title: [Predominance of G9 rotavirus in Valencia and Castellón between 2005 and 2007].
abstract: BACKGROUND
  Rotavirus is the leading cause of acute gastroenteritis in young children worldwide. Effective vaccines to prevent rotavirus infections are currently available, although their clinical use is still limited, and rotavirus still causes many episodes of infantile gastroenteritis, mainly during the winter seasons.
  
  
  OBJECTIVE
  To characterise G (VP7) and P (VP4) genotypes of rotaviruses causing acute gastroenteritis in children and to determine the prevalence of genotype G9 rotavirus in three public health areas in the provinces of Valencia and Castellon.
  
  
  METHODS
  Five-hundred and forty-one stool samples were prospectively collected from infants and children with gastroenteritis in the period between October 2005 and September 2008. They were analysed for rotavirus by ELISA or by immunochromatography. G and P genotyping was performed by reverse transcription and PCR (RT-PCR).
  
  
  RESULTS
  G and P rotavirus genotypes were characterised in a total of 525 faecal samples (97%), resulting in a global predominance of strains G9P[8] (56.5%) and G1P[8] (29.9%). During the period of time studied, G9P[8] was the G/P combination most frequently detected during the rotavirus seasons 2005-2006 and 2006-2007, being present in 81.2% and 64.7% of the patients, respectively. However, during the 2007-2008 season, G1P[8] strains were the most frequently found (68.8%), with a sharp decrease in G9P[8] strains to 7.2% of the samples.
  
  
  CONCLUSIONS
  Rotavirus G9P[8] have spread rapidly and widely during the 2005-2006 and 2006-2007 seasons, replacing other previously dominant genotypes (G1, G4) in our geographic area. Its incidence has declined sharply in 2007-2008, in which G1P[8] was again the predominating genotype.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Valencia, Valencia, España.
authors.forename: C J
authors.initials: CJ
authors.lastname: Téllez Castillo
authors.forename: R
authors.initials: R
authors.lastname: Montava Vilaplana
authors.forename: M
authors.initials: M
authors.lastname: Fernández Jiménez
authors.forename: J M
authors.initials: JM
authors.lastname: Ribes Fernández
authors.forename: J
authors.initials: J
authors.lastname: Buesa Gómez
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D011446:Prospective Studies; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D013030:Spain; D013997:Time Factors