Simulation of stroke-related damage in cultured human nerve cells.
We describe a novel cell culture protocol for the generation of neurons from a human teratocarcinoma cell line. These neurons were used to investigate hypoxic-ischaemic cell damage and for developing neuroprotective strategies. Cultures of human model neurons should eventually serve to reduce the number of experimental animals in cerebral stroke research.
Bicker
Gerd
G
University of Veterinary Medicine, Division Cell Biology, Hannover, Germany. gerd.bicker@tiho-hannover.de
Gierse
Andrea
A
Tan
Saime
S
Paquet-Durand
François
F
D000826:Animal Testing Alternatives; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D020521:Stroke / Q000473:pathology*; D018243:Teratocarcinoma / Q000473:pathology; D013736:Testicular Neoplasms / Q000473:pathology
D018696:Neuroprotective Agents