Protective efficacy of bacterial membranes containing surface-exposed BM95 antigenic peptides for the control of cattle tick infestations.
The Rhipicephalus (Boophilus) microplus BM86 and BM95 glycoproteins are homologous proteins that protect cattle against tick infestations. In this study, we demonstrated that the recombinant chimeric protein comprising tick BM95 immunogenic peptides fused to the A. marginale MSP1a N-terminal region for presentation on the Escherichia coli membrane was protective against R. microplus infestations in rabbits. This system provides a novel and simple approach for the production of tick protective antigens by surface display of antigenic protein chimera on live E. coli and suggests the possibility of using recombinant bacterial membrane fractions for vaccination against cattle tick infestations.
Canales
Mario
M
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005 Ciudad Real, Spain.
Labruna
Marcelo B
MB
Soares
João F
JF
Prudencio
Carlos R
CR
de la Fuente
José
J
D000709:Anaplasma / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D004926:Escherichia coli / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D048494:Rhipicephalus; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014612:Vaccines / Q000276:immunology*
C085065:BM86 protein, Boophilus; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014612:Vaccines