title: In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines.
abstract: Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARgamma2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARgamma2-overexpressing BEFS cells (BEFS-PPARgamma2) grown in ADCM with or without the PPARgamma2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARgamma2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARgamma2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Yin
authors.forename: Xun
authors.initials: X
authors.lastname: Jin
authors.forename: Samuel
authors.initials: S
authors.lastname: Beck
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Kang
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Hong
authors.forename: Zhehu
authors.initials: Z
authors.lastname: Li
authors.forename: Yongcheng
authors.initials: Y
authors.lastname: Jin
authors.forename: Qiankun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
authors.forename: Sung-Chan
authors.initials: SC
authors.lastname: Kim
authors.forename: Hyunggee
authors.initials: H
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D047495:PPAR gamma