title: Investigation of LDHA and COPB1 as candidate genes for muscle development in the MYOD1 region of pig chromosome 2.
abstract: Porcine MYOD1 gene has been mapped to swine chromosome (SSC) 2p14-p17, which is involved in the regulation of the proliferation and differentiation of skeletal muscle cells. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1) genes, which map close to MYOD1, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. However, little is known about the porcine LDHA and COPB1 genes. In the present study, the full-length cDNA of these two genes were cloned. The mapping results demonstrated that porcine LDHA and COPB1 were all mapped to SSC 2p14-p17. In this region, there are several QTL for growth and carcass traits, including average backfat thickness, lean and fat percentage. The RT-PCR results revealed that both LDHA and COPB1 were highly expressed in porcine skeletal muscle tissues, implying their potential regulatory function of muscle development. LDHA and COPB1 were then mapped to the region and multipoint analyses generated a best sex-averaged map order of each gene between linked markers: MYOD1_75.2 cM _LDHA_79 cM _CSRP3_83.8 cM _TEF-1_86.5 cM _COPB1_90 cM. Association analyses revealed that the substitution of c.423A>G had a significant effect on average daily gain on test, average backfat thickness (BFT), loin muscle area, lumbar BFT, marbling score, tenth rib BFT, average drip loss and fiber type II ratio. The substitution of c.3096C>T had a significant effect on average BFT, lumbar BFT, tenth rib BFT, carcass weight and last rib BFT. Interestingly, both SNPs were all associated with average BFT, lumbar BFT and tenth rib BFT.
authors.affiliation: Lab of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qiu
authors.forename: Xuewen
authors.initials: X
authors.lastname: Xu
authors.forename: Bing
authors.initials: B
authors.lastname: Fan
authors.forename: Max F
authors.initials: MF
authors.lastname: Rothschild
authors.forename: Yerle
authors.initials: Y
authors.lastname: Martin
authors.forename: Bang
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D020756:Coatomer Protein / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D005838:Genotype; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008460:Meat / Q000592:standards; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics*; D017570:MyoD Protein / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019655:Quantitative Trait, Heritable; D021101:Radiation Hybrid Mapping; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development
chemical_list: D020756:Coatomer Protein; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D007770:L-Lactate Dehydrogenase