Protein kinase CK2 regulates cytoskeletal reorganization during ionizing radiation-induced senescence of human mesenchymal stem cells.
Human mesenchymal stem cells (hMSC) are critical for tissue regeneration. How hMSC respond to genotoxic stresses and potentially contribute to aging and cancer remain underexplored. We showed that ionizing radiation induced cellular senescence of hMSC over a period of 10 days, showing a critical transition between days 3 and 6. This was confirmed by senescence-associated beta-galactosidase staining, protein expression profiles of key cell cycle regulators (retinoblastoma protein, p53, p21(waf1/Cip1), and p16(INK4A)), and senescence-associated secretory phenotypes (interleukin-8, interleukin-12, GRO, and MDC). We observed dramatic cytoskeletal reorganization of hMSC through reduction of myosin-10, redistribution of myosin-9, and secretion of profilin-1. Using a SILAC-based phosphoproteomics method, we detected significant reduction of myosin-9 phosphorylation at Ser(1943), coinciding with its redistribution. Importantly, through treatment with cell-permeable inhibitors (4,5,6,7-tetrabromo-1H-benzotriazole and 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole) and gene knockdown using RNA interference, we identified CK2, a kinase responsible for myosin-9 phosphorylation at Ser(1943), as a key factor contributing to the radiation-induced senescence of hMSC. We showed that individual knockdown of CK2 catalytic subunits CK2alpha and CK2alpha' induced hMSC senescence. However, only knockdown of CK2alpha resulted in morphologic phenotypes resembling those of radiation-induced senescence. These results suggest that CK2alpha and CK2alpha' play differential roles in hMSC senescence progression, and their relative expression might represent a novel regulatory mechanism for CK2 activity.
Wang
Daojing
D
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. djwang@lbl.gov
Jang
Deok-Jin
DJ
D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D047390:Casein Kinase II / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016922:Cellular Senescence / Q000528:radiation effects*; D016207:Cytokines / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000201:enzymology / Q000528:radiation effects*; D010641:Phenotype; D010750:Phosphoproteins; D010766:Phosphorylation; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology; D011839:Radiation, Ionizing; D055815:Young Adult
D015415:Biomarkers; D016207:Cytokines; D010750:Phosphoproteins; D020543:Proteome; D034741:RNA, Small Interfering; D047390:Casein Kinase II