Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation.
Matrix metalloproteinase-9 (MMP-9) is important in the pathogenesis of periodontitis. Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) is involved in MMP-9 up-regulation in human monocytes. We tested the hypothesis that cPLA(2)alpha also regulates MMP-9 induction by Fusobacterium nucleatum and by phorbol 12-myristate-13-acetate (PMA) in gingival epithelial cells. While PMA induced MMP-9 expression considerably, F. nucleatum did so moderately. This time-course study demonstrated that MMP-9 mRNA up-regulation occurred at 3 hours, whereas MMP-9 secretion and activity in cell-free supernatants occurred at 12 hours. cPLA(2)alpha mRNA was constitutively expressed in gingival epithelial cells. Transient activation of cPLA(2) by Ser505 phosphorylation was observed in the nuclei upon stimulation, suggesting its role as a transcription factor, while cPLA(2) protein expression remained unchanged. Induction of MMP-9 expression and activity was significantly inhibited by 1 muM of the specific cPLA(2)alpha inhibitor (P < 0.01). These findings demonstrate the involvement of cPLA(2)alpha in MMP-9 up-regulation.
Montreekachon
P
P
Center of Excellence for Innovation in Chemistry, Department of Odontology and Oral Pathology, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand.
Chotjumlong
P
P
Reutrakul
V
V
Krisanaprakornkit
S
S
D002273:Carcinogens / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D004789:Enzyme Activation; D004790:Enzyme Induction / Q000502:physiology; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000201:enzymology*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology; D054513:Group IV Phospholipases A2 / Q000037:antagonists & inhibitors / Q000502:physiology*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D010766:Phosphorylation; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014157:Transcription Factors / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D002273:Carcinogens; D014157:Transcription Factors; D048051:p38 Mitogen-Activated Protein Kinases; D054513:Group IV Phospholipases A2; C516389:PLA2G4A protein, human; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate