Inhibitory effect of phytoglycoprotein (115 kDa) on the expression of TNF-alpha and interleukin-1beta via inhibition of MAP kinase in primary cultured mouse thymocytes.
The present study was performed to investigate the anti-inflammatory potential of 115 kDa glycoprotein isolated from Zanthoxylum piperitum DC leaves (ZPDC glycoprotein) in primary cultured mouse thymocytes. To determine whether the ZPDC glycoprotein has inhibitory capacity against inflammation in vitro, we evaluated the activities of inflammation-related factors such as phosphorylations of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) 1/2, and the activities of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in 12-O-tetradecanoylphorbol 13-acetata (PMA, 50 nM)-treated mouse thymocytes. Our results showed that the ZPDC glycoprotein (200 mug/ml) has a suppressive effect on the expression of MAPK (ERK1/2 and p38 MAPK), on mRNA expression of pro-inflammatory cytokines (TNF-alpha and IL-1beta), and on protein expression of pro-inflammatory proteins (iNOS and COX-2). We speculate that the ZPDC glycoprotein is an example of a natural compound that blocks pro-inflammatory signal transduction pathways.
Park
Cheol-Ho
CH
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, Yongbong-Dong, South Korea.
Oh
Phil-Sun
PS
Lim
Kye-Taek
KT
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006023:Glycoproteins / Q000737:chemistry / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000235:genetics; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D008970:Molecular Weight; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D032106:Zanthoxylum / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D006023:Glycoproteins; D053583:Interleukin-1beta; D010940:Plant Proteins; D047428:Protein Kinase Inhibitors; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D020928:Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate