title: Expression of the Arabidopsis thaliana histone gene AtHTA1 enhances rice transformation efficiency.
abstract: We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
authors.forename: Y E
authors.initials: YE
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: He
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Ying
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China.
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Lu
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
authors.forename: Stanton B
authors.initials: SB
authors.lastname: Gelvin
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China. Electronic address: huixia@zju.edu.cn.
authors.forename: Hui-Xia
authors.initials: HX
authors.lastname: Shou
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins