title: Durable mesenchymal stem cell labelling by using polyhedral superparamagnetic iron oxide nanoparticles.
abstract: Small polyhedral superparamagnetic iron oxide (SPIO) nanoparticles (<10 nm) coated with a thin layer of silica were prepared (SPIO@SiO(2) and SPIO@SiO(2)-NH(2)). Surface modification of the small polyhedral silica-coated SPIO nanoparticles with amines led to substantially higher mesenchymal stem cell (MSC) labelling efficiency without the use of additional transfecting agents. Therefore, amine surface-modified nanoparticles (SPIO@ SiO(2)-NH(2)) appeared to be the preferred candidate for MSC labelling. In vitro studies demonstrated that controlled labelling of SPIO@SiO(2) and SPIO@SiO(2)-NH(2) did not cause MSC death or proliferation inhibition. MSCs labelled with SPIO@SiO(2)-NH(2) nanoparticles retained differentiation potential and showed osteogenic, adipogenic and chondrogenic differentiations. The noncytotoxic polyhedral SPIO@SiO(2)-NH(2) nanoparticle-labelled MSCs were successfully implanted in rabbit brain and erector spinae muscle, and demonstrated long-lasting, durable MRI labelling efficacy after 8-12 weeks.
authors.affiliation: Department of Diagnostic Radiology and Organ Imaging, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
authors.forename: Hao-Hao
authors.initials: HH
authors.lastname: Wang
authors.forename: Yi-Xiang J
authors.initials: YX
authors.lastname: Wang
authors.forename: Ken Cham-Fai
authors.initials: KC
authors.lastname: Leung
authors.forename: Doris W T
authors.initials: DW
authors.lastname: Au
authors.forename: Shouhu
authors.initials: S
authors.lastname: Xuan
authors.forename: Chun-Pong
authors.initials: CP
authors.lastname: Chak
authors.forename: Simon K M
authors.initials: SK
authors.lastname: Lee
authors.forename: Hui
authors.initials: H
authors.lastname: Sheng
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: James F
authors.initials: JF
authors.lastname: Griffith
authors.forename: Anil T
authors.initials: AT
authors.lastname: Ahuja
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D003287:Contrast Media / Q000737:chemistry* / Q000493:pharmacokinetics; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D007501:Iron / Q000493:pharmacokinetics*; D008279:Magnetic Resonance Imaging / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053758:Nanoparticles; D011817:Rabbits; D011990:Receptors, Transferrin / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000588:Amines; D003287:Contrast Media; D011990:Receptors, Transferrin; D012822:Silicon Dioxide; D007501:Iron; D052203:Ferrosoferric Oxide