Regulatory activity of heterologous gene-activator xlnR of Aspergillus niger in Penicillium canescens.
The gene encoding the xlnR xylanolytic activator of the heterologous fungus Aspergillus niger was incorporated into the Penicillium canescens genome. Integration of the xlnR gene resulted in the increase in a number of activities, i.e. endoxylanase, beta-xylosidase, alpha-L-arabinofuranosidase, alpha-galactosidase, and feruloyl esterase, compared to the host P. canescens PCA 10 strain, while beta-galactosidase, beta-glucosidase, endoglucanase, and CMCase activities remained constant. Two different expression constructs were developed. The first consisted of the nucleotide sequence containing the mature P. canescens phytase gene under control of the axhA promoter region gene encoding A. niger (1,4)-beta-D-arabinoxylan-arabinofuranohydrolase. The second construct combined the P. canescens phytase gene and the bgaS promoter region encoding homologous beta-galactosidase. Both expression cassettes were transformed into P. canescens host strain containing xlnR. Phytase synthesis was observed only for strains with the bgaS promoter on arabinose-containing culture media. In conclusion, the bgaS and axhA promoters were regulated by different inducers and activators in the P. canescens strain containing a structural tandem of the axhA promoter and the gene of the xlnR xylanolytic activator.
Vinetsky
Y P
YP
Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia. vinetski@genetika.ru
Rozhkova
A M
AM
Chulkin
A M
AM
Satrutdinov
A D
AD
Sinitsyna
O A
OA
Fedorova
E A
EA
Bekkarevich
A O
AO
Okunev
O N
ON
Sinitsyn
A P
AP
D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator; D005818:Genetic Engineering; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D000519:alpha-Galactosidase / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
D005656:Fungal Proteins; D015534:Trans-Activators; C115443:XlnR protein, Aspergillus; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D000519:alpha-Galactosidase; D001616:beta-Galactosidase; D002480:Cellulase; C019826:carboxymethylcellulase; C019713:alpha-N-arabinofuranosidase