title: Binding of herpes simplex virus glycoprotein B (gB) to paired immunoglobulin-like type 2 receptor alpha depends on specific sialylated O-linked glycans on gB.
abstract: Paired immunoglobulin-like type 2 receptor alpha (PILRalpha) is an inhibitory receptor expressed on both hematopoietic and nonhematopoietic cells. Its binding to a cellular ligand, CD99, depends on the presence of sialylated O-linked glycans on CD99. Glycoprotein B (gB) of herpes simplex virus type 1 (HSV-1) binds to PILRalpha, and this association is involved in HSV-1 infection. Here, we found that the presence of sialylated O-glycans on gB is required for gB to associate with PILRalpha. Furthermore, we identified two threonine residues on gB that are essential for the addition of the principal O-glycans acquired by gB and that are also essential for the binding of PILRalpha to gB.
authors.affiliation: Laboratory of Immunochemistry, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Fan
authors.forename: Takeshi
authors.initials: T
authors.lastname: Satoh
authors.forename: Jun
authors.initials: J
authors.lastname: Arii
authors.forename: Lewis L
authors.initials: LL
authors.lastname: Lanier
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Spear
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Arase
mesh_terms: D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry*; D011971:Receptors, Immunologic / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; C412263:PILRA protein, human; D011134:Polysaccharides; D011971:Receptors, Immunologic; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus