Hyperoxia exposure alters hepatic eicosanoid metabolism in newborn mice.
Prematurely born infants are often treated with supraphysiologic amounts of oxygen, which is associated with lung injury and the development of diseases such as bronchopulmonary dysplasia. Complimentary responses between the lung and liver during the course of hyperoxic lung injury have been studied in adult animals, but little is known about this relationship in neonates. These studies tested the hypothesis that oxidant stress occurs in the livers of newborn mice in response to continuous hyperoxia exposure. Greater levels of glutathione disulfide and nitrotyrosine were detected in lung tissues but not liver tissues from newborn mice exposed to hyperoxia than in room air-exposed controls. However, early increases in 5-lipoxygenase and cyclooxygenases-2 protein levels and increases in total hydroxyeicosatetraenoic acid and prostaglandin levels were observed in the liver tissues of hyperoxia-exposed pups. These studies indicate that free radical oxidation occurs in the lungs of newborn pups exposed to hyperoxia, and alterations in lipid metabolism could be a primary response in the liver tissues. The findings of this study identify possible new mechanisms associated with hyperoxic lung injury in a newborn model of bronchopulmonary dysplasia and thus open opportunities for research.
Rogers
Lynette K
LK
Center for Perinatal Research, Department of Pediatrics, The Research Institute at Nationwide Children's Hospital, Ohio State University, 700 Children's Dr., Columbus, Ohio 43205, USA. Lynette.Rogers@NationwideChildrens.org
Tipple
Trent E
TE
Britt
Rodney D
RD
Welty
Stephen E
SE
D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D019803:Glutathione Disulfide / Q000378:metabolism; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D018496:Hyperoxia / Q000150:complications / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D018384:Oxidative Stress; D011453:Prostaglandins / Q000378:metabolism*; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; C002744:3-nitrotyrosine; D014443:Tyrosine; C019305:coenzyme A-glutathione mixed disulfide; D001094:Arachidonate 5-Lipoxygenase; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D003065:Coenzyme A; D019803:Glutathione Disulfide