title: Distinct differentiation properties of human dental pulp cells on collagen, gelatin, and chitosan scaffolds.
abstract: OBJECTIVE
  The growth and differentiation properties of human dental pulp cells (HDPC) were investigated on a variety of natural scaffolds, including 2 types of collagen, gelatin, and chitosan.
  
  
  METHODS
  Cell attachment and growth rates of HDPC on collagen (type I and type III), gelatin, and chitosan were observed. Alkaline phosphatase (ALP) activity, mRNA expression of differentiation-related genes, and mineralization of the HDPC on each scaffold were assessed.
  
  
  RESULTS
  Dental pulp cells attached and proliferated rapidly on collagen and gelatin, but chitosan did not properly support cell growth. The cells plated on gelatin exhibited high ALP activity, but not as high as cells plated on collagen. The expression peak of osteocalcin (OCN) mRNA from cells grown on collagen was found earlier and followed by dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP-1) mRNA expression. In cells grown on gelatin, however, OCN mRNA transcripts appeared at a later period of culture with no increase in DSPP or DMP-1 mRNA. Intensely mineralized extracellular matrix was seen in cells grown on collagen, but gelatin did not allow enough mineralization of cells in differentiation-inducing media.
  
  
  CONCLUSIONS
  Collagen supported proliferation and differentiation of HDPC, and the expression of DSPP and DMP-1 mRNA was reduced on gelatin.
authors.affiliation: Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul Korea.
authors.forename: Na Ryoung
authors.initials: NR
authors.lastname: Kim
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Pill-Hoon
authors.initials: PH
authors.lastname: Chung
authors.forename: Hyeong-Cheol
authors.initials: HC
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D002113:Calcification, Physiologic; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D048271:Chitosan; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D024022:Fibrillar Collagens; D005780:Gelatin; D015870:Gene Expression; D006801:Humans; D015675:Osteocalcin / Q000096:biosynthesis; D010750:Phosphoproteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012795:Sialoglycoproteins; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D000099311:Dentin Sialophosphoprotein
chemical_list: D048271:Chitosan; D016326:Extracellular Matrix Proteins; D024022:Fibrillar Collagens; D005780:Gelatin; D015675:Osteocalcin; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human