title: PDF-modulated visual inputs and cryptochrome define diurnal behavior in Drosophila.
abstract: Morning and evening circadian oscillators control the bimodal activity of Drosophila in light-dark cycles. The lateral neurons evening oscillator (LN-EO) is important for promoting diurnal activity at dusk. We found that the LN-EO autonomously synchronized to light-dark cycles through either the cryptochrome (CRY) that it expressed or the visual system. In conditions in which CRY was not activated, flies depleted for pigment-dispersing factor (PDF) or its receptor lost the evening activity and displayed reversed PER oscillations in the LN-EO. Rescue experiments indicated that normal PER cycling and the presence of evening activity relied on PDF secretion from the large ventral lateral neurons and PDF receptor function in the LN-EO. The LN-EO thus integrates light inputs and PDF signaling to control Drosophila diurnal behavior, revealing a new clock-independent function for PDF.
authors.affiliation: Institut de Neurobiologie Alfred Fessard, Centre National de la Recherche Scientifique (UPR 2216), Gif-sur-Yvette, France.
authors.forename: Paola
authors.initials: P
authors.lastname: Cusumano
authors.forename: André
authors.initials: A
authors.lastname: Klarsfeld
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Chélot
authors.forename: Marie
authors.initials: M
authors.lastname: Picot
authors.forename: Benjamin
authors.initials: B
authors.lastname: Richier
authors.forename: François
authors.initials: F
authors.lastname: Rouyer
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal; D001683:Biological Clocks / Q000235:genetics / Q000502:physiology*; D056931:Cryptochromes / Q000378:metabolism*; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D009043:Motor Activity / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D014795:Visual Pathways / Q000502:physiology*
chemical_list: D056931:Cryptochromes; D029721:Drosophila Proteins; D009479:Neuropeptides; D018013:Receptors, Neuropeptide; C113883:pdf protein, Drosophila; C400531:pigment epithelium-derived factor receptor