Comparison of two chromogenic media for selective isolation of vancomycin-resistant enterococci from stool specimens.
Two chromogenic media (Chromagar VRE and chromID VRE [C-ID]) performed equally well in the direct detection of vancomycin-resistant enterococci (VRE) in stool specimens after an overnight enrichment step and a 48-h incubation period, with a sensitivity of 98.2% (56/57) for both and specificities of 96.5% (195/202) and 97.5% (197/202), respectively. However, assigning discriminatory colony color was sometimes difficult, especially on C-ID. In order to facilitate simple species identification, biochemical key reactions were implemented.
Peltroche-Llacsahuanga
Heidrun
H
Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany.
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Janetta
J
Weber-Heynemann
Josefine
J
Lütticken
Rudolf
R
Haase
Gerhard
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002863:Chromogenic Compounds; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D016983:Enterococcus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity; D020713:Vancomycin Resistance / Q000235:genetics
D000900:Anti-Bacterial Agents; D002863:Chromogenic Compounds; D003470:Culture Media