Real-time voltammetric detection of cocaine-induced dopamine changes in the striatum of freely moving mice.
In the present voltammetric study, we have characterized cocaine-induced changes in evoked dopamine release and uptake in the striatum of freely moving mice in real time. Cocaine induced marked dopamine uptake inhibition measured as apparent K(m) changes, producing a maximal effect 20min following a single injection (15mg/kg, i.p.). Changes in uptake were paralleled by increases in evoked dopamine release per stimulus pulse, revealing a high correlation between these two parameters following cocaine administration. This initial characterization of cocaine effects on striatal dopamine transmission in the commonly used C57BL/6 mouse strain provides a basis for future voltammetric studies using genetic mouse models.
Oleson
Erik B
EB
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, United States.
Salek
Jonathan
J
Bonin
Keith D
KD
Jones
Sara R
SR
Budygin
Evgeny A
EA
D000818:Animals; D003042:Cocaine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D055664:Electrochemical Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D003042:Cocaine; D004298:Dopamine