Immobilized-OPG-Fc on a titanium surface inhibits RANKL-dependent osteoclast differentiation in vitro.
The purpose of the present study was to examine the effect of osteoprotegerin (OPG)-Fc fusion protein immobilized on a titanium surface on the initial differentiation of osteoclast precursor RAW264.7 cells. These cells were cultured on titanium specimens over which OPG-Fc was immobilized. The enhancement of tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expression in RAW264.7 cells exposed to receptor activator of NF-kappaB ligand (RANKL) stimulation on OPG-Fc-coated titanium was significantly lower than that in RAW264.7 cells exposed to RANKL on titanium specimens without immobilized OPG-Fc (ANOVA, P < 0.01). Preincubation of OPG-Fc-coated titanium, in a medium supplemented with 10% fetal bovine serum at 37 degrees C for two days before the cells were seeded, had no significant effect on the decrease in mRNA expression (ANOVA, P < 0.01). Taken together, these results indicate that OPG-Fc immobilized on a titanium surface blocks the differentiation of RAW264.7 cells induced by RANKL stimulation.
Makihira
Seicho
S
Department of Oral Biology and Engineering, Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan. seicho@hiroshima-u.ac.jp
Mine
Yuichi
Y
Nikawa
Hiroki
H
Shuto
Takahiro
T
Kosaka
Eduardo
E
Sugiyama
Masaru
M
Hosokawa
Ryuji
R
D000327:Adsorption; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008422:Materials Testing; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D053244:Osteoprotegerin / Q000737:chemistry* / Q000494:pharmacology*; D053245:RANK Ligand / Q000378:metabolism*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D007141:Immunoglobulin Fc Fragments; D053244:Osteoprotegerin; D053245:RANK Ligand; C506031:Tnfrsf11b protein, mouse; C505689:Tnfsf11 protein, mouse; D014025:Titanium