Arsenite tolerance and biotransformation potential in estuarine bacteria.
Bacterial isolates from water and sediment samples from freshwater, estuarine and marine regions were tested for their growth in the presence of different concentrations of arsenic. Despite the generation times being longer in case of all bacterial isolates tested in nutrient broth with 200 ppm Arsenite (As(3+)), many of them were able to attain log phase and substantial growth variously between 36 and 96 h. The isolates tolerating >or=200 ppm arsenic (As) were found to belong to Enterobacteriaceae, Pseudomonas, Corynebaterium, Xanthomonas, Acinetobacter, Flavimonas and Micrococcus. Some of these environmental strains tolerant to 1,000 ppm arsenic were tested to realize their potential to detoxify arsenic. The rate of As biotransformation was faster by many of these strains. The percent of arsenite biotransformed/removed from the growth medium was the highest by a strain of Enterobacteriaceae (as much as 92% of the As in the growth medium by 120 h) followed by that of Corynebaterium and Acinetobacter strains. From these observations it is clear that many environmental strains are capable of quite rapid biotransformation of As. Contamination of drinking water by toxic metalloid arsenic affects thousands of people worldwide. Many environmental isolates of bacteria which detoxify this metalloid would serve beneficial in the depuration processes. We suggest that only such strains capable of high tolerance to toxic arsenite, would biotransform As in polluted estuarine environments and would prove useful in As bioremediation applications.
Nagvenkar
Geeta S
GS
National Institute of Oceanography, Council of Scientific and Industrial Research (CSIR), Dona Paula, Goa, 403004, India.
Ramaiah
N
N
D018053:Arsenites / Q000378:metabolism / Q000633:toxicity*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001673:Biodegradation, Environmental; D001711:Biotransformation; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance; D005618:Fresh Water / Q000382:microbiology; D019015:Geologic Sediments / Q000382:microbiology; D007194:India; D007700:Kinetics; D012623:Seawater / Q000382:microbiology; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D018508:Water Purification / Q000379:methods
D018053:Arsenites; D014874:Water Pollutants, Chemical; C015001:arsenite