Mineralized tissue formation by BMP2-transfected pulp stem cells.
Previously, in vitro differentiation of odontoblasts was shown for dental pulp stem cells (DPSCs) transfected with bone morphogenetic protein-2 (Bmp2). For this study, we hypothesized that such cells also show potential for mineralized tissue formation in vivo. DPSCs were transfected with Bmp2 and seeded onto a ceramic scaffold. These complexes were cultured in medium without dexamethasone, and thereafter placed subcutaneously in nude mice for 1, 4, and 12 weeks. Samples were evaluated by histology and real-time PCR for osteocalcin, bone sialoprotein, dentin sialophosphoprotein, and dentin matrix protein 1. Results indicated that only the transfected DPSCs showed obvious mineralized tissue generation, and 12 weeks of implantation gave the highest percentage of mineralized tissue formation (33 +/- 7.3% of implant pore area). Real-time PCR confirmed these results. In conclusion, Bmp2-transfected DPSCs effectively show mineralized tissue formation upon ectopic implantation.
Yang
X
X
Key Lab for Oral Biomedical Engineering of Ministry of Education and Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, P.R. China.
van der Kraan
P M
PM
Bian
Z
Z
Fan
M
M
Walboomers
X F
XF
Jansen
J A
JA
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000502:physiology; D002113:Calcification, Physiologic / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005822:Genetic Vectors / Q000235:genetics; D006882:Hydroxyapatites / Q000737:chemistry; D058574:Integrin-Binding Sialoprotein; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015675:Osteocalcin / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*; D040521:Subcutaneous Tissue / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D054457:Tissue Scaffolds; D014162:Transfection; D000099311:Dentin Sialophosphoprotein
D055396:Bone Morphogenetic Protein 2; D016326:Extracellular Matrix Proteins; D006882:Hydroxyapatites; D058574:Integrin-Binding Sialoprotein; D015675:Osteocalcin; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C525719:Bmp2 protein, mouse; C513308:Dmp1 protein, mouse; C546872:Ibsp protein, mouse; C074950:hydroxyapatite-beta tricalcium phosphate