Development and evaluation of a multiplex polymerase chain reaction assay to identify Salmonella serogroups and serotypes.
To improve limitations of Salmonella serotyping, 2 multiplex polymerase chain reaction (M-PCR) were developed using a strategy that identifies first the genes encoding serogroups (rfbJ, wzx). According to the serogroup determined, a second M-PCR identifies serotype (fliC, fljB, wcdB, and sdf-I sequence). Standardization and evaluation of both M-PCRs were carried out.
Cardona-Castro
Nora
N
Laboratorio de Microbiología, Instituto Colombiano de Medicina Tropical - Universidad CES Cra 43 A #52 Sur 99, Medellín, Colombia. ncardona@ces.edu.co
Sánchez-Jiménez
Miryan
M
Lavalett
Lelia
L
Múñoz
Nélida
N
Moreno
Jaime
J
D001426:Bacterial Proteins / Q000235:genetics; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012475:Salmonella / Q000145:classification* / Q000235:genetics; D012703:Serotyping / Q000379:methods*
D001426:Bacterial Proteins; D017931:DNA Primers