title: Quantitative analysis of epinephrine in human plasma samples using kinetic fluorometric method combined with second-order calibration.
abstract: A kinetic fluorometric method was proposed for the quantitative determination of epinephrine (EP) in human plasma samples with the aid of second-order calibration methods based on alternating trilinear decomposition (ATLD) and alternating fitting residue (AFR) algorithms. It was based on that EP could be gradually converted to a highly fluorescent intermediate product by an oxidation reaction, and further to a non-fluorescent degradation product (o-quinone). These methodologies fully exploit the second-order advantage of the employed three-way kinetic fluorescence data, allowing the concentrations of EP to be quantified even in the presence of uncalibrated interferences. The average recoveries obtained from ATLD and AFR with a factor number of 2 (N = 2) were 100.7 +/- 3.3 and 100.4 +/- 2.2%, respectively. In addition, elliptical joint confidence region (EJCR) tests as well as figures of merit (FOM) were employed to evaluate the accuracy of the two algorithms.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Li
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wu
authors.forename: A-Lin
authors.initials: AL
authors.lastname: Xia
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Zhu
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Nie
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Yu
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D000465:Algorithms; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D004837:Epinephrine / Q000097:blood* / Q000737:chemistry; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D007700:Kinetics; D057230:Limit of Detection; D011809:Quinones / Q000737:chemistry
chemical_list: D011809:Quinones; D004837:Epinephrine