[Screening and breeding of high lysine-producing strains by genome shuffling].
OBJECTIVE
To screen and breed high lysine-producing strains by genome shuffling.


METHODS
Corynebacterium pekinense 1 was used as the starting strain in this study. High lysine-producing strains were screened by genome shuffling.


RESULTS
Five hereditarily stable strains with high lysine production were obtained by four cycles of genome shuffling. A high lysine producing strain, F4-36, was obtained, which produced 16.95 g/dL lysine, 37.1% higher than that of the starting strain.


CONCLUSIONS
Genome shuffling can be an efficient tool to screen and breed high lysine production strains.
Zhao
Kai
K
Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China. zk395@yahoo.com.cn
Duan
Wei
W
Sun
Lixin
L
Zhou
Dongpo
D
D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism*; D032621:DNA Shuffling; D016680:Genome, Bacterial; D008239:Lysine / Q000378:metabolism*
D008239:Lysine