title: Levels of DNA adducts in the blood and follicular fluid of women undergoing in vitro fertilization treatment and its correlation with the pregnancy outcome.
abstract: This study is designed to investigate the impact of DNA damage on pregnancy and fertilization rate outcome in a sub-sample of women undergoing IVF treatment. Blood and follicular fluid samples (n = 60) were analyzed for DNA adducts. While no BPDE-DNA adducts were detected, other unknown lipophilic adducts were seen in blood and follicular fluid. Women who failed to achieve pregnancy had higher DNA adducts in follicular fluid than those who succeeded (p < 0.05). Follicular fluid cotinine levels were associated with DNA adduct levels in blood and follicular fluid (p < 0.05). Evaluation of DNA damage resulting from oxidative stress could have a role in predicting IVF success rate.
authors.affiliation: Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Saudi Arabia. iman@kfshrc.edu.sa
authors.forename: Iman
authors.initials: I
authors.lastname: Al-Saleh
authors.forename: Inaam
authors.initials: I
authors.lastname: El-Doush
authors.forename: Jamal
authors.initials: J
authors.lastname: Arif
authors.forename: Serdar
authors.initials: S
authors.lastname: Coskun
authors.forename: Kamal
authors.initials: K
authors.lastname: Jaroudi
authors.forename: Abdulaziz
authors.initials: A
authors.lastname: Al-Shahrani
authors.forename: Gamal
authors.initials: G
authors.lastname: El-Din Mohamed
mesh_terms: D000328:Adult; D003367:Cotinine / Q000032:analysis; D018736:DNA Adducts / Q000032:analysis / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry; D006801:Humans; D019216:Metals, Heavy / Q000097:blood; D018384:Oxidative Stress; D011247:Pregnancy; D011256:Pregnancy Outcome
chemical_list: D018736:DNA Adducts; D019216:Metals, Heavy; D003367:Cotinine