title: Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks.
abstract: Mrc1 (mediator of replication checkpoint), Tof1 (topoisomerase I interacting factor), and Csm3 (chromosome segregation in meiosis) are checkpoint-mediator proteins that function during DNA replication and activate the effector kinase Rad53. We reported previously that Mrc1 and Tof1 are constituents of the replication machinery and that both proteins are required for the proper arrest and stabilization of replication forks in the presence of hydroxyurea. In our current study, we show that Csm3 is a component of moving replication forks and that both Tof1 and Csm3 are specifically required for the association of Mrc1 with these structures. In contrast, the deletion of mrc1 did not affect the association of Tof1 and Csm3 with the replication fork complex. In agreement with previous observations in yeast cells, the results of a baculovirus coexpression system showed that these three proteins interact directly with each other to form a mediator complex in the absence of replication forks.
authors.affiliation: Department of Biological Science, Laboratory of Chromosome Structure and Function, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B-20, 4259, Nagatsuta, Midori-ku, Yokohama City, Kanagawa, 226-8501, Japan.
authors.forename: Masashige
authors.initials: M
authors.lastname: Bando
authors.forename: Yuki
authors.initials: Y
authors.lastname: Katou
authors.forename: Makiko
authors.initials: M
authors.lastname: Komata
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Takehiko
authors.initials: T
authors.lastname: Itoh
authors.forename: Takashi
authors.initials: T
authors.lastname: Sutani
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shirahige
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D064447:Checkpoint Kinase 2; D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006918:Hydroxyurea / Q000737:chemistry; D007313:Insecta; D008957:Models, Genetic; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D018797:Cell Cycle Proteins; C508610:Csm3p protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; C441579:MRC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C119636:TOF1 protein, S cerevisiae; D064447:Checkpoint Kinase 2; D017346:Protein Serine-Threonine Kinases; C067022:RAD53 protein, S cerevisiae; D006918:Hydroxyurea