Expression of the Arabidopsis thaliana histone gene AtHTA1 enhances rice transformation efficiency.
We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency.
Zheng
Y E
YE
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
He
Xiao-Wei
XW
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
Ying
Ying-Hui
YH
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
Lu
Jiang-Feng
JF
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
Gelvin
Stanton B
SB
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
Shou
Hui-Xia
HX
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China. Electronic address: huixia@zju.edu.cn.
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014170:Transformation, Genetic
D029681:Arabidopsis Proteins