Visual observation of G-quadruplex DNA with the label-free fluorescent probe silole with aggregation-induced emission.
AIE molecule silole 1 could be used to detect G-quadruplex formation using an exonuclease I hydrolysis assay. This visual observation of G-quadruplexes has been successfully used in investigating multiple G-quadruplexes, including the one-stranded telomeric, c-myc, c-kit, VEGF G-quadruplexes, and a d(G(4)T(4)G(4)) inter-molecular G-quadruplex. The detection of G-quadruplex can be also applied to G-quadruplex isomers induced by small molecules.
Huang
Jing
J
College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan 430072, PR China.
Wang
Ming
M
Zhou
Yangyang
Y
Weng
Xiaocheng
X
Shuai
Liang
L
Zhou
Xiang
X
Zhang
Deqing
D
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005092:Exonucleases / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D054856:G-Quadruplexes; D016259:Genes, myc; D006801:Humans; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012821:Silanes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D016615:Telomere / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D005456:Fluorescent Dyes; D012821:Silanes; D042461:Vascular Endothelial Growth Factor A; D004247:DNA; D005092:Exonucleases