FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction.
A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.
Nonaka
Hiroshi
H
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
Fujishima
Sho-hei
SH
Uchinomiya
Sho-hei
SH
Ojida
Akio
A
Hamachi
Itaru
I
D001665:Binding Sites; D008958:Models, Molecular; D015394:Molecular Structure; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling
D009842:Oligopeptides; D010455:Peptides; D011506:Proteins; C085706:FLAG peptide