An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus.
Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.
Narvaez
Edgar
E
Laboratorio de Genética e Inmunologìa Molecular, Instituto de Biologìa, Facultad de Ciencias, Pontificia Universidad Católica de Valparaìso, Valparaìso, Chile.
Berendsen
Jorge
J
Guzmán
Fanny
F
Gallardo
José A
JA
Mercado
Luis
L
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D009092:Mucous Membrane / Q000276:immunology; D020317:Salmo salar / Q000276:immunology* / Q000382:microbiology; D012867:Skin / Q000276:immunology
D000907:Antibodies, Bacterial