title: A simple method to introduce marker-free genetic modifications into the chromosome of naturally nontransformable Bacillus amyloliquefaciens strains.
abstract: A simple method to introduce marker-free deletions, insertions, and point mutations into the chromosomes of naturally nontransformable Bacillus amyloliquefaciens strains has been developed. The method is efficient and fast, and it allows for the generation of genetic modifications without the use of a counter-selectable marker or a special prerequisite strain. This method uses the combination of the following: the effective introduction of a delivery plasmid into cells for gene replacement; a two-step replacement recombination procedure, which occurs at a very high frequency due to the use of a thermosensitive rolling-circle replication plasmid; and colony polymerase chain reaction (PCR) analysis for screening. Using PCR primers with mismatches at the 3' end enables the selection of strains that contain a single nucleotide substitution in the target gene. This approach can be used as a routine method for the investigation of complex physiological pathways and for the metabolic engineering of food-grade industrial B. amyloliquefaciens and other Bacillus strains.
authors.affiliation: Ajinomoto-Genetika Research Institute, Moscow, Russia. natalia_zakataeva@agri.ru
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Zakataeva
authors.forename: Oksana V
authors.initials: OV
authors.lastname: Nikitina
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Gronskiy
authors.forename: Dmitriy V
authors.initials: DV
authors.lastname: Romanenkov
authors.forename: Vitaliy A
authors.initials: VA
authors.lastname: Livshits
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial; D005819:Genetic Markers; D005822:Genetic Vectors; D042843:Glucose 1-Dehydrogenase / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005819:Genetic Markers; D008565:Membrane Proteins; D042843:Glucose 1-Dehydrogenase