Regulation of AHI1 expression in adult rat brain: Implication in hypothalamic feeding control.
Recent studies revealed that Abelson helper integration site 1 (AHI1) plays a role in brain development. However, little is known about the role of AHI1 in adult brain. To directly assess the role of AHI1 in the adult brain, we cloned full-length cDNA of rat AHI1 and observed prominent expression of AHI1 in the hypothalamus, which contributes mainly to the control of energy homeostasis. Furthermore, we demonstrated that food deprivation caused induction of AHI1 in the hypothalamus and subsequent re-feeding down-regulated AHI1 expression, suggesting the involvement of AHI1 in feeding control. Moreover, the expression of AHI1 was increased in serum-depleted Neuro2A cells and restored by subsequent insulin treatment. Furthermore, treatment in food-deprived rat with intraperitoneal glucose also reduced the increased AHI1 expression. These results demonstrate that AHI1 expression can be regulated through diet and suggest the novel role of AHI1 in feeding behavior.
Han
Seung Baek
SB
Department of Anatomy, Division of Brain Korea 21 Biomedical Science, Korea University College of Medicine, Sungbuk-Gu, Seoul 136-705, Republic of Korea.
Choi
Byung-il
BI
Lee
Dongmin
D
Kee
Sun-Ho
SH
Kim
Hyeon Soo
HS
Sun
Woong
W
Kim
Hyun
H
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D004435:Eating / Q000502:physiology*; D005215:Fasting / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology; D008297:Male; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
C545896:Ahi1 protein, rat; D001786:Blood Glucose; D007328:Insulin; D011506:Proteins