title: Proteomic analysis of the secretome of human umbilical vein endothelial cells using a combination of free-flow electrophoresis and nanoflow LC-MS/MS.
abstract: Human umbilical vein endothelial cells are the most widely used in vitro model for endothelial cells. Their secreted proteins, however, have not been comprehensively analysed so far. In this study, we accomplished to map the secretome of human umbilical vein endothelial cells by combining free-flow electrophoresis with nanoflow LC-MS/MS. This comprehensive analysis provides a basis for future comparative studies of protein secretion by endothelial cells in response to cardiovascular risk factors and is available on our website http://www.vascular-proteomics.com.
authors.affiliation: Pharmacology Laboratory (CDB), Hospital Clinic, IDIBAPS, Barcelona University, CIBERehd, Barcelona, Spain.
authors.forename: David Guillen
authors.initials: DG
authors.lastname: Tunica
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Yin
authors.forename: Anissa
authors.initials: A
authors.lastname: Sidibe
authors.forename: Christin
authors.initials: C
authors.lastname: Stegemann
authors.forename: Mikkel
authors.initials: M
authors.lastname: Nissum
authors.forename: Lingfang
authors.initials: L
authors.lastname: Zeng
authors.forename: Merce
authors.initials: M
authors.lastname: Brunet
authors.forename: Manuel
authors.initials: M
authors.lastname: Mayr
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D042783:Endothelial Cells / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014471:Umbilical Veins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins