title: Genome wide DNA-profiling of HIV-related B-cell lymphomas.
abstract: Non-Hodgkin lymphomas (NHL) represent a frequent complication of human immunodeficiency virus (HIV) infection. To elucidate HIV-NHL pathogenesis, we performed a genome-wide DNA profiling based on a single nucleotide polymorphism-based microarray comparative genomic hybridization in 57 HIV-lymphomas and, for comparison, in 105 immunocompetent diffuse large B-cell lymphomas (IC-DLBCL). Genomic complexity varied across HIV-NHL subtypes. HIV-Burkitt lymphoma showed a significantly lower number of lesions than HIV-DLBCL (P = 0.032), whereas the median number of copy number changes was significantly higher in Epstein-Barr virus negative (EBV-) HIV-DLBCL (42.5, range 8-153) compared to EBV+ cases (22; range 3-41; P = 0.029). Compared to IC-DLBCL, HIV-DLBCL displayed a distinct genomic profile with no gains of 18q and specific genetic lesions. Fragile sites-associated genes, including FHIT (FRA3B), WWOX (FRA16D), DCC (FRA18B) and PARK2 (FRA6E) were frequently inactivated in HIV-NHL by interstitial deletions, and a significantly higher prevalence of FHIT alterations was observed in HIV-DLBCL compared to IC-DLBCL. The same genes involved by fragile site deletions were also frequently affected by aberrant methylation of regulative regions.
authors.affiliation: Department of Clinical and Experimental Medicine & BRMA, "Amedeo Avogadro" University of Eastern Piedmont, Novara, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Capello
authors.forename: Marta
authors.initials: M
authors.lastname: Scandurra
authors.forename: Giulia
authors.initials: G
authors.lastname: Poretti
authors.forename: Paola M V
authors.initials: PM
authors.lastname: Rancoita
authors.forename: Michael
authors.initials: M
authors.lastname: Mian
authors.forename: Annunziata
authors.initials: A
authors.lastname: Gloghini
authors.forename: Clara
authors.initials: C
authors.lastname: Deambrogi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Martini
authors.forename: Davide
authors.initials: D
authors.lastname: Rossi
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Greiner
authors.forename: Wing C
authors.initials: WC
authors.lastname: Chan
authors.forename: Maurilio
authors.initials: M
authors.lastname: Ponzoni
authors.forename: Santiago M
authors.initials: SM
authors.lastname: Moreno
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Piris
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Canzonieri
authors.forename: Michele
authors.initials: M
authors.lastname: Spina
authors.forename: Umberto
authors.initials: U
authors.lastname: Tirelli
authors.forename: Giorgio
authors.initials: G
authors.lastname: Inghirami
authors.forename: Andrea
authors.initials: A
authors.lastname: Rinaldi
authors.forename: Emanuele
authors.initials: E
authors.lastname: Zucca
authors.forename: Riccardo D
authors.initials: RD
authors.lastname: Favera
authors.forename: Franco
authors.initials: F
authors.lastname: Cavalli
authors.forename: Luigi Maria
authors.initials: LM
authors.lastname: Larocca
authors.forename: Ivo
authors.initials: I
authors.lastname: Kwee
authors.forename: Antonino
authors.initials: A
authors.lastname: Carbone
authors.forename: Gianluca
authors.initials: G
authors.lastname: Gaidano
authors.forename: Francesco
authors.initials: F
authors.lastname: Bertoni
mesh_terms: D002051:Burkitt Lymphoma / Q000235:genetics; D002869:Chromosome Aberrations; D019175:DNA Methylation; D005787:Gene Frequency; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D046228:Microarray Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide