title: A method to immunolabel rodent spinal cord neurons and glia for molecular study in specific laser microdissected cells involved in neurodegenerative disorders.
abstract: Neuron-glia interaction is involved in physiological function of neurons, however, recent evidences have suggested glial cells as participants in neurotoxic and neurotrophic mechanisms of neurodegenerative/neuroregenerative processes. Laser microdissection offers a unique opportunity to study molecular regulation in specific immunolabeled cell types. However, an adequate protocol to allow morphological and molecular analysis of rodent spinal cord astrocyte, microglia and motoneurons remains a big challenge. In this paper we present a quick method to immunolabel those cells in flash frozen sections to be used in molecular biology analyses after laser microdissection and pressure catapulting.
authors.affiliation: Neuroregeneration Center, Department of Neurology, University of São Paulo School of Medicine, São Paulo, SP 01246-903, Brazil.
authors.forename: Gabriela Pintar
authors.initials: GP
authors.lastname: de Oliveira
authors.forename: Jessica Ruivo
authors.initials: JR
authors.lastname: Maximino
authors.forename: Chin Jia
authors.initials: CJ
authors.lastname: Lin
authors.forename: Gerson
authors.initials: G
authors.lastname: Chadi
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007834:Lasers; D008297:Male; D051379:Mice; D042282:Microdissection; D019636:Neurodegenerative Diseases / Q000473:pathology*; D009457:Neuroglia / Q000473:pathology*; D009474:Neurons / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*