title: Unusual 22S-spirostane steroids from Dracaena cambodiana.
abstract: From the methanol extract of Dracaena cambodiana roots two unusual 22S-spirostane steroids (1beta,3beta,14alpha,20R,22S,25R)-spirost-5-ene-1,3,14-triol (1) and (lbeta,3beta,14alpha,15alpha,20R,22S,25R)-spirost-5-ene-tetrol (2) have been isolated, together with a known 22R-spirostane compound, namogenin B (3). Their structures were elucidated by spectroscopic and spectrometric methods, including HRMS and extensive 1D and 2D NMR spectroscopy. Compound 1 showed significant antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus and Fusarium oxysporum, with MIC values of 45.0, 25.0 and 50.0 microg/mL, respectively.
authors.affiliation: Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Nguyen Tien
authors.initials: NT
authors.lastname: Dat
authors.forename: Nguyen Hai
authors.initials: NH
authors.lastname: Dang
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nam
authors.forename: Ninh Khac
authors.initials: NK
authors.lastname: Ban
authors.forename: Nguyen Van
authors.initials: NV
authors.lastname: Tuyen
authors.forename: Le Mai
authors.initials: le M
authors.lastname: Huong
authors.forename: Tran Thu
authors.initials: TT
authors.lastname: Huong
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D031409:Dracaena / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010936:Plant Extracts; D013141:Spiro Compounds; D013256:Steroids