[The influence of coronary stenting with rapamycin-eluting stents on the number of circulating CD4+CD25high+regulatory T-cells].
OBJECTIVE
To investigate the effects of coronary stenting with rapamycin-eluting stents on parameters of cell immunity.


METHODS
26 patients (group 1) with stable coronary heart disease and angiographically proved coronary stenosis underwent stenting with rapamycin-eluting stents. The control group (group 2) consisted of 6 patients: 4 patients underwent diagnostic coronaroangiography, I patient got a bare metal stent and in 1 patient angioplasty was unsuccessful. Blood samples were obtained before and 1 month after the intervention. The quantity of activated (CD4+CD25low+) and regulatory (CD4+CD25high+) T cells was measured by direct immunofluorescence and flow cytometry. Plasma concentration of IL-10 was determined by ELISA.


RESULTS
In group 1 the percentages of CD4+CD25high+ regulatory T-cells increased significantly one month after stenting, while in group 2 no difference in regulatory T-cell levels before and after the intervention was observed. No changes in total number of leukocytes, relative levels of lymphocytes, CD4+ T-cells, activated CD4+CD25+low T-cells and IL-10 plasma concentration before and after the procedure were detected in both groups.


CONCLUSIONS
Rapamycin-eluting stent implantation is associated with a significant increase of circulating CD4+CD25high+ regulatory T-cell level.
Aref'eva
T I
TI
Provatorov
S I
SI
Potekhina
A V
AV
Sokolov
V O
VO
Kukhtina
N B
NB
Samko
A N
AN
Krasnikova
T L
TL
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001772:Blood Cell Count; D003327:Coronary Disease / Q000188:drug therapy / Q000276:immunology* / Q000601:surgery; D003331:Coronary Vessels / Q000187:drug effects / Q000473:pathology; D054855:Drug-Eluting Stents; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D008875:Middle Aged; D020123:Sirolimus / Q000008:administration & dosage*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D000903:Antibiotics, Antineoplastic; D016753:Interleukin-10; D020123:Sirolimus