Capillary electrochromatography with contactless conductivity detection for the determination of some inorganic and organic cations using monolithic octadecylsilica columns.
A fast separation of alkali and alkaline earth metal cations and ammonium was carried out by capillary electrochromatography on monolithic octadecylsilica columns of 15 cm length and 100 microm inner diameter using water/methanol mixtures containing acetic acid as mobile phase. On-column contactless conductivity detection was used for quantification of these non-UV-absorbing species. The method was also extended successfully to the determination of small amines as well as of amino acids, and the separation selectivity was optimized by varying the composition of the mobile phase. Detection limits of about 1 microM were possible for the inorganic cations as well as for the small amines, while the amino acids could be quantified down to about 10 microM. The separation of 12 amino acids was achieved in the relatively short time of 10 min.
Mai
Thanh Duc
TD
Centre for Environmental Technology and Sustainable Development, Hanoi University of Science, Nguyen Trai Street 334, Hanoi, Viet Nam.
Pham
Hung Viet
HV
Hauser
Peter C
PC
D019342:Acetic Acid / Q000737:chemistry; D000588:Amines / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D053801:Capillary Electrochromatography / Q000379:methods*; D002412:Cations / Q000032:analysis; D004553:Electric Conductivity; D004563:Electrochemistry; D008094:Lithium / Q000737:chemistry*; D008672:Metals, Alkali / Q000032:analysis*; D008673:Metals, Alkaline Earth / Q000032:analysis*; D000432:Methanol / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D000588:Amines; D000596:Amino Acids; D002412:Cations; D008672:Metals, Alkali; D008673:Metals, Alkaline Earth; D000644:Quaternary Ammonium Compounds; C026679:octadecylsilica; D014867:Water; D012822:Silicon Dioxide; D008094:Lithium; D019342:Acetic Acid; D000432:Methanol