NMR experiments. The isolated compounds were tested for their in vitro antiproliferative activity against three human cancer cell lines (a lung cancer (A-427), a urinary bladder cancer (5637) and a breast cancer (MCF-7) cell line) and for their antibacterial effect against three Gram-positive bacterial strains. All four abietanes showed potent cytotoxic effect against all cancer cell lines (IC50 between 1 and 8 microM) as well as antibacterial effect against the bacteria tested (MIC values between 33 and 70 microM).
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Sana'a-University, Yemen. r_mothana@yahoo.com
authors.forename: R A A
authors.initials: RA
authors.lastname: Mothana
authors.forename: R
authors.initials: R
authors.lastname: Jansen
authors.forename: R
authors.initials: R
authors.lastname: Gruenert
authors.forename: P J
authors.initials: PJ
authors.lastname: Bednarski
authors.forename: U
authors.initials: U
authors.lastname: Lindequist
mesh_terms: D045784:Abietanes / Q000302:isolation & purification / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D018517:Plant Roots / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
chemical_list: D045784:Abietanes; D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic