Bone morphogenetic protein-6-loaded chitosan scaffolds enhance the osteoblastic characteristics of MC3T3-E1 cells.
The purpose of this study is to investigate the convenience of bone morphogenetic protein-6 (BMP-6)-loaded chitosan scaffolds with preosteoblastic cells for bone tissue engineering. MC3T3-E1 cells were seeded into three different groups: chitosan scaffolds, BMP-6-loaded chitosan scaffolds, and chitosan scaffolds with free BMP-6 in culture medium. Tissue-engineered constructs were characterized by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay, scanning electron microscopy (SEM), mineralization assay (von Kossa), alkaline phosphatase (ALP) activity, and osteocalcin (OCN) assays. BMP-6-loaded chitosan scaffolds supported proliferation of the MC3T3-E1 mouse osteogenic cells in a similar pattern as the unloaded chitosan scaffolds group and as the chitosan scaffolds with free BMP-6 group. SEM images of the cell-seeded scaffolds revealed significant acceleration of extracellular matrix synthesis in BMP-6-loaded chitosan scaffolds. Both levels of ALP and OCN were higher in BMP-6-loaded chitosan scaffold group compared with the other two groups. In addition, BMP-6-loaded scaffolds showed strong staining in mineralization assays. These findings suggest that BMP-6-loaded chitosan scaffold supports cellular functions of the osteoblastic cells; therefore, this scaffold is considered as a new promising vehicle for bone tissue engineering applications.
Akman
Abdullah C
AC
Department of Periodontology, Faculty of Dentistry, Hacettepe University, Beytepe, Ankara, Turkey.
Seda Tiğli
R
R
Gümüşderelioğlu
Menemşe
M
Nohutcu
Rahime M
RM
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055418:Bone Morphogenetic Protein 6 / Q000502:physiology*; D001842:Bone and Bones / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds
C525816:BMP6 protein, human; D055418:Bone Morphogenetic Protein 6; D015675:Osteocalcin; D048271:Chitosan; D000469:Alkaline Phosphatase