Production of oxygenated fatty acids from vegetable oils by Flavobacterium sp. strain DS5.
Flavobacterium sp. strain DS5 (NRRL B-14859) was used to convert two vegetable oils, olive oil and soybean oil, directly to oxygenated fatty acids such as 10-ketostearic acid (10-KSA) and 10-hydroxystearic acid (10-HSA). Lipase addition to the culture was required because strain DS5 did not induce lipase activity to release free fatty acids from vegetable oils. 10-KSA production was higher from olive oil than from soybean oil because olive oil contains more oleic acid, the precursor of 10-KSA. The optimum amounts of olive oil and lipase addition for 10-KSA production were determined as 0.3ml and 1mg (specific activity=700units/mg) per 50ml culture medium, respectively. At these conditions, 2.8g/L of 10-KSA and 0.40g/L of 10-HSA were obtained from olive oil as a substrate.
Heo
Shin-Haeng
SH
Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Hou
Ching T
CT
Kim
Beom Soo
BS
D002849:Chromatography, Gas; D003470:Culture Media; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D005417:Flavobacterium / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*
D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D010938:Plant Oils; D008049:Lipase; D010100:Oxygen