Cardiac matrix remodeling following intracoronary cell transplantation in dilated cardiomyopathic rabbits.
Cellular cardiomyoplasty has been proposed as a promising therapeutic strategy for chronic heart failure. Previous studies focused on structural changes in cardiomyocytes to explain the potential benefits for contractile function. However, limited information is available about the cardiac matrix remodeling following cell transplantation in dilated cardiomyopathy (DCM). Here, we established a new animal model of intracoronary bone marrow mononuclear cells (BMMNCs) transplantation to explore extracellular matrix remodeling in adriamycin-induced cardiomyopathic rabbits. In vivo studies demonstrated that BMMNCs transplantation can dramatically delay the progress of collagen metabolism and decrease myocardial collagen volume fraction. The beneficial effects were mediated by attenuating stress-generated over-expression of matrix metalloproteinases (MMPs) in ventricular remodeling. Improved cardiac function may be contributed in part by stem-associated inhibition of extracellular matrix remodeling.
Jin
Bo
B
Department of Cardiology, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China.
Luo
Xin-Ping
XP
Ni
Huan-Chun
HC
Li
Yong
Y
Shi
Hai-Ming
HM
D000818:Animals; D015153:Blotting, Western; D016026:Bone Marrow Transplantation; D002311:Cardiomyopathy, Dilated / Q000097:blood / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D017023:Coronary Angiography; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D006439:Hemodynamics; D009206:Myocardium / Q000473:pathology* / Q000648:ultrastructure; D020097:Natriuretic Peptide, Brain / Q000097:blood; D011817:Rabbits; D013194:Staining and Labeling; D016019:Survival Analysis
D020097:Natriuretic Peptide, Brain; D003094:Collagen