title: Rabies glycoprotein fused with B subunit of cholera toxin expressed in tobacco plants folds into biologically active pentameric protein.
abstract: The pentameric B subunit of cholera toxin (CtxB) is an efficient mucosal adjuvant for vaccines. We report the expression of a chimeric protein comprising the synthetic cholera toxin B subunit fused at its C-terminal with rabies surface glycoprotein (G protein) in tobacco plants. The approximately 80.3 kDa fusion polypeptide expressed at 0.4% of the total soluble protein in leaves of the selected transgenic lines. The fusion protein formed a approximately 403 kDa pentameric protein which was functionally active in binding to GM1 receptor. The plant-made protein had a higher affinity for GM1 receptor than the native bacterial CtxB. The pentameric fusion protein was recognized by the anti-cholera toxin as well as anti-rabies antibodies. Its immuno-protective ability against rabies remains to be examined.
authors.affiliation: National Botanical Research Institute, Council for Scientific and Industrial Research, Rana Pratap Marg, UP, Lucknow 226001, India.
authors.forename: Sribash
authors.initials: S
authors.lastname: Roy
authors.forename: Antariksh
authors.initials: A
authors.lastname: Tyagi
authors.forename: Siddharth
authors.initials: S
authors.lastname: Tiwari
authors.forename: Ankit
authors.initials: A
authors.lastname: Singh
authors.forename: Samir V
authors.initials: SV
authors.lastname: Sawant
authors.forename: Pradhyumna K
authors.initials: PK
authors.lastname: Singh
authors.forename: Rakesh
authors.initials: R
authors.lastname: Tuli
mesh_terms: D000956:Antigens, Viral / Q000235:genetics*; D002772:Cholera Toxin / Q000235:genetics*; D022121:Cholera Vaccines / Q000276:immunology; D006023:Glycoproteins / Q000235:genetics*; D017510:Protein Folding; D055503:Protein Multimerization; D011819:Rabies Vaccines / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D022121:Cholera Vaccines; D006023:Glycoproteins; D011819:Rabies Vaccines; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; D002772:Cholera Toxin