title: Identification and characterization of two novel JARID1C mutations: suggestion of an emerging genotype-phenotype correlation.
abstract: Mental retardation (MR) is characterized by cognitive impairment with an IQ <70. Many of the major causes are genetically determined and the approximately 30% male excess suggests that mutations in genes carried on the X chromosome are disproportionably represented. One such gene, jumonji AT-rich interactive domain 1C (JARID1C) on Xp11.2, has been identified in families with X-linked MR (XLMR), with 18 different mutations reported to date. As part of a systematic resequencing of 720 genes in 208 XLMR families of the International Genetic of Learning Disability (IGOLD) consortium, two novel nucleotide changes in the JARID1C coding region were identified, with the nucleotide changes segregating with the disease phenotype in the two families. The first mutation is a single-nucleotide insertion in exon 21 (c.3258_3259insC p.K1087fs(*)43) causing a frameshift and resulting in a premature termination codon (PTC). Such PTC-containing mRNAs are generally degraded by nonsense-mediated mRNA decay (NMD) surveillance, but our results show that this is not the case with this mutation. The other change is a single-nucleotide substitution in exon 12 (c.1160C>A) in a published family with nonsyndromic MR, MRX13. This change occurs in a highly conserved amino acid, with proline (P) being substituted by threonine (T) (p.P554T). [corrected] Functional analysis shows that this amino-acid substitution compromises both tri- and didemethylase activity of the JARID1C protein. We conclude that the two novel changes impair JARID1C protein function and are disease-causing mutations in these families.
authors.affiliation: Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
authors.forename: Sinitdhorn
authors.initials: S
authors.lastname: Rujirabanjerd
authors.forename: John
authors.initials: J
authors.lastname: Nelson
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Tarpey
authors.forename: Anna
authors.initials: A
authors.lastname: Hackett
authors.forename: Sarah
authors.initials: S
authors.lastname: Edkins
authors.forename: F Lucy
authors.initials: FL
authors.lastname: Raymond
authors.forename: Charles E
authors.initials: CE
authors.lastname: Schwartz
authors.forename: Gillian
authors.initials: G
authors.lastname: Turner
authors.forename: Shigeki
authors.initials: S
authors.lastname: Iwase
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.forename: P Andrew
authors.initials: PA
authors.lastname: Futreal
authors.forename: Michael R
authors.initials: MR
authors.lastname: Stratton
authors.forename: Jozef
authors.initials: J
authors.lastname: Gecz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D056726:Genetic Association Studies; D056466:Histone Demethylases; D006657:Histones / Q000378:metabolism; D006801:Humans; D008297:Male; D008745:Methylation; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry / Q000235:genetics*; D010375:Pedigree; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D006657:Histones; D050505:Mutant Proteins; D056466:Histone Demethylases; C086877:KDM5C protein, human; D010089:Oxidoreductases, N-Demethylating