A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids.
Mesoporous aerogel was produced under regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer-Emmett-Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O.
Li
Yen Kuang
YK
Department of Chemistry, Chung Yuan Christian University, 200 Chung-Pei Road, Chung-Li, Taoyuan County 32023, Taiwan, ROC.
Yang
Den-Kai
DK
Chen
Yun-Chu
YC
Su
Hung-Ju
HJ
Wu
Jui-Chuang
JC
Chen-Yang
Yui Whei
YW
D015342:DNA Probes / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D015342:DNA Probes; D005782:Gels; D009711:Nucleotides; D017646:Organosilicon Compounds; D011108:Polymers; D012822:Silicon Dioxide; D009584:Nitrogen