Beta1 integrin-dependent engulfment of Yersinia enterocolitica by macrophages is coupled to the activation of autophagy and suppressed by type III protein secretion.
Autophagy is a central lysosomal degradation process that is essential for the maintenance of cellular homeostasis. Autophagy has furthermore emerged as integral part of the host immune response. Autophagic processes promote the separation and degradation of intracellular microorganisms which contributes to the development of innate and adaptive immunity. Some pathogenic microbes have therefore evolved mechanisms to evade or impede autophagy. We analyzed the effects of the enteropathogenic bacterium Yersinia enterocolitica on autophagy in macrophages. Yersiniae use a number of defined adhesins and secreted proteins to manipulate host immune responses. Our results showed that Y. enterocolitica defective in type III protein secretion efficiently activated autophagy in macrophages. Autophagy was mediated by the Yersinia adhesins invasin and YadA and particularly depended on the engagement of beta(1) integrin receptors. Several autophagy-related events followed beta(1) integrin-mediated engulfment of the bacteria including the formation of autophagosomes, processing of the marker protein LC3, redistribution of GFP-LC3 to bacteria-containing vacuoles, and the segregation of intracellular bacteria by autophagosomal compartments. These results provide direct evidence for the linkage of beta(1) integrin-mediated phagocytosis and autophagy induction. Multiple microbes signal through integrin receptors, and our results suggest a general principle by which the sensing of an extracellular microbe triggers autophagy. Owing to the importance of autophagy as host defense response, wild-type Y. enterocolitica suppressed autophagy by mobilizing type III protein secretion. The subversion of autophagy may be part of the Y. enterocolitica virulence strategy that supports bacterial survival when beta(1) integrin-dependent internalization and autophagy activation by macrophages are deleterious for the pathogen.
Deuretzbacher
Anne
A
Institute for Medical Microbiology, Virology, and Hygiene, University Medical Center Eppendorf, Hamburg, Germany.
Czymmeck
Nicole
N
Reimer
Rudolph
R
Trülzsch
Konrad
K
Gaus
Kristin
K
Hohenberg
Heinrich
H
Heesemann
Jürgen
J
Aepfelbacher
Martin
M
Ruckdeschel
Klaus
K
D018829:Adhesins, Bacterial / Q000502:physiology; D000818:Animals; D001343:Autophagy / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D019012:Integrin beta1 / Q000096:biosynthesis / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008809:Mice, Inbred C3H; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000502:physiology; D010957:Plasmids / Q000276:immunology; D021381:Protein Transport / Q000235:genetics / Q000276:immunology; D014774:Virulence / Q000235:genetics / Q000276:immunology; D015008:Yersinia enterocolitica / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D019012:Integrin beta1; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; C064832:YadA protein, Yersinia; D049452:Green Fluorescent Proteins