Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan.
Two clinical Aeromonas strains (A2-50(T) and A2-67(T)) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on their 16S rRNA gene sequences, which showed similarities of 99.6-99.8 % to those of the type strains of Aeromonas caviae, A. trota and A. aquariorum. The rpoD phylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species being A. caviae, the type strain of which showed 93.2 % similarity (56 bp differences) to strain A2-50(T) and 92.2 % (63 bp differences) to strain A2-67(T). A multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) confirmed that the two strains formed independent phylogenetic lineages within the genus. These data, together with phenotypic characterization and DNA-DNA reassociation results, revealed that these strains represent novel Aeromonas species, for which the names Aeromonas taiwanensis sp. nov. (type strain A2-50(T) =CECT 7403(T) =LMG 24683(T)) and Aeromonas sanarellii sp. nov. (type strain A2-67(T) =CECT 7402(T) =LMG 24682(T)) are proposed.
Alperi
Anabel
A
Unit of Microbiology, Rovira i Virgili University, IISPV, Sant Llorenç 21, 43201 Reus, Spain.
Martínez-Murcia
Antonio J
AJ
Molecular Diagnostics Center (MDC), Biomolecular Technologies SL, and Miguel Hernández University, 03300 Orihuela (Alicante), Spain.
Ko
Wen-Chien
WC
Department of Medicine, National Cheng Kung University, Tainan, Taiwan ROC.
Monera
Arturo
A
Molecular Diagnostics Center (MDC), Biomolecular Technologies SL, and Miguel Hernández University, 03300 Orihuela (Alicante), Spain.
Saavedra
Maria J
MJ
Department of Veterinary Sciences, CECAV - University of Trás-os-Montes e Alto Douro, Vila Real, Portugal.|Molecular Diagnostics Center (MDC), Biomolecular Technologies SL, and Miguel Hernández University, 03300 Orihuela (Alicante), Spain.
Figueras
Maria J
MJ
Unit of Microbiology, Rovira i Virgili University, IISPV, Sant Llorenç 21, 43201 Reus, Spain.
D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013624:Taiwan
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S