Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera beta-glucosidase genes.
Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and beta-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an alpha-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and beta-glucosidase was able to produce ethanol from beta-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.
Jeon
Eugene
E
School of Life Science and Biotechnology, Korea University, Seoul 136-701, Korea.
Hyeon
Jeong-Eun
JE
Suh
Dong Jin
DJ
Suh
Young-Woong
YW
Kim
Seoung Wook
SW
Song
Kwang Ho
KH
Han
Sung Ok
SO
D002480:Cellulase / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism*; D048013:Acetivibrio thermocellus / Q000201:enzymology*; D000431:Ethanol / Q000138:chemical synthesis*; D005947:Glucose / Q000378:metabolism; D030781:Organisms, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012442:Saccharomycopsis / Q000201:enzymology*; D001617:beta-Glucosidase / Q000235:genetics*
D000431:Ethanol; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose