Radioprotection by short-term oxidative preconditioning: role of manganese superoxide dismutase.
Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H(2)O(2) increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD.
Belikova
Natalia A
NA
Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Glumac
Ashley
A
Rafikov
Ruslan
R
Jiang
Jianfei
J
Greenberger
Joel S
JS
Kagan
Valerian E
VE
Bayir
Hülya
H
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors; D004622:Embryo, Mammalian / Q000166:cytology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
D017382:Reactive Oxygen Species; D012402:Rotenone; D000968:Antimycin A; D013482:Superoxide Dismutase; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III