Effects of feeding and induction strategy on the production of BmR1 antigen in recombinant E. coli.
OBJECTIVE
To investigate the effects of feeding and induction strategies on the production of BmR1 recombinant antigen.


RESULTS
Fed-batch fermentation was studied with respect to the specific growth rate and mode of induction to assess the growth potential of the bacteria in a bioreactor and to produce high yield of BmR1 recombinant antigen. Cells were grown at a controlled specific growth rate (mu(set)) during pre-induction, followed by constant feeding postinduction. The highest biomass (24.3 g l(-1)) was obtained during fed-batch process operated at mu(set) of 0.15 h(-1), whereby lower mu(set) (0.075 h(-1)) gave the highest protein production (9.82 mg l(-1)). The yield of BmR1 was increased by 1.2-fold upon induction with 1 mmol l(-1) IPTG (isopropyl-beta-d-thiogalactoside) compared to using 5 mmol l(-1) and showed a further 3.5-fold increase when the culture was induced twice at the late log phase.


CONCLUSIONS
Combination of feeding at a lower mu(set) and twice induction with 1 mmol l(-1) IPTG yielded the best result of all variables tested, promising an improved method for BmR1 production.


CONCLUSIONS
This method can be used to increase the production scale of the BmR1 recombinant antigen to meet the increasing demand for Brugia Rapid(), a commercial diagnostic test for detection of brugian filariasis.
Norsyahida
A
A
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia.
Rahmah
N
N
Ahmad
R M Y
RM
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D017178:Brugia malayi / Q000276:immunology; D046508:Culture Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D000947:Antigens, Helminth; D011994:Recombinant Proteins