Effects of siRNAs targeting the spacer sequence of plant RNAi vectors on the specificity and efficiency of RNAi.
Small interfering RNAs (siRNAs) generated from the spacer region of hairpin RNAs were not detected in the RNA interference (RNAi) plants targeting the fatty acid desaturase gene. The expression of the desaturase gene was stably suppressed even when siRNAs targeting the spacer sequences were introduced into this plant.
Nishimura
Kazuma
K
Graduate School of Horticulture, Chiba University, 648 Matsudo, Chiba 271-8510, Japan. a_pony8217@yahoo.co.jp
Hirai
Sayaka
S
Kodama
Hiroaki
H
D044943:Fatty Acid Desaturases / Q000235:genetics*; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D017962:alpha-Linolenic Acid / Q000378:metabolism*
D034741:RNA, Small Interfering; D017962:alpha-Linolenic Acid; D044943:Fatty Acid Desaturases