Quantitative plasma biomarker analysis in HDI exposure assessment.
Quantification of amines in biological samples is important for evaluating occupational exposure to diisocyanates. In this study, we describe the quantification of 1,6-hexamethylene diamine (HDA) levels in hydrolyzed plasma of 46 spray painters applying 1,6-hexamethylene diisocyanate (HDI)-containing paint in vehicle repair shops collected during repeated visits to their workplace and their relationship with dermal and inhalation exposure to HDI monomer. HDA was detected in 76% of plasma samples, as heptafluorobutyryl derivatives, and the range of HDA concentrations was < or =0.02-0.92 microg l(-1). After log-transformation of the data, the correlation between plasma HDA levels and HDI inhalation exposure measured on the same workday was low (N = 108, r = 0.22, P = 0.026) compared with the correlation between plasma HDA levels and inhalation exposure occurring approximately 20 to 60 days before blood collection (N = 29, r = 0.57, P = 0.0014). The correlation between plasma HDA levels and HDI dermal exposure measured on the same workday, although statistically significant, was low (N = 108, r = 0.22, P = 0.040) while the correlation between HDA and dermal exposure occurring approximately 20 to 60 days before blood collection was slightly improved (N = 29, r = 0.36, P = 0.053). We evaluated various workplace factors and controls (i.e. location, personal protective equipment use and paint booth type) as modifiers of plasma HDA levels. Workers using a downdraft-ventilated booth had significantly lower plasma HDA levels relative to semi-downdraft and crossdraft booth types (P = 0.0108); this trend was comparable to HDI inhalation and dermal exposure levels stratified by booth type. These findings indicate that HDA concentration in hydrolyzed plasma may be used as a biomarker of cumulative inhalation and dermal exposure to HDI and for investigating the effectiveness of exposure controls in the workplace.
Flack
Sheila L
SL
Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, Rosenau Hall, NC 27599, USA.
Fent
Kenneth W
KW
Trelles Gaines
Linda G
LG
Thomasen
Jennifer M
JM
Whittaker
Steve
S
Ball
Louise M
LM
Nylander-French
Leena A
LA
D000395:Air Pollutants, Occupational / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D000418:Albumins / Q000032:analysis; D001336:Automobiles; D015415:Biomarkers / Q000097:blood; D003485:Cyanates / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D003627:Data Interpretation, Statistical; D003959:Diamines / Q000097:blood*; D004784:Environmental Monitoring; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D019570:Inhalation Exposure / Q000032:analysis*; D017953:Isocyanates; D016014:Linear Models; D008297:Male; D016273:Occupational Exposure / Q000032:analysis*; D010150:Paint; D011482:Protective Devices / Q000706:statistics & numerical data; D012869:Skin Absorption; D013997:Time Factors; D017132:Workplace / Q000592:standards
D000395:Air Pollutants, Occupational; D000418:Albumins; D015415:Biomarkers; D003485:Cyanates; D003959:Diamines; D006454:Hemoglobins; D017953:Isocyanates; C015262:1,6-hexamethylene diisocyanate; C007297:1,6-diaminohexane