title: Unravelling Escherichia coli dynamics close to the maximum growth temperature through heterogeneous modelling.
abstract: OBJECTIVE
  Previous work showed that the exponential phase of Escherichia coli K12 MG1655, grown in Brain Heart Infusion broth at temperatures close to its maximum growth temperature, is disturbed. Based on plate count data, microscopic images and literature, the existence of a heat-resistant subpopulation was hypothesized. Here, this hypothesis is mathematically explored via a heterogeneous model.
  
  
  RESULTS
  A heat-sensitive and a heat-resistant subpopulation are considered. A large fraction of the population is inactivated, while the remaining smaller fraction is able to resist (or adapt to) the inimical temperature and grows. A heterogeneous model that encloses a growth model (resistant population) and an inactivation model (sensitive population) is used to describe the global population dynamics. Most experimental data can be predicted when taking parameter uncertainty via Monte Carlo simulation into account.
  
  
  CONCLUSIONS
  The heterogeneous model accurately describes disturbed growth curves at superoptimal temperatures, except for high initial cell densities.
  
  
  CONCLUSIONS
  This study strengthens the hypothesis of the existence of a (small) heat-resistant subpopulation in typical inoculum cultures of E. coli K12 MG1655.
authors.affiliation: Department of Chemical Engineering, Katholieke Universiteit Leuven, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Van Derlinden
authors.forename: K
authors.initials: K
authors.lastname: Bernaerts
authors.forename: J F
authors.initials: JF
authors.lastname: Van Impe
mesh_terms: D015169:Colony Count, Microbial; D048168:Escherichia coli K12 / Q000254:growth & development* / Q000502:physiology; D006358:Hot Temperature; D008954:Models, Biological