title: [Analysis of the extraction of red pellicle of walnut (Juglans regia L.) by ultraviolet-visible spectra and HPLC-ESI-MS(n)].
abstract: The extraction of the red pellicle of walnut (Juglans regia L.) was analyzed by UV-visible spectra and HPLC-ESI-MS(n) (high-performance-liquid-chromatography coupled with electrospray ionization mass spectrometry). The extraction in ethanol-HCl showed two absorption peaks at 560 and 591 nm respectively in the UV-Vis spectrum; after purified by lead acetate and thin-layer-chromatography, the extraction in ethanol-HCl showed 4 absorption peaks at 340, 370, 552 and 585 nm respectively. These results testified that the anthocyanin was in the extraction. Six molecular ion peaks (m/z) occurred on MS: 301, 481, 633, 783, 785 and 950, which was identified as ellagic acid, Hexahydroxydiphenoyl(HHDP)-glucose, Galloyl-HHDP-glucose, Di-HHDP-glucose, Di-Galloyl-HHDP-glucose, and HHDP-Valoneoyl-glucose respectively.
authors.affiliation: College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. wangkejian7212@263.net
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Wang
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Hao
authors.forename: Jian-Xun
authors.initials: JX
authors.lastname: Qi
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification; D031324:Juglans / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D019441:Plant Epidermis / Q000737:chemistry*; D059808:Polyphenols; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols