title: Investigation of Klebsiella pneumoniae isolates producing SHV-12 and SHV-11 beta-lactamases in Korean hospitals.
abstract: Of 143 clinical isolates of Klebsiella pneumoniae collected from Korean non-tertiary hospitals, 24 (16.8%) showed an extended-spectrum beta-lactamase-positive phenotype. PCR and sequence analysis revealed the presence of TEM-116 (n=13), CTX-M-3 (n=5), CTX-M-14 (n=2), CTX-M-15 (n=3), and SHV-12 (n=16). Each of the 24 isolates encoded more than one beta-lactamase, and seven isolates (29%) harbored two different SHV-type beta-lactamase genes (blaSHV-11 and blaSHV-12) bounded by insertion sequence IS26 in a single transferable plasmid.
authors.affiliation: Division of Antimicrobial Resistance, National Institute of Health, 194 Tongil-Lo, Eunpyung-Gu, Seoul 122-701, Korea.
authors.forename: Kyeong Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Yoo
authors.forename: Yong Sun
authors.initials: YS
authors.lastname: Yoo
authors.forename: Jung Sik
authors.initials: JS
authors.lastname: Yoo
authors.forename: Gyung Tae
authors.initials: GT
authors.lastname: Chung
authors.forename: Tae In
authors.initials: TI
authors.lastname: Ahn
authors.forename: Yeong Seon
authors.initials: YS
authors.lastname: Lee
mesh_terms: D015139:Blotting, Southern / Q000379:methods; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006761:Hospitals / Q000592:standards; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000453:epidemiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D007723:Korea; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D013183:Sputum / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; C107197:beta-lactamase SHV-11; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases