title: [Expression of cyclin-dependent kinase 2-associated protein 1 in chicken embryos of different sexes].
abstract: To investigate the expression and functions of cyclin-dependent kinase 2-associated protein 1 (cdk2ap1) screened by suppression subtractive hybridization in chicken embryo development, a pair of primers was designed to amplify the cdk2ap1 fragment by RT-PCR and subsequently the fragment obtained was cloned into the plasmid pGEM-T. Sense and antisense probes labeled with digoxigenin were generated using SP6 and T7 RNA polymerases, respectively, and used to examine cdk2ap1 expression in chicken embryos of both sexes by whole-mount in situ hybridization. In both sexes, cdk2ap1 was expressed in the head mesenchyme, rhombencephalon, optic vesicles, spinal neural tube, and forelimb of 4.0-day-old embryos and the expression in males was significantly higher than that in females. In addition, in the genital ridge and hindlimb of the 4.0-day-old chicken embryo, cdk2ap1 was obviously expressed in the males but not in females. It is supposed that cdk2ap1 may play a role in the sexual differentiation and development of gonad of chicken embryo.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China. yang007yu@yahoo.com.cn
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Feng
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Pan
authors.initials: P
authors.lastname: Huang
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Li
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Peng
authors.forename: Yan-Zhang
authors.initials: YZ
authors.lastname: Gong
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000196:embryology / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D025521:Tumor Suppressor Proteins