Highly selective detection of Cu2+ utilizing specific binding between Cu-demetallated superoxide dismutase 1 and the Cu2+ ion via surface plasmon resonance spectroscopy.
A highly specific interaction between a metal-deficient metalloenzyme and metal ion has been utilized in the selective detection of the metal ion by surface plasmon resonance spectroscopy (SPRS). The use of SPRS and Cu-demetallated superoxide dismutase (E,Zn-SOD1) as a sensing actuator allows one to selectively and in situ detect Cu2+ without any interference that other spectroscopic methods may have.
Lee
Suseung
S
School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 151-744, Korea.
Choi
Inhee
I
Hong
Surin
S
In Yang
Young
Y
Lee
Jeongjin
J
Kang
Taewook
T
Yi
Jongheop
J
D002412:Cations / Q000032:analysis; D003300:Copper / Q000032:analysis*; D006801:Humans; D008958:Models, Molecular; D012680:Sensitivity and Specificity; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*
D002412:Cations; D003300:Copper; D013482:Superoxide Dismutase