title: Molecular epidemiology of rotavirus diarrhea among children aged <5 years in nepal: predominance of emergent G12 strains during 2 years.
abstract: A 2-year surveillance was performed in Kathmandu, Nepal, by collection of stool specimens from 1139 children aged <5 years who were hospitalized for acute diarrhea from November 2005 through October 2007. Of the 1139 samples, 379 (33%) had rotavirus strains identified by enzyme-linked immunosorbent assay; the most prevalent G type was G12, accounting for 50% of typed strains in 2005-2006 and 29% in 2006-2007, followed by G1 (26%) in 2005-2006 and by G9 (28%) and G2 (20%) in 2006-2007. The most prevalent P type was P[8], accounting for 47% of strains in 2005-2006 and 35% in 2006-2007, followed by P[6] (37% in 2005-2006 and 33% in 2006-2007) and P[4] (10% in 2005-2006 and 24% in 2006-2007). Of combined genotypes, G12P[6] was the most prevalent, accounting for 34% of strains in 2005-2006 and 24% in 2006-2007, followed by G1P[8] (23%) in 2005-2006 and G2P[4] (20%) in 2006-2007. An unusually high detection of G12 strains underscores the importance of continued surveillance of rotavirus strains.
authors.affiliation: Department of Clinical Microbiology and Health Research Laboratory, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal.
authors.forename: Jeevan Bahadur
authors.initials: JB
authors.lastname: Sherchand
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakagomi
authors.forename: Winifred
authors.initials: W
authors.lastname: Dove
authors.forename: Toyoko
authors.initials: T
authors.lastname: Nakagomi
authors.forename: Michiyo
authors.initials: M
authors.lastname: Yokoo
authors.forename: Basu Dev
authors.initials: BD
authors.lastname: Pandey
authors.forename: Luis E
authors.initials: LE
authors.lastname: Cuevas
authors.forename: C Anthony
authors.initials: CA
authors.lastname: Hart
authors.forename: Nigel A
authors.initials: NA
authors.lastname: Cunliffe
mesh_terms: D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009390:Nepal / Q000453:epidemiology; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D013997:Time Factors