title: Detection of early precursors of t(12;21) positive pediatric acute lymphoblastic leukemia during follow-up.
abstract: DNA-, RNA-, and cell-based methods provide different biologic information for determining the presence of minimal residual disease (MRD). We monitored the responses of patients with pediatric acute lymphoblastic leukemia (pALL) using DNA markers, TEL/AML1 expression, and scanning fluorescence microscopy (SFM). Using SFM, 36% of patients exhibited 1.5-3.1 log and 2.9-4.2 log higher MRD levels compared with those based on DNA and RNA markers, respectively. CD10+ ancestor cells with germline antigen receptors, but silent TEL/AML1 expression, may reside in the lymphoid stem cell compartment of treated t(12;21)-positive patients and might act as a potential source of cells for late relapses.
authors.affiliation: Department of Pathology, University of Pécs Medical Center, Pécs, Hungary. laszlo.pajor@kk.pte.hu
authors.forename: Renáta
authors.initials: R
authors.lastname: László
authors.forename: Donát
authors.initials: D
authors.lastname: Alpár
authors.forename: Béla
authors.initials: B
authors.lastname: Kajtár
authors.forename: Agnes
authors.initials: A
authors.lastname: Lacza
authors.forename: Gábor
authors.initials: G
authors.lastname: Ottóffy
authors.forename: Csongor
authors.initials: C
authors.lastname: Kiss
authors.forename: Katalin
authors.initials: K
authors.lastname: Bartyik
authors.forename: Kálmán
authors.initials: K
authors.lastname: Nagy
authors.forename: László
authors.initials: L
authors.lastname: Pajor
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005500:Follow-Up Studies; D006801:Humans; D018365:Neoplasm, Residual / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics; D014178:Translocation, Genetic; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D004273:DNA, Neoplasm; D015514:Oncogene Proteins, Fusion; D012334:RNA, Neoplasm; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein