title: Seed-conjugated polymer bead for beta2-microglobulin removal at neutral pH.
abstract: beta2-Microglobulin (beta2m) is known to be a major factor for dialysis-related amyloidosis. We have studied beta2m removal through an aggregation process, which was initiated by a ligand that could catch the beta2m monomer and promote its aggregation into fibril. As a ligand, we have prepared beta2m fibril fragments and used them as a seed. The seed was coupled to PEGylated-PS beads to remove the monomeric beta2m from solution. The beta2m seed-conjugated resin effectively adsorbed the beta2m monomers with a capacity of 3.6 mg/ml via promoting their aggregation into fibrils on the resin at pH 7.4.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea.
authors.forename: Mira
authors.initials: M
authors.lastname: Kim
authors.forename: Sungsoo
authors.initials: S
authors.lastname: Kang
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Myung
authors.forename: Minkoo
authors.initials: M
authors.lastname: Ahn
authors.forename: Jeong-Hyun
authors.initials: JH
authors.lastname: Choi
authors.forename: Seung R
authors.initials: SR
authors.lastname: Paik
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D006801:Humans; D006863:Hydrogen-Ion Concentration; D007676:Kidney Failure, Chronic / Q000378:metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D011487:Protein Conformation; D012639:Seeds / Q000737:chemistry*; D012996:Solutions; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D012996:Solutions; D001613:beta 2-Microglobulin