title: Osteogenic induction of adipose-derived stromal cells: not a requirement for bone formation in vivo.
abstract: Osteogenic induction was regarded as an indispensable step for adipose-derived stromal cells (ADSCs) to have osteogenic ability. Non-induced ADSCs can also produce bone in vivo and heal skeletal defects. The present study aimed to compare the bone-forming ability of osteogenically induced ADSCs and non-induced ADSCs in vivo. Tissue-engineered constructs were prepared from osteogenically induced or non-induced ADSCs and porous hydroxyapatite/beta-tricalcium phosphate scaffolds. A scaffold without cells and an empty defect group were used as control. All were implanted in rat critical calvarial defects. After implantation for 6 and 12 weeks, bone formation was analyzed using histomorphometry and microcomputed tomography; there were no significant differences in the formation of new bone between osteogenically induced ADSCs and non-induced ADSCs (P > 0.05). In conclusion, osteogenic induction of ADSCs is not an indispensable step for bone formation in vivo. Non-induced ADSCs can also be used as seeding cells to construct bone tissue.
authors.affiliation: Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Yao
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Jing
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lin
authors.forename: Weidong
authors.initials: W
authors.lastname: Tian
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001842:Bone and Bones / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D049452:Green Fluorescent Proteins; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000293:injuries / Q000473:pathology; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D049452:Green Fluorescent Proteins