[Differences of acetylcholinesterase level in variety classes and strains of Culex pipiens pallens].
To investigate the diversity of acetylcholinesterase (AChE) activity in variety classes and strains of Culex pipiens pallens and provide a basis for the insecticide-resistance detection of mosquito by biochemical method.



AChE insensitivity of single mosquito was determined, using acetythiocholine iodide (ATch) as the substrate, 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB) as the developer, and propoxur as the inhibitor.



There were significant differences in AChE activity among the four types of IV instar larvae and 3-day-old adult female of sensitive strain mosquito (P<0.01). The AChE activity of the 3-day-old adult female was higher than that of IV instar larvae of the four types of sensitive strain mosquito (P<0.01). The AChE activity of anti-DDVP (Rd) and anti-propoxur (Rp) strains of Culex pipiens pallens was significantly higher than that of sensitive (S) strain (P<0.01), while the AChE activity of anti-cypermethrin (Rc) strain of Culex pipiens pallens was similar to that of S strain (P>0.05). The individual frequency of insensitive AChE of Rd and Rp strains of Culex pipiens pallens was significantly higher than that of sensitive (S) strain (P<0.01), while the individual frequency of insensitive AChE of Rc strain of Culex pipiens pallens was similar to that of S strain(P>0.05).



The AChE activity determination can be used to examine the insecticide-resistance of mosquito.
Li
Shi-Gen
SG
Department of Parasitology, Jining Medical College, Jining 272013, China. shigenli@yahoo.com.cn
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D003465:Culex / Q000145:classification* / Q000201:enzymology*; D004006:Dichlorvos / Q000494:pharmacology; D005260:Female; D007305:Insecticide Resistance; D001074:Propoxur / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D013045:Species Specificity
D011722:Pyrethrins; C017160:cypermethrin; D004006:Dichlorvos; D001074:Propoxur; D000110:Acetylcholinesterase