A multicenter blinded study to evaluate KRAS mutation testing methodologies in the clinical setting.
Evidence that activating mutations of the KRAS oncogene abolish the response to anti-epidermal growth factor receptor therapy has revolutionized the treatment of advanced colorectal cancer. This has resulted in the urgent demand for KRAS mutation testing in the clinical setting to aid choice of therapy. The aim of this study was to evaluate six different KRAS mutation detection methodologies on two series of primary colorectal cancer samples. Two series of 80 frozen and 74 formalin-fixed paraffin-embedded tissue samples were sourced and DNA was extracted at a central site before distribution to seven different testing sites. KRAS mutations in codons 12 and 13 were assessed by using single strand conformation polymorphism analysis, pyrosequencing, high resolution melting analysis, dideoxy sequencing, or the commercially available TIB Molbiol (Berlin, Germany) or DxS Diagnostic Innovations (Manchester, UK) kits. In frozen tissue samples, concordance in KRAS status (defined as consensus in at least five assays) was observed in 66/80 (83%) cases. In paraffin tissue, concordance was 46/74 (63%) if all assays were considered or 71/74 (96%) using the five best performing assays. These results demonstrate that a variety of detection methodologies are suitable and provide comparable results for KRAS mutation analysis of clinical samples.
Whitehall
Vicki
V
Queensland Institute of Medical Research, Bancroft Building, 300 Herston Road, Herston Queensland 4029, Australia. Vicki.Whitehall@qimr.edu.au
Tran
Kayla
K
Umapathy
Aarti
A
Grieu
Fabienne
F
Hewitt
Chelsee
C
Evans
Tiffany-Jane
TJ
Ismail
Tuty
T
Li
Wei Qi
WQ
Collins
Peter
P
Ravetto
Paul
P
Leggett
Barbara
B
Salto-Tellez
Manuel
M
Soong
Richie
R
Fox
Stephen
S
Scott
Rodney J
RJ
Dobrovic
Alexander
A
Iacopetta
Barry
B
D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins / Q000235:genetics*
D003062:Codon; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins