title: The E. coli single protein production system for production and structural analysis of membrane proteins.
abstract: At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.
authors.affiliation: Center for Advanced Technology and Medicine, UMDNJ-Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA.
authors.forename: Lili
authors.initials: L
authors.lastname: Mao
authors.forename: S Thangminlal
authors.initials: ST
authors.lastname: Vaiphei
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Shimazu
authors.forename: William M
authors.initials: WM
authors.lastname: Schneider
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Tang
authors.forename: Rajeswari
authors.initials: R
authors.lastname: Mani
authors.forename: Monica J
authors.initials: MJ
authors.lastname: Roth
authors.forename: Gaetano T
authors.initials: GT
authors.lastname: Montelione
authors.forename: Masayori
authors.initials: M
authors.lastname: Inouye
mesh_terms: D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D011506:Proteins