title: Evaluation of antidiabetic activity of polysaccharide isolated from Phellinus linteus in non-obese diabetic mouse.
abstract: Polysaccharide (PLP) isolated from Phellinus linteus inhibits tumor growth and metastasis by enhancing immune functions of macrophages, dendritic cells, NK cells, T cells, and B cells. Here, we report that PLP can inhibit the development of autoimmune diabetes in non-obese diabetic (NOD) mice. Although 80% of the NOD mice had developed diabetes by 24 weeks of age, none of the PLP-treated NOD mice developed diabetes. The mean blood glucose levels were 110mg/dl in PLP-treated mice and 499mg/dl in control NOD mice. Histological examination of the pancreatic islets revealed that most of the islets isolated from PLP-treated mice were less infiltrated with lymphocytes compared with those of control mice. Spleen cells from diabetic NOD mice could adaptively transfer diabetes into NOD/SCID mice, but those from PLP-treated NOD mice showed delayed transfer of diabetes. PLP inhibited the expression of inflammatory cytokines, including IFN-gamma, IL-2, and TNF-alpha by Th1 cells and macrophages, but up-regulated IL-4 expression by Th2 cells in NOD mice. PLP did not prevent streptozotocin-induced diabetic development in ICR mice. Taken together, these results suggest that PLP inhibits the development of autoimmune diabetes by regulating cytokine expression.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Republic of Korea.
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Jee Youn
authors.initials: JY
authors.lastname: Kim
authors.forename: Song-Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Hyung Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Young June
authors.initials: YJ
authors.lastname: Lee
authors.forename: Jieun
authors.initials: J
authors.lastname: Yun
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000276:immunology; D001835:Body Weight / Q000187:drug effects; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D018450:Disease Progression; D005260:Female; D005658:Fungi; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D016688:Mice, Inbred NOD; D010179:Pancreas / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000084682:Phellinus; D010936:Plant Extracts; D011134:Polysaccharides / Q000008:administration & dosage* / Q000009:adverse effects; D013311:Streptozocin / Q000008:administration & dosage; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology / Q000637:transplantation; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000637:transplantation
chemical_list: D001786:Blood Glucose; D016207:Cytokines; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D013311:Streptozocin