Use of a saline gradient for the diagnosis of schistosomiasis.
The development of novel methods for parasitological diagnosis that are both highly sensitive and low in cost has been strongly recommended by the World Health Organization. In this study, a new technique for diagnosis of schistosomiasis mansoni is proposed based on the differential sedimentation of eggs when subjected to a slow continuous flux of 3% saline solution through a porous plaque. This influx suspends low-density faecal material, effectively cleaning the sample. The remaining sediment covering the porous plaque surface is then transferred to a glass slide and examined under a bright field microscope. Twelve Kato-Katz slides were used for comparison in the present study. Our results suggest that the saline gradient method detects a significantly higher number of eggs than the 12 Kato-Katz slides (p < 0.0001). We also found microscopic inspection to be quicker and easier with our newly described method. After cleaning the sample, the obtained sediment can also be conserved in a 10% formaldehyde solution and examined for at least 45 days later without statistically significant egg count differences.
Coelho
Paulo Marcos Z
PM
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou-Fiocruz, Av. Augusto de Lima 1715, Belo Horizonte, MG, Brazil. coelhopm@cpqrr.fiocruz.br
Jurberg
Arnon D
AD
Oliveira
Aureo A
AA
Katz
Naftale
N
D000818:Animals; D005243:Feces / Q000469:parasitology*; D006801:Humans; D010270:Parasite Egg Count / Q000295:instrumentation / Q000379:methods*; D012550:Schistosoma mansoni / Q000302:isolation & purification*; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D012965:Sodium Chloride
D012965:Sodium Chloride