title: HPLC determination of ibuprofen, diclofenac and salicylic acid using hollow fiber-based liquid phase microextraction (HF-LPME).
abstract: This paper describes an extraction method using a polypropylene membrane supporting dihexyl ether (three-phase hollow fiber-based liquid phase microextraction (HF-LPME)) for the analysis of several pharmaceuticals (salicylic acid (SAC), ibuprofen (IBU) and diclofenac (DIC)) followed by a HPLC determination using a monolithic silica type HPLC column, that allows lower retention times than the usual packed columns with adequate resolution. Detection was realized by means of a coupled in series diode array (DAD) and fluorescence (FLD) detectors. HF-LPME is a relatively new technique employed in analytical chemistry for sample pretreatment which offers more selectivity and sensitivity than any traditional extraction technique. Detection limits by DAD are 12, 53 and 40 ng mL(-1) for salicylic acid, diclofenac and ibuprofen, respectively and by FLD 7 and 2 ng mL(-1) for salicylic acid, and ibuprofen. The method has been successfully applied to their direct determination in human urine and the results obtained demonstrated that could be also applied to the determination of the corresponding metabolites.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, Seville 41012, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Ramos Payán
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Bello López
authors.forename: Rut
authors.initials: R
authors.lastname: Fernández-Torres
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Pérez Bernal
authors.forename: Manuel
authors.initials: M
authors.lastname: Callejón Mochón
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000032:analysis*; D007052:Ibuprofen / Q000032:analysis*; D008567:Membranes, Artificial; D011126:Polypropylenes / Q000737:chemistry; D020156:Salicylic Acid / Q000032:analysis*; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial; D011126:Polypropylenes; D004008:Diclofenac; D020156:Salicylic Acid; D007052:Ibuprofen