Bacillus subtilis spreads by surfing on waves of surfactant.
The bacterium Bacillus subtilis produces the molecule surfactin, which is known to enhance the spreading of multicellular colonies on nutrient substrates by lowering the surface tension of the surrounding fluid, and to aid in the formation of aerial structures. Here we present experiments and a mathematical model that demonstrate how the differential accumulation rates induced by the geometry of the bacterial film give rise to surfactant waves. The spreading flux increases with increasing biofilm viscosity. Community associations are known to protect bacterial populations from environmental challenges such as predation, heat, or chemical stresses, and enable digestion of a broader range of nutritive sources. This study provides evidence of enhanced dispersal through cooperative motility, and points to nonintuitive methods for controlling the spread of biofilms.
Angelini
Thomas E
TE
School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA. angelini@seas.harvard.edu
Roper
Marcus
M
Kolter
Roberto
R
Weitz
David A
DA
Brenner
Michael P
MP
D001412:Bacillus subtilis / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D001422:Bacterial Adhesion; D018441:Biofilms; D055666:Lipopeptides / Q000096:biosynthesis*; D008954:Models, Biological; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D013501:Surface-Active Agents / Q000378:metabolism*; D014783:Viscosity
D055666:Lipopeptides; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C009365:surfactin peptide