In vitro selection of RNA aptamers to a protein target by filter immobilization.
This unit describes the selection of aptamers from a pool of single-stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially act as protein function inhibitors, and may find applications as therapeutic or diagnostic reagents. A pool of dsDNA is used to generate an ssRNA pool, which is mixed with the protein target. Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription.
Hall
Bradley
B
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA.
Arshad
Seyed
S
Seo
Kyunghyun
K
Bowman
Catherine
C
Corley
Meredith
M
Jhaveri
Sulay D
SD
Ellington
Andrew D
AD
D052157:Aptamers, Nucleotide / Q000302:isolation & purification* / Q000378:metabolism*; D019020:Directed Molecular Evolution; D008967:Molecular Biology / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D052156:SELEX Aptamer Technique / Q000379:methods*
D052157:Aptamers, Nucleotide; D011506:Proteins