title: Pharmacodynamic screening and simulation study of anti-hypoxia active fraction of xiangdan injection.
abstract: BACKGROUND
  The herbal formula Xiangdan injection and its modifications have been used in traditional Chinese medicine for about hundreds years to alleviate pain and promoting blood.
  
  
  OBJECTIVE
  To investigate the anti-hypoxia active fraction of Xiangdan injection.
  
  
  METHODS
  Xiangdan injection was extracted by ligarine, chloroform, acetic ether, n-butanol and water, represented respectively by A, B, C, D and E. Five extractions were group on the L12(2(5)) orthogonal designed table. NIH mices anti-hypoxia experiment under normal pressure with rats was adopted on the basis of orthogonal design, prediction pharmacodynamics model of TCM prescriptions was established, and the simulation bias was evaluated by combining two scatterplots. The anti-hypoxia active fraction of Xiangdan injection was determined by experiments.
  
  
  RESULTS
  The prediction model of TCM prescriptions established in this study can predict the drug actions for different formulas, and PE% <or= 25% was observed in 112 mice (93.3%).
  
  
  CONCLUSIONS
  As indicated in model prediction and experimental confirmation, the pharmacodynamic actions of the 5 formulas are all superior to that of the original formula, and ADE has the best effect.
authors.affiliation: Department of Traditional Chinese Medicinal Chemistry, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, People's Republic of China. szb8113@yahoo.com.cn
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Shen
authors.forename: Yong-Qin
authors.initials: YQ
authors.lastname: Yin
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Tang
authors.forename: Chao-Yan
authors.initials: CY
authors.lastname: Yan
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Li-Bing
authors.initials: LB
authors.lastname: Guo
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D005260:Female; D000860:Hypoxia / Q000188:drug therapy* / Q000401:mortality; D007274:Injections, Intraperitoneal; D008297:Male; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D012997:Solvents; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D012997:Solvents; C548756:xiangdan