Characterization of Bombyx mori parvo-like virus non-structural protein NS1.
NS1 gene of Bombyx mori parvo-like virus (China Zhenjiang isolate, BmDNV-Z) codes a predicted 316-amino acid protein, but its function remains unknown. Results of the current study showed that purified recombinant 6 x His-NS1 protein possesses ATP binding, ATPase, DNA binding, and helicase activities. Only one protein was captured in infected Bombyx mori midgut cells against NS1 target protein by employing co-immunoprecipitation, which was identified to be a viral protein by mass spectrometry. The NS1-interacting protein is encoded by BmDNV-Z ORF4 and its molecular is about 100 kD. Analysis of His pull-down confirmed that binding of identified viral protein to purified recombinant 6 x His-NS1 protein in vitro. Taken together, our results indicated that BmDNV-Z NS1 was a multifunctional protein, which may be involved with virus replication.
Li
Guohui
G
Institute of Life Sciences, Jiangsu University, Zhenjiang, China.
Sun
Chen
C
Zhang
Junhong
J
He
Yuanqing
Y
Chen
Huiqing
H
Kong
Jie
J
Huang
Guoping
G
Chen
Keping
K
Yao
Qin
Q
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000821:virology*; D002681:China; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000235:genetics / Q000302:isolation & purification*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D000251:Adenosine Triphosphatases