title: Molecular cloning and characterization of a class II ADP ribosylation factor from the shrimp Marsupenaeus japonicus.
abstract: In shrimp, small GTPases in the Ras superfamily can regulate hemolytic phagocytosis against WSSV infection. However, the ADP ribosylation factors (Arfs), also belonging to the regulatory GTP-binding proteins and playing a central role in membrane trafficking, have not been reported yet in shrimp and their relationship with WSSV infection is completely unknown to date. Here, a novel class II Arf (designated as MjArf4) was cloned and characterized from the shrimp Marsupenaeus japonicus. Like other Arfs, MjArf4 contains an N-terminal myristoylated site, a p loop, switch regions, as well as an interswitch region. In High Five cells, when MjArf4 was in its GDP-bound form, it dispersed into the whole cell, whereas in the GTP-bound form it promoted formation of a punctuate Golgi-like structure, indicating that the MjArf4 distribution was dependent on its GDP/GTP binding. After challenge with WSSV, the mRNA level of MjArf4 was up-regulated significantly as WSSV propagated. Thus, a member of the Arf family was characterized for the first time in shrimp and found to be involved in WSSV infection.
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Mingchang
authors.initials: M
authors.lastname: Zhang
authors.forename: Jinyou
authors.initials: J
authors.lastname: Ma
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Lei
authors.forename: Xun
authors.initials: X
authors.lastname: Xu
mesh_terms: D020727:ADP-Ribosylation Factors / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000276:immunology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology / Q000235:genetics / Q000276:immunology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046848:White spot syndrome virus 1 / Q000276:immunology
chemical_list: D020727:ADP-Ribosylation Factors