Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells.
StAPs are potato aspartic proteases with cytotoxic activity against plant pathogens and spermatozoa. StAPs cytotoxic activity is selective, since these proteins do not exert toxic effect on plant cells and erythrocytes. In this work, we investigated the capacity of StAPs to exert cytotoxicity on human leukaemia cells. Obtained results show that StAPs induce apoptosis on Jurkat T cells after a short time of incubation in a dose-dependent manner. However, no significative effect on the T lymphocytes viability was observed at all StAPs incubation times and concentrations tested. These results suggest that StAPs can be conceptually promising leads for cancer therapy.
Mendieta
Julieta R
JR
Institute of Biological Research, University of Mar del Plata, Mar del Plata, Argentina.
Fimognari
Carmela
C
Daleo
Gustavo R
GR
Hrelia
Patrizia
P
Guevara
María G
MG
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D057055:Aspartic Acid Proteases / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects; D015458:Leukemia, T-Cell / Q000188:drug therapy*; D008517:Phytotherapy; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D035281:Plant Tubers; D011198:Solanum tuberosum / Q000737:chemistry*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D010940:Plant Proteins; D057055:Aspartic Acid Proteases