title: Nondigestible saccharides suppress the bacterial degradation of quercetin aglycone in the large intestine and enhance the bioavailability of quercetin glucoside in rats.
abstract: Contribution of intestinal bacterial degradation of quercetin aglycone to the promotive effects of fructooligosaccharides and di-D-fructose anhydride III (DFAIII) on quercetin-3-O-beta-glucoside (Q3G) bioavailability was examined. Male Sprague-Dawley rats were fed 0.68% Q3G diets with or without 1.5% or 3% oligosaccharides for 2 weeks. Blood levels and urinary excretion of quercetin and methylquercetin conjugates, measured by methanol extraction and LC-MS analyses, were dose-dependently and adaptively increased by the oligosaccharide supplementation with increasing cecal fermentation (Experiment 1). Degradation of Q3G and quercetin aglycone by cecal bacteria in oligosaccharide-fed rats was much lower than that in the control rats using an anaerobic culture system (Experiment 2). Using the ligated intestinal sacs of anesthetized rats, we found that the cecum possessed high absorptive capacity for quercetin derivatives (Experiment 3). These results demonstrate that feeding of the oligosaccharides strongly suppresses the bacterial degradation of quercetin aglycone in the cecum, thus largely contributing to the increased bioavailability of Q3G.
authors.affiliation: Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Matsukawa
authors.forename: Megumi
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Aki
authors.initials: A
authors.lastname: Shinoki
authors.forename: Masahito
authors.initials: M
authors.lastname: Hagio
authors.forename: Ryo
authors.initials: R
authors.lastname: Inoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D001682:Biological Availability; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000378:metabolism; D015536:Down-Regulation; D007420:Intestine, Large / Q000378:metabolism / Q000382:microbiology*; D008297:Male; D009844:Oligosaccharides / Q000008:administration & dosage / Q000378:metabolism*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004040:Dietary Carbohydrates; D009844:Oligosaccharides; C016527:isoquercitrin; D011794:Quercetin