title: C-type natriuretic peptide (CNP) is a bifurcation factor for sensory neurons.
abstract: Neuronal circuits are shaped during development by the coordinated action of guidance factors and signals that regulate axonal branching. Unlike guidance cues, the molecules and signaling cascades that underlie axonal branching remain to be resolved. Here we show that the secreted molecule C-type natriuretic peptide (CNP) induces a cGMP signaling cascade via its receptor particulate guanylyl cyclase Npr2 which is essential for sensory axon bifurcation at the dorsal root entry zone (DREZ) of the spinal cord. In contrast, another form of sensory axon branching-collateral formation-is not affected by this pathway. We also demonstrate that cGMP signaling via the nitric oxide-stimulated soluble guanylyl cyclase system (NO-GC) is dispensable for sensory axon branching. Functionally, the bifurcation error in CNP mutant mice is maintained at mature stages and results in a reduced input on secondary neurons as detected by patch-clamp recordings.
authors.affiliation: Department of Developmental Neurobiology, Max Delbrück Center for Molecular Medicine, Robert Rössle Strasse 10, Berlin, Germany.
authors.forename: Hannes
authors.initials: H
authors.lastname: Schmidt
authors.forename: Agne
authors.initials: A
authors.lastname: Stonkute
authors.forename: René
authors.initials: R
authors.lastname: Jüttner
authors.forename: Doris
authors.initials: D
authors.lastname: Koesling
authors.forename: Andreas
authors.initials: A
authors.lastname: Friebe
authors.forename: Fritz G
authors.initials: FG
authors.lastname: Rathjen
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D020098:Natriuretic Peptide, C-Type / Q000235:genetics / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism*; D015398:Signal Transduction; D013116:Spinal Cord / Q000378:metabolism; D013126:Spinal Nerve Roots / Q000378:metabolism; D000099242:Atrial Natriuretic Factor Receptor B
chemical_list: D006152:Cyclic GMP; D020098:Natriuretic Peptide, C-Type; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B