title: The signal/noise of an HMBC spectrum can depend dramatically upon the choice of acquisition and processing parameters.
abstract: The effect of various acquisition and processing parameters on the sensitivity of HMBC spectra for typical organic molecules has been systematically investigated. For molecules in the 200-600 molecular weight range, an acquisition time of 0.2 to 0.4 s, a recycle time of no more than 1.0 s, optimization for (n)J(CH) = 8 Hz and 512 time increments (with two- to fourfold linear prediction) are recommended. Some form of sine bell weighting along f2 and either Gaussian or sine bell weighting along f1 is suggested. The use of a 0.1-s acquisition time and/or Gaussian or exponential weighting along f2 can result in dramatic sensitivity loss, particularly for correlation peaks involving protons with complex splitting patterns, and should be avoided.
authors.affiliation: Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada.
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Burrow
authors.forename: Raul G
authors.initials: RG
authors.lastname: Enriquez
authors.forename: William F
authors.initials: WF
authors.lastname: Reynolds