[Effect of osteopontin silencing by lentivirus-mediated delivery of siRNA on glioma cell invasion and apoptosis].
OBJECTIVE
To investigate the effect of osteopontin silencing on the invasion and apoptosis of U251 cells.


METHODS
The invasion, apoptosis and levels of uPA, MMP-2 and MMP-9 were determined by invasion assay, flow cytometry, Western blot and real-time fluorescence quantitative PCR respectively.


RESULTS
Osteopontin small interference RNA (siRNA) inhibited osteopontin expression and cell invasion, promoted apoptosis in U251 cells. In addition, the expression of Bcl-2, uPA, MMP-2 and MMP-9 was decreased, while Bax level was elevated.


CONCLUSIONS
Osteopontin siRNA can inhibit U251 cells invasion via the down-regulation of uPA, MMP-2 and MMP-9 levels, and promote apoptosis through induction of Bax expression and inhibition of Bcl 2 level. It suggests that osteopontin plays an important role in human glioma progression.
Yan
Wei
W
Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, PR China.
Qian
Chun-fa
CF
Shi
Lei
L
Qian
Jin
J
Fu
Zhen
Z
Liu
Ning
N
Lu
Ai-lin
AL
You
Yong-ping
YP
D017209:Apoptosis; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*
D034741:RNA, Small Interfering; D053495:Osteopontin