Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity.
A small HSP gene, ZmsHSP, was isolated from Zea mays. Sequence analysis revealed that the open reading frame of ZmsHSP was 477 bp and that it encodes a protein composed of 159 amino acid residues with a calculated molecular mass of 18.17 kD and a predicated isoelectric point (pI) of 5.63. ZmsHSP contains a CS domain (p23-like domain) and shares similarity with the HSP90 co-chaperone p23. The expression level of ZmsHSP was different among various tissues with the highest expression in leaves and the lowest in silks. Results also showed that the expression of ZmsHSP in maize was significantly up-regulated by dehydration. Transgenic Arabidopsis plants overexpressing ZmsHSP under the control of the CaMV 35S promoter had lower endogenous cytokinin content and showed more sensitivity to cytokinin during the germination and early seedling stage than wild-type plants, suggesting that ZmsHSP might has a function in cytokinin response in Zea mays.
Cao
Zuping
Z
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Jia
Zhiwei
Z
Liu
Yongjun
Y
Wang
Meng
M
Zhao
Jinfeng
J
Zheng
Jun
J
Wang
Guoying
G
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D050888:Heat-Shock Proteins, Small / Q000235:genetics*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D003313:Zea mays / Q000235:genetics*
D003583:Cytokinins; D050888:Heat-Shock Proteins, Small; D010937:Plant Growth Regulators