Effects of urea and trimethylamine-N-oxide on the properties of water and the secondary structure of hen egg white lysozyme.
The influence of urea and trimethylamine-N-oxide (TMAO) on the structure of water and secondary structure of hen egg white lysozyme (HEWL) has been investigated. The hydration of these osmolytes was studied in aqueous solutions by means of FTIR spectra of HDO isotopically diluted in H(2)O. The difference spectra procedure was applied to remove the contribution of bulk water and thus to separate the spectra of solute-affected HDO. The structural-energetic characteristic of these solute-affected water molecules shows that, on average, water affected by TMAO forms stronger H-bonds and is more ordered than pure water. In the case of urea, the H-bonds are very similar to those in pure water. To facilitate the interpretation of the obtained spectral results, calorimetric measurements, DFT calculations, and molecular dynamics (MD) simulations of aqueous osmolyte clusters were performed. All of these results confirmed that the interactions of TMAO with water molecules are much stronger than those of urea with water. Additional ATR FTIR measurements were performed to characterize the influence of the examined osmolytes on the secondary structure of HEW lysozyme. The type of interactions (direct or indirect) was determined, based on the second derivatives of ATR protein spectra record during an increase in the osmolyte concentration. The changes in the amide I band shape caused by urea or TMAO were found to correlate quite well with changes in the water structure around these osmolytes.
Panuszko
Aneta
A
Department of Physical Chemistry, Chemical Faculty, Gdańsk University of Technology, Poland.
Bruździak
Piotr
P
Zielkiewicz
Jan
J
Wyrzykowski
Dariusz
D
Stangret
Janusz
J
D000818:Animals; D002151:Calorimetry; D002645:Chickens; D006860:Hydrogen Bonding; D008744:Methylamines / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D014508:Urea / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D008744:Methylamines; D014867:Water; D014508:Urea; C421864:hen egg lysozyme; D009113:Muramidase; C005855:trimethyloxamine