title: Genevestigator transcriptome meta-analysis and biomarker search using rice and barley gene expression databases.
abstract: The wide-spread use of microarray technologies to study plant transcriptomes has led to important discoveries and to an accumulation of profiling data covering a wide range of different tissues, developmental stages, perturbations, and genotypes. Querying a large number of microarray experiments can provide insights that cannot be gained by analyzing single experiments. However, such a meta-analysis poses significant challenges with respect to data comparability and normalization, systematic sample annotation, and analysis tools. Genevestigator addresses these issues using a large curated expression database and a set of specifically developed analysis tools that are accessible over the internet. This combination has already proven to be useful in the area of plant research based on a large set of Arabidopsis data (Grennan, 2006). Here, we present the release of the Genevestigator rice and barley gene expression databases that contain quality-controlled and well annotated microarray experiments using ontologies. The databases currently comprise experiments from pathology, plant nutrition, abiotic stress, hormone treatment, genotype, and spatial or temporal analysis, but are expected to cover a broad variety of research areas as more experimental data become available. The transcriptome meta-analysis of the model species rice and barley is expected to deliver results that can be used for functional genomics and biotechnological applications in cereals.
authors.affiliation: Department of Biology, ETH Zurich, Universitaetstrasse 2, 8092 Zurich, Switzerland. phz@ethz.ch
authors.forename: Philip
authors.initials: P
authors.lastname: Zimmermann
authors.forename: Oliver
authors.initials: O
authors.lastname: Laule
authors.forename: Josy
authors.initials: J
authors.lastname: Schmitz
authors.forename: Tomas
authors.initials: T
authors.lastname: Hruz
authors.forename: Stefan
authors.initials: S
authors.lastname: Bleuler
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Gruissem
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D015415:Biomarkers / Q000032:analysis*; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D001467:Hordeum / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*; D012984:Software
chemical_list: D015415:Biomarkers