title: [Screening and breeding of high lysine-producing strains by genome shuffling].
abstract: OBJECTIVE
  To screen and breed high lysine-producing strains by genome shuffling.
  
  
  METHODS
  Corynebacterium pekinense 1 was used as the starting strain in this study. High lysine-producing strains were screened by genome shuffling.
  
  
  RESULTS
  Five hereditarily stable strains with high lysine production were obtained by four cycles of genome shuffling. A high lysine producing strain, F4-36, was obtained, which produced 16.95 g/dL lysine, 37.1% higher than that of the starting strain.
  
  
  CONCLUSIONS
  Genome shuffling can be an efficient tool to screen and breed high lysine production strains.
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China. zk395@yahoo.com.cn
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.forename: Dongpo
authors.initials: D
authors.lastname: Zhou
mesh_terms: D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism*; D032621:DNA Shuffling; D016680:Genome, Bacterial; D008239:Lysine / Q000378:metabolism*
chemical_list: D008239:Lysine