The use of DNA markers to map anthelmintic resistance loci in an intraspecific cross of Haemonchus contortus.
The use of DNA markers to track the development of anthelmintic resistance in parasites of livestock would allow informed choices for the management of this important problem. We describe a genetic mapping approach for the discovery of DNA markers for anthelmintic resistance in Haemonchus contortus. We crossed a multi-drug resistant field isolate of H. contortus with a well-characterized laboratory strain susceptible to 4 drug classes. The F2 were separately selected with 5 anthelmintics from 4 drug classes, producing drug-resistant populations carrying gene variants derived from both the field isolate and the laboratory strain. Individual F2 worms were analysed using amplicon length polymorphisms (ALPs). We looked for field isolate alleles over- or under-represented in F2 populations compared to the unselected F2 and/or the laboratory strain. The data we obtained suggest that marker association can be used to link neutral markers with resistance, but also that more markers and perhaps more inbred laboratory strains would make the procedure more likely to succeed.
Hunt
P W
PW
CSIRO, F.D. McMaster Laboratory, Chiswick, New England Highway, Armidale, N.S.W. 2350, Australia. Peter.Hunt@csiro.au
Kotze
A C
AC
Knox
M R
MR
Anderson
L J
LJ
McNally
J
J
LE Jambre
L F
LF
D000483:Alleles; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001315:Australia; D002874:Chromosome Mapping; D018509:DNA, Helminth / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D017238:Genes, Helminth; D056426:Genetic Loci; D005819:Genetic Markers; D006188:Haemonchiasis / Q000469:parasitology / Q000662:veterinary; D006189:Haemonchus / Q000187:drug effects* / Q000235:genetics; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000469:parasitology; D012757:Sheep Diseases / Q000469:parasitology
D000871:Anthelmintics; D018509:DNA, Helminth; D005819:Genetic Markers