[Colon adenoma detection using Kubelka-Munk spectral function of DNA and protein bands].
Differential diagnosis of human colon adenoma was studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 590 to 1 064 nm pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log [f(r infinity)] of the DNA absorption bands at 260 nm between normal and adenomatous colon epithelial tissues, and the differences were 218% (p < 0.05) and 68.5% (p < 0.05) respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log [f(r infinity)] of the protein absorption bands at 280 nm between normal and adenomatous colon epithelial tissues, and the differences were 208% (p < 0.05) and 59.0% (p < 0.05) respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log [f(r infinity)] of the beta-carotene absorption bands at 480 nm between normal and adenomatous colon epithelial tissues, and the differences were 41.7% (p < 0.05) and 32.9% (p < 0.05) respectively. Obviously, pathological changes of colon epithelial tissues were induced so that there were significant changes in the contents of the DNA, protein and beta-carotene of colon epithelial tissues. The conclusion can be applied to rapid, low-cost and noninvasive optical biopsy of colon adenoma, and provides a useful reference.
Wei
Hua-Jiang
HJ
Key Laboratory of Laser Life Science and Institute of Laser Life Science of Ministry of Education, South China Normal University, Guangzhou 510631, China. weihj@scnu.edu.cn
Guo
Zhou-Yi
ZY
Xie
Shu-Sen
SS
He
Bo-Hua
BH
Li
Li-Bo
LB
Chen
Xue-Mei
XM
Wu
Guo-Yong
GY
Lu
Jian-Jun
JJ
D000042:Absorption; D000236:Adenoma / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D003110:Colonic Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D003937:Diagnosis, Differential; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D006801:Humans; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D013057:Spectrum Analysis
D011506:Proteins; D004247:DNA