title: Concentrated collagen hydrogels as dermal substitutes.
abstract: Collagen hydrogels first appeared promising for skin repair. Unfortunately, their extensive contraction and their poor mechanical properties constituted major disadvantages toward their utilization as permanent graft. The present study has investigated a way to correct these drawbacks by increasing the collagen concentration in controlled conditions. Concentrated collagen hydrogels (CCH) at 1.5, 3 and 5mg/ml were obtained. The effect of raised collagen concentration on contraction, cell growth and remodeling activities was evaluated for 21 days in culture. Subsequently, in vivo integration of CCH and normal collagen hydrogels (NCH) was assessed. Compared to NCH, CCH contraction was delayed and smaller. At day 21, surface area of CCH at 3mg/ml was 18 times more important than that of NCH. Whatever the initial fibroblast density, CCH favored cell growth that reached about 10 times the initial cell number at day 21; cell proliferation was inhibited in NCH. Gelatinase A activities appeared lower in CCH than within NCH. In vivo studies in rats revealed a complete hydrolysis of NCH 15 days after implantation. In contrast, CCH at 3mg/ml was still present after 30 days. Moreover, CCH showed cell colonization, neovascularization and no severe inflammatory response. Our results demonstrate that concentrated collagen hydrogels can be considered as new candidates for dermal substitution because they are is easy to handle, do not contract drastically, favor cell growth, and can be quickly integrated in vivo.
authors.affiliation: CMCP-CNRS UMR7574, EPHE, University Pierre and Marie Curie, Paris, France. chelary@snv.jussieu.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Helary
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bataille
authors.forename: Aicha
authors.initials: A
authors.lastname: Abed
authors.forename: Corinne
authors.initials: C
authors.lastname: Illoul
authors.forename: Annie
authors.initials: A
authors.lastname: Anglo
authors.forename: Liliane
authors.initials: L
authors.lastname: Louedec
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.forename: Anne
authors.initials: A
authors.lastname: Meddahi-Pellé
authors.forename: Marie Madeleine
authors.initials: MM
authors.lastname: Giraud-Guille
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D033081:Shear Strength; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D003094:Collagen; D020778:Matrix Metalloproteinase 2