[Construction of an infectious cDNA clone derived from foot-and-mouth disease virus O/QYYS/s/06].
After sequencing, we amplified and cloned foot-and-mouth disease virus (FMDV) O/QYYS/s/06 whole genome by three fragments. These three fragments were cloned into vector P43 one by one to construct recombinant plasmid P43C, which carried the full-length cDNA of FMDV O/QYYS/s/06. Then, plasmid P43C and plasmid T7 expressing T7 RNA polymerase were co-transfected into BHK-21 cells. After 48 h, we harvested the culture broth from transfected BHK-21 cells and inoculated into 2-3 day-old sucking mice. After four generation passage, the virus harvested from sucking mice was confirmed to be type O FMDV by the indirect hemagglutination test, sucking mice's neutralization test and sequencing. The results showed that we have successfully constructed the full-length cDNA clone of FMDV O/QYYS/s/06 strain.
Lu
Shousheng
S
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Zhao
Qizu
Q
Liu
Xiangtao
X
Sun
Yanwei
Y
Ren
Tao
T
Zhang
Guihong
G
Qi
Wenbao
W
Zha
Yunfeng
Y
Kong
Lingchen
L
Zhang
Han
H
Fan
Huiying
H
Liao
Ming
M
D000818:Animals; D000831:Animals, Newborn; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D005536:Foot-and-Mouth Disease / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D051379:Mice; D014158:Transcription, Genetic; D014162:Transfection
D018076:DNA, Complementary; D004279:DNA, Viral