[Anti-proliferation action of taurine on rat cardiac fibroblast through inhibiting protein kinase Calpha expression].
This project aimed to investigate the effect of taurine on cell cycle regulatory protein p27, Cyclin D1 and nuclear factor-kappa B (NF-kappaB) p65 in the proliferation of cultured neonatal rat cardiac fibroblast (CFb) induced by angiotensin II (Ang II), and to explore the effect of taurine on the signal transduction pathway in CFb proliferation. The cultured neonatal rat CFbs were isolated by trypsin digestion method. The proliferation of CFb was induced by Ang II and detected with thiazole blue (MTT) colorimetric assay. The protein expression of p-PKCalpha in cells was determined with Western blotting technology. The expression of p27 was analyzed by flow cytometry. The expression of Cyclin D1 was determined with the combination of immunocytochemical staining and image analysis software. The nuclear translocation of NF-kappaB p65 was determined with immunofluorescence staining. Among the concentrations ranged from 40 to 160 mmol L(-1), taurine significantly inhibited p-PKCalpha expression. Taurine increased p27 expression and inhibited the nuclear translocation of NF-kappaB p65 in CFb (P < 0.05, P < 0.01, respectively) by inhibition of p-PKCalpha expression. And PKC inhibitor (Che) could improve the inhibitory action of taurine on CFb proliferation. The effects of taurine on CFb proliferation might be due to inhibition of p-PKCalpha expression and p27 expression increase and the nuclear translocation of NF-kappaB p65 inhibition followed.
Wang
Yan-Chun
YC
School of Basic Medicine, Jilin Medical College, Jilin 132013, China.
Guan
Feng-Ying
FY
Li
Hong
H
Yang
Shi-Jie
SJ
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013654:Taurine / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000378:metabolism
C530106:Ccnd1 protein, rat; C493962:Cdkn1b protein, rat; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D013654:Taurine; D051571:Protein Kinase C-alpha