Enhanced proliferation capacity of porcine tenocytes in low O2 tension culture.
Tenocytes reside in relatively avascular tissue and are difficult to expand due to phenotype drift and functional loss. Thus low O(2) tension culture was employed to enhance the expansion capability. The results demonstrated that low O(2) tension (2% O(2)) culture could significantly enhance the expansion of newborn pig tenocytes with 275-473% greater cell yield per cell passage that that of regular O(2) cultured (21% O(2)) cells. Importantly, low O(2) culture did not change the gene expression of functional molecule such as collagens I and III, decorin, prolyl 4-hydroxylase (P4H), lysyl oxidase (LOX), TIMP-1 and TIMP-2, but could significantly down regulate the gene expression of MMP-1 and IL-6. In conclusion, low O(2) tension culture can significantly enhance the expansion capacity of tenocytes without affecting their phenotype and functions.
Zhang
Yanjie
Y
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
Wang
Bin
B
Zhang
Wen Jie
WJ
Zhou
Guangdong
G
Cao
Yilin
Y
Liu
Wei
W
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D010100:Oxygen / Q000378:metabolism*; D013552:Swine; D013710:Tendons / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D010100:Oxygen