LC/MS analysis of cellular RNA reveals NAD-linked RNA.
We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of approximately 3,000 copies per cell.
Chen
Y Grace
YG
Howard Hughes Medical Institute and the Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
Kowtoniuk
Walter E
WE
Agarwal
Isha
I
Shen
Yinghua
Y
Liu
David R
DR
D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D013058:Mass Spectrometry; D009243:NAD / Q000302:isolation & purification* / Q000378:metabolism; D012329:RNA, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D012331:RNA, Fungal / Q000302:isolation & purification* / Q000378:metabolism; D012343:RNA, Transfer / Q000302:isolation & purification* / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism
D012329:RNA, Bacterial; D012331:RNA, Fungal; D009243:NAD; D012343:RNA, Transfer