Meso scale discovery and luminex comparative analysis of calbindin D28K.
The sensitivity of different renal regions to xenobiotics requires the development of a multiplex immunoassay for the simultaneous analysis of kidney biomarkers. Calbindin D28K is a distal tubule-specific protein that can be detected in urine under pathological conditions. In this study, a pair of anti-calbindin D28K antibodies was used in an immunoassay for the detection of calbindin D28K expression in rat and human kidney and urine. Comparative analysis of the immunoassay was performed on the Meso Scale Development (MSD) and Luminex platforms. Analysis on both platforms detected calbindin D28K concentrations between 100 ng/mL and 100 pg/mL. Luminex detected 10-fold the amount of calbindin D28K in samples analyzed as compared to MSD, whereas calbindin D28K level in rat and human urine was below detection limit in both platforms. The application of the immunoassays described herein may be useful in toxicological and pathological studies of distal tubular damage in rats and human.
Sourial
Samer
S
Molecular Toxicology, Safety Assessment, AstraZeneca R&D Södertälje, 151 85 Södertälje, Sweden. s.sourial@student.liverpool.ac.uk
Marcusson-Ståhl
Maritha
M
Cederbrant
Karin
K
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D064092:Calbindin 1; D064026:Calbindins; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007668:Kidney / Q000378:metabolism*; D051381:Rats; D015203:Reproducibility of Results; D064030:S100 Calcium Binding Protein G / Q000737:chemistry* / Q000652:urine
D015415:Biomarkers; C576498:CALB1 protein, human; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G