Hydrolytic activity of histidine-containing octapeptides in water identified by quantitative screening of a combinatorial library.
A quantitative on-bead screening of a combinatorial octapeptide library revealed catalysts with hydrolytic activity in water. Histidine is an essential amino acid but the catalytic activity as well as the substrate binding affinity is also dependent on the sequence of the octapeptide.
Schmuck
Carsten
C
Institute of Organic Chemistry, University of Duisburg-Essen, Universitätsstrasse 7, 45141 Essen, Germany. carsten.schmuck@uni-due.de
Michels
Ute
U
Dudaczek
Jürgen
J
D000595:Amino Acid Sequence; D002021:Buffers; D020650:Combinatorial Chemistry Techniques; D004950:Esterases / Q000378:metabolism; D006639:Histidine; D006868:Hydrolysis; D009842:Oligopeptides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D014867:Water
D002021:Buffers; D009842:Oligopeptides; D014867:Water; D006639:Histidine; D004950:Esterases