title: Sensitive determination of verticine and verticinone in Bulbus Fritillariae by ionic liquid assisted capillary electrophoresis-electrochemiluminescence system.
abstract: CE/Ru(bpy)(3)(2+) electrochemiluminescence (ECL) system with the assistance of ionic liquids (ILs) was successfully established for sensitive determination of verticine and verticinone in Bulbus Fritillariae for the first time. Migration behavior of alkaloid largely relies on the hydrogen bonding interactions between alkyl imidazolium cations in ILs and the alkaloids. Running buffer containing 40 mmol/L 1-butyl-3-methylimidazolium tetrafluoroborate (BMImBF(4)) IL-8 mmol/L phosphate resulted in significant changes in separation selectivity for alkaloids with similar structures. The highest sensitivity of the detection was obtained by maintaining the detection potential at 1.2V. Under the optimized conditions, relative standard derivations of the ECL intensity and the migration time were 3.27 and 2.84% for verticine and 4.42 and 1.69% for verticinone, respectively. The standard curves were linear between 1x10(-8) and 1x10(-6) mol/L for verticine and between 5x10(-8) and 1x10(-6) mol/L for verticinone, respectively. Detection limits of 1.25x10(-10) mol/L for verticine and 1x10(-10) mol/L for verticinone were obtained (S/N=3). Developed method was successfully applied to determine the amounts of alkaloids in Bulbus Fritillariae.
authors.affiliation: Department of Applied Chemistry, Changchun Institute of Technology, Changchun, China. gaoy@ciac.jl.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Xu
authors.forename: Bingyan
authors.initials: B
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiang
mesh_terms: D002595:Cevanes / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D031410:Fritillaria / Q000737:chemistry* / Q000145:classification; D052578:Ionic Liquids / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D002595:Cevanes; D052578:Ionic Liquids; D010936:Plant Extracts; C014242:verticine