7-Amino actinomycin D bound to single-stranded hairpin DNA enhanced by loop sequence-dependent luminescent Eu(3+) and Tb(3+) binding.
Lanthanide Eu(3+) and Tb(3+) ions have been widely used in luminescent resonance energy transfer (LRET) for bioassays to study metal binding microenvironments. We report here that Eu(3+) or Tb(3+) can increase the binding affinity of antitumor antibiotic drug agent, 7-amino actinomycin D (7AACTD), binding to 5'-GT/TG-5' or 5'-GA/AG-5' mismatched stem region of the single-stranded hairpin DNA. Further studies indicate that the effect of Eu(3+) or Tb(3+) on 7AACTD binding is related to DNA loop sequence. Our results will provide new insights into how metal ions can enhance antitumor agents binding to their targets.
Peng
Yinghua
Y
Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Graduate School of the Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Song
Yujun
Y
Feng
Lingyan
L
Ren
Jinsong
J
Qu
Xiaogang
X
D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D002942:Circular Dichroism; D004277:DNA, Single-Stranded / Q000737:chemistry*; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000737:chemistry; D005063:Europium / Q000737:chemistry*; D055029:Inverted Repeat Sequences; D008163:Luminescent Measurements; D009690:Nucleic Acid Conformation; D013725:Terbium / Q000737:chemistry*
D000903:Antibiotics, Antineoplastic; D004277:DNA, Single-Stranded; D013725:Terbium; D003609:Dactinomycin; D005063:Europium; C025942:7-aminoactinomycin D