title: FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction.
abstract: A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nonaka
authors.forename: Sho-hei
authors.initials: SH
authors.lastname: Fujishima
authors.forename: Sho-hei
authors.initials: SH
authors.lastname: Uchinomiya
authors.forename: Akio
authors.initials: A
authors.lastname: Ojida
authors.forename: Itaru
authors.initials: I
authors.lastname: Hamachi
mesh_terms: D001665:Binding Sites; D008958:Models, Molecular; D015394:Molecular Structure; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D009842:Oligopeptides; D010455:Peptides; D011506:Proteins; C085706:FLAG peptide