title: Environmental monitoring of Domingo Rubio stream (Huelva Estuary, SW Spain) by combining conventional biomarkers and proteomic analysis in Carcinus maenas.
abstract: Element load, conventional biomarkers and altered protein expression profiles were studied in Carcinus maenas crabs, to assess contamination of "Domingo Rubio" stream, an aquatic ecosystem that receives pyritic metals, industrial contaminants, and pesticides. Lower antioxidative activities - glucose-6-phosphate and 6-phosphogluconate dehydrogenases, catalase - were found in parallel to higher levels of damaged biomolecules - malondialdehyde, oxidized glutathione -, due to oxidative lesions promoted by contaminants, as the increased levels of essential - Zn, Cu, Co - and nonessential - Cr, Ni, Cd - elements. Utility of Proteomics to assess environmental quality was confirmed, especially after considering the six proteins identified by de novo sequencing through capLC-muESI-ITMS/MS and homology search on databases. They include tripartite motif-containing protein 11 and ATF7 transcription factor (upregulated), plus CBR-NHR-218 nuclear hormone receptor, two components of the ABC transporters and aldehyde dehydrogenase (downregulated). These proteins could be used as novel potential biomarkers of the deleterious effects of pollutants present in the area.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba, Severo Ochoa Building, Rabanales Campus, Highway A4 Km 396a, 14071 Córdoba, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Montes Nieto
authors.forename: Tamara
authors.initials: T
authors.lastname: García-Barrera
authors.forename: José-Luis
authors.initials: JL
authors.lastname: Gómez-Ariza
authors.forename: Juan
authors.initials: J
authors.lastname: López-Barea
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D003386:Brachyura / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods* / Q000706:statistics & numerical data*; D020869:Gene Expression Profiling / Q000379:methods / Q000706:statistics & numerical data; D005880:Gills / Q000737:chemistry; D008861:Microsomes / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D045483:Rivers / Q000737:chemistry*; D013030:Spain; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical