[Study on cryopreservation of tissue engineered tendon by vitrification].
In search of a practical method for the cryopreservation of tissue engineered tendon (TET) by vitrification, we adopted 3 kinds of different cryoprotective agents (CPA)(21% DMSO, DP6 and VS55) in studying the freeze-stored effect of different CPA. The cellular morphology and post-thaw viability of the TET were examined by scanning electron microscopy (SEM), flow cytometry, and confocal laser microscopy (CLM). The results showed that there existed statistically significant difference in respect to the post-thaw viability between 21% DMSO and DP6, VS55; The cells specially adhered to the surface of scaffold both before or after cryopreservation by use of 21% DMSO. It was suggested that 21% DMSO as a CPA for TET cryopreservation was better than DP6 and VS55 in the current study.
Liu
Chengjun
C
Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Qin
Tingwu
T
Wang
Zhi
Z
Chen
Xi
X
Yang
Zhiming
Z
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000032:analysis*; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013710:Tendons / Q000166:cytology / Q000637:transplantation; D023822:Tissue Engineering; D014021:Tissue Preservation / Q000379:methods*; D054457:Tissue Scaffolds; D058989:Vitrification
D003451:Cryoprotective Agents