Association of XPD/ERCC2 G23591A and A35931C polymorphisms with skin lesion prevalence in a multiethnic, arseniasis-hyperendemic village exposed to indoor combustion of high arsenic coal.
More than 2,000 arsenic-related skin lesions (as at 2002) in a few villages of China's Southwest Guizhou Autonomous Prefecture represent a unique case of endemic arseniasis related with indoor combustion of high-arsenic coal. The skin lesion prevalence was significantly higher in ethnic Han villagers than in ethnic Hmong villagers. This study was focused on a possible involvement of XPD/ERCC2 G23591A and A35931C polymorphisms in risk modulation of skin lesions and in the body burden of As in this unique case of As exposure. G23591A and A35931C were genotyped by a PCR-based procedure. Total As contents in hair and urine samples as well as environmental samples of the homes of the two ethnic clans were analysed. A significant higher presentation of A/A35931 (homozygous wild) genotype in both clans was found in skin lesion patients, compared with their asymptomatic fellow villagers (67.1 vs. 46.3%, OR 2.36, 95% CI 1.35-4.14, P=0.002). Interestingly, the population frequencies of the A/A35931 genotype did not show significant differences between ethnic Han villagers and their Hmong neighbours (47.1 vs. 45.5%). Very low frequencies of homozygous and heterozygous variant genotypes of G23591A were recorded in the residents in target village. G/A23591 and A/A23591 were detected only in 3.2% (8/244) and 0.8% (2/244) of the villagers, respectively. The polymorphic status at the locus of A35931C might modulate the risk for arsenic-related skin lesions in the investigated groups.
Lin
Guo-fang
GF
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China.
Du
Hui
H
Chen
Ji-gang
JG
Lu
Hong-chao
HC
Guo
Wei-chao
WC
Golka
Klaus
K
Shen
Jian-hua
JH
D016902:Air Pollution, Indoor / Q000009:adverse effects; D001151:Arsenic / Q000032:analysis / Q000652:urine; D020261:Arsenic Poisoning / Q000150:complications / Q000453:epidemiology*; D001822:Body Burden; D002681:China / Q000453:epidemiology; D003031:Coal / Q000633:toxicity*; D019353:Endemic Diseases; D004784:Environmental Monitoring / Q000706:statistics & numerical data; D062665:Epidemiological Monitoring; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006197:Hair / Q000737:chemistry; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015995:Prevalence; D012424:Rural Population / Q000706:statistics & numerical data; D012871:Skin Diseases / Q000150:complications / Q000453:epidemiology* / Q000235:genetics; D012906:Smoke / Q000009:adverse effects; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
D003031:Coal; D012906:Smoke; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human; D001151:Arsenic