A comparison of HPLC/APCI-MS and MALDI-MS for characterising triacylglycerols in insects: species-specific composition of lipids in the fat bodies of bumblebee males.
Two mass spectrometric methods for analysing triacylglycerols (HPLC/APCI-MS and MALDI-MS) were used and compared in terms of the relevance of the data for further biostatistical evaluation. While MALDI-MS is simpler and significantly faster, the time-consuming and labour-intensive HPLC/APCI-MS provides more complete information about the lipid components. However, both methods provide well-comparable results concerning the grouping of specimens belonging to different species when evaluated with multivariate exploratory approaches. The compositions of triacylglycerols in the fat bodies of males in 11 bumblebee species (Bombus terrestris, B. lucorum, B. lapidarius, B. pratorum, B. sylvarum, B. ruderatus, B. pomorum, B. subterraneus, B. campestris, B. bohemicus, and B. rupestris) were found to be species-specific.
Kofronová
Edita
E
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovo nám. 2, 166 10 Prague, Czech Republic; Institute of Chemical Technology, Technická 5, 166 28 Prague, Czech Republic.
Cvacka
Josef
J
Vrkoslav
Vladimír
V
Hanus
Robert
R
Jiros
Pavel
P
Kindl
Jirí
J
Hovorka
Oldrich
O
Valterová
Irena
I
D000818:Animals; D001516:Bees / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005216:Fat Body / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014280:Triglycerides / Q000737:chemistry*
D014280:Triglycerides