title: Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1.
abstract: We proposed a kinetic simulation model of xylose metabolism in Lactococcus lactis IO-1 that describes the dynamic behavior of metabolites using the simulator WinBEST-KIT. This model was developed by comparing the experimental time-course data of metabolites in batch cultures grown in media with initial xylose concentrations of 20.3-57.8 g/l with corresponding calculated data. By introducing the terms of substrate activation, substrate inhibition, and product inhibition, the revised model showed a squared correlation coefficient (r2) of 0.929 between the experimental time-course of metabolites and the calculated data. Thus, the revised model is assumed to be one of the best candidates for kinetic simulation describing the dynamic behavior of metabolites. Sensitivity analysis revealed that pyruvate flux distribution is important for higher lactate production. To confirm the validity of our kinetic model, the results of the sensitivity analysis were compared with enzyme activities observed during increasing lactate production by adding natural rubber serum powder to the xylose medium. The experimental results on pyruvate flux distribution were consistent with the prediction by sensitivity analysis.
authors.affiliation: Laboratory of Microbial Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Mugihito
authors.initials: M
authors.lastname: Oshiro
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shinto
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Tashiro
authors.forename: Noriko
authors.initials: N
authors.lastname: Miwa
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okamoto
authors.forename: Ayaaki
authors.initials: A
authors.lastname: Ishizaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003198:Computer Simulation; D007700:Kinetics; D013294:Lactococcus lactis / Q000145:classification / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D019289:Pyruvic Acid / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D012984:Software; D013045:Species Specificity; D014994:Xylose / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D019289:Pyruvic Acid; D014994:Xylose