Kaempferol protects HIT-T15 pancreatic beta cells from 2-deoxy-D-ribose-induced oxidative damage.
During the progression of Type 2 diabetes, glucose toxicity is likely to contribute importantly to progressive beta cell failure. Oxidative stress is an important aspect of glucose toxicity in pancreatic beta cells, and reducing sugars, such as 2-deoxy-D-ribose (dRib), produce reactive oxygen species. Furthermore, many of the biological properties of flavonoids are likely to be related to their antioxidant and free-radical scavenging abilities. Accordingly, in the present study, we investigated whether kaempferol (a flavonol) protects beta cells from dRib-induced oxidative damage. HIT-T15 cells were cultured with various concentrations of dRib for 24h. Cell survivals, amounts of reactive oxygen species (ROS) generated, apoptosis, and lipid peroxidation were measured. dRib was found to dose-dependently reduce cell survival and to markedly increase intracellular ROS levels, apoptosis, and lipid peroxidation. However, kaempferol (10 microM) suppressed dRib (20 mM) induced intracellular ROS, apoptosis, and lipid peroxidation. So, we demonstrate that kaempferol reduces dRib-mediated beta cell damage interfering with ROS metabolism and protective effects against lipid peroxidation. Our findings indicate that kaempferol protects HIT-T15 cells from dRib-induced associated oxidative damage.
Lee
Yun Jung
YJ
Department of Endocrinology and Metabolism, Kyung Hee University, School of Medicine, 1 Hoigi-Dong, Dongdaemoon-Gu, Seoul 130-702, Korea.
Suh
Kwang Sik
KS
Choi
Moon Chan
MC
Chon
Suk
S
Oh
Seungjoon
S
Woo
Jeong-Taek
JT
Kim
Sung-Woon
SW
Kim
Jin-Woo
JW
Kim
Young Seol
YS
D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D003855:Deoxyribose; D006801:Humans; D050417:Insulin-Secreting Cells / Q000187:drug effects*; D044949:Kaempferols / Q000494:pharmacology*; D015227:Lipid Peroxidation; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D044949:Kaempferols; D017382:Reactive Oxygen Species; D003855:Deoxyribose; C006552:kaempferol