title: Real-time imaging of histone H4 hyperacetylation in living cells.
abstract: To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer (FRET)-based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetylation changes in response to structurally distinct histone deacetylase inhibitors.
authors.affiliation: Chemical Genetics Laboratory/Chemical Genomics Research Group, RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Sasaki
authors.forename: Tamaki
authors.initials: T
authors.lastname: Ito
authors.forename: Norikazu
authors.initials: N
authors.lastname: Nishino
authors.forename: Saadi
authors.initials: S
authors.lastname: Khochbin
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004791:Enzyme Inhibitors / Q000494:pharmacology; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D015151:Immunoblotting; D008239:Lysine / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008938:Mitosis; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection
chemical_list: C485182:Cyan Fluorescent Protein; D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C012589:trichostatin A; D006655:Histone Deacetylases; D008239:Lysine