title: Carotenogenesis in Haematococcus lacustris: role of protein tyrosine phosphatases.
abstract: In the present study, we examined the inhibitory effects of protein tyrosine phosphatase (PTPase) inhibitors, including sodium orthovanadate (SOV), ammonium molybdate (AM), and iodoacetamide (IA), on cell growth, accumulation of astaxanthin, and PTPase activity in the photosynthetic algae Haematococcus lacustris. PTPase activity was assayed spectrophotometrically and was found to be inhibited 60% to 90% after treatment with the inhibitors. SOV markedly abolished PTPase activity, significantly activating the accumulation of astaxanthin. These data suggest that the accumulation of astaxanthin in H. lacustris results from the concerted actions of several PTPases.
authors.affiliation: Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
authors.forename: Jae-Kweon
authors.initials: JK
authors.lastname: Park
authors.forename: Phuong Ngoc
authors.initials: PN
authors.lastname: Tran
authors.forename: Jeong-Dong
authors.initials: JD
authors.lastname: Kim
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Hong
authors.forename: Choul-Gyun
authors.initials: CG
authors.lastname: Lee
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D056890:Eukaryota / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007460:Iodoacetamide / Q000494:pharmacology; D008982:Molybdenum / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D002338:Carotenoids; D014638:Vanadates; D008982:Molybdenum; D017027:Protein Tyrosine Phosphatases; C022175:ammonium molybdate; D007460:Iodoacetamide