Binding and elution behavior of proteins on strong cation exchangers.
This work provides a broad survey of binding and elution behavior of proteins on strong cation exchangers. Four proteins comprising two monoclonal antibodies, lysozyme, and cytochrome c were used as models in the investigation. Seven chromatography resins with different base matrices were compared. Dynamic binding capacity as a function of salt concentration was examined for a monoclonal antibody and lysozyme. Elution behavior as a function of gradient slope was modeled to determine the characteristic charge, essentially a measure of the number of sites involved in binding, for each protein on each resin. Trends with respect to dynamic binding capacity and elution behavior are analyzed and discussed.
Pabst
Timothy M
TM
Pfizer Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA.
Suda
Eric J
EJ
Thomas
Kristin E
KE
Mensah
Paul
P
Ramasubramanyan
Natraj
N
Gustafson
Mark E
ME
Hunter
Alan K
AK
D000818:Animals; D002411:Cation Exchange Resins / Q000737:chemistry*; D002460:Cell Line; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D006736:Horses; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*
D002411:Cation Exchange Resins; D011506:Proteins