title: Decreased blastocyst production in mice exposed to increased rack noise.
abstract: This study was conducted to investigate the possible effect of rack type on the blastocyst yield of mouse embryo donors. The first phase of the study consisted of housing some mice (group A) in a ventilated rack and others (group B) in a static rack in the same room for 3 d, followed by euthanasia for blastocyst collection and corticosterone assay. Parametric tests were used to compare groups. The number of blastocysts per donor was lower in group A (5.0 +/- 1.4 blastocysts) than group B (13.1 +/- 3.7 blastocysts). Mean noise was higher in the ventilated rack (80.4 dBC) than in the static rack (69.2 dBC). Serum corticosterone concentrations did not differ between groups. For the second phase of the study, a third group of mice (group C) was housed in a static rack without a ventilated rack in the same room. The noise level for group C was even lower (45.18 +/- 2.91 dBC), and the blastocyst count per donor (16.4 +/- 2.4) was higher than that of group B. The mean noise levels of empty ventilated and static racks differed significantly between groups for 10 different sound frequencies. Plotting mean blastocyst production against mean rack noise revealed a negative linear relationship with good strength of correlation. These results support the earlier observation that decreased blastocyst count occurs following housing of bred C57BL/6 donor mice in ventilated cages.
authors.affiliation: David Geffen School of Medicine, University of California, Los Angeles, CA, USA. bzamora@ufl.edu
authors.forename: Bernadette M
authors.initials: BM
authors.lastname: Zamora
authors.forename: Meisheng
authors.initials: M
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Minghua
authors.initials: M
authors.lastname: Chai
authors.forename: P Timothy
authors.initials: PT
authors.lastname: Lawson
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Lawson
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D003345:Corticosterone / Q000097:blood; D005260:Female; D006799:Housing, Animal; D007756:Laboratory Animal Science; D051379:Mice / Q000196:embryology*; D008810:Mice, Inbred C57BL; D009622:Noise; D014691:Ventilation
chemical_list: D003345:Corticosterone