title: Automated precolumn derivatization system for analyzing physiological amino acids by liquid chromatography/mass spectrometry.
abstract: An automated method for high-throughput amino acid analysis, using precolumn derivatization high-performance liquid chromatography/electrospray mass spectrometry (HPLC/ESI-MS), was developed and evaluated. The precolumn derivatization step was performed in the reaction port of a home-built auto-sampler system. Amino acids were derivatized with 3-aminopyridyl-N-hydroxysuccinimidyl carbamate, and a 3 microm Wakosil-II 3C8-100HG column (100 x 2.1 mm i.d.) was used for separation. To achieve a 13 min cycle for each sample, the derivatization and separation steps were performed in parallel. The results of the method evaluation, including the linearity, and the intra- and inter-precision, were sufficient to measure physiological amino acids in human plasma samples. The relative standard deviations of typical amino acids in actual human plasma samples were below 10%.
authors.affiliation: Institute of Life Sciences, Ajinomoto Co., Inc., 1-1 Suzuki-cho Kawasaki-ku, Kawasaki, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Shimbo
authors.forename: Shintaro
authors.initials: S
authors.lastname: Kubo
authors.forename: Yushi
authors.initials: Y
authors.lastname: Harada
authors.forename: Takashi
authors.initials: T
authors.lastname: Oonuki
authors.forename: Takefumi
authors.initials: T
authors.lastname: Yokokura
authors.forename: Hiroo
authors.initials: H
authors.lastname: Yoshida
authors.forename: Michiko
authors.initials: M
authors.lastname: Amao
authors.forename: Mina
authors.initials: M
authors.lastname: Nakamura
authors.forename: Naoko
authors.initials: N
authors.lastname: Kageyama
authors.forename: Junko
authors.initials: J
authors.lastname: Yamazaki
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ozawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hirayama
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ando
authors.forename: Junkichi
authors.initials: J
authors.lastname: Miura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyano
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D000596:Amino Acids