[The progress of research on the influencing factor of nuclear transfer].
Nuclear transfer (NT) is a new cloning technology developed in recent years. NT methods consist of electrofusion, NT mediated by polyethylene glycol (PEG) and microinjection. The success of somatic nuclear transfer depends on the source of donor nucleus, developmental stage of recipient cytoplasts, cell cycle synchrony of donor nucleus. Different methods of harvesting cells have effect on the efficiency of NT. The somatic nucleus will be reprogrammed after NT and will restore a totipotent state in order to undergo development.
Zhou
Zhujuan
Z
Department of Neurology, Xingiao Hospital, Third Military Medical University, Chongqing 400037, China.
Zheng
Jian
J
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D019976:Cloning, Organism; D004624:Embryo Transfer; D006801:Humans; D008845:Microinjections; D053652:Nuclear Transfer Techniques / Q000639:trends*; D009865:Oocytes / Q000166:cytology / Q000502:physiology*