title: Variability of histopathological changes in childhood celiac disease.
abstract: OBJECTIVE
  Adult studies of celiac disease (CD) have shown that duodenal mucosal histopathological changes may be patchy, and the diagnostic utility of duodenal bulb biopsies is believed to be limited. Few related pediatric data exist.
  
  
  METHODS
  We assessed the prevalence of variable biopsy findings and duodenal bulb involvement in children with CD, as well as its association with clinical parameters. A total of 198 consecutive cases of CD diagnosed at the Children's Hospital during 2001-2005 were analyzed. All biopsies were scored by a pathologist blinded to the clinical data using the Marsh criteria. Mucosal changes were classified as focal if changes consistent with CD and normal mucosa were found within a single biopsy fragment. Patchiness was defined as variation of at least one Marsh grade between separate fragments in a biopsy set.
  
  
  RESULTS
  The median age was 9.3 years; 62% were female. An average of 3.6 biopsy samples was obtained per case. In 101 cases, biopsy samples were obtained from the duodenal bulb and the second portion of the duodenum. Focality was present in biopsy samples collected from 36 (18%) cases. Patchiness was found in 105 (53%) cases, and at least 1 normal biopsy fragment was present in 71 (36%) cases. In 10 cases, only the bulb biopsies were diagnostic of CD. There was no association with the clinical features examined.
  
  
  CONCLUSIONS
  Duodenal involvement in pediatric CD is frequently patchy and may show variable severity even within a single biopsy fragment. Variability cannot be predicted by clinical characteristics. Multiple endoscopic biopsies, including the duodenal bulb, should be obtained in suspected pediatric CD cases to maximize diagnostic yield.
authors.affiliation: Department of Pathology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. dascha.weir@childrens.harvard.edu
authors.forename: Dascha C
authors.initials: DC
authors.lastname: Weir
authors.forename: Jonathan N
authors.initials: JN
authors.lastname: Glickman
authors.forename: Tracey
authors.initials: T
authors.lastname: Roiff
authors.forename: Clarissa
authors.initials: C
authors.lastname: Valim
authors.forename: Alan M
authors.initials: AM
authors.lastname: Leichtner
mesh_terms: D001706:Biopsy; D002446:Celiac Disease / Q000453:epidemiology / Q000473:pathology*; D016009:Chi-Square Distribution; D002648:Child; D004386:Duodenum / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D015995:Prevalence; D018709:Statistics, Nonparametric