title: Synthesis and characterization of hydroxyl-functionalized caprolactone copolymers and their effect on adhesion, proliferation, and differentiation of human mesenchymal stem cells.
abstract: The aim of this study was to develop new hydrophilic polyesters for tissue engineering applications. In our approach, poly(benzyloxymethyl glycolide-co-epsilon-caprolactone)s (pBHMG-CLs) were synthesized through melt copolymerization of epsilon-caprolactone (CL) and benzyl-protected hydroxymethyl glycolide (BHMG). Deprotection of the polymers yielded copolymers with pendant hydroxyl groups, poly(hydroxymethylglycolide-co-epsilon-caprolactone) (pHMG-CL). The synthesized polymers were characterized by GPC, NMR, and DSC techniques. The resulting copolymers consisting of up to 10% of HMG monomer were semicrystalline with a melting temperature above body temperature. Water contact angle measurements of polymeric films showed that increasing HMG content resulted in higher surface hydrophilicity, as evidenced from a decrease in receding contact angle from 68 degrees for PCL to 40 degrees for 10% HMG-CL. Human mesenchymal stem cells showed good adherence onto pHMG-CL films as compared to the more hydrophobic PCL surfaces. The cells survived and were able to differentiate toward osteogenic lineage on pHMG-CL surfaces. This study shows that the aforementioned hydrophilic polymers are attractive candidates for the design of scaffolds for tissue engineering applications.
authors.affiliation: Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Faculty of Science, Utrecht University, Utrecht, The Netherlands.
authors.forename: Hajar
authors.initials: H
authors.lastname: Seyednejad
authors.forename: Tina
authors.initials: T
authors.lastname: Vermonden
authors.forename: Natalja E
authors.initials: NE
authors.lastname: Fedorovich
authors.forename: Roel
authors.initials: R
authors.lastname: van Eijk
authors.forename: Mies J
authors.initials: MJ
authors.lastname: van Steenbergen
authors.forename: Wouter J A
authors.initials: WJ
authors.lastname: Dhert
authors.forename: Cornelus F
authors.initials: CF
authors.lastname: van Nostrum
authors.forename: Wim E
authors.initials: WE
authors.lastname: Hennink
mesh_terms: D002208:Caproates / Q000138:chemical synthesis* / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D017665:Hydroxyl Radical / Q000138:chemical synthesis* / Q000737:chemistry; D007783:Lactones / Q000138:chemical synthesis* / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D002208:Caproates; D007783:Lactones; D011108:Polymers; D017665:Hydroxyl Radical; C121056:caprolactone