title: Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway.
abstract: In the silkworm Bombyx mori, densovirus type 1 (BmDNV-1) replicates only in the midgut and causes fatal disease. Resistance to BmDNV-1 is determined by two genes, nsd-1 and Nid-1, respectively. Neither of them has been identified yet. We investigated the viral transcript by RT-PCR in inoculated silkworms carrying different sets of nsd-1 and Nid-1 genotype. BmDNV-1 transcript was not detected in nsd-1-carrying strains irrespective of existence of Nid-1 but clearly detected in strains carrying Nid-1 without nsd-1. The result suggests that nsd-1 blocks early step of infection. On the other hand, Nid-1 does not block cell and nucleus entry and viral transcription in nuclei but blocks later step in the viral infection cycle.
authors.affiliation: Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Kurako
authors.initials: K
authors.lastname: Kidokoro
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ito
authors.forename: Dorington O
authors.initials: DO
authors.lastname: Ogoyi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Abe
authors.forename: Kazuei
authors.initials: K
authors.lastname: Mita
authors.forename: Keiko
authors.initials: K
authors.lastname: Kadono-Okuda
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D017990:Densovirus / Q000472:pathogenicity*; D004064:Digestive System / Q000821:virology; D017344:Genes, Insect; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D007304:Insect Viruses / Q000472:pathogenicity