Effects of diammonium glycyrrhizinate on the pharmacokinetics of aconitine in rats and the potential mechanism.
1. The objective of this study was to investigate the effects of diammonium glycyrrhizinate on the pharmacokinetics of aconitine in rats and the potential mechanism. 2. After oral administration of diammonium glycyrrhizinate (50 mg kg(-1)), the peak plasma concentration (C(max)), area under the plasma concentration-time curve from zero to time tau (AUC(0-tau)), and absolute bioavailability of aconitine (0.2 mg kg(-1)) significantly increased 1.64-, 1.63- and 1.85-fold, respectively, but there was no significant change in half life (t(1/2)) or clearance (CL). In the other two routes of administration via the tail vein and hepatic portal vein, diammonium glycyrrhizinate (15 mg kg(-1)) did not affect any of the pharmacokinetic parameters of aconitine (0.02 mg kg(-1)). Thus, diammonium glycyrrhizinate can enhance the absorption of aconitine, leading to higher oral bioavailability and plasma levels, but it does not influence its elimination. 3. Moreover, an in vitro everted gut sac model and Ussing chamber model were used to investigate the potential mechanism. Results from bidirectional transport and inhibition studies demonstrated that P-glycoprotein was the main efflux transporter involved in the absorption of aconitine in rats. The absorption enhancement effect of diammonium glycyrrhizinate should be mainly attributed to inhibiting the activity of P-glycoprotein rather than to the influence on the paracellular or transcellular transport.
Chen
L
L
Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
Yang
J
J
Davey
A K
AK
Chen
Y-X
YX
Wang
J-P
JP
Liu
X-Q
XQ
D000157:Aconitine / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D000284:Administration, Oral; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism; D009596:Nitrophenols / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014700:Verapamil / Q000494:pharmacology
D004791:Enzyme Inhibitors; D026901:Membrane Transport Proteins; D009596:Nitrophenols; C002887:bis(4-nitrophenyl)phosphate; D019695:Glycyrrhizic Acid; D016572:Cyclosporine; D014700:Verapamil; D000157:Aconitine