title: Monozygotic multiple gestation after intracytoplasmic sperm injection and preimplantation genetic diagnosis.
abstract: OBJECTIVE
  To report a possible association between intracytoplasmic sperm injection (ICSI)-preimplantation genetic diagnosis (PGD) and monozygotic multiple gestation.
  
  
  METHODS
  Small case series.
  
  
  METHODS
  In vitro fertilization unit in an academic medical center.
  
  
  METHODS
  Three patients were treated with ICSI-PGD for sexing as well as selection against a known translocation.
  
  
  METHODS
  Transfer of day 4 embryos to the uterus.
  
  
  METHODS
  Clinical pregnancy.
  
  
  RESULTS
  Two pairs of monozygotic twins and a triplet pregnancy.
  
  
  CONCLUSIONS
  Repeated manipulation of the zona pellucida as well as extended embryo culture during ICSI-PGD treatments may result in monozygotic twin and triplet pregnancies.
authors.affiliation: IVF Unit, Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel. kochman@hadassah.org.il
authors.forename: Ronit
authors.initials: R
authors.lastname: Haimov-Kochman
authors.forename: Hagit
authors.initials: H
authors.lastname: Daum
authors.forename: Francine
authors.initials: F
authors.lastname: Lossos
authors.forename: Einat
authors.initials: E
authors.lastname: Aizenman
authors.forename: Marion
authors.initials: M
authors.lastname: Werner
authors.forename: Simcha
authors.initials: S
authors.lastname: Yagel
authors.forename: Neri
authors.initials: N
authors.lastname: Laufer
authors.forename: Alex
authors.initials: A
authors.lastname: Simon
authors.forename: Arye
authors.initials: A
authors.lastname: Hurwitz
mesh_terms: D000328:Adult; D046149:Embryo Culture Techniques; D004624:Embryo Transfer / Q000009:adverse effects*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D011256:Pregnancy Outcome; D018607:Pregnancy Reduction, Multifetal; D019836:Preimplantation Diagnosis; D020554:Sperm Injections, Intracytoplasmic / Q000009:adverse effects*; D014310:Triplets; D014430:Twins, Monozygotic; D015044:Zona Pellucida