title: Mustard oil in "Shibori Daikon" a variety of Japanese radish, selectively inhibits the proliferation of H-ras-transformed 3Y1 cells.
abstract: Cruciferous vegetables and their isothiocyanates are promising foods and agents for cancer prevention. We focus here on the effects of mustard oil (SMO) in a variety of the Japanese radish, Shibori Daikon (Raphanus sativus), on the proliferation of 3Y1 rat fibroblasts and the H-ras-transformed derivative, HR-3Y1-2. SMO (1.6 microg/ml) inhibited the proliferation of HR-3Y1-2, but not 3Y1 after 24 h after treatment. A cell cycle analysis showed that SMO induced G2/M arrest after 6 h, although this effect was not observed 24 h after the treatment. SMO transiently decreased the cellular reduced glutathione level accompanied with up-regulation of the intracellular reactive oxygen species 2-3 h post-treatment. Glutathione ethyl ester and N-acetyl-L-cysteine prevented the growth inhibitory effect of SMO. This mustard oil extract consisted of 95.6% 4-methylthio-3-butenyl isothiocyanate and 4.4% 4-methylthiobutyl isothiocyanate. SMO selectively inhibited H-ras-transformed 3Y1 cells associated with transient oxidative stress via reduced glutathione (GSH) depletion.
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Omi
authors.forename: Naoko
authors.initials: N
authors.lastname: Fujii
authors.forename: Asako
authors.initials: A
authors.lastname: Ozaki
authors.forename: Akihiko
authors.initials: A
authors.lastname: Nakama
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D005978:Glutathione / Q000378:metabolism; D042541:Intracellular Space / Q000235:genetics / Q000378:metabolism; D009149:Mustard Plant; D010938:Plant Oils / Q000494:pharmacology*; D031224:Raphanus / Q000737:chemistry*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D010938:Plant Oils; D017382:Reactive Oxygen Species; D018631:ras Proteins; D005978:Glutathione; C027793:mustard oil