title: A general life-death selection strategy for dissecting protein functions.
abstract: Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.
authors.affiliation: Département de Biochimie, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Po Hien
authors.initials: PH
authors.lastname: Ear
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Michnick
mesh_terms: D043525:Cytosine Deaminase / Q000235:genetics*; D005816:Genetic Complementation Test / Q000379:methods; D010446:Peptide Fragments / Q000378:metabolism; D025941:Protein Interaction Mapping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development
chemical_list: D010446:Peptide Fragments; D043525:Cytosine Deaminase