Fontibacter flavus gen. nov., sp. nov., a member of the family 'Cyclobacteriaceae', isolated from a hot spring.
The taxonomic position of a bright orange-pigmented bacterial strain, designated CC-GZM-130(T), isolated from a water sample of the Guan-zing-ling hot spring, southern Taiwan, was studied. The strain was able to grow on nutrient agar at 25-40 degrees C and in the presence of 1-3 % (w/v) NaCl. Comparative analyses of the 16S rRNA gene sequence showed that the isolate was grouped in the vicinity of the genus Aquiflexum with the highest sequence similarity of 92.1 % to the type strain of Aquiflexum balticum, followed by sequence similarities of 92.0, 91.6 and 91.5 % to the type strains of Algoriphagus ornithinivorans, Algoriphagus hitonicola and Belliella baltica, respectively. The polyamine pattern showed that the major compound was sym-homospermidine. The quinone system was menaquinone MK-7. The polar lipid profile was composed predominantly of phosphatidylethanolamine, three polar lipids and one aminolipid. Minor amounts of other lipids were also detectable. The main characteristics of the fatty acid profiles of strain CC-GZM-130(T), B. baltica and Aquiflexum balticum were similar, with iso-C(15 : 0), iso-C(17 : 1)ω 9c and iso-C(17 : 0) 3-OH as the major fatty acids, but some qualitative and quantitative differences were observed. The DNA G+C content of the novel strain was 53.2 mol%. The isolate clearly differed genotypically and phenotypically from representatives of the most closely related genera. On the basis of these differences, a novel species in a new genus, Fontibacter flavus gen. nov., sp. nov., is proposed with CC-GZM-130(T) (=CCUG 57694(T)=CCM 7650(T)) as the type strain of the type species.
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, IFZ - Heinrich-Buff-Ring 26-32, D35392 Giessen, Germany.
Young
Chiu-Chung
CC
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Rekha
P D
PD
Yenepoya Research Center, Yenepoya University, Nityanada Nagar, Deralakatte, Mangalore 575 018, Karnataka, India.
Fallschissel
Kerstin
K
Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, IFZ - Heinrich-Buff-Ring 26-32, D35392 Giessen, Germany.
Lodders
Nicole
N
Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, IFZ - Heinrich-Buff-Ring 26-32, D35392 Giessen, Germany.
Chou
Jui-Hsing
JH
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Shen
Fo-Ting
FT
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Frischmann
Alexa
A
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Wien, Austria.
Busse
Hans-Jürgen
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Wien, Austria.
Arun
A B
AB
Yenepoya Research Center, Yenepoya University, Nityanada Nagar, Deralakatte, Mangalore 575 018, Karnataka, India.
D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S