The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae.
A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.
Chen
Yong
Y
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
Li
Shuya
S
Xiong
Jian
J
Li
Zhenjiang
Z
Bai
Jianxin
J
Zhang
Lei
L
Ye
Qi
Q
Ouyang
Pingkai
P
Ying
Hanjie
H
D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D002384:Catalysis; D019343:Citric Acid / Q000378:metabolism* / Q000494:pharmacology; D002952:Citric Acid Cycle / Q000187:drug effects; D003470:Culture Media; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D009963:Orotic Acid / Q000737:chemistry / Q000378:metabolism; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000378:metabolism; D014542:Uridine Monophosphate / Q000096:biosynthesis* / Q000378:metabolism
D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D019343:Citric Acid; D009963:Orotic Acid; D002244:Carbon; D014542:Uridine Monophosphate; D005947:Glucose