[Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in somatic cytochrome C gene].
Mouse embryonic fibroblasts (MEF) with point mutation in somatic cytochrome C gene were generated and characterized. It was shown that substitution of lysine for tryptophan in position 72 (K72W) decreased the proapoptotic functions of cytochrome C in response to staurosporin treatment without disrupting its respiratory functions. The presence of this mutation did not affect the pattern of cytochrome C gene expression or its localization inside the cell. These cell cultures therefore represent an interesting model for study apoptotic signaling and physiological functions of cytochrome C.
Mufazalov
I A
IA
Pen'kov
D N
DN
Cherniak
B V
BV
Pletiushkina
O Iu
OIu
Vysokikh
M Iu
MIu
Kirpichnikov
M P
MP
Dolgikh
D A
DA
Kruglov
A A
AA
Kuprash
D V
DV
Skulachev
V P
VP
Nedospasov
S A
SA
D019943:Amino Acid Substitution; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002460:Cell Line; D045304:Cytochromes c / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D008954:Models, Biological; D020125:Mutation, Missense; D019311:Staurosporine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D045304:Cytochromes c; D019311:Staurosporine