title: Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity.
abstract: OBJECTIVE
  To observe the effects of traditional antiinflammatory medicine Lonicerae Flos (LF) on rat reflux esophagitis (RE) induced by pylorus and forestomach ligation compared with the well-known proton antioxidant, alpha-tocopherol.
  
  
  METHODS
  Rats were pretreated with three different dosages of LF (500, 250 and 125 mg/kg) orally, once a day for 14 d before pylorus and forestomach ligation. Nine hours after pylorus and forestomach ligation, changes to the stomach and esophagus lesion areas, gastric volumes, acid and pepsin outputs, antioxidant effects, esophageal lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), myeloperoxidase and glutathione (GSH) levels, and collagen contents (marker of flexibility) were observed on the esophageal and fundic histopathology. The results were compared with an alpha-tocopherol (once orally, 1 h before operation, 30 mg/kg) treated group in which the effects on RE were already confirmed.
  
  
  RESULTS
  Pylorus and forestomach ligations caused marked increases of gross esophageal and gastric mucosa lesion areas, which corresponded with histopathological changes. In addition, increases of esophageal lipid peroxidation, decreases of SOD, CAT, and GSH-free radical scavengers, increases of collagen were observed. However, these pylorus and forestomach ligation induced RE were dose-dependently inhibited by treatment of 500, 250 and 125 mg/kg of LF extract, mediated by antioxidant effects. RE at 250 mg/kg showed similar effects alpha-tocopherol.
  
  
  CONCLUSIONS
  The results suggest that antioxidant effects of LF could attenuate the severity of RE and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease.
authors.affiliation: Department of Histology and Anatomy, College of Oriental Medicine, Daegu Haany University, Gyeongsan, South Korea.
authors.forename: Sae-Kwang
authors.initials: SK
authors.lastname: Ku
authors.forename: Bu-Il
authors.initials: BI
authors.lastname: Seo
authors.forename: Ji-Ha
authors.initials: JH
authors.lastname: Park
authors.forename: Gyu-Yeol
authors.initials: GY
authors.lastname: Park
authors.forename: Young-Bae
authors.initials: YB
authors.lastname: Seo
authors.forename: Jae-Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyeung-Sik
authors.initials: HS
authors.lastname: Lee
authors.forename: Seong-Soo
authors.initials: SS
authors.lastname: Roh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004942:Esophagitis, Peptic / Q000188:drug therapy*; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D031239:Lonicera / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D011708:Pylorus / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000187:drug effects*; D024502:alpha-Tocopherol / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D005978:Glutathione; D024502:alpha-Tocopherol