title: Expression quantitative trait loci are highly sensitive to cellular differentiation state.
abstract: Genetical genomics is a strategy for mapping gene expression variation to expression quantitative trait loci (eQTLs). We performed a genetical genomics experiment in four functionally distinct but developmentally closely related hematopoietic cell populations isolated from the BXD panel of recombinant inbred mouse strains. This analysis allowed us to analyze eQTL robustness/sensitivity across different cellular differentiation states. Although we identified a large number (365) of "static" eQTLs that were consistently active in all four cell types, we found a much larger number (1,283) of "dynamic" eQTLs showing cell-type-dependence. Of these, 140, 45, 531, and 295 were preferentially active in stem, progenitor, erythroid, and myeloid cells, respectively. A detailed investigation of those dynamic eQTLs showed that in many cases the eQTL specificity was associated with expression changes in the target gene. We found no evidence for target genes that were regulated by distinct eQTLs in different cell types, suggesting that large-scale changes within functional regulatory networks are uncommon. Our results demonstrate that heritable differences in gene expression are highly sensitive to the developmental stage of the cell population under study. Therefore, future genetical genomics studies should aim at studying multiple well-defined and highly purified cell types in order to construct as comprehensive a picture of the changing functional regulatory relationships as possible.
authors.affiliation: Department of Cell Biology, Section Stem Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
authors.forename: Alice
authors.initials: A
authors.lastname: Gerrits
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Tesson
authors.forename: Leonid V
authors.initials: LV
authors.lastname: Bystrykh
authors.forename: Ellen
authors.initials: E
authors.lastname: Weersing
authors.forename: Albertina
authors.initials: A
authors.lastname: Ausema
authors.forename: Bert
authors.initials: B
authors.lastname: Dontje
authors.forename: Xusheng
authors.initials: X
authors.lastname: Wang
authors.forename: Rainer
authors.initials: R
authors.lastname: Breitling
authors.forename: Ritsert C
authors.initials: RC
authors.lastname: Jansen
authors.forename: Gerald
authors.initials: G
authors.lastname: de Haan
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers