title: [Metabolic characteristics of GS-nS0 myeloma cells producing anti-CD25 monoclonal antibody in serum-free culture].
abstract: As an immunodepressant, anti-CD25 monoclonal antibody has a huge market with wide prospect and economic value. We developed a low protein serum-free medium for large-scale GS-NS0 myeloma cell culture and anti-CD25 monoclonal antibody production. Further study focused on the characteristics of GS-NSO cell growth, glucose and amino acid metabolism, and antibody production. In the serum-free medium, the maximal viable cell density and antibody concentration reached above 3x10(6) cells/mL and 300 mg/L in batch culture. Compared with the commercial serum-free medium (Excell 620 + 0.2% Primatone), the maximal viable cell density doubled and the maximal antibody concentration increased 46%. Results also showed the specific growth rate decreased when the glucose concentration was lower than 6 mmol/L. And the production of lactate increased when glucose concentration was excessively high (> 30 mmol/L). These results were important to provide technique and theory basis for developing optimized GS-NS0 cell culture and anti-CD25 monoclonal antibody production processes.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Wensong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free; D053645:Interleukin-2 Receptor alpha Subunit; D011994:Recombinant Proteins