Begomovirus coat protein interacts with a small heat-shock protein of its transmission vector (Bemisia tabaci).
Tomato yellow leaf curl Sardinia virus (TYLCSV) is transmitted from plant to plant by the whitefly Bemisia tabaci in a persistent-circulative manner. The coat protein (CP) plays an important role in this transmission cycle. In this study, the CP was used to screen a Bemisia tabaci cDNA library using the yeast two-hybrid system, in a search for interacting partners. A member of the small heat-shock protein family (termed BtHSP16) was identified and its interaction with the CP was verified by an in vitro pull-down assay. The binding domain was located at the variable N-terminal part of the CP, while full-length BtHSP16 is required for the interaction. The putative role for this interaction in the transmission cycle by the whitefly is discussed.
Ohnesorge
S
S
Unidad de Genética, Departamento de Biología Celular, Genética y Fisiología, Universidad de Málaga, Málaga, Spain.
Bejarano
E R
ER
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D053839:Begomovirus / Q000378:metabolism*; D036022:Capsid Proteins / Q000378:metabolism*; D015723:Gene Library; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006430:Hemiptera / Q000821:virology*; D015151:Immunoblotting; D007303:Insect Vectors / Q000821:virology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010935:Plant Diseases / Q000821:virology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D020798:Two-Hybrid System Techniques
D036022:Capsid Proteins; D006360:Heat-Shock Proteins; D009841:Oligonucleotides