The water-deficit stress- and red-rot-related genes in sugarcane.
Sugarcane is an important international commodity as a valuable agricultural crop especially in developing countries. Sequencing was carried out to generate >35,000 expressed sequence tags (ESTs) from healthy as well as red-rot-infected tissue of Indian subtropical variety of sugarcane. Subsequent clustering with existing sugarcane ESTs in public databases identified 4,087 clusters, including 85 clusters that preferentially express upon Colletotrichum falcatum (red-rot) infection, which were previously unreported. Real-time reverse transcription-PCR profiling of selected EST clusters identified several sugarcane clusters that show differential expression in response to biotic and abiotic stress conditions. Twenty-five stress-related clusters showed >2-fold relative expression during water-deficit stress in sugarcane. Similarly, EST clusters could be identified, which exhibit association with red-rot disease when assessed in red-rot-susceptible and red-rot-resistant varieties of sugarcane. Such EST clusters are good candidates for in-depth analysis to elucidate stress-responsive pathways in sugarcane and facilitate genetic manipulation to tailor this crop for tolerance to various stresses.
Gupta
Vikrant
V
Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110 021, India.
Raghuvanshi
Saurabh
S
Gupta
Ambika
A
Saini
Navin
N
Gaur
Anupama
A
Khan
M S
MS
Gupta
R S
RS
Singh
J
J
Duttamajumder
S K
SK
Srivastava
S
S
Suman
A
A
Khurana
Jitendra P
JP
Kapur
Raman
R
Tyagi
Akhilesh K
AK
D001483:Base Sequence; D016000:Cluster Analysis; D020231:Colletotrichum / Q000502:physiology*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D007700:Kinetics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031786:Saccharum / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics*; D014867:Water / Q000378:metabolism*
D014867:Water