Pyrene-labeled deoxyguanosine as a fluorescence sensor to discriminate single and double stranded DNA structures: design of ends free molecular beacons.
A novel fluorescent DNA probe containing pyrene-labeled C8 alkylamino-substituted 2'-deoxyguanosine was designed in order to discriminate single stranded and double stranded regions in DNA. This fluorescent sensor was used for the design of practically useful 3'- and 5'-ends free self-quenched molecular beacon (MB). Unique MB detectable by pyrene excimer fluorescence was also demonstrated.
Matsumoto
Katsuhiko
K
Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
Shinohara
Yuta
Y
Bag
Subhendu S
SS
Takeuchi
Yoshiki
Y
Morii
Takashi
T
Saito
Yoshio
Y
Saito
Isao
I
D002273:Carcinogens / Q000633:toxicity; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D005453:Fluorescence; D011721:Pyrenes / Q000737:chemistry*
D002273:Carcinogens; D015342:DNA Probes; D011721:Pyrenes; D004247:DNA