Molecular proteomics imaging of tumor interfaces by mass spectrometry.
The specific molecular profiles of ovarian cancer interface zones (IZ), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Two-dimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interface-specific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDI-IMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors.
Kang
Suki
S
Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
Shim
Hyo Sup
HS
Lee
Jong Sik
JS
Kim
Dong Su
DS
Kim
Hak Yong
HY
Hong
Seong Hyun
SH
Kim
Pan Soo
PS
Yoon
Joo Heon
JH
Cho
Nam Hoon
NH
D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D010051:Ovarian Neoplasms / Q000737:chemistry*; D025341:Principal Component Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*