title: Long term effects on human plasma lipoproteins of a formulation enriched in butter milk polar lipid.
abstract: BACKGROUND
  Sphingolipids (SL), in particular sphingomyelin (SM) are important components of milk fat polar lipids. Dietary SM inhibits cholesterol absorption in rats (Nyberg et al. J Nutr Biochem. 2000) and SLs decrease both cholesterol and TG concentrations in lipid- and cholesterol fed APOE*3Leiden mice (Duivenvoorden et al. Am J Clin Nutr. 2006). This human study examines effects of a butter milk formulation enriched in milk fat globule membrane material, and thereby in SLs, on blood lipids in healthy volunteers. In a four week parallel group study with 33 men and 15 women we examined the effects of an SL-enriched butter milk formulation (A) and an equivalent control formulation (B) on plasma lipid levels. Plasma concentrations of HDL and LDL cholesterol, triacylglycerols (TG), apolipoproteins AI and B, and lipoprotein (a) were measured. The daily dose of SL in A was 975 mg of which 700 mg was SM. The participants registered food and drink intake four days before introducing the test formula and the last four days of the test period.
  
  
  RESULTS
  A daily increase of SL intake did not significantly influence fasting plasma lipids or lipoproteins. In group B TG, cholesterol, LDL, HDL and apolipoprotein B concentrations increased, however, but not in group A after four weeks. The difference in LDL cholesterol was seen primarily in women and difference in TG primarily in men. No significant side effects were observed.
  
  
  CONCLUSIONS
  The study did not show any significant decrease on plasma lipids or lipoprotein levels of an SL-enriched formulation containing 2-3 times more SL than the normal dietary intake on cholesterol, other plasma lipids or on energy intake. The formulation A may, however, have counteracted the trend towards increased blood lipid concentrations caused by increased energy intake that was seen with the B formulation.
authors.affiliation: Department of Clinical Sciences, Medicine (Gastroenterology and Nutrition), Lund University Hospital, S221 85 Lund, Sweden. Lena.Ohlsson@med.lu.se
authors.forename: Lena
authors.initials: L
authors.lastname: Ohlsson
authors.forename: Hans
authors.initials: H
authors.lastname: Burling
authors.forename: Ake
authors.initials: A
authors.lastname: Nilsson
mesh_terms: D000328:Adult; D000818:Animals; D002079:Butter / Q000032:analysis*; D002417:Cattle; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000737:chemistry*
chemical_list: D004041:Dietary Fats; D008074:Lipoproteins; D002079:Butter