title: Association of functional gene polymorphism IL-1beta in patients with external apical root resorption.
abstract: BACKGROUND
  External apical root resorption (EARR) is a frequent complication of orthodontic treatment. Interleukin 1beta (IL-1beta) is a potent stimulus for bone resorption and osteoclastic cell recruitment during orthodontic tooth movement. The purpose of this study was to assess the association between a polymorphism in the IL-1beta genes and EARR during orthodontic treatment.
  
  
  METHODS
  The sample included 61 Brazilian orthodontic patients, divided into 2 groups according to the presence (affected group, n = 23) or absence (control group, n = 38) of EARR in the central and lateral maxillary incisors in the posttreatment period. DNA was obtained from buccal swab cells. The polymorphism was analyzed by the polymerase chain reaction followed by digestion with restriction enzyme. The polymerase chain reaction products were analyzed in 10% polyacrylamide gel and stained with silver.
  
  
  RESULTS
  There were significant statistical differences (P <0.05) among the frequencies of the alleles and genotypes of the IL-1beta gene polymorphism between the affected and unaffected groups, suggesting that allele 1 predisposed the subjects to EARR (odds ratio = 4.0).
  
  
  CONCLUSIONS
  The polymorphism of the IL-1beta gene is associated with root resorption in the studied population.
authors.affiliation: Department of Orthodontics, Dental School, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. bethlages@uai.com.br
authors.forename: Elizabeth Maria
authors.initials: EM
authors.lastname: Bastos Lages
authors.forename: Alexandre Fortes
authors.initials: AF
authors.lastname: Drummond
authors.forename: Henrique
authors.initials: H
authors.lastname: Pretti
authors.forename: Fernando Oliveira
authors.initials: FO
authors.lastname: Costa
authors.forename: Eugênio José Pereira
authors.initials: EJ
authors.lastname: Lages
authors.forename: Amanda Ianotta
authors.initials: AI
authors.lastname: Gontijo
authors.forename: Luís Otávio
authors.initials: LO
authors.lastname: Miranda Cota
authors.forename: Rui Barbosa
authors.initials: RB
authors.lastname: Brito
mesh_terms: D000293:Adolescent; D000483:Alleles; D001938:Brazil; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D007180:Incisor / Q000473:pathology; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008437:Maxilla / Q000473:pathology; D009811:Odontometry; D009971:Orthodontics, Corrective / Q000009:adverse effects; D011110:Polymorphism, Genetic / Q000235:genetics*; D016300:Radiography, Bitewing; D012391:Root Resorption / Q000209:etiology / Q000235:genetics*; D019227:Tooth Apex / Q000473:pathology*
chemical_list: D053583:Interleukin-1beta