2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans.
Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade.
Lim
Crystale Siew-Ying
CS
Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
Wong
Won-Fen
WF
Rosli
Rozita
R
Ng
Kee-Peng
KP
Seow
Heng-Fong
HF
Chong
Pei-Pei
PP
D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D007851:Dodecanol / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development*; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D005656:Fungal Proteins; D007851:Dodecanol; D056565:Sirtuin 2