title: [Development of new SSR markers from EST of SSH cDNA libraries on rose fragrance].
abstract: The new SSR markers of rose related fragrance were developed based on the SSH cDNA libraries of rose floral scent mutant. In this study, 10 EST-SSRs (2.6%) from 391 ESTs in the libraries were identified. Six EST-SSRs primers were designed to sequence flanking SSRs. The primer pairs designed were screened on the wild-type Jinyindao, which has flowers full of pleasant scent, and the mutant-type Wangriqinghuai without perceivable floral scent. Five primer pairs were amplified effectively in Jinyindao and Wangriqinghuai, and 3 were polymorphic between Jinyindao and Wangriqinghuai. Eighteen rose cultivars including fragrant roses and nonfragrant roses were identified by the five prime pairs. These results proved that EST-SSR markers are effective markers to identify the polymorphism of the rose.
authors.affiliation: Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China. hjyan8203@126.com
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Yan
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Ji-Rong
authors.initials: JR
authors.lastname: Xie
authors.forename: Shu-Fa
authors.initials: SF
authors.lastname: Li
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Jian
authors.forename: Xian-Qin
authors.initials: XQ
authors.lastname: Qiu
authors.forename: Qi-Gang
authors.initials: QG
authors.lastname: Wang
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Kai-Xue
authors.initials: KX
authors.lastname: Tang
mesh_terms: D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D009812:Odorants; D027826:Rosa / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D005819:Genetic Markers