title: Preliminary evidence for a matrix metalloproteinase-2 (MMP-2)-dependent shedding of soluble CD40 ligand (sCD40L) from activated platelets.
abstract: Platelets are the major source of soluble CD40 ligand (sCD40L) in the blood. It has been demonstrated that CD40L is cleaved from the surface of activated platelets to release sCD40L. However, the enzyme involved in sCD40L shedding has not been identified yet. Using a panel of pharmacological inhibitors of serine, cysteine, aspartate, or metalloproteinases, preliminary evidence is presented for the hypothesis that matrix metalloproteinase-2 (MMP-2) might be the protease, primarily responsible for CD40L cleavage from platelet surface.
authors.affiliation: Institut für Pharmakologie, Universitätsklinikum Essen, Universität Duisburg-Essen, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Reinboldt
authors.forename: Folker
authors.initials: F
authors.lastname: Wenzel
authors.forename: Bernhard H
authors.initials: BH
authors.lastname: Rauch
authors.forename: Thomas
authors.initials: T
authors.lastname: Hohlfeld
authors.forename: Maria
authors.initials: M
authors.lastname: Grandoch
authors.forename: Jens W
authors.initials: JW
authors.lastname: Fischer
authors.forename: Artur-Aron
authors.initials: AA
authors.lastname: Weber
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D023201:CD40 Ligand / Q000097:blood* / Q000276:immunology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000097:blood* / Q000276:immunology; D061965:Matrix Metalloproteinase Inhibitors; D010446:Peptide Fragments / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology*; D010976:Platelet Count
chemical_list: D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D010446:Peptide Fragments; C082835:thrombin receptor peptide (42-47); D023201:CD40 Ligand; D020778:Matrix Metalloproteinase 2