title: MicroRNA expression profiling of megakaryocytes in primary myelofibrosis and essential thrombocythemia.
abstract: In primary myelofibrosis (PMF) and essential thrombocythemia (ET) the megakaryocytic lineage characteristically shows aberrant proliferation and maturation in which the regulatory microRNA (miR) system might be involved. Laser-microdissected PMF and ET megakaryocytes were analysed with real-time polymerase chain reaction (PCR) low density arrays comprising 365 microRNAs. The highest megakaryocytic expression levels were observed for miR-223, which is known to be expressed also in granulopoiesis. Cluster analysis revealed a tendency of disease-specific megakaryocytic microRNA expression profiles indicating that a complex shift of microRNA expression appears to be the underlying defect. Increased accumulation of miR-146b was observed in cellular stage PMF (p = 0.0125) but not ET megakaryopoiesis. In conclusion, this is the first microRNA profiling of in situ-derived PMF, ET and normal megakaryocytes.
authors.affiliation: Institute of Pathology, 30625, Hannover, Germany. Hussein.Kais@MH-Hannover.de
authors.forename: Kais
authors.initials: K
authors.lastname: Hussein
authors.forename: Katharina
authors.initials: K
authors.lastname: Theophile
authors.forename: Wiebke
authors.initials: W
authors.lastname: Dralle
authors.forename: Birgitt
authors.initials: B
authors.lastname: Wiese
authors.forename: Hans
authors.initials: H
authors.lastname: Kreipe
authors.forename: Oliver
authors.initials: O
authors.lastname: Bock
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D002648:Child; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D055728:Primary Myelofibrosis / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013920:Thrombocythemia, Essential / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: D035683:MicroRNAs