[Preparation and identification of polyclonal antibody against protein H1b: the variant of major subunit of human ASGPR].
OBJECTIVE
To prepare and identify mouse polyclonal antibody against protein H1b, which is the variant of major subunit of human ASGPR.


METHODS
H1b specific peptide was synthesized and coupled with keyhole limpet hemocyanin (KLH) for immunization. Then H1b-KLH conjugation was injected into mouse subcutaneously to produce polyclonal antibody. ELISA assay was used to detect the titer of the antibody. Antibody was also identified by Western blot and immunohistochemistry assays.


RESULTS
Mouse antibody against H1b was prepared after injection of H1b-KLH conjugation. The titer of H1b antibody was about 1:10(5). Western blot confirmed its high specificity. This antibody could also be used for immunohistochemistry analysis.


CONCLUSIONS
The successful preparation of the polyclonal antibody against protein H1b, which can discriminate the two variants of the major subunit of ASGPR with high specificity, will provide an efficient reagent for further study of the physiologic functions of H1b and its role in the pathogenesis of human disease.
Liu
Jia
J
Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. jialiu77@hotmail.com
Ding
Hong-Hui
HH
Yang
Yan
Y
Hu
Bin
B
Yu
Yuan
Y
Huang
Hong-Ping
HP
Lu
Meng-Ji
MJ
Yang
Dong-Liang
DL
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D037263:Asialoglycoprotein Receptor / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C
C546927:ASGR1 protein, human; D000906:Antibodies; D037263:Asialoglycoprotein Receptor