title: Expression profile of a novel germ cell-specific gene, TSCPA, in mice and human.
abstract: In order to identify novel genes involved in spermatogenesis, testis cDNA samples from Balb/C mice of different postnatal days were hybridized with the whole mouse genome Affymetrix chip to screen the testis-specific genes. The characteristics of the selected genes were analyzed by RT-PCR as well as other bioinformatic tools. A novel differentially expressed testis-specific gene (GenBank Accession No: NM_029042) in the developmental stages of testes was identified, and named TSCPA. Cellular mapping prediction of TSCPA indicated that its protein was probably expressed in nuclei, and one putative domain (aa 332-377) was anchoring domain of cAMP-dependent type II PK. The result of subcellular localization of GFP-TSCPA fusion protein in Cos-7 cells showed that TSCPA protein was expressed in nuclei. RT-PCR analysis revealed that TSCPA was expressed specifically in mouse and human testis. TSCPA gene was expressed weakly in 21-day-old mouse testis and the expression was increased gradually from 38th day to 6th month of mouse testes. No expression of hTSCPA was found in cryptorchidism and Sertoli-cell-only syndrome patients. It was concluded that the expression profile of TSCPA in human and mice indicated that TSCPA might play an important role in spermatogenesis.
authors.affiliation: Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China. dongboyaa@yahoo.com.cn
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Aifa
authors.initials: A
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: Yaoting
authors.initials: Y
authors.lastname: Gui
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
chemical_list: D011506:Proteins