[Development and application of a mammlian one hybrid-based high-throughput screening model for Eralpha modulator].
Estrogen Receptor (ERalpha) is a member of superfamily of ligand-activated transcription factors which play critical roles in many biological processes. To screen novel modulators of ERalpha for drug development and biological function research, we developed a mammalian one-hybrid-based high-throughput screening model for ERalpha modulator. We cloned the ERalpha LBD gene from the total mRNA of fat tissue by RT-PCR and fused it with the GAL4 DNA binding domain of pBIND-GAL4 plasmid to construct a chimara expression plasmid pBIND-GAL4-Eralpha(LBD). The L02 cells was cotransfected with pBIND-GAL4-ERalpha(LBD) and a GAL4-responsive luciferase reporter plasmid pGL3-GAL4, and following treatment with test compounds for 24 h, the activities of luciferase were detected to evaluate the transactivities of ERalpha modulators. After manner optimizations of transfection conditions, Estradiol, an agonist control, induced the expression of luciferase in a dose-dependent with EC50 of 0.17 micromol/L, the maximum folds of induction was about 28.1. Tamoxifen, an antagonist control, efficiently suppressed the estradiol-mediated luciferase induction with EC50 of 0.10 micromol/L. Using this screening model, we discovered four ERalpha agonists from 2000 natural and synthetic compounds.
Zhang
Qian
Q
College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China.
Shui
Xiaoxi
X
Fan
Yuling
Y
Hao
Weili
W
Zheng
Zhihui
Z
Lu
Xinhua
X
Zhao
Baohua
B
Zhang
Hua
H
He
Jiangong
J
D041721:3T3-L1 Cells; D000818:Animals; D002678:Chimera / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D020847:Estrogen Receptor Modulators / Q000737:chemistry / Q000302:isolation & purification*; D047628:Estrogen Receptor alpha / Q000819:agonists*; D017930:Genes, Reporter / Q000235:genetics; D019833:Genistein / Q000737:chemistry / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008956:Models, Chemical; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
D004268:DNA-Binding Proteins; D020847:Estrogen Receptor Modulators; D047628:Estrogen Receptor alpha; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D019833:Genistein; D008156:Luciferases