title: [Conserved structural features of ETS domain--DNA complexes].
abstract: ETS proteins comprise a family of widespread transcription factors regulating expression of many animal genes. Structurally ETS proteins are characterized by conserved DNA-binding ETS domain, recognizing DNA sequences with trinucleotide GGA. Comparative analysis of structural features of ETS domain-DNA complexes was carried out and conserved contacts, important in terms of interaction stability and specificity were identified. The analysis revealed 9 conserved hydrogen bonds with DNA backbone phosphates and 2 conserved bidentate hydrogen bonds with DNA major groove atoms, one conserved hydrophobic cluster on protein-DNA interface, important for binding site recognition, and 12 conserved water molecules, possibly mediating ETS domain-DNA interaction. The results are represented in specialized data bank of protein-DNA complexes NPIDB.
authors.forename: A V
authors.initials: AV
authors.lastname: Grishin
authors.forename: A V
authors.initials: AV
authors.lastname: Alekseevskiĭ
authors.forename: S A
authors.initials: SA
authors.lastname: Spirin
authors.forename: A S
authors.initials: AS
authors.lastname: Kariagin
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D050783:Proto-Oncogene Proteins c-ets / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D050783:Proto-Oncogene Proteins c-ets; D004247:DNA