title: Thorough evaluation of the validity of conventional enantio-gas chromatography in the analysis of volatile chiral compounds in mandarin essential oil: A comparative investigation with multidimensional gas chromatography.
abstract: The present research is focused on the determination of the enantiomeric distribution of chiral compounds, contained in mandarin essential oils, by means of conventional chiral gas chromatography with flame ionization detection (enantio-GC-FID); the results attained were compared with those derived from heart-cutting multidimensional GC-mass spectrometry (MDGC/MS), to evaluate the reliability of the monodimensional technique as a tool for quality control. The Deans-switch MDGC system was equipped with two GC ovens, which were connected via a heated transfer line, a flame ionization detector (FID1) in the first dimension and a quadrupole MS as second-dimension detector. The a priori knowledge of potential co-elutions concerning target compounds (an enantiomer and an interfering compound), when using enantio-GC-FID, could enable the use of corrected enantiomer excess values. Correction factors could be calculated through a preliminary GC-FID analysis (using an apolar column), considering the peak areas of the known interferences. The method used for the calculation of a so-called "coelution correction factor" is described, along with some examples.
authors.affiliation: Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, viale Annunziata, 98168 Messina, Italy.
authors.forename: Danilo
authors.initials: D
authors.lastname: Sciarrone
authors.forename: Luisa
authors.initials: L
authors.lastname: Schipilliti
authors.forename: Carla
authors.initials: C
authors.lastname: Ragonese
authors.forename: Peter Quinto
authors.initials: PQ
authors.lastname: Tranchida
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dugo
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
mesh_terms: D002957:Citrus / Q000737:chemistry*; D000081005:Cyclohexane Monoterpenes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism
chemical_list: D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; C028940:limonene-1,2-epoxide