[Selection of DNA aptamers, specifically interacting with fibrillar form of the yeast Sup35 protein].
Single-stranded DNA aptamers interacting with fibers formed by the Sup35 protein of Saccharomyces cerevisiae were obtained by the SELEX procedure. Specificity of interaction with Sup35p is investigated for 10 from total of 40 selected aptamers. It is shown that 9 aptamers bind to fibrillar Sup35p and not to monomeric form of the protein. The rate of dissociation constant of aptamer-fiber complex varies from 0.1 to 1 microM for different aptamers. Selected aptamers can be used to study prionization of Sup35.
Surina
E R
ER
Morozkina
E V
EV
Marchenko
E V
EV
Ter-Avanesian
M D
MD
Benevolenskiĭ
S V
SV
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D010454:Peptide Termination Factors / Q000737:chemistry*; D011328:Prions / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins