Rab6 and Rab11 regulate Chlamydia trachomatis development and golgin-84-dependent Golgi fragmentation.
Many intracellular pathogens that replicate in special membrane bound compartments exploit cellular trafficking pathways by targeting small GTPases, including Rab proteins. Members of the Chlamydiaceae recruit a subset of Rab proteins to their inclusions, but the significance of these interactions is uncertain. Using RNA interference, we identified Rab6 and Rab11 as important regulators of Chlamydia infections. Depletion of either Rab6 or Rab11, but not the other Rab proteins tested, decreased the formation of infectious particles. We further examined the interplay between these Rab proteins and the Golgi matrix components golgin-84 and p115 with regard to Chlamydia-induced Golgi fragmentation. Silencing of the Rab proteins blocked Chlamydia-induced and golgin-84 knockdown-stimulated Golgi disruption, whereas Golgi fragmentation was unaffected in p115 depleted cells. Interestingly, p115-induced Golgi fragmentation could rescue Chlamydia propagation in Rab6 and Rab11 knockdown cells. Furthermore, transport of nutrients to Chlamydia, as monitored by BODIPY-Ceramide, was inhibited by Rab6 and Rab11 knockdown. Taken together, our results demonstrate that Rab6 and Rab11 are key regulators of Golgi stability and further support the notion that Chlamydia subverts Golgi structure to enhance its intracellular development.
Rejman Lipinski
Anette
A
Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany.
Heymann
Julia
J
Meissner
Charlotte
C
Karlas
Alexander
A
Brinkmann
Volker
V
Meyer
Thomas F
TF
Heuer
Dagmar
D
D001766:Blindness / Q000453:epidemiology / Q000382:microbiology; D002455:Cell Division; D002690:Chlamydia Infections / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism / Q000635:transmission; D002692:Chlamydia trachomatis / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000382:microbiology; D005260:Female; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D015994:Incidence; D007247:Infertility, Female / Q000382:microbiology; D034622:RNA Interference; D012749:Sexually Transmitted Diseases / Q000453:epidemiology / Q000635:transmission; D020691:rab GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
C094135:Rab6 protein; D020691:rab GTP-Binding Proteins