title: Molecular characterisation of PFGE non-typable methicillin-resistant Staphylococcus aureus in The Netherlands, 2007.
abstract: In 2007 in The Netherlands, 30% of all human isolates of methicillin-resistant Staphylococcus aureus (MRSA) sent to the National Institute for Public Health and the Environment could not be typed by pulsed-field gel electrophoresis (non-typable (NT)-MRSA). Molecular characterisation of the NT-MRSA isolates revealed 27 different spa types and two distinct SCCmec types, type IV and V. All NT-MRSA isolates were closely related based on spa and multi-locus sequence typing and belonged to the ST398 lineage. The rapid increase of NT-MRSA (ST398) isolates over the last years shows the importance of this relatively new clonal lineage.
authors.affiliation: National Institute for Public Health and the Environment, Bilthoven, The Netherlands. Xander.Huijsdens@rivm.nl
authors.forename: X W
authors.initials: XW
authors.lastname: Huijsdens
authors.forename: T
authors.initials: T
authors.lastname: Bosch
authors.forename: M G
authors.initials: MG
authors.lastname: van Santen-Verheuvel
authors.forename: E
authors.initials: E
authors.lastname: Spalburg
authors.forename: G N
authors.initials: GN
authors.lastname: Pluister
authors.forename: M
authors.initials: M
authors.lastname: van Luit
authors.forename: M E O C
authors.initials: ME
authors.lastname: Heck
authors.forename: A
authors.initials: A
authors.lastname: Haenen
authors.forename: A J
authors.initials: AJ
authors.lastname: de Neeling
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000302:isolation & purification*; D015336:Molecular Probe Techniques; D009426:Netherlands