Other than IPG-DALT: 2-DE variants.
Special problems require special solutions. That is why, despite the trend towards standardization of the procedures in proteomic investigations, a number of alternative protocols, based on 2-DE, have been devised with time and are applied to the resolution of proteins on which the performance of IPG-DALT is poor. The difficult-to-handle samples include high pI, high molecular size and high hydrophobicity proteins. The protocol variants entail changes in the gel media and/or the use of unusual additives (often surfactants or solvents). But also: Specific questions require specific answers. When the aim is not the resolution of individual polypeptide chains in a fully unfolded state but the analysis of higher-order structures (proteins assembled from subunits, complexes assembled from interacting proteins) the standard conditions under which IPG-DALT is performed turn inadequate. Electrophoresis is then performed either in first dimension or in both first dimension and second dimension under non-denaturing (native) and/or non-reducing conditions.
Miller
Ingrid
I
Medizinische Chemie, Department für Biomedizinische Wissenschaften, Veterinärmedizinische Universität Wien, Wien, Austria.
Eberini
Ivano
I
Gianazza
Elisabetta
E
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D011489:Protein Denaturation; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D040901:Proteomics / Q000379:methods*; D013048:Specimen Handling
D021122:Protein Subunits