[Small interfering RNAs targeting human myostatin].
Ten human myostatin small interfering RNAs which sequence was found by two different software products were synthesized and tested for activity. It was found that three of them have pronounced biological activity and decrease myostatin mRNA level to 22-27% of control value. These small interfering RNAs stimulate human myoblast proliferation and decrease their differentiation reliably. The obtained small interfering RNAs can be used for development of new approaches for the treatment of sarcopenia and different myodystrophies.
Furalev
V A
VA
Kravchenko
I V
IV
Popov
V O
VO
D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D055435:Myostatin / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000096:biosynthesis* / Q000235:genetics
C525911:MSTN protein, human; D055435:Myostatin; D034741:RNA, Small Interfering