A new paraneoplastic encephalomyelitis autoantibody reactive with the axon initial segment.
Serum from a patient with paraneoplastic encephalomyelitis (PEM) and small cell lung cancer (SCLC) showed high titer immunohistochemical staining of the axon initial segment (AIS) on rat and human brain sections. EM studies showed that the antigen was localized in close proximity of the microtubules in the AIS. Double labeling experiments and absence of staining at the nodes of Ranvier excluded the previously identified betaIV spectrin as autoantigen. Screening a rat hippocampal cDNA library resulted in the isolation of ubiquitin-conjugating enzyme E2E1 (UBE2E1). However, blocking and elution experiments excluded UBE2E1 as the AIS autoantigen.
Shams'ili
Setareh
S
Department of Neurology, Erasmus University Medical Center, The Netherlands.
de Leeuw
Bertie
B
Hulsenboom
Esther
E
Jaarsma
Dick
D
Smitt
Peter Sillevis
PS
D000368:Aged; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001369:Axons / Q000276:immunology*; D001921:Brain / Q000276:immunology; D006801:Humans; D008175:Lung Neoplasms / Q000150:complications / Q000276:immunology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000276:immunology; D020361:Paraneoplastic Syndromes, Nervous System / Q000150:complications / Q000276:immunology*; D051381:Rats; D055752:Small Cell Lung Carcinoma / Q000150:complications / Q000276:immunology*; D013049:Spectrin / Q000276:immunology; D044763:Ubiquitin-Conjugating Enzymes / Q000276:immunology
D001323:Autoantibodies; D009419:Nerve Tissue Proteins; C417303:betaIV spectrin; D013049:Spectrin; C489963:UBE2E1 protein, human; D044763:Ubiquitin-Conjugating Enzymes