title: Interactions and oligomerization of hantavirus glycoproteins.
abstract: In this report the basis for the structural architecture of the envelope of hantaviruses, family Bunyaviridae, is systematically studied by the interactions of two glycoproteins N and C (Gn and Gc, respectively) and their respective disulfide bridge-mediated homo- and heteromeric oligomerizations. In virion extracts Gn and Gc associated in both homo- and hetero-oligomers which were, at least partially, thiol bridge mediated. Due to strong homo-oligomerization, the hetero-oligomers of Gn and Gc are likely to be mediated by homo-oligomeric subunits. A reversible pH-induced disappearance of a neutralizing epitope in Gc and dissociation of the Gn-Gc complex at pH values below 6.2 provide proteochemical evidence for the fusogenicity of Gc. Incomplete inactivation of virions at acidic pH indicates that additional factors are required for hantavirus fusion, as in the case of pestiviruses of the Flaviviridae. Based on similarities to class II fusion proteins, a structure model was created of hantavirus Gc using the Semliki Forest virus E1 protein as a template. In total, 10 binding regions for Gn were found by peptide scanning, of which five represent homotypic (Gn(I) to Gn(V)) and five represent heterotypic (Gc(I) to Gc(V)) interaction sites that we assign as intra- and interspike connections, respectively. In conclusion, the glycoprotein associations were compiled to a model wherein the surface of hantaviruses is formed of homotetrameric Gn complexes interconnected with Gc homodimers. This organization would create the grid-like surface pattern described earlier for hantaviruses in negatively stained electron microscopy specimens.
authors.affiliation: Department of Virology, University of Helsinki, Finland. jussi.hepojoki@helsinki.fi
authors.forename: Jussi
authors.initials: J
authors.lastname: Hepojoki
authors.forename: Tomas
authors.initials: T
authors.lastname: Strandin
authors.forename: Antti
authors.initials: A
authors.lastname: Vaheri
authors.forename: Hilkka
authors.initials: H
authors.lastname: Lankinen
mesh_terms: D001665:Binding Sites; D004220:Disulfides; D006023:Glycoproteins / Q000737:chemistry* / Q000502:physiology; D006476:Orthohantavirus / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008561:Membrane Fusion; D055503:Protein Multimerization; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000502:physiology; D053586:Virus Internalization
chemical_list: D004220:Disulfides; D006023:Glycoproteins; D014760:Viral Fusion Proteins