Isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. BR-2.
Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78%of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 16S rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCosEPA-BR2 produced EPA at a maximum level of 7.5%of total fatty acids, confirming the EPA biosynthesis activity of the cloned gene cluster.
Lee
Su-Jin
SJ
Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, Korea.
Seo
Pil-Soo
PS
Kim
Chul Ho
CH
Kwon
Ohsuk
O
Hur
Byung-Ki
BK
Seo
Jeong-Woo
JW
D000595:Amino Acid Sequence; D000818:Animals; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D005399:Fishes / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D030781:Organisms, Genetically Modified / Q000235:genetics; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020592:Shewanella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D015118:Eicosapentaenoic Acid