Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates.
Cryptococcus neoformans was grown in 96-well microtiter plates sealed by foil which is less than 0.01 % permeable to oxygen. On day 14 of the cultivation, we observed peculiar clusters of small droplike daughter cells arranged around < or = 4 % of mother cells. The fact that most of the other cells had died indicates that few cells had been able to survive hypoxic conditions and escape the cell-cycle arrest. However, their daughters were unable to separate from them and to continue their proliferation under such conditions.
Raclavský
V
V
Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, 775 15 Olomouc, Czech Republic. rac@tunw.upol.cz
Pavlícek
J
J
Novotný
R
R
Moránová
Z
Z
Ohkusu
M
M
Trtková
J
J
Takeo
K
K
Kawamoto
S
S
D002455:Cell Division; D003455:Cryptococcus neoformans / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D010100:Oxygen / Q000378:metabolism*
D010100:Oxygen