title: Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacteriumbifidum BIM B-465.
abstract: The composition and structure of cell wall polysaccharides of a biotechnologically promising probiotic strain of bifidobacteria Bifidobacteriumbifidum BIM B-465 were established by monosaccharide and methylation analyses along with 2D (1)H-(1)H homonuclear and (1)H-(13)C heteronuclear correlation NMR spectroscopy. The major polysaccharide represents a branched glucogalactan consisting of heptasaccharide repeating units having the following structure: -->6)-alpha-D-Glcp-(1-->3)-beta-D-Galf-(1-->3)-alpha-D-Glcp-(1-->2)-beta-D-Galf-(1-->3)-alpha-D-Galp-(1-->3)-alpha-D-Glcp-(1--> [structure: see the text]. The second isolated polysaccharide is a branched glucan with the main chain of (1-->6)-linked alpha-d-glucopyranose residues, approximately 60% of which are 2-substituted with a single alpha-d-glucopyranosyl group. This polysaccharide is a characteristic of various microorganisms, including some lactobacteria studied earlier.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia. zdorovenkoe@mail.ru
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Kachala
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Sidarenka
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Izhik
authors.forename: Elena P
authors.initials: EP
authors.lastname: Kisileva
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Galina I
authors.initials: GI
authors.lastname: Novik
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D001644:Bifidobacterium / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D019936:Probiotics
chemical_list: D011135:Polysaccharides, Bacterial