Apolipoprotein A-I inhibits chemotaxis, adhesion, activation of THP-1 cells and improves the plasma HDL inflammatory index.
The anti-inflammatory effects of high density lipoprotein (HDL) are well described, however, such effects of Apolipoprotein A-I (ApoA-I) are less studied. Building on our previous study, we further explored the mechanism of anti-inflammatory effects of ApoA-I, and focused especially on the interaction between monocyte and endothelial cells and plasma HDL inflammatory index in LPS-challenged rabbits. Our results show that ApoA-I significantly decreased LPS-induced MCP-1 release from THP-1 cells and ox-LDL-induced THP-1 migration ratio (P<0.01, respectively). ApoA-I significantly decreased sL-selectin, sICAM-1 and sVCAM-1 release (P<0.01, P<0.01, P<0.05, respectively) from LPS-stimulated THP-1 cells. Furthermore, ApoA-I significantly inhibited LPS-induced CD11b and VCAM-1 expression on THP-1 cells (P<0.01, P<0.05, respectively). ApoA-I diminished LPS-induced mCD14 expression (P<0.01) and NFkappaB nuclear translocation in THP-1 cells. After single dose treatment of ApoA-I, the value of plasma HDL inflammatory index in LPS-challenged rabbits was improved significantly (P<0.05). These results suggest that ApoA-I can inhibit chemotaxis, adhesion and activation of human monocytes and improve plasma HDL inflammatory index with presenting beneficial anti-inflammatory effects.
Wang
Li
L
Department of Biochemistry, School of Pharmacy, Fudan University, Shanghai 200032, China.
Chen
Wei-Zhong
WZ
Wu
Man-Ping
MP
D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D002633:Chemotaxis / Q000187:drug effects*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation
D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D016328:NF-kappa B