title: In vitro immunomodulatory effects of fractions obtained from aqueous extracts of Larrea divaricata Cav (Jarilla) on mouse peritoneal macrophages.
abstract: OBJECTIVE
  Larrea divaricata Cav. (Zygophyllaceae) is a plant widely used in Argentina.
  
  
  METHODS
  We isolated different fractions of L. divaricata aqueous extract containing minor amounts of NDGA, and we analyzed these fractions on mouse macrophages.
  
  
  RESULTS
  We showed that a fraction without NDGA was capableof activating macrophages, principally through the production of mitochondrial anion superoxide and H(2)O(2). This could be important in the defense of infections. Moreover, this fraction decreased NO level suggesting an anti-inflammatory action.
  
  
  CONCLUSIONS
  These results indicate that NDGA was not the compound responsible for the immunomodulatory action exerted by the aqueous extract from L. divaricata.
authors.affiliation: Microbiology Section, Department of Biochemistry and Biological Science, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, San Luis, Argentina.
authors.forename: Renzo F
authors.initials: RF
authors.lastname: Martino
authors.forename: Roberto C
authors.initials: RC
authors.lastname: Davicino
authors.forename: María A
authors.initials: MA
authors.lastname: Mattar
authors.forename: Yolanda A
authors.initials: YA
authors.lastname: Casali
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Correa
authors.forename: Claudia
authors.initials: C
authors.lastname: Anesini
authors.forename: Blas
authors.initials: B
authors.lastname: Micalizzi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002855:Chromatography, Thin Layer; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology*; D032442:Larrea / Q000737:chemistry; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D007155:Immunologic Factors; D018950:Lipopolysaccharide Receptors; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D009637:Masoprocol