title: Identification of a synthetic peptide inducing cross-reactive antibodies binding to Rhipicephalus (Boophilus) decoloratus, Rhipicephalus (Boophilus) microplus, Hyalomma anatolicum anatolicum and Rhipicephalus appendiculatus BM86 homologues.
abstract: The BM86 antigen, originally identified in Rhipicephalus (Boophilus) microplus, is the basis of the only commercialized anti-tick vaccine. The long-term goal of our study is to improve BM86 based vaccines by induction of high levels of tick gut binding antibodies that are also cross-reactive with a range of BM86 homologues expressed in other important tick species. Here we have used a BD86 derived synthetic peptide, BD86-3, to raise a series of mouse monoclonal antibodies. One of these mAbs, named 12.1, recognized BM86 homologues in immuno-histochemical analyses in four out of five tick species including R. (B.) microplus, Rhipicephalus (Boophilus) decoloratus, Hyalomma anatolicum anatolicum and Rhipicephalus appendiculatus. Our results indicate that broadly cross-reactive tick gut binding antibodies can be induced after immunization with a synthetic peptide derived from the protein BD86.
authors.affiliation: Swiss Tropical Institute, Department of Medical Parasitology and Infection Biology, Socinstrasse 57, 4002 Basel, Switzerland.
authors.forename: Nadja
authors.initials: N
authors.lastname: Kopp
authors.forename: Diana
authors.initials: D
authors.lastname: Diaz
authors.forename: Mario
authors.initials: M
authors.lastname: Amacker
authors.forename: David O
authors.initials: DO
authors.lastname: Odongo
authors.forename: Konstantin
authors.initials: K
authors.lastname: Beier
authors.forename: Cordula
authors.initials: C
authors.lastname: Nitsch
authors.forename: Richard P
authors.initials: RP
authors.lastname: Bishop
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Daubenberger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006825:Hybridomas; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D048494:Rhipicephalus / Q000276:immunology; D016415:Sequence Alignment; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D022721:Vaccines, Virosome / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C085065:BM86 protein, Boophilus; D008562:Membrane Glycoproteins; D010455:Peptides; D011994:Recombinant Proteins; D014612:Vaccines; D022223:Vaccines, Subunit; D022721:Vaccines, Virosome