[Sequence and evolutionary analysis of VP1 gene of ovine rotavirus NT].
OBJECTIVE
The ovine rotavirus strain NT isolated from diarrhea lamb in China was considered as a promising vaccine strain. Based on the VP1 gene was one of the important structural proteins of rotavirus, we studied on the evolutional characteristics of VP1.


METHODS
According to the published conservative sequences of VP1 genes, we designed a pair of specific primers for cloning and sequencing of VP1 gene.


RESULTS
Sequencing result showed that the VP1 gene was 3,302 bp in length and the deduced protein was 1,088 aa. Comparison of amino acid sequences revealed that the ORV-NT shared 77.3% - 98.4% similarities with other group A rotaviruses. Furthermore, sequence alignment analysis manifested that amino acid variations mainly occurred in the non-functional regions of VP1 protein. Phylogenetic analysis of VP1 protein showed that the OVR-NT was grouped in the bovine rotavirus clusters, indicating a closer relationship between them. Evolutionary distance of nucleotide sequence and amino acid sequence among VP1 genes of different rotaviruses were calculated, respectively. Analysis of synonymous mutation rate and Non-synonymous mutation rate demonstrated that synonymous substitution was the major pattern of variation in the process of evolution.


CONCLUSIONS
This was the first report on sequencing and evolutionary distance analysis of VP1 gene of ORV-NT.
Chen
Yanjun
Y
Key Lab of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China. chenyj20@163.com
Zhu
Weiwen
W
Sui
Shuo
S
Zhang
Xiaowei
X
Hu
Songnian
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics*
C069187:VP1 protein, Rotavirus; D014758:Viral Core Proteins