Acute microinstillation inhalation exposure to sarin induces changes in respiratory dynamics and functions in guinea pigs.
This study investigates the toxic effects of sarin on respiratory dynamics following microinstillation inhalation exposure in guinea pigs. Animals are exposed to sarin for 4 minutes, and respiratory functions are monitored at 4 hours and 24 hours by whole-body barometric plethysmography. Data show significant changes in respiratory dynamics and function following sarin exposure. An increase in respiratory frequency is observed at 4 hours post exposure compared with saline controls. Tidal volume and minute volume are also increased in sarin-exposed animals 4 hours after exposure. Peak inspiratory flow increases, whereas peak expiratory flow increases at 4 hours and is erratic following sarin exposure. Animals exposed to sarin show a significant decrease in expiratory time and inspiratory time. End-inspiratory pause is unchanged whereas end-expiratory pause is slightly decreased 24 hours after sarin exposure. These results indicate that inhalation exposure to sarin alters respiratory dynamics and function at 4 hours, with return to normal levels at 24 hours post exposure.
Conti
Michele L
ML
United States Army Medical Research Institute of Chemical Defense, Edgewood, MD, USA.
Che
Magnus M
MM
Boylan
Megan
M
Sciuto
Alfred M
AM
Gordon
Richard K
RK
Nambiar
Madhusoodana P
MP
D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002619:Chemical Warfare Agents / Q000633:toxicity*; D006168:Guinea Pigs; D019570:Inhalation Exposure / Q000009:adverse effects*; D007928:Lethal Dose 50; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010100:Oxygen / Q000097:blood; D010993:Plethysmography, Whole Body; D011654:Pulmonary Edema / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D012143:Respiratory Physiological Phenomena / Q000187:drug effects*; D012524:Sarin / Q000633:toxicity*
D002619:Chemical Warfare Agents; D012524:Sarin; D000110:Acetylcholinesterase; D010100:Oxygen