Surface characterization of extracellular matrix scaffolds.
Extracellular matrix (ECM) scaffolds prepared from different tissue sources or using different methods have been demonstrated to have distinctive effects upon cell adhesion patterns and the ability to support and maintain differentiated phenotypes. It is unknown whether the molecular composition or the ultrastructure of the ECM plays a greater role in determining the phenotype of the cells with which it comes into contact. However, when implanted, the topology and ligand landscape of the material will determine the host molecules that bind and the type and behavior of cells that mediate the host response. Therefore, a comprehensive understanding of surface characteristics is essential in the design of scaffolds for specific clinical applications. The surface characteristics of ECM scaffolds derived from porcine urinary bladder, small intestine, and liver as well as the effects of two commonly used methods of chemical cross-linking upon UBM were investigated. Electron microscopy and time of flight secondary ion mass spectroscopy were used to examine the surface characteristics of the scaffolds. The results show that ECM scaffolds have unique morphologic and structural properties which are dependant on the organ or tissue from which the scaffold is harvested. Furthermore, the results show that the surface characteristics of an ECM scaffold are changed through chemical cross-linking.
Brown
Bryan N
BN
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Barnes
Christopher A
CA
Kasick
Rena T
RT
Michel
Roger
R
Gilbert
Thomas W
TW
Beer-Stolz
Donna
D
Castner
David G
DG
Ratner
Buddy D
BD
Badylak
Stephen F
SF
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology; D008099:Liver / Q000166:cytology; D013058:Mass Spectrometry / Q000379:methods; D008422:Materials Testing; D010641:Phenotype; D025341:Principal Component Analysis; D013499:Surface Properties; D013552:Swine; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D001743:Urinary Bladder / Q000166:cytology
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents