Characterization of molds isolated from smoked paprika by PCR-RFLP and micellar electrokinetic capillary electrophoresis.
Molds are common contaminants of paprika meat products. The drying and storage stages of paprika processing are critical because they can provide molds with the conditions particularly appropriate for their growth and proliferation. Thus, an efficient and accurate characterization of the toxigenic molds of paprika is necessary. An RFLP analysis of the rRNA genes was performed by using a TaqI restriction enzyme. In addition, a micellar electrokinetic capillary chromatography (MECC) method was tested to analyze secondary metabolites produced by mold strains commonly found in paprika. This study was confirmed with a 5.8S-ITS region sequence analysis. A total of 31 isolates were identified by RFLP and MECC analysis. These showed stable RFLP profiles that were clearly different for the different genera and species, and were grouped into clusters together with the profiles of the 16 reference strains. MECC analysis provided additional characteristic peak patterns for the characterization of the mold species present. The characterized isolates were species of the genera Fusarium spp., Aspergillus spp., Penicillium spp., Cladosporium spp., Mucor spp. and Phlebia spp. The identifications were confirmed by the 5.8S-ITS region sequence analysis and by a BLAST search of the GenBank database. RFLP patterns with TaqI restriction enzyme and MECC profiles, either singly or combined, could be of great interest to distinguish molds in paprika.
Ruiz-Moyano
Santiago
S
Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, 06071 Badajoz, Spain.
Benito
María J
MJ
Martín
Alberto
A
Aranda
Emilio
E
Hernández
Alejandro
A
Córdoba
María G
MG
D002212:Capsicum / Q000382:microbiology*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005658:Fungi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D008970:Molecular Weight; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D004271:DNA, Fungal