title: Engineering anatomically shaped human bone grafts.
abstract: The ability to engineer anatomically correct pieces of viable and functional human bone would have tremendous potential for bone reconstructions after congenital defects, cancer resections, and trauma. We report that clinically sized, anatomically shaped, viable human bone grafts can be engineered by using human mesenchymal stem cells (hMSCs) and a "biomimetic" scaffold-bioreactor system. We selected the temporomandibular joint (TMJ) condylar bone as our tissue model, because of its clinical importance and the challenges associated with its complex shape. Anatomically shaped scaffolds were generated from fully decellularized trabecular bone by using digitized clinical images, seeded with hMSCs, and cultured with interstitial flow of culture medium. A bioreactor with a chamber in the exact shape of a human TMJ was designed for controllable perfusion throughout the engineered construct. By 5 weeks of cultivation, tissue growth was evidenced by the formation of confluent layers of lamellar bone (by scanning electron microscopy), markedly increased volume of mineralized matrix (by quantitative microcomputer tomography), and the formation of osteoids (histologically). Within bone grafts of this size and complexity cells were fully viable at a physiologic density, likely an important factor of graft function. Moreover, the density and architecture of bone matrix correlated with the intensity and pattern of the interstitial flow, as determined in experimental and modeling studies. This approach has potential to overcome a critical hurdle-in vitro cultivation of viable bone grafts of complex geometries-to provide patient-specific bone grafts for craniofacial and orthopedic reconstructions.
authors.affiliation: Department of Biomedical Engineering, Columbia University, VC 12-234, New York, NY 10032, USA.
authors.forename: Warren L
authors.initials: WL
authors.lastname: Grayson
authors.forename: Mirjam
authors.initials: M
authors.lastname: Fröhlich
authors.forename: Keith
authors.initials: K
authors.lastname: Yeager
authors.forename: Sarindr
authors.initials: S
authors.lastname: Bhumiratana
authors.forename: M Ete
authors.initials: ME
authors.lastname: Chan
authors.forename: Christopher
authors.initials: C
authors.lastname: Cannizzaro
authors.forename: Leo Q
authors.initials: LQ
authors.lastname: Wan
authors.forename: X Sherry
authors.initials: XS
authors.lastname: Liu
authors.forename: X Edward
authors.initials: XE
authors.lastname: Guo
authors.forename: Gordana
authors.initials: G
authors.lastname: Vunjak-Novakovic
mesh_terms: D019149:Bioreactors; D016025:Bone Transplantation; D006801:Humans; D008335:Mandibular Condyle / Q000033:anatomy & histology / Q000254:growth & development / Q000637:transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D013704:Temporomandibular Joint / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*; D019737:Transplants