title: [The apoptosis of mouse macrophage J774A.1 induced by oxysterol depend on NF-kappaB activation].
abstract: OBJECTIVE
  Investigated the relationship between NF-kappaB activation and cell apoptosis in mouse macrophages treated with 7-ketochesterol (7-KC).
  
  
  METHODS
  Cell apoptosis was detected by MTT assay, DNA fragmentation assay and flow cytometric analysis. NF-kappaB activation was detected by western blot and immunohistochemistry. Inhibitory assay was used to show the effect of the activation of NF-kappaB on the apoptosis induced by 7-KC.
  
  
  RESULTS
  7-KC inhibited macrophages proliferation, and then induced apoptosis, which is associated with NF-kappaB activation. Moreover, cell apoptosis with NF-kappaB activation was inhibited by pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-kappaB.
  
  
  CONCLUSIONS
  7-KC induced the activation of NF-kappaB and following cell apoptosis.
authors.affiliation: Key Laboratory of Bio-organic Chemistry, College of Bioengineering, Dalian University, Dalian 116622, China. zhenyu_bio@hotmail.com
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Huang
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Liu
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Li
authors.forename: Ren-Jun
authors.initials: RJ
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Chi
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.forename: Qi-Ming
authors.initials: QM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D007653:Ketocholesterols / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D007653:Ketocholesterols; D016328:NF-kappa B; C003001:7-ketocholesterol