[Immunogenecity of cytomegalovirus-infected fibroblasts].
OBJECTIVE
To analyze the capability of cytomegalovirus (CMV)-infected human embryonic lung fibroblasts (HELFs) to induce immune response.


METHODS
HELFs were infected with cytomegalovirus and stained with antibody against HLA-A2 molecular, the expression of HLA-A2 was detected by FCM. The infected HELFs were incubated with individual pp65 peptide NLVPMVATV. While the uninfected and unloaded infected HELFs served as control respectively. After PBMC was added to the differently treated HELFs and incubated, the immune response was measured with IFN-gamma release as readout.


RESULTS
The expression of HLA-A molecular on infected fibroblasts diminished markedly compared with that on the uninfected. The peptides expressed on the infected HELFs together with those pulsed externally induced a stronger response than the infected HELFs alone.


CONCLUSIONS
Although CMV can down-regulate the expression of MHC I on the infected cells, it can not decrease the capacity of cells to present peptides loaded externally, and therefore still induce immune response to some extent.
Wang
Yan-Jun
YJ
Institute for Hepatitis, Beijing You-an Hospital, Capital Medical University, Beijing 100069, China. yjunwang@ccmu.edu.cn
Sun
Bin
B
Liu
Dao-Jie
DJ
Wu
Yan-Ning
YN
Zhao
Jing-Jing
JJ
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003587:Cytomegalovirus / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology; D015234:HLA-A Antigens / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D010750:Phosphoproteins / Q000737:chemistry; D014763:Viral Matrix Proteins / Q000737:chemistry
D015234:HLA-A Antigens; D010446:Peptide Fragments; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa