The anti-inflammatory effect of baicalin on hypoxia/reoxygenation and TNF-alpha induced injury in cultural rat cardiomyocytes.
The aim of present study was to investigate the effect of baicalin on hypoxia/reoxygenation (H/R) injury in cardiomyocytes and the mechanisms involved, particularly in relation to cytokines. The cardiomyocytes for the H/R groups were placed into a hypoxic chamber for 12 h and then underwent reoxygenation for 1 h. The cells in the TNF-alpha groups were administered 100 ng/mL rrTNF-alpha and incubated for 13 h under normal conditions. The cells in the baicalin pretreatment groups were administered 10 microM baicalin 30 min prior to exposure to H/R or TNF-alpha. It was observed that pretreatment with baicalin (10 microM) significantly reduced the cell damage and death induced by H/R or TNF-alpha. In the culture medium of the H/R cells, the SOD activity increased, while TNF-alpha was decreased by baicalin. The levels of IL-6 in culture medium for H/R or TNF-alpha treated cells were suppressed by baicalin pretreatment. In contrast, the levels of IL-10 in culture medium for H/R or TNF-alpha treated cells were significantly elevated by baicalin. Moreover, baicalin inhibited the nuclear translocation of NF-kappaB induced by H/R or TNF-alpha. In conclusion, baicalin may protect cardiomyocytes from H/R injury through an anti-inflammatory mechanism.
Lin
Lin
L
Department of Pharmacology, School of Basic Medicine, Southeast University, Nanjing 210009, PR China.
Wu
Xiao-dong
XD
Davey
Andrew K
AK
Wang
Jiping
J
D000818:Animals; D015687:Cell Hypoxia; D002470:Cell Survival; D002478:Cells, Cultured; D005419:Flavonoids / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000648:ultrastructure; D016328:NF-kappa B / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000009:adverse effects*
D005419:Flavonoids; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C038044:baicalin; D013482:Superoxide Dismutase; D010100:Oxygen