Establishing a 3D ex vivo culture system for investigations of bone metabolism and biomaterial interactions.
A 3D load-providing culture system is presented, which enables human cancellous bone explants to be studied ex vivo. Bone integrity and activity was demonstrated during 2 to 3 weeks of culture. Viable osteocytes and bone specific markers could be identified. Culture conditions were improved using a serum free medium containing TGF-beta3. Therefore this system gives the opportunity to study bone biology, hormonal effects as well as biomaterial interactions ex vivo prior to animal studies.
Richards
R Geoff
RG
AO Research Institute, Davos Platz, Switzerland. geoff.richards@aofoundation.org
Simpson
Angharad E
AE
Jaehn
Katharina
K
Furlong
Pamela I
PI
Stoddart
Martin J
MJ
D001847:Bone Diseases / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D010006:Osteoblasts / Q000166:cytology; D010010:Osteoclasts / Q000166:cytology; D010011:Osteocytes / Q000166:cytology / Q000187:drug effects; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology; D016474:Weight-Bearing
D003470:Culture Media; D053782:Transforming Growth Factor beta3