title: Highly tumorigenic lung cancer CD133+ cells display stem-like features and are spared by cisplatin treatment.
abstract: The identification of lung tumor-initiating cells and associated markers may be useful for optimization of therapeutic approaches and for predictive and prognostic information in lung cancer patients. CD133, a surface glycoprotein linked to organ-specific stem cells, was described as a marker of cancer-initiating cells in different tumor types. Here, we report that a CD133+, epithelial-specific antigen-positive (CD133+ESA+) population is increased in primary nonsmall cell lung cancer (NSCLC) compared with normal lung tissue and has higher tumorigenic potential in SCID mice and expression of genes involved in stemness, adhesion, motility, and drug efflux than the CD133(-) counterpart. Cisplatin treatment of lung cancer cells in vitro resulted in enrichment of CD133+ fraction both after acute cytotoxic exposure and in cells with stable cisplatin-resistant phenotype. Subpopulations of CD133+ABCG2+ and CD133+CXCR4+ cells were spared by in vivo cisplatin treatment of lung tumor xenografts established from primary tumors. A tendency toward shorter progression-free survival was observed in CD133+ NSCLC patients treated with platinum-containing regimens. Our results indicate that chemoresistant populations with highly tumorigenic and stem-like features are present in lung tumors. The molecular features of these cells may provide the rationale for more specific therapeutic targeting and the definition of predictive factors in clinical management of this lethal disease.
authors.affiliation: Molecular Cytogenetics Unit, Department of Experimental Oncology, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico, Istituto Nazionale dei Tumori, 20133 Milan, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Bertolini
authors.forename: Luca
authors.initials: L
authors.lastname: Roz
authors.forename: Paola
authors.initials: P
authors.lastname: Perego
authors.forename: Monica
authors.initials: M
authors.lastname: Tortoreto
authors.forename: Enrico
authors.initials: E
authors.lastname: Fontanella
authors.forename: Laura
authors.initials: L
authors.lastname: Gatti
authors.forename: Graziella
authors.initials: G
authors.lastname: Pratesi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Fabbri
authors.forename: Francesca
authors.initials: F
authors.lastname: Andriani
authors.forename: Stella
authors.initials: S
authors.lastname: Tinelli
authors.forename: Elena
authors.initials: E
authors.lastname: Roz
authors.forename: Roberto
authors.initials: R
authors.lastname: Caserini
authors.forename: Salvatore
authors.initials: S
authors.lastname: Lo Vullo
authors.forename: Tiziana
authors.initials: T
authors.lastname: Camerini
authors.forename: Luigi
authors.initials: L
authors.lastname: Mariani
authors.forename: Domenico
authors.initials: D
authors.lastname: Delia
authors.forename: Elisa
authors.initials: E
authors.lastname: Calabrò
authors.forename: Ugo
authors.initials: U
authors.lastname: Pastorino
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sozzi
mesh_terms: D000071916:AC133 Antigen; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology* / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010455:Peptides / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism; D016019:Survival Analysis; D047368:Tumor Burden / Q000187:drug effects; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C116614:ABCG2 protein, human; D000071916:AC133 Antigen; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D015703:Antigens, CD; D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C527276:CXCR4 protein, human; D006023:Glycoproteins; D009363:Neoplasm Proteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D019718:Receptors, CXCR4; D002945:Cisplatin