[New putative control elements in the promoter OF CXCL13 chemokine gene, a target of alternative NF-kappaB pathway].
We searched the proximal promoter region of CXCL13/BLC chemokine gene for new putative control elements, including potential NF-kappaB binding sites. Using electrophoretic mobility shift assay and reporter gene analysis we identified two new promoter elements. The first element contains Rel/NF-kappaB binding site and seems to participate in inducible gene expression, while another site binds transcription factor Sp1 and is critical for basic transcription. It is the first indication that alternative NF-kappaB pathway target genes are probably cooperatively controlled by factors Rel/NF-kappaB and Sp1. Identification of a functional Sp1 site in the promoter of a target gene of alternative NF-kappaB pathway will be useful for investigation of molecular mechanisms and signal pathwaysinvolved.
Britanova
L V
LV
Kuprash
D V
DV
D000818:Animals; D002460:Cell Line; D054382:Chemokine CXCL13 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism
C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; C486386:Cxcl13 protein, mouse; D016328:NF-kappa B; D016329:Sp1 Transcription Factor