Dual response surface-optimized synthesis of L-menthyl conjugated linoleate in solvent-free system by Candida rugosa lipase.
Lipase-catalyzed synthesis of L-menthyl conjugated linoleate in solvent-free system was studied in this paper. Duel response surface methodology was employed to explore the factors which would influence the reaction conversion by a range of independent experiments. The conditions of reaction temperature, reaction time, enzyme amount, substrate molar ratio and water content were symmetrically investigated. When the substrates were 1 mmol CLA and 1 mmol L-menthol, the maximum conversion (79.1+/-0.8%) was obtained at 30 degrees C, Candida rugosa lipase of 33.7% (w/w by weight of L-menthol), water content of 32% (w/w by weight of L-menthol), reaction time of 43 h. The product isomers (9Z,11E-/10E,12Z-=63/37) were analyzed by GC/MS. The similarity between the oleic acid and 9Z,11E-CLA conformations which were obtained by molecular modeling could account for the specific catalyzed by C. rugosa lipase.
Li
Zhen
Z
School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, PR China.
Wang
Yulun
Y
Li
Jia
J
Wang
Ping
P
Wei
Wei
W
Gao
Yang
Y
Fu
Chenyin
C
Dong
Wenqi
W
D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D008049:Lipase / Q000378:metabolism*; D008610:Menthol / Q000737:chemistry*; D008956:Models, Chemical; D008968:Molecular Conformation; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry*; D013499:Surface Properties
D012997:Solvents; D008610:Menthol; D019787:Linoleic Acid; D008049:Lipase