Retinoid signaling regulates breast cancer stem cell differentiation.
The cancer stem cell (CSC) hypothesis implicates the development of new therapeutic approaches to target the CSC population. Characterization of the pathways that regulate CSCs activity will facilitate the development of targeted therapies. We recently reported that the enzymatic activity of ALDH1, as measured by the ALDELFUOR assay, can be utilized to isolate normal and malignant breast stem cells in both primary tumors and cell lines. In this study, utilizing a tumorsphere assay, we have demonstrated the role of retinoid signaling in the regulation of breast CSCs self-renewal and differentiation. Utilizing the gene set enrichment analysis (GSEA) algorithm we identified gene sets and pathways associated with retinoid signaling. These pathways regulate breast CSCs biology and their inhibition may provide novel therapeutic approaches to target breast CSCs.
Ginestier
Christophe
C
Centre de Recherche en Cancérologie de Marseille, UMR891 Inserm/Institut Paoli-Calmettes, Marseilles, France. christophe.ginestier@inserm.fr
Wicinski
Julien
J
Cervera
Nathalie
N
Monville
Florence
F
Finetti
Pascal
P
Bertucci
François
F
Wicha
Max S
MS
Birnbaum
Daniel
D
Charafe-Jauffret
Emmanuelle
E
D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D001943:Breast Neoplasms / Q000188:drug therapy*; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000201:enzymology; D050697:Retinal Dehydrogenase; D012176:Retinoids / Q000494:pharmacology*; D015398:Signal Transduction; D014212:Tretinoin / Q000494:pharmacology
D007527:Isoenzymes; D012176:Retinoids; D014212:Tretinoin; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase