MicroRNA 125a and its regulation of the p53 tumor suppressor gene.
MicroRNA (miRNA) are a class of non-coding RNA that suppress gene expression by degradation or translational inhibition of target RNA. Several miRNA have been shown to target oncogenes and recently miRNA-125b was shown to translationally and transcriptionally inhibit the p53 gene. Here, we show that an additional isomer of miRNA-125 (miRNA-125a) translationally arrests mRNA of the p53 tumor suppressor gene. The basis of this activity is the high degree of sequence homology between the seed sequence of miR-125a and the 3'-UTR of p53. Our findings add miRNA-125a to the growing list of miRNA with oncogenic targets.
Zhang
Yingjie
Y
Division of Infectious Diseases, Department of Medicine, Laboratory of Retrovirology, Warren Alpert Medical School of Brown University, Providence, RI 02903, USA.
Gao
Jin-Song
JS
Tang
Xiaoli
X
Tucker
Lynne D
LD
Quesenberry
Peter
P
Rigoutsos
Isidore
I
Ramratnam
Bharat
B
D020413:3' Untranslated Regions / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D014176:Protein Biosynthesis; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
D020413:3' Untranslated Regions; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53