RNA interference effectively degrades mRNA and inhibits protein expression of GBV-C E2 gene in Huh7 cells.
The GB virus C/hepatitis G virus (GBV-C/HGV) is a Flaviviridae member that despite its nonpathogenicity, has become of great interest given that it could inhibit the replication of the human immunodeficiency virus (HIV). Therefore, a better knowledge of the viral protein E2 has become our aim. In this study, a GBV-C model cell system (HuhEG) which expressing a fusion protein of the GBV-C E2 protein and enhanced green fluorescent protein (EGFP) stably was established. And the expression of these proteins was silenced effectively by the two E2 gene-specific siRNAs and an EGFP gene-specific siRNA. This inhibition is sequence-specific and extensive (90%). This HuhEG/specific siRNAs system can provide an approach for investigating the association between GBV-C E2 and HIV replication, which may be of potential value in the development of novel prophylactic or therapeutic agents for HIV infection.
Cao
Ming-Mei
MM
Department of Microbiology, Second Military Medical University, Shanghai, China.
Li
Gang
G
Ren
Hao
H
Pan
Wei
W
Zhao
Ping
P
Qi
Zhong-Tian
ZT
D002460:Cell Line; D029407:GB virus C / Q000276:immunology*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D006801:Humans; D034622:RNA Interference; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000037:antagonists & inhibitors; D014764:Viral Proteins / Q000037:antagonists & inhibitors*
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins