The E. coli single protein production system for production and structural analysis of membrane proteins.
At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.
Mao
Lili
L
Center for Advanced Technology and Medicine, UMDNJ-Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA.
Vaiphei
S Thangminlal
ST
Shimazu
Tsutomu
T
Schneider
William M
WM
Tang
Yuefeng
Y
Mani
Rajeswari
R
Roth
Monica J
MJ
Montelione
Gaetano T
GT
Inouye
Masayori
M
D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D008565:Membrane Proteins; D011506:Proteins