IRF4 is modulated by CD40L and by apoptotic and anti-proliferative signals in Hodgkin lymphoma.
The effects of proliferative, apoptotic and anti-proliferative stimuli on interferon regulatory factor 4 (IRF4) expression by Reed-Sternberg (RS) cells were analysed using a panel of Hodgkin lymphoma (HL)-derived cell lines. IRF4 expressed by HL cells was consistently upregulated after CD40 engagement; IRF4 was downregulated by agonistic anti-CD95 antibodies in the FAS-sensitive HDLM-2 cells and after treatment with Adriamycin and Dacarbazine, two chemotherapic agents commonly used for HL treatment. These results demonstrated, for the first time, that IRF4 was up-modulated by CD40 engagement, and down-modulated by apoptotic and anti-proliferative signals, suggesting an involvement of IRF4 also in HL pathobiology.
Aldinucci
Donatella
D
Experimental Oncology 2, Centro di Riferimento Oncologico, IRCCS-National Cancer Institute, Aviano (PN), Italy. daldinucci@cro.it
Rapana'
Barbara
B
Olivo
Karin
K
Lorenzon
Debora
D
Gloghini
Annunziata
A
Colombatti
Alfonso
A
Carbone
Antonino
A
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D023201:CD40 Ligand / Q000276:immunology*; D049109:Cell Proliferation; D003606:Dacarbazine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000276:immunology; D006689:Hodgkin Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D050835:Interferon Regulatory Factors / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000276:immunology; D014407:Tumor Cells, Cultured; D000099218:Interferon Regulatory Factor-4
D000903:Antibiotics, Antineoplastic; D018906:Antineoplastic Agents, Alkylating; D023201:CD40 Ligand; D003606:Dacarbazine; D004317:Doxorubicin; D050835:Interferon Regulatory Factors; D000099218:Interferon Regulatory Factor-4