title: Fam83h is associated with intracellular vesicles and ADHCAI.
abstract: Defects in FAM83H on human chromosome 8q24.3 cause autosomal-dominant hypocalcified amelogenesis imperfecta (ADHCAI). FAM83H does not encode a recognizable signal peptide, so we predicted that the Fam83h protein functions within the cell. We tested this hypothesis by constitutively expressing mouse Fam83h with green fluorescent protein (GFP) fused to its C-terminus in HEK293 and HeLa cell lines. Green fluorescent signal from the Fam83h-GFP fusion protein was associated with perinuclear vesicles, usually in the vicinity of the Golgi apparatus. No signal was observed within the nucleus. In addition, we identified FAM83H nonsense mutations in Hispanic (C1330C>T; p.Q444X) and Caucasian (c.1192C>T; p.Q398X) families with ADHCAI. We conclude that Fam83h localizes in the intracellular environment, is associated with vesicles, and plays an important role in dental enamel formation. FAM83H is the first gene involved in the etiology of amelogenesis imperfecta (AI) that does not encode a secreted protein.
authors.affiliation: Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 1011 N. University, Ann Arbor, MI 48109-1078, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.forename: M R P
authors.initials: MR
authors.lastname: Estrella
authors.forename: Y Y
authors.initials: YY
authors.lastname: Hu
authors.forename: H L
authors.initials: HL
authors.lastname: Chan
authors.forename: H D
authors.initials: HD
authors.lastname: Zhang
authors.forename: J-W
authors.initials: JW
authors.lastname: Kim
authors.forename: J P
authors.initials: JP
authors.lastname: Simmer
authors.forename: J C-C
authors.initials: JC
authors.lastname: Hu
mesh_terms: D000369:Aged, 80 and over; D000567:Amelogenesis Imperfecta / Q000235:genetics / Q000473:pathology*; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D018389:Codon, Nonsense / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D022162:Cytoplasmic Vesicles / Q000648:ultrastructure*; D003596:Cytosine; D005091:Exons / Q000235:genetics; D005260:Female; D005799:Genes, Dominant / Q000235:genetics; D005973:Glutamine / Q000235:genetics; D006056:Golgi Apparatus / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D007438:Introns / Q000235:genetics; D049408:Luminescent Agents; D008297:Male; D051379:Mice; D011506:Proteins / Q000032:analysis*; D013941:Thymine; D017001:Tooth Demineralization / Q000235:genetics / Q000473:pathology
chemical_list: D018389:Codon, Nonsense; D018388:Codon, Terminator; C527308:FAM83H protein, human; D049408:Luminescent Agents; D011506:Proteins; D005973:Glutamine; D049452:Green Fluorescent Proteins; D003596:Cytosine; D013941:Thymine