Influence of genetic background on fluoride metabolism in mice.
A/J and 129P3/J mouse strains have different susceptibilities to dental fluorosis, due to their genetic backgrounds. This study tested whether these differences are due to variations in water intake and/or F metabolism. A/J (susceptible to dental fluorosis) and 129P3/J mice (resistant) received drinking water containing 0, 10, or 50 ppm F. Weekly F intake, excretion and retention, and terminal plasma and femur F levels were determined. Dental fluorosis was evaluated clinically and by quantitative fluorescence (QF). Data were tested by two-way ANOVA. Although F intakes by the strains were similar, excretion by A/J mice was significantly higher due to greater urinary F excretion, which resulted in lower plasma and femur F levels. Compared with 129P3/J mice given 50 ppm F, significantly higher QF scores were recorded for A/J mice. In conclusion, these strains differ with respect to several features of F metabolism, and amelogenesis in the 129P3/J strain seems to be unaffected by high F exposure.
Carvalho
J G
JG
Department of Biological Sciences, Bauru Dental School, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru-SP 17012-901, São Paulo, Brazil. mbuzalaf@fob.usp.br
Leite
A L
AL
Yan
D
D
Everett
E T
ET
Whitford
G M
GM
Buzalaf
M A R
MA
D000042:Absorption; D000566:Amelogenesis / Q000187:drug effects; D000818:Animals; D001835:Body Weight; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D004326:Drinking; D004435:Eating; D005243:Feces / Q000737:chemistry; D005269:Femur / Q000737:chemistry; D005453:Fluorescence; D005459:Fluorides / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D009050:Fluorosis, Dental / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D007180:Incisor / Q000473:pathology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008815:Mice, Inbred Strains
D002327:Cariostatic Agents; D005459:Fluorides