title: Local secretion of urocortin 1 promotes microvascular permeability during lipopolysaccharide-induced inflammation.
abstract: Urocortin 1 (Ucn1) is a neuropeptide that regulates vascular tone and is implicated in both the vascular and immune cell-mediated responses to inflammation. The role of Ucn1 in regulating microvascular permeability has not been determined. We hypothesized that local Ucn1 release promotes microvascular permeability and that this effect augments the local gastrointestinal vascular response to lipopolysaccharide (LPS)-induced systemic inflammation. We measured hydraulic (L(p)) and macromolecule permeability in mesenteric venules. We show that a continuous infusion of 10(-7) m Ucn1 in a postcapillary venule increased L(p) 2-fold over baseline, as did LPS-induced inflammation. However, simultaneous infusion of Ucn1 and LPS markedly increased L(p) by 7-fold. After local knockdown of Ucn1 using RNA interference, infusion of Ucn1 with LPS resulted in return to 2-fold increase, confirming that Ucn1 synergistically augments hydraulic permeability during inflammation. LPS and Ucn1 treatment also resulted in increased numbers of interstitial microspheres, which colocalized with CD31(+) immune cells. Ucn1 activity is mediated through two receptor subtypes, CRH-R(1) and CRH-R(2). CRH-R(1) receptor blockade exacerbated, whereas CRH-R(2) receptor blockade decreased the LPS-induced increase in L(p). Finally, treatment with the c-JUN N-terminal kinase (JNK) antagonist SP600125 during infusion of LPS, but not Ucn1, decreased L(p). These findings suggest that Ucn1 increases microvascular permeability and acts synergistically with LPS to increase fluid and macromolecule losses during inflammation. Knockdown of endogenous Ucn1 during inflammation attenuates synergistic increases in L(p). Ucn1's effect on L(p) is partially mediated by the CRH-R(2) receptor and acts independently of the c-JUN N-terminal kinase signal transduction pathway.
authors.affiliation: Department of Surgery, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Cureton
authors.forename: Alexander Q
authors.initials: AQ
authors.lastname: Ereso
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Victorino
authors.forename: Brian
authors.initials: B
authors.lastname: Curran
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Poole
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Alden H
authors.initials: AH
authors.lastname: Harken
authors.forename: Aditi
authors.initials: A
authors.lastname: Bhargava
mesh_terms: D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects / Q000502:physiology*; D004357:Drug Synergism; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008643:Mesentery / Q000098:blood supply*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011743:Pyrimidines / Q000494:pharmacology; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D018019:Receptors, Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D054832:Urocortins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000099052:CRF Receptor, Type 1
chemical_list: C471210:2-methyl-4-(N-propyl-N-cycloproanemethylamino)-5-chloro-6-(2,4,6-trichloranilino)pyrimidine; D000814:Aniline Compounds; D000873:Anthracenes; D015850:Interleukin-6; D008070:Lipopolysaccharides; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011743:Pyrimidines; D018019:Receptors, Corticotropin-Releasing Hormone; D014409:Tumor Necrosis Factor-alpha; D054832:Urocortins; C432165:pyrazolanthrone; D000099052:CRF Receptor, Type 1; D048031:JNK Mitogen-Activated Protein Kinases