title: [Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization].
abstract: OBJECTIVE
  To study the relationship between the inhibitory effects of Tongxie Yaofang, a compound traditional Chinese herbal medicine, on the contraction of the colonic smooth muscle isolated from rats and calcium mobilization.
  
  
  METHODS
  By measuring the tension of the isolated colonic smooth muscle strips, the inhibitory effects of Tongxie Yaofang on the contraction induced by acetylcholine (ACh), KCl and exhausting Ca(2+) of internal calcium store were assessed respectively.
  
  
  RESULTS
  Tongxie Yaofang could concentration-dependently inhibit the contraction of isolated rat colonic smooth muscle strips induced by KCl and exhausting the Ca(2+) of internal calcium store. Tongxie Yaofang could also inhibit the tension of the second contractile phase induced by ACh (P<0.01, vs control), but had no influence on the first contractile phase.
  
  
  CONCLUSIONS
  Tongxie Yaofang can inhibit the contraction of isolated rat colonic smooth muscle strips mainly by preventing the influx of extracellular Ca(2+), which may be associated with blocking voltage-dependent channel, store-operated channel and receptor-operated channel, but not by preventing the release of internal Ca(2+) from calcium store.
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Jian-ye
authors.initials: JY
authors.lastname: Yuan
authors.forename: Jian-qun
authors.initials: JQ
authors.lastname: Xie
authors.forename: Da-zheng
authors.initials: DZ
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiang-xue
authors.initials: XX
authors.lastname: Pan
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Fei
authors.forename: Hai-zhen
authors.initials: HZ
authors.lastname: Xu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D002118:Calcium