Expression of interleukin-15 and its receptor on the surface of stimulated human umbilical vein endothelial cells.
Human interleukin-15 (hIL-15) is an important cytokine to activate endothelial cells and can be regulated by many other cytokines. The aim of this study is to examine the ability of interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) to induce the production of human interleukin-15 (hIL-15) and IL-15 receptor (IL-15Ralpha) by human umbilical vein endothelial cells (HUVECs). The data are summarized as follows: 1. Northern blot revealed that IL-15 mRNA was up-regulated by IFN-gamma and TNF-alpha. 2. Intracellular IL-15 protein was visualized by fluorescence microscopy, whereas the expression of IL-15 on the surface of HUVECs was detected by fluorescence activated cell sorting (FACS), and no detectable IL-15 in the medium was verified by ELISA. 3. IL-15Ralpha was detected on the surface of HUVECs by FACS after IFN-gamma and TNF-alpha stimulation, whereas Western blotting revealed that the elevated expression on surface IL-15Ralpha was not due to the increased protein expression. The conclusion demonstrated from our results is that IFN-gamma and TNF-alpha play an important role in regulating the expression of IL-15 and IL-15Ralpha on the surface of HUVECs.
Liu
Xiuping
X
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China. Liuxiuping2006@gmail.com
Zuo
Yumei
Y
Zhang
Weina
W
Yang
Deguang
D
Xiong
Changyun
C
Zhang
Xiaozhou
X
D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology; D019409:Interleukin-15 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053647:Receptors, Interleukin-15 / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D015854:Up-Regulation
C569274:IL15 protein, human; C508024:IL15RA protein, human; D019409:Interleukin-15; D012333:RNA, Messenger; D053647:Receptors, Interleukin-15; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma