Identification of functional marker proteins in the mammalian growth cone.
Identification of proteins in the mammalian growth cone has the potential to advance our understanding of this critical regulator of neuronal growth and formation of neural circuit; however, to date, only one growth cone marker protein, GAP-43, has been reported. Here, we successfully used a proteomic approach to identify 945 proteins present in developing rat forebrain growth cones, including highly abundant, membrane-associated and actin-associated proteins. Almost 100 of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 17 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus their identification presents a significant step forward, providing marker proteins and candidate neuronal growth-associated proteins.
Nozumi
Motohiro
M
Divisions of Molecular Cell Biology and Ophthalmology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan.
Togano
Tetsuya
T
Takahashi-Niki
Kazuko
K
Lu
Jia
J
Honda
Atsuko
A
Taoka
Masato
M
Shinkawa
Takashi
T
Koga
Hisashi
H
Takeuchi
Kosei
K
Isobe
Toshiaki
T
Igarashi
Michihiro
M
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000032:analysis*; D051177:Catenins / Q000032:analysis / Q000235:genetics; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics; D009414:Nerve Growth Factors / Q000032:analysis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D016548:Prosencephalon / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D034622:RNA Interference; D051381:Rats; D055647:Rats, Transgenic; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D051177:Catenins; C413755:Dpysl5 protein, rat; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020543:Proteome; C085284:microtubule-associated protein 1B; D049452:Green Fluorescent Proteins