A new bioassay for the inspection and identification of TBT-containing antifouling paint.
Since the 1960s tributyl (TBT)-based antifouling paints are widely applied to protect ship's hulls from biofouling. Due to its high toxicity to aquatic ecosystem most of the countries (28 nations in 2008) signed the AFS convention to control the use of harmful antifouling systems on ships. Nevertheless there is currently no simple method to control the presence of organotin in paint. In this study, we propose a bioassay based on the use of a recombinant bioluminescent bacteria to detect directly in paint the presence of TBT. We also propose a simple device as an inspection system to control the absence of organotin in the ship's hull paint. The presence of organotin could be revealed in less than three hours.
Gueuné
Hervé
H
University of Nantes, UMR CNRS 6144, GEPEA, CBAC Group, IUT de Roche sur Yon, 18 bd. Gaston Defferre, 85035 La Roche sur Yon, France.
Thouand
Gérald
G
Durand
Marie-José
MJ
D001681:Biological Assay / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation*; D004926:Escherichia coli / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D009947:Organotin Compounds / Q000032:analysis; D010150:Paint / Q000032:analysis*; D012767:Ships; D014220:Trialkyltin Compounds / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D008164:Luminescent Proteins; D009947:Organotin Compounds; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C056189:di-n-butyltin; C011559:tributyltin