Independent recruitment of F box genes to regulate hermaphrodite development during nematode evolution.
Elucidating the molecular mechanisms that created ancient complex traits like insect wings is difficult. Fortunately, some complex traits have arisen recently. For example, hermaphroditic reproduction evolved independently many times during recent nematode evolution. Although C. elegans hermaphrodites require fog-2, which encodes an F box protein that regulates the translation of tra-2 mRNAs, the related species C. briggsae lacks fog-2. We identified a critical regulator of hermaphrodite development in C. briggsae, named she-1. Analysis of double mutants indicates that she-1 acts upstream of tra-2 in C. briggsae, just as fog-2 does in C. elegans. Molecular cloning shows that she-1 encodes a novel F box protein that was created by a recent gene duplication. Whereas FOG-2 acts through GLD-1 in C. elegans, SHE-1 does not bind GLD-1 in C. briggsae. Thus, both species recruited F box genes produced by recent duplication events into the sex-determination pathway to control hermaphrodite development, but these genes have distinct activities. This result implies that some gene families are more likely to give rise to novel regulatory genes than other families. Finally, we note that null mutations of she-1 are temperature sensitive, so C. briggsae might once have been a facultative hermaphrodite.
Guo
Yiqing
Y
Department of Molecular Biology, School of Osteopathic Medicine, The University of Medicine and Dentistry of New Jersey, B303 Science Center, 2 Medical Center Drive, Stratford, NJ 08084, USA.
Lang
Shirley
S
Ellis
Ronald E
RE
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D002107:Caenorhabditis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D012734:Disorders of Sex Development / Q000235:genetics; D044783:F-Box Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020440:Gene Duplication; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism; D016415:Sequence Alignment; D019849:Sex Determination Processes; D013091:Spermatogenesis / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D020798:Two-Hybrid System Techniques
D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D044783:F-Box Proteins; C094448:GLD-1 protein, C elegans; D015801:Helminth Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; C492171:fog-2 protein, C elegans; C076107:tra-2 protein, C elegans; D044843:SKP Cullin F-Box Protein Ligases