Perturbation of survivin expression affects chromosome alignment and spindle checkpoint in mouse oocyte meiotic maturation.
Survivin is a member of inhibitors of apoptosis proteins (IAPs), which have multiple regulatory functions in mitosis, but its roles in meiosis remain unknown. Here, we report its expression, localization and functions in mouse oocyte meiosis. Survivin displayed a maximal expression level in GV stage, and then gradually decreased from Pro-MI to MII stages. Immunofluorescent staining showed that survivin was restricted to the germinal vesicle, associated with centromeres from pro-metaphase I to metaphase I stages, distributed at the midzone and midbody of anaphase and telophase spindles, and located to centromeres at metaphase II stages. Depletion of survivin by antibody injection and morpholino injection resulted in severe chromosome misalignment, precocious polar body extrusion, and larger-than-normal polar bodies. Overexpression of survivin resulted in severe chromosome misalignment and prometaphase I or metaphase I arrest in a large proportion of oocytes. Our data suggest that survivin is required for chromosome alignment and that it may regulate spindle checkpoint activity during mouse oocyte meiosis.
Sun
Shao-Chen
SC
Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning, China.
Wei
Liang
L
Li
Mo
M
Lin
Sheng-Li
SL
Xu
Bao-Zeng
BZ
Liang
Xing-Wei
XW
Kim
Nam-Hyung
NH
Schatten
Heide
H
Lu
Sheng-Sheng
SS
Sun
Qing-Yuan
QY
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D002875:Chromosomes / Q000502:physiology*; D051034:Inhibitor of Apoptosis Proteins; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism*; D009025:Morpholines / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism*; D012097:Repressor Proteins; D000077022:Survivin
D000906:Antibodies; C117749:Birc5 protein, mouse; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009025:Morpholines; D012097:Repressor Proteins; D000077022:Survivin