Improved rapid assay of plasma uric acid by short-end injection capillary zone electrophoresis.
A rapid and simple short-end injection capillary zone electrophoresis method was developed for the quantification of plasma uric acid. The separation was performed in an uncoated fused-silica capillary (50 microm ID, 60 cm total length, 10.2 cm effective length) by using as a background electrolyte a 75 mmol/L glycylglycine solution titrated with NaOH 5 mol/L to pH 9.0, a voltage of 28 kV, a cartridge temperature of 15 degrees C, and direct UV detection at 292 nm. Under optimized conditions, uric acid was determinate in little more than 1 min (1.076 minutes). In order to verify the accuracy of the analysis, urate levels were measured in 543 apparently healthy volunteers by the new assay and our previous method, and the obtained data were compared by Passing-Bablock regression, Bland-Altman test, and a new regression-based approach, which showed a good agreement between two methods.
Sotgia
Salvatore
S
Department of Biomedical Sciences and Centre of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Viale San Pietro 43, 07100, Sassari, Italy. salvatore.sotgia@gmail.com
Carru
Ciriaco
C
Scanu
Bastianina
B
Pisanu
Elisabetta
E
Sanna
Manuela
M
Pinna
Gerard Aime
GA
Gaspa
Leonardo
L
Deiana
Luca
L
Zinellu
Angelo
A
D001681:Biological Assay / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D014527:Uric Acid / Q000097:blood
D014527:Uric Acid