Molecular epidemiology of Brazilian pseudorabies viral isolates.
Pseudorabies is a disease caused by pseudorabies virus (PRV) and is responsible for considerable economic losses in the swine industry. The objective of this work was to use molecular epidemiology as a tool to facilitate the study of PRV outbreaks in Brazil. The standard PRV strain Shope, the vaccine strain Bartha and isolates from the south and the southeast regions of Brazil, were amplified for gE and gC partial genes by PCR. Results indicated that Brazilian PRV isolates are grouped in two clusters, A and B, except for one isolate that grouped with Bartha and Shope. Most Brazilian PRV isolates belonged to cluster B and diverged from virus isolated from other countries.
Fonseca
Antônio Augusto
AA
Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG, Brazil. augustofj@yahoo.com.br
Camargos
Marcelo Fernandes
MF
de Oliveira
Anapolino Macedo
AM
Ciacci-Zanella
Janice R
JR
Patrício
Maria Aparecida C
MA
Braga
Alexandre C
AC
Cunha
Eliane S
ES
D'Ambros
Régia
R
Heinemann
Marcos Bryan
MB
Leite
Rômulo Cerqueira
RC
dos Reis
Jenner K Pimenta
JK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011557:Pseudorabies / Q000453:epidemiology* / Q000821:virology*; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1; C038535:glycoprotein gC, herpes simplex virus type 1