title: Enhancement of immune responses to Newcastle disease vaccine by a supplement of extract of Momordica cochinchinensis (Lour.) Spreng. seeds.
abstract: The study evaluated the immunological effect of extract of Momordica cochinchinensis (Lour.) Spreng. seeds (ECMS) on the immune response against Newcastle disease (ND) in chickens. Forty-eight chickens were divided into 4 groups (n = 12). Each chicken was immunized with ND vaccine mixed with 0, 20, 40, or 80 microg of ECMS on d 35. Blood samples were collected on d 0, 7, 14, 21, 28, and 35 postimmunization. Humoral and cellular immune responses were evaluated by indirect ELISA assay and the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Results indicated that humoral immune response was enhanced by ECMS 14 d postimmunization. Eighty micrograms of ECMS was the best dose with the ND vaccine and was significantly different from the other groups 21 d after immunization. No significant differences were found in the cellular immune response, whereas the 80 microg of ECMS group had higher values than the other groups 35 d after immunization. No side effect was found on the growth performance during the experiment.
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, P. R. China.
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: G
authors.initials: G
authors.lastname: Bao
authors.forename: S
authors.initials: S
authors.lastname: Hu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D007074:Immunoglobulin G / Q000097:blood; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D008934:Mitogens; D029775:Momordica / Q000737:chemistry*; D009521:Newcastle Disease / Q000517:prevention & control*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014765:Viral Vaccines / Q000276:immunology*; D015430:Weight Gain
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D008934:Mitogens; D010936:Plant Extracts; D014765:Viral Vaccines