The nonsynonymous single-nucleotide polymorphisms in codon 31 of p21 gene and the susceptibility to cervical cancer in Chinese women.
BACKGROUND
It was suggested that single-nucleotide polymorphisms in p21 codon 31 seem to be associated with a variety of human malignancies; very few studies have focused on the association between p21 codon 31 polymorphisms and cervical cancer. This study explored whether p21 codon 31 nonsynonymous single-nucleotide polymorphisms might be associated with an increased risk of cervical cancer development among Chinese women.


METHODS
Peripheral blood samples were obtained from patients with cervical cancer (n = 317) and healthy controls (n = 353) for detecting the biallelic polymorphisms at codon 31 of p21 gene by the mismatch amplification mutation assay-polymerase chain reaction. Cervix brush-off samples were obtained from patients with cervical squamous cell carcinoma (SCC) and controls for detection of high-risk human papillomavirus (HR-HPV).


RESULTS
The AGA (Arg) allele frequency in patients with cervical SCCs was significantly higher than that in controls. AGA/AGA and AGA/AGC genotypes were more frequently found in cervical SCCs than in controls. There was no significant difference of allele frequency or genotype distribution between cervical adenocarcinomas and controls, or between HR-HPV-positive and HR-HPV-negative groups.


CONCLUSIONS
p21 Codon 31 with AGA (Arg) allele is a genetic risk factor of cervical SCC, and the increased risk is probably not caused by increasing host susceptibility to HR-HPV infection.
Tian
Qifang
Q
Department of Gynecologic Oncology, Women's Reproductive Health Laboratory, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Lu
Weiguo
W
Chen
Huaizeng
H
Ye
Feng
F
Xie
Xing
X
D000230:Adenocarcinoma / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000235:genetics*; D016022:Case-Control Studies; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D020125:Mutation, Missense / Q000502:physiology; D030361:Papillomavirus Infections / Q000150:complications; D020641:Polymorphism, Single Nucleotide; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000235:genetics*
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21