Adult rat mesenchymal stem cells differentiate into neuronal-like phenotype and express a variety of neuro-regulatory molecules in vitro.
Bone marrow stromal stem cells (MSCs), which normally differentiate into mesenchymal derivatives, have recently reported to trans-differentiate into neurons. However, the findings from different groups and interpretations have been challenged. The purpose of this paper is to re-evaluate the phenomenon of neuronal trans-differentiation of MSCs and compare the expression levels of neurotrophins in rMSCs and neuronal-like phenotypes derived from rMSCs. We put rMSCs in 2-mercaptoethanol and 2% dimethylsulfoxide for 5h. Then, the cells were transferred to neuronal induction media composed of DMEM+10%FBS, 10ug/L basic fibroblast growth factor, 10ug/L human epidermal growth factor, 1mmol dibutyryl cyclicn AMP and 0.5mmol isobutylmethylxanthine for 7 days and 14 days. The study demonstrated that the level of BDNF, NGF, NT3, CNTF and GDNF of rMSCs is remarkably higher in rMSCs than the neuronal-like phenotypes, especially CNTF. The expression level of these neurotrophins did not change significantly after enduring induction. We believed that rMSCs can trans-differentiate into neuronal-like phenotype under certain conditions. The non-induced rMSCs has a dynamic expression profile of neurotrophins and may serves as a better tool than the trans-differentiated rMSCs for transplant therapy.
Jiang
Junjian
J
Department of Hand Surgery, Huashan Hospital, Fudan University Shanghai 200040, China.
Lv
Zhongwei
Z
Gu
Yudong
Y
Li
Jifeng
J
Xu
Lei
L
Xu
Wengdong
W
Lu
Jiuzhuo
J
Xu
Jianguang
J
D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar
D015703:Antigens, CD; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins