title: Effects of cyclic flexure on endothelial permeability and apoptosis in arterial segments perfused ex vivo.
abstract: Certain arteries (e.g., coronary, femoral, etc.) are exposed to cyclic flexure due to their tethering to surrounding tissue beds. It is believed that such stimuli result in a spatially variable biomechanical stress distribution, which has been implicated as a key modulator of remodeling associated with atherosclerotic lesion localization. In this study we utilized a combined ex vivo experimental/computational methodology to address the hypothesis that local variations in shear and mural stress associated with cyclic flexure influence the distribution of early markers of atherogenesis. Bilateral porcine femoral arteries were surgically harvested and perfused ex vivo under pulsatile arterial conditions. One of the paired vessels was exposed to cyclic flexure (0-0.7 cm(-1)) at 1 Hz for 12 h. During the last hour, the perfusate was supplemented with Evan's blue dye-labeled albumin. A custom tissue processing protocol was used to determine the spatial distribution of endothelial permeability, apoptosis, and proliferation. Finite element and computational fluid dynamics techniques were used to determine the mural and shear stress distributions, respectively, for each perfused segment. Biological data obtained experimentally and mechanical stress data estimated computationally were combined in an experiment-specific manner using multiple linear regression analyses. Arterial segments exposed to cyclic flexure had significant increases in intimal and medial apoptosis (3.42+/-1.02 fold, p=0.029) with concomitant increases in permeability (1.14+/-0.04 fold, p=0.026). Regression analyses revealed specific mural stress measures including circumferential stress at systole, and longitudinal pulse stress were quantitatively correlated with the distribution of permeability and apoptosis. The results demonstrated that local variation in mechanical stress in arterial segments subjected to cyclic flexure indeed influence the extent and spatial distribution of the early atherogenic markers. In addition, the importance of including mural stresses in the investigation of vascular mechanopathobiology was highlighted. Specific example results were used to describe a potential mechanism by which systemic risk factors can lead to a heterogeneous disease.
authors.affiliation: Department of Surgery, McGowan Institute for Regenerative Medicine, Center for Vascular Remodeling and Regeneration, University of Pittsburgh, PA 15219, USA.
authors.forename: J Scott
authors.initials: JS
authors.lastname: Van Epps
authors.forename: Douglas W
authors.initials: DW
authors.lastname: Chew
authors.forename: David A
authors.initials: DA
authors.lastname: Vorp
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D050197:Atherosclerosis; D015415:Biomarkers; D006690:Bisbenzimidazole / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005070:Evans Blue / Q000378:metabolism; D005263:Femoral Artery / Q000502:physiology*; D020342:Finite Element Analysis; D019085:Fluorescent Antibody Technique, Direct; D005456:Fluorescent Dyes / Q000378:metabolism; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D016014:Linear Models; D010477:Perfusion / Q000379:methods; D010539:Permeability; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011673:Pulsatile Flow; D033081:Shear Strength; D013314:Stress, Mechanical; D013552:Swine; D017539:Tunica Intima / Q000166:cytology / Q000502:physiology; D017540:Tunica Media / Q000166:cytology / Q000502:physiology
chemical_list: D000418:Albumins; D015415:Biomarkers; D004396:Coloring Agents; D005456:Fluorescent Dyes; D018809:Proliferating Cell Nuclear Antigen; D005070:Evans Blue; D006690:Bisbenzimidazole