title: Main fatty acid classes in vegetable oils by SB-ATR-Fourier transform infrared (FTIR) spectroscopy.
abstract: The prospect of using single bounce attenuated total reflectance (SB-ATR)-Fourier transform infrared (FTIR) spectroscopy as a rapid quantitative tool to determine the main fatty acid groups present in different edible oils was investigated. Partial least squares (PLS) calibrations were developed using SB-ATR-FTIR spectra which were associated with fatty acid groups (saturated, trans, mono- and polyunsaturated) using quantitative data obtained by gas chromatography (GC). Good calibrations were obtained for all main four fat groups (saturated, mono, trans and polyunsaturated) with excellent precision. The coefficient of determination (R(2)), root mean square error of prediction (RMSEP) and bias for validation set were obtained as 0.999, 2.43 and 0.998 for saturated; 0.999, 1.850 and 0.003 for mono; 0.999, 0.625 and -0.001 for trans while for poly the values were 0.999, 1.170 and 0.003, respectively. The results of 13 validation samples for total saturated, mono, trans and polyunsaturated fats by FI-IR were found in the range of 8.16-55.16, 37.62-74.75, 0.20-18.16 and 1.36-62.35%, respectively. The present study shows that it may well be possible to expand the utility of SB-ATR-FTIR spectroscopy not only to provide isolated trans data, but also serve as a simple, rapid and quantitative means of categorizing the main groups present in the edible oils. The information obtained would be useful for meeting the new lipid nutritional labeling requirements.
authors.affiliation: National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan. tufail.sherazi@yahoo.com
authors.forename: S T H
authors.initials: ST
authors.lastname: Sherazi
authors.forename: M Younis
authors.initials: MY
authors.lastname: Talpur
authors.forename: S A
authors.initials: SA
authors.lastname: Mahesar
authors.forename: Aftab A
authors.initials: AA
authors.lastname: Kandhro
authors.forename: Sarfraz
authors.initials: S
authors.lastname: Arain
mesh_terms: D002138:Calibration; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry; D016018:Least-Squares Analysis; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D044242:Trans Fatty Acids / Q000032:analysis / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D044242:Trans Fatty Acids