title: Acute microinstillation inhalation exposure to sarin induces changes in respiratory dynamics and functions in guinea pigs.
abstract: This study investigates the toxic effects of sarin on respiratory dynamics following microinstillation inhalation exposure in guinea pigs. Animals are exposed to sarin for 4 minutes, and respiratory functions are monitored at 4 hours and 24 hours by whole-body barometric plethysmography. Data show significant changes in respiratory dynamics and function following sarin exposure. An increase in respiratory frequency is observed at 4 hours post exposure compared with saline controls. Tidal volume and minute volume are also increased in sarin-exposed animals 4 hours after exposure. Peak inspiratory flow increases, whereas peak expiratory flow increases at 4 hours and is erratic following sarin exposure. Animals exposed to sarin show a significant decrease in expiratory time and inspiratory time. End-inspiratory pause is unchanged whereas end-expiratory pause is slightly decreased 24 hours after sarin exposure. These results indicate that inhalation exposure to sarin alters respiratory dynamics and function at 4 hours, with return to normal levels at 24 hours post exposure.
authors.affiliation: United States Army Medical Research Institute of Chemical Defense, Edgewood, MD, USA.
authors.forename: Michele L
authors.initials: ML
authors.lastname: Conti
authors.forename: Magnus M
authors.initials: MM
authors.lastname: Che
authors.forename: Megan
authors.initials: M
authors.lastname: Boylan
authors.forename: Alfred M
authors.initials: AM
authors.lastname: Sciuto
authors.forename: Richard K
authors.initials: RK
authors.lastname: Gordon
authors.forename: Madhusoodana P
authors.initials: MP
authors.lastname: Nambiar
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002619:Chemical Warfare Agents / Q000633:toxicity*; D006168:Guinea Pigs; D019570:Inhalation Exposure / Q000009:adverse effects*; D007928:Lethal Dose 50; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010100:Oxygen / Q000097:blood; D010993:Plethysmography, Whole Body; D011654:Pulmonary Edema / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D012143:Respiratory Physiological Phenomena / Q000187:drug effects*; D012524:Sarin / Q000633:toxicity*
chemical_list: D002619:Chemical Warfare Agents; D012524:Sarin; D000110:Acetylcholinesterase; D010100:Oxygen