title: [Diversity of soil archaea in Tibetan Mila Mountains].
abstract: OBJECTIVE
  In order to study the diversity of archaea and ammonia-oxidizing archaea (AOA) of the alp prairie soil in Mila Mountain of Tibet.
  
  
  METHODS
  Total microbial DNA was directly extracted from the alp prairie of Mila Mountain. The clone library of 16S rRNA genes and amoA genes were amplified by PCR with universal primer sets. The sequences of archaea and AOA were defined into operational taxonomic units (OTUs) according to the 97% similarity threshold for OTU assignment was performed using the software program DOTUR.
  
  
  RESULTS
  Phylogenetic analysis revealed archaea in the soil of Mila Mountain including the Crenarchaeota (71.7%) and unclassified-Archaea (28.3%) phyla. All the Crenarchaeota belong to the Thermoprotei. Phylogenetic analysis revealed AOA in the alp prairie soil of Mila Mountain belonged to the kingdom Crenarchaeota. Archaea and AOA species composition from Mila Mountain included 64 OTUs and 75 OTUs.
  
  
  CONCLUSIONS
  These findings show prolific archaeal diversity in the alp prairie soil of Mila Mountain, where they may be actively involved in nitrification.
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. xiaowei031@163.com
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Meng
authors.forename: Zhenchuan
authors.initials: Z
authors.lastname: Mao
authors.forename: Guohua
authors.initials: G
authors.lastname: Chen
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Yang
authors.forename: Bingyan
authors.initials: B
authors.lastname: Xie
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D019843:Archaeal Proteins / Q000235:genetics; D044822:Biodiversity; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D018609:Tibet
chemical_list: D019843:Archaeal Proteins; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000641:Ammonia