Formation of new blood vessels in the heart can be studied in cell cultures.
Controlled induction of the formation of new microvessels, i.e., therapeutic angiogenesis, may be used one day to treat patients that for example had suffered a myocardial infarction. Experimental models of angiogenesis in the heart in vivo substantially stress the animal. We therefore developed a model of angiogenesis of the heart in vitro, where mouse and rat heart pieces are stimulated under controlled conditions in a three dimensional matrix. Capillary-like sprouts emerging in these cultures represent early to midterm steps of angiogenesis and can be quantified to study potential angiogenic compounds and underlying mechanisms.
Humar
Rok
R
Department of Research, University Hospital Basel, Basel, Switzerland. rok.humar@unibas.ch
de Miguel
Lourdes Sanchez
LS
Kiefer
Fabrice N
FN
Battegay
Edouard J
EJ
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D000077214:Becaplermin; D002196:Capillaries / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005337:Fibrin / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005782:Gels; D006321:Heart / Q000502:physiology*; D006801:Humans; D051379:Mice; D009129:Muscle Tonus / Q000187:drug effects / Q000502:physiology; D009206:Myocardium / Q000166:cytology / Q000201:enzymology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009569:Nitric Oxide / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000172:deficiency; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D014664:Vasodilation / Q000187:drug effects
D043925:Angiogenesis Inducing Agents; D005782:Gels; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D016222:Fibroblast Growth Factor 2; D000804:Angiotensin II; D000077214:Becaplermin; D009569:Nitric Oxide; D005337:Fibrin; D052247:Nitric Oxide Synthase Type II