Inactivation of the CTD phosphatase-like gene OsCPL1 enhances the development of the abscission layer and seed shattering in rice.
Although susceptibility to seed shattering causes severe yield loss during cereal crop harvest, it is an adaptive trait for seed dispersal in wild plants. We previously identified a recessive shattering locus, sh-h, from the rice shattering mutant line Hsh that carries an enhanced abscission layer. Here, we further mapped sh-h to a 34-kb region on chromosome 7 by analyzing 240 F(2) plants and five F(3) lines from the cross between Hsh and Blue&Gundil. Hsh had a point mutation at the 3' splice site of the seventh intron within LOC_Os07g10690, causing a 15-bp deletion of its mRNA as a result of altered splicing. Two transferred DNA (T-DNA) insertion mutants and one point mutant exhibited the enhanced shattering phenotype, confirming that LOC_Os07g10690 is indeed the sh-h gene. RNA interference (RNAi) transgenic lines with suppressed expression of this gene exhibited greater shattering. This gene, which encodes a protein containing a conserved carboxy-terminal domain (CTD) phosphatase domain, was named Oryza sativa CTD phosphatase-like 1 (OsCPL1). Subcellular localization and biochemical analysis revealed that the OsCPL1 protein is a nuclear phosphatase, a common characteristic of metazoan CTD phosphatases involved in cell differentiation. These results demonstrate that OsCPL1 represses differentiation of the abscission layer during panicle development.
Ji
Hyeonso
H
Department of Agricultural Bio-resources, National Academy of Agricultural Science (NAAS), Suwon 441-707, Korea.
Kim
Sung-Ryul
SR
Kim
Yul-Ho
YH
Kim
Hakbum
H
Eun
Moo-Young
MY
Jin
Il-Doo
ID
Cha
Young-Soon
YS
Yun
Doh-Won
DW
Ahn
Byung-Ohg
BO
Lee
Myung Chul
MC
Lee
Gang-Seob
GS
Yoon
Ung-Han
UH
Lee
Jung-Sook
JS
Lee
Yeon-Hee
YH
Suh
Seok-Cheol
SC
Jiang
Wenzhu
W
Yang
Jung-Il
JI
Jin
Ping
P
McCouch
Susan R
SR
An
Gynheung
G
Koh
Hee-Jong
HJ
D000595:Amino Acid Sequence; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics / Q000254:growth & development*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D017354:Point Mutation / Q000235:genetics; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development*; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C036483:T-DNA; D049452:Green Fluorescent Proteins; D010749:Phosphoprotein Phosphatases