The keratin-related Ouroboros proteins function as immune antigens mediating tail regression in Xenopus metamorphosis.
Tail resorption during amphibian metamorphosis has been thought to be controlled mainly by a cell-autonomous mechanism of programmed cell death triggered by thyroid hormone. However, we have proposed a role for the immune response in metamorphosis, based on the finding that syngeneic grafts of tadpole tail skin into adult Xenopus animals are rejected by T cells. To test this, we identified two tail antigen genes called ouro1 and ouro2 that encode keratin-related proteins. Recombinant Ouro1 and Ouro2 proteins generated proliferative responses in vitro in T cells isolated from naive adult Xenopus animals. These genes were expressed specifically in the tail skin at the climax of metamorphosis. Overexpression of ouro1 and ouro2 induced T-cell accumulation and precocious tail degeneration after full differentiation of adult-type T cells when overexpressed in the tail region. When the expression of ouro1 and ouro2 were knocked down, tail skin tissue remained even after metamorphosis was complete. Our findings indicate that Ouro proteins participate in the process of tail regression as immune antigens and highlight the possibility that the acquired immune system contributes not only to self-defense but also to remodeling processes in vertebrate morphogenesis.
Mukaigasa
Katsuki
K
Department of Biology, Faculty of Science, Niigata University, Niigata 950-2181, Japan.
Hanasaki
Akira
A
Maéno
Mitsugu
M
Fujii
Hiroshi
H
Hayashida
Shin-ichiro
S
Itoh
Mari
M
Kobayashi
Makoto
M
Tochinai
Shin
S
Hatta
Masayuki
M
Iwabuchi
Kazuya
K
Taira
Masanori
M
Onoé
Kazunori
K
Izutsu
Yumi
Y
D000818:Animals; D030801:Animals, Genetically Modified; D000941:Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D018507:Gene Expression Regulation, Developmental; D007633:Keratins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D012867:Skin / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013623:Tail / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D014158:Transcription, Genetic; D029867:Xenopus Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism*
D000941:Antigens; C544963:Ouro1 protein, Xenopus; C544964:Ouro2 protein, Xenopus; D029867:Xenopus Proteins; D007633:Keratins