[Adaptation of mycoplasmas to stressful factors: nucleotide sequences nonregistered in vegetative forms of M. galisepticum S6 cells are revealed in nonculturable forms of the mycoplasma].
Adaptation of M. gallisepticum S6 to unfavorable environmental conditions is connected with transformation of the vegetative forms of the mycoplasma cells to the viable but non-culturable (VBNC) forms. The vegetative forms and the VBNC forms differ in the spectrum of the PCR-products that was forming due to amplification of the nucleotide sequence of the pvpA-gene coding the able cytoadhesion protein. As to vegetative forms of the mycoplasma the only amplicon, containing one open reading frame (1086 b.p.) with a high homology (97%) to the pvpA-gene of M. gallisepticum R and Pendik is detected. In the case of VBNC forms of M. gallisepticum S6, the additional amplicons besides those indicating the pvpA-gene of the mycoplasma are observed. In the nucleotide sequences of the additional amplicons, the open reading frames are detected that are not registered in the database of the complete sequence of the mycoplasma genome. A high homology (54-55%) of the nucleotide sequences of the pvpA-gene and the additional pvpA-amplicons allows to suggest that thepvpA-gene sequence seems to be a basis for forming new regions within the mycoplasma genome during adaptation of the bacterium to unfavorable environmental conditions.
Chernov
V M
VM
Chernova
O A
OA
Gorshkov
O V
OV
Muzykantov
A A
AA
D000220:Adaptation, Biological / Q000235:genetics*; D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D045686:Mycoplasma gallisepticum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*
D018829:Adhesins, Bacterial