title: Angiogenic and angiostatic chemokines in idiopathic pulmonary fibrosis and granulomatous lung disease.
abstract: BACKGROUND
  Angiogenesis-angiostasis balance and leukocyte recruitment are influenced by different concentrations of distinct chemokines.
  
  
  OBJECTIVE
  To investigate the relative contribution of angiogenic and angiostatic CXC chemokines to the pathogenesis of idiopathic pulmonary fibrosis (IPF) and granulomatous lung diseases, we examined the in vitro production of an angiogenic chemokine (IL-8), and 2 angiostatic chemokines (IP-10 and MIG) by alveolar macrophages.
  
  
  METHODS
  Alveolar macrophages from 16 patients with granulomatous lung diseases [8 with sarcoidosis, 8 with extrinsic allergic alveolitis (EAA)], 16 patients with IPF, and 8 control subjects were cultured for 24 h. IL-8, IL-18, IP-10 and MIG in the culture supernatants were measured by a fluorescent bead-based multiplex technique.
  
  
  RESULTS
  In IPF patients, IL-8 was increased and correlated with bronchoalveolar lavage (BAL) neutrophils, whereas the levels of IP-10 and MIG were normal. In sarcoidosis and EAA patients, IL-8, IP-10, and MIG were all increased and IP-10 and MIG correlated with IL-18, a Th1 cytokine, and the percentage and number of BAL lymphocytes.
  
  
  CONCLUSIONS
  The difference in the expression of CXC chemokines and a Th1 cytokine may contribute to the different immunopathogenesis, clinical course and responsiveness to treatment of these diseases.
authors.affiliation: Pneumology and Allergology, Ruhrlandklinik, Medical Faculty, University of Duisburg-Essen, Tueschener Weg 40, Essen, Germany.
authors.forename: Ai
authors.initials: A
authors.lastname: Cui
authors.forename: Olaf
authors.initials: O
authors.lastname: Anhenn
authors.forename: Dirk
authors.initials: D
authors.lastname: Theegarten
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Ohshimo
authors.forename: Francesco
authors.initials: F
authors.lastname: Bonella
authors.forename: Stephan U
authors.initials: SU
authors.lastname: Sixt
authors.forename: Juergen
authors.initials: J
authors.lastname: Peters
authors.forename: Rafael
authors.initials: R
authors.lastname: Sarria
authors.forename: Josune
authors.initials: J
authors.lastname: Guzman
authors.forename: Ulrich
authors.initials: U
authors.lastname: Costabel
mesh_terms: D000368:Aged; D000542:Alveolitis, Extrinsic Allergic / Q000276:immunology* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016022:Case-Control Studies; D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000378:metabolism; D054370:Chemokine CXCL9 / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D006801:Humans; D020382:Interleukin-18 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D011658:Pulmonary Fibrosis / Q000276:immunology* / Q000378:metabolism; D017565:Sarcoidosis, Pulmonary / Q000276:immunology* / Q000378:metabolism
chemical_list: D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; D020382:Interleukin-18; D016209:Interleukin-8