Effect of Costaria costata fucoidan on expression of matrix metalloproteinase-1 promoter, mRNA, and protein.
Fucoidans are sulfated fucosylated polymers from brown algae cell walls. We assessed the inhibitory effects of Costaria costata fucoidan on UVB-induced MMP-1 promoter, mRNA, and protein expression in vitro using the immortalized human keratinocyte (HaCaT) cell line. Pretreatment with fucoidan significantly inhibited MMP-1 protein expression compared to UVB irradiation alone. Fucoidan significantly reduced MMP-1 mRNA expression and inhibited UVB-induced MMP-1 promoter activity by 37.3%, 53.3%, and 58.5% at 0.01, 0.1, and 1 microg/mL, respectively, compared to UVB irradiation alone. C. costata fucoidan may be a potential therapeutic agent to prevent and treat skin photoaging.
Moon
Hee Jung
HJ
Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University, Busan, 602-702, Korea.
Park
Kyong Sik
KS
Ku
Mi Jeong
MJ
Lee
Myeong Sook
MS
Jeong
Seok Hoon
SH
Imbs
Tatyana I
TI
Zvyagintseva
Tatyana N
TN
Ermakova
Svetlana P
SP
Lee
Yong Hwan
YH
D002473:Cell Wall / Q000737:chemistry; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects / Q000235:genetics*; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D014466:Ultraviolet Rays
D011134:Polysaccharides; D011506:Proteins; D012333:RNA, Messenger; C007789:fucoidan; D020781:Matrix Metalloproteinase 1