title: The water-deficit stress- and red-rot-related genes in sugarcane.
abstract: Sugarcane is an important international commodity as a valuable agricultural crop especially in developing countries. Sequencing was carried out to generate >35,000 expressed sequence tags (ESTs) from healthy as well as red-rot-infected tissue of Indian subtropical variety of sugarcane. Subsequent clustering with existing sugarcane ESTs in public databases identified 4,087 clusters, including 85 clusters that preferentially express upon Colletotrichum falcatum (red-rot) infection, which were previously unreported. Real-time reverse transcription-PCR profiling of selected EST clusters identified several sugarcane clusters that show differential expression in response to biotic and abiotic stress conditions. Twenty-five stress-related clusters showed >2-fold relative expression during water-deficit stress in sugarcane. Similarly, EST clusters could be identified, which exhibit association with red-rot disease when assessed in red-rot-susceptible and red-rot-resistant varieties of sugarcane. Such EST clusters are good candidates for in-depth analysis to elucidate stress-responsive pathways in sugarcane and facilitate genetic manipulation to tailor this crop for tolerance to various stresses.
authors.affiliation: Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110 021, India.
authors.forename: Vikrant
authors.initials: V
authors.lastname: Gupta
authors.forename: Saurabh
authors.initials: S
authors.lastname: Raghuvanshi
authors.forename: Ambika
authors.initials: A
authors.lastname: Gupta
authors.forename: Navin
authors.initials: N
authors.lastname: Saini
authors.forename: Anupama
authors.initials: A
authors.lastname: Gaur
authors.forename: M S
authors.initials: MS
authors.lastname: Khan
authors.forename: R S
authors.initials: RS
authors.lastname: Gupta
authors.forename: J
authors.initials: J
authors.lastname: Singh
authors.forename: S K
authors.initials: SK
authors.lastname: Duttamajumder
authors.forename: S
authors.initials: S
authors.lastname: Srivastava
authors.forename: A
authors.initials: A
authors.lastname: Suman
authors.forename: Jitendra P
authors.initials: JP
authors.lastname: Khurana
authors.forename: Raman
authors.initials: R
authors.lastname: Kapur
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Tyagi
mesh_terms: D001483:Base Sequence; D016000:Cluster Analysis; D020231:Colletotrichum / Q000502:physiology*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D007700:Kinetics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031786:Saccharum / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics*; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water