Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification.
Loading and releasing protein in a controllable way is extremely important for the protein vehicles used in bone tissue engineering. To obtain a suitable carrier material for basic proteins, such as BMP or bFGF, hydroxyapatite particles containing mercaptosuccinic acid (mercaptosuccinic acid (Mer), (Mer-HAp)) were synthesized. Physicochemical evaluation of Mer-HAp suggested that Mer was contained in HAp particles: it either simply adsorbed onto HAp crystals or was trapped among the HAp crystals. A protein adsorption study using basic and acidic model proteins indicated that the synthesized Mer-HAp had selective loading properties of the basic protein. The loaded protein was gradually released from Mer-HAp in phosphate buffered saline. The protein release rate was different in each Mer-HAp synthesized with a different concentration of Mer. In addition, the protein release from Mer-HAp showed a similar profile with the Ca dissolution in different pH solutions, indicating that the Mer-HAp dissolution was concerned with the protein release from Mer-HAp. Thus, Mer-HAp is a useful candidate for the basic protein carrier because it has properties which enable the loading and releasing of protein in a controllable way.
Ishihara
Shiho
S
Department of Materials Science, Osaka Prefecture University, Japan.
Matsumoto
Takuya
T
Onoki
Takamasa
T
Uddin
Mohammad Hafiz
MH
Sohmura
Taiji
T
Nakahira
Atsushi
A
D018786:Bone Substitutes / Q000737:chemistry*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry*; D013870:Thiomalates / Q000737:chemistry*
D018786:Bone Substitutes; D004337:Drug Carriers; D011506:Proteins; D013870:Thiomalates; D017886:Durapatite; C046062:2-thiomalic acid