[Development of new SSR markers from EST of SSH cDNA libraries on rose fragrance].
The new SSR markers of rose related fragrance were developed based on the SSH cDNA libraries of rose floral scent mutant. In this study, 10 EST-SSRs (2.6%) from 391 ESTs in the libraries were identified. Six EST-SSRs primers were designed to sequence flanking SSRs. The primer pairs designed were screened on the wild-type Jinyindao, which has flowers full of pleasant scent, and the mutant-type Wangriqinghuai without perceivable floral scent. Five primer pairs were amplified effectively in Jinyindao and Wangriqinghuai, and 3 were polymorphic between Jinyindao and Wangriqinghuai. Eighteen rose cultivars including fragrant roses and nonfragrant roses were identified by the five prime pairs. These results proved that EST-SSR markers are effective markers to identify the polymorphism of the rose.
Yan
Hui-Jun
HJ
Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China. hjyan8203@126.com
Zhang
Hao
H
Xie
Ji-Rong
JR
Li
Shu-Fa
SF
Jian
Hong-Ying
HY
Qiu
Xian-Qin
XQ
Wang
Qi-Gang
QG
Wang
Ji-Hua
JH
Tang
Kai-Xue
KX
D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D009812:Odorants; D027826:Rosa / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*
D005819:Genetic Markers