title: Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.
abstract: A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.
authors.affiliation: Department of Biological Science, Cork Institute of Technology, Cork, Ireland.
authors.forename: James
authors.initials: J
authors.lastname: Carroll
authors.forename: Pierre
authors.initials: P
authors.lastname: Douarre
authors.forename: Aidan
authors.initials: A
authors.lastname: Coffey
authors.forename: Jim
authors.initials: J
authors.lastname: Buckley
authors.forename: Bill
authors.initials: B
authors.lastname: Cashman
authors.forename: Kevin
authors.initials: K
authors.lastname: O'Farrell
authors.forename: Jim
authors.initials: J
authors.lastname: O'Mahony
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D009162:Mycobacterium avium / Q000166:cytology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology