Structural studies of an immunoenhancing water-soluble glucan isolated from hot water extract of an edible mushroom, Pleurotus florida, cultivar Assam Florida.
An immunoenhancing polysaccharide isolated from the hot water extract of the fruiting bodies of an edible mushroom Pleurotus florida, cultivar Assam Florida, was found to consist of only d-glucose as a monosaccharide constituent. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as This glucan stimulates macrophages, splenocytes, and thymocytes.
Roy
Sadhan K
SK
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Das
Debsankar
D
Mondal
Subhas
S
Maiti
Debabrata
D
Bhunia
Bibhas
B
Maiti
Tapas K
TK
Islam
Syed S
SS
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005936:Glucans / Q000737:chemistry* / Q000276:immunology*; D005947:Glucose / Q000032:analysis; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry*; D012995:Solubility; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D014867:Water
D005936:Glucans; D014867:Water; D005947:Glucose