title: Stable carbon isotope fractionation of 1,2-dichloropropane during dichloroelimination by Dehalococcoides populations.
abstract: The isotope fractionation of 1,2-dichloropropane (1,2-D) during dichloroelimination to propene by Dehalococcoides populations was explored in laboratory experiments in order to provide data for the characterization of the fate of 1,2-D in heterogeneous subsurface systems. Compound specific stable carbon isotope analysis (CSIA) was used to determine the bulk enrichment factors (epsilonbulk), reactive position specific enrichment factors (epsilonreactive), and apparent kinetic isotope effect (AKIE) values for 1,2-D dichloroelimination in two distinct Dehalococcoides-containing cultures. The epsilonbulk factors calculated in the two cultures were statistically identical, -10.8 +/- 0.9 and -11.3 +/- 0.8 per thousand, even though the cultures were derived from geographically distinct locations. AKIE values for 1,2-D dichloroelimination assuming stepwise and concerted reaction mechanisms were approximately 1.033 and 1.017, respectively. These values are within the range of previously reported values for dichloroelimination reactions and were equivalent to values reported for biotic 1,2-dichloroethane and abiotic 1,1,2,2,-tetrachloroethane and pentachloroethane dichloroelimination reactions.
authors.affiliation: School of Civil and Environmental Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, Georgia 30332, USA.
authors.forename: Kelly E
authors.initials: KE
authors.lastname: Fletcher
authors.forename: Frank E
authors.initials: FE
authors.lastname: Löffler
authors.forename: Hans-Hermann
authors.initials: HH
authors.lastname: Richnow
authors.forename: Ivonne
authors.initials: I
authors.lastname: Nijenhuis
mesh_terms: D000475:Alkenes / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002247:Carbon Isotopes; D002384:Catalysis; D005591:Chemical Fractionation / Q000379:methods*; D007700:Kinetics; D011407:Propane / Q000031:analogs & derivatives* / Q000302:isolation & purification; D012044:Regression Analysis
chemical_list: D000475:Alkenes; D002247:Carbon Isotopes; C013658:propylene; C004765:propylene dichloride; D011407:Propane