[Genetic polymorphisms of 9 non-combined of DNA index system short tandem repeat loci in Guangdong Han population].
OBJECTIVE
To investigate the genetic polymorphisms and their forensic application of 9 non-combined of DNA index system (CODIS) short tandem repeat(STR) loci in Guangdong Han population.


METHODS
DNA samples from 500 unrelated individuals were extracted and amplified with fluorescence labeled multiplex PCR system. PCR products were separated and genotyped with capillary electrophoresis.


RESULTS
One hundred and fifteen alleles and 160 genotypes were observed in the 9 STR loci, respectively. The heterozygosity was 0.824-0.884, the discrimination power (DP) was 0.925-0.969 and the polymorphism information content (PIC) was 0.77-0.86, respectively. The distribution met the Hardy-Weinberg equilibrium (P > 0.05). The total discrimination power was 1.00 x 10(-13), the combined probability of exclusion for trio-paternity testing was 0.999989488. The combined probability of exclusion for duo-paternity testing was 0.873436.


CONCLUSIONS
The 9 STR loci are powerful and reliable for personal identification and paternity testing. They can be used as supplementary loci in fatherless (motherless) testing or cases with mutation events.
Zhang
Jin-xiang
JX
Department of Forensic Medicine, Sun Yat-sen College of Medical Science, Zhongshan University, Guangzhou, Guangdong, 510089, PR China.
Xue
Tian-yu
TY
Li
Hai-xia
HX
Sun
Hong-yu
HY
Cheng
Jian-ding
JD
D000483:Alleles; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D002681:China; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic