One-step separation and purification of rupestonic acid and chrysosptertin B from Artemisia rupestris L. by high-speed counter-current chromatography.
BACKGROUND
Artemisia rupestris L. is a well-known traditional Chinese medicinal plant in Xinjiang. Rupestonic acid is the main active ingredient of A. rupestris L., and has been chosen as a 'marker compound' for the chemical evaluation or quality control of A. rupestris L. and its products. Although HSCCC separation method was developed before, the separation was performed with two steps using the same solvent system, which were time-consuming and waste of the solvents.


OBJECTIVE
To develop a simple HSCCC method for the separation and purification of rupestonic acid in a single run.


METHODS
The measurement of partition coefficient (K) was introduced to select the two-phase solvent system. The simple HSCCC method was established according to the selected solvent system for separation and purification of rupestonic acid. The purity of target compound was test by HPLC and the structure was identified by MS, (1)H NMR and (13)C NMR.


RESULTS
A total of 72.3 mg of rupestonic acid and 53.5 mg of chrysosptertin B with over 95% purity were yielded from 500 mg extracts of Artemisia rupestris L. in one-step separation.


CONCLUSIONS
The rupestonic acid was separated in a single run by HSCCC.
Yang
Yi
Y
Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
Gu
Dongyu
D
Yili
Abulimiti
A
Zhao
Yongxin
Y
He
Dajun
D
Aisa
Haji Akber
HA
D019306:Artemisia / Q000737:chemistry*; D052176:Azulenes / Q000032:analysis / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis / Q000302:isolation & purification*
D052176:Azulenes; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012717:Sesquiterpenes; C549910:chrysosptertin B; C057488:rupestonic acid