title: Adoptive immunotherapy with liver allograft-derived lymphocytes induces anti-HCV activity after liver transplantation in humans and humanized mice.
abstract: After liver transplantation in HCV-infected patients, the virus load inevitably exceeds pre-transplantation levels. This phenomenon reflects suppression of the host-effector immune responses that control HCV replication by the immunosuppressive drugs used to prevent rejection of the transplanted liver. Here, we describe an adoptive immunotherapy approach, using lymphocytes extracted from liver allograft perfusate (termed herein liver allograft-derived lymphocytes), which includes an abundance of NK/NKT cells that mounted an anti-HCV response in HCV-infected liver transplantation recipients, despite the immunosuppressive environment. This therapy involved intravenously injecting patients 3 days after liver transplantation with liver allograft-derived lymphocytes treated with IL-2 and the CD3-specific mAb OKT3. During the first month after liver transplantation, the HCV RNA titers in the sera of recipients who received immunotherapy were markedly lower than those in the sera of recipients who did not receive immunotherapy. We further explored these observations in human hepatocyte-chimeric mice, in which mouse hepatocytes were replaced by human hepatocytes. These mice unfailingly developed HCV infections after inoculation with HCV-infected human serum. However, injection of human liver-derived lymphocytes treated with IL-2/OKT3 completely prevented HCV infection. Furthermore, an in vitro study using genomic HCV replicon-containing hepatic cells revealed that IFN-gamma-secreting cells played a pivotal role in such anti-HCV responses. Thus, our study presents what we believe to be a novel paradigm for the inhibition of HCV replication in HCV-infected liver transplantation recipients.
authors.affiliation: Department of Surgery, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku,Hiroshima 734-8551, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ohira
authors.forename: Kohei
authors.initials: K
authors.lastname: Ishiyama
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Marlen
authors.initials: M
authors.lastname: Doskali
authors.forename: Yuka
authors.initials: Y
authors.lastname: Igarashi
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Tashiro
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hiraga
authors.forename: Michio
authors.initials: M
authors.lastname: Imamura
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Asahara
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohdan
mesh_terms: D000368:Aged; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D002478:Cells, Cultured; D016174:Hepacivirus / Q000276:immunology; D006526:Hepatitis C / Q000517:prevention & control*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology / Q000627:therapeutic use; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000494:pharmacology / Q000627:therapeutic use*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000637:transplantation*; D008099:Liver / Q000166:cytology / Q000276:immunology; D016031:Liver Transplantation / Q000379:methods; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D016853:Muromonab-CD3 / Q000494:pharmacology / Q000627:therapeutic use*; D055611:Natural Killer T-Cells / Q000187:drug effects / Q000637:transplantation*; D012367:RNA, Viral / Q000097:blood; D013997:Time Factors; D014184:Transplantation, Homologous / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D000998:Antiviral Agents; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D016853:Muromonab-CD3; D012367:RNA, Viral; D007371:Interferon-gamma