Reverse genetics for functional genomics of phytopathogenic fungi and oomycetes.
Sequencing of over 40 fungal and oomycete genomes has been completed. The next major challenge in modern fungal/oomycete biology is now to translate this plethora of genome sequence information into biological functions. Reverse genetics has emerged as a seminal tool for functional genomics investigations. Techniques utilized for reverse genetics like targeted gene disruption/replacement, gene silencing, insertional mutagenesis, and targeting induced local lesions in genomes will contribute greatly to the understanding of gene function of fungal and oomycete pathogens. This paper provides an overview on high-throughput reverse genetics approaches to decode fungal/oomycete genomes.
Bhadauria
Vijai
V
The MOA Key Laboratory of Molecular Plant Pathology, Department of Plant Pathology, China Agricultural University, Beijing 100193, China.
Banniza
Sabine
S
Wei
Yangdou
Y
Peng
You-Liang
YL