title: Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development.
abstract: Common lymphoid progenitors (CLPs) clonally produce both B- and T-cell lineages, but have little myeloid potential in vivo. However, some studies claim that the upstream lymphoid-primed multipotent progenitor (LMPP) is the thymic seeding population, and suggest that CLPs are primarily B-cell-restricted. To identify surface proteins that distinguish functional CLPs from B-cell progenitors, we used a new computational method of Mining Developmentally Regulated Genes (MiDReG). We identified Ly6d, which divides CLPs into two distinct populations: one that retains full in vivo lymphoid potential and produces more thymocytes at early timepoints than LMPP, and another that behaves essentially as a B-cell progenitor.
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA. minlay@stanford.edu
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Inlay
authors.forename: Deepta
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Debashis
authors.initials: D
authors.lastname: Sahoo
authors.forename: Thomas
authors.initials: T
authors.lastname: Serwold
authors.forename: Jun
authors.initials: J
authors.lastname: Seita
authors.forename: Holger
authors.initials: H
authors.lastname: Karsunky
authors.forename: Sylvia K
authors.initials: SK
authors.lastname: Plevritis
authors.forename: David L
authors.initials: DL
authors.lastname: Dill
authors.forename: Irving L
authors.initials: IL
authors.lastname: Weissman
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental; D054503:Lymphoid Progenitor Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*
chemical_list: D000950:Antigens, Ly; D058851:GPI-Linked Proteins; C035271:Ly6d protein, mouse