Antimicrobial resistance and virulence genes in Salmonella enterica isolates from dairy cows.
One hundred sixty Salmonella enterica isolates from clinically healthy dairy cows were assayed for antibiotic susceptibilities, the presence of class 1 integrons, antimicrobial resistance genes and virulence genes, and conjugal transfer of antimicrobial resistance determinants. One hundred nine (68%) of the Salmonella isolates were resistant to at least 1 antibiotic, and 14 isolates (9%) were multiresistant. The most prevalent resistance observed was to streptomycin (64%). Class 1 integrons were detected in only two Salmonella isolates (serovar Singapore and Derby), and both integrons harbored the same cassette content aadA2. The Derby class 1 integrons were associated with Salmonella genomic island 1-A. Most commonly found resistance genes were strA and strB (9.2%). None of class 1 integrons were horizontally transferred, and the resistance genes were successfully transferred from six (5.5%) Salmonella strains. One hundred fifty-nine isolates (98.8%) were positive to the invasion gene invA, whereas the virulence plasmid-associated genes spvC and pefA were found in only two (1.3%) and one (0.6%) Salmonella isolates, respectively.
Chuanchuen
Rungtip
R
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. rchuanchuen@yahoo.com
Ajariyakhajorn
Kittisak
K
Koowatananukul
Chailai
C
Wannaprasat
Wechsiri
W
Khemtong
Sirintip
S
Samngamnim
Sukuma
S
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D003227:Conjugation, Genetic; D003612:Dairying; D004197:Disease Reservoirs / Q000662:veterinary; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005517:Foodborne Diseases / Q000517:prevention & control; D018014:Gene Transfer Techniques; D019450:Genes, MDR; D014644:Genetic Variation; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction; D012007:Rectum / Q000382:microbiology; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D013785:Thailand; D014774:Virulence / Q000235:genetics*