title: Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart.
abstract: Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have tested published approaches and challenged their practicality. As a result, we propose an improved and streamlined protocol, which consistently yields high-quality histology, thereby minimizing experimental noise and group sizes.
authors.affiliation: Department of Cardiovascular Medicine, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom. sbohl@well.ox.ac.uk
authors.forename: Steffen
authors.initials: S
authors.lastname: Bohl
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Medway
authors.forename: Jeanette
authors.initials: J
authors.lastname: Schulz-Menger
authors.forename: Jurgen E
authors.initials: JE
authors.lastname: Schneider
authors.forename: Stefan
authors.initials: S
authors.lastname: Neubauer
authors.forename: Craig A
authors.initials: CA
authors.lastname: Lygate
mesh_terms: D000818:Animals; D004396:Coloring Agents; D004195:Disease Models, Animal; D007090:Image Interpretation, Computer-Assisted; D007211:Indoles; D051379:Mice; D009203:Myocardial Infarction / Q000473:pathology*; D015428:Myocardial Reperfusion Injury / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D009942:Organometallic Compounds; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents; D007211:Indoles; D009942:Organometallic Compounds; C015445:copper phthalocyanine