title: Development and evaluation of a multiplex polymerase chain reaction assay to identify Salmonella serogroups and serotypes.
abstract: To improve limitations of Salmonella serotyping, 2 multiplex polymerase chain reaction (M-PCR) were developed using a strategy that identifies first the genes encoding serogroups (rfbJ, wzx). According to the serogroup determined, a second M-PCR identifies serotype (fliC, fljB, wcdB, and sdf-I sequence). Standardization and evaluation of both M-PCRs were carried out.
authors.affiliation: Laboratorio de Microbiología, Instituto Colombiano de Medicina Tropical - Universidad CES Cra 43 A #52 Sur 99, Medellín, Colombia. ncardona@ces.edu.co
authors.forename: Nora
authors.initials: N
authors.lastname: Cardona-Castro
authors.forename: Miryan
authors.initials: M
authors.lastname: Sánchez-Jiménez
authors.forename: Lelia
authors.initials: L
authors.lastname: Lavalett
authors.forename: Nélida
authors.initials: N
authors.lastname: Múñoz
authors.forename: Jaime
authors.initials: J
authors.lastname: Moreno
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012475:Salmonella / Q000145:classification* / Q000235:genetics; D012703:Serotyping / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers