Isolation and functional characterization of DgZFP: a gene encoding a Cys2/His2-type zinc finger protein in chrysanthemum.
A Cys2/His2-type zinc finger protein gene, DgZFP, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach. The DgZFP encodes a protein of 211 amino acids residues with a calculated molecular mass of 22.9 kDa and theoretical isoelectric point is 8.59. DgZFP contains two Cys2/His2-type zinc finger motifs, one nuclear localization domain, one Leu-rich domain, and one ethylene-responsive element-binding factor (ERF)-associated amphiphilic repression (EAR) domain. The transcript of DgZFP was enriched in flowers than in roots, stems, and leaves of the adult chrysanthemum plants. The gene expression was strongly induced by NaCl, drought and cold treatment, and weakly by ABA treatment in the seedlings. Subcellular localization revealed that DgZFP was localized preferentially distributed to nucleus. Overexpression of DgZFP improved salt tolerance and resulted in growth suppression in transgenic tobacco. We argued that DgZFP is a new member of the Cys2/His2-type zinc finger protein genes, and it maybe function as a regulator in response to salt stress in plants.
Liu
Qing-Lin
QL
Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China. qinglinliu@126.com
Xu
Ke-Dong
KD
Ma
Nan
N
Zeng
Li
L
Zhao
Liang-Jun
LJ
D002467:Cell Nucleus / Q000378:metabolism; D031202:Chrysanthemum / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003545:Cysteine / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006639:Histidine / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000254:growth & development; D055049:Salt Tolerance / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D014018:Tissue Distribution; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics
D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D006639:Histidine; D003545:Cysteine