title: Ameyamaea chiangmaiensis gen. nov., sp. nov., an acetic acid bacterium in the alpha-Proteobacteria.
abstract: Two isolates, AC04(T) and AC05, were isolated from the flowers of red ginger collected in Chiang Mai, Thailand. In phylogenetic trees based on 16S rRNA gene sequences, the two isolates were included within a lineage comprised of the genera Acidomonas, Gluconacetobacter, Asaia, Kozakia, Swaminathania, Neoasaia, Granulibacter, and Tanticharoenia, and they formed an independent cluster along with the type strain of Tanticharoenia sakaeratensis. The calculated pair-wise sequence similarities of isolate AC04(T) were 97.8-92.5% to the type strains of the type species of the 11 genera of acetic acid bacteria. The DNA base composition was 66.0-66.1 mol % G+C with a range of 0.1 mol %. A single-stranded, labeled DNA from isolate AC04(T) presented levels of DNA-DNA hybridization of 100, 85, 4, and 3% respectively to DNAs from isolates AC04(T) and AC05 and the type strains of Tanticharoenia sakaeratensis and Gluconacetobacter liquefaciens. The two isolates were unique morphologically in polar flagellation and physiologically in intense acetate oxidation to carbon dioxide and water and weak lactate oxidation. The intensity in acetate oxidation almost equaled that of the type strain of Acetobacter aceti. The two isolates had Q-10. Isolate AC04(T) was discriminated from the type strains of the type species of the 11 genera by 16S rRNA gene restriction analysis using restriction endonucleases TaqI and Hin6I. The unique phylogenetic, genetic, morphological, physiological, and biochemical characteristics obtained indicate that the two isolates can be classified into a separate genus, and Ameyamaea chiangmaiensis gen. nov., sp. nov. is proposed. The type strain is isolate AC04(T) (=BCC 15744(T), =NBRC 103196(T)), which has a DNA G+C content of 66.0 mol %.
authors.affiliation: BIOTEC Culture Collection, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand.
authors.forename: Pattaraporn
authors.initials: P
authors.lastname: Yukphan
authors.forename: Taweesak
authors.initials: T
authors.lastname: Malimas
authors.forename: Yuki
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Mai
authors.initials: M
authors.lastname: Takahashi
authors.forename: Mika
authors.initials: M
authors.lastname: Kaneyasu
authors.forename: Wanchern
authors.initials: W
authors.lastname: Potacharoen
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Koei
authors.initials: K
authors.lastname: Hamana
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Tahara
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Morakot
authors.initials: M
authors.lastname: Tanticharoen
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D016947:Acetobacteraceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019342:Acetic Acid