title: The evolution of two homologues of the core protein VP6 of epizootic haemorrhagic disease virus (EHDV), which correspond to the geographical origin of the virus.
abstract: Epizootic haemorrhagic disease virus is a 10-segmented, double-stranded RNA virus. When these ten segments of dsRNA are run on 1% agarose, eastern (Australia, Japan) and western (North America, Africa, Middle-East) strains of the virus can be separated phenotypically based on the migration of genome segments 7-9. In western strains, segments 7-9 are roughly the same size and co-migrate as a single RNA band. In eastern strains, segment 9 is smaller, so while segments 7 and 8 co-migrate, the segment 9 RNA runs faster than its western homologue. Translation experiments demonstrated that these two segment 9 homologues are both functional and produce proteins (VP6) of different sizes-something that has not been reported in any other orbivirus species to date. Sequence analysis suggests that eastern and western versions of segment 9 (VP6) have likely evolved as a response to adaptive selection in different geographical regions via gene duplication and subsequent mutation. These significant findings are considered unusual given the conserved nature of VP6 and its presumed role as the viral helicase. It is not currently known what the biological relevance of each homologue is to the virus.
authors.affiliation: Vector-Borne Diseases Program, Institute for Animal Health, Ash Road, Pirbright, Surrey, GU24 0NF, UK. santhony@sandiegozoo.org
authors.forename: S J
authors.initials: SJ
authors.lastname: Anthony
authors.forename: K E
authors.initials: KE
authors.lastname: Darpel
authors.forename: S
authors.initials: S
authors.lastname: Maan
authors.forename: G
authors.initials: G
authors.lastname: Sutton
authors.forename: H
authors.initials: H
authors.lastname: Attoui
authors.forename: P P C
authors.initials: PP
authors.lastname: Mertens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D017124:Conserved Sequence; D006224:Cricetinae; D019143:Evolution, Molecular; D016679:Genome, Viral; D018164:Hemorrhagic Disease Virus, Epizootic / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D036022:Capsid Proteins