title: In vitro comparison of three caries dyes.
abstract: OBJECTIVE
  To evaluate the efficacy of a conventional and newly-developed caries detector (Caries Check) by measuring the Vickers microhardness (VMH) and DIAGNOdent value of the dentin cavity wall of extracted human teeth after removing carious dentin that was stained by the caries detector.
  
  
  METHODS
  30 human teeth with coronal dentin caries extending halfway through the dentin were sectioned and extracted for use in this study. As a control, the longitudinal section of a caries-free extracted human tooth was prepared. The VMH and DIAGNOdent values were measured and the microstructure of the dentin cavity wall was examined using a scanning electron microscope (SEM).
  
  
  RESULTS
  When Caries Check was employed as a guide for carious dentin removal, the microhardness of the cavity wall was significantly lower than the normal dentin and the DIAGNOdent value indicated that the dentin may have recalcified. SEM observations of the stained regions revealed that Caries Check did not stain the sclerotic dentin. Based on these findings, Caries Check may be a useful modality because the excavation of the dentin structure was shallower and the sclerotic dentin was preserved.
authors.affiliation: Department of Clinical Cariology, Showa University, School of Dentistry, 2-1-1 Kitasenzoku, Ohta-Ward, Tokyo 145-8515, Japan. kitoh@dent.showa-u.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Itoh
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kusunoki
authors.forename: Misa
authors.initials: M
authors.lastname: Oikawa
authors.forename: Chihiiro
authors.initials: C
authors.lastname: Tani
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hisamitsu
mesh_terms: D001557:Benzenesulfonates; D004396:Coloring Agents; D003731:Dental Caries / Q000473:pathology* / Q000628:therapy; D003737:Dental Cavity Preparation / Q000295:instrumentation / Q000379:methods*; D003804:Dentin / Q000648:ultrastructure; D003809:Dentin, Secondary / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006244:Hardness; D006801:Humans; D007834:Lasers; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D014677:Pharmaceutical Vehicles; D011108:Polymers; D019946:Propylene Glycol; D011409:Propylene Glycols; D012235:Rhodamines; D012394:Rosaniline Dyes
chemical_list: D001557:Benzenesulfonates; D004396:Coloring Agents; D005456:Fluorescent Dyes; D014677:Pharmaceutical Vehicles; D011108:Polymers; D011409:Propylene Glycols; D012235:Rhodamines; D012394:Rosaniline Dyes; C012504:polypropylene glycol; C022027:lissamine rhodamine B; C025485:basic fuchsin; D019946:Propylene Glycol; C006796:brilliant blue