Alterations in mRNA expression of BACE1, cathepsin B, and glutaminyl cyclase in mice ischemic brain.
The relationship between cerebral ischemia and Alzheimer's disease has been evaluated extensively. However, the association between cerebral ischemia and the deposition of beta-amyloid (Abeta) remains to be clarified. Here, we used mice bilateral common carotid artery ligation model to investigate the alterations in mRNA expression of Abeta precursor protein cleavage enzyme 1(BACE1), cathepsin B, and glutaminyl cyclase after transient global cerebral ischemia. The reverse-transcriptase PCR assay showed that the expressions of these three Abeta-metabolism-related genes were upregulated in brain with different manner. It indicates that all these three Abeta-metabolism-related genes may participate in the acute and chronic Abeta generation after transient cerebral ischemia, and will be helpful to understand the mechanisms underlying the linkage of brain ischemia and Alzheimer's disease.
Ye
Jian-Tao
JT
Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, East of Waihuan Road, City of Universities, Guangzhou 510006, China.
Pi
Rong-Biao
RB
Mao
Xue-Xuan
XX
Chen
Xiao-Hong
XH
Qin
Jian
J
Xu
Suo-Wen
SW
Liu
Pei-Qing
PQ
D019881:Aminoacyltransferases / Q000235:genetics* / Q000378:metabolism; D053829:Amyloid Precursor Protein Secretases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics* / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002401:Cathepsin B / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism*
D012333:RNA, Messenger; D019881:Aminoacyltransferases; C059280:glutaminyl-peptide cyclotransferase; D053829:Amyloid Precursor Protein Secretases; D002401:Cathepsin B; D016282:Aspartic Acid Endopeptidases; C509282:Bace1 protein, mouse