Protective effect of curcumin against kainic acid induced seizures and oxidative stress in rats.
The effect of curcumin, a dietary antioxidant was studied against kainic acid (KA)-induced seizures and on markers of oxidative stress. Rats were administered KA (10 mg/kg, ip) and observed for behavioral changes, incidence and latency of convulsions and mortality over four hours. The rats were thereafter sacrificed for estimation of oxidative stress parameters; malondialdehyde (MDA) and glutathione (GSH). Curcumin was administered 30 min before KA at doses of 50, 100 and 200 mg/kg, ip. KA induced long-lasting seizures and associated symptoms. The brain level of MDA was significantly (P < 0.05) raised after KA administration (536 +/- 44 nmol/g wet tissue) as compared to saline treated group (200 +/- 36 nmol/g wet tissue) and significantly decreased the levels of GSH. Pretreatment with curcumin (100 and 200 mg/kg, ip) significantly increased the latency of seizures (120 + 20 min and 11 5+/- 5.7 min respectively) as compared to the vehicle treated KA group. Curcumin (100 and 200 mg/ kg, ip) significantly prevented the increase in MDA levels and ameliorated the fall in glutathione. Curcumin at the dose of 50 mg/kg had no effect on any of oxidative stress parameters. The study reports the potential antiepileptic effect of antioxidant curcumin.
Gupta
Yogendra K
YK
Neuropharmacology Laboratory, Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110 029. yk.ykgupta@gmail.com
Briyal
Seema
S
Sharma
Monisha
M
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D007608:Kainic Acid; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control*
D008315:Malondialdehyde; D005978:Glutathione; D003474:Curcumin; D007608:Kainic Acid