title: Establishment and conventional cytogenetic characterization of three gastric cancer cell lines.
abstract: Gastric cancer is the fourth most frequent type of cancer and the second most frequent cause of cancer mortality worldwide. Only a modest number of gastric carcinoma cell lines have been isolated thus far. Here we describe the establishment and cytogenetic characterization of three new gastric cancer cell lines obtained from primary gastric adenocarcinoma (ACP02 and ACP03) and cancerous ascitic fluid (AGP01) of individuals from northern Brazil. ACP02, ACP03, and AGP01 cell lines are presently in the 60th passage. The cell lines grew in a disorganized single layer with some agglomerations and heterogeneous divisions (bipolar and multipolar). All cell lines exhibited a composite karyotype with several clonal chromosome alterations. Trisomy 8 was the most frequent alteration. Chromosome 8 aneusomy was confirmed by fluorescence in situ hybridization. All cell lines also exhibited trisomy 7 and deletion of chromosome arm 17p. These results suggest that, although frequent chromosome alterations are commonly observed due to culture process, the ACP02, ACP03, and AGP01 cell lines and primary gastric cancer from individuals of northern Brazil share genetic alterations, supporting use of these cell lines as a model of gastric carcinogenesis in this population.
authors.affiliation: Genetics Division, Department of Morphology and Genetics, Federal University of São Paulo, Ed Leitão da Cunha, São Paulo, CEP 04023-900 São Paulo, SP, Brazil. mariana.morf@epm.br
authors.forename: Mariana Ferreira
authors.initials: MF
authors.lastname: Leal
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Martins do Nascimento
authors.forename: Carla Elvira Araújo
authors.initials: CE
authors.lastname: da Silva
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Vita Lamarão
authors.forename: Danielle Queiroz
authors.initials: DQ
authors.lastname: Calcagno
authors.forename: André Salim
authors.initials: AS
authors.lastname: Khayat
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: Assumpção
authors.forename: Isabel Rosa
authors.initials: IR
authors.lastname: Cabral
authors.forename: Marília
authors.initials: M
authors.lastname: de Arruda Cardoso Smith
authors.forename: Rommel Rodríguez
authors.initials: RR
authors.lastname: Burbano
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D045744:Cell Line, Tumor; D020732:Cytogenetic Analysis; D006801:Humans; D008297:Male; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology