Infection kinetics of human adenovirus serotype 41 in HEK 293 cells.
The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.
Siqueira-Silva
Joselma
J
Laboratório de Biologia Molecular de Adenovírus, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Yeda
Fernanda Perez
FP
Favier
Anne-Laure
AL
Mezin
Paulette
P
Silva
Misael Leonardo
ML
Barrella
Karina Médici
KM
Mehnert
Dolores Ursula
DU
Fender
Pascal
P
Hársi
Charlotte Marianna
CM
D000260:Adenoviruses, Human / Q000145:classification / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line / Q000821:virology; D002999:Clone Cells; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D011817:Rabbits; D013997:Time Factors