title: Characterization for binding complex formation with site-directly immobilized antibodies enhancing detection capability of cardiac troponin I.
abstract: The enhanced analytical performances of immunoassays that employed site-directly immobilized antibodies as the capture binders have been functionally characterized in terms of antigen-antibody complex formation on solid surfaces. Three antibody species specific to cardiac troponin I, immunoglobulin G (IgG), Fab, and F(ab')(2) were site-directly biotinylated within the hinge region and then immobilized via a streptavidin-biotin linkage. The new binders were more efficient capture antibodies in the immunoassays compared to randomly bound IgG, particularly, in the low antibody density range. The observed improvements could have resulted from controlled molecular orientation and also from flexibility, offering conditions suitable for binding complex formations.
authors.affiliation: Program for Bio-Microsystem Technology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-ku, Seoul 136-701, South Korea.
authors.forename: Il-Hoon
authors.initials: IH
authors.lastname: Cho
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Seo
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Jeon
authors.forename: Se-Hwan
authors.initials: SH
authors.lastname: Paek
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000915:Antibody Affinity; D001710:Biotin / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D007700:Kinetics; D016014:Linear Models; D011485:Protein Binding; D019210:Troponin I / Q000032:analysis* / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D019210:Troponin I; D001710:Biotin