Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis.
Mycoplasma pneumoniae is a leading cause of community-acquired pneumonia. Although two genetically distinct types of M. pneumoniae are known, variants of each also exist. We used a real-time PCR high-resolution melt genotyping assay to identify clinical variants which may provide greater insight into the genetic distribution of M. pneumoniae strains.
Schwartz
Stephanie B
SB
Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Mitchell
Stephanie L
SL
Thurman
Kathleen A
KA
Wolff
Bernard J
BJ
Winchell
Jonas M
JM
D018829:Adhesins, Bacterial / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000453:epidemiology / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D044366:Transition Temperature
D018829:Adhesins, Bacterial; C035471:adhesin, Mycoplasma pneumoniae