title: [Investigation of metabolic action of Cordyceps sinensis and its cultured mycelia on Escherichia coli by microcalorimetry].
abstract: This study is to investigate the effect of Cordyceps sinensis and its cultured mycelia on growth and metabolism of Escherichia coli, and microcalorimetric method was carried out to evaluate its biological activity. The study will provide the basis for the quality control of Cordyceps sinensis. Experimental result will show the effect of natural Cordyceps sinensis and its cultured mycelia on growth and metabolism of Escherichia coli, with index of P(1max) and effective rate (E) by microcalorimetry, the data of experiment were studied by cluster analysis. The results showed that Cordyceps sinensis and its cultured mycelia not only can promote growth and metabolism of Escherichia coli but also can regulate the balance of intestinal microecology efficiently. When the concentrations of samples > 6.0 mg mL(-1), natural Cordyceps sinensis can promote the growth and metabolism of Escherichia coli efficiently (P < 0.05) compared with the control group, and have better dose-effect relationship with concentration (r > 0.9), its cultured mycelia does not show conspicuous auxoaction (P > 0.05) and have not dose-effect relationship with concentration (r < 0.6); when the concentration of samples < 6.0 mg mL(-1), all samples does not show conspicuous auxoaction (P > 0.05). Natural Cordyceps sinensis and its cultured mycelia can be distinguished by cluster analysis. Microcalorimetry has a good prospect on the quality evaluation of the traditional Chinese medicine.
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Dan-Lei
authors.initials: DL
authors.lastname: Zhou
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Bao-Cai
authors.initials: BC
authors.lastname: Li
authors.forename: Yan-Shu
authors.initials: YS
authors.lastname: Wu
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D001688:Biological Products / Q000494:pharmacology*; D002151:Calorimetry / Q000379:methods; D032661:Cordyceps; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D008832:Microchemistry / Q000379:methods; D025282:Mycelium
chemical_list: D001688:Biological Products