Evaluation of an animal product-free variant of MegaCell MEM as a storage medium for corneas destined for transplantation.
OBJECTIVE
The traditional medium for storing corneas in European Eye Banks is Gibco's MEM (Eagle's) with Earle's salts and Hepes containing 2% fetal calf serum, glutamine and antimycotics. Although serum-free MegaCell MEM has been reported to be more suitable for this purpose, it contains components of animal origin that potentially pose health risks to corneal recipients. The possibility of removing or replacing these components has therefore been investigated.


METHODS
A MegaCell basal medium (DME) and a formulation of this (MegaCell DCS), which contains no components of animal origin, have been prepared. The viability of the endothelial, epithelial and stromal cells of corneas held in these media has been assessed, their stress levels monitored and water content determined.


RESULTS
The endothelial cell count and morphology of corneas held in MegaCell DME and DCS for 30 days remained little changed. Their epithelial and stromal cells also retained their ability to proliferate in culture. Neither DME nor DCS prevented corneal swelling but the lack of endogenous MMP-2 activation indicated that the corneas were not subject to metabolic stress.


CONCLUSIONS
MegaCell DCS is an animal product-free medium formulated for corneal storage. The quality of corneas held in this medium is similar to those held in MegaCell MEM.
Smith
Valerie A
VA
Academic Unit of Ophthalmology, University of Bristol, Bristol Eye Hospital, Bristol, UK. Val.Smith@bristol.ac.uk
Johnson
Terrell
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D003319:Corneal Stroma / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D016039:Corneal Transplantation / Q000379:methods*; D007562:Creutzfeldt-Jakob Syndrome / Q000209:etiology; D016895:Culture Media, Serum-Free / Q000737:chemistry / Q000494:pharmacology*; D004728:Endothelium, Corneal / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005125:Eye Banks / Q000379:methods; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
D016895:Culture Media, Serum-Free; D019188:Organ Preservation Solutions; D053505:Matrix Metalloproteinases, Secreted