title: Fontibacter flavus gen. nov., sp. nov., a member of the family 'Cyclobacteriaceae', isolated from a hot spring.
abstract: The taxonomic position of a bright orange-pigmented bacterial strain, designated CC-GZM-130(T), isolated from a water sample of the Guan-zing-ling hot spring, southern Taiwan, was studied. The strain was able to grow on nutrient agar at 25-40 degrees C and in the presence of 1-3 % (w/v) NaCl. Comparative analyses of the 16S rRNA gene sequence showed that the isolate was grouped in the vicinity of the genus Aquiflexum with the highest sequence similarity of 92.1 % to the type strain of Aquiflexum balticum, followed by sequence similarities of 92.0, 91.6 and 91.5 % to the type strains of Algoriphagus ornithinivorans, Algoriphagus hitonicola and Belliella baltica, respectively. The polyamine pattern showed that the major compound was sym-homospermidine. The quinone system was menaquinone MK-7. The polar lipid profile was composed predominantly of phosphatidylethanolamine, three polar lipids and one aminolipid. Minor amounts of other lipids were also detectable. The main characteristics of the fatty acid profiles of strain CC-GZM-130(T), B. baltica and Aquiflexum balticum were similar, with iso-C(15 : 0), iso-C(17 : 1)ω 9c and iso-C(17 : 0) 3-OH as the major fatty acids, but some qualitative and quantitative differences were observed. The DNA G+C content of the novel strain was 53.2 mol%. The isolate clearly differed genotypically and phenotypically from representatives of the most closely related genera. On the basis of these differences, a novel species in a new genus, Fontibacter flavus gen. nov., sp. nov., is proposed with CC-GZM-130(T) (=CCUG 57694(T)=CCM 7650(T)) as the type strain of the type species.
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, IFZ - Heinrich-Buff-Ring 26-32, D35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Yenepoya Research Center, Yenepoya University, Nityanada Nagar, Deralakatte, Mangalore 575 018, Karnataka, India.
authors.forename: P D
authors.initials: PD
authors.lastname: Rekha
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, IFZ - Heinrich-Buff-Ring 26-32, D35392 Giessen, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Fallschissel
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, IFZ - Heinrich-Buff-Ring 26-32, D35392 Giessen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Lodders
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Jui-Hsing
authors.initials: JH
authors.lastname: Chou
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Fo-Ting
authors.initials: FT
authors.lastname: Shen
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Wien, Austria.
authors.forename: Alexa
authors.initials: A
authors.lastname: Frischmann
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Yenepoya Research Center, Yenepoya University, Nityanada Nagar, Deralakatte, Mangalore 575 018, Karnataka, India.
authors.forename: A B
authors.initials: AB
authors.lastname: Arun
mesh_terms: D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S