Screening and characterization of a phosphopentomutase useful for enzymatic production of 2'-deoxyribonucleoside.
Bacillus sphaericus AKU 229 was found to produce an acetaldehyde-tolerant and phosphorylated compound-tolerant phosphopentomutase useful for enzymatic 2'-deoxyribonucleoside production. The gene encoding the phosphopentomutase was cloned and expressed in Escherichia coli. The E. coli expressing B. sphaericus phosphopentomutase was an excellent catalyst as to production of 2'-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds such as fructose 1,6-diphosphate, and d-glyceraldehyde 3-phosphate, which are derived from glucose through glycolysis with yeast cells, and exist abundantly in the practical reaction mixture for enzymatic 2'-deoxyribonucleoside production.
Horinouchi
Nobuyuki
N
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Kawano
Takako
T
Sakai
Takafumi
T
Matsumoto
Seiichiro
S
Sasaki
Mie
M
Mikami
Yoichi
Y
Ogawa
Jun
J
Shimizu
Sakayu
S
D000079:Acetaldehyde / Q000494:pharmacology; D000595:Amino Acid Sequence; D001407:Bacillus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D055162:Biocatalysis / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D003853:Deoxyribonucleosides / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D010770:Phosphotransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014170:Transformation, Genetic / Q000187:drug effects
D003853:Deoxyribonucleosides; D010770:Phosphotransferases; C022375:phosphopentomutase; D000079:Acetaldehyde