Isolation and identification of a Candida digboiensis strain from an extreme acid mine drainage of the Lignite Mine, Gujarat.
An extremely acidic mine drainage (AMD) water sample was collected in 1998 and 2008 from Panandhro lignite mine, Gujarat, India. The yeast isolated from this sample was identified using mini API identification system, as a member of genus Candida. The major cellular fatty acids detected by FAME from the isolate are C(16:0) and C(18:2) (cis 9,12)/C(18:0alpha) as 25.23 and 19.5%, respectively. The isolate was identified as Candida digboiensis by 18S rRNA gene sequence analysis and designated as Candida digboiensis SRDyeast1. Phylogenetic analysis using D1/D2 variable domains showed that the closest relative of this strain is Candida blankii with 3% divergence. This organism has been reported for the first time from the lignite mine AMD sample, and for cellular fatty acid analysis. This yeast is able to survive in the AMD sample preserved at 10-42 degrees C temperature since last 10 years along with iron oxidizing microorganisms. It can grow in the presence of 40% glucose, 10% NaCl and in the pH range of 1 to 10. The isolate is capable of producing enzymes like protease and lipase. This isolate differs from the type strain Candida digboiensis in as many as six physiological and metabolic characteristics.
Patel
Mitesh J
MJ
Department of Microbiology, School of Sciences, Gujarat University, Ahmedabad 380 009, India.
Tipre
Devayani R
DR
Dave
Shailesh R
SR
D000143:Acids; D002175:Candida / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D004271:DNA, Fungal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007194:India; D008826:Microbial Sensitivity Tests; D008906:Mining; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014874:Water Pollutants, Chemical
D000143:Acids; D003470:Culture Media; D004271:DNA, Fungal; D005227:Fatty Acids; D012337:RNA, Ribosomal, 18S; D014874:Water Pollutants, Chemical