Activation of defense response pathways by OGs and Flg22 elicitors in Arabidopsis seedlings.
We carried out transcriptional profiling analysis in 10-d-old Arabidopsis thaliana seedlings treated with oligogalacturonides (OGs), oligosaccharides derived from the plant cell wall, or the bacterial flagellin peptide Flg22, general elicitors of the basal defense response in plants. Although detected by different receptors, both OGs and Flg22 trigger a fast and transient response that is both similar and comprehensive, and characterized by activation of early stages of multiple defense signaling pathways, particularly JA-associated processes. However, the response to Flg22 is stronger in both the number of genes differentially expressed and the amplitude of change. The magnitude of induction of individual genes is in both cases dose-dependent, but, even at very high concentrations, OGs do not induce a response that is as comprehensive as that seen with Flg22. While high doses of either microbe-associated molecular pattern (MAMP) elicit a late response that includes activation of senescence processes, SA-dependent secretory pathway genes and PR1 expression are substantially induced only by Flg22. These results suggest a lower threshold for activation of early responses than for sustained or SA-mediated late defenses. Expression patterns of amino-cyclopropane-carboxylate synthase genes also implicate ethylene biosynthesis in regulation of the late innate immune response.
Denoux
Carine
C
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, 185 Cambridge St, Simches 7700, Boston, MA 02114, USA.
Galletti
Roberta
R
Mammarella
Nicole
N
Gopalan
Suresh
S
Werck
Danièle
D
De Lorenzo
Giulia
G
Ferrari
Simone
S
Ausubel
Frederick M
FM
Dewdney
Julia
J
D000375:Aging / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D005408:Flagellin / Q000494:pharmacology*; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000187:drug effects; D007700:Kinetics; D009844:Oligosaccharides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects*
D001426:Bacterial Proteins; D009844:Oligosaccharides; D017382:Reactive Oxygen Species; C412763:oligogalacturonic acid; D005408:Flagellin