title: Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages.
abstract: Oxidative stress and inflammation are two of the most critical factors implicated in carcinogenesis and other degenerative disorders. We have investigated how lunasin, a known anti-cancer seed peptide, affect these factors. This peptide inhibits linoleic acid oxidation and acts as 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenger. Furthermore, using LPS-stimulated RAW 264.7 macrophages, we have demonstrated that lunasin reduces, in a significant dose-dependent manner, the production of reactive oxygen species (ROS) by LPS-induced macrophages. Lunasin also inhibits the release of pro-inflammatory cytokines (tumor necrosis factor-alpha [TNF-alpha] and interleukine-6 [IL-6]). On the basis of these potent antioxidant and anti-inflammatory properties, we propose lunasin not only as a cancer preventive and therapeutic agent but also as an agent against other inflammatory-related disorders.
authors.affiliation: Department of Nutritional Sciences and Toxicology, University of California Berkeley, CA 94720-3104, USA. Blanca@berkeley.edu
authors.forename: Blanca
authors.initials: B
authors.lastname: Hernández-Ledesma
authors.forename: Chia-Chien
authors.initials: CC
authors.lastname: Hsieh
authors.forename: Ben O
authors.initials: BO
authors.lastname: de Lumen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D019787:Linoleic Acid / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D030262:Soybean Proteins / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016588:Anticarcinogenic Agents; D000975:Antioxidants; C120514:GM2S-1 protein, soybean; D017382:Reactive Oxygen Species; D030262:Soybean Proteins; D019787:Linoleic Acid