title: Total blood volume is maintained in nonhydropic fetuses with severe hemolytic anemia.
abstract: OBJECTIVE
  Fetal alloimmune anemia is associated with increased blood flow velocities and cardiomegaly. In severe cases, hydrops can develop. We investigated whether the decrease of red blood cell volume is associated with a reduction or expansion of plasma volume.
  
  
  METHODS
  In 86 alloimmunized fetuses that received a first intrauterine transfusion, we calculated fetal total blood volumes (i.e. fetoplacental blood volumes) using a dilutional principle of fetal hemoglobin with adult hemoglobin. The relation between total blood volume and estimated fetal weight, severity of anemia and hydrops was analyzed.
  
  
  RESULTS
  Gestational age ranged from 17 to 35 weeks. Mean hemoglobin deficit was 6.8 standard deviations (range 2.1-11.7) below the normal mean. Fetal total blood volume was significantly related to estimated fetal weight (p < 0.001). Mean total blood volume in nonhydropic fetuses was 123 ml/kg (n = 74) and in hydropic fetuses 144 ml/kg (n = 12). There was a significant relation between total blood volume per kg body weight and hydrops (p = 0.035); however, there was no relation with severity of anemia (p = 0.94).
  
  
  CONCLUSIONS
  In the human nonhydropic fetus with severe hemolytic anemia, total blood volume is maintained: the decrease in red blood cell volume is thus compensated by an increase in plasma volume. In hydropic fetuses, however, total blood volume seems to be increased. This is in accordance with the hypothesis that congestive heart failure plays a role in the pathophysiology of hydrops in anemic fetuses.
authors.affiliation: Department of Obstetrics, Leiden University Medical Center, Leiden, The Netherlands. S.A.Pasman@LUMC.nl
authors.forename: S A
authors.initials: SA
authors.lastname: Pasman
authors.forename: C P Bil
authors.initials: CP
authors.lastname: van den Brink
authors.forename: M A
authors.initials: MA
authors.lastname: Kamping
authors.forename: P N
authors.initials: PN
authors.lastname: Adama van Scheltema
authors.forename: D
authors.initials: D
authors.lastname: Oepkes
authors.forename: F P H A
authors.initials: FP
authors.lastname: Vandenbussche
mesh_terms: D000745:Anemia, Hemolytic, Congenital / Q000097:blood / Q000175:diagnosis / Q000503:physiopathology*; D001805:Blood Transfusion, Intrauterine; D001810:Blood Volume; D005333:Fetus / Q000503:physiopathology*; D005865:Gestational Age; D006801:Humans; D015160:Hydrops Fetalis / Q000097:blood / Q000175:diagnosis / Q000503:physiopathology*; D011296:Prenatal Diagnosis / Q000379:methods