Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii.
Superoxide dismutases (SODs) play important role in stress tolerance of plants. In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana x P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC) and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants when exposed to NaCl stress. In addition, the relative weight gains of the transgenic plants were 8- to 23-fold of those observed for WT plants after NaCl stress for 30 days. The data showed that the SOD activities that increased in transgenic lines are 1.3-4-folds of that increased in the WT plant when exposed to NaCl stress. Our analysis showed that increases in SOD activities as low as 0.15-fold can also significantly enhance salt tolerance in transgenic plants, suggesting an important role of increased SOD activity in plant salt tolerance
Wang
Yu Cheng
YC
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, 26 Hexing Road, 150040 Harbin, China.
Qu
Guan Zheng
GZ
Li
Hong Yan
HY
Wu
Ying Jie
YJ
Wang
Chao
C
Liu
Gui Feng
GF
Yang
Chuan Ping
CP
D004553:Electric Conductivity; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008315:Malondialdehyde / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D032107:Populus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D055049:Salt Tolerance / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D032363:Tamaricaceae / Q000201:enzymology / Q000235:genetics*; D015854:Up-Regulation
D008315:Malondialdehyde; D013482:Superoxide Dismutase