title: Microarray-based identification of gonad transcripts differentially expressed between lines of Pacific oyster selected to be resistant or susceptible to summer mortality.
abstract: Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality.
authors.affiliation: UMR M100 Ifremer- Université de Caen Basse Normandie "Physiologie et Ecophysiologie des Mollusques Marins", Centre de Brest, B.P. 70, 29280 Plouzané/IBFA, IFR ICORE 146, 14032 Caen, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Fleury
authors.forename: Jeanne
authors.initials: J
authors.lastname: Moal
authors.forename: Viviane
authors.initials: V
authors.lastname: Boulo
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Daniel
authors.forename: David
authors.initials: D
authors.lastname: Mazurais
authors.forename: Alain
authors.initials: A
authors.lastname: Hénaut
authors.forename: Charlotte
authors.initials: C
authors.lastname: Corporeau
authors.forename: Pierre
authors.initials: P
authors.lastname: Boudry
authors.forename: Pascal
authors.initials: P
authors.lastname: Favrel
authors.forename: Arnaud
authors.initials: A
authors.lastname: Huvet
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D006066:Gonads / Q000378:metabolism*; D006358:Hot Temperature; D020411:Oligonucleotide Array Sequence Analysis; D010125:Ostreidae / Q000235:genetics* / Q000378:metabolism*; D015203:Reproducibility of Results; D012621:Seasons; D013997:Time Factors