Droplet-based compartmentalization of chemically separated components in two-dimensional separations.
We demonstrate that nanolitre-sized droplets are an effective tool in coupling two-dimensional separations in both time and space. Using a microfluidic droplet connector, chemically separated components can be segmented into nanolitre droplets. After oil filtering and droplet merging, these droplets are loaded into a second dimension for comprehensive separations.
Niu
X Z
XZ
Department of Chemistry, Imperial College London, South Kensington, London, UK SW7 2AZ.
Zhang
B
B
Marszalek
R T
RT
Ces
O
O
Edel
J B
JB
Klug
D R
DR
deMello
A J
AJ
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010455:Peptides / Q000302:isolation & purification*
D010455:Peptides