Fabrication of chronocoulometric DNA sensor based on gold nanoparticles/poly(l-lysine) modified glassy carbon electrode.
In this work, we fabricated a sensitivity chronocoulometric DNA sensor (CDS) based on gold nanoparticles (AuNPs)/poly(L-lysine) complex film modified glassy carbon electrode. Hexaammineruthenium(III) chloride ([Ru(NH3)6]3+) was used as the electroactive indicator. The assembled process was investigated by cyclic voltammetry (CV) and chronocoulometry (CC). CC is used to monitor the DNA hybridization event by measurement of electrostatic binding [Ru(NH3)6]3+. Under the optimal conditions, the signal of [Ru(NH3)6]3+ was linear with the logarithm of the concentration of the complementary oligonucleotides from 1.0 x 10(-13) to 1.0 x 10(-11) M, and the detection limit is 3.5 x 10(-14) M.
Wang
Jie
J
College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu 241000, People's Republic of China.
Zhang
Shaojun
S
Zhang
Yuzhong
Y
D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D017975:Ruthenium Compounds / Q000737:chemistry
D015342:DNA Probes; D017975:Ruthenium Compounds; C031584:hexammineruthenium; D011107:Polylysine; D002244:Carbon; D006046:Gold; D004247:DNA