Increased fetal cell trafficking in murine lung following complete pregnancy loss from exposure to lipopolysaccharide.
To determine whether chemically induced miscarriage affects fetomaternal trafficking in a mouse model, we measured the amount of fetal DNA present in various maternal organs by polymerase chain reaction amplification following exposure to lipopolysaccharide (LPS). As the frequency of fetal cells and the number of animals with detectable microchimerism following LPS injection were significantly increased, particularly in lung tissue compared to controls, with no signs of an inflammatory response, we conclude that LPS-induced miscarriage results in increased murine fetomaternal cell trafficking, supporting a relationship between fetal loss and the establishment of fetal cell microchimerism.
Johnson
Kirby L
KL
Division of Genetics, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, 800 Washington Street, Box 394, Boston, MA 02111, USA. kjohnson@tuftsmedicalcenter.org
Tao
Kai
K
Stroh
Helene
H
Kallenbach
Lisa
L
Peter
Inga
I
Richey
Lauren
L
Rust
Daniel
D
Bianchi
Diana W
DW
D000022:Abortion, Spontaneous / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D000818:Animals; D002465:Cell Movement; D046528:Chimerism; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus / Q000378:metabolism / Q000473:pathology*; D005865:Gestational Age; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D011247:Pregnancy
D008070:Lipopolysaccharides; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins