Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells.
Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients. Furthermore, iPS cells can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells. hASCs can be safely and readily isolated from adult humans in large quantities without extended time for expansion, are easy to maintain in culture, and therefore represent an ideal autologous source of cells for generating individual-specific iPS cells.
Sun
Ning
N
Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Panetta
Nicholas J
NJ
Gupta
Deepak M
DM
Wilson
Kitchener D
KD
Lee
Andrew
A
Jia
Fangjun
F
Hu
Shijun
S
Cherry
Athena M
AM
Robbins
Robert C
RC
Longaker
Michael T
MT
Wu
Joseph C
JC
D017667:Adipocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D054337:Cell Dedifferentiation / Q000235:genetics / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism
D015703:Antigens, CD; D000954:Antigens, Surface; D011509:Proteoglycans; C107064:TRA-1-60 antigen, human; D000469:Alkaline Phosphatase