title: Pyrene-labeled deoxyguanosine as a fluorescence sensor to discriminate single and double stranded DNA structures: design of ends free molecular beacons.
abstract: A novel fluorescent DNA probe containing pyrene-labeled C8 alkylamino-substituted 2'-deoxyguanosine was designed in order to discriminate single stranded and double stranded regions in DNA. This fluorescent sensor was used for the design of practically useful 3'- and 5'-ends free self-quenched molecular beacon (MB). Unique MB detectable by pyrene excimer fluorescence was also demonstrated.
authors.affiliation: Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yuta
authors.initials: Y
authors.lastname: Shinohara
authors.forename: Subhendu S
authors.initials: SS
authors.lastname: Bag
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D002273:Carcinogens / Q000633:toxicity; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D005453:Fluorescence; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D002273:Carcinogens; D015342:DNA Probes; D011721:Pyrenes; D004247:DNA