Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart.
Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have tested published approaches and challenged their practicality. As a result, we propose an improved and streamlined protocol, which consistently yields high-quality histology, thereby minimizing experimental noise and group sizes.
Bohl
Steffen
S
Department of Cardiovascular Medicine, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom. sbohl@well.ox.ac.uk
Medway
Debra J
DJ
Schulz-Menger
Jeanette
J
Schneider
Jurgen E
JE
Neubauer
Stefan
S
Lygate
Craig A
CA
D000818:Animals; D004396:Coloring Agents; D004195:Disease Models, Animal; D007090:Image Interpretation, Computer-Assisted; D007211:Indoles; D051379:Mice; D009203:Myocardial Infarction / Q000473:pathology*; D015428:Myocardial Reperfusion Injury / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D009942:Organometallic Compounds; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; D007211:Indoles; D009942:Organometallic Compounds; C015445:copper phthalocyanine