Efficient transposition of Tol2 in the mouse germline.
Insertional mutagenesis screens play an integral part in the annotating of functional data for all sequenced genes in the postgenomic era. Chemical mutagenesis screens are highly efficient but identifying the causative gene can be a laborious task. Other mutagenesis platforms, such as transposable elements, have been successfully applied for insertional mutagenesis screens in both the mouse and rat. However, relatively low transposition efficiency has hampered their use as a high-throughput forward genetic mutagenesis screen. Here we report the first evidence of germline activity in the mouse using a naturally active DNA transposon derived from the medaka fish called Tol2, as an alternative system for high-throughput forward genetic mutagenesis screening tool.
Keng
Vincent W
VW
Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
Ryan
Barbara J
BJ
Wangensteen
Kirk J
KJ
Balciunas
Darius
D
Schmedt
Christian
C
Ekker
Stephen C
SC
Largaespada
David A
DA
D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D005854:Germ Cells / Q000378:metabolism*; D006367:HeLa Cells; D057166:High-Throughput Screening Assays; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation
D004251:DNA Transposable Elements