title: Induction and cellular expression of tartrate resistant acid phosphatase during dextran sodium sulphate induced colitis in rats.
abstract: The aim of this study was to investigate the cellular and molecular expression of tartrate resistant acid phosphatase (TRAP) as a marker of activated macrophages in macrophage dependent dextran sulphate sodium (DSS)-induced colitis in rats. In normal colon, TRAP+/CX(3)CR(1)+ macrophages were located in the upper part of the lamina propria. In the early stage (day 1-3) of acute colitis prior to histopathological changes, induction of the cytokines TNFalpha, IL-12 and IFN gamma occurred concomitant with increased mRNA and enzyme activity of TRAP along with a slight increase of TRAP immunolabelling in macrophages of the upper lamina propria, suggesting induction of TRAP in resident macrophages. Among these cytokines, TNFalpha up-regulated TRAP expression in the RAW 264.7 monocyte/macrophage cell line. In a later phase (day 7) with fulminant colitis, a massive infiltration of macrophages including recruited TRAP+/CCR2+ cells was observed also in the lower part of the lamina propria as well as in the submuscular layer. Additionally, differentiated cellular expression of pro- and mature TRAP also suggest that mucosal macrophages in the lower part of lamina propria bordering the sub-mucosa provide a source of replenishment of macrophages situated in the upper lamina propria. In conclusion, induction of TRAP provides an early sign of macrophage responsiveness in DSS induced colitis.
authors.affiliation: Division of Pathology F46, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86, Stockholm, Sweden.
authors.forename: Pernilla
authors.initials: P
authors.lastname: Lång
authors.forename: Stefan
authors.initials: S
authors.lastname: Lange
authors.forename: Dick
authors.initials: D
authors.lastname: Delbro
authors.forename: Göran
authors.initials: G
authors.lastname: Andersson
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics*; D000818:Animals; D015415:Biomarkers; D003092:Colitis / Q000139:chemically induced / Q000276:immunology*; D003106:Colon / Q000276:immunology; D016207:Cytokines / Q000235:genetics; D016264:Dextran Sulfate; D007527:Isoenzymes / Q000235:genetics*; D008262:Macrophage Activation; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D000071681:Tartrate-Resistant Acid Phosphatase; D015533:Transcriptional Activation / Q000276:immunology*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D007527:Isoenzymes; D012333:RNA, Messenger; D016264:Dextran Sulfate; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase