title: Development and characterization of new microsatellite markers for the oyster mushroom (Pleurotus ostreatus).
abstract: We developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (Pleurotus ostreatus). In total, 169 alleles were identified with an average of 4.7 alleles per locus. Values for observed (HO) and expected (HE) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. Nineteen loci deviated from Hardy-Weinberg equilibrium. Significant (P<0.05) excess heterozygosity was observed at nine loci. Linkage disequilibrium (LD) was significant (P<0.05) between pairs of locus alleles. Cluster analysis revealed that five species of genus Pleurotus made a distinct group, and the individual cultivars were grouped into major five groups from G-1 to G-5. The diverse cultivars of P. ostreatus were discriminated and the other four species revealed a different section in the UPGMA tree. These microsatellite markers proved to be very useful tools for genetic studies, including assessment of the diversity and population structure of P. ostreatus.
authors.affiliation: National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea.
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Ma
authors.forename: Gi-An
authors.initials: GA
authors.lastname: Lee
authors.forename: Sok-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Jae-Gyun
authors.initials: JG
authors.lastname: Gwag
authors.forename: Tae-San
authors.initials: TS
authors.lastname: Kim
authors.forename: Won-Sik
authors.initials: WS
authors.lastname: Kong
authors.forename: Kyoung-In
authors.initials: KI
authors.lastname: Seo
authors.forename: Gang-Seob
authors.initials: GS
authors.lastname: Lee
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Park
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D017931:DNA Primers; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics; D006580:Genetic Carrier Screening; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D020076:Pleurotus / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D018744:DNA, Plant