[Experimental study on hydrogel dressing in vein injury prevention and treatment].
OBJECTIVE
To observe the efficacy of hydrogel dressings in preventing and treating vein injury of rabbits so as to provide a experimental evidence for clinical application.


METHODS
Twenty-four healthy large-eared Japanese rabbits (48 ears) were chosen, weighing (2.15 +/- 0.15) kg, and divided into 3 groups randomly. The vein injury models were made by intravenously infusing 20% mannite (2.5 mL/kg). The sites of puncture were treated with hydrogel dressings (group A, n=8) and 25% MgSO4 (group B, n=8) 5 minutes after infusion. The sites of puncture were not treated as a blank control (group C, n=8). The tissue specimens were collected from the auricular veins at 24 hours after mannite infusion for histological observation by HE staining. The injury of the vessel wall, hemorrhage around the vessels, infiltration of inflammatory cells, and disturbance of circulation were observed to evaluate the injury degree of vein.


RESULTS
There existed redness and congestion in the injured veins of each group. HE staining showed that in both groups A and B, the vessel wall was slightly injured and hemorrhage around the vessel was mild. There existed infiltration of inflammatory cells in the vessel wall and surrounding tissues. There also existed congestion and thrombus in the vessel lumen in these two groups. While in group C, the injury of vessel wall was severe, and schistic bleeding in the surrounding tissue of the vessel was existed. The severe congestion and thrombus in the vessel lumen was observed. There was no significant difference among three groups in the extent of vein wall injury and hemorrhage around the vessel (P > 0.05). The degree of infiltration of inflammatory cells and circulatory disturbance in both groups A and B were significantly less than that of group C (P < 0.05); but there was no significant difference between groups A and B (P > 0.05).


CONCLUSIONS
Hydrogel dressing is helpful to prevent vein injury of rabbits induced by mannite.
Chen
Zhonglan
Z
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China.
Yang
Xiaochun
X
Ning
Ning
N
Yin
Haillin
H
Zhang
Qiang
Q
Cheng
Weibo
W
D000818:Animals; D047070:Bandages, Hydrocolloid; D005260:Female; D007262:Infusions, Intravenous; D008297:Male; D011817:Rabbits; D014680:Veins / Q000293:injuries*