title: Biopure MTAD Induces DNA Damage but Not Cellular Death: An In Vitro Study.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the capacity of BioPure MTAD to induce genetic damage in vitro. Genotoxicity was assessed by the single cell gel (comet) assay.
  
  
  METHODS
  Chinese hamster ovary (CHO) cells or murine fibroblasts cells were exposed to increasing final concentrations ranging from 0.1 a 10%. All treatments were performed for 1 hour at 37 degrees C. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 1 hour at 37 degrees C and the positive control group was treated with methylmetanesulfonate (at 1 muM) for 1 hour at 37 degrees C.
  
  
  RESULTS
  Present results showed that the BioPure MTAD was able to promote DNA breakage in CHO cells only at the highest concentration tested as well as to induce significant increase in tail moment at all tested concentrations in murine fibroblasts.
  
  
  CONCLUSIONS
  In summary, our results indicate that BioPure MTAD is a genotoxic agent as depicted by the single cell gel (comet) assay. (Eur J Dent 2009;3:285-289).
authors.affiliation: Department of Endodontics, School of Dentistry, Rio de Janeiro State University, UERJ, Rio de Janeiro RJ, Brazil.
authors.forename: Juliana Soares Roter
authors.initials: JS
authors.lastname: Marins
authors.forename: Luciana Moura
authors.initials: LM
authors.lastname: Sassone
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro