Molecular characterisation of PFGE non-typable methicillin-resistant Staphylococcus aureus in The Netherlands, 2007.
In 2007 in The Netherlands, 30% of all human isolates of methicillin-resistant Staphylococcus aureus (MRSA) sent to the National Institute for Public Health and the Environment could not be typed by pulsed-field gel electrophoresis (non-typable (NT)-MRSA). Molecular characterisation of the NT-MRSA isolates revealed 27 different spa types and two distinct SCCmec types, type IV and V. All NT-MRSA isolates were closely related based on spa and multi-locus sequence typing and belonged to the ST398 lineage. The rapid increase of NT-MRSA (ST398) isolates over the last years shows the importance of this relatively new clonal lineage.
Huijsdens
X W
XW
National Institute for Public Health and the Environment, Bilthoven, The Netherlands. Xander.Huijsdens@rivm.nl
Bosch
T
T
van Santen-Verheuvel
M G
MG
Spalburg
E
E
Pluister
G N
GN
van Luit
M
M
Heck
M E O C
ME
Haenen
A
A
de Neeling
A J
AJ
D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000302:isolation & purification*; D015336:Molecular Probe Techniques; D009426:Netherlands