In vitro antimicrobial activity of several concentrations of NaOCl and Octenisept in elimination of endodontic pathogens.
OBJECTIVE
The objective of this study was to test and compare the in vitro effectiveness of sodium hypochlorite (NaOCl), and octenidine hydrochloride (Octenisept) at different concentrations in the elimination of resistant microorganisms including S. aureus, E. faecalis, and C. albicans over a range of time intervals.


METHODS
A broth dilution test was performed, and the timing for irrigants to kill microbial cells was recorded. Then the samples were compared by using Kruskal-Wallis test, with significance level at P less than .05. Also minimum inhibitory concentration (MIC) of Octenisept was evaluated.


RESULTS
The in vitro antimicrobial effect of the most effective concentrations of the tested irrigants were ranked from strongest to weakest as follows: 100% Octenisept, 50% Octenisept, 5.25 % NaOCl, and 2.5 % NaOCl.


CONCLUSIONS
The antimicrobial action is related to type and concentration of the irrigants as well as the microbial susceptibility.
Tirali
Resmiye Ebru
RE
Department of Pediatric Dentistry, Faculty of Dentistry, University of Gazi, Ankara, Turkey. ebru_aktepe@hotmail.com
Turan
Yeşim
Y
Akal
Neşe
N
Karahan
Zeynep Ceren
ZC
D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000187:drug effects; D007097:Imines; D007201:Indicator Dilution Techniques; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011725:Pyridines / Q000008:administration & dosage / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000008:administration & dosage / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
D000891:Anti-Infective Agents, Local; D007097:Imines; D011725:Pyridines; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; C034213:octenidine