Electrochemical immunoassay using magnetic beads for the determination of zearalenone in baby food: an anticipated analytical tool for food safety.
In this work, electrochemical immunoassay involving magnetic beads to determine zearalenone in selected food samples has been developed. The immunoassay scheme has been based on a direct competitive immunoassay method in which antibody-coated magnetic beads were employed as the immobilisation support and horseradish peroxidase (HRP) was used as enzymatic label. Amperometric detection has been achieved through the addition of hydrogen peroxide substrate and hydroquinone as mediator. Analytical performance of the electrochemical immunoassay has been evaluated by analysis of maize certified reference material (CRM) and selected baby food samples. A detection limit (LOD) of 0.011 microg L(-1) and EC(50) 0.079 microg L(-1) were obtained allowing the assessment of the detection of zearalenone mycotoxin. In addition, an excellent accuracy with a high recovery yield ranging between 95 and 108% has been obtained. The analytical features have shown the proposed electrochemical immunoassay to be a very powerful and timely screening tool for the food safety scene.
Hervás
Miriam
M
Departamento Quimica Analitica, Universidad de Alcala, Ctra. Madrid-Barcelona, Km. 33,600, E-28871 Alcala de Henares, Madrid, Spain.
López
Miguel Angel
MA
Escarpa
Alberto
A
D004563:Electrochemistry / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D007225:Infant Food / Q000032:analysis*; D008280:Magnetics; D015025:Zearalenone / Q000032:analysis*
D015025:Zearalenone