[Research on prerequisite for using bovine hemoglobin modified by polyethylene glycol as a red blood cell substitute].
This paper aims to raise concerns about the modification of bovine hemoglobin with the use of SC-mPEG as modifying reagent. The yield rates of SC-mPEG-BHB under dissimilar conditions are studied, and more important, a series of methods used to characterize the SC-mPEG-BHB mixtures are built. The best reactive system has been confirmed, i.e., at 4 degrees C, pH9; SC-mPEG and BHB are mixed according to the proportion of 60:1 and be submitted to reaction for 2 h.
Sun
Jufeng
J
Pharmaceutical College of Binzhou Medical College, Yantai 264003, China. sjf.xzy@163.com
Liu
Yunyan
Y
D000818:Animals; D001802:Blood Substitutes / Q000138:chemical synthesis; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry* / Q000145:classification / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000145:classification
D001802:Blood Substitutes; D006454:Hemoglobins; C109960:PEG-hemoglobin; C099846:succinimidyl carbonate poly(ethylene glycol) monomethyl ether; D011092:Polyethylene Glycols