Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Kynurenine aminotransferases (KATs) catalyze the synthesis of kynurenic acid (KYNA), an endogenous antagonist of N-methyl-D: -aspartate and alpha 7-nicotinic acetylcholine receptors. Abnormal KYNA levels in human brains are implicated in the pathophysiology of schizophrenia, Alzheimer's disease, and other neurological disorders. Four KATs have been reported in mammalian brains, KAT I/glutamine transaminase K/cysteine conjugate beta-lyase 1, KAT II/aminoadipate aminotransferase, KAT III/cysteine conjugate beta-lyase 2, and KAT IV/glutamic-oxaloacetic transaminase 2/mitochondrial aspartate aminotransferase. KAT II has a striking tertiary structure in N-terminal part and forms a new subgroup in fold type I aminotransferases, which has been classified as subgroup Iepsilon. Knowledge regarding KATs is vast and complex; therefore, this review is focused on recent important progress of their gene characterization, physiological and biochemical function, and structural properties. The biochemical differences of four KATs, specific enzyme activity assays, and the structural insights into the mechanism of catalysis and inhibition of these enzymes are discussed.
Han
Qian
Q
Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
Cai
Tao
T
Tagle
Danilo A
DA
Li
Jianyong
J
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology*; D020134:Catalytic Domain; D006801:Humans; D007736:Kynurenic Acid / Q000378:metabolism; D051379:Mice; D017434:Protein Structure, Tertiary; D000637:Transaminases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
D000637:Transaminases; C021481:kynurenine-oxoglutarate transaminase; D007736:Kynurenic Acid