[Association of expression of progesterone and estrogen-alpha receptors with the activity of argyrophilic proteins in nucleolar organizer regions in smooth muscle tumors of the corpus uteri].
The immunohistochemical technique was used to study the levels progesterone and estrogen-alpha receptors in the smooth muscle cells, by examining the specimens taken from 168 leiomyosarcomas (LMS) and 106 leiomyomas (LM) of the corpus uteri. The presence and degree of receptor expression were shown to be related to the proliferative activity (PA) (Ki67 protein expression), the activity of argyrophilic proteins in the nucleolar organizer region (Ag-NOR-protein) (silver-plating with AgNO3, as described by Y. Daskal) and the proteins regulating the apoptotic system (Bcl-2 expression) (93.4 +/- 1.1%). The presence of sex hormone receptors in LMS was associated with low PA (8.5 +/- 3.3%), the decreased synthesis of Ag-NOR proteins (15.1 +/- 2.7%), and high Bcl-2 activity (93.4 +/- 1.1%) although the presence of the receptors had no significant effect on p53. The negative expression of both types of receptors was characteristic for LMS and associated with high PA (19.3 +/- 3.1%), the high expression of Ag-NOR-proteins (25.3 +/- 2.6%), and a drastic reduction in the amplification of Bcl-2 (38.4 +/- 10%) in reference to the positive receptor status. Receptor coexpression was associated with relatively low PA, the expression of argyrophilic proteins and the relatively high expression Bcl-2 without any noticeable impact on the expression of p53.
Avdalian
A M
AM
Bobrov
I P
IP
Klimachev
V V
VV
Chechulin
M N
MN
Lazarev
A F
AF
D014408:Biomarkers, Tumor / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D007890:Leiomyosarcoma / Q000378:metabolism* / Q000473:pathology; D009697:Nucleolus Organizer Region / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology*
D014408:Biomarkers, Tumor; D047628:Estrogen Receptor alpha; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D011980:Receptors, Progesterone; D016159:Tumor Suppressor Protein p53