Improved production of homo-D-lactic acid via xylose fermentation by introduction of xylose assimilation genes and redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-Lactate dehydrogenase gene-deficient Lactobacillus plantarum.
The production of optically pure d-lactic acid via xylose fermentation was achieved by using a Lactobacillus plantarum NCIMB 8826 strain whose l-lactate dehydrogenase gene was deficient and whose phosphoketolase genes were replaced with a heterologous transketolase gene. After 60 h of fermentation, 41.2 g/liter of d-lactic acid was produced from 50 g/liter of xylose.
Okano
Kenji
K
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
Yoshida
Shogo
S
Yamada
Ryosuke
R
Tanaka
Tsutomu
T
Ogino
Chiaki
C
Fukuda
Hideki
H
Kondo
Akihiko
A
D005285:Fermentation; D007218:Industrial Microbiology; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis* / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D014994:Xylose / Q000378:metabolism*
D019344:Lactic Acid; D014994:Xylose; D007770:L-Lactate Dehydrogenase