Evaluation of the expression and protective potential of Leptospiral sphingomyelinases.
Leptospirosis is a zoonotic disease of global distribution, which affects both animals and humans. Pathogenic leptospires, the bacteria that cause this disease, require iron for their growth, and these spirochetes probably use their hemolysins, such as the sphingomyelinases, as a way to obtain this important nutrient from host red blood cells during infection. We expressed and purified the leptospiral sphingomyelinases Sph1, Sph2, Sph4, and SphH in a heterologous system. However, the recombinant proteins were not able to lyse sheep erythrocytes, despite having regular secondary structures. Transcripts for all sphingomyelinases tested were detected by RT-PCR analyses, but only Sph2 and SphH native proteins could be detected in Western blot assays using Leptospira whole extracts as well as in renal tubules of infected hamsters. Moreover, antibodies present in the serum of a human patient with laboratory-confirmed leptospirosis recognized Sph2, indicating that this sphingomyelinase is expressed and exposed to the immune system during infection in humans. However, in an animal challenge model, none of the sphingomyelinases tested conferred protection against leptospirosis.
Carvalho
Eneas
E
Centro de Biotecnologia, Instituto Butantan, São Paulo, SP, Brazil.
Barbosa
Angela S
AS
Gómez
Ricardo M
RM
Oliveira
Maria L S
ML
Romero
Eliete C
EC
Gonçales
Amane P
AP
Morais
Zenaide M
ZM
Vasconcellos
Sílvio A
SA
Ho
Paulo L
PL
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D006224:Cricetinae; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007921:Leptospira interrogans / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D007922:Leptospirosis / Q000276:immunology* / Q000382:microbiology; D012756:Sheep; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics / Q000276:immunology*
D001426:Bacterial Proteins; D013108:Sphingomyelin Phosphodiesterase