title: Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection.
abstract: A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.
authors.affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Jilin
authors.initials: J
authors.lastname: Cheng
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Tsui-Fen
authors.initials: TF
authors.lastname: Chou
authors.forename: Brahma
authors.initials: B
authors.lastname: Ghosh
authors.forename: Baoling
authors.initials: B
authors.lastname: Liu
authors.forename: Carston R
authors.initials: CR
authors.lastname: Wagner
mesh_terms: D000577:Amides / Q000737:chemistry*; D000596:Amino Acids / Q000378:metabolism; D019380:Anti-HIV Agents / Q000378:metabolism; D017123:Bacteriophage T7 / Q000378:metabolism*; D003561:Cytarabine / Q000494:pharmacology; D016503:Drug Delivery Systems; D015195:Drug Design; D006801:Humans; D019151:Peptide Library; D010756:Phosphoric Acids / Q000737:chemistry*; D014779:Virus Replication; D015215:Zidovudine / Q000378:metabolism*
chemical_list: D000577:Amides; D000596:Amino Acids; D019380:Anti-HIV Agents; D019151:Peptide Library; D010756:Phosphoric Acids; D003561:Cytarabine; D015215:Zidovudine; C011067:phosphoramidic acid