The effect of testosterone replacement therapy on bladder functions and histology in orchiectomized mature male rats.
OBJECTIVE
To investigate the effect of testosterone replacement therapy on bladder functions and smooth muscle/collagen content in orchidectomized orchiectomized mature male rats.


METHODS
The study included 25 mature male Sprague-Dawley rats divided into 3 groups. After bilateral orchiectomy, 8 rats received intramuscular saline injection, as a control group, and 8 rats received intramuscular injection of testosterone undecanoate 100 mg/kg as a treatment group. The sham group had 9 rats. Urodynamic studies were performed in all groups, before and after the study. The rats were killed after 60 days, and cystometric findings and smooth muscle/collagen ratio of the bladders were compared between the groups.


RESULTS
From the beginning to the end of the experiment, mean maximal bladder capacity increased 46.61% +/- 20.82 in the testosterone treatment group, while decreased 38.91% +/- 17.83 in control group, revealing a significant difference (P = .002). Smooth muscle/collagen ratio was significantly higher in the testosterone treatment group (1.53 +/- .34) than in the control group (1.05 +/- .32), (P = .01).


CONCLUSIONS
This study showed that bladder capacity and smooth muscle/collagen content improved with testosterone therapy in orchiectomized rats. Therefore, testosterone replacement therapy in late-onset hypogonadal men with urogenital dysfunction may have a positive role to improve bladder function by increasing bladder smooth muscle.
Tek
Mesut
M
Department of Urology, University of Mersin School of Medicine, Mersin, Turkey.
Balli
Ebru
E
Cimen
Burak
B
Efesoy
Ozan
O
Oğuz
Izzet
I
Cayan
Selahittin
S
D000818:Animals; D020249:Hormone Replacement Therapy; D008297:Male; D009919:Orchiectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology*; D001743:Urinary Bladder / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*
D013739:Testosterone