title: Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats.
abstract: Growth patterns of Cryptococcus neoformans submerged culture in different culture volumes, intensity of agitation and types of sealing were evaluated to better understand the physiological role of hypoxia response in this yeast. When low intensity agitation was set at high culture volumes and air exchange between the cultivation vessel and external environment was not abolished completely, the cells proliferated slowly but steadily. On the other hand, when the intensity of agitation was high but the vessel was withheld from fresh air supply, the cells first proliferated rapidly, then arrested completely and finally died. Therefore, the central strategy of C. neoformans here seems to lie in its proliferation-rate adjustment to the available oxygen levels and not in its capacity to survive under anoxia. The data support the opinion that the cultures grown under limited aeration (even though not completely withheld from fresh air supply) are much closer to the real cryptococcal life in human tissues than conventional well-aerated exponential cultures.
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, 775 15 Olomouc, Czech Republic. rac@tunw.upol.cz
authors.forename: V
authors.initials: V
authors.lastname: Raclavský
authors.forename: V
authors.initials: V
authors.lastname: Husicková
authors.forename: Z
authors.initials: Z
authors.lastname: Moránová
authors.forename: M
authors.initials: M
authors.lastname: Ohkusu
authors.forename: O
authors.initials: O
authors.lastname: Fischer
authors.forename: J
authors.initials: J
authors.lastname: Precek
authors.forename: J
authors.initials: J
authors.lastname: Trtková
authors.forename: K
authors.initials: K
authors.lastname: Takeo
authors.forename: S
authors.initials: S
authors.lastname: Kawamoto
mesh_terms: D003453:Cryptococcosis / Q000382:microbiology*; D003455:Cryptococcus neoformans / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D050296:Microbial Viability; D010100:Oxygen / Q000378:metabolism
chemical_list: D003470:Culture Media; D010100:Oxygen