[Participation of Ca2+ -ATPase in calcium homeostasis of cerebellar neurons in crucian carp].
In our experiments the participation ofendoplasmic reticulum Ca2+ -ATPase (SERCA) was studied as a separate intracellular participant of calcium homeostasis in neurons of cerebellum from hypoxia-tolerant fish species--crucian Carassius gibelio. The SERCA's blockers cyclopiazonic acid and tapsigargin were used. Intracellular Ca2+ concentration ([Ca2+]i) was measured by Ca2+ -sensitive dye Fura-2AM and microfluorescent method for measuring free Ca2+ concentration. We established that cerebellar neurons ofcrucian have a well expressed system to clean the cytoplasm from Ca2+ overflow that is presented by SERCA-pump ofendoplasmic reticulum. The switching off a SERCA-pump by means of its selective blockers results in increase of the Ca2+ -transient amplitude and Ca2+ entrance in the cell approximately by 20%-100% in comparison to control depending on duration of depolarization. It is assumed, that SERCA-pump ofcrucian neurons like in mammals substantially affects temporary and amplitude characteristics of calcium signals in neurons and plays a considerable role in cleaning of cytoplasm from Ca2+ during functional activity of the cell.
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D000222:Adaptation, Physiological; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002347:Carps / Q000378:metabolism / Q000502:physiology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry; D016257:Fura-2 / Q000031:analogs & derivatives / Q000737:chemistry; D006706:Homeostasis / Q000502:physiology*; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*
D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; C049925:fura-2-am; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium; D016257:Fura-2