In situ single cell monitoring by isocyanide-functionalized Ag and Au nanoprobe-based Raman spectroscopy.
The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanide functionalized silver and gold nanoparticles by surface enhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at approximately 2,100 cm-1 in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and 3,600 cm-1. The SERS marker band at approximately 2,100 cm-1 could be used to judge the location of the isocyanide-functionalized nanoparticles inside the cell without much spectral interference from other cellular constituents. Our results demonstrate that isocyanide-modified silver or gold nanoparticle-based SERS may have high potential for monitoring and imaging the biological processes at the single cell level.
Lee
So Yeong
SY
Department of Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Jang
Soo Hwa
SH
Cho
Myung-Haing
MH
Kim
Young Min
YM
Cho
Keun Chang
KC
Ryu
Pan Dong
PD
Gong
Myoung-Seon
MS
Joo
Sang-Woo
SW
D000818:Animals; D019556:COS Cells / Q000648:ultrastructure; D002477:Cells / Q000648:ultrastructure*; D002522:Chlorocebus aethiops; D003486:Cyanides; D006046:Gold; D007202:Indicators and Reagents; D017953:Isocyanates; D008322:Mammals / Q000502:physiology; D008854:Microscopy, Electron; D053758:Nanoparticles; D012542:Scattering, Radiation; D012834:Silver; D013059:Spectrum Analysis, Raman / Q000379:methods
C476736:2-methylpentane-diisocyanate; D003486:Cyanides; D007202:Indicators and Reagents; D017953:Isocyanates; D012834:Silver; D006046:Gold