[Identification of genes over-expressed in myxoid/round cell liposarcoma. DNA microarray analysis and immunohistochemical correlation].
Myxoid/round cell liposarcoma are characterized by typical chromosomal translocations. This genetic alteration might result in specific gene-expression profiles in this tumor entity. To identify over-expressed genes in myxoid/round cell liposarcoma DNA microarray analysis was performed on four tumors and four samples of adult fat tissue. Genes ret, cdk4, cyclin D2 and c-myc showed over-expression by means of microarray analysis and Northern blotting. Immunohistochemistry demonstrated cytoplasmic localization of associated proteins in 36 different tumors. The localization of ret was seen in endothelial cells of plexiform vasculature in addition to its accumulation in tumor cells (25% of cases). The results show an over-expression of cdk4, cyclin D2, c-myc and ret on both the transcriptional and protein level in myxoid/round cell liposarcoma. For cyclin D2 and ret this finding has not been reported in this tumor type. The increase of ret on transcriptional level might be explained by its expression in endothelium in intratumoral plexiform blood vessels. For the molecular pathogenesis of myxoid/round cell liposarcoma our findings imply the involvement of these four genes in the deregulation of the cell cycle, especially as cdk4 and cyclin D2 are target genes of c-myc.
Lanckohr
C
C
Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.
Kasprzynski
A
A
Klein-Hitpass
L
L
Homann
H-H
HH
Lehnhardt
M
M
Müller
O
O
Kuhnen
C
C
Herter
P
P
D014408:Biomarkers, Tumor / Q000235:genetics*; D056742:Cyclin D2 / Q000235:genetics; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008080:Liposarcoma / Q000145:classification / Q000235:genetics* / Q000473:pathology*; D018208:Liposarcoma, Myxoid / Q000145:classification / Q000235:genetics* / Q000473:pathology*; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics; D012983:Soft Tissue Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology*; D014178:Translocation, Genetic / Q000235:genetics
D014408:Biomarkers, Tumor; C080336:CCND2 protein, human; D056742:Cyclin D2; D005819:Genetic Markers; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D051096:Proto-Oncogene Proteins c-ret; C099282:RET protein, human; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4