Generation of parthenogenetic induced pluripotent stem cells from parthenogenetic neural stem cells.
Somatic cells can achieve a pluripotent cell state in a process called pluripotential reprogramming. Multipotent stem cells can differentiate into cells of only one lineage, but pluripotent stem cells can give rise to cells of all three germ layers of an organism. In this study, we generated induced pluripotent stem (iPS) cells from bimaternal (uniparental) parthenogenetic neural stem cells (pNSCs) by transduction with either four (4F: Oct4, Klf4, Sox2, and c-Myc) or two (2F: Oct4 and Klf4) transcription factors. The resultant maternal iPS cells, which were reprogrammed directly from pNSCs, were capable of generating germ line-competent chimeras. Interestingly, analysis of global gene expression and imprinting status revealed that parthenogenetic iPS cells clustered closer to parthenogenetic ESCs than to female ESCs, with patterns that were clearly distinct from those of pNSCs.
Do
Jeong Tae
JT
Laboratory of Stem Cell and Developmental Biology, CHA Stem Cell Institute, CHA University, Seoul 135-081, Republic of Korea. dojt@cha.ac.kr
Joo
Jin Young
JY
Han
Dong Wook
DW
Araúzo-Bravo
Marcos J
MJ
Kim
Min Jung
MJ
Greber
Boris
B
Zaehres
Holm
H
Sobek-Klocke
Ingeborg
I
Chung
Hyung Min
HM
Schöler
Hans R
HR
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010312:Parthenogenesis; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism