title: Is there a link between the lipopolysaccharide of Helicobacter pylori gastric MALT lymphoma associated strains and lymphoma pathogenesis?
abstract: The aim of this study was to investigate the Lewis antigen expression in Helicobacter pylori gastric MALT lymphoma associated strains in comparison to chronic gastritis only strains. Forty MALT strains (19 cagPAI (-) and 21 cagPAI (+)) and 39 cagPAI frequency-matched gastritis strains (17 cagPAI (-) and 22 cagPAI (+)) were included in this study. The lipopolyssacharide for each strain was extracted using a hot phenol method and the expression of Le(x) and Le(y) were investigated using Western Blot. The data were analyzed according to the strains' cagPAI status and vacA genotype. Le(x) was identified in 21 (52.5%) MALT strains and 29 (74.3%) gastritis strains. Le(y) was identified in 30 (75%) MALT strains and 31 (79.5%) gastritis strains. There was an association between cagPAI positivity and Le(x) expression among MALT strains (p<0.0001), but not in gastritis strains (p = 0.64). Among cagPAI (-) strains, isolates expressing solely Le(y) were associated with MALT with an odds ratio of 64.2 (95% CI 4.9-841.0) when compared to strains expressing both Le(x) and Le(y). vacA genotypes did not modify the association between Lewis antigen expression and disease status. In conclusion, cagPAI (-) MALT strains have a particular Lewis antigen profile which could represent an adaptive mechanism to the host response or participate in MALT lymphomagenesis.
authors.affiliation: INSERM, U853, Bordeaux, France. philippe.lehours@labhel.u-bordeaux2.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Lehours
authors.forename: Zongli
authors.initials: Z
authors.lastname: Zheng
authors.forename: Anna
authors.initials: A
authors.lastname: Skoglund
authors.forename: Francis
authors.initials: F
authors.lastname: Mégraud
authors.forename: Lars
authors.initials: L
authors.lastname: Engstrand
mesh_terms: D000328:Adult; D001426:Bacterial Proteins; D049109:Cell Proliferation; D003433:Crosses, Genetic; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000378:metabolism; D016256:Lewis X Antigen / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000382:microbiology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; C094162:Lewis Y antigen; D008070:Lipopolysaccharides; C074689:VacA protein, Helicobacter pylori