Identification of potential DREB2C targets in Arabidopsis thaliana plants overexpressing DREB2C using proteomic analysis.
The dehydration responsive element binding protein 2C (DREB2C) is a dehydration responsive element/C-repeat (DRE/CRT)-motif binding transcription factor that induced by mild heat stress. Previous experiments established that overexpression of DREB2C cDNA driven by the cauliflower mosaic virus 35S promoter (35S:DREB2C) resulted in increased heat tolerance in Arabidopsis. We first analyzed the proteomic profiles in wild-type and 35S:DREB2C plants at a normal temperature (22 degrees C), but could not detect any differences between the proteomes of wild-type and 35S:DREB2C plants. The transcript level of DREB2C in 35S:DREB2C plants after treatment with mild heat stress was increased more than two times compared with expression in 35S:DREB2C plants under unstressed condition. A proteomic approach was used to decipher the molecular mechanisms underlying thermotolerance in 35S:DREB2C Arabidopsis plants. Eleven protein spots were identified as being differentially regulated in 35S:DREB2C plants. Moreover, in silico motif analysis showed that peptidyl-prolyl isomerase ROC4, glutathione transferase 8, pyridoxal biosynthesis protein PDX1, and elongation factor Tu contained one or more DRE/CRT motifs. To our knowledge, this study is the first to identify possible targets of DREB2C transcription factors at the protein level. The proteomic results were in agreement with transcriptional data.
Lee
Kyunghee
K
The Aging-associated Vascular Disease Research Center and Department of Microbiology, Yeungnam University College of Medicine, Daegu 705-717, Korea.
Han
Ki Soo
KS
Kwon
Young Sang
YS
Lee
Jung Han
JH
Kim
Sun Ho
SH
Chung
Woo Sik
WS
Kim
Yujung
Y
Chun
Sung-Sik
SS
Kim
Hee Kyu
HK
Bae
Dong-Won
DW
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000235:genetics; D003681:Dehydration / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics
D029681:Arabidopsis Proteins; C114579:DREB2A protein, Arabidopsis; D012333:RNA, Messenger; D014157:Transcription Factors