title: [Effect of exogenous zinc addition on cell protective enzyme activities in the fruit bodies of Lentinus giganteus].
abstract: OBJECTIVE
  The effects of exogenous Zn addition on cell protective enzyme activities in the fruit bodies of Lentinus giganteus were studied.
  
  
  METHODS
  ZnSO4 was used as exogenous Zn and added into culture medium. The final Zn concentrations in culture media were 0, 10, 20, 30, 40, 50 mg/kg respectively. The activities of superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), malondialchehyche (MDA) content and soluble protein content in the fruit bodies were analyzed by spectrophotometry; catalase (CAT) was determined by potassium permanganate titration.
  
  
  RESULTS
  The content of soluble protein and SOD, POD and CAT activities in the fruit bodies of L. giganteus were significantly increased (P < 0.01), but PPO activity (P < 0.01) and MDA content (P < 0.05) was significantly decreased in the treatment of 30 mg/kg Zn concentration. The content of soluble protein and SOD, POD and CAT activities showed a decreasing trend with the increase of Zn concentration, but MDA content was significantly increased (P < 0.01 and P < 0.05).
  
  
  CONCLUSIONS
  High Zn concentration caused the increase of MDA contents and the decrease of SOD, POD and CAT activities in the fruit body of L. giganteus. It will destroy the protective enzyme system, cause the accumulation of free radicals and thus intensify membrane lipid peroxidation. Appropriate Zn concentration improved the protective enzyme activities, and lightened the harm of membrane from lipid peroxidation.
authors.affiliation: Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China. zhihe10000@163.com
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Jiang
authors.forename: Boqi
authors.initials: B
authors.lastname: Weng
authors.forename: Jingui
authors.initials: J
authors.lastname: Lei
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiangqiu
authors.initials: X
authors.lastname: Tang
authors.forename: Shuxia
authors.initials: S
authors.lastname: Xiao
mesh_terms: D002374:Catalase / Q000378:metabolism; D004156:Catechol Oxidase / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000201:enzymology / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D020074:Lentinula / Q000201:enzymology* / Q000378:metabolism; D015227:Lipid Peroxidation; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D005609:Free Radicals; D005656:Fungal Proteins; D004156:Catechol Oxidase; D002374:Catalase; D013482:Superoxide Dismutase; D015032:Zinc