title: PP13 mRNA expression in the cellular component of maternal blood as a marker for preeclampsia.
abstract: OBJECTIVE
  To assess the PP13 expression in the cellular component of blood in both preeclamptic patients and asymptomatic pregnant women during the early second trimester.
  
  
  METHODS
  In the case-control study, peripheral blood samples were obtained from pregnant women with preeclampsia (n = 24) and controls (n = 22). PP13 RNA expression was quantified in the cellular component of the blood by reverse transcription PCR assay. Next, as a cohort study of asymptomatic pregnant women at early gestation, cellular RNA from 41 cases who developed preeclampsia at later gestation and 123 cases of control were analysed, and the possibility of prediction of preeclampsia was assessed.
  
  
  RESULTS
  In symptomatic patients, PP13 level in preeclampsia was significantly lower than that in controls (p < 0.001). In the asymptomatic pregnant women, the PP13 level in preeclampsia was significantly lower than that in the controls (p = 0.008). A receiver-operating characteristics (ROC) curve yielded a detection rate of 24 and 31% at a false positive rate of 5 and 10% respectively.
  
  
  CONCLUSIONS
  An alteration in the cellular PP13 RNA expression would affect the placental pathophysiological change. The cellular PP13 expression level could therefore potentially be one of the key markers to predict the clinical onset of preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Hanako
authors.initials: H
authors.lastname: Shimizu
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
authors.forename: Yuditiya
authors.initials: Y
authors.lastname: Purwosunu
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Antonio
authors.initials: A
authors.lastname: Farina
authors.forename: Nicola
authors.initials: N
authors.lastname: Rizzo
authors.forename: Takashi
authors.initials: T
authors.lastname: Okai
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001773:Blood Cells / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D037161:Galectins / Q000097:blood*; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D037161:Galectins; C038195:LGALS13 protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger