Qualea parviflora Mart.: an integrative study to validate the gastroprotective, antidiarrheal, antihemorragic and mutagenic action.
BACKGROUND
Qualea parviflora Mart. is a medicinal species commonly found in the Brazilian Cerrado biome.


OBJECTIVE
Based on ethnopharmacological data, methanolic extract from Qualea parviflora (QP) bark was evaluated for its antiulcer, analgesic, anti-hemorrhagic, mutagenic and anti-Helicobacter pylori activities.


METHODS
The gastroprotective action of the extract was evaluated in rodent experimental models (HCl/ethanol, ethanol or NSAID). We also evaluated mutagenic effect (Ames assay), anti-Helicobacter pylori, anti-hemorrhagic action, analgesic and inflammatory effects (hot-plate test and carrageenin-induced hind paw edema) of methanolic extract from Qualea parviflora.


RESULTS
QP (500 mg/kg, p.o.) was able to protect gastric mucosa against HCl/ethanol solution (77%), absolute ethanol (97%), and also against injurious effect of NSAID (36%). When QP was challenged with sulfhydryl depletor compound, the gastroprotective action of extract was abolished. QP treatment was able to maintain the GSH level and show a concentration-dependent inhibition effect on the lipid peroxidation. QP present anti-Helicobacter pylori effect (MIC=75 microg/mL), anti-hemorrhagic and antidiarrheal action but not present analgesic or anti-inflammatory effect.


CONCLUSIONS
methanolic extract from Qualea parviflora had gastroprotective effect related to the increase of gastric mucosa defensive factors such PGE(2) levels and maintain the basal gastric glutathione levels. The methanolic extract also showed anti-Helicobacter pylori activity, anti-hemorrhagic effect and antioxidant action, but absence of analgesic, mutagenic and toxic effects, a profile that adds safety to its use.
Mazzolin
Lucilene Patrícia
LP
Physiology Department, Biosciences Institute, São Paulo State University-UNESP, cp 510, Zip Code 18618-000 Botucatu, SP, Brazil.
Nasser
Ana Lúcia Martiniano
AL
Moraes
Thiago Mello
TM
Santos
Raquel Cássia
RC
Nishijima
Catarine Massucato
CM
Santos
Fábio Vieira
FV
Varanda
Eliana Aparecida
EA
Bauab
Taís Maria
TM
da Rocha
Lúcia Regina Machado
LR
Di Stasi
Luis Cláudio
LC
Vilegas
Wagner
W
Hiruma-Lima
Clélia Akiko
CA
D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000930:Antidiarrheals / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D016480:Helicobacter pylori / Q000187:drug effects / Q000502:physiology; D006490:Hemostatics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar
D000897:Anti-Ulcer Agents; D000930:Antidiarrheals; D006490:Hemostatics; D010936:Plant Extracts