title: Using flowerlike polymer-copper nanostructure composite and novel organic-inorganic hybrid material to construct an amperometric biosensor for hydrogen peroxide.
abstract: A new type of amperometric hydrogen peroxide biosensor was fabricated by entrapping horseradish peroxidase (HRP) in the organic-inorganic hybrid material composed of zirconia-chitosan sol-gel and Au nanoparticles (ZrO2-CS-AuNPs). The sensitivity of the biosensor was enhanced by a flowerlike polymer-copper nanostructure composite (pPA-FCu) which was prepared from co-electrodeposition of CuSO4 solution and 2,6-pyridinediamine solution. Several techniques, including UV-vis absorption spectroscopy, scanning electron microscopy, cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy were employed to characterize the assembly process and performance of the biosensor. The results showed that this pPA-FCu nanostructure not only had excellent redox electrochemical activity, but also had good catalytic efficiency for hydrogen peroxide. Also the ZrO2-CS-AuNPs had good film forming ability, high stability and good retention of bioactivity of the immobilized enzyme. The resulting biosensors showed a linear range from 7.80 x 10(-7) to 3.7 x 10(-3) mol L(-1), with a detection limit of 3.2 x 10(-7) mol L(-1) (S/N=3) under optimized experimental conditions. The apparent Michaelis-Menten constant was determined to be 0.32 mM, showing good affinity. In addition, the biosensor which exhibits good analytical performance, acceptable stability and good selectivity, has potential for practical applications.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Jinfen
authors.initials: J
authors.lastname: Wang
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
authors.forename: Ligen
authors.initials: L
authors.lastname: Min
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D015040:Zirconium / Q000737:chemistry*
chemical_list: D011108:Polymers; D003300:Copper; D048271:Chitosan; D006861:Hydrogen Peroxide; D015040:Zirconium; D006735:Horseradish Peroxidase; C028541:zirconium oxide