title: Identification of genes involved in stem rust resistance from wheat mutant D51 with the cDNA-AFLP technique.
abstract: Wheat (Triticum aestivum L.) stem rust caused by Puccinia graminis f. sp. tritici is one of the main diseases of wheat worldwide. Wheat mutant line D51, which was derived from the highly susceptible cultivar L6239, shows resistance to the prevailing races 21C3CPH, 21C3CKH, and 21C3CTR of P. graminis f. sp. tritici in China. In this study, we used the cDNA-AFLP technology to identify the genes that are likely involved in the stem rust resistance. EcoRI/MseI selective primers were used to generate approximately 1920 DNA fragments. Seventy five differentially transcribed fragments (3.91%) were identified by comparing the samples of 21C3CPH infected D51 with infected L6239 or uninfected D51. Eleven amplified cDNA fragments were sequenced. Eight showed significant similarity to known genes, including TaLr1 (leaf rust resistance gene), wlm24 (wheat powdery mildew resistance gene), stress response genes and ESTs of environment stress of tall fescue. These identified genes are involved in plant defense response and stem rust resistance and need further research to determine their usefulness in breeding new resistance cultivars.
authors.affiliation: College of Life Science, Northeast Forestry University, 150040 Harbin, China. yinjingjialei@yahoo.com.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Yin
authors.forename: Guangjin
authors.initials: G
authors.lastname: Wang
authors.forename: Jialei
authors.initials: J
authors.lastname: Xiao
authors.forename: Fengming
authors.initials: F
authors.lastname: Ma
authors.forename: Hongji
authors.initials: H
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yanling
authors.initials: Y
authors.lastname: Diao
authors.forename: Jinghua
authors.initials: J
authors.lastname: Huang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Dongjun
authors.initials: D
authors.lastname: Liu
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D015723:Gene Library; D017343:Genes, Plant / Q000502:physiology; D007113:Immunity, Innate / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology; D018547:Plant Stems / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics* / Q000276:immunology
chemical_list: D018076:DNA, Complementary