Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development.
Common lymphoid progenitors (CLPs) clonally produce both B- and T-cell lineages, but have little myeloid potential in vivo. However, some studies claim that the upstream lymphoid-primed multipotent progenitor (LMPP) is the thymic seeding population, and suggest that CLPs are primarily B-cell-restricted. To identify surface proteins that distinguish functional CLPs from B-cell progenitors, we used a new computational method of Mining Developmentally Regulated Genes (MiDReG). We identified Ly6d, which divides CLPs into two distinct populations: one that retains full in vivo lymphoid potential and produces more thymocytes at early timepoints than LMPP, and another that behaves essentially as a B-cell progenitor.
Inlay
Matthew A
MA
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA. minlay@stanford.edu
Bhattacharya
Deepta
D
Sahoo
Debashis
D
Serwold
Thomas
T
Seita
Jun
J
Karsunky
Holger
H
Plevritis
Sylvia K
SK
Dill
David L
DL
Weissman
Irving L
IL
D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental; D054503:Lymphoid Progenitor Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*
D000950:Antigens, Ly; D058851:GPI-Linked Proteins; C035271:Ly6d protein, mouse