Durable mesenchymal stem cell labelling by using polyhedral superparamagnetic iron oxide nanoparticles.
Small polyhedral superparamagnetic iron oxide (SPIO) nanoparticles (<10 nm) coated with a thin layer of silica were prepared (SPIO@SiO(2) and SPIO@SiO(2)-NH(2)). Surface modification of the small polyhedral silica-coated SPIO nanoparticles with amines led to substantially higher mesenchymal stem cell (MSC) labelling efficiency without the use of additional transfecting agents. Therefore, amine surface-modified nanoparticles (SPIO@ SiO(2)-NH(2)) appeared to be the preferred candidate for MSC labelling. In vitro studies demonstrated that controlled labelling of SPIO@SiO(2) and SPIO@SiO(2)-NH(2) did not cause MSC death or proliferation inhibition. MSCs labelled with SPIO@SiO(2)-NH(2) nanoparticles retained differentiation potential and showed osteogenic, adipogenic and chondrogenic differentiations. The noncytotoxic polyhedral SPIO@SiO(2)-NH(2) nanoparticle-labelled MSCs were successfully implanted in rabbit brain and erector spinae muscle, and demonstrated long-lasting, durable MRI labelling efficacy after 8-12 weeks.
Wang
Hao-Hao
HH
Department of Diagnostic Radiology and Organ Imaging, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
Wang
Yi-Xiang J
YX
Leung
Ken Cham-Fai
KC
Au
Doris W T
DW
Xuan
Shouhu
S
Chak
Chun-Pong
CP
Lee
Simon K M
SK
Sheng
Hui
H
Zhang
Ge
G
Qin
Ling
L
Griffith
James F
JF
Ahuja
Anil T
AT
D000588:Amines / Q000737:chemistry*; D000818:Animals; D003287:Contrast Media / Q000737:chemistry* / Q000493:pharmacokinetics; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D007501:Iron / Q000493:pharmacokinetics*; D008279:Magnetic Resonance Imaging / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053758:Nanoparticles; D011817:Rabbits; D011990:Receptors, Transferrin / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry*
D000588:Amines; D003287:Contrast Media; D011990:Receptors, Transferrin; D012822:Silicon Dioxide; D007501:Iron; D052203:Ferrosoferric Oxide