title: PriA participates in nascent DNA synthesis in Escherichia coli.
abstract: The question of whether discontinuous DNA replication operates only for the lagging strand or for both strands in E. coli remains unresolved. In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig(+) polA(+) strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of nascent DNA.
authors.affiliation: Department of Molecular and Cellular Biology, Chang Gung University, Kwei-San, Tao-Yuan, 333, Taiwan.
authors.forename: Yen-Yu
authors.initials: YY
authors.lastname: Chen
authors.forename: Helen
authors.initials: H
authors.lastname: Huang
authors.forename: Tzu-Chien V
authors.initials: TC
authors.lastname: Wang
mesh_terms: D004265:DNA Helicases / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C506743:priA protein, E coli; D004265:DNA Helicases