Herpetomonas trimorpha sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera).
Monoxenous trypanosomatid Herpetomonas trimorpha sp. nov. was isolated from the digestive tract of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera). This species forms three distinct morphotypes in culture: the microflagellate promastigote, the small promastigote and the long promastigote. The last form is unique for the newly described species. Phylogenetic analyses of SSU rRNA and glycosomal glyceraldehyde phosphate dehydrogenase genes showed that H. trimorpha sp. nov. is the closest relative of Herpetomonas ztiplika, another monoxenous trypanosomatid isolated from biting midges. However, morphological and randomly amplified polymorphic DNA analyses confirmed that H. trimorpha sp. nov. is distinct from H. ztiplika.
Zídková
Lenka
L
Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
Cepicka
Ivan
I
Department of Zoology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
Votýpka
Jan
J
Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
Svobodová
Milena
M
Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 128 44, Czech Republic.
D000818:Animals; D002519:Ceratopogonidae / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D041981:Gastrointestinal Tract / Q000469:parasitology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D014351:Trypanosomatina / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D015800:Protozoan Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases