Synthesis and characterization of a novel molecularly imprinted polymer for simultaneous extraction and determination of water-soluble and fat-soluble synthetic colorants in chilli products by solid phase extraction and high performance liquid chromatography.
A sorbent was synthesized and investigated for molecularly imprinted solid phase extraction (MISPE). Molecularly imprinted polymers (MIP) were synthesized via precipitation polymerization procedure, where 4-vinyl pyridine (4-VP) was used as functional monomer and ethylene glycol dimethacrylate (EDMA) as cross-linking agent. The imprinting effect of the MISPE was evaluated by elution experiments. The resulting MISPE showed high extraction selectivity to water-soluble and fat-soluble synthetic colorants. The determination of multi-residue for three kinds of water-soluble and six kinds of fat-soluble synthetic colorants in chilli products was also investigated by HPLC coupled with MISPE. The mean recoveries calculated by solvent calibration curve for water-soluble and fat-soluble synthetic colorants were from 72.1% to 95.6% for chilli spice and 72.1% to 92.3% for chilli powder. The decision limit (CCalpha) and the detection capability (CCbeta) obtained for water-soluble and fat-soluble synthetic colorants were in the range of 1.2-1.6 and 1.9-2.4 microg kg(-1) in chilli spice and chilli powder. The resulting MISPE was successfully used off-line for the determination of nine kinds of synthetic colorants in chilli products.
Long
Chaoyang
C
Center for Disease Prevention and Control of Guangdong Province, Guangzhou 510300, China. chaoyang_long@163.com
Mai
Zhibin
Z
Yang
Yingfen
Y
Zhu
Binghui
B
Xu
Xiumin
X
Lu
Lin
L
Zou
Xiaoyong
X
D001391:Azo Compounds / Q000032:analysis*; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004396:Coloring Agents / Q000032:analysis* / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D005561:Formates / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D009284:Naphthols / Q000032:analysis*; D011145:Polyvinyls / Q000138:chemical synthesis / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility
D001391:Azo Compounds; D004396:Coloring Agents; D005561:Formates; D008689:Methacrylates; D009284:Naphthols; D011145:Polyvinyls; D011725:Pyridines; C030544:formic acid; C019535:poly(4-vinylpyridine); C004919:ethylene dimethacrylate; C029351:4-vinylpyridine; D000432:Methanol