[Serovars of Salmonella enterica subspecies enterica and its antimicrobial resistance in slaughterhouse pigs].
A study was carried out in order to determine the prevalence of Salmonella and its serovars among porcine slaughterhouses, to evaluate the antimicrobial resistance profiles and to know the presence of class 1 integrons as possible reservoir of resistance. From a total of 386 samples from four porcine slaughterhouses of Buenos Aires and Santa Fe Provinces (Argentina), 93 (24.1%) Salmonella enterica subspecies enterica strains were identified, 52 (55.9%) from cecal contents and 41 (44.1%) from ileocecal lymph nodes. Thirteen serovars of S. enterica were found, the most prevalent were: S. Schwarzengrund, S. Heidelberg, S. subspecie I 6,8:e,h:-, S. Derby and S. Bredeney. Fifteen antimicrobials by the agar dilution method were tested: amikacin, gentamicin, ciprofloxacin, cephalotin, cefotaxime, enrofloxacin, fosfomycin, polimixin-B, tetracycline, chloramphenicol, streptomycin, trimethoprim-sulfamethoxazole, ampicillin, nitrofurantoin, and nalidixic acid. According to the CIM determination, 73% Salmonella enterica subspecies enterica strains were sensible to all the antimicrobials tested. Antimicrobial resistance was observed to tetracycline in 24 (25.8%) of 93 strains, to chloramphenicol in 22 (23.7%), to streptomycin in 22 (23.7%), to trimethoprim-sulfamethoxazole in 20 (21.5%), to ampicillin in 18 (19.4%), to nitrofurantoin in 3 (3.2%) and to nalidixic acid in 3 (3.2%). Some isolates of S. Typhimurium, S. Heidelberg, S. Derby, S. Orion showed multidrug resistance and carried the class 1 integrase gene. The highest percentage of resistance corresponded to the antimicrobials currently used in veterinary and porcine farms.
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Laboratorio de Diagnóstico e Investigaciones Bacteriológicas, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118 (1900) La Plata. mariela.ibar@fcv.unlp.edu.ar
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Piñeyro
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Caffer
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Quiroga
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Perfumo
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Centrón
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Giacoboni
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D000003:Abattoirs; D000822:Animal Husbandry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001118:Argentina; D002432:Cecum / Q000382:microbiology; D004197:Disease Reservoirs; D024901:Drug Resistance, Multiple, Bacterial; D005516:Food Microbiology; D005519:Food Preservation; D019426:Integrases / Q000235:genetics; D032023:Integrons / Q000235:genetics; D008198:Lymph Nodes / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D012034:Refrigeration; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012703:Serotyping; D034421:Sus scrofa / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D019426:Integrases