title: Single-nucleotide variations associated with Mycobacterium tuberculosis KwaZulu-Natal strains.
abstract: The occurrence of drug resistance in Mycobacterium tuberculosis, the aetiological agent of tuberculosis (TB), is hampering the management and control of TB in the world. Here we present a computational analysis of recently sequenced drug-sensitive (DS), multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of M. tuberculosis. Single-nucleotide variations (SNVs) were identified in a pair-wise manner using the anchor-based whole genome comparison (ABWGC) tool and its modified version. For this analysis, four fully sequenced genomes of different strains of M. tuberculosis were taken along with three KwaZulu-Natal (KZN) strains isolated from South Africa including one XDR and one MDR strain. KZN strains were compared with other fully sequenced strains and also among each other. The variations were analysed with respect to their biological influence as a result of either altered structure or synthesis. The results suggest that the DR phenotype may be due to changes in a number of genes. The database on KZN strains can be accessed through the website http://mirna.jnu.ac.in/mgdd/.
authors.affiliation: Center for Computational Biology and Bioinformatics, School of Information Technology, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Sarbashis
authors.initials: S
authors.lastname: Das
authors.forename: Ragothaman M
authors.initials: RM
authors.lastname: Yennamalli
authors.forename: Anchal
authors.initials: A
authors.lastname: Vishnoi
authors.forename: Parul
authors.initials: P
authors.lastname: Gupta
authors.forename: Alok
authors.initials: A
authors.lastname: Bhattacharya
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D014644:Genetic Variation; D016680:Genome, Bacterial; D017720:Molecular Epidemiology / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D009711:Nucleotides / Q000235:genetics*; D013019:South Africa / Q000453:epidemiology; D014376:Tuberculosis / Q000235:genetics
chemical_list: D009711:Nucleotides