title: Liquid chromatography tandem mass spectrometry method for determination of bisoprolol in human plasma using d5-bisoprolol as the internal standard.
abstract: A simple, reliable and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) protocol was developed and validated for quantification of bisoprolol in human plasma. The sample was pretreated with a simple procedure of protein precipitation and an isotope-labeled d5-bisoprolol was used as internal standard. The chromatographic separation was performed on a Capcell Pak C(18) MG III column (100 mm x 2.0 mm, 5 microm). The protonated ion of the analyte was detected in positive ionization by multiple reaction monitoring mode. The mass transition pairs of m/z 326.3 --> 116.3 and m/z 331.3 --> 121.3 were used to detect bisoprolol and the internal standard, respectively. Linearity, accuracy, precision, recovery, matrix effect, dilution test and stability were evaluated during method validation over the range of 0.5-100 ng/mL. The validated method was successfully applied to analyze human plasma samples in a bisoprolol bioavailability study.
authors.affiliation: Central Laboratory, Shanghai Xuhui Central Hospital,966 Huaihai Middle Road, Shanghai 200031, China.
authors.forename: Gang-Yi
authors.initials: GY
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Jia
authors.forename: Chuan
authors.initials: C
authors.lastname: Lu
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Liu
authors.forename: Meng-Qi
authors.initials: MQ
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Shui-Jun
authors.initials: SJ
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
mesh_terms: D017298:Bisoprolol / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D006801:Humans; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D017298:Bisoprolol