Antifibrotic activity of triterpenoids from the aerial parts of Euscaphis japonica on hepatic stellate cells.
In the course of screening antifibrotic activity of natural products employing HSC-T6, a rat hepatic stellate cell line, as an in vitro assay system, the methanolic extract of aerial parts of Euscaphis japonica (Tunb.) Kantiz (Staphyleaceae) showed significant inhibitory activity on HSC proliferation. Activity-guided fractionation led to the isolation of four triterpenoids, friedeline (1), glut-5-en-ol (2), pomolic acid (3), and methylrotundate (4). Among the triterpenoids isolated, pomolic acid (3) significantly inhibited the proliferation of HSCs at concentrations 10 and 100 microM.
Lee
Mi Kyeong
MK
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Lee
Ki Yong
KY
Jeon
Hee Young
HY
Sung
Sang Hyun
SH
Kim
Young Choong
YC
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005355:Fibrosis / Q000473:pathology; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019684:Magnoliopsida / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
D010936:Plant Extracts; D014315:Triterpenes; C115535:pomolic acid; D009828:Oleanolic Acid