title: Formation of new blood vessels in the heart can be studied in cell cultures.
abstract: Controlled induction of the formation of new microvessels, i.e., therapeutic angiogenesis, may be used one day to treat patients that for example had suffered a myocardial infarction. Experimental models of angiogenesis in the heart in vivo substantially stress the animal. We therefore developed a model of angiogenesis of the heart in vitro, where mouse and rat heart pieces are stimulated under controlled conditions in a three dimensional matrix. Capillary-like sprouts emerging in these cultures represent early to midterm steps of angiogenesis and can be quantified to study potential angiogenic compounds and underlying mechanisms.
authors.affiliation: Department of Research, University Hospital Basel, Basel, Switzerland. rok.humar@unibas.ch
authors.forename: Rok
authors.initials: R
authors.lastname: Humar
authors.forename: Lourdes Sanchez
authors.initials: LS
authors.lastname: de Miguel
authors.forename: Fabrice N
authors.initials: FN
authors.lastname: Kiefer
authors.forename: Edouard J
authors.initials: EJ
authors.lastname: Battegay
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D000077214:Becaplermin; D002196:Capillaries / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005337:Fibrin / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005782:Gels; D006321:Heart / Q000502:physiology*; D006801:Humans; D051379:Mice; D009129:Muscle Tonus / Q000187:drug effects / Q000502:physiology; D009206:Myocardium / Q000166:cytology / Q000201:enzymology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009569:Nitric Oxide / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000172:deficiency; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D014664:Vasodilation / Q000187:drug effects
chemical_list: D043925:Angiogenesis Inducing Agents; D005782:Gels; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D016222:Fibroblast Growth Factor 2; D000804:Angiotensin II; D000077214:Becaplermin; D009569:Nitric Oxide; D005337:Fibrin; D052247:Nitric Oxide Synthase Type II