[The apoptosis of mouse macrophage J774A.1 induced by oxysterol depend on NF-kappaB activation].
OBJECTIVE
Investigated the relationship between NF-kappaB activation and cell apoptosis in mouse macrophages treated with 7-ketochesterol (7-KC).


METHODS
Cell apoptosis was detected by MTT assay, DNA fragmentation assay and flow cytometric analysis. NF-kappaB activation was detected by western blot and immunohistochemistry. Inhibitory assay was used to show the effect of the activation of NF-kappaB on the apoptosis induced by 7-KC.


RESULTS
7-KC inhibited macrophages proliferation, and then induced apoptosis, which is associated with NF-kappaB activation. Moreover, cell apoptosis with NF-kappaB activation was inhibited by pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-kappaB.


CONCLUSIONS
7-KC induced the activation of NF-kappaB and following cell apoptosis.
Huang
Zhen-Yu
ZY
Key Laboratory of Bio-organic Chemistry, College of Bioengineering, Dalian University, Dalian 116622, China. zhenyu_bio@hotmail.com
Liu
Qing-Ping
QP
Li
Wen-Zhe
WZ
Wang
Ren-Jun
RJ
Chi
Yan
Y
Liu
Yuan-Yuan
YY
Zhang
Qi-Ming
QM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D007653:Ketocholesterols / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D021381:Protein Transport
D007653:Ketocholesterols; D016328:NF-kappa B; C003001:7-ketocholesterol