title: Involvement of estrogen receptor beta in the induction of polyovular follicles in mouse ovaries exposed neonatally to diethylstilbestrol.
abstract: Natural and synthetic estrogens, Including diethylstilbestrol (DES), given during the critical period of newborn life Induce abnormalities in ovaries of mice. Induction of polyovular follicles (PFs) containing two or more oocytes in a follicle is one example. In this study, the involvement of estrogen receptor subtypes ERalpha and ERbeta in induction of PFs by neonatal treatment with DES was analyzed by using ERalpha knockout (alphaERKO) and ERbeta knockout (betaERKO) mice. Ovaries of mice injected with 3 microg DES for 5 days from the day of birth were examined histologically from 10 to 60 days of age, and the expression of genes involved in folliculogenesis was analyzed by real-time quantitative PCR. The PF Incidence (percent of PFs per 100 follicles greater than 50 microm in diameter) in the ovary of alphaERKO mice treated with DES was not different from that in the DES-treated wild-type mice. However, neonatal DES treatment did not increase the PF incidence in betaERKO mice, suggesting that PFs were induced by DES through ERbeta but not ERalpha. The expression of bone morphogenetic protein 15, growth differentiation factor 9, inhibin-alpha, Müllerian inhibiting substance, and other genes in the ovaries of DES-treated betaERKO mice was not different from that in the ovaries of DES-treated wild-type mice. These results indicate that ERbeta but not ERalpha is essential for DES to Induce PFs in mice.
authors.affiliation: Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kirigaya
authors.forename: Hannah
authors.initials: H
authors.lastname: Kim
authors.forename: Shinji
authors.initials: S
authors.lastname: Hayashi
authors.forename: Pierre
authors.initials: P
authors.lastname: Chambon
authors.forename: Hajime
authors.initials: H
authors.lastname: Watanabe
authors.forename: Taisen
authors.initials: T
authors.lastname: Lguchi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Sato
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D004054:Diethylstilbestrol / Q000009:adverse effects*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D006080:Ovarian Follicle / Q000187:drug effects*
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004054:Diethylstilbestrol