Complement receptor 3, not Dectin-1, is the major receptor on human neutrophils for beta-glucan-bearing particles.
We investigated the role of the beta-glucan receptor, Dectin-1, in the response of human neutrophils to unopsonized Saccharomyces cerevisiae and its major beta-glucan-containing capsular constituent, zymosan. Although reported to be indispensable for yeast phagocytosis in murine phagocytes, human Dectin-1 was not involved in the phagocytosis of S. cerevisiae or zymosan by human neutrophils. Phagocytosis of yeast particles proved to be completely dependent on CD11b/CD18, also known as complement receptor 3 (CR3). The findings were supported by data with neutrophils from a patient suffering from Leukocyte-Adhesion Deficiency type-1 (LAD-1) syndrome lacking CD11b/CD18. In addition, neither the priming by zymosan of the fMLP-induced NADPH-oxidase activity in human neutrophils nor the secretion of IL-8 by human neutrophils in response to zymosan preparations was affected by blocking anti-Dectin-1 antibodies or laminarin as a monovalent inhibitor. As shown by neutrophils from an IRAK-4-deficient patient, the zymosan-induced IL-8 release was also independent of TLR2. In summary, our data show that Dectin-1, although indispensable for recognition of beta-glucan-bearing particles in mice, is not the major receptor for yeast particles in human neutrophils.
van Bruggen
Robin
R
Sanquin Research, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands. r.vanbruggen@sanquin.nl
Drewniak
Agata
A
Jansen
Machiel
M
van Houdt
Michel
M
Roos
Dirk
D
Chapel
Helen
H
Verhoeven
Arthur J
AJ
Kuijpers
Taco W
TW
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D037181:Lectins, C-Type; D016177:Macrophage-1 Antigen / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis / Q000276:immunology; D016897:Respiratory Burst / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000276:immunology; D015054:Zymosan / Q000276:immunology*; D000099181:Dectin-1
D016207:Cytokines; D006861:Hydrogen Peroxide; D016209:Interleukin-8; D037181:Lectins, C-Type; D016177:Macrophage-1 Antigen; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D015054:Zymosan; D000099181:Dectin-1