Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection.
A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.
Cheng
Jilin
J
Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.
Zhou
Xin
X
Chou
Tsui-Fen
TF
Ghosh
Brahma
B
Liu
Baoling
B
Wagner
Carston R
CR
D000577:Amides / Q000737:chemistry*; D000596:Amino Acids / Q000378:metabolism; D019380:Anti-HIV Agents / Q000378:metabolism; D017123:Bacteriophage T7 / Q000378:metabolism*; D003561:Cytarabine / Q000494:pharmacology; D016503:Drug Delivery Systems; D015195:Drug Design; D006801:Humans; D019151:Peptide Library; D010756:Phosphoric Acids / Q000737:chemistry*; D014779:Virus Replication; D015215:Zidovudine / Q000378:metabolism*
D000577:Amides; D000596:Amino Acids; D019380:Anti-HIV Agents; D019151:Peptide Library; D010756:Phosphoric Acids; D003561:Cytarabine; D015215:Zidovudine; C011067:phosphoramidic acid