title: Low-intensity pulsed ultrasound (LIPUS) increases the articular cartilage type II collagen in a rat osteoarthritis model.
abstract: In this study, the effect of low-intensity pulsed ultrasound (LIPUS) on cartilage was evaluated in a rat osteoarthritis (OA) model using serum biomarkers such as CTX-II (type II collagen degradation) and CPII (type II collagen synthesis) as well as histological criteria (Mankin score and immunohistochemical type II collagen staining). OA was surgically induced in the knee joint of rats by anterior cruciate/medial collateral ligament transection and medial meniscus resection (ACLT + MMx). Animals were divided into three groups: sham-operated group (Sham), ACLT + MMx group without LIPUS (-LIPUS), and ACLT + MMx group with LIPUS (+LIPUS; 30 mW/cm(2), 20 min/day for 28 days). CTX-II levels were elevated in both -LIPUS and +LIPUS groups compared to that in the Sham group after the operation, but there was no significant difference between +LIPUS and -LIPUS groups, suggesting that LIPUS does not affect the degradation of type II collagen in this model. In contrast, CPII was significantly increased in +LIPUS group compared to -LIPUS and Sham. Moreover, histological damage on the cartilage (Mankin score) was ameliorated by LIPUS, and type II collagen was immunohistochemically increased by LIPUS in the cartilage of an OA model. Of interest, mRNA expression of type II collagen was enhanced by LIPUS in chondrocytes. Together these observations suggest that LIPUS is likely to increase the type II collagen synthesis in articular cartilage, possibly via the activation of chondrocytes and induction of type II collagen mRNA expression, thereby exhibiting chondroprotective action in a rat OA model.
authors.affiliation: Department of Orthopaedic Surgery, Juntendo University Shizuoka Hospital, 1129 Nagaoka, Izunokuni, Shizuoka 410-2295, Japan. knaito@juntendo.ac.jp
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Naito
authors.forename: Taiji
authors.initials: T
authors.lastname: Watari
authors.forename: Tomoya
authors.initials: T
authors.lastname: Muta
authors.forename: Atsushi
authors.initials: A
authors.lastname: Furuhata
authors.forename: Hideaki
authors.initials: H
authors.lastname: Iwase
authors.forename: Mamoru
authors.initials: M
authors.lastname: Igarashi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kurosawa
authors.forename: Isao
authors.initials: I
authors.lastname: Nagaoka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D024043:Collagen Type II / Q000096:biosynthesis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009300:Nasal Septum / Q000166:cytology / Q000378:metabolism; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014464:Ultrasonic Therapy / Q000379:methods*
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D012333:RNA, Messenger