title: Podosomes are present in a postsynaptic apparatus and participate in its maturation.
abstract: A critical step in synapse formation is the clustering of neurotransmitter receptors in the postsynaptic membrane, directly opposite the nerve terminal. At the neuromuscular junction, a widely studied model synapse, acetylcholine receptors (AChRs) initially aggregate to form an ovoid postsynaptic plaque. As the synapse matures, the plaque becomes perforated and is eventually transformed into a complex, branched structure. We found that this transformation also occurs in myotubes cultured in the absence of neurons, and used this system to seek machinery that orchestrates postsynaptic maturation. We show that perforations in the AChR aggregate bear structures resembling podosomes, dynamic actin-rich adhesive organelles involved in matrix remodeling in non-neuronal cells but not described in neural structures. The location and dynamics of synaptic podosomes are spatiotemporally correlated with changes in AChR aggregate topology, and pharmacological disruption of podosomes leads to rapid alterations in AChR organization. Our results indicate that synaptic podosomes play critical roles in maturation of the postsynaptic membrane.
authors.affiliation: Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Tomasz J
authors.initials: TJ
authors.lastname: Proszynski
authors.forename: Jacinthe
authors.initials: J
authors.lastname: Gingras
authors.forename: Gregorio
authors.initials: G
authors.lastname: Valdez
authors.forename: Konrad
authors.initials: K
authors.lastname: Krzewski
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Sanes
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004705:Endocytosis; D005109:Extracellular Matrix / Q000378:metabolism; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism*; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000199:Actins; D011950:Receptors, Cholinergic