title: Dynamin inhibitor impairs Toxoplasma gondii invasion.
abstract: The protozoan parasite Toxoplasma gondii infects its host cells through an active mechanism. In this work, we obtained evidence that host cells also play a fundamental role during the infection process. We found that previous incubation of the host cells, but not the parasites, with Dynasore, a small molecule that inhibits dynamin GTPase activity, markedly reduced the penetration of T. gondii tachyzoites into LLC-MK2 cells. In contrast, parasite adhesion to the host cell surface increased, as observed both by light and electron microscopy. Intriguingly, the few parasites internalized by Dynasore-treated cells remained in vacuoles located at the periphery of the cell, in contrast to the perinuclear localization seen in the control.
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Lucio Ayres
authors.initials: LA
authors.lastname: Caldas
authors.forename: Márcia
authors.initials: M
authors.lastname: Attias
authors.forename: Wanderley
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D034281:Dynamins / Q000037:antagonists & inhibitors*; D006790:Host-Parasite Interactions; D006801:Humans; D006835:Hydrazones / Q000008:administration & dosage; D008253:Macaca mulatta; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D014122:Toxoplasma / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D014123:Toxoplasmosis / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D014617:Vacuoles / Q000378:metabolism / Q000469:parasitology
chemical_list: D006835:Hydrazones; C511794:N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide; D034281:Dynamins