Identification and characterization of estrogen receptor-related receptor alpha and gamma in human glioma and astrocytoma cells.
The purpose of this study was to examine expression and function of estrogen receptor-related receptors (ERRs) in human glioma and astrocytoma cell lines. These estrogen receptor-negative cell lines expressed ERRalpha and ERRgamma proteins to varying degree in a cell context dependent manner, with U87MG glioma cells expressing both orphan nuclear receptors. Cell proliferation assays were performed in the presence of ERR isoform-specific agonists and antagonists, and the calculated EC(50) and IC(50) values were consistent with previous reported values determined in other types of cancer cell lines. Induction of luciferase expression under the control of ERR isoform-specific promoters was also observed in these cells. These results indicate that ERRalpha and ERRgamma are differentially expressed in these tumor cell lines and likely contribute to agonist-dependent ERR transcriptional activity.
Gandhari
Mukesh K
MK
Laboratory of Clinical Investigation, Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA.
Frazier
Chester R
CR
Hartenstein
Julia S
JS
Cloix
Jean-Francois
JF
Bernier
Michel
M
Wainer
Irving W
IW
D001254:Astrocytoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D011401:Promoter Regions, Genetic; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D000097983:ERRalpha Estrogen-Related Receptor
D011960:Receptors, Estrogen; D000097983:ERRalpha Estrogen-Related Receptor; C490917:ESRRG protein, human