title: [Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in somatic cytochrome C gene].
abstract: Mouse embryonic fibroblasts (MEF) with point mutation in somatic cytochrome C gene were generated and characterized. It was shown that substitution of lysine for tryptophan in position 72 (K72W) decreased the proapoptotic functions of cytochrome C in response to staurosporin treatment without disrupting its respiratory functions. The presence of this mutation did not affect the pattern of cytochrome C gene expression or its localization inside the cell. These cell cultures therefore represent an interesting model for study apoptotic signaling and physiological functions of cytochrome C.
authors.forename: I A
authors.initials: IA
authors.lastname: Mufazalov
authors.forename: D N
authors.initials: DN
authors.lastname: Pen'kov
authors.forename: B V
authors.initials: BV
authors.lastname: Cherniak
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Pletiushkina
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Vysokikh
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
authors.forename: D A
authors.initials: DA
authors.lastname: Dolgikh
authors.forename: A A
authors.initials: AA
authors.lastname: Kruglov
authors.forename: D V
authors.initials: DV
authors.lastname: Kuprash
authors.forename: V P
authors.initials: VP
authors.lastname: Skulachev
authors.forename: S A
authors.initials: SA
authors.lastname: Nedospasov
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002460:Cell Line; D045304:Cytochromes c / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D008954:Models, Biological; D020125:Mutation, Missense; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D045304:Cytochromes c; D019311:Staurosporine