Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex.
The amphiphilic alpha-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 degrees C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.
Kuriyama
Shinichi
S
Pharmaceutical Research Center, Mochida Pharmaceutical Co. Ltd, Gotemba 412-8524, Japan.
Taguchi
Yasushi
Y
Watanabe
Kazuto
K
Nishimura
Kanako
K
Yanagibashi
Kazutoshi
K
Katayama
Yoshiki
Y
Niidome
Takuro
T
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism*; D004705:Endocytosis; D005615:Freezing; D000882:Haplorhini; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D013048:Specimen Handling; D014162:Transfection / Q000379:methods*; D014709:Vero Cells
D002352:Carrier Proteins; D004337:Drug Carriers; C546689:Td3717 protein; D004247:DNA