title: Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies.
abstract: OBJECTIVE
  The aim of this study was to investigate the levels of cytokines in placenta-derived mesenchymal stem cells (MSCs) in normal pregnancies and those with pre-eclampsia.
  
  
  METHODS
  C5a, CD40 Ligand, G-CSF, GM-CSF, GROalpha, I-309, sICAM-1, IFN-gamma, IL-1alpha, IL-1beta, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-16, IL-17, IL-17E, IL-23, IL-27, IL-32alpha, IP-10, I-TAC, MCP-1, MIF, MIP-1alpha, MIP-1beta, Serpin E1, RANTES, SDF-1, TNFalpha, and sTREM-1 were measured in mesenchymal stem cells using the human cytokine array panel A. The soluble intracellular adhesion molecule-1 (sICAM-1), stromal-derived factor-1 (SDF-1) and monocyte chemotactic protein-1 (MCP-1) were measured by real-time PCR and confirmed by Western blot analysis.
  
  
  RESULTS
  MSCs derived from the deciduas of normal pregnancies had significantly elevated levels of sICAM (p=0.000) and SDF-1 (p=0.011), compared to the pregnancies with pre-eclampsia. The level of MCP-1 in the decidua-derived MSCs was not significantly different. No significant difference was observed between normal and pre-eclamptic pregnancies for the amnion-derived MSCs.
  
  
  CONCLUSIONS
  The decreased levels of sICAM and SDF-1 found in the decidua-derived MSCs from pre-eclamptic pregnancies might be associated with some of the immunological alterations in pre-eclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jong Ha
authors.initials: JH
authors.lastname: Hwang
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Lee
authors.forename: Oye Sun
authors.initials: OS
authors.lastname: Seok
authors.forename: Yu Chin
authors.initials: YC
authors.lastname: Paek
authors.forename: Geum Joon
authors.initials: GJ
authors.lastname: Cho
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Seol
authors.forename: Jae Kwan
authors.initials: JK
authors.lastname: Lee
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Oh
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006801:Humans; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D010920:Placenta / Q000166:cytology* / Q000276:immunology; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy / Q000276:immunology*
chemical_list: D018932:Chemokine CCL2; D054377:Chemokine CXCL12; D016207:Cytokines; D018799:Intercellular Adhesion Molecule-1