A method to immunolabel rodent spinal cord neurons and glia for molecular study in specific laser microdissected cells involved in neurodegenerative disorders.
Neuron-glia interaction is involved in physiological function of neurons, however, recent evidences have suggested glial cells as participants in neurotoxic and neurotrophic mechanisms of neurodegenerative/neuroregenerative processes. Laser microdissection offers a unique opportunity to study molecular regulation in specific immunolabeled cell types. However, an adequate protocol to allow morphological and molecular analysis of rodent spinal cord astrocyte, microglia and motoneurons remains a big challenge. In this paper we present a quick method to immunolabel those cells in flash frozen sections to be used in molecular biology analyses after laser microdissection and pressure catapulting.
de Oliveira
Gabriela Pintar
GP
Neuroregeneration Center, Department of Neurology, University of São Paulo School of Medicine, São Paulo, SP 01246-903, Brazil.
Maximino
Jessica Ruivo
JR
Lin
Chin Jia
CJ
Chadi
Gerson
G
D000818:Animals; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007834:Lasers; D008297:Male; D051379:Mice; D042282:Microdissection; D019636:Neurodegenerative Diseases / Q000473:pathology*; D009457:Neuroglia / Q000473:pathology*; D009474:Neurons / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*