Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene.
Growth-promoting cytokines induce expression of nuclear proto-oncogenes that play critical roles in the regulation of cell proliferation. c-myc gene is one of those nuclear proto-oncogenes, whose regulation mechanisms of cytokine-mediated expression are not fully understood. Here, I generated a green fluorescent protein (GFP) reporter system that faithfully reflects interleukin-3 (IL-3)-induced c-myc gene expression. Flowcytometric analysis revealed cytokine-specific expression of reporter GFP. Kinetics of GFP mRNA expression was similar to that of endogenous c-myc mRNA. The reporter cell line will be a useful tool for studies of cell proliferation regulation through analysis of cytokine-induced c-myc gene expression.
Fujii
Hodaka
H
Department of Pathology, New York University School of Medicine, 550 First Avenue, MSB-126, New York, NY 10016, USA. hodaka@med.nyu.edu
D001681:Biological Assay / Q000379:methods*; D002460:Cell Line / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D017930:Genes, Reporter / Q000235:genetics*; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D016207:Cytokines; C489428:Myc protein, mouse; D016271:Proto-Oncogene Proteins c-myc