Simultaneous evaluation of toxicities using a mammalian cell array chip prepared by photocatalytic lithography.
A prototype of a mammalian cell array chip was developed on a flat glass surface. A superhydrophilic (water contact angle=5 degrees)/highly hydrophobic (120 degrees) pattern was prepared on a fluorinated polymer-coated glass surface by means of photocatalytic lithography, and A549 (a human alveolar epithelial cell line), Hep G2 (a human hepatoma cell line) and mouse fibroblast 3T3 cells were inoculated onto the superhydrophilic regions. The cell populations were confined in the superhydrophilic regions for at least 24 h and separated from each other for at least one week. Organ-specific toxicity of aflatoxin B(1) and non-specific toxicity of adriamycin were successfully detected by using the cell array chip.
Komori
Kikuo
K
Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. kkomori@iis.u-tokyo.ac.jp
Nada
Jun
J
Nishikawa
Masaki
M
Notsu
Hideo
H
Tatsuma
Tetsu
T
Sakai
Yasuyuki
Y
D016604:Aflatoxin B1 / Q000032:analysis / Q000633:toxicity; D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000633:toxicity; D002384:Catalysis; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000032:analysis / Q000633:toxicity; D004354:Drug Screening Assays, Antitumor; D005898:Glass / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D041681:NIH 3T3 Cells; D010777:Photochemistry; D013499:Surface Properties; D046888:Tissue Array Analysis / Q000295:instrumentation* / Q000379:methods*; D018675:Toxicity Tests / Q000295:instrumentation / Q000379:methods*
D000970:Antineoplastic Agents; D004317:Doxorubicin; D016604:Aflatoxin B1