[Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization].
OBJECTIVE
To study the relationship between the inhibitory effects of Tongxie Yaofang, a compound traditional Chinese herbal medicine, on the contraction of the colonic smooth muscle isolated from rats and calcium mobilization.


METHODS
By measuring the tension of the isolated colonic smooth muscle strips, the inhibitory effects of Tongxie Yaofang on the contraction induced by acetylcholine (ACh), KCl and exhausting Ca(2+) of internal calcium store were assessed respectively.


RESULTS
Tongxie Yaofang could concentration-dependently inhibit the contraction of isolated rat colonic smooth muscle strips induced by KCl and exhausting the Ca(2+) of internal calcium store. Tongxie Yaofang could also inhibit the tension of the second contractile phase induced by ACh (P<0.01, vs control), but had no influence on the first contractile phase.


CONCLUSIONS
Tongxie Yaofang can inhibit the contraction of isolated rat colonic smooth muscle strips mainly by preventing the influx of extracellular Ca(2+), which may be associated with blocking voltage-dependent channel, store-operated channel and receptor-operated channel, but not by preventing the release of internal Ca(2+) from calcium store.
Yuan
Jian-ye
JY
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Xie
Jian-qun
JQ
Wu
Da-zheng
DZ
Zheng
Yu
Y
Pan
Xiang-xue
XX
Fei
Xiao-yan
XY
Xu
Hai-zhen
HZ
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D002118:Calcium