Cell adhesion in amphibian gastrulation.
The amphibian gastrula can be regarded as a single coherent tissue which folds and distorts itself in a reproducible pattern to establish the embryonic germ layers. It is held together by cadherins which provide the flexible adhesion required for the massive cell rearrangements that accompany gastrulation. Cadherin expression and adhesiveness increase as one goes from the vegetal cell mass through the anterior mesendoderm to the chordamesoderm, and then decrease again slightly in the ectoderm. Together with a basic random component of cell motility, this flexible, differentially expressed adhesiveness generates surface and interfacial tension effects which, in principle, can exert strong forces. However, conclusive evidence for an in vivo role of differential adhesion-related effects in gastrula morphogenesis is still lacking. The most important morphogenetic process in the amphibian gastrula seems to be intercellular migration, where cells crawl actively across each other's surface. The crucial aspect of this process is that cell motility is globally oriented, leading for example to mediolateral intercalation of bipolar cells during convergent extension of the chordamesoderm or to the directional migration of unipolar cells during translocation of the anterior mesendoderm on the ectodermal blastocoel roof. During these movements, the boundary between ectoderm and mesoderm is maintained by a tissue separation process.
Winklbauer
Rudolf
R
Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 3G5, Canada.
D000663:Amphibians / Q000378:metabolism*; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D054262:Gastrulation