title: Marine Pseudomonas putida: a potential source of antimicrobial substances against antibiotic-resistant bacteria.
abstract: Bacteria isolated from marine sponges found off the coast of Rio de Janeiro, Brazil, were screened for the production of antimicrobial substances. We report a new Pseudomonas putida strain (designated P. putida Mm3) isolated from the sponge Mycale microsigmatosa that produces a powerful antimicrobial substance active against multidrug-resistant bacteria. P. putida Mm3 was identified on the basis of 16S rRNA gene sequencing and phenotypic tests. Molecular typing for Mm3 was performed by RAPD-PCR and comparison of the results to other Pseudomonas strains. Our results contribute to the search for new antimicrobial agents, an important strategy for developing alternative therapies to treat infections caused by multidrug-resistant bacteria.
authors.affiliation: Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Palloma Rodrigues
authors.initials: PR
authors.lastname: Marinho
authors.forename: Ana Paula Barbosa
authors.initials: AP
authors.lastname: Moreira
authors.forename: Flávia Lúcia Piffano Costa
authors.initials: FL
authors.lastname: Pellegrino
authors.forename: Guilherme
authors.initials: G
authors.lastname: Muricy
authors.forename: Maria do Carmo de Freire
authors.initials: Mdo C
authors.lastname: Bastos
authors.forename: Kátia Regina Netto dos
authors.initials: KR
authors.lastname: Santos
authors.forename: Marcia
authors.initials: M
authors.lastname: Giambiagi-deMarval
authors.forename: Marinella Silva
authors.initials: MS
authors.lastname: Laport
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009792:Oceans and Seas; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D016958:Pseudomonas putida / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S