title: Application of on-line electrochemistry/electrospray/tandem mass spectrometry to a quantification method for the antipsychotic drug zotepine in human serum.
abstract: A simple, rapid, and sensitive on-line liquid chromatographic electrochemistry/electrospray/tandem mass spectrometry (LC-EC/ESI-MS/MS) method for the determination of zotepine in human serum was developed using a new generated-electrochemically fragment ion, and was validated. A recent novel technique of LC-EC/ESI-MS/MS that combines LC-MS/MS and the on-line EC reaction is potentially applicable to developing a quantification method for drugs in biological samples. Newly formed products generated by the on-line EC cell are expected to provide appropriate precursor and product ions for the MS/MS determination method. This technique was successfully applied to a drug assay in a biological matrix. After adding imipramine (IS) to a 30-microL aliquot of human serum, the resulting sample was simply deproteinated with acetonitrile for a measurement. The analytical run time was 5 min. The calibration curve was linear in the concentration range of 10-2000 ng/mL. The intra-assay precision and accuracy were in the range of 1.8-8.9 and 98.4-113%, respectively.
authors.affiliation: Department of Applied Chemistry, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Nozaki
authors.forename: Issey
authors.initials: I
authors.lastname: Osaka
authors.forename: Hideya
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D014150:Antipsychotic Agents / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002417:Cattle; D003988:Dibenzothiepins / Q000097:blood*; D004563:Electrochemistry; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D009862:Online Systems; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D014150:Antipsychotic Agents; D003988:Dibenzothiepins; C022172:zotepine