Evaluation of oxidant-antioxidant balance in patients on maintenance haemodialysis: a comparative study of dialyzers membranes.
BACKGROUND
Haemodialysis (HD) exacerbates oxidative stress (OS). The polymethyl-methacrylate (PMMA)-BK-F membrane ameliorates OS and inflammation markers compared to polyacrylonitrile (PAN/AN69) and cellulose membranes. This may be due to the size of pore radius, high flux or other specific properties of PMMA membranes.


OBJECTIVE
To compare OS and inflammatory status in HD-treated end stage renal disease patients with membranes of different pore size radius and flux.


METHODS
47 patients of both sexes were studied. The HD membranes with which the patients were normally treated were changed to BK-P or B-3 membranes for 6 months. Intracellular and extracellular components of the oxidant-antioxidant balance (OAB), C-reactive protein (CRP), beta2-micro-globulin (beta2mu-globulin), albumin and transferrin were measured.


RESULTS
A significant decrease in red cell membrane thiobarbituric acid reacting substances and an increase in cytosolic superoxide dismutase (SOD) and plasma total antioxidant substances were observed in all patients after 6 months of treatment with BK-P and B-3 membranes except SOD and CRP in patients previously dialysed with triacetate cellulose membranes. Albumin and transferrin remained unmodified. beta2mu-globulin significantly decreased after treatment with PMMA membranes.


CONCLUSIONS
BK-P and B-3 HD membranes improved the OAB, beta2mu-globulin and CRP compared to PAN/AN69 and cellulose diacetate membranes.
Macías Núñez
Juan F
JF
Hospital Clínico Universitario, Salamanca, Spain. jfmacias@usal.es
Ghais
Zoubiair
Z
Bustamante
Jesus
J
Lopez-Novoa
Jose M
JM
Arrieta
J
J
Barril
G
G
Del Pino
María D
MD
Escaja
C
C
Martín
I M
IM
Fernández Andrade
C
C
García
F
F
Núñez
J
J
Onaindía
J M
JM
González
J
J
Pérez-Prieto
M
M
Fernández
M
M
Lopez
J
J
Santiago
J M
JM
Vicens
M
M
Sousa
F
F
D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000032:analysis; D015897:Comorbidity; D004867:Equipment Design; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000453:epidemiology / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D006435:Renal Dialysis; D012709:Serum Albumin / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D014168:Transferrin / Q000032:analysis; D001613:beta 2-Microglobulin / Q000097:blood
D008567:Membranes, Artificial; D012709:Serum Albumin; D014168:Transferrin; D001613:beta 2-Microglobulin; D002097:C-Reactive Protein; D013482:Superoxide Dismutase