A microscale approach for predicting the performance of chromatography columns used to recover therapeutic polyclonal antibodies.
A microscale approach is described which screens conditions for recovering polyclonal antibodies from ovine sera by mixed-mode cation-exchange chromatography. The impact of pH and loading buffer salt concentration were assessed using robotically operated 20microL packed pipette tips. Low salt concentrations delivered capacities up to 41mg/mL, while only half this level was obtained at high salt concentrations. Two of the screened conditions were then tested in a 10mL packed bed and overall trends in capacity, yield and purity were found to be retained. Microscale pipette tips thus provided a useful basis for the rapid, approximate definition of a chromatography design space.
Chhatre
Sunil
S
The Advanced Centre for Biochemical Engineering, University College London, Gower Street, London WC1E 7JE, UK. sunil.chhatre@ucl.ac.uk
Bracewell
Daniel G
DG
Titchener-Hooker
Nigel J
NJ
D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D044967:Serum / Q000737:chemistry; D012756:Sheep
D000906:Antibodies