title: Quantitative measurement of aging using image texture entropy.
abstract: BACKGROUND
  A key element in understanding the aging of Caenorhabditis elegans is objective quantification of the morphological differences between younger and older animals. Here we propose to use the image texture entropy as an objective measurement that reflects the structural deterioration of the C. elegans muscle tissues during aging.
  
  
  RESULTS
  The texture entropy and directionality of the muscle microscopy images were measured using 50 animals on Days 0, 2, 4, 6, 8, 10 and 12 of adulthood. Results show that the entropy of the C. elegans pharynx tissues increases as the animal ages, but a sharper increase was measured between Days 2 and 4, and between Days 8 and 10. These results are in agreement with gene expression findings, and support the contention that the process of C. elegans aging has several distinct stages. This can indicate that C. elegans aging is driven by developmental pathways, rather than stochastic accumulation of damage.
  
  
  BACKGROUND
  The image data are freely available on the Internet at http://ome.grc.nia.nih.gov/iicbu2008/celegans, and the Haralick and Tamura texture analysis source code can be downloaded at http://ome.grc.nia.nih.gov/wnd-charm.
authors.affiliation: Laboratory of Genetics, National Institute on Aging/NIH, 251 Bayview Boulevard, Baltimore, MD 21224, USA. shamirl@mail.nih.gov
authors.forename: Lior
authors.initials: L
authors.lastname: Shamir
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Wolkow
authors.forename: Ilya G
authors.initials: IG
authors.lastname: Goldberg
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D016208:Databases, Factual; D019277:Entropy; D017238:Genes, Helminth; D008136:Longevity
chemical_list: D029742:Caenorhabditis elegans Proteins