[Analysis of serum metabonome of patients with breast cancer by gas chromatography-mass spectrometry].
To analyze serum metabonome of patients with breast carcinoma by gas chromatography-mass spectrometry (GC-MS).



Serum samples from 30 patients with breast carcinoma and 25 normal people were collected. Endogenous metabolites amino acids, fatty acids, saccharides and organic acids were analyzed after derivatization.



The injection precision and intra-day precision (RSD) were less than 14.4 %. The method displayed good recovery (80.7 %-118.0 % with RSD<12.0 %) and linearity (r(2)>0.9905). Samples were stable in 24 h at room temperature. The samples were stable through three freeze-thaw cycles (RSD<14.6 %). Alaine,oxalate and glutamine were found with frequency of 96.7 %, 100 % and 93.3 %, respectively, which indicated that they may be the potential biomarkers.



The method is stable and reliable, which may be helpful for the diagnosis of breast cancer as an auxiliary method.
Wang
Jin
J
Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China.
Yu
Lan-Fang
LF
Shen
Peng
P
Wang
Shu-Fang
SF
D000328:Adult; D001943:Breast Neoplasms / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D044967:Serum / Q000737:chemistry / Q000378:metabolism*