title: In vivo corrosion and corrosion protection of magnesium alloy LAE442.
abstract: The aim of this study was to investigate whether the extruded magnesium alloy LAE442 reacts in vivo with an appropriate host response and to investigate how an additional magnesium fluoride (MgF(2)) coating influences the in vivo corrosion rate. Forty cylinders were machined from extruded LAE442 and 20 of these were coated additionally with MgF(2) and implanted into the medial femur condyle of adult rabbits. Synchrotron-radiation-based X-ray computed micro-tomography (SRmicroCT) was used to quantitatively analyse corrosion non-destructively in vivo and comparisons were made to magnesium degradation rates based on area measurements of the remaining metal on uncalcified sections. Blood concentrations of the alloying elements were measured below toxicological limits. The MgF(2) layer was no longer detected after 4 weeks of implantation by particle-induced gamma emission, and the MgF(2) coating reduced the blood content of alloying elements during the first 6 weeks of implantation with no elevated fluoride concentration in the adjacent bone. Histopathological examinations of liver showed in 9 out of 40 cases minimal infiltrations of heterophil granulocytes of unknown origin (5 LAE442, 4 LAE442+MgF(2)). The kidneys were mainly regular in structure. The synovial tissue showed a granular cell infiltration as a temporary observation in the LAE442+MgF(2) group after 2 weeks. No subcutaneous gas cavities were observed clinically and on postoperative X-rays in all animals. All specimens were scanned by SRmicroCT at 2, 4, 6 and 12 weeks postoperatively before uncalcified sections were performed. All magnesium implants have been observed in direct bone contact and without a fibrous capsule. Localized pitting corrosion occurred in coated and uncoated magnesium implants. This study shows that the extruded magnesium alloy LAE442 provides low corrosion rates and reacts in vivo with an acceptable host response. The in vivo corrosion rate can be further reduced by additional MgF(2) coating.
authors.affiliation: Laboratory for Biomechanics and Biomaterials, Hannover Medical School, Anna-von-Borries-Strasse 1-7, 30625 Hannover, Germany. f.witte@web.de
authors.forename: F
authors.initials: F
authors.lastname: Witte
authors.forename: J
authors.initials: J
authors.lastname: Fischer
authors.forename: J
authors.initials: J
authors.lastname: Nellesen
authors.forename: C
authors.initials: C
authors.lastname: Vogt
authors.forename: J
authors.initials: J
authors.lastname: Vogt
authors.forename: T
authors.initials: T
authors.lastname: Donath
authors.forename: F
authors.initials: F
authors.lastname: Beckmann
mesh_terms: D000497:Alloys / Q000494:pharmacology*; D000818:Animals; D020099:Coated Materials, Biocompatible; D003343:Corrosion; D004602:Elements; D005260:Female; D005269:Femur / Q000187:drug effects / Q000473:pathology; D005459:Fluorides / Q000494:pharmacology; D006859:Hydrogen / Q000737:chemistry; D020007:Implants, Experimental; D008274:Magnesium / Q000494:pharmacology*; D017616:Magnesium Compounds / Q000494:pharmacology; D011817:Rabbits; D017356:Synchrotrons; D013997:Time Factors; D055114:X-Ray Microtomography
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D004602:Elements; D017616:Magnesium Compounds; C031288:magnesium fluoride; D006859:Hydrogen; D008274:Magnesium; D005459:Fluorides