3D microfabricated bioreactor with capillaries.
We present in this paper the implementation of an innovative three dimensional (3D) microfabrication technology coupled with numerical simulation to enhance the mass transport in 3D cell culture. The core of this microfabrication technology is a high-resolution projection micro stereolithography (PmicroSL) using a spatial light modulator as a dynamic mask which enables a parallel fabrication of highly complex 3D microstructures. In this work, a set of poly (ethylene glycol) microfabricated bioreactors are demonstrated with PmicroSL technology. We observed both experimentally and numerically the regulation of metabolism and the growth of yeast cells by controlling the density of micro-capillaries. Further development of these 3D microfabricated bioreactors is expected to provide artificially constructed tissues for clinical applications.
Xia
Chunguang
C
Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 158 Mechanical Engineering Building, MC-244, 1206 West Green Street, Urbana, IL 61801-2906, USA.
Fang
Nicholas X
NX
D019149:Bioreactors; D004058:Diffusion; D000431:Ethanol / Q000737:chemistry; D055616:Microtechnology / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry
D000431:Ethanol; D011092:Polyethylene Glycols