title: LuMPIS--a modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs.
abstract: The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems.
authors.affiliation: Max von Pettenkofer-Institute, Ludwig-Maximilians University, Munich, Germany.
authors.forename: Maria Guadalupe
authors.initials: MG
authors.lastname: Vizoso Pinto
authors.forename: Josefina Maria
authors.initials: JM
authors.lastname: Villegas
authors.forename: Jan
authors.initials: J
authors.lastname: Peter
authors.forename: Rudolf
authors.initials: R
authors.lastname: Haase
authors.forename: Jürgen
authors.initials: J
authors.lastname: Haas
authors.forename: Amelie Sophia
authors.initials: AS
authors.lastname: Lotz
authors.forename: Ania Carolina
authors.initials: AC
authors.lastname: Muntau
authors.forename: Armin
authors.initials: A
authors.lastname: Baiker
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D020862:GC Rich Sequence; D015967:Gene Expression Regulation, Viral; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D058619:Maltose-Binding Proteins; D016366:Open Reading Frames; D025941:Protein Interaction Mapping / Q000191:economics / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D049490:Systems Biology / Q000379:methods; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D014764:Viral Proteins