[C3d-M28 enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus].
We studied the immunogenicity of pseudorabies virus gC DNA vaccination by fusing the murine complement C3d receptor binding domain. First, pseudorabies virus gC gene was linked to four copies of C3d receptor binding domain (M284), and then cloned into the vector pcDNA3.1 to construct the recombinant plasmid sgC-M284. Through the experiment of immunized BALB/c mice, we found that the enzyme linked immunosorbent assay (ELISA) antibody titer for sgC-M284 was 17-fold higher than that for sgC alone, and protective rate of mice was augmented from 25% to 88% after lethal dose PrV (316 LD50) challenge. In addition, the IL-4 levels for sgC-M284 immunization approached that for the pseudorabies virus inactivated vaccine. In conclusion, we demonstrated murine C3d receptor binding domain fusion significantly increased Th2-biased immune response by inducing IL-4 production.
Fan
Huiying
H
College of Veterinary Medicine, South China Agricultural University, Guangzhou 528427, China.
Liu
Zhongyong
Z
Tong
Tiezhu
T
Liu
Xing
X
Guo
Aizhen
A
D000276:Adjuvants, Immunologic / Q000502:physiology; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D001665:Binding Sites; D003001:Cloning, Molecular; D015933:Complement C3d / Q000235:genetics* / Q000276:immunology; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D022421:Pseudorabies Vaccines / Q000276:immunology*; D017464:Receptors, Complement 3d / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D013552:Swine; D019444:Vaccines, DNA / Q000276:immunology; D014759:Viral Envelope Proteins / Q000494:pharmacology*; D014760:Viral Fusion Proteins / Q000276:immunology
D000276:Adjuvants, Immunologic; D022421:Pseudorabies Vaccines; D017464:Receptors, Complement 3d; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C042966:pseudorabies virus glycoproteins; D015847:Interleukin-4; D015933:Complement C3d