title: Antifungal mechanism of a novel antifungal protein from pumpkin rinds against various fungal pathogens.
abstract: A novel antifungal protein (Pr-2) was identified from pumpkin rinds using water-soluble extraction, ultrafiltration, cation exchange chromatography, and reverse-phase high-performance liquid chromatography. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry indicated that the protein had a molecular mass of 14865.57 Da. Automated Edman degradation showed that the N-terminal sequence of Pr-2 was QGIGVGDNDGKRGKR-. The Pr-2 protein strongly inhibited in vitro growth of Botrytis cinerea, Colletotrichum coccodes, Fusarium solani, Fusarium oxysporum, and Trichoderma harzianum at 10-20 microM. The results of confocal laser scanning microscopy and SYTOX Green uptake demonstrated that its effective region was the membrane of the fungal cell surface. In addition, this protein was found to be noncytotoxic and heat-stable. Taken together, the results of this study indicate that Pr-2 is a good candidate for use as a natural antifungal agent.
authors.affiliation: Research Center for Proteineous Materials (RCPM), Chosun University, Kwangju, Korea.
authors.forename: Seong-Cheol
authors.initials: SC
authors.lastname: Park
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jong-Kook
authors.initials: JK
authors.lastname: Lee
authors.forename: Indeok
authors.initials: I
authors.lastname: Hwang
authors.forename: Hyeonsook
authors.initials: H
authors.lastname: Cheong
authors.forename: Jae-Woon
authors.initials: JW
authors.lastname: Nah
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D020171:Botrytis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D020231:Colletotrichum / Q000187:drug effects; D028464:Cucurbita / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014242:Trichoderma / Q000187:drug effects
chemical_list: D005659:Fungicides, Industrial; D010940:Plant Proteins