[Biological effects of lipopolysaccharide, transforming growth factor-beta1 on murine bone marrow-derived dendritic cells].
OBJECTIVE
To explore method of stimulating murine bone marrow-derived dendritic cells by lipopolysaccharide (LPS), transforming growth factor-beta1 (TGF-beta1) and to study their biological character.


METHODS
Murine bone marrow-derived dendritic cells were cultivated with cytokine GM-CSF and IL-4 for 6 days, BMDC was stimulated by control, LPS, TGF-beta1, LPS +TGF-beta1 for 48 hours respectively.Morphological characters of BMDC were observed by a inversed microscope, surface molecules such as CD(11C), CD(80), CD(86) and MHC II were detected by flowcytometry, Interleukin-6 and interleukin-12 p70 in co-culture medium was quantified by ELISA.


RESULTS
In LPS group it presented the most typical DC morphology with the highest expression of CD(80), CD(86) and MHC II, the strongest ability in mixed lymphocyte reaction, higher level of IL-6 and IL-12 p70 compared with control, TGF-beta1, LPS+TGF-beta1 (P<0.05). While in TGF-beta1 group it presented the less typical DC morphology with the lower expression of CD(80), CD(86), MHC II, weaker ability in mixed lymphocyte reaction, and lower levels of IL-6 and IL-12 p70 compared with control and LPS (P<0.05).


CONCLUSIONS
LPS can stimulate maturation of BMDC in its late differentiation which makes it presents a more significant biological characteristics.TGF-beta1 can inhibit maturation but not differentiation of BMDC thereby can prevent its biological characteristic presentation.
Feng
Lin-Lin
LL
Center of Respiratory Diseases, Children's Hospital, Chongqing Medical University, Chongqing 400014, China. fenglinlin112358@163.com
Dai
Ji-Hong
JH
Fu
Zhou
Z
Liu
Zheng
Z
Wang
Li-Jia
LJ
Li
Xin
X
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000276:immunology; D018664:Interleukin-12 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053773:Transforming Growth Factor beta1 / Q000276:immunology*
D015703:Antigens, CD; D015415:Biomarkers; D015850:Interleukin-6; D008070:Lipopolysaccharides; D053773:Transforming Growth Factor beta1; D018664:Interleukin-12