title: Evidence of HIV-1 adaptation to host HLA alleles following chimp-to-human transmission.
abstract: BACKGROUND
  The cytotoxic T-lymphocyte immune response is important in controlling HIV-1 replication in infected humans. In this immune pathway, viral peptides within infected cells are presented to T-lymphocytes by the polymorphic human leukocyte antigens (HLA). HLA alleles exert selective pressure on the peptide regions and immune escape mutations that occur at some of the targeted sites can enable the virus to adapt to the infected host. The pattern of ongoing immune escape and reversion associated with several human HLA alleles has been studied extensively. Such mutations revert upon transmission to a host without the HLA allele because the escape mutation incurs a fitness cost. However, to-date there has been little attempt to study permanent loss of CTL epitopes due to escape mutations without an effect on fitness.
  
  
  RESULTS
  Here, we set out to determine the extent of adaptation of HIV-1 to three well-characterized HLA alleles during the initial exposure of the virus to the human cytotoxic immune responses following transmission from chimpanzee. We generated a chimpanzee consensus sequence to approximate the virus sequence that was initially transmitted to the human host and used a method based on peptide binding affinity to HLA crystal structures to predict peptides that were potentially targeted by the HLA alleles on this sequence. Next, we used codon-based phylogenetic models to quantify the average selective pressure that acted on these regions during the period immediately following the zoonosis event, corresponding to the branch of the phylogenetic tree leading to the common ancestor of all of the HIV-1 sequences. Evidence for adaptive evolution during this period was observed at regions recognised by HLA A*6801 and A*0201, both of which are common in African populations. No evidence of adaptive evolution was observed at sites targeted by HLA-B*2705, which is a rare allele in African populations.
  
  
  CONCLUSIONS
  Our results suggest that the ancestral HIV-1 virus experienced a period of positive selective pressure due to immune responses associated with HLA alleles that were common in the infected human population. We propose that this resulted in permanent escape from immune responses targeting unconstrained regions of the virus.
authors.affiliation: National Bioinformatics Node, Institute of Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory 7925, South Africa. nobubelo@cbio.uct.ac.za
authors.forename: Nobubelo K
authors.initials: NK
authors.lastname: Ngandu
authors.forename: Cathal
authors.initials: C
authors.lastname: Seoighe
authors.forename: Konrad
authors.initials: K
authors.lastname: Scheffler
mesh_terms: D000220:Adaptation, Biological; D000483:Alleles; D000818:Animals; D003062:Codon; D003198:Computer Simulation; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D002679:Pan troglodytes; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000276:immunology*
chemical_list: D003062:Codon; D006680:HLA Antigens