title: Dietary supplementation with alpha-amylase inhibitor wheat albumin to high-fat diet-induced insulin-resistant rats is associated with increased expression of genes related to fatty acid synthesis in adipose tissue.
abstract: It is well-known that insulin resistance induces lipid abnormalities by decreasing insulin actions in adipose tissue. This study examined the effects of inhibiting postprandial hyperglycemia/hyperinsulinemia, using the alpha-amylase inhibitor wheat albumin (WA), on the expression of genes related to fatty acid metabolism in the adipose tissue of high-fat diet-induced insulin-resistant rats. Postprandial glucose and insulin levels were significantly lower after oral starch loading with WA than with inactivated WA in insulin-resistant rats. In addition, the increases in the plasma triacylglycerol and insulin levels by feeding insulin-resistant rats a control diet were inhibited by WA supplementation. Supplementation with WA increased the mRNA levels of not only fatty acid synthase (FAS) and acyl-CoA carboxylase (ACC) but also their transcriptional factors such as carbohydrate response element-binding protein (ChREBP) and sterol regulatory element binding protein (SREBP)1 in the mesenteric adipose tissue of the insulin-resistant rats. In addition, supplementation with WA tended to increase the protein expression levels of FAS and ACCs. These results suggest that reductions in the plasma triacylglycerol and insulin levels by inhibiting hyperglycemia/hyperinsulinemia with the alpha-amylase inhibitor WA in high-fat diet-induced insulin-resistant rats are associated with increased expression of genes related to fatty acid synthesis and their transcriptional factors in adipose tissue.
authors.affiliation: The University of Shizuoka Graduate School of Nutritional and Environmental Sciences and Global COE, Shizuoka, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Murayama
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Masaya
authors.initials: M
authors.lastname: Shimada
authors.forename: Saki
authors.initials: S
authors.lastname: Fujimoto
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nukui
authors.forename: Kenji
authors.initials: K
authors.lastname: Shibata
authors.forename: Toshinao
authors.initials: T
authors.lastname: Goda
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D019587:Dietary Supplements; D005227:Fatty Acids / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D007333:Insulin Resistance; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D055314:Seed Storage Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D012333:RNA, Messenger; D055314:Seed Storage Proteins; D000516:alpha-Amylases