Comparisons of recombinant resilin-like proteins: repetitive domains are sufficient to confer resilin-like properties.
Two novel recombinant proteins An16 and Dros16 have recently been generated. These recombinant proteins contain, respectively, sixteen copies of an 11 amino acid repetitive domain (AQTPSSQYGAP) observed in a resilin-like gene from Anopheles gambiae and sixteen copies of a 15 amino acid repetitive domain (GGRPSDSYGAPGGGN) observed in the first exon of the Drosophila melanogaster CG15920 gene. We compare structural characteristics of the proteins and material properties of resulting biopolymers relative to Rec1-resilin, a previously characterized resilin-like protein encoded by the first exon of the Drosophila melanogaster CG15920 gene. While the repetitive domains of natural resilins display significant variation both in terms of amino acid sequence and length, our synthetic polypeptides have been designed as perfect repeats. Using techniques including circular dichroism, atomic force microscopy, and tensile testing, we demonstrate that both An16 and Dros16 have similar material properties to those previously observed in insect and recombinant resilins. Modulus, elasticity, resilience, and dityrosine content in the cross-linked biomaterials were assessed. Despite the reduced complexity of the An16 and Dros16 proteins compared to natural resilins, we have been able to produce elastic and resilient biomaterials with similar properties to resilin.
Lyons
Russell E
RE
CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, QLD, Australia. Russell.Lyons@csiro.au
Nairn
Kate M
KM
Huson
Mickey G
MG
Kim
Misook
M
Dumsday
Geoff
G
Elvin
Christopher M
CM
D000595:Amino Acid Sequence; D000818:Animals; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics; D020724:Elastomers / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics
D020724:Elastomers; D019476:Insect Proteins; D011994:Recombinant Proteins; C030951:resilin