title: Expression and localization of acrosin inhibitor in boar reproductive tract.
abstract: Proteinases and proteinase inhibitors play key roles in almost all physiological processes. Proteinase inhibitors are present in all tissues and body fluids. They interfere with the activity of proteinases and thus maintain homeostasis. The main role of proteinase inhibitors in the reproductive tract is the inactivation of prematurely released hydrolytic enzymes from damaged spermatozoa and the protection of reproductive tracts and spermatozoa against proteolytic degradation. In the boar reproductive system, acrosin inhibitors are found in seminal plasma and on spermatozoa. The amino acid sequence of seminal plasma and sperm-associated acrosin inhibitors is 90% identical, and their biochemical properties have been completely resolved. However, their origin and localization have not been fully elucidated. Using rabbit polyclonal antibody, we have studied the expression and localization of the seminal plasma acrosin inhibitor in the boar reproductive tract. The antibody recognizes a 12-kDa band in extracts from the cauda epididymidis, seminal vesicles, prostate, and Cowper's glands, and immunofluorescence has revealed the acrosin inhibitor in the epithelium and lumen of these organs. Gene expression of the acrosin inhibitor has been studied by reverse transcription together with the polymerase chain reaction. Acrosin inhibitor mRNA transcript is detectable in the epididymis, seminal vesicles, prostate, and Cowper's glands. The antibody has localized the acrosin inhibitor on the surface of epididymal and ejaculated spermatozoa in the acrosomal region. In extracts from epididymal and ejaculated spermatozoa, the specific antibody recognizes acrosin inhibitor at 8 kDa and 12 kDa. The presence of acrosin inhibitor on the sperm surface as a protective molecule for receptors mediating the sperm-zona pellucida binding suggests that it is crucial for the reproductive process.
authors.affiliation: Laboratory of Diagnostics for Reproductive Medicine, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v.v.i., Vídenská 1083, 142 20, Prague, Czech Republic.
authors.forename: Nina
authors.initials: N
authors.lastname: Davidová
authors.forename: Vera
authors.initials: V
authors.lastname: Jonáková
authors.forename: Pavla
authors.initials: P
authors.lastname: Manásková-Postlerová
mesh_terms: D000177:Acrosome / Q000378:metabolism; D000818:Animals; D002030:Bulbourethral Glands / Q000378:metabolism*; D004822:Epididymis / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism; D012098:Reproduction; D012661:Semen / Q000378:metabolism; D029607:Seminal Vesicle Secretory Proteins / Q000096:biosynthesis*; D012669:Seminal Vesicles / Q000378:metabolism; D013552:Swine; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000096:biosynthesis*; D015044:Zona Pellucida / Q000378:metabolism
chemical_list: D011480:Protease Inhibitors; D029607:Seminal Vesicle Secretory Proteins; C087994:acrosin inhibitor, porcine; D014359:Trypsin Inhibitor, Kazal Pancreatic