Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.
We established a novel method, insertional chromatin immunoprecipitation (iChIP), for isolation of specific genomic regions. In iChIP, specific genomic domains are immunoprecipitated with antibody against a tag, which is fused to the DNA-binding domain of an exogenous DNA-binding protein, whose recognition sequence is inserted into the genomic domains of interest. The iChIP method will be a useful tool for dissecting chromatin structure of genomic region of interest.
Hoshino
Akemi
A
Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
Fujii
Hodaka
H
D001483:Base Sequence; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods*; D016678:Genome / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics* / Q000379:methods*