title: Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells.
abstract: Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-gamma treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states.
authors.affiliation: Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan. amasumi@nih.go.jp
authors.forename: Atsuko
authors.initials: A
authors.lastname: Masumi
authors.forename: Isao
authors.initials: I
authors.lastname: Hamaguchi
authors.forename: Madoka
authors.initials: M
authors.lastname: Kuramitsu
authors.forename: Takuo
authors.initials: T
authors.lastname: Mizukami
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takizawa
authors.forename: Haruka
authors.initials: H
authors.lastname: Momose
authors.forename: Seishiro
authors.initials: S
authors.lastname: Naito
authors.forename: Kazunari
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001853:Bone Marrow; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D050836:Interferon Regulatory Factor-2 / Q000235:genetics / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D040201:Platelet Membrane Glycoprotein IIb / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D050836:Interferon Regulatory Factor-2; D040201:Platelet Membrane Glycoprotein IIb; D007371:Interferon-gamma