title: Polymorphisms of the ICAM-1 exon 6 (E469K) are associated with differentiation of colorectal cancer.
abstract: BACKGROUND
  Genetic factors are thought to play a role in development for colorectal carcinogenesis. ICAM-1 is a polymorphic gene, thus, the present study investigated the relationship between the polymorphisms of ICAM-1 and the susceptibility and phenotypical characteristics of colorectal cancer (CRC).
  
  
  METHODS
  The polymorphisms at ICAM-1 exon 4 (G241R) and exon 6 (E469K) were detected by PCR with sequence-specific primers. The relationship between specific genotypes of ICAM-1 and differentiation of CRC was evaluated by the histological grade.
  
  
  RESULTS
  We showed only GG genotype of ICAM-1 individuals in either CRC or normal controls. The KK genotype of ICAM-1 K469E was found more frequently than in the controls (P < 0.05). Patients with well-differentiated CRC displayed the KK more frequently than those of poor differentiation (P < 0.05).
  
  
  CONCLUSIONS
  The findings indicate that polymorphisms of G241R are rare in Chinese population and that KK genotype of ICAM-1 K469E is significantly associated with well differentiation of CRC.
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, China. walksweet@foxmail.com
authors.forename: Qing-lei
authors.initials: QL
authors.lastname: Wang
authors.forename: Bing-hui
authors.initials: BH
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Ya-bin
authors.initials: YB
authors.lastname: Liu
authors.forename: Yue-Ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Sui-Bing
authors.initials: SB
authors.lastname: Miao
authors.forename: Yi
authors.initials: Y
authors.lastname: Han
authors.forename: Jin-Kun
authors.initials: JK
authors.lastname: Wen
authors.forename: Mei
authors.initials: M
authors.lastname: Han
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D015153:Blotting, Western; D002454:Cell Differentiation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005091:Exons; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D018799:Intercellular Adhesion Molecule-1