Antitumor effects of a recombinant pseudotype baculovirus expressing Apoptin in vitro and in vivo.
Apoptin, a chicken anemia virus-derived, p53-independent, bcl-2-insenstive apoptotic protein with the ability to specifically induce apoptosis in tumor or transformed cells, is a promising tool for cancer gene therapy. In this study, pseudotype baculovirus, a recently developed alternative gene delivery system, was used as a vector to express Apoptin. The resultant recombinant baculovirus (BV-Apoptin) efficiently expressed the Apoptin protein and induced apoptosis in HepG2 and H22 cells. Studies in vivo showed that intratumoral injection of BV-Apoptin into a xenogeneic tumor (derived from H22 murine hepatoma cells in C57BL/6 mice) significantly suppressed tumor growth, and significantly prolonged the survival of tumor-bearing mice compared to a control pseudotype baculovirus that expressed EGFP. Taken together, these results suggest that Apoptin, expressed from the pseudotype baculovirus vector, has the potential to become a therapeutic agent for the treatment of solid tumors.
Pan
Yongfei
Y
Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
Fang
Liurong
L
Fan
Huiying
H
Luo
Rui
R
Zhao
Qian
Q
Chen
Huanchun
H
Xiao
Shaobo
S
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics* / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002460:Cell Line; D045744:Cell Line, Tumor; D017727:Chicken anemia virus / Q000235:genetics; D005434:Flow Cytometry; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney; D008114:Liver Neoplasms, Experimental / Q000401:mortality / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D015996:Survival Rate
D036022:Capsid Proteins; C091717:VP3 protein, Chicken anemia virus