title: Ultrastructural evidence of amyloid beta-induced autophagy in PC12 cells.
abstract: Herein we demonstrate that PC12 cells overexpress human amyloid beta precursor protein bearing double Swedish mutation(AbetaPPsw), showing the phenotype characteristic for Alzheimer's disease (AD). Examination of cells at ultrastructural level revealed the intracellular presence of peptide aggregates. Furthermore, autophagy induction was found to be a hallmark of amyloid beta-induced cytotoxicity. Importantly, autophagic vacuoles were co-localized within amyloid beta (Abeta) deposits. This suggests the involvement of autophagy in amyloid beta-elicited cell degeneration.
authors.affiliation: Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland. bepaj@wp.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Pajak
authors.forename: Martyna
authors.initials: M
authors.lastname: Songin
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Strosznajder
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Orzechowski
authors.forename: Barbara
authors.initials: B
authors.lastname: Gajkowska
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001343:Autophagy / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D009410:Nerve Degeneration / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D016716:PC12 Cells; D051381:Rats; D014162:Transfection
chemical_list: D016564:Amyloid beta-Protein Precursor