Seed-conjugated polymer bead for beta2-microglobulin removal at neutral pH.
beta2-Microglobulin (beta2m) is known to be a major factor for dialysis-related amyloidosis. We have studied beta2m removal through an aggregation process, which was initiated by a ligand that could catch the beta2m monomer and promote its aggregation into fibril. As a ligand, we have prepared beta2m fibril fragments and used them as a seed. The seed was coupled to PEGylated-PS beads to remove the monomeric beta2m from solution. The beta2m seed-conjugated resin effectively adsorbed the beta2m monomers with a capacity of 3.6 mg/ml via promoting their aggregation into fibrils on the resin at pH 7.4.
Kim
Mira
M
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea.
Kang
Sungsoo
S
Myung
Eun-Kyung
EK
Ahn
Minkoo
M
Choi
Jeong-Hyun
JH
Paik
Seung R
SR
Lee
Yoon-Sik
YS
D006801:Humans; D006863:Hydrogen-Ion Concentration; D007676:Kidney Failure, Chronic / Q000378:metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D011487:Protein Conformation; D012639:Seeds / Q000737:chemistry*; D012996:Solutions; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D012996:Solutions; D001613:beta 2-Microglobulin