title: Phylogenetic analysis and characterization of Korean orf virus from dairy goats: case report.
abstract: An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain (ORF/09/Korea), the sequences of the major envelope protein (B2L) and orf virus interferon resistance (VIR) genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF/09/Korea strain was closest to the isolates (Taiping) from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea.
authors.affiliation: Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, 480 Anyang-6-Dong, Anyang, 430-824, Republic of Korea. jku0622@nvrqs.go.kr
authors.forename: Jae-Ku
authors.initials: JK
authors.lastname: Oem
authors.forename: In-Soon
authors.initials: IS
authors.lastname: Roh
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Lee
authors.forename: Kyoung-Ki
authors.initials: KK
authors.lastname: Lee
authors.forename: Hye-Ryoung
authors.initials: HR
authors.lastname: Kim
authors.forename: Young-Hwa
authors.initials: YH
authors.lastname: Jean
authors.forename: O-Soo
authors.initials: OS
authors.lastname: Lee
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks; D004474:Ecthyma, Contagious / Q000453:epidemiology* / Q000821:virology; D015511:Goat Diseases / Q000453:epidemiology* / Q000821:virology; D006041:Goats; D008046:Lip / Q000821:virology; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012867:Skin / Q000821:virology; D013624:Taiwan; D014759:Viral Envelope Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins