title: Gold and silver nanoparticles conjugated with heparin derivative possess anti-angiogenesis properties.
abstract: Silver and gold nanoparticles display unique physical and biological properties that have been extensively studied for biological and medical applications. Typically, gold and silver nanoparticles are prepared by chemical reductants that utilize excess toxic reactants, which need to be removed for biological purposes. We utilized a clean method involving a single synthetic step to prepare metal nanoparticles for evaluating potential effects on angiogenesis modulation. These nanoparticles were prepared by reducing silver nitrate and gold chloride with diaminopyridinyl (DAP)-derivatized heparin (HP) polysaccharides. Both gold and silver nanoparticles reduced with DAPHP exhibited effective inhibition of basic fibroblast growth factor (FGF-2)-induced angiogenesis, with an enhanced anti-angiogenesis efficacy with the conjugation to DAPHP (P<0.01) as compared to glucose conjugation. These results suggest that DAPHP-reduced silver nanoparticles and gold nanoparticles have potential in pathological angiogenesis accelerated disorders such as cancer and inflammatory diseases.
authors.affiliation: Department of Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Kemp
authors.forename: Ashavani
authors.initials: A
authors.lastname: Kumar
authors.forename: Shaymaa
authors.initials: S
authors.lastname: Mousa
authors.forename: Evgeny
authors.initials: E
authors.lastname: Dyskin
authors.forename: Murat
authors.initials: M
authors.lastname: Yalcin
authors.forename: Pulickel
authors.initials: P
authors.lastname: Ajayan
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
authors.forename: Shaker A
authors.initials: SA
authors.lastname: Mousa
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D017612:Gold Compounds / Q000737:chemistry; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry
chemical_list: D020533:Angiogenesis Inhibitors; D017612:Gold Compounds; D016222:Fibroblast Growth Factor 2; C038016:gold chloride; D012834:Silver; D006046:Gold; D006493:Heparin; D012835:Silver Nitrate