title: Simultaneous determination of 15 steroids in rat blood via gas chromatography-mass spectrometry to evaluate the impact of emasculation on adrenal.
abstract: Adrenal was believed to affect the prostate tumor tissue growth by its secretion of adrenal androgens. However, the mechanisms regulating these effects were not fully understood. In this work, a sensitive and specific method for the determination of 15 steroids in blood via gas chromatography-mass spectrometry in selective ion storage detection mode was established to evaluate the impact of emasculation on adrenal steroids metabolism. Steroids were isolated by solid-phase extraction using Oasis HLB cartridge, and then derivated with heptafluorobutyric anhydride before analysis. The limits of detection were between 0.15 and 1.0 ng/mL and limits of quantification were between 0.62 and 2.6 ng/mL. The recoveries of steroids were above 83%, and both the intra-day and inter-day precisions (RSD%) were lower than 8%. Pregnenolone, progesterone, 17alpha-hydroxyprogesterone (17alphaP), 17alpha-hydroxypregnenolone (17alphaH), dehydroepiandrosterone (DHEA), estrone, 17beta-estradiol, dihydrotestosterone (DHT), testosterone (T), 4-androstenedione (4-A), 1,4-androstadiene-3,17-dione, 11-deoxycortisol, 11-deoxycorticosterone, cortisol and aldosterone were quantified in 156 major male SD rats at 0, 1, 2, 4, 7 days, 2, 4, 6, 8, 10, 12, 14, and 16 weeks following emasculation. T and DHT decreased by 86.2% and 73.4%, respectively in the first 7 days following emasculation, but adrenal androgens (DHEA, 4-A) stabled at the normal level accordingly. Adrenal androgens and their precursors (17alphaH, 17alphaP) increased from the 2nd week along with the increase of androgens and the decrease of mineralocorticoids. These facts revealed that adrenal possibly enhanced its function of producing adrenal androgens from the 2nd week responding to the low androgens level induced by emasculation.
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Liang
authors.forename: Haiyin
authors.initials: H
authors.lastname: Hua
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.forename: Guo'an
authors.initials: G
authors.lastname: Luo
authors.forename: Mingqiu
authors.initials: M
authors.lastname: Hu
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Na
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000450:Aldosterone / Q000032:analysis / Q000097:blood / Q000378:metabolism; D000728:Androgens / Q000032:analysis / Q000097:blood / Q000378:metabolism; D000818:Animals; D004970:Estrone / Q000032:analysis / Q000097:blood / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006854:Hydrocortisone / Q000032:analysis / Q000097:blood / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D009919:Orchiectomy; D011284:Pregnenolone / Q000032:analysis / Q000097:blood / Q000378:metabolism; D011374:Progesterone / Q000032:analysis / Q000097:blood / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D052617:Solid Phase Microextraction; D013256:Steroids / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D013739:Testosterone / Q000032:analysis / Q000097:blood / Q000378:metabolism
chemical_list: D000728:Androgens; D013256:Steroids; D004970:Estrone; D013739:Testosterone; D000450:Aldosterone; D011374:Progesterone; D011284:Pregnenolone; D006854:Hydrocortisone