title: In situ single cell monitoring by isocyanide-functionalized Ag and Au nanoprobe-based Raman spectroscopy.
abstract: The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanide functionalized silver and gold nanoparticles by surface enhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at approximately 2,100 cm-1 in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and 3,600 cm-1. The SERS marker band at approximately 2,100 cm-1 could be used to judge the location of the isocyanide-functionalized nanoparticles inside the cell without much spectral interference from other cellular constituents. Our results demonstrate that isocyanide-modified silver or gold nanoparticle-based SERS may have high potential for monitoring and imaging the biological processes at the single cell level.
authors.affiliation: Department of Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors.forename: So Yeong
authors.initials: SY
authors.lastname: Lee
authors.forename: Soo Hwa
authors.initials: SH
authors.lastname: Jang
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Young Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Keun Chang
authors.initials: KC
authors.lastname: Cho
authors.forename: Pan Dong
authors.initials: PD
authors.lastname: Ryu
authors.forename: Myoung-Seon
authors.initials: MS
authors.lastname: Gong
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Joo
mesh_terms: D000818:Animals; D019556:COS Cells / Q000648:ultrastructure; D002477:Cells / Q000648:ultrastructure*; D002522:Chlorocebus aethiops; D003486:Cyanides; D006046:Gold; D007202:Indicators and Reagents; D017953:Isocyanates; D008322:Mammals / Q000502:physiology; D008854:Microscopy, Electron; D053758:Nanoparticles; D012542:Scattering, Radiation; D012834:Silver; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: C476736:2-methylpentane-diisocyanate; D003486:Cyanides; D007202:Indicators and Reagents; D017953:Isocyanates; D012834:Silver; D006046:Gold