Expression of PIN genes in rice (Oryza sativa L.): tissue specificity and regulation by hormones.
Twelve genes of the PIN family in rice were analyzed for gene and protein structures and an evolutionary relationship with reported AtPINs in Arabidopsis. Four members of PIN1 (designated as OsPIN1a-d), one gene paired with AtPIN2 (OsPIN2), three members of PIN5 (OsPIN5a-c), one gene paired with AtPIN8 (OsPIN8), and three monocot-specific PINs (OsPIN9, OsPIN10a, and b) were identified from the phylogenetic analysis. Tissue-specific expression patterns of nine PIN genes among them were investigated using RT-PCR and GUS reporter. The wide variations in the expression domain in different tissues of the PIN genes were observed. In general, PIN genes are up-regulated by exogenous auxin, while different responses of different PIN genes to other hormones were found.
Wang
Ji-Rong
JR
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Hu
Han
H
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Wang
Gao-Hang
GH
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Li
Jing
J
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Chen
Jie-Yu
JY
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Wu
Ping
P
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China. Electronic address: clspwu@zju.edu.cn.
D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007210:Indoleacetic Acids; D010940:Plant Proteins