title: Structure of yellow fever virus envelope protein domain III.
abstract: The structure of recombinant domain III of the envelope protein (rED3) of yellow fever virus (YFV), containing the major neutralization site, was determined using NMR spectroscopy. The amino acid sequence and structure of the YFV-rED3 shows differences from ED3s of other mosquito-borne flaviviruses; in particular, the partially surface-exposed BC loop where methionine-304 and valine-324 were identified as being critical for the structure of the loop. Variations in the structure and surface chemistry of ED3 between flaviviruses affect neutralization sites and may affect host cell receptor interactions and play a role in the observed variations in viral pathogenesis and tissue tropism.
authors.affiliation: Departments of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555-1157, USA.
authors.forename: David E
authors.initials: DE
authors.lastname: Volk
authors.forename: Fiona J
authors.initials: FJ
authors.lastname: May
authors.forename: Sai H A
authors.initials: SH
authors.lastname: Gandham
authors.forename: Anjenique
authors.initials: A
authors.lastname: Anderson
authors.forename: Jana J
authors.initials: JJ
authors.lastname: Von Lindern
authors.forename: David W C
authors.initials: DW
authors.lastname: Beasley
authors.forename: Alan D T
authors.initials: AD
authors.lastname: Barrett
authors.forename: David G
authors.initials: DG
authors.lastname: Gorenstein
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics; D015005:Yellow fever virus / Q000737:chemistry* / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins