Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction.
Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.
Kim
Seon-Soo
SS
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Byun
Hyun-Jung
HJ
Kim
Sang-Hoon
SH
Lee
Han-Hyoung
HH
Lee
Suk Jun
SJ
Kim
Sang Joon
SJ
Park
Chung-Gyu
CG
Chun
Taehoon
T
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology*; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D012995:Solubility; D013552:Swine; D000093322:Lymphocyte Activation Gene 3 Protein
D000910:Antibodies, Heterophile; D015703:Antigens, CD; D000093322:Lymphocyte Activation Gene 3 Protein