[Identification and genetic analysis of pale-green mutant caused by Ds insertion in rice].
Ac/Ds insertion mutation was thought to be one of the powerful tools for identifying gene function in rice. In this study, the rice mutant with pale-green leaves was isolated from the population of Ds-T-DNA and Ac-T-DNA transgenic homozygote in a japonica rice variety Zhonghua 11. The leaves of the mutant turned to be pale-green at three-leaf stage. This mutant was capable of growing slowly and maturing under low-light conditions, but, rapidly died under natural light conditions. The analysis of the photosynthetic activity characteristics by measuring chlorophyll fluorescence in vivo suggested that the mutant was a typical photo-inhibitory mutant. Genetic analysis demonstrated that the mutation was the recessive one resulted from Ds insertion.
Zhang
Xiang-Qian
XQ
College of Agriculture, South China Agricultural University, Guangzhou, China. maizerice@scau.edu.cn
Liu
Fang
F
Zhu
Hai-Tao
HT
Li
Xiao-Yan
XY
Zeng
Rui-Zhen
RZ
D002734:Chlorophyll / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000379:methods*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
D004251:DNA Transposable Elements; D002734:Chlorophyll