title: 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans.
abstract: Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade.
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Crystale Siew-Ying
authors.initials: CS
authors.lastname: Lim
authors.forename: Won-Fen
authors.initials: WF
authors.lastname: Wong
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.forename: Kee-Peng
authors.initials: KP
authors.lastname: Ng
authors.forename: Heng-Fong
authors.initials: HF
authors.lastname: Seow
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Chong
mesh_terms: D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D007851:Dodecanol / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development*; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D005656:Fungal Proteins; D007851:Dodecanol; D056565:Sirtuin 2