Hepatoprotective evaluation of Anogeissus latifolia: in vitro and in vivo studies.
OBJECTIVE
To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl(4)).


METHODS
In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl(4) and extract of Anogeissus latifolia. Hepatoprotective activity was demonstrated in the CCl(4) damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl(4)-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections.


RESULTS
In vitro: primary hepatocyte monolayer cultures were treated with CCl(4) and extract of Anogeissus latifolia. A protective activity could be demonstrated in the CCl(4) damaged primary monolayer culture. In vivo: Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl(4)-induced liver damage as judged from serum marker enzyme activity.


CONCLUSIONS
The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified.
Pradeep
Hulikere Ananth
HA
Department of Pharmacology and Biotechnology, Nizam Institute of Pharmacy, Deshmukhi, Pochampally (Mandal), Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India.
Khan
Saleemullah
S
Ravikumar
Karamkonda
K
Ahmed
Mohammed Fazil
MF
Rao
Meesala Srinivasa
MS
Kiranmai
Mandava
M
Reddy
Dachani Sudhershan
DS
Ahamed
Shaik Rasheed
SR
Ibrahim
Mohammed
M
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control; D002478:Cells, Cultured; D029764:Combretaceae / Q000378:metabolism*; D005260:Female; D022781:Hepatocytes / Q000187:drug effects*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects*; D008107:Liver Diseases / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase