Synthetic peptides containing a conserved sequence motif of the Id protein family modulate vascular smooth muscle cell phenotype.
Modulation of smooth muscle cells to a proliferating and migrating phenotype with downregulated alpha-actin expression is observed upon vascular lesion formation. The Id proteins (inhibitors of cell differentiation) play a role in the development of this phenotype. In contrast, synthetic peptides based on a conserved 11-residue Id sequence trigger the switch to a contractile phenotype that shows reduced cell growth and migration, increased expression of alpha-actin and decreased Id protein levels.
Pellegrino
Sara
S
DISMAB-Dipartimento di Scienze Molecolari Applicate ai Biosistemi-Sez. Chimica Organica, Università degli Studi di Milano, I-20133 Milan, Italy.
Ferri
Nicola
N
Colombo
Noemi
N
Cremona
Edoardo
E
Corsini
Alberto
A
Fanelli
Roberto
R
Gelmi
Maria Luisa
ML
Cabrele
Chiara
C
D000199:Actins / Q000502:physiology; D020816:Amino Acid Motifs / Q000502:physiology*; D001013:Aorta, Thoracic; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D017124:Conserved Sequence / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology; D009687:Nuclear Proteins; D010455:Peptides / Q000138:chemical synthesis / Q000502:physiology*; D010641:Phenotype; D011485:Protein Binding; D012097:Repressor Proteins
D000199:Actins; D002135:Calcium-Binding Proteins; D051796:Inhibitor of Differentiation Proteins; D009687:Nuclear Proteins; D010455:Peptides; D012097:Repressor Proteins