title: Water-assisted CO(2) laser ablated glass and modified thermal bonding for capillary-driven bio-fluidic application.
abstract: The glass-based microfluidic chip has widely been applied to the lab-on-a-chip for clotting tests. Here, we have demonstrated a capillary driven flow chip using the water-assisted CO(2) laser ablation for crackless fluidic channels and holes as well as the modified low-temperature glass bonding with assistance of adhesive polymer film at 300 degrees Celsius. Effect of water depth on the laser ablation of glass quality was investigated. The surface hydrophilic property of glass and polymer film was measured by static contact angle method for hydrophilicity examination in comparison with the conventional polydimethylsiloxane (PDMS) material. Both low-viscosity deionized water and high-viscosity whole blood were used for testing the capillary-driving flow behavior. The preliminary coagulation testing in the Y-channel chip was also performed using whole blood and CaCl(2) solution. The water-assisted CO(2) laser processing can cool down glass during ablation for less temperature gradient to eliminate the crack. The modified glass bonding can simplify the conventional complex fabrication procedure of glass chips, such as high-temperature bonding, long consuming time and high cost. Moreover, the developed fluidic glass chip has the merit of hydrophilic behavior conquering the problem of traditional hydrophobic recovery of polymer fluidic chips and shows the ability to drive high-viscosity bio-fluids.
authors.affiliation: Department of Mechanical Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, Taiwan. ckchung@mail.ncku.edu.tw
authors.forename: C K
authors.initials: CK
authors.lastname: Chung
authors.forename: H C
authors.initials: HC
authors.lastname: Chang
authors.forename: T R
authors.initials: TR
authors.lastname: Shih
authors.forename: S L
authors.initials: SL
authors.lastname: Lin
authors.forename: E J
authors.initials: EJ
authors.lastname: Hsiao
authors.forename: Y S
authors.initials: YS
authors.lastname: Chen
authors.forename: E C
authors.initials: EC
authors.lastname: Chang
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.forename: C C
authors.initials: CC
authors.lastname: Lin
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D001709:Biotechnology / Q000295:instrumentation*; D002197:Capillary Action; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005898:Glass / Q000737:chemistry* / Q000528:radiation effects; D006358:Hot Temperature; D054020:Lasers, Gas; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
chemical_list: D001672:Biocompatible Materials