Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels.
Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived strain A/J, and the Mus musculus castaneus derived strain CAST/Ei. We describe approaches for the accurate identification of nucleotide and structural variation in the genomes of vertebrate experimental organisms, and show how these techniques can be applied to help prioritize candidate genes within quantitative trait loci.
Sudbery
Ian
I
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1HH, UK. ims@sanger.ac.uk
Stalker
Jim
J
Simpson
Jared T
JT
Keane
Thomas
T
Rust
Alistair G
AG
Hurles
Matthew E
ME
Walter
Klaudia
K
Lynch
Dee
D
Teboul
Lydia
L
Brown
Steve D
SD
Li
Heng
H
Ning
Zemin
Z
Nadeau
Joseph H
JH
Croniger
Colleen M
CM
Durbin
Richard
R
Adams
David J
DJ
D000818:Animals; D001483:Base Sequence; D033481:Chromosomes, Mammalian / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics; D018628:Gene Dosage / Q000235:genetics; D016678:Genome / Q000235:genetics; D018095:Germ-Line Mutation / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008285:Major Histocompatibility Complex / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011786:Quality Control; D040641:Quantitative Trait Loci / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D017422:Sequence Analysis, DNA / Q000379:methods*; D017384:Sequence Deletion / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism*
D018388:Codon, Terminator; D012333:RNA, Messenger; D014280:Triglycerides