title: Generation of functional ventricular heart muscle from mouse ventricular progenitor cells.
abstract: The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells. Genome-wide profiling of coding and noncoding transcripts revealed distinct molecular signatures of these progenitor populations. We further identify a committed ventricular progenitor cell in the Islet 1 lineage that is capable of limited in vitro expansion, differentiation, and assembly into functional ventricular muscle tissue, representing a combination of tissue engineering and stem cell biology.
authors.affiliation: Cardiovascular Research Center, Massachusetts General Hospital, Charles River Plaza, CPZN 3200, 185 Cambridge Street, Boston, MA 02114-2790, USA.
authors.forename: Ibrahim J
authors.initials: IJ
authors.lastname: Domian
authors.forename: Murali
authors.initials: M
authors.lastname: Chiravuri
authors.forename: Peter
authors.initials: P
authors.lastname: van der Meer
authors.forename: Adam W
authors.initials: AW
authors.lastname: Feinberg
authors.forename: Xi
authors.initials: X
authors.lastname: Shi
authors.forename: Ying
authors.initials: Y
authors.lastname: Shao
authors.forename: Sean M
authors.initials: SM
authors.lastname: Wu
authors.forename: Kevin Kit
authors.initials: KK
authors.lastname: Parker
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Chien
mesh_terms: D000200:Action Potentials; D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D015870:Gene Expression; D006321:Heart / Q000196:embryology; D006352:Heart Ventricles / Q000166:cytology* / Q000196:embryology; D051379:Mice; D008822:Mice, Transgenic; D024510:Muscle Development; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D023822:Tissue Engineering; D016276:Ventricular Function