title: Improved production of homo-D-lactic acid via xylose fermentation by introduction of xylose assimilation genes and redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-Lactate dehydrogenase gene-deficient Lactobacillus plantarum.
abstract: The production of optically pure d-lactic acid via xylose fermentation was achieved by using a Lactobacillus plantarum NCIMB 8826 strain whose l-lactate dehydrogenase gene was deficient and whose phosphoketolase genes were replaced with a heterologous transketolase gene. After 60 h of fermentation, 41.2 g/liter of d-lactic acid was produced from 50 g/liter of xylose.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Okano
authors.forename: Shogo
authors.initials: S
authors.lastname: Yoshida
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Yamada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D005285:Fermentation; D007218:Industrial Microbiology; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis* / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D014994:Xylose / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D014994:Xylose; D007770:L-Lactate Dehydrogenase