Biopure MTAD Induces DNA Damage but Not Cellular Death: An In Vitro Study.
OBJECTIVE
The purpose of this study was to evaluate the capacity of BioPure MTAD to induce genetic damage in vitro. Genotoxicity was assessed by the single cell gel (comet) assay.


METHODS
Chinese hamster ovary (CHO) cells or murine fibroblasts cells were exposed to increasing final concentrations ranging from 0.1 a 10%. All treatments were performed for 1 hour at 37 degrees C. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 1 hour at 37 degrees C and the positive control group was treated with methylmetanesulfonate (at 1 muM) for 1 hour at 37 degrees C.


RESULTS
Present results showed that the BioPure MTAD was able to promote DNA breakage in CHO cells only at the highest concentration tested as well as to induce significant increase in tail moment at all tested concentrations in murine fibroblasts.


CONCLUSIONS
In summary, our results indicate that BioPure MTAD is a genotoxic agent as depicted by the single cell gel (comet) assay. (Eur J Dent 2009;3:285-289).
Marins
Juliana Soares Roter
JS
Department of Endodontics, School of Dentistry, Rio de Janeiro State University, UERJ, Rio de Janeiro RJ, Brazil.
Sassone
Luciana Moura
LM
Ribeiro
Daniel Araki
DA