title: Mild sleep restriction acutely reduces plasma glucagon levels in healthy men.
abstract: BACKGROUND
  Sleep loss has repeatedly been suggested to affect glucose metabolism adversely, raising the question as to the impact of subtle forms of sleep loss.
  
  
  OBJECTIVE
  The aim of the study was to assess the effects of a single night of sleep restriction to 4.5 h on endocrine parameters of glucose metabolism.
  
  
  METHODS
  We conducted crossover, balanced experiments including two conditions, i.e. one night of 4.5 h and one night of 7 h of sleep.
  
  
  METHODS
  In 10 healthy men, circulating concentrations of insulin, C-peptide, epinephrine, norepinephrine, GH, ACTH, cortisol, and glucagon were measured after sleep and sleep restriction, respectively, during basal rest and a subsequent stepwise hypoglycemic clamp.
  
  
  RESULTS
  Mild sleep restriction induced a robust reduction in basal plasma glucagon levels that persisted throughout the hypoglycemic clamp (P < 0.03). Basal glucose, insulin, and C-peptide levels were unaffected by sleep restriction. Also, basal and hypoglycemia-stimulated concentrations of epinephrine, norepinephrine, and GH were unchanged after sleep restriction. Concentrations of ACTH (P < 0.05) and cortisol (P < 0.001) were reduced after sleep loss during baseline and at the start of hypoglycemia, but reached roughly comparable levels in both conditions at the end of the clamp (ACTH, P > 0.06; cortisol, P > 0.93).
  
  
  CONCLUSIONS
  Our data show that mild restriction of nocturnal sleep to 4.5 h has a reducing effect on circulating glucagon levels. This finding provides further evidence for the notion that glucose homeostasis is sensitive to subtle changes in sleep duration.
authors.affiliation: Department of Internal Medicine I, University of Luebeck, D-23538 Luebeck, Germany.
authors.forename: Sebastian M
authors.initials: SM
authors.lastname: Schmid
authors.forename: Kamila
authors.initials: K
authors.lastname: Jauch-Chara
authors.forename: Manfred
authors.initials: M
authors.lastname: Hallschmid
authors.forename: Bernd
authors.initials: B
authors.lastname: Schultes
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000378:metabolism; D018592:Cross-Over Studies; D005934:Glucagon / Q000097:blood*; D006728:Hormones / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D012892:Sleep Deprivation / Q000097:blood*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D006728:Hormones; D007328:Insulin; D005934:Glucagon