Measurement of total Zn and Zn isotope ratios by quadrupole ICP-MS for evaluation of Zn uptake in gills of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss).
This study evaluates the potential use of stable zinc isotopes in toxicity studies measuring zinc uptake by the gills of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss). The use of stable isotopes in such studies has several advantages over the use of radioisotopes, including cost, ease of handling, elimination of permit requirements, and waste disposal. A pilot study using brown trout was performed to evaluate sample preparation methods and the ability of a quadrupole inductively coupled plasma mass spectrometer (ICP-MS) system to successfully measure changes in the (67)Zn/(66)Zn ratios for planned exposure levels and duration. After completion of the pilot study, a full-scale zinc exposure study using rainbow trout was performed. The results of these studies indicate that there are several factors that affect the precision of the measured (67)Zn/(66)Zn ratios in the sample digests, including variations in sample size, endogenous zinc levels, and zinc uptake rates by individual fish. However, since these factors were incorporated in the calculation of the total zinc accumulated by the gills during the exposures, the data obtained were adequate for their intended use in calculating zinc binding and evaluating the influences of differences in water quality parameters.
Wolf
Ruth E
RE
US Geological Survey, Denver Federal Center, Denver, CO, USA. rwolf@usgs.gov
Todd
Andrew S
AS
Brinkman
Steve
S
Lamothe
Paul J
PJ
Smith
Kathleen S
KS
Ranville
James F
JF
D000465:Algorithms; D000818:Animals; D001692:Biological Transport; D004781:Environmental Exposure / Q000032:analysis; D005880:Gills / Q000378:metabolism*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010865:Pilot Projects; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis; D015032:Zinc / Q000032:analysis* / Q000493:pharmacokinetics; D015033:Zinc Isotopes / Q000032:analysis / Q000493:pharmacokinetics
D014874:Water Pollutants, Chemical; D015033:Zinc Isotopes; D015032:Zinc