title: Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on Biosilicate scaffolds.
abstract: OBJECTIVE
  The purpose of this study was (i) to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings, and (ii) to determine whether the application of laser phototherapy at 830 nm would result in osteoblast proliferation on the glass-ceramic scaffold.
  
  
  BACKGROUND
  The use of bioscaffolds is considered a promising strategy for a number of clinical applications where tissue healing is sub-optimal. As in vitro osteoblast growth is a slow process, laser phototherapy could be used to stimulate osteoblast proliferation on bioscaffolds.
  
  
  METHODS
  A methodology was developed to seed an osteoblastic (MC3T3) cell line onto a novel glass-ceramic scaffold. Seeded scaffolds were irradiated with a single exposure of 830 nm laser at 10 J/cm(2) (at diode). Non-irradiated seeded scaffolds acted as negative controls. Cell proliferation was assessed seven days after irradiation.
  
  
  RESULTS
  Osteoblastic MC3T3 cells were successfully grown on discs composed of a glass-ceramic composite. Laser irradiation produced a 13% decrease in MC3T3 cell proliferation on glass-ceramic discs (mean +/- SD = 0.192 +/- 0.002) compared with control (non-irradiated) discs (mean +/-SD = 0.22 +/- 0.002).
  
  
  CONCLUSIONS
  Despite successful seeding of bioscaffolds with osteoblasts, laser phototherapy resulted in a reduction in cell growth compared to non-irradiated controls. Future research combining laser phototherapy and glass-ceramic scaffolds should take into account possible interactions of the laser with matrix compounds.
authors.affiliation: Department of Bioscience, Federal University of São Paulo, Santos, SP, Brazil. a.renno@unifesp.br
authors.forename: Ana Claudia Muniz
authors.initials: AC
authors.lastname: Renno
authors.forename: Pauline Ann
authors.initials: PA
authors.lastname: McDonnell
authors.forename: Murilo Camuri
authors.initials: MC
authors.lastname: Crovace
authors.forename: Edgar Dutra
authors.initials: ED
authors.lastname: Zanotto
authors.forename: Liisa
authors.initials: L
authors.lastname: Laakso
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects*; D002516:Ceramics; D066298:In Vitro Techniques; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy / Q000379:methods*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism / Q000528:radiation effects*; D010012:Osteogenesis / Q000528:radiation effects; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass