Ultrastructural implications of pretreatment for successful cryopreservation of Oncidium protocorm-like body.
By applying pre-treatment with high concentrations of sucrose and glycerol prior to desiccation and subsequent freezing in liquid nitrogen, successful cryopreservation with high recovery rate was achieved in Oncidium PLB (protocorm-like body). Cellular and subcellular changes after each step in various cryopreservation regimes were examined to elucidate the mechanism of the beneficial effect of the pretreatment which confers resistance to desiccation and freezing.
Miao
Nae-Hwey
NH
Department of Regulation-Biology, Faculty of Science, Saitama University, Saitama, Japan.
Kaneko
Yasuko
Y
Sugawara
Yasutake
Y
D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003890:Desiccation; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D029595:Orchidaceae / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure*; D013395:Sucrose / Q000494:pharmacology
D003451:Cryoprotective Agents; D013395:Sucrose; D005990:Glycerol