Co-production of gamma-glutamylcysteine and glutathione by mutant strain Saccharomyces cerevisiae FC-3 and its kinetic analysis.
Co-production of gamma -glutamylcysteine (gamma -GC) and glutathione (GSH) by a novel mutant strain Saccharomyces cerevisiae FC-3 and its kinetic analysis were investigated. The strain could produce gamma -GC and GSH with high yields (4.22 and 14.3 mg/g-DCW, respectively) in batch submerged cultures. Effects of medium components and cultivation conditions on cell growth and the contents of intracellular gamma -GC and GSH were examined. Results show that 2% (w/v) sucrose and 2.5% (w/v) yeast extract are the best carbon and nitrogen sources, respectively, and supplement of three amino acids (glycine, cysteine and glutamate), each at 0.08% (w/v), in the medium could enhance gamma -GC and GSH production. In addition, optimal operating conditions are at the initial pH value of 7.0, 30 degrees C and 200 rev/min. Moreover, results obtained from kinetic analyses reveal that gamma -GC production is mixed-type growth associated, but GSH production is growth-associated.
Chen
When-Chang
WC
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan, ROC. chenwc@yuntech.edu.tw
Huang
Fu-Kuo
FK
Cheng
Shu-Chuan
SC
Tsai
Feng-Yuan
FY
Lin
Chung-Liang
CL
D000596:Amino Acids / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism*; D004151:Dipeptides / Q000096:biosynthesis*; D005285:Fermentation; D005978:Glutathione / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D013696:Temperature
D000596:Amino Acids; D003470:Culture Media; D004151:Dipeptides; D013395:Sucrose; D002244:Carbon; D005978:Glutathione; C017341:gamma-glutamylcysteine; D009584:Nitrogen