Identification and genomic location of a reniform nematode (Rotylenchulus reniformis) resistance locus (Ren ari) introgressed from Gossypium aridum into upland cotton (G. hirsutum).
In this association mapping study, a tri-species hybrid, [Gossypium arboreum x (G. hirsutum x G. aridum)(2)], was crossed with MD51ne (G. hirsutum) and progeny from the cross were used to identify and map SSR markers associated with reniform nematode (Rotylenchulus reniformis) resistance. Seventy-six progeny (the 50 most resistant and 26 most susceptible) plants were genotyped with 104 markers. Twenty-five markers were associated with a resistance locus that we designated Ren(ari) and two markers, BNL3279_132 and BNL2662_090, mapped within 1 cM of Ren(ari). Because the SSR fragments associated with resistance were found in G. aridum and the bridging line G 371, G. aridum is the likely source of this resistance. The resistance is simply inherited, possibly controlled by a single dominant gene. The markers identified in this project are a valuable resource to breeders and geneticists in the quest to produce cotton cultivars with a high level of resistance to reniform nematode.
Romano
Gabriela Beatriz
GB
USDA/ARS, 141 Experiment Station Rd, Stoneville, MS 38776, USA. gabriela.romano@ars.usda.gov
Sacks
Erik J
EJ
Stetina
Salliana R
SR
Robinson
A Forest
AF
Fang
David D
DD
Gutierrez
Osman A
OA
Scheffler
Jodi A
JA
D000818:Animals; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018556:Crops, Agricultural / Q000469:parasitology; D003433:Crosses, Genetic; D005819:Genetic Markers; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics / Q000469:parasitology; D007113:Immunity, Innate / Q000235:genetics*; D010935:Plant Diseases / Q000469:parasitology*; D014431:Tylenchoidea / Q000472:pathogenicity*
D005819:Genetic Markers