Autoimmune response induced by dendritic cells exerts anti-tumor effect in murine model of leukemia.
An autoimmune response can be induced with the application of dendritic cells (DCs), offering a viable tumor vaccine for cancer immunotherapy. Previous studies have shown that DC-based tumor vaccines in animal tumor models can inhibit tumor growth and induce autoantibodies transiently without clinical or histological features of autoimmunity. The present study aimed to investigate the role of immune response induced by dendritic cells-based therapy, especially anti-ds DNA antibodies in tumor inhibition. In this study, high titers of anti-ds DNA antibodies were induced after injecting syngeneic dendritic cells into BALB/c mice. In addition, mice immunized with DCs showed the inhibition of RL male symbol 1, BALB/c leukemia cell line, tumor growth, and prolonged survival times of tumor mice but no significant difference was found in specific CTL response and NK cell activity when compared to those of the control group. Anti-ds DNA monoclonal antibodies can recognize RL male symbol 1 cells but not normal cells by FACS analysis. Monoclonal anti-ds DNA antibody was demonstrated to be able to lyse tumor cells via complement mediated reaction in vitro and also exhibits the anti-tumor effects when the antibody was injected into tumor-implanted mice. The data suggested that immunization with dendritic cells can induce autoimmune response, which might exert anti-tumor activity in vivo.
Tsai
Bor-Yu
BY
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
Lin
Yu-Li
YL
Chiang
Bor-Luen
BL
D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood; D015551:Autoimmunity / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D007167:Immunotherapy; D007938:Leukemia / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D016896:Treatment Outcome
D000974:Antibodies, Antinuclear; D019496:Cancer Vaccines