title: Phylogenetic classification of human papillomavirus genotypes in high-grade cervical intraepithelial neoplasia in women from a densely populated Brazilian urban region.
abstract: OBJECTIVE
  Differences in human papillomavirus (HPV) types may correlate with the biological potential and invasion risk of high-grade cervical intraepithelial neoplasia (CIN 2 and CIN 3). The objective of this study was to determine the relationship between different combinations of HPV types and CIN severity.
  
  
  METHODS
  Cross-sectional study, at Universidade Estadual de Campinas (Unicamp).
  
  
  METHODS
  Cervical samples from 106 women treated due to CIN 2 (18) or CIN 3 (88) were examined for specific HPV genotypes using Roche Linear Array (LA-HPV). The proportions of CIN 2 and CIN 3 in groups of women infected with the HPV phylogenetic groups A7 and A9 were compared. Three groups were formed: women with single infections; multiple infections; and the whole sample.
  
  
  RESULTS
  Multiple infections were detected in 68 samples (64.7%). The most frequent high-risk genotypes detected (single/multiple) were HPV 16 (57.1%), HPV 58 (24.7%), HPV 33 (15.2%), HPV 52 (13.3%), HPV 31 (10.4%), HPV 51 (7.6%) and HPV 18 (6.6%). Women without infection with HPV species Alpha 9 were less likely to have CIN 3 than were their Alpha 9 HPV-infected counterparts. HPV 16 and/or HPV 18, with or without associations with other viral types, were more frequently found in women with CIN 3 than in those with CIN 2.
  
  
  CONCLUSIONS
  The severity of high-grade CIN may be aggravated by the presence of HPV types included in the Alpha 9 phylogenetic classification and by infections including HPV 16 and 18, singly or in combination with other HPV genotypes.
authors.affiliation: Department of Obstetrics and Gynecology, Universidade Estadual de Campinas (Unicamp), São Paulo, Brazil.
authors.forename: Denise Rocha
authors.initials: DR
authors.lastname: Pitta
authors.forename: Luis Otávio
authors.initials: LO
authors.lastname: Sarian
authors.forename: Elisabete Aparecida
authors.initials: EA
authors.lastname: Campos
authors.forename: Sílvia Helena
authors.initials: SH
authors.lastname: Rabelo-Santos
authors.forename: Kari
authors.initials: K
authors.lastname: Syrjänen
authors.forename: Sophie Françoise
authors.initials: SF
authors.lastname: Derchain
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil; D004279:DNA, Viral / Q000235:genetics; D004812:Epidemiologic Methods; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D014505:Urban Population; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000821:virology*
chemical_list: D004279:DNA, Viral