title: Infection kinetics of human adenovirus serotype 41 in HEK 293 cells.
abstract: The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.
authors.affiliation: Laboratório de Biologia Molecular de Adenovírus, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Joselma
authors.initials: J
authors.lastname: Siqueira-Silva
authors.forename: Fernanda Perez
authors.initials: FP
authors.lastname: Yeda
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Favier
authors.forename: Paulette
authors.initials: P
authors.lastname: Mezin
authors.forename: Misael Leonardo
authors.initials: ML
authors.lastname: Silva
authors.forename: Karina Médici
authors.initials: KM
authors.lastname: Barrella
authors.forename: Dolores Ursula
authors.initials: DU
authors.lastname: Mehnert
authors.forename: Pascal
authors.initials: P
authors.lastname: Fender
authors.forename: Charlotte Marianna
authors.initials: CM
authors.lastname: Hársi
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line / Q000821:virology; D002999:Clone Cells; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D011817:Rabbits; D013997:Time Factors