Involvement of nitric oxide in the gastroprotective effect of ACEA, a selective cannabinoid CB1 receptor agonist, on aspirin-induced gastric ulceration.
The involvement of nitric oxide in the gastroprotective effect of ACEA (arachidonyl-2-chloroethylamide), a selective cannabinoid CB1 receptor agonist, on aspirin-induced gastric ulceration was studied in rats. ACEA (3 mg/kg i.p.) significantly reduced gastric ulcer formation. The gastroprotection of ACEA was attenuated by pretreatment with L-NAME (25 and 50 mg/kg i.p.), a nitric oxide synthase inhibitor. The combination of L-arginine (300 mg/kg i.v.), a precursor of nitric oxide with L-NAME (50 mg/kg i.p.) reversed the protective activity of ACEA (3 mg/kg i.p.). These results suggest that endogenous nitric oxide may be involved in the protective effect of ACEA.
Rutkowska
M
M
Department of Pharmacology, Medical University of Wrocław, Poland. rutkowsk@fa.am.wroc.pl
Fereniec-Gołebiewska
L
L
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D001095:Arachidonic Acids / Q000494:pharmacology*; D001120:Arginine / Q000494:pharmacology; D001241:Aspirin; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D005260:Female; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000627:therapeutic use; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D001095:Arachidonic Acids; D004791:Enzyme Inhibitors; D043884:Receptor, Cannabinoid, CB1; C119325:arachidonyl-2-chloroethylamide; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D001241:Aspirin; D019331:NG-Nitroarginine Methyl Ester