title: Regulated expression of the Ras effector Rin1 in forebrain neurons.
abstract: The Ras effector Rin1 is induced concomitant with synaptogenesis in forebrain neurons, where it inhibits fear conditioning and amygdala LTP. In epithelial cells, lower levels of Rin1 orchestrate receptor endocytosis. A 945 bp Rin1 promoter fragment was active in hippocampal neurons and directed accurate tissue-specific and temporal expression in transgenic mice. Regulated expression in neurons and epithelial cells was mediated in part by Snail transcriptional repressors: mutation of a conserved Snail site increased expression and endogenous Snai1 was detected at the Rin1 promoter. We also describe an element closely related to, but distinct from, the consensus site for REST, a master repressor of neuronal genes. Conversion to a consensus REST sequence reduced expression in both cell types. These results provide insight into regulated expression of a neuronal Ras effector, define a promoter useful in telencephalic neuron studies, and describe a novel REST site variant directing expression to mature neurons.
authors.affiliation: Department of Biological Chemistry, Molecular Biology Institute and Jonsson Cancer Center, David Geffen School of Medicine at UCLA, 615 Charles E. Young Drive, 34609 BSRB, Los Angeles, CA 90095-1737, USA.
authors.forename: Bartholomew
authors.initials: B
authors.lastname: Dzudzor
authors.forename: Lucia
authors.initials: L
authors.lastname: Huynh
authors.forename: Minh
authors.initials: M
authors.lastname: Thai
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Bliss
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Toh Hean
authors.initials: TH
authors.lastname: Ch'ng
authors.forename: Meisheng
authors.initials: M
authors.lastname: Jiang
authors.forename: Kelsey C
authors.initials: KC
authors.lastname: Martin
authors.forename: John
authors.initials: J
authors.lastname: Colicelli
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011401:Promoter Regions, Genetic; D016548:Prosencephalon / Q000166:cytology* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C490451:Rin1 protein, mouse; C000604407:SNAI1 protein, human; C000604429:Snai1 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D020691:rab GTP-Binding Proteins