In vitro adsorption of tear proteins to hydroxyethyl methacrylate-based contact lens materials.
OBJECTIVE
Investigations of polymer interactions in single protein solutions is a necessary step in the elucidation of in vivo early binding events during protein deposition on hydroxyethyl methacrylate-based contact lens materials. Quantity and tenacity of binding of significant tear components to groups I and IV contact lenses was assessed. Competitive binding by these components was also examined.


METHODS
Adsorption on FDA groups I and IV hydrogel lenses was monitored using I-labeled protein. Lenses were incubated in increasing concentrations of radiolabeled single species proteins in solution. For competition experiments, concentration of each radiolabeled protein was held constant and the adsorption/sorption challenged with increasing concentrations of nonlabeled proteins. Lenses were soaked in phosphate-buffered saline to determine desorption.


RESULTS
Group IV lenses bound large amounts of lysozyme, whereas group I lenses bound highest amounts of albumin. Albumin binding to both lens types was relatively strong and could not be competed from binding by other proteins lysozyme, lactoferrin, and mucin. Mucin at high concentrations tended to positively cooperate with the binding of lactoferrin and albumin to all lenses.


CONCLUSIONS
Binding of proteins to hydroxyethyl methacrylate-based hydrogel lens surfaces is affected by charge and polymer components, and perhaps manufacturing processes. Albumin binds strongly to lens surfaces, and this may play an adverse role during contact lens wear.
Carney
Fiona P
FP
Vision Cooperative Research Centre, Sydney, New South Wales, Australia.
Morris
Carol A
CA
Milthorpe
Bruce
B
Flanagan
Judith L
JL
Willcox
Mark D P
MD
D000327:Adsorption; D000418:Albumins / Q000378:metabolism; D001667:Binding, Competitive; D003263:Contact Lenses, Hydrophilic / Q000145:classification; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007781:Lactoferrin / Q000378:metabolism; D008689:Methacrylates; D009077:Mucins / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009994:Osmolar Concentration; D013696:Temperature; D013997:Time Factors
D000418:Albumins; D005136:Eye Proteins; D008689:Methacrylates; D009077:Mucins; C042434:tear proteins; C005044:hydroxyethyl methacrylate; D009113:Muramidase; D007781:Lactoferrin