title: [The study of changes on NKT cells of experimental autoimmune encephalomyelitis (EAE) mice].
abstract: OBJECTIVE
  To observe the changes of the number of NKT cells in spleens and livers of induced model of experimental autoimmune encephalomyelitis (EAE), and to study the role NKT cells play in the immunoregulation of EAE.
  
  
  METHODS
  C57BL/6 mice were immunized with MOG (35-55) peptide and received clinical evaluation daily. The mice were sacrificed at the fastigium and the splenic and hepatic lymphocytes were isolated. The changes of NKT cells in normal and EAE C57BL/6 mice were detected by flow cytometry.
  
  
  RESULTS
  The percent of NKT cells in lymphocytes of different organs of EAE model were greater decreased than in that of normal mice. The percent of NKT cells in splenic lymphocytes of normal mice was 2.22+/-0.14, while that in EAE mice was 1.94+/-0.07 (P<0.05). The percent of NKT cells in hepatic lymphocytes of normal mice was 5.52+/-2.17, while that in EAE mice was 2.67+/-1.41 (P<0.05).
  
  
  CONCLUSIONS
  The proliferation of splenic and hepatic NKT cells in C57BL/6 mice are inhibited in EAE model, which may indicate that the immune function conducted by NKT cell is down regulated in EAE mice.
authors.affiliation: Department of Stomatology Lochus 6, the Fourth Military Medical University, Xi'an 710032, China. ouyangqing-hellen@163.com
authors.forename: Qing
authors.initials: Q
authors.lastname: Ouyang
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Wei
authors.forename: Yuan-Jie
authors.initials: YJ
authors.lastname: Sun
authors.forename: Zhu-Wei
authors.initials: ZW
authors.lastname: Xu
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000166:cytology; D051379:Mice; D008969:Molecular Sequence Data; D055611:Natural Killer T-Cells / Q000166:cytology* / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: D010446:Peptide Fragments