Rapid LC-MS detection of cyanobacterial hepatotoxins microcystins and nodularins--comparison of columns.
Eight reversed-phase columns intended for rapid HPLC were assessed for the separation of thirteen microcystins and nodularins, cyclic peptidic hepatotoxins. The instrumentation consisted of an Agilent Technologies 1200 Rapid Resolution high performance liquid chromatography system coupled to a mass spectrometer, Bruker Daltonics Ultra Performance High Capacity Ion Trap MS (HCT Ultra) with electrospray ionisation (RRLC-ESI-IT-MS). The columns tested were 2-2.1 mm x 50 mm in diameter and length, and contained small particles (1.8-2.7 microm), or monolithic silica supports for fast performance. The shortest total run time achieved was 3 min 15 s including equilibration and injection. Critical microcystin pairs were still resolved. Several columns showed excellent performance.
Neffling
Milla-Riina
MR
Department of Biochemistry and Pharmacy, Abo Akademi University, Tykistökatu 6 A, Biocity 3rd floor, FI-20520 Turku, Finland. mneffling@gmail.com
Spoof
Lisa
L
Meriluoto
Jussi
J
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D052998:Microcystins / Q000032:analysis*; D046931:Microcystis / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000032:analysis*; D013499:Surface Properties
D052998:Microcystins; D010456:Peptides, Cyclic; C063998:nodularin