Sterodin, a novel immunostimulating drug: some toxicological and pharmacological evaluations in vivo, and drug-lipid interaction studies in vitro.
Sterodin is a novel non-specific immunostimulating drug produced by a combination of bile lipids and bacterial metabolites. In the present study, we investigated some of its (i) toxicological and (ii) pharmacological properties in vivo, and (iii) drug-lipid interaction (lipid peroxidation) in vitro. We also evaluated the possible (iv) Sterodin-induced lipid peroxidation as well as the effect of ascorbic acid on this peroxidation. We found LD50 of Sterodin to be 31.50 mL kg-1 body mass. In male albino mice, Sterodin increased the total white blood cells and neutrophils count by 59 and 26%, respectively, on the 6th day, compared to day 0 after injection and stimulated phagocytic activity in vivo. We used goat liver as lipid source in drug-lipid interaction studies in vitro. Our experiments show that Sterodin induces lipid peroxidation, which was prevented by ascorbic acid.
Ray
Sarbani Dey
SD
Dr. B C Roy College of Pharmacy & Allied Health Sciences, Bidhannagar, Durgapur-713206, India.
Roy
Partha Pratim
PP
Ray
Supratim
S
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D000818:Animals; D005978:Glutathione / Q000037:antagonists & inhibitors; D006041:Goats; D007928:Lethal Dose 50; D007958:Leukocyte Count; D050356:Lipid Metabolism / Q000187:drug effects* / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008055:Lipids / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology* / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D010865:Pilot Projects
D000276:Adjuvants, Immunologic; D008055:Lipids; C549774:Sterodin; D005978:Glutathione