[YPK1 overexpression resulted salt stress hypersensitivity in Saccharomyces cerevisiae is dependent on TOR1].
Serine/threonine protein kinase YPK1, a homologue of mammalian protein kinase serum and glucocorticoid-inducible kinase (SGK), plays important physiological roles in Saccharomyces cerevisiae. It involves in cell wall maintenance, actin cytoskeleton dynamics, endocytosis, translation during nitrogen starvation and nutrient sensing.


OBJECTIVE
In order to further explore the cellular function of YPK1 and the its transduction pathway.


METHODS
We overexpressed YPK1 in wild type and tor1 mutant cells and monitored the cell growth responding to salt stress.


RESULTS
We found that YPK1 overexpression resulted salt stress hypersensitivity in wild type cells and this hypersensitivity was abolished by tor1 mutant.


CONCLUSIONS
Our results indicate that YPK1 may interact with target of rapamycin (TOR1) to regulate the salt stress response.
Cao
Hongping
H
Key Laboratory of Bio-resource and Eco-environment, Ministry of Education, School of Life Science, Sichuan University, Chengdu 610064, China. chp802800@sina.com
Wang
Yihui
Y
Zhong
Yan
Y
Liu
Xin
X
Miao
Ming
M
Duan
Wei
W
Liu
Ke
K
D015870:Gene Expression; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism*; D013312:Stress, Physiological
D038362:Glycogen Synthase Kinase 3; D019869:Phosphatidylinositol 3-Kinases; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; C083324:TOR1 protein, S cerevisiae; C068124:MCK1 protein, S cerevisiae