Can ultrasonic energy efficiently speed (18)O-labeling of proteins?
We report in this work on the robustness of ultrasonic energy as a tool to speed the isotopic labeling of proteins using the (18)O-decoupling procedure. The first part of the decoupling procedure, comprising protein denaturation, reduction, alkylation and digestion, is done in 8 min under the effects of an ultrasonic field whilst the second part, the isotopic labeling, was assayed with and without the use of ultrasonic energy. Our results clearly demonstrate that the (18)O-isotopic labeling in a decoupling procedure cannot be accelerated using an ultrasonic field.
Carreira
Ricardo J
RJ
REQUIMTE, Departamento de Química, Centro de Química Fina e Biotecnologia, Faculdade de Ciênciase Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, Monte de Caparica, Portugal.
Lodeiro
Carlos
C
Diniz
Mario S
MS
Moura
Isabel
I
Capelo
José L
JL
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D007553:Isotope Labeling / Q000379:methods*; D007768:Lactalbumin / Q000032:analysis* / Q000737:chemistry; D008969:Molecular Sequence Data; D010103:Oxygen Isotopes / Q000032:analysis; D040901:Proteomics; D012710:Serum Albumin, Bovine / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014465:Ultrasonics
D010103:Oxygen Isotopes; D012710:Serum Albumin, Bovine; D007768:Lactalbumin