Measurement uncertainty in analytical studies based on surface plasmon resonance.
The sensitivity of surface plasmon resonance (SPR) transducers depends on the thickness and spatial organization of interfacial structures at their surfaces. This is because the response of the SPR sensor is determined by integrating the distance-dependent refractive index (spatial interfacial architectures), weighted by the square of the electromagnetic field, from zero to infinite distance. The effect of SPR transducer sensitivity variation on the accuracy of SPR analysis is considered. Our quantitative estimation (based on the results of refractometric studies) gave a value for sensitivity variation of about 3% for the formation of a self-assembled thiocyanate layer or a trypsin-soybean trypsin inhibitor surface complex. The estimated accuracy in measured variation (i.e., by 0.01) for the refractive index of the external medium was 3x10(-4). This restriction, which follows immediately from the physical mechanism of the SPR phenomenon, should be taken into account when analyzing data obtained with the above technique.
Boltovets
Praskovia M
PM
V. Lashkaryov Institute of Semiconductor Physics, NAS Ukraine, Kyiv, Ukraine.
Snopok
Boris A
BA
D000465:Algorithms; D002138:Calibration; D008962:Models, Theoretical; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D014159:Transducers / Q000592:standards