Kinetic approach for evaluation of total antioxidant activity.
We propose a novel approach for assessment of total antioxidant activity by monitoring kinetics of hydrogen peroxide (H(2)O(2)) scavenging after its injection into liquid sample under study. H(2)O(2) is known to be the strongest oxidant, really presented in human body in contrast to the majority of the model oxidative systems used for evaluation of antioxidant activity. In addition, kinetic approach, being more informative than the commonly used determination of the final product, obviously provides better discrimination of potential antioxidants. Prussian Blue based sensor due to its high sensitivity and operational stability allowed to monitor kinetics of hydrogen peroxide consumption in turbid and colored samples. The pseudo-first order kinetic constants of hydrogen peroxide scavenging in the presence of different food additives correlated with total antioxidant activity of these samples evaluated via standard procedure based on lipid peroxidation. However, in contrast to the standard method, the proposed kinetic approach is expressed and does not require fresh biological tissues.
Karyakina
Elena E
EE
Chemistry Faculty of M.V. Lomonosov Moscow State University, Moscow, Russia.
Vokhmyanina
Darya V
DV
Sizova
Natalya V
NV
Sabitov
Aytugan N
AN
Borisova
Anastasiya V
AV
Sazontova
Tatyana G
TG
Arkhipenko
Yury V
YV
Tkachuk
Vsevolod A
VA
Zolotov
Yury A
YA
Karyakin
Arkady A
AA
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D001628:Beverages / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D032083:Citrus paradisi; D032084:Citrus sinensis; D004563:Electrochemistry; D005295:Ferrocyanides / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D015227:Lipid Peroxidation; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D013053:Spectrophotometry; D014920:Wine / Q000032:analysis
D000975:Antioxidants; D005295:Ferrocyanides; D008563:Membrane Lipids; D006861:Hydrogen Peroxide; C000170:ferric ferrocyanide