Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi.
The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.
Imwong
Mallika
M
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Tanomsing
Naowarat
N
Pukrittayakamee
Sasithon
S
Day
Nicholas P J
NP
White
Nicholas J
NJ
Snounou
Georges
G
D000818:Animals; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics; D006801:Humans; D008288:Malaria / Q000175:diagnosis / Q000469:parasitology; D016780:Malaria, Vivax / Q000175:diagnosis / Q000469:parasitology; D016790:Plasmodium knowlesi / Q000235:genetics* / Q000302:isolation & purification; D010966:Plasmodium vivax / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity
D017931:DNA Primers; D016054:DNA, Protozoan; D012335:RNA, Ribosomal