title: Inactivation of the CTD phosphatase-like gene OsCPL1 enhances the development of the abscission layer and seed shattering in rice.
abstract: Although susceptibility to seed shattering causes severe yield loss during cereal crop harvest, it is an adaptive trait for seed dispersal in wild plants. We previously identified a recessive shattering locus, sh-h, from the rice shattering mutant line Hsh that carries an enhanced abscission layer. Here, we further mapped sh-h to a 34-kb region on chromosome 7 by analyzing 240 F(2) plants and five F(3) lines from the cross between Hsh and Blue&Gundil. Hsh had a point mutation at the 3' splice site of the seventh intron within LOC_Os07g10690, causing a 15-bp deletion of its mRNA as a result of altered splicing. Two transferred DNA (T-DNA) insertion mutants and one point mutant exhibited the enhanced shattering phenotype, confirming that LOC_Os07g10690 is indeed the sh-h gene. RNA interference (RNAi) transgenic lines with suppressed expression of this gene exhibited greater shattering. This gene, which encodes a protein containing a conserved carboxy-terminal domain (CTD) phosphatase domain, was named Oryza sativa CTD phosphatase-like 1 (OsCPL1). Subcellular localization and biochemical analysis revealed that the OsCPL1 protein is a nuclear phosphatase, a common characteristic of metazoan CTD phosphatases involved in cell differentiation. These results demonstrate that OsCPL1 represses differentiation of the abscission layer during panicle development.
authors.affiliation: Department of Agricultural Bio-resources, National Academy of Agricultural Science (NAAS), Suwon 441-707, Korea.
authors.forename: Hyeonso
authors.initials: H
authors.lastname: Ji
authors.forename: Sung-Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Yul-Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Hakbum
authors.initials: H
authors.lastname: Kim
authors.forename: Moo-Young
authors.initials: MY
authors.lastname: Eun
authors.forename: Il-Doo
authors.initials: ID
authors.lastname: Jin
authors.forename: Young-Soon
authors.initials: YS
authors.lastname: Cha
authors.forename: Doh-Won
authors.initials: DW
authors.lastname: Yun
authors.forename: Byung-Ohg
authors.initials: BO
authors.lastname: Ahn
authors.forename: Myung Chul
authors.initials: MC
authors.lastname: Lee
authors.forename: Gang-Seob
authors.initials: GS
authors.lastname: Lee
authors.forename: Ung-Han
authors.initials: UH
authors.lastname: Yoon
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.forename: Seok-Cheol
authors.initials: SC
authors.lastname: Suh
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Jiang
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Jin
authors.forename: Susan R
authors.initials: SR
authors.lastname: McCouch
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
authors.forename: Hee-Jong
authors.initials: HJ
authors.lastname: Koh
mesh_terms: D000595:Amino Acid Sequence; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics / Q000254:growth & development*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D017354:Point Mutation / Q000235:genetics; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development*; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C036483:T-DNA; D049452:Green Fluorescent Proteins; D010749:Phosphoprotein Phosphatases