title: Platelet-activating factor levels of serum and gingival crevicular fluid in nonsmoking patients with periodontitis and/or coronary heart disease.
abstract: The purpose of the present study was to investigate systemic and local levels of platelet-activating factor (PAF), a potent proinflammatory mediator implicated in cardiovascular pathophysiology in adult nonsmoking patients with periodontitis with or without coronary heart disease (CHD). Eighty-seven volunteers, 25 periodontitis patients, 19 periodontitis with CHD patients, 19 CHD patients, and 24 healthy controls were included, and periodontal conditions were assessed. Gingival crevicular fluid (GCF) and venous blood were collected, and PAF levels were measured by enzyme-linked immunosorbent assay. PAF levels in serum (303.3 ± 204 pg/ml) and in GCF (26.3 ± 6 pg/μl) of the periodontitis group with CHD, the periodontitis group (serum, 302.4 ± 241 pg/ml and GCF, 26.3 ± 8 pg/μl) and the CHD group (serum, 284.7 ± 192 pg/ml and GCF, 20.8 ± 6 pg/μl) were significantly higher than the healthy control group (serum, 65.4 ± 35 pg/ml and GCF, 7.7 ± 3 pg/μl; p < 0.05). In summary, the present study could demonstrate that in patients with periodontitis, the inflammatory mediator PAF is released into serum at least in the same range as for patients with coronary heart disease. However, no additive effects were seen when both conditions were present.
authors.affiliation: Department of Conservative Dentistry and Periodontics, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Pei
authors.initials: P
authors.lastname: Zheng
authors.forename: Haihua
authors.initials: H
authors.lastname: Zhu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Lili
authors.initials: L
authors.lastname: Zhao
authors.forename: Nour Eddine
authors.initials: NE
authors.lastname: El Mokhtari
authors.forename: Jörg
authors.initials: J
authors.lastname: Eberhard
authors.forename: Markus
authors.initials: M
authors.lastname: Lins
authors.forename: Søren
authors.initials: S
authors.lastname: Jepsen
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000097:blood / Q000378:metabolism; D003327:Coronary Disease / Q000097:blood* / Q000150:complications / Q000378:metabolism; D003774:Dental Plaque Index; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005884:Gingival Hemorrhage / Q000097:blood / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000097:blood / Q000378:metabolism; D010514:Periodontal Pocket / Q000097:blood / Q000378:metabolism; D010518:Periodontitis / Q000097:blood* / Q000150:complications / Q000378:metabolism; D010972:Platelet Activating Factor / Q000032:analysis*; D013440:Sulfides / Q000032:analysis; D055815:Young Adult
chemical_list: D018836:Inflammation Mediators; D010972:Platelet Activating Factor; D013440:Sulfides