Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum.
To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay's sensitivity and specificity were 78.2 and 94% respectively.
Moyano
Eva M
EM
Centro Nacional de Medicina Tropical, Instituto de Salud Carlos III, Sinesio Delgado, 6; Pabellón 13, 28020 Madrid, Spain.
González
Luis Miguel
LM
Cuevas
Laureano
L
Perez-Pastrana
Esperanza
E
Santa-Maria
Ysmael
Y
Benito
Agustín
A
D000595:Amino Acid Sequence; D000953:Antigens, Protozoan / Q000276:immunology; D015153:Blotting, Western; D002457:Cell Extracts; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology / Q000648:ultrastructure; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment
D000953:Antigens, Protozoan; D002457:Cell Extracts; D018076:DNA, Complementary; D005819:Genetic Markers; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C050256:ring-infected erythrocyte surface antigen (RESA), Plasmodium falciparum