title: Leukocyte ultrastructure, hematological and serum biochemical profiles of ostriches (Struthio camelus).
abstract: In the last decade ostrich farms spread throughout the world as an alternative source of investment. Although previous studies have reported hematology and biochemical values for ostriches from several regions of the world, little information is available regarding leukocyte morphology. This study reports the morphology and ultrastructure of ostrich leukocytes and hematology and biochemical values from birds raised in Brazil. Heterophils presented a lobulated nucleus, and fusiform, and acidophilic and peroxidase-negative granules. Ultrastructurally, 2 kinds of cytoplasmic granules were observed: one was large and fusiform and the other smaller with heterogeneous morphology and electrondensity; granules were peroxidase-negative. Eosinophils had a kidney-shaped eccentrically placed nucleus that was rarely lobulated and eosinophilic, round, and peroxidase-positive granules. At the ultrastructure level, 2 main kinds of granules with the same size and form but different electron density were seen; granules were peroxidase-positive. Lymphocytes and thrombocytes had the same characteristics of other avian species; monocytes presented morphological heterogeneity. Hematological and serum biochemical profiles had no sex influence and were established for ostriches raised in southeastern Brazil. These parameters will help the diagnosis of specific ostrich pathologies and serve as basic knowledge for studies in immunology and comparative avian pathology.
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Brazil.
authors.forename: S F
authors.initials: SF
authors.lastname: Bonadiman
authors.forename: G C
authors.initials: GC
authors.lastname: Stratievsky
authors.forename: J A
authors.initials: JA
authors.lastname: Machado
authors.forename: A P
authors.initials: AP
authors.lastname: Albernaz
authors.forename: G R
authors.initials: GR
authors.lastname: Rabelo
authors.forename: R A
authors.initials: RA
authors.lastname: Damatta
mesh_terms: D000818:Animals; D007962:Leukocytes / Q000648:ultrastructure*; D020310:Struthioniformes / Q000097:blood*