Evaluation of antidiabetic activity of polysaccharide isolated from Phellinus linteus in non-obese diabetic mouse.
Polysaccharide (PLP) isolated from Phellinus linteus inhibits tumor growth and metastasis by enhancing immune functions of macrophages, dendritic cells, NK cells, T cells, and B cells. Here, we report that PLP can inhibit the development of autoimmune diabetes in non-obese diabetic (NOD) mice. Although 80% of the NOD mice had developed diabetes by 24 weeks of age, none of the PLP-treated NOD mice developed diabetes. The mean blood glucose levels were 110mg/dl in PLP-treated mice and 499mg/dl in control NOD mice. Histological examination of the pancreatic islets revealed that most of the islets isolated from PLP-treated mice were less infiltrated with lymphocytes compared with those of control mice. Spleen cells from diabetic NOD mice could adaptively transfer diabetes into NOD/SCID mice, but those from PLP-treated NOD mice showed delayed transfer of diabetes. PLP inhibited the expression of inflammatory cytokines, including IFN-gamma, IL-2, and TNF-alpha by Th1 cells and macrophages, but up-regulated IL-4 expression by Th2 cells in NOD mice. PLP did not prevent streptozotocin-induced diabetic development in ICR mice. Taken together, these results suggest that PLP inhibits the development of autoimmune diabetes by regulating cytokine expression.
Kim
Hwan Mook
HM
Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Republic of Korea.
Kang
Jong Soon
JS
Kim
Jee Youn
JY
Park
Song-Kyu
SK
Kim
Hyung Sook
HS
Lee
Young June
YJ
Yun
Jieun
J
Hong
Jin Tae
JT
Kim
Youngsoo
Y
Han
Sang-Bae
SB
D019264:Adoptive Transfer; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000276:immunology; D001835:Body Weight / Q000187:drug effects; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D018450:Disease Progression; D005260:Female; D005658:Fungi; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D016688:Mice, Inbred NOD; D010179:Pancreas / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000084682:Phellinus; D010936:Plant Extracts; D011134:Polysaccharides / Q000008:administration & dosage* / Q000009:adverse effects; D013311:Streptozocin / Q000008:administration & dosage; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology / Q000637:transplantation; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000637:transplantation
D001786:Blood Glucose; D016207:Cytokines; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D013311:Streptozocin