title: Immunodetection of NGF, trkA, p75 and inhibin alpha-subunit in interstitial cells of golden hamsters treated with hCG.
abstract: The aim of this study was to investigate the effect of luteinizing hormone (LH) surge on expression of nerve growth factor (NGF), trkA, p75 and inhibin alpha-subunit in ovarian interstitial cells of golden hamsters after human chorionic gonadotropin (hCG) treatment. NGF, two NGF receptors (trkA, p75) and inhibin alpha-subunit were immunolocalized by immunohistochemistry, and gonadotropins, steroid hormones and immunoreactive (ir-) inhibin concentrations were measured by radioimmunoassay. Stronger positive staining of NGF, trkA and p75 were found in interstitial cells at 6, 12 and 18 h after hCG injection in the treated group compared with the control groups. Inhibin alpha-subunit staining was found in interstitial cells at 12 and 18 h after hCG injection in the treatment group, but not in the control group. Plasma concentrations of progesterone increased significantly from 6 to 18 h after hCG treatment, whereas plasma concentrations of estradiol-17beta significantly decreased compared with the control group. An increased plasma concentration of FSH and decreased concentrations of ir-inhibin from 6 to 18 h after hCG treatment verified the negative relationship. There were no significant changes in the concentrations of LH in the hCG-treated group. In contrast, the typical preovulatory LH surge was found at 1700 h on day 4 (proestrus) in the control group. These results suggested that LH surge can induce expression of NGF, trkA, p75 and inhibin alpha-subunit in ovarian interstitial cells and that NGF, trkA, p75 and inhibin alpha-subunit may have a paracrine or autocrine role in modulation of ovarian interstitial cell function in golden hamsters.
authors.affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing, China. weng3@msn.com
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.forename: ZhanQuan
authors.initials: Z
authors.lastname: Shi
authors.forename: Junko
authors.initials: J
authors.lastname: Tukada
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D006224:Cricetinae; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood; D006062:Gonadotropins / Q000097:blood; D007150:Immunohistochemistry; D007265:Inhibins / Q000097:blood / Q000378:metabolism*; D008647:Mesocricetus; D020932:Nerve Growth Factor / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010062:Ovulation Induction; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism*; D020917:Receptor, trkA / Q000378:metabolism*; D013799:Theca Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D012739:Gonadal Steroid Hormones; D006062:Gonadotropins; D020800:Receptor, Nerve Growth Factor; C094377:inhibin-alpha subunit; D007265:Inhibins; D020932:Nerve Growth Factor; D020917:Receptor, trkA