title: Timing of serum soluble HLA-G levels in acute and subacute phases after spontaneous intracerebral hemorrhage.
abstract: Serum levels of sHLA-G (sHLA-G1/HLA-G5) antigens and their soluble isoforms, sHLA-G1 and HLA-G5, were measured by ELISA in 22 patients with spontaneous intracerebral hemorrhage (SICH) at 24 h, 48 h and 7 days after bleeding. The perihematomal edema volume was calculated on non-enhanced computed tomography scans using the formula AxBxC/2 at the same time points. The mean serum concentrations of sHLA-G1/HLA-G5 and sHLA-G1 as well as the perihematomal edema volume changed significantly over time (p < 0.0001, p < 0.001 and p < 0.0001, respectively), whereas no statistical differences were found in serum HLA-G5 concentrations over the course of the experiment. In comparison to the values found at 24 h, sHLA-G1/HLA-G5 and sHLA-G1 increased at 48 h and then decreased at 7 days, whereas the perihematomal edema volume was more elevated at 48 h and, to a lesser extent, at 7 days. A positive correlation was detected between mean serum sHLA-G1/HLA-G5 and sHLA-G1 levels and perihematomal edema volume at 24 h (p < 0.02) and at 48 h (p < 0.01). Our results may indicate a role for sHLA-G in inflammatory mechanisms related to SICH, where these proteins probably act as anti-inflammatory molecules and are predominantly produced as the sHLA-G1 isoform.
authors.affiliation: Neuroradiology Unit, Department of Neurosciences and Rehabilitation, Azienda Ospedaliera-Universitaria, Ferrara, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Fainardi
authors.forename: Roberta
authors.initials: R
authors.lastname: Rizzo
authors.forename: Angelica
authors.initials: A
authors.lastname: Lupato
authors.forename: Vania
authors.initials: V
authors.lastname: Ramponi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: De Santis
authors.forename: Francesco
authors.initials: F
authors.lastname: Garofano
authors.forename: Marina
authors.initials: M
authors.lastname: Stignani
authors.forename: Carmine
authors.initials: C
authors.lastname: Tamborino
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Castellazzi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Casetta
authors.forename: Olavio Roberto
authors.initials: OR
authors.lastname: Baricordi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001929:Brain Edema / Q000209:etiology; D002543:Cerebral Hemorrhage / Q000097:blood* / Q000150:complications / Q000000981:diagnostic imaging; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006680:HLA Antigens / Q000097:blood*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I