title: Redistribution of pulmonary blood flow impacts thermodilution-based extravascular lung water measurements in a model of acute lung injury.
abstract: BACKGROUND
  Studies using transthoracic thermodilution have demonstrated increased extravascular lung water (EVLW) measurements attributed to progression of edema and flooding during sepsis and acute lung injury. The authors hypothesized that redistribution of pulmonary blood flow can cause increased apparent EVLW secondary to increased perfusion of thermally silent tissue, not increased lung edema.
  
  
  METHODS
  Anesthetized, mechanically ventilated canines were instrumented with PiCCO (Pulsion Medical, Munich, Germany) catheters and underwent lung injury by repetitive saline lavage. Hemodynamic and respiratory physiologic data were recorded. After stabilized lung injury, endotoxin was administered to inactivate hypoxic pulmonary vasoconstriction. Computed tomographic imaging was performed to quantify in vivo lung volume, total tissue (fluid) and air content, and regional distribution of blood flow.
  
  
  RESULTS
  Lavage injury caused an increase in airway pressures and decreased arterial oxygen content with minimal hemodynamic effects. EVLW and shunt fraction increased after injury and then markedly after endotoxin administration. Computed tomographic measurements quantified an endotoxin-induced increase in pulmonary blood flow to poorly aerated regions with no change in total lung tissue volume.
  
  
  CONCLUSIONS
  The abrupt increase in EVLW and shunt fraction after endotoxin administration is consistent with inactivation of hypoxic pulmonary vasoconstriction and increased perfusion to already flooded lung regions that were previously thermally silent. Computed tomographic studies further demonstrate in vivo alterations in regional blood flow (but not lung water) and account for these alterations in shunt fraction and EVLW.
authors.affiliation: Department of Anesthesiology, Johns Hopkins Medical Institutes, Baltimore, Maryland 21287, USA. beasley@jhmi.edu
authors.forename: R Blaine
authors.initials: RB
authors.lastname: Easley
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Mulreany
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Lancaster
authors.forename: Jason W
authors.initials: JW
authors.lastname: Custer
authors.forename: Ana
authors.initials: A
authors.lastname: Fernandez-Bustamante
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Colantuoni
authors.forename: Brett A
authors.initials: BA
authors.lastname: Simon
mesh_terms: D055371:Acute Lung Injury / Q000503:physiopathology*; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D015633:Extravascular Lung Water; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000000981:diagnostic imaging; D011175:Positive-Pressure Respiration; D011652:Pulmonary Circulation; D013815:Thermodilution; D014057:Tomography, X-Ray Computed
chemical_list: D008070:Lipopolysaccharides