Identification of neurotoxic chemicals in cell cultures.
In order to identify the neurotoxic potential of drugs or chemicals in vitro, a combination of different in vitro cell culture tests is required. Depending on the type and use of a given test compound, a sequential exposure of freshly isolated and cultured hepatocytes and chicken brain cells is suitable. In order to find out more about the stability of liver-derived metabolites, co-cultures are appropriate. In order to determine how metabolites enter the brain, a combination of an in vitro system which mimics the blood-brain barrier and choroid plexus is proposed.
Bruinink
Arie
A
MaTisMed, Materials-Biology Interactions Lab, EMPA, Materials Science and Technology, St. Gallen, Switzerland. arie.bruinink@empa.ch
Maier
Peter
P
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D002645:Chickens; D018920:Coculture Techniques / Q000379:methods; D003520:Cyclophosphamide / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D009498:Neurotoxins / Q000494:pharmacology*; D051381:Rats
D009498:Neurotoxins; D003520:Cyclophosphamide