title: Performance levels of four Latin American laboratories for the serodiagnosis of Chagas disease in Mexican sera samples.
abstract: In nearly all of the previous multicentre studies evaluating serological tests for Trypanosoma cruzi infection, sera samples from Central or South American countries have been used preferentially. In this work we compared the reliability of the serological tests using Mexican sera samples that were evaluated in four independent laboratories. This included a reference laboratory in Brazil and three participant laboratories, including one in Central America and two in Mexico. The kappa index between Brazilian and Honduran laboratories reached 1.0 and the index for the Mexican laboratories reached 0.94. Another finding of this study was that the source of antigen did not affect the performance of the serological tests.
authors.affiliation: Instituto de Patología Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania, Brazil.
authors.forename: Alejandro O
authors.initials: AO
authors.lastname: Luquetti
authors.forename: Bertha
authors.initials: B
authors.lastname: Espinoza
authors.forename: Ignacio
authors.initials: I
authors.lastname: Martínez
authors.forename: Nidia
authors.initials: N
authors.lastname: Hernández-Becerril
authors.forename: Carlos
authors.initials: C
authors.lastname: Ponce
authors.forename: Elisa
authors.initials: E
authors.lastname: Ponce
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Reyes
authors.forename: Oscar
authors.initials: O
authors.lastname: Hernández
authors.forename: Ruth
authors.initials: R
authors.lastname: López
authors.forename: Victor
authors.initials: V
authors.lastname: Monteón
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D001938:Brazil; D014355:Chagas Disease / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006721:Honduras; D006801:Humans; D007753:Laboratories / Q000592:standards*; D008800:Mexico; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards*; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan