Sister chromatid exchanges and chromosomal aberrations in Chinese hamster ovary (CHO-K1) cells treated with the insecticide pirimicarb.
Pirimicarb and its formulation Aficida (50% pirimicarb) effects were studied on CHO-K1 cells employing sister chromatid exchange (SCE), chromosomal aberrations (CA), cell-cycle progression and mitotic index analyses. Continuous treatments were performed within 10-300 microg/ml concentration-range. Pirimicarb, but not Aficida, induced a concentration-dependent increase of abnormal cells. Pirimicarb induced a greater frequency of chromatid/isochromatid breaks than Aficida did. Regression analyses showed a concentration-dependent increase in the frequency of chromatid-type breaks for both compounds whereas only the frequency of isochromatid-type breaks did in those pirimicarb-treated cultures. SCEs in pirimicarb- or Aficida-treated cultures were significantly higher than control values with concentrations of 100-200 microg/ml. Both test compounds induced equivalent frequency of SCEs. A delay in cell-cycle kinetics was observed for pirimicarb and Aficida within 100-300 and 200-300 microg/ml concentration-range, respectively. An inhibition of MI was observed for both chemicals regardless of tested concentrations. Finally, the CAs appears to be a higher sensitive bioassay to detect DNA damage at lower concentrations of pirimicarb than SCEs does. The results demonstrated that pirimicarb and Aficida exert geno-cytotoxicity, at least in CHO-K1 cells.
Soloneski
Sonia
S
Laboratorio de Citogenética, Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, 1900 La Plata, Argentina. ssoloneski@yahoo.com.ar
Larramendy
Marcelo L
ML
D000818:Animals; D016466:CHO Cells; D002219:Carbamates / Q000633:toxicity*; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000633:toxicity*; D011743:Pyrimidines / Q000633:toxicity*; D012854:Sister Chromatid Exchange
D002219:Carbamates; D007306:Insecticides; D011743:Pyrimidines; C011994:pirimicarb