Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum.
In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle. The 70% decreased activity of isocitrate dehydrogenase, achieved by start codon exchange, resulted in a >40% improved lysine production. By flux analysis, this could be correlated to a flux shift from the TCA cycle toward anaplerotic carboxylation.
Becker
Judith
J
Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaussstr. 17, D-38106 Braunschweig, Germany.
Klopprogge
Corinna
C
Schröder
Hartwig
H
Wittmann
Christoph
C
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002952:Citric Acid Cycle; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005818:Genetic Engineering; D008239:Lysine / Q000096:biosynthesis* / Q000378:metabolism
D001426:Bacterial Proteins; D008239:Lysine