Rice gene network inferred from expression profiling of plants overexpressing OsWRKY13, a positive regulator of disease resistance.
Accumulating information indicates that plant disease resistance signaling pathways frequently interact with other pathways regulating developmental processes or abiotic stress responses. However, the molecular mechanisms of these types of crosstalk remain poorly understood in most cases. Here we report that OsWRKY13, an activator of rice resistance to both bacterial and fungal pathogens, appears to function as a convergent point for crosstalk among the pathogen-induced salicylate-dependent defense pathway and five other physiologic pathways. Genome-wide analysis of the expression profiles of OsWRKY13-overexpressing lines suggests that OsWRKY13 directly or indirectly regulates the expression of more than 500 genes that are potentially involved in different physiologic processes according to the classification of the Gene Ontology database. By comparing the expression patterns of genes functioning in known pathways or cellular processes of pathogen infection and the phenotypes between OsWRKY13-overexpressing and wild-type plants, our data suggest that OsWRKY13 is also a regulator of other physiologic processes during pathogen infection. The OsWRKY13-associated disease resistance pathway synergistically interacts via OsWRKY13 with the glutathione/glutaredoxin system and flavonoid biosynthesis pathway to monitor redox homeostasis and to putatively enhance the biosynthesis of antimicrobial flavonoid phytoalexins, respectively, in OsWRKY13-overexpressing lines. Meanwhile, the OsWRKY13-associated disease resistance pathway appears to interact antagonistically with the SNAC1-mediated abiotic stress defense pathway, jasmonic acid signaling pathway, and terpenoid metabolism pathway via OsWRKY13 to suppress salt and cold defense responses as well as to putatively retard rice growth and development.
Qiu
Deyun
D
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
Xiao
Jun
J
Xie
Weibo
W
Liu
Hongbo
H
Li
Xianghua
X
Xiong
Lizhong
L
Wang
Shiping
S
D000596:Amino Acids / Q000096:biosynthesis; D003517:Cyclopentanes / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006023:Glycoproteins / Q000235:genetics; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics*; D008715:Methionine / Q000096:biosynthesis; D009584:Nitrogen / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics; D020156:Salicylic Acid / Q000494:pharmacology; D012717:Sesquiterpenes; D012965:Sodium Chloride / Q000494:pharmacology; D013729:Terpenes / Q000378:metabolism; D013912:Threonine / Q000096:biosynthesis; D014157:Transcription Factors / Q000187:drug effects / Q000235:genetics; D000094162:Phytoalexins; D000099179:Myocilin
D000596:Amino Acids; D003517:Cyclopentanes; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D006023:Glycoproteins; D008715:Methionine; D009584:Nitrogen; D054883:Oxylipins; D010940:Plant Proteins; D020156:Salicylic Acid; D012717:Sesquiterpenes; D012965:Sodium Chloride; D013729:Terpenes; D013912:Threonine; D014157:Transcription Factors; D000094162:Phytoalexins; D000099179:Myocilin; C011006:jasmonic acid