title: Molecular epidemiology of Brazilian pseudorabies viral isolates.
abstract: Pseudorabies is a disease caused by pseudorabies virus (PRV) and is responsible for considerable economic losses in the swine industry. The objective of this work was to use molecular epidemiology as a tool to facilitate the study of PRV outbreaks in Brazil. The standard PRV strain Shope, the vaccine strain Bartha and isolates from the south and the southeast regions of Brazil, were amplified for gE and gC partial genes by PCR. Results indicated that Brazilian PRV isolates are grouped in two clusters, A and B, except for one isolate that grouped with Bartha and Shope. Most Brazilian PRV isolates belonged to cluster B and diverged from virus isolated from other countries.
authors.affiliation: Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG, Brazil. augustofj@yahoo.com.br
authors.forename: Antônio Augusto
authors.initials: AA
authors.lastname: Fonseca
authors.forename: Marcelo Fernandes
authors.initials: MF
authors.lastname: Camargos
authors.forename: Anapolino Macedo
authors.initials: AM
authors.lastname: de Oliveira
authors.forename: Janice R
authors.initials: JR
authors.lastname: Ciacci-Zanella
authors.forename: Maria Aparecida C
authors.initials: MA
authors.lastname: Patrício
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Braga
authors.forename: Eliane S
authors.initials: ES
authors.lastname: Cunha
authors.forename: Régia
authors.initials: R
authors.lastname: D'Ambros
authors.forename: Marcos Bryan
authors.initials: MB
authors.lastname: Heinemann
authors.forename: Rômulo Cerqueira
authors.initials: RC
authors.lastname: Leite
authors.forename: Jenner K Pimenta
authors.initials: JK
authors.lastname: dos Reis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011557:Pseudorabies / Q000453:epidemiology* / Q000821:virology*; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1; C038535:glycoprotein gC, herpes simplex virus type 1